title: Anticlastogenic effects of black tea polyphenols theaflavins and thearubigins in human lymphocytes in vitro.
abstract: Black tea accounts for nearly 80% of total World tea production. It contains dimeric flavanols and polymeric polyphenols known as theaflavins (TF) and thearubigins (TR). TR is exclusively present in black tea. On the basis of our previous potent antimutagenic and anticlastogenic effects of TF and TR in vitro in bacterial system and in vivo in mouse bone marrow cells, we have decided to extend our study in human cells in vitro. This study investigated the anticlastogenic effects of black tea polyphenols TF and TR as measured by chromosomal aberrations (CA) and micronuclei formation (MN) against two known mutagens/carcinogens i.e. benzo[a]pyerne (B[a]P) and aflatoxin B1(AFB1) with S9 activation. A significant decrease in both CA and MN were observed in the human lymphocyte cultures treated with either TF or TR pretreated with either B[a]P or AFB1 (250, 500, 1000 microg/ml) when compared with B[a]P or AFB1 treated cultures alone. TF shows more protective effects than TR in this in vitro system. These results indicate that both TF and TR have significant anticlastogenic effects in vitro in human lymphocytes.
authors.affiliation: Division of Human Genetics and Genomics, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Jadavpur, Kolkata 700,032, West Bengal, India.
authors.forename: Babli
authors.initials: B
authors.lastname: Halder
authors.forename: Swapan
authors.initials: S
authors.lastname: Pramanick
authors.forename: Sibabrata
authors.initials: S
authors.lastname: Mukhopadhyay
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Giri
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D044946:Biflavonoids / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D013662:Tea
chemical_list: D016587:Antimutagenic Agents; D044946:Biflavonoids; D010636:Phenols; D059808:Polyphenols; D013662:Tea; C086701:thearubigin; C056068:theaflavin; D002392:Catechin