[Remodel retinal neovascularization by overexpression of HESR-1 in vitro].
OBJECTIVE
To remodel retinal neovascularization by overexpression of HESR-1 in vitro.


METHODS
Human retinal capillary endothelial cell (HRCEC) was isolated, then HRCECs were transfected with pcDNA3.1+HESR-1. The changes of expression of KDR and occluding in HRCECs were analyzed using ELISA assay, the regulation of occluding in HRCECs by HESR-1 was evaluated by western blot.


RESULTS
Over-expression of HESR-1 down-regulated KDR expression, but occludin expression was up-regulated in HRECEs, the result also showed that the vessels permeability was reduced by HESR-1.


CONCLUSIONS
Overexpression of HESR-1 in HRCECs can inhibit the neovascularization and decrease the permeability of new vessels. It may be a promising gene therapy for intraocular neovascularization.
Tang
Shi-bo
SB
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. sbtang@gzsums.edu.cn
Li
Bin
B
Zhang
Ge
G
Chen
Jian-hong
JH
Li
Bao-jin
BJ
D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D004730:Endothelium, Vascular / Q000378:metabolism; D015870:Gene Expression; D015316:Genetic Therapy; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D009389:Neovascularization, Pathologic; D062793:Occludin; D015861:Retinal Neovascularization / Q000235:genetics*; D012171:Retinal Vessels / Q000378:metabolism; D014162:Transfection; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
D051792:Basic Helix-Loop-Helix Proteins; D018797:Cell Cycle Proteins; D008565:Membrane Proteins; D062793:Occludin; D040301:Vascular Endothelial Growth Factor Receptor-2; C485631:HEY1 protein, human; C568985:OCLN protein, human