Identification of prior candidate genes for Sclerotinia local resistance in Brassica napus using Arabidopsis cDNA microarray and Brassica-Arabidopsis comparative mapping.
Arabidopsis cDNA arrays were used to screen the local-defense-associated genes in oilseed rape (Brassica napus L.) at the challenge of Sclerotinia sclerotiorum. 61 genes with two-fold expression changes were screened out from the local tissue around the necrosis. Among them, 36 unique genes were up-regulated and 25 unique genes were down-regulated. RT-PCR and Northern blot results were consistent with the array results, suggesting Arabidopsis arrays were useful for transcriptional profiling of B. napus genes. Some of these genes were located in the interval of some QTLs for Sclerotinia resistance in B. napus by Brassica-Arabidopsis comparative mapping. These genes may have priority to be pursued for more intensive research.
Liu
Renhu
R
National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070, China.
Zhao
Jianwei
J
Xiao
Yong
Y
Meng
Jinling
J
D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology; D001203:Ascomycota / Q000472:pathogenicity*; D029688:Brassica napus / Q000235:genetics* / Q000382:microbiology; D020869:Gene Expression Profiling; D017343:Genes, Plant; D020411:Oligonucleotide Array Sequence Analysis; D020161:Physical Chromosome Mapping; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D040641:Quantitative Trait Loci / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid