HPTLC-densitometric method of determination of oleanolic acid in the Lamii albi flos.
HPTLC combined with densitometric method was used to determine the content of oleanolic acid in acetone extract from the Lamii albi flos L. The plant extract was separated on Si 60 HPTLC plates and determined with the use of two densitometric methods: measurement of fluorescence and absorbance. The content of oleanolic acid was statistically calculated. In a measurement of absorbance in 1 g of the dry Lamii albi flos L. 1458 microg of oleanolic acid was determined (0.147%). In a fluorescence method in 1 g of the plant material 1516 microg of oleanolic acid was determined (0.149%).
Wójciak-Kosior
Magdalena
M
Laboratory of Planar Chromatography, Department of Chemistry, Medical Academy, Lublin, Poland. kosiorma@wp.pl
Krzaczek
Tadeusz
T
Matysik
Grazyna
G
Skalska
Agnieszka
A
D000096:Acetone; D002851:Chromatography, High Pressure Liquid; D003720:Densitometry; D009828:Oleanolic Acid / Q000032:analysis* / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D010936:Plant Extracts; D000096:Acetone; D009828:Oleanolic Acid