Frequency of HPA-15a and HPA-15b (Gov a/b) human platelet alloantigens in the Croatian population.
BACKGROUND
Human platelet antigen (HPA) genotyping is important for epidemiological studies because the prevalence of particular HPA allotypes differs among various populations and plays a major role in the occurrence of HPA alloimmunization. In Caucasians, antibodies to HPA-1a are the most important causes of neonatal alloimmune thrombocytopenia (NATP). Recent studies suggest that anti-HPA 15a/15b (Gov b, Gov a) might be the most likely candidate antibodies following anti-HPA-1a in inducing NATP.


METHODS
In the present study, HPA-15 system genotype was determined by PCR-SSP method in 279 unrelated subjects from the Croatian population, yielding an HPA-15a and HPA-15b frequency of 0.53 and 0.47, respectively.


RESULTS
Retrograde testing for the presence of anti-HPA-15 antibodies by use of MAIPA in 39 frozen serum samples from serologically negative cases of clinically suspect NATP produced negative results.


CONCLUSIONS
The clinical role of anti-HPA-15 alloantibodies was unable to be confirmed.
Tomicic
Maja
M
Department of Immunohematology, Croatian Institute of Transfusion Medicine, Zagreb, Croatia. maja.tomicic@hztm.htnet.hr
Bingulac-Popovic
Jasna
J
Drazic
Vesna
V
Hundric-Haspl
Zeljka
Z
D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D017523:Croatia; D005260:Female; D058851:GPI-Linked Proteins; D005787:Gene Frequency; D006801:Humans; D007518:Isoantibodies / Q000276:immunology; D007519:Isoantigens / Q000235:genetics* / Q000276:immunology; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D013921:Thrombocytopenia / Q000276:immunology
D015703:Antigens, CD; D001323:Autoantibodies; C120523:CD109 protein, human; D058851:GPI-Linked Proteins; D007518:Isoantibodies; D007519:Isoantigens; D009363:Neoplasm Proteins