Immobilization induces alterations in the outer membrane protein pattern of Yersinia ruckeri.
We compared the outer membrane protein (OMP) pattern of 2-day-old immobilized Yersinia ruckericells (IC) with that of early (FC24) and late (FC48) stationary-phase planktonic counterparts. Fifty-five OMPs were identified. Principal component analysis discriminated between the protein maps of FC and IC. Some OMPs involved in bacterial adaptation were accumulated by both FC48 and IC but the expression of other proteins was controlled by the sessile mode of growth.
Coquet
Laurent
L
IBBR Group, Laboratory Polymères, Biopolymères, Membranes, UMR 6522 CNRS, University of Rouen, France.
Cosette
Pascal
P
Dé
Emmanuelle
E
Galas
Ludovic
L
Vaudry
Hubert
H
Rihouey
Christophe
C
Lerouge
Patrice
P
Junter
Guy-Alain
GA
Jouenne
Thierry
T
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D018441:Biofilms; D018929:Cell Culture Techniques / Q000379:methods; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013058:Mass Spectrometry; D010457:Peptidoglycan; D010933:Plankton / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D025341:Principal Component Analysis; D017434:Protein Structure, Tertiary; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D016622:Silver Staining; D013997:Time Factors; D015007:Yersinia / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D010457:Peptidoglycan; D011108:Polymers; D020543:Proteome