title: New tool for spreading proteins to the environment: Cry1Ab toxin immobilized to bioplastics.
abstract: A new tool to provide an environmentally friendly way to deliver active proteins to the environment has been developed, based on the use of polyhydroxyalkanoate (PHA, bioplastic) granules. To illustrate this novel approach, a derived Cry1Ab insect-specific toxin protein was in vivo immobilized into PHA granules through the polypeptide tag BioF. The new toxin, named Fk-Bt1, was shown to be active against Sesamia nonagrioides (Lepidoptera: Noctuidae). The dose-mortality responses of the new toxin granule formulation (PFk-Bt1) and purified Cry1Ab have been compared, demonstrating the effectiveness of PFk-Bt1 and suggesting a common mode of action.
authors.affiliation: Department of Molecular Microbiology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain.|UMR 163, CEA Cadarache, 13108 St. Paul lez Durance, Cedex, France.
authors.forename: Cristina
authors.initials: C
authors.lastname: Moldes
authors.affiliation: Department of Plant Biology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain.
authors.forename: Gema P
authors.initials: GP
authors.lastname: Farinós
authors.affiliation: Department of Molecular Microbiology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain.
authors.forename: Laura I
authors.initials: LI
authors.lastname: de Eugenio
authors.affiliation: Department of Molecular Microbiology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: García
authors.affiliation: Department of Molecular Microbiology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: García
authors.affiliation: Department of Plant Biology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain.
authors.forename: Félix
authors.initials: F
authors.lastname: Ortego
authors.affiliation: Department of Plant Biology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Hernández-Crespo
authors.affiliation: Department of Plant Biology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Castañera
authors.affiliation: Department of Molecular Microbiology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain. auxi@cib.csic.es.
authors.forename: María A
authors.initials: MA
authors.lastname: Prieto
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000737:chemistry / Q000494:pharmacology*; D003594:Cytoplasmic Granules / Q000737:chemistry; D004731:Endotoxins / Q000737:chemistry / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000737:chemistry / Q000494:pharmacology*; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D007915:Lepidoptera / Q000187:drug effects; D010572:Pest Control, Biological / Q000379:methods*; D011108:Polymers; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; D011108:Polymers; D011993:Recombinant Fusion Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis