The removal of Cremophor EL from paclitaxel for quantitative analysis by HPLC-UV.
A novel method for analysis of hydrophobic drug molecules in matrices that contain Cremophor EL (CrEL) is presented. The method utilized a precipitation technique involving mercuric chloride in a reaction with CrEL to form an insoluble complex in an ethanol matrix. The hydrophobic drug molecule was then analyzed by HPLC-UV without interference from CrEL. Nuclear magnetic resonance and infrared spectroscopy indicated that the mechanism of precipitation involves the reaction of mercuric chloride with the ether bond of CrEL. Analysis by HPLC with UV detection of paclitaxel and related substances was used to verify that the reaction is specific toward CrEL.
Perdue
James D
JD
Wilmington Analytical Development Laboratories, AAIPharma, 2320 Scientific Park Dr, Wilmington, NC 28405, USA.
Seaton
Pamela J
PJ
Tyrell
John A
JA
DeVido
Daniel R
DR
D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005990:Glycerol / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008627:Mercuric Chloride / Q000737:chemistry*; D017239:Paclitaxel / Q000032:analysis* / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000295:instrumentation / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000032:analysis* / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D014466:Ultraviolet Rays
D013501:Surface-Active Agents; D008627:Mercuric Chloride; C000515:cremophor EL; D017239:Paclitaxel; D005990:Glycerol