An antifungal peptide from the coconut.
A chromatographic procedure consisting of ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, and gel filtration by fast performance liquid chromatography on Supedex 75 was utilized to isolate a 10 kDa antifungal peptide from coconut flesh. The peptide was unadsorbed on DEAE-cellulose, but adsorbed on Affi-gel blue gel and CM-cellulose. It displayed antifungal activity against Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola. The IC50 values of its inhibitory activities on mycelial growth in M. arachidicola and HIV-1 reverse transcriptase activity were respectively 1.2 and 52.5 microM.
Wang
H X
HX
Department of Microbiology, China Agricultural University and State Key Laboratory of Agrobiotechnology, Beijing, China.
Ng
T B
TB
D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003059:Cocos / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D008826:Microbial Sensitivity Tests; D009754:Nuts / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D000935:Antifungal Agents; D010455:Peptides; D010940:Plant Proteins; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase