Influence of Pb contamination in boreal forest soil on the growth and ligninolytic activity of litter-decomposing fungi.
Lignin mineralization activity of three basidiomycetous litter-decomposing fungi (LDF) was studied with humus layer samples taken from a boreal forest soil. The total Pb concentration in the samples was 32,000 mg kg(-1) and water soluble Pb 67 mg kg(-1). Synthetic lignin mineralization by Collybia dryophila and Clitocybe (Lepista) nebularis was strongly inhibited, whereas Stropharia coronilla was more tolerant to Pb stress in soil and liquid cultures. Purified laccases maintained their activity and purified MnPs remained partly active up to a concentration of 1450 mg Pb l(-1). High concentrations of Pb inhibited the growth of LDF and affected the activity of ligninolytic enzymes, but the extent of inhibition varied among different LDF species. In consequence, Pb contamination in soil may have a negative impact on recycling of organic carbon.
Tuomela
Marja
M
Department of Applied Chemistry and Microbiology, P.O. Box 56, Biocenter 1, University of Helsinki, 00014 Helsinki, Finland. marja.tuomela@helsinki.fi
Steffen
Kari T
KT
Kerko
Elina
E
Hartikainen
Helinä
H
Hofrichter
Martin
M
Hatakka
Annele
A
D000704:Analysis of Variance; D001487:Basidiomycota / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D001673:Biodegradation, Environmental / Q000187:drug effects; D002250:Carbon Radioisotopes / Q000378:metabolism; D005387:Finland; D007854:Lead / Q000633:toxicity*; D008031:Lignin / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000633:toxicity*; D013045:Species Specificity; D013997:Time Factors
D002250:Carbon Radioisotopes; D012989:Soil Pollutants; D007854:Lead; D008031:Lignin; D010544:Peroxidases; C051129:manganese peroxidase