Bone healing in surgically created defects treated with either bioactive glass particles, a calcium sulfate barrier, or a combination of both materials. A histological and histometric study in rat tibias.
OBJECTIVE
The purpose of this study was to histologically analyze the influence of bioactive glass and/or a calcium sulfate barrier on bone healing in surgically created defects in rat tibias.


METHODS
Sixty-four rats were divided into 4 groups: C (control), CS (calcium sulfate), BG (bioactive glass), and BG/CS (bioactive glass/calcium sulfate). A surgical defect was created in the tibia of each animal. In Group CS, a calcium sulfate barrier was placed to cover the defect. In Group BG the defect was filled with bioactive glass. In Group BG/CS, it was filled with bioactive glass and protected by a barrier of calcium sulfate. Animals were sacrificed at 10 or 30 days post-operative. The formation of new bone in the cortical area of the defect was evaluated histomorphometrically.


RESULTS
At 10 days post-operative, Group C presented significantly more bone formation than Groups CS, BG, or BG/CS. No statistically significant differences were found between the experimental groups. At 30 days post-operative, Group C demonstrated significantly more bone formation than the experimental groups. Groups CS and BG/CS showed significantly more bone formation than Group BG. No statistically significant differences were found between Group CS and BG/CS.


CONCLUSIONS
(a) the control groups had significantly more bone formation than the experimental groups; (b) at 10 days post-operative, no significant differences were found between any of the experimental groups; and (c) at 30 days post-operative, the groups with a calcium sulfate barrier had significantly more bone formation than the group that used bioactive glass only.
Melo
Luiz G N
LG
Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, University of State of São Paulo Júlio de Mesquita Filho-UNESP, Araçatuba, Brazil.
Nagata
Maria J H
MJ
Bosco
Alvaro F
AF
Ribeiro
Luciana L G
LL
Leite
Cristiane M
CM
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D018786:Bone Substitutes / Q000494:pharmacology*; D002133:Calcium Sulfate; D002516:Ceramics / Q000494:pharmacology*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D008297:Male; D008567:Membranes, Artificial; D051381:Rats; D013977:Tibia / Q000601:surgery
C022107:Bioglass; D018786:Bone Substitutes; D008567:Membranes, Artificial; D002133:Calcium Sulfate