Stimulation of catecholamine synthesis through unique estrogen receptors in the bovine adrenomedullary plasma membrane by 17beta-estradiol.
Incubation of cultured bovine adrenal medullary cells with 17beta-estradiol (E(2)) (0.3-100nM) or membrane-impermeable E(2)-bovine serum albumin (100nM) acutely increased (14)C-catecholamine synthesis from [(14)C]tyrosine. The stimulatory effect of E(2) was not inhibited by ICI182,780, a nuclear estrogen receptor inhibitor. E(2) also increased tyrosine hydroxylase activity and p44/42MAPK phosphorylation, the former of which was attenuated by U0126, an inhibitor of p44/42MAPK kinase. The plasma membrane isolated from the gland showed two classes of specific binding sites of [(3)H]E(2) with apparent K(d)s of 3.2 and 106nM, and B(max)s of 0.44 and 8.5pmol/mg protein, respectively. The high-affinity binding of [(3)H]E(2) was most strongly inhibited by E(2) and phytoestrogens, and to lesser extents by other steroid hormones, while it was enhanced by ICI182,780 and environmental estrogenic pollutants. These findings suggest that E(2) acutely stimulates catecholamine synthesis via activation of p44/42MAPK through unique estrogen receptors in the plasma membrane of bovine adrenal medulla.
Yanagihara
Nobuyuki
N
Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan. yanagin@med.uoeh-u.ac.jp
Liu
Minhui
M
Toyohira
Yumiko
Y
Tsutsui
Masato
M
Ueno
Susumu
S
Shinohara
Yuko
Y
Takahashi
Kojiro
K
Tanaka
Kazumi
K
D000313:Adrenal Medulla / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000096:biosynthesis*; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism*; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism
D002395:Catecholamines; D011960:Receptors, Estrogen; D004958:Estradiol; D014446:Tyrosine 3-Monooxygenase