title: Investigation of the binding geometry of a peripheral membrane protein.
abstract: A growing number of modules including FYVE domains target key signaling proteins to membranes through specific recognition of lipid headgroups and hydrophobic insertion into bilayers. Despite the critical role of membrane insertion in the function of these modules, the structural mechanism of membrane docking and penetration remains unclear. In particular, the three-dimensional orientation of the inserted proteins with respect to the membrane surface is difficult to define quantitatively. Here, we determined the geometry of the micelle penetration of the early endosome antigen 1 (EEA1) FYVE domain by obtaining NMR-derived restraints that correlate with the distances between protein backbone amides and spin-labeled probes. The 5- and 14-doxyl-phosphatidylcholine spin-labels were incorporated into dodecylphosphocholine (DPC) micelles, and the reduction of amide signal intensities of the FYVE domain due to paramagnetic relaxation enhancement was measured. The vector of the FYVE domain insertion was estimated relative to the molecular axis by minimizing the paramagnetic restraints obtained in phosphatidylinositol 3-phosphate (PI3P)-enriched micelles containing only DPC or mixed with phosphatidylserine (PS). Additional distance restraints were obtained using a novel spin-label mimetic of PI(3)P that contains a nitroxyl radical near the threitol group of the lipid. Conformational changes indicative of elongation of the membrane insertion loop (MIL) were detected upon micelle interaction, in which the hydrophobic residues of the loop tend to move deeper into the nonpolar core of micelles. The micelle insertion mechanism of the FYVE domain defined in this study is consistent with mutagenesis data and chemical shift perturbations and demonstrates the advantage of using the spin-label NMR approach for investigating the binding geometry by peripheral membrane proteins.
authors.affiliation: Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, Colorado 80045, USA.
authors.forename: Roman
authors.initials: R
authors.lastname: Brunecky
authors.forename: Stephanie
authors.initials: S
authors.lastname: Lee
authors.forename: Piotr W
authors.initials: PW
authors.lastname: Rzepecki
authors.forename: Michael
authors.initials: M
authors.lastname: Overduin
authors.forename: Glenn D
authors.initials: GD
authors.lastname: Prestwich
authors.forename: Andrei G
authors.initials: AG
authors.lastname: Kutateladze
authors.forename: Tatiana G
authors.initials: TG
authors.lastname: Kutateladze
mesh_terms: D006801:Humans; D008433:Mathematics; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008823:Micelles; D008958:Models, Molecular; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013113:Spin Labels; D033921:Vesicular Transport Proteins
chemical_list: D008565:Membrane Proteins; D008823:Micelles; D013113:Spin Labels; D033921:Vesicular Transport Proteins; C093846:early endosome antigen 1