Lateral root bridging as a strategy to enhance L-DOPA production in Stizolobium hassjoo hairy root cultures by using a mesh hindrance mist trickling bioreactor.
Stizolobium hassjoo hairy roots exhibited a lateral root bridging behavior, enabling not only root dry weight but enhancement of intracellular L-DOPA content. When a single root tip was exerted a proper hindrance, the primary root growth was inhibited while lateral roots were profusely induced. The hindrance-induced lateral roots from individual primary root could bridge together under appropriate inoculation densities, leading to high density hairy root cultures producing secondary metabolites. In the present paper, a novel bioreactor was proposed based on a strategy of lateral root bridging by utilizing mesh as a hindrance, called "mesh hindrance mist trickling bioreactor (MHMTB)". Significant improvements of dry weight and L-DOPA production by using MHMTB were 1.8 and 2.2-folds, respectively, higher than those in the control run without the mesh hindrance within the root bed.
Sung
Lin-Shiang
LS
Department of Chemical Engineering, National Taiwan University, Taipei, 10617, Taiwan.
Huang
Shih-Yow
SY
D019149:Bioreactors; D007887:Fabaceae / Q000737:chemistry / Q000254:growth & development*; D007980:Levodopa / Q000096:biosynthesis* / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry / Q000254:growth & development*
D007980:Levodopa