Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens.
Severe acute respiratory syndrome (SARS) is a recently discovered viral disease, characterized by fever, cough, acute fibrinous pneumonia and high infectivity. Specific pathogen-free (SPF) chickens were immunized with inactivated SARS coronavirus and their eggs were harvested at regular intervals. Yolk immunoglobulin (IgY) was extracted using the water dilution method, followed by further purification on a Sephadex G-75 column. SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and neutralization test results showed that the IgY obtained was of a high purity and had a strong reactive activity with a neutralization titer of 1:640. Lyophilization and stability tests showed that lyophilized anti-SARS coronavirus IgY had promising physical properties, with no significant reduction in reactive activity and good thermal stability. All these data suggest that the anti-SARS coronavirus IgY could be a new useful biological product for specific antiviral therapy against SARS.
Fu
Chao-Yang
CY
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Huang
He
H
Wang
Xiao-Mei
XM
Liu
Yong-Gang
YG
Wang
Zhi-Guo
ZG
Cui
Shang-Jin
SJ
Gao
Hong-Lei
HL
Li
Zan
Z
Li
Jing-Peng
JP
Kong
Xian-Gang
XG
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens; D004530:Egg Yolk / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005612:Freeze Drying; D007114:Immunization; D007136:Immunoglobulins / Q000276:immunology* / Q000302:isolation & purification; D009500:Neutralization Tests; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000517:prevention & control* / Q000662:veterinary / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors
D000914:Antibodies, Viral; C016512:IgY; D007136:Immunoglobulins