Glycosidation of cu,zn-superoxide dismutase with end-group aminated dextran: pharmacological and pharmacokinetics properties.
Bovine Cu,Zn-SOD was chemically modified with an end-group aminated dextran derivative using a water-soluble carbodiimide as coupling agent. The enzyme retained 81% of the initial catalytic activity after the attachment of about 4.4 mol of polymer per protein subunit. The anti-inflammatory activity of the SOD was two times increased after conjugation with dextran. The modified enzyme was remarkably more resistant to inactivation by H(2)O(2) and its plasma half-life time was prolonged from 4 min to 3.2 h.
Perez
Yunel
Y
Enzyme Technology Group, University of Matanzas, Autopista a Varadero km 3 1/2, Matanzas, C.P. 44740, Cuba.
Valdivia
Aimara
A
Gomez
Leissy
L
Simpson
Benjamin K
BK
Villalonga
Reynaldo
R
D000588:Amines / Q000737:chemistry; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D019540:Area Under Curve; D001224:Aspartic Acid / Q000737:chemistry; D002240:Carbohydrate Sequence; D003911:Dextrans / Q000737:chemistry*; D004487:Edema / Q000188:drug therapy; D004789:Enzyme Activation; D005534:Foot Diseases / Q000188:drug therapy; D005960:Glucosides / Q000378:metabolism; D018698:Glutamic Acid / Q000737:chemistry; D006207:Half-Life; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*
D000588:Amines; D000894:Anti-Inflammatory Agents, Non-Steroidal; D003911:Dextrans; D005960:Glucosides; D001224:Aspartic Acid; D018698:Glutamic Acid; D013482:Superoxide Dismutase