Immunolocalization of cytoskeletal components in the tegument of the 3-week-old juvenile and adult Fasciola gigantica.
Components of three cytoskeletal elements, namely, microtubule, intermediate and actin filaments have been localised in the tegument of the 3-week-old juvenile and adult Fasciola gigantica by means of immunofluorescence and immunoperoxidase techniques, using mouse monoclonal anti-alpha-tubulin, anti-cytokeratin antibodies and biotinylated-phalloidin, respectively. The immunostaining with the above probes were also performed in adult Schistosoma mansoni for comparison. The presence of tubulin, indicative of microtubules, was demonstrated in the tegumental cell bodies, their cytoplasmic processes, and the basal layer of the tegumental syncytium of F. gigantica. While in S. mansoni, tubulin appeared as vertical lines stretching across the whole thickness of the syncytium. Cytokeratin, representing one type of intermediate filaments, was detected in the tegumental cell bodies, their cytoplasmic processes, tegumental syncytium and spines of F. gigantica. In contrast, cytokeratin was evident only in the syncytium of S. mansoni, but not in the spines. Phalloidin, which could bind to actin, a subunit of microfilament, was detected in the tegumental cell bodies, their processes, and the microtrabecular network which form the scaffold of the tegumental syncytium of F. gigantica. In S. mansoni, actin was localized in similar tissues except the syncytium was not stained while spines exhibited intense staining. In F. gigantica, the presence of microtubules and actin filaments in the tegumental cells, their processes and in the syncytium could mediate the movement of secretory granules from the cell bodies towards the basal as well as the apical layer of the tegument. Cytokeratin filaments may serve to reinforce the integrity of the tegumental syncytium as well as the spines.
Tansatit
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Department of Pathobiology, Faculty of Science, Mahidol University, RamaVI Rd., Bangkok 10400, Thailand.
Sahaphong
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S
Riengrojpitak
S
S
Viyanant
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V
Sobhon
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P
D000199:Actins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D019921:Biotinylation / Q000379:methods / Q000662:veterinary; D003599:Cytoskeleton / Q000276:immunology*; D005209:Fasciola / Q000737:chemistry* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique / Q000379:methods / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000379:methods / Q000662:veterinary; D007633:Keratins / Q000032:analysis* / Q000276:immunology; D012550:Schistosoma mansoni / Q000737:chemistry / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D014404:Tubulin / Q000032:analysis* / Q000276:immunology
D000199:Actins; D000911:Antibodies, Monoclonal; D014404:Tubulin; D007633:Keratins