title: [Mechanism of AcrAB-TolC system mediated multiple antimicrobial resistance in Salmonella typhimurium].
abstract: OBJECTIVE
  To confirm AcrAB-TolC system mediated the multiple antimicrobial resistance in Salmonella typhimurium.
  
  
  METHODS
  One-step knock-out of chromosomal method was used, to disrupt chromosomal genes, in Salmonella typhimurium wild strain X2. The acrAB gene and tolC gene were knocked out and two mutants of the delta acrABX2 and delta tolCX2 were produced.
  
  
  RESULTS
  Results of E-test to two mutation strains and their parent strain showed that delta acrABX2 and delta tolCX2 were resistant to vancomycin and susceptible to amikacin and cephalothin, which was similar to their parents isolate X2, but (acrABX2 and (tolCX2 were susceptible to other eleven antimicrobial agents, while the parents isolate X2 was resistant to those.
  
  
  CONCLUSIONS
  multiple antimicrobial resistance in Salmonella typhimurium. It suggested that the AcrAB-TolC system mediated the multiple antimicrobial resistance in Salmonella typhimurium.
authors.affiliation: National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Yun-Chang
authors.initials: YC
authors.lastname: Guo
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Du
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Liu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D055786:Gene Knockout Techniques; D009154:Mutation; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics*; D020713:Vancomycin Resistance
chemical_list: C493251:AcrAB-TolC protein, Salmonella enterica; D001426:Bacterial Proteins; D002352:Carrier Proteins