title: In vitro protective effects of salvianolic acid B on primary hepatocytes and hepatic stellate cells.
abstract: The production of reactive oxygen species (ROS) is believed to be involved in liver injury and hepatic fibrosis. Activation of hepatic stellate cells (HSCs) is a key feature of liver fibrosis. Salvia miltiorrhiza is a traditional Chinese herb used in the treatment of cardiovascular and liver diseases to resolve stasis. The effects of salvianolic acid B (Sal B), a major component of Salvia miltiorrhiza, on oxidative damage include free radical DPPH scavenging, malondialdehyde (MDA) formation and ROS generation in primary rat hepatocytes and HSCs, and on alpha-SMA, and collagen expression in transforming growth factor-beta1 (TGF-beta1)-stimulated HSCs were examined. Results indicated that Sal B scavenged DPPH potently with an IC50 2.2+/-0.2 microg/ml (3.06+/-0.3 microM), inhibited lipid peroxidation and eliminated ROS accumulation in a concentration-dependent manner on primary rat hepatocytes and HSCs. Sal B also reduced alpha-SMA and collagen synthesis and deposition in HSCs, and had no direct cytotoxicity on both hepatocytes and HSCs. Our results suggest that Sal B ameliorated oxidative damage and eliminated ROS accumulation in hepatocytes, and attenuated HSC activation, potentially conferring hepatoprotective and anti-fibrogenic effects.
authors.affiliation: National Research Institute of Chinese Medicine, Taipei 112, Taiwan.
authors.forename: Yun-Lian
authors.initials: YL
authors.lastname: Lin
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Wu
authors.forename: Miao-Hua
authors.initials: MH
authors.lastname: Luo
authors.forename: Yeh-Jeng
authors.initials: YJ
authors.lastname: Huang
authors.forename: Chuen-Neu
authors.initials: CN
authors.lastname: Wang
authors.forename: Ming-Shi
authors.initials: MS
authors.lastname: Shiao
authors.forename: Yi-Tsau
authors.initials: YT
authors.lastname: Huang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D002478:Cells, Cultured; D016166:Free Radical Scavengers / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D053630:Depsides
chemical_list: D000199:Actins; D001572:Benzofurans; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D017382:Reactive Oxygen Species; D016212:Transforming Growth Factor beta; C076944:salvianolic acid B; D053630:Depsides