title: [Preparation and analysis of monoclonal antibody against EphA4 peptide].
abstract: OBJECTIVE
  To prepare monoclonal antibody (MAb) against carboxy-terminus of E-phA4 and to analyze the immunological characteristics and significance of the antibody.
  
  
  METHODS
  Mice were immunized with a chemically synthesized peptide that had been conjugated to KLH (keyhole limpet hemocyamin). A panel of antibodies specifically against EphA4 were obtained by hybridoma technique. The immunological properties and significance of the MAb were analyzed by immunological and immunochemistry techniques.
  
  
  RESULTS
  A hybridoma cell line secreting anti-EphA4 MAb was established. The MAb reacted specifically with human, chicken and mouse EphA4, but did not cross-react with EphA5 and EphA7. The antibody could be used for immunochemical techniques such as ELISA, immunoprecipitation, Western blot and immunohistochemistry.
  
  
  CONCLUSIONS
  The anti-EphA4 MAb generated by immunizing a synthetic peptide possesses excellent immunological properties. MAb can be applied for immunological and immunohistochemical purpose and will become an important tool in the study of EphA4 and its ligand.
authors.affiliation: Departnent of Hematology, Second Xiangya Hospital, Central South University, Changsha 410011, China. zmyang126@hotmail.com
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Yang
authors.forename: Qi
authors.initials: Q
authors.lastname: Weimin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology; D036122:Receptor, EphA4 / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D036122:Receptor, EphA4