title: Mrg19 depletion increases S. cerevisiae lifespan by augmenting ROS defence.
abstract: Caloric restriction (CR) is the most compelling example of lifespan extension by external manipulation. Although the molecular mechanisms remain unknown, the theory of hormesis has been invoked to explain the life promoting effects of CR. Hormesis is defined as the beneficial effects of low intensity stressor on a cell or organism. Mrg19 is a putative transcription factor that regulates carbon and nitrogen metabolism in yeast. In this study, we have found that deletion of MRG19 gene causes metabolic shift in yeast cells, leading to higher intracellular reactive oxygen species, augmentation of scavenging enzymes and longer lifespan compared to wild-type cells. All these results together suggest that similar to CR, depletion of Mrg19 leads to a condition of mild stress which in turn enhances vitality.
authors.affiliation: Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Sector 67, S.A.S. Nagar, Punjab 160062, India.
authors.forename: Sujay V
authors.initials: SV
authors.lastname: Kharade
authors.forename: Nitish
authors.initials: N
authors.lastname: Mittal
authors.forename: Shankar P
authors.initials: SP
authors.lastname: Das
authors.forename: Pratima
authors.initials: P
authors.lastname: Sinha
authors.forename: Nilanjan
authors.initials: N
authors.lastname: Roy
mesh_terms: D031204:Caloric Restriction; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D005800:Genes, Fungal; D008136:Longevity; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption; D017382:Reactive Oxygen Species; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; C497794:MRG19 protein, S cerevisiae; D017382:Reactive Oxygen Species; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen