title: Localization and changes of diamine oxidase during cardiopulmonary bypass in rabbits.
abstract: BACKGROUND
  We previously observed increased serum diamine oxidase activity during clinical cardiopulmonary bypass, indicating small intestinal mucosal ischemia followed by bacterial translocation.
  
  
  METHODS
  In seven female rabbits undergoing cardiopulmonary bypass for 1 h, we analyzed the localization of diamine oxidase immunohistochemically, and measured its activity in serum and abdominal organs before and after cardiopulmonary bypass (CPB).
  
  
  RESULTS
  Preoperatively, diamine oxidase activity and immunoreactivity were high in the small intestine, localized to villus tips. Serum activity increased significantly after CPB, whereas small intestinal diamine oxidase decreased with mucosal injury.
  
  
  CONCLUSIONS
  In this model serum diamine oxidase activity appeared to reflect CPB induced intestinal mucosal injury.
authors.affiliation: Second Department of Surgery, Ehime University School of Medicine, Ehime, Japan. tsunooka@m.ehime-u.ac.jp
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tsunooka
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Maeyama
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Doi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Horiuchi
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Miyauchi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Imagawa
authors.forename: Kanji
authors.initials: K
authors.lastname: Kawachi
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000032:analysis* / Q000378:metabolism*; D000818:Animals; D002315:Cardiopulmonary Bypass / Q000009:adverse effects* / Q000662:veterinary; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000201:enzymology* / Q000473:pathology; D011817:Rabbits; D014018:Tissue Distribution
chemical_list: D006631:Amine Oxidase (Copper-Containing)