[Study on drug food extract on lung cancer by seropharmacology].
OBJECTIVE
The purpose of the study is to establish a new process which unites the seropharmacology and cytobiological experiments to reflect the exact effects of herb-food extract (HFE) on, lung cancer, and provides some scientific evidence for the actions of anti-cancer by foods.


METHODS
The optimal conditions of the seropharmacology method were explored by an orthogonal experimental design. The effects of HFE on lung cancer cell YTLMC-90 were proceeded by means of three assays as follows: Methyl thiazolyl tetrazolium (MTT) assay, cell double time analysis and colony forming assay.


RESULTS
The method was successfully built up and the best combination was, as to the HFE, giving to the rats 2 times a day (2%), totally lasting 3 days, and adding 10% of the serum as in the culture medium. The next study illustrated that all the HFEs, including 0.5 g/ml, 1.0 g/ml with 2.0 g/ml ones, had inhibitive capabilities on the growth and proliferation of lung cancer cells, specially those of giving 2.0 g/ml in 3 days and 0.5 g/ml in 10 days. And there was no significant difference between the last two.


CONCLUSIONS
Seropharmacology is a useful method to the study of the effects of mixtures combined with cytobiological experiments. The inhibitive ability of HFE on lung cancer cells suggests that it has effective value in cancer prevention.
Li
Ming
M
School of Public Health, Sichuan University, Chengdu 610041, China.
Huang
Cheng-Yu
CY
Wei
Da-Peng
DP
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005502:Food; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051381:Rats; D044967:Serum
D004365:Drugs, Chinese Herbal