title: Modeling the highly specific ribotoxin recognition of ribosomes.
abstract: The three-dimensional structures of the alpha-sarcin ribotoxin and its delta(7-22) deletion mutant, both complexed with a 20-mer oligonucleotide mimicking the sarcin/ricin loop (SRL) of the ribosome, have been docked into the structure of the Halobacterium marismortui ribosome by fitting the nucleotide atomic coordinates into those of the ribosomal SRL. This study has revealed that two regions of the ribotoxin, residues 11-16 and 84-85, contact the ribosomal proteins L14 (residues 99-105) and L6 (residues 88-92), respectively. The first of these two ribotoxin regions appears to be crucial for its specific ribosome recognition.
authors.affiliation: Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Flor
authors.initials: F
authors.lastname: García-Mayoral
authors.forename: Lucía
authors.initials: L
authors.lastname: García-Ortega
authors.forename: Elisa
authors.initials: E
authors.lastname: Alvarez-García
authors.forename: Marta
authors.initials: M
authors.lastname: Bruix
authors.forename: José G
authors.initials: JG
authors.lastname: Gavilanes
authors.forename: Alvaro Martínez
authors.initials: AM
authors.lastname: del Pozo
mesh_terms: D000595:Amino Acid Sequence; D003603:Cytotoxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004722:Endoribonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D019614:Haloarcula marismortui / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006860:Hydrogen Bonding; D008958:Models, Molecular; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity
chemical_list: D003603:Cytotoxins; D005656:Fungal Proteins; D009843:Oligoribonucleotides; D012269:Ribosomal Proteins; C041174:ribosomal protein L14; C031249:ribosomal protein L6; C014801:alpha-sarcin; D004722:Endoribonucleases