Tethered bilayer lipid membranes based on monolayers of thiolipids mixed with a complementary dilution molecule. 1. Incorporation of channel peptides.
Tethered bilayer lipid membranes (tBLMs) are described based on the self-assembly of a monolayer on template stripped gold of an archea analogue thiolipid, 2,3-di-o-phytanyl-sn-glycerol-1-tetraethylene glycol-d,l-alpha-lipoic acid ester lipid (DPTL), and a newly designed dilution molecule, tetraethylene glycol-d,l-alpha-lipoic acid ester (TEGL). The tBLM is completed by fusion of liposomes made from a mixture of diphytanoylphosphatidyl choline (DPhyPC), cholesterol, and 1,2-diphytanoyl-sn-glycero-3-phosphate (DPhyPG) in a molar ratio of 6:3:1. Melittin and gramicidin are incorporated into these tBLMs as shown by surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS) studies. Ionic conductivity at 0 V vs Ag|AgCl, 3 M KCl, measured by EIS measurements are comparable to the results obtained by other research groups. Admittance plots as a function of potential are discussed on a qualitative basis in terms of the kinetics of ion transport through the channels.
He
Lizhong
L
Max Planck Institute for Polymer Research, Mainz, Germany.
Robertson
Joseph W F
JW
Li
Jing
J
Kärcher
Iris
I
Schiller
Stefan M
SM
Knoll
Wolfgang
W
Naumann
Renate
R
D006046:Gold / Q000737:chemistry; D006096:Gramicidin; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes; D020349:Surface Plasmon Resonance; D008063:Thioctic Acid
D008051:Lipid Bilayers; D008081:Liposomes; D006096:Gramicidin; D008063:Thioctic Acid; D006046:Gold