Generation of large numbers of transgenic Kentucky bluegrass (Poa pratensis L.) plants following biolistic gene transfer.
A very efficient transformation system, using biolistic bombardment, has been developed for the production of transgenic plants of Kentucky bluegrass (Poa pratensis L.). Embryogenic calli, initiated from immature embryos, were transformed either with pAct1IHPT-4 containing the hygromycin phosphotransferase (hpt) gene or with pDM803 containing the phosphinothricin acetyltransferase (bar) gene and the beta-glucuronidase (uidA) gene. In total 119 independent transgenic plants were recovered from 153 hygromycin-resistant lines. Bialaphos selection yielded a total of 99 bialaphos-resistant lines and from these 34 independent transgenic plants were recovered. Southern blot analysis demonstrated the independent nature of the transgenic plants and also revealed a complex transgene integration pattern with multiple insertions.
Gao
Caixia
C
DLF-Trifolium A/S, Research Division, Hoejerupvej 31, 4660 Store Heddinge, Denmark. cxg@dlf.dk
Jiang
Li
L
Folling
Marianne
M
Han
Liebao
L
Nielsen
Klaus Kristian
KK
D019470:Biolistics / Q000379:methods*; D005819:Genetic Markers; D005966:Glucuronidase / Q000235:genetics; D007629:Kentucky; D009943:Organophosphorus Compounds / Q000494:pharmacology; D030821:Plants, Genetically Modified; D031785:Poa / Q000033:anatomy & histology / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D012038:Regeneration; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic; D019076:Transgenes / Q000235:genetics
D005819:Genetic Markers; D009943:Organophosphorus Compounds; C003122:bialaphos; D005966:Glucuronidase