Establishment and characterization of a new erythroblastic leukemia cell line, EEB: phosphatidylglucoside-mediated erythroid differentiation and apoptosis.
A new erythroblastic leukemia cell line (EEB) was established from a patient with early erythroblastic leukemia. The cells had features of immature erythroblasts, including an agranular basophilic cytoplasm and CD36, CD71, CD175s (sialyl-Tn) and CD235a (glycophorin A) expression without CD41 expression, myeloperoxidase activity and platelet-peroxidase activity. The cells were confirmed to be of the erythroid lineage based on expression of the gamma-globin message. They were induced to differentiate into benzidine-positive cells by hemin and delta-amino levulinic acid (delta-ALA). An analysis of cell membrane lipids showed that EEB cells contain a type of glycerolipid, phosphatidylglucose (PhGlc), but not unbranched type 2 chains, i antigens. GL-7 which is a recombinant Fab fragment of GL-2 and binds to PhGlc, induced production of hemoglobin F (HbF) associated with accumulation of the gamma-globin (gamma-globin) message in EEB cells. The GL-7-mediated erythroid differentiation was associated with apoptosis. These results suggest that direct signaling to PhGlc mediates erythroid differentiation and apoptosis in EEB cells.
Kawano-Yamamoto
Chizuru
C
Division of Hematology, Department of Medicine, Jichi Medical School, Minamikawachi, Tochigi 329-0498, Japan.
Muroi
Kazuo
K
Nagatsuka
Yasuko
Y
Higuchi
Masato
M
Kikuchi
Satoru
S
Nagai
Tadashi
T
Hakomori
Sen-Itiroh
SI
Ozawa
Keiya
K
D017209:Apoptosis / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D020732:Cytogenetic Analysis; D004354:Drug Screening Assays, Antitumor; D041905:Erythroid Cells / Q000166:cytology / Q000187:drug effects*; D005914:Globins / Q000187:drug effects / Q000235:genetics; D020404:Glycerophospholipids / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology*; D016130:Immunophenotyping; D004915:Leukemia, Erythroblastic, Acute / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010766:Phosphorylation / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship; D014443:Tyrosine / Q000187:drug effects / Q000378:metabolism
C522802:GL-7 Fab fragment; D020404:Glycerophospholipids; D007140:Immunoglobulin Fab Fragments; C429823:phosphatidylglucose; D014443:Tyrosine; D005914:Globins