title: Antioxidant activity and cytoprotective effect of kappa-carrageenan oligosaccharides and their different derivatives.
abstract: Antioxidant activity of kappa-carrageenan oligosaccharides (OM) and their chemical modification derivatives was investigated employing various established in vitro systems, such as reducing power, iron ion chelation, and total antioxidant activity using beta-carotene-linoleic acid system. The oversulfated (SD), lowly (LAD), and highly acetylated derivatives (HAD) in reducing power assay, the phosphorylated derivative (PD) in metal chelating assay, and oversulfated and phosphorylated derivatives in total antioxidant activity assay exhibited antioxidant activity higher than that of carrageenan oligosaccharides. The results indicated that the chemical modification of carrageenan oligosaccharides can enhance their antioxidant activity in vitro. The protective effects of the carrageenan oligosaccharides and their chemically modified derivatives against H(2)O(2) and UVA (long-wave ultraviolet radiation) induced oxidative damage on rat thymic lymphocyte were investigated by measuring cell viability via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Thymic lymphocyte exposure to H(2)O(2) and UVA, a marked reduction in cell survival was observed, which was significantly prevented by carrageenan oligosaccharides and their derivatives (preincubated for 2h) at 66.7-2000 microg/mL. But both the carrageenan oligosaccharides and their different derivatives showed the similar protective effects on intracellular level. Taken together, these results suggest that carrageenan oligosaccharides and their derivatives show relevant antioxidant activity both in vitro and in a cell system.
authors.affiliation: Institute of Oceanology, Chinese academy of Sciences, Qingdao 266071, PR China.
authors.forename: Huamao
authors.initials: H
authors.lastname: Yuan
authors.forename: Jinming
authors.initials: J
authors.lastname: Song
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.forename: Xuegang
authors.initials: X
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Xuelu
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002351:Carrageenan / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D019610:Cytoprotection / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D009844:Oligosaccharides; D002351:Carrageenan; D006861:Hydrogen Peroxide