Temporal and parental-specific expression of imprinted genes in a newly derived Chinese human embryonic stem cell line and embryoid bodies.
Although the study of imprinted genes in human development is very important, little is known about their expression and regulation in the early differentiation of human tissues due to lack of an appropriate model. In this study, a Chinese human embryonic stem (hES) cell line, SHhES1, was derived and fully characterized. Expression profiles of human imprinted genes were determined by Affymetrix Oligo micro-array in undifferentiated SHhES1 cells and SHhES1-derived embryoid bodies (EBs) at day 3, 8, 13 and 18. Thirty-two known human imprinted genes were detected in undifferentiated ES cells. Significantly, differential expression was found in nine genes at different stages of EB formation. Expression profile changes were confirmed by quantitative real-time reverse transcriptase-polymerase chain reaction in SHhES1 cells as well as in another independently derived hES cell line, HUES-7. In addition, the monoallelic expressions of four imprinted genes were examined in three different passages of undifferentiated ES cells and EBs of both hES cell lines. The monoallelic expressions of imprinted genes, H19, PEG10, NDNL1 and KCNQ1 were maintained in both undifferentiated hES cells and derived EBs. More importantly, with the availability of maternal peripheral blood lymphocyte sample, we demonstrated that the maternal expression of KCNQ1 and the paternal expression of NDNL1 and PEG10 were maintained in SHhES1 cells. These data provide the first demonstration that the parental-specific expression of imprinted genes is stable in EBs after extensive differentiation, also indicating that in vitro fertilization protocol does not disrupt the parental monoallelic expression of the imprinted genes examined.
Sun
Bo Wen
BW
Institute of Health Science, Shanghai JiaoTong University School of Medicine, Shanghai, China.
Yang
A Cong
AC
Feng
Yun
Y
Sun
Yi Juan
YJ
Zhu
Yu fei
Yf
Zhang
Yi
Y
Jiang
Hua
H
Li
Chun Liang
CL
Gao
Fu Rong
FR
Zhang
Zhi Hong
ZH
Wang
Wei Cheng
WC
Kong
Xiang Yin
XY
Jin
Gang
G
Fu
Shi Jun
SJ
Jin
Ying
Y
D000951:Antigens, Neoplasm; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line / Q000378:metabolism*; D002681:China; D004268:DNA-Binding Proteins; D004622:Embryo, Mammalian / Q000166:cytology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D051657:KCNQ1 Potassium Channel / Q000378:metabolism; D007621:Karyotyping; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000378:metabolism; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D039901:Totipotent Stem Cells / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D064267:Intrinsically Disordered Proteins
D000951:Antigens, Neoplasm; D051017:Apoptosis Regulatory Proteins; D004268:DNA-Binding Proteins; D051657:KCNQ1 Potassium Channel; D011506:Proteins; D016601:RNA-Binding Proteins; C496909:KCNQ1 protein, human; C510822:MAGEL2 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D064267:Intrinsically Disordered Proteins; C403091:Magel2 protein, mouse; C432965:PEG10 protein, human