Kaempferol inhibits apoptosis in vascular smooth muscle induced by a component of oxidized LDL.
Antioxidants such as flavonoids afford protection against oxysterols-induced toxicity. We have investigated the effect of kaempferol and rutin, active components of red wine, in the apoptosis induced by 7beta-hydroxycholesterol in rat vascular smooth muscle cells. 7beta-Hydroxycholesterol induced apoptosis in vascular smooth muscle which include BcL-x(L) degradation, caspase-3 activation and DNA fragmentation. The apoptosis induced by 7beta-hydroxycholesterol was prevented by pretreatment with kaempferol (10-30 microM), but not with rutin. Interestingly preincubation with the estrogen receptor alpha antagonist ICI 182,780 (1 microM) prior to kaempferol partially reverted the antiapoptotic effect of this flavonoid on caspase-3 activation and DNA fragmentation induced by 7beta-hydroxycholesterol. In conclusion, the flavonoid kaempferol, unlike rutin, diminished the apoptosis induced by a component of oxidized low-density lipproteins (oxLDL). This effect was partially mediated by the estrogen receptor alpha.
Ruiz
Emilio
E
Department of Pharmacology, School of Medicine, Universidad Complutense, 28040 Madrid, Spain.
Padilla
Eugenia
E
Redondo
Santiago
S
Gordillo-Moscoso
Antonio
A
Tejerina
Teresa
T
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001013:Aorta, Thoracic / Q000166:cytology / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D047628:Estrogen Receptor alpha / Q000378:metabolism; D006888:Hydroxycholesterols / Q000633:toxicity*; D044949:Kaempferols / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000633:toxicity*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000494:pharmacology; D051020:bcl-X Protein / Q000378:metabolism
D000975:Antioxidants; C494813:Bcl2l1 protein, rat; D047628:Estrogen Receptor alpha; D006888:Hydroxycholesterols; D044949:Kaempferols; D008077:Lipoproteins, LDL; D051020:bcl-X Protein; C099252:oxidized low density lipoprotein; C011724:cholest-5-en-3 beta,7 alpha-diol; D012431:Rutin; C006552:kaempferol