[Determination of uric acid by chemiluminescence].
A novel chemiluminescence method for the determination of uric acid based on its inhibitory effect on the catalysis of Co2+ for the oxidation of luminol by H2O2 is described. The proposed method is simple, very sensitive and inexpensive. The decrease of cheniluminescence intensity is linearly correlated with uric acid concentration. The calibration graph is linear over the range of 1.0 x 10(-10) - 7.0 x 10(-6) mol x L(-1) and the detection limit is 1.1 x 10(-11) mol x L(-1). The relative standard deviation (RSD) for 5.0 x 10(-8) is 1.9% (n=4). The method has been successfully applied to the determination of uric acid in human urine and serum samples.
Liu
Er-bao
EB
School of Chemistry and Life Sciences, Tianjin Normal University, Tianjin 300074, China.
Wei
Hong-qing
HQ
D002384:Catalysis; D003035:Cobalt / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D008165:Luminol / Q000737:chemistry; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014527:Uric Acid / Q000032:analysis / Q000097:blood* / Q000652:urine*
D014527:Uric Acid; D003035:Cobalt; D008165:Luminol; D006861:Hydrogen Peroxide