title: Ultra-sensitive immunoassays using multi-photon-detection in diagnostic proteomics of blood.
abstract: Multiphoton-detection methods that detect as little as 1000 atoms of (125)I-streptavidin increase the sensitivity of immunoassays by 200- to 1000-fold (1 femtogram/mL). Improved background suppression allows 20- to 100-fold improvements in sensitivity for conventional immunoassays (10-50 femtogram/mL). Quantitation of low abundance biomarkers in blood (PSA, TNFalpha, VEGF, IL-1beta, IL-6, and IL-8), for the first time for complete patient cohorts, indicates that very high analytical sensitivity and new statistical methods are crucial for serum-based diagnostic proteomics.
authors.affiliation: BioTraces Inc., Herndon, Virginia, USA.
authors.forename: Andrzej K
authors.initials: AK
authors.lastname: Drukier
authors.forename: Natascha
authors.initials: N
authors.lastname: Ossetrova
authors.forename: Elena
authors.initials: E
authors.lastname: Schors
authors.forename: Larry R
authors.initials: LR
authors.lastname: Brown
authors.forename: John
authors.initials: J
authors.lastname: Tomaszewski
authors.forename: Richard
authors.initials: R
authors.lastname: Sainsbury
authors.forename: Jasminka
authors.initials: J
authors.lastname: Godovac-Zimmermann
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001331:Automation; D015415:Biomarkers / Q000378:metabolism; D019921:Biotinylation; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry*; D001943:Breast Neoplasms / Q000097:blood / Q000378:metabolism; D016022:Case-Control Studies; D015331:Cohort Studies; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015497:HIV-1 / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007375:Interleukin-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D008875:Middle Aged; D017785:Photons; D017430:Prostate-Specific Antigen / Q000097:blood; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D020543:Proteome; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D017430:Prostate-Specific Antigen