Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using monoclonal antibody against ginsenoside Re.
A chromatographic immunostaining method has been developed for the determination of ginsenoside Re (G-Re) in ginseng samples on a polyethersulphone (PES) membrane. G-Re standard and the extracts of ginseng roots were applied to a PES membrane and developed by methanol-water-acetic acid (45:55:1, by volume). G-Re was clearly detected by an immunostaining method using a monoclonal antibody against G-Re. The coloring spots of G-Re were analyzed quantitatively using NIH Image software indicating at least 0.125 microg of G-Re was detectable. G-Re can be analyzed quantitatively between 0.25 and 4.0 microg.
Morinaga
Osamu
O
Innovation Plaza Fukuoka, Japan Science and Technology Agency, Sawara-ku, Fukuoka.
Tanaka
Hiroyuki
H
Shoyama
Yukihiro
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002855:Chromatography, Thin Layer / Q000379:methods*; D005260:Female; D036145:Ginsenosides / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D005894:Panax / Q000737:chemistry*; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D036145:Ginsenosides; C049864:ginsenoside Re