title: Characterization of the orf1glnKamtB operon of Herbaspirillum seropedicae.
abstract: Herbaspirillum seropedicae is an endophytic nitrogen-fixing bacterium that colonizes economically important grasses. In this organism, the amtB gene is co-transcribed with two other genes: glnK that codes for a PII-like protein and orf1 that codes for a probable periplasmatic protein of unknown function. The expression of the orf1glnKamtB operon is increased under nitrogen-limiting conditions and is dependent on NtrC. An amtB mutant failed to transport methylammonium. Post-translational control of nitrogenase was also partially impaired in this mutant, since a complete switch-off of nitrogenase after ammonium addition was not observed. This result suggests that the AmtB protein is involved in the signaling pathway for the reversible inactivation of nitrogenase in H. seropedicae.
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, 81531-990, Curitiba, Brazil.
authors.forename: Lilian
authors.initials: L
authors.lastname: Noindorf
authors.forename: Fabiane G M
authors.initials: FG
authors.lastname: Rego
authors.forename: Valter A
authors.initials: VA
authors.lastname: Baura
authors.forename: Rose A
authors.initials: RA
authors.lastname: Monteiro
authors.forename: Roseli
authors.initials: R
authors.lastname: Wassem
authors.forename: Leonardo M
authors.initials: LM
authors.lastname: Cruz
authors.forename: Liu U
authors.initials: LU
authors.lastname: Rigo
authors.forename: Emanuel M
authors.initials: EM
authors.lastname: Souza
authors.forename: Maria B R
authors.initials: MB
authors.lastname: Steffens
authors.forename: Fabio O
authors.initials: FO
authors.lastname: Pedrosa
authors.forename: Leda S
authors.initials: LS
authors.lastname: Chubatsu
mesh_terms: D001483:Base Sequence; D027682:Cation Transport Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D042803:Herbaspirillum / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames; D009876:Operon / Q000235:genetics*; D051922:PII Nitrogen Regulatory Proteins / Q000235:genetics*
chemical_list: D027682:Cation Transport Proteins; D051922:PII Nitrogen Regulatory Proteins