title: c-kit Expression and mutations in peripheral T cell lymphomas, except for extra-nodal NK/T cell lymphomas.
abstract: The present study evaluated the expression and mutations of c-kit in peripheral T-cell lymphomas (PTCLs), except for extra-nodal NK/T cell lymphomas, as a potential target for treatment with imatinib mesylate. Fifty-two patients diagnosed with PTCLs (peripheral T-cell lymhoma, unspecified, 38 cases; angioimmunoblastic T-cell, 7 cases; anaplastic large cell, 7 cases) were enrolled. The immunohistochemistry was performed using standard procedures with anti-c-kit monoclonal IgG, while the c-kit mutations were analysed on paraffin-embedded specimens using PCR-single-stranded conformational polymorphism followed by direct DNA sequencing. The median age of the patients was 52 years (19 to approximately 75 years) with a male-to-female ratio of 69%:31%. Weak expression of c-kit was found in 16 (30.8%) patients, while only 3 (5.8%) patients exhibited mutations in exon 11 or exon 13. The c-kit mutations in exon 11 occurred at codon 558 (AAG --> TAG; Lys --> Stop) and at codon 571 (CTA --> ATA; Leu --> Ile), respectively, while the mutation in exon 13 occurred at codon 634 (CGG --> CGA; Arg --> Arg). The current study only found c-kit mutations in a few patients with PTCLs, except for extra-nodal NK/T cell lymphomas. Therefore, c-kit would not seem to be a good target for a new therapeutic approach to PTCLs.
authors.affiliation: Department of Hematology/Oncology, Kyungpook National University Hospital, Daegu, Korea.
authors.forename: Yee Soo
authors.initials: YS
authors.lastname: Choe
authors.forename: Jong Gwang
authors.initials: JG
authors.lastname: Kim
authors.forename: Sang Kyun
authors.initials: SK
authors.lastname: Sohn
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Kim
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Baek
authors.forename: Kyu Bo
authors.initials: KB
authors.lastname: Lee
authors.forename: Young Rok
authors.initials: YR
authors.lastname: Do
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Kwon
authors.forename: Hong Suk
authors.initials: HS
authors.lastname: Song
authors.forename: Myung Hoon
authors.initials: MH
authors.lastname: Lee
authors.forename: Tae In
authors.initials: TI
authors.lastname: Park
mesh_terms: D000328:Adult; D000368:Aged; D005091:Exons; D005260:Female; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007150:Immunohistochemistry; D007694:Killer Cells, Natural / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D016411:Lymphoma, T-Cell, Peripheral / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019009:Proto-Oncogene Proteins c-kit