title: The role of centromere-binding factor 3 (CBF3) in spindle stability, cytokinesis, and kinetochore attachment.
abstract: The spindle midzone is critical for spindle stability and cytokinesis. Chromosomal passenger proteins relocalize from chromosomes to the spindle midzone after anaphase onset. The recent localization of the inner-kinetochore, centromere-binding factor 3 (CBF3) complex to the spindle midzone in budding yeast has led to the discovery of novel functions for this complex in addition to its essential role at kinetochores. In G1/S cells, CBF3 components are detected along dynamic microtubules, where they can "search-and-capture" newly replicated centromeres. During anaphase, CBF3 is transported to the microtubule plus-ends of the spindle midzone. Consistent with this localization, cells containing a mutation in the CBF3 subunit Ndc10p show defects in spindle stability during anaphase. In addition, ndc10-1 cells show defects during cytokinesis, resulting in a defect in cell abscission. These results highlight the importance of midzone-targeted proteins in coordinating mitosis with cell division. Here we discuss these findings and explore the significance of CBF3 transport to microtubule plus-ends at the spindle midzone.
authors.affiliation: Department of Cell Biology, University of North Carolina, Durham, NC 27599, USA.
authors.forename: David
authors.initials: D
authors.lastname: Bouck
authors.forename: Kerry
authors.initials: K
authors.lastname: Bloom
mesh_terms: D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D048749:Cytokinesis / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D018386:Kinetochores / Q000502:physiology*; D008938:Mitosis; D012441:Saccharomyces cerevisiae / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D008941:Spindle Apparatus / Q000737:chemistry / Q000378:metabolism*
chemical_list: C081071:CBF2 protein, S cerevisiae; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins