title: Validation and clinical application of molecular methods for the identification of molds in tissue.
abstract: BACKGROUND
  Invasive fungal infections due to less-common molds are an increasing problem, and accurate diagnosis is difficult.
  
  
  METHODS
  We used our previously established molecular method, which allows species identification of molds in histological tissue sections, to test sequential specimens from 56 patients with invasive fungal infections who were treated at our institution from 1982 to 2000.
  
  
  RESULTS
  The validity of the method was demonstrated with the establishment of a molecular diagnosis in 52 cases (93%). Confirmation of the causative organism was made in all cases in which a mold had been cultured from the tissue specimen. Less-common molds were identified in 7% of cases and appear to be an increasing problem.
  
  
  CONCLUSIONS
  Our previously established method has proven to be of value in determining the incidence of invasive infection caused by less-common molds. Institutions should continue to pursue diagnosis of invasive fungal infections by means of tissue culture and microbiologic analysis.
authors.affiliation: Department of Medical Microbiology, Royal Free and University College Medical School, Royal Free Hospital, London, United Kingdom.
authors.forename: P J
authors.initials: PJ
authors.lastname: Paterson
authors.forename: S
authors.initials: S
authors.lastname: Seaton
authors.forename: T D
authors.initials: TD
authors.lastname: McHugh
authors.forename: J
authors.initials: J
authors.lastname: McLaughlin
authors.forename: M
authors.initials: M
authors.lastname: Potter
authors.forename: H G
authors.initials: HG
authors.lastname: Prentice
authors.forename: C C
authors.initials: CC
authors.lastname: Kibbler
mesh_terms: D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology*; D001231:Aspergillus flavus / Q000235:genetics / Q000302:isolation & purification*; D001232:Aspergillus fumigatus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques