Activation of a development-specific gene, dofA, by FruA, an essential transcription factor for development of Myxococcus xanthus.
FruA is an essential transcription factor for Myxococcus xanthus development. The expression of tps and dofA genes is fruA dependent. In this study, we show by gel shift and footprint assays with the C-terminal DNA-binding domain of FruA and by a lacZ fusion assay that FruA may directly activate dofA expression during development.
Ueki
Toshiyuki
T
Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA.
Inouye
Sumiko
S
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D018983:DNA Footprinting; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000502:physiology; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D016941:Myxococcus xanthus / Q000235:genetics* / Q000254:growth & development; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C104848:FruA protein, Myxococcus xanthus; D014157:Transcription Factors; D001616:beta-Galactosidase