[Protective effects of extract of Ginkgo biloba against ethanol-induced oxidative injury in rat testes].
OBJECTIVE
This study was designed to investigate whether EGB protect against ethanol-induced oxidative injury in rat testes.


METHODS
The rats were pretreated with EGB (4.8,9.6 mg/100g bw per day) before 30% ethanol administration (2.37g/kg bw by gastric incubation). Ninety days later, the rats were killed in order to measure the activities of Superoxide Dismutase (SOD), Glutathione Reductase (GST), Glutathione Peroxidase (GSH-Px), Catalase (CAT) and the contents of Glutathione (GSH), Reactive Oxygen Species (ROS), Malondialdehyde (MDA)in rat testes. The microsomal fractions were obtained by the method of differential centrifugation, and HO-1 (Heme Oxygenase-1) activity in testicular microsomal was measured. To examine the expression of HO-1 mRNA by reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
Compared with ethanol group, ROS, and MDA contents significantly decreased in EGB group testes, respectively (P < 0.05). GST, GSH-Px, CAT, SOD activities, and GSH content in EGB group testes elevated markedly (P <0.05). EGB could enhance HO-1 mRNA expression remarkably.


CONCLUSIONS
It is suggested that EGB as a preventive antioxidant can protect against ethanol-induced testicular injury, which may be associated with HO-1 activity enhancement, free radicals elimination, lipid per-oxidation reaction inhibition.
Li
Ke
K
Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Yao
Ping
P
Zhou
Shao-Liang
SL
Song
Fang-Fang
FF
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D020441:Ginkgo biloba / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D020011:Protective Agents / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000431:Ethanol; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione