title: IS6110 restriction fragment length polymorphism of Mycobacterium tuberculosis isolates from an area of Casablanca, Morocco.
abstract: Using IS6110 RFLP, 61 isolates recovered from new cases of pulmonary tuberculosis (TB) were compared from September to December 1999 in Casablanca, Morocco, a city with a high incidence of TB. The majority of the isolates (92%) harboured 6-14 copies of IS6110. The minimal fraction of patients in groups of recently acquired infection is 13.1%. This preliminary study showed that IS6110 RFLP is a suitable method for finger-printing Mycobacterium tuberculosis in Casablanca. The unexpectedly low level of recent transmission of TB found in this study deserves further studies involving higher numbers of isolates recovered during a longer recruitment period.
authors.affiliation: Microbiology Laboratory, Ibn Rochd University Hospital, Casablanca, Morocco.
authors.forename: O
authors.initials: O
authors.lastname: Diraa
authors.forename: N
authors.initials: N
authors.lastname: Elmdaghri
authors.forename: L
authors.initials: L
authors.lastname: Laaboudi
authors.forename: M
authors.initials: M
authors.lastname: Boudouma
authors.forename: M C
authors.initials: MC
authors.lastname: Gutiérrez
authors.forename: M
authors.initials: M
authors.lastname: Benbachir
mesh_terms: D006801:Humans; D009018:Morocco; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D014397:Tuberculosis, Pulmonary / Q000382:microbiology