title: [Study on the frequency of alpha-1,2-fucosyltransferase gene h4 allele (C35T) in Chinese population].
abstract: OBJECTIVE
  To investigate the h4 allele (C35T) frequency of alpha-1,2-fucosyltransferase gene in Chinese population.
  
  
  METHODS
  The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identifying C35T variant was established by using PCR to amplify a 125 bp FUT1 gene fragment, including C35T variant sequence, and the PCR product was digested by Hae III restriction enzyme. One hundred and fifty-eight random samples from Chinese blood donor were screened by PCR-RFLP.
  
  
  RESULTS
  Among 158 Chinese individuals with normal ABO and H blood group phenotypes, 8 and 83 were homozygous with 35T/T and 35C/C, respectively, while 67 were heterozygous with 35C/T. The allele frequencies were compatible with Hardy-Weinberg equilibrium.
  
  
  CONCLUSIONS
  The C35T substitution of FUT1 gene is not a mutation which gives rise to a non-functional h allele responsible for para-Bombay phenotype but a single nucleotide polymorphism in Chinese population.
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province, P. R. China.
authors.forename: Xian-guo
authors.initials: XG
authors.lastname: Xu
authors.forename: Xiao-zhen
authors.initials: XZ
authors.lastname: Hong
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Wu
authors.forename: Kai-rong
authors.initials: KR
authors.lastname: Ma
authors.forename: Qi-hua
authors.initials: QH
authors.lastname: Fu
authors.forename: Li-xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D000017:ABO Blood-Group System; D005647:Fucosyltransferases; D000097631:Galactoside 2-alpha-L-fucosyltransferase