Hydrolysis of plasmid DNA and RNA by amino alkyl naphthalimide as metal-free artificial nuclease.
A strategy of dimethylamino alkyldiimide conjugated with an intercalator of naphthalimide for hydrolysis of DNA was suggested and evaluated. 4 can hydrolyze 4 kb plasmid DNA into 2 kb fragments with GC and GG selectivity, which represents a novel example of sequence- or site-selective metal-free DNA artificial nuclease. Results also show it could hydrolyze RNA efficiently.
Yang
Qing
Q
Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116024, China. qingyang@dlut.edu.cn
Xu
Jianqiang
J
Sun
Yuanshe
Y
Li
Zhigang
Z
Li
Yonggang
Y
Qian
Xuhong
X
D004247:DNA / Q000737:chemistry*; D003851:Deoxyribonucleases / Q000138:chemical synthesis / Q000737:chemistry*; D015195:Drug Design; D006860:Hydrogen Bonding; D006868:Hydrolysis; D007094:Imides / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D009281:Naphthalenes / Q000138:chemical synthesis / Q000737:chemistry*; D010957:Plasmids; D012313:RNA / Q000737:chemistry*; D012260:Ribonucleases / Q000138:chemical synthesis / Q000737:chemistry*
D007094:Imides; D009281:Naphthalenes; D012313:RNA; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases