Evaluation of biocompatibility of metallic dental materials in cell culture model.
Biocompatibility of metals for dental use was tested using a three-dimensional model consisting of oral keratinocytes cultured on de-epidermised sub-mucosa. The toxicity of orthodontic metallic wire and soldering material was assessed through parameters such as the morphology and growth rate of the keratinocytes, as well as by classical histology, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The sharp composition of metallic wires and soldering materials was assessed by Auger Electron Spectroscopy (AES). The results of our experiment showed that the new model revealed inhibition of keratinocyte growth and stratification near soldering material, whereas mucosal cells were able to grow and layer out on dental wire. It is concluded that this experimental model, which simulates the oral environment, is useful for studying the effects of materials for dental use for its sensibility and reproducibility. Moreover it can provide morpho-functional information which cannot be achieved by traditional methods.
Pianigiani
E
E
Dip. di Dermatologia, Università di Siena, Policlinico Le Scotte, Siena, Italy. pianigianie@unisi.it
Andreassi
A
A
Lorenzini
G
G
Alessandrini
C
C
Fimiani
M
M
Atrei
A
A
Fonzi
L
L
Giorgetti
R
R
Sestini
S
S
D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003722:Dental Alloys / Q000633:toxicity*; D012994:Dental Soldering / Q000009:adverse effects*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D008422:Materials Testing; D008851:Micropore Filters; D008854:Microscopy, Electron; D009061:Mouth Mucosa; D009969:Orthodontic Wires / Q000009:adverse effects*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods
D003722:Dental Alloys