title: [Phalloidin plays an important role in calcium ionophore A23187-induced human acrosome reaction].
abstract: OBJECTIVE
  To discuss the important role of actin polymerization in calcium ionophore A23187-induced human acrosome reaction and its mechanism.
  
  
  METHODS
  Each spermatozoon specimen was divided into five groups, treated with A23187 3 micromol/L in Group A, Phalloidin 40 micromol/L and A23187 3 micromol/L in Group B, SLO 0.5 U/ml and A23187 3 micromol/L in Group C, SLO 0.5 U/ ml, Phalloidin 40 micromol/L and A23187 3 micromol/L in Group D, and nothing added in Grpup E. Then the percentage of the human acrosome reaction was assessed with Rhodamine-PSA (10 microg/ml).
  
  
  RESULTS
  The difference of the human spermatozoon acrosome reaction was significant (P < 0.01) among the 5 groups with or without SLO, Phalloidin and calcium ionophore A23187 but not between Groups A and B (P > 0.01).
  
  
  CONCLUSIONS
  Phalloidin does not work on the acrosome reaction of intact human spermatozoa, but in an SLO-permeabilized human spermatozoal model, it can obviously decrease the percentage of human spermatozoon acrosome reaction, which indicates that the polymerization of actin plays an important role in the course of human spermatozoon acrosome reaction, and mostly acts on the acrosome inside.
authors.affiliation: Department of Urology, Qingdao People's Hospital, Affiliated Hospital of Qingdao Medical University, Qingdao, Shandong 266003, China. wsmail@ruyi.com
authors.forename: Shuai
authors.initials: S
authors.lastname: Wu
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Wang
authors.forename: Fan-Hui
authors.initials: FH
authors.lastname: Meng
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects*; D000199:Actins / Q000502:physiology; D001426:Bacterial Proteins / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology*; D002478:Cells, Cultured; D006801:Humans; D007476:Ionophores / Q000494:pharmacology*; D008297:Male; D010590:Phalloidine / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013301:Streptolysins / Q000494:pharmacology
chemical_list: D000199:Actins; D001426:Bacterial Proteins; D007476:Ionophores; D013301:Streptolysins; C025964:streptolysin O; D010590:Phalloidine; D000001:Calcimycin