[Effect of sinomenine on adjuvant arthritis and its mechanisms].
OBJECTIVE
To discuss the anti-inflammatory mechanism of sinomenine on inflammatory media in joint of adjuvant arthritis rats.


METHODS
Rats were randomly divided into the normal group and the model group, the prednisone group, the small, medium, large of sinomenine group (30, 60, 120 mg x kg(-1)). Except for the rats in the normal group, animals were modeled to adjuvant arthritiswith freund's complete adjuvant. The arthritis index (AI) and the swelling degree of paws were recorded, and the activity of IL-1, TNF and the levels of NO, PGE2 in joint fluids of secondary arthritis were determined.


RESULTS
Compared with the normal group, the activity of IL-1, TNF and the levels of NO, PGE2 in joint fluids of secondary arthritis were increased significantly in the model group (P < 0.05). Compared with the model group, it was shown to exert a dramatic inhibitory effect on secondary reaction of freund's adjuvant arthritis of rats, and the activity of IL-1, TNF and the levels of NO, PGE2 in joint fluids of secondary arthritis were significantly decreased in the sinomenine group (P < 0.05).


CONCLUSIONS
Sinomenine has a remarkable treatment effect on RA. It is via NO to inhibit the activity of cytokines and decrease the level of inflammation mediators, which may be one of its curing RA mechanism.
Yang
De-sen
DS
Department of Pharmacology, Hubei College of Traditional Chinese Medicine, Wuhan 430064, China.
Liu
Fang
F
Zeng
Fan-dian
FD
Chen
Hui
H
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D001169:Arthritis, Experimental / Q000188:drug therapy*; D015232:Dinoprostone / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D007375:Interleukin-1 / Q000378:metabolism; D008297:Male; D009019:Morphinans / Q000627:therapeutic use*; D009569:Nitric Oxide / Q000378:metabolism*; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D032841:Sinomenium / Q000737:chemistry; D013582:Synovial Fluid / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D009019:Morphinans; D009569:Nitric Oxide; C009271:sinomenine; D015232:Dinoprostone