Recognition of SCCmec types according to typing pattern determined by multienzyme multiplex PCR-amplified fragment length polymorphism analysis of methicillin-resistant Staphylococcus aureus.
Multienzyme multiplex PCR-amplified fragment length polymorphism (ME-AFLP) typing is a reliable and simple method for typing of bacterial species. In this study we analyzed two well-documented strain collections of Staphylococcus aureus and compared ME-AFLP typing results with results of various other typing methods. The discriminatory power of ME-AFLP was found comparable to pulsed-field gel electrophoresis, and typing results were highly concordant. ME-AFLP typing presents a suitable method for prescreening of large strain collections. Furthermore, the obtained typing patterns were found to cluster according to the staphylococcal cassette chromosome mec types of the strains.
van der Zee
Anneke
A
Laboratory of Medical Microbiology, St. Elisabeth Hospital, PO Box 747, 5000 AS Tilburg, The Netherlands. a.vd.zee@elisabeth.nl
Heck
Max
M
Sterks
Marcel
M
Harpal
Airien
A
Spalburg
Emile
E
Kazobagora
Leonard
L
Wannet
Wim
W
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D016106:Methicillin Resistance; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics
D001426:Bacterial Proteins