title: Global Analysis of the Hortaea werneckii proteome: studying steroid response in yeast.
abstract: The response of the halophilic black yeast Hortaea werneckii to the steroid hormone progesterone has been studied at the protein level using fluorescent two-dimensional differential gel electrophoresis (2D-DIGE) technology in combination with mass spectrometry. Data on protein identification from this study reveal molecular mechanisms of the response to progesterone. In particular, the overexpression of Pck2 and Pac2 in the stimulated cells indicates the interactions of progesterone with the cell growth and reproduction signaling pathways.
authors.affiliation: Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912, USA.
authors.forename: Maja
authors.initials: M
authors.lastname: Matis
authors.forename: Marija
authors.initials: M
authors.lastname: Zakelj-Mavric
authors.forename: Jasna
authors.initials: J
authors.lastname: Peter-Katalinić
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000737:chemistry*; D005786:Gene Expression Regulation; D013058:Mass Spectrometry; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D011374:Progesterone / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence; D013256:Steroids / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D005656:Fungal Proteins; D011506:Proteins; D020543:Proteome; D013256:Steroids; D011374:Progesterone