title: Cryopreservation of economically valuable marine micro-algae in the classes Bacillariophyceae, Chlorophyceae, Cyanophyceae, Dinophyceae, Haptophyceae, Prasinophyceae, and Rhodophyceae.
abstract: The ability to routinely cryopreserve micro-algal species reduces costs associated with maintaining large culture collections and reduces the risks of losing particular strains or species through contamination and genetic drift. Cryopreservation is also a useful adjunct in aquaculture hatcheries for strains of micro-algae where the nutritional status may change as a result of continuous sub-culture. In this study, cryopreservation of isolates from seven micro-algal classes was investigated. Successful candidates included the marine dinoflagellates Amphidinium carterae, Amphidinium trulla, and Gymnodinium simplex, and the haptophytes Chrysochromulina simplex, Prymnesium parvum, Prymnesium parvum f. patelliferum, Isochrysis galbana, and Pavlova lutheri. Also successfully cryopreserved were the planktonic diatoms Chaetoceros calcitrans, Chaetoceros muelleri, Chaetoceros sp., and the benthic Nitzschia ovalis, the chlorophyte Chlamydomonas coccoides, the rhodophyte Porphyridium purpureum, the prasinophytes Tetraselmis chuii, and Tetraselmis suecica, and the cyanophytes Raphidiopsis sp., and Aphanizomenon flos-aquae. All species were successfully cryopreserved using 15% Me2SO.
authors.affiliation: Cawthron Institute, Private Bag 2, Nelson, New Zealand. Lesley.rhodes@cawthorn.org.nz
authors.forename: Lesley
authors.initials: L
authors.lastname: Rhodes
authors.forename: John
authors.initials: J
authors.lastname: Smith
authors.forename: Robin
authors.initials: R
authors.lastname: Tervit
authors.forename: Rodney
authors.initials: R
authors.lastname: Roberts
authors.forename: Janet
authors.initials: J
authors.lastname: Adamson
authors.forename: Serean
authors.initials: S
authors.lastname: Adams
authors.forename: Marie
authors.initials: M
authors.lastname: Decker
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D000458:Cyanobacteria / Q000502:physiology*; D004121:Dimethyl Sulfoxide; D056890:Eukaryota / Q000145:classification* / Q000502:physiology*; D013045:Species Specificity
chemical_list: D004121:Dimethyl Sulfoxide