title: Acetohydroxyacid synthase isozyme I from Escherichia coli has unique catalytic and regulatory properties.
abstract: AHAS I is an isozyme of acetohydroxyacid synthase which is apparently unique to enterobacteria. It has been known for over 20 years that it has many properties which are quite different from those of the other two enterobacterial AHASs isozymes, as well as from those of "typical" AHASs which are single enzymes in a given organism. These include a unique mechanism for regulation of expression and the absence of a preference for forming acetohydroxybutyrate. We have cloned the two subunits, ilvB and ilvN, of this Escherichia coli isoenzyme and examined the enzymatic properties of the purified holoenzyme and the enzyme reconstituted from purified subunits. Unlike other AHASs, AHAS I demonstrates cooperative feedback inhibition by valine, and the kinetics fit closely to an exclusive binding model. The formation of acetolactate by AHAS I is readily reversible and acetolactate can act as substrate for alternative AHAS I-catalyzed reactions.
authors.affiliation: Department of Life Sciences, Ben-Gurion University of the Negev, POB 657, Beer-Sheva 84105, Israel.
authors.forename: Valerie
authors.initials: V
authors.lastname: Vinogradov
authors.forename: Maria
authors.initials: M
authors.lastname: Vyazmensky
authors.forename: Stanislav
authors.initials: S
authors.lastname: Engel
authors.forename: Inna
authors.initials: I
authors.lastname: Belenky
authors.forename: Alexander
authors.initials: A
authors.lastname: Kaplun
authors.forename: Olga
authors.initials: O
authors.lastname: Kryukov
authors.forename: Ze'ev
authors.initials: Z
authors.lastname: Barak
authors.forename: David M
authors.initials: DM
authors.lastname: Chipman
mesh_terms: D000095:Acetolactate Synthase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000096:Acetone / Q000031:analogs & derivatives / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000378:metabolism*; D025461:Feedback, Physiological; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D007536:Isomerism; D007700:Kinetics; D014633:Valine / Q000494:pharmacology
chemical_list: D029968:Escherichia coli Proteins; D007527:Isoenzymes; D000096:Acetone; C035293:1-hydroxy-1-phenyl-2-propanone; D000095:Acetolactate Synthase; C510092:IlvB protein, E coli; C510093:IlvN protein, E coli; D014633:Valine