Identification of "Candidatus Thioturbo danicus," a microaerophilic bacterium that builds conspicuous veils on sulfidic sediments.
Molecular analysis of bacteria enriched under in situ-like conditions and mechanically isolated by micromanipulation showed that a hitherto-uncultivated microaerophilic bacterium thriving in oxygen-sulfide counter-gradients (R. Thar and M. Kühl, Appl. Environ. Microbiol. 68:6310-6320, 2000) is affiliated with the epsilon-subdivision of the Proteobacteria. The affiliation was confirmed by the use of whole-cell hybridization with newly designed specific oligonucleotide probes. The bacterium belongs to a new genus and received the provisional name "Candidatus Thioturbo danicus."
Muyzer
Gerard
G
Department of Biotechnology, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, The Netherlands. g.muyzer@tnw.tudelft.nl
Yildirim
Esengül
E
van Dongen
Udo
U
Kühl
Michael
M
Thar
Roland
R
D001420:Bacteria, Aerobic / Q000145:classification / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D003718:Denmark; D019015:Geologic Sediments / Q000032:analysis / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013440:Sulfides / Q000378:metabolism*
D017931:DNA Primers; D013440:Sulfides