Cloning of cDNAs with PDCD2(C) domain and their expressions during apoptosis of HEK293T cells.
There are only two isoforms of PDCD2 and MGC13096 containing PDCD2(C) domain in human genome. To study the role of PDCD2_C domain in apoptosis, the cDNAs of two isoforms of PDCD2 and MGC13096 were cloned. The RT-PCR products (AY948416, AY948417) of PDCD2 from RNA of human embryonic kidney 293T (HEK293T) and gastric cancer AGS cell line lost common 99 bp when compared with the sequences of NCBI database (NM_002598, NM_144781). The data of expression of PDCD2 and MGC13096 genes in HEK293T cells which induced to undergo apoptosis by various treatments suggested that there was no significant over-regulation of MGC13096 gene and the over-expression of PDCD2 gene did not occur universally. We searched GEO (Gene Expression Omnibus) about PDCD2 and MGC13096. PDCD2 (NM_002598) was over expressed when endothelial cells treated with leukotriene D4 or natural killer cells were activated by IL-2. Perhaps PDCD2_C domain is not universally associated with apoptosis, the function of PDCD2_C domain needs to be studied further.
Chen
Qiu
Q
College of Life Science, Zhejiang University, Hangzhou, P.R. China. happyhappyqiu@hotmail.com
Qian
Kaixian
K
Yan
Chengqi
C
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins; D002460:Cell Line; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA / Q000032:analysis; D018076:DNA, Complementary / Q000235:genetics*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; C094601:PDCD2 protein, human; C000620221:PDCD2L protein, human; D020033:Protein Isoforms; D012333:RNA, Messenger; D004247:DNA