[Investigation of TiO2 photocatalytic degradation of bovine serum albumin].
The present paper reports photocatalytic degradation of bovine serum albumin (BSA) by TiO2 under near UV (365 nm) irradiation. Ultraviolet-Visible spectrometry, FTIR spectrometry and HPLC were used to study the degradation and characterize the final degradation products. It was found that under near UV irradiation, BSA (0.1 g x L(-1)) can be totally degraded to small fragments by TiQ2 in 5 h. Total mineralization of BSA (93.5%) is reached after reacting for 40 h. C, N and S in BSA are mineralized to give CO2, NO3- and SO4(2-). It is suggested that the degradation of BSA happens in two steps: first to small fragments, then further mineralized to small inorganic molecule. The absence of UV irradiation or TiQ2 can only lead to partial degradation of BSA (30%) in 5 h and no CO2 is observed even in longer reaction time.
Yang
Gui-qin
GQ
Research Institute of Photocatalysis, Fuzhou University, Fuzhou 350002, China.
Li
Zhao-hui
ZH
Lin
Zhang-xiang
ZX
Wang
Xu-xu
XX
Liu
Ping
P
Fu
Xian-zhi
XZ
D000818:Animals; D002245:Carbon Dioxide / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis / Q000528:radiation effects; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D009566:Nitrates / Q000737:chemistry / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*; D014466:Ultraviolet Rays
D009566:Nitrates; D013431:Sulfates; D002245:Carbon Dioxide; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D014025:Titanium