title: Salt is necessary for nucleosomal DNA fragmentation induced by caspase.
abstract: For nucleosomal DNA fragmentation, one of the hallmarks of apoptosis, activated caspase, an apoptosis specific cysteine protease, is required to cleave ICAD/DFF45 that releases its complexed DNase, CAD/DFF40. The protein complex is located predominantly in the nuclei. Inconsistently, caspase alone cannot induce DNA fragmentation in the isolated nuclei without the addition of a cell extract or purified CAD/DFF40. In this study, however, it is demonstrated that under selected conditions with 50-75 mM: KCl or NaCl, caspase-3 and-7 can induce DNA fragmentation without the additional factor(s).
authors.affiliation: Research Center for Proteineous Materials (RCPM) and Department of Bio-Materials Engineering, Chosun University, 375 Seosuk-Dong, Dong-Ku, Gwangju 501-759, Republic of Korea.
authors.forename: Bo-Eun
authors.initials: BE
authors.lastname: Kim
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Lee
authors.forename: Changhyun
authors.initials: C
authors.lastname: Seong
authors.forename: Song Yub
authors.initials: SY
authors.lastname: Shin
authors.forename: Jung Sup
authors.initials: JS
authors.lastname: Lee
authors.forename: Byung Yun
authors.initials: BY
authors.lastname: Cho
authors.forename: Il-Seon
authors.initials: IS
authors.lastname: Park
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins; D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D053148:Caspase 3; D061945:Caspase Inhibitors; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism; D002850:Chromatography, Gel; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects*; D003851:Deoxyribonucleases / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D009154:Mutation / Q000235:genetics; D009707:Nucleosomes / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D000075223:Poly-ADP-Ribose Binding Proteins; D011189:Potassium Chloride / Q000494:pharmacology; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012492:Salts / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D051017:Apoptosis Regulatory Proteins; D061945:Caspase Inhibitors; D002843:Chromatin; D015853:Cysteine Proteinase Inhibitors; D009707:Nucleosomes; D009842:Oligopeptides; D000075223:Poly-ADP-Ribose Binding Proteins; D011506:Proteins; D012492:Salts; C108637:aspartyl-glutamyl-valyl-aspartal; C109664:caspase-activated DNase inhibitor; C118632:tyrosyl-valyl-alanyl-aspartal; D001312:Aurintricarboxylic Acid; D012965:Sodium Chloride; D011189:Potassium Chloride; D003851:Deoxyribonucleases; C491882:Dffb protein, rat; C505407:CASP3 protein, human; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases