Tissue distribution of two field isolates and two vaccine strains of porcine parvovirus in foetal organs after experimental infection of pregnant sows as determined by real-time PCR.
The aim of this study was to investigate the tissue distribution of two different field isolates and two vaccine strains of porcine parvoviruses (PPV) in infected piglets after transplacental infection. The viral load in 10 different foetal organs was determined by real-time polymerase chain reaction assays with SYBR Green targeting the viral VP2 gene and the genomic c-myc gene in 12 foetuses. The viral load in foetal tissues differed greatly among the different parvoviruses. Between one virulent field isolate compared with the other field isolate and the vaccine strains, the detected viral copy number differed in an order of magnitude of 10(9). The virulent isolate contained PPV in all 10 organs with viral loads varying between 10(11) and 10(15) per 10(6) cells. Concerning the other field isolate and the two vaccine strains, if PPV was detected, in most of the cases the highest viral load was found in foetal kidneys with a maximum viral load of 10(3) per 10(6) cells. Additionally, PPV was found in the heart of one foetus, in the liver and duodenum of one foetus and in the thymus of one foetus with viral loads varying between 10(2.1) and 10(3.5) per 10(6) cells. In completely mummified foetuses with no discriminable organs of foetuses infected with the vaccine strains and the less virulent isolate, PPV was present in very low amounts or even below the detection limit.
Wilhelm
S
S
Institut für Tierhygiene und Offentliches Veterinärwesen, An den Tierkliniken 1, 04103 Leipzig, Germany.
Zeeuw
E J L
EJ
Selbitz
H-J
HJ
Truyen
U
U
D000825:Animal Structures / Q000196:embryology / Q000473:pathology / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005333:Fetus / Q000473:pathology / Q000821:virology*; D010322:Parvoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D028323:Parvovirus, Porcine / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000517:prevention & control / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014765:Viral Vaccines
D000914:Antibodies, Viral; D004279:DNA, Viral; D014765:Viral Vaccines