Isolation of a novel N-acetyl-D-lactosamine specific lectin from Alocasia cucullata (Schott.).
An N-acetyl-D: -lactosamine (LacNAc) specific lectin from tubers of Alocasia cucullata was purified by affinity chromatography on asialofetuin-linked amino activated silica. The pure lectin showed a single band in SDS-PAGE at pH 8.8 and was a homotetramer with a subunit molecular mass of 13.5 kDa and native molecular mass of 53 kDa. It was heat stable up to 55 degrees C for 15 min and showed optimum hemagglutination activity from pH 2 to 11. The lectin was affected by denaturing agents such as urea (2 M: ), thiourea (2 M: ) and guanidine-HCl (0.5 M: ) and did not require Ca2+ and Mn2+ for its activity. It was a potent mitogen at 10 microg/ml towards human peripheral blood mononuclear cells with 50% growth inhibitory potential towards SiHa (human cervix ) cancer cell line at 100 microg/ml.
Kaur
Amandeep
A
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, 143 005, Amritsar, Punjab, India.
Kamboj
Sukhdev Singh
SS
Singh
Jatinder
J
Saxena
A K
AK
Dhuna
Vikram
V
D031055:Alocasia / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004355:Drug Stability; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008970:Molecular Weight; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology*; D037121:Plant Lectins / Q000008:administration & dosage* / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D011489:Protein Denaturation
D037121:Plant Lectins