title: Differential expression of manganese superoxide dismutase sequence variants in near isogenic lines of wheat during cold acclimation.
abstract: Numerous sequence variants of wheat (Triticum aestivum L.) manganese superoxide dismutase (MnSOD) genes have been found. Quantitative real-time PCR was used to measure the expression levels of three MnSOD genes distinguished by a variable amino acid, and three genes distinguished by sequence variation in the 3' untranslated region (3' UTR), in wheat plants grown at 20 degrees C and cold-acclimated for 1-4 weeks at 2 degrees C. The amino acid variants did not differ significantly in expression levels, however, differential expression of genes differing in the 3' UTR was observed. Diploid wheat-related species also carried sequence variants of MnSOD, with differing levels of expression, suggesting diversification of the MnSOD gene family occurred prior to the polyploidization events of hexaploid wheat.
authors.affiliation: Department of Crop and Soil Sciences, 210 Johnson Hall, Washington State University, Pullman, WA 99164-6420, USA.
authors.forename: Kwang-Hyun
authors.initials: KH
authors.lastname: Baek
authors.forename: Daniel Z
authors.initials: DZ
authors.lastname: Skinner
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000064:Acclimatization; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D003080:Cold Temperature; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014908:Triticum / Q000201:enzymology* / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D000596:Amino Acids; D012333:RNA, Messenger; D013482:Superoxide Dismutase