title: Production of oligoglucuronans by enzymatic depolymerization of nascent glucuronan.
abstract: An original bioreactor process for production of oligoglucuronans was developed using the Sinorhizobium meliloti M5N1CS strain that produces glucuronan. This anionic homopolysaccharide was composed of beta-D-(1,4)-glucopyranosyluronic residues variably O-acetylated at C-3 and/or C-2 positions according to culture conditions. It was depolymerized during its biosynthesis by addition of a fungal glucuronan lyase activity in broths. After purification by tangential ultrafiltration and low-pressure liquid chromatography, (1)H NMR and ESI-Q/TOF-MS characterized the poly- and oligoglucuronic acid fractions. This enzymatic bioreactor strategy authorized the production in gram quantity of an unsaturated and no acetylated oligoglucuronan with a degree of polymerization of 3.
authors.affiliation: Laboratoire des Glucides, EPMV, CNRS FRE2779, IUT/GB, UPJV, Avenue des Facultés, Le Bailly, 80025 Amiens Cedex, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Delattre
authors.forename: Philippe
authors.initials: P
authors.lastname: Michaud
authors.forename: Cyril
authors.initials: C
authors.lastname: Keller
authors.forename: Bernard
authors.initials: B
authors.lastname: Courtois
authors.forename: Josiane
authors.initials: J
authors.lastname: Courtois
mesh_terms: D001709:Biotechnology; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000201:enzymology; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; D011133:Polysaccharide-Lyases; C109209:glucuronan lyase