title: [Experimental study of cartilage reconstruction using directed inducing MSCs-alginate complex].
abstract: OBJECTIVE
  To evaluate the practicability of cartilage reconstruction using directed inducing bone marrow stem cells(MSCs)- alginate complex.
  
  
  METHODS
  The MSCs were seperated by gradient centrifugation on Percoll, then were induced into chon-drogenic differentiation. After 14 days,immunohistochemical techique was applied to detect the expression of collagen type II. Then, the MSCs and calcium alginate were mixed to form a complex and were planted under the skin of nude mice. Histochemical or immuno-histochemical tests were made at 4 weeks and 8 weeks later.
  
  
  RESULTS
  The MSCs showed the positive result of collagen type II. There were chondrcytes and cartilage lacuna at 4 weeks later, and chondrocytes and the collagen secreted by them became more at 8 weeks later. But, the MSCs without directed inducing did not show the character of the chondrocyte.
  
  
  CONCLUSIONS
  The way of using directed inducing MSCs-alginate complex can form cartilage; it is possible to repair the defect of cartilage in clinical practice.
authors.affiliation: Department of Otolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Wang
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Wang
authors.forename: Chuan-Yu
authors.initials: CY
authors.lastname: Liang
authors.forename: Wen
authors.initials: W
authors.lastname: Li
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D020219:Chondrogenesis; D024043:Collagen Type II / Q000096:biosynthesis; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D051379:Mice; D008819:Mice, Nude; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D024043:Collagen Type II; D006603:Hexuronic Acids; D020723:Glucuronic Acid