title: Concentration-dependent suppressive effect of shrimp head protein hydrolysate on dehydration-induced denaturation of lizardfish myofibrils.
abstract: To utilize fishery waste products as functional food material, the shrimp head protein hydrolysate (SHPH) was produced from three species of shrimp wastes, Northern pink shrimp, Endeavour shrimp and black tiger shrimp, by enzymatic hydrolysis. The SHPH was used as a natural food preservative by adding to lizardfish myofibrils at concentrations ranging from 2.5% to 10%. Their effects on the state of water and the denaturation of myofibrils during dehydration were evaluated. The amount of monolayer and multilayer water in myofibrils containing SHPH were higher than those without SHPH (control). DSC analyses revealed that the amount of unfrozen water increased significantly after addition of SHPH. The Ca-ATPase inactivation rate of myofibrils containing SHPH decreased during dehydration while 5-7.5% concentrations of SHPH exhibited optimum effect regardless of the species. The results implicated that SHPH can be used as an alternative food preservative for suppressive the dehydration-induced denaturation of myofibrils.
authors.affiliation: Graduate School of Science and Technology, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8521, Japan.
authors.forename: Yaowalux
authors.initials: Y
authors.lastname: Ruttanapornvareesakul
authors.forename: Misako
authors.initials: M
authors.lastname: Ikeda
authors.forename: Kenji
authors.initials: K
authors.lastname: Hara
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Osatomi
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Osako
authors.forename: Orawan
authors.initials: O
authors.lastname: Kongpun
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Nozaki
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005399:Fishes / Q000378:metabolism*; D005519:Food Preservation / Q000379:methods*; D009210:Myofibrils / Q000378:metabolism*; D033561:Penaeidae / Q000737:chemistry*; D011489:Protein Denaturation / Q000187:drug effects*; D011492:Protein Hydrolysates / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D020539:Sequence Analysis, Protein; D014867:Water / Q000737:chemistry
chemical_list: D011492:Protein Hydrolysates; D014867:Water; D000252:Calcium-Transporting ATPases