title: Enhancement of hepatitis B surface antigen production by adenosine 5'-monophosphate in culture of recombinant Chinese hamster ovary cells.
abstract: Adenosine 5'-monophosphate was found effective in enhancing hepatitis B surface antigen (HBsAg) production in the culture of recombinant Chinese hamster ovary (CHO) cells, but it markedly inhibited cell growth. A culture strategy with periodical medium changes was established to increase HBsAg production and avoid the AMP-induced inhibition of cell growth. The enhanced energy supply might be one of the reasons for the increase in HBsAg production in the presence of AMP.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Luo
authors.forename: Xiangming
authors.initials: X
authors.lastname: Sun
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yi
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000249:Adenosine Monophosphate / Q000494:pharmacology*; D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques; D006224:Cricetinae; D003412:Cricetulus; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*
chemical_list: D006514:Hepatitis B Surface Antigens; D000249:Adenosine Monophosphate