[Reconstruction of dentin-pulp complex structure by tissue engineering technology].
OBJECTIVE
To investigate the possibility of reconstruction of dentin-pulp complex by tissue engineering technology.


METHODS
Rat dental pulp stem cells were seeded into HA-TCP scaffold and incubated for 20 hours in vitro. Then the cell-scaffold complex was implanted subcutaneously into the dorsal side of nude mice. 8 weeks postimplantation, the samples were extracted for histological and immunohistochemical examinations.


RESULTS
Three strata of tissue were observed in the hole of HA-TCP scaffold. They were dentin-like tissue, predentin-like tissue and pulp-like tissue respectively from the inner surface of the pore to the center. Dentin tubules were obvious in predentin-like and dentin-like tissue lining from the pulp-like tissue through predentin-like tissue and dentin-like tissue. Cells localized along the edge of pulp-like tissue were dense and polarized, resembling odontoblasts. Immunohistochemical study demonstrated DSP and DMP1 expression in these odontoblast-like cells and in the area of predentin-like tissue.


CONCLUSIONS
Tissue-engineered rat dentin-pulp complex was reconstructed by seeding HA-TCP scaffold with rat dental pulp stem cells.
Guo
Hong-yan
HY
Department of Stomatology, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
Wu
Bu-ling
BL
Guo
Xi-min
XM
Yang
Cheng
C
Xu
Peng
P
Wang
Chang-yong
CY
D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology; D003804:Dentin / Q000166:cytology; D005260:Female; D006882:Hydroxyapatites / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D051381:Rats; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
D002130:Calcium Phosphates; D006882:Hydroxyapatites