title: Temporal and parental-specific expression of imprinted genes in a newly derived Chinese human embryonic stem cell line and embryoid bodies.
abstract: Although the study of imprinted genes in human development is very important, little is known about their expression and regulation in the early differentiation of human tissues due to lack of an appropriate model. In this study, a Chinese human embryonic stem (hES) cell line, SHhES1, was derived and fully characterized. Expression profiles of human imprinted genes were determined by Affymetrix Oligo micro-array in undifferentiated SHhES1 cells and SHhES1-derived embryoid bodies (EBs) at day 3, 8, 13 and 18. Thirty-two known human imprinted genes were detected in undifferentiated ES cells. Significantly, differential expression was found in nine genes at different stages of EB formation. Expression profile changes were confirmed by quantitative real-time reverse transcriptase-polymerase chain reaction in SHhES1 cells as well as in another independently derived hES cell line, HUES-7. In addition, the monoallelic expressions of four imprinted genes were examined in three different passages of undifferentiated ES cells and EBs of both hES cell lines. The monoallelic expressions of imprinted genes, H19, PEG10, NDNL1 and KCNQ1 were maintained in both undifferentiated hES cells and derived EBs. More importantly, with the availability of maternal peripheral blood lymphocyte sample, we demonstrated that the maternal expression of KCNQ1 and the paternal expression of NDNL1 and PEG10 were maintained in SHhES1 cells. These data provide the first demonstration that the parental-specific expression of imprinted genes is stable in EBs after extensive differentiation, also indicating that in vitro fertilization protocol does not disrupt the parental monoallelic expression of the imprinted genes examined.
authors.affiliation: Institute of Health Science, Shanghai JiaoTong University School of Medicine, Shanghai, China.
authors.forename: Bo Wen
authors.initials: BW
authors.lastname: Sun
authors.forename: A Cong
authors.initials: AC
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.forename: Yi Juan
authors.initials: YJ
authors.lastname: Sun
authors.forename: Yu fei
authors.initials: Yf
authors.lastname: Zhu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Chun Liang
authors.initials: CL
authors.lastname: Li
authors.forename: Fu Rong
authors.initials: FR
authors.lastname: Gao
authors.forename: Zhi Hong
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Wei Cheng
authors.initials: WC
authors.lastname: Wang
authors.forename: Xiang Yin
authors.initials: XY
authors.lastname: Kong
authors.forename: Gang
authors.initials: G
authors.lastname: Jin
authors.forename: Shi Jun
authors.initials: SJ
authors.lastname: Fu
authors.forename: Ying
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000951:Antigens, Neoplasm; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line / Q000378:metabolism*; D002681:China; D004268:DNA-Binding Proteins; D004622:Embryo, Mammalian / Q000166:cytology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D051657:KCNQ1 Potassium Channel / Q000378:metabolism; D007621:Karyotyping; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000378:metabolism; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D039901:Totipotent Stem Cells / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D064267:Intrinsically Disordered Proteins
chemical_list: D000951:Antigens, Neoplasm; D051017:Apoptosis Regulatory Proteins; D004268:DNA-Binding Proteins; D051657:KCNQ1 Potassium Channel; D011506:Proteins; D016601:RNA-Binding Proteins; C496909:KCNQ1 protein, human; C510822:MAGEL2 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D064267:Intrinsically Disordered Proteins; C403091:Magel2 protein, mouse; C432965:PEG10 protein, human