Aerobic phenanthrene biodegradation in a two-phase partitioning bioreactor.
The aerobic degradation of phenanthrene by a Pseudomonas migulae strain under classical mechanical aeration and under photosynthetic oxygenation (using a Chlorella sorokiniana strain) in a two-phase partitioning bioreactor (TPPB) constructed with silicone oil as organic phase was investigated. When traditional mechanical aeration was used, an increase in the aeration and/or in the agitation rate enhanced phenanthrene biodegradation. Thus, phenanthrene removal rates (based on the total liquid volume of cultivation) ranged from 22 +/- 1 to 36 +/- 2 mg/l h at 100 rpm and 1 vvm and 400 rpm and 3 vvm, respectively. On the other hand, during phenanthrene biodegradation using the algal-bacterial microcosm a maximum rate of 8.1 +/- 1.2 mg/l h at 200 rpm and 8000 lux of illuminance was achieved.
Muñoz
R
R
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Sweden.
Rolvering
C
C
Guieysse
B
B
Mattiasson
B
B
D000704:Analysis of Variance; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002708:Chlorella / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D010100:Oxygen / Q000378:metabolism; D010616:Phenanthrenes / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
D010616:Phenanthrenes; D014874:Water Pollutants, Chemical; C031181:phenanthrene; D010100:Oxygen