Layer-by-layer assembly of nacre-like nanostructured composites with antimicrobial properties.
In a recent report, we have presented the layer-by-layer (LBL) assembly of a biomimetic nanostructured composite from Na(+)-montmorillonite clay nanosheets and poly(diallylmethylammonium chloride) (Tang, Z.; Kotov, N.; Magonov, S.; Ozturk, B. Nat. Mater. 2003, 2, 413). The structure, deformation mechanism, and mechanical properties of the material are very similar to those of natural nacre and lamellar bones. This fact prompts further investigation of these composites as potential bone implants. LBL assembly affords preparation of multifunctional composites, and here we demonstrate that not only mechanical strength, but also antibacterial activity, can be introduced in these implantable materials by alternating clay layers with starch-stabilized silver nanoparticles. The resulting composite showed excellent structural stability with no detectable levels of silver lost over a 1 month period. Evaluation of the antibacterial properties showed almost complete growth inhibition of E. coli over an 18 h period. The amount of silver eluted from the LBL composite over a 1 month period was determined to be only 0.5-3.0 microg/L. This concentration of silver did not prevent the growth of the mammalian tissue cultures. The LBL composite has shown biocompatibility with the human osteoblast cell line.
Podsiadlo
Paul
P
Department of Chemical Engineering, The University of Michigan, Ann Arbor, 48109, USA.
Paternel
Stephen
S
Rouillard
Jean-Marie
JM
Zhang
Zhengfei
Z
Lee
Jaebeom
J
Lee
Jung-Woo
JW
Gulari
Erdogan
E
Kotov
Nicholas A
NA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry; D000890:Anti-Infective Agents; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D060734:Nacre; D049329:Nanostructures / Q000737:chemistry; D012834:Silver; D013499:Surface Properties
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D060734:Nacre; D012834:Silver