Antisense-mediated inhibition of acid trehalase (ATH1) gene expression promotes ethanol fermentation and tolerance in Saccharomyces cerevisiae.
Acid trehalase gene (ATH1) expression was decreased using the antisense-RNA technique in Saccharomyces cerevisiae. The 500 bp DNA fragments containing anti-ATH1 gene between +1 and +500 were amplified using PCR and fused to yeast ADH1, CYC1 and ATH1 promoters. Yeast cells harboring the recombinant plasmids had a low activity of acid trehalase and promoted ethanol fermentation compared to the control yeast cells harboring the vector plasmid only. The recombinant yeast had a high viability with 8% (v/v) ethanol.
Jung
Young-Ji
YJ
Department of Life and Food Sciences, Kyungpook National University, 1370 Sangkyuk, 702-701 Daegu, Korea.
Park
Heui-Dong
HD
D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D000431:Ethanol / Q000378:metabolism* / Q000494:pharmacology; D005285:Fermentation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D016372:RNA, Antisense / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic; D014198:Trehalase
D016372:RNA, Antisense; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; C097090:ATH1 protein, S cerevisiae; D014198:Trehalase