[UCPs and PPARgamma2 mRNA in diet induced obesity resistant rats].
OBJECTIVE
To explore the expression of uncoupling proteins (UCP) and peroxisome proliferator activated receptorgamma2 (PPARgamma2) mRNA in white adipose of high-fat diet induced obesity resistant rats.


METHODS
Thirty-six female SD rats were randomly divided into high-fat diet group (n = 27) and chow fed control group (n = 9), and given either high-fat diet or chow for thirteen weeks. Then the high-fat diet group was subdivided into Dietary Induced Obesity (DIO) and Dietary Induced Obesity Resistant (DIO-R) group according to the final body weight. Basic parameters, fasting blood glucose (FBG), blood lipid levels were measured and compared. Reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the expression of UCP2, UCP3, and PPARgamma2 mRNA in white adipose.


RESULTS
Adipose deposits, body fat percent, TC, and TG conceration of both DIO-R and DIO groups were significantly higher than those in control group, while body weight, body fat percent and TG level were significantly lower in DIO-R than DIO rats. The expression of UCP2 and UCP3 mRNA in white adipose of DIO-R rats were significantly higher than those of DIO and control group. The expression of PPARgamma2 mRNA in white adipose of DIO-R group was significantly lower than that in DIO group.


CONCLUSIONS
High-fat diet induced obesity resistance were closely associated with the increased UCP2, UCP3, and decreased PPARgamma2 mRNA in white adipose.
Yang
Nian-Hong
NH
Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Wang
Chong-Jian
CJ
Xu
Ming-Jia
MJ
Hu
Xue-Feng
XF
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D009765:Obesity / Q000209:etiology / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3
D004041:Dietary Fats; D007473:Ion Channels; D024101:Mitochondrial Proteins; D047495:PPAR gamma; D012333:RNA, Messenger; C000604391:Ucp2 protein, rat; C000604394:Ucp3 protein, rat; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3