Potential-modulated DNA cleavage by (N-salicylideneglycinato)copper(II) complex.
The interaction of aqua (N-salicylideneglycinato)copper(II) (Cu(salgly)2+) complex with calf thymus DNA has been investigated by cyclic voltammetry. Potential-modulated DNA cleavage in the presence of Cu(salgly)2+ complex was performed at a gold electrode in a thin layer cell. DNA can be efficiently cleaved by electrochemically reducing Cu(salgly)2+ complex to Cu(salgly)+ complex at -0.7 V (vs. Ag/AgCl). When the solution was aerated with a small flow of O2 during electrolysis, the extent of DNA cleavage was dramatically enhanced, and hydroxyl radical scavengers inhibited DNA cleavage. These results suggested that O2 and hydroxyl radical were involved in potential-modulated DNA cleavage reaction. The percentage of DNA cleavage was enhanced as the working potential was shifted to more negative values and the electrolysis time was increased. It was also dependent on the ratio of Cu(salgly)2+ complex to DNA concentration. The cleaved DNA fragments were separated by high performance liquid chromatography (HPLC). The experimental results indicated that the method for potential-modulated DNA cleavage by Cu(salgly)2+ complex was simple and efficient.
Yang
Zhou-Sheng
ZS
School of Chemistry and Materials Science, Anhui Normal University, Wuhu, PR China. yzhoushe@mail.ahnu.edu.cn
Wang
Yan-Ling
YL
Liu
Yun-Chun
YC
Zhao
Guang-Chao
GC
D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D016166:Free Radical Scavengers / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D012459:Salicylates / Q000737:chemistry*
D016166:Free Radical Scavengers; D012459:Salicylates; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D010100:Oxygen