Sensitivity of mitochondria isolated from liver and kidney of rat and bovine to lipid peroxidation: a comparative study of light emission and fatty acid profiles.
Much work has been carried out on non-enzymatic-induced lipid peroxidation of mitochondria obtained from different tissues of monogastric animals, but little information is available about this process in poligastric animals. Studies were carried out to determine the sensitivity of mitochondria isolated from liver and kidney of rat and bovine to lipid peroxidation (ascorbate-Fe2+ dependent) by comparison of light emission and fatty acid profiles. Mitochondria from both species were susceptible to lipid peroxidation. Measurements of chemiluminescence indicate that the lipid peroxidation process was more effective in mitochondria from rat liver than in the organelle obtained from bovine, whereas changes were not observed in mitochondria from rat and bovine kidney. The fatty acid composition of total lipids isolated from liver and kidney mitochondria of both species was substantially modified when subjected to non-enzymatic lipid peroxidation with a decrease of arachidonic and docosahexaenoic acids. The polyunsaturated fatty acid (PUFA) composition was higher in mitochondria obtained from rat liver (43.11+/- 4.16) than in bovine (15.78 +/- 0.76). As a consequence, the unsaturation index (UI), was higher in mitochondria of rat liver than in bovine. Nevertheless, the PUFA composition of kidney mitochondria from both species was similar; therefore, statistically significant differences in the UI were not observed. The results suggest that mainly the PUFAs present in hepatic and kidney mitochondria were sensitive to oxidative damage. The lipid peroxidation process was more effective in rat liver mitochondria than in bovine.
Gavazza
Mariana
M
Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CC 296, B1900 AVW, La Plata, Argentina.
Marmunti
Mónica
M
Catalá
Angel
A
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005231:Fatty Acids, Unsaturated; D007668:Kidney / Q000166:cytology*; D015227:Lipid Peroxidation; D008099:Liver / Q000166:cytology*; D049449:Luminescence; D008928:Mitochondria / Q000378:metabolism*; D051381:Rats
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D001205:Ascorbic Acid