Gene discovery in neuropharmacological and behavioral studies using Affymetrix microarray data.
We describe methods and software tools for doing data analysis based on Affymetrix microarray data, emphasizing often neglected issues. In our experience with neuroscience studies, experimental design and quality assessment are vital. We also describe in detail the pre-processing methods we have found useful for Affymetrix data. Finally, we summarize the statistical literature and describe some pitfalls in the post-processing analysis.
Reimers
Mark
M
Laboratory of Molecular Pharmacology, NCI, National Institutes of Health, Bethesda, USA. reimersm@mail.nih.gov
Heilig
Markus
M
Sommer
Wolfgang H
WH
D000818:Animals; D001921:Brain / Q000502:physiology*; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012984:Software