[Studies on the chemical constituents in root of Gentiana macrophylla from Shaanxi].
OBJECTIVE
To in vestigate the constituents in root of Gentiana macrophylla.


METHODS
Various column chromatographic techniques were used for isolation and purification of the principles. The structures were elucidated on the basis of spectral data (UV, IR, MS, 1H-, 13C-NMR etc.) and identified by comparing with standard substance.


RESULTS
Eight compounds were identified. Four compounds isolated from the chloroform fraction are: 5-carboxyl-3,4-dihydrogen-1H-2-benzopyran-1-one (1), erythrocentauric acid (2), roburic acid (3), oleanolic acid (4). Water fraction gave four known secoiridoid glucosides. They were: gentiopicroside (5), swertiamarine (6), sweroside (7), 6'-O-beta-D-glucosylgentiopicroside (8).


CONCLUSIONS
1 is a novel compound. It was named as erythrocentauric acid. 2 was isolated from genus Gentiana and 8 was isolated from G. macrophylla for the first time.
Chen
Qian-Liang
QL
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. xdyh@yahoo.com.cn
Shi
Zhang-Yan
ZY
Tu
Guang-Zhong
GZ
Sun
Wen-Ji
WJ
D030012:Gentiana / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000737:chemistry / Q000302:isolation & purification*; D049934:Isocoumarins / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
C516875:6'-O-beta-D-glucosylgentiopicroside; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D049934:Isocoumarins; C516874:erythrocentauric acid; C007623:erythrocentaurin