title: Comparison of the acute response to meals enriched with cis- or trans-fatty acids on glucose and lipids in overweight individuals with differing FABP2 genotypes.
abstract: Trans-fatty acids have been implicated as a risk factor for cardiovascular disease and diabetes. In addition, a polymorphism at codon 54 (Ala54Thr) in the fatty acid-binding protein 2 (FABP2) gene has been suggested to modify an interaction between dietary fat and insulin sensitivity. We examined the postprandial metabolic profiles after meals enriched with C18:1trans- relative to a similar meal with C18:1cis-fatty acid in individuals who were either FABP2 Ala54 homozygotes or Thr54 carriers. Moderately overweight men and women ate 2 breakfast test meals, separated by 1 week, each providing 40% of their daily energy requirement and containing 50% of energy as fat. In one meal, 10% of energy was from C18:1trans, and in the other meal, the C18:1trans was replaced with C18:1cis. Metabolic parameters were assessed during an 8-hour period. Insulin and C-peptide levels increased more after the C18:1trans meal, and this was associated with a greater fall in free fatty acids. Postprandial glucose levels and oxidation of fatty acids and carbohydrate were not different between the 2 test meals. The Thr54 allele for FABP2 increased the rise in postprandial glucose but not triacylglycerols. Fractional triacylglycerol synthetic rates were higher after consumption of the C18:1trans meal relative to the C18:1cis meal only in Thr54 carriers. These data show that a single meal enriched with C18:1trans-fatty acids can significantly increase insulin resistance, and that in the presence of the FABP2 Thr54 allele, may contribute to increased partitioning of glucose to triacylglycerols and insulin resistance.
authors.affiliation: Pennington Biomedical Research Center, 6400 Perkins Rd, Baton Rouge, LA 70808, USA. lefevrm@pbrc.edu
authors.forename: Michael
authors.initials: M
authors.lastname: Lefevre
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Lovejoy
authors.forename: Steven R
authors.initials: SR
authors.lastname: Smith
authors.forename: James P
authors.initials: JP
authors.lastname: Delany
authors.forename: Catherine
authors.initials: C
authors.lastname: Champagne
authors.forename: Marlene M
authors.initials: MM
authors.lastname: Most
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Denkins
authors.forename: Lilian
authors.initials: L
authors.lastname: de Jonge
authors.forename: Jennifer
authors.initials: J
authors.lastname: Rood
authors.forename: George A
authors.initials: GA
authors.lastname: Bray
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000032:analysis*; D002096:C-Peptide / Q000032:analysis; D004041:Dietary Fats / Q000008:administration & dosage*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005838:Genotype; D006801:Humans; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D019518:Postprandial Period; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D002096:C-Peptide; D004041:Dietary Fats; C493404:FABP2 protein, human; D050556:Fatty Acid-Binding Proteins; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D008055:Lipids; D014280:Triglycerides