Column-switching high-performance liquid chromatographic method for the determination of zaltoprofen in rat plasma.
A direct injection column-switching high-performance liquid chromatography (HPLC) method was developed and validated for quantification of zaltoprofen in rat plasma. Following dilution with mobile phase A, i.e. acetonitrile-10mM potassium phosphate buffer (pH 6.8) (12:88, v/v) samples were directly injected to the pre-column without sample pre-purification step. After endogenous plasma components were eluted to waste, the system was switched and the analyte was eluted to the trap column. Zaltoprofen was then back-flushed to the analytical column for separation with mobile phase B, i.e. acetonitrile-10mM potassium phosphate buffer (pH 6.8) (35:65, v/v) and quantification with an ultraviolet detector at 230 nm. The calibration curve was linear in the concentration range of 40-5000 ngmL(-1). This method has been fully validated and shown to be specific, accurate and precise. The method is simple, rapid and the sample preparation is minimal and appears to be useful for the pharmacokinetic study of zaltoprofen.
Choi
Sun Ok
SO
Division of Biopharmaceutics, Department of Pharmacology, National Institute of Toxicological Research, Korea Food and Drug Administration, Nokbun-dong 5, Eunpyung-Ku, Seoul, South Korea. sochoi@kfda.go.kr
Um
So Young
SY
Jung
Sung Hee
SH
Jung
Seo Jeong
SJ
Kim
Joo Il
JI
Lee
Hwa Jeong
HJ
Chung
Soo Youn
SY
D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000097:blood; D001578:Benzopyrans / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004355:Drug Stability; D008297:Male; D011422:Propionates / Q000008:administration & dosage / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001578:Benzopyrans; D011422:Propionates; C043940:pyranoprofen