Salt is necessary for nucleosomal DNA fragmentation induced by caspase.
For nucleosomal DNA fragmentation, one of the hallmarks of apoptosis, activated caspase, an apoptosis specific cysteine protease, is required to cleave ICAD/DFF45 that releases its complexed DNase, CAD/DFF40. The protein complex is located predominantly in the nuclei. Inconsistently, caspase alone cannot induce DNA fragmentation in the isolated nuclei without the addition of a cell extract or purified CAD/DFF40. In this study, however, it is demonstrated that under selected conditions with 50-75 mM: KCl or NaCl, caspase-3 and-7 can induce DNA fragmentation without the additional factor(s).
Kim
Bo-Eun
BE
Research Center for Proteineous Materials (RCPM) and Department of Bio-Materials Engineering, Chosun University, 375 Seosuk-Dong, Dong-Ku, Gwangju 501-759, Republic of Korea.
Lee
Yun-Hee
YH
Seong
Changhyun
C
Shin
Song Yub
SY
Lee
Jung Sup
JS
Cho
Byung Yun
BY
Park
Il-Seon
IS
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins; D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D053148:Caspase 3; D061945:Caspase Inhibitors; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism; D002850:Chromatography, Gel; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects*; D003851:Deoxyribonucleases / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D009154:Mutation / Q000235:genetics; D009707:Nucleosomes / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D000075223:Poly-ADP-Ribose Binding Proteins; D011189:Potassium Chloride / Q000494:pharmacology; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012492:Salts / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology
D051017:Apoptosis Regulatory Proteins; D061945:Caspase Inhibitors; D002843:Chromatin; D015853:Cysteine Proteinase Inhibitors; D009707:Nucleosomes; D009842:Oligopeptides; D000075223:Poly-ADP-Ribose Binding Proteins; D011506:Proteins; D012492:Salts; C108637:aspartyl-glutamyl-valyl-aspartal; C109664:caspase-activated DNase inhibitor; C118632:tyrosyl-valyl-alanyl-aspartal; D001312:Aurintricarboxylic Acid; D012965:Sodium Chloride; D011189:Potassium Chloride; D003851:Deoxyribonucleases; C491882:Dffb protein, rat; C505407:CASP3 protein, human; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases