Differential effects of clathrin and actin inhibitors on internalization of Escherichia coli and Salmonella choleraesuis in porcine jejunal Peyer's patches.
Peyer's patches constitute both an inductive immune site and an enteropathogen invasion route. Peyer's patch mucosae from porcine jejunum were mounted in Ussing chambers, and either Salmonella choleraesuis vaccine strain SC-54 or non-pathogenic rodent and porcine Escherichia coli strains contacted the Peyer's patch mucosa for 90 min. Internalized bacteria were quantified by a gentamicin resistance assay. Monodansylcadaverine (300 microM, luminal addition), an inhibitor of clathrin-mediated endocytosis, significantly inhibited internalization of both E. coli strains relative to tissues untreated with the inhibitor; internalization of SC-54 was unaffected. The actin-disrupting agent cytochalasin D (10 microM, luminal addition), inhibited internalization of pig-adapted E. coli but not that of rodent-adapted E. coli or SC-54. Internalization of SC-54 and non-pathogenic E. coli in Peyer's patches appears to occur through different cellular routes.
Green
Benedict T
BT
Department of Veterinary and Biomedical Sciences, University of Minnesota, 1988 Fitch Avenue, St. Paul, MN 55108-6010, USA.
Brown
David R
DR
D000199:Actins / Q000037:antagonists & inhibitors*; D000818:Animals; D002103:Cadaverine / Q000031:analogs & derivatives / Q000494:pharmacology; D002966:Clathrin / Q000037:antagonists & inhibitors*; D015638:Cytochalasin D / Q000494:pharmacology; D004553:Electric Conductivity; D004705:Endocytosis / Q000187:drug effects; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000472:pathogenicity; D005260:Female; D007583:Jejunum; D008297:Male; D010581:Peyer's Patches / Q000187:drug effects / Q000382:microbiology*; D012475:Salmonella / Q000187:drug effects* / Q000472:pathogenicity; D013552:Swine
D000199:Actins; D002966:Clathrin; D015638:Cytochalasin D; C008542:monodansylcadaverine; D002103:Cadaverine