title: Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens.
abstract: Severe acute respiratory syndrome (SARS) is a recently discovered viral disease, characterized by fever, cough, acute fibrinous pneumonia and high infectivity. Specific pathogen-free (SPF) chickens were immunized with inactivated SARS coronavirus and their eggs were harvested at regular intervals. Yolk immunoglobulin (IgY) was extracted using the water dilution method, followed by further purification on a Sephadex G-75 column. SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and neutralization test results showed that the IgY obtained was of a high purity and had a strong reactive activity with a neutralization titer of 1:640. Lyophilization and stability tests showed that lyophilized anti-SARS coronavirus IgY had promising physical properties, with no significant reduction in reactive activity and good thermal stability. All these data suggest that the anti-SARS coronavirus IgY could be a new useful biological product for specific antiviral therapy against SARS.
authors.affiliation: National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Chao-Yang
authors.initials: CY
authors.lastname: Fu
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Wang
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Liu
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Wang
authors.forename: Shang-Jin
authors.initials: SJ
authors.lastname: Cui
authors.forename: Hong-Lei
authors.initials: HL
authors.lastname: Gao
authors.forename: Zan
authors.initials: Z
authors.lastname: Li
authors.forename: Jing-Peng
authors.initials: JP
authors.lastname: Li
authors.forename: Xian-Gang
authors.initials: XG
authors.lastname: Kong
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens; D004530:Egg Yolk / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005612:Freeze Drying; D007114:Immunization; D007136:Immunoglobulins / Q000276:immunology* / Q000302:isolation & purification; D009500:Neutralization Tests; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000517:prevention & control* / Q000662:veterinary / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors
chemical_list: D000914:Antibodies, Viral; C016512:IgY; D007136:Immunoglobulins