title: Evaluation of the LightCycler Staphylococcus M GRADE kits on positive blood cultures that contained gram-positive cocci in clusters.
abstract: We evaluated the Roche LightCycler Staphylococcus M(GRADE) kits to differentiate between Staphylococcus aureus and coagulase-negative staphylococci in blood cultures growing clusters of gram-positive cocci. Testing 100 bottles (36 containing S. aureus), the assay was 100% sensitive and 98.44% specific for S. aureus and 100% sensitive and specific for coagulase-negative staphylococci.
authors.affiliation: Department of Infectious Diseases, The Cleveland Clinic Foundation, 9500 Euclid Avenue, S-32, Cleveland, OH 44195, USA. shrestn@ccf.org
authors.forename: Nabin K
authors.initials: NK
authors.lastname: Shrestha
authors.forename: Marion J
authors.initials: MJ
authors.lastname: Tuohy
authors.forename: Ravindran A
authors.initials: RA
authors.lastname: Padmanabhan
authors.forename: Gerri S
authors.initials: GS
authors.lastname: Hall
authors.forename: Gary W
authors.initials: GW
authors.lastname: Procop
mesh_terms: D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology*; D003030:Coagulase / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D006095:Gram-Positive Cocci / Q000145:classification* / Q000254:growth & development* / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013210:Staphylococcus / Q000145:classification / Q000201:enzymology / Q000254:growth & development; D013211:Staphylococcus aureus / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification
chemical_list: D003030:Coagulase; D003470:Culture Media; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic