Novel primers reveal wider diversity among marine aerobic anoxygenic phototrophs.
Aerobic anoxygenic phototrophic bacteria (AAnPs) were previously proposed to account for up to 11% of marine bacterioplankton and to potentially have great ecological importance in the world's oceans. Our data show that previously used primers based on the M subunit of anoxygenic photosynthetic reaction center genes (pufM) do not comprehensively identify the diversity of AAnPs in the ocean. We have designed and tested a new set of pufM-specific primers and revealed several new AAnP variants in environmental DNA samples and genomic libraries.
Yutin
Natalya
N
Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Suzuki
Marcelino T
MT
Béjà
Oded
O
D000332:Aerobiosis; D000693:Anaerobiosis; D001483:Base Sequence; D044822:Biodiversity; D017931:DNA Primers; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010788:Photosynthesis; D010802:Phylogeny; D016992:Rhodobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*
D017931:DNA Primers