title: [A method of HPRE synthesis via transcription by T7 RNA polymerase in vitro].
abstract: OBJECTIVE
  To synthesize highly pure HBV post-transcriptional regulatory element (HPRE) via transcription in vitro by T7 RNA polymerase.
  
  
  METHODS
  HPRE gene was amplified by PCR from a template containing HBV complete genomic DNA and cloned into plasmid pGEM-11zf. The cloned DNA sequence was transcribed by T7 RNA polymerase.
  
  
  RESULTS
  The construction of HPRE gene recombinant plasmid and production of HPRE via transcription in vitro was successful.
  
  
  CONCLUSIONS
  In vitro transcription by T7 RNA polymerase can be used to synthesize highly pure HPRE.
authors.affiliation: Key Laboratory of Infectious Diseases, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400016, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Jin-jun
authors.initials: JJ
authors.lastname: Guo
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Wei-xian
authors.initials: WX
authors.lastname: Chen
authors.forename: Ai-long
authors.initials: AL
authors.lastname: Huang
mesh_terms: D004259:DNA-Directed DNA Polymerase; D012321:DNA-Directed RNA Polymerases; D006515:Hepatitis B virus / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000502:physiology; D014158:Transcription, Genetic; D014764:Viral Proteins
chemical_list: D016601:RNA-Binding Proteins; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase