Development of a method to analyze single cell activity by using dielectrophoretic levitation.
In cell fusion and genetic recombination, although the activity of single cells is extremely important, there is no method to analyze single cell activity. Development of a quick analyzing method for single cell activity is desired in various fields. Dielectrophoresis (DEP) refers to the force exerted on the induced dipole moment of an uncharged dielectric and/or conductive particle by a nonuniform electric field. By applying DEP, we obtained experimentally a relationship between the cell activity and the dielectric property, Re[K(omega)], and examined how to evaluate the single cell activity by measuring Re[K(omega)] of a single cell. A cone and plate electrode geometry was adapted in order to achieve the feedback-controlled DEP levitation. The single cell is exposed to a nonuniform field induced by the cone and plate electrode, and a more polarizable cell is moved to the direction of the cone electrode by the DEP force. The cell settles in the position where the DEP force and gravity are balanced by controlling applied voltage. This settled position, measured on the center axis of the cone electrode, depended on the dielectric constant of the cell. From these results, the relationship between the specific growth rates in cell growth phase and the dielectric properties Re[K(omega)] was obtained. Furthermore, the effect on the cell activity of various stresses, such as concentration of carbon dioxide, temperature, etc., was examined.
Hakoda
M
M
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu City, Japan. hakoda@bce.gunma-u.ac.jp
Hachisu
T
T
Wakizaka
Y
Y
Mii
S
S
Kitajima
N
N
D000818:Animals; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D002459:Cell Fusion; D002460:Cell Line; D002468:Cell Physiological Phenomena; D049109:Cell Proliferation; D004558:Electric Stimulation; D004566:Electrodes; D004594:Electrophysiology; D005246:Feedback; D051379:Mice; D008954:Models, Biological; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology