Multi-site incorporation of bioactive matrices into MEMS-based neural probes.
Methods are presented to incorporate polymer-based bioactive matrices into micro-fabricated implantable microelectrode arrays. Using simple techniques, hydrogels infused with bioactive molecules are deposited within wells in the substrate of the device. This method allows local drug delivery without increasing the footprint of the device. In addition, each well can be loaded individually, allowing spatial and temporal control over diffusion gradients in the microenvironment of the implanted neural interface probe. In vivo testing verified the following: diffusion of the bioactive molecules, integration of the bioactive molecules with the intended neural target and concurrent extracellular recording using nearby electrodes. These results support the feasibility of using polymer gels to deliver bioactive molecules to the region close to microelectrode shanks. This technique for microdrug delivery may serve as a means to intervene with the initial phases of the neuroinflammatory tissue response to permanently implanted microelectrode arrays.
Williams
Justin C
JC
Harrington Department of Bioengineering, Arizona State University, Tempe, AZ 85287, USA. j.williams@neurosurg.wisc.edu
Holecko
Matthew M
MM
Massia
Stephen P
SP
Rousche
Patrick
P
Kipke
Daryl R
DR
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000502:physiology; D016503:Drug Delivery Systems / Q000295:instrumentation* / Q000379:methods; D004567:Electrodes, Implanted; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007267:Injections / Q000295:instrumentation*; D008839:Microelectrodes; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D051381:Rats