A new approach to evaluate stability of amodiaquine and its metabolite in blood and plasma.
A stability study for amodiaquine (AQ) and desethylamodiaquine (AQm) in whole blood and plasma is reported. AQ, AQm and chloroquine (CQ) were simultaneously analysed and the ratios AQ/CQ and AQm/CQ were used to ensure correct interpretation of the stability results. CQ was stable in whole blood and plasma at all tested temperatures enabling it to be a stability marker in stability studies. Simultaneous analysis of compounds, of which at least one is already known to be stable, permits a within sample ratio to be used as a stability indicator. The new approach significantly reduced bias when compared to the traditional approach. AQ and AQm were stable in plasma at -86 degrees C and -20 degrees C for 35 days, at 4 degrees C for 14 days and at 22 degrees C for 1 day. AQ and AQm were stable in blood at -86 degrees C and 4 degrees C for 35 days, at -20 degrees C and 22 degrees C for 7 days and at 37 degrees C for 1 day.
Blessborn
D
D
Dalarna University College, S-781 88 Borlänge, Sweden.
Neamin
G
G
Bergqvist
Y
Y
Lindegårdh
N
N
D000655:Amodiaquine / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D000818:Animals; D000962:Antimalarials / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics; D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D008956:Models, Chemical; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D000962:Antimalarials; D000655:Amodiaquine