Plasmids for nicotine-dependent and -independent gene expression in Arthrobacter nicotinovorans and other arthrobacter species.
The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow the production of complex enzymes and genetic complementation studies in Arthrobacter species.
Sandu
Cristinel
C
Institute of Biochemistry and Molecular Biology, University of Freiburg, Hermann Herder Str. 7, 79104 Freiburg, Germany. sanduc@rockefeller.edu
Chiribau
Calin-Bogdan
CB
Sachelaru
Paula
P
Brandsch
Roderich
R
D001173:Arthrobacter / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009538:Nicotine / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects / Q000235:genetics*; D015183:Restriction Mapping
D049452:Green Fluorescent Proteins; D009538:Nicotine