Proteomic analysis using protein chips to detect biomarkers in cervical and amniotic fluid in women with intra-amniotic inflammation.
Intra-amniotic inflammation (IAI) may cause preterm birth with poor neonatal out-come. To identify novel biomarkers for IAI, we analyzed amniotic and cervical fluid samples from 27 patients with signs of threatening preterm birth with or without IAI by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Seventeen proteins were significantly overexpressed in amniotic fluid from IAI cases and more often in women with preterm labor than those with rupture of membranes. Five of these were identified as human neutrophil protein 1-3, calgranulin A and B.
Rüetschi
Ulla
U
Department of Clinical Chemistry and Transfusion Medicine, Perinatal Center, Department of Obstetrics and Gynecology, Institute for the Health of Women and Children, Sahlgrenska University Hospital, Göteborg, Sweden.
Rosén
Asa
A
Karlsson
Gösta
G
Zetterberg
Henrik
H
Rymo
Lars
L
Hagberg
Henrik
H
Jacobsson
Bo
B
D000649:Amniocentesis; D000650:Amnion / Q000378:metabolism; D000653:Amniotic Fluid / Q000737:chemistry / Q000378:metabolism*; D015415:Biomarkers / Q000737:chemistry*; D040501:Calgranulin A / Q000378:metabolism; D040502:Calgranulin B / Q000378:metabolism; D002584:Cervix Uteri / Q000378:metabolism; D005260:Female; D005322:Premature Rupture of Fetal Membranes; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000175:diagnosis*; D013058:Mass Spectrometry; D009504:Neutrophils / Q000378:metabolism; D007752:Obstetric Labor, Premature; D011247:Pregnancy; D011248:Pregnancy Complications; D047928:Premature Birth / Q000175:diagnosis* / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000737:chemistry; D020543:Proteome / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D015415:Biomarkers; D040501:Calgranulin A; D040502:Calgranulin B; D011506:Proteins; D020543:Proteome