Capillary electrophoresis with chemiluminescence detection of rutin and chlorogenic acid based on its enhancing effect for the luminol-ferricyanide system.
A capillary electrophoresis with chemiluminescence method has been developed for the determination of rutin and chlorogenic acid based on its enhancing effect on the luminol-ferricyanide system. Under the optimum conditions, the analytes could be separated within 5 min, and the detection limits of the proposed method were 0.22 microg/ml for rutin and 0.50 microg/ml for chlorogenic acid, respectively. The method was successfully applied to the analysis of rutin and chlorogenic acid in real samples.
Han
Suqin
S
School of Chemistry and Material Science, Shanxi Normal University, Linfen, P R China.
D002726:Chlorogenic Acid / Q000032:analysis* / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D005292:Ferricyanides / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis* / Q000737:chemistry; D014026:Nicotiana / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D005292:Ferricyanides; C007931:hexacyanoferrate III; D002726:Chlorogenic Acid; D008165:Luminol; D012431:Rutin