[Telomerase activity and telomere length in CD4+,CD8+ and CD19+ lymphocytes from patients with systemic lupus erythematosus].
To investigate the telomerase activity and the telomere length in CD4(+), CD8(+) and CD19(+) lymphocytes from patients with systemic lupus erythematosus (SLE).



Telomerase activity of CD4(+), CD8(+) and CD19(+) cells from patients with SLE and normal controls was examined by telomeric repeats amplification protocol. Telomere length in those cells was measured by Southern blot method.



CD4(+), CD8(+) and CD19(+) cells in patients with SLE showed high telomerase activity, but only telomerase activity of CD19(+) cells was significantly higher than that in controls. The length of telomere in CD4(+) and CD8(+) cells was significantly shorter than that in controls, but not in CD19(+) cells.



Higher telomerase activity in CD19(+) cells and shortened telomere length in CD4(+) and CD8(+) cells of patients with SLE may be associated with pathogenesis of SLE.
Lin
Jin
J
Department of Hematology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Xie
Jue
J
Qian
Wen-bin
WB
D000293:Adolescent; D000328:Adult; D018941:Antigens, CD19 / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000201:enzymology*; D018414:CD8-Positive T-Lymphocytes / Q000201:enzymology*; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000201:enzymology / Q000276:immunology*; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000235:genetics*
D018941:Antigens, CD19; D019098:Telomerase