Linkage analysis of chromosome 14 and essential hypertension in Chinese population.
BACKGROUND
Hypertension is a complex biological trait that influenced by multiple factors. The encouraging results for hypertension research showed that the linkage analysis can be used to replicate other studies and discover new genetic risk factors. Previous studies linked human chromosome 14 to essential hypertension or blood pressure traits. With a Chinese population, we tried to replicate these findings.


METHODS
A linkage scan was performed on chromosome 14 with 14-microsatellite markers with a density of about 10 centi Morgen (cM) in 147 Chinese hypertensive nuclear families. Multipoint non-parametric linkage analysis and exclusion mapping were performed with the GENEHUNTER software, whereas quantitative analysis was performed with the variance component method integrated in the SOLAR package.


RESULTS
In the qualitative analysis, the highest non-parametric linkage score is 1.0 (P = 0.14) at D14S261 in the single point analysis, and no loci achieved non-parametric linkage score more than 1.0 in the multipoint analysis. Maximum-likelihood mapping showed no significant results, either. Subsequently the traditional exclusion criteria of the log-of-the-odds score-2 were adopted, and the chromosome 14 with lambda s > or = 2.4 was excluded. In the quantitative analysis of blood pressure with the SOLAR software, two-point analysis and multipoint analysis suggested no evidence for linkage occurred on chromosome 14 for systolic and diastolic blood pressure.


CONCLUSIONS
There was no substantial evidence to support the linkage of chromosome 14 and essential hypertension or blood pressure trait in Chinese hypertensive subjects in this study.
Zhao
Wei-yan
WY
Division of Population Genetics and Prevention, Cardiovascular Institute, Fuwai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100037, China.
Huang
Jian-feng
JF
Ge
Dong-liang
DL
Su
Shao-yong
SY
Li
Biao
B
Gu
Dong-feng
DF
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D002883:Chromosomes, Human, Pair 14; D008040:Genetic Linkage; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008126:Lod Score; D008875:Middle Aged