Characterization of nigerlysin, hemolysin produced by Aspergillus niger, and effect on mouse neuronal cells in vitro.
Aspergillus niger produced a proteinaceous hemolysin, nigerlysin when incubated on sheep's blood agar (SBA) at both 23 and 37 degrees C. Nigerlysin was purified from tryptic soy broth (TSB) culture filtrate and found to have a molecular weight of approximately 72 kDa, with an isoelectric point of 3.45. Nigerlysin is heat stable up to 65 degrees C but unstable at 75 degrees C when incubated for 10 min. Circular dichroic analysis revealed that nigerlysin has an alpha helical structure. Exposure of mouse primary cortical neuronal cells to 0.1 microg ml(-1) of nigerlysin resulted in the rapid loss of their viability, approximately 50% in 24 h. The IC50 is estimated to be 0.037 microg ml(-1), or between 0.034 and 0.041 microg ml(-1) at the 95% confidence level.
Donohue
Maura
M
National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, OH, USA.
Wei
Wei
W
Wu
Jinfang
J
Zawia
Nasser H
NH
Hud
Nicholas
N
De Jesus
Victor
V
Schmechel
Detlef
D
Hettick
Justin M
JM
Beezhold
Donald H
DH
Vesper
Stephen
S
D000818:Animals; D001234:Aspergillus niger / Q000187:drug effects / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002942:Circular Dichroism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000138:chemical synthesis* / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D020953:Neuroaspergillosis; D009474:Neurons / Q000187:drug effects*; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005656:Fungal Proteins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins