title: [Effect of electroacupuncture at "Neiguan" (PC 6) on expression of c-fos gene in myocardium of the rat with acute myocardial ischemia].
abstract: OBJECTIVE
  To explore the mechanism of electroacupuncture at "Neiguan" (PC 6) improving acute myocardial ischemia.
  
  
  METHODS
  The rats were randomly divided into a sham operation group, a myocardial ischemia model group and a myocardial ischemia model plus electroacupuncture group. The acute myocardial ischemia model was developed byligation of the descending anterior branch of the coronary artery, and electroacupuncture was given at bilateral "Neiguan" (PC 6). Serum myocardial enzymes was determined by biochemical method and the expression of c-fos mRNA in myocardium was detected by using reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The activities of serum myocardial enzymes and the expression of c-fos mRNA in ischemic myocardium were significantly increased as compared with those in the sham operation group (P < 0.05), and after electroacupuncture they were significantly decreased (P < 0.05).
  
  
  CONCLUSIONS
  The mechanism of electroacupuncture at "Neiguan" (PC 6) improving acute myochadial ischemia is possibly related with down-regulation of expression of c-fos mRNA in myocardium.
authors.affiliation: Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xie
authors.forename: Xun-Chang
authors.initials: XC
authors.lastname: Liang
authors.forename: Hong-Jin
authors.initials: HJ
authors.lastname: Wu
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Ru
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D015671:Electroacupuncture; D016762:Genes, fos; D006801:Humans; D017202:Myocardial Ischemia / Q000235:genetics; D009206:Myocardium / Q000378:metabolism; D051381:Rats