Biocatalytic amide reduction using Clostridium sporogenes.
Washed cells of Clostridium sporogenes reduced benzamide (up to 20 mM: ) to benzylamine in yields up to 73% using H2 as electron donor with less than 10 g biocatalyst/l over 24 h. Product formation exhibited complex kinetics, with a lag before benzylamine production began. Very little substrate was hydrolysed since the maximum yield of benzoic acid was only 9% of the substrate added. Boiled cells were inactivated thus confirming that amide reduction was enzyme-catalysed.
Dipeolu
Olutosin
O
School of Chemical Engineering and Analytical Science, University of Manchester, P.O. Box 88, M60 1QD, Manchester, UK.
Gardiner
John
J
Stephens
Gill
G
D000577:Amides / Q000378:metabolism; D001549:Benzamides / Q000378:metabolism*; D001596:Benzylamines / Q000302:isolation & purification* / Q000378:metabolism*; D002384:Catalysis; D018929:Cell Culture Techniques / Q000379:methods*; D003013:Clostridium / Q000378:metabolism*; D010084:Oxidation-Reduction
D000577:Amides; D001549:Benzamides; D001596:Benzylamines; C037689:benzamide; C030796:benzylamine