title: Isolation and characterization of four ascorbate peroxidase cDNAs responsive to water deficit in cowpea leaves.
abstract: OBJECTIVE
  Abiotic stresses stimulate formation of active oxygen species in plant tissues. Among antioxidant mechanisms, H2O2 detoxication by ascorbate peroxidases (APX) plays an important role. Several APX isoforms exist in plant cells, and they have rarely been studied separately. The aim of this work was to study changes in cytosolic, peroxisomal, stromatic and thylakoid APX gene expression in response to progressive drought, rapid desiccation and application of exogenous abscisic acid in the leaves of cowpea (Vigna unguiculata) plants.
  
  
  METHODS
  Two cowpea (V. unguiculata) cultivars, 'EPACE-1' which is drought-tolerant and '1183'which is drought-sensitive, were submitted to drought stress by withholding irrigation. Detached leaves were air-dried or treated with exogenous abscisic acid. APX cDNAs were isolated by PCR and cloned in plasmid vectors. Changes in gene expression were studied using reverse-transcription PCR.
  
  
  RESULTS
  Four new V. unguiculata cDNAs encoding putative cytosolic, peroxisomal and chloroplastic (stromatic and thylakoidal) APX were isolated and characterized. In response to the different treatments, higher increases in steady-state transcript levels of the cytoplasmic and peroxisomal APX genes were observed in '1183' compared with 'EPACE-1'. On the other hand, the expression of the chloroplastic APX genes was stimulated earlier in the tolerant cultivar when submitted to progressive drought.
  
  
  CONCLUSIONS
  Water deficit induced differences in transcript accumulation of APX genes between the two cultivars that were related to their respective tolerance to drought. Chloroplastic APX genes responded early to progressive water deficit in the tolerant plant, suggesting a capacity to efficiently detoxify active oxygen species at their production site. The more sensitive '1183' was also able to respond to drought by activating its whole set of APX genes.
authors.affiliation: Laboratoire d'Ecophysiologie Moléculaire, UMR 137 Biosol, Université Paris 12, 61 avenue du Général de Gaulle, 94010 Créteil Cedex, France. lameta@univ-paris12.fr
authors.forename: Agnès
authors.initials: A
authors.lastname: D'Arcy-Lameta
authors.forename: Roselyne
authors.initials: R
authors.lastname: Ferrari-Iliou
authors.forename: Dominique
authors.initials: D
authors.lastname: Contour-Ansel
authors.forename: Anh-Thu
authors.initials: AT
authors.lastname: Pham-Thi
authors.forename: Yasmine
authors.initials: Y
authors.lastname: Zuily-Fodil
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D060387:Ascorbate Peroxidases; D001483:Base Sequence; D018076:DNA, Complementary / Q000302:isolation & purification / Q000378:metabolism; D003681:Dehydration / Q000378:metabolism; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics*; D018515:Plant Leaves / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D000040:Abscisic Acid; D010544:Peroxidases; D060387:Ascorbate Peroxidases