Environmental contamination before, during, and after dental treatment.
OBJECTIVE
To verify environmental contamination by collecting microorganisms from dental equipment surfaces before, during and after clinical procedures.


METHODS
Sterile swabs were used to collect the samples from dental-chair push buttons, 3-in-1 syringes, X-ray tubes, computer keys, doorknobs, and light handles before (P1), during (P2) and after (P3) clinical procedures, in the undergraduate clinic of the Dental School of Piracicaba, University of Campinas, Brazil. These samples were spread on BHI agar and incubated at 37 degrees C for 24 hours. Other dishes containing the same material were incubated at 37 degrees C in 10% CO2 for 48 hours. The resulting microorganisms were counted and classified using the Gram staining and biochemical tests. Microorganism counts among the periods and groups were analyzed by Kruskal-Wallis test (alpha=5%).


RESULTS
The most prevalent microorganisms were Viridans group streptococci, Sthaphylococcus epidermidis and Baccillus subtilis. Push buttons were the most contaminated (P< 0.05). Microorganisms were most prevalent during clinical procedures (P< 0.05). Microorganism counts were higher in P2 than in P1 and P3 (P< 0.05). Clinical activity caused an increase in the number of environmental microorganisms, where Viridans group streptococci were the most prevalent contaminant found on equipment surfaces.
Motta
Rogério Heládio Lopes
RH
Department of Physiologic Sciences, Dental School of Piracicaba, University of Campinas, Av. Limeira #901, Areião - CEP 13,414-903, Piracicaba, São Paulo, Brazil. rogeriomotta@fop.unicamp.br
Ramacciato
Juliana Cama
JC
Groppo
Francisco Carlos
FC
Pacheco
Aline de Barros Nóbrega Dias
Ade B
de Mattos-Filho
Thales Rocha
TR
D001412:Bacillus subtilis / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003729:Dental Care; D003762:Dental Instruments / Q000382:microbiology*; D004866:Equipment Contamination; D013212:Staphylococcus epidermidis / Q000302:isolation & purification; D018709:Statistics, Nonparametric; D013997:Time Factors; D034363:Viridans Streptococci / Q000302:isolation & purification