Hyperbaric oxygen reduces delayed immune-mediated neuropathology in experimental carbon monoxide toxicity.
The goal of this investigation was to determine whether exposure to hyperbaric oxygen (HBO(2)) would ameliorate biochemical and functional brain abnormalities in an animal model of carbon monoxide (CO) poisoning. In this model, CO-mediated oxidative stress causes chemical alterations in myelin basic protein (MBP), which initiates an adaptive immunological response that leads to a functional deficit. CO-exposed rats do not show improvements in task performance in a radial maze. We found that HBO(2) given after CO poisoning will prevent this deficit, but not eliminate all of the CO-mediated biochemical alterations in MBP. MBP from HBO(2) treated CO-exposed rats is recognized normally by a battery of antibodies, but exhibits an abnormal charge pattern. Lymphocytes from HBO(2)-treated and control rats do not become activated when incubated with MBP, immunohistological evidence of microglial activation is not apparent, and functional deficits did not occur, unlike untreated CO-exposed rats. The results indicate that HBO(2) prevents immune-mediated delayed neurological dysfunction following CO poisoning.
Thom
Stephen R
SR
Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, 19104-6068, USA. sthom@mail.med.upenn.edu
Bhopale
Veena M
VM
Fisher
Donald
D
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D002249:Carbon Monoxide Poisoning / Q000150:complications / Q000276:immunology* / Q000628:therapy; D002493:Central Nervous System Diseases / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D004676:Myelin Basic Protein / Q000187:drug effects / Q000276:immunology; D009474:Neurons / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010100:Oxygen / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
D004676:Myelin Basic Protein; D010100:Oxygen