title: Using redox potential to detect microbial activities during clavulanic acid biosynthesis in Streptomyces clavuligerus.
abstract: Production of clavulanic acid (CA) by Streptomyces clavuligerus in a shake-flask culture increased from 92 to 180 mg l(-1) with an increased O2 transfer efficiency (0.039 --> 0.058 s(-1)), which maintained the redox potential values above -250 mV. Compared with traditional measures, such as dissolved O2 concentration and respiratory activity, the redox potential can easily be determined and correlates closely with CA production. It can therefore be used to monitor microbial activities during biosyntheses of secondary metabolites.
authors.affiliation: Energy & Resources Laboratories, Industrial Technology Research Institute, Hsinchu, 310, Taiwan.
authors.forename: Yun-Huin
authors.initials: YH
authors.lastname: Lin
authors.forename: Sz-Chwun John
authors.initials: SC
authors.lastname: Hwang
authors.forename: Jian Tong
authors.initials: JT
authors.lastname: Gong
authors.forename: Jane-Yii
authors.initials: JY
authors.lastname: Wu
authors.forename: Kuo-Cheng
authors.initials: KC
authors.lastname: Chen
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D019818:Clavulanic Acid / Q000096:biosynthesis*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D011048:Polarography / Q000379:methods*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D019818:Clavulanic Acid; D010100:Oxygen