[Studies on immunoregulation of polysaccharides-la from Radix Cynanchi Bungei].
OBJECTIVE
To study the effects of PRCB1a (one component of polysaccharides from Radix Cynanchi Bungei) on transformation of T lymphocytes of rabbit in vitro and immune function in mice.


METHODS
Three doses of PRCB1a (2,4,6 g x L(-1)) were respectively put in bottle with PHA and blood of rabbit. The effect of PRCB1a on immunity in vitro was studied by observing transformation of T lymphocytes; The dosage of PRCB1a (50,100,150 mg x kg(-1) x d(-1)) was given orally for seven days. The effects on immune function were investigated in mice.


RESULTS
Three doses of PRCB1a could significantly promote (P < 0.01) the ability of T lymphocytes proliferation; PRCB1a could improve the mouse thymus and spleen index, the celiac macrophage ability of engulfing CRBC, the delayed type hypersensitivity ability and the macrophage engulfing carbon granula ability.


CONCLUSIONS
The results indicate PRCB1a can enhance nonspectific and specific cellular immune function.
Gao
Li-jun
LJ
College of Pharmacy, Taishan Medical University, Taian 271000, China. ljgao.tsmc@126.com
Wang
Jian-hua
JH
Cui
Jian-hua
JH
Wang
Han-zhong
HZ
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D031105:Cynanchum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011817:Rabbits; D011897:Random Allocation; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D000276:Adjuvants, Immunologic; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides