title: Cytomegalovirus-associated periodontitis and Guillain-Barré syndrome.
abstract: BACKGROUND
  Guillain-Barré syndrome (GBS), an autoimmune disorder of the peripheral nervous system, is characterized by rapidly ascending neural paralysis, hyporeflexia, and areflexia. The polyneuropathy of the GBS affects one to four humans per 100,000 of the population annually throughout the world (adults and children). The pathogenesis of GBS remains unclear. However, there are increasing indications that the disease is triggered by a preceding well-established febrile infection by cytomegalovirus (CMV). The present report describes active CMV within the periodontium of a 37-year-old patient affected by GBS.
  
  
  METHODS
  Real-time reverse transcriptase-polymerase chain reaction (real-time RT-PCR) was performed to detect CMV, Epstein-Barr virus-1 (EBV-1), herpes simplex 1 (HSV-1) and 2 (HSV-2) virus, and enteroviruses (polio-, coxsackie-, echo-, and enteroviruses 68 and 71) from periodontal sites demonstrating advanced attachment loss. Healthy sites and sites with inflamed gingival tissue were not included in the study. Anaerobic bacterial culture determined the occurrence of potential major periodontal pathogens.
  
  
  RESULTS
  Real-time RT-PCR and microbiologic analysis revealed the presence of a dual infection of CMV and specific bacterial plaque. CMV, Porphyromonas gingivalis, Tannerella forsythensis, and Campylobacter species were associated with periodontitis active sites, loss of attachment, and gingival bleeding. Furthermore, periodontal sites infected by active CMV had no visible radiographic crestal lamina dura.
  
  
  CONCLUSIONS
  The periodontium may serve as a reservoir for CMV and a source of viral replication. However, further research is needed to test whether viral replication in the periodontium precedes the GBS symptoms.
authors.affiliation: Department of Periodontology, Advanced Periodontology, School of Dentistry, University of Southern California, Los Angeles, CA 90089-0641, USA. giorgiotabanella@tiscalinet.it
authors.forename: Giorgio
authors.initials: G
authors.lastname: Tabanella
authors.forename: Hessam
authors.initials: H
authors.lastname: Nowzari
mesh_terms: D000328:Adult; D001327:Autoimmune Diseases / Q000150:complications; D016866:Bacteroidaceae Infections / Q000175:diagnosis; D001442:Bacteroides Infections / Q000175:diagnosis; D002169:Campylobacter Infections / Q000175:diagnosis; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D003773:Dental Plaque / Q000382:microbiology; D005884:Gingival Hemorrhage / Q000382:microbiology; D020275:Guillain-Barre Syndrome / Q000150:complications*; D006801:Humans; D017622:Periodontal Attachment Loss / Q000821:virology; D010514:Periodontal Pocket / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology / Q000821:virology*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification