title: Determination of different classes of amines with capillary zone electrophoresis and contactless conductivity detection.
abstract: The use of contactless conductivity detection for the determination of different organic amines in CE was successfully demonstrated. Aliphatic non UV-absorbing species could be determined along absorbing compounds by measuring the conductivity of their protonated forms. The species tested included short-chained aliphatic primary, secondary and tertiary amines, branched aliphatic amines, diamines, hydroxyl-substituted amines as well as species incorporating aromatic and non-aromatic cyclic moieties. Highest sensitivity was obtained with BGE solutions containing solely acetic acid. A concentration of 0.5 M at a pH value of 2.5 was used. Detection limits were in the order of 1 microM. Complete separation of cis- and trans-1,2-diaminocyclohexane could be achieved by adding 18-crown-6 as modifier to the electrolyte solution.
authors.affiliation: Department of Chemistry, University of Basel, Basel, Switzerland.
authors.forename: Xiao Yang
authors.initials: XY
authors.lastname: Gong
authors.forename: Peter C
authors.initials: PC
authors.lastname: Hauser
mesh_terms: D000085:Acetates / Q000737:chemistry; D000588:Amines / Q000032:analysis*; D002021:Buffers; D043844:Crown Ethers / Q000737:chemistry; D003514:Cyclohexylamines / Q000032:analysis / Q000737:chemistry; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D013237:Stereoisomerism
chemical_list: D000085:Acetates; D000588:Amines; D002021:Buffers; D043844:Crown Ethers; D003514:Cyclohexylamines; C015762:18-crown-6; C030435:1,2-cyclohexanediamine