TEL/ARG induces cytoskeletal abnormalities in 293T cells.
We previously identified TEL/ARG as a novel fusion transcript consisting of the oligomerization domain of TEL and the kinase domain of ARG, in a case of acute myeloid leukemia. We report here the existence of an alternatively spliced TEL/ARG transcript lacking part of a F-actin binding domain of ARG, and the phenotype of TEL/ARG expressing 293T cells. In 293T cells, both TEL/ARG forms co-localized with the cellular beta-actin and were associated with a morphologic change of the cells, consisting in cell rounding and detachment from the tissue culture plastic. We identified the Rho inhibitor p190RhoGAP, a critical regulator of cellular adhesion, as a target of the aberrant kinase.
Palmi
Chiara
C
Centro Ricerca Tettamanti, Università di Milano-Bicocca, Ospedale San Gerardo, Monza, Italy.
Fazio
Grazia
G
Cassetti
Arianna
A
Aloisi
Alessandra
A
Villa
Antonello
A
Biondi
Andrea
A
Cazzaniga
Giovanni
G
D017398:Alternative Splicing; D001483:Base Sequence; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism*; D017931:DNA Primers; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation; D050783:Proto-Oncogene Proteins c-ets / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013347:Subcellular Fractions / Q000378:metabolism; D000097764:ETS Translocation Variant 6 Protein
D017931:DNA Primers; D020662:Guanine Nucleotide Exchange Factors; D050783:Proto-Oncogene Proteins c-ets; D012097:Repressor Proteins; D000097764:ETS Translocation Variant 6 Protein; C074560:ARHGAP35 protein, human