The recombination deficient Enterococcus faecalis UV202 strain is a recA mutant.
The recA gene of the recombination deficient Enterococcus faecalis strain UV202 was sequenced and found to encode a glycine to aspartic acid mutation at amino acid 265. Both the UV sensitive and recombination deficient phenotypes of the UV202 strain were complemented by expression of the wild-type recA gene cloned under the control of the nisin-inducible promoter of an expression vector.
Weaver
Keith E
KE
Division of Basic Biomedical Sciences, School of Medicine, University of South Dakota, Vermillion, SD 57069, USA. kweaver@usd.edu
Reddy
Shirisha G
SG
D003001:Cloning, Molecular; D013293:Enterococcus faecalis / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects; D004790:Enzyme Induction / Q000235:genetics / Q000528:radiation effects; D005816:Genetic Complementation Test; D009154:Mutation; D011938:Rec A Recombinases / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
D011938:Rec A Recombinases