[Immunohistochemical localization of leucine-rich proteoglycans in the developing periodontal tissues of mice].
OBJECTIVE
To study the distribution and expression of fibromodulin, decorin and biglycan in developing normal periodontal tissues, so as to understand its role in periodontal tissue formation.


METHODS
Thirty six BALB/c mice in different developing stages were killed and their bilateral mandibular first molars with surrounding alveolar bones and gingival tissues were taken out, Power Vision two steps immunohistochemical method with anti-fibromodulin, anti-decorin and anti-biglycan was used to detect the tissue distribution and cellular localization of fibromodulin and related proteoglycans, decorin and biglycan.


RESULTS
Fibromodulin was strongly expressed in the subcutaneous gingival connective tissue, periodontal ligament, mainly in gingival and periodontal fibroblasts as well as their matrices. Strong expression was also noted in the area close to the interfaces of periodontal ligament-alveolar bone and periodontal ligament-cementum. Decorin was strongly expressed in the area of gingival connective tissue, periodontal ligament and the surface of alveolar bone, while biglycan was stained evidently in gingival connective tissue throughout the period of investigation, but negative in the surface of alveolar bone and osteoblasts.


CONCLUSIONS
Fibromodulin may interact with decorin and biglycan to regulate the network formation of gingival connective tissues and periodontal collagen fibers, and may be involved in mineralization of the alveolar bone and cementum.
Li
Shu
S
Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, China.
Yang
Pi-shan
PS
Pan
Ke-qing
KQ
Xie
Rui-yue
RY
Duan
Xue-jing
XJ
D000539:Alveolar Process / Q000166:cytology* / Q000254:growth & development; D000818:Animals; D058578:Biglycan; D058575:Decorin; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D000071235:Fibromodulin; D005881:Gingiva / Q000737:chemistry* / Q000254:growth & development; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000737:chemistry*; D010513:Periodontal Ligament / Q000737:chemistry* / Q000254:growth & development; D011509:Proteoglycans / Q000032:analysis*; D014083:Tooth Germ / Q000737:chemistry
C546885:Bgn protein, mouse; D058578:Biglycan; C546879:Dcn protein, mouse; D058575:Decorin; D016326:Extracellular Matrix Proteins; C000604332:Fmod protein, mouse; D011509:Proteoglycans; D000071235:Fibromodulin