The protein expression of Streptococcus pyogenes is significantly influenced by human plasma.
During the course of infection, the common human pathogen Streptococcus pyogenes encounters plasma. We show that plasma causes S. pyogenes to rapidly remodel its cellular metabolism and virulence pathways. We also identified a variant of the major virulence factor, M1 protein, lacking 13 amino acids at the NH(2)-terminus in bacteria grown with plasma. The pronounced effect of plasma on protein expression, suggests this is an important adaptive mechanism with implications for S. pyogenes pathogenicity.
Johansson
Björn P
BP
Department of Clinical Sciences, Lund University, Lund, Sweden. Bjorn.Johansson@medkem.lu.se
Levander
Fredrik
F
von Pawel-Rammingen
Ulrich
U
Berggård
Tord
T
Björck
Lars
L
James
Peter
P
D000596:Amino Acids / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D001692:Biological Transport; D002853:Chromatography, Liquid; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D013058:Mass Spectrometry; D010949:Plasma / Q000382:microbiology*; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013297:Streptococcus pyogenes / Q000378:metabolism* / Q000472:pathogenicity; D015854:Up-Regulation; D014763:Viral Matrix Proteins / Q000378:metabolism; D014774:Virulence
D000596:Amino Acids; D001426:Bacterial Proteins; D011506:Proteins; D014763:Viral Matrix Proteins