Immunosensor for the detection of Vibrio cholerae O1 using surface plasmon resonance.
An immunosensor for the detection of Vibrio cholerae O1 was developed on the basis of surface plasmon resonance (SPR). A protein G layer was fabricated by means of the chemical coupling between the free amine (-NH2) groups of protein G and the activated carboxyl groups present on a self-assembled monolayer (SAM) consisting of a mixture of 11-mercaptoundecanoic acid (MUA) and hexanethiol (molar ratio of 1:2). A monoclonal antibody, which was confirmed to be specific to V. cholera O1 by the Western blotting technique, was immobilized on the protein G layer. The formation of the SAM, the protein G layer and the sequential binding of the antibody against V. cholera O1 were investigated with SPR spectroscopy. As the number of fabricated layers increased, the minimum angle of plasmon resonance was increased accordingly. The target bacteria, V. cholera O1, was measured with the fabricated immunosensor, whose detection range was between 10(5) and 10(9) cells/mL.
Jyoung
Jy-Young
JY
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Shinsu-Dong, Mapo-Gu, Seoul 121-742, Republic of Korea. jyoung91@hanmir.com
Hong
SaHyun
S
Lee
Woochang
W
Choi
Jeong-Woo
JW
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000276:immunology*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D041241:Vibrio cholerae O1 / Q000276:immunology* / Q000302:isolation & purification*
C029152:G-substrate; D009419:Nerve Tissue Proteins