GATA-3 expression in the development of anterior chamber associated immune deviation.
BACKGROUND
Anterior chamber associated immune deviation (ACAID) is characterized by a Th2 cell response. GATA-3 has been shown to be necessary for the activation of Th2 cells. This study was designed to examine the expression of GATA-3 in the development of ACAID.


METHODS
ACAID was induced by injection of 50 microg interphotoreceptor retinoid binding protein (IRBP) into the anterior chamber (AC) of Wistar rats. Delayed-type hypersensitivity (DTH) was evaluated on day 3, 7, 14, 21, 28 after IRBP inoculation. GATA-3 expression was detected using immunohistochemical staining. The expression of GATA-3 mRNA at different time points after AC injection of IRBP was assayed by reverse transcriptase polymerase chain reaction (RT-PCR).


RESULTS
A significant DTH reaction was observed in Wistar rats on day 3 and 5 after IRBP inoculation. The DTH reaction was decreased 7 days after IRBP inoculation. GATA-3 expression was weak at both mRNA and protein levels in the normal spleen, but was significantly increased on day 5, 7, 14, and 21 after AC injection of IRBP.


CONCLUSIONS
The expression of GATA-3 is increased during ACAID, suggesting that GATA-3 may be involved in the development of ACAID.
Fu
Tao
T
Uveitis Study Center, Sun Yat-sen University, Guangzhou 510060, China.
Yang
Pei-zeng
PZ
Huang
Xiang-kun
XK
Huang
Qiang
Q
Zhou
Hong-yan
HY
Li
Bing
B
Zhong
Hua-hong
HH
Chen
Xuan
X
D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D005136:Eye Proteins / Q000276:immunology; D005260:Female; D050990:GATA3 Transcription Factor / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012177:Retinol-Binding Proteins / Q000276:immunology; D013154:Spleen / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology*; D000099214:Retinol-Binding Proteins, Interstitial
D005136:Eye Proteins; D050990:GATA3 Transcription Factor; D012333:RNA, Messenger; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial