Comparison of octopamine-like immunoreactivity in the brains of the fruit fly and blow fly.
A serum raised against conjugated octopamine reveals structurally comparable systems of perikarya and arborizations in protocerebral neuropils of two species of Diptera, Drosophila melanogaster and Phaenicia sericata; the latter is used extensively for electrophysiological studies of the optic lobes and their central projections. Clusters of cell bodies in the brain as well as midline perikarya provide octopamine-like immunoreactive processes to the optic lobes, circumscribed regions of the protocerebrum and the central complex, particularly the protocerebral bridge, fan-shaped body, and ellipsoid body. Ventral unpaired median somata provide immunoreactive processes within the subesophageal ganglion and tritocerebrum. Ascending neurites from these cells also supply the antennal lobe glomeruli, regions of the lateral protocerebrum, the mushroom body calyces, and the lobula complex. The mushroom body's gamma lobes contain immunoreactive processes that originate from processes that arborize in the protocerebrum. The present observations are discussed with respect to similarities and differences between two species of Diptera, one of which has neurons large enough for intracellular penetrations. The results are also discussed with respect to recent studies on octopamine-immunoreactive organization in honey bees and cockroaches and the suggested roles of octopamine in sensory processing, learning, and memory.
Sinakevitch
Irina
I
Arizona Research Laboratories Division of Neurobiology, University of Arizona, Tucson, Arizona 85719, USA.
Strausfeld
Nicholas J
NJ
D000818:Animals; D004175:Diptera / Q000166:cytology / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000378:metabolism; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000378:metabolism*; D024521:Mushroom Bodies / Q000166:cytology / Q000378:metabolism*; D019581:Neuropil / Q000166:cytology / Q000378:metabolism*; D009655:Octopamine / Q000378:metabolism*
D019476:Insect Proteins; D009655:Octopamine