Concentration-dependent suppressive effect of shrimp head protein hydrolysate on dehydration-induced denaturation of lizardfish myofibrils.
To utilize fishery waste products as functional food material, the shrimp head protein hydrolysate (SHPH) was produced from three species of shrimp wastes, Northern pink shrimp, Endeavour shrimp and black tiger shrimp, by enzymatic hydrolysis. The SHPH was used as a natural food preservative by adding to lizardfish myofibrils at concentrations ranging from 2.5% to 10%. Their effects on the state of water and the denaturation of myofibrils during dehydration were evaluated. The amount of monolayer and multilayer water in myofibrils containing SHPH were higher than those without SHPH (control). DSC analyses revealed that the amount of unfrozen water increased significantly after addition of SHPH. The Ca-ATPase inactivation rate of myofibrils containing SHPH decreased during dehydration while 5-7.5% concentrations of SHPH exhibited optimum effect regardless of the species. The results implicated that SHPH can be used as an alternative food preservative for suppressive the dehydration-induced denaturation of myofibrils.
Ruttanapornvareesakul
Yaowalux
Y
Graduate School of Science and Technology, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8521, Japan.
Ikeda
Misako
M
Hara
Kenji
K
Osatomi
Kiyoshi
K
Osako
Kazufumi
K
Kongpun
Orawan
O
Nozaki
Yukinori
Y
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005399:Fishes / Q000378:metabolism*; D005519:Food Preservation / Q000379:methods*; D009210:Myofibrils / Q000378:metabolism*; D033561:Penaeidae / Q000737:chemistry*; D011489:Protein Denaturation / Q000187:drug effects*; D011492:Protein Hydrolysates / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D020539:Sequence Analysis, Protein; D014867:Water / Q000737:chemistry
D011492:Protein Hydrolysates; D014867:Water; D000252:Calcium-Transporting ATPases