Effects of additional Vitamin E and selenium supply on G6PDH activity in rats treated with high doses of glucocorticoid.
The aim of this work was to determine the effects of dietary intake Vitamin E and selenium (Se) on glucose-6-phosphate dehydrogenase (G6PDH) activity in rats treated with high doses of prednisolone. Two hundred and fifty adult male Wistar rats were randomly divided into five groups. The rats were fed a normal diet, but groups 3, 4, and 5 received a daily supplement in their drinking water of 20mg Vitamin E, 0.3mg Se, and a combination of Vitamin E and Se, respectively, for 30 days. For 3 days subsequently, the control group (group 1) was treated with a placebo, and the remaining four groups were injected intramuscularly with 100 mg/kg body weight prednisolone. After the last administration of prednisolone, 10 rats from each group were killed at 4, 8, 12, 24, and 48 h and the activities of G6PDH enzymes in their tissues were measured. Hepatic and spleen G6PDH activities in the prednisolone treatment group began to decrease gradually at 8 h, while enzyme activities did not change in the kidney and heart. However, the administration of Vitamin E alone did not affect G6PDH activity in any of the tissues. Se supplementation had a preventive effect on the decrease of G6PDH caused by prednisolone and improved the diminished activities of G6PDH. Therefore, the present study demonstrates that a high dose of prednisolone may alter the effects of normal dose glucocorticoids and that Se is effective in reducing damage in prednisolone-treated rats. Se may prevent the changes in G6PDH activity in various tissues caused by prednisolone in various tissues.
Yilmaz
Seval
S
Department of Biochemistry, Faculty of Veterinary Medicine, College of Veterinary Medicine, Firat (Euphrates) University, Elazig 23119, Turkey. sevyilars@yahoo.com
Beytut
Ebru
E
Erişir
Mine
M
Ozan
Sema
S
Aksakal
Mesut
M
D000818:Animals; D004338:Drug Combinations; D004347:Drug Interactions; D005526:Food, Formulated; D005938:Glucocorticoids / Q000008:administration & dosage*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007273:Injections, Intramuscular / Q000379:methods; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000201:enzymology; D013997:Time Factors; D014810:Vitamin E / Q000494:pharmacology*
D004338:Drug Combinations; D005938:Glucocorticoids; D014810:Vitamin E; D005954:Glucosephosphate Dehydrogenase; D012643:Selenium