title: [Study on using in vitro 3T3 neutral red uptake to test phototoxicity].
abstract: OBJECTIVE
  To establish neutral red uptake test of 3T3 in vitro to detect phototoxicity of chemicals and study the in vitro 3T3 NUR as an alternative method instead of animal skin phototoxicity test.
  
  
  METHODS
  Using incubated Balb/c3T3 cells to establish the optimal conditions of reaction system.
  
  
  RESULTS
  The concentration of neutral red, the incubate time, pH and the density of cells were the factors effecting test results.
  
  
  CONCLUSIONS
  It is suggested that in vitro 3T3 neutral red uptake test can be used as an alternative instead of animals phototoxicity test.
authors.affiliation: Institute for Environment and Product Related Health Safety, China Center for Disease Control and Prevention, Beijing 100021, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zhang
authors.forename: Shao-Xia
authors.initials: SX
authors.lastname: Dong
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells; D003358:Cosmetics / Q000633:toxicity*; D017484:Dermatitis, Phototoxic; D007202:Indicators and Reagents / Q000378:metabolism*; D008730:Methoxsalen / Q000633:toxicity; D051379:Mice; D009499:Neutral Red / Q000378:metabolism*; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D003358:Cosmetics; D007202:Indicators and Reagents; D009499:Neutral Red; D008730:Methoxsalen