Induction of heparanase gene expression in ventricular myocardium of rats with isoproterenol-induced cardiac hypertrophy.
Gene expression of heparanase, matrix metalloproteinases (MMP)-2 and MMP-9 were examined in ventricles after chronic treatment with isoproterenol (ISO) induced cardiac hypertrophy in rats. Rats were treated with ISO (4 mg/kg, intraperitoneal) twice daily for 4 d. Ventricle weight of the heart and the ventricle weight/body weight ratio were increased respectively by 22% and 25% compared with control rats. Histology showed considerable cardiomyocyte hypertrophy in the ISO-treated rats in comparison to control rats. Northern blot hybridization revealed that heparanase and MMP-2 gene transcripts increased significantly in the ventricles of ISO-treated rats, whereas MMP-9 gene expression was not induced. Thus, heparanase and MMP-2 gene expressions are induced in the ventricle after chronic treatment with ISO, indicating that they might play an important role in development of ISO-induced cardiac hypertrophy.
Kizaki
Keiichiro
K
Department of Veterinary Pharmacology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan. kizaki@iwate-u.ac.jp
Okada
Muneyoshi
M
Ito
Ryoichi
R
Yoshioka
Kazuki
K
Hashizume
Kazuyoshi
K
Mutoh
Ken-ichiro
K
Hara
Yukio
Y
D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D006332:Cardiomegaly / Q000139:chemically induced / Q000201:enzymology*; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D004790:Enzyme Induction / Q000187:drug effects; D005109:Extracellular Matrix; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005966:Glucuronidase / Q000096:biosynthesis*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D017379:Hypertrophy, Left Ventricular / Q000201:enzymology; D017380:Hypertrophy, Right Ventricular / Q000201:enzymology; D007274:Injections, Intraperitoneal; D007545:Isoproterenol; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D009206:Myocardium / Q000201:enzymology*; D032383:Myocytes, Cardiac / Q000201:enzymology; D018075:RNA, Complementary / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000099175:Heparanase
D005966:Glucuronidase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D007545:Isoproterenol; D020778:Matrix Metalloproteinase 2; D018075:RNA, Complementary; D000099175:Heparanase