Fast high performance liquid chromatography and ultraviolet-visible quantification of principal phenolic antioxidants in fresh rosemary.
An improved HPLC method is reported for the determination of rosemary's principal phenolic antioxidants, rosmarinic and carnosic acids, providing a fast and simultaneous determination for both of them by using a solid phase column. The analysis was performed with fresh methanolic extractions of Rosmarinus officinalis. To quantify the amount of antioxidants in a fast and reproducible way by means of UV-vis absorption measurements, a spectrophotometric multi-wavelength calibration curve was constructed based on the antioxidant contents obtained with the recently developed HPLC method. This UV-vis methodology can be extended to the determination of other compounds and herbs if the restrictions mentioned in the text are respected.
Troncoso
N
N
Fundación Chile, Area Agroindustria, Parque Rabat 6165 Vitacura, Santiago, Chile.
Sierra
H
H
Carvajal
L
L
Delpiano
P
P
Günther
G
G
D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D010945:Plants, Edible / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D000975:Antioxidants; D010636:Phenols