title: Adaptive cellular protection against UVA-1-induced lipid peroxidation in human dermal fibroblasts shows donor-to-donor variability and is glutathione dependent.
abstract: Photo-oxidative stress and subsequent lipid peroxidation (LPO) is one of the major mechanisms of UVA-related skin pathology. The skin's protection system against photo-oxidative stress involves low molecular scavengers as well as highly specialised antioxidant enzymes like glutathione peroxidase (GPX). Against repetitive UVA-1 exposures in vitro it is partly adaptive, as recent studies have shown exemplarily for antioxidant enzymes. We now investigated in vitro by repetitively irradiating human dermal fibroblasts with UVA-1 whether this adaptive response might reflect itself in reduced cellular membrane damage, that is, LPO. Our experiments show that the degree of cellular protection against LPO and the adaptive potential of the cells against a repetitive UVA-1 exposure varies from donor-to-donor and depends highly on glutathione.
authors.affiliation: Department of Dermatology and Allergology, University of Ulm, Maienweg 12, 89081 Ulm-Söflingen, Germany.
authors.forename: Lars Alexander
authors.initials: LA
authors.lastname: Schneider
authors.forename: Joachim
authors.initials: J
authors.lastname: Dissemond
authors.forename: Peter
authors.initials: P
authors.lastname: Brenneisen
authors.forename: Adelheid
authors.initials: A
authors.lastname: Hainzl
authors.forename: Karlis
authors.initials: K
authors.lastname: Briviba
authors.forename: Meinhard
authors.initials: M
authors.lastname: Wlaschek
authors.forename: Karin
authors.initials: K
authors.lastname: Scharffetter-Kochanek
mesh_terms: D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002478:Cells, Cultured; D003627:Data Interpretation, Statistical; D020405:Dermis / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D005347:Fibroblasts / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D005978:Glutathione / Q000032:analysis / Q000502:physiology*; D005979:Glutathione Peroxidase / Q000502:physiology; D006801:Humans; D015227:Lipid Peroxidation / Q000528:radiation effects*; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000032:analysis; D012879:Skin Physiological Phenomena; D014466:Ultraviolet Rays
chemical_list: D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D019328:Buthionine Sulfoximine; D005979:Glutathione Peroxidase; D005978:Glutathione