title: [A study on stem cell mobilization after partial liver transplantation in rats].
abstract: OBJECTIVE
  To explore the role of stem cell mobilization on regeneration of partially grafted livers.
  
  
  METHODS
  Rats models with cross-sex 50% PLTx (partial liver transplantation) were established. The rats were divided into three groups: PLTx, WLTx (whole liver transplantation) and sham operation groups. Bone marrow and liver samples were collected on days 1, 3, 5, 7 postoperatively (each n = 6). The quantitative variations of the cells with stem cell markers in the bone marrow, including beta2m-/Thy-1.1+, CD45+/CD34+, Flt2/3+ and c-kit+ markers, were detected using flow cytometry. Sry gene positive cells in donor livers were detected by fluorescent in situ hybridization (FISH), and the expressions of CD34, c-kit and Thy-1.1 were detected by immunohistochemistry technique.
  
  
  RESULTS
  Compared with the WLTx and sham operation groups, beta2m-/Thy-1.1+, CD45+/CD34+ cells in bone marrows in the PLTx group increased on the first postoperative day and decreased on the following days. The CD34, c-kit and Thy-1.1 positive cells detected in portal tract areas peaked during the 3-5 postoperative days. CD34+/CD45+ positive cells could be detected. The expressions of CD34, c-kit and Thy-1.1 positive cells were rare in the WLTx and sham operation groups. Sry+ cells could be detected in portal tract areas and few Sry+/CD34+ and Sry+/Thy-1.1+cells were detected.
  
  
  CONCLUSIONS
  In the PLTx group, the stem cells in the bone marrow were mobilized and stem cells in the liver were activated.
authors.affiliation: Institute of Hepatology, People's Hospital, Peking University, Beijing 100044, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Lai
authors.initials: L
authors.lastname: Wei
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Chen
authors.forename: Xiao-ben
authors.initials: XB
authors.lastname: Pan
authors.forename: Xu
authors.initials: X
authors.lastname: Cong
authors.forename: Ran
authors.initials: R
authors.lastname: Fei
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D017493:Leukocyte Common Antigens / Q000276:immunology; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology; D018800:Thy-1 Antigens / Q000276:immunology
chemical_list: D018952:Antigens, CD34; D018800:Thy-1 Antigens; D017493:Leukocyte Common Antigens