title: Liquid chromatographic enantiomer resolution of N-fluorenylmethoxycarbonyl alpha-amino acids and their ester derivatives on polysaccharide-derived chiral stationary phases.
abstract: The liquid chromatographic enantiomer separation of N-fluorenylmethoxycarbonyl (FMOC) protected alpha-amino acids and their ethyl ester derivatives was performed on polysaccharide-derived chiral stationary phases, Chiralcel OD, Chiralpak AD, and Chiralpak AS. In general, Chiralcel OD and Chiralpak AD showed good performance for resolution of N-FMOC alpha-amino acids and their ethyl esters, respectively. All investigated N-FMOC alpha-amino acid enantiomers were baseline separated on Chiralcel OD or Chiralpak AD, whereas N-FMOC alpha-amino acid ethyl ester enantiomers were baseline resolved (alpha = 1.15-3.03) on Chiralpak AD, except for two analytes. The L-enantiomers of all examined FMOC alpha-amino acid ethyl ester derivatives are preferentially retained on Chiralpak AD, while the elution orders of the other enantiomer separations are not consistent.
authors.affiliation: College of Pharmacy, Chosun University, Kwangju, South Korea.
authors.forename: Yin Hua
authors.initials: YH
authors.lastname: Li
authors.forename: Wonjae
authors.initials: W
authors.lastname: Lee
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D005449:Fluorenes / Q000032:analysis / Q000302:isolation & purification*; D007536:Isomerism; D011134:Polysaccharides
chemical_list: D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D011134:Polysaccharides