title: Use of trypan blue for penetrating keratoplasty.
abstract: Use of trypan blue for penetrating keratoplasty was developed to facilitate the procedure. Trypan blue is injected before and after the addition of 0.25 mL of an ophthalmic viscosurgical device (OVD), sodium hyaluronate, to stain the internal and external cut edge of the cornea as well as the OVD, enabling the surgeon to improve visualization of the incision and suture depth, improve alignment of host and donor tissues, and ensure that all OVD is removed.
authors.affiliation: Department of Medicine, Addenbrooke's Hospital, University of Cambridge, Cambridge, United Kingdom. jcpr2@cam.ac.uk
authors.forename: Jonathan C P
authors.initials: JC
authors.lastname: Roos
authors.forename: Malcolm G
authors.initials: MG
authors.lastname: Kerr Muir
mesh_terms: D004396:Coloring Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D006820:Hyaluronic Acid / Q000627:therapeutic use; D007267:Injections; D015948:Keratoplasty, Penetrating / Q000379:methods*; D013194:Staining and Labeling; D013536:Suture Techniques; D014343:Trypan Blue / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D004396:Coloring Agents; D006820:Hyaluronic Acid; D014343:Trypan Blue