title: FpvA-mediated ferric pyoverdine uptake in Pseudomonas aeruginosa: identification of aromatic residues in FpvA implicated in ferric pyoverdine binding and transport.
abstract: A number of aromatic residues were seen to cluster in the upper portion of the three-dimensional structure of the FpvA ferric pyoverdine receptor of Pseudomonas aeruginosa, reminiscent of the aromatic binding pocket for ferrichrome in the FhuA receptor of Escherichia coli. Alanine substitutions in three of these, W362, W391, and F795, markedly compromised ferric pyoverdine binding and transport, consistent with a role of FpvA in ferric pyoverdine recognition.
authors.affiliation: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
authors.forename: Jiang-Sheng
authors.initials: JS
authors.lastname: Shen
authors.forename: Valérie
authors.initials: V
authors.lastname: Geoffroy
authors.forename: Shadi
authors.initials: S
authors.lastname: Neshat
authors.forename: Zongchao
authors.initials: Z
authors.lastname: Jia
authors.forename: Allison
authors.initials: A
authors.lastname: Meldrum
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Meyer
authors.forename: Keith
authors.initials: K
authors.lastname: Poole
mesh_terms: D019943:Amino Acid Substitution; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D001692:Biological Transport; D004252:DNA Mutational Analysis; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000502:physiology; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009842:Oligopeptides / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011991:Receptors, Virus / Q000737:chemistry / Q000502:physiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; C082326:FpvA protein, Pseudomonas aeruginosa; D009842:Oligopeptides; D011991:Receptors, Virus; C042453:pyoverdin