Interleukin-1 receptor antagonist gene polymorphism and traditional classification in obese women.
Cytokines appear to be the major regulators of adipose tissue metabolism. Interleukin- 1 receptor antagonist (IL-1ra) serum levels are increased in human obesity, and are under strong genetic control. The hypothesis was tested that the IL-1ra gene might be a candidate for obesity. Furthermore, the relationship was investigated between Sasang constitution and IL-1ra polymorphism. The frequency of a penta-allelic 86-bp tandem repeat (VNTR) in the intron 2 of IL-1ra gene in 67 lean (BMI<25 kg/m2), 133 overweight (BMI 25-29.9 kg/m2) and 61 obese (BMI>or=30 kg/m2) otherwise healthy Korean subjects was investigated. Total fat mass and percentage body fat were determined by dual-energy X-ray absorptiometry. Subjects were discriminated into four types by QSCC II program as well as clinical data (weight, height, blood pressure, etc.); Teaeumin, Taeyangin, Soyangin, and Soeumin. Genomic DNA was extracted and used for polymerase chain reaction-based genotyping of IL-1ra. The genotypic, or allelic distribution did not differ markedly between the three groups. The relative risk of being obese in comparison with lean was twofold increased in allele 2 carriers, although it was not statistically significant. Carriers of the allele 2 did not show a significant difference in physical and clinical characteristics. However, the relative risk of being obese in comparison with lean was increased in Taeumin subjects (p=.050), and so was in IL-1ra A2- carriers (p=.047). No relationship was found between the IL-1ra polymorphism and BMI in Korean women, but the authors first attempted to find an association among IL-1ra polymorphism, obesity, and Sasang constitution.
Um
Jae-Young
JY
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, Seoul, South Korea.
Kim
Hyung-Min
HM
Mun
Sung-Won
SW
Song
Yung-Sun
YS
Hong
Seung-Heon
SH
D000293:Adolescent; D000328:Adult; D001823:Body Composition / Q000235:genetics*; D015992:Body Mass Index; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D008875:Middle Aged; D009765:Obesity / Q000145:classification / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors; D012016:Reference Values; D012795:Sialoglycoproteins / Q000235:genetics*; D013008:Somatotypes / Q000235:genetics*
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins