High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts.
A tobacco chloroplast expression vector, pTRVP1, containing the foot-and-mouth disease virus (FMDV) VP1 gene and the selective marker aadA gene, was constructed and transferred to tobacco by biolistic method. Three resistant lines were obtained through spectinomycin selection, and each transgenic line was subjected to a second round of spectinomycin selection. PCR and PCR southern blot analysis revealed that the VP1 gene had integrated into the chloroplast genome. Western blot and quantification ELISA assays indicated that the VP1 gene was expressed in tobacco chloroplasts and accounted for 2-3% of total soluble protein. This suggested that plant chloroplasts were an efficient expression system for the potential production of recombinant antigens in plants.
Li
Yinü
Y
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
Sun
Meng
M
Liu
Jixing
J
Yang
Zongqi
Z
Zhang
Zhifang
Z
Shen
Guifang
G
D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism*; D002736:Chloroplasts / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics*; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000166:cytology* / Q000821:virology*
D036022:Capsid Proteins; C442501:VP1 protein, Foot-and-mouth disease virus