title: [High resolution DNA typing for HLA-A, B, Cw genes by polymerase chain reaction-sequence specific oligonucleotide probes method in the Shenyang Han population].
abstract: OBJECTIVE
  To investigate, at the DNA level, the polymorphism of HLA-A, -B, -Cw genes in the Chinese of Han ethnicity in Shenyang.
  
  
  METHODS
  Hybridization with polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) was used to determine HLA-A, -B and -Cw genotypes of 108 unrelated healthy individuals from a Chinese Han population. These Hans were born and living in the Shenyang area.
  
  
  RESULTS
  The numbers of alleles identified were 21 for HLA-A, 43 for HLA-B, and 23 for HLA-Cw. All the allele frequency distributions were consistent with the Hardy-Weinberg equilibrium.
  
  
  CONCLUSIONS
  Using molecular method, the present authors have analyzed the characteristic of HLA I distribution in a group of indigenous Hans in Shenyang and thus have provided more accurate gene data for use in related researches.
authors.affiliation: Department of Dermatology, First Hospital of Dalian Medical University, Dalian, Liaoning, P. R. China.
authors.forename: Shi-xin
authors.initials: SX
authors.lastname: Han
authors.forename: Ying-bin
authors.initials: YB
authors.lastname: Shang
authors.forename: Jiu-hong
authors.initials: JH
authors.lastname: Li
authors.forename: Long
authors.initials: L
authors.lastname: Geng
authors.forename: Ning
authors.initials: N
authors.lastname: Zhai
authors.forename: Kai
authors.initials: K
authors.lastname: Cao
authors.forename: Fang-ji
authors.initials: FJ
authors.lastname: Song
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D002681:China; D004247:DNA / Q000032:analysis / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D055815:Young Adult
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D015345:Oligonucleotide Probes; D004247:DNA