title: Identification of culturable bacteria present in haemodialysis water and fluid.
abstract: Water used to prepare haemodialysis fluid is not sterile, and its microbiological control is important for the prevention of haemodialysis-associated illness. Bacterial populations inhabiting a distribution system for haemodialysis water were studied over an 18-month period. 203 planktonic bacteria isolated on R2A medium were identified by restriction analysis and sequencing of 16S rRNA gene. A diverse bacterial community was detected, containing predominantly Gram-negative members of the Alphaproteobacteria and Betaproteobacteria, as well as representatives of the genus Mycobacterium. Ecological and clinical consequences are discussed: bacteria from the genera Novosphingobium, Pseudomonas and Sphingomonas have been described in the build-up of biofilms, and others like Acinetobacter, Mycobacterium or Brevibacterium may represent a health risk to patients under haemodialysis treatment.
authors.affiliation: Area Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), Palma de Mallorca, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Gomila
authors.forename: Joan
authors.initials: J
authors.lastname: Gascó
authors.forename: Antonio
authors.initials: A
authors.lastname: Busquets
authors.forename: José
authors.initials: J
authors.lastname: Gil
authors.forename: Rosario
authors.initials: R
authors.lastname: Bernabeu
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Buades
authors.forename: Jorge
authors.initials: J
authors.lastname: Lalucat
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D016000:Cluster Analysis; D015169:Colony Count, Microbial; D016172:DNA Fingerprinting; D017931:DNA Primers; D015312:Hemodialysis Solutions / Q000032:analysis*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D017931:DNA Primers; D015312:Hemodialysis Solutions; D012336:RNA, Ribosomal, 16S