Visualization of alternative splicing in vivo.
The analysis of sequences required for alternative splicing of mRNA has predominantly been performed using cell culture systems. However, the phenotype of cultured cells is almost invariably different from that of cells in the intact animal. It is therefore possible that there are significant differences in the regulation of specific splicing reactions in vivo compared to in cell culture. Here, we describe methods for the visualization and analysis of alternative splicing in vivo using transgenic mice. These methods allow for the analysis of the temporal and tissue-specific regulation of alternative splicing both visually and quantitatively.
Kemp
Paul R
PR
Department of Biochemistry, University of Cambridge, 80, Tennis Court Road, Cambridge, CB2 1GA, UK.
Ellis
Peter D
PD
Smith
Christopher W J
CW
D017398:Alternative Splicing; D000818:Animals; D005822:Genetic Vectors; D005838:Genotype; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D011995:Recombination, Genetic
D012333:RNA, Messenger