Triallelic patterns in STR loci used for paternity analysis: evidence for a duplication in chromosome 2 containing the TPOX STR locus.
We report triallelic patterns in several short tandem repeat (STR) loci revealed by routine paternity testing using the commercial AMPFlSTR Profiler and AMPFlSTR SGMplus kits. One case where the TPOX-locus (2p25.3) produced three peaks from the blood sample of a child was analysed further. Quantitative polymerase chain reaction (QPCR) and STR typing of the DNAs of the family trio revealed a large (>1.59 Mb) duplication flanking the TPOX-locus in chromosome 2 in both the mother and child. The implications of such genetic anomalies for paternity testing are discussed.
Lukka
Matti
M
Paternity Testing Laboratory, Department of Molecular Medicine, National Public Health Institute, Mannerheimintie 166, 00300 Helsinki, Finland.
Tasa
Gunnar
G
Ellonen
Pekka
P
Moilanen
Kirsi
K
Vassiljev
Vitali
V
Ulmanen
Ismo
I
D002648:Child; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D019075:Electrophoresis, Capillary; D005260:Female; D053803:Forensic Genetics; D020440:Gene Duplication; D005819:Genetic Markers; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
D005819:Genetic Markers