title: Cryptosporidium infection: diagnostic techniques.
abstract: The aim of this study was to compare a rapid immunological test and a PCR method with the conventional morphological technique for the identification of Cryptosporidium in faecal samples. Cryptosporidium was found in five samples by Kinyoun acid-fast stain. Five samples yielded positive results on immunoassay, three of which yielded negative results on microscopy. Thus, only two patients were positive for Cryptosporidium according to both methods. PCR analysis confirmed only one sample as positive. Non-homogeneous distribution of parasites in stool samples, lack of oocysts in the tested sample and antigenic diversity among Cryptosporidium species may explain the poor agreement among the three tests. Based on our experience, microscopy test with Kinyoun stain is the best and cheapest way to detect Cryptosporidium spp. in faecal samples. With this method, we have found a 5.4% prevalence of Cryptosporidium infection in our area, similar to those reported for other regions of Italy and Europe.
authors.affiliation: Clinic of Infectious Diseases, Department of Molecular Biology, University of Siena, Siena, Italy. magi@unisi.it
authors.forename: Barbara
authors.initials: B
authors.lastname: Magi
authors.forename: Valentina
authors.initials: V
authors.lastname: Canocchi
authors.forename: Giacinta
authors.initials: G
authors.lastname: Tordini
authors.forename: Carla
authors.initials: C
authors.lastname: Cellesi
authors.forename: Angela
authors.initials: A
authors.lastname: Barberi
mesh_terms: D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D002648:Child; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000469:parasitology; D003458:Cryptosporidium / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D003967:Diarrhea / Q000175:diagnosis / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008853:Microscopy / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan