ApoA-I/phosphatidylcholine discs remodels fast-migrating HDL into slow-migrating HDL as characterized by capillary isotachophoresis.
OBJECTIVE
Capillary isotachophoresis (cITP) is a technique for characterizing plasma lipoprotein subfractions according to their electrophoretic charges. We used this technique to examine the mechanism by which apoA-I/phosphatidylcholine (POPC) discs increase pre-beta HDL.


RESULTS
The cITP analysis was performed using plasma prestained with a lipophilic dye on a Beckman P/ACE MDQ system. Plasma from a patient with lecithin:cholesterol acyltransferase (LCAT) deficiency who had increased apoE-containing HDL was used to characterize the charge distribution of apoA-I/POPC discs. cITP analysis of apoB- and E-depleted plasma of the patient in the presence of apoA-I/POPC discs indicated two major subfractions of apoA-I/POPC discs with mobilities of triglyceride-rich lipoproteins (fast and slow apoA-I). Incubation of whole plasma from a normolipidemic subject in the presence of apoA-I/POPC discs caused a reduction in cITP fast (f)- and intermediate (i)-migrating HDL, and fast and slow apoA-I, and an increase in slow (s)-migrating HDL. The changes in cITP lipoprotein subfractions were not affected by the inhibition of LCAT activity. ApoA-I/POPC discs increased the fractional esterification rate of cholesterol in apoB-depleted plasma.


CONCLUSIONS
ApoA-I/POPC discs remodeled cITP fHDL and iHDL to sHDL independent of LCAT activity.
Zhang
Bo
B
Departments of Cardiology, Fukuoka University School of Medicine, 7-45-1 Nanakuma Jonan-ku, Fukuoka 814-0180, Japan.
Miura
Shin-ichiro
S
Fan
Ping
P
Kumagai
Koichiro
K
Takeuchi
Kazuma
K
Uehara
Yoshinari
Y
McMahon
Monica
M
Rye
Kerry-Anne
KA
Saku
Keijiro
K
D016632:Apolipoprotein A-I / Q000494:pharmacology*; D019075:Electrophoresis, Capillary / Q000379:methods*; D053442:High-Density Lipoproteins, Pre-beta / Q000097:blood*; D006801:Humans; D007863:Lecithin Cholesterol Acyltransferase Deficiency / Q000188:drug therapy; D008075:Lipoproteins, HDL / Q000187:drug effects*; D008991:Monitoring, Physiologic; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000037:antagonists & inhibitors; D010713:Phosphatidylcholines / Q000494:pharmacology*
D016632:Apolipoprotein A-I; D053442:High-Density Lipoproteins, Pre-beta; D008075:Lipoproteins, HDL; D010713:Phosphatidylcholines; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; C028694:1-palmitoyl-2-oleoylphosphatidylcholine