title: Mammalian embryonic cerebrospinal fluid proteome has greater apolipoprotein and enzyme pattern complexity than the avian proteome.
abstract: During early stages of embryo development, the brain cavity is filled with Embryonic Cerebro-Spinal Fluid, which has an essential role in the survival, proliferation and neurogenesis of the neuroectodermal stem cells. We identified and analyzed the proteome of Embryonic Cerebro-Spinal Fluid from rat embryos (Rattus norvegicus), which includes proteins involved in the regulation of Central Nervous System development. The comparison between mammalian and avian Embryonic Cerebro-Spinal Fluid proteomes reveals great similarity, but also greater complexity in some protein groups. The pattern of apolipoproteins and enzymes in CSF is more complex in the mammals than in birds. This difference may underlie the greater neural complexity and synaptic plasticity found in mammals. Fourteen Embryonic Cerebro-Spinal Fluid gene products were previously identified in adult human Cerebro-Spinal Fluid proteome, and interestingly they are altered in patients with neurodegenerative diseases and/or neurological disorders. Understanding these molecules and the mechanisms they control during embryonic neurogenesis may contribute to our understanding of Central Nervous System development and evolution, and these human diseases.
authors.affiliation: Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Catalonia, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: Parada
authors.forename: Angel
authors.initials: A
authors.lastname: Gato
authors.forename: David
authors.initials: D
authors.lastname: Bueno
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000134:cerebrospinal fluid* / Q000737:chemistry*; D001717:Birds; D001921:Brain / Q000196:embryology*; D049109:Cell Proliferation; D002555:Cerebrospinal Fluid / Q000737:chemistry*; D002645:Chickens; D015180:Electrophoresis, Gel, Two-Dimensional; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008322:Mammals; D013058:Mass Spectrometry; D019636:Neurodegenerative Diseases / Q000473:pathology; D009474:Neurons / Q000378:metabolism; D011506:Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D051381:Rats; D013997:Time Factors
chemical_list: D001053:Apolipoproteins; D011506:Proteins; D020543:Proteome