title: Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region.
abstract: The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome.
authors.affiliation: Department of Plant Sciences, Arizona Genomics Institute, University of Arizona, Tucson, AZ 85721, USA.
authors.forename: Meizhong
authors.initials: M
authors.lastname: Luo
authors.forename: Yeisoo
authors.initials: Y
authors.lastname: Yu
authors.forename: Hyeran
authors.initials: H
authors.lastname: Kim
authors.forename: Dave
authors.initials: D
authors.lastname: Kudrna
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Itoh
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Agate
authors.forename: Esther
authors.initials: E
authors.lastname: Melamed
authors.forename: José L
authors.initials: JL
authors.lastname: Goicoechea
authors.forename: Jayson
authors.initials: J
authors.lastname: Talag
authors.forename: Christopher
authors.initials: C
authors.lastname: Mueller
authors.forename: Wenming
authors.initials: W
authors.lastname: Wang
authors.forename: Jennifer
authors.initials: J
authors.lastname: Currie
authors.forename: Nicholas B
authors.initials: NB
authors.lastname: Sisneros
authors.forename: Rod A
authors.initials: RA
authors.lastname: Wing
authors.forename: Arthur P
authors.initials: AP
authors.lastname: Arnold
mesh_terms: D000818:Animals; D001483:Base Sequence; D001522:Behavior, Animal / Q000502:physiology; D002645:Chickens / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D046369:Finches / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D011944:Receptors, Androgen / Q000235:genetics*
chemical_list: D011944:Receptors, Androgen