[Protective effect of zuogui pill on ovarian autoimmune injury].
OBJECTIVE
To probe the effect of Zuogui pill (ZGP), a Chinese compound recipe for tonifying Shen, on ovarian function in mice with premature ovarian failure (POF).


METHODS
BALB/C female mice model of POF was established by multiple sites subcutaneous injection of ovarian antigen elicited with ovarian tissue of SD female rats, and treated with ZGP at different time points in the modeling, with prednisone as positive control. The levels of follicle-stimulating hormone (FSH) and estradiol (E2) in peripheral blood were measured with radioimmunoassay, and ovarian antibody (AoAb) was determined by enzyme linked immunosorbent assay. The mRNA expression of ovarian growth and differentiation factor-9 was detected with in situ hybridization.


RESULTS
POF model mice manifested such abnormalities as increased FSH, decreased E2, and positive AoAb in peripheral blood, with lymphocytes infiltration in ovarian mesanchyma, reduction of GDF-9 mRNA positive oocytes, and decrease of growing and mature follicles. ZGP could reduce the increase of FSH, increase the level of E2, inhibit the production of AoAb, raise the GDF-9 mRNA positive cells of oocytes, increase the number of growing and mature follicles. The clinical efficacy was more significant in early stage than in advanced stage.


CONCLUSIONS
ZGP can improve immune inflammatory injury of ovary, and shows therapeutic effect on POF.
Zhu
Ling
L
Department of TCM, Southern Medical University, Guangzhou. zhuling20012001@yahoo.com
Luo
Song-ping
SP
Xu
Li-mian
LM
D000818:Animals; D001327:Autoimmune Diseases / Q000209:etiology / Q000517:prevention & control*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D006080:Ovarian Follicle / Q000473:pathology; D010053:Ovary / Q000187:drug effects* / Q000276:immunology / Q000503:physiopathology; D008517:Phytotherapy; D016649:Primary Ovarian Insufficiency / Q000209:etiology / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016710:Yin Deficiency / Q000188:drug therapy
D004365:Drugs, Chinese Herbal