[Effect of radix bupleuri extract on cardiomyocyte apoptosis related genes expression in mouse infected with coxsackie virus B3m].
OBJECTIVE
To explore the mechanism of Radix Bupleuri (RB) extract in regulating cardiomyocyte apoptosis. Methods The BALB/c mouse inoculated with the cardiomyocytophilic strain Coxsackie virus B3m (CVB3m) by peritoneal injection was adopted as viral myocarditis model. The experimental mice were divided into the normal control (A), the model (B) and the RB (C) groups. Mice in Group C were given 0.2 ml / 10 g of RB extract, 2 mg/ml in concentration by gastrogavage once daily, while equal volume of normal saline was given by gastrogavage to mice in Group A and B. All the mice were sacrificed on the 10th day after modeling. The cardiomyocyte apoptosis was detected by TDT/Dutp nick end labelling (TUNEL). The expression of Fas Bcl-2, and Caspase3 protein in myocardium was determined by immunohistochemistry and reverse-transcription polymerse chain reaction (RT-PCR).


RESULTS
The apoptotic cell was not found in Group A, but Fas, Bcl-2 and Caspase3 were expressed in different degree. In Group B, a lot of apoptotic cells could be seen, with expression of Fas, Bcl-2 and Caspase3 stronger than those in Group A (P < 0.01). Compared with Group B, the apoptotic cardiomyocyte in Group C was significantly reduced (P < 0.01), with the expression of Fas and Caspase3 significantly lowered but expression of Bcl-2 increased slightly.


CONCLUSIONS
RB extract could inhibit the cardiomyocyte apoptosis in animal with viral myocarditis, obviously down-regulate the expression of Fas and Caspase3, and slightly up-regulate Bcl-2 expression.
Wang
Xue-feng
XF
Department of Clinnical Pharmacology, China Medical University, Shenyang. lnzywxf@tom.com
Zhou
Li
L
Nan
Chung-hong
CH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis / Q000235:genetics; D029972:Bupleurum; D053148:Caspase 3 / Q000096:biosynthesis / Q000235:genetics; D003384:Coxsackievirus Infections / Q000378:metabolism* / Q000473:pathology; D004770:Enterovirus; D005260:Female; D015870:Gene Expression; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics
D051017:Apoptosis Regulatory Proteins; C051274:Bupleurum root extract; C498892:Faim protein, mouse; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D053148:Caspase 3