title: Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus.
abstract: The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes have sufficient substrate promiscuity to permit the metabolism of both sugars. Recently, it has been proposed that the part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus as an alternative route for glucose metabolism. In this report we demonstrate, by in vitro kinetic studies of D-2-keto-3-deoxygluconate (KDG) kinase and KDG aldolase, that the part-phosphorylative pathway in S. solfataricus is also promiscuous for the metabolism of both glucose and galactose.
authors.affiliation: Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
authors.forename: Henry J
authors.initials: HJ
authors.lastname: Lamble
authors.forename: Alex
authors.initials: A
authors.lastname: Theodossis
authors.forename: Christine C
authors.initials: CC
authors.lastname: Milburn
authors.forename: Garry L
authors.initials: GL
authors.lastname: Taylor
authors.forename: Steven D
authors.initials: SD
authors.lastname: Bull
authors.forename: David W
authors.initials: DW
authors.lastname: Hough
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Danson
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000446:Aldehyde-Lyases / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001105:Archaea / Q000378:metabolism*; D001711:Biotransformation; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005690:Galactose / Q000378:metabolism; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D048229:Sulfolobus solfataricus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: D010455:Peptides; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C021471:2-keto-3-deoxygluconokinase; D000446:Aldehyde-Lyases; C021468:2-keto-3-deoxy-D-glucarate aldolase; D005947:Glucose; D005690:Galactose