[Effect of arnebia root oil in promoting the tissue recovery of surface of wound and basic fibroblast growth factor (bFGF) mRNA expression].
OBJECTIVE
To explore the molecular biological mechanism of Arnebia Root oil (AO) in promoting the recovery of surface of wound by observing basic fibroblast growth factor (bFGF) mRNA expression in the wound tissue and healing rate of the wound.


METHODS
Patients in the observed group with their wound treated by AO and those in the control group treated by petrolatum gauze. The wound surface healing rate was estimated and bFGF mRNA expression was observed by RT-PCR.


RESULTS
Endogenous bFGF mRNA expression existed in the wound surface of both groups, but its level in the observed group at any time point was obviously higher than that in the control group respectively, with significant difference in comparison of the gray density between the two groups (P < 0.05). The wound surface healing rate kept abreast with bFGF mRNA expression in wound tissues, so it was significantly higher in the observed group than that in the control group (P < 0.05). GAPDH gene, which was taken as a parameter for internal reference, expressed with a certain amount unchanged in different periods of healing (P > 0.05 ).


CONCLUSIONS
AO shows obviously promotive action on bFGF, an important regulatory factor on wound healing, it might complete the recovery process by stimulating the increase of bFGF.
Pei
Xian-wu
XW
Department of Orthpedics, The Second Hospital Affiliated ro Xi' an Jiaotong University, Xi' an. peixianwu@126.com
Wang
Kun-zheng
KZ
Chen
Jun-chang
JC
Dang
Xiaoqian
X
Shi
Zhibin
Z
Gao
Dengfeng
D
D000293:Adolescent; D000328:Adult; D028082:Boraginaceae / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D008517:Phytotherapy; D010938:Plant Oils / Q000008:administration & dosage / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D010938:Plant Oils; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2