title: Electrophysiologic differentiation of homozygous and heterozygous Abyssinian-crossbred cats with late-onset hereditary retinal degeneration.
abstract: OBJECTIVE
  To develop a method to electrophysiologically differentiate heterozygous-carrier Abyssinian-crossbred cats from homozygous-affected Abyssinian-crossbred cats before clinical onset of inherited rod-cone retinal degeneration.
  
  
  METHODS
  14 back-crossed Abyssinian-crossbred cats of unknown genotype (homozygous or heterozygous) for inherited rod-cone retinal degeneration, 24 age-matched mixed-breed control cats, 6 age-matched heterozygous Abyssinian-crossbred cats, and 6 homozygous Abyssinian cats.
  
  
  METHODS
  Electroretinography (ERG) of heterozygous and homozygous cats revealed differences, especially for scotopic recordings. Frequent ophthalmoscopy and ERG (2 to 5 times; at intervals of 3 to 6 months) of back-crossed cats were performed. Amplitudes and implicit times were analyzed by use of a graphic representation of results. Ratios for amplitudes of the b-waves to amplitudes of the a-waves (b-wave:a-wave) were compared.
  
  
  RESULTS
  8 back-crossed cats had decreased a-wave amplitudes, increased b-wave implicit times, and abnormal ERG waveforms. Values for the b-wave:a-wave for the highest scotopic light intensity were significantly higher for those same 8 cats.
  
  
  CONCLUSIONS
  The 8 back-crossed Abyssinian-crossbred cats with abnormal results developed fundus changes over time consistent with disease. A graphic representation of ERG results can be used to differentiate between genotypes prior to funduscopic changes. Values for the b-wave:a-wave ratio provide confirmation. These ERG analyses may be applied clinically in the diagnosis of retinal degenerations in various species.
  
  
  CONCLUSIONS
  Cats with hereditary rod-cone degeneration may be a useful model for comparative studies in relation to retinitis pigmentosa in humans. Similar evaluations of ERG results could possibly be used for humans with suspected generalized retinal degeneration.
authors.affiliation: Eye Care for Animals, Overland Park, KS 66210, USA.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Hyman
authors.lastname: Vaegan
authors.forename: Bo
authors.initials: B
authors.lastname: Lei
authors.forename: Kristina L
authors.initials: KL
authors.lastname: Narfström
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000235:genetics*; D002415:Cats; D003433:Crosses, Genetic; D004596:Electroretinography / Q000662:veterinary; D015785:Eye Diseases, Hereditary / Q000235:genetics / Q000662:veterinary*; D012162:Retinal Degeneration / Q000235:genetics / Q000662:veterinary*