Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro.
Polysaccharide extracted from Ulva pertusa (Chlorophyta) is a group of sulfated heteropolysaccharide; for simplicity, the sulfated polysaccharide is referred to as ulvan in this paper. In this study, different sulfate content ulvans were prepared with sulfur trioxide/N,N-dimethylformamide (SO3-DMF) in formamide, and their antioxidant activities were investigated including scavenging activity of superoxide and hydroxyl radicals, reducing power and metal chelating ability. As expected, we obtained several satisfying results, as follows: firstly, high sulfate content ulvans had more effective scavenging activity on hydroxyl radical than natural ulvan. Secondly, comparing with natural ulvan, high sulfate content ulvans exhibited stronger reducing power. Thirdly, HU4 (sulfate content, 30.8%) and HU5 (sulfate content, 32.8%) showed more pronounce chelating ability on ferrous ion at high concentration than other samples.
Qi
Huimin
H
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China. qihuimin@ms.qdio.ac.cn
Zhang
Quanbin
Q
Zhao
Tingting
T
Chen
Rong
R
Zhang
Hong
H
Niu
Xizhen
X
Li
Zhien
Z
D000975:Antioxidants / Q000737:chemistry*; D004126:Dimethylformamide; D005559:Formamides / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D013431:Sulfates / Q000737:chemistry*; D013461:Sulfur Oxides / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry; D044445:Ulva / Q000378:metabolism*
D000975:Antioxidants; D005559:Formamides; D016166:Free Radical Scavengers; D011134:Polysaccharides; D013431:Sulfates; D013461:Sulfur Oxides; D013481:Superoxides; D017665:Hydroxyl Radical; C031066:formamide; D004126:Dimethylformamide; C011118:sulfur trioxide