title: Identification of microsatellite markers linked to progressive retinal atrophy in American Eskimo Dogs.
abstract: OBJECTIVE
  To identify microsatellite markers linked to progressive retinal atrophy (PRA) in American Eskimo Dogs.
  
  
  METHODS
  Blood samples or buccal epithelial cells from 66 American Eskimo Dogs, including 53 PRA-unaffected and 13 PRA-affected dogs.
  
  
  METHODS
  The genotypes of unaffected and affected dogs were determined by use of microsatellite markers spanning canine chromosome 9 (CFA09). Homozygosity mapping was used to detect linkage between markers and the gene locus for PRA.
  
  
  RESULTS
  Significant allelic association between marker alleles and the gene locus for PRA was detected for GALK1 and TK1, indicating linkage between these markers and the causative gene locus for PRA.
  
  
  CONCLUSIONS
  These data indicate that PRA in American Eskimo Dogs is located on CFA09 and allow for the development of a microsatellite-based test to identify carrier (unaffected) and affected dogs before clinical signs appear.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Moody
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Famula
authors.forename: Ruth C
authors.initials: RC
authors.lastname: Sampson
authors.forename: Keith E
authors.initials: KE
authors.lastname: Murphy
mesh_terms: D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D015785:Eye Diseases, Hereditary / Q000235:genetics / Q000662:veterinary*; D005260:Female; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012164:Retinal Diseases / Q000235:genetics / Q000662:veterinary*
chemical_list: D004247:DNA