title: Interleukin-11, IL-11 receptoralpha and leukemia inhibitory factor are dysregulated in endometrium of infertile women with endometriosis during the implantation window.
abstract: Interleukin (IL)-11 is essential for embryo implantation in the mouse and evidence suggests it has a role in implantation in humans. This study has evaluated immunoreactive IL-11, IL-11 receptor (R) alpha and leukemia inhibitory factor (LIF) in endometrium of infertile women with endometriosis (I/E) and normal fertile women (controls) during the implantation window. Endometrial biopsies from I/E (N = 7) were timed from the LH surge and were post-ovulatory days (POD) 5-10. Control biopsies (N = 8) from women were between days 19 and 24 of the menstrual cycle. Staining intensity of IL-11, IL-11Ralpha and LIF evaluated using semi-quantitative immunohistochemistry scores. Immunoreactive IL-11, IL-11Ralpha and LIF were present predominantly in glandular epithelium, while luminal epithelium showed patchy staining. All controls stained positively for IL-11, IL-11Ralpha and LIF in glandular epithelium. IL-11 and IL-11Ralpha staining was absent from glandular epithelium in cohorts of I/E. LIF staining intensity in glandular epithelium was significantly lower in I/E compared to controls. The results suggest that reduced endometrial IL-11 and/or LIF may contribute to infertility in some endometriotic women.
authors.affiliation: Prince Henry's Institute of Medical Research, P.O. Box 5152, Clayton, Vic. 3168, Australia. evdokia.dimitriadis@phimr.monash.edu.au
authors.forename: Evdokia
authors.initials: E
authors.lastname: Dimitriadis
authors.forename: Chelsea
authors.initials: C
authors.lastname: Stoikos
authors.forename: Martyn
authors.initials: M
authors.lastname: Stafford-Bell
authors.forename: Ian
authors.initials: I
authors.lastname: Clark
authors.forename: Premila
authors.initials: P
authors.lastname: Paiva
authors.forename: Gabor
authors.initials: G
authors.lastname: Kovacs
authors.forename: Lois A
authors.initials: LA
authors.lastname: Salamonsen
mesh_terms: D000328:Adult; D010064:Embryo Implantation / Q000276:immunology; D004715:Endometriosis / Q000276:immunology*; D004717:Endometrium / Q000737:chemistry / Q000276:immunology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007247:Infertility, Female / Q000276:immunology*; D017370:Interleukin-11 / Q000378:metabolism*; D053703:Interleukin-11 Receptor alpha Subunit; D015850:Interleukin-6 / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor; D011247:Pregnancy; D018123:Receptors, Interleukin / Q000378:metabolism*; D053702:Receptors, Interleukin-11; D014591:Uterine Diseases / Q000276:immunology
chemical_list: C508428:IL11RA protein, human; C508429:Il11ra1 protein, mouse; C508431:Il11ra2 protein, mouse; D017370:Interleukin-11; D053703:Interleukin-11 Receptor alpha Subunit; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11