Qualitative simulation of the carbon starvation response in Escherichia coli.
In case of nutritional stress, like carbon starvation, Escherichia coli cells abandon their exponential-growth state to enter a more resistant, non-growth state called stationary phase. This growth-phase transition is controlled by a genetic regulatory network integrating various environmental signals. Although E. coli is a paradigm of the bacterial world, it is little understood how its response to carbon starvation conditions emerges from the interactions between the different components of the regulatory network. Using a qualitative method that is able to overcome the current lack of quantitative data on kinetic parameters and molecular concentrations, we model the carbon starvation response network and simulate the response of E. coli cells to carbon deprivation. This allows us to identify essential features of the transition between exponential and stationary phase and to make new predictions on the qualitative system behavior following a carbon upshift.
Ropers
Delphine
D
Institut National de Recherche en Informatique et en Automatique (INRIA), Unité de recherche Rhône-Alpes, 655 Avenue de l 'Europe, Montbonnot, 38334 Saint Ismier Cedex, France. Delphine.Ropers@inrialpes.fr
de Jong
Hidde
H
Page
Michel
M
Schneider
Dominique
D
Geiselmann
Johannes
J
D002244:Carbon / Q000378:metabolism*; D004278:DNA, Superhelical; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D016014:Linear Models
D004278:DNA, Superhelical; D002244:Carbon