[A method of HPRE synthesis via transcription by T7 RNA polymerase in vitro].
OBJECTIVE
To synthesize highly pure HBV post-transcriptional regulatory element (HPRE) via transcription in vitro by T7 RNA polymerase.


METHODS
HPRE gene was amplified by PCR from a template containing HBV complete genomic DNA and cloned into plasmid pGEM-11zf. The cloned DNA sequence was transcribed by T7 RNA polymerase.


RESULTS
The construction of HPRE gene recombinant plasmid and production of HPRE via transcription in vitro was successful.


CONCLUSIONS
In vitro transcription by T7 RNA polymerase can be used to synthesize highly pure HPRE.
Huang
Ying
Y
Key Laboratory of Infectious Diseases, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400016, China.
Guo
Jin-jun
JJ
Zhang
Jun
J
Chen
Wei-xian
WX
Huang
Ai-long
AL
D004259:DNA-Directed DNA Polymerase; D012321:DNA-Directed RNA Polymerases; D006515:Hepatitis B virus / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000502:physiology; D014158:Transcription, Genetic; D014764:Viral Proteins
D016601:RNA-Binding Proteins; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase