Bioavailability assessment and environmental fate of polycyclic aromatic hydrocarbons in biostimulated creosote-contaminated soil.
When hydrocarbon-contaminated soil is subjected to bioremediation technology, hydrocarbon depletion is typically marked by an initially rapid reduction rate. This rate decreases over time and frequently a residual concentration remains in the soil. This kinetic has been attributed primarily to the enrichment of more recalcitrant fractions, as well as to the lack of resting hydrocarbon bioavailability. Thus, at the end of the bioremediation process, a part of the residual hydrocarbon soil concentration represents the non-bioavailable fraction, which is difficult to degrade by microbial populations and which poses a minor hazard. Therefore, determination of the bioavailable fraction in a bioremediation project represents both an estimation of the maximum level of achievable biodegradation, as well as an additional indication of the environmental health hazard. In the present study, aged creosote-contaminated soil was subjected to biostimulation processes, and the bioavailable fraction for several target polycyclic aromatic hydrocarbons (PAHs) was calculated using a mild extraction with cyclodextrines. The amount of PAH extracted corresponded to the desorbing fraction and can be regarded as the bioavailable fraction. The non-desorbing fraction data obtained from this procedure were compared to the remaining PAH concentrations following bioremediation treatment of soil microcosms. These results permitted the establishment of a theoretical biodegradation limit based on the desorbing fraction. In addition, neither accumulation of intermediate metabolites, nor the formation of bound-residues or reduced acute toxicity was observed.
Sabaté
Jordi
J
Department of Microbiology, University of Barcelona, Spain.
Viñas
Marc
M
Solanas
Anna M
AM
D000327:Adsorption; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism; D001673:Biodegradation, Environmental; D003407:Creosote; D003505:Cyclodextrins / Q000737:chemistry; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity
D003505:Cyclodextrins; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D003407:Creosote