Isolation of equine herpesvirus-2 from the lung of an aborted fetus.
This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause-effect relationship was established between the EHV-2 isolate and the abortion.
Galosi
Cecilia Mónica
CM
Department of Virology, Faculty of Veterinary Sciences, National University of La Plata, Buenos Aires Province, Argentina.
de la Paz
Viviana Cid
VC
Fernández
Leandro Carlos
LC
Martinez
Javier Pablo
JP
Craig
María Isabel
MI
Barrandeguy
María
M
Etcheverrrigaray
María Elisa
ME
D037881:Aborted Fetus / Q000821:virology*; D000818:Animals; D016172:DNA Fingerprinting / Q000662:veterinary; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D008168:Lung / Q000196:embryology / Q000821:virology*; D009500:Neutralization Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D018144:Rhadinovirus / Q000235:genetics / Q000302:isolation & purification*; D014412:Tumor Virus Infections / Q000662:veterinary* / Q000821:virology
D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI