title: Do computer-assisted, morphometric-derived sperm characteristics reflect DNA status in canine spermatozoa?
abstract: It is widely accepted that sperm morphology is a strong indicator of semen quality. As the sperm head mainly comprises the sperm DNA, it is have been proposed that subtle changes in sperm morphology may be related to abnormal DNA content. Semen from four mongrel dogs was used to investigate DNA quality by means of the sperm chromatin structure assay (SCSA), and for computerized sperm morphometry (CASMA). Each sperm head was measured for nine primary parameters [head area (A), head perimeter (P), head length (L), head width (W), midpiece width (w), midpiece area (a), distance (d) between the major axes of the head and midpiece, angle (theta) of divergence of the midpiece from the head axis] and four parameters of head shape [FUN1, L/W; FUN2, 4piA/P(2); FUN3, (L - W)/(L + W); FUN4, piLW/4A]. Significant differences were found in all CASMA-derived parameters among dogs (p < 0.001). Linear regression models including sperm head shape factors 1, 3 and 4 predicted the extent of DNA denaturation (p < 0.001). We conclude that the CASMA analysis can be considered a powerful tool to improve the spermiogram.
authors.affiliation: Section of Reproduction and Obstetrics, Department of Herd Health and Medicine, Veterinary Teaching Hospital, Avd de la Universidad s/n, 10071 Cáceres, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Núñez-Martinez
authors.forename: J M
authors.initials: JM
authors.lastname: Moran
authors.forename: F J
authors.initials: FJ
authors.lastname: Peña
mesh_terms: D000818:Animals; D002843:Chromatin; D004249:DNA Damage / Q000502:physiology; D004285:Dogs / Q000502:physiology*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008297:Male; D008432:Mathematical Computing; D011237:Predictive Value of Tests; D012661:Semen / Q000502:physiology; D013076:Sperm Count / Q000662:veterinary; D013077:Sperm Head / Q000502:physiology* / Q000648:ultrastructure; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D002843:Chromatin