Effect of SsrA (tmRNA) tagging system on translational regulation in Streptomyces.
ssrA genes encoding tmRNA with transfer and messenger RNA functions are ubiquitous in bacteria. In a process called trans-translation, tmRNA enters a stalled ribosome and allows release of the original mRNA, then tmRNA becomes the template for translation of a short tag that signals for proteolytic degradation. We provide here the first evidences that the tmRNA tagging system (ssrA and cohort smpB) is active in Streptomyces. Transcription of the genes was shown and construction of a genetic probe allowed detection of a tmRNA-tagged peptide. Obtention of ssrA and smpB mutants of Streptomyces lividans showed that the ssrA system is dispensable in Streptomyces. Morphologies of the mutants colonies were similar to the wild type, thus tmRNA-mediated tagging does not seem to have, under conditions used, a significant effect in the Streptomyces differentiation.
Braud
Sandrine
S
UP Génétique Bactérienne et Différenciation, Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France.
Lavire
Celine
C
Bellier
Audrey
A
Mazodier
Philippe
P
D001426:Bacterial Proteins / Q000502:physiology; D010450:Endopeptidases / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D014176:Protein Biosynthesis / Q000502:physiology*; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000502:physiology*; D048372:Streptomyces coelicolor / Q000502:physiology; D048170:Streptomyces lividans / Q000502:physiology
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; C096222:tmRNA; D012343:RNA, Transfer; D010450:Endopeptidases; C072135:C-terminal processing peptidase