KESTREL: a powerful method for identifying the physiological substrates of protein kinases.
The identification of all the substrates of every protein kinase is one of the major challenges of post-genomic research. Here we review a powerful method for tackling this problem that we have developed over the last 5 years. The method has so far been used to identify novel substrates for eight different protein kinases, demonstrating that it is of general utility. Importantly, the method can be used to identify distinct physiological substrates of protein kinases, such as PKB (protein kinase B) and SGK (serum- and glucocorticoid-induced kinase), that are closely related in structure and have similar specificity determinants.
Cohen
Philip
P
MRC Protein Phosphorylation Unit, MSI/WTB Complex, Dow Street, Dundee DD1 5EH, UK. p.cohen@dundee.ac.uk
Knebel
Axel
A
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D010449:Peptide Mapping; D010766:Phosphorylation; D011494:Protein Kinases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D013379:Substrate Specificity
D011494:Protein Kinases