[Photomacrography of brain surface for evaluating blood-brain barrier disruption within 24 h after focal cerebral ischemia in mice].
To establish a new photomacrographic analysis of morphological changes on brain surface to evaluate blood-brain barrier (BBB) disruption.



Permanent focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in mice. Brains were removed 10 min, 0.5, 1, 3, 6, 12 and 24 h after MCAO. The whole brains and brain slices were photographed by a digital camera. BBB disruption was evaluated by hemorrhage and traced Evans blue (EB) on the brain surface. Fluoremetric quantitation of EB and water content in the brains were also performed at various time points.



Photomacrographic morphological analysis showed that hemorrhage and traced EB on the surface of the brains significantly increased from 3 h after focal cerebral ischemia,which were correlated to the results in the brain slices. EB content in the ischemic hemispheres was significantly increased from 0.5 h after MCAO, and water content was increased from 1 h after MCAO.



Photomacrographic measurement is a simple and useful method for evaluating BBB disruption semi-quantitatively, and can detect BBB disruption earlier after focal cerebral ischemia in mice.
Chen
Li-ping
LP
Department of Pharmacology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Xu
Hui-min
HM
Zhao
Wei
W
Zhang
Shi-hong
SH
Zhu
Zhao-yang
ZY
Zhang
Qi
Q
Yu
Guo-liang
GL
Chu
Sheng-li
SL
Wei
Er-qing
EQ
D000818:Animals; D001812:Blood-Brain Barrier / Q000503:physiopathology*; D001921:Brain / Q000503:physiopathology*; D005070:Evans Blue; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010781:Photography; D013997:Time Factors
D005070:Evans Blue