title: Recognition of SCCmec types according to typing pattern determined by multienzyme multiplex PCR-amplified fragment length polymorphism analysis of methicillin-resistant Staphylococcus aureus.
abstract: Multienzyme multiplex PCR-amplified fragment length polymorphism (ME-AFLP) typing is a reliable and simple method for typing of bacterial species. In this study we analyzed two well-documented strain collections of Staphylococcus aureus and compared ME-AFLP typing results with results of various other typing methods. The discriminatory power of ME-AFLP was found comparable to pulsed-field gel electrophoresis, and typing results were highly concordant. ME-AFLP typing presents a suitable method for prescreening of large strain collections. Furthermore, the obtained typing patterns were found to cluster according to the staphylococcal cassette chromosome mec types of the strains.
authors.affiliation: Laboratory of Medical Microbiology, St. Elisabeth Hospital, PO Box 747, 5000 AS Tilburg, The Netherlands. a.vd.zee@elisabeth.nl
authors.forename: Anneke
authors.initials: A
authors.lastname: van der Zee
authors.forename: Max
authors.initials: M
authors.lastname: Heck
authors.forename: Marcel
authors.initials: M
authors.lastname: Sterks
authors.forename: Airien
authors.initials: A
authors.lastname: Harpal
authors.forename: Emile
authors.initials: E
authors.lastname: Spalburg
authors.forename: Leonard
authors.initials: L
authors.lastname: Kazobagora
authors.forename: Wim
authors.initials: W
authors.lastname: Wannet
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D016106:Methicillin Resistance; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins