title: Transient expression of fast troponin C transcripts in embryonic quail heart.
abstract: Most myofibrillar proteins, including troponin I and troponin T subunits of troponin complex, undergo developmental stage-specific isoform transitions in vertebrate heart before attaining adult contractile and regulatory characteristics. Only the cardiac/slow skeletal muscle type isoform of troponin C, however, has been shown to be expressed in both adult and developing heart. The changes in troponin C could be functionally important as the TnC isoforms vary in their affinities for Ca(2+). For example, fast troponin C has two Ca(2+) binding sites while slow/cardiac troponin C has a single regulatory site. This study demonstrates the co-expression of both fast and slow transcripts of troponin C in not only quail embryonic skeletal muscle but also embryonic heart using two different analytical techniques of polymerase chain reaction and in situ hybridisation procedure. Fast troponin C expression in the quail heart using in situ hybridisation procedure was first observed at embryonic day 3, with maximum expression at day 5 after which its level in the developing heart was gradually down regulated. In situ hybridisation staining of sections at these developmental stages demonstrated the expression of both fast and slow transcripts of troponin C in all cardiomyocytes.
authors.affiliation: Department of Basic Sciences, The Royal Veterinary College, University of London, Royal College Street, NW1 OTU London, UK.
authors.forename: A
authors.initials: A
authors.lastname: Stoutamyer
authors.forename: G K
authors.initials: GK
authors.lastname: Dhoot
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology* / Q000254:growth & development; D017403:In Situ Hybridization; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000196:embryology* / Q000254:growth & development; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D011784:Quail / Q000196:embryology* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019209:Troponin C / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D019209:Troponin C; D002118:Calcium