Analysis of macamides in samples of Maca (Lepidium meyenii) by HPLC-UV-MS/MS.
The macamides are a distinct class of secondary metabolites that have so far been found only in Lepidium meyenii Walp. (Maca). Using HPLC-UV-MS/MS, the main macamides have been identified as n-benzylhexadecanamide, n-benzyl-(9Z)-octadecenamide, n-benzyl-(9Z, 12Z)-octadecadienamide, n-benzyl-(9Z, 12Z, 15Z)-octadecatrienamide and n-benzyloctadecanamide. The identities of n-benzyl-(9Z)-octadecenamide and n-benzyl-(9Z, 12Z)-octadecadienamide were confirmed by comparison of chromatographic and spectral properties with synthetic analogues. Total macamides have been quantified by HPLC-UV in plant material from different vendors using n-benzylhexadecanamide as an external standard. The amount of macamides in the dried plant material ranged from 0.0016 to 0.0123%.
McCollom
Megan M
MM
Tom's of Maine, Kennebunk, ME 04043, USA.
Villinski
Jacquelyn R
JR
McPhail
Kerry L
KL
Craker
Lyle E
LE
Gafner
Stefan
S
D000577:Amides / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D029686:Lepidium / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet
D000577:Amides