The influence of G-CSF addition to antibiotic treatment of experimental sepsis on pulmonary tissue.
OBJECTIVE
To investigate the effects of G-CSF addition to antibiotic treatment of experimental sepsis on pulmonary lung tissue.


METHODS
Fifty Sprague-Dawley rats were used. The first four groups received a bolus intraperitoneal injection of Pseudomonas aeruginosa. The first group (Group SAG) received a combined therapy of imipenem and G-CSF, the second group (Group SA) received only imipenem, the third group (Group SG) received only G-CSF, and no antibiotic or G-CSF was given to the fourth group (Group S). The fifth group (Group C) served as the control. Survival rates, peripheral leukocyte counts (PLC) and absolute neutrophil counts (ANC) were obtained, and lung tissues were examined under light microscopy.


RESULTS
Survival rates at the 120th hour were 100% in groups SAG, SA and C; 20% in group SG; and 0% in group S. PLC and ANC values reached their highest levels at the 36th hour in the SAG group and at the 60th hour in the SA group. Pulmonary architecture was better preserved in the SAG, SA and SG groups--in that order--than in the S group.


CONCLUSIONS
Particularly in the early phase of infection, the administration of G-CSF in combination with antibiotics would be appropriate for maximizing the effect of antibiotics as soon as possible and for minimizing the damage caused by inflammation.
Aydin
Sevim
S
Department of Histology and Embryology, Karadeniz Technical University, Farabi Hospital, Trabzon, Türkïye. aydinsevim@yahoo.com
Caylan
Rahmet
R
Aydin
Kemalettin
K
Yulug
Esin
E
Yenilmez
Engin
E
Koksal
Iftihar
I
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D015378:Imipenem / Q000627:therapeutic use*; D007958:Leukocyte Count; D008171:Lung Diseases / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D009504:Neutrophils / Q000378:metabolism; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D016019:Survival Analysis; D016896:Treatment Outcome
D000900:Anti-Bacterial Agents; D016179:Granulocyte Colony-Stimulating Factor; D015378:Imipenem