[A three-dimension in vitro model for angiogenesis of hemangioma].
OBJECTIVE
To create a three dimension (3D) in vitro model for angiogenesis of hemangioma.


METHODS
The fragment of hemangioma specimen was embedded in fibrin gel to set up the three-dimension (3D) in vitro model for angiogenesis of hemangioma.


RESULTS
In the model, microvessels grew out from the tissue fragments at the 2nd to 3rd day after culture, and at the 8th to 9th day a compact network of microvessels come into being, then tending to be stationary. The compact network around the tissue fragment was confirmed to be blood vessels by immunohistochemistry and electron microscopy.


CONCLUSIONS
This model helps to study the mechanism of hemangioma angiogenesis and investigate the drugs of anti-angiogenesis.
Jiang
Cheng-Hong
CH
The Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Zhuang
Fu-Lian
FL
Huang
Ba-Rui
BR
Guo
Zhi-Hui
ZH
Xie
Yi-De
YD
Zhou
Ya-Kuan
YK
Wang
Biao
B
Chen
Wen-lie
WL
D002478:Cells, Cultured; D004730:Endothelium, Vascular; D006391:Hemangioma; D006801:Humans; D008955:Models, Cardiovascular; D009389:Neovascularization, Pathologic