[Study on effect of ginsenoside Rg1 in promoting myocardiac vascular endothelial cell regeneration through induction on bone marrow stem cell's migration and differentiation in rabbits of myocardial infarction].
OBJECTIVE
To observe whether ginsenoside Rg1 could reduce the infarcted area and improve the heart function by path of promoting bone marrow stem cells differentiated to vascular endothelial cells (VECs).


METHODS
Bone marrow was drawn from rabbit's ilium and labelled with red fluorochrome DiI, then it was transferred again into the rabbit's body. The rabbits was then made into myocardiac infarction model. The model rabbits were divided into the control group and the ginsenoside Rgl treated group (treated group). The infracted area at two weeks, and the left ventricular function at one and two weeks after infarction were determined respectively. The DiI positive cell rate of myelogenetic cells in ischmia area and CD31 positive cell rate of VECs were determined by confocal microscopy. Myocardial interstitial granulocyte colony-stimulating factor(GCSF) levels during ischemia and reperfusion period were determined also.


RESULTS
DiI positive rate of CD31 staining positive cells in the treated group was obviously increased, and the concentration of G-CSF in myocardium interstitial obviously increased, accompanied with obviously improving of heart function and obviously reducing of infarcted area.


CONCLUSIONS
Ginsenoside Rgl could stimulate the G-CSF secretion in local myocardiac tissues, thus to induce bone marrow mononuclear cells migrate to myocadial tissue and further differentiate to VECs. The regeneration of endothelium cells show certain direct action in promoting capillary regeneration of infarcted myocardium tissue and maintaining the blood supply.
Wang
Ning-yuan
NY
Department of Cardiology Regeneration Medicine, Gifu University School of Medicine, Gifu, Japan. niyuanwang@hotmail.com
Lu
Chuan-jiang
CJ
Chen
Xue-hai
XH
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D003326:Coronary Circulation / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology*; D036145:Ginsenosides / Q000494:pharmacology*; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis; D008297:Male; D039902:Multipotent Stem Cells / Q000166:cytology*; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology*; D011817:Rabbits
D036145:Ginsenosides; D016179:Granulocyte Colony-Stimulating Factor; C035054:ginsenoside Rg1