title: The expression of N-cadherin, fibronectin during chondrogenic differentiation of MSC induced by TGF-beta(1).
abstract: OBJECTIVE
  To investigate the role of N-cadherin and fibronectin during chondrogenesis.
  
  
  METHODS
  Immunohistochemical method and autibody induced changes of aggregation of cells were used to assay the expressions of N-cadherin and fibronectin during cell differentiation.
  
  
  RESULTS
  The N-cadherin was present in the area of the cell nodular area in the 24 hours group after adding chondrogenic revulsant, then there was a down-regulating trend. Fibronectin was expressed in 48 and 72 hours groups after adding chondrogenic revulsant, and showed to be negative afterward. The antibody against fibronectin or N-cadherin could inhibit the formation of cellular nodule markedly.
  
  
  CONCLUSIONS
  Cell adhesion factors play an important role during cell differentiation. TGF-beta(1) stimulates chondrogenesis via transition from an initial N-cadherin-contributing stage to a succedent fibronectin-contributing stage during the process of chondrogenesis in MSCs. Further study is needed to evaluate whether or not it can promote chondrogenesis by transfecting cDNA of CAMs to MSCs.
authors.affiliation: Department of Orthopaedics, Peking University Zhenzhen Hospital, Shenzhen 518036, China.
authors.forename: Wen-Ge
authors.initials: WG
authors.lastname: Li
authors.forename: Xin-Xiang
authors.initials: XX
authors.lastname: Xu