Mesenchymal stem cells differentiate into tenocytes by bone morphogenetic protein (BMP) 12 gene transfer.
Rhesus bone marrow mesenchymal stem cells (MSCs) were transfected with the BMP12 gene by electroporation, and the phenotype of the transfected cells was identified by morphological observation and molecular biological assay. After transfection, cells became slender, and their processes became thinner and were interwoven into a network. There were more organelles in the transfected cells than in the parental MSCs. The transfected cells exhibited mRNA expressions of BMP12, collagen type I and scleraxis, but not collagen type III mRNA expression. Immunocytochemical analysis also showed the presence of collagen type I, but not collagen type III in the transfected cells. The transfected cells were positive for CD44 and negative for HLA-DR. Therefore, MSCs can be introduced to differentiate into tenocytes by BMP12 gene transfection, and bone marrow MSCs can serve as an alternative seed cell for application in tendon tissue engineering.
Wang
Qi-Wei
QW
Department of Cell Engineering, Institute of Biotechnology, 20 Dongda Street, Fengtai, Beijing 100071, P.R. China. wangqiwei2001@yahoo.com.cn
Chen
Zhao-Lie
ZL
Piao
Ying-Jie
YJ
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D018014:Gene Transfer Techniques; D055412:Growth Differentiation Factors; D006801:Humans; D008253:Macaca mulatta; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D013710:Tendons / Q000166:cytology*; D014162:Transfection
D051792:Basic Helix-Loop-Helix Proteins; D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I; D055412:Growth Differentiation Factors; D012333:RNA, Messenger; C499470:GDF7 protein, human