[A study on the promoting effects of recombinant human epidermal growth factor on skin wound healing in rats].
OBJECTIVE
To observe the effects of recombinant human epidermal growth factor (rhEGF) on skin wound healing.


METHODS
The dorsal trauma model of rats was used. A total of 68 dorsal wounds in 34 rats were created and divided into the rhEGF group and the isotonic saline group according to self-control. The process of wound healing was observed and a mean healing time was calculated. Furthermore, the dynamic analysis was performed at different times on wound OHP contents, ratio of collagen I/III and cell DNA cycle.


RESULTS
The wound healing was accelerated obviously in all wounds treated with rhEGF. The mean time of wound healing of the two groups was (17.2 +/- 1.3) days and (20.5 +/- 1.6) days respectively (P < 0.01). In the rhEGF group, the new granulation tissue was more and the re-epithelialization was faster than that of the saline group. External rhEGF increased OHP content, reduced collagen I/II ratio and accelerated cell DNA replication.


CONCLUSIONS
External rhEGF can shorten wound healing time, increase granulation tissue and OHP contents, reduce collagen I/III ratio and accelerate cell DNA replication, thus obviously promoting skin wound repair.
Zheng
Jun
J
Department of Burn and Plastic Surgery, Xiang Ya Hospital, Central South University, Hunan Changsha 410008, China.
Huang
Xiao-Yuan
XY
Wei
Xing
X
D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology*; D012867:Skin / Q000293:injuries*; D014945:Wound Healing / Q000187:drug effects*
D011994:Recombinant Proteins; D004815:Epidermal Growth Factor