title: Cloning and analysis of Nkx6.3 during CNS and gastrointestinal development.
abstract: Members of the Nkx family of homeodomain proteins are involved in a variety of developmental processes such as cell fate determination in the CNS and in the pancreas. Here we describe the cloning and developmental expression pattern of Nkx6.3, a new member of the Nkx6 subfamily of homeodomain proteins. Nkx6.3 is expressed in the developing CNS and gastro-intestinal tract. In contrast to Nkx6.1 and Nkx6.2 that are broadly expressed in ventral positions of the developing CNS, Nkx6.3 shows a remarkably selective expression in a subpopulation of differentiating V2 neurons at caudal hindbrain levels. The expression of Nkx6.3 at this level depends on the activity of other Nkx6 proteins. In the gut, Nkx6.3 is expressed in duodenal and glandular stomach endoderm and at the end of gestation Nkx6.3 became restricted to the base of the gastric units in the glandular stomach. The expression of Nkx6.3 overlapped with the expression of Nkx6.2 both in the CNS and in the gut. Transient Nkx6.2 expression was also detected in the developing pancreas. However, analysis of Nkx6.2(-/-) mice did not display any obvious aberrations of pancreatic or stomach development.
authors.affiliation: Umeå Centre for Molecular Medicine, UCMM, Umeå University, S-901 87 Umeå, Sweden.
authors.forename: Tomas
authors.initials: T
authors.lastname: Alanentalo
authors.forename: Fabrice
authors.initials: F
authors.lastname: Chatonnet
authors.forename: Mattias
authors.initials: M
authors.lastname: Karlen
authors.forename: Rima
authors.initials: R
authors.lastname: Sulniute
authors.forename: Johan
authors.initials: J
authors.lastname: Ericson
authors.forename: Elisabet
authors.initials: E
authors.lastname: Andersson
authors.forename: Ulf
authors.initials: U
authors.lastname: Ahlgren
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002490:Central Nervous System / Q000196:embryology* / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D041981:Gastrointestinal Tract / Q000196:embryology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics*
chemical_list: D018398:Homeodomain Proteins; D014157:Transcription Factors; D004247:DNA