title: Microbial diversity in production waters of a low-temperature biodegraded oil reservoir.
abstract: Microbial communities in two production waters of a low-temperature and low-salinity petroleum reservoir in Canada were examined using cultural and molecular approaches. The predominant cultivated microorganisms were homoacetogens but sulfate-reducers, acetoclastic methanogens and denitrifiers also gave significant counts. The dominant members of the culturable population were affiliated with the Firmicutes, the "Deltaproteobacteria", the "Epsilonproteobacteria", the Spirochaetes and the Euryarchaeota. 16S rRNA gene clone libraries were also constructed from the total DNA collected from production waters. The bacterial library was entirely composed by a single phylotype related to Arcobacter. The archaeal phylotypes were generally very closely related to members of the orders Methanosarcinales and Methanomicrobiales. Consistent with earlier observations, our data suggest that methanogenesis is a dominant terminal process in the reservoir. Moreover, the cross-evaluation of culture-dependent and -independent techniques also indicates that, contrary to most studies, both acetoclastic and lithotrophic methanogens may be involved in this process. This first investigation of the microbial diversity in a non water-flooded low-temperature and low-salinity petroleum reservoir expands substantially our knowledge of the extent of microbial diversity and highlights the complexity of microbial communities involved in the oil field food chain.
authors.affiliation: Institut Français du Pétrole, 1 et 4, avenue de Bois Préau, 92 852 Rueil-Malmaison Cedex, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Grabowski
authors.forename: Olivier
authors.initials: O
authors.lastname: Nercessian
authors.forename: Françoise
authors.initials: F
authors.lastname: Fayolle
authors.forename: Denis
authors.initials: D
authors.lastname: Blanchet
authors.forename: Christian
authors.initials: C
authors.lastname: Jeanthon
mesh_terms: D000416:Alberta; D001419:Bacteria / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D044822:Biodiversity; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D003080:Cold Temperature; D015169:Colony Count, Microbial; D017931:DNA Primers; D015723:Gene Library; D008969:Molecular Sequence Data; D010578:Petroleum; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D017931:DNA Primers; D010578:Petroleum; D012336:RNA, Ribosomal, 16S