title: Production of hexenol in a two-enzyme system: kinetic study and modelling.
abstract: In a two-enzyme system, successive action of hydroperoxide lyase from mint and yeast alcohol-dehydrogenase catalyses the conversion of hydroperoxy linolenic acid to hexenol. Kinetic behaviour was investigated separately for each enzyme: a lumped model based on the Michaelis-Menten approach shows the fate of the reactants in the system.
authors.affiliation: Enzyme Engineering Group, National Institute of Applied Science and Technology (INSAT), Tunis, Tunisia. benakachanajla@yahoo.fr
authors.forename: Najla Ben
authors.initials: NB
authors.lastname: Akacha
authors.forename: Olfa
authors.initials: O
authors.lastname: Boubaker
authors.forename: Mohamed
authors.initials: M
authors.lastname: Gargouri
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000446:Aldehyde-Lyases / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism; D000465:Algorithms; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D000441:Hexanols / Q000378:metabolism*; D006591:Hexobarbital / Q000378:metabolism; D008042:Linolenic Acids / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D027541:Mentha / Q000201:enzymology; D015003:Yeasts / Q000201:enzymology
chemical_list: D000447:Aldehydes; D000441:Hexanols; D008042:Linolenic Acids; D008054:Lipid Peroxides; C025998:13-hydroperoxylinolenic acid; C051918:3-hexen-1-ol; D000431:Ethanol; D003577:Cytochrome P-450 Enzyme System; D006591:Hexobarbital; D000426:Alcohol Dehydrogenase; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase