title: A clonogenic bone marrow progenitor specific for macrophages and dendritic cells.
abstract: Macrophages and dendritic cells (DCs) are crucial for immune and inflammatory responses and belong to a network of cells that has been termed the mononuclear phagocyte system (MPS). However, the origin and lineage of these cells remain poorly understood. Here, we describe the isolation and clonal analysis of a mouse bone marrow progenitor that is specific for monocytes, several macrophage subsets, and resident spleen DCs in vivo. It was also possible to recapitulate this differentiation in vitro by using treatment with the cytokines macrophage colony-stimulating factor and granulocyte-macrophage colony-stimulating factor. Thus, macrophages and DCs appear to renew from a common progenitor, providing a cellular and molecular basis for the concept of the MPS.
authors.affiliation: INSERM, Laboratory of Mononuclear Phagocyte Biology, Avenir Team, Necker Enfants Malades Institute, 75015 Paris, France.
authors.forename: Darin K
authors.initials: DK
authors.lastname: Fogg
authors.forename: Claire
authors.initials: C
authors.lastname: Sibon
authors.forename: Chaouki
authors.initials: C
authors.lastname: Miled
authors.forename: Steffen
authors.initials: S
authors.lastname: Jung
authors.forename: Pierre
authors.initials: P
authors.lastname: Aucouturier
authors.forename: Dan R
authors.initials: DR
authors.lastname: Littman
authors.forename: Ana
authors.initials: A
authors.lastname: Cumano
authors.forename: Frederic
authors.initials: F
authors.lastname: Geissmann
mesh_terms: D000818:Animals; D000073883:CX3C Chemokine Receptor 1; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D002999:Clone Cells; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D003713:Dendritic Cells / Q000166:cytology*; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D018380:Hematopoietic Stem Cell Transplantation; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D023461:Myeloid Progenitor Cells / Q000166:cytology* / Q000276:immunology; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis; D018121:Receptors, Cytokine / Q000032:analysis; D015728:Receptors, HIV / Q000032:analysis; D011994:Recombinant Proteins; D013154:Spleen / Q000166:cytology
chemical_list: D000073883:CX3C Chemokine Receptor 1; D003115:Colony-Stimulating Factors; D018121:Receptors, Cytokine; D015728:Receptors, HIV; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D019009:Proto-Oncogene Proteins c-kit