title: [Effects of immunoglobulin on the neuronal expression of IL-1beta and IL-1ra and the neuronal death at hippocampus in rats with convulsion induced by pentylenetetrazol].
abstract: OBJECTIVE
  To study the effects of immunoglobulin on the neuronal expression of IL-1beta and IL-1ra and the neuronal death at hippocampus in rats with convulsion induced by pentylenetetrazol.
  
  
  METHODS
  The epilepsy model was established by injecting intraperitoneally pentylenetetrazol (PTZ) into Wistar rats. Forty-five rats were randomly divided into three groups, normal control group, PTZ plus intravenous immunoglobulin (PTZ-IVIG); PTZ plus normal saline (PTZ-NS). Neuronal death was assessed by light microscopy with the hematoxylin-eosin (HE) staining and with in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL). IL-1beta and IL-1ra expressions were examined by histochemistry.
  
  
  RESULTS
  The ratio of IL-1beta/IL-1ra at hippocampal CA(1) region in PTZ-IVIG group (0.5 +/- 0.1) was significantly lower than that in PTZ-NS group (1.9 +/- 0.5, t = 12.9, P < 0.05). Apoptotic cell numbers at the hippocampal CA(1) region were significantly decreased in the PTZ-IVIG group, compared to PTZ-NS group (t = 27.1, P < 0.05). The numbers of positive cells were 16.4 +/- 3.3/1000 microm(2) in the former and 41.7 +/- 3.5/1000 microm(2) in the latter. Necrotic cell numbers at the hippocampal CA(1) region were significantly decreased in the PTZ-IVIG group (19.0 +/- 2.6/1000 microm(2)), compared to PTZ-NS group (42.3 +/- 4.9/1000 microm(2), t = 20.9, P < 0.05).
  
  
  CONCLUSIONS
  Immunoglobulin could inhibit neuronal death induced by convulsion and its possible mechanism might be the regulation of IL-1 system in neurons.
authors.affiliation: Department of Pediatrics, Affiliated Hospital, Yanbian University Medical College, Yanji 133000, China.
authors.forename: Ren-zhe
authors.initials: RZ
authors.lastname: An
authors.forename: Guang-hai
authors.initials: GH
authors.lastname: Yan
authors.forename: Dong-ming
authors.initials: DM
authors.lastname: Piao
authors.forename: Yong-ri
authors.initials: YR
authors.lastname: Yin
authors.forename: Yong-xue
authors.initials: YX
authors.lastname: Chi
mesh_terms: D000818:Animals; D017209:Apoptosis; D006624:Hippocampus / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D016756:Immunoglobulins, Intravenous / Q000494:pharmacology*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects*; D010433:Pentylenetetrazole / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism
chemical_list: D016756:Immunoglobulins, Intravenous; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D010433:Pentylenetetrazole