Sensitivity of detection of rhinoviruses in spiked clinical samples by nucleic acid sequence-based amplification in the presence of an internal control.
The objectives of the study were to determine the sensitivity of Nucleic Acid Sequence-Based Amplification (NASBA) for the detection of rhinovirus (RV) serotype 15 in water and in spiked respiratory specimens in the presence of a newly developed internal control (IC). The sensitivity of NASBA on RNA was 10 molecules per reaction. The sensitivity of RV NASBA on RV-15 in water and in respiratory specimens was equal to that in tissue culture. Addition of 10(4) molecules of rhinovirus internal control (RV IC) did not affect the sensitivity. Viral RNA should be extracted from clinical specimens as rapidly as possible after collection, since loss of detectable RNA occurs after 2 h. NASBA allows a sensitive detection of RV RNA in spiked respiratory specimens in the presence of an internal control.
Loens
Katherine
K
Microbiology/Centre for Molecular Diagnostics, University Hospital Antwerp, Wilrijkstraat 10, B-2650 Edegem, Belgium. katherine.loens@uza.be
Ieven
Margareta
M
Pattyn
Stefaan
S
Sillekens
Peter
P
Goossens
Herman
H
D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010614:Pharynx / Q000821:virology; D010850:Picornaviridae Infections / Q000821:virology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D012229:Rhinovirus / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000821:virology
D020121:5' Untranslated Regions; D012367:RNA, Viral