Expression of small stress protein hsp20 gene in the maturing rat testis.
In this study, we cloned a cDNA that encodes a small heat shock protein, Hsp20 (alphaB crystallin-related protein), from a maturing rat testis by means of differential display. The full-length cDNA sequence was completely identical to that registered in the DNA databank. The expression of Hsp20 gene was detected strongly in the heart and slightly in the testis of a 9-week-old rat. The expression of Hsp20 increased gradually from three weeks to 9 weeks, and the strongest expression was observed in the testis at week fifteen. The expression was localized in spermatocytes and round spermatids. The gene expression was not affected by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) when it was administered into male rats during the nursling period.
Yamano
Yoshiaki
Y
Laboratory of Veterinary Biochemistry, Faculty of Agriculture, Tottori University, Japan.
Ohyama
Kenji
K
Ohta
Masanori
M
Nakamura
Juri
J
Morishima
Isao
I
D000367:Age Factors; D000818:Animals; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D050886:HSP20 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000008:administration & dosage / Q000633:toxicity; D051381:Rats / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
D018076:DNA, Complementary; D050886:HSP20 Heat-Shock Proteins; D000072317:Polychlorinated Dibenzodioxins