A general solid phase method for the preparation of diverse azapeptide probes directed against cysteine proteases.
[chemical reaction: see text]. A solid phase approach is presented for the synthesis of azapeptide inhibitors and activity based probes (ABPs) for cysteine proteases. This synthetic method allows the incorporation of diverse reactive warheads linked to different peptide recognition elements. Application of this method to the synthesis of a series of caspase probes is described.
Kato
Daisuke
D
Department of Pathology, Stanford School of Medicine, Stanford, California 94305, USA.
Verhelst
Steven H L
SH
Sexton
Kelly B
KB
Bogyo
Matthew
M
D000588:Amines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001372:Aza Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D020650:Combinatorial Chemistry Techniques; D015853:Cysteine Proteinase Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D008872:Microwaves; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology
D000588:Amines; D001372:Aza Compounds; D015853:Cysteine Proteinase Inhibitors; D015335:Molecular Probes; D010455:Peptides