title: GATA-3 expression in the development of anterior chamber associated immune deviation.
abstract: BACKGROUND
  Anterior chamber associated immune deviation (ACAID) is characterized by a Th2 cell response. GATA-3 has been shown to be necessary for the activation of Th2 cells. This study was designed to examine the expression of GATA-3 in the development of ACAID.
  
  
  METHODS
  ACAID was induced by injection of 50 microg interphotoreceptor retinoid binding protein (IRBP) into the anterior chamber (AC) of Wistar rats. Delayed-type hypersensitivity (DTH) was evaluated on day 3, 7, 14, 21, 28 after IRBP inoculation. GATA-3 expression was detected using immunohistochemical staining. The expression of GATA-3 mRNA at different time points after AC injection of IRBP was assayed by reverse transcriptase polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  A significant DTH reaction was observed in Wistar rats on day 3 and 5 after IRBP inoculation. The DTH reaction was decreased 7 days after IRBP inoculation. GATA-3 expression was weak at both mRNA and protein levels in the normal spleen, but was significantly increased on day 5, 7, 14, and 21 after AC injection of IRBP.
  
  
  CONCLUSIONS
  The expression of GATA-3 is increased during ACAID, suggesting that GATA-3 may be involved in the development of ACAID.
authors.affiliation: Uveitis Study Center, Sun Yat-sen University, Guangzhou 510060, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Fu
authors.forename: Pei-zeng
authors.initials: PZ
authors.lastname: Yang
authors.forename: Xiang-kun
authors.initials: XK
authors.lastname: Huang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huang
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Zhou
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Hua-hong
authors.initials: HH
authors.lastname: Zhong
authors.forename: Xuan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D005136:Eye Proteins / Q000276:immunology; D005260:Female; D050990:GATA3 Transcription Factor / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012177:Retinol-Binding Proteins / Q000276:immunology; D013154:Spleen / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology*; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D005136:Eye Proteins; D050990:GATA3 Transcription Factor; D012333:RNA, Messenger; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial