Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region.
Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.
Casas
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I
Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, 772 W. 168th Street, Floor 18, New York, NY 10032, USA.
Avellon
A
A
Mosquera
M
M
Jabado
O
O
Echevarria
J E
JE
Campos
R H
RH
Rewers
M
M
Perez-Breña
P
P
Lipkin
W I
WI
Palacios
G
G
D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D036022:Capsid Proteins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D012703:Serotyping
D036022:Capsid Proteins; D004279:DNA, Viral; C018315:hexon capsid protein, Adenovirus