title: Cell volume increase in murine MC3T3-E1 pre-osteoblasts attaching onto biocompatible tantalum observed by magnetic AC mode atomic force microscopy.
abstract: Magnetic AC mode (MACmode) atomic force microscopy (AFM) was used to study murine (mouse) MC3T3-E1 preosteoblastic cells attached to biocompatible tantalum substrates. Cell volumes of attached cells derived from AFM images were compared to volumes of detached cells in suspension measured by the Coulter sizing technique. An increase of approximately 50% in cell volume was observed when the cells attached to planar tantalum substrates and developed a flattened structure including lamellipodia. We address thoroughly the issues general to the AFM determination of absolute cell volumes, and compare our magnetic AC mode AFM measurements to hitherto reported cell volume determinations by contact mode AFM.
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Department of Physics and Astronomy, University of Aarhus, DK-8000 Aarhus, Denmark. klembt@phys.au.dk
authors.forename: L Klembt
authors.initials: LK
authors.lastname: Andersen
authors.forename: S Antoranz
authors.initials: SA
authors.lastname: Contera
authors.forename: J
authors.initials: J
authors.lastname: Justesen
authors.forename: M
authors.initials: M
authors.lastname: Duch
authors.forename: O
authors.initials: O
authors.lastname: Hansen
authors.forename: J
authors.initials: J
authors.lastname: Chevallier
authors.forename: M
authors.initials: M
authors.lastname: Foss
authors.forename: F S
authors.initials: FS
authors.lastname: Pedersen
authors.forename: F
authors.initials: F
authors.lastname: Besenbacher
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D048429:Cell Size; D002478:Cells, Cultured; D008280:Magnetics; D051379:Mice; D018625:Microscopy, Atomic Force / Q000379:methods*; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure*; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D013635:Tantalum / Q000378:metabolism*; D016707:Tissue Fixation
chemical_list: D001672:Biocompatible Materials; D013635:Tantalum