[Cytotoxic effect in human colon of enterohemorrhagic Escherichia coli isolated from calves with bloody diarrhea].
Shiga toxin-producing E. coli (STEC) is one of the most important emergent pathogen in foods, being its main reservoir bovine cattle. STEC can cause diarrhea, hemorrhagic colitis and hemolytic-uremic syndrome. The present work have studied the cytotoxic action in human colon of cultures of two STEC strains isolated from faeces of calves with bloody diarrhea. Colonic mucosa was mounted as a diaphragm in a Ussing chamber and incubated with the cultures of pathogenic strains. Net water flow (Jw) decreased and the short-circuit current (Isc) increased significantly (p < 0.01) compared to negative control. Tissues showed an erosion of the mucose, epithelial exfoliation, and presence of pseudo-membranes in the lumen. Mild circulatory lesions were observed in the lamina propia. A moderate neutrophils infiltration was observed in the lumen and into the epithelial cells. Colonic crypts were not disrupted. Both experimental strains caused a similar lesion on colon tissues. This is the first study that shows that cultures of STEC strains isolated from bovine cattle produce cytotoxic effects in vitro in human colon.
Pistone Creydt
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V
Depto de Fisiologia, Facultad de Medicina, UBA, Paraguay 2155, 7mo piso, 1121 Buenos Aires, Argentina.
Venzano
A
A
Vilte
D A
DA
Mercado
E C
EC
Ibarra
C
C
D000818:Animals; D001692:Biological Transport; D001834:Body Water / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003106:Colon / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D009504:Neutrophils / Q000473:pathology; D013045:Species Specificity; D014774:Virulence