Response of osteoblastic cells to titanium submitted to three different surface treatments.
In the complex process of bone formation at the implant-tissue interface, surface properties are relevant factors modulating osteoblastic function. In this study, commercially pure titanium (cp Ti) samples were prepared with different surface characteristics using chemical attack with a sulfuric acid/hydrochloric acid based solution (treatment A); chemical attack plus anodic oxidation using phosphoric acid (treatment B); and chemical attack plus thermal oxidation followed by immersion in a sodium fluoride solution (treatment C). The samples were characterized by scanning electron microscopy (SEM), contact profilometry and contact angle. The biological performance of the prepared surfaces was evaluated using mice osteoblastic cell cultures for up to 21 days. Cells seeded on the different titanium samples showed similar behavior during cell attachment and spreading. However, cellular proliferation and differentiation were higher for samples submitted to treatments A and C (p < or = 0.05; n = 3), which were less rough and showed surface free energy with smaller polar components.
Santiago
Adriana Soares
AS
Metallurgical and Materials Department, Alberto Luiz Coimbra Graduate Studies and Engineering Research Institute, Federal University of Rio de Janeiro.
Santos
Euler Araújo dos
EA
Sader
Márcia Soares
MS
Santiago
Marcelo Felippe
MF
Soares
Gloria de Almeida
Gde A
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D003764:Dental Materials / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000494:pharmacology; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D010084:Oxidation-Reduction; D010756:Phosphoric Acids / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology; D013464:Sulfuric Acids / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology
D001672:Biocompatible Materials; D003764:Dental Materials; D010756:Phosphoric Acids; D013464:Sulfuric Acids; D012969:Sodium Fluoride; D014025:Titanium; C030242:phosphoric acid; C033158:sulfuric acid; D006851:Hydrochloric Acid