T-DNA tagging and characterization of a cryptic root-specific promoter in Arabidopsis.
From a T-DNA tagged Arabidopsis population, a line, M-57 showing GUS (beta-glucuronidase) expression in the vascular regions of young roots was identified. Southern analysis revealed presence of a single T-DNA insert. Using inverse PCR, the plant sequence flanking the T-DNA insertion was cloned. The insertion was identified to be in the intergenic area between loci At4G13940 and At4G13930, coding for SAHH (S-Adenosyl-l-Homocysteine Hydrolase) and SHMT (Serine Hydroxy Methyl Transferase) genes, respectively. A 452-bp fragment immediately upstream of the T-DNA insertion when cloned and mobilized as a GUS fusion was capable of driving a similar root-specific expression of reporter gene in transgenic Arabidopsis plants and their progenies. This cryptic promoter element does not show the presence of any known root-specific promoter element.
Sivanandan
C
C
National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110012, India.
Sujatha
T P
TP
Prasad
Anand Mohan
AM
Resminath
R
R
Thakare
Dhiraj R
DR
Bhat
S R
SR
Srinivasan
D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D021901:DNA, Intergenic; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D050436:Regulatory Elements, Transcriptional; D016415:Sequence Alignment; D024363:Transcription Initiation Site
D004269:DNA, Bacterial; D021901:DNA, Intergenic; D018744:DNA, Plant; C036483:T-DNA; D005966:Glucuronidase