Cell adsorption and selective desorption for separation of microbial cells by using chitosan-immobilized silica.
Cell adsorption and selective desorption for separation of microbial cells were conducted by using chitosan-immobilized silica (CIS). When chitosan was immobilized onto silica surfaces with glutaraldehyde, bacterial cells adsorbed well and retained viability. Testing of the adsorption and desorption ability of CIS using various microbes such as Escherichia coli, Aeromonas hydrophila, Pseudomonas aeruginosa, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus casei, Streptococcus mutans, Streptococcus sobrinus, Streptococcus salivarius, Saccharomyces cerevisiae, Saccharomyces ludwigii, and Schizosaccharomyces pombe revealed that most microbes could be adsorbed and selectively desorbed under different conditions. In particular, recovery was improved when L-cysteine was added. A mixture of two bacterial strains adsorbed onto CIS could also be successfully separated by use of specific solutions for each strain. Most of the desorbed cells were alive. Thus, quantitative and selective fractionation of cells is readily achievable by employing chitosan, a known antibacterial material.
Kubota
Munehiro
M
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamzaki, Noda-shi, Chiba-ken 278-8510, Japan.
Matsui
Masayoshi
M
Chiku
Hiroyuki
H
Kasashima
Nobuyuki
N
Shimojoh
Manabu
M
Sakaguchi
Kengo
K
D000327:Adsorption; D001412:Bacillus subtilis / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D002470:Cell Survival; D048271:Chitosan; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification; D005658:Fungi / Q000145:classification / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification; D012685:Sepharose / Q000031:analogs & derivatives
C014611:immobilized amphipathic ampholytes; D012685:Sepharose; D048271:Chitosan