title: The anticarcinogenic effect of propolis in human lymphocytes culture.
abstract: The in vitro anticarcinogenic potential of propolis in human lymphocytes was investigated. Blood samples were obtained from ten healthy males, non-smoking volunteers, which were incubated and exposed to increasing concentrations of propolis (0.01, 0.05, 0.1, 0.2, 0.5, 0.7 and 1.0 ml). The mean micronucleus rates were 1.47 +/- 0.38 - 4.02 +/- 0.64. Mitotic index rates were between 19.45 +/- 2.22 and 0.28 +/- 0.33. The differences between the control and exposed cells were statistically significant (p < or = 0.05). We conclude that exposure to different concentrations of propolis cannot produce a carcinogenic effect in peripheral human lymphocytes in vitro. However, increasing micronucleus (MN) rates showed that propolis could have a carcinogenic effect in high concentrations. Also chemical analysis of propolis sample was evaluated by GC/MS. Propolis sample mainly contains flavonoids, fatty and aromatic acids and their esters.
authors.affiliation: Department of Genetic, Erciyes University Medical Faculty, Kayseri, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Ozkul
authors.forename: S
authors.initials: S
authors.lastname: Silici
authors.forename: E
authors.initials: E
authors.lastname: Eroğlu
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology; D008297:Male; D015162:Micronucleus Tests; D008940:Mitotic Index; D011429:Propolis / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D016588:Anticarcinogenic Agents; D002273:Carcinogens; D011429:Propolis