title: Analysis of gene expression induced by microtubule-disrupting agents in HeLa cells using microarray.
abstract: Microtubules are important cytoskeletal elements that have been shown to play a major role in many cellular processes because of their mechanical properties and/or their participation in various cell signaling pathways. Nocodazole is used as an effective microtubule-disrupting agent, and many recent studies have used it for the activation of spindle checkpoint. In this study, we sought to identify the potential nocodazole target genes by profiling the gene expression pattern in HeLa cells. When treated with 0.1ug/ml of nocodazole, cells were efficiently arrested in the mitotic phase. HeLa cells also showed a higher proportion of apoptosis after drug treatment for a prolonged period. By DNA chip assay, we discovered that 50 genes changed their expressions in the nocodazole-treated cells with a minimal 2.0-fold change at 18h post-treatment. The majority of the differentially expressed genes belonged to two functional groups--genes involved in transcription regulation and in cellular signaling. These observations could have significant implications for our understanding of the physiological and pathophysiological regulation of spindle disrupting agents in human cells.
authors.affiliation: Department of Biology, Kyung Hee University, 130-701 Seoul, Republic of South Korea.
authors.forename: Sung Gook
authors.initials: SG
authors.lastname: Cho
authors.forename: Choong-Ryoul
authors.initials: CR
authors.lastname: Sihn
authors.forename: Soon Ji
authors.initials: SJ
authors.lastname: Yoo
authors.forename: Kwang Keun
authors.initials: KK
authors.lastname: Cho
authors.forename: Hong-Gu
authors.initials: HG
authors.lastname: Lee
authors.forename: Yun-Jaie
authors.initials: YJ
authors.lastname: Choi
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Kim
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000187:drug effects*; D008938:Mitosis / Q000187:drug effects; D015739:Nocodazole / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D015739:Nocodazole