[The role of catalases in protection of proteins against oxidation in Saccharomyces cerevisiae utilizing ethanol as a carbon source].
The content of protein carbonyls and thiobarbituric acid reactive substances (TBARS) in the wild and catalase-deficient strains of the yeast Saccharomyces cerevisiae grown in glucose and ethanol media are compared. The deficient strain cells reproduced 10.6-fold slower in ethanol-containing medium. Activity of glucose-6-phosphate dehydrogenase in YWT1 cells was 1.7-fold lower when yeast are grown in ethanol, and content of protein carbonyls was 4.7-fold higher, than when they are grown in the medium with glucose. At the same time, reproduction of the wild type cells in ethanol was 2.7-fold slower and carbonyl groups of protein content was 2-fold lower, than under cultivation in glucose. TBARS content in both strains was similar when they were grown in ethanol and in glucose. It has been supposed that catalases play a certain role in the protection of S. cerevisiae proteins against oxidative modification when they are grown on the media with glucose and ethanol.
Hospodar'ov
D V
DV
Mandryk
S Ia
SIa
Lushchak
V I
VI
D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism; D000431:Ethanol / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D008054:Lipid Peroxides; D029701:Saccharomyces cerevisiae Proteins; D017392:Thiobarbituric Acid Reactive Substances; D000431:Ethanol; D002374:Catalase; D005947:Glucose