Metallothioneins induction and antioxidative response in aquatic worms Tubifex tubifex (Oligochaeta, Tubificidae) exposed to copper.
Metallothioneins (MTs), are low molecular weight proteins, mainly implicated in metal ion detoxification. Increase in MT contents is considered as a specific biomarker of metal exposure. Recently it has been demonstrated that MTs participate in several cellular functions such as regulation of growth, and antioxidative defences. Tubifex tubifex were exposed to different copper concentrations (50, 100, and 200 microgl(-1)) for 7 and 15 days. MT levels in exposed worms increased significantly (p<0.05) after 7 and 15 days of exposure to different concentrations of copper (maximum +208% for 100 microgl(-1) after 7 days of exposure). Also important perturbation in metal-metallothionein content occurred, along with an increase in total soluble protein content in all treated worms after 7 and 15 days (max. +88.49%). Catalase activities (CAT) in Cu treated-worms were significantly increased, and demonstrated a development of antioxidative defenses. Additionally a reduction of gulathione-S-transferase (GST) was observed in all treated worms after 7 days of exposure to Cu (max. -44.42%). The high induction of MTs observed during T. tubifex exposure to Cu make them potentially useful biomarkers to monitor metal pollution.
Mosleh
Yahia Y
YY
Laboratoire d'Eco-Toxicologie, Unité de Recherche sur la Vigne et le Vin de Champagne UPRES-EA 2069, Faculté des Sciences, Université de Reims Champagne-Ardenne, B.P. 1039, 51687 Reims, cedex 02, France. yahia.mosleh@voila.fr
Paris-Palacios
Séverine
S
Biagianti-Risbourg
Sylvie
S
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers; D002374:Catalase / Q000378:metabolism; D003300:Copper / Q000633:toxicity*; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism*; D009835:Oligochaeta / Q000187:drug effects* / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000633:toxicity
D000975:Antioxidants; D015415:Biomarkers; D011506:Proteins; D014874:Water Pollutants, Chemical; C004203:cadmium-binding protein; C007121:copper thionein; C021267:zinc thionein; D003300:Copper; D008668:Metallothionein; D002374:Catalase; D005980:Glutathione Reductase; D005982:Glutathione Transferase