title: Bone marrow trephine biopsy findings in chronic myeloid leukemia.
abstract: Sixty patients with chronic myeloid leukemia (CML) underwent bone marrow trephine biopsy at presentation. All the biopsies were decalcified, paraffin-embedded and stained with H&E, Gomori's reticulin and Masson Trichrome. A detailed study of the histology including the morphology and topographic distribution of megakaryocytes was done. 55 patients presented in chronic phase. Of these there were 37 cases (67%) of CML-granulocytic (CML-G) type and 18 cases (33%) of CML-granulocytic megakaryocytic (CML -GM) type. Five cases presented in blast crisis. 73% of CML-G had low-grade fibrosis while 83% of CML-GM had high-grade fibrosis. This was statistically significant. On follow-up 25% of CML-G went into blast crisis while all the CML-GM patients remained stable to date. Bone marrow biopsy is a useful investigation in patients of CML at diagnosis as it provides prognostic information. Evaluation of megakaryopoiesis, grading of fibrosis and localization of blasts are possible on a trephine biopsy.
authors.affiliation: Department of Pathology, Jawaharlal Institute of Post Graduate Medical Education and Research, Pondicherry, India.
authors.forename: Yookarin
authors.initials: Y
authors.lastname: Khonglah
authors.forename: Debdatta
authors.initials: D
authors.lastname: Basu
authors.forename: Tarun Kumar
authors.initials: TK
authors.lastname: Dutta
mesh_terms: D001706:Biopsy; D001752:Blast Crisis / Q000209:etiology / Q000473:pathology; D001854:Bone Marrow Cells / Q000473:pathology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000145:classification / Q000150:complications / Q000473:pathology*; D008533:Megakaryocytes / Q000473:pathology; D055728:Primary Myelofibrosis / Q000150:complications / Q000473:pathology