title: Expressed sequence tags from the midgut and an epithelial cell line of Chironomus tentans: annotation, bioinformatic classification of unknown transcripts and analysis of expression levels.
abstract: Expressed sequence tags (ESTs) were generated from two Chironomus tentans cDNA libraries, constructed from an embryo epithelial cell line and from larva midgut tissue. 8584 5'-end ESTs were generated and assembled into 3110 tentative unique transcripts, providing the largest contribution of C. tentans sequences to public databases to date. Annotation using Blast gave 1975 (63.5%) transcripts with a significant match in the major gene/protein databases, 1170 with a best match to Anopheles gambiae and 480 to Drosophila melanogaster. 1091 transcripts (35.1%) had no match to any database. Studies of open reading frames suggest that at least 323 of these contain a coding sequence, indicating that a large proportion of the genes in C. tentans belong to previously unknown gene families.
authors.affiliation: Stockholm Bioinformatics Center, Abanova University Center, Royal Institute of Technology (KTH), Stockholm, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Arvestad
authors.forename: N
authors.initials: N
authors.lastname: Visa
authors.forename: J
authors.initials: J
authors.lastname: Lundeberg
authors.forename: L
authors.initials: L
authors.lastname: Wieslander
authors.forename: P
authors.initials: P
authors.lastname: Savolainen
mesh_terms: D000818:Animals; D002460:Cell Line; D002683:Chironomidae / Q000235:genetics*; D019295:Computational Biology; D004847:Epithelial Cells / Q000378:metabolism*; D020224:Expressed Sequence Tags; D041981:Gastrointestinal Tract / Q000166:cytology; D020869:Gene Expression Profiling; D019476:Insect Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D019476:Insect Proteins