Genotoxic evaluation of the biocomponents of the cricket, Gryllus bimaculatus, using three mutagenicity tests.
The mutagenic potential of the extracted components of Gryllus bimaculatus, a species of cricket, was evaluated using short-term genotoxicity tests including the Ames, chromosome aberration, and micronuclei tests. In a Salmonella typhimurium assay, G. bimaculatus extract did not produce any mutagenic response in the absence or presence of S9 mix with TA98, TA100, TA1535, and TA1537. Chromosome aberration testing showed that G. bimaculatus had no significant effect on Chinese hamster ovary (CHO) cells. In the mouse micronucleus test, no significant alteration in occurrence of micronucleated polychromatic erythrocytes was observed in ICR male mice intraperitoneally administered with G. bimaculatus extract at doses of 15, 150, or 1500 mg/kg. These results indicate that G. bimaculatus extract exerts no mutagenic effect in these in vitro and in vivo systems.
Ahn
Mi Young
MY
Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA, Suwon, South Korea.
Bae
Hye Jin
HJ
Kim
In Sun
IS
Yoo
Eun Jeong
EJ
Kwack
Seung Jun
SJ
Kim
Hyung Sik
HS
Kim
Dong Hyun
DH
Ryu
Kang Sun
KS
Lee
Hee Sam
HS
Kim
Jin Won
JW
Kim
Iksoo
I
Lee
Byung Mu
BM
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D016466:CHO Cells; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D005202:Asia, Eastern; D006135:Gryllidae / Q000737:chemistry* / Q000378:metabolism; D019476:Insect Proteins / Q000633:toxicity*; D008297:Male; D008518:Medicine, East Asian Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D012485:Salmonella typhi / Q000187:drug effects / Q000235:genetics
D019476:Insect Proteins