Analysis of antibiotics in urine and wipe samples from environmental and biological monitoring--comparison of HPLC with UV-, single MS- and tandem MS-detection.
Results of the simultaneous determination of the structurally different antibiotics cefazoline, cefotiame, cefuroxime, chloramphenicol, ciprofloxacin, ofloxacin, sulfamethoxazole and trimethoprim from environmental and biological monitoring using high-performance liquid chromatography with UV, single mass and tandem mass spectrometry were compared. For sample enrichment and clean-up a SPE method using bakerbond C18 cartridges was developed. Mean recovery rates were above 70%. Because of the complex urine matrix, only the wipe samples could be analyzed by UV-detection. However, UV-detection and single MS-detection are useful for control measurements after spillage, e.g. (LOD=1-2 ng/cm(2)). Samples from biological monitoring of occupational uptake should be analyzed by LC-MS/MS. The limits of detection (LOD) in urine ranged from 0.4 to 70 microg/L for LC-MS and 0.01 to 0.9 microg/L for LC-MS/MS detection. The limits of detection in wipe samples ranged from 0.003 to 0.13 ng/cm(2).
Tuerk
Jochen
J
Institute of Energy and Environmental Technology (IUTA), Bliersheimer Strasse 60, D-47229 Duisburg, Germany. tuerk@iuta.de
Reinders
Marius
M
Dreyer
Dennis
D
Kiffmeyer
Thekla K
TK
Schmidt
Klaus Gerhard
KG
Kuss
Heinz-Martin
HM
D000900:Anti-Bacterial Agents / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004781:Environmental Exposure / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents