title: Immobilization of glucose oxidase in conducting graft copolymers and determination of glucose amount in orange juices with enzyme electrodes.
abstract: Glucose oxidase was immobilized in conducting copolymers of three different types of poly(methyl methacrylate-co-thienyl methacrylate). Immobilization of enzyme was carried out by the entrapment in conducting polymers during electrochemical polymerization of pyrrole on the copolymer electrodes. Maximum reaction rate, Michaelis-Menten constants, temperature, pH and operational stabilities were determined for immobilized enzyme. The amount of glucose in orange juices of Turkey was investigated by using enzyme electrodes.
authors.affiliation: Department of Chemistry, Middle East Technical University, 06531 Ankara, Turkey.
authors.forename: Hüseyin Bekir
authors.initials: HB
authors.lastname: Yildiz
authors.forename: Senem
authors.initials: S
authors.lastname: Kiralp
authors.forename: Levent
authors.initials: L
authors.lastname: Toppare
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Yağci
mesh_terms: D001628:Beverages / Q000032:analysis; D015374:Biosensing Techniques; D032084:Citrus sinensis / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D019904:Polymethyl Methacrylate; D005949:Glucose Oxidase; D005947:Glucose