Effects of multipurpose contact lens solutions on the protein composition of the tear film.
OBJECTIVE
To analyze the influence of multipurpose contact lens cleaning solutions on tear proteins. Changes in tear film protein profiles of contact lens wearers who used several marketed brands of multipurpose contact lens care solutions, were assessed by ProteinChip analysis.


METHODS
Three studies were conducted. Study I was a comparison of Complete and OptiFree multipurpose solutions. Study II was a study with Complete Moisture Plus solution, Study II was a comparison of Renu and Solocare contact lens solutions. Wearers of soft contact lenses were assigned to use the contact lens care solutions for 4 weeks. Non-contact lens wearing patients were used as controls. Tear samples of each participant were analyzed with the ProteinChip (SELDI-TOF) system. Multivariate statistical analysis and artificial neural networks were used to determine the tear protein profiles of each study group.


RESULTS
Before starting the use of the solutions, the tear protein composition in all contact lens wearers deviated from the tear composition of the non-contact lens wearing controls. After 4 weeks of using the different care regimens, the tear protein composition of the patients using Complete or Complete Moisture Plus solutions tended to move toward that of the non-contact lens wearing controls. The tear protein composition of patients using the OptiFree, Renu or Solocare solutions did not undergo a measureable change in the protein level.


CONCLUSIONS
The ProteinChip system can analyze protein profiles for large-scale applications as in clinical studies. Two multipurpose solutions, Complete and Complete Moisture Plus, demonstrated a beneficial effect on the tear proteins in contact lens wearers.
Grus
Franz H
FH
Department of Ophthalmology, University of Mainz, Germany. grus@tearfilm.net
Kramann
C
C
Bozkurt
N
N
Wiegel
N
N
Bruns
K
K
Lackner
N
N
Pfeiffer
Norbert
N
D018639:Contact Lens Solutions / Q000494:pharmacology*; D003261:Contact Lenses; D005136:Eye Proteins / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D012030:Refractive Errors / Q000534:rehabilitation; D013666:Tears / Q000187:drug effects* / Q000378:metabolism
D018639:Contact Lens Solutions; D005136:Eye Proteins; C042434:tear proteins