title: Biological characterization of bovine herpesvirus 1 recombinants possessing the vaccine glycoprotein E negative phenotype.
abstract: Intramolecular recombination is a frequent event during the replication cycle of bovine herpesvirus 1 (BoHV-1). Recombinant viruses frequently arise and survive in cattle after concomitant nasal infections with two BoHV-1 mutants. The consequences of this process, related to herpesvirus evolution, have to be assessed in the context of large use of live marker vaccines based on glycoprotein E (gE) gene deletion. In natural conditions, double nasal infections by vaccine and wild-type strains are likely to occur. This situation might generate virulent recombinant viruses inducing a serological response indistinguishable from the vaccine one. This question was addressed by generating in vitro BoHV-1 recombinants deleted in the gE gene from seven wild-type BoHV-1 strains and one mutant strain deleted in the genes encoding gC and gE. In vitro growth properties were assessed by virus production, one step growth kinetics and plaque size assay. Heterogeneity in the biological properties was shown among the investigated recombinant viruses. The results demonstrated that some recombinants, in spite of their gE minus phenotype, have biological characteristics close to wild-type BoHV-1.
authors.affiliation: Department of Infectious and Parasitic Diseases, Virology and Immunology, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
authors.forename: Benoît
authors.initials: B
authors.lastname: Muylkens
authors.forename: François
authors.initials: F
authors.lastname: Meurens
authors.forename: Frédéric
authors.initials: F
authors.lastname: Schynts
authors.forename: Katalin
authors.initials: K
authors.lastname: de Fays
authors.forename: Aldo
authors.initials: A
authors.lastname: Pourchet
authors.forename: Julien
authors.initials: J
authors.lastname: Thiry
authors.forename: Alain
authors.initials: A
authors.lastname: Vanderplasschen
authors.forename: Nadine
authors.initials: N
authors.lastname: Antoine
authors.forename: Etienne
authors.initials: E
authors.lastname: Thiry
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D022283:Herpesvirus Vaccines / Q000276:immunology*; D007700:Kinetics; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011995:Recombination, Genetic; D017384:Sequence Deletion; D022581:Vaccines, Marker; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*; D010948:Viral Plaque Assay / Q000662:veterinary; D014764:Viral Proteins; D014774:Virulence
chemical_list: D022283:Herpesvirus Vaccines; D022581:Vaccines, Marker; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins