title: A fluorescence-based assay for indoleamine 2,3-dioxygenase.
abstract: A rapid and sensitive fluorescence-based bioassay for determination of indoleamine 2,3-dioxygenase (IDO) activity has been developed. This assay relies on the quantification of the amount of kynurenine produced in the assay medium by fluorescence and complements the standard absorbance and high-performance liquid chromatography (HPLC) assay methods. The fluorescence method has limits of detection similar to those of the standard assay methods. Measured activities of IDO, including in the presence of tryptophan-based inhibitors, were in statistical agreement with the absorbance and HPLC assay methods. The fluorescence-based assay was also suitable for assessment of IDO inhibition by compounds that are incompatible with the absorbance method.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Matin
authors.forename: Isla M
authors.initials: IM
authors.lastname: Streete
authors.forename: Ian M
authors.initials: IM
authors.lastname: Jamie
authors.forename: Roger J W
authors.initials: RJ
authors.lastname: Truscott
authors.forename: Joanne F
authors.initials: JF
authors.lastname: Jamie
mesh_terms: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000592:standards; D007737:Kynurenine; D012015:Reference Standards; D012972:Sodium Hydroxide; D013050:Spectrometry, Fluorescence / Q000379:methods; D014238:Trichloroacetic Acid
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007737:Kynurenine; D012972:Sodium Hydroxide; D014238:Trichloroacetic Acid