title: Nickel and cobalt resistance engineered in Escherichia coli by overexpression of serine acetyltransferase from the nickel hyperaccumulator plant Thlaspi goesingense.
abstract: The overexpression of serine acetyltransferase from the Ni-hyperaccumulating plant Thlaspi goesingense causes enhanced nickel and cobalt resistance in Escherichia coli. Furthermore, overexpression of T. goesingense serine acetyltransferase results in enhanced sensitivity to cadmium and has no significant effect on resistance to zinc. Enhanced nickel resistance is directly related to the constitutive overactivation of sulfur assimilation and glutathione biosynthesis, driven by the overproduction of O-acetyl-L-serine, the product of serine acetyltransferase and a positive regulator of the cysteine regulon. Nickel in the serine acetyltransferase-overexpressing strains is not detoxified by coordination or precipitation with sulfur, suggesting that glutathione is involved in reducing the oxidative damage imposed by nickel.
authors.affiliation: Center for Plant Environmental Stress Physiology, Purdue University, 625 Agricultural Mall Drive, West Lafayette, IN 47907, USA.
authors.forename: John L
authors.initials: JL
authors.lastname: Freeman
authors.forename: Michael W
authors.initials: MW
authors.lastname: Persans
authors.forename: Ken
authors.initials: K
authors.lastname: Nieman
authors.forename: David E
authors.initials: DE
authors.lastname: Salt
mesh_terms: D003035:Cobalt / Q000494:pharmacology*; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005818:Genetic Engineering; D005978:Glutathione / Q000378:metabolism; D007700:Kinetics; D009532:Nickel / Q000378:metabolism / Q000494:pharmacology*; D051050:Serine O-Acetyltransferase / Q000235:genetics*; D013455:Sulfur / Q000378:metabolism; D031227:Thlaspi / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D003035:Cobalt; D013455:Sulfur; D009532:Nickel; D051050:Serine O-Acetyltransferase; D005978:Glutathione