Expression and immunohistochemical localization of Cdc2 and P70S6K in different stages of mouse germ cells.
In order to determine the function and possible relationship between Cdc2 and P(70)S6K, Western blot analysis and immunohistochemistry analysis were used to study the expression and kinase activity of Cdc2 and P(70)S6K in male mouse germ cells. With the maturation of germ cells in the testis, the expression of Cdc2 and P(70)S6K was relatively constant. However, the kinase activity of P(70)S6K was increased and the phosphorylation of Tyr15 residue of Cdc2 was enhanced, which suggests that the kinase activity of Cdc2 is decreasing. Immunohistochemistry analysis also showed that there was a P(70)S6K transfer from nucleus to cytoplasm during spermatogenesis. During spermatogenesis, cell division of the germ cell in male mouse is decelerated; nevertheless, cell growth is enhanced. Cdc2 and P(70)S6K are involved in these two processes. It could be an alternative mechanism to prepare for future fertilization that Cdc2 is able to maintain a subtle balance between the production and growth of male germ cells by regulating P(70)S6K.
Yu
Bing-Zhi
BZ
Department of Biochemistry, China Medical University, Shenyang, People's Republic of China. ybzbiochem@yeah.net
Song
Yu-Tong
YT
Yu
Da-Hai
DH
Su
Wen-Hui
WH
Gasana
Venuste
V
Li
Yan-Xiao
YX
Zhang
Zhe
Z
D000818:Animals; D015153:Blotting, Western; D016203:CDC2 Protein Kinase / Q000032:analysis / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D008297:Male; D016200:Maturation-Promoting Factor / Q000502:physiology; D051379:Mice; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000032:analysis / Q000096:biosynthesis*; D013090:Spermatocytes / Q000201:enzymology; D013094:Spermatozoa / Q000201:enzymology*
D038762:Ribosomal Protein S6 Kinases, 70-kDa; D016203:CDC2 Protein Kinase; D016200:Maturation-Promoting Factor