Glycated albumin and cross-linking of CD44 induce scavenger receptor expression and uptake of oxidized LDL in human monocytes.
Functional role of CD44, a principal receptor of hyaluronan, and glycated albumin for differentiation of resting human monocytes isolated by counterflow centrifugal elutriation was investigated. Flow cytometric analysis revealed that amadori-modified glycated albumin induced expression of CD44 as well as macrophage scavenger receptors (MSRs) such as CD36 and CD68 on resting monocytes. Crosslinking of CD44 on monocytes also induced MSR expression. Furthermore, CD44 crosslinking and/or glycated albumin enhanced the uptake of oxidized-low density lipoprotein in monocytes and foam cell formation. Taken together, engagement of CD44 (e.g., hyaluronan) and glycated albumin induced the differentiation of resting monocytes into foam macrophages through the induction of MSRs, implying that CD44 could be involved in atherosclerotic lesions of those such as diabetic patients.
Kishikawa
Hirofumi
H
First Department of Internal Medicine, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Mine
Shinichiro
S
Kawahara
Chie
C
Tabata
Takahiro
T
Hirose
Akiko
A
Okada
Yosuke
Y
Tanaka
Yoshiya
Y
D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D017127:Glycation End Products, Advanced; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000493:pharmacokinetics*; D009000:Monocytes / Q000378:metabolism*; D051116:Receptors, Scavenger / Q000378:metabolism*; D012709:Serum Albumin / Q000008:administration & dosage*; D000093423:Glycated Serum Albumin
D003432:Cross-Linking Reagents; D017127:Glycation End Products, Advanced; D018960:Hyaluronan Receptors; D008077:Lipoproteins, LDL; D051116:Receptors, Scavenger; D012709:Serum Albumin; C099252:oxidized low density lipoprotein; D000093423:Glycated Serum Albumin