Separation of caffeine and theophylline in poly(dimethylsiloxane) microchannel electrophoresis with electrochemical detection.
A method for the rapid separation and sensitive determination of caffeine and theophylline was presented in poly(dimethylsiloxane) (PDMS) microchannel electrophoresis integrated with electrochemical detection. By using methanol as an additive, the peak shape and resolution were essentially improved. The analytes were well separated within only 40s in the running buffer of 5.0mM borate solution (pH 9.2) containing 10% (v/v) methanol. The linear ranges were from 6microM to 0.6mM and the detection limits were 4microM for caffeine and theophylline, respectively. The proposed method has been successfully applied to determine caffeine and theophylline in rat serum and urine.
Zhang
Qian-Li
QL
The Key Lab of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Hankou Road 22, Nanjing 210093, China.
Lian
Hong-Zhen
HZ
Wang
Wei-Han
WH
Chen
Hong-Yuan
HY
D000818:Animals; D002110:Caffeine / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013806:Theophylline / Q000097:blood / Q000302:isolation & purification* / Q000652:urine
D004129:Dimethylpolysiloxanes; D002110:Caffeine; D013806:Theophylline