Experimental study on the protective effects of edaravone against ischemic spinal cord injury.
OBJECTIVE
Reactive free radical species are thought to be involved in ischemic spinal cord injury. We investigated the effects of edaravone (Mitsubishi Pharma Co, Tokyo, Japan), a free radical scavenger, on spinal ischemia-reperfusion injury in a rabbit model. We also sought to estimate free radicals in the spinal cord using the microdialysis method.


METHODS
Spinal cord ischemia was induced in New Zealand White rabbits. The animals were then divided into 4 groups. In the first experiment, which was carried out in group A (non-edaravone treated) and group B (edaravone treated), we assessed neurologic function and evaluated spinal cord histopathology. In the second experiment, which was performed in group C (non-edaravone treated) and group D (edaravone treated), we sequentially estimated the level of free radical species in the spinal cord with the microdialysis method.


RESULTS
In the first experiment group B showed better neurologic function than group A. The number of viable neurons in the spinal cord gray matter was also higher in group B than in group A. The second experiment revealed that the level of free radical species was lower in group D at 75, 90, and 150 minutes after the beginning of reperfusion compared with levels seen in group C. The appearance of free radical species in the latter group was found to have a biphasic pattern, with peaks at 75 and 150 minutes after the beginning of reperfusion.


CONCLUSIONS
Edaravone exerted a significant protective effect on the spinal cord against ischemia-reperfusion injury by suppressing the level of free radical species, which was demonstrated with the microdialysis method.
Suzuki
Kazuchika
K
The First Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan. kazuchi@fj9.so-net.ne.jp
Kazui
Teruhisa
T
Terada
Hitoshi
H
Umemura
Kazuo
K
Ikeda
Yasuhiko
Y
Bashar
Abul Hasan Muhammad
AH
Yamashita
Katsushi
K
Washiyama
Naoki
N
Suzuki
Takayasu
T
Ohkura
Kazuhiro
K
Yasuike
Junji
J
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D008297:Male; D011817:Rabbits; D015427:Reperfusion Injury / Q000517:prevention & control*; D020760:Spinal Cord Ischemia / Q000517:prevention & control*
D016166:Free Radical Scavengers; D000077553:Edaravone; D000983:Antipyrine