title: [Blockage of allergic rhinitis in mice with recombinant protein of cytotoxic T lymphocyte associated antigen-4 extracellular domain].
abstract: OBJECTIVE
  To explore a new immunotherapy against allergic rhinitis.
  
  
  METHODS
  The recombinant protein of CTLA4 extracellular domain was obtained through construction of CTLA4-yeast expression system. The allergic rhinitis in mice was induced by sensitizing and challenging with ovalbumin (OVA). The allergic rhinitis related symptoms and the morphological changes in nasal mucosa were compared between the allergic rhinitis group and the CTLA4 extracellular domain group treated with CTLA4 extracellular domain before each challenge by ways of intraperitoneal injection.
  
  
  RESULTS
  CTLA4 extracellular domain with a molecular weight of 28 000, which was confirmed by Western blot, could be generated through CTLA4-yeast expression system. The purified CTLA4 extracellular domain could inhibit T cells proliferation in mixed lymphocyte reaction with a inhibitory rate of 95.4%. The mice in allergic rhinitis group appeared typical allergic rhinitis symptoms after OVA challenge, such as rhinorrhea and sneeze. Meanwhile the nasal pathological studies showed edema and congestion in mucosa tissue and local influx of inflammatory cells. Whereas in CTLA4 extracellular domain group, the nasal symptoms were rarely observed, and the pathological change in nasal mucosa was significantly abated.
  
  
  CONCLUSIONS
  The protein of CTLA4 extracellular domain could prevent the allergic rhinitis in mice. The underlying mechanism of which might be the inhibition of the T cell activation.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tong Ren Hospital Affiliated to Capital University of Medical Sciences, Beijing 100730, China. zhujok@yahoo.com.cn
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
authors.forename: De-Min
authors.initials: DM
authors.lastname: Han
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: He
authors.forename: Xi-Wei
authors.initials: XW
authors.lastname: Chen
authors.forename: Shao-Xuan
authors.initials: SX
authors.lastname: Yi
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D060908:CTLA-4 Antigen; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000473:pathology*; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*
chemical_list: D015703:Antigens, CD; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; C556707:Ctla4 protein, mouse; D011994:Recombinant Proteins; D010047:Ovalbumin