Identification of culturable bacteria present in haemodialysis water and fluid.
Water used to prepare haemodialysis fluid is not sterile, and its microbiological control is important for the prevention of haemodialysis-associated illness. Bacterial populations inhabiting a distribution system for haemodialysis water were studied over an 18-month period. 203 planktonic bacteria isolated on R2A medium were identified by restriction analysis and sequencing of 16S rRNA gene. A diverse bacterial community was detected, containing predominantly Gram-negative members of the Alphaproteobacteria and Betaproteobacteria, as well as representatives of the genus Mycobacterium. Ecological and clinical consequences are discussed: bacteria from the genera Novosphingobium, Pseudomonas and Sphingomonas have been described in the build-up of biofilms, and others like Acinetobacter, Mycobacterium or Brevibacterium may represent a health risk to patients under haemodialysis treatment.
Gomila
Margarita
M
Area Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), Palma de Mallorca, Spain.
Gascó
Joan
J
Busquets
Antonio
A
Gil
José
J
Bernabeu
Rosario
R
Buades
Juan Manuel
JM
Lalucat
Jorge
J
D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D016000:Cluster Analysis; D015169:Colony Count, Microbial; D016172:DNA Fingerprinting; D017931:DNA Primers; D015312:Hemodialysis Solutions / Q000032:analysis*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D017931:DNA Primers; D015312:Hemodialysis Solutions; D012336:RNA, Ribosomal, 16S