[Study on the extraction recovery of ginsenoside Re in plasma by different solid-phase cartridges].
OBJECTIVE
To optimize the solid-phase extraction method by comparison of the extraction recovery of ginsenoside Re plasma samples.


METHODS
After extracted by different solid-phase cartridges with water, acetonitrile, and different content methanol elution, the plasma samples were analyzed on an Zorbax SB-C18 column with acetonitrile-water gradient elution. From the recovery achieved, the best solid phase cartridge was found.


RESULTS
This method consists of using 40% methanol as the wash solvent, and 80% methanol for the elution. Among the three kinds of solid-phase being tested, Waters Oasis HLB cartridge was found to be the best one.


CONCLUSIONS
The average extraction recovery of the Waters Oasis HLB cartridges was between 103%-113%, it can be used in the analysis of ginsenoside Re in plasma samples.
Mao
Xiang-Jun
XJ
China Pharmaceutical University, Nanjing 210009, China.
Zhang
Chen-Feng
CF
Sun
Di
D
Sheng
Long-Sheng
LS
Wang
Guang-Ji
GJ
Liu
Wen-Ying
WY
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
D036145:Ginsenosides; C049864:ginsenoside Re