Single step purification, characterization and N-terminal sequences of a mannose specific lectin from mulberry seeds.
A mannose/glucose specific lectin have been isolated and purified from mulberry seeds by affinity chromatography on ConA Sepharose. The lectin is monomer in nature as judged by SDS-PAGE and its MW was estimated to be 22,000. The lectin is glycoprotein with neutral sugar content of 28.57%, and mannose and glucose were identified as carbohydrate. The lectin agglutinated rat red blood cells and in a hapten inhibition test, D: -mannose and D: -glucose was found to be inhibitor. The lectin also exhibited cytotoxic effect in brine shrimp lethality bioassay. The N-terminal sequences of the lectin upto 45-residues except the positions of 21, 39, 42 and 44 were identified. Sequence homology of the lectin is also discussed.
Absar
Nurul
N
Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, 6205, Bangladesh. nabsar5l@yahoo.com
Yeasmin
T
T
Raza
M Salim
MS
Sarkar
S Kumar
SK
Arisaka
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D006384:Hemagglutination; D037241:Mannose-Binding Lectins / Q000737:chemistry* / Q000302:isolation & purification* / Q000633:toxicity; D008970:Molecular Weight; D031625:Morus / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification; D011041:Poisoning; D051381:Rats; D012639:Seeds / Q000737:chemistry*
D002241:Carbohydrates; D037241:Mannose-Binding Lectins; D037121:Plant Lectins