Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus.
The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes have sufficient substrate promiscuity to permit the metabolism of both sugars. Recently, it has been proposed that the part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus as an alternative route for glucose metabolism. In this report we demonstrate, by in vitro kinetic studies of D-2-keto-3-deoxygluconate (KDG) kinase and KDG aldolase, that the part-phosphorylative pathway in S. solfataricus is also promiscuous for the metabolism of both glucose and galactose.
Lamble
Henry J
HJ
Centre for Extremophile Research, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
Theodossis
Alex
A
Milburn
Christine C
CC
Taylor
Garry L
GL
Bull
Steven D
SD
Hough
David W
DW
Danson
Michael J
MJ
D000255:Adenosine Triphosphate / Q000378:metabolism; D000446:Aldehyde-Lyases / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001105:Archaea / Q000378:metabolism*; D001711:Biotransformation; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005690:Galactose / Q000378:metabolism; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D048229:Sulfolobus solfataricus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
D010455:Peptides; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C021471:2-keto-3-deoxygluconokinase; D000446:Aldehyde-Lyases; C021468:2-keto-3-deoxy-D-glucarate aldolase; D005947:Glucose; D005690:Galactose