Characterization of multimetric variants of ubiquitin carboxyl-terminal hydrolase L1 in water by small-angle neutron scattering.
Here, we illustrated that the morphological structures of ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) variants and Parkinson's disease (PD) exhibit good pathological correlation by a small-angle neutron scattering (SANS). UCH-L1 is a neuro-specific multiple functional enzyme, deubiquitinating, ubiquityl ligase, and also involved in stabilization of mono-ubiquitin. To examine the relationship between multiple functions of UCH-L1 and the configuration of its variants [wild-type, I93M (linked to familial Parkinson's disease), and S18Y (linked to reduced risk of Parkinson's disease)], in this report, we proposed that these were all self-assembled dimers by an application of a rotating ellipsoidal model; the configurations of these dimers were quite different. The wild-type was a rotating ellipsoidal. The globular form of the monomeric component deformed by the I93M mutation. Conversely, the S18Y polymorphism promoted the globularity. Thus, the multiple functional balance is closely linked to the intermolecular interactions between the UCH-L1 monomer and the final dimeric configuration.
Naito
Sachio
S
High Energy Accelerator Research Organization, 1-1 Oho, Tsukuba-shi, Ibaraki 305-0801, Japan. sachio@post.kek.jp
Mochizuki
Hideki
H
Yasuda
Toru
T
Mizuno
Yoshikuni
Y
Furusaka
Michihiro
M
Ikeda
Susumu
S
Adachi
Tomohiro
T
Shimizu
Hirohiko M
HM
Suzuki
Junichi
J
Fujiwara
Satoru
S
Okada
Tomoko
T
Nishikawa
Kaori
K
Aoki
Shunsuke
S
Wada
Keiji
K
D002942:Circular Dichroism; D006801:Humans; D008958:Models, Molecular; D033363:Neutron Diffraction; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014867:Water / Q000737:chemistry*
D011994:Recombinant Proteins; C507533:Ubiquitin carboxyl-Terminal Hydrolase L-1, human; D014867:Water; D043222:Ubiquitin Thiolesterase