Detection of hypothetical proteins in human fetal perireticular nucleus.
There is a legion of hypothetical proteins (HP) in prokaryotic and eukaryotic proteomes and the aim of this study was to describe HP in the perireticular nucleus (PN), a key structure in human brain development. Tissue from four PNs was homogenized and extracted proteins were run on two-dimensional gel electrophoresis followed by in-gel digestion and mass spectrometrical identification of proteins. Several databases were used for obtaining bioinformatic information and searching for functional and structural domains. Five spots represented HP: KIAA0423 protein (Q9Y4F4), hypothetical protein KIAA0153 (Q14166), hypothetical protein DKFZp564A2416 (Q9NTW4), hypothetical protein DKFZp564H1122 (Q9H0W9), and hypothetical protein DKFZp564D1378 (Q9H0R4). These structures were predicted to serve in cell cycle, DNA-condensation, neurogenesis, or apoptosis. The existence of formerly HP proteins in the PN of human fetal brain is shown, thus extending knowledge of the brain proteome and proposing the method used as a suitable analytical tool for searching HP.
Hepner
Felix
F
Department of Pediatrics, Medical University of Vienna, Austria.
Myung
Jae-Kyung
JK
Ulfig
Norbert
N
Pollak
Arnold
A
Lubec
Gert
G
D017209:Apoptosis; D001921:Brain / Q000196:embryology* / Q000378:metabolism; D002453:Cell Cycle; D019295:Computational Biology; D004247:DNA / Q000737:chemistry; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D005313:Fetal Death; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D011247:Pregnancy; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011506:Proteins; D004247:DNA