title: Biosynthesis of poly-gamma-glutamic acid in plants: transient expression of poly-gamma-glutamate synthetase complex in tobacco leaves.
abstract: Transient expression of genes coding for the poly-gamma-glutamate (gammaPGA) synthetase system (pgs) was investigated in tobacco plants. Three genes of the pgs, pgsA, pgsB and pgsC, were separately placed under the control of the CaMV 35S promoter and introduced into tobacco leaves via Agrobacterium infection. Synthesized gammaPGA in plant tissues was detected immunologically with mouse anti-gammaPGA antiserum which specifically reacts with gammaPGA on a nitrocellulose membrane. Confirmation of gammaPGA biosynthesis in the transient expression analysis in tobacco tissue indicates that subunits of pgs complex were expressed and reassembled in a functional form.
authors.affiliation: Department of Biology and Geoscience, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan. ytarui@sci.osaka-cu.ac.jp
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tarui
authors.forename: Hanako
authors.initials: H
authors.lastname: Iida
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Ono
authors.forename: Wataru
authors.initials: W
authors.lastname: Miki
authors.forename: Eiji
authors.initials: E
authors.lastname: Hirasawa
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Fujita
authors.forename: Toshio
authors.initials: T
authors.lastname: Tanaka
authors.forename: Makoto
authors.initials: M
authors.lastname: Taniguchi
mesh_terms: D017343:Genes, Plant / Q000235:genetics; D005970:Glutamate Synthase / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D011099:Polyglutamic Acid / Q000096:biosynthesis* / Q000235:genetics; D012231:Rhizobium / Q000235:genetics; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics; D019076:Transgenes
chemical_list: D011099:Polyglutamic Acid; D005970:Glutamate Synthase