Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology.
The currently accepted culture techniques for the detection of Legionella spp. in water samples (AS/NZS 3896:1998 and ISO 11731 standard methods) are slow and laborious, requiring from 7 to 14 days for a result. We describe a fully validated rapid confirmation technique that uses real-time PCR incorporating the intercalating dye SYTO9 for the direct identification of primary cultures, significantly decreasing turnaround time and allowing faster remedial action to be taken by the industry.
Giglio
Steven
S
Australian Water Quality Centre, PMB 3, Salisbury, South Australia 5108, Australia. steven.giglio@sawater.com.au
Monis
Paul T
PT
Saint
Christopher P
CP
D007841:Latex Fixation Tests / Q000379:methods; D007875:Legionella / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D014871:Water Microbiology