title: Subventricular zone neuroblasts emigrate toward cortical lesions.
abstract: Adult subventricular zone (SVZ) neuroblasts migrate in the rostral migratory stream to the olfactory bulbs. Brain lesions generally increase SVZ neurogenesis or gliogenesis and cause SVZ cell emigration to ectopic locations. We showed previously that glia emigrate from the SVZ toward mechanical injuries of the somatosensory cerebral cortex in mice. Here we tested the hypotheses that SVZ neurogenesis increases, that neuroblasts emigrate, and that epidermal growth factor expression increases after cortical injuries. Using immunohistochemistry for phenotypic markers and BrdU, we show that newborn doublecortin-positive SVZ neuroblasts emigrated toward cerebral cortex lesions. However, the number of doublecortin-positive cells in the olfactory bulbs remained constant, suggesting that dorsal emigration was not at the expense of rostral migration. Although newborn neuroblasts emigrated, rates of SVZ neurogenesis did not increase after cortical lesions. Finally, we examined molecules that may regulate emigration and neurogenesis after cortical lesions and found that epidermal growth factor was increased in the SVZ, corpus callosum, and cerebral cortex. These results suggest that after injuries to the cerebral cortex, neuroblasts emigrate from the SVZ, that emigration does not depend either on redirection of SVZ cells or on increased neurogenesis, and that epidermal growth factor may induce SVZ emigration.
authors.affiliation: CMRC Neurobiology Program, Children's Memorial Hospital, Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60614-3394, USA.
authors.forename: Nikki L
authors.initials: NL
authors.lastname: Sundholm-Peters
authors.forename: Helen K C
authors.initials: HK
authors.lastname: Yang
authors.forename: Gwendolyn E
authors.initials: GE
authors.lastname: Goings
authors.forename: Avery S
authors.initials: AS
authors.lastname: Walker
authors.forename: Francis G
authors.initials: FG
authors.lastname: Szele
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development / Q000473:pathology / Q000503:physiopathology*; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology*; D002465:Cell Movement; D002540:Cerebral Cortex / Q000473:pathology / Q000503:physiopathology*; D002552:Cerebral Ventricles; D004815:Epidermal Growth Factor / Q000378:metabolism; D008297:Male; D051379:Mice; D009474:Neurons / Q000473:pathology; D009830:Olfactory Bulb / Q000473:pathology / Q000503:physiopathology; D013234:Stem Cells / Q000473:pathology
chemical_list: D004815:Epidermal Growth Factor