title: Aneuploidy of chromosome 8 and C-MYC amplification in individuals from northern Brazil with gastric adenocarcinoma.
abstract: BACKGROUND
  Gastric cancer is the third most frequent type of neoplasia. In northern Brazil, the State of Pará has a high incidence of this type of neoplasia. Limited data are available so far on the genetic events involved in this disease.
  
  
  METHODS
  Dual-color fluorescence in situ hybridization (FISH) for the C-MYC gene and chromosome 8 centromere was performed in 11 gastric adenocarcinomas.
  
  
  RESULTS
  All cases showed aneuploidy of chromosome 8 and C-MYC amplification, in both the diffuse and the intestinal histopathological types of Laurén. No correlation was found between polysomy 8 and the histopathological characteristics of the tumors. C-MYC amplification, like homogeneously-stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Translocation of C-MYC was observed only in the diffuse-type.
  
  
  CONCLUSIONS
  Chromosome 8 can be used as a marker in the diagnosis of gastric adenocarcinoma. The C-MYC oncogene requires further studies in order to verify if it is, when amplified, an etiological cause of transformation or a consequence of the proliferation process.
authors.affiliation: Human Cytogenetics and Toxicological Genetics Laboratory, Department of Biology, Center of Biological Sciences, Federal University of Pará, Belém, PA, Brazil.
authors.forename: Danielle Queiroz
authors.initials: DQ
authors.lastname: Calcagno
authors.forename: Mariana Ferreira
authors.initials: MF
authors.lastname: Leal
authors.forename: Sylvia Satomi
authors.initials: SS
authors.lastname: Taken
authors.forename: Paulo Pimentel
authors.initials: PP
authors.lastname: Assumpção
authors.forename: Samia
authors.initials: S
authors.lastname: Demachki
authors.forename: Marília de Arruda Cardoso
authors.initials: Mde A
authors.lastname: Smith
authors.forename: Rommel Rodríguez
authors.initials: RR
authors.lastname: Burbano
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000235:genetics*; D000328:Adult; D000483:Alleles; D000782:Aneuploidy; D001938:Brazil; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D005784:Gene Amplification; D015321:Gene Rearrangement; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000097:blood / Q000235:genetics*; D014178:Translocation, Genetic