[Study on the safety and immunogenicity of enterotoxigenic Escherichia coli recombinant active vaccine].
OBJECTIVE
To study the safety, immunogenicity on the enterotoxige Escherichia coli (E. coli) recombinant active vaccine FE3 and FE16.


METHODS
Toxicity and immunogenicity of the vaccine were determined by experiments on enterotoxigenic E. coli toxicity and immunological experiments on rabbits and mice.


RESULTS
The results of an toxicological experiments were negative. The agglutination titer of antibodies against the S. flexneri 2a and enterotoxigenic E. coli plamid antigen were all higher than 1:640 and 1:1280 in the sera of rabbits. IgG in the serum went up remarkably, while sIgA against CFA/I was also decteted in the dejecta of mice immunized with active bacteria either orogastrically or intranasally. Simultaneously, sIgA was not detected in the dejecta of mice immunized with inactive bacteria either orogastrically or intranasally.


CONCLUSIONS
The enterotoxigenic E. coli recombinant active vaccine showed good safety and immunogenicity, inducing both humoral and mucosal immunity in mice.
Wang
Zhi-bin
ZB
Guangzhou Institute of Military Medicine, Guangzhou 510507, China.
Zeng
Nian-hua
NH
Jiang
Pu-lin
PL
Li
Shu-qin
SQ
Zhang
Zhao-shan
ZS
D000281:Administration, Intranasal; D000284:Administration, Oral; D000371:Agglutination / Q000276:immunology; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology*; D064420:Drug-Related Side Effects and Adverse Reactions / Q000276:immunology*; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology*; D005260:Female; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D011817:Rabbits; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology
D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic