title: Novel polymorphisms at codons 146 and 151 in the prion protein gene of Cyprus goats, and their association with natural scrapie.
abstract: To discern whether an association exists between specific combinations of polymorphisms of the prion protein (PrP) and natural scrapie in Cyprus goats, 250 goats were examined, including 164 histologically positive cases. Previously reported amino acid polymorphisms were detected at codons 154 (R-->H), 168(P-->Q), 220(Q-->H) and 240 (S-->P) and nucleotide alterations at codons 42 (a-->g) and 138 (c-->t). Additionally, novel amino acid polymorphisms were detected at codons 146 (N-->S or D) and 151 (R-->H) and new "silent" mutations were found at codons 179 (V,g-->t), 181 (D,c-->t) and 219 (T,c-->t). The two novel polymorphisms at codon 146 were found only in the healthy control and scrapie-negative goats. By comparison, none of the scrapie-affected goats encoded these polymorphisms.
authors.affiliation: Virology Laboratory, Veterinary Services, 1417 Nicosia, Cyprus. penelope.s@cytanet.com.cy
authors.forename: Penelope
authors.initials: P
authors.lastname: Papasavva-Stylianou
authors.forename: Marina
authors.initials: M
authors.lastname: Kleanthous
authors.forename: Pavlos
authors.initials: P
authors.lastname: Toumazos
authors.forename: Petroula
authors.initials: P
authors.lastname: Mavrikiou
authors.forename: Phedias
authors.initials: P
authors.lastname: Loucaides
mesh_terms: D000818:Animals; D001483:Base Sequence; D003062:Codon / Q000235:genetics*; D003535:Cyprus; D005838:Genotype; D015511:Goat Diseases / Q000235:genetics*; D006041:Goats; D011110:Polymorphism, Genetic / Q000235:genetics*; D011328:Prions / Q000235:genetics*; D012608:Scrapie / Q000235:genetics*
chemical_list: D003062:Codon; D011328:Prions