Global Analysis of the Hortaea werneckii proteome: studying steroid response in yeast.
The response of the halophilic black yeast Hortaea werneckii to the steroid hormone progesterone has been studied at the protein level using fluorescent two-dimensional differential gel electrophoresis (2D-DIGE) technology in combination with mass spectrometry. Data on protein identification from this study reveal molecular mechanisms of the response to progesterone. In particular, the overexpression of Pck2 and Pac2 in the stimulated cells indicates the interactions of progesterone with the cell growth and reproduction signaling pathways.
Matis
Maja
M
Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912, USA.
Zakelj-Mavric
Marija
M
Peter-Katalinić
Jasna
J
D001203:Ascomycota / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000737:chemistry*; D005786:Gene Expression Regulation; D013058:Mass Spectrometry; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D011374:Progesterone / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence; D013256:Steroids / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D015854:Up-Regulation
D018076:DNA, Complementary; D005656:Fungal Proteins; D011506:Proteins; D020543:Proteome; D013256:Steroids; D011374:Progesterone