title: Quantification of urinary allantoin by capillary zone electrophoresis during recombinant urate oxydase (rasburicase) therapy.
abstract: OBJECTIVE
  Rasburicase (Fasturtec) is used to prevent or treat hyperuricemia associated with chemotherapy. We developed a capillary zone electrophoresis method to measure urinary allantoin, the degradation product of uric acid by rasburicase.
  
  
  METHODS
  Electrophoresis was performed using a P/ACE 5500 system (Beckman) with a fused silica capillary tube and a UV-visible detector set at 214 nm. Urine samples from 10 patients with non-Hodgkin's lymphoma were analyzed to validate the technique.
  
  
  RESULTS
  Using a sodium tetraborate running buffer, urinary allantoin was separated from related compounds and internal standard in less than 30 min. The method was linear up to 1.25 g/L (quantification limit: 30 mg/L); precision was below 10%. The total amount of allantoin excreted in patients treated by rasburicase ranged from 1.5 g to 7.9 g/4 days.
  
  
  CONCLUSIONS
  This CZE assay is a simple, rapid and reproducible method to measure allantoin in urine. Different elimination profiles have been found in patients treated with rasburicase.
authors.affiliation: Laboratoire de Biochimie A, AP-HP, 1 Avenue Claude Vellefaux, 75010 Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Kattygnarath
authors.forename: N
authors.initials: N
authors.lastname: Mounier
authors.forename: I
authors.initials: I
authors.lastname: Madelaine-Chambrin
authors.forename: B
authors.initials: B
authors.lastname: Gourmel
authors.forename: T
authors.initials: T
authors.lastname: Le Bricon
authors.forename: C
authors.initials: C
authors.lastname: Gisselbrecht
authors.forename: P
authors.initials: P
authors.lastname: Faure
authors.forename: P
authors.initials: P
authors.lastname: Houzé
mesh_terms: D000481:Allantoin / Q000652:urine*; D015415:Biomarkers; D019075:Electrophoresis, Capillary; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy / Q000652:urine; D011994:Recombinant Proteins / Q000235:genetics / Q000627:therapeutic use*; D014503:Urate Oxidase / Q000235:genetics / Q000627:therapeutic use*
chemical_list: D015415:Biomarkers; D011994:Recombinant Proteins; C469709:rasburicase; D000481:Allantoin; D014503:Urate Oxidase