Rapid separation of strychnine and brucine on a dynamically modified poly(dimethylsiloxane) microchip followed by electrochemical detection.
A method has been developed for rapidly separating and detecting strychnine and brucine using a poly(dimethysiloxane) (PDMS) microchip and electrochemical (EC) detection. PDMS microchannels dynamically modified by Brij35 are shown to be more efficient than native ones. The two analytes are well separated within 90 s in 70 mmol/L acetate buffer (pH 5.5) containing 0.01% (v/v) Brij35. Detection limits were found to be 1.0 micromol/L for strychnine and 0.2 micromol/L for brucine at S/N = 3. The method was used to determine trace strychnine and brucine in rat serum, and the results obtained correlate well with those obtained via high-performance liquid chromatography (HPLC).
Zhang
Q L
QL
Key Lab of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, 210093, Nanjing, PR China.
Xu
J J
JJ
Lian
H Z
HZ
Li
X Y
XY
Chen
H Y
HY
D000818:Animals; D002021:Buffers; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D046228:Microarray Analysis / Q000379:methods*; D015394:Molecular Structure; D011092:Polyethylene Glycols; D051381:Rats; D015203:Reproducibility of Results; D013331:Strychnine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D013997:Time Factors
D002021:Buffers; D004129:Dimethylpolysiloxanes; D011092:Polyethylene Glycols; C083806:brucine; D013331:Strychnine; C515901:Brij 35