[Effect of polylactic acid on the proliferation and differentiation of osteoblast-like cells].
OBJECTIVE
To assess the effects of polylactic acid (PLA) on the proliferation and differentiation of UMR-106 osteoblast-like cells.


METHODS
The ultrastructures of the surface of untreated and pretreated PLA films were observed under electron microscope. UMR-106 osteoblast-like cells were cultured with PLA film which was untreated or treated by some special agents together, then the cells morphology was observed, and methyl thiazolyl tetrazolium (MTT) assay and alkaline phosphatase (ALP) analysis were performed to detect the Tiny distinction between the untreated film and pretreated film was proliferation and differentiation.


RESULTS
found under electron microscope. The cells of material group were seen to have grown in clusters, compared with control. And the proportion of polygon and star cells was higher in the material group than in the control, The values measured by ALP and MTT in the material group were lower than control, yet the distinction was undulatory in different phase of test. The difference in cells morphology between the pretreatment group and control was observed only in the first three days. The values measured by ALP and MTT in the pretreatment group were almost consistent with those in control, and even slightly higher than control sometimes.


CONCLUSIONS
PLA film does not have permanent inhibitory effect on the proliferation and differentiation of osteoblast-like cells. On the contrary, the treated PLA film may accelerate the proliferation and differentiation of cells to some extent. So, PLA film may have the potential for use as a good tissue-engineering frame in the treatment of bone disorders.
Chen
Mi-Na
MN
Department of Infectious Disease, West China Hospital, Sichuan University, Chengdu 610041, China.
Wei
Da-Peng
DP
Fan
Xin-Jian
XJ
Lei
Lei
L
Yuan
Ming-Long
ML
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019344:Lactic Acid / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology*; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D051381:Rats
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)