title: Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry.
abstract: MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequences of the PVK/CAP2b neuropeptides in the stable fly Stomoxys calcitrans and horn fly Haematobia irritans, insect pests of livestock. This peptidomic analysis of single neurohemal organ preparations allows the unambiguous assignment of internal Leu/Ile positions not distinguishable by previous mass spectrometric techniques. The sequences are as follows: Stoca-PVK/CAP2b-1, AGGASGLYAFPRVa; Stoca-PVK/CAP2b-2, NAKLYPVPRVa; and Haeir-PVK/CAP2b-1, AGGASGLYAFPRVa; Haeir-PVK/CAP2b-1, NAKLYPMPRVa. Both Stoca-PVK/CAP2b-1 and -2 stimulate Malpighian tubule fluid secretion in the stable fly, with EC50 values between 3 and 11 nM. The identification of these novel neuropeptides adds to our knowledge of the peptidomes of flies, and can aid in the development of neuropeptide-based control strategies of these insect pests.
authors.affiliation: Areawide Pest Management Research, Southern Plains Agricultural Research Center, USDA, 2881 F/B Road, College Station, TX 77845, USA. nachman@tamu.edu
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Nachman
authors.forename: William K
authors.initials: WK
authors.lastname: Russell
authors.forename: Geoffrey M
authors.initials: GM
authors.lastname: Coast
authors.forename: David H
authors.initials: DH
authors.lastname: Russell
authors.forename: J Allen
authors.initials: JA
authors.lastname: Miller
authors.forename: Reinhard
authors.initials: R
authors.lastname: Predel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D008297:Male; D008317:Malpighian Tubules / Q000187:drug effects / Q000378:metabolism; D009117:Muscidae / Q000737:chemistry; D009479:Neuropeptides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009490:Neurosecretory Systems / Q000737:chemistry; D009842:Oligopeptides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016415:Sequence Alignment; D013268:Stimulation, Chemical
chemical_list: D009479:Neuropeptides; D009842:Oligopeptides; C093061:periviscerokinin