title: Nucleotide sequence of Blackberry yellow vein associated virus, a novel member of the Closteroviridae.
abstract: The complete nucleotide sequence of a novel member of the genus Crinivirus (family Closteroviridae), isolated from blackberry and tentatively named Blackberry yellow vein associated virus, was determined. The virus possesses a bipartite genome. RNA 1 is 7,801 nucleotides in length and papain-like protease, methyltransferase, RNA helicase and RNA-dependent RNA polymerase motifs have been identified in the proteins coded for by this molecule. The polymerase is probably expressed via a +1 ribosomal frameshift, a common feature among members of the Closteroviridae. RNA 2 is 7,917 nucleotides long and encodes nine open reading frames, similar in size and position to orthologous genes of other criniviruses with the exception of a second hydrophobic peptide found near the 5' terminus of the molecule. Phylogenetic analysis revealed a close relationship between Blackberry yellow vein associated virus and Beet pseudo yellows virus, another crinivirus that infects small fruit crops.
authors.affiliation: Department of Botany and Plant Pathology & Center for Gene Research and Biotechnology, Oregon State University, Corvallis, 97331, USA. yannis@orst.edu
authors.forename: Ioannis E
authors.initials: IE
authors.lastname: Tzanetakis
authors.forename: James
authors.initials: J
authors.lastname: Susaimuthu
authors.forename: Rose C
authors.initials: RC
authors.lastname: Gergerich
authors.forename: Robert R
authors.initials: RR
authors.lastname: Martin
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D029383:Crinivirus / Q000235:genetics* / Q000302:isolation & purification; D005638:Fruit / Q000821:virology; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral; D027824:Rosaceae / Q000821:virology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins