title: [Study on the interaction of tetraiodofluorescein and bovine serum albumin by fluorimetry].
abstract: The binding reaction of tetraiodofluorescein with bovine serum albumin i n aqueous solution was studied by fluorimetry.The binding constant is 2.65 x 10(5) L x mol(-1), the equilibrium constant is 5.7 x 10(4) L x mol(-1), and the binding number is 0.86. The research results of binding model indicate that the hydrophobic force was the main binding force. The presence of BSA can increase the fluorescence intensity of TIF. And the deltaF(F-F0) is linear to the concentration of BSA in a certain range, so the first analytical application was proposed. The linear dynamic range (LDR) was 8 x 10(-7) - 9 x 10(-6) mol x L(-1), the limit of detection (LOD) was 9.88 x 10(-8) mol x L(-1), and the relative standard deviation (RSD) was 3.4%.
authors.affiliation: Department of Chemistry, Shanxi University, Taiyuan 030006, China.
authors.forename: Gai-xian
authors.initials: GX
authors.lastname: Li
authors.forename: Jian-qing
authors.initials: JQ
authors.lastname: Li
authors.forename: Yu-xia
authors.initials: YX
authors.lastname: Wei
authors.forename: Chuan
authors.initials: C
authors.lastname: Dong
mesh_terms: D000465:Algorithms; D000818:Animals; D002417:Cattle; D005452:Fluoresceins / Q000737:chemistry* / Q000378:metabolism; D005453:Fluorescence; D005470:Fluorometry; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008956:Models, Chemical; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005452:Fluoresceins; D012710:Serum Albumin, Bovine