title: Immunolocalization of cyclin D1 in the developing lens of c-maf -/- mice.
abstract: The maf gene encodes a transcription factor protein containing a typical basic/leucine zipper domain structure, a motif for protein dimerization and DNA binding. It has been demonstrated that maf family genes have important roles in embryonic development and cellular differentiation. In this study, localization of cyclin D1, one of the cell cycle-related molecules, was examined immunohistochemically in developing lens cells of c-maf knockout (-/-) mice. At embryonic day 14 in wild-type mice, lens cells consisted of round epithelial cells in a single layer and regularly arranged elongated lens cells, indicating primary lens fiber cells. Cyclin D1-positive nuclei were observed in the lens epithelial cells, whereas cyclin D1 was not detected in the primary lens fiber cells. In c-maf -/- mice, a variety of round epithelial cells were located in the anterior and posterior lens. Many cyclin D1-positive nuclei were observed in lens epithelial cells as well as posterior lens cells. These results are consistent with c-maf playing a role in the regulation of cyclin D1 in developing lens cells.
authors.affiliation: Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, N15 W7, Kita-ku, Sapporo 060-8638, Japan. kaseron@med.hokudai.ac.jp
authors.forename: Satoru
authors.initials: S
authors.lastname: Kase
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yoshida
authors.forename: Masaharu
authors.initials: M
authors.lastname: Sakai
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ohgami
authors.forename: Kenji
authors.initials: K
authors.lastname: Shiratori
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Kitaichi
authors.forename: Yukari
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Harada
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ohno
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D019938:Cyclin D1 / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000737:chemistry* / Q000166:cytology / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D051257:Proto-Oncogene Proteins c-maf / Q000235:genetics* / Q000502:physiology*
chemical_list: D051257:Proto-Oncogene Proteins c-maf; D019938:Cyclin D1