Completion of the genome sequence of Lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus.
We completed the genome sequence of Lettuce necrotic yellows virus (LNYV) by determining the nucleotide sequences of the 4a (putative phosphoprotein), 4b, M (matrix protein), G (glycoprotein) and L (polymerase) genes. The genome consists of 12,807 nucleotides and encodes six genes in the order 3' leader-N-4a(P)-4b-M-G-L-5' trailer. Sequences were derived from clones of a cDNA library from LNYV genomic RNA and from fragments amplified using reverse transcription-polymerase chain reaction. The 4a protein has a low isoelectric point characteristic for rhabdovirus phosphoproteins. The 4b protein has significant sequence similarities with the movement proteins of capillo- and trichoviruses and may be involved in cell-to-cell movement. The putative G protein sequence contains a predicted 25 amino acids signal peptide and endopeptidase cleavage site, three predicted glycosylation sites and a putative transmembrane domain. The deduced L protein sequence shows similarities with the L proteins of other plant rhabdoviruses and contains polymerase module motifs characteristic for RNA-dependent RNA polymerases of negative-strand RNA viruses. Phylogenetic analysis of this motif among rhabdoviruses placed LNYV in a group with other sequenced cytorhabdoviruses, most closely related to Strawberry crinkle virus.
Dietzgen
Ralf G
RG
Department of Primary Industries and Fisheries, Queensland Agricultural Biotechnology Centre, Queensland Bioscience Precinct, The University of Queensland, 306 Carmody Rd, St. Lucia, Qld. 4072, Australia. ralf.dietzgen@dpi.qld.gov.au
Callaghan
Ben
B
Wetzel
Thierry
T
Dale
James L
JL
D020816:Amino Acid Motifs; D001483:Base Sequence; D023061:Gene Order; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics; D010802:Phylogeny; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D012209:Rhabdoviridae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014763:Viral Matrix Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D006023:Glycoproteins; D010750:Phosphoproteins; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase