Thermodynamic regulation of human short-chain acyl-CoA dehydrogenase by substrate and product binding.
Human short-chain acyl-CoA dehydrogenase (hSCAD) catalyzes the first matrix step in the mitochondrial beta-oxidation cycle for substrates with four and six carbons. Previous studies have shown that the act of substrate/product binding induces a large enzyme potential shift in acyl-CoA dehydrogenases. The objective of this work was to examine the thermodynamic regulation of this process through direct characterization of the electrochemical properties of hSCAD using spectroelectrochemical methodology. A large amount of substrate activation was observed in the enzymatic reaction of hSCAD (+33 mV), the greatest magnitude measured in any acyl-CoA dehydrogenase to date. To examine the role of the substrate as well as the product in electron transfer by hSCAD, a catalytic base mutation (E368Q) was constructed. The E368Q mutation inactivates the reductive and oxidative pathways such that the individual effects of substrate and product binding on the redox potential can be investigated. Optimal substrate (butyryl-CoA) was seen to shift the flavin redox potential slightly more positive (+38 mV) than did optimal product (crotonyl-CoA) (+31 mV), a finding opposite of that observed in another short-chain enzyme, bacterial SCAD. These results indicate that substrate redox activation occurs in hSCAD leading to a large enzyme midpoint potential shift. Substrate binding in hSCAD appears to make a larger contribution than does product to thermodynamic modulation.
Saenger
Amy K
AK
Department of Chemistry, University of Minnesota, 207 Pleasant Street, SE Kolthoff and Smith Halls, Minneapolis, Minnesota 55455, USA.
Nguyen
Tien V
TV
Vockley
Jerry
J
Stankovich
Marian T
MT
D044926:Butyryl-CoA Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004563:Electrochemistry; D004789:Enzyme Activation; D005415:Flavins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008024:Ligands; D010084:Oxidation-Reduction; D017354:Point Mutation; D011485:Protein Binding; D013816:Thermodynamics
D005415:Flavins; D008024:Ligands; D044926:Butyryl-CoA Dehydrogenase