title: DNA polymorphism and molecular subtyping of the capsular gene cluster of group B streptococcus.
abstract: Serotyping and other phenotypic methods are often used to characterize the capsular polysaccharide of group B streptococci (GBS). We describe a capsular genotyping method that utilizes PCR of capsular polysaccharide synthesis genes (cps) and restriction enzyme digestion. This method facilitates the detection of DNA polymorphism in cps genes and correlates well with serotyping.
authors.affiliation: Microbial Evolution Laboratory, 165 Food Safety & Toxicology Building, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Shannon D
authors.initials: SD
authors.lastname: Manning
authors.forename: David W
authors.initials: DW
authors.lastname: Lacher
authors.forename: H Dele
authors.initials: HD
authors.lastname: Davies
authors.forename: Betsy
authors.initials: B
authors.lastname: Foxman
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Whittam
mesh_terms: D016667:Bacterial Capsules / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005838:Genotype; D006801:Humans; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012703:Serotyping; D013292:Streptococcus agalactiae / Q000145:classification* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004262:DNA Restriction Enzymes