PET imaging of glucose metabolism in a mouse model of temporal lobe epilepsy.
Here we present the first demonstration that 2-deoxy-2[18F]fluoro-D-glucose (18FDG) and micro Positron Emission Tomography (microPET) can be used successfully to monitor regional changes in brain metabolism during acute seizure induction in C57Bl/6 mice. These longitudinal studies show a significant increase in 18FDG uptake in the hippocampus (33.2%) which correlates directly with seizure severity (R2=0.86). 18FDG microPET can potentially be used to monitor the development of TLE in mouse models from the acute phase of status epilepticus to the chronic phase of spontaneous recurrent seizures. These studies provide a foundation upon which we can begin to identify genetic contributions to the metabolic signature of TLE in mice, since many transgenics are in the C57Bl/6 background strain.
Mirrione
Martine M
MM
Graduate Program in Molecular and Cellular Pharmacology, State University of New York at Stony Brook, Stony Brook, New York 11794, USA.
Schiffer
Wynne K
WK
Siddiq
Mustafa
M
Dewey
Stephen L
SL
Tsirka
Stella E
SE
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism*; D019788:Fluorodeoxyglucose F18; D005947:Glucose / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D049268:Positron-Emission Tomography
D019788:Fluorodeoxyglucose F18; D005947:Glucose