Lack of poly(ADP-ribose) polymerase-1 gene product enhances cellular sensitivity to arsenite.
Arsenite (As3+) has long been known to induce cancer and other degenerative diseases. Arsenite exerts its toxicity in part by generating reactive oxygen species. Identification of genetic factors that contribute to arsenic mutagenicity and carcinogenicity is critical for the treatment and prevention of arsenic exposure in human population. As poly(ADP-ribose) polymerase (PARP) is critical for genomic DNA stability, role of PARP-1 was evaluated in arsenic-induced cytotoxic and genotoxic effects. Our study revealed that telomere attrition, probably owing to arsenite-induced oxidative stress, was much more pronounced in PARP-1-/- mouse embryonic fibroblasts (MEF; 40%) compared with PARP-1+/+ MEFs (10-20%). Correlation observed between telomere reduction and apoptotic death in PARP-1 null cells strongly indicates that the telomere attrition might be a trigger for enhanced apoptotic death after arsenite treatment. Elevated DNA damage detected by alkaline comet assay points to an impaired repair ability of arsenite-induced DNA lesions in PARP-1-/- MEFs. Consistent with elevated DNA damage, increased micronuclei induction reflecting gross genomic instability was also observed in arsenite-treated PARP-1-/- MEFs. Microarray analysis has revealed that arsenite treatment altered the expression of about 311 genes majority of which have known functions in cellular responses to stress/external stimulus and cell growth and/or maintenance. Our results suggest an important role for PARP-1 gene product in the maintenance of chromosome-genome stability in response to arsenite-induced DNA damage.
Poonepalli
Anuradha
A
Genome Stability Laboratory, Department of Physiology, National University of Singapore.
Balakrishnan
Lakshmidevi
L
Khaw
Aik Kia
AK
Low
Grace Kah Mun
GK
Jayapal
Manikandan
M
Bhattacharjee
Rabindra N
RN
Akira
Shizuo
S
Balajee
Adayabalam S
AS
Hande
M Prakash
MP
D000818:Animals; D018053:Arsenites / Q000633:toxicity*; D002869:Chromosome Aberrations / Q000139:chemically induced; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000502:physiology / Q000648:ultrastructure; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000172:deficiency* / Q000235:genetics; D016615:Telomere / Q000187:drug effects / Q000378:metabolism
D018053:Arsenites; C499321:Parp1 protein, mouse; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; C015001:arsenite