title: Novel primers reveal wider diversity among marine aerobic anoxygenic phototrophs.
abstract: Aerobic anoxygenic phototrophic bacteria (AAnPs) were previously proposed to account for up to 11% of marine bacterioplankton and to potentially have great ecological importance in the world's oceans. Our data show that previously used primers based on the M subunit of anoxygenic photosynthetic reaction center genes (pufM) do not comprehensively identify the diversity of AAnPs in the ocean. We have designed and tested a new set of pufM-specific primers and revealed several new AAnP variants in environmental DNA samples and genomic libraries.
authors.affiliation: Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel.
authors.forename: Natalya
authors.initials: N
authors.lastname: Yutin
authors.forename: Marcelino T
authors.initials: MT
authors.lastname: Suzuki
authors.forename: Oded
authors.initials: O
authors.lastname: Béjà
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001483:Base Sequence; D044822:Biodiversity; D017931:DNA Primers; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010788:Photosynthesis; D010802:Phylogeny; D016992:Rhodobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*
chemical_list: D017931:DNA Primers