Development of an enumeration method for arsenic methylating bacteria from mixed culture samples.
Bacterial methylation of arsenic converts inorganic arsenic into volatile and non-volatile methylated species. It plays an important role in the arsenic cycle in the environment. Despite the potential environmental significance of AsMB, an assessment of their population size and activity remains unknown. This study has now established a protocol for enumeration of AsMB by means of the anaerobic-culture-tube, most probable number (MPN) method. Direct detection of volatile arsenic species is then done by GC-MS. This method is advantageous as it can simultaneously enumerate AsMB and acetate and formate-utilizing methanogens. The incubation time for this method was determined to be 6 weeks, sufficient time for AsMB growth.
Islam
S M Atiqul
SM
Department of Urban Engineering, The University of Tokyo, 7-3-1 Hongo Bunkyo-Ku, 113-8656 Tokyo, Japan. atiqul@env.t.u-tokyo.ac.jp
Fukushi
Kensuke
K
Yamamoto
Kazuo
K
D000818:Animals; D001151:Arsenic / Q000378:metabolism*; D001152:Arsenicals / Q000032:analysis; D001421:Bacteria, Anaerobic / Q000166:cytology / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000379:methods; D019149:Bioreactors / Q000382:microbiology; D001711:Biotransformation; D002417:Cattle; D019605:Euryarchaeota / Q000166:cytology; D005243:Feces / Q000382:microbiology; D008401:Gas Chromatography-Mass Spectrometry; D008745:Methylation; D012988:Soil Microbiology; D013997:Time Factors
D001152:Arsenicals; D001151:Arsenic