Development of antibodies to human embryonic stem cell antigens.
BACKGROUND
Using antibodies to specific protein antigens is the method of choice to assign and identify cell lineage through simultaneous analysis of surface molecules and intracellular markers. Embryonic stem cell research can be benefited from using antibodies specific to transcriptional factors/markers that contribute to the "stemness" phenotype or critical for cell lineage.


RESULTS
In this report, we have developed and validated antibodies (either monoclonal or polyclonal) specific to human embryonic stem cell antigens and early differentiation transcriptional factors/markers that are critical for cell differentiation into definite lineage.


CONCLUSIONS
These antibodies enable stem cell biologists to conveniently identify stem cell characteristics and to quantitatively assess differentiation.
Cai
Jingli
J
Laboratory of Neurosciences, National Institute of Aging, Baltimore, MD 21224, USA. caiji@grc.nia.nih.gov
Olson
Judith M
JM
Rao
Mahendra S
MS
Stanley
Marisa
M
Taylor
Eva
E
Ni
Hsiao-Tzu
HT
D000906:Antibodies; D000917:Antibody Formation; D000943:Antigens, Differentiation / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D019070:Cell Lineage; D004622:Embryo, Mammalian / Q000166:cytology; D006801:Humans; D013234:Stem Cells / Q000166:cytology*
D000906:Antibodies; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D015415:Biomarkers