Virucidal activity of a new hand disinfectant with reduced ethanol content: comparison with other alcohol-based formulations.
A new formula with reduced ethanol content (55%) in combination with 10% propan-1-ol, 5.9% propan-1.2-diol, 5.7% butan-1.3-diol and 0.7% phosphoric acid exhibited a broad spectrum of virucidal activity. In quantitative suspension tests, with and without protein load, this formulation reduced the infectivity titre of seven enveloped (influenza A and B, herpes simplex 1 and 2, bovine corona, respiratory syncytial, vaccinia, hepatitis B, bovine viral diarrhoea) and four non-enveloped (hepatitis A, polio, rota, feline calici) viruses >10(3)-fold within 30s. In comparative testing, only 95% ethanol showed similar levels of activity. In fingerpad tests, the formulation produced a log10 reduction factor of the titre of poliovirus type 1 (Sabin) of 3.04 in 30s compared with 1.32 by 60% propan-2-ol. Testing against feline calicivirus produced a log10 reduction factor of 2.38 by the test formulation; in contrast, the log10 reduction factors with 70% ethanol and 70% propan-1-ol were 0.68 and 0.70, respectively.
Kramer
A
A
Institute of Hygiene and Environmental Medicine, Ernst Moritz Arndt University Greifswald, Walther Rathenau Str. 49a, 17489 Greifswald, Germany. kramer@uni-greifswald.de
Galabov
A S
AS
Sattar
S A
SA
Döhner
L
L
Pivert
A
A
Payan
C
C
Wolff
M H
MH
Yilmaz
A
A
Steinmann
J
J
D000433:1-Propanol / Q000032:analysis; D000328:Adult; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D000440:Butanols / Q000032:analysis; D017927:Calicivirus, Feline / Q000187:drug effects; D002415:Cats; D002417:Cattle; D002460:Cell Line; D004202:Disinfectants / Q000737:chemistry / Q000494:pharmacology*; D004357:Drug Synergism; D000431:Ethanol / Q000032:analysis*; D005260:Female; D005385:Fingers / Q000821:virology; D006235:Hand Disinfection / Q000379:methods*; D006801:Humans; D008297:Male; D010756:Phosphoric Acids / Q000032:analysis; D017955:Poliovirus / Q000187:drug effects; D019946:Propylene Glycol / Q000032:analysis; D014780:Viruses / Q000187:drug effects*
D000998:Antiviral Agents; D000440:Butanols; D004202:Disinfectants; D010756:Phosphoric Acids; D000431:Ethanol; D019946:Propylene Glycol; D000433:1-Propanol; C030242:phosphoric acid