Detection and characterisation of a second potyvirus from Thunberg fritillary in China.
Plants of Thunberg fritillary (Fritillaria thunbergii Miq.) from Zhejiang Province, were found to be co-infected with two distinct potyviruses. One was an isolate of the recently reported Thunberg fritillary mosaic virus (TFMV; Wei et al., (2005) Arch Virol 150: 1271-1280), while the other was a distinct virus that did not react with TFMV antiserum nor with antisera to 17 other potyviruses, except for a weak reaction with antibodies produced to soybean mosaic virus (SMV) Pinellia strain. Both viruses could be transmitted mechanically to their original host but not to any of a range of commonly used indicator plants. No local lesion host was identified that would enable the viruses to be propagated independently. The complete sequences of both viruses were determined; that of the new virus (9656 nt) had the typical genome organisation and recognised sequence motifs of a potyvirus, encoding a putative polyprotein of 351 kDa. Phylogenetic analysis, sequence comparisons, and the pattern of polyprotein cleavage sites all indicated that it was a member of the Bean common mosaic virus subgroup. The most closely related species are Soybean mosaic virus and Wisteria vein mosaic virus, with 68-69% amino acid identity between their polyproteins. This is sufficiently different for the new virus to be regarded as a distinct species, which we have tentatively named Fritillary virus Y.
Chen
J
J
Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, People's Republic of China. jchen1@mail.hz.zj.cn
Zheng
H-Y
HY
Shi
Y-H
YH
Adams
M J
MJ
Wei
C-B
CB
Lin
L
L
Chen
J-P
JP
D001483:Base Sequence; D002681:China; D004279:DNA, Viral / Q000235:genetics; D031410:Fritillaria / Q000821:virology*; D016679:Genome, Viral; D008854:Microscopy, Electron; D010802:Phylogeny; D017800:Potyvirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification
D004279:DNA, Viral; D012367:RNA, Viral