[Rapid determination of optimal time for peripheral blood stem cell harvest by detecting hematopoietor progenitor cell during mobilization].
OBJECTIVE
To develop a simple method to rapidly determine the optimal time of perpheral blood stem cell harvest.


METHODS
We selected 5 healthy donors mobilized with granulocyte colony-stimulating factor (G-CSF) in allogenic peripheral blood stem cell transplantation (allo-PBSCT) to continuously monitor the variety of hematopoietic stem/progenitor cells (HSC/HPC) in the peripheral blood. HPC in the peripheral blood was detected by Sysmex XE-2100 fully automated hematology analyzer with an immature myeloid information channel and the CD34+ cells and colony-forming unit-granulocyte-macrophage (CFU-GM) were determined by flow cytometry and colony culture during mobilization.


RESULTS
HPC was positively correlated with the CD34+ cells or CFU-GM in the peripheral blood (r = 0.82 or r = 0.85, respectively). HPC, CD34+ cells, and CFU-GM in allo-PBSCT donors obviously increased on the 4th day after the mobilization, and their peaks were on the 5th day after the mobilization. But HPC varied more than CD34+ cells and CFU-GM did.


CONCLUSIONS
Sysmex XE-2100 can rapidly monitor the variety of HSC/HPC in allo-PBSCT donors mobilizated with G-CSF, and can provide a simple and economic method to determine the optimal time of peripheral blood stem cell harvest.
Guo
Jing-Jing
JJ
Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, China. cscy1168@sina.com
Wu
Si-Yun
SY
Chen
Yuan-Lin
YL
D000328:Adult; D002452:Cell Count; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D011994:Recombinant Proteins; D013997:Time Factors
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor