Aedes aegypti phosphohexomutases and uridine diphosphate-hexose pyrophosphorylases: comparison of primary sequences, substrate specificities and temporal transcription.
Phosphohexomutases reversibly catalyse the transfer of the phosphate group of a glycosyl phosphate between the C6 and C1 positions, and uridine diphosphate (UDP)-hexose pyrophosphorylases catalyse the synthesis of UDP-hexose from uridine triphosphate (UTP) and hexose-1-phosphate. Both enzyme families are essential for nucleoside diphosphate hexose biosynthesis and are therefore critical for various physiological functions in the midgut of mosquitoes after a blood meal. We cloned and sequenced three phosphohexomutase and two UDP-hexose pyrophosphorylase cDNAs from Aedes aegypti. The products of the cDNAs were expressed and substrate specificities were examined. Herein we describe Ae. aegypti phosphoglucomutase 1, phosphoglucomutase 2, phosphoacetylglucosamine mutase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase. Transcripts of the genes expressing the enzymes are constitutively present in all life stages and blood-feeding does not seem to influence transcript abundance.
Kato
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Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, Madison, WI 53706,, USA.
Mueller
C R
CR
Wessely
V
V
Lan
Q
Q
Christensen
B M
BM
D000330:Aedes / Q000201:enzymology* / Q000235:genetics; D000818:Animals; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D010733:Phosphoglucomutase / Q000235:genetics / Q000378:metabolism*; D017875:Phosphotransferases (Phosphomutases) / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D013379:Substrate Specificity; D014158:Transcription, Genetic; D014530:Uridine Diphosphate / Q000378:metabolism*
D014530:Uridine Diphosphate; D009713:Nucleotidyltransferases; D017875:Phosphotransferases (Phosphomutases); D010733:Phosphoglucomutase; C018718:phosphoacetylglucosamine mutase; C052799:phosphomannomutase