title: Rapid separation of strychnine and brucine on a dynamically modified poly(dimethylsiloxane) microchip followed by electrochemical detection.
abstract: A method has been developed for rapidly separating and detecting strychnine and brucine using a poly(dimethysiloxane) (PDMS) microchip and electrochemical (EC) detection. PDMS microchannels dynamically modified by Brij35 are shown to be more efficient than native ones. The two analytes are well separated within 90 s in 70 mmol/L acetate buffer (pH 5.5) containing 0.01% (v/v) Brij35. Detection limits were found to be 1.0 micromol/L for strychnine and 0.2 micromol/L for brucine at S/N = 3. The method was used to determine trace strychnine and brucine in rat serum, and the results obtained correlate well with those obtained via high-performance liquid chromatography (HPLC).
authors.affiliation: Key Lab of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, 210093, Nanjing, PR China.
authors.forename: Q L
authors.initials: QL
authors.lastname: Zhang
authors.forename: J J
authors.initials: JJ
authors.lastname: Xu
authors.forename: H Z
authors.initials: HZ
authors.lastname: Lian
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
authors.forename: H Y
authors.initials: HY
authors.lastname: Chen
mesh_terms: D000818:Animals; D002021:Buffers; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D046228:Microarray Analysis / Q000379:methods*; D015394:Molecular Structure; D011092:Polyethylene Glycols; D051381:Rats; D015203:Reproducibility of Results; D013331:Strychnine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D002021:Buffers; D004129:Dimethylpolysiloxanes; D011092:Polyethylene Glycols; C083806:brucine; D013331:Strychnine; C515901:Brij 35