title: Immunogenicity of a multicomponent DNA vaccine against visceral leishmaniasis in dogs.
abstract: Vaccination of dogs, the domestic reservoir of Leishmania chagasi, could not only decrease the burden of canine visceral leishmaniasis (VL), but could also indirectly reduce the incidence of human VL. Intramuscular vaccination of foxhounds with a Leishmania multicomponent (10 antigen) DNA vaccine resulted in antigen-induced lymphoproliferative and IFN-gamma (but not IL-4) responses. This response was not augmented by co-administration of canine IL-12 or GM-CSF DNA adjuvants. The multicomponent DNA vaccine also induced a delayed type hypersensitivity (DTH) response to viable L. donovani promastigotes and led to a reduction of parasite burden in an in vitro intracellular infection model, and in the draining lymph node of dogs early after cutaneous challenge. Thus, the multicomponent DNA vaccine was effective in priming dogs for a parasite-specific type 1 cellular immune response, which was able to restrict parasite growth.
authors.affiliation: Research Service, Department of Veterans Affairs Medical Center, South Texas Veterans Health Care System, 7400 Merton Minter Dr., Mailstop 151, San Antonio 78229-4404, TX, USA.
authors.forename: Omar A
authors.initials: OA
authors.lastname: Saldarriaga
authors.forename: Bruno L
authors.initials: BL
authors.lastname: Travi
authors.forename: Won
authors.initials: W
authors.lastname: Park
authors.forename: Luis E
authors.initials: LE
authors.lastname: Perez
authors.forename: Peter C
authors.initials: PC
authors.lastname: Melby
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology; D016054:DNA, Protozoan / Q000032:analysis; D004197:Disease Reservoirs; D004283:Dog Diseases / Q000517:prevention & control*; D004285:Dogs; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000276:immunology; D006968:Hypersensitivity, Delayed; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000096:biosynthesis; D018314:Leishmania infantum / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000517:prevention & control / Q000662:veterinary*; D008198:Lymph Nodes / Q000469:parasitology; D008213:Lymphocyte Activation; D016133:Polymerase Chain Reaction; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D016052:Protozoan Vaccines; D012333:RNA, Messenger; D019444:Vaccines, DNA; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor