title: Toscana virus in Spain.
abstract: Toscana virus (TOSV, Phlebovirus, family Bunyaviridae) infection is one of the most prevalent arboviruses in Spain. Within the objectives of a multidisciplinary network, a study on the epidemiology of TOSV was conducted in Granada, in southern Spain. The overall seroprevalence rate was 24.9%, significantly increasing with age. TOSV was detected in 3 of 103 sandfly pools by viral culture or reverse transcription-polymerase chain reaction from a region of the L gene. Nucleotide sequence homology was 99%-100% in TOSV from vectors and patients and 80%-81% compared to the Italian strain ISS Phl.3. Sequencing of the N gene of TOSV isolates from patients and vectors indicated 87%-88% and 100% homology at the nucleotide and amino acid levels, respectively, compared to the Italian strain. These findings demonstrate the circulation of at least 2 different lineages of TOSV in the Mediterranean basin, the Italian lineage and the Spanish lineage.
authors.affiliation: Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avda. Fuerzas Armadas, s/n 18014-Granada, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Sanbonmatsu-Gámez
authors.forename: Mercedes
authors.initials: M
authors.lastname: Pérez-Ruiz
authors.forename: Ximena
authors.initials: X
authors.lastname: Collao
authors.forename: María Paz
authors.initials: MP
authors.lastname: Sánchez-Seco
authors.forename: Francisco
authors.initials: F
authors.lastname: Morillas-Márquez
authors.forename: Manuel
authors.initials: M
authors.lastname: de la Rosa-Fraile
authors.forename: José Maria
authors.initials: JM
authors.lastname: Navarro-Mari
authors.forename: Antonio
authors.initials: A
authors.lastname: Tenorio
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007303:Insect Vectors / Q000821:virology*; D008297:Male; D010691:Phlebotomus / Q000821:virology*; D010217:Phlebotomus Fever / Q000453:epidemiology* / Q000821:virology; D010802:Phylogeny; D015995:Prevalence; D029301:Sandfly fever Naples virus / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013030:Spain / Q000453:epidemiology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014764:Viral Proteins