title: Induction and characterization of adventitious roots directly from the explants of Panax notoginseng.
abstract: Adventitious roots from leafstalks and lateral roots were obtained directly from explants of Panax notoginseng. The lateral root explants were more sensitive to the induction of adventitious roots using indole-3-butyric acid. HPLC analysis of saponins extracted from the adventitious roots indicated that several protopanaxatriol saponins were present but ginsenoside Rd was missing, compared with the saponins extracted from the raw herbs. The dry weight of primary adventitious root culture of Panax notoginseng increased 5.25 times during multiplication in a classical shaking-flask system, suggesting that it is a culture system with great potential for scale-up.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, 200030, Shanghai, China.
authors.forename: Xianfu
authors.initials: X
authors.lastname: Gao
authors.forename: Chunbao
authors.initials: C
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Gao
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Qiu
authors.forename: Yongyu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Peigen
authors.initials: P
authors.lastname: Xiao
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D007211:Indoles / Q000494:pharmacology; D005894:Panax / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D012503:Saponins / Q000096:biosynthesis*
chemical_list: D007211:Indoles; D012503:Saponins; C014612:indolebutyric acid