title: Genome-wide screening of aluminum tolerance in Saccharomyces cerevisiae.
abstract: Genome-wide screening has identified 37 Al-tolerance genes in Saccharomyces cerevisiae. These genes can be roughly categorised into three groups on the basis of function, i.e., genes related to vesicle transport processes, signal transduction pathways, and protein mannosylation. The largest group is composed of genes related to vesicle transport processes; severe Al sensitivity was found in yeast strains lacking these genes. The retrograde transport of endosome-derived vesicles back to the Golgi apparatus is an important factor in determining the Al tolerance of the vesicle transport system. The PKC1-MAPK cascade signalling pathway is important in the Al tolerance of signal transduction. The lack of the gene implicated in this process leads to weakened cell wall architecture, rendering the yeast Al-sensitive. Alternatively, Al might attack the cell wall and/or plasma membrane, and, as signalling is prevented in cells devoid of the genes related to signalling processes, the cells may be unable to alleviate the damage. The genes for protein mannosylation are also associated with Al tolerance, demonstrating the importance of cell wall architecture. These genes are involved in cell integrity processes.
authors.affiliation: Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kakimoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Ryouichi
authors.initials: R
authors.lastname: Fukuda
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ono
authors.forename: Yasuke
authors.initials: Y
authors.lastname: Ono
authors.forename: Akinori
authors.initials: A
authors.lastname: Ohta
authors.forename: Etsuro
authors.initials: E
authors.lastname: Yoshimura
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D002473:Cell Wall / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D023281:Genomics / Q000379:methods; D008358:Mannose / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013329:Structure-Activity Relationship; D013997:Time Factors; D014170:Transformation, Genetic / Q000187:drug effects
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000535:Aluminum; D008358:Mannose