Comparison of immunohistochemistry and two rapid tests for detection of abnormal prion protein in different brain regions of sheep with typical scrapie.
One of the "gold standard" techniques for postmortem confirmation of scrapie diagnosis in sheep and goats is immunohistochemical examination of brain tissue. Active surveillance for scrapie is mainly performed by rapid diagnostic tests on the basis of postmortem immunochemical detection of prion protein (PrP) in the obex tissue. The aim of this study was to determine the performance of 2 rapid tests, Prionics-Check LIA (a chemiluminescence sandwich enzyme-linked immunosorbent assay) and Prionics-Check Western blot for scrapie diagnosis when applied to brain areas other than the obex, in comparison with the recognized immunohistochemistry. Prion protein was detected in the obex, cervical spinal cord, and thalamus from all the scrapie-positive sheep by the 3 tests. Western blot and LIA were negative in other areas of the brain, although weak immunohistochemical staining was detected. The results show that the 2 rapid tests studied may detect PrP in brain areas other than the obex, although with a lower sensitivity than immunohistochemistry when there is minimal PrP deposition.
Bolea
Rosa
R
National Reference Centre for TSE, Facultad de Veterinaria, Zaragoza, Spain.
Monleón
Eva
E
Schiller
Irene
I
Raeber
Alexander J
AJ
Acín
Cristina
C
Monzón
Marta
M
Martín-Burriel
Inmaculada
I
Struckmeyer
Thomas
T
Oesch
Bruno
B
Badiola
Juan José
JJ
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D011328:Prions / Q000302:isolation & purification*; D012608:Scrapie / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D012756:Sheep
D011328:Prions