title: Increased mitochondrial palmitoylcarnitine/carnitine countertransport by flavone causes oxidative stress and apoptosis in colon cancer cells.
abstract: Cancer cell metabolism is characterized by limited oxidative phosphorylation in order to minimize oxidative stress. We have previously shown that the flavonoid flavone in HT-29 colon cancer cells increases the uptake of pyruvate or lactate into mitochondria, which is followed by an increase in O(2) (-.). production that finally leads to apoptosis. Similarly, a supply of palmitoylcarnitine in combination with carnitine induces apoptosis in HT-29 cells by increasing the mitochondrial respiration rate. Here we show that flavone-induced apoptosis is increased more than twofold in the presence of palmitoylcarnitine due to increased mitochondrial fatty acid transport and the subsequent metabolic generation of O(2) (-.) in mitochondria is the initiating factor for the execution of apoptosis.
authors.affiliation: Molecular Nutrition Unit, Department of Food and Nutrition, Technical University of Munich, Am Forum 5, 85350 Freising, Germany. uwenzel@wzw.tum.de
authors.forename: U
authors.initials: U
authors.lastname: Wenzel
authors.forename: A
authors.initials: A
authors.lastname: Nickel
authors.forename: H
authors.initials: H
authors.lastname: Daniel
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002331:Carnitine / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D047309:Flavones / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D019073:HT29 Cells; D006801:Humans; D008928:Mitochondria / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010172:Palmitoylcarnitine / Q000378:metabolism / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism
chemical_list: D047309:Flavones; D013481:Superoxides; D010172:Palmitoylcarnitine; D002331:Carnitine