Gene expression in the cell cycle of human T-lymphocytes: II. Experimental determination by DNASER technology.
Human lymphocytes gene expression before and after PHA stimulation is monitored by DNASER technology, a novel bioinstrumentation entirely constructed in our laboratories as previously reported. The validity of the DNASER measurements is confirmed by standard fluorescence microscopy equipped with CCD. The human lymphocytes gene expression here experimentally probed using commercially available DNA microarrays such as Human Starter, appears compatible both with independent bioinformatic prediction and with existing experimental data, pointing to MYC as the key gene in the G0-G1 transition induced by PHA in resting lymphocytes. It does not escape our notice that in cell biology and cancer research DNASER technology based on microarray constructed with few leader genes identified from bioinformatics represents a meaningful cost-effective route alternative to massive frequently misleading molecular genomics.
Nicolini
Claudio
C
Nanoworld Institute and Biophysics Division, University of Genova, Corso Europa 30, 16132 Genova (Italy). manuscript@ibf.unige.it
Spera
Rosanna
R
Stura
Enrico
E
Fiordoro
Stefano
S
Giacomelli
Luca
L
D002453:Cell Cycle; D019295:Computational Biology; D015870:Gene Expression; D005786:Gene Expression Regulation; D016259:Genes, myc; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013601:T-Lymphocytes / Q000378:metabolism*; D014158:Transcription, Genetic