title: Effect of fluticasone propionate-salmeterol therapy on seasonal changes in airway responsiveness and exhaled nitric oxide levels in patients with pollen-induced asthma.
abstract: BACKGROUND
  There has been concern that in allergic asthmatic patients there might be an interactive effect on inflammation between regular salmeterol use and exposure to allergens, resulting in increased airway responsiveness.
  
  
  OBJECTIVE
  To determine the effects of salmeterol on allergen-induced changes in airway responsiveness and exhaled nitric oxide (ENO) levels in allergic asthmatic patients concomitantly taking inhaled corticosteroids.
  
  
  METHODS
  Forty-two asthmatic patients sensitized to pollen allergens were randomly allocated to treatment with fluticasone propionate-salmeterol (n=21) or fluticasone propionate alone (n=21). Spirometry, the methacholine provocation concentration causing a 20% decline in forced expiratory volume in 1 second (PC20), the adenosine 5'-monophosphate (AMP) PC20, and ENO levels were measured before and at the height of the pollen season after 6 weeks of treatment.
  
  
  RESULTS
  Changes in the methacholine PC20, the AMP PC20, and ENO levels were not significantly different between treatment groups. No significant changes in the AMP PC20 were observed among the fluticasone propionate-salmeterol and fluticasone propionate groups during natural pollen exposure. However, a significant increase in the methacholine PC20 was observed in the fluticasone propionate-salmeterol group (P = .03) and in the fluticasone propionate group (P = .04); ENO concentrations decreased significantly in both groups during natural allergen exposure (P = .009 and .005).
  
  
  CONCLUSIONS
  In patients with pollen-induced asthma, treatment with either fluticasone propionate or fluticasone propionate-salmeterol is associated with significant reductions in methacholine responsiveness and ENO concentrations, even during natural pollen exposure. Furthermore, at least in patients with mild asthma, natural allergen exposure and the regular use of fluticasone propionate-salmeterol are not associated with a greater increase in ENO levels and airway responsiveness than natural allergen exposure and fluticasone propionate use alone.
authors.affiliation: Sección de Alergologia, Hospital Universitario Dr Peset, Valencia, Spain. prieto_jes@gva.es
authors.forename: Luis
authors.initials: L
authors.lastname: Prieto
authors.forename: Valentina
authors.initials: V
authors.lastname: Gutiérrez
authors.forename: Carmen
authors.initials: C
authors.lastname: Pérez-Francés
authors.forename: Carlos
authors.initials: C
authors.lastname: Badiola
authors.forename: Amparo
authors.initials: A
authors.lastname: Lanuza
authors.forename: Laura
authors.initials: L
authors.lastname: Bruno
authors.forename: Anna
authors.initials: A
authors.lastname: Ferrer
mesh_terms: D000249:Adenosine Monophosphate / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000403:Airway Resistance / Q000187:drug effects*; D000420:Albuterol / Q000031:analogs & derivatives* / Q000627:therapeutic use; D000730:Androstadienes / Q000627:therapeutic use*; D001249:Asthma / Q000188:drug therapy* / Q000378:metabolism; D001985:Bronchial Provocation Tests; D001993:Bronchodilator Agents / Q000627:therapeutic use*; D004338:Drug Combinations; D005260:Female; D000068298:Fluticasone; D000068297:Fluticasone-Salmeterol Drug Combination; D005541:Forced Expiratory Volume; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism*; D012621:Seasons; D013449:Sulfonamides / Q000494:pharmacology; D016896:Treatment Outcome
chemical_list: D000730:Androstadienes; D001993:Bronchodilator Agents; D004338:Drug Combinations; D000068297:Fluticasone-Salmeterol Drug Combination; D013449:Sulfonamides; D009569:Nitric Oxide; D000249:Adenosine Monophosphate; C010748:metachloridine; D000068298:Fluticasone; D000420:Albuterol