Putative novel photosynthetic reaction centre organizations in marine aerobic anoxygenic photosynthetic bacteria: insights from metagenomics and environmental genomics.
Photosynthetic core complexes of anoxygenic bacteria consist of reaction centres (RCs) surrounded by light-harvesting complexes (LHC). The structural proteins of the RC-LHC1 complex are encoded by the puf-operon. We find diverse operon organizations of puf-operons that reflect structural differences of the core complex in marine aerobic anoxygenic photosynthetic bacteria (AAnP). By analysis of environmental DNA records coming from AAnP bacteria we find several unknown proteins downstream to the pufM, which were assigned as novel PufX proteins. As all known pufX genes belong to Rhodobacter strains which carry out anaerobic photosynthesis, this may be the first observation of a PufX-containing RCs in aerobic anoxygenic photosynthetic bacteria. Phylogenetic analyses of PufM proteins from cultured as well as from uncultured bacteria show that PufM from operons containing putative novel pufX genes are grouped with Rhodobacter and not with Roseobacter strains.
Yutin
Natalya
N
Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Béjà
Oded
O
D000332:Aerobiosis; D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D001426:Bacterial Proteins / Q000235:genetics*; D000077463:Carbamide Peroxide; D004338:Drug Combinations; D005798:Genes, Bacterial; D045342:Light-Harvesting Protein Complexes / Q000235:genetics*; D008386:Marine Biology; D008969:Molecular Sequence Data; D009876:Operon; D010545:Peroxides / Q000378:metabolism; D010788:Photosynthesis; D016415:Sequence Alignment; D014508:Urea / Q000031:analogs & derivatives / Q000378:metabolism
D001426:Bacterial Proteins; D004338:Drug Combinations; D045342:Light-Harvesting Protein Complexes; D010545:Peroxides; D000077463:Carbamide Peroxide; D014508:Urea