[Simultaneous determination of the four effective components in Huaijiao pill by HPLC].
OBJECTIVE
To develop an HPLC method for determination of the four effective components (genistin; rutin; quercetin; genistein) in Huaijiao pill.


METHODS
The chromatographic separation was performed on a Shim-packODS (4.6 mm x 150 mm, 5.0 microm) eluted with a mobile phase of MeOH-H2O-HAc (40:60:0.25). The detection wavelength was set at 256 nm and column temperature was set at 30 degrees C .


RESULTS
Nice linear relation between the peak area and injected amount exists when the amount is within 0.059-0.352 microg for genistin, within 0.435-2.610 microg for rutin, within 0.020-0.121 microg for quercetin and within 0.053-0.319 microg for genistein. The correlation coefficient of each component is 0.999 6, 0.998 2, 0.998 9 and 0.999 9 respectively. The average recoveries of the four components are from 98.7% to 100.2%. The RSD of each group are 1.21%, 1.36%, 0.47% and 1.54% (n = 5).


CONCLUSIONS
The method was accurate, repeatable and suitable to determine four effective components in Huaijiao pill. It can be use for quality control of Huaijiao pill.
Bian
Qing-Quan
QQ
Mianyang Normal University, Mianyang 621000, China. Bian-55@sohu.com
Yang
Zhen-Ping
ZP
Liu
Jia-Qin
JQ
Liu
Si-Man
SM
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D019833:Genistein / Q000032:analysis*; D007529:Isoflavones / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000032:analysis*; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis*; D029909:Sophora / Q000737:chemistry*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C040641:genistin; D012431:Rutin; D011794:Quercetin; D019833:Genistein