A role for the CPF 3'-end processing machinery in RNAP II-dependent gene looping.
The prevailing view of the RNA polymerase II (RNAP II) transcription cycle is that RNAP II is recruited to the promoter, transcribes a linear DNA template, then terminates transcription and dissociates from the template. Subsequent rounds of transcription are thought to require de novo recruitment of RNAP II to the promoter. Several recent findings, including physical interaction of 3'-end processing factors with both promoter and terminator regions, challenge this concept. Here we report a physical association of promoter and terminator regions of the yeast BUD3 and SEN1 genes. These interactions are transcription-dependent, require the Ssu72 and Pta1 components of the CPF 3'-end processing complex, and require the phosphatase activity of Ssu72. We propose a model for RNAP II transcription in which promoter and terminator regions are juxtaposed, and that the resulting gene loops facilitate transcription reinitiation by the same molecule of RNAP II in a manner dependent upon Ssu72-mediated CTD dephosphorylation.
Ansari
Athar
A
Division of Nucleic Acids Research, Department of Biochemistry, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, 08854, USA.
Hampsey
Michael
M
D002352:Carrier Proteins / Q000378:metabolism*; D004265:DNA Helicases; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D005800:Genes, Fungal / Q000502:physiology*; D046912:Multiprotein Complexes / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases; D011401:Promoter Regions, Genetic / Q000502:physiology; D020365:RNA Helicases; D012319:RNA Polymerase II / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D013728:Terminator Regions, Genetic / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology; D039221:mRNA Cleavage and Polyadenylation Factors / Q000378:metabolism*
C093319:BUD3 protein, S cerevisiae; D002352:Carrier Proteins; D005656:Fungal Proteins; D046912:Multiprotein Complexes; C076229:PTA1 protein, S cerevisiae; C416934:SSU72 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D039221:mRNA Cleavage and Polyadenylation Factors; D012319:RNA Polymerase II; D010749:Phosphoprotein Phosphatases; C542347:SEN1 protein, S cerevisiae; D004265:DNA Helicases; D020365:RNA Helicases