title: Polymorphisms of glutathione S-transferase M1, T1, and P1 in patients with HBV-related liver cirrhosis, chronic hepatitis, and normal carriers.
abstract: OBJECTIVE
  Persistent hepatitis B virus (HBV) infection often leads to the development of chronic hepatitis and cirrhosis. The role of host genetic factors in chronic HBV infection is not fully understood. We studied the influence of glutathione S-transferase (GST) M1, T1, and P1 polymorphisms in patients with different stages of HBV infections.
  
  
  METHODS
  The sample population included 41 HBV normal carriers, 37 patients with chronic hepatitis, and 38 patients with cirrhosis (infected with HBV) compared to a control group (n = 59). PCR-based procedures were performed in the studied populations to confirm the genotypes of GSTT1, M1, and P1. Odds ratio analysis tests were used for statistical evaluation.
  
  
  RESULTS
  We found that the frequency of GSTP1-Val (105)/Val (105) genotype was significantly higher in patients with liver cirrhosis (27%) than HBV normal carriers (2.4%; OR 14.8, 95% CI 1.8-122.5) and the frequency GSTP1-Val (105)/Ile (105) genotype was significantly higher in patients with liver cirrhosis (59.5%) than HBV normal carriers (19.5%; OR 6.1, 95% CI 2.1-16.7). The genotype GSTP1-Val (105)/Val (105) was more frequent in patients with chronic hepatitis (19.4%) than HBV normal carriers (2.4%; OR 9.65, 95% CI 1.1-82.8). Patients with cirrhosis also had a higher frequency of the GSTM1 null genotype (71.1%) than HBV normal carriers (27.5%; OR 6.5, 95% CI 2.4-17.4) and the GSTM1 null genotype was more frequent in patients with chronic hepatitis (64.9%) than HBV normal carriers (27.5%OR 4.9, 95% CI 1.8-12.8). The frequency of GSTT1 genotype was similar in all groups.
  
  
  CONCLUSIONS
  These results suggest that in HBV infection, inheritance of the null GSTM1 and GSTP1-Val (105) polymorphisms involves a host genetic factor that is relevant to disease progression.
authors.affiliation: Department of Biochemistry and Genetics, Shahid Beheshti University of Medical Sciences, Evin, PO Box 19395-4719, Tehran, Iran. arianshs@scientist.com
authors.forename: Shahrokh
authors.initials: S
authors.lastname: Mohammadzadeh Ghobadloo
authors.forename: Bahram
authors.initials: B
authors.lastname: Yaghmaei
authors.forename: Abdolamir
authors.initials: A
authors.lastname: Allameh
authors.forename: Parkhideh
authors.initials: P
authors.lastname: Hassani
authors.forename: Babak
authors.initials: B
authors.lastname: Noorinayer
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Zali
mesh_terms: D000328:Adult; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006509:Hepatitis B / Q000201:enzymology* / Q000235:genetics; D006521:Hepatitis, Chronic / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008103:Liver Cirrhosis / Q000201:enzymology* / Q000235:genetics; D008297:Male; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012044:Regression Analysis
chemical_list: D007527:Isoenzymes; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase