[The protective effect of recombinant Chinese interferon-gamma in pulmonary injury in mice].
OBJECTIVE
To observe the protective role of recombinant Chinese interferon-gamma (INF-gamma) in pulmonary injury (PI) induced by bleomycin (BLM) in C57 mice.


METHODS
Seventy-five C57 mice were randomly divided into a control group and groups treated with BLM, BLM + INF-gamma minimum dose (0.25 microg/d), BLM + INF-gamma medium dose (0.5 microg/d), and BLM + INF-gamma maximum dose (1.0 microg/d, with 15 mice each). PI was induced by BLM, and intervention with different doses of INF-gamma was carried out in the experiment groups, but no treatment was administered in the control mice. Measurement of pulmonary hydroxyproline (Hyp), image analysis of collagen I and III, and measurement of the ratio between lung alveoli and interstitial areas were performed.


RESULTS
Lung Hyp content and collagen I and III deposition were increased as compared with the control after administration of BLM (0.78 +/- 0.08 vs 0.65 +/- 0.06, P < 0.01; 0.048 +/- 0.006 vs 0.004 +/- 0.001, P < 0.01). Hyp content and collagen I and III deposition were reduced in group INF-gamma maximum dose (0.67 +/- 0.08 vs 0.78 +/- 0.08, P < 0.05; 0.008 +/- 0.001 vs 0.048 +/- 0.006, P < 0.01). The ratio of lung collagen to lung tissue areas was increased with the increase of INF-gamma doses (1.78, 0.12, 0.67, 0.73, 1.65 respectively).


CONCLUSIONS
INF-gamma is effective in alleviating BLM induced pulmonary injury in mice, possibly by inhibition of transformation of fibroblasts to myofibroblasts and collagen synthesis.
Huang
Shan-ying
SY
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Song
Liang-wen
LW
Liu
Tao
T
Wang
Qing-ming
QM
Li
Yang
Y
Zhang
Yong
Y
Sun
Li
L
D000818:Animals; D001761:Bleomycin / Q000009:adverse effects; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D004195:Disease Models, Animal; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism; D007371:Interferon-gamma / Q000627:therapeutic use*; D008168:Lung / Q000737:chemistry; D055370:Lung Injury / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins
D024042:Collagen Type I; D024061:Collagen Type III; D011994:Recombinant Proteins; D001761:Bleomycin; D007371:Interferon-gamma; D006909:Hydroxyproline