Reduction of nonspecific binding proteins to self-assembled monolayer on gold surface.
We developed a gold coated glass chip bearing a poly(ethyleneglycol) (PEG) type compound as hydrophilic spacer for surface plasmon resonance studies, which enabled adequate estimation of K(d) value between FK506 and FKBP12 not only using purified FKBP12 (K(d)=22 nM) but also using Escherichia coli lysate expressing FKBP12 (K(d)=15 nM). These results indicated effectiveness of the PEG spacer for reduction of nonspecific interactions. Chemical stability and simple surface-structure of the novel chip are also attractive.
Furuya
Minoru
M
Chemistry Department, Reverse Proteomics Research Institute Co. Ltd, 2-6-7 Kazusa-Kamatari, Chiba 292-0818, Japan.
Haramura
Masayuki
M
Tanaka
Akito
A
D000818:Animals; D001921:Brain; D004926:Escherichia coli / Q000737:chemistry; D006046:Gold; D051381:Rats; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D016559:Tacrolimus / Q000737:chemistry*; D022061:Tacrolimus Binding Protein 1A / Q000737:chemistry*
D006046:Gold; D022061:Tacrolimus Binding Protein 1A; D016559:Tacrolimus