Hydrogen production from glucose by anaerobes.
Various anaerobes were cultivated in media containing glucose. When 100 mL of thioglycollate medium containing 2.0% (w/v) glucose was used, Clostridium butyricum ATCC 859, NBRC 3315, and NBRC 13949 evolved 227-243 mL of biogas containing about 180 mL of hydrogen in 1 day. Although some strains had some resistance against oxygen, C. butyricum ATCC 859 and 860 did not have it. C. butyricum NBRC 3315 and Enterobacter aerogenes NBRC 13534 produced hydrogen in the presence of glucose or pyruvic acid, and E. aerogenes NBRC 13534 produced hydrogen by not only glucose and pyruvic acid but also dextrin, sucrose, maltose, galactose, fructose, mannose, and mannitol. When a medium containing 0.5% (w/v) yeast extract and 2.0% (w/v) glucose was used, E. aerogenes NBRC 13534 evolved more biogas and hydrogen than C. butyricum NBRC 3315 in the absence of reducing agent.
Ogino
Hiroyasu
H
Department of Chemical Engineering, Osaka Prefecture University, Sakai, Japan. ogino@chemeng.osakafu-u.ac.jp
Miura
Takashi
T
Ishimi
Kosaku
K
Seki
Minoru
M
Yoshida
Hiroyuki
H
D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001709:Biotechnology; D050260:Carbohydrate Metabolism; D046969:Clostridium acetobutylicum / Q000378:metabolism; D046972:Clostridium butyricum / Q000378:metabolism; D003470:Culture Media; D021902:Enterobacter aerogenes / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D019163:Reducing Agents
D003470:Culture Media; D019163:Reducing Agents; D006859:Hydrogen; D005947:Glucose