Production of L-malic acid by permeabilized cells of commercial Saccharomyces sp. strains.
Of various yeasts tested in the conversion of fumaric to L-malic acid, Saccharomyces bayanus had the highest activity of fumarase. Cells permeabilized with 0.2% (w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times higher (271 U/g) than that of the intact cells (67 U/g). The proposed mathematical model for the batch production of L-malic acid was validated at different initial fumaric acid concentrations. The average conversion of fumaric acid was up to 82% and gave 21, 40, 83 and 175 mM L-malic acid from respectively, 25, 50, 100 and 210 mM: fumaric acid.
Presecki
Ana Vrsalović
AV
Faculty of Chemical Engineering and Technology, University of Zagreb, Savska c. 16, HR-10000 Zagreb, Croatia.
Vasić-Racki
Durda
D
D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D005649:Fumarate Hydratase / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism; D007700:Kinetics; D008293:Malates / Q000378:metabolism*; D012440:Saccharomyces / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D013045:Species Specificity; D013501:Surface-Active Agents / Q000494:pharmacology; D013997:Time Factors
D002593:Cetrimonium Compounds; D005650:Fumarates; D008293:Malates; D013501:Surface-Active Agents; C030298:malic acid; C032005:fumaric acid; D005649:Fumarate Hydratase; D000077286:Cetrimonium