In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate.
Recently, regular and white mineral trioxide aggregate (MTA) are being used in Dentistry as retrofilling materials. Genotoxicity and cytotoxicity tests form an important part of cancer research and risk assessment of potential carcinogens. Thus, the goal of this study was to examine the genotoxicity and cytotoxicity of regular and white MTA in vitro by the single cell gel (comet) assay and trypan blue exclusion test, respectively. Mouse lymphoma cells were exposed to two presentation forms of MTA at final concentrations ranging from 1 to 1,000 microg/mL for 3 h at 37 degrees C. The results showed that both compounds tested did not produce genotoxic effects at all concentrations evaluated. Likewise, no statistically significant differences (p > 0.05) were observed in cytotoxicity. Taken together, our results suggest that regular and white MTA are not genotoxins and are not able to interfere in cellular viability as assessed by single cell gel (comet) assay and trypan blue assay, respectively.
Ribeiro
Daniel Araki
DA
Center for Genotoxin and Carcinogen Evaluation, TOXICAN, Department of Pathology, School of Medicine of Botucatu, São Paulo State University.
Matsumoto
Mariza Akemi
MA
Duarte
Marco Antonio Húngaro
MA
Marques
Mariangela Esther Alencar
ME
Salvadori
Daisy Maria Favero
DM
D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D017610:Calcium Compounds / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects*; D020552:Comet Assay; D004249:DNA Damage; D004338:Drug Combinations; D007940:Leukemia L5178 / Q000235:genetics; D008223:Lymphoma / Q000235:genetics; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D009152:Mutagenicity Tests; D010087:Oxides / Q000633:toxicity*; D012184:Retrograde Obturation; D017640:Silicates / Q000633:toxicity*; D014343:Trypan Blue
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D017640:Silicates; C086631:mineral trioxide aggregate; D014343:Trypan Blue