[Expression of A-type atrial natriuretic peptide receptor in the kidneys of renovascular hypertension rats and its implication].
OBJECTIVE
To investigate the expression of A-type atrial natriuretic peptide receptor (ANPR-A) in the kidneys of renovascular hypertension rats and evaluate the significance of the expression.


METHODS
The rat model of renovascular hypertension was produced by constricting one lateral renal artery. After the renal artery being constricted for 4 weeks and 8 weeks, the systolic BP of rats was measured with a manometer using the tail-cuff method. Then, the expression of ANPR-A was respectively detected by immunohistochemical technique in the kidneys of the two-kidney, one-clip (2K1C) rats, and the expression level of ANPR-A was semi-quantitatively measured by Mias-2000 computer image analyzer.


RESULTS
At 4 weeks after the artery-constricted operation,the expression of ANPR-A increased significantly in 2K1C hypertensive rat glomeruli and decreased significantly in renal tubules, compared with control (P<0.01), but there was no marked change in medullar collecting tubules. At 8 weeks after the artery-constricted operation, the expression of ANPR-A decreased significantly in 2K1C hypertensive rat renal tubules and medullar collecting tubules, compared with control (P<0.01); however, there was weak expression in glomeruli, and no statistically significant difference was seen when compared with control (P>0.05).


CONCLUSIONS
The expression of ANPR-A decreased significantly in kidney tissues of renovascular
Liu
Rong-Tao
RT
Biomedical Engineering Department, West China School of Preclinical and Forensic Medicine, Sichuang University, Chengdu 610041, China.
Xiao
Jing
J
Guo
Hui-Ling
HL
Qiu
Dun-Guo
DG
Yin
Hua-Hu
HH
Wang
Zheng-Rong
ZR
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D006978:Hypertension, Renovascular / Q000209:etiology / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017461:Receptors, Atrial Natriuretic Factor / Q000096:biosynthesis* / Q000235:genetics
D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor