title: Kinetics of antiviral CD8 T cell responses during primary and post-vaccination secondary bovine respiratory syncytial virus infection.
abstract: We have measured antiviral CD8 T cells responses in bovine respiratory syncytial virus (bRSV) infected calves that had been immunized with either formalin-inactivated (FI) or live-attenuated (L) bRSV, with evidence of immunopathology following challenge of calves vaccinated with FI-bRSV. In all cases, bRSV infection induced potent pulmonary CD8 T cell responses. The kinetics of the post-challenge response in L-bRSV immunized animals was accelerated compared to the FI-bRSV and PBS groups, suggesting that only the L-bRSV vaccine, and not the FI-bRSV vaccine, had primed memory T cells. The differences between primary and post-vaccination secondary infection were very minor, in terms of the proliferation status of pulmonary CD8 T cells. Functional IFN-gamma+ CD8 responses were slightly higher in the FI-bRSV vaccinated animals. Furthermore, the existence of strong IFN-gamma+ CD8 responses in FI-bRSV vaccinated animals after challenge suggests (i) that these IFN-gamma+ responses in FI-bRSV immunized animals do not protect against immunopathology, and (ii) that Th-2 biased responses during bRSV challenge after vaccination with FI-bRSV have a limited impact on the CD8 responses in the bronchoalveolar lavage fluid. Thus, several response patterns (Th-l/Th-2) seem to co-exist during bRSV infection.
authors.affiliation: Animal Sciences Group, Wageningen UR, Lelystad, The Netherlands.
authors.forename: Adriaan F G
authors.initials: AF
authors.lastname: Antonis
authors.forename: Erwin A W
authors.initials: EA
authors.lastname: Claassen
authors.forename: Evert J
authors.initials: EJ
authors.lastname: Hensen
authors.forename: Raoul J
authors.initials: RJ
authors.lastname: de Groot
authors.forename: Jolanda D F
authors.initials: JD
authors.lastname: de Groot-Mijnes
authors.forename: Remco S
authors.initials: RS
authors.lastname: Schrijver
authors.forename: Robbert G
authors.initials: RG
authors.lastname: van der Most
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002417:Cattle; D002465:Cell Movement; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000096:biosynthesis; D008168:Lung / Q000276:immunology; D008213:Lymphocyte Activation; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*; D022261:Respiratory Syncytial Virus Vaccines / Q000276:immunology*; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology*; D014611:Vaccination
chemical_list: D022261:Respiratory Syncytial Virus Vaccines; D007371:Interferon-gamma