[Phalloidin plays an important role in calcium ionophore A23187-induced human acrosome reaction].
OBJECTIVE
To discuss the important role of actin polymerization in calcium ionophore A23187-induced human acrosome reaction and its mechanism.


METHODS
Each spermatozoon specimen was divided into five groups, treated with A23187 3 micromol/L in Group A, Phalloidin 40 micromol/L and A23187 3 micromol/L in Group B, SLO 0.5 U/ml and A23187 3 micromol/L in Group C, SLO 0.5 U/ ml, Phalloidin 40 micromol/L and A23187 3 micromol/L in Group D, and nothing added in Grpup E. Then the percentage of the human acrosome reaction was assessed with Rhodamine-PSA (10 microg/ml).


RESULTS
The difference of the human spermatozoon acrosome reaction was significant (P < 0.01) among the 5 groups with or without SLO, Phalloidin and calcium ionophore A23187 but not between Groups A and B (P > 0.01).


CONCLUSIONS
Phalloidin does not work on the acrosome reaction of intact human spermatozoa, but in an SLO-permeabilized human spermatozoal model, it can obviously decrease the percentage of human spermatozoon acrosome reaction, which indicates that the polymerization of actin plays an important role in the course of human spermatozoon acrosome reaction, and mostly acts on the acrosome inside.
Wu
Shuai
S
Department of Urology, Qingdao People's Hospital, Affiliated Hospital of Qingdao Medical University, Qingdao, Shandong 266003, China. wsmail@ruyi.com
Wang
Xin-Sheng
XS
Meng
Fan-Hui
FH
D020101:Acrosome Reaction / Q000187:drug effects*; D000199:Actins / Q000502:physiology; D001426:Bacterial Proteins / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology*; D002478:Cells, Cultured; D006801:Humans; D007476:Ionophores / Q000494:pharmacology*; D008297:Male; D010590:Phalloidine / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013301:Streptolysins / Q000494:pharmacology
D000199:Actins; D001426:Bacterial Proteins; D007476:Ionophores; D013301:Streptolysins; C025964:streptolysin O; D010590:Phalloidine; D000001:Calcimycin