[Experimental study on expression of the purine P2Y2 receptor in rat nerve system].
OBJECTIVE
To study the distribution of P2Y2 receptor in spine cord, dorsal root ganglia and sciatic nerve in rat, and to provide the basis for clarifying the mechanism of the effect of adenosine triphosphate (ATP) on the peripheral nerve regeneration.


METHODS
Six specimens of the spine cord, dorsal root ganglia and sciatic nerve from SD rats were fixed rapidly in 4% paraformaldehyde which included DEPC, imbedded by paraffin and made into ultrathin section. According to the sequence of P2Y2 receptor's gene, DNA needle was adopted to detect the distribution of P2Y2 receptor by hybridization technique in section under the light microscope after they had been stained in NBT liquid(50 mg/ml) and BCIP liquid (75 mg/ml). In the control group, the ultrathin section was only covered with hybridization buffer solution. The result of staining was observed.


RESULTS
Hybridization in section showed that P2Y2 receptor was distributed mainly in the anterior horn cell of spine cord gray matter and Schwann cell of the dorsal root ganglia. No P2Y2 receptor was observed in the sciatic nerve of both groups.


CONCLUSIONS
P2Y2 receptor is located mainly in the spine cord and the dorsal root ganglia. Extracellular ATP can affect the cell of spine cord, dorsal root ganglia through P2Y2 receptor.
Yu
Yadong
Y
Department of Hand Surgery, the Third Affiliated Hospital of Hebei Medical University, Shijiazhuang Hebei, P. R. China. yuyadong0626@126.com
Guo
Qing
Q
Hong
Guang Xiang
GX
D000818:Animals; D005727:Ganglia, Spinal / Q000378:metabolism; D017403:In Situ Hybridization; D009420:Nervous System / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011983:Receptors, Purinergic / Q000235:genetics / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism
D012333:RNA, Messenger; D011983:Receptors, Purinergic