title: Heterogeneous t(4;11) fusion transcripts in two infants with acute lymphoblastic leukemia.
abstract: An RT-PCR assay detected the t(4;11) translocation in two infants with acute lymphoblastic leukemia (ALL). Case P76 was a 10-month-old, female infant, who presented with a WBC of 137.4 x 10(9)/l and a pre-pre-B ALL immunophenotype. Case P120 was a 6-month-old female infant, with a WBC > 615 x 10(9)/l and a pre-pre-B ALL immunophenotype. RT-PCR of cDNA from both these cases generated a 656 bp and a 542 bp respectively, which sequencing confirmed as t(4;11) fusion transcripts. The primers and conditions selected for this assay are compatible with a one-step multiplex PCR for the main translocations in childhood ALL.
authors.affiliation: Allergy & Immunology Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia. gillhk@imr.gov.my
authors.forename: Harvindar Kaur
authors.initials: HK
authors.lastname: Gill
authors.forename: Sew Keoh
authors.initials: SK
authors.lastname: Ten
authors.forename: Jasbir Singh
authors.initials: JS
authors.lastname: Dhaliwal
authors.forename: Sarah
authors.initials: S
authors.lastname: Moore
authors.forename: Roshida
authors.initials: R
authors.lastname: Hassan
authors.forename: Faraiza Abdul
authors.initials: FA
authors.lastname: Karim
authors.forename: Zubaidah
authors.initials: Z
authors.lastname: Zakaria
authors.forename: Shahnaz
authors.initials: S
authors.lastname: Murad
authors.forename: Mahfuzah
authors.initials: M
authors.lastname: Mohamed
authors.forename: Hishamshah Mohamad
authors.initials: HM
authors.lastname: Ibrahim
authors.forename: Eni Juraida Abdul
authors.initials: EJ
authors.lastname: Rahman
mesh_terms: D001483:Base Sequence; D001854:Bone Marrow Cells / Q000473:pathology; D002880:Chromosomes, Human, Pair 11; D002894:Chromosomes, Human, Pair 4; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic
chemical_list: D004273:DNA, Neoplasm