title: Genotoxic evaluation of the biocomponents of the cricket, Gryllus bimaculatus, using three mutagenicity tests.
abstract: The mutagenic potential of the extracted components of Gryllus bimaculatus, a species of cricket, was evaluated using short-term genotoxicity tests including the Ames, chromosome aberration, and micronuclei tests. In a Salmonella typhimurium assay, G. bimaculatus extract did not produce any mutagenic response in the absence or presence of S9 mix with TA98, TA100, TA1535, and TA1537. Chromosome aberration testing showed that G. bimaculatus had no significant effect on Chinese hamster ovary (CHO) cells. In the mouse micronucleus test, no significant alteration in occurrence of micronucleated polychromatic erythrocytes was observed in ICR male mice intraperitoneally administered with G. bimaculatus extract at doses of 15, 150, or 1500 mg/kg. These results indicate that G. bimaculatus extract exerts no mutagenic effect in these in vitro and in vivo systems.
authors.affiliation: Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA, Suwon, South Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Ahn
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Bae
authors.forename: In Sun
authors.initials: IS
authors.lastname: Kim
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Yoo
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Kwack
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Kim
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Kang Sun
authors.initials: KS
authors.lastname: Ryu
authors.forename: Hee Sam
authors.initials: HS
authors.lastname: Lee
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Iksoo
authors.initials: I
authors.lastname: Kim
authors.forename: Byung Mu
authors.initials: BM
authors.lastname: Lee
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D016466:CHO Cells; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D005202:Asia, Eastern; D006135:Gryllidae / Q000737:chemistry* / Q000378:metabolism; D019476:Insect Proteins / Q000633:toxicity*; D008297:Male; D008518:Medicine, East Asian Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D012485:Salmonella typhi / Q000187:drug effects / Q000235:genetics
chemical_list: D019476:Insect Proteins