The substrate-induced conformational change of Mycobacterium tuberculosis mycothiol synthase.
The structure of the ternary complex of mycothiol synthase from Mycobacterium tuberculosis with bound desacetylmycothiol and CoA was determined to 1.8 A resolution. The structure of the acetyl-CoA-binary complex had shown an active site groove that was several times larger than its substrate. The structure of the ternary complex reveals that mycothiol synthase undergoes a large conformational change in which the two acetyltransferase domains are brought together through shared interactions with the functional groups of desacetylmycothiol, thereby decreasing the size of this large central groove. A comparison of the binary and ternary structures illustrates many of the features that promote catalysis. Desacetylmycothiol is positioned with its primary amine in close proximity and in the proper orientation for direct nucleophilic attack on the si-face of the acetyl group of acetyl-CoA. Glu-234 and Tyr-294 are positioned to act as a general base and general acid to promote acetyl transfer. In addition, this structure provides further evidence that the N-terminal acetyltransferase domain no longer has enzymatic activity and is vestigial in nature.
Vetting
Matthew W
MW
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461-1602, USA.
Yu
Michael
M
Rendle
Phillip M
PM
Blanchard
John S
JS
D000123:Acetyltransferases / Q000737:chemistry*; D001665:Binding Sites; D003065:Coenzyme A / Q000737:chemistry; D018360:Crystallography, X-Ray; D003545:Cysteine; D004187:Disaccharides / Q000737:chemistry; D006020:Glycopeptides; D007294:Inositol; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D011485:Protein Binding; D011487:Protein Conformation; D011720:Pyrazoles / Q000737:chemistry; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry
D004187:Disaccharides; D006020:Glycopeptides; D011720:Pyrazoles; D013438:Sulfhydryl Compounds; C089265:mycothiol; D007294:Inositol; D000123:Acetyltransferases; C470312:acetyl-CoA 1-D-myo-inosityl 2-L-cysteinylamido-2-deoxy-alpha-D-glucopyranoside acetyltransferase; D003545:Cysteine; D003065:Coenzyme A