title: Interaction of fluorescent molecular rotors with blood plasma proteins.
abstract: Many disease states have associated blood viscosity changes. Molecular rotors, fluorescent molecules with viscosity sensitive quantum yields, have recently been investigated as a new method for biofluid viscosity measurement. Current viscometer measurements are complicated by proteins adhering to surfaces and forming air-surface layers. It is unknown at this time what effects proteins may have on biofluid viscosity measurements using molecular rotors. To answer this question, binding affinities to blood plasma proteins were investigated by equilibrium dialysis for four hydrophilic molecular rotors. Aqueous solutions of 9-[(2-cyano-2-hydroxy-carbonyl)vinyl]julolidine (CCVJ) and three derivatives were prepared and dialyzed against solutions of bovine source albumin, fibrinogen and immunoglobulin G approximating normal physiologic concentrations and fresh-frozen human plasma. After equilibration, dye concentration on each side of the dialysis membrane was assessed by spectrophotometry. The relative binding affinity of the four dyes to the proteins and to the plasma was compared. Affinity of all dyes was highest for albumin. The bound dye fraction showed little change in relation to protein concentration in the physiological concentration range. Diol, the most hydrophilic molecular rotor tested showed the lowest affinity for albumin. This study indicates that hydrophilic molecular rotors are well-suited for biofluid viscosity measurement.
authors.affiliation: Department of Biological Engineering, University of Missouri-Columbia, Columbia, MO 65211, USA.
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Akers
authors.forename: Jay M
authors.initials: JM
authors.lastname: Cupps
authors.forename: Mark A
authors.initials: MA
authors.lastname: Haidekker
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D001798:Blood Proteins / Q000378:metabolism*; D001809:Blood Viscosity; D002417:Cattle; D003956:Dialysis; D005340:Fibrinogen / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000378:metabolism; D010949:Plasma; D012044:Regression Analysis; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D001798:Blood Proteins; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D005340:Fibrinogen