title: Completion of the genome sequence of Lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus.
abstract: We completed the genome sequence of Lettuce necrotic yellows virus (LNYV) by determining the nucleotide sequences of the 4a (putative phosphoprotein), 4b, M (matrix protein), G (glycoprotein) and L (polymerase) genes. The genome consists of 12,807 nucleotides and encodes six genes in the order 3' leader-N-4a(P)-4b-M-G-L-5' trailer. Sequences were derived from clones of a cDNA library from LNYV genomic RNA and from fragments amplified using reverse transcription-polymerase chain reaction. The 4a protein has a low isoelectric point characteristic for rhabdovirus phosphoproteins. The 4b protein has significant sequence similarities with the movement proteins of capillo- and trichoviruses and may be involved in cell-to-cell movement. The putative G protein sequence contains a predicted 25 amino acids signal peptide and endopeptidase cleavage site, three predicted glycosylation sites and a putative transmembrane domain. The deduced L protein sequence shows similarities with the L proteins of other plant rhabdoviruses and contains polymerase module motifs characteristic for RNA-dependent RNA polymerases of negative-strand RNA viruses. Phylogenetic analysis of this motif among rhabdoviruses placed LNYV in a group with other sequenced cytorhabdoviruses, most closely related to Strawberry crinkle virus.
authors.affiliation: Department of Primary Industries and Fisheries, Queensland Agricultural Biotechnology Centre, Queensland Bioscience Precinct, The University of Queensland, 306 Carmody Rd, St. Lucia, Qld. 4072, Australia. ralf.dietzgen@dpi.qld.gov.au
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Dietzgen
authors.forename: Ben
authors.initials: B
authors.lastname: Callaghan
authors.forename: Thierry
authors.initials: T
authors.lastname: Wetzel
authors.forename: James L
authors.initials: JL
authors.lastname: Dale
mesh_terms: D020816:Amino Acid Motifs; D001483:Base Sequence; D023061:Gene Order; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics; D010802:Phylogeny; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D012209:Rhabdoviridae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014763:Viral Matrix Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D006023:Glycoproteins; D010750:Phosphoproteins; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase