title: Tickborne pathogen detection, Western Siberia, Russia.
abstract: Ixodes persulcatus (n = 125) and Dermacentor reticulatus (n = 84) ticks from Western Siberia, Russia, were tested for infection with Borrelia, Anaplasma/Ehrlichia, Bartonella, and Babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. I. persulcatus ticks were infected with Borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), Anaplasma phagocytophilum (2.4% +/- 1.4%), Ehrlichia muris (8.8% +/- 2.5%), and Bartonella spp. (37.6% +/- 4.3%). D. reticulatus ticks contained DNA of B. burgdorferi sensu lato (3.6% +/- 2.0%), Bartonella spp. (21.4% +/- 4.5%), and Babesia canis canis (3.6% +/- 2.0%). Borrelia garinii, Borrelia afzelii, and their mixed infections were observed among I. persulcatus, whereas B. garinii NT29 DNA was seen in samples from D. reticulatus. Among the I. persulcatus ticks studied, no Babesia spp. were observed, whereas B. canis canis was the single subspecies found in D. reticulatus.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch--Russian Academy of Sciences, Lavrentyev's Avenue 8, 630090 Novosibirsk, Russia.
authors.forename: Vera A
authors.initials: VA
authors.lastname: Rar
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Fomenko
authors.forename: Andrey K
authors.initials: AK
authors.lastname: Dobrotvorsky
authors.forename: Natalya N
authors.initials: NN
authors.lastname: Livanova
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Rudakova
authors.forename: Evgeniy G
authors.initials: EG
authors.lastname: Fedorov
authors.forename: Vadim B
authors.initials: VB
authors.lastname: Astanin
authors.forename: Olga V
authors.initials: OV
authors.lastname: Morozova
mesh_terms: D041081:Anaplasma phagocytophilum / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D003870:Dermacentor / Q000382:microbiology / Q000469:parasitology; D004536:Ehrlichia / Q000235:genetics / Q000302:isolation & purification; D018884:Ixodes / Q000382:microbiology / Q000469:parasitology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA; D012800:Siberia / Q000453:epidemiology; D017282:Tick-Borne Diseases / Q000382:microbiology* / Q000469:parasitology*
chemical_list: D004269:DNA, Bacterial; D016054:DNA, Protozoan