[Comparison of the cytotoxicity in vitro among six types of nano-silver base inorganic antibacterial agents].
OBJECTIVE
To evaluate the biocompatibility of nano-silver base inorganic antibacterial agents and compare the cytotoxicity in vitro among six types of nano-silver base inorganic antibacterial agents.


METHODS
FUMAT T200-4, HN300, Novaron, Kangwang, MOD and SR1000 were diluted to different concentrations, such as 100 g/L, 50 g/L, 25 g/L and 12.5 g/L. The cytotoxicity in vitro of these agents on rat's fibroblast was assayed with MTT method. And the grades of cytotoxicity were compared.


RESULTS
High concentrations of nano-silver base inorganic antibacterial agents had cytotoxic effects on rat's fibroblasts L-929. As the concentration decreased, the cytotoxicity decreased. No cytotoxic effects were observed at or below the concentration of 25 g/L. FUMAT T200-4, Kongwang and SR1000, with the carrier of phosphate zirconium, had less cytotoxity than the others.


CONCLUSIONS
Nano-Silver base inorganic antibacterial agents, such as FUMAT T200-4, Kangwang, SR1000, have good biocompatibility. And they have the possibility of clinical application. The safe concentration of these agents is at or below 25 g/L.
Zhang
Fu-qiang
FQ
Department of Prosthodontics, School of Stomatology, Affiliated Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China. fredzc@online.sh.cn
She
Wen-jun
WJ
Fu
Yuan-fei
YF
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000633:toxicity*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D005347:Fibroblasts / Q000187:drug effects*; D049329:Nanostructures / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D018030:Silver Compounds / Q000008:administration & dosage / Q000145:classification / Q000633:toxicity*
D000900:Anti-Bacterial Agents; D018030:Silver Compounds