title: [Study on chemical constituents from Incarvillea arguta and their accelerating PC-12 cell differentiation].
abstract: OBJECTIVE
  To study chemical constituents of Incarvillea arguta and their accelerating PC-12 cell differentiation.
  
  
  METHODS
  The constituents were isolated and repeatedly purified on silica gel column chromatography, and were identified on the basis of physicochemical and spectroscopic analysis. The neurotrophic activity of different portion and all purified compounds from I. arguta was determined on the model of PC-12 cell.
  
  
  RESULTS
  Five compounds were isolated from BuOH portion of alcohol extraction of I. arguta. Their structures were identified as plantarenaloside (I), 5-hydroxy-4', 6 7-trimethoxy-flavone (II), 4', 5-dihydroxy-6, 7-dimethoxyflavone (III), 4', 5-dihydroxy-7-methoxyflavone (IV), 5-dydroxy-4', 7-dimethoxyflavone (V).
  
  
  CONCLUSIONS
  Compound I is isolated from the plant for the first time and it has neurotrophic activity for PC-12 cell. Compounds II approximately V are isolated from the genus Incarvillea for the first time.
authors.affiliation: The Key laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
authors.forename: Zheng-wen
authors.initials: ZW
authors.lastname: Yu
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Zhu
authors.forename: Xiao-sheng
authors.initials: XS
authors.lastname: Yang
authors.forename: Qian-yun
authors.initials: QY
authors.lastname: Sun
authors.forename: Xiao-jiang
authors.initials: XJ
authors.lastname: Hao
mesh_terms: D000818:Animals; D047310:Apigenin / Q000302:isolation & purification* / Q000494:pharmacology; D029402:Bignoniaceae / Q000737:chemistry*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D047309:Flavones / Q000302:isolation & purification* / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats
chemical_list: D047309:Flavones; C014049:salvigenin; C044998:apigenin dimethylether; C014568:genkwanin; C007072:cirsimaritin; D047310:Apigenin