title: Expression of inflammatory cytokines in pigs experimentally infected with Mycoplasma hyopneumoniae.
abstract: The expression of interleukin-1 (IL-1), tumour necrosis factor-alpha (TNF-alpha) and IL-6 were studied over a period of 35 days in the lungs of pigs experimentally infected with Mycoplasma hyopneumoniae, by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), morphometric analysis and in-situ hybridization. Fifteen colostrum-deprived pigs aged 14 days were inoculated intranasally with M. hyopneumoniae. IL-1, TNF-alpha and IL-6 were detected by RT-PCR in the lungs of the infected pigs from 7 days post-inoculation (dpi) onwards, but not in the uninfected control pigs. Concurrent expression of all three cytokines was always observed, in association with lung lesions. Inflammatory cytokine-positive cells were detected in the lungs at 7 dpi, their number increasing at 21dpi, and decreasing thereafter. The results suggest that such cytokines play a role in mediating and regulating inflammation in M. hyopneumoniae infection.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1, Shillim-Dong, Kwanak-Gu 151-742, Seoul.
authors.forename: C
authors.initials: C
authors.lastname: Choi
authors.forename: D
authors.initials: D
authors.lastname: Kwon
authors.forename: K
authors.initials: K
authors.lastname: Jung
authors.forename: Y
authors.initials: Y
authors.lastname: Ha
authors.forename: Y-H
authors.initials: YH
authors.lastname: Lee
authors.forename: O
authors.initials: O
authors.lastname: Kim
authors.forename: H-K
authors.initials: HK
authors.lastname: Park
authors.forename: S-H
authors.initials: SH
authors.lastname: Kim
authors.forename: K-K
authors.initials: KK
authors.lastname: Hwang
authors.forename: C
authors.initials: C
authors.lastname: Chae
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology*; D013552:Swine; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha