Cell death pattern in cerebellum neurons infected with Toxoplasma gondii.
There is a demand for studying the role of Toxoplasma gondii in cell death seeking aiding prevention of the disease. The neuro-pathological changes in the cerebellum cortex in case of acquired toxoplasmosis had been studied. Adult Balb C mice were infected by intra peritoneal injection of T. gondii RH strain. Immuno-histochemical expression of pro apoptotic marker Bax had been applied in parallel with Hematoxylin and Eosin stain to study the layers of cerebellum cortex. The focal necrosis in the cerebellum was expressed. Necrosis was explained on the basis of hypoxic ischemia resulting from existing vasculitis followed the infection. Purkinje cell layer was markedly affected in the form of disfiguring and focal loss of cells with apoptotic and necrotic changes. Thinning of both the molecular and internal granular layers was recorded morphometricly. Morphometric study reveals non significant change in the ratio between the viable to non viable cells in all cerebellum layers among experimental and control groups though the Purkinje cell layer was mostly affected. Statistical significant changes in depth proportion of molecular layer: Internal granular (ML: IGL) layers was noted in experimental and control group (p=0.05). Bax expression was not coexisting with the result of H & E stained cells. The hypothesis emphasizes that toxoplasmosis resist apoptosis seeking its benefit, and apoptosis followed toxoplasmosis may be due to another protein rather than Bax.
el-Sagaff
Samar
S
Department of Anatomy, Faculty of Medicine, King Abdel Aziz University, Jeddah, Saudi Arabia.
Salem
Hala Said
HS
Nichols
Wafa
W
Tonkel
Abdel Kader
AK
Abo-Zenadah
Najlaa Y A
NY
D000818:Animals; D017209:Apoptosis; D001426:Bacterial Proteins; D002531:Cerebellum / Q000166:cytology* / Q000469:parasitology* / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011689:Purkinje Cells; D011897:Random Allocation; D014122:Toxoplasma / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000276:immunology / Q000473:pathology
D001426:Bacterial Proteins