Design and synthesis of a Tat-related gene transporter: a tool for carrying the adenovirus vector into cells.
A Tat-related peptide, acetyl-Gly-Arg-Arg-Arg-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Pro-Gln-Gly-Cys amide, designed to transport an Adenovirus vector (Ad) into cells, was synthesized. The synthetic peptide was conjugated to Ad, which potentially can act as an efficient carrier of heterologous genes into cells. The Tat-related peptide was synthesized using the solid phase method and then was coupled to the heterofunctional cross-linking reagent, 6-maleimidohexanoic acid N-hydroxysuccinimide ester. The resulting peptide-succinimidohexanoic acid N-hydroxysuccinimide ester was conjugated to Ad containing the luciferase gene. B16BL6 cells infected with the peptide-conjugated Ad luciferase gene construct exhibit a 50-fold greater luciferase activity than B16BL6 cells infected with wild-type Ad containing the luciferase gene.
Kida
Shinya
S
Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Nishi-ku, Kobe 651-2180, Japan.
Maeda
Mitsuko
M
Hojo
Keiko
K
Eto
Yusuke
Y
Gao
Jian-Qing
JQ
Kurachi
Shinnosuke
S
Mizuguchi
Hiroyuki
H
Hayakawa
Takao
T
Mayumi
Tadanori
T
Nakagawa
Shinsaku
S
Kawasaki
Koichi
K
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry; D015195:Drug Design; D015696:Gene Products, tat / Q000138:chemical synthesis / Q000187:drug effects / Q000378:metabolism* / Q000494:pharmacology; D005822:Genetic Vectors; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000494:pharmacology; D013388:Succinimides / Q000737:chemistry; D014161:Transduction, Genetic
D003432:Cross-Linking Reagents; D015696:Gene Products, tat; D009842:Oligopeptides; D013388:Succinimides; C508949:acetyl-Gly-Arg-Arg-Arg-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Pro-Gln-Gly-Cys amide; D008156:Luciferases