title: Dendritic BC1 RNA in translational control mechanisms.
abstract: Translational control at the synapse is thought to be a key determinant of neuronal plasticity. How is such control implemented? We report that small untranslated BC1 RNA is a specific effector of translational control both in vitro and in vivo. BC1 RNA, expressed in neurons and germ cells, inhibits a rate-limiting step in the assembly of translation initiation complexes. A translational repression element is contained within the unique 3' domain of BC1 RNA. Interactions of this domain with eukaryotic initiation factor 4A and poly(A) binding protein mediate repression, indicating that the 3' BC1 domain targets a functional interaction between these factors. In contrast, interactions of BC1 RNA with the fragile X mental retardation protein could not be documented. Thus, BC1 RNA modulates translation-dependent processes in neurons and germs cells by directly interacting with translation initiation factors.
authors.affiliation: Department of Physiology and Pharmacology, State University of New York, Health Science Center at Brooklyn, Brooklyn, NY 11203, USA.
authors.forename: Huidong
authors.initials: H
authors.lastname: Wang
authors.forename: Anna
authors.initials: A
authors.lastname: Iacoangeli
authors.forename: Daisy
authors.initials: D
authors.lastname: Lin
authors.forename: Keith
authors.initials: K
authors.lastname: Williams
authors.forename: Robert B
authors.initials: RB
authors.lastname: Denman
authors.forename: Christopher U T
authors.initials: CU
authors.lastname: Hellen
authors.forename: Henri
authors.initials: H
authors.lastname: Tiedge
mesh_terms: D024509:3' Flanking Region; D000818:Animals; D003712:Dendrites / Q000378:metabolism*; D039601:Eukaryotic Initiation Factor-4A / Q000235:genetics / Q000378:metabolism; D005260:Female; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D009865:Oocytes / Q000378:metabolism; D039102:Poly(A)-Binding Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism; D020733:RNA, Small Cytoplasmic / Q000235:genetics / Q000502:physiology*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D039601:Eukaryotic Initiation Factor-4A; D051860:Fragile X Messenger Ribonucleoprotein 1; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; D020733:RNA, Small Cytoplasmic; C117734:BC1 RNA