title: Experimental infection of enterotoxemic Escherichia coli associated with porcine edema disease and its pathologic characteristics in the intestine.
abstract: Edema disease (ED) has become frequent in Japan, but no effective method for experimental infection has been developed. We report here the use of a capsule that resistant against gastric digestion to induce the ED in piglets. Four 21-day-old piglets were used. Shiga toxin 2e-producing Escherichia coli (STEC) cell pellet was encapsulated and administered orally. Two pigs received 1.0x10(10) CFU for two days, and the others received 3.9x10(8) CFU for three days. The high-dose group caused the typical clinical ED signs (palpebral edema or neurologic impairment). Eosinophil infiltration, swollen lymphoid follicles, and edema were observed in the ileum. The kidney had the thrombus in the glomerulus.
authors.affiliation: Laboratory of Animal Science, Kyoto Prefectural University, Japan.
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nakanishi
authors.forename: Keizo
authors.initials: K
authors.lastname: Nakayama
authors.forename: Noritaka
authors.initials: N
authors.lastname: Matsubara
authors.forename: Kazunari
authors.initials: K
authors.lastname: Ushida
mesh_terms: D000818:Animals; D001824:Body Constitution; D001835:Body Weight; D003967:Diarrhea / Q000209:etiology / Q000473:pathology; D004488:Edema Disease of Swine / Q000150:complications / Q000382:microbiology* / Q000473:pathology*; D004927:Escherichia coli Infections / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D007422:Intestines / Q000382:microbiology / Q000473:pathology*; D007668:Kidney / Q000473:pathology; D022641:Shiga Toxin 2 / Q000008:administration & dosage*; D013552:Swine
chemical_list: D022641:Shiga Toxin 2