Chromosomal aberrations induced by (12)C6+ ions and 60Co gamma-rays in mouse immature oocytes.
The ovaries of Kun-Ming strain mice (3 weeks) were irradiated with different doses of (12)C6+ ion or (60)Co gamma-ray. Chromosomal aberrations were analyzed in metaphase II oocytes at 7 weeks after irradiation. The relative biological effectiveness (RBE) of (12)C6+ ion was calculated with respect to 60Co gamma-ray for the induction of chromosomal aberrations. The (12)C6+ ion and 60Co gamma-ray dose-response relationships for chromosomal aberrations were plotted by linear quadratic models. The data showed that there was a dose-related increase in frequency of chromosomal aberrations in all the treated groups compared to controls. The RBE values for (12)C6+ ions relative to 60Co gamma-rays were 2.49, 2.29, 1.57, 1.42 or 1.32 for the doses of 0.5, 1.0, 2.0, 4.0 or 6.0 Gy, respectively. Moreover, a different distribution of the various types of aberrations has been found for (12)C6+ ion and 60Co gamma-ray irradiations. The dose-response relationships for (12)C6+ ion and 60Co gamma-ray exhibited positive correlations. The results from the present study may be helpful for assessing genetic damage following exposure of immature oocytes to ionizing radiation.
Zhang
Hong
H
Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China. zhangh@impcas.ac.cn
Duan
Xin
X
Yuan
Zhigang
Z
Li
Wenjian
W
Zhou
Guangming
G
Zhou
Qingming
Q
Bing
Liu
L
Min
Fengling
F
Li
Xiaoda
X
Xie
Yi
Y
D000818:Animals; D002244:Carbon / Q000737:chemistry* / Q000494:pharmacology*; D002869:Chromosome Aberrations / Q000528:radiation effects*; D033481:Chromosomes, Mammalian / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D020450:Heavy Ions; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*
D003037:Cobalt Radioisotopes; D002244:Carbon