HPLC screening of natural vitamin E from mediterranean plant biofactories--a basic tool for pilot-scale bioreactors production of alpha-tocopherol.
The study was performed in order to investigate a simple, efficient, reliable and rapid method of extracting and quantifying natural vitamin E for pressurized liquid extraction (PLE) as well as high-performance liquid chromatography (HPLC) analysis. Lyophilized Corylus avellana L. nut samples were powdered by high-speed milling with Waring blender for 40 s. alpha-Tocopherol was extracted from the nut tissue powder using dehydrated hexane fortified with 0.01% butylated hydroxytoluene (BHT, co-antioxidant). The rate of alpha-tocopherol accumulation showed differences among nut samples collected in different areas of Italy. Sarda Piccola nut biofactory contained higher amount (81.17 microg/g d.w) of alpha-tocopherol than other-local eleven Italian cultivar nuts. These results provide insight into the biofactory basis for alpha-tocopherol accumulation in hazelnut and give the suitable cultivar tissues to establish pilot-scale bioreactors production of natural bioactive vitamin E.
Sivakumar
G
G
BIOTEC-GEN, ENEA, Casaccia, Rome, Italy.
Bacchetta
L
L
Gatti
R
R
Zappa
G
G
D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009754:Nuts; D010944:Plants / Q000737:chemistry*; D014810:Vitamin E / Q000032:analysis* / Q000138:chemical synthesis*
D014810:Vitamin E