title: Uncover genetic interactions in Caenorhabditis elegans by RNA interference.
abstract: RNA-mediated interference (RNAi) has emerged recently as one of the most powerful functional genomics tools. RNAi has been particularly effective in the nematode worm C. elegans where RNAi has been used to analyse the loss-of-function phenotypes of almost all predicted genes. In this review, we illustrate how RNAi has been used to analyse gene function in C. elegans as well as pointing to some future directions for using RNAi to examine genetic interactions in a systematic manner.
authors.affiliation: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Cambridge, Hinxton, UK. af4@sanger.ac.uk
authors.forename: Angelo
authors.initials: A
authors.lastname: Fortunato
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Fraser
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth / Q000235:genetics; D016678:Genome; D008297:Male; D008957:Models, Genetic; D009154:Mutation; D010641:Phenotype; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000235:genetics; D018510:RNA, Helminth / Q000235:genetics; D014844:Vulva / Q000473:pathology
chemical_list: D029742:Caenorhabditis elegans Proteins; D012330:RNA, Double-Stranded; D018510:RNA, Helminth