title: [Studies on chemical constituents in leaf of Isatis indigotica].
abstract: OBJECTIVE
  To study the chemical constituents of the leaf of Isatis indigotica.
  
  
  METHODS
  Chromatography and spectral analysis were respectively used to isolate and identify the constituents.
  
  
  RESULTS
  Three compounds were isolated from the ethanol extracts of theleaf of I. indigotica, and identified as indirubin, tryptanthrin and L-pyroglutamic acid.
  
  
  CONCLUSIONS
  L-pyroglutamic acid was isolated from the genus for the first time, and tryptanthrin was isolated from the leaf of this plant for the first time.
authors.affiliation: College of Pharmacy, Tongji Medical Center of Huazhong University of Science and Technology, Wuhan 430030, China. jinlan5952@hotmail.com
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Ruan
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zou
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Cai
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification; D031219:Isatis / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011761:Pyrrolidonecarboxylic Acid / Q000737:chemistry / Q000302:isolation & purification*; D011799:Quinazolines / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004365:Drugs, Chinese Herbal; D007211:Indoles; D011799:Quinazolines; C046243:tryptanthrine; D011761:Pyrrolidonecarboxylic Acid; C027185:indirubin