Coproantigens in taeniasis and echinococcosis.
The application of modern immunodiagnostic or molecular diagnostic techniques has improved the diagnosis of the taeniid cestode infections, echinococcosis and taeniasis. One particularly promising approach is the detection of parasite-specific antigens in faeces (coproantigens). This approach has been applied to both Echinoccocus and Taenia species and it has gained increasingly widespread use. Taeniid coproantigen tests are based on either monoclonal or polyclonal antibodies raised against adult tapeworm antigens. These tests have the following common characteristics; they are largely genus-specific, specificity is high (>95%), parasite antigen can be detected in faeces weeks prior to patency, levels of coproantigen are independent of egg output, coproantigen is stable for days at a range of temperatures (-80 degrees C to 35 degrees C), for several months in formalin-fixed faecal samples, and coproantigen levels drop rapidly (1-5 days) following successful treatment. In the genus Taenia, most work has been done on Taenia solium and coproantigen tests have reliably detected many more tapeworm carriers than microscopy. For Echinococcus species, there is a broad positive correlation between test sensitivity and worm burden with a reliable threshold level for the test of >50 worms. Characterisation of taeniid coproantigens in order to further improve the tests is ongoing. Studies indicate taeniid coproantigens to include high molecular weight (>150 kDa), heavily glycosylated molecules with carbohydrate moieties contributing substantially to the levels of antigen detected in faeces. Application of the existing coproantigen tests in epidemiological and control programmes for Echinococcus and Taenia species infection has begun to contribute to an improved understanding of transmission and of surveillance of these important zoonotic cestodes.
Allan
James C
JC
Cestode Zoonoses Research Group, Bioscience Research Institute University of Salford, UK. James.Allan@Pfizer.com
Craig
Philip S
PS
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D004283:Dog Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology; D004285:Dogs; D004443:Echinococcosis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D004446:Echinococcus / Q000276:immunology; D005243:Feces / Q000469:parasitology*; D006801:Humans; D013621:Taenia / Q000276:immunology; D013622:Taeniasis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology
D000947:Antigens, Helminth