title: Different properties of SV channels in root vacuoles from near isogenic Al-tolerant and Al-sensitive wheat cultivars.
abstract: Patch-clamp experiments revealed that near isogenic ET8 (Al-tolerant) and ES8 (Al-sensitive) wheat cultivars differed significantly in slow vacuolar channel properties. Under control conditions, whole vacuole currents displayed faster deactivation in ES8. Application of 1.4 microM vacuolar Al3+ caused a 20 mV increase in the activation threshold and slowed activation kinetics in ET8 but not in ES8. Channel density was about 30% higher in ES8 than ET8, and was not altered by 24 h aluminium pre-treatment. However, the activation threshold was reduced in Al-pre-treated ES8. Overall, our data suggests that Alt1 locus may control more than the plasma membrane malate channel in wheat.
authors.affiliation: School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tas 7001, Australia.
authors.forename: Tim
authors.initials: T
authors.lastname: Wherrett
authors.forename: Sergey
authors.initials: S
authors.lastname: Shabala
authors.forename: Igor
authors.initials: I
authors.lastname: Pottosin
mesh_terms: D000535:Aluminum / Q000378:metabolism / Q000494:pharmacology*; D007473:Ion Channels / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008293:Malates / Q000378:metabolism; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors; D014908:Triticum / Q000235:genetics* / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D007473:Ion Channels; D008293:Malates; D000535:Aluminum