Enhancement of 1,3-propanediol production by Klebsiella pneumoniae with fumarate addition.
Addition of 5 mM: fumarate to cultures of Klebsiella pneumoniae enhanced the rate of glycerol consumption and the production of 1,3-propanediol (PDO). Compared to the control, the activity of glycerol dehydrogenase increased by 35, 33 and 46%, the activity of glycerol dehydratase increased by 160, 210 and 115%, and the activity of 1,3-propanediol oxidoreductase increased by 25, 39 and 85% when, respectively, 5, 15 and 25 mM: fumarate were provided. At the same time, the ratio of NAD+ to NADH decreased by 20, 23 and 29%. Using a 5 l bioreactor with 5 mM: fumarate addition, the specific rate of glycerol consumption and the productivity of PDO was 30 mmol/l h and 17 mmol/l h, respectively, both increased by 35% over the control.
Lin
Rihui
R
Department of Chemical Engineering, Tsinghua University, 100084, Beijing, P.R. China, dhliu@tsinghua.edu.cn.
Liu
Hongjuan
H
Hao
Jian
J
Cheng
Keke
K
Liu
Dehua
D
D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005650:Fumarates / Q000008:administration & dosage*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology; D005990:Glycerol / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000502:physiology*; D011409:Propylene Glycols / Q000302:isolation & purification* / Q000378:metabolism*
D005650:Fumarates; D011409:Propylene Glycols; C041787:1,3-propanediol; C032005:fumaric acid; D006836:Hydro-Lyases; C020671:glycerol dehydratase; D005990:Glycerol