"Wide-open" 1.3 A structure of a multidrug-resistant HIV-1 protease as a drug target.
This report examines structural changes in a highly mutated, clinical multidrug-resistant HIV-1 protease, and the crystal structure has been solved to 1.3 A resolution in the absence of any inhibitor. This protease variant contains codon mutations at positions 10, 36, 46, 54, 62, 63, 71, 82, 84, and 90 that confer resistance to protease inhibitors. Major differences between the wild-type and the variant include a structural change initiated by the M36V mutation and amplified by additional mutations in the flaps of the protease, resulting in a "wide-open" structure that represents an opening that is 8 A wider than the "open" structure of the wild-type protease. A second structural change is triggered by the L90M mutation that results in reshaping the 23-32 segment. A third key structural change of the protease is due to the mutations from longer to shorter amino acid side chains at positions 82 and 84.
Martin
Philip
P
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Vickrey
John F
JF
Proteasa
Gheorghe
G
Jimenez
Yurytzy L
YL
Wawrzak
Zdzislaw
Z
Winters
Mark A
MA
Merigan
Thomas C
TC
Kovari
Ladislau C
LC
D000595:Amino Acid Sequence; D001665:Binding Sites; D003062:Codon / Q000235:genetics; D003461:Crystallography; D024921:Drug Resistance, Multiple, Viral; D016333:HIV Protease / Q000737:chemistry* / Q000235:genetics*; D017320:HIV Protease Inhibitors / Q000494:pharmacology; D015497:HIV-1 / Q000201:enzymology*; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation; D014867:Water / Q000737:chemistry
D003062:Codon; D017320:HIV Protease Inhibitors; D014867:Water; D016333:HIV Protease