title: On-bead fluorescence assay for serine/threonine kinases.
abstract: [chemical reaction: see text]. A novel fluorescence-based assay for serine/threonine kinases is described. Base-mediated beta-elimination of the phosphate moiety and the Michael addition of a thiol-containing fluorescent molecule allows convenient and efficient detection of the enzyme activity. This approach may be broadly applicable to various serine/threonine kinases.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Nagoya, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Akita
authors.forename: Naoki
authors.initials: N
authors.lastname: Umezawa
authors.forename: Tsunehiko
authors.initials: T
authors.lastname: Higuchi
mesh_terms: D000595:Amino Acid Sequence; D047388:Casein Kinases / Q000032:analysis / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes; D015394:Molecular Structure; D010766:Phosphorylation; D011494:Protein Kinases / Q000032:analysis / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000032:analysis* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D005456:Fluorescent Dyes; D011494:Protein Kinases; D047388:Casein Kinases; D017346:Protein Serine-Threonine Kinases