Isolation of novel bacteria able to degrade alpha-halocarboxylic acids by enrichment from environmental samples.
In order to isolate novel bacteria able to degrade alpha-halocarboxylic acids a variety of culturing strategies were implemented. Eight pure cultures were obtained and were found to be associated with the Gram negative Proteobacteria and the Gram positive Bacillus and Enterococcus genera. Furthermore, several strains were obtained which were able to degrade the DL-halocarboxylic acids anaerobically. Molecular analysis of the pure cultures led us to conclude that they may possess novel enzymes involved in the biodegradation of the alpha-halocarboxylic acids. These results are the first for nearly 40 years to describe the isolation of Gram positive isolates on an alpha-halocarboxylic acid as the sole source of carbon and energy, which also show the ability to de-toxify the test substrate by releasing chloride.
Kerr
Linda M
LM
Department of Microbiology, University College Cork, College Road, Cork, Ireland.
Marchesi
Julian R
JR
D001407:Bacillus / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002264:Carboxylic Acids / Q000302:isolation & purification / Q000378:metabolism*; D016983:Enterococcus / Q000302:isolation & purification / Q000378:metabolism; D004783:Environmental Microbiology; D019015:Geologic Sediments / Q000382:microbiology*; D006219:Halogens / Q000302:isolation & purification / Q000378:metabolism*; D006846:Hydrocarbons, Halogenated / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny
D002264:Carboxylic Acids; D006219:Halogens; D006846:Hydrocarbons, Halogenated