Preparative electrophoresis with on-column optical fiber monitoring and direct elution into a minimized volume.
A "column-format" preparative electrophoresis device which obviates the need for gel extraction or secondary electro-elution steps is described. Separated biomolecules are continuously detected and eluted directly into a minimal volume of free solution for subsequent use. An optical fiber allows the species of interest to be detected just prior to elution from the gel column, and a small collection volume is created by addition of an ion-exchange membrane near the end of the column.
Jackson
George W
GW
Department of Chemical Engineering, University of Houston, Houston, TX, 77204, USA.
Willson
Richard
R
D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000235:genetics*; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D009862:Online Systems; D055100:Optical Fibers; D012329:RNA, Bacterial / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods
D012329:RNA, Bacterial