Association of 4G/5G polymorphism in PAI1 promoter with PAI1 level in deep vein thrombosis.
OBJECTIVE
To reveal the association of 4G/5G polymorphism in the promoter region of the plasminogen activator inhibitor 1 gene (PAI1) with plasma PAI1 level in deep vein thrombosis (DVT) in Chinese Han ethnic group.


METHODS
One hundred and twenty Chinese DVT patients and 120 healthy controls were recruited. The PAI1 promoter 4G/5G polymorphism was detected using polymerase chain reaction (PCR). The antigen of tissue-type plasminogen activator (tPA) or PAI1 was quantified by a commercially available enzyme-linked immunosorbent assay (ELISA) in DVT cases and health controlsì respectively.


RESULTS
Neither in the distribution of PAI1 promoter 4G/5G polymorphism nor in the frequencies of 4G and 5G allele was there a difference between two groups. The levels of PAI1 antigen in the carriers of the 4G/4G genotype were significantly higher than those either in the 4G/5G genotype or in the 5G/5G genotype; In the 4G/5G genotype or in the 5G/5G genotype the TG levels are an independently determinant factor of PAI1 antigen levels.


CONCLUSIONS
There is a close relationship of the PAI1 4G/5G polymorphism to its plasma level in deep vein thrombosis in Chinese Han ethnic group, although lack of association between this genetic variation and risk of DVT suggest no major cause-effect pathogenic role of this polymorphism by itself.
Chen
Yong-li
YL
Department of Internal Medicine, General Hospital of Tianjin Medical University, Tianjin, P. R. China. chenyongli555@sina.com
Zhang
Jing-xia
JX
Wang
Pei-xian
PX
Cui
Rang-zhuang
RZ
Zhao
Fu-mei
FM
Mao
Yong-min
YM
Li
Jing-yan
JY
Bi
Yan-yong
YY
Li
Hui
H
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004586:Electrophoresis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020246:Venous Thrombosis / Q000097:blood / Q000235:genetics*
D017395:Plasminogen Activator Inhibitor 1