Involvement of polyubiquitin chains via specific chain linkages in stress response in mammalian cells.
Polyubiquitination plays key roles in various proteasome-dependent and independent cellular events. To elucidate roles in stress response of polyubiquitin chains formed via specific chain linkages in mammalian cells, we established NIH3T3 stable cell lines that are capable of conditionally expressing K29R, K48R and K63R ubiquitin mutants, in which the Lys29, Lys48 and Lys63 residues of ubiquitin had been changed to Arg, and we examined the effects of various stresses on their cell viabilities. The expression of K63R ubiquitin mutant decreased viability of the cells post-exposed to ethanol, H(2)O(2) and methyl methanesulfonate (MMS), while that of K48R mutant decreased viability of the cells post-exposed to heat shock as well as ethanol, H(2)O(2) and MMS. Thus, these results suggest that polyubiquitin chains formed via specific chain linkages are involved in the respective stress responses in mammalian cells.
Fujimuro
Masahiro
M
Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Nishiya
Tadashi
T
Nomura
Yasuyuki
Y
Yokosawa
Hideyoshi
H
D000818:Animals; D001120:Arginine; D015153:Blotting, Western; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D018882:Heat Stress Disorders; D018869:Heat-Shock Response / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008239:Lysine; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D051379:Mice; D008968:Molecular Conformation; D009154:Mutation / Q000187:drug effects; D041681:NIH 3T3 Cells; D025821:Polyubiquitin / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D025821:Polyubiquitin; D007544:Isopropyl Thiogalactoside; D000431:Ethanol; D001120:Arginine; D008741:Methyl Methanesulfonate; D006861:Hydrogen Peroxide; D008239:Lysine