[Analysis of chloramphenicol in shrimps by gas chromatography tandem mass spectrometry].
OBJECTIVE
A sensitive method based on isotope dilution gas chromatography tandem mass spectrometry is described for the determination of the antibiotic chloramphenicol (Cap) in shrimp tissue.


METHODS
Homogenized shrimp samples spiked with an isotopically labeled internal standard (d5-Cap) were extracted with ethyl acetate extraction, defatted with hexane, followed by a clean-up step on a SPE C18 cartridge, and then the elute was evaporated and derivatized with Sylon BFT {N,O-bis (trimethylsily) trifluoroacetamide[BSTFA]-trimethylchlorosilane [TMCS] ,99 + 1}. Analyzes were determined by GC/MS-MS in MRM mode.


RESULTS
Internal calibration curves obtained for Cap/ d5-Cap in the 5 to 500 pg range were linear with correlation coefficients of 0.9958. Limit of quantitation (LOQ) was 0.3ng/g. Shrimp tissues were fortified with Cap at 0.3, 0.5, 1.0 ng/g level, average recoveries were 104%, 111% and 95% , respectively.


CONCLUSIONS
The proposed method allows the detection, quantitation and confirmation of Cap in shrimps with high accuracy and sensitivity.
Yang
Xin
X
National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhou
Rui
R
Zhao
Yun-Feng
YF
Wu
Yong-Ning
YN
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002701:Chloramphenicol / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007554:Isotopes; D033561:Penaeidae / Q000737:chemistry; D012680:Sensitivity and Specificity; D012758:Shellfish / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000900:Anti-Bacterial Agents; D007554:Isotopes; D002701:Chloramphenicol