title: Optimization of aromatic side chain size complementarity in the hydrophobic core of a designed coiled-coil.
abstract: The coiled-coil structure plays an important roles, especially in protein assembly. Previously we constructed AAB-type heterotrimeric coiled-coils by manipulating the packing in the hydrophobic core using Trp and Ala residues, where one Trp and two Ala residues were placed in the hydrophobic core instead of three Ile residues. To optimize the packing complementarity in the hydrophobic core, we investigated the effects of introducing various aromatic amino acids on the formation of an AAB-type heterotrimeric coiled-coil, by circular dichroism, thermal stability, and nuclear magnetic resonance (NMR) studies. We found that the Phe residue was more suitable for heterotrimeric coiled-coil formation than the Trp residue, when combined with two Ala residues, whereas the Tyr and His residues did not induce the coiled-coil structure efficiently.
authors.affiliation: Department of Material Sciences, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-chou, Nagoya 466-8555, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakurai
authors.forename: T
authors.initials: T
authors.lastname: Mizuno
authors.forename: H
authors.initials: H
authors.lastname: Hiroaki
authors.forename: J-I
authors.initials: JI
authors.lastname: Oku
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D024322:Amino Acids, Aromatic; D002850:Chromatography, Gel; D002942:Circular Dichroism; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D020836:Protein Structure, Quaternary
chemical_list: D000596:Amino Acids; D024322:Amino Acids, Aromatic; D010455:Peptides