title: Global synthetic-lethality analysis and yeast functional profiling.
abstract: The Saccharomyces genome-deletion project created >5900 'molecularly barcoded' yeast knockout mutants (YKO mutants). The YKO mutant collections have facilitated large-scale analyses of a multitude of mutant phenotypes. For example, both synthetic genetic array (SGA) and synthetic-lethality analysis by microarray (SLAM) methods have been used for synthetic-lethality screens. Global analysis of synthetic lethality promises to identify cellular pathways that 'buffer' each other biologically. The combination of global synthetic-lethality analysis, together with global protein-protein interaction analyses, mRNA expression profiling and functional profiling will, in principle, enable construction of a cellular 'wiring diagram' that will help frame a deeper understanding of human biology and disease.
authors.affiliation: High Throughput Biology Center, Institute of Genetic Medicine, Department of Biostatistics, Johns Hopkins University School of Medicine, 339 Broadway Research Building, 733 North Broadway, Baltimore, MD 21205, USA.
authors.forename: Siew Loon
authors.initials: SL
authors.lastname: Ooi
authors.forename: Xuewen
authors.initials: X
authors.lastname: Pan
authors.forename: Brian D
authors.initials: BD
authors.lastname: Peyser
authors.forename: Ping
authors.initials: P
authors.lastname: Ye
authors.forename: Pamela B
authors.initials: PB
authors.lastname: Meluh
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Yuan
authors.forename: Rafael A
authors.initials: RA
authors.lastname: Irizarry
authors.forename: Joel S
authors.initials: JS
authors.lastname: Bader
authors.forename: Forrest A
authors.initials: FA
authors.lastname: Spencer
authors.forename: Jef D
authors.initials: JD
authors.lastname: Boeke
mesh_terms: D002874:Chromosome Mapping; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D005804:Genes, Lethal; D005821:Genetic Techniques; D016681:Genome, Fungal; D020411:Oligonucleotide Array Sequence Analysis; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger