[Delayed testing for the diagnosis of fungi in the urines. Evaluation of the BD Vacutainer C&S tubes for the storage of urine samples at room temperature].
The study included 37 urine samples which have been artificially infected with low levels (10(3) CFU/mL) of various fungi strains. We compared the effects of sample storage, up to 48 hours, at room temperature, in a urine evacuated tube containing specific additives with storage at + 4 degrees C, for the same length of time, in a urine evacuated tube without any additives. There have been no differences of results (speed of growth and colony size) between the 2 modes of storage. However, the experience has shown that samples needed a careful mixing before seeding to avoid underdetection of the strains. Based on the study results, the BD Vacutainer C&S tubes are suitable for delayed testing for the diagnosis of urine fungal infection.
Baixench
M T
MT
Laboratoire de parasitologie-mycologie, Groupe hospitalier Cochin-Saint Vincent de Paul, La Roche-Guyon, Paris. marie-therese.baixench@cch.aphp.fr
Al-Sheikh
M
M
Paugam
A
A
D004867:Equipment Design; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D011309:Preservation, Biological / Q000295:instrumentation*; D013997:Time Factors; D014556:Urine / Q000382:microbiology*