title: Physicochemical characterization of the Strychnos alkaloids by capillary zone electrophoresis.
abstract: A capillary zone electrophoresis (CZE) method has been developed for investigating the physicochemical characteristics of five Strychnos alkaloids in Strychnos nux-vomica L. Firstly, the dissociation constants of the five Strychnos alkaloids were determined, based on the relation between the effective mobility of the solutes and the buffer pH. The mathematical relationship was strictly deduced from the fundamental electrophoretic theory and the dissociation equilibrium. Secondly, an equation describing the relation between the migration time of alkaloids of similar structure and their molecular weights was developed and used to predict the migration order and to calculate the electrosomotic velocity. The results predicted by the theory agreed with those from experiments.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, China.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Fu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Xiaozhe
authors.initials: X
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.forename: Hongbin
authors.initials: H
authors.lastname: Xiao
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D008970:Molecular Weight; D011807:Quinolizines; D029909:Sophora / Q000737:chemistry; D013331:Strychnine / Q000031:analogs & derivatives / Q000737:chemistry; D031461:Strychnos nux-vomica / Q000737:chemistry*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D011807:Quinolizines; C438914:icajine; C083806:brucine; D013331:Strychnine; D000093842:Matrines