title: Analysis of antibiotics in urine and wipe samples from environmental and biological monitoring--comparison of HPLC with UV-, single MS- and tandem MS-detection.
abstract: Results of the simultaneous determination of the structurally different antibiotics cefazoline, cefotiame, cefuroxime, chloramphenicol, ciprofloxacin, ofloxacin, sulfamethoxazole and trimethoprim from environmental and biological monitoring using high-performance liquid chromatography with UV, single mass and tandem mass spectrometry were compared. For sample enrichment and clean-up a SPE method using bakerbond C18 cartridges was developed. Mean recovery rates were above 70%. Because of the complex urine matrix, only the wipe samples could be analyzed by UV-detection. However, UV-detection and single MS-detection are useful for control measurements after spillage, e.g. (LOD=1-2 ng/cm(2)). Samples from biological monitoring of occupational uptake should be analyzed by LC-MS/MS. The limits of detection (LOD) in urine ranged from 0.4 to 70 microg/L for LC-MS and 0.01 to 0.9 microg/L for LC-MS/MS detection. The limits of detection in wipe samples ranged from 0.003 to 0.13 ng/cm(2).
authors.affiliation: Institute of Energy and Environmental Technology (IUTA), Bliersheimer Strasse 60, D-47229 Duisburg, Germany. tuerk@iuta.de
authors.forename: Jochen
authors.initials: J
authors.lastname: Tuerk
authors.forename: Marius
authors.initials: M
authors.lastname: Reinders
authors.forename: Dennis
authors.initials: D
authors.lastname: Dreyer
authors.forename: Thekla K
authors.initials: TK
authors.lastname: Kiffmeyer
authors.forename: Klaus Gerhard
authors.initials: KG
authors.lastname: Schmidt
authors.forename: Heinz-Martin
authors.initials: HM
authors.lastname: Kuss
mesh_terms: D000900:Anti-Bacterial Agents / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004781:Environmental Exposure / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents