[Quantitative study on C/EBP zeta gene transcripts in patients with chronic myeloid leukemia using real-time quantitative PCR].
OBJECTIVE
To evaluate the significance of quantitative detection of CCAAT/enhancer binding protein zeta (C/EBP zeta) transcripts in patients with chronic myeloid leukemia (CML).


METHODS
Real-time quantitative polymerase chain reaction (RQ-PCR) assay was established and performed to measure the transcript level of C/EBP zeta in the bone marrow mononuclear cells from 76 patients with CML at different stages.


RESULTS
C/EBP zeta transcripts were significantly decreased in CML patients in chronic phage, accelerated phage, and blastic crisis (median 2.5, 3.31, and 2.22, respectively), compared to that in normal controls (median 12.20). Further down-regulation of C/EBP zeta was observed in the patients resistant to interferon (median 1.56); however, its expression level returned to normal in the patients who obtained complete cytogenetic remission (median 15.43).


CONCLUSIONS
C/EBP zeta was down-regulated in CML patients, which might play a role in the leukemogenesis.
Qian
Jun
J
Department of Hematology, Renmin Affiliated Hospital, Jiangsu University, Zhenjiang, Jiangsu, P. R. China.
Chen
Zi-xing
ZX
Lin
Jiang
J
Hu
Shao-yan
SY
Cen
Jian-nong
JN
Wang
Wei
W
D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014158:Transcription, Genetic
D022762:CCAAT-Enhancer-Binding Proteins