title: Colonisation of Pinus halepensis roots by Pseudomonas fluorescens and interaction with the ectomycorrhizal fungus Suillus granulatus.
abstract: Colonisation of Pinus halepensis roots by GFP-tagged Pseudomonas fluorescens Aur6 was monitored by epifluorescence microscopy and dilution plating. Aur6-GFP was able to colonise and proliferate on P. halepensis roots. Co-inoculation with the ectomycorrhizal fungus Suillus granulatus did not affect the bacterial colonisation pattern whereas it had an effect on bacterial density. Bacterial counts increased during the first 20 days of seedling growth, irrespective of seedlings being mycorrhizal or not. After 40 days, bacterial density significantly decreased and bacteria concentrated on the upper two-thirds of the pine root. The presence of S. granulatus significantly stimulated survival of bacteria in the root elongation zone where fungal colonisation was higher. The number of mycorrhizas formed by S. granulatus was not affected by co-inoculation with Aur6-GFP. Neither Aur6-GFP nor S. granulatus stimulated P. halepensis development when inoculated alone, but a synergistic effect was observed on seedling growth when bacteria and fungus were co-inoculated.
authors.affiliation: Department of Plant Physiology and Biochemistry, Centro de Ciencias Medioambientales, CSIC, Serrano, 115-bis, 28006 Madrid, Spain. ana.rincon@ccma.csic.es
authors.forename: Ana
authors.initials: A
authors.lastname: Rincón
authors.forename: Beatriz
authors.initials: B
authors.lastname: Ruiz-Díez
authors.forename: Sonia
authors.initials: S
authors.lastname: García-Fraile
authors.forename: José Antonio Lucas
authors.initials: JA
authors.lastname: García
authors.forename: Mercedes
authors.initials: M
authors.lastname: Fernández-Pascual
authors.forename: José J
authors.initials: JJ
authors.lastname: Pueyo
authors.forename: María R
authors.initials: MR
authors.lastname: de Felipe
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001487:Basidiomycota / Q000254:growth & development*; D015169:Colony Count, Microbial; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D038821:Mycorrhizae; D028223:Pinus / Q000254:growth & development / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*; D011551:Pseudomonas fluorescens / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D049452:Green Fluorescent Proteins