Cost-effective application of pulsed-field gel electrophoresis to typing of Salmonella enterica Serovar Typhimurium.
Salmonella enterica serovar Typhimurium is frequently isolated from humans and animals. Phage typing is historically the first-line reference typing technique in Europe. It is rapid and convenient for laboratories with appropriate training and experience, and costs of consumables are low. Phage typing and pulsed-field gel electrophoresis (PFGE) were performed on 503 isolates of serovar Typhimurium. Twenty-nine phage types and 53 PFGE patterns were observed. Most isolates of phage types DT104, DT104b, and U310 are not distinguishable from other members of their phage type by PFGE. By contrast, PFGE of isolates of phage types DT193 and U302 shows great heterogeneity. Analysis of experience with PFGE and phage typing can facilitate the selective application of PFGE to maximize the yield of epidemiologically relevant additional information while controlling costs.
Doran
Geraldine
G
National Salmonella Reference Laboratory, University College Hospital, Galway, Ireland. Geraldine.Doran@nuigalway.ie
Morris
Dearbhaile
D
O'Hare
Colette
C
DeLappe
Niall
N
Bradshaw
Bernard
B
Corbett-Feeney
Geraldine
G
Cormican
Martin
M
D000818:Animals; D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D012483:Salmonella Phages / Q000145:classification* / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000145:classification* / Q000302:isolation & purification / Q000821:virology