[Mechanism of AcrAB-TolC system mediated multiple antimicrobial resistance in Salmonella typhimurium].
OBJECTIVE
To confirm AcrAB-TolC system mediated the multiple antimicrobial resistance in Salmonella typhimurium.


METHODS
One-step knock-out of chromosomal method was used, to disrupt chromosomal genes, in Salmonella typhimurium wild strain X2. The acrAB gene and tolC gene were knocked out and two mutants of the delta acrABX2 and delta tolCX2 were produced.


RESULTS
Results of E-test to two mutation strains and their parent strain showed that delta acrABX2 and delta tolCX2 were resistant to vancomycin and susceptible to amikacin and cephalothin, which was similar to their parents isolate X2, but (acrABX2 and (tolCX2 were susceptible to other eleven antimicrobial agents, while the parents isolate X2 was resistant to those.


CONCLUSIONS
multiple antimicrobial resistance in Salmonella typhimurium. It suggested that the AcrAB-TolC system mediated the multiple antimicrobial resistance in Salmonella typhimurium.
Guo
Yun-Chang
YC
National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Du
Chun-Ming
CM
Liu
Xiu-Mei
XM
D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D055786:Gene Knockout Techniques; D009154:Mutation; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics*; D020713:Vancomycin Resistance
C493251:AcrAB-TolC protein, Salmonella enterica; D001426:Bacterial Proteins; D002352:Carrier Proteins