title: Y-chromosomal STR haplotypes in a Northeast Italian population sample using 17plex loci PCR assay.
abstract: One hundred fifty-five unrelated, autochthonous healthy males from Northeast Italy were typed for the 17 Y-chromosome short tandem repeat (STR) (Y-STR) loci DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATA H4, DYS437, DYS438, DYS448 using the AmpFLSTR Yfiler polymerase chain reaction amplification kit. A total of 153 different haplotypes were observed, and among these, 151 were unique, while 2 were found two times. The overall haplotype diversity was 0.9997. Furthermore, 50 father-son pairs, previously confirmed by autosomal STR analysis, were typed using the same set of 17 Y-STR loci, and, among 850 allele transfers, three mutation events were identified, giving an average mutation rate of 3.53 x 10(-3) per locus per generation (95% confidence interval 0.73-1.03).
authors.affiliation: Department of Medicine and Public Health, Institute of Legal Medicine, Forensic Genetic Laboratory, University of Verona, Policlinico G.B. Rossi, Piazzale L.A. Scuro, 37134, Verona, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Turrina
authors.forename: Renzo
authors.initials: R
authors.lastname: Atzei
authors.forename: Domenico
authors.initials: D
authors.lastname: De Leo
mesh_terms: D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D007558:Italy; D008297:Male; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences