title: Impact of APOA5/A4/C3 genetic polymorphisms on lipid variables and cardiovascular disease risk in French men.
abstract: OBJECTIVE
  The goal of the present study was to assess the impact of 4 single nucleotide polymorphisms (SNPs) of APOA5/A4/C3 gene cluster on lipid levels and coronary heart disease (CHD) risk in French men.
  
  
  METHODS
  A total of 442 men with CHD were recruited from the university hospital and compared to 475 men free of CHD from the population of the same geographical area. The APOA5 S19W, APOA5 -l2,238T>C, APOA4 T347S and APOC3 -482C>T SNPs were examined.
  
  
  RESULTS
  The APOA5 S19W polymorphism was associated with plasma triglyceride levels. In multivariate logistic regression analyses the odds ratio (OR [95% Cl]) of hypertriglyceridemia (3rd vs. 1st tertile of triglyceride distribution) was 3.60 [1.38-9.42] in control subjects bearing at least one APOA5 19W variant. Haplotype analyses revealed a significant association between the 2111 haplotype and high triglyceride levels (+1.94 +/- 0.63 vs. 0.74 +/- 0.36 mmol/l for the 1111 haplotype p < 0.002). There was, in contrast, no significant difference in SNP distribution between CHD patients and controls. The age-adjusted OR of CHD were 1.46 [0.96-2.23], 0.79 [0.60-1.05], 0.91 [0.69-1.21] and 0.91 [0.69-l.22] in carriers of the APOA5 19W, APOA5 -12,238C, APOA4 347S and APOC3 -482T variants, respectively. There was also no significant difference in APOA5/A4/C3 haplotype distribution in patients and controls.
  
  
  CONCLUSIONS
  The APOA5 19W variant is associated with increased plasma triglycerides. However, there is no evidence that APOA5 S19W, -12,238T > C, APOA4 T347S and APCC3 -482C > T SNPs are major risk factors of CHD in French men.
authors.affiliation: Service d'Epidémiologie et de Santé Publique-INSERM U-508 & Departement d'atherosclerose, lnstitut Pasteur de Lille, France. jean.dallongeville@pasteur-lille.fr
authors.forename: Jean
authors.initials: J
authors.lastname: Dallongeville
authors.forename: Dominique
authors.initials: D
authors.lastname: Cottel
authors.forename: Michèle
authors.initials: M
authors.lastname: Montaye
authors.forename: Valérie
authors.initials: V
authors.lastname: Codron
authors.forename: Philippe
authors.initials: P
authors.lastname: Amouyel
authors.forename: Nicole
authors.initials: N
authors.lastname: Helbecque
mesh_terms: D000328:Adult; D000072040:Apolipoprotein A-V; D001053:Apolipoproteins; D001054:Apolipoproteins A / Q000097:blood* / Q000235:genetics; D001056:Apolipoproteins C / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002784:Cholesterol / Q000097:blood* / Q000235:genetics; D008076:Cholesterol, HDL / Q000097:blood / Q000235:genetics; D003327:Coronary Disease / Q000453:epidemiology / Q000235:genetics*; D005602:France / Q000453:epidemiology; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D014280:Triglycerides / Q000097:blood* / Q000235:genetics
chemical_list: C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001053:Apolipoproteins; D001054:Apolipoproteins A; D001056:Apolipoproteins C; D008076:Cholesterol, HDL; D014280:Triglycerides; D002784:Cholesterol