title: [A three-dimension in vitro model for angiogenesis of hemangioma].
abstract: OBJECTIVE
  To create a three dimension (3D) in vitro model for angiogenesis of hemangioma.
  
  
  METHODS
  The fragment of hemangioma specimen was embedded in fibrin gel to set up the three-dimension (3D) in vitro model for angiogenesis of hemangioma.
  
  
  RESULTS
  In the model, microvessels grew out from the tissue fragments at the 2nd to 3rd day after culture, and at the 8th to 9th day a compact network of microvessels come into being, then tending to be stationary. The compact network around the tissue fragment was confirmed to be blood vessels by immunohistochemistry and electron microscopy.
  
  
  CONCLUSIONS
  This model helps to study the mechanism of hemangioma angiogenesis and investigate the drugs of anti-angiogenesis.
authors.affiliation: The Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Cheng-Hong
authors.initials: CH
authors.lastname: Jiang
authors.forename: Fu-Lian
authors.initials: FL
authors.lastname: Zhuang
authors.forename: Ba-Rui
authors.initials: BR
authors.lastname: Huang
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Guo
authors.forename: Yi-De
authors.initials: YD
authors.lastname: Xie
authors.forename: Ya-Kuan
authors.initials: YK
authors.lastname: Zhou
authors.forename: Biao
authors.initials: B
authors.lastname: Wang
authors.forename: Wen-lie
authors.initials: WL
authors.lastname: Chen
mesh_terms: D002478:Cells, Cultured; D004730:Endothelium, Vascular; D006391:Hemangioma; D006801:Humans; D008955:Models, Cardiovascular; D009389:Neovascularization, Pathologic