title: Biodegradable hyaluronic acid/n-carboxyethyl chitosan/protein ternary complexes as implantable carriers for controlled protein release.
abstract: An ampholytic N-carboxyethyl chitosan (CEC), with various isoelectric points (IPs), was synthesized by grafting acrylic acid on chitosan utilizing Michael's reaction. Compared to native chitosan, CEC has enhanced water solubility and dramatically accelerated enzymatic degradation; the rate of degradation is proportional to the degree of substitution (DS). The results from turbidimetric titration and fluorescence studies revealed that CEC formed complexes with either hyaluronic acid (HA) or bovine serum albumin (BSA) within a certain pH range. The HA/CEC/BSA ternary complexes could be prepared by colloid titration with quantitative yield and BSA entrapment. The rate of BSA release from the complexes was affected by pH, ionic strength, DS of CEC, and the molecular weight (MW) of HA. The endurance of BSA release from the complexes could be extended up to 20 d by formulating them with high-MW HA and CEC with low DS.BSA release profiles from HA/CEC-2/BSA complexes.
authors.affiliation: Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Jiang
authors.forename: Yijuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Chaonan
authors.initials: C
authors.lastname: Xu
authors.forename: Kangjie
authors.initials: K
authors.lastname: Zhu
authors.forename: Weiliam
authors.initials: W
authors.lastname: Chen
mesh_terms: D020341:Absorbable Implants; D001671:Biochemistry / Q000379:methods; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D004573:Electrolytes / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009994:Osmolar Concentration; D011506:Proteins / Q000008:administration & dosage / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D004573:Electrolytes; D004798:Enzymes; C495631:N-(2-carboxyethyl)chitosan; D011506:Proteins; D012710:Serum Albumin, Bovine; D006820:Hyaluronic Acid; D048271:Chitosan