title: Molecular characterization of parthenogenic Fasciola sp. in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene.
abstract: Nucleotide sequences of ribosomal internal transcribed spacer (ITS1) and mitochondrial NADH dehydrogenase I (NDI) gene were analyzed to genetically characterize aspermic Fasciola forms in Korea. From the difference in ITS1 sequences, Korean flukes were divided into 3 haplotypes represented by Kor1, Kor2 and Kor1/2, which had nucleotides identical to F. hepatica, F. gigantica and those overlapped between the two species, respectively. NDI sequences also showed that Korean flukes could be classified into 3 distinct haplotypes (Kor1: F. hepatica-type, Kor2a and Kor2b: F. gigantica-type). The sequences of Kor1 and Kor2a were 100% identical to those of the haplotypes Fsp1and Fsp2, respectively, which are major Fasciola forms in Japan. These findings strongly suggest that aspermic Fasciola forms in Korea and Japan originated from same ancestors and have recently spread throughout both countries.
authors.affiliation: Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Itagaki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kikawa
authors.forename: Kunio
authors.initials: K
authors.lastname: Terasaki
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shibahara
authors.forename: Koichi
authors.initials: K
authors.lastname: Fukuda
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005209:Fasciola / Q000145:classification* / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D007723:Korea; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D010312:Parthenogenesis / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D021903:DNA, Ribosomal Spacer; D009245:NADH Dehydrogenase