PCR protocol for specific identification of Candida nivariensis, a recently described pathogenic yeast.
Candida nivariensis is a recently described pathogenic yeast closely related to Candida glabrata. We developed a specific set of oligonucleotide primers based on the internal transcribed spacer regions of the rRNA gene for the rapid identification of C. nivariensis. PCR with these primers amplified a 206-bp amplicon from C. nivariensis.
Alcoba-Flórez
Julia
J
Unidad de Investigación, Hospital Universitario Nuestra Señora de Candelaria, Ctra. del Rosario s/n, 38010 Santa Cruz de Tenerife, Canary Islands, Spain.
Arévalo
María del Pilar
Mdel P
González-Paredes
F Javier
FJ
Cano
Josep
J
Guarro
Josep
J
Pérez-Roth
Eduardo
E
Méndez-Alvarez
Sebastián
S
D002175:Candida / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D002177:Candidiasis / Q000382:microbiology*; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D006801:Humans; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
D017931:DNA Primers; D004271:DNA, Fungal