Simple and rapid method for the isolation of forskolin from Coleus forskohlii by charcoal column chromatography.
A simple, safe, rapid and economical method was developed for the isolation of high-purity forskolin from Coleus forskohlii roots using activated charcoal as an adsorbent in a column. The elution was carried out under reduced pressure to make the process rapid. Activated charcoal acted as a reversed phase adsorbent and allowed elution of forskolin without much impurities. The residue, obtained from the eluate was purified and crystallized using different solvent mixtures to obtain pure forskolin. The forskolin isolated was analyzed and characterized by UV, IR, RP-HPLC, electrospray ionization MS, 1H NMR and 13C NMR. The yield was 0.097% w/w (RSD 5.6%). The purity was 96.9% w/w (RSD 0.3%) as determined by RP-HPLC. The present method enables researchers to produce high-purity forskolin in their labs by using common chemicals.
Saleem
A M
AM
Department of Pharmacognosy, C.L. Baid Metha College of Pharmacy, Thoraippakkam, Chennai 600096, India. saleemskma@rediffmail.com
Dhasan
P B
PB
Rafiullah
M R M
MR
D000327:Adsorption; D002606:Charcoal; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer; D031330:Coleus / Q000737:chemistry*; D005576:Colforsin / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry*
D002606:Charcoal; D005576:Colforsin