title: A novel protein refolding method using a zeolite.
abstract: We have succeeded in developing a simple and effective protein refolding method using the inorganic catalyst, beta-zeolite. The method involves the adsorption of proteins solubilized with 6M guanidine hydrochloride from inclusion body (IB) preparations onto the zeolite. The denaturant is then removed, and the proteins in the IBs are released from the zeolite with polyoxyethylene detergent and salt. All of the IBs tested (11 different species) were successfully refolded under these conditions. The refolded proteins are biochemically active, and NMR analysis of one of the proteins (replication protein A 8) supports the conclusion that correct refolding does occur. Based on these results, we discuss the refolding mechanism.
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda-shi, Chiba-ken 278-8510, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Chiku
authors.forename: Akiko
authors.initials: A
authors.lastname: Kawai
authors.forename: Toyotaka
authors.initials: T
authors.lastname: Ishibashi
authors.forename: Masahide
authors.initials: M
authors.lastname: Takehara
authors.forename: Takuro
authors.initials: T
authors.lastname: Yanai
authors.forename: Fujio
authors.initials: F
authors.lastname: Mizukami
authors.forename: Kengo
authors.initials: K
authors.lastname: Sakaguchi
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000378:metabolism; D019791:Guanidine / Q000737:chemistry; D002479:Inclusion Bodies / Q000737:chemistry*; D017510:Protein Folding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D051716:Replication Protein A / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D017641:Zeolites / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; D051716:Replication Protein A; D017641:Zeolites; D019791:Guanidine