Analysis of gene expression induced by microtubule-disrupting agents in HeLa cells using microarray.
Microtubules are important cytoskeletal elements that have been shown to play a major role in many cellular processes because of their mechanical properties and/or their participation in various cell signaling pathways. Nocodazole is used as an effective microtubule-disrupting agent, and many recent studies have used it for the activation of spindle checkpoint. In this study, we sought to identify the potential nocodazole target genes by profiling the gene expression pattern in HeLa cells. When treated with 0.1ug/ml of nocodazole, cells were efficiently arrested in the mitotic phase. HeLa cells also showed a higher proportion of apoptosis after drug treatment for a prolonged period. By DNA chip assay, we discovered that 50 genes changed their expressions in the nocodazole-treated cells with a minimal 2.0-fold change at 18h post-treatment. The majority of the differentially expressed genes belonged to two functional groups--genes involved in transcription regulation and in cellular signaling. These observations could have significant implications for our understanding of the physiological and pathophysiological regulation of spindle disrupting agents in human cells.
Cho
Sung Gook
SG
Department of Biology, Kyung Hee University, 130-701 Seoul, Republic of South Korea.
Sihn
Choong-Ryoul
CR
Yoo
Soon Ji
SJ
Cho
Kwang Keun
KK
Lee
Hong-Gu
HG
Choi
Yun-Jaie
YJ
Kim
Sang Hoon
SH
D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000187:drug effects*; D008938:Mitosis / Q000187:drug effects; D015739:Nocodazole / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D015739:Nocodazole