title: [Isolation, induction and differentiation of human blood-derived endothelia progenitor cells].
abstract: OBJECTIVE
  To determine the biological traits and optimal condition for the induction and differentiation of endothelial progenitor cells from peripheral blood in healthy adults.
  
  
  METHODS
  Mononuclear cells isolated from peripheral blood of healthy adults were cultured in M199 medium supplemented with VEGF, bFGF, IGF-1, and EGF. The appearing time of cell clusters or spindle-shaped cells was recorded respectively. Attached spindle-shaped cells were detached and labeled with a series of antibodies against blood vessel endothelial-specific markers.
  
  
  RESULTS
  Attached spindle-like cells appeared 4 days after the culture, cell clusters were observed at 5 to 8 days, and cord-like structure was formed by 10th day. These cells expressed endothelial-specific markers.
  
  
  CONCLUSIONS
  Endothelial progenitors cells were derived from mononuclear cells of peripheral blood, which can be induced into endothelial cells at specific conditions.
authors.affiliation: Departement of Endocrinology, Xiangya Hospital, Xiangya Hospital, Central South University, Changsha, China. medicalliu@hotmail.com
authors.forename: Ze-Hao
authors.initials: ZH
authors.lastname: Liu
authors.forename: Min-Xiang
authors.initials: MX
authors.lastname: Lei
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Wang
authors.forename: Zuo-Xiong
authors.initials: ZX
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: San-E
authors.initials: SE
authors.lastname: Tang
mesh_terms: D002454:Cell Differentiation; D002469:Cell Separation; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*