title: Histological analysis of rat dental pulp tissue capped with propolis.
abstract: The aim of the present study was to assess the response of rat dental pulp to direct pulp capping with propolis. Flavonoid and non-flavonoid materials were purified from an ethanol extract of propolis obtained from South Sulawesi, Indonesia. A Class I cavity was prepared on the occlusal surface of the right maxillary first molar in Sprague Dawley rats. The dental pulp was exposed and then capped with a zinc oxide-based filler as a control (group I), or with propolis flavonoids (group II) or non-flavonoids (group III). The animals were sacrificed at week 1, 2 or 4, biopsy samples were obtained, and these were stained and viewed by light microscopy. The results showed that pulp inflammation occurred in groups I and III as early as week 1. No dentin bridge formation was seen in these groups. In contrast, there was no evident inflammatory response in group II at week 1. Mild and moderate pulp inflammation in this group occurred at 2 and 4 weeks after treatment, respectively. Partial dentinal bridge formation was seen in group II at week 4. Therefore, the present results suggest that direct pulp capping with propolis flavonoids in rats may delay dental pulp inflammation and stimulate reparative dentin.
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Hasanuddin University, Makassar 90245, Indonesia.
authors.forename: Ardo
authors.initials: A
authors.lastname: Sabir
authors.forename: Charles R
authors.initials: CR
authors.lastname: Tabbu
authors.forename: Purwanto
authors.initials: P
authors.lastname: Agustiono
authors.forename: Wihaskoro
authors.initials: W
authors.lastname: Sosroseno
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use*; D003737:Dental Cavity Preparation / Q000145:classification; D003738:Dental Cements / Q000627:therapeutic use; D003782:Dental Pulp / Q000187:drug effects*; D003785:Dental Pulp Capping / Q000379:methods*; D003789:Dental Pulp Exposure / Q000628:therapy; D003809:Dentin, Secondary / Q000473:pathology; D005419:Flavonoids / Q000627:therapeutic use; D008297:Male; D008963:Molar; D011429:Propolis / Q000627:therapeutic use*; D011671:Pulpitis / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015034:Zinc Oxide / Q000627:therapeutic use
chemical_list: D000890:Anti-Infective Agents; D003738:Dental Cements; D005419:Flavonoids; D011429:Propolis; D015034:Zinc Oxide