title: An alpha/beta-fold C--C bond hydrolase is involved in a central step of nicotine catabolism by Arthrobacter nicotinovorans.
abstract: The enzyme catalyzing the hydrolytic cleavage of 2,6-dihydroxypseudooxynicotine to 2,6-dihydroxypyridine and gamma-N-methylaminobutyrate was found to be encoded on pAO1 of Arthrobacter nicotinovorans. The new enzyme answers an old question about nicotine catabolism and may be the first C--C bond hydrolase that is involved in the biodegradation of a heterocyclic compound.
authors.affiliation: Institut für Biochemie und Molekularbiologie, Universität Freiburg, Hermann-Herder-Str. 7, 79104 Freiburg, Germany.
authors.forename: Paula
authors.initials: P
authors.lastname: Sachelaru
authors.forename: Emile
authors.initials: E
authors.lastname: Schiltz
authors.forename: Gabor L
authors.initials: GL
authors.lastname: Igloi
authors.forename: Roderich
authors.initials: R
authors.lastname: Brandsch
mesh_terms: D000470:Alkaloids / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D023061:Gene Order; D006571:Heterocyclic Compounds / Q000378:metabolism; D006867:Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D009538:Nicotine / Q000031:analogs & derivatives / Q000378:metabolism*; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics; D011725:Pyridines / Q000378:metabolism
chemical_list: D000470:Alkaloids; D006571:Heterocyclic Compounds; D011725:Pyridines; C014173:pseudonicotine; C089431:2,6-dihydroxypyridine; D009538:Nicotine; D006867:Hydrolases