Assistance of microbial glycolipid antigen processing by CD1e.
Complexes between CD1 molecules and self or microbial glycolipids represent important immunogenic ligands for specific subsets of T cells. However, the function of one of the CD1 family members, CD1e, has yet to be determined. Here, we show that the mycobacterial antigens hexamannosylated phosphatidyl-myo-inositols (PIM6) stimulate CD1b-restricted T cells only after partial digestion of the oligomannose moiety by lysosomal alpha-mannosidase and that soluble CD1e is required for this processing. Furthermore, recombinant CD1e was able to bind glycolipids and assist in the digestion of PIM6. We propose that, through this form of glycolipid editing, CD1e helps expand the repertoire of glycolipidic T cell antigens to optimize antimicrobial immune responses.
de la Salle
Henri
H
INSERM, U725, Etablissement Francais du Sang-Alsace, F-67065 Strasbourg, France.
Mariotti
Sabrina
S
Angenieux
Catherine
C
Gilleron
Martine
M
Garcia-Alles
Luis-Fernando
LF
Malm
Dag
D
Berg
Thomas
T
Paoletti
Samantha
S
Maître
Blandine
B
Mourey
Lionel
L
Salamero
Jean
J
Cazenave
Jean Pierre
JP
Hanau
Daniel
D
Mori
Lucia
L
Puzo
Germain
G
De Libero
Gennaro
G
D000215:Acylation; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology* / Q000378:metabolism*; D018949:Antigens, CD1 / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000201:enzymology / Q000276:immunology; D006017:Glycolipids / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008213:Lymphocyte Activation; D008958:Models, Molecular; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010716:Phosphatidylinositols / Q000276:immunology* / Q000378:metabolism*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D043323:alpha-Mannosidase / Q000276:immunology
D000942:Antigens, Bacterial; D018949:Antigens, CD1; C419394:CD1b antigen; C417738:CD1e antigen; D006017:Glycolipids; D010716:Phosphatidylinositols; D011994:Recombinant Proteins; C034154:phosphatidylinositol mannoside; D043323:alpha-Mannosidase