title: The acute phase response alters cationic amino acid transporter expression in growing chickens (Gallus gallus domesticus).
abstract: The effect of an acute phase response (APR) on cationic amino acid transporter (CAT1-3) mRNA expression in liver, muscle, bursa and thymus was determined in broiler strain chickens. The APR was initiated by injecting Salmonella typhimurium lipopolysaccharide subcutaneously (LPS; 1 mg/kg bw). In Experiment 1, CAT1-3 mRNA expression was determined at multiple time points following LPS administration. LPS increased bursa and liver total and high affinity CAT mRNA expression (P<0.05) and transiently increased pectoralis total CAT mRNA expression (P<0.05). Total CAT mRNA expression in the thymus decreased 7.7-fold from 0 to 8 h after LPS injection (P<0.05). In Experiment 2, fasted chicks were uninjected or LPS-injected. LPS increased total and high affinity CAT mRNA 2-fold in both the bursa and liver (P<0.05) and did not change thymus total and high affinity CAT mRNA expression (P>0.05). LPS increased liver weight only (P<0.05) and did not alter the plasma lysine and arginine concentration (P>0.05). In Experiments 3 and 4, thymocyte proliferation and total protein content were dependent upon the media lysine concentration (P<0.001). The inability of the thymus to compete for lysine and arginine during the APR may limit the ability of thymocytes to develop during infections.
authors.affiliation: Department of Animal Science, University of California, Davis, CA 95616, USA.
authors.forename: Brooke D
authors.initials: BD
authors.lastname: Humphrey
authors.forename: Kirk C
authors.initials: KC
authors.lastname: Klasing
mesh_terms: D000210:Acute-Phase Reaction / Q000235:genetics* / Q000378:metabolism; D026923:Amino Acid Transport Systems, Basic / Q000187:drug effects / Q000235:genetics*; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D027201:Cationic Amino Acid Transporter 1 / Q000187:drug effects / Q000235:genetics; D027202:Cationic Amino Acid Transporter 2 / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens / Q000235:genetics* / Q000254:growth & development*; D004032:Diet; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007279:Injections, Subcutaneous; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008239:Lysine / Q000494:pharmacology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects
chemical_list: D026923:Amino Acid Transport Systems, Basic; D027201:Cationic Amino Acid Transporter 1; D027202:Cationic Amino Acid Transporter 2; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D012333:RNA, Messenger; C028784:phytohemagglutinin-P; D001120:Arginine; D008239:Lysine