title: Role of apoptosis in the pathogenesis of Asian and South American foot-and-mouth disease viruses in swine.
abstract: In this study, we performed experiments to evaluate the extend of the process of apoptotic cell death by foot-and-mouth disease virus (FMDV). Apoptosis can also occur in some virus-infected cells, and ability of viruses to either inhibit or promote apoptosis may influence the pathologic outcome of infection. In this study, to determine if apoptosis plays a role in the outcome of FMDV infection in swine, we evaluated apoptosis in diseased tissues collected from pigs inoculated with two different stains of FMDV (O1 Campos and O Taiwan). And host cell DNA fragmentation in diseased tissue from animals which were infected with either virus was evaluated by occurrence of a laddering pattern characteristic of apoptosis. Infection of cultured keratinocytes from swine tongue failed to demonstrate apoptosis in the first few hours of infection, suggesting that cell-to-cell correlation between viral antigen and apoptotic changes, e.g. cytokine secretions by immune system cells, could be critical to initiating apoptosis. Consistent with this finding, we were able to detect the pro-inflammatory cytokine TNF-alpha in diseased tissues. A clear difference in the pathogenicity of the two different FMDV isolates to pigs was not demonstrated in our study.
authors.affiliation: National Veterinary Research and Quarantine Service, Busan, and Gyeongsang National University, Department of Veterinary Medicine, Jinju, Korea.
authors.forename: Bok-Kyung
authors.initials: BK
authors.lastname: Ku
authors.forename: Soon-Bok
authors.initials: SB
authors.lastname: Kim
authors.forename: Oun-Kyeong
authors.initials: OK
authors.lastname: Moon
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Lyoo
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jung-Hyang
authors.initials: JH
authors.lastname: Sur
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology / Q000502:physiology*; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000502:physiology*; D005536:Foot-and-Mouth Disease / Q000503:physiopathology*; D029544:Foot-and-Mouth Disease Virus / Q000472:pathogenicity*; D007150:Immunohistochemistry / Q000662:veterinary; D020287:In Situ Nick-End Labeling / Q000662:veterinary; D015603:Keratinocytes / Q000502:physiology / Q000821:virology; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000503:physiopathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha