title: Molecular characterization of Theileria species associated with mortality in four species of African antelopes.
abstract: Pathogen DNA was isolated from roan antelope (Hippotragus equinus), sable antelope (Hippotragus niger), greater kudu (Tragelaphus strepsiceros), and common gray duiker (Sylvicapra grimmia) in South Africa whose deaths were attributed to either theileriosis or cytauxzoonosis. We developed Theileria species-specific probes used in combination with reverse line blot hybridization assays and identified three different species of Theileria in four African antelope species. The close phylogenetic relationship between members of the genera Theileria and Cytauxzoon, similarities in the morphologies of developmental stages, and confusion in the literature regarding theileriosis or cytauxzoonosis are discussed.
authors.affiliation: Division of Parasitology and Tropical Veterinary Medicine, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands. A.Nijhof@vet.uu.nl
authors.forename: A M
authors.initials: AM
authors.lastname: Nijhof
authors.forename: V
authors.initials: V
authors.lastname: Pillay
authors.forename: J
authors.initials: J
authors.lastname: Steyl
authors.forename: L
authors.initials: L
authors.lastname: Prozesky
authors.forename: W H
authors.initials: WH
authors.lastname: Stoltsz
authors.forename: J A
authors.initials: JA
authors.lastname: Lawrence
authors.forename: B L
authors.initials: BL
authors.lastname: Penzhorn
authors.forename: F
authors.initials: F
authors.lastname: Jongejan
mesh_terms: D000818:Animals; D000866:Antelopes / Q000469:parasitology*; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D016794:Theileria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D013801:Theileriasis / Q000401:mortality* / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan; D015345:Oligonucleotide Probes