title: The influence of human plasma used for dentin perfusion on tensile bond strength of different light-curing materials.
abstract: OBJECTIVE
  To evaluate the influence of two perfusion solutions, saline and human plasma, on tensile bond strengths of five different light-curing materials in an in vitro investigation.
  
  
  METHODS
  150 human third molars were used. All teeth were prepared in a special manner allowing the simulation of intrapulpal pressure and dentin perfusion. Dentin specimens with a thickness of 3.5 mm were obtained under standardized conditions. The specimens were randomly assigned to 10 experimental groups. Five groups were perfused with physiologic saline while with the other five with human plasma under constant hydrostatic pressure of 30 cm H2O was used for at least 1 hour. Tensile bond strength of the different dentin bonding agents and composite or compomer materials (A: Syntac/Tetric, B: Prime & Bond NT/Spectrum TPH, C: Solobond Plus/Arabesk, D: Compoglass SCA/Compoglass, E: Dyract PSA/Dyract) was evaluated using an Zwick Universal testing machine 10 minutes after light-curing.
  
  
  RESULTS
  Pairwise comparison showed a significant increase of bond strengths in all human plasma perfused subgroups (P< 0.05; Wilcoxon). The influence of the different dentin adhesives was significant (P< 0.001; ANOVA). The significantly highest values were observed for the composite groups Syntac/Tetric and Solobond Plus/Arabesk (P< 0.05; closed test procedure). The compomer Group D (Compoglass SCA/Compoglass) showed significantly decreased values compared to all other groups (P< 0.05; closed test procedure). Within the limitations of an in vitro study, it can be concluded that the use of human plasma might result in higher tensile bond strength compared to saline.
authors.affiliation: Martin-Luther-University Halle-Wittenberg, University School of Dental Medicine, Department of Operative Dentistry and Periodontology, Grosse Steinstrasse 19, 06108 Halle, Germany. christian.gernhardt@medizin.uni-halle.de
authors.forename: Christian Ralf
authors.initials: CR
authors.lastname: Gernhardt
authors.forename: Hans-Guenter
authors.initials: HG
authors.lastname: Schaller
authors.forename: Andrej Michael
authors.initials: AM
authors.lastname: Kielbassa
mesh_terms: D000704:Analysis of Variance; D003738:Dental Cements / Q000737:chemistry*; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006801:Humans; D008027:Light; D008422:Materials Testing / Q000379:methods; D010949:Plasma; D012965:Sodium Chloride / Q000008:administration & dosage*; D018709:Statistics, Nonparametric; D013718:Tensile Strength / Q000187:drug effects
chemical_list: D003738:Dental Cements; D017220:Dentin-Bonding Agents; D012965:Sodium Chloride