Rapid detection of subtype-selective nuclear hormone receptor binding with bacterial genetic selection.
Subtype-selective nuclear hormone receptor modulators could potentially allow the development of valuable tissue-specific therapeutics. A simple biosensor that allows subtype-specific nuclear hormone receptor binding to be reflected by the growth phenotype of Escherichia coli cells has been constructed. This system will potentially enable the facile detection or evolution of subtype-selective hormone analogues.
Skretas
Georgios
G
Department of Chemical Engineering, Princeton University, Engineering Quadrangle, Olden St., Princeton, NJ 08544, USA.
Wood
David W
DW
D001422:Bacterial Adhesion; D015374:Biosensing Techniques; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D047628:Estrogen Receptor alpha / Q000502:physiology*; D047629:Estrogen Receptor beta / Q000502:physiology*; D004967:Estrogens / Q000032:analysis; D008024:Ligands; D010641:Phenotype
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D008024:Ligands