[Interleukin 10 gene -627 polymorphisms in Chinese patients with early-onset coronary heart disease].
OBJECTIVE
To investigate the relationship between interleukin 10 (IL10) gene -627 polymorphisms and serum IL10 level and early-onset coronary heart disease (CHD).


METHODS
The genotype and allele frequency of IL10 gene -627 site was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). DNA samples were obtained from 163 patients with CHD and 112 controls. Serum IL10 level was detected by ELISA.


RESULTS
No significant difference was found in the distribution of IL10 genotype and allele frequency between the healthy controls and the patients with CHD; Chi-square values were 1.9324 and 1.5703 respectively, P > 0.05. Stratification analyses based on different sex still found no significant difference in the distribution of IL10 genotype and allele frequency between the healthy controls and the CHD patients; the Chi-square values in male groups were 1.2708 versus 0.8595, and in female groups were 0.8254 versus 0.7127, P > 0.05. Serum IL10 level showed significant differences among AA genotype, AC genotype and CC genotype, but no significant difference was noted between healthy controls and CHD patients.


CONCLUSIONS
These results suggest that IL10 gene -627 polymorphisms are not associated with an increased risk of CHD, but it might assume a role in IL10 gene expression.
Wang
Yun-ying
YY
Laboratory of Marine Genetics and Breeding, Division of Life Science and Biotechnology, Ocean University of China, Qingdao, Shandong, P. R. China. sdqdwyy@126.com
Yu
Xin-juan
XJ
Wang
Pei-lin
PL
Bao
Zhen-min
ZM
D000328:Adult; D017668:Age of Onset; D044466:Asian People / Q000235:genetics; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D003327:Coronary Disease / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D016753:Interleukin-10