Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies.
This study was conducted to determine the detection limit of an optimized DNA microarray assay for detection and species identification of chlamydiae. Examination of dilution series of a plasmid standard carrying the target sequence from Chlamydia trachomatis and genomic DNA of this organism revealed that a single PCR-amplifiable target copy was sufficient to obtain a specific hybridization pattern. This performance renders the test suitable for routine testing of clinical samples.
Ehricht
Ralf
R
Clondiag Chip Technologies GmbH, Loebstedter Str. 105, 07743 Jena, Germany.
Slickers
Peter
P
Goellner
Stefanie
S
Hotzel
Helmut
H
Sachse
Konrad
K
D002692:Chlamydia trachomatis / Q000235:genetics; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity