title: Praziquantel and albendazole damaging action on in vitro developing Mesocestoides corti (Platyhelminthes: Cestoda).
abstract: Parasitic flatworms present several steps of body architecture rearrangement during their fast transition from one developmental stage to another, which are, at least in part, responsible for their evasion from host immune response. Besides, different developmental stages present different degrees of susceptibility to drug action, and the identification of more susceptible stages is of importance for the definition of therapeutical approaches. Mesocestoides corti (syn. Mesocestoides vogae) is considered a good model to study cestode biology because it can be easily manipulated both in vivo and in vitro and due to its relatively close relationship to cestodes of medical relevance, such as those from genera Echinococcus or Taenia. We have analyzed the damaging action of two broad spectrum anthelmintic drugs (praziquantel and albendazole) throughout the in vitro strobilization process of M. corti in order to identify developmental stages or body structures more susceptible to these drugs. Tetrathyridia (larval stage) and segmented-induced worms were cultivated and treated with praziquantel and albendazole. Whole mounted samples, taken from different developmental stages, were fixed and stained with fluorophore-labeled WGA lectin and phalloidin for the analysis of tegument and muscles, respectively. Confocal laser scanning microscopy was used to identify anatomical changes and lesions caused by each anthelmintic drug in a 3D view. We demonstrated that both praziquantel and albendazole cause extensive tissue damage, especially on tegument, and that adult forms were the most susceptible to drug exposure.
authors.affiliation: Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, 91501-970, Porto Alegre, Brazil.
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Markoski
authors.forename: Edvaldo S
authors.initials: ES
authors.lastname: Trindade
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Cabrera
authors.forename: Alice
authors.initials: A
authors.lastname: Laschuk
authors.forename: Norbel
authors.initials: N
authors.lastname: Galanti
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Zaha
authors.forename: Helena B
authors.initials: HB
authors.lastname: Nader
authors.forename: Henrique B
authors.initials: HB
authors.lastname: Ferreira
mesh_terms: D015766:Albendazole / Q000494:pharmacology*; D000825:Animal Structures / Q000187:drug effects; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D000923:Anticestodal Agents / Q000494:pharmacology; D003470:Culture Media; D008645:Mesocestoides / Q000187:drug effects* / Q000254:growth & development* / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000379:methods; D009132:Muscles / Q000187:drug effects; D011223:Praziquantel / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000871:Anthelmintics; D000923:Anticestodal Agents; D003470:Culture Media; D011223:Praziquantel; D015766:Albendazole