Multiplex PCR assay that identifies the major lipooligosaccharide serotype expressed by Moraxella catarrhalis clinical isolates.
A heterologous cluster of glycosyltransferase genes was identified in the three Moraxella catarrhalis LOS serotype strains. Multiple PCR primers designed to this region amplified products that differentiate between the serotypes more rapidly and efficiently than previously described serological analyses. This assay will be valuable for clinical and research-based studies.
Edwards
Katie J
KJ
Department of Microbiology, University at Buffalo, Biomedical Research Bldg. Rm. 143, 3435 Main Street, Buffalo, NY 14214, USA.
Schwingel
Johanna M
JM
Datta
Anup K
AK
Campagnari
Anthony A
AA
D000328:Adult; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D016695:Glycosyltransferases / Q000235:genetics; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism*; D001936:Moraxella catarrhalis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D045828:Moraxellaceae Infections / Q000382:microbiology; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping
D004269:DNA, Bacterial; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides; D016695:Glycosyltransferases