Genotoxicity studies on benzimidazole retinoids.
Retinoids consist of a family of naturally occuring compounds including all-trans retinoic acid (ATRA), retinal, retinol (vitamin A), 9-cis retinoic acid, 13-cis retinoic acid as well as a large number of synthetic derivatives. Retinoids are known to elicit diverse pharmacological profiles such as controlling cell differentiation/proliferation and modulating specific premalignant lesions and reducing second primary tumors in patients. Clinical use of retinoids is limited due to their toxicity. Three benzimidazole retinoid derivatives (BITN, BITNm, BITNe) were synthesized and were examined in terms of genotoxicity towards human lymphocyte cultures by sister chromatid exchange (SCE) analysis. It has been found that BITN decreased the number of SCEs 20% at 10(-6) M, but had no effect at 10(-5) M. No significant effect on SCEs was observed for BITNm and BITNe at both concentrations. ATRA increased the SCEs (35%) at 10(-5) M but had no effect at 10(-6) M. The results have shown that benzimidazole retinoids did not induce SCE significantly. Besides this, BITN reduced the SCEs and had a protective effect at low concentration. Since the induction of glutathione S-transferase (GST) is associated with anticancer drug resistance, the effects of BITN, BITNm, BITNe and ATRA on human lymphocyte GSTs were also investigated using CDNB as substrate. BITN and BITNm induced GST activities 54% and 49% respectively at 10(-5) M, but had no effect at 10(-6) M. BITNe induced GST activity 62% at 10(-5) M and 35% at 10(-6) M. ATRA had no effect on GST activity at 10(-5) M.
Balaban
F
F
Department of Biological Sciences, Middle East Technical University, Tandogan, Ankara, Turkey.
Ates-Alagoz
Z
Z
Buyukbingol
E
E
Iscan
M
M
D001562:Benzimidazoles / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D003979:Diazonium Compounds / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology; D009153:Mutagens; D012176:Retinoids / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects
C504881:4-carboxyl-2,6-dinitrobenzenediazonium ion; D001562:Benzimidazoles; D003979:Diazonium Compounds; D009153:Mutagens; D012176:Retinoids; D005982:Glutathione Transferase