title: Antioxidative activity of microencapsulated gamma-oryzanol on high cholesterol-fed rats.
abstract: The effectiveness of microencapsulated gamma-oryzanol (M-gamma-OZ) was evaluated as an antioxidant in Sprague-Dawley rats. Lard containing 100 ppm of gamma-OZ (HCD III) or 100 ppm of M-gamma-OZ (HCD IV) was heated in an oven for 7 days, and the heat-treated lard as an ingredient in a high cholesterol diet (HCD) formulation was tested for analyzing in vivo cholesterol and lipid profiles. The HCDs containing fresh lard (HCD I) and heat-treated lard (HCD II) were fed to the rats for 4 weeks as control groups A and B, respectively, in this experiment. The liver thiobarbituric acid reactive substances values of group C (fed with HCD III) and group D (with HCD IV) were significantly lower (p < 0.05) than that of negative control, group B. One of the cholesterol oxidation products, 7-ketocholesterol, was not detected from group D, indicating that microencapsulation preserved antioxidative activity effectively. The levels of serum total cholesterol and lipoproteins, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein were also affected by heat-induced lipid oxidation.The M-gamma-OZ evidently decreased LDL-cholesterol content and increased HDL-cholesterol in blood samples of tested rats. These results suggested that the M-gamma-OZ was not only effective in inhibiting the hypercholesterolemia of serum and liver but also reduced the oxidation degree of lipids and cholesterol. Therefore, this microencapsulation can be a good potential technique to protect the antioxidant activity of gamma-OZ from heat-induced lipid oxidation.
authors.affiliation: Department of Food and Nutrition, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Korea.
authors.forename: Mun-Hee
authors.initials: MH
authors.lastname: Suh
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Yoo
authors.forename: Pahn-Shick
authors.initials: PS
authors.lastname: Chang
authors.forename: Hyeon Gyu
authors.initials: HG
authors.lastname: Lee
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D002784:Cholesterol / Q000032:analysis / Q000097:blood; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D004339:Drug Compounding; D007653:Ketocholesterols / Q000032:analysis / Q000097:blood; D008074:Lipoproteins / Q000097:blood; D008099:Liver / Q000737:chemistry; D008297:Male; D010666:Phenylpropionates / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014280:Triglycerides / Q000097:blood
chemical_list: D000975:Antioxidants; D002791:Cholesterol, Dietary; D007653:Ketocholesterols; D008074:Lipoproteins; D010666:Phenylpropionates; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D002784:Cholesterol; C003001:7-ketocholesterol; C013172:gamma-oryzanol