title: Synthesis of linked triple helical DNAs possessing high affinity to triple helical DNA binding protein.
abstract: The synthesis of triplexes possessing an anthraquinonyl group and composed of branched oligonucleotides (ONs) is described. Binding ability of a triplex-binding protein (MBP-LOR3(ARF)) to the triplexes was evaluated by an electrophoretic mobility shift assay (EMSA). It was found that the triplex, which has an anthraquinonecarbonyl group at the 5'-end of the third strand and is connected with the pentaerythritol linker, has greater affinity to the protein than an unmodified triplex.
authors.affiliation: Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido, Gifu 501-1193, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Shibata
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ueno
authors.forename: Koichiro
authors.initials: K
authors.lastname: Shinbo
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakanishi
authors.forename: Akira
authors.initials: A
authors.lastname: Matsuda
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kitade
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D009690:Nucleic Acid Conformation
chemical_list: D004268:DNA-Binding Proteins; C051802:triplex DNA; D004247:DNA