The genome project of Taenia solium.
We have constituted a consortium of key laboratories at the National Autonomous University of Mexico to carry out a genomic project for Taenia solium. This project will provide powerful resources for the study of taeniasis/cysticercosis, and, in conjunction with the Echinococcus granulosus and Echinococcus multilocularis genome project of expressed sequence tags (ESTs), will mark the advent of genomics for cestode parasites. Our project is planned in two consecutive stages. The first stage is being carried out to determine some basic parameters of the T. solium genome. Afterwards, we will evaluate the best strategy for the second stage, a full blown genome project. We have estimated the T. solium genome size by two different approaches: cytofluorometry on isolated cyton nuclei, as well as a probabilistic calculation based on approximately 2000 sequenced genomic clones, approximately 3000 ESTs, resulting in size estimates of 270 and 251 Mb, respectively. In terms of sequencing, our goal for the first stage is to characterize several thousand EST's (from adult worm and cysticerci cDNA libraries) and genomic clones. Results obtained so far from about 16,000 sequenced ESTs from the adult stage, show that only about 40% of the T. solium coding sequences have a previously sequenced homologue. Many of the best hits are found with mammalian genes, especially with humans. However, 1.5% of the hits lack homologues in humans, making these genes immediate candidates for investigation on pharmaco-therapy, diagnostics and vaccination. Most T. solium ESTs are related to gene regulation, and signal transduction. Other important functions are housekeeping, metabolism, cell division, cytoskeleton, proteases, vacuolar transport, hormone response, and extracellular matrix activities. Preliminary results also suggest that the genome of T. solium is not highly repetitive.
Aguilar-Díaz
Hugo
H
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico.
Bobes
Raúl J
RJ
Carrero
Julio C
JC
Camacho-Carranza
Rafael
R
Cervantes
Claudia
C
Cevallos
Miguel A
MA
Dávila
Guillermo
G
Rodríguez-Dorantes
Mauricio
M
Escobedo
Galileo
G
Fernández
José Luis
JL
Fragoso
Gladis
G
Gaytán
Paul
P
Garciarubio
Alejandro
A
González
Victor M
VM
González
Lorena
L
José
Marco V
MV
Jiménez
Lucía
L
Laclette
Juan P
JP
Landa
Abraham
A
Larralde
Carlos
C
Morales-Montor
Jorge
J
Morett
Enrique
E
Ostoa-Saloma
Pedro
P
Sciutto
Edda
E
Santamaría
Rosa I
RI
Soberón
Xavier
X
de la Torre
Patricia
P
Valdés
Víctor
V
Yánez
Jorge
J
D000818:Animals; D003551:Cysticercosis / Q000469:parasitology; D003552:Cysticercus; D049751:Genome, Helminth; D023281:Genomics; D006801:Humans; D041201:Taenia solium / Q000235:genetics* / Q000254:growth & development