title: A haploid-specific transcriptional response to irradiation in Saccharomyces cerevisiae.
abstract: Eukaryotic cells respond to DNA damage by arresting the cell cycle and modulating gene expression to ensure efficient DNA repair. We used global transcriptome analysis to investigate the role of ploidy and mating-type in inducing the response to damage in various Saccharomyces cerevisiae strains. We observed a response to DNA damage specific to haploid strains that seemed to be controlled by chromatin regulatory proteins. Consistent with these microarray data, we found that mating-type factors controlled the chromatin-dependent silencing of a reporter gene. Both these analyses demonstrate the existence of an irradiation-specific response in strains (haploid or diploid) with only one mating-type factor. This response depends on the activities of Hdf1 and Sir2. Overall, our results suggest the existence of a new regulation pathway dependent on mating-type factors, chromatin structure remodeling, Sir2 and Hdf1 and independent of Mec1 kinase.
authors.affiliation: CNRS-UMR 2027, Institut Curie, Bât. 110, Centre Universitaire, F-91405 Orsay, France.
authors.forename: G
authors.initials: G
authors.lastname: Mercier
authors.forename: N
authors.initials: N
authors.lastname: Berthault
authors.forename: N
authors.initials: N
authors.lastname: Touleimat
authors.forename: F
authors.initials: F
authors.lastname: Képès
authors.forename: G
authors.initials: G
authors.lastname: Fourel
authors.forename: E
authors.initials: E
authors.lastname: Gilson
authors.forename: M
authors.initials: M
authors.lastname: Dutreix
mesh_terms: D034961:Antigens, Nuclear / Q000502:physiology; D002843:Chromatin / Q000378:metabolism; D002876:Chromosomes, Bacterial; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000502:physiology; D004171:Diploidy; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D005798:Genes, Bacterial; D006238:Haploidy; D006655:Histone Deacetylases / Q000502:physiology; D000072200:Ku Autoantigen; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000502:physiology; D056565:Sirtuin 2; D037761:Sirtuins / Q000502:physiology; D014158:Transcription, Genetic / Q000528:radiation effects
chemical_list: D034961:Antigens, Nuclear; D002843:Chromatin; D004268:DNA-Binding Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; C087778:SIR2 protein, S cerevisiae; D056565:Sirtuin 2; D037761:Sirtuins; D006655:Histone Deacetylases; D000072200:Ku Autoantigen