Proteomic analysis and extensive protein identification from dry, germinating Arabidopsis seeds and young seedlings.
Proteins accumulated in dry, stratified Arabidopsis seeds or young seedlings, totaled 1100 to 1300 depending on the time of sampling, were analyzed by using immobilized pH gradient 2-DE gel electrophoresis. The molecular identities of 437 polypeptides, encoded by 355 independent genes, were determined by MALDI-TOF or TOF-TOF mass spectrometry. In the sum, 293 were present at all stages and 95 were accumulated during the time of radicle protrusion while another 18 appeared in later stages. Further analysis showed that 226 of the identified polypeptides could be located in different metabolic pathways. Proteins involved in carbohydrate, energy and amino acid metabolism constituted to about 1/4, and those involved in metabolism of vitamins and cofactors constituted for about 3 % of the total signal intensity in gels prepared from 72 h seedlings. Enzymes related to genetic information processing increased very quickly during early imbibition and reached highest level around 30 h of germination.
Fu
Qiang
Q
The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
Wang
Bai-Chen
BC
Jin
Xiang
X
Li
Hong-Bing
HB
Han
Pei
P
Wei
Kai-hua
KH
Zhang
Xue-min
XM
Zhu
Yu-Xian
YX
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D017343:Genes, Plant; D018525:Germination; D006019:Glycolysis; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D036226:Seedlings / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D014815:Vitamins / Q000378:metabolism
D029681:Arabidopsis Proteins; D010455:Peptides; D014815:Vitamins