Effects of some drugs on the activity of glucose 6-phosphate dehydrogenase from rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro.
Inhibitory effects of some drugs on glucose 6-phosphate dehydrogenase from the erythrocytes of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) were investigated. The enzyme was purified 2488-fold in a yield of 76.8% using ammonium sulfate precipitation and 2',5'-ADP Sepharose 4B affinity gel at 4 degrees C. The drugs pental sodium, MgSO4, vancomycin, metamizol, marcaine, and prilocaine all exhibited inhibitory effects on the enzyme. While MgSO4 (K(i) = 12.119 mM), vancomycin (K(i) = 1.466 mM) and metamizol (K(i) = 0.392 mM) showed competitive inhibition, pental sodium (K(i) = 0.748 mM) and marcaine (K(i) = 0.0446 mM) displayed noncompetitive inhibition.
Ciftci
Mehmet
M
Department of Chemistry, Arts and Science Faculty, Ataturk University, Erzurum, Turkey. ciftcim@atauni.edu.tr
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000097:blood*
D004791:Enzyme Inhibitors; D005954:Glucosephosphate Dehydrogenase