title: Matrine improves 2,4,6-trinitrobenzene sulfonic acid-induced colitis in mice.
abstract: Matrine is an alkaloid found in kinds of Sophora plants mainly including Sophora flavescens, Sophora alopecuroides and Sophora subprotrata. The aim of the present study was to evaluate therapeutic effects of matrine on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Two hours following colonic instillation of TNBS, matrine with several doses was given by gastric gavage once daily for 7 days. Comparing with the 0.9% NaCl-treated mice with TNBS-induced colitis, matrine (10 and 20 mg kg(-1))-treated mice with TNBS-induced colitis were shown improvements of weight loss, macroscopic score, histological score, and myeloperoxidase (MPO) activity. Moreover, treatments with matrine (10 and 20 mg kg(-1)) decreased the up-regulated mRNA and protein levels of tumour necrosis factor-alpha (TNF-alpha) caused by TNBS. Our findings suggest that matrine improves TNBS-induced colitis in mice and the therapeutic mechanism might be related to the reduction of up-regulated colonic TNF-alpha production caused by TNBS.
authors.affiliation: Department of Internal Medicine & Geriatrics, Zhongnan Hospital, Research Center of Digestive Diseases, Wuhan University School of Medicine, Wuhan 430071, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cheng
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Ye
authors.forename: Zheng-Guo
authors.initials: ZG
authors.lastname: Hu
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Wang
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.forename: Qiu-Sha
authors.initials: QS
authors.lastname: Guo
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D011807:Quinolizines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014302:Trinitrobenzenesulfonic Acid; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D011807:Quinolizines; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase; D000093842:Matrines