EcoRI polymorphism of the metastasis-suppressor gene NME1 in Mexican patients with breast cancer.
Human breast cancer cells with high metastatic potential show reduced expression of the metastasis-suppressor gene NME1. There are two polymorphic sites for the restriction enzymes BglII and EcoRI, both detectable by Southern blot analysis. Although the BglII site has been analyzed for loss of heterozygosity, the biallelic EcoRI site polymorphism has not been studied in association with breast cancer, complications or metastasis. We analyzed EcoRI site allele frequencies in Mexican patients with breast cancer, using polymerase chain reaction -restriction fragment length polymorphisms. The polymorphic allelic frequencies in the cases and reference groups were 0.4215 and 0.3375, respectively; this difference was not statistically significant (chi2=0.8687, p=0.3512). Thus, EcoR1 polymorphic site was not associated with breast cancer in this series, but could be analyzed in association with metastases and might be informative in the evaluation of loss of heterozygosity in women with breast cancer.
Rubio
Susan Andrea Gutierrez
SA
Medicina Molecular Division, Centro de Investigacion Biomedica de Occidente, Guadalajara, Jalisco, Mexico. suanguru@yahoo.com.mx
Martinez
Silvia Esperanza Flores
SE
Corona
Jose Sanchez
JS
Ruiz
Adriana Patricia Mendizabal
AP
Rincon
Angel Emilio Suarez
AE
Lagunas
Ignacio Arevalo
IA
Camacho
Jose Gonzalo Vazquez
JG
Moguel
Maria Cristina Moran
MC
D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D008800:Mexico; D009362:Neoplasm Metastasis; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000032:analysis; D044465:White People / Q000235:genetics
D012333:RNA, Messenger