Inhibition of cellular proliferation by diterpenes, topoisomerase II inhibitor.
We examined the effects of 12 terpene compounds derived from the roots of Euphorbia kansui on the proliferative activity of Xenopus embryo cells. Eight of these compounds showed significant inhibition of cellular proliferation even at low concentrations, while four of them needed to be present at higher concentrations to inhibit cellular proliferation. In order to define the mechanism of inhibition of cellular proliferation by these compounds, the effects of diterpene compounds on the activity of topoisomerase II were measured. Most of the diterpene compounds that inhibited cellular proliferation also inhibited topoisomerase II activity.
Miyata
Shohei
S
Department of Chemistry, College of Humanities and Sciences, Nihon University, Sakurajosui, Tokyo 156-8550, Japan. miyata@chs.nihon-u.ac.jp
Wang
Li-Yan
LY
Yoshida
Chisato
C
Kitanaka
Susumu
S
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D004250:DNA Topoisomerases, Type II / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D028481:Euphorbia / Q000737:chemistry; D005260:Female; D008297:Male; D059005:Topoisomerase II Inhibitors; D014982:Xenopus laevis
D004224:Diterpenes; D004791:Enzyme Inhibitors; D059005:Topoisomerase II Inhibitors; D004250:DNA Topoisomerases, Type II