Degradation of cyclins D in pseudorabies virus (PRV) infected proliferating cells.
The pseudorabies virus code for an ICP0 protein which is half the size of the HSV1 ICP0 protein. In this work, we made the assumption that some function might have been lost in the ICP0 from PRV. One function attributed to the ICP0 from HSV1 was the stabilization of cyclins D. We then looked at the stability of these cyclins during the lytic infection with the PRV. Our results show that cyclins D are not stabilized during infection with the PRV. These results are in accord with recent data from the literature.
Blanchard
Yannick
Y
Laboratoire de Génétique Virale et Biosécurité, Afssa. BP53, Fr-22440 Ploufragan, France. y.blanchard@afssa.fr
Dory
Daniel
D
Briand
François Xavier
FX
Félix
Hélène
H
Jestin
André
A
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002478:Cells, Cultured; D019926:Cyclin B / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism*; D005434:Flow Cytometry / Q000662:veterinary; D005453:Fluorescence; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism; D008970:Molecular Weight; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism; D014774:Virulence / Q000235:genetics
D019926:Cyclin B; D016213:Cyclins; D017874:Immediate-Early Proteins; D019938:Cyclin D1; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1