title: Mitoxantrone-loaded BSA nanospheres and chitosan nanospheres for local injection against breast cancer and its lymph node metastases. I: Formulation and in vitro characterization.
abstract: Positively charged mitoxantrone (MTO) was absorbed by negatively charged blank bovine serum albumin (BSA) and chitosan (CS) nanospheres to form MTO-BSA-NS and MTO-CS-NS, respectively. In addition to other conditions, values of pH of every step were optimized. On optimized conditions, MTO-BSA-NS of a mean size of 77 nm with an encapsulation yield (EY) of (98.86+/-1.43)% [drug loading rate (DL) (19.82+/-0.29)%] and MTO-CS-NS of a mean size of 75 nm with an EY of (97.57+/-1.00)% [DL (9.78+/-0.10)%] were obtained. After lyophilization and sterilization by (60)Co, the mean size increased about 10% but no significant change was observed in EY and DL. Tests for in vitro release in physiological saline or physiological saline containing 0.5% (w/v) ascorbic acid by a dialysis bag showed sustained release and little burst effect.
authors.affiliation: West China School of Pharmacy, Sichuan University, 610041, China. lubin@wcums.edu.cn
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
authors.forename: Su-Bin
authors.initials: SB
authors.lastname: Xiong
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Yin
authors.forename: Ruo-Bing
authors.initials: RB
authors.lastname: Zhao
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry* / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000188:drug therapy; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000737:chemistry; D004355:Drug Stability; D005612:Freeze Drying; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007267:Injections; D008207:Lymphatic Metastasis / Q000517:prevention & control; D008942:Mitoxantrone / Q000008:administration & dosage / Q000737:chemistry* / Q000627:therapeutic use; D043942:Nanotubes; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012995:Solubility; D013242:Sterilization; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D012710:Serum Albumin, Bovine; D048271:Chitosan; D008942:Mitoxantrone