The Saccharomyces cerevisiae phosphatase activator RRD1 is required to modulate gene expression in response to rapamycin exposure.
We show that mutants lacking either the phosphatase activator Rrd1 or the phosphatase Pph3 are resistant to rapamycin and that double mutants exhibit a synergistic response. This phenotype could be related to an inability of the mutants to degrade RNA polymerase II, leading to transcription of critical genes that sustain growth.
Douville
Julie
J
Département de Biologie, Université de Sherbrooke, Quebec, Canada.
David
Jocelyn
J
Lemieux
Karine M
KM
Gaudreau
Luc
L
Ramotar
Dindial
D
D000935:Antifungal Agents / Q000494:pharmacology*; D015152:Blotting, Northern; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins; D009154:Mutation; D019696:Peptidylprolyl Isomerase; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D020123:Sirolimus / Q000494:pharmacology*
D000935:Antifungal Agents; D047908:Intracellular Signaling Peptides and Proteins; D029701:Saccharomyces cerevisiae Proteins; C502440:PPH3 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D019696:Peptidylprolyl Isomerase; C490203:RRD1 protein, S cerevisiae; D020123:Sirolimus