title: The molecular epidemiology of Mycobacterium bovis infections in Tanzania.
abstract: A molecular epidemiological study to determine the zoonotic importance of bovine tuberculosis was carried out in Tanzania. Specimens from human cases of tuberculosis as well as from slaughtered cattle were collected from regions with a high proportion of extrapulmonary tuberculosis. In order to determine the similarity of strains from the two sources, molecular typing techniques, namely RFLP and spoligotyping, were used to determine the genetic profile of the strains involved. The results of pTBN12 typing of M. bovis from cattle and man has shown a rather heterogeneous population of this species spread all over Tanzania, assuming that the present sample is representative. There were 13 different pTBN12 RFLP types encountered. The genetic relatedness between the pTBN12 RFLP patterns indicated a high degree of relatedness (86%) between the dominant pTBN12 genotypes existing in Tanzania. There were 13 different spoligotypes found in this study, whose genetic relatedness was also high (79%). DNA profiles were also confirmed by IS986 RFLP, which revealed that strains have 1-13 copies of IS986. Geographically, there was overlap between pTBN12 RFLP and spoligotypes amongst strains isolated from various parts of Tanzania. The diversity of the RFLP and spoligotype patterns observed in Tanzania probably reflects the extensive internal movements of cattle belonging to pastoralists. The evidence of overlap between DNA fingerprints of M. bovis from cattle and man has once more highlighted a need for synergy of veterinary and medical policies in the control of tuberculosis in Tanzania and probably in other developing countries.
authors.affiliation: Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, P.O. Box 3021, Morogoro, Tanzania. kazwala@suanet.ac.tz
authors.forename: R R
authors.initials: RR
authors.lastname: Kazwala
authors.forename: L J M
authors.initials: LJ
authors.lastname: Kusiluka
authors.forename: K
authors.initials: K
authors.lastname: Sinclair
authors.forename: J M
authors.initials: JM
authors.lastname: Sharp
authors.forename: C J
authors.initials: CJ
authors.lastname: Daborn
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods; D002417:Cattle; D016172:DNA Fingerprinting / Q000379:methods; D003710:Demography; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D013636:Tanzania / Q000453:epidemiology; D014380:Tuberculosis, Bovine / Q000453:epidemiology* / Q000382:microbiology; D015047:Zoonoses