title: Development of a triplex real-time PCR assay for detection of Panton-Valentine leukocidin toxin genes in clinical isolates of methicillin-resistant Staphylococcus aureus.
abstract: Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA.
authors.affiliation: Provincial Laboratory, Saskatchewan Health, 3211 Albert Street, Regina, Saskatchewan S4S 5W6, Canada. rmcdonal@health.gov.sk.ca
authors.forename: Ryan R
authors.initials: RR
authors.lastname: McDonald
authors.forename: Nick A
authors.initials: NA
authors.lastname: Antonishyn
authors.forename: Toni
authors.initials: T
authors.lastname: Hansen
authors.forename: Laelie A
authors.initials: LA
authors.lastname: Snook
authors.forename: Evelyn
authors.initials: E
authors.lastname: Nagle
authors.forename: Michael R
authors.initials: MR
authors.lastname: Mulvey
authors.forename: Paul N
authors.initials: PN
authors.lastname: Levett
authors.forename: Greg B
authors.initials: GB
authors.lastname: Horsman
mesh_terms: D001427:Bacterial Toxins; D017714:Community-Acquired Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D005098:Exotoxins; D006801:Humans; D007956:Leukocidins; D016106:Methicillin Resistance; D016133:Polymerase Chain Reaction / Q000379:methods*; D018461:Soft Tissue Infections / Q000382:microbiology; D013207:Staphylococcal Skin Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin