title: Separation and purification of chlorogenic acid by molecularly imprinted polymer monolithic stationary phase.
abstract: Separation and purification of chlorogenic acid by removal of the impurities compounds co-existed in the product using molecular imprinting technique was firstly reported. In this work, an in situ synthesis method was utilized for the preparation of molecularly imprinted polymer monolithic stationary phase using the impurity molecule (caffeic acid) as template, the mixture of tetrahydrofuran and isooctane as solvent, and methacrylic acid and ethyl glycol dimethacrylate as functional monomer and cross-linker, respectively. The retention behavior of the monolithic polymer to chlorogenic acid molecule, the template and several main impurities compounds in the product was studied and the adsorption capacity of compounds on the stationary phase determined by frontal chromatographic technique. A relatively weak retention of the target product molecule (chlorogenic acid) on the polymer and a strong adsorption capability of the monolith to the template and several main impurities were observed. This might mainly result from the 'shape' difference of chlorogenic acid molecule with the impurities compounds molecules. This approach was shown to be successful for the separation and purification of chlorogenic acid from the extract of Eucommia ulmodies leaves.
authors.affiliation: State Key Laboratory of Chemo/Bio Sensing & Chemometrics, Hunan University, Changsha 410082, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Yingju
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhang
authors.forename: Haiping
authors.initials: H
authors.lastname: Liao
authors.forename: Lihua
authors.initials: L
authors.lastname: Nie
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D000327:Adsorption; D002109:Caffeic Acids / Q000737:chemistry; D002726:Chlorogenic Acid / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D031283:Eucommiaceae / Q000737:chemistry; D005663:Furans / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D009795:Octanes / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*
chemical_list: D002109:Caffeic Acids; D003432:Cross-Linking Reagents; D005663:Furans; D008689:Methacrylates; D009795:Octanes; D011108:Polymers; C008384:methacrylic acid; D002726:Chlorogenic Acid; C018674:tetrahydrofuran; C045798:2,2,4-trimethylpentane; C040048:caffeic acid