Simultaneous determination of vitamins A, E and 25-OH-vitamin D: application in clinical assessments.
OBJECTIVE
To optimize an HPLC method for the simultaneous determination of vitamins A, E and 25-OH-vitamin D.


METHODS
Modifications include sample volume, internal standard, temperature, detection and injection volume.


CONCLUSIONS
Validity was supported by performance in interlaboratory exercises. Utility and versatility in clinical practice were assessed in outpatients and hospitalized subjects. The method was shown to be accurate and reliable for the simultaneous determination of vitamins A, E and 25-OH-vitamin D in serum throughout the entire range of clinically relevant concentrations.
Granado-Lorencio
F
F
Unidad de Vitaminas, Servicio de Endocrinología y Nutrición, Hospital Universitario Puerta de Hierro, 28035 Madrid, Spain.
Olmedilla-Alonso
B
B
Herrero-Barbudo
C
C
Blanco-Navarro
I
I
Blázquez-García
S
S
Pérez-Sacristán
B
B
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009752:Nutritional Status; D015203:Reproducibility of Results; D014801:Vitamin A / Q000097:blood*; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood; D014810:Vitamin E / Q000097:blood*
D014801:Vitamin A; D014807:Vitamin D; D014810:Vitamin E; C104450:25-hydroxyvitamin D