Heterogeneous t(4;11) fusion transcripts in two infants with acute lymphoblastic leukemia.
An RT-PCR assay detected the t(4;11) translocation in two infants with acute lymphoblastic leukemia (ALL). Case P76 was a 10-month-old, female infant, who presented with a WBC of 137.4 x 10(9)/l and a pre-pre-B ALL immunophenotype. Case P120 was a 6-month-old female infant, with a WBC > 615 x 10(9)/l and a pre-pre-B ALL immunophenotype. RT-PCR of cDNA from both these cases generated a 656 bp and a 542 bp respectively, which sequencing confirmed as t(4;11) fusion transcripts. The primers and conditions selected for this assay are compatible with a one-step multiplex PCR for the main translocations in childhood ALL.
Gill
Harvindar Kaur
HK
Allergy & Immunology Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia. gillhk@imr.gov.my
Ten
Sew Keoh
SK
Dhaliwal
Jasbir Singh
JS
Moore
Sarah
S
Hassan
Roshida
R
Karim
Faraiza Abdul
FA
Zakaria
Zubaidah
Z
Murad
Shahnaz
S
Mohamed
Mahfuzah
M
Ibrahim
Hishamshah Mohamad
HM
Rahman
Eni Juraida Abdul
EJ
D001483:Base Sequence; D001854:Bone Marrow Cells / Q000473:pathology; D002880:Chromosomes, Human, Pair 11; D002894:Chromosomes, Human, Pair 4; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic
D004273:DNA, Neoplasm