[Determination of genyamicin sulfate in injections by flow-injection electrogenerated chemiluminescence].
A new flow injection electrogenerated chemiluminescence (ECL) method for the determination of genyamicin sulfate was developed. The method is based on the sensitizing effect of genyamicin sulfate on the weak ECL signal of electrochemical oxidation of luminol on the platinum flake electrode in the medium of Na2B4O7. The experimental conditions that influence the ECL intensity were investigated and optimized. Under the optimum conditions, the ECL response is in the range of 1.2 x 10(-9) - 4.0 x 10(-6) g x mL(-1) genyamicin sulfate with a detection limit of 8.0 x 10(-10) g x mL(-1). The relative standard deviation is 2.0%. This method was used to determine genyamicin sulfate in injection samples with satisfactory results.
Ma
Hong-Yan
HY
College of Chemistry and Chemical Engineering, Provincial Key Laboratory of Chemical Reaction Engineering, Yanan University, Yanan 716000, China.
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D017022:Flow Injection Analysis; D005839:Gentamicins / Q000032:analysis* / Q000737:chemistry; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D008165:Luminol / Q000737:chemistry; D010084:Oxidation-Reduction; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D015203:Reproducibility of Results
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D004364:Pharmaceutical Preparations; D010984:Platinum; D008165:Luminol