title: Hydrolysis of plasmid DNA and RNA by amino alkyl naphthalimide as metal-free artificial nuclease.
abstract: A strategy of dimethylamino alkyldiimide conjugated with an intercalator of naphthalimide for hydrolysis of DNA was suggested and evaluated. 4 can hydrolyze 4 kb plasmid DNA into 2 kb fragments with GC and GG selectivity, which represents a novel example of sequence- or site-selective metal-free DNA artificial nuclease. Results also show it could hydrolyze RNA efficiently.
authors.affiliation: Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116024, China. qingyang@dlut.edu.cn
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Xu
authors.forename: Yuanshe
authors.initials: Y
authors.lastname: Sun
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Li
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Li
authors.forename: Xuhong
authors.initials: X
authors.lastname: Qian
mesh_terms: D004247:DNA / Q000737:chemistry*; D003851:Deoxyribonucleases / Q000138:chemical synthesis / Q000737:chemistry*; D015195:Drug Design; D006860:Hydrogen Bonding; D006868:Hydrolysis; D007094:Imides / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D009281:Naphthalenes / Q000138:chemical synthesis / Q000737:chemistry*; D010957:Plasmids; D012313:RNA / Q000737:chemistry*; D012260:Ribonucleases / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D007094:Imides; D009281:Naphthalenes; D012313:RNA; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases