title: Effect of SsrA (tmRNA) tagging system on translational regulation in Streptomyces.
abstract: ssrA genes encoding tmRNA with transfer and messenger RNA functions are ubiquitous in bacteria. In a process called trans-translation, tmRNA enters a stalled ribosome and allows release of the original mRNA, then tmRNA becomes the template for translation of a short tag that signals for proteolytic degradation. We provide here the first evidences that the tmRNA tagging system (ssrA and cohort smpB) is active in Streptomyces. Transcription of the genes was shown and construction of a genetic probe allowed detection of a tmRNA-tagged peptide. Obtention of ssrA and smpB mutants of Streptomyces lividans showed that the ssrA system is dispensable in Streptomyces. Morphologies of the mutants colonies were similar to the wild type, thus tmRNA-mediated tagging does not seem to have, under conditions used, a significant effect in the Streptomyces differentiation.
authors.affiliation: UP Génétique Bactérienne et Différenciation, Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Braud
authors.forename: Celine
authors.initials: C
authors.lastname: Lavire
authors.forename: Audrey
authors.initials: A
authors.lastname: Bellier
authors.forename: Philippe
authors.initials: P
authors.lastname: Mazodier
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology; D010450:Endopeptidases / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D014176:Protein Biosynthesis / Q000502:physiology*; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000502:physiology*; D048372:Streptomyces coelicolor / Q000502:physiology; D048170:Streptomyces lividans / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; C096222:tmRNA; D012343:RNA, Transfer; D010450:Endopeptidases; C072135:C-terminal processing peptidase