[Study on biological effect of transforming growth factor B and recombinant human bone morphogenetic protein 2 on the Schwann cell].
OBJECTIVE
To study biological effect of transforming growth factor beta (TGF-beta) and recombinant human bone morphogenetic protein 2 (rhBMP-2) on the Schwann cell (SC) in vitro.


METHODS
Cultured SC from newborn SD rats were implanted at 5 x 10(3)/well in 96-well-plate (36 wells in each group, altogether 3 groups): TGF-beta group (group A) treated with 50 ng/ml TGF-beta; rhBMP-2 group (group B) treated with 50 ng/ml rhBMP-2 and control group (group C). SC proliferation activity was assessed by MTT and flow cytometry (FCM) methods, and nerve growth factor (NGF) synthesis in SC culture media was detected by ELISA method.


RESULTS
MTT observation indicated that there was significant difference in the growth curve among 3 groups until the 8th and 9th day. Group A had more obvious rising tendency than group B and group C. FCM observation indicated that the proliferation index of group A and group B was higher than that of group C(P<0.05). ELISA observation indicated that there was significant difference in the NGF concentration of the culture medium among the 3 groups (P<0.05). Group A had the highest NGF concentration.


CONCLUSIONS
Exogenous TGF-beta and rhBMP-2 can promote SC's ability to proliferate NGF, but TGF-beta is more effective than rhBMP-2.
Zhang
Yongjie
Y
Tissue Engineering Center, Department of Oral Histology & Pathology, College of Stomatology, the Fourth Military Medical University, Xi'an Shaanxi, 710032, P. R. China.
Jin
Yan
Y
Nie
Xin
X
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology*; D012583:Schwann Cells / Q000187:drug effects* / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2