[Establishing a method to detect the DNA damage of N-ras gene].
OBJECTIVE
Applying RDPCR to detect the DNA damage of N-ras gene in human.


METHODS
Single-primer PCR was used to prepare single strand (ss) probes of extron 1 of N-ras gene in human. The genomic DNA was digested completely by restriction endonuclease, then amplified by RDPCR and detected by Southern hybridization with the probe.


RESULTS
The ss probes were successfully prepared by single-primer PCR. The hybridized bands were clearly seen in the expected migration positions.


CONCLUSIONS
The result shows that the method to detect the damaged position of N-ras gene has been established, which would be helpful to further studies on chemical carcinogenesis and on the prevention of tumor.
Rao
Chao-Long
CL
Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Zhang
Zun-Zhen
ZZ
Heng
Zheng-Chang
ZC
D004249:DNA Damage; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000517:prevention & control; D016133:Polymerase Chain Reaction / Q000379:methods