Evaluation of new broth media for microdilution antibiotic susceptibility testing of Lactobacilli, Pediococci, Lactococci, and Bifidobacteria.
Nine pure or mixed broth media were evaluated for their suitabilities to determine MICs in a microdilution test of 19 antibacterial agents for lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Lactococcus, and Bifidobacterium. A mixed formulation of Iso-Sensitest broth (90%) and deMan-Rogosa-Sharpe broth (10%) with or without supplementation with L-cysteine, referred to as the LAB susceptibility test medium, provided the most optimal medium basis in terms of growth support of nonenterococcal LAB and correct indication of MICs of international control strains.
Klare
Ingo
I
Robert Koch Institute, Wernigerode Branch, Burgstrasse 37, D-38855 Wernigerode, Germany. i.klare@rki.de
Konstabel
Carola
C
Müller-Bertling
Sibylle
S
Reissbrodt
Rolf
R
Huys
Geert
G
Vancanneyt
Marc
M
Swings
Jean
J
Goossens
Herman
H
Witte
Wolfgang
W
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001644:Bifidobacterium / Q000187:drug effects*; D003470:Culture Media; D016983:Enterococcus / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D007778:Lactobacillus / Q000187:drug effects*; D017045:Lactococcus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010376:Pediococcus / Q000187:drug effects*; D013210:Staphylococcus / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D003470:Culture Media