[The qualitative analysis and quantitative analysis of purification of salvianolic acids by macroreticular resin].
OBJECTIVE
To purify salvianolic acids by macroreticular resin,then mensurate the contents of salvianolic acids and analyse the chromatogram with HPLC.


METHODS
Make salvianolic acids with macroreticular resin; mensurate the content of Salvianolic acids with UV spestrophotometry: the control compound is protocaechuic aldehyde, and the wavelength is 281 nm. Analysis the chromatogram with HPLC, and compare the chromatogram in different technics: zorbax ODS column (4.6 mm x 250 mm, 5 microm), mobilephase: 1% aceticacid-water and methanol in different proportions, the wavelength is 281 nm.


RESULTS
The contents of salvianolic acids is 53.8%; HPLC chromatogram indicate that the method is reasonable to make salvianolic acids.


CONCLUSIONS
Determination of contents and HPLC chromatogram can control the quality of Salvianolic acids more accurately.
Fang
Xin-sheng
XS
Key Laboratory of Pharmaceutics of Chinese Material Medica, Nan Fang Medical University, Guangzhou 510515, China.
Tan
Xiao-mei
XM
D001572:Benzofurans / Q000032:analysis / Q000302:isolation & purification*; D002109:Caffeic Acids / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D007475:Ion Exchange Resins; D007773:Lactates / Q000032:analysis / Q000302:isolation & purification*; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D053630:Depsides
D001572:Benzofurans; D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D007475:Ion Exchange Resins; D007773:Lactates; C066201:salvianolic acid A; C076944:salvianolic acid B; D053630:Depsides