Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by Cladosporium sphaerospermum isolated from an aged PAH contaminated soil.
The ability of a Deuteromycete fungus, Cladosporium sphaerospermum, previously isolated from soil of an aged gas manufacturing plant, to degrade polycyclic aromatic hydrocarbons was investigated. This strain was able to degrade PAHs in non-sterile soils (average 23%), including high molecular weight PAHs, after 4 weeks of incubation. In a microcosm experiment, PAH depletion was clearly correlated to fungal establishment. In liquid culture, this strain degraded rapidly benzo(a)pyrene during its early exponential phase of growth (18% after 4 days of incubation). Among extracellular ligninolytic enzyme activities tested, only laccase activity was detected in liquid culture in the absence or in presence of benzo(a)pyrene. C. sphaerospermum might be a potential candidate for an effective bioremediation of aged PAH-contaminated soils.
Potin
Olivier
O
Université du Littoral Côte d'Opale, 50 rue Ferdinand Buisson, BP 699, F-62228 Calais Cédex, France.
Veignie
Etienne
E
Rafin
Catherine
C
D001673:Biodegradation, Environmental; D002963:Cladosporium / Q000502:physiology*; D004787:Environmental Pollution / Q000517:prevention & control; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*
D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants