title: Different expression of calgizzarin (S100A11) in normal colonic epithelium, adenoma and colorectal carcinoma.
abstract: The aim of the study was to detect proteomic markers usable to distinguish colorectal carcinoma from colon adenoma for a better understanding of the molecular mechanisms in the process of tumourigenesis. Therefore, we microdissected colon carcinoma tissue, epithelial colon adenoma tissue as well as normal adjacent colon epithelium and determined protein profiles by SELDI-TOF MS. A multitude of significantly different signals was detected. For their identification colon biopsis were lysed and subjected to a two-dimensional gel electrophoresis for separation. Subsequently, we identified nearly 100 proteins by tryptic digestion, peptide fingerprint mapping and database search. Calgizzarin (S100A11; S100C) identified by peptide fingerprint mapping correlated very well with a significantly differentially expressed signal found in prior protein profiling. Using an immunodepletion assay we confirmed the identity of this signal as calgizzarin. To localise calgizzarin in tissues we performed immunohistochemistry. For further confirmation of the identity of calgizzarin we re-analysed IHC-positive as well as IHC-negative tissue sections on ProteinChip arrays. This work demonstrates that biomarkers in colorectal cancer can be detected, identified and assessed by a proteomic approach comprising tissue-microdissection, protein profiling and immunological techniques.
authors.affiliation: Core Unit Chip Application (CUCA), Institute of Human Genetics and Anthropology, 07740 Jena, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Melle
authors.forename: Günther
authors.initials: G
authors.lastname: Ernst
authors.forename: Bettina
authors.initials: B
authors.lastname: Schimmel
authors.forename: Annett
authors.initials: A
authors.lastname: Bleul
authors.forename: Henning
authors.initials: H
authors.lastname: Mothes
authors.forename: Roland
authors.initials: R
authors.lastname: Kaufmann
authors.forename: Utz
authors.initials: U
authors.lastname: Settmacher
authors.forename: Ferdinand
authors.initials: F
authors.lastname: Von Eggeling
mesh_terms: D000236:Adenoma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D002277:Carcinoma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D002471:Cell Transformation, Neoplastic; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D016172:DNA Fingerprinting; D003937:Diagnosis, Differential; D020869:Gene Expression Profiling / Q000379:methods*; D005819:Genetic Markers; D006801:Humans; D007413:Intestinal Mucosa; D040081:Protein Array Analysis; D040901:Proteomics; D009418:S100 Proteins / Q000096:biosynthesis*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005819:Genetic Markers; D009418:S100 Proteins; C072337:S100A11 protein, human