Effect of alcoholic extract of Ayurvedic herb Tinospora cordifolia on the proliferation and myeloid differentiation of bone marrow precursor cells in a tumor-bearing host.
The present study investigates the effect of in vivo administration of alcoholic extract of Tinospora cordifolia whole plant (ALTC) on the proliferation and myeloid differentiation of bone marrow hematopoietic precursor cells in mice bearing a transplantable T cell lymphoma of spontaneous origin designated as Dalton's lymphoma (DL). BMC obtained from ALTC administered DL-bearing mice showed an enhanced BMC proliferation and colony forming ability in vitro in response to L929 conditioned medium as a source of colony stimulating factor (CSF). The number of granulocyte-macrophages colony (CFU-GM) was predominantly higher in the cultures of BMC obtained from ALTC administered mice as compared to mice injected with PBS alone. An increase in the count of bone marrow derived macrophages (BMDM) from ALTC administered mice was also observed along with an increase in the count of tumor associated macrophages. The BMDM obtained from ALTC administered mice showed an enhanced response to signal of LPS for activation to produce IL-1 and TNF. This study indicates that the T. cordifolia can influence the myeloid differentiation of bone marrow progenitor cells and the recruitment of macrophages in response to tumor growth in situ.
Singh
Sukh Mahendra
SM
School of Biotechnology, Banaras Hindu University, Varanasi-221 005, U.P., India. sukh2001@sify.com
Singh
Nisha
N
Shrivastava
Pratima
P
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D017077:Culture Media, Conditioned; D005260:Female; D007375:Interleukin-1 / Q000032:analysis; D016399:Lymphoma, T-Cell / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000187:drug effects; D008297:Male; D008515:Medicine, Ayurvedic; D051379:Mice; D008807:Mice, Inbred BALB C; D023461:Myeloid Progenitor Cells / Q000166:cytology / Q000187:drug effects; D038042:Myelopoiesis / Q000187:drug effects; D009368:Neoplasm Transplantation; D010936:Plant Extracts / Q000494:pharmacology*; D012977:Sodium Nitrite / Q000032:analysis; D013047:Specific Pathogen-Free Organisms; D031610:Tinospora / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D017077:Culture Media, Conditioned; D007375:Interleukin-1; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D012977:Sodium Nitrite