Roles of the Foxj1 and Inv genes in the left-right determination of internal organs in mice.
In Foxj1 knockout mice, half show situs solitus while the other half show situs inversus, which means a random determination of the left-right axis. In contrast, the inv mutant mice show a mirror-image configuration of the internal organs, which means a reversal of the left-right axis. Although these two mutant mice have primary cilia on the nodal cells, their phenotypes are different in laterality determination. We thus made Foxj1/inv double mutant mice and analyzed their phenotype. We found the phenotypes of Foxj1/inv double mutant mice to be more similar to those of the Foxj1 mutant mice than those of the inv mutant mice. We also found right pulmonary isomerism to be a major phenotype of the Foxj1 mutant mice and the Foxj1/inv double mutant mice, which is likely due to the absence of the Pitx2 expression at both lateral plate mesoderms. These results indicate that a random signal of laterality (Foxj1) is dominant over the reversal signal of laterality (Inv).
Tamakoshi
Tomoki
T
Department of Biochemistry, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, Japan.
Itakura
Tatsuo
T
Chandra
Abhishek
A
Uezato
Tadayoshi
T
Yang
Zhi
Z
Xue
Xiao-Dong
XD
Wang
Bo
B
Hackett
Brian P
BP
Yokoyama
Tatsuhiko
T
Miura
Naoyuki
N
D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D007839:Functional Laterality / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012857:Situs Inversus / Q000196:embryology / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism*
C481963:FOXJ1 protein, mouse; D051858:Forkhead Transcription Factors; C491947:Invs protein, mouse; D014157:Transcription Factors