title: Establishment of a pregnancy following intravaginal insemination with epididymal semen from a dog castrated due to benign prostatic hyperplasia.
abstract: Benign prostatic hyperplasia was diagnosed in an American Staffordshire Terrier of high breeding value presenting concurrent haematuria. Castration as a treatment was synchronized with the oestrus cycle of a bitch selected for insemination. After castration the cauda epididymis was flushed with Gent semen extender and collected spermatozoa were filtered and analysed by Hamilton Thorn computer assisted sperm analysis. A total of 7 ml semen containing 742 x 10(6) spermatozoa with 76.5% mean motility was used for insemination. Intravaginal insemination of the bitch was performed with an insemination catheter for dogs (Kruuse, Marslev, Denmark) on the day when plasma progesterone levels reached 9.9 ng/ml. Normal pregnancy without complications resulted in eight live-born puppies 63 days after insemination. This is the first report of a normal pregnancy and birth of puppies from a bitch inseminated with epididymal semen obtained from a dog affected by benign prostate hyperplasia.
authors.affiliation: Clinic for Reproduction and Equine Medicine, Veterinary Faculty, University of Ljubljana, Gerbiceva 60, 1000 Ljubljana, Slovenia. primoz.klinc@vf.uni-lj.si
authors.forename: P
authors.initials: P
authors.lastname: Klinc
authors.forename: G
authors.initials: G
authors.lastname: Majdic
authors.forename: N
authors.initials: N
authors.lastname: Sterbenc
authors.forename: N
authors.initials: N
authors.lastname: Cebulj-Kadunc
authors.forename: J
authors.initials: J
authors.lastname: Butinar
authors.forename: M
authors.initials: M
authors.lastname: Kosec
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000601:surgery*; D004285:Dogs; D005260:Female; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D008297:Male; D009919:Orchiectomy / Q000662:veterinary*; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000662:veterinary; D011470:Prostatic Hyperplasia / Q000601:surgery / Q000662:veterinary*; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*