Biocompatibility and degradation of aliphatic segmented poly(ester amide)s: in vitro and in vivo evaluation.
Aliphatic segmented poly(ester amide)s, comprising a crystallizable amide phase and a flexible amorphous ester phase, were investigated for potential use in biomedical applications. By varying the amide content and the type of crystallizable amide segments, the polymer's thermal and mechanical properties can readily be tuned. Polymers with 25 and 50 mol % of amide content are noncytotoxic and sustain growth of fibroblasts onto polymer films. The in vitro degradation of these polymers was followed in PBS (pH 7.4) at 37 degrees C up to 7 months. The poly(ester amide)s showed the characteristics of bulk degradation with a gradual decrease in molecular weight and almost no mass loss. The in vivo degradation of these polymers, followed by subcutaneous implantation in rats up to 6 weeks, was slow and similar to the in vitro degradation. The tissue response upon implantation was followed over 6 weeks. A mild foreign-body reaction, characterized by the presence of macrophages, and sporadically a lymphocyte, were observed in the first week of implantation. After 6 weeks the implant site is characterized by fibrous encapsulation with no signs of inflammation. The poly(ester amide)s tested are biocompatible, but their in vitro as well as in vivo degradation is very slow.
Lips
Priscilla A M
PA
Institute for Biomedical Technology, BMTIm and Department of Polymer Chemistry and Biomaterials, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
van Luyn
Marja J A
MJ
Chiellini
Federica
F
Brouwer
Linda A
LA
Velthoen
Ingrid W
IW
Dijkstra
Piet J
PJ
Feijen
Jan
J
D000577:Amides / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials; D002152:Calorimetry, Differential Scanning; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D003460:Crystallization; D005549:Foreign-Body Reaction; D006868:Hydrolysis; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties; D013718:Tensile Strength; D014783:Viscosity
D000577:Amides; D001672:Biocompatible Materials; D011091:Polyesters