The cytolethal distending toxin among Helicobacter pullorum strains from human and poultry origin.
Helicobacter pullorum has been associated with diarrhoea, gastroenteritis and liver disease in humans and with hepatitis and enteritis in poultry. The purpose of the present study was to examine whether cytolethal distending toxin was present among 10 poultry and three human H. pullorum isolates and whether a different level of cytolethal distending toxin activity was noted. A PCR assay was performed to detect the cdtB gene. In addition, epithelial Hep-2 cells inoculated with sonicate from all strains were observed microscopically and DNA analysis of these cells was done by flow cytometry. All H. pullorum isolates harboured the cdtB gene, but functional cytolethal distending toxin activity was only demonstrated in the human H. pullorum strain CCUG 33839. A significant number of cells treated with sonicate from this strain were enlarged. The nuclei were distended proportionally. Giant cells and multinucleated cells were observed as well. In addition, stress fibers accumulated. DNA analysis by flow cytometry revealed 31.0% of these cells at the S/G2 stage of the cell cycle. The tested poultry and human H. pullorum isolates all possess the cdtB gene, but under the circumstances adopted in this study only the human strain CCUG 33839 seems to show biological activity typically for CDT in vitro.
Ceelen
Liesbeth M
LM
Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. liesbeth.ceelen@UGent.be
Haesebrouck
Freddy
F
Favoreel
Herman
H
Ducatelle
Richard
R
Decostere
Annemie
A
D008841:Actin Cytoskeleton / Q000473:pathology; D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D016123:Campylobacter jejuni; D002452:Cell Count; D045744:Cell Line, Tumor; D004247:DNA / Q000032:analysis; D004847:Epithelial Cells / Q000737:chemistry / Q000473:pathology; D005434:Flow Cytometry / Q000379:methods; D016998:Helicobacter / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D016481:Helicobacter Infections / Q000382:microbiology* / Q000503:physiopathology; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011200:Poultry; D011201:Poultry Diseases / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D001427:Bacterial Toxins; C067044:cytolethal distending toxin; D004247:DNA