title: Effects of decreased resource availability, protozoan grazing and viral impact on a structure of bacterioplankton assemblage in a canyon-shaped reservoir.
abstract: We conducted a transplant experiment to elucidate the effects of different levels of grazing pressure, nutrient availability, especially phosphorus, and the impact of viruses on the changes in the structure of bacterioplankton assemblage in a meso-eutrophic reservoir. A sample taken from the nutrient-rich inflow part of the reservoir was size-fractionated and incubated in dialysis bags in both inflow and dam area. The structure of bacterial assemblage was examined by fluorescence in situ hybridization using oligonucleotide probes with different levels of specificity. In terms of the relative proportions of different bacterial groups, we found very few significant changes in the bacterioplankton composition after transplanting the treatments to the nutrient-poor dam area. However, we observed marked shifts in morphology and biomass towards the development of filaments, flocs and "vibrio-like" morphotypes of selected probe-defined groups of bacteria induced by increased grazing pressure. Despite the very high abundances of viruses in all the treatments, their effects on bacterioplankton were rather negligible.
authors.affiliation: Hydrobiological Institute of the Academy of Sciences of the Czech Republic, Na Sádkách 7, CZ-370 05 Ceské Budejovice, Czech Republic. hornak@hbu.cas.cz
authors.forename: Karel
authors.initials: K
authors.lastname: Hornák
authors.forename: Michal
authors.initials: M
authors.lastname: Masín
authors.forename: Jan
authors.initials: J
authors.lastname: Jezbera
authors.forename: Yvan
authors.initials: Y
authors.lastname: Bettarel
authors.forename: Jirí
authors.initials: J
authors.lastname: Nedoma
authors.forename: Télesphore
authors.initials: T
authors.lastname: Sime-Ngando
authors.forename: Karel
authors.initials: K
authors.lastname: Simek
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000821:virology; D001435:Bacteriophages / Q000302:isolation & purification / Q000502:physiology*; D004269:DNA, Bacterial / Q000032:analysis; D017753:Ecosystem; D056890:Eukaryota / Q000254:growth & development / Q000502:physiology*; D017404:In Situ Hybridization, Fluorescence; D008242:Lysogeny; D008969:Molecular Sequence Data; D010802:Phylogeny; D010933:Plankton / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000821:virology; D011235:Predatory Behavior / Q000502:physiology*; D045483:Rivers / Q000737:chemistry / Q000382:microbiology / Q000469:parasitology / Q000821:virology; D014881:Water Supply
chemical_list: D004269:DNA, Bacterial