Influence of plant species on population dynamics, genotypic diversity and antibiotic production in the rhizosphere by indigenous Pseudomonas spp.
The population dynamics, genotypic diversity and activity of naturally-occurring 2,4-diacetylphloroglucinol (DAPG)-producing Pseudomonas spp. was investigated for four plant species (wheat, sugar beet, potato, lily) grown in two different soils. All four plant species tested, except lily and in some cases wheat, supported relatively high rhizosphere populations (5 x 10(4) to 1 x 10(6) CFU/g root) of indigenous DAPG-producing Pseudomonas spp. during successive cultivation in both a take-all suppressive and a take-all conducive soil. Although lily supported on average the highest population densities of fluorescent Pseudomonas spp., it was the least supportive of DAPG-producing Pseudomonas spp. of all four plant species. The genotypic diversity of 492 DAPG-producing Pseudomonas isolates, assessed by Denaturing Gradient Gel Electrophoresis (DGGE) analysis of the phlD gene, revealed a total of 7 genotypes. Some of the genotypes were found only in the rhizosphere of a specific plant, whereas the predominant genotypes were found at significantly higher frequencies in the rhizosphere of three plant species (wheat, sugar beet and potato). Statistical analysis of the phlD(+) genotype frequencies showed that the diversity of the phlD(+) isolates from lily was significantly lower than the diversity of phlD(+) isolates found on wheat, sugar beet or potato. Additionally, soil type had a significant effect on both the phlD(+) population density and the phlD(+) genotype frequencies, with the take-all suppressive soil being the most supportive. HPLC analysis further showed that the plant species had a significant effect on DAPG-production by the indigenous phlD(+) population: the wheat and potato rhizospheres supported significantly higher amounts of DAPG produced per cell basis than the rhizospheres of sugar beet and lily. Collectively, the results of this study showed that the host plant species has a significant influence on the dynamics, composition and activity of specific indigenous antagonistic Pseudomonas spp.
Bergsma-Vlami
Maria
M
Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, P.O. Box 8025, 6709, PD Wageningen, The Netherlands.
Prins
Mieke E
ME
Raaijmakers
Jos M
JM
D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D014644:Genetic Variation; D005838:Genotype; D019684:Magnoliopsida / Q000382:microbiology*; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology*; D011157:Population Dynamics; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D012988:Soil Microbiology; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D017931:DNA Primers; C096183:PhlD protein, Pseudomonas; C059817:2,4-diacetylphloroglucinol; D010696:Phloroglucinol