The garlic ingredient diallyl sulfide inhibits cytochrome P450 2E1 dependent bioactivation of acrylamide to glycidamide.
Genotoxic effects of acrylamide are supposed to result from oxidative biotransformation to glycidamide. After incubation of rat liver slices with acrylamide we detected free glycidamide using a liquid chromatography tandem mass spectrometric method. Glycidamide formation was diminished in the presence of the cytochrome P450 2E1 inhibitor diallyl sulfide (DAS), which is a specific ingredient of garlic. This may be relevant to human health since the suggested carcinogenic risk of dietary acrylamide may be reduced by concomitant intake of garlic.
Taubert
Dirk
D
Department of Pharmacology, University Hospital of Cologne, Gleueler Str. 24, D-50931 Cologne, Germany. dirk.taubert@medizin.uni-koeln.de
Glöckner
Reinhild
R
Müller
Dieter
D
Schömig
Edgar
E
D020106:Acrylamide / Q000378:metabolism* / Q000493:pharmacokinetics; D000498:Allyl Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001711:Biotransformation / Q000187:drug effects; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism* / Q000493:pharmacokinetics; D005737:Garlic / Q000737:chemistry*; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013440:Sulfides / Q000302:isolation & purification / Q000494:pharmacology*
D000498:Allyl Compounds; D004852:Epoxy Compounds; D013440:Sulfides; D020106:Acrylamide; C038491:allyl sulfide; C071834:glycidamide; D019392:Cytochrome P-450 CYP2E1