Use of microelectronic array technology for rapid identification of clinically relevant mycobacteria.
We developed a new method based on the Nanochip microelectronic array technology for identification of various clinically relevant mycobacterial species. PCR-amplified rRNA genes obtained from 270 positive Mycobacteria Growth Indicator Tube cultures were successfully tested by hybridizing them with species-selective probes, and the results agreed with those of conventional identification methods. The system is rapid and accurate and opens new perspectives in clinical diagnostics.
Sanguinetti
Maurizio
M
Istituto di Microbiologia, Largo F. Vito 1, 00168 Rome, Italy. msanguinetti@rm.unicatt.it
Novarese
Linda
L
Posteraro
Brunella
B
Ranno
Stefania
S
De Carolis
Elena
E
Pecorini
Giovanni
G
Lucignano
Barbara
B
Ardito
Fausta
F
Fadda
Giovanni
G
D015373:Bacterial Typing Techniques; D006801:Humans; D056656:Lab-On-A-Chip Devices; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D009164:Mycobacterium Infections / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D036103:Nanotechnology; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013997:Time Factors; D014376:Tuberculosis / Q000382:microbiology
D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S