title: Metabolic engineering of plant cells for biotransformation of hesperedin into neohesperidin, a substrate for production of the low-calorie sweetener and flavor enhancer NHDC.
abstract: Neohesperidin dihydrochalcone (NHDC) is a seminatural, safe, low-calorie sweetener, bitterness blocker, and flavor enhancer with unique properties and applications for the food, beverage, pharmaceutical, and animal feed industries. Current production is limited by the availability of the substrate neohesperidin, a flavonoid that accumulates to significant levels only in the inedible bitter citrus species. We propose a process to convert hesperidin, a tasteless flavonoid extracted from orange peels that are abundant byproducts of the vast orange juice industry, into neohesperidin using metabolic engineering and biotransformation via three steps: (i) extraction of hesperidin from orange peels, (ii) hydrolysis of sugar moieties, and (iii) biotransformation of hesperidin hydrolysis products into neohesperidin. We overcame the current technological bottleneck in biotransformation of hesperidin hydrolysis products into neohesperidin using metabolically engineered plant cell cultures expressing a recombinant flavanone-7-O-glucoside-2-O-rhamnosyltransferase. A small-scale production experiment established the feasibility of the proposed process.
authors.affiliation: Institute of Horticulture, The Volcani Center, ARO, P.O. Box 6, Bet-Dagan 50250, Israel.
authors.forename: Ahuva
authors.initials: A
authors.lastname: Frydman
authors.forename: Oori
authors.initials: O
authors.lastname: Weisshaus
authors.forename: David V
authors.initials: DV
authors.lastname: Huhman
authors.forename: Lloyd W
authors.initials: LW
authors.lastname: Sumner
authors.forename: Maor
authors.initials: M
authors.lastname: Bar-Peled
authors.forename: Efraim
authors.initials: E
authors.lastname: Lewinsohn
authors.forename: Robert
authors.initials: R
authors.lastname: Fluhr
authors.forename: Jonathan
authors.initials: J
authors.lastname: Gressel
authors.forename: Yoram
authors.initials: Y
authors.lastname: Eyal
mesh_terms: D002478:Cells, Cultured; D002599:Chalcone / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D047188:Chalcones; D018552:Daucus carota / Q000235:genetics; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism*; D006602:Hexosyltransferases / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology*; D011994:Recombinant Proteins / Q000378:metabolism; D013549:Sweetening Agents / Q000138:chemical synthesis; D014026:Nicotiana / Q000235:genetics
chemical_list: D047188:Chalcones; D011994:Recombinant Proteins; D013549:Sweetening Agents; C013613:neohesperidin dihydrochalcone; D002599:Chalcone; D006569:Hesperidin; D006602:Hexosyltransferases; C071540:UDP-rhamnose-flavanone-7-O-glucoside-2''-O-rhamnosyltransferase