title: Triallelic patterns in STR loci used for paternity analysis: evidence for a duplication in chromosome 2 containing the TPOX STR locus.
abstract: We report triallelic patterns in several short tandem repeat (STR) loci revealed by routine paternity testing using the commercial AMPFlSTR Profiler and AMPFlSTR SGMplus kits. One case where the TPOX-locus (2p25.3) produced three peaks from the blood sample of a child was analysed further. Quantitative polymerase chain reaction (QPCR) and STR typing of the DNAs of the family trio revealed a large (>1.59 Mb) duplication flanking the TPOX-locus in chromosome 2 in both the mother and child. The implications of such genetic anomalies for paternity testing are discussed.
authors.affiliation: Paternity Testing Laboratory, Department of Molecular Medicine, National Public Health Institute, Mannerheimintie 166, 00300 Helsinki, Finland.
authors.forename: Matti
authors.initials: M
authors.lastname: Lukka
authors.forename: Gunnar
authors.initials: G
authors.lastname: Tasa
authors.forename: Pekka
authors.initials: P
authors.lastname: Ellonen
authors.forename: Kirsi
authors.initials: K
authors.lastname: Moilanen
authors.forename: Vitali
authors.initials: V
authors.lastname: Vassiljev
authors.forename: Ismo
authors.initials: I
authors.lastname: Ulmanen
mesh_terms: D002648:Child; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D019075:Electrophoresis, Capillary; D005260:Female; D053803:Forensic Genetics; D020440:Gene Duplication; D005819:Genetic Markers; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
chemical_list: D005819:Genetic Markers