title: Differentiation and protease production in Micromonospora echinospora (ATCC 15837).
abstract: Micromonospora echinospora differentiates in both submerged and surface cultures producing abundant dark spores after a period of vegetative mycelial growth. In submerged batch cultures, under either carbon or nitrogen limiting conditions, protease activity was found to coincide with sporulation indicating a relationship between proteolytic activity and differentiation in this organism. Further evidence for this link was provided from surface grown cultures wherein sporulation was inhibited by the serine protease inhibitors TLCK and TPCK. The association between proteolysis and differentiation apparent in this organism correlates with evidence of a similar phenomenon observed in the streptomycetes, suggesting that this may be a common response associated with differentiation in filamentous actinomycetes.
authors.affiliation: School of Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK. p.hoskisson@abdn.ac.uk
authors.forename: Paul A
authors.initials: PA
authors.lastname: Hoskisson
authors.forename: George P
authors.initials: GP
authors.lastname: Sharples
authors.forename: Glyn
authors.initials: G
authors.lastname: Hobbs
mesh_terms: D007700:Kinetics; D008847:Micromonospora / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000502:physiology; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000494:pharmacology
chemical_list: D015842:Serine Proteinase Inhibitors; D014107:Tosyllysine Chloromethyl Ketone; D014108:Tosylphenylalanyl Chloromethyl Ketone; D010447:Peptide Hydrolases