title: Trypanocidal activity of oleoresin and terpenoids isolated from Pinus oocarpa.
abstract: Fractionation with n-hexane/ethyl acetate (1:1 v/v) by open column chromatography of the oleoresin from Pinus oocarpa Schiede yielded two diterpenes, pimaric acid (1) and dehydroabietic acid (5), the sesquiterpene longifolene (3) and a diterpenic mixture containing pimaric acid (1), isopimaric acid (4) and dehydroabietic acid (5). Subsequently, the isolated compounds, the mixture of 1, 4 and 5, the oleoresin and the dehydroabietic acid methyl ester (2), were tested in vitro against epimastigotes of Trypanosoma cruzi, the causative agent of Chagas disease. The most active compounds were 1, 3 and the oleoresin, being as active as nifurtimox, a drug effective in the treatment of acute infection by American trypanosomiasis and used in this work as positive control.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México. juruli@servidor.unam.mx
authors.forename: Julieta
authors.initials: J
authors.lastname: Rubio
authors.forename: José S
authors.initials: JS
authors.lastname: Calderón
authors.forename: Angélica
authors.initials: A
authors.lastname: Flores
authors.forename: Clementina
authors.initials: C
authors.lastname: Castroa
authors.forename: Carlos L
authors.initials: CL
authors.lastname: Céspedes
mesh_terms: D000818:Animals; D014355:Chagas Disease; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D009547:Nifurtimox / Q000494:pharmacology; D008517:Phytotherapy; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology; D012717:Sesquiterpenes / Q000494:pharmacology; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014344:Trypanocidal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D004224:Diterpenes; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; D014344:Trypanocidal Agents; C018620:oleoresins; C035607:longifolene; C008911:pimaric acid; D009547:Nifurtimox