Elicitation of neutralizing antibodies by intranasal administration of recombinant vesicular stomatitis virus expressing human immunodeficiency virus type 1 gp120.
Recombinant viral vectors are useful tools for AIDS vaccine development. However, expression of HIV-1 envelope genes using viral vectors has not been successful in the induction of potent neutralizing antibodies in vivo. We took advantage of the strong immunogenicity of vesicular stomatitis virus (VSV)-based vector and expressed HIV-1 HXB2 gp120 gene in the recombinant VSV. Our results showed that HIV-1 gp120 protein expressed by the recombinant VSV retained the native conformation of the protein to some degree and was recognized by two well-characterized broad anti-HIV-1 neutralizing monoclonal antibodies b12, 2G12. We further showed that only one time intranasal immunization with the recombinant VSV led to production of anti-HIV-1 anti-sera in mice. In addition, we found that the anti-sera had the ability to neutralize not only HXB2 envelope-pseudotyped HIV-1 viruses but also HIV-1 pseudotyped viruses with JRFL envelopes. These results suggest that HIV-1 gp120 expressed by the recombinant VSV, in combination with the route of intranasal administration, is an effective strategy to evaluate the immunogenicity of HIV-1 envelope protein and its variants in mice.
Jiang
Pengfei
P
Department of Cell Biology, The College of Life Sciences, Peking University, Beijing 100871, China.
Liu
Yanxia
Y
Yin
Xiaolei
X
Yuan
Fei
F
Nie
YuChun
Y
Luo
Min
M
Aihua
Zheng
Z
Liyin
Du
D
Ding
Mingxiao
M
Deng
Hongkui
H
D000281:Administration, Intranasal; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005260:Female; D015870:Gene Expression; D015483:HIV Antibodies / Q000008:administration & dosage / Q000097:blood / Q000276:immunology* / Q000494:pharmacology*; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D051379:Mice; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000378:metabolism*
D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D011994:Recombinant Proteins