CART (cocaine- and amphetamine-regulated transcript) peptide receptors: specific binding in AtT20 cells.
Given previous evidence for CART (cocaine- and amphetamine-regulated transcript) signaling in AtT20 cells, the binding of [125I]-CART61-102 was characterized in these cells. The binding was specific, saturable, dependent on time, pH, temperature and protein concentration, with a Bmax of 101.4+/-8.8 fmol/mg protein and a Kd of 21.9+/-8.0 pM. Only active CART55-102, but not other peptides or drugs, inhibited the [125I]-CART61-102 binding. These data are the first demonstration of specific receptor binding for CART peptides.
Vicentic
Aleksandra
A
Neuroscience Division, Yerkes National Primate Research Center of Emory University, 954 North Gatewood Road, Atlanta Georgia 30033, USA. avicen2@emory.edu
Lakatos
Anita
A
Kuhar
Michael J
MJ
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013696:Temperature; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; C428723:cocaine- and amphetamine-regulated transcript protein (55-102)