title: Production of a recombinant bacterial lipoprotein in higher plant chloroplasts.
abstract: Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)-the basic constituent of the first generation monovalent human vaccine against Lyme disease. The recombinant OspA exhibits a lipid modification typical for bacteria and induced protective antibodies in mice, demonstrating that functionally active bacterial lipoproteins can be processed in plants.
authors.affiliation: Department of Pharmaceutical Biology, Julius-von-Sachs-Institut für Biowissenschaften, Julius-Maximilians Universität Wuerzburg, Julius-von-Sachs-Platz 2, 97082 Wuerzburg, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Glenz
authors.forename: Bernadette
authors.initials: B
authors.lastname: Bouchon
authors.forename: Thomas
authors.initials: T
authors.lastname: Stehle
authors.forename: Reinhard
authors.initials: R
authors.lastname: Wallich
authors.forename: Markus M
authors.initials: MM
authors.lastname: Simon
authors.forename: Heribert
authors.initials: H
authors.lastname: Warzecha
mesh_terms: D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001428:Bacterial Vaccines; D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
chemical_list: D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; C072045:OspA protein; D011994:Recombinant Proteins