title: [Interleukin 10 gene -627 polymorphisms in Chinese patients with early-onset coronary heart disease].
abstract: OBJECTIVE
  To investigate the relationship between interleukin 10 (IL10) gene -627 polymorphisms and serum IL10 level and early-onset coronary heart disease (CHD).
  
  
  METHODS
  The genotype and allele frequency of IL10 gene -627 site was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). DNA samples were obtained from 163 patients with CHD and 112 controls. Serum IL10 level was detected by ELISA.
  
  
  RESULTS
  No significant difference was found in the distribution of IL10 genotype and allele frequency between the healthy controls and the patients with CHD; Chi-square values were 1.9324 and 1.5703 respectively, P > 0.05. Stratification analyses based on different sex still found no significant difference in the distribution of IL10 genotype and allele frequency between the healthy controls and the CHD patients; the Chi-square values in male groups were 1.2708 versus 0.8595, and in female groups were 0.8254 versus 0.7127, P > 0.05. Serum IL10 level showed significant differences among AA genotype, AC genotype and CC genotype, but no significant difference was noted between healthy controls and CHD patients.
  
  
  CONCLUSIONS
  These results suggest that IL10 gene -627 polymorphisms are not associated with an increased risk of CHD, but it might assume a role in IL10 gene expression.
authors.affiliation: Laboratory of Marine Genetics and Breeding, Division of Life Science and Biotechnology, Ocean University of China, Qingdao, Shandong, P. R. China. sdqdwyy@126.com
authors.forename: Yun-ying
authors.initials: YY
authors.lastname: Wang
authors.forename: Xin-juan
authors.initials: XJ
authors.lastname: Yu
authors.forename: Pei-lin
authors.initials: PL
authors.lastname: Wang
authors.forename: Zhen-min
authors.initials: ZM
authors.lastname: Bao
mesh_terms: D000328:Adult; D017668:Age of Onset; D044466:Asian People / Q000235:genetics; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D003327:Coronary Disease / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D016753:Interleukin-10