Inhibition of Bacillus subtilis scoC expression by multicopy senS.
The Bacillus subtilis aprE gene, which encodes the extracellular alkaline protease, is regulated by many positive and negative transcriptional regulators. SenS is one such positive regulator consisting of 65 amino acids. We found that the senS gene on a multicopy plasmid, pSEN24, caused an increase in aprE expression in strains carrying the upstream region of aprE up to -340 with respect to the transcription initiation site but not in a strain carrying the region up to -299, which is within the binding site of the negative regulator ScoC (Hpr). Epistatic analysis showed that the pSEN24 effect was lost in a scoC-deleted mutant. In accordance with these results, the scoC transcription level as assayed by a scoC-lacZ fusion and Northern analysis was greatly reduced in the cells carrying pSEN24. From these results we conclude that multicopy senS enhances aprE expression by suppressing the transcription of scoC.
Kawachi
Eiji
E
Department of Marine Science, School of Marine Science and Technology, Tokai University, 3-20-1 Shimizuorido, Shizuoka 424-8610, Japan.
Abe
Sadanobu
S
Tanaka
Teruo
T
D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern; D017353:Gene Deletion; D018628:Gene Dosage; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D050939:Gene Fusion; D017930:Genes, Reporter; D026901:Membrane Transport Proteins / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
C088074:AprE protein, Bacteria; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; C442131:ScoC protein, Bacillus subtilis; D014157:Transcription Factors; C063501:senS protein, Bacillus subtilis; D001616:beta-Galactosidase