The expression of N-cadherin, fibronectin during chondrogenic differentiation of MSC induced by TGF-beta(1).
OBJECTIVE
To investigate the role of N-cadherin and fibronectin during chondrogenesis.


METHODS
Immunohistochemical method and autibody induced changes of aggregation of cells were used to assay the expressions of N-cadherin and fibronectin during cell differentiation.


RESULTS
The N-cadherin was present in the area of the cell nodular area in the 24 hours group after adding chondrogenic revulsant, then there was a down-regulating trend. Fibronectin was expressed in 48 and 72 hours groups after adding chondrogenic revulsant, and showed to be negative afterward. The antibody against fibronectin or N-cadherin could inhibit the formation of cellular nodule markedly.


CONCLUSIONS
Cell adhesion factors play an important role during cell differentiation. TGF-beta(1) stimulates chondrogenesis via transition from an initial N-cadherin-contributing stage to a succedent fibronectin-contributing stage during the process of chondrogenesis in MSCs. Further study is needed to evaluate whether or not it can promote chondrogenesis by transfecting cDNA of CAMs to MSCs.
Li
Wen-Ge
WG
Department of Orthopaedics, Peking University Zhenzhen Hospital, Shenzhen 518036, China.
Xu
Xin-Xiang
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