[Antiviral effect of Folium Isatidis on herpes simplex virus type I].
OBJECTIVE
To evaluate the antiviral effect of different chemical fractions isolated from Folium Isatidis on herpes simplex virus type I (HSV- I).


METHODS
Anti- HSV- I effects of Folium Isatidis were investigated in Hep-2 cell by adopting MTT colorimetric assay and observing cytopathic effect (CPE). Treatment index (TI) was used to evaluate the antiviral activity of different chemical fractions from Folium Isatidis. The antiviral mechanism of Folium Isatidis was investigated by changing the different ways of drug administration.


RESULTS
The fractions II approximately V from Folium Isatidis inactivate HSV- I directly. None of the chemical fractions had antiviral effects of adsorption-blocking. The chemical fractions except fraction III and fraction V inhibited the biological synthesis of HSV- I. The fraction IV significantly reduce death rate of mice infected with HSV- I.


CONCLUSIONS
The fraction IV from Folium Isatidis has powerful anti-HSV-lI effect in vitro and in vivo.
Fang
Jian-guo
JG
Department of Pharmacy, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 43003, China. fjg3560@sina.com
Hu
Ya
Y
Tang
Jie
J
Wang
Wen-qing
WQ
Yang
Zhan-qiu
ZQ
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000633:toxicity; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D006801:Humans; D031219:Isatis / Q000737:chemistry*; D007822:Laryngeal Neoplasms / Q000473:pathology; D008297:Male; D051379:Mice; D018515:Plant Leaves / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal