Coated-platelets retain amyloid precursor protein on their surface.
Coated-Platelets are a subset of platelets produced by dual-agonist activation with collagen plus thrombin and are characterized by strong retention of several procoagulant, alpha-granule proteins on the cell surface. In this report we demonstrate that coated-platelets also retain full-length amyloid precursor protein (APP) on their surface in contrast to the cleavage of APP in platelets activated with a single agonist. In addition, western blot analysis indicated that APP is derivatized during coated-platelet synthesis. We subsequently measured coated-platelet production in patients with Alzheimer's disease (AD). Twenty-two AD patients showed a wide distribution of coated-platelet values; however the least impaired AD patients produced coated-platelets at a level significantly above that of aged controls (41.0 +/- 9.9 vs. 28.7 +/- 11.4%; mean +/- 1SD; p = 0.017). These findings suggest that coated-platelets may be a model of aberrant APP processing in early AD patients.
Prodan
Calin I
CI
Department of Neurology, University of Oklahoma Health Sciences Center, klahoma City, Oklahoma 73104, USA.
Szasz
Robert
R
Vincent
Andrea S
AS
Ross
Elliott D
ED
Dale
George L
GL
D000367:Age Factors; D000544:Alzheimer Disease / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D001792:Blood Platelets / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D015153:Blotting, Western; D003435:Crotalid Venoms / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D037181:Lectins, C-Type; D008297:Male; D008970:Molecular Weight; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology; D013499:Surface Properties; D013917:Thrombin / Q000494:pharmacology
D016564:Amyloid beta-Protein Precursor; D003435:Crotalid Venoms; D037181:Lectins, C-Type; C028890:convulxin; D013917:Thrombin