Immunolocalization of cyclin D1 in the developing lens of c-maf -/- mice.
The maf gene encodes a transcription factor protein containing a typical basic/leucine zipper domain structure, a motif for protein dimerization and DNA binding. It has been demonstrated that maf family genes have important roles in embryonic development and cellular differentiation. In this study, localization of cyclin D1, one of the cell cycle-related molecules, was examined immunohistochemically in developing lens cells of c-maf knockout (-/-) mice. At embryonic day 14 in wild-type mice, lens cells consisted of round epithelial cells in a single layer and regularly arranged elongated lens cells, indicating primary lens fiber cells. Cyclin D1-positive nuclei were observed in the lens epithelial cells, whereas cyclin D1 was not detected in the primary lens fiber cells. In c-maf -/- mice, a variety of round epithelial cells were located in the anterior and posterior lens. Many cyclin D1-positive nuclei were observed in lens epithelial cells as well as posterior lens cells. These results are consistent with c-maf playing a role in the regulation of cyclin D1 in developing lens cells.
Kase
Satoru
S
Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, N15 W7, Kita-ku, Sapporo 060-8638, Japan. kaseron@med.hokudai.ac.jp
Yoshida
Kazuhiko
K
Sakai
Masaharu
M
Ohgami
Kazuhiro
K
Shiratori
Kenji
K
Kitaichi
Nobuyoshi
N
Suzuki
Yukari
Y
Harada
Takayuki
T
Ohno
Shigeaki
S
D000818:Animals; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D019938:Cyclin D1 / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000737:chemistry* / Q000166:cytology / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D051257:Proto-Oncogene Proteins c-maf / Q000235:genetics* / Q000502:physiology*
D051257:Proto-Oncogene Proteins c-maf; D019938:Cyclin D1