[Visualization of chromosome territories in interphase nuclei of ovarian nurse cells in Calliphora erythrocephala Mg. (Diptera: Calliphoridae)].
Analysis of localization of chromosomes 2, 3, and 6 of Calliphora erythrocephala Mg. in ovarian nurse cell nuclei with different chromatin structure has shown that the regions of DNA probe hybridization reduced with increasing chromatin compaction. Hybridization of DNA probes of chromosomes 3 and 6 to secondary reticular nuclei demonstrated that chromosomes retain their territories in the nuclei when the chromatin acquires a reticular structure. These results suggest regular organization of the chromosomal apparatus at all stages of the endomitotic cycle, including the stage of highly polyploid reticular nuclei. FISH of DNA probe of the chromosome 2 telomeric region to secondary reticular nuclei revealed a peripheral distribution of the signal. Zones of more intensive DNA probe hybridization have been distinguished. These zones probably are the regions of accumulation of telomeric and (or) centromeric chromosome regions.
Anan'ina
T V
TV
Vedernikov
A E
AE
Vasserlauf
I E
IE
Karamysheva
T V
TV
Rubtsov
N B
NB
Stegniĭ
V N
VN
D000818:Animals; D002875:Chromosomes / Q000502:physiology; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000502:physiology; D009117:Muscidae / Q000166:cytology* / Q000502:physiology; D010053:Ovary / Q000166:cytology* / Q000502:physiology