Proteome analysis of combined effects of androgen and estrogen on the mouse mammary gland.
We and other investigators have previously shown that estrogen and androgen have synergistic effects on the growth of mammary epithelial ducts and alveoli in the Noble mouse. However, the molecular mechanisms behind the synergy are unknown. In the present study, we treated female FVB mice with 17-estrodial (E2) and 5-dihydrotestosterone-bezonate (DHT-B) using slow-releasing hormone pellets for 7 months. Dissection showed that hormone treatment caused atypical hyperplasia of mammary ducts and alveoli. A functional proteomic approach was used to study the holistic protein changes in mammary glands. 2-DE was used to separate proteins. Twenty-five protein spots that were differentially expressed in hormone-treated tissues compared to the control were identified by MALDI-TOF-MS, and ESI-quadrupole-TOF-MS, which include some proteins that are correlative with response to estrogen and androgen stimulation, cells differentiation and growth, signal transduction, metabolism, etc. Real-time RT-PCR was carried out to verify the different expression. These results offered some clues to understand the function of E2 and DHT-B.
Zhang
Lijun
L
College of Life Science, Hunan Normal University, Changsha, P. R. China.
Liu
Xin
X
Zhang
Jian
J
Cao
Rui
R
Lin
Ying
Y
Xie
Jinyun
J
Chen
Ping
P
Sun
Yi
Y
Li
Daiqiang
D
Liang
Songping
S
D000728:Androgens / Q000633:toxicity*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D013196:Dihydrotestosterone / Q000633:toxicity*; D004357:Drug Synergism; D015180:Electrophoresis, Gel, Two-Dimensional; D004848:Epithelium / Q000187:drug effects; D004958:Estradiol / Q000633:toxicity*; D005260:Female; D006965:Hyperplasia / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000728:Androgens; D015415:Biomarkers; D020543:Proteome; D012333:RNA, Messenger; D013196:Dihydrotestosterone; D004958:Estradiol