Stress induced in heart and other tissues by rat dermal exposure to JP-8 fuel.
Limited information is available regarding the development of systemic organ stress by dermal exposure to JP-8 fuel. In this study, the systemic stress potential of this fuel is evaluated in a rat model subjected to dermal applications of JP-8 for 7 days at 300 microl per day. Tissue histology indicated that JP-8 induces morphological alterations that suggest that tissue stress in the heart is more substantial than stress in the kidney and liver. Immunoblot analysis of tissues revealed increased levels of the inducible heat shock protein 70 (HSP70) in the heart, kidney, and liver after this dermal JP-8 exposure. This exposure also leads to increased levels of heme oxygenase-1 (HO-1/HSP3) in the liver. Additionally during this exposure, a negative regulator of inflammation, IkappaBalpha (inhibitor of NF-kappaB), was increased in the liver, slightly increased in the kidney, and not increased in the heart. Two regions of the rat brain were also examined and HSP70 and IkappaBalpha were increased in the cerebellum but not significantly increased in the cortex. This study indicates dermal JP-8 exposure causes systemic alterations that are associated with cytoprotective activities (e.g., in the liver) as well as potentially toxic mechanisms (heart and kidney).
Larabee
J L
JL
Departments of Biochemistry and Molecular Biology, University of Oklahoma Health Science Center, Oklahoma City, 73104, USA.
Hocker
J R
JR
Lerner
M R
MR
Lightfoot
S A
SA
Cheung
J Y
JY
Brackett
D J
DJ
Gallucci
R M
RM
Hanas
J S
JS
D000279:Administration, Cutaneous; D000818:Animals; D001923:Brain Chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D000076249:Heat Shock Transcription Factors; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D006838:Hydrocarbons / Q000008:administration & dosage / Q000633:toxicity*; D020672:I-kappa B Proteins / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D000072000:NF-KappaB Inhibitor alpha; D010766:Phosphorylation; D051381:Rats; D020318:Rats, Long-Evans; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism
D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D006838:Hydrocarbons; D020672:I-kappa B Proteins; C098607:JP8 aviation fuel; C000606206:Nfkbia protein, rat; D014157:Transcription Factors; D000072000:NF-KappaB Inhibitor alpha; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat