Adaptive cellular protection against UVA-1-induced lipid peroxidation in human dermal fibroblasts shows donor-to-donor variability and is glutathione dependent.
Photo-oxidative stress and subsequent lipid peroxidation (LPO) is one of the major mechanisms of UVA-related skin pathology. The skin's protection system against photo-oxidative stress involves low molecular scavengers as well as highly specialised antioxidant enzymes like glutathione peroxidase (GPX). Against repetitive UVA-1 exposures in vitro it is partly adaptive, as recent studies have shown exemplarily for antioxidant enzymes. We now investigated in vitro by repetitively irradiating human dermal fibroblasts with UVA-1 whether this adaptive response might reflect itself in reduced cellular membrane damage, that is, LPO. Our experiments show that the degree of cellular protection against LPO and the adaptive potential of the cells against a repetitive UVA-1 exposure varies from donor-to-donor and depends highly on glutathione.
Schneider
Lars Alexander
LA
Department of Dermatology and Allergology, University of Ulm, Maienweg 12, 89081 Ulm-Söflingen, Germany.
Dissemond
Joachim
J
Brenneisen
Peter
P
Hainzl
Adelheid
A
Briviba
Karlis
K
Wlaschek
Meinhard
M
Scharffetter-Kochanek
Karin
K
D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002478:Cells, Cultured; D003627:Data Interpretation, Statistical; D020405:Dermis / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D005347:Fibroblasts / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D005978:Glutathione / Q000032:analysis / Q000502:physiology*; D005979:Glutathione Peroxidase / Q000502:physiology; D006801:Humans; D015227:Lipid Peroxidation / Q000528:radiation effects*; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000032:analysis; D012879:Skin Physiological Phenomena; D014466:Ultraviolet Rays
D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D019328:Buthionine Sulfoximine; D005979:Glutathione Peroxidase; D005978:Glutathione