[Estimation of sodium/iodide symporter gene expression (NIS) in thyroid cancer by RT-PCR technique (preliminary study)].
NIS gene is located on chromosome 19 and encodes 643 amino acid protein. It belongs to membrane Na+ dependent glucose symporter proteins family. In normal thyroid is located in basolateral membrane of thyreocyte. It plays a main role in concentrating of iodine in thyreocyte and thus in thyroid hormones synthesis. It was proved that NIS expression influences effectiveness of radioactive iodine therapy in well-differentiated thyroid cancers. The aim of this study was to estimate the NIS expression and its dependence with gender, age and stage in thyroid papillary and follicular cancers. The frozen sections of tissue were used as a source of tumor RNA. RT-PCR technique was employed for NIS expression analysis. We did not find dependence between the presence of NIS expression in investigated thyroid cancers and stage of disease estimated according to TNM classification. We also did not find dependence between NIS expression and gender or sex of the patients. Our results suggest that there is no dependence between NIS expression and iodine uptake.
Tosiek
Milena
M
Molecular Biology Laboratory, Department of Pharmaceutical Biochemistry, Medical University, Muszynskiego 1, 90-151 Lodz, Poland. mmirowski@pharm.am.lodz.pl
Pomorski
Lech
L
Balcerczak
Ewa
E
Mirowski
Marek
M
Czyz
Wojciech
W
D000328:Adult; D018265:Carcinoma, Papillary, Follicular / Q000235:genetics* / Q000473:pathology; D002352:Carrier Proteins / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007455:Iodine / Q000378:metabolism; D008297:Male; D009367:Neoplasm Staging; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027981:Symporters / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D000099209:Sodium-Iodide Symporters
D002352:Carrier Proteins; D007455:Iodine; D027981:Symporters; D000099209:Sodium-Iodide Symporters