The cold-shock response in cultured mammalian cells: harnessing the response for the improvement of recombinant protein production.
There are a growing number of reports on the sub-physiological temperature culturing (<37 degrees C) of mammalian cells for increased recombinant protein yield, although the effect is variable between cell lines, expression systems, and the product of interest. What is becoming clear is that exposing mammalian cells to sub-physiological temperatures invokes a coordinated cellular response involving modulation of the cell cycle, metabolism, transcription, translation, and the cell cytoskeleton. Opportunities currently exist for further enhancement of the cold-shock effect on recombinant protein production in mammalian cells through advancements in our understanding of the mechanisms involved in the cold-shock response.
Al-Fageeh
Mohamed B
MB
Protein Science Group, Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK.
Marchant
Rosalyn J
RJ
Carden
Martin J
MJ
Smales
C Mark
CM
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002468:Cell Physiological Phenomena; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003080:Cold Temperature; D006224:Cricetinae; D003412:Cricetulus; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D006801:Humans; D051379:Mice; D014176:Protein Biosynthesis / Q000235:genetics / Q000502:physiology; D011994:Recombinant Proteins / Q000096:biosynthesis*
D011994:Recombinant Proteins