Molecular genetic alterations and gene expression profile of a malignant rhabdoid tumor of the kidney.
Malignant rhabdoid tumor of the kidney (MRTK) is a rare but highly aggressive tumor in children, and knowledge about the molecular signature of this tumor is limited. We report the molecular genetic alterations and gene expression profile of an MRTK tumor that arose in a 4-month-old Japanese girl. Fluorescence in situ hybridization and Southern blot analyses revealed a homozygous deletion of an approximately 0.29-Mb genomic region bordered by the Rgr and DDT genes in these tumor cells. This deleted region encodes SMARCB1, a candidate tumor suppressor gene for MRTK. Using a high-density oligonucleotide DNA array, we found increased expression of 25 genes, including genes involved in the cell cycle (10 genes), DNA replication (3 genes), cell growth (5 genes), and cell proliferation (5 genes), in this MRTK tumor sample, compared with a noncancerous kidney (NK) sample. On the other hand, 64 genes, including 4 genes regulating apoptosis, were found to show decreased expression in this MRTK tumor sample, compared with the NK sample. Among these alterations, we found alterations of expression of some genes, such as IGF2, MDK, TP53, and TNFSF10, in this MRTK tumor, as described previously. The molecular genetic alterations and altered pattern of gene expression found in this case may have contributed to the biological characteristics of the MRTK tumor that arose in our patient.
Nagata
Toshihito
T
Department of Advanced Medicine, Nihon University, School of Medicine, 30-1, Oyaguchikami-cho, Itabashi-ku, Tokyo 173-8610, Japan. tnagata@med.nihon-u.ac.jp
Takahashi
Yasuo
Y
Ishii
Yukimoto
Y
Asai
Satoshi
S
Sugahara-Kobayashi
Megumi
M
Nishida
Yayoi
Y
Murata
Akiko
A
Yamamori
Shunji
S
Ogawa
Yoshiyasu
Y
Nakamura
Takeshi
T
Murakami
Hitohiko
H
Nakamura
Masanori
M
Shichino
Hiroyuki
H
Chin
Motoaki
M
Sugito
Kiminobu
K
Ikeda
Taro
T
Koshinaga
Tsugumichi
T
Mugishima
Hideo
H
D017209:Apoptosis / Q000235:genetics; D015139:Blotting, Southern; D002455:Cell Division / Q000235:genetics; D049109:Cell Proliferation; D002869:Chromosome Aberrations; D002892:Chromosomes, Human, Pair 22; D004261:DNA Replication / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D018816:Genes, cdc; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D007680:Kidney Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D018335:Rhabdoid Tumor / Q000235:genetics*