Identification of microsatellite markers linked to progressive retinal atrophy in American Eskimo Dogs.
OBJECTIVE
To identify microsatellite markers linked to progressive retinal atrophy (PRA) in American Eskimo Dogs.


METHODS
Blood samples or buccal epithelial cells from 66 American Eskimo Dogs, including 53 PRA-unaffected and 13 PRA-affected dogs.


METHODS
The genotypes of unaffected and affected dogs were determined by use of microsatellite markers spanning canine chromosome 9 (CFA09). Homozygosity mapping was used to detect linkage between markers and the gene locus for PRA.


RESULTS
Significant allelic association between marker alleles and the gene locus for PRA was detected for GALK1 and TK1, indicating linkage between these markers and the causative gene locus for PRA.


CONCLUSIONS
These data indicate that PRA in American Eskimo Dogs is located on CFA09 and allow for the development of a microsatellite-based test to identify carrier (unaffected) and affected dogs before clinical signs appear.
Moody
Jessica A
JA
Department of Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.
Famula
Thomas R
TR
Sampson
Ruth C
RC
Murphy
Keith E
KE
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D015785:Eye Diseases, Hereditary / Q000235:genetics / Q000662:veterinary*; D005260:Female; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012164:Retinal Diseases / Q000235:genetics / Q000662:veterinary*
D004247:DNA