P2Y isoforms operative in mouse taste cells.
Recent functional evidence indicates that mouse taste cells express P2Y receptors coupled to IP(3) production and Ca(2+) mobilization. Our studies of the expression profile of particular P2Y isoforms in the taste tissue of the mouse have revealed that ATP and UTP equipotently mobilize intracellular Ca(2+) at saturating concentrations, suggesting that common receptors for both nucleotides, i.e., P2Y(2) and P2Y(4) subtypes, might be involved. Reverse transcription/polymerase chain reaction and immunohistochemistry have confirmed the presence of P2Y(2) and P2Y(4) receptors in a population of taste bud cells from the circumvallate and foliate papillae. Transcripts for the P2Y(1) and P2Y(6) isoforms have also been detected in taste tissue preparations, this observation being consistent with the ADP and UDP responsiveness of taste cells. Together, our data suggest that P2Y(2) and P2Y(4) receptors play a predominant role in mediating taste cell responses to ATP and UTP.
Bystrova
Marina F
MF
Institute of Cell Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia. staskolesnikov@yahoo.com
Yatzenko
Yulia E
YE
Fedorov
Ilya V
IV
Rogachevskaja
Olga A
OA
Kolesnikov
Stanislav S
SS
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D020033:Protein Isoforms / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D013650:Taste Buds / Q000166:cytology / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000378:metabolism*
D020033:Protein Isoforms; D018048:Receptors, Purinergic P2; D000255:Adenosine Triphosphate; D002118:Calcium; D014544:Uridine Triphosphate