[Influence of estradiol benzoate on fluid reabsorption capacity of rat efferent ductuli].
OBJECTIVE
To study the influence of estradiol benzoate (E2B) on the fluid reabsorption capacity of rat efferent ductuli.


METHODS
Newborn male Sprague-Dawley rats were injected subcutaneously with E2B (0.2 mg/5 g body weight), and the histological morphology of efferent ductulus, epithelial ultrastructure, and immunoexpression of AQP-1 were investigated on postnatal day 14, 21, 28, 42 and 56, respectively. Vehicle was given to the controls.


RESULTS
After exposure to E2B, the lumina of the efferent ductuli dilated greatly (P < 0.05), and the epithelium height decreased significantly (P < 0.01), microvilli of nonciliated cells short and sparse, endocytic apparatus implicated in fluid reabsorption scarce, and with no AQP-1 expression.


CONCLUSIONS
High dosage of E2 B neonatally administrated to rats damages the fluid reabsorption capacity of efferent ductuli.
Zhang
Quan
Q
Department of Urology, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, China. zhangquan1291@sina.com.cn
Zhou
Xin-Hua
XH
Zhang
Xiao-Bin
XB
Zhou
Qing-Hui
QH
Wang
De-Zhi
DZ
Chen
Xi-Chang
XC
D000042:Absorption / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D051398:Aquaporin 1 / Q000096:biosynthesis; D001826:Body Fluids / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004958:Estradiol / Q000031:analogs & derivatives* / Q000633:toxicity; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
C496022:Aqp1 protein, rat; D051398:Aquaporin 1; C074283:estradiol 3-benzoate; D004958:Estradiol