Development of cat embryos produced by intracytoplasmic injection of spermatozoa stored in alcohol.
This study was conducted to examine whether cat spermatozoa stored in ethanol for 1 month was capable of developing into pronuclei and of supporting normal embryonic development. In vitro matured oocytes were injected with frozen-thawed spermatozoa and ethanol-stored spermatozoa. The status of oocytes and sperm nuclei was examined at 4 and 18 h after injection of spermatozoa, and the presumptive zygotes were cultured for 7 days to assess the development of oocytes injected with each storage sperm. The percentage of enlarged sperm head at 4 h after injection was higher in ethanol-stored spermatozoa than in frozen-thawed spermatozoa, but there was no significant difference in the development of oocytes and sperm nuclei at 18 h after injection between the two groups. The development of oocytes to the blastocyst stage after injection of spermatozoa was observed only in oocytes with frozen-thawed spermatozoa. Of oocytes injected with ethanol-stored spermatozoa, two (2.8%) oocytes developed to the 16-cell stage. These results indicate that cat spermatozoa stored in ethanol can decondense and form male pronuclei after intracytoplasmic injection. However, the oocytes injected with ethanol-stored spermatozoa did not have the ability to develop to the blastocyst stage.
Murakami
M
M
Laboratory of Animal Reproduction, Department of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
Karja
N W K
NW
Wongsrikeao
P
P
Agung
B
B
Taniguchi
M
M
Naoi
H
H
Otoi
T
T
D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002415:Cats / Q000196:embryology* / Q000502:physiology; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D011247:Pregnancy; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000502:physiology
D000431:Ethanol