title: Protective effects of flavonols and their glycosides against free radical-induced oxidative hemolysis of red blood cells.
abstract: The in vitro oxidative hemolysis of human red blood cells (RBCs) was used as a model to study the free radical-induced damage of biological membranes and the protective effect of flavonols and their glycosides (FOHs), i.e., myricetin (MY), quercetin (Q), morin (MO), kaempferol (K), rutin (R), quercetin galactopyranoside (QG), quercetin rhamnopyranoside (QR), and kaempferol glucopyranoside (KG). The hemolysis of RBCs was induced by a water-soluble free radical initiator 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AAPH). It was found that addition of AAPH at 37 degrees C to the suspension of RBCs caused fast hemolysis after a short period of inhibition period, and addition of FOHs significantly suppressed the hemolysis. The FOHs (MY, Q, R, QG and QR) which bears an ortho-dihydroxyl functionality showed much more effective anti-hemolysis activity than that of the other FOHs (MO, K and KG) bearing no such functionality.
authors.affiliation: National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Dai
authors.forename: Qing
authors.initials: Q
authors.lastname: Miao
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Liu
mesh_terms: D000578:Amidines / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D044948:Flavonols / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D024502:alpha-Tocopherol / Q000494:pharmacology
chemical_list: D000578:Amidines; D000975:Antioxidants; D044948:Flavonols; D016166:Free Radical Scavengers; D005609:Free Radicals; D006027:Glycosides; C046728:2,2'-azobis(2-amidinopropane); D024502:alpha-Tocopherol