title: Continuous monitoring of WBC (biochemistry) in an adult leukemia patient using advanced FTIR-spectroscopy.
abstract: Fourier transform infrared (FTIR)-spectroscopy has been found useful for monitoring the effectiveness of drugs during chemotherapy in leukemia patients. In the present work, spectral changes that occurred in the white blood cells (WBC) of an adult acute myeloid leukemia (AML) patient and their possible utilization for monitoring biochemistry of WBC were investigated. The phosphate absorbance from nucleic acids and the lipid-protein ratio in the WBC decreased immediately after treatment and then increased to levels of a control group. Similar observations were recorded in child patients with acute lymphoblastic leukemia (ALL) who were used as test cases. These parameters maybe used as possible markers to indicate successful remission and suggest that FTIR-spectroscopy may provide a rapid optical method for continuous monitoring or evaluation of a WBC population.
authors.affiliation: Department of Physics and the Cancer Research Center, Ben Gurion University (BGU), Beer-Sheva 84105, Israel.
authors.forename: Ranjit Kumar
authors.initials: RK
authors.lastname: Sahu
authors.forename: Udi
authors.initials: U
authors.lastname: Zelig
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Huleihel
authors.forename: Nir
authors.initials: N
authors.lastname: Brosh
authors.forename: Marina
authors.initials: M
authors.lastname: Talyshinsky
authors.forename: Miriam
authors.initials: M
authors.lastname: Ben-Harosh
authors.forename: Shaul
authors.initials: S
authors.lastname: Mordechai
authors.forename: Jossi
authors.initials: J
authors.lastname: Kapelushnik
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000503:physiopathology*; D007962:Leukocytes; D008297:Male; D008991:Monitoring, Physiologic / Q000379:methods; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy / Q000503:physiopathology*; D011237:Predictive Value of Tests; D012074:Remission Induction; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods