Cytotoxic clerodane diterpenoids from Casearia membranacea.
Bioassay-guided fractionation of the EtOAc extract of Casearia membranacea leaves and twigs afforded three new clerodane diterpenes, caseamembrins M-O (1-3), and the known rel-(2S,5R,6R,8S,9S,10R,18S,19R)-2-(2-methylbutyryloxy)-6-hydroxy-18,19-di-O-acetyl-18,19-epoxycleroda-3,13(16),14-triene (4) and caseamembrin D (5). The structures of 1-3, including the relative configurations, were established by extensive NMR spectroscopic analyses. The cytotoxic activities of the isolated diterpenoids against human oral epidermoid (KB), medulla (Med), and colon (DLD-1) cancer cell lines were evaluated.
Shen
Ya-Ching
YC
Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan, Republic of China. ycshen@mail.nsysu.edu.tw
Cheng
Yuan-Bin
YB
Ahmed
Atallah F
AF
Lee
Cheng Lin
CL
Chen
Shun-Ying
SY
Chien
Ching-Te
CT
Kuo
Yao-Haur
YH
Tzeng
Guo-Lin
GL
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D037821:Casearia / Q000737:chemistry*; D045785:Diterpenes, Clerodane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013237:Stereoisomerism; D013624:Taiwan; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D045785:Diterpenes, Clerodane; C506493:caseamembrin M; C506494:caseamembrin N; C506495:caseamembrin O