[Molecular characterization of Brucella abortus strain 19 and its application for controlling biologics].
Brucella abortus is the etiological agent of bovine brucellosis. The strain 19 used in vaccine elaboration can be identified through a deletion in the eri region associated with its susceptibility to erythritol. We optimized a PCR assay for specific characterization of this strain. The method described here is a rapid procedure that enables identification of B. abortus, and simultaneous differentiation of the strain 19 from other B. abortus biovar 1 strains. We applied the assay to detect the strain 19 in vaccines against B. abortus produced in Argentina. Thq results show this method could be used to follow vaccine seed cultures of this strain. The methodology could also contribute to reduce the risk of a laboratory-acquired infection and could be of great help as a routine test for confirmation of B. abortus in non related vaccines.
Pavan
M E
ME
Biochemiq S.A. Viamonte 1365 piso 9, Ciudad Autónoma de Buenos Aires, Argentina. mpavan@biochemiq.com
Nicola
A
A
Grimoldi
F
F
Cairó
F
F
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000379:methods*; D002004:Brucella Vaccine; D002003:Brucella abortus / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D002007:Brucellosis, Bovine / Q000382:microbiology*; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004896:Erythritol / Q000378:metabolism; D015345:Oligonucleotide Probes; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*
D001426:Bacterial Proteins; D002004:Brucella Vaccine; D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D017853:Phosphotransferases (Alcohol Group Acceptor); C478753:erythritol kinase; D004896:Erythritol