[Fusion vaccine therapy by IL-2-gene-transduced dendritic cells and tumor cells].
We evaluated the usefulness of fusion vaccine prepared from IL-2-gene-transduced splenic dendritic cells (DCs) and fibrosarcoma tumor cells (QRsP) in treating of lung metastasis. The IL-2 or LacZ gene was transferred into spleen-derived DCs using an adenoviral vector. Irradiated QRsP tumor cells were fused with IL-2 gene transduced DCs (fusion/IL-2) or LacZ gene transduced DCs (fusion/LacZ) by polyethyleneglycol. These fusion cells expressed major histocompatibility complex (MHC) class I and MHC class II, CD86, CD11c and CD8alpha. IFN-gamma and cytotoxic T lymphocyte (CTL) activity of splenic lymphocytes in mice vaccinated with fusion cells increased significantly as compared with those of DC or tumor cells vaccinated mice. CTL levels in fusion/IL-2-vaccinated mice were higher than that in fusion/LacZ-vaccinated mice. The number of lung metastasis in the fusion/IL-2 or fusion/LacZ-vaccineatd mice was significantly lower than that in mice vaccinated with DCs, tumor or PBS. The introduction of the IL-2 gene into fusion cells produced more potent therapeutic effects. Our results suggest that the fusion cells prepared from IL-2 gene transduced spleen derived DCs and tumor cells have the ability to induce therapeutic effect against lung metastasis.
Ogawa
Fujio
F
Dept. of Surgery, Teikyo University School of Medicine.
Iinuma
Hisae
H
Iwasaki
Kota
K
Tamura
Junko
J
Kumagai
Harumi
H
Inaba
Tsuyoshi
T
Fukushima
Ryouji
R
Kota
Okinaga
O
D000818:Animals; D051940:B7-2 Antigen / Q000032:analysis; D039521:CD11c Antigen; D016827:CD8 Antigens / Q000032:analysis; D002459:Cell Fusion; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005354:Fibrosarcoma / Q000473:pathology; D016233:Immunotherapy, Active; D007376:Interleukin-2 / Q000235:genetics*; D007763:Lac Operon; D008175:Lung Neoplasms / Q000556:secondary; D008285:Major Histocompatibility Complex; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D013154:Spleen / Q000166:cytology; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured; D014612:Vaccines / Q000276:immunology
D051940:B7-2 Antigen; D039521:CD11c Antigen; D016827:CD8 Antigens; C431698:CD8alpha antigen; D007376:Interleukin-2; D014612:Vaccines