title: How to build a central synapse: clues from cell culture.
abstract: Central neurons develop and maintain molecularly distinct synaptic specializations for excitatory and inhibitory transmitters, often only microns apart on their dendritic arbor. Progress towards understanding the molecular basis of synaptogenesis has come from several recent studies using a coculture system of non-neuronal cells expressing molecules that generate presynaptic or postsynaptic "hemi-synapses" on contacting neurons. Together with molecular properties of these protein families, such studies have yielded interesting clues to how glutamatergic and GABAergic synapses are assembled. Other clues come from heterochronic cultures, manipulations of activity in subsets of neurons in a network, and of course many in vivo studies. Taking into account these data, we consider here how basic parameters of synapses--competence, placement, composition, size and longevity--might be determined.
authors.affiliation: Brain Research Centre and Department of Psychiatry, University of British Columbia, Vancouver, Canada V6T 2B5. amcraig@interchange.ubc.ca
authors.forename: Ann Marie
authors.initials: AM
authors.lastname: Craig
authors.forename: Ethan R
authors.initials: ER
authors.lastname: Graf
authors.forename: Michael W
authors.initials: MW
authors.lastname: Linhoff
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005971:Glutamates / Q000378:metabolism*; D006801:Humans; D008959:Models, Neurological; D009474:Neurons / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D005971:Glutamates; D018377:Neurotransmitter Agents; D005680:gamma-Aminobutyric Acid