Octylcaffeate induced apoptosis in human leukemia U937 cells.
We found that octylcaffeate, a semisynthetic caffeic acid derivative, strongly inhibited the growth of human histiolytic lymphoma U937 cells in a dose- and time-dependent manner via apoptosis. Octylcaffeate induced the fragmentation of DNA into multiples of 180 bp (an apoptotic DNA ladder) and condensation of chromatin, and increased the percentage of hypodiploid cells detected with a flow cytometer. DNA fragmentation induced by octylcaffeate was inhibited by pretreatment with Z-DEVD-FMK and Z-Asp-CH(2)D-CB, an inhibitor of caspase, clearly showing that the mode of cell death is apoptotic. These findings suggest that the cytotoxicity of octylcaffeate involves the induction of apoptosis.
Ujibe
Mayuko
M
Department of Pharmacology and Toxicology, Cancer Research Institute, Tohoku Pharmaceutical University, Sendai 981-8558, Japan.
Kanno
Syu-ichi
S
Osanai
Yu
Y
Koiwai
Kimiko
K
Ohtake
Takaharu
T
Kimura
Katsuhiko
K
Uwai
Kohji
K
Takeshita
Mitsuhiro
M
Ishikawa
Masaaki
M
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002109:Caffeic Acids / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D007945:Leukemia, Lymphoid / Q000188:drug therapy*; D013997:Time Factors; D020298:U937 Cells / Q000187:drug effects
D000970:Antineoplastic Agents; D002109:Caffeic Acids; D004791:Enzyme Inhibitors; C474929:octyl caffeate; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases