Two domains are critical for the nuclear localization of soluble adenylyl cyclase.
Soluble adenylyl cyclase (sAC) is a newly identified source of cyclic adenosine 3',5'-monophosphate (cAMP). Unlike the well-known transmembrane adenylyl cyclases (tmACs), sAC locates to the nucleus, mitochondria and microtubules. For most cAMP-signaling microdomains, there is always an AC nearby, for example tmAC. But it was until the discovery of sAC that there was not known cAMP resource in the nucleus. sAC associates with nuclear cAMP-signaling microdomains, which were once considered to depend on the diffusion of cAMP produced by tmAC. In this report, we focus on the truncated soluble adenylyl cyclase (tsAC), the most common existence form of sAC in tissues. Two domains (145-200 aa and 257-318 aa) related with sAC nuclear localization were present here. The findings provide evidence that these two domains are critical for the nuclear localization of sAC and they collocated with the catalytic domains.
Feng
Qiping
Q
Department of Biochemistry and Molecular Biology, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5# Dong Dan San Tiao, Beijing 100005, China.
Zhang
Yongwei
Y
Li
Yunfeng
Y
Liu
Zhuo
Z
Zuo
Jin
J
Fang
Fude
F
D000262:Adenylyl Cyclases / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D020134:Catalytic Domain; D002467:Cell Nucleus / Q000201:enzymology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D009154:Mutation; D017434:Protein Structure, Tertiary; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D013737:Testis / Q000166:cytology / Q000378:metabolism; D014162:Transfection
D018076:DNA, Complementary; D000262:Adenylyl Cyclases