Methicillin-resistant Staphylococcus aureus strains in Buenos Aires teaching hospitals: replacement of the multidrug resistant South American clone by another susceptible to rifampin, minocycline and trimethoprim-sulfamethoxazole.
The aim of this study was to characterize methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered from different infectious sites of hospitalized patients at two university hospitals. Fourteen isolates were analyzed by repetitive sequence based PCR (Rep-PCR), randomly amplified polymorphic DNA assay (RAPD-PCR), and pulsed-field gel electrophoresis (PFGE). We found that a prevalent clone of MRSA, susceptible to rifampin, minocycline, and trimethoprim/sulfamethoxazole (RIF(s), MIN(s), TMS(s)) was present in both hospitals in replacement of the multiresistant MRSA South American clone, previously described in these hospitals. The staphylococcal chromosomal cassette (SCCmec) type I element was detected in this new clone.
Gardella
N
N
Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, UBA, Junín 956 (1113) Ciudad Autónoma de Buenos Aires, Argentina.
Picasso
R
R
Predari
S C
SC
Lasala
M
M
Foccoli
M
M
Benchetrit
G
G
Famiglietti
A
A
Catalano
M
M
Mollerach
M
M
Gutkind
G
G
D000047:Academies and Institutes / Q000706:statistics & numerical data; D001118:Argentina / Q000453:epidemiology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006785:Hospitals, University / Q000706:statistics & numerical data*; D006801:Humans; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance / Q000235:genetics; D008911:Minocycline / Q000494:pharmacology; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012293:Rifampin / Q000494:pharmacology; D013020:South America / Q000453:epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology; D014504:Urban Health
D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D008911:Minocycline; D008712:Methicillin; D012293:Rifampin