title: COL1A2 (type I collagen) polymorphisms in the Colorado Indians of Ecuador.
abstract: BACKGROUND
  EcoRI, MspI and RsaI restriction fragment length polymorphisms (RFLPs) of the COL1A2 (type I collagen) gene are proving to be extremely informative markers for describing human populations; therefore they hold considerable potential for anthropogenetic research.
  
  
  OBJECTIVE
  The objective of this study was to characterize at the DNA level the Colorado Indians from Ecuador, for whom only blood group frequency information is available, and to investigate their relationships with the Cayapa-another Ecuadoran Native American group belonging to the same linguistic affiliation-and other world populations.
  
  
  METHODS
  Colorado Indians (n = 80) were analysed for the three anthropologically informative RFLPs of the COL1A2 gene. To better define the genetic relationship between this group and other populations, principal component analysis (PCA) was performed and genetic distances were estimated. Population genetic structure was tested through analysis of molecular variance (AMOVA) by comparing haplotype frequencies.
  
  
  RESULTS
  COL1A2 allele and haplotype frequencies showed a certain degree of heterogeneity between the two Chibchan populations of Ecuador. The AMOVA test detected a significant level of differentiation (Fst = 0.034, p = 0.0049) between Colorado and Cayapa Indians. PC and genetic distance analyses showed a clear-cut separation between African and non-African populations; within the latter, the two Native American groups were differentiated from each other.
  
  
  CONCLUSIONS
  The present findings suggest the presence of a low level of genetic relatedness between the Colorado and the Cayapa, despite their supposed common ethnogenesis. This confirms what has been inferred from other genetic data about the high degree of heterogeneity among Native Americans, even within the same linguistic branch, thus supporting the existence of genetic sub-structure within the central and southern American populations.
authors.affiliation: Dipartimento di Biologia, Università di Roma Tor Vergata, Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Babalini
authors.forename: T
authors.initials: T
authors.lastname: Tarsi
authors.forename: C
authors.initials: C
authors.lastname: Martínez-Labarga
authors.forename: G
authors.initials: G
authors.lastname: Scano
authors.forename: G
authors.initials: G
authors.lastname: Pepe
authors.forename: G F
authors.initials: GF
authors.lastname: De Stefano
authors.forename: O
authors.initials: O
authors.lastname: Rickards
mesh_terms: D000483:Alleles; D000704:Analysis of Variance; D001483:Base Sequence; D003094:Collagen / Q000235:genetics*; D024042:Collagen Type I; D004247:DNA / Q000235:genetics; D004484:Ecuador; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D007199:Indians, South American / Q000235:genetics*; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D025341:Principal Component Analysis
chemical_list: D024042:Collagen Type I; D003094:Collagen; D004247:DNA