title: Quantifying protein-protein interactions in the ubiquitin pathway by surface plasmon resonance.
abstract: The commercial availability of instruments, such as Biacore, that are capable of monitoring surface plasmon resonance (SPR) has greatly simplified the quantification of protein-protein interactions. Already, this technique has been used for some studies of the ubiquitin-proteasome system. Here we discuss some of the problems and pitfalls that researchers should be aware of when using SPR analyses for studies of the ubiquitin-proteasome system.
authors.affiliation: Department of Biochemistry, Institute for Molecular Biology and Physiology, Copenhagen University, Copenhagen, Denmark.
authors.forename: Rasmus
authors.initials: R
authors.lastname: Hartmann-Petersen
authors.forename: Colin
authors.initials: C
authors.lastname: Gordon
mesh_terms: D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods*; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D011506:Proteins; D025801:Ubiquitin