title: Cerebrospinal fluid proteomics and human immunodeficiency virus dementia: preliminary observations.
abstract: Protein profiling using mass spectrometry may be useful in identifying previously unknown protein markers in human immunodeficiency virus (HIV) dementia and provide insight into disease pathogenesis. Six samples of matched cerebrospinal (CSF) and blood serum from patients with no, mild, and moderate dementia were prepped for biomarker screening by the Ciphergen system. Chips were analyzed in the matrix-assisted laser desorption/ionization (MALDI) mass spectrometer at low mass (700 to 20,000 Da) and at higher mass (5000 to 100,000 Da). In both serum and CSF samples, differences in protein intensity appeared to correlate with degree of dementia. This preliminary study suggests that protein markers of HIV dementia may be detected by MALDI mass spectrometry.
authors.affiliation: Department of Neurology, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0284, USA. jrbneuro@uky.edu
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Berger
authors.forename: Malcolm
authors.initials: M
authors.lastname: Avison
authors.forename: Yunanan
authors.initials: Y
authors.lastname: Mootoor
authors.forename: Carol
authors.initials: C
authors.lastname: Beach
mesh_terms: D015526:AIDS Dementia Complex / Q000134:cerebrospinal fluid* / Q000503:physiopathology / Q000821:virology; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D001798:Blood Proteins / Q000737:chemistry*; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D006678:HIV / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D040081:Protein Array Analysis / Q000379:methods; D040901:Proteomics
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins