5'-Porphyrin-oligonucleotide conjugates: neutral porphyrin-DNA interactions.
[chemical reaction: see text]. Incorporation of hydrophilic tetraarylporphyrin phosphoramidites into the 5'-termimus of the DNA as well as noncharged porphyrin-DNA interactions have been studied. Porphyrin-modified oligonucleotides show lower melting temperatures than their unmodified analogues. Single-stranded DNA interacts more strongly with porphyrin and causes more intense chiral disturbance in the porphyrin environment than the corresponding double strand.
Balaz
Milan
M
Department of Chemistry, Columbia University, New York, New York 10027, USA. milan.balaz@chem.ox.ac.uk
Steinkruger
Jay D
JD
Ellestad
George A
GA
Berova
Nina
N
D001482:Base Composition; D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry* / Q000378:metabolism
D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D011166:Porphyrins; C434331:phosphoramidite; D004247:DNA