title: Phenotype and function of myeloid dendritic cells derived from African green monkey blood monocytes.
abstract: Myeloid dendritic cells probably play an important role in the immune response against HIV and SIV, and in the enhancement of CD4+ T cell infection. Here, we have investigated phenotypic and functional features of myeloid monocyte-derived DC (MDDC) from African green monkeys (AGMs). AGMs are natural hosts of SIV and exhibit no signs of abnormal T cell activation despite high SIV plasma viremia. We identified mAbs that cross-react specifically with homologous molecules expressed on AGM DC. We adapted a protocol to derive AGM MDDC by culture in the presence of GM-CSF and IL-4. The differentiated cells possessed a typical dendritic morphology and the majority were CD11c+ DC-SIGN+. AGM MDDC displayed a high expression of typical maturation markers, such as CD83, CD86 and DC-LAMP, and moderate immunostimulatory capacity, suggesting that the cells were in a semi-mature state. Stimulation resulted in further maturation, as shown by up-regulation of CD80 and decrease of endocytosis ability. However, neither increase of HLA-DR or CD40 expression nor enhanced immunostimulatory capacity was observed. The latter was associated with a low pro-inflammatory cytokine production during mixed lymphocyte reactions and a cytokine balance in favour of IL-10 in contrast to human MDDC. This is the first characterization of AGM MDDC. The tools described here are a crucial step for future studies in vivo or in vitro on the function of myeloid DC using the AGM animal model.
authors.affiliation: Unité de Régulation des Infections Rétrovirales, Institut Pasteur, 25, rue du Docteur Roux, 75015 Paris, France.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Mortara
authors.forename: Mickaël J-Y
authors.initials: MJ
authors.lastname: Ploquin
authors.forename: Abdourahmane
authors.initials: A
authors.lastname: Faye
authors.forename: Daniel
authors.initials: D
authors.lastname: Scott-Algara
authors.forename: Bruno
authors.initials: B
authors.lastname: Vaslin
authors.forename: Cécile
authors.initials: C
authors.lastname: Butor
authors.forename: Anne
authors.initials: A
authors.lastname: Hosmalin
authors.forename: Françoise
authors.initials: F
authors.lastname: Barré-Sinoussi
authors.forename: Ousmane M
authors.initials: OM
authors.lastname: Diop
authors.forename: Michaela C
authors.initials: MC
authors.lastname: Müller-Trutwin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002469:Cell Separation; D002522:Chlorocebus aethiops / Q000097:blood* / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D004247:DNA / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D018664:Interleukin-12 / Q000096:biosynthesis / Q000235:genetics; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D023421:Models, Animal; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D022423:Myeloid Cells / Q000166:cytology / Q000276:immunology*; D010587:Phagocytosis; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma; D004247:DNA