title: Induction of phenolic compounds in Hypericum perforatum L. cells by Colletotrichum gloeosporioides elicitation.
abstract: Changes in phenolic metabolism after elicitation with Colletotrichum gloeosporioides (CG) has been studied in Hypericum perforatum L. (HP) cell suspension cultures. Soluble phenolics were analysed by HPLC-DAD and HPLC-DAD-MS/MS. HP cultures elicited with the CG elicitor showed a significant increase in xanthone accumulation. Xanthone accumulation increased twelve fold when the cells were primed with methyl-jasmonate (MeJ) or salicylic acid (SA), before elicitation. HP cultures exposed only to MeJ produced a set of flavonoids, the flavones which represent a substantial part (approx. 40%) of the total flavonoids accumulated in these cells. The possible importance of xanthones as a component of defence mechanism of HP against biotic stress is discussed.
authors.affiliation: University of Minho, Department of Biology, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Luis F R
authors.initials: LF
authors.lastname: Conceição
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: Rui M
authors.initials: RM
authors.lastname: Tavares
authors.forename: Alberto C P
authors.initials: AC
authors.lastname: Dias
mesh_terms: D000085:Acetates / Q000494:pharmacology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D020231:Colletotrichum / Q000737:chemistry / Q000378:metabolism*; D003517:Cyclopentanes / Q000494:pharmacology; D020902:Hypericum / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D054883:Oxylipins; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D020156:Salicylic Acid / Q000494:pharmacology; D013045:Species Specificity; D044004:Xanthones / Q000737:chemistry / Q000378:metabolism
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010636:Phenols; D044004:Xanthones; C072239:methyl jasmonate; C009689:xanthone; D020156:Salicylic Acid