Sulfated glycosaminoglycans of the periurethral tissue in women with and without stress urinary incontinence, according to genital prolapse stage.
OBJECTIVE
The objective was to determine sulfated glycosaminoglycans (GAG) of the extracellular matrix (ECM) in women with and without stress urinary incontinence according to genital prolapse stage.


METHODS
Periurethral tissue was obtained from 30 women who underwent surgery for urinary incontinence, for pelvic organ prolapse, or for other benign gynecologic conditions. Biopsy specimens were assessed by biochemical methods to characterize and quantify sulfated glycosaminoglycans. Measurements were made of total glycosaminoglycans, chondroitin sulfate, dermatan sulfate, and of heparan sulfate. Data were compared using the t-test.


RESULTS
In two groups, dermatan sulfate was the most predominant glycosaminoglycan. Women with stress urinary incontinence had significantly more total sulfated glycosaminoglycans (p<0.05) and dermatan sulfate (p<0.05) than women without stress urinary incontinence. We did not observe any differences in chondroitin sulfate and heparan sulfate.


CONCLUSIONS
Women with stress urinary incontinence showed quantitative and qualitative differences in the biochemical characteristics of the extracellular matrix in periurethral tissue by analysis of sulfated glycosaminoglycans, according to genital prolapse stage.
Feldner
Paulo Cezar
PC
Section of Urogynecology and Pelvic Surgery, Department of Gynecology, Federal University of São Paulo, Rua dos Otonis 567, Vila Clementino, São Paulo, SP, Brazil. pfeldner@alfa.epm.br
Kati
Lea Mina
LM
Sartori
Marair Gracio Ferreira
MG
Baracat
Edmund Chada
EC
Rodrigues de Lima
Geraldo
G
Nader
Helena B
HB
Dietrich
Carl P
CP
Girão
Manoel João Batista Castello
MJ
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D003871:Dermatan Sulfate / Q000378:metabolism*; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D012720:Severity of Illness Index; D014521:Urethra / Q000378:metabolism*; D014550:Urinary Incontinence, Stress / Q000378:metabolism* / Q000473:pathology; D014596:Uterine Prolapse / Q000378:metabolism* / Q000473:pathology
D015415:Biomarkers; D006025:Glycosaminoglycans; D003871:Dermatan Sulfate