Antitumor effect of double immunization of mice with mucin 1 and its coding DNA.
OBJECTIVE
To evaluate the antitumor effect of mouse immunization with human mucin 1 gene (muc 1) DNA plasmids combined with simultaneous injections of human mucin 1 (MUC1) protein.


METHODS
MUC1 DNA was cloned in pBK-CMV to prepare DNA plasmids and in pET22b(+) to produce proteins. Three strains of mice, immunized with DNA or DNA plus MUC1, were inoculated with tumor cells obtained from spontaneous tumors. IgG(2a) production, MUC1-specific IFN-delta-producing CD8+ T cells, tumor growth and mouse survival were monitored.


RESULTS
Only immunization with DNA plus proteins induced IgG(2a) and intracellular IFN-delta production by CD8+ T cells in the strains tested. DNA plus protein immunization induced a better mouse survival in comparison with the DNA groups. However, all immunized mice invariably developed tumors.


CONCLUSIONS
Immunization with DNA plus proteins induced a better protection from tumor growth than immunization with naked DNA. However, the efficacy of immunization with MUC1-based antigens remains low.
Mushenkova
Natalya
N
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya St, 16/10, Moscow, 117997, Russian Federation.
Moiseeva
Ekaterina
E
Chaadaeva
Alexandra
A
Den Otter
William
W
Svirshchevskaya
Elena
E
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology* / Q000494:pharmacology; D004247:DNA / Q000235:genetics / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000517:prevention & control / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D018396:Mucin-1 / Q000235:genetics / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology* / Q000494:pharmacology
D019496:Cancer Vaccines; D007074:Immunoglobulin G; D018396:Mucin-1; D019444:Vaccines, DNA; D004247:DNA