title: Hydrodistillation-headspace solvent microextraction, a new method for analysis of the essential oil components of Lavandula angustifolia Mill.
abstract: A new method involving concurrent headspace solvent microextraction combined with continuous hydrodistillation (HD-HSME) for the extraction and pre-concentration of the essential oil of Lavandula angustifolia Mill. into a microdrop is developed. A microdrop of n-hexadecane containing n-heptadecane (as internal standard) extruded from the needle tip of a gas chromatographic syringe was inserted into the headspace above the plant sample. After extraction for an optimized time, the microdrop was retracted into the syringe and injected directly into a GC injection port. The effects of the type of extracting solvent, sample mass, microdrop volume and extraction time on HD-HSME efficiency were investigated and optimized. Using this method, thirty-six compounds were extracted and identified. Linalool (32.8%), linalyl acetate (17.6%), lavandulyl acetate (15.9%), alpha-terpineol (6.7%) and geranyl acetate (5.0%) were found to be the major constituents. To the best of our knowledge this is the first report on the use of continuous headspace solvent microextraction coupled with hydrodistillation for investigation of essential oil components.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Shahid Beheshti University, P.O. Box 19835-389, Evin, Tehran, Iran. Alireza.FakhariZavareh@utas.edu.au
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Fakhari
authors.forename: Peyman
authors.initials: P
authors.lastname: Salehi
authors.forename: Rouhollah
authors.initials: R
authors.lastname: Heydari
authors.forename: Samad Nejad
authors.initials: SN
authors.lastname: Ebrahimi
authors.forename: Paul R
authors.initials: PR
authors.lastname: Haddad
mesh_terms: D000085:Acetates / Q000737:chemistry / Q000302:isolation & purification; D000080462:Acyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D005504:Food Analysis / Q000379:methods*; D027523:Lavandula / Q000737:chemistry*; D008832:Microchemistry; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D000085:Acetates; D000080462:Acyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D012997:Solvents; D013729:Terpenes; C016775:alpha-terpineol; C432872:geranyl acetate; C074463:linalyl acetate; C018584:linalool; C492922:lavandulyl acetate