Clostridium asparagiforme sp. nov., isolated from a human faecal sample.
An obligatory anaerobic, Gram-positive, rod-shaped organism was isolated from faeces of a healthy human donor. It was characterized using biochemical, phenotypic and molecular taxonomic methods. The organism produced acetate, lactate, and ethanol as the major products of glucose fermentation. The G + C content was 53 mol%. Based on comparative 16S rRNA gene sequencing, the unidentified bacterium is a member of the Clostridium subphylum of the Gram-positive bacteria, and most closely related to species of the Clostridium coccoides cluster (rRNA cluster XIVa) [M.D. Collins et al., The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations, Int. J. Syst. Bacteriol. 44 (1994) 812-826]. Clostridium bolteae and Clostridium clostridioforme were identified as the most closely related described species. A 16S rRNA sequence divergence value of > 3% suggested that the isolate represents a new species. This was also supported by the gyrase-encoding gyrB gene sequences. Based on these findings, we propose the novel bacterium from human faeces to be classified as a new species, Clostridium asparagiforme. The type strain of C. asparagiforme is N6 (DSM 15981 and CCUG 48471).
Mohan
Ruchika
R
Department of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany.
Namsolleck
Pawel
P
Lawson
Paul A
PA
Osterhoff
Martin
M
Collins
Matthew D
MD
Alpert
Carl-Alfred
CA
Blaut
Michael
M
D019342:Acetic Acid / Q000378:metabolism; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001482:Base Composition; D003013:Clostridium / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D003015:Clostridium Infections / Q000382:microbiology; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D000431:Ethanol / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA / Q000235:genetics; D005840:Gentian Violet; D005947:Glucose / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010619:Phenazines; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D000431:Ethanol; D027081:DNA Gyrase; D005947:Glucose; D005840:Gentian Violet; D019342:Acetic Acid