Quantification of the virus-host interaction in human T lymphotropic virus I infection.
BACKGROUND
HTLV-I causes the disabling inflammatory disease HAM/TSP: there is no vaccine, no satisfactory treatment and no means of assessing the risk of disease or prognosis in infected people. Like many immunopathological diseases with a viral etiology the outcome of infection is thought to depend on the virus-host immunology interaction. However the dynamic virus-host interaction is complex and current models of HAM/TSP pathogenesis are conflicting. The CD8+ cell response is thought to be a determinant of both HTLV-I proviral load and disease status but its effects can obscure other factors.


RESULTS
We show here that in the absence of CD8+ cells, CD4+ lymphocytes from HAM/TSP patients expressed HTLV-I protein significantly more readily than lymphocytes from asymptomatic carriers of similar proviral load (P = 0.017). A high rate of viral protein expression was significantly associated with a large increase in the prevalence of HAM/TSP (P = 0.031, 89% of cases correctly classified). Additionally, a high rate of Tax expression and a low CD8+ cell efficiency were independently significantly associated with a high proviral load (P = 0.005, P = 0.003 respectively).


CONCLUSIONS
These results disentangle the complex relationship between immune surveillance, proviral load, inflammatory disease and viral protein expression and indicate that increased protein expression may play an important role in HAM/TSP pathogenesis. This has important implications for therapy since it suggests that interventions should aim to reduce Tax expression rather than proviral load per se.
Asquith
Becca
B
Department of Immunology, Imperial College, London W2 1PG, UK. b.asquith@imperial.ac.uk
Mosley
Angelina J
AJ
Heaps
Adrian
A
Tanaka
Yuetsu
Y
Taylor
Graham P
GP
McLean
Angela R
AR
Bangham
Charles R M
CR
D000328:Adult; D000368:Aged; D018791:CD4 Lymphocyte Count; D002353:Carrier State / Q000821:virology; D016356:Gene Products, tax / Q000032:analysis* / Q000235:genetics; D015490:HTLV-I Infections / Q000276:immunology / Q000821:virology*; D006801:Humans; D008875:Middle Aged; D011533:Proviruses / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019562:Viral Load
D016356:Gene Products, tax; D012333:RNA, Messenger