Protein carbonylation and heat shock response in Ruditapes decussatus following p,p'-dichlorodiphenyldichloroethylene (DDE) exposure: a proteomic approach reveals that DDE causes oxidative stress.
Protein carbonylation and levels of heat shock proteins (hsp; 60, 70 and 90 kDa) were measured in gill, mantle and digestive gland of Ruditapes decussatus following exposure to p,p'-dichlorodiphenyldichloroethylene (DDE). Heat shock response was measured by immunoblotting using antibodies specific to heat shock proteins (hsps). Densitometry analysis of individual bands revealed no difference between control and treated samples except appearance of hsp90 in DDE-treated mantle. Carbonylated protein content was determined following 2,4-dinitrophenylhydrazine derivatization and two-dimensional electrophoresis coupled with western blotting. Immunoblotting with dinitrophenol-specific antibody revealed extensive differences in both extent and number of carbonylated proteins in mantle and digestive gland in response to DDE while gill was unaffected. These results demonstrate for the first time that DDE causes tissue-specific formation of reactive oxygen species in clams.
Dowling
Vera
V
Proteomics Research Group, Department of Biochemistry, University College Cork, Ireland.
Hoarau
Pascal C
PC
Romeo
Michèle
M
O'Halloran
John
J
van Pelt
Frank
F
O'Brien
Nora
N
Sheehan
David
D
D000818:Animals; D000906:Antibodies / Q000276:immunology; D049872:Bivalvia / Q000187:drug effects*; D003720:Densitometry / Q000379:methods; D003633:Dichlorodiphenyl Dichloroethylene / Q000633:toxicity*; D004064:Digestive System / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004140:Dinitrophenols / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods; D005880:Gills / Q000187:drug effects; D018869:Heat-Shock Response / Q000187:drug effects*; D015151:Immunoblotting / Q000662:veterinary; D007306:Insecticides / Q000633:toxicity*; D018384:Oxidative Stress; D050050:Protein Carbonylation / Q000187:drug effects*; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000032:analysis
D000906:Antibodies; D004140:Dinitrophenols; D007306:Insecticides; D017382:Reactive Oxygen Species; D003633:Dichlorodiphenyl Dichloroethylene; D004121:Dimethyl Sulfoxide