Pyrosequencing Bacillus anthracis.
Pyrosequencing technology is a sequencing method that screens DNA nucleotide incorporation in real time. A set of coupled enzymatic reactions, together with bioluminescence, detects incorporated nucleotides in the form of light pulses, which produces a profile of characteristic peaks in a pyrogram. We used this technology to identify the warfare agent Bacillus anthracis by sequencing 4 single nucleotide polymorphisms (SNPs) in the rpoB gene as chromosomal markers for B. anthracis. In addition, 1 segment in each of the B. anthracis plasmids pXO1 and pXO2 was analyzed to determine the virulence status of the bacterial strains. Pyrosequencing technology is a powerful method to identify B. anthracis.
Wahab
Tara
T
Swedish Institute for Infectious Disease Control, Solna, Sweden.
Hjalmarsson
Sandra
S
Wollin
Ralfh
R
Engstrand
Lars
L
D000818:Animals; D001407:Bacillus / Q000145:classification / Q000235:genetics; D001408:Bacillus anthracis / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D015373:Bacterial Typing Techniques; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005838:Genotype; D006801:Humans; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity; D014774:Virulence
D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit