Discrimination of four soybean dwarf virus strains by dot-blot hybridization with specific probes.
Soybean dwarf virus (SbDV) is divided into four strains (YS, YP, DS, and DP) on the basis of host symptoms in infected soybean plants and on aphid vector specificity. To detect and discriminate each strain of SbDV by dot-blot hybridization, probes Y, D, S, and P were prepared. Probes Y and D, covering most of the 3'-noncoding region of the viral genome containing the sequence of small subgenomic RNA, could discriminate strains in accord with the host symptoms. Probes S and P were derived from the 5'-half of open reading frame 5 encoding the N-terminal half of the readthrough domain which is closely related to the aphid vector specificity of each strain. Thus, the four SbDV strains could be discriminated by the combination of these probes. This method, based on a procedure specific to the SbDV sequence, is a good alternative for routine examination of infected plants in soybean breeding programs for evaluation of resistance to SbDV and for assessment of the distribution of each strain in epidemiological studies.
Yamagishi
Noriko
N
National Agricultural Research Center for Tohoku Region, Shimo-kuriyagawa, Morioka, Iwate, Japan.
Terauchi
Hidetaka
H
Honda
Ken-ichiro
K
Kanematsu
Seiji
S
Hidaka
Soh
S
D017793:Luteovirus / Q000145:classification / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015347:RNA Probes; D012367:RNA, Viral / Q000235:genetics*; D013025:Glycine max / Q000821:virology*
D015347:RNA Probes; D012367:RNA, Viral