Use of denaturing high-performance liquid chromatography for rapid detection and identification of seven Candida species.
A novel denaturing high-performance liquid chromatography (DHPLC)-based technique allows rapid high-resolution analysis of PCR products. We used this technique for unequivocal molecular identification of seven Candida species. We show the application of this PCR/DHPLC approach for direct detection and identification of yeast species from blood cultures and for detection of Candida colonization in the gastrointestinal tract of allogeneic transplant patients.
Goldenberg
Oliver
O
Institute of Microbiology and Hygiene, Charité Universitaetsmedizin Berlin, Campus Charité Mitte, Dorotheenstrasse 96, D-10117 Berlin, Germany.
Herrmann
Stefanie
S
Adam
Thomas
T
Marjoram
Gina
G
Hong
George
G
Göbel
Ulf B
UB
Graf
Barbara
B
D001769:Blood / Q000382:microbiology; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D005243:Feces / Q000382:microbiology; D016469:Fungemia / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D013997:Time Factors
D003470:Culture Media; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer