Toscana virus in Spain.
Toscana virus (TOSV, Phlebovirus, family Bunyaviridae) infection is one of the most prevalent arboviruses in Spain. Within the objectives of a multidisciplinary network, a study on the epidemiology of TOSV was conducted in Granada, in southern Spain. The overall seroprevalence rate was 24.9%, significantly increasing with age. TOSV was detected in 3 of 103 sandfly pools by viral culture or reverse transcription-polymerase chain reaction from a region of the L gene. Nucleotide sequence homology was 99%-100% in TOSV from vectors and patients and 80%-81% compared to the Italian strain ISS Phl.3. Sequencing of the N gene of TOSV isolates from patients and vectors indicated 87%-88% and 100% homology at the nucleotide and amino acid levels, respectively, compared to the Italian strain. These findings demonstrate the circulation of at least 2 different lineages of TOSV in the Mediterranean basin, the Italian lineage and the Spanish lineage.
Sanbonmatsu-Gámez
Sara
S
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avda. Fuerzas Armadas, s/n 18014-Granada, Spain.
Pérez-Ruiz
Mercedes
M
Collao
Ximena
X
Sánchez-Seco
María Paz
MP
Morillas-Márquez
Francisco
F
de la Rosa-Fraile
Manuel
M
Navarro-Mari
José Maria
JM
Tenorio
Antonio
A
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007303:Insect Vectors / Q000821:virology*; D008297:Male; D010691:Phlebotomus / Q000821:virology*; D010217:Phlebotomus Fever / Q000453:epidemiology* / Q000821:virology; D010802:Phylogeny; D015995:Prevalence; D029301:Sandfly fever Naples virus / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013030:Spain / Q000453:epidemiology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014764:Viral Proteins