title: [Effect of truncated PDGF-alpha receptor on proliferation and expression of c-sis mRNA of vascular smooth muscular cells of pulmonary artery].
abstract: OBJECTIVE
  To investigate the effect of truncated PDGF-alpha receptor on proliferation expression of c-sis mRNA of pulmonary artery vascular smooth muscular cells(VSMC).
  
  
  METHODS
  Tissue mass culture was done to get vascular smooth muscular cells of pulmonary artery. Different concentrations of truncated PDGF-alpha receptor and adenoviral recombined body (Ad5CMV-PalphaRtr, ACP) were added into the cultures. VSMC proliferation curves were obtained using MTT test. The expression of c-sis mRNA was detected by PCR.
  
  
  RESULTS
  ACP at 5 ml/L and 10 ml/L concentrations restrained the proliferation of VSMC apparently with the peak of cell growth being attenuated or eliminated. The curve presented ascending tendency only after 7 days. Affected by 5 ml/L ACP, PDGF-BB exerted no significant accelerating effect on the proliferation of VSMC. The expression of c-sis mRNA was up-regulated under the effect of ACP. Affected by 5 ml/L ACP and PDGF-BB, the expression of c-sis mRNA was down-regulated.
  
  
  CONCLUSIONS
  ACP at 5 ml/L and 10 ml/L concentration powerful inhibitor of cellular proliferation for pulmonary artery VSMC. It can increase c-sis mRNA expression significantly and the increase seems to be ACP dosage dependent.
authors.affiliation: Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Su
authors.forename: Han-Min
authors.initials: HM
authors.lastname: Liu
authors.forename: Ju
authors.initials: J
authors.lastname: Gao
authors.forename: Feng-Yi
authors.initials: FY
authors.lastname: Li
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Dong
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Luo
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000473:pathology; D020574:Proto-Oncogene Proteins c-sis / Q000096:biosynthesis* / Q000235:genetics; D011651:Pulmonary Artery / Q000166:cytology* / Q000378:metabolism; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D020796:Receptor, Platelet-Derived Growth Factor alpha