title: Epstein-Barr virus-infected Akata cells are sensitive to histone deacetylase inhibitor TSA-provoked apoptosis.
abstract: Epstein-Barr virus (EBV) infects more than 90 % of the world's population and has a potential oncogenic nature. A histone deacetylase (HDAC) inhibitor, trichostatin A (TSA), has shown potential ability in cancer chemoprevention and treatment, but its effect on EBV-infected Akata cells has not been examined. This study investigated the effect of TSA on the proliferation and apoptosis of the cells. TSA inhibited cell growth and induced cytotoxicity in the EBV-infected Akata cells. TSA treatment sensitively induced apoptosis in the cell, which was demonstrated by the increased number of positively stained cells in the TUNEL assay, the migration of many cells to the sub-G0/G1 phase in flow cytometric analysis, and the ladder formation of genomic DNA. Western blot analysis showed that caspase-dependent pathways are involved in the TSA-induced apoptosis of EBV-infected Akata cells. Overall, this study shows that EBV-infected B lymphomas are quite sensitive to TSA-provoked apoptosis.
authors.affiliation: Laboratory of Cell Biology in Department of Orthodontics and Institute of Oral Biosciences, Chonbuk National University, Chonju 561-756, Korea.
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Kook
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Son
authors.forename: Seong-Kyu
authors.initials: SK
authors.lastname: Han
authors.forename: Hyung-Soon
authors.initials: HS
authors.lastname: Lee
authors.forename: Beom-Tae
authors.initials: BT
authors.lastname: Kim
authors.forename: Yong-Suk
authors.initials: YS
authors.lastname: Jang
authors.forename: Ki-Choon
authors.initials: KC
authors.lastname: Choi
authors.forename: Keun-Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: So-Soon
authors.initials: SS
authors.lastname: Kim
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Lim
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Jeon
authors.forename: Jong-Ghee
authors.initials: JG
authors.lastname: Kim
authors.forename: Jeong-Chae
authors.initials: JC
authors.lastname: Lee
mesh_terms: D017209:Apoptosis; D053181:Caspase 8 / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D056572:Histone Deacetylase Inhibitors; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D019169:Jurkat Cells; D011419:Propidium / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D011419:Propidium; C012589:trichostatin A; D004247:DNA; D053181:Caspase 8