title: Changes in peripheral leukocytes enzymes activity and plasma metabolite concentrations in growing Holstein calves.
abstract: Plasma metabolite and immunoreactive insulin concentrations and activities of enzymes related to energy metabolism in peripheral leukocytes were measured in growing Holstein calves. A ratio of girth of abdomen divided by girth of thorax (A/T ratio) of calves was significantly elevated after weaning, and the A/T ratio maybe a good indicator to evaluate rumen development. Plasma glucose and free fatty acid concentrations were changed in calves accompanying change in feeding. Activities of lactate dehydrogenase with pyruvate as substrate (LDH-P) and hexokinase (HK) in cytosolic fractions of peripheral leukocytes decreased significantly after weaning the calves reflecting the change of energy source from milk replacer with high percentages of fat and glucose and lactose as absorbable carbohydrate to pelleted feed containing starch as less absorbable carbohydrate and roughage. Some peripheral leukocyte enzymes such as LDH and HK may be good indicators to evaluate changes in energy metabolism of growing calves.
authors.affiliation: Department of Veterinary Science, Nippon Veterinary and Animal Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan. tarai@nvau.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Arai
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Urabe
authors.forename: A
authors.initials: A
authors.lastname: Kusaba
authors.forename: H
authors.initials: H
authors.lastname: Tazaki
authors.forename: T
authors.initials: T
authors.lastname: Ozawa
authors.forename: N
authors.initials: N
authors.lastname: Kimura
authors.forename: K K
authors.initials: KK
authors.lastname: Jung
authors.forename: K
authors.initials: K
authors.lastname: Waragaya
authors.forename: T
authors.initials: T
authors.lastname: Yuyama
authors.forename: Y
authors.initials: Y
authors.lastname: Haseba
authors.forename: S
authors.initials: S
authors.lastname: Imai
mesh_terms: D000005:Abdomen / Q000033:anatomy & histology; D000821:Animal Feed; D000818:Animals; D001699:Biometry; D002417:Cattle / Q000097:blood / Q000254:growth & development* / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000378:metabolism; D004734:Energy Metabolism; D006593:Hexokinase / Q000378:metabolism; D007328:Insulin / Q000032:analysis*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D010949:Plasma / Q000378:metabolism; D013909:Thorax / Q000033:anatomy & histology; D014886:Weaning
chemical_list: D004040:Dietary Carbohydrates; D007328:Insulin; D007770:L-Lactate Dehydrogenase; D006593:Hexokinase