Identification and functional analysis of genes required for desulfurization of alkyl dibenzothiophenes of Mycobacterium sp. G3.
Mycobacterium sp. G3 was reported as a dibenzothiophene (DBT)-degrading microorganism and the first strain to have the ability to degrade high-molecular-weight alkyl DBTs, such as 4,6-dibutyl DBT and 4,6-dipentyl DBT, by the C-S bond cleavage pathway. Three genes (mdsA, mdsB, and mdsC) for desulfurization, which form a cluster, were cloned from Mycobacterium sp. G3. The expression of each gene in Escherichia coli JM109 showed that MdsC oxidized DBT to DBT sulfone, MdsA transformed DBT sulfone into 2-(2'-hydroxyphenyl)benzene sulfinate (HPBS), and MdsB desulfinated HPBS into 2-hydroxybiphenyl (HBP), indicating that the gene products of mdsABC are functional in the recombinant. MdsC oxidized 4,6-dimethyl DBT, 4,6-diethyl DBT, 4,6-dipropyl DBT and 4,6-dibutyl DBT to each sulfone form, suggesting that MdsC covers a broad specificity for alkyl DBTs.
Nomura
Nobuhiko
N
Graduate School of Life and Environmental Science, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba City, Ibaraki 305-8572, Japan. nomunobu@sakura.cc.tsukuba.ac.jp
Takada
Masaki
M
Okada
Hideki
H
Shinohara
Yuko
Y
Nakajima-Kambe
Toshiaki
T
Nakahara
Tadaatsu
T
Uchiyama
Hiroo
H
D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D009161:Mycobacterium / Q000201:enzymology* / Q000235:genetics*; D013379:Substrate Specificity; D013455:Sulfur / Q000378:metabolism; D013876:Thiophenes / Q000378:metabolism*
D013876:Thiophenes; D013455:Sulfur; C016366:dibenzothiophene