Determination of 3-chloropropane-1,2-diol in liquid hydrolyzed vegetable proteins and soy sauce by solid-phase microextraction and gas chromatography/mass spectrometry.
Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography/mass spectrometry (GC/MS) method was developed to determine 3-chloropropane-1,2-diol (3-MCPD) in hydrolyzed vegetable protein and Chinese soy sauce. The 3-MCPD was firstly derivativized with phenylboronic acid in aqueous solution at 90 degrees C for 10 min, then extracted by HS-SPME and finally detected with GC/MS, parameters related to both the derivative reaction and the HS-SPME process were optimized. The proposed method has a linear range of 0.0194-394 microg g(-1), a detection limit of 3.87 ng g(-1) (S/N = 3), and a precision of RSD = 7.5% (n = 5). Seventeen real samples, including four HVPs and thirteen soy sauce samples, were analyzed to examine the feasibility of the proposed procedure; with a concentration of 3-MCPD and acceptable recoveries at 0.71 microg g(-1) spiked levels were obtained. Being simpler, faster and more environmentally benign than the existing methods, this method is accurate and suitable for routine analysis.
Huang
Minjia
M
Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
Jiang
Guibin
G
He
Bin
B
Liu
Jingfu
J
Zhou
Qingxiang
Q
Fu
Wusheng
W
Wu
Yongning
Y
D001897:Boronic Acids / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D007093:Imidazoles / Q000737:chemistry; D014674:Plant Proteins, Dietary / Q000032:analysis*; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000737:chemistry; D045730:Soy Foods / Q000032:analysis*; D013696:Temperature; D000517:alpha-Chlorohydrin / Q000032:analysis* / Q000737:chemistry; D005466:Fluorocarbons
D001897:Boronic Acids; D007093:Imidazoles; D014674:Plant Proteins, Dietary; D012965:Sodium Chloride; D000517:alpha-Chlorohydrin; C046634:1-(heptafluorobutyryl)imidazole; D005466:Fluorocarbons; C010686:benzeneboronic acid