title: [Immunohistochemical localization of leucine-rich proteoglycans in the developing periodontal tissues of mice].
abstract: OBJECTIVE
  To study the distribution and expression of fibromodulin, decorin and biglycan in developing normal periodontal tissues, so as to understand its role in periodontal tissue formation.
  
  
  METHODS
  Thirty six BALB/c mice in different developing stages were killed and their bilateral mandibular first molars with surrounding alveolar bones and gingival tissues were taken out, Power Vision two steps immunohistochemical method with anti-fibromodulin, anti-decorin and anti-biglycan was used to detect the tissue distribution and cellular localization of fibromodulin and related proteoglycans, decorin and biglycan.
  
  
  RESULTS
  Fibromodulin was strongly expressed in the subcutaneous gingival connective tissue, periodontal ligament, mainly in gingival and periodontal fibroblasts as well as their matrices. Strong expression was also noted in the area close to the interfaces of periodontal ligament-alveolar bone and periodontal ligament-cementum. Decorin was strongly expressed in the area of gingival connective tissue, periodontal ligament and the surface of alveolar bone, while biglycan was stained evidently in gingival connective tissue throughout the period of investigation, but negative in the surface of alveolar bone and osteoblasts.
  
  
  CONCLUSIONS
  Fibromodulin may interact with decorin and biglycan to regulate the network formation of gingival connective tissues and periodontal collagen fibers, and may be involved in mineralization of the alveolar bone and cementum.
authors.affiliation: Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Pi-shan
authors.initials: PS
authors.lastname: Yang
authors.forename: Ke-qing
authors.initials: KQ
authors.lastname: Pan
authors.forename: Rui-yue
authors.initials: RY
authors.lastname: Xie
authors.forename: Xue-jing
authors.initials: XJ
authors.lastname: Duan
mesh_terms: D000539:Alveolar Process / Q000166:cytology* / Q000254:growth & development; D000818:Animals; D058578:Biglycan; D058575:Decorin; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D000071235:Fibromodulin; D005881:Gingiva / Q000737:chemistry* / Q000254:growth & development; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000737:chemistry*; D010513:Periodontal Ligament / Q000737:chemistry* / Q000254:growth & development; D011509:Proteoglycans / Q000032:analysis*; D014083:Tooth Germ / Q000737:chemistry
chemical_list: C546885:Bgn protein, mouse; D058578:Biglycan; C546879:Dcn protein, mouse; D058575:Decorin; D016326:Extracellular Matrix Proteins; C000604332:Fmod protein, mouse; D011509:Proteoglycans; D000071235:Fibromodulin