title: Enhancing the synthetic utility of aldolase antibody 38C2.
abstract: Three-phase partitioning (TPP) treated aldolase antibody 38C2 was evaluated for aldol reaction between p-nitrobenzaldehyde and acetone to give 4-(4'-nitrophenyl)-4-hydroxy-2-butanone. While TPP-treated 38C2 transformed 65% of p-nitrobenzaldehyde, the untreated 38C2 gave only 24% transformation in 18 h at 25 degrees C. However, since TPP-treated 38C2 also gave an additional (unidentified) product, its synthetic utility was limited. Crosslinked aggregate of 38C2, however, gave the biocatalyst which gave a single product and could be reused at 40 degrees C five times without loss of activity.
authors.affiliation: Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
authors.forename: Kalyani
authors.initials: K
authors.lastname: Mondal
authors.forename: Namakkal G
authors.initials: NG
authors.lastname: Ramesh
authors.forename: Ipsita
authors.initials: I
authors.lastname: Roy
authors.forename: Munishwar N
authors.initials: MN
authors.lastname: Gupta
mesh_terms: D000096:Acetone / Q000737:chemistry; D001547:Benzaldehydes / Q000737:chemistry; D002074:Butanones / Q000138:chemical synthesis* / Q000737:chemistry; D002384:Catalysis; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D015394:Molecular Structure; D013997:Time Factors
chemical_list: C509737:4-(4'-nitrophenyl)-4-hydroxy-2-butanone; D001547:Benzaldehydes; D002074:Butanones; D007140:Immunoglobulin Fab Fragments; D000096:Acetone; C029915:4-nitrobenzaldehyde; C109536:antibody aldolase; D005634:Fructose-Bisphosphate Aldolase