Oxidative stress provokes atherogenic changes in adipokine gene expression in 3T3-L1 adipocytes.
Increased oxidative stress has been associated with obesity-related disorders. In this study, we investigated how oxidative stress, in different ways of exposure, regulates gene expression of various adipokines in 3T3-L1 adipocytes. Exposure to 100-500microM H(2)O(2) for 10min, as well as exposure to 5-25mU/ml glucose oxidase for 18h, similarly decreased adiponectin, leptin, and resistin mRNAs, and increased plasminogen activator inhibitor-1 mRNA. Secretion levels of adipokines were also changed by oxidative stress in parallel with mRNA expression levels. Although a peak increase in plasminogen activator inhibitor-1 mRNA was achieved between 4 and 8h after exposure to H(2)O(2) for 10min, significant decreases in adiponectin and resistin mRNA were observed after 16h, while leptin mRNA was decreased earlier. Our results suggest that oxidative stress, even of short duration, has a significant impact on the regulation of various adipokine gene expressions favoring atherosclerosis.
Kamigaki
Mitsunori
M
First Department of Medicine, Hokkaido University School of Medicine, Sapporo 060-8638, Japan.
Sakaue
Shinji
S
Tsujino
Ichizo
I
Ohira
Hiroshi
H
Ikeda
Daisuke
D
Itoh
Naofumi
N
Ishimaru
Shinji
S
Ohtsuka
Yoshinori
Y
Nishimura
Masaharu
M
D041721:3T3-L1 Cells; D000111:Acetylcysteine / Q000494:pharmacology; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D000818:Animals; D050197:Atherosclerosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D005949:Glucose Oxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020738:Leptin / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress; D036361:Peptide Hormones / Q000235:genetics* / Q000378:metabolism; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D052243:Resistin / Q000235:genetics / Q000378:metabolism
D052242:Adiponectin; D020738:Leptin; D036361:Peptide Hormones; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D052243:Resistin; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D000111:Acetylcysteine