title: Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology.
abstract: The currently accepted culture techniques for the detection of Legionella spp. in water samples (AS/NZS 3896:1998 and ISO 11731 standard methods) are slow and laborious, requiring from 7 to 14 days for a result. We describe a fully validated rapid confirmation technique that uses real-time PCR incorporating the intercalating dye SYTO9 for the direct identification of primary cultures, significantly decreasing turnaround time and allowing faster remedial action to be taken by the industry.
authors.affiliation: Australian Water Quality Centre, PMB 3, Salisbury, South Australia 5108, Australia. steven.giglio@sawater.com.au
authors.forename: Steven
authors.initials: S
authors.lastname: Giglio
authors.forename: Paul T
authors.initials: PT
authors.lastname: Monis
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Saint
mesh_terms: D007841:Latex Fixation Tests / Q000379:methods; D007875:Legionella / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D014871:Water Microbiology