Optimization of recombinant hyperthermophilic esterase production from agricultural waste using response surface methodology.
The aim of this work was to evaluate the capability of corn steep liquor being a low cost substrate on the recombinant protein production by cultivating recombinant Escherichia coli and increasing the production of hyperthermophilic esterase (HE). The effect of corn steep liquor, mineral salt and trace metals on hyperthermophilic esterase production was investigated by means of a five-level three-factor central composite rotatable design. Optimized values of the factors were determined and a maximum hyperthermophilic esterase production of 251.39 U/ml was obtained. This value equaled the yield by yeast extract and peptone medium on the whole.
Ren
Xiaodong
X
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, PR China.
Yu
Dawei
D
Han
Siping
S
Feng
Yan
Y
D041522:Aeropyrum / Q000235:genetics; D019149:Bioreactors; D018556:Crops, Agricultural; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D004950:Esterases / Q000096:biosynthesis*; D008962:Models, Theoretical; D030781:Organisms, Genetically Modified / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis*; D003313:Zea mays
D011994:Recombinant Proteins; D004950:Esterases