title: The protein expression of Streptococcus pyogenes is significantly influenced by human plasma.
abstract: During the course of infection, the common human pathogen Streptococcus pyogenes encounters plasma. We show that plasma causes S. pyogenes to rapidly remodel its cellular metabolism and virulence pathways. We also identified a variant of the major virulence factor, M1 protein, lacking 13 amino acids at the NH(2)-terminus in bacteria grown with plasma. The pronounced effect of plasma on protein expression, suggests this is an important adaptive mechanism with implications for S. pyogenes pathogenicity.
authors.affiliation: Department of Clinical Sciences, Lund University, Lund, Sweden. Bjorn.Johansson@medkem.lu.se
authors.forename: Björn P
authors.initials: BP
authors.lastname: Johansson
authors.forename: Fredrik
authors.initials: F
authors.lastname: Levander
authors.forename: Ulrich
authors.initials: U
authors.lastname: von Pawel-Rammingen
authors.forename: Tord
authors.initials: T
authors.lastname: Berggård
authors.forename: Lars
authors.initials: L
authors.lastname: Björck
authors.forename: Peter
authors.initials: P
authors.lastname: James
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D001692:Biological Transport; D002853:Chromatography, Liquid; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D013058:Mass Spectrometry; D010949:Plasma / Q000382:microbiology*; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013297:Streptococcus pyogenes / Q000378:metabolism* / Q000472:pathogenicity; D015854:Up-Regulation; D014763:Viral Matrix Proteins / Q000378:metabolism; D014774:Virulence
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D011506:Proteins; D014763:Viral Matrix Proteins