title: The relationship of in vivo central CB1 receptor occupancy to changes in cortical monoamine release and feeding elicited by CB1 receptor antagonists in rats.
abstract: BACKGROUND
  Cannabinoid type 1 (CB(1)) receptor antagonists are reportedly effective in reducing food intake both preclinically and clinically. This may be due in part to their effects on monoamine release in the brain. The level of central CB(1) receptor occupancy underlying these neurobiological effects is unclear.
  
  
  OBJECTIVE
  We explored the relationship between in vivo CB(1) receptor occupancy in the frontal cortex and changes in both monoamine release in the medial prefrontal cortex (mPFC) and feeding behavior in rats in response to two orally administered CB(1) receptor antagonists presently in clinical trials, SR141716A (rimonabant) and SLV319.
  
  
  METHODS
  CB(1) receptor occupancy was measured using [(3)H] SR141716A, and these occupancies were related to potencies to mediate increases in dopamine (DA) and norepinephrine (NE) release measured with microdialysis and decreases in consumption of a highly palatable diet (HP).
  
  
  RESULTS
  High receptor occupancy levels (>65%) were required to detect increases in monoamine release that were achieved with 3 and 10 mg/kg of SR141716A and 10 mg/kg of SLV319 for DA and 10 mg/kg of SR141716A for NE. Decreases in HP consumption were seen at occupancies higher than 65% for SR141716A that were achieved with 3 and 10 mg/kg. In contrast, decreases in HP consumption were seen at relatively low CB(1) receptor occupancies (11%) for SLV319.
  
  
  CONCLUSIONS
  The occupancy method described here is an effective tool for interrelating central CB(1) receptor occupancy with neurobiological actions of CB(1) receptor antagonists and for furthering our understanding of the role of CB(1) receptors in central nervous system physiology and pathology.
authors.affiliation: Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285-0510, USA.
authors.forename: Anne B
authors.initials: AB
authors.lastname: Need
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Davis
authors.forename: Jesline T
authors.initials: JT
authors.lastname: Alexander-Chacko
authors.forename: Brian
authors.initials: B
authors.lastname: Eastwood
authors.forename: Eyassu
authors.initials: E
authors.lastname: Chernet
authors.forename: Lee A
authors.initials: LA
authors.lastname: Phebus
authors.forename: Dana K
authors.initials: DK
authors.lastname: Sindelar
authors.forename: George G
authors.initials: GG
authors.lastname: Nomikos
mesh_terms: D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D005247:Feeding Behavior / Q000187:drug effects*; D005625:Frontal Lobe / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D009638:Norepinephrine / Q000378:metabolism*; D010880:Piperidines / Q000494:pharmacology; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D043884:Receptor, Cannabinoid, CB1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000077285:Rimonabant; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: C482795:3-(4-chlorophenyl)-N-methyl-N'-((4-chlorophenyl)sulfonyl)-4-phenyl-4,5-dihydro-1H-pyrazole-1-carboxamidine; D010880:Piperidines; D011720:Pyrazoles; D043884:Receptor, Cannabinoid, CB1; D013449:Sulfonamides; D000077285:Rimonabant; D004298:Dopamine; D009638:Norepinephrine