[Effects of glycyrrhizin on TGFbeta1 stimulated hepatic stellate cell signaling transduction].
OBJECTIVE
To investigate the role of glycyrrhizin on TGFbeta1 stimulated signaling transduction in rat hepatic stellate cells (HSCs).


METHODS
The mice HSCs were isolated and cultured with or without glycyrrhizin (1 micromol/L-1000 micromol/L) in vitro after TGFbeta1 stimulation. The mRNA level of Smad2, 3, 7 were measured with RT-PCR; protein expression level of Smad2, 3, 7 and collagen I, III were analyzed with Western blot.


RESULTS
TGFbeta1 increased the mRNA level and protein expression of Smad2, 3, 7 in HSC; it also increased protein expression of collagen I and III. 1 micromol/L-1000 micromol/L glycyrrhizin decreased the mRNA level and protein expression of Smad2, 3, 7; it also inhibited protein expression of collagen I and III gradually.


CONCLUSIONS
Interventing the TGFbeta signaling pathway and decreasing the synthesis of collagen, might be involved in the anti-fibrosis mechanism of glycyrrhizin.
Dong
Ling
L
Department of Digestive Diseases, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Sun
Jian-yong
JY
Fang
Guo-ting
GT
Jiang
Lin-di
LD
Wang
Ji-yao
JY
D000818:Animals; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D022781:Hepatocytes / Q000378:metabolism*; D008297:Male; D051381:Rats; D015398:Signal Transduction; D051785:Smad Proteins / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D051785:Smad Proteins; D016212:Transforming Growth Factor beta; D019695:Glycyrrhizic Acid