title: Analysis of macamides in samples of Maca (Lepidium meyenii) by HPLC-UV-MS/MS.
abstract: The macamides are a distinct class of secondary metabolites that have so far been found only in Lepidium meyenii Walp. (Maca). Using HPLC-UV-MS/MS, the main macamides have been identified as n-benzylhexadecanamide, n-benzyl-(9Z)-octadecenamide, n-benzyl-(9Z, 12Z)-octadecadienamide, n-benzyl-(9Z, 12Z, 15Z)-octadecatrienamide and n-benzyloctadecanamide. The identities of n-benzyl-(9Z)-octadecenamide and n-benzyl-(9Z, 12Z)-octadecadienamide were confirmed by comparison of chromatographic and spectral properties with synthetic analogues. Total macamides have been quantified by HPLC-UV in plant material from different vendors using n-benzylhexadecanamide as an external standard. The amount of macamides in the dried plant material ranged from 0.0016 to 0.0123%.
authors.affiliation: Tom's of Maine, Kennebunk, ME 04043, USA.
authors.forename: Megan M
authors.initials: MM
authors.lastname: McCollom
authors.forename: Jacquelyn R
authors.initials: JR
authors.lastname: Villinski
authors.forename: Kerry L
authors.initials: KL
authors.lastname: McPhail
authors.forename: Lyle E
authors.initials: LE
authors.lastname: Craker
authors.forename: Stefan
authors.initials: S
authors.lastname: Gafner
mesh_terms: D000577:Amides / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D029686:Lepidium / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000577:Amides