Simultaneous determination of metformin and glimepride in pharmaceutical dosage form by reverse-phase liquid chromatography.
A simple, rapid, and precise reversed-phase liquid chromatographic method has been developed for the simultaneous determination of metformin in combination with glimepride. Under the developed conditions, good separation of the analytes was achieved in short analysis time. Several parameters affecting the separation of the analytes were studied, including pH and the concentration of SDS. The method is validated and shown to be linear in the range of 25 microg/mL to 150 microg/mL for metformin and 0.1 microg/mL to 0.6 microg/mL for glimepride. The method is applied for the analysis of these analytes in commercially available tablets.
Kolte
Bhaskar Laxmanrao
BL
Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra State, India.
Raut
Bharat Baburao
BB
Deo
Adwait Anant
AA
Bagool
Manoj Anil
MA
Shinde
Devanand Baburao
DB
D002853:Chromatography, Liquid; D008687:Metformin / Q000032:analysis*; D013453:Sulfonylurea Compounds / Q000032:analysis*
D013453:Sulfonylurea Compounds; C057619:glimepiride; D008687:Metformin