title: Bioassay-guided evaluation of anti-inflammatory and antinociceptive activities of pistachio, Pistacia vera L.
abstract: The ethanolic and aqueous extracts prepared from different parts of Pistacia vera L. (Anacardiaceae) as well as its oleoresin were evaluated for their in vivo anti-inflammatory and antinociceptive activities. Among the extracts screened, only the oleoresin was shown to possess a marked anti-inflammatory activity against carrageenan-induced hind paw edema model in mice without inducing any gastric damage at both 250 and 500 mg/kg doses whereas the rest of the extracts were totally inactive. While the oleoresin was found to display significant antinociceptive activity at 500 mg/kg dose, the ethanolic and aqueous extracts belonging to fruit, leaf, branch and peduncle of Pistacia vera did not exhibit any noticeable antinociception in p-benzoquinone-induced abdominal contractions in mice. Fractionation of the oleoresin indicated the n-hexane fraction to be active, which further led to recognition of some monoterpenes, mainly alpha-pinene (77.5%) by capillary gas chromatography-mass spectrometry (GC-MS) as well as the oleoresin itself. alpha-Pinene was also assessed for its antinociceptive and anti-inflammatory activities in the same manner and exerted a moderate anti-inflammatory effect at 500 mg/kg dose.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler 06330, Ankara, Turkey. iorhan@gazi.edu.tr
authors.forename: I
authors.initials: I
authors.lastname: Orhan
authors.forename: E
authors.initials: E
authors.lastname: Küpeli
authors.forename: M
authors.initials: M
authors.lastname: Aslan
authors.forename: M
authors.initials: M
authors.lastname: Kartal
authors.forename: E
authors.initials: E
authors.lastname: Yesilada
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D027927:Pistacia / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts