title: Experimental tests to definitively determine ubiquitylation of a substrate.
abstract: Ubiquitin-mediated proteolysis is a major pathway of protein degradation that regulates numerous cellular processes. An understanding of the circumstances that contribute to the ubiquitylation of a specific protein can yield vast insight into its regulation. This article examines multiple procedures that explain whether a protein is ubiquitylated and suggests methods to investigate the factors that specifically target the substrate for ubiquitylation, as well as the site of ubiquitin conjugation.
authors.affiliation: Department of Pathology, New York University School of Medicine and NYU Cancer Institute, New York, USA.
authors.forename: Joanna
authors.initials: J
authors.lastname: Bloom
authors.forename: Michele
authors.initials: M
authors.lastname: Pagano
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D006868:Hydrolysis; D047468:Immunoprecipitation; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D025801:Ubiquitin