Optimal genotype determination in highly multiplexed SNP data.
High-throughput genotyping technologies that enable large association studies are already available. Tools for genotype determination starting from raw signal intensities need to be automated, robust, and flexible to provide optimal genotype determination given the specific requirements of a study. The key metrics describing the performance of a custom genotyping study are assay conversion, call rate, and genotype accuracy. These three metrics can be traded off against each other. Using the highly multiplexed Molecular Inversion Probe technology as an example, we describe a methodology for identifying the optimal trade-off. The methodology comprises: a robust clustering algorithm and assessment of a large number of data filter sets. The clustering algorithm allows for automatic genotype determination. Many different sets of filters are then applied to the clustered data, and performance metrics resulting from each filter set are calculated. These performance metrics relate to the power of a study and provide a framework to choose the most suitable filter set to the particular study.
Moorhead
Martin
M
ParAllele BioScience, South San Francisco, CA, USA.
Hardenbol
Paul
P
Siddiqui
Farooq
F
Falkowski
Matthew
M
Bruckner
Carsten
C
Ireland
James
J
Jones
Hywel B
HB
Jain
Maneesh
M
Willis
Thomas D
TD
Faham
Malek
M
D000465:Algorithms; D016000:Cluster Analysis; D003198:Computer Simulation; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D015336:Molecular Probe Techniques; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015203:Reproducibility of Results; D012107:Research Design
D005819:Genetic Markers