Dual roles for Mcm10 in DNA replication initiation and silencing at the mating-type loci.
Recent studies linking DNA replication proteins to transcriptional silencing suggest that some of the same mechanisms that facilitate the initiation of replication at origins might be involved in establishing repressed chromatin at silencer elements. Our ongoing studies of several mutants of the replication initiation factor Mcm10 of budding yeast revealed an associated defect in the production of mating type pheromones. This observation prompted us to look more directly at the effect of MCM10 mutations on the expression of a reporter gene in the mating type locus and to assay for physical interactions between Mcm10 and known silencing factors. Our findings, that Mcm10 mutants disrupt mating loci silencing and that Mcm10 interacts with Sir2 and Sir3, suggest that Mcm10 also plays an essential, and separable role in transcriptional silencing.
Douglas
Nancy L
NL
Department of Molecular Biology and Genetics, Cornell University, 327 Biotechnology Building, Ithaca, NY 14853-2703, USA. nld6@cornell.edu
Dozier
Samantha K
SK
Donato
Justin J
JJ
D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone; D004261:DNA Replication / Q000235:genetics*; D049770:Genes, Mating Type, Fungal / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism; D064110:Minichromosome Maintenance Proteins; D009154:Mutation / Q000235:genetics; D010675:Pheromones / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D037941:Silencer Elements, Transcriptional / Q000235:genetics*; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D056565:Sirtuin 2; D037761:Sirtuins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C076365:MCM10 protein, S cerevisiae; D010675:Pheromones; C084122:SIR3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; C087778:SIR2 protein, S cerevisiae; D056565:Sirtuin 2; D037761:Sirtuins; D006655:Histone Deacetylases; D064110:Minichromosome Maintenance Proteins