title: Sulfated glycosaminoglycans of the periurethral tissue in women with and without stress urinary incontinence, according to genital prolapse stage.
abstract: OBJECTIVE
  The objective was to determine sulfated glycosaminoglycans (GAG) of the extracellular matrix (ECM) in women with and without stress urinary incontinence according to genital prolapse stage.
  
  
  METHODS
  Periurethral tissue was obtained from 30 women who underwent surgery for urinary incontinence, for pelvic organ prolapse, or for other benign gynecologic conditions. Biopsy specimens were assessed by biochemical methods to characterize and quantify sulfated glycosaminoglycans. Measurements were made of total glycosaminoglycans, chondroitin sulfate, dermatan sulfate, and of heparan sulfate. Data were compared using the t-test.
  
  
  RESULTS
  In two groups, dermatan sulfate was the most predominant glycosaminoglycan. Women with stress urinary incontinence had significantly more total sulfated glycosaminoglycans (p<0.05) and dermatan sulfate (p<0.05) than women without stress urinary incontinence. We did not observe any differences in chondroitin sulfate and heparan sulfate.
  
  
  CONCLUSIONS
  Women with stress urinary incontinence showed quantitative and qualitative differences in the biochemical characteristics of the extracellular matrix in periurethral tissue by analysis of sulfated glycosaminoglycans, according to genital prolapse stage.
authors.affiliation: Section of Urogynecology and Pelvic Surgery, Department of Gynecology, Federal University of São Paulo, Rua dos Otonis 567, Vila Clementino, São Paulo, SP, Brazil. pfeldner@alfa.epm.br
authors.forename: Paulo Cezar
authors.initials: PC
authors.lastname: Feldner
authors.forename: Lea Mina
authors.initials: LM
authors.lastname: Kati
authors.forename: Marair Gracio Ferreira
authors.initials: MG
authors.lastname: Sartori
authors.forename: Edmund Chada
authors.initials: EC
authors.lastname: Baracat
authors.forename: Geraldo
authors.initials: G
authors.lastname: Rodrigues de Lima
authors.forename: Helena B
authors.initials: HB
authors.lastname: Nader
authors.forename: Carl P
authors.initials: CP
authors.lastname: Dietrich
authors.forename: Manoel João Batista Castello
authors.initials: MJ
authors.lastname: Girão
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D003871:Dermatan Sulfate / Q000378:metabolism*; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D012720:Severity of Illness Index; D014521:Urethra / Q000378:metabolism*; D014550:Urinary Incontinence, Stress / Q000378:metabolism* / Q000473:pathology; D014596:Uterine Prolapse / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers; D006025:Glycosaminoglycans; D003871:Dermatan Sulfate