Deficiency of uridine monophosphate synthase (DUMPS) and X-chromosome deletion in fetal mummification in cattle.
Ten mummified fetuses were tested for the deficiency of uridine monophosphate synthase (DUMPS), which is known to contribute to the embryonic and fetal mortality in cattle. Genomic DNAs of the mummified fetuses were extracted from tissue samples collected from the mummies and were amplified by GenomiPhi DNA amplification kit. UMPS gene of the mummies was amplified by polymerase chain reaction (PCR) with DUMPS primers. Out of ten mummies examined, two fetuses were heterozygous (carriers) for DUMPS as indicated by the presences of three bands of 89, 53 and 36 bp. Estimated stage of gestation when the death occurred in the two mummies was 3.5 and 2.5 months, respectively. The other fetuses exhibited only two bands of 53 and 36 bp on the polyacrylamide gel indicated that they were normal. On the other hand, all the mummies were sexed using AMX/Y primers. Specific regions of Y and X chromosomes were amplified by PCR using AMX/Y. The expected 280 bp fragment in the female sample and the 280 and 217 bp in the male sample were observed. Nine mummies had a normal X and Y chromosome bands; however, the other mummified fetus exhibited only Y chromosome band, while the constitutive X chromosome fragment was missing. The estimated stage of gestation when the death occurred in this mummified fetus was 100 days. This might be the first report of DUMPS and X-chromosome deletion at the amelogenin gene in bovine-mummified fetuses in Japan.
Ghanem
Mohamed Elshabrawy
ME
Laboratory of Animal Science, Graduate School for International Development and Cooperation, Hiroshima University, 1-5-1 Kagamiyama, Higashi-Hiroshima 739-8529, Japan.
Nakao
Toshihiko
T
Nishibori
Masahide
M
D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D002418:Cattle Diseases / Q000201:enzymology* / Q000235:genetics*; D002872:Chromosome Deletion; D004247:DNA / Q000032:analysis; D005260:Female; D005333:Fetus / Q000201:enzymology*; D005784:Gene Amplification; D009097:Multienzyme Complexes / Q000172:deficiency* / Q000235:genetics; D009962:Orotate Phosphoribosyltransferase / Q000172:deficiency* / Q000235:genetics; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000172:deficiency* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011247:Pregnancy; D019849:Sex Determination Processes; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics
D009097:Multienzyme Complexes; C027157:uridine 5'-monophosphate synthase; D004247:DNA; D009962:Orotate Phosphoribosyltransferase; D009964:Orotidine-5'-Phosphate Decarboxylase