Transient NMDA receptor-mediated hypoperfusion following umbilical cord occlusion in preterm fetal sheep.
Exposure to severe hypoxia leads to delayed cerebral and peripheral hypoperfusion. There is evidence in the very immature brain that transient abnormal glutaminergic receptor activity can occur during this phase of recovery. We therefore examined the role of N-methyl-D-aspartate (NMDA) receptor activity in mediating secondary hypoperfusion in preterm fetal sheep at 70% of gestation. Fetuses received either sham asphyxia or asphyxia and were studied for 12 h recovery. The specific, non-competitive NMDA receptor antagonist dizocilpine maleate (2 mg kg-1 bolus plus 0.07 mg kg h-1i.v.) or saline (vehicle) was infused from 15 min after asphyxia until 4 h. In the asphyxia-vehicle group abnormal epileptiform EEG transients were observed during the first 4 h of reperfusion, the peak of which corresponded approximately to the nadir in peripheral and cerebral hypoperfusion. Dizocilpine significantly suppressed this activity (2.7+/-1.3 versus 11.2+/-2.7 counts min-1 at peak frequency, P<0.05) and markedly delayed and attenuated the rise in vascular resistance in both peripheral and cerebral vascular beds observed after asphyxia, effectively preventing the initial deep period of hypoperfusion in carotid blood flow and femoral blood flow (P<0.01). However, while continued infusion did attenuate subsequent transient tachycardia, it did not prevent the development of a secondary phase of persistent but less profound hypoperfusion. In conclusion, the present studies suggest that in the immature brain the initial phase of delayed cerebral and peripheral hypoperfusion following exposure to severe hypoxia is mediated by NMDA receptor activity. The timing of this effect in the cerebral circulation corresponds closely to abnormal EEG activity, suggesting a pathological glutaminergic activation that we speculate is related to evolving brain injury.
Dean
Justin M
JM
Department of Physiology, Faculty of Medicine and Health Science, The University of Auckland, Private Bag 92019, Auckland, New Zealand.
Gunn
Alistair Jan
AJ
Wassink
Guido
G
Bennet
Laura
L
D000818:Animals; D001237:Asphyxia / Q000196:embryology / Q000378:metabolism* / Q000503:physiopathology; D001921:Brain / Q000196:embryology / Q000378:metabolism* / Q000503:physiopathology; D002339:Carotid Arteries / Q000187:drug effects / Q000196:embryology / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000187:drug effects; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005263:Femoral Artery / Q000187:drug effects / Q000196:embryology / Q000503:physiopathology; D005311:Fetal Hypoxia / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D005333:Fetus / Q000098:blood supply / Q000378:metabolism* / Q000503:physiopathology; D005865:Gestational Age; D006340:Heart Rate, Fetal; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012756:Sheep; D013997:Time Factors; D014470:Umbilical Cord / Q000601:surgery; D014655:Vascular Resistance
D018691:Excitatory Amino Acid Antagonists; D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate