Differential gene expression and characterization of tissue-specific cDNA clones in oil palm using mRNA differential display.
The mRNA differential display method was utilized to study the differential expression and regulation of genes in two species of oil palm, the commercially grown variety Elaeis guineensis, var. tenera and the South American species, Elaeis oleifera. We demonstrated the differential expression of genes in the mesocarp and kernel at the week of active oil synthesis (15 week after anthesis) during fruit development as compare to the roots and leaves and the isolation of tissue-specific and species-specific cDNA clones. A total of eight specific cDNA clones were isolated and their specificities were confirmed by Northern hybridization and classified into three groups. Group one contains four clones (KT3, KT4, KT5 and KT6) that are kernel-specific for E. guineensis, tenera and E. oleifera. The second group represents clone FST1, which is mesocarp and kernel-specific for E. guineensis, tenera and E. oleifera. The third group represents clones MLT1, MLT2 and MLO1 that are mesocarp and leaf-specific. Northern analysis showed that their expressions were developmentally regulated. Nucleotide sequencing and homology search in GenBank data revealed that clones KT3 and KT4 encode for the same maturation protein PM3. While clones MLT1 and MLT2 encode for S-ribonuclease binding protein and fibrillin, respectively. The other clones (KT5, KT6, FST1 and MLO1) did not display any significant homology to any known protein.
San
Cha Thye
CT
Department of Biological Sciences, Faculty of Science and Technology, University College of Science and Technology Malaysia, Mengabang Telipot, 21030, Kuala Terengganu, Terengganu, Malaysia.
Shah
Farida Habib
FH
D000595:Amino Acid Sequence; D028023:Arecaceae / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D015152:Blotting, Northern; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D000073878:Palm Oil; D018515:Plant Leaves / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D018076:DNA, Complementary; D010938:Plant Oils; D000073878:Palm Oil