[Experimental study of chronic renal tubular-interstitial injury induced by Radix Aristolochiae Fangchi Extract in rats].
OBJECTIVE
Following the former report, we continue to observe the chronic renal tubular-interstitial injury induced by Radix Aristolochiae Fangchi Extract(RAFE) in rats in order to understand whether RAFE in different doses causes the renal tubular-interstitial injury or not.


METHODS
RAFE at the dose of 25.0 mg x kg(-1) x d(-1), 120.0 mg kg(-1) x d(-1) and 200.0 mg x kg(-1) x d(-1) and aristolochic acid (AA, 10.0 mg x kg(-1) d(-1)) was interruptedly administrated by gastric tube for 22 w and 4 w durg withdrawal. Blood, urine and kidney were taken out respectively in 17 w, 22 w and 26 w to measure the indexes of renal function. The morphology of kidney was observed, and Masson staining of kidney were made respectively to compare RAFE groups with AA group.


RESULTS
Pathological changes of renal tissue forms were as follows: All RAFE groups and AA group could develop the pathological process of renal tubular injury-chronic renal interstitial fibrosis. The pathologic changes of RAFE were similar with AA.


CONCLUSIONS
RAFE at all doses administrated interruptedly by gastric tube above 13 w caused chronic renal tubulo-interstitium fibrosis. The renal injury in functions and tissue forms in rats were similar with AA closely. The results showed that AA was the main toxic composition of RAFE.
Du
Gui-You
GY
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China. duguiyou1942@vip.sina.com
Zhou
Su-Juan
SJ
Zhao
Yong
Y
Cu
Hai-Feng
HF
Wang
Xiu-Rong
XR
Li
Li
L
Xiao
Yong-Qing
YQ
Cao
Chun-Yun
CY
Wu
Zi-Lun
ZL
Gao
Shuang-Rong
SR
He
Rong
R
Hui
Lian-Qiang
LQ
Liu
Bao-Yan
BY
D000818:Animals; D030022:Aristolochia / Q000737:chemistry / Q000633:toxicity*; D034341:Aristolochic Acids / Q000302:isolation & purification / Q000633:toxicity; D001806:Blood Urea Nitrogen; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity*; D005260:Female; D005355:Fibrosis / Q000097:blood / Q000139:chemically induced / Q000473:pathology; D007684:Kidney Tubules / Q000473:pathology*; D008297:Male; D009395:Nephritis, Interstitial / Q000097:blood / Q000139:chemically induced* / Q000473:pathology; D018517:Plant Roots / Q000737:chemistry / Q000633:toxicity; D010946:Plants, Medicinal / Q000737:chemistry / Q000633:toxicity*; D011507:Proteinuria / Q000139:chemically induced; D051381:Rats; D017207:Rats, Sprague-Dawley
D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal