CC chemokines and chemokine receptors in IgA nephropathy (IgAN) and in non-IgA mesangial proliferative glomerulonephritis (MesProGN). the immunohistochemical comparative study.
The aim of the study was to compare the immunoexpression of monocyte chemoattractant protein-1 (MCP-1), monocyte inflammatory protein-1alpha (MIP-1alpha), Regulated upon Activation Normal T-cell Expressed and Secreted (RANTES) and CC chemokine receptors: CCR1 and CCR5 in renal biopsy specimens in IgA nephropathy (IgAN) and in non-IgA mesangial proliferative glomerulonephritis (MesProGN), and to find any relationships between the immunoexpression of chemokines and chemokine receptors and renal interstitial lesions. Our study revealed increased immunoexpression of tubulointerstitial MCP-1 (P < 0.02), RANTES (P < 0.03) and interstitial CCR5+ cells (P < 0.05) in IgAN as compared with MesProGN. In the renal tissue in patients with IgAN the intensity of tubulointerstitial immunostaining of MCP-1 and the number of CCRI+ cells and CCR5+ cells were significantly correlated with the renal interstitial cortical volume, meanwhile in biopsy specimens from patients with MesProGN only interstitial CCR5+ cells were positively correlated with the interstitial cortical volume. In conclusion, these observations suggest that in the renal tissue in IgAN patients MCP-1, RANTES and CCR5 are up-regulated as compared with renal biopsy specimens from patients with MesProGN. Moreover, MCP-1 as well CCR5 and CCR1 positive cells may play a role in the interstitial processes leading to fibrosis in IgAN, whereas in MesProGN CCR5-positive cells may participate in interstitial lesions in renal tissue.
Wagrowska-Danilewicz
Małgorzata
M
Department of Nephropathology, Chair of Pathomorphology, Medical University, Lódź. emwude@poczta.onet.pl
Danilewicz
Marian
M
Stasikowska
Olga
O
D000328:Adult; D018932:Chemokine CCL2 / Q000096:biosynthesis; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000096:biosynthesis; D019742:Chemokines, CC / Q000096:biosynthesis*; D005260:Female; D005922:Glomerulonephritis, IGA / Q000378:metabolism*; D015432:Glomerulonephritis, Membranoproliferative / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D019707:Receptors, Chemokine / Q000096:biosynthesis*; D015854:Up-Regulation
D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D019707:Receptors, Chemokine