Tickborne pathogen detection, Western Siberia, Russia.
Ixodes persulcatus (n = 125) and Dermacentor reticulatus (n = 84) ticks from Western Siberia, Russia, were tested for infection with Borrelia, Anaplasma/Ehrlichia, Bartonella, and Babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. I. persulcatus ticks were infected with Borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), Anaplasma phagocytophilum (2.4% +/- 1.4%), Ehrlichia muris (8.8% +/- 2.5%), and Bartonella spp. (37.6% +/- 4.3%). D. reticulatus ticks contained DNA of B. burgdorferi sensu lato (3.6% +/- 2.0%), Bartonella spp. (21.4% +/- 4.5%), and Babesia canis canis (3.6% +/- 2.0%). Borrelia garinii, Borrelia afzelii, and their mixed infections were observed among I. persulcatus, whereas B. garinii NT29 DNA was seen in samples from D. reticulatus. Among the I. persulcatus ticks studied, no Babesia spp. were observed, whereas B. canis canis was the single subspecies found in D. reticulatus.
Rar
Vera A
VA
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch--Russian Academy of Sciences, Lavrentyev's Avenue 8, 630090 Novosibirsk, Russia.
Fomenko
Natalia V
NV
Dobrotvorsky
Andrey K
AK
Livanova
Natalya N
NN
Rudakova
Svetlana A
SA
Fedorov
Evgeniy G
EG
Astanin
Vadim B
VB
Morozova
Olga V
OV
D041081:Anaplasma phagocytophilum / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D003870:Dermacentor / Q000382:microbiology / Q000469:parasitology; D004536:Ehrlichia / Q000235:genetics / Q000302:isolation & purification; D018884:Ixodes / Q000382:microbiology / Q000469:parasitology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA; D012800:Siberia / Q000453:epidemiology; D017282:Tick-Borne Diseases / Q000382:microbiology* / Q000469:parasitology*
D004269:DNA, Bacterial; D016054:DNA, Protozoan