Trypanocidal activity of oleoresin and terpenoids isolated from Pinus oocarpa.
Fractionation with n-hexane/ethyl acetate (1:1 v/v) by open column chromatography of the oleoresin from Pinus oocarpa Schiede yielded two diterpenes, pimaric acid (1) and dehydroabietic acid (5), the sesquiterpene longifolene (3) and a diterpenic mixture containing pimaric acid (1), isopimaric acid (4) and dehydroabietic acid (5). Subsequently, the isolated compounds, the mixture of 1, 4 and 5, the oleoresin and the dehydroabietic acid methyl ester (2), were tested in vitro against epimastigotes of Trypanosoma cruzi, the causative agent of Chagas disease. The most active compounds were 1, 3 and the oleoresin, being as active as nifurtimox, a drug effective in the treatment of acute infection by American trypanosomiasis and used in this work as positive control.
Rubio
Julieta
J
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México. juruli@servidor.unam.mx
Calderón
José S
JS
Flores
Angélica
A
Castroa
Clementina
C
Céspedes
Carlos L
CL
D000818:Animals; D014355:Chagas Disease; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D009547:Nifurtimox / Q000494:pharmacology; D008517:Phytotherapy; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology; D012717:Sesquiterpenes / Q000494:pharmacology; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014344:Trypanocidal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects*
D004224:Diterpenes; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; D014344:Trypanocidal Agents; C018620:oleoresins; C035607:longifolene; C008911:pimaric acid; D009547:Nifurtimox