title: Cytokine expression in porcine lungs experimentally infected with Mycoplasma hyopneumoniae.
abstract: To gain further insight into the pathogenesis of porcine enzootic pneumonia (PEP), cytokine expression in different pulmonary compartments was examined. Mycoplasma hyopneumoniae (Mh) and proinflammatory and immunoregulatory cytokines (IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10 and TNF-alpha) were detected by immunohistochemical methods in porcine lungs experimentally infected with Mh. Ten pigs were inoculated intranasally with Mh and killed in pairs weekly from 1- to 5-week post-inoculation (wpi). Three Mh-free pigs were taken as controls. Mh-antigen was shown in paraffin-wax-embedded tissues by immunohistochemistry in the luminal surface of bronchial and bronchiolar epithelial cells of all Mh-infected pigs. Significant increase in cytokine expression was detected on snap-frozen tissues from the bronchoalveolar exudate of the airways, mononuclear cells of the alveolar septa and macrophages and lymphocytes of the peribronchial and peribronchiolar lymphoid tissue, from 1 wpi onwards, compared to expression in non-pneumonic lungs. The main cytokines in the BALT of Mh-infected animals that showed an increase were IL-2, IL-4, IL-8, IL-10 and TNF-alpha. In the alveolar septa and bronchoalveolar exudate IL-1 (alpha and beta), IL-2, IL-4, IL-8 and IL-10 expression also increased in infected animals.
authors.affiliation: Department of Comparative Pathology, Veterinary Faculty, University of Las Palmas de Gran Canaria, Trasmontaña s/n, 35416 Arucas, Gran Canaria, Spain.
authors.forename: Héctor
authors.initials: H
authors.lastname: Lorenzo
authors.forename: Oscar
authors.initials: O
authors.lastname: Quesada
authors.forename: Patricia
authors.initials: P
authors.lastname: Assunçao
authors.forename: Ayoze
authors.initials: A
authors.lastname: Castro
authors.forename: Francisco
authors.initials: F
authors.lastname: Rodríguez
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001831:Body Temperature; D001980:Bronchi / Q000276:immunology / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006801:Humans; D007150:Immunohistochemistry / Q000662:veterinary; D007378:Interleukins / Q000096:biosynthesis* / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology / Q000382:microbiology; D008297:Male; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology* / Q000382:microbiology; D011897:Random Allocation; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha