title: Acute rejection is associated with antibodies to non-Gal antigens in baboons using Gal-knockout pig kidneys.
abstract: We transplanted kidneys from alpha1,3-galactosyltransferase knockout (GalT-KO) pigs into six baboons using two different immunosuppressive regimens, but most of the baboons died from severe acute humoral xenograft rejection. Circulating induced antibodies to non-Gal antigens were markedly elevated at rejection, which mediated strong complement-dependent cytotoxicity against GalT-KO porcine target cells. These data suggest that antibodies to non-Gal antigens will present an additional barrier to transplantation of organs from GalT-KO pigs to humans.
authors.affiliation: [1] Department of Surgery, University of Western Ontario, London, Ontario N6A 5C1, Canada.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Qian
authors.forename: Thomas
authors.initials: T
authors.lastname: Starzl
authors.forename: Hongtao
authors.initials: H
authors.lastname: Sun
authors.forename: Bertha
authors.initials: B
authors.lastname: Garcia
authors.forename: Ximo
authors.initials: X
authors.lastname: Wang
authors.forename: Yishai
authors.initials: Y
authors.lastname: Wise
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiang
authors.forename: Laura
authors.initials: L
authors.lastname: Copeman
authors.forename: Weihua
authors.initials: W
authors.lastname: Liu
authors.forename: Anthony
authors.initials: A
authors.lastname: Jevnikar
authors.forename: William
authors.initials: W
authors.lastname: Wall
authors.forename: David K C
authors.initials: DK
authors.lastname: Cooper
authors.forename: Noriko
authors.initials: N
authors.lastname: Murase
authors.forename: Yifan
authors.initials: Y
authors.lastname: Dai
authors.forename: Wanyu
authors.initials: W
authors.lastname: Wang
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Xiong
authors.forename: David J
authors.initials: DJ
authors.lastname: White
authors.forename: Robert
authors.initials: R
authors.lastname: Zhong
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000906:Antibodies / Q000097:blood; D005700:Galactosyltransferases / Q000235:genetics / Q000276:immunology; D006084:Graft Rejection / Q000276:immunology*; D007108:Immune Tolerance / Q000276:immunology*; D007165:Immunosuppression Therapy / Q000379:methods; D016030:Kidney Transplantation / Q000276:immunology*; D010215:Papio; D034421:Sus scrofa / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology; D014183:Transplantation, Heterologous / Q000276:immunology / Q000379:methods* / Q000401:mortality*
chemical_list: D000906:Antibodies; D005700:Galactosyltransferases