Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry.
Glycoproteins in cerebrospinal fluid are found to be altered in Alzheimer patients compared to healthy control individuals. We have utilized micro-solution isoelectric focusing and affinity chromatography, prior to gel electrophoresis to enable site-specific structural determination of the N-linked glycans in apolipoprotein J with the use of FT-ICR MS. The albumin depletion method is the most suitable as prefractionation method of CSF prior to 2-DE for structural determination of glycoproteins in the study of neurodegenerative disorders.
Sihlbom
Carina
C
Institute of Medical Biochemistry, Göteborg University, Sweden.
Davidsson
Pia
P
Nilsson
Carol L
CL
D000418:Albumins / Q000737:chemistry; D000544:Alzheimer Disease / Q000378:metabolism*; D002555:Cerebrospinal Fluid / Q000378:metabolism*; D002846:Chromatography, Affinity; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006020:Glycopeptides / Q000737:chemistry; D006023:Glycoproteins / Q000134:cerebrospinal fluid* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000737:chemistry; D020033:Protein Isoforms; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014357:Trypsin / Q000737:chemistry
D000418:Albumins; D006020:Glycopeptides; D006023:Glycoproteins; D011134:Polysaccharides; D020033:Protein Isoforms; D014357:Trypsin