title: [Effect of olfactory ensheathing cells transplantation on protecting spinal cord and neurons after peripheral nerve injury].
abstract: OBJECTIVE
  To study the effect of olfactory ensheathing cells (OECs) transplantation on protecting spinal cord and neurons after peripheral nerve injury.
  
  
  METHODS
  Fifty-five SD rats were randomly divided into blank group (n = 5), experimental group (n = 25) and control group (n = 25). The right sciatic nerves of all the rats were transected. The proximal end was embedded in muscle and treated with OECs (experimental group) and DMEM (control group). No treatment was given to the blank group. The rats were sacrificed 1, 2, 3, 7, and 14 days after the transplantation, the related neurons were observed with histological and TUNEL methods.
  
  
  RESULTS
  After sciatic nerves were transected, death of neurons occurred in spinal cord and ganglion. One, 2, 3 days after treatment, the neuron survival rate in experimental group was 98.4% +/- 6.5%, 97.6% +/- 6.5%, 95.2% +/- 6.7% respectively. The neuron survival rate in control group was 97.8% +/- 6.7%, 97.4% +/- 6.4%, 94.3% +/- 6.8% 1, 2, and 3 days after treatment respectively. There was no significant difference between experimental group and control group. Seven and 14 days after treatment, the neuron survival rate in experimental group was 92.4% +/- 8.9%, 87.7% +/- 9.4% respectively. The neuron survival rate in control group was 87.4% +/- 8.6%, 83.4% +/- 8.5% 7 and 14 days after treatment respectively. There was significant difference between experimental group and control group. On 1st and 2nd day, no apoptosis was seen in spinal cord anterior horn of the rats in both experimental group and control group. On 3rd, 7th, and 14th day, the apoptosis index of spinal cord anterior horn motoneuron in experimental rats were lower (1.2+/-0.8, 1.4 +/- 0. 6, 4.1 +/- 1. 3) than that in the control group (2.1 +/- 1.1, 3.1 +/- 1.1, 6.1 +/- 1.8) (P < 0.05). One, 2, and 3 days after the operation, no ganglion neurons apoptosis was observed in all rats. On 7th day the apoptosis index of ganglion neurons in experimental group(2.10 +/- 0.32) were lower than that in control group (4.40+/-0.56) (P<0.05). On 14th day there was no significant difference in the apoptosis index of ganglion neurons between experimental group (4.30+/-1.80) and control group (6.70 +/- 2.50) (P<0.05).
  
  
  CONCLUSIONS
  Apoptosis of neurons occur after peripheral nerve injury in spinal cord and ganglion. OECs transplantation is effective in preventing apoptosis.
authors.affiliation: Department of Orthopedics, the Second Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710004, P. R. China. wcs420@163.com
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Wang
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Shi
authors.forename: Kunzheng
authors.initials: K
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D017690:Cell Transplantation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D005724:Ganglia / Q000473:pathology; D008297:Male; D009474:Neurons / Q000473:pathology*; D009830:Olfactory Bulb / Q000166:cytology*; D059348:Peripheral Nerve Injuries; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries / Q000601:surgery; D013116:Spinal Cord / Q000473:pathology