Regulation of magnesium-inhibited cation current by actin cytoskeleton rearrangement.
Magnesium-inhibited, non-selective cation current (I(MIC)) is activated by depletion of intracellular Mg(2+) and ATP. I(MIC) transports various divalent cations including Mg(2+) and Ca(2+), and is involved in cell viability. We investigated the effect of actin dynamics on I(MIC). Formation of a stable cortical actin network by calyculin A inhibited the activation of I(MIC), while the actin depolymerizing reagent, cytochalasin D, reversed the inhibition. Induction of a dense cortical actin layer by transfecting the constitutively active form of RhoA also inhibited the activation of I(MIC). These results suggest that the activation of I(MIC) may be dynamically regulated by actin cytoskeleton rearrangement.
Jeong
Soon Youn
SY
Department of Physiology, Sungkyunkwan University School of Medicine, Suwon 440-746, South Korea.
Shin
Sun Young
SY
Kim
Han-Seop
HS
Bae
Chang-Dae
CD
Uhm
Dae-Yong
DY
Park
Myung-Kyu
MK
Chung
Sungkwon
S
D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure*; D002412:Cations; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D019169:Jurkat Cells; D008274:Magnesium / Q000494:pharmacology*
D002412:Cations; D007473:Ion Channels; D008274:Magnesium