Chromosomal fragmentation is the major consequence of the rdgB defect in Escherichia coli.
The rdgB mutants depend on recombinational repair of double-strand breaks. To assess other consequences of rdgB inactivation in Escherichia coli, we isolated RdgB-dependent mutants. All transposon inserts making cells dependent on RdgB inactivate genes of double-strand break repair, indicating that chromosomal fragmentation is the major consequence of RdgB inactivation.
Lukas
Lisa
L
Department of Microbiology, University of Illinois, Urbana, Illinois 61801, USA.
Kuzminov
Andrei
A
D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D020868:Gene Silencing; D009154:Mutation; D011687:Purines / Q000633:toxicity; D011743:Pyrimidines / Q000633:toxicity; D011755:Pyrophosphatases / Q000235:genetics*
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D011687:Purines; D011743:Pyrimidines; D011755:Pyrophosphatases; C474645:RdgB protein, E coli