Useful applications of DOSY experiments for the study of mushroom polysaccharides.
DOSY analysis has been performed on different mushroom extracts and fractions with the aim to describe a general method for the study of high molecular weight polysaccharides. These NMR experiments can be exploited to monitor the fractionation pathways performed on crude extracts in order to isolate polysaccharides. DOSY can also rapidly verify the purity of the isolated compounds, as well as evaluate their molecular size. In spite of the complexity of DOSY spectra of mixtures, this NMR technique seems to be a valid analytical tool that could be adopted as a routine method for the study of polysaccharides from different sources.
Politi
Matteo
M
Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.
Groves
Patrick
P
Chávez
M Isabel
MI
Cañada
F Javier
FJ
Jiménez-Barbero
Jesús
J
D000363:Agaricales / Q000737:chemistry*; D000364:Agaricus / Q000737:chemistry*; D004058:Diffusion; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
D011134:Polysaccharides