A beta-galactose-specific lectin isolated from the marine worm Chaetopterus variopedatus possesses anti-HIV-1 activity.
A 30 kDa beta-galactose-specific lectin named CVL was isolated from the polychaete marine worm Chaetopterus variopedatus (Annelida) and its anti-HIV-1 activity in vitro was determined. Results showed that CVL inhibited cytopathic effect induced by HIV-1 and the production of viral p24 antigen. The EC(50) values were 0.0043 and 0.057 microM, respectively. Time-of-addition analysis of anti-HIV-1 activity indicated its action was at the early stage of virus replication. CVL could blocked the cell-to-cell fusion process of HIV infected and uninfected cells with an EC(50) of 0.073 microM. The inhibition of HIV-1 entry into host cells was demonstrated by using fluorescence-based real-time quantify PCR. At CVL concentration of 0.33 microM and 0.07 microM, 86% and 21% virus attachment were blocked, respectively. The anti-HIV-1 action of CVL might relate to blockade of HIV-1 entry into cells.
Wang
Jian-Hua
JH
Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan, China.
Kong
Jing
J
Li
Wei
W
Molchanova
Valentina
V
Chikalovets
Irina
I
Belogortseva
Natalia
N
Luk'yanov
Pavel
P
Zheng
Yong-Tang
YT
D000818:Animals; D019380:Anti-HIV Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D005690:Galactose / Q000737:chemistry; D037161:Galectins / Q000302:isolation & purification* / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology*; D011077:Polychaeta / Q000737:chemistry*
D019380:Anti-HIV Agents; C509558:CVL galactose-specific lectin, Chaetopterus variopedatus; D037161:Galectins; D037102:Lectins; D005690:Galactose