title: Bleomycin has antiviral properties against drug-resistant HIV strains and sensitises virus to currently used antiviral agents.
abstract: In this study we performed phenotypic assays to assess involvement of the cancer chemotherapeutic agent bleomycin (BLM) in replication inhibition of mutant HIV-1 viral strains. Three clinically relevant mutant HIV variants, including one containing the Q151M mutation conferring multinucleoside resistance, were equally as sensitive to BLM as the wild-type HXB2 strain. Long-term incubation of BLM with a wild-type HIV(Ba-L) strain did not alter the sensitivity of the strain to BLM (IC(50) of BLM 0.64 microM at the beginning of incubation to 0.58 microM). At the same point in time, resistance to lamivudine (3TC) and zidovudine (AZT) was noted. Interestingly, the BLM-treated virus showed hypersensitivity to both AZT and 3TC. Our results suggest a contribution of BLM in viral load reduction in patients receiving both anticancer and antiviral agents and harbouring both wild-type and resistant HIV strains.
authors.affiliation: Eijkman-Winkler Center for Microbiology, Infectious Diseases and Inflammation, University Medical Center Utrecht, The Netherlands.
authors.forename: Niki A
authors.initials: NA
authors.lastname: Georgiou
authors.forename: Tjomme
authors.initials: T
authors.lastname: van der Bruggen
authors.forename: Derek M C
authors.initials: DM
authors.lastname: Healy
authors.forename: Carla
authors.initials: C
authors.lastname: van Tienen
authors.forename: Prim
authors.initials: P
authors.lastname: de Bie
authors.forename: Maroeska
authors.initials: M
authors.lastname: Oudshoorn
authors.forename: Joannes J M
authors.initials: JJ
authors.lastname: Marx
authors.forename: B Sweder
authors.initials: BS
authors.lastname: van Asbeck
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology; D002478:Cells, Cultured; D024882:Drug Resistance, Viral; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D019259:Lamivudine / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000821:virology; D008826:Microbial Sensitivity Tests; D009154:Mutation; D015215:Zidovudine / Q000494:pharmacology
chemical_list: D019380:Anti-HIV Agents; D001761:Bleomycin; D019259:Lamivudine; D015215:Zidovudine