Interaction of DNA/polycation complexes with phospholipids: stabilizing strategy for gene delivery.
The interaction between negatively charged lipid vesicles and positively charged DNA/polylysine complexes was studied. The interaction does not lead to release of DNA from the initial complexes. The particles formed are easy to prepare, they have slight negative charge, small dimensions and show good stability in physiological NaCl solution. Such properties might indicate that stabilization of the particles by lipid coating might be a potent strategy, alternative to PEGylation of DNA/polycation complexes. Interaction of DNA/polycation complex particles with lipid vesicles.
Zintchenko
Arkadi
A
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovskeho nam. 2, 162 06 Praha 6, Czech Republic. arkadi.zintchenko@cup.uni-muenchen.de
Konák
Cestmír
C
D002412:Cations; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry*; D018014:Gene Transfer Techniques; D008051:Lipid Bilayers; D049329:Nanostructures; D010743:Phospholipids / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D012996:Solutions
D002412:Cations; D008051:Lipid Bilayers; D010743:Phospholipids; D012709:Serum Albumin; D012996:Solutions; D011107:Polylysine; D012965:Sodium Chloride; D004247:DNA