Analysis of T cell receptor alpha-chain variable region (Valpha) usage and CDR3alpha of T cells infiltrated into lesions of psoriasis patients.
Psoriasis is a common inflammatory skin disease and is considered as T cell-mediated immune response. In this study, we analyzed T cell receptor alpha-chain variable region (TCR Valpha) usage in the lesions of psoriasis patients using 5'-RACE. As the results, Valpha1, -2, -7, -8, -10, -11, -12, and -23 were commonly detected in psoriatic lesions and comparison of expressions of these Valpha types between psoriasis patients and healthy individuals showed that Valpha1, -7, -11, and -12 were highly increased in psoriasis patients than in healthy individuals. Compared with atopy dermatitis patients, the expressions of Valpha1 and Valpha7 were increased in psoriasis patients. Then, to identify CDR3alpha of T cells infiltrated in psoriatic lesions, we examined which type of J gene segment was rearranged with Valpha1 or Valpha7, which the expressions was specifically increased in psoriatic lesions. The result showed that the V-J rearrangements between the examined patients were not equivalent and their frequencies were diverse, however, several common rearrangements such as Valpha1-Jalpha13, -23, -27, or -34 and Valpha7-Jalpha12, -33 were detected. The results in this study might provide the clue to define the characteristics of T cells associated with the pathogenesis of psoriasis.
Hwang
Ha Young
HY
Laboratory of Dermatology-Immunology, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, South Korea.
Kim
Tai-Gyu
TG
Kim
Tae-Yoon
TY
D000595:Amino Acid Sequence; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; D020022:Genetic Predisposition to Disease; D015236:HLA-C Antigens / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D011565:Psoriasis / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000276:immunology / Q000473:pathology; D037182:T-Cell Antigen Receptor Specificity; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology*
D015236:HLA-C Antigens; C491620:HLA-C*06:02 antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta