title: [Inhibitory effects of esophageal cancer related gene 2 on proliferation of human esophageal cancer cell EC9706].
abstract: OBJECTIVE
  To investigate the inhibitory role of esophageal cancer related gene 2 (ECRG2) on proliferation of human esophageal cancer cell EC9706.
  
  
  METHODS
  Recombinant plasmid pcDNA3.1-ECRG2 with ECRG2 open reading frame was constructed. The cells were transfected with either pcDNA3.1 or pcDNA3.1-ECRG2 using Lipofectamin 2000. The expression of ECRG2 protein was examined by Dot Blot analysis. The effects of ECRG2 on cell proliferation and malignant was analyzed by colony formation assay. The variation of P53 and P21 were detected in EC9706 cells with or without expression of ECRG2.
  
  
  RESULTS
  The plasmid of pcDNA3.1-ECRG2 was successfully established. Colony formation activity of EC9706/pcDNA3.1-ECRG2 was 18% while that of the control cell was 55% in six well plate (P < 0.05). The activity of anchorage-independent proliferation of EC9706/pcDNA3.1-ECRG2 was lower than that of EC9706/pcDNA3.1 in soft agar. After transfected with pcDNA3.1-ECRG2, the expression of P53 and P21 were higher than control.
  
  
  CONCLUSIONS
  ECRG2 can reduced the abilities of proliferation and of anchorage-independent proliferation of EC9706 cells which is through p53 pathway possibility.
authors.affiliation: Department of Etiology & Carcinogenesis, Cancer Institute & Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China.
authors.forename: Mei-ning
authors.initials: MN
authors.lastname: Li
authors.forename: Ge
authors.initials: G
authors.lastname: Huang
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Guo
authors.forename: Shih-hsin
authors.initials: SH
authors.lastname: Lu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D053491:Proteinase Inhibitory Proteins, Secretory; D011994:Recombinant Proteins / Q000235:genetics; D000074162:Serine Peptidase Inhibitors, Kazal Type; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D053491:Proteinase Inhibitory Proteins, Secretory; D011994:Recombinant Proteins; C473799:SPINK7 protein, human; D000074162:Serine Peptidase Inhibitors, Kazal Type; D025521:Tumor Suppressor Proteins