title: Improved cellular response of osteoblast cells using recombinant human osteopontin protein produced by Escherichia coli.
abstract: Osteopontin is a major non-collagenous bone matrix protein secreted into the mineralizing extracellular matrix by osteoblasts during bone development. Recombinant human osteopontin (hOPN) that includes the Arg-Gly-Asp (RGD) cell recognition site was expressed in Escherichia coli and the purified osteopontin increased cell adhesion, proliferation and differentiation of osteoblast cells (p<0.05).
authors.affiliation: Department of Biochemistry, Inha University College of Medicine, Jung-Gu, 400-712, Incheon, Korea. juhjang@inha.ac.kr
authors.forename: Jun-Hyeog
authors.initials: JH
authors.lastname: Jang
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Kim
mesh_terms: D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D053495:Osteopontin; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000378:metabolism; D012795:Sialoglycoproteins / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011994:Recombinant Proteins; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin