Phytochelatin plays a role in UV-B tolerance in N2-fixing cyanobacterium Anabaena doliolum.
To study the role of Cd-induced phytochelatins in UV-B tolerance, lipid peroxidation, antioxidative enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase), glutathione arid phytochelatin contents were measured in buthionine sulphoximine treated and untreated cells of Anabaena doliolum. Cd-pretreatment of the cyanobacterium reduced the lipid peroxidation as well as the antioxidative enzymes in comparison to UV-B treatment alone, whereas the phytochelatin content demonstrated an increase. In contrast to this, buthionine sulphoximine-induced inhibition of phytochelatin synthase, dramatically decreased the Cd-induced co-tolerance against UV-B, hence demonstrating that phytochelatin not only protects the cyanobacterium from heavy metal but participates in UV-B tolerance as well.
Bhargava
Poonam
P
Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras Hindu University, India.
Srivastava
Ashish Kumar
AK
Urmil
Snigdha
S
Rai
Lal Chand
LC
D000222:Adaptation, Physiological / Q000502:physiology*; D017033:Anabaena / Q000254:growth & development / Q000502:physiology / Q000528:radiation effects*; D005978:Glutathione / Q000502:physiology*; D009586:Nitrogen Fixation; D054811:Phytochelatins; D014466:Ultraviolet Rays
D054811:Phytochelatins; D005978:Glutathione