Methanolic extract of Pterocarpus santalinus induces apoptosis in HeLa cells.
Ptercarpus santalinus (Fabaceae) has been used as a folk remedy in Korea, and it has been shown to exhibit antiinflammations, antiulcers and anticancer effects. In this study, therefore, we report the cytotoxic activity and the mechanism of cell death exhibited by the methanol extract of Ptercarpus santalinus (MEPS) against human cervical adenocarcinoma cell line, HeLa. Treatment of HeLa cells with various concentrations of MEPS resulted in growth inhibition and induction of apoptosis in a dose-dependent manner as determined by cell viability, chromatin condensation, DNA fragmentation and sub-G1 phase accumulation. In Western blot analysis, apoptosis in the HeLa cells was associated with the release of cytochrome C from mitochondria into the cytosol, activation of caspases-3, -8, -9 and proteolytic cleavage of PARP. These results suggest that MEPS exhibits antiproliferative effect on HeLa cells via apoptosis, and it may be a potential candidate in field of anticancer drug discovery.
Kwon
H J
HJ
Department of Life Science and Biotechnology, College of Natural Science, Dongeui University, Busan 614-714, Republic of Korea.
Hong
Y K
YK
Kim
K H
KH
Han
C H
CH
Cho
S H
SH
Choi
J S
JS
Kim
Byung-Woo
BW
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D015536:Down-Regulation; D004789:Enzyme Activation; D006367:HeLa Cells; D006801:Humans; D006868:Hydrolysis; D000432:Methanol / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D035721:Pterocarpus / Q000737:chemistry*
D010936:Plant Extracts; D011065:Poly(ADP-ribose) Polymerases; D020169:Caspases; D000432:Methanol