title: Gamma-irradiation induced apoptosis in peritoneal macrophages by oxidative stress. Implications of antioxidants in caspase mitochondrial pathway.
abstract: The in vivo and in vitro development of apoptosis induced by gamma-irradiation was studied in mouse peritoneal macrophages. The apoptosis index was measured by fluorescence microscopy and DNA electrophoresis. In vivo apoptosis was greatest eight days after 8 Gy total body gamma-irradiation. A DNA ladder electrophoretic pattern was only observed in the gamma-irradiated group. The participation of reactive oxygen species in apoptosis induction was investigated by pretreating mice with the antioxidants superoxide dismutase, catalase, vitamin E or lipopolysaccharide before gamma-irradiation. Measurement of serum lipoperoxides showed oxidative stress in the gamma-irradiated mice and the protection given by the antioxidants. These results were confirmed using in vitro cultures of peritoneal macrophages: gamma-irradiated groups and antioxidant-pretreated gamma-irradiation groups showed results similar to those observed with in vivo irradiation. A loss of mitochondrial membrane potential (delta psi(m)) was also observed by microscopy in the gamma-irradiated cell cultures. Experiments with caspase inhibitors confirmed the participation of caspase 3 and caspase 9.
authors.affiliation: Centro de Investigación Biomédica de Occidente, IMSS, Guadalajara, Mexico.
authors.forename: Georgina
authors.initials: G
authors.lastname: Hernández-Flores
authors.forename: Piedad C
authors.initials: PC
authors.lastname: Gómez-Contreras
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Domínguez-Rodríguez
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Lerma-Díaz
authors.forename: Pablo César
authors.initials: PC
authors.lastname: Ortíz-Lazareno
authors.forename: Ramon
authors.initials: R
authors.lastname: Cervantes-Munguía
authors.forename: José Ernesto
authors.initials: JE
authors.lastname: Sahagún-Flores
authors.forename: Simone
authors.initials: S
authors.lastname: Orbach-Arbouys
authors.forename: Daniel
authors.initials: D
authors.lastname: Scott-Algara
authors.forename: Alejandro
authors.initials: A
authors.lastname: Bravo-Cuellar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects*; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism*; D005720:Gamma Rays; D007425:Intracellular Membranes / Q000502:physiology / Q000528:radiation effects; D008054:Lipid Peroxides / Q000097:blood; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D008297:Male; D008564:Membrane Potentials / Q000502:physiology / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D061945:Caspase Inhibitors; D008054:Lipid Peroxides; D013482:Superoxide Dismutase; D020169:Caspases