The influence of sediment resuspension on the degradation of phenanthrene in flow-through microcosms.
The effect of sediment resuspension on the mineralization of phenanthrene was examined in microcosms and sediment slurries. In computer-controlled, flow-through microcosms, 14C-phenanthrene-amended sediments were resuspended into overlying oxic water at frequencies of 12, 4, 1, 0.25 and 0 d(-1). In slurry bottle experiments 14C-phenanthrene-amended sediments were continuously resuspended under oxic (excess air headspace) and anoxic (N2 headspace) conditions and mineralization was measured at periods from 2 h to 7 days. Our main findings were: (1) mineralization rate constants from the microcosms ranged from 0.001 to 0.01 d(-1) and increased with frequency of resuspension, (2) these rates fell between those measured in oxic and anoxic slurries and were predicted within a factor of 2.5 by a model in which mineralization depended on the degree of oxygen exposure, and (3) the phenanthrene-degrading bacterial community was more active in resuspended sediments incubated in the microcosms than in sediments which were not resuspended, or which were stored under refrigeration. We conclude from these experiments that the effects of sediment resuspension on phenanthrene degradation are consistent with a primary role of average oxygen exposure, and also an alteration in the PAH-degrading activity of microbial populations.
LeBlanc
Lawrence A
LA
Marine Sciences Research Center, State University of New York at Stony Brook, Stony Brook, NY 11794-5000, USA. Lawrence.leblanc@umit.maine.edu
Gulnick
Jeanne D
JD
Brownawell
Bruce J
BJ
Taylor
Gordon T
GT
D018407:Bacterial Physiological Phenomena; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000032:analysis; D002250:Carbon Radioisotopes / Q000032:analysis; D018504:Ecological Systems, Closed; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D019015:Geologic Sediments / Q000737:chemistry*; D008903:Minerals / Q000378:metabolism; D010100:Oxygen / Q000032:analysis / Q000737:chemistry; D010616:Phenanthrenes / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
D002250:Carbon Radioisotopes; D008903:Minerals; D010616:Phenanthrenes; D014874:Water Pollutants, Chemical; D002245:Carbon Dioxide; C031181:phenanthrene; D010100:Oxygen