title: Production of vanillin by metabolically engineered Escherichia coli.
abstract: E. coli was metabolically engineered to produce vanillin by expression of the fcs and ech genes from Amycolatopsis sp. encoding feruloyl-CoA synthetase and enoyl-CoA hydratase/aldolase, respectively. Vanillin production was optimized by leaky expression of the genes, under the IPTG-inducible trc promoter, in complex 2YT medium. Supplementation with glucose, fructose, galactose, arabinose or glycerol severely decreased vanillin production. The highest vanillin production of 1.1 g l(-1) was obtained with cultivation for 48 h in 2YT medium with 0.2% (w/v) ferulate, without IPTG and no supplementation of carbon sources.
authors.affiliation: Department of Food Science & Nutrition, Gyeongsang National University, 660-701, Jinju, Korea.
authors.forename: Sang-Hwal
authors.initials: SH
authors.lastname: Yoon
authors.forename: Cui
authors.initials: C
authors.lastname: Li
authors.forename: Ju-Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Sook-Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Yoon
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Choi
authors.forename: Weon-Taek
authors.initials: WT
authors.lastname: Seo
authors.forename: Jae-Kyung
authors.initials: JK
authors.lastname: Yang
authors.forename: Jae-Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Seon-Won
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000192:Actinomycetales / Q000235:genetics* / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D001547:Benzaldehydes / Q000302:isolation & purification* / Q000378:metabolism*; D004746:Enoyl-CoA Hydratase / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*
chemical_list: D001547:Benzaldehydes; C100058:vanillin; D000445:Aldehyde Oxidoreductases; C023101:feruloyl-coenzyme A reductase; D004746:Enoyl-CoA Hydratase