[Isolation, induction and differentiation of human blood-derived endothelia progenitor cells].
OBJECTIVE
To determine the biological traits and optimal condition for the induction and differentiation of endothelial progenitor cells from peripheral blood in healthy adults.


METHODS
Mononuclear cells isolated from peripheral blood of healthy adults were cultured in M199 medium supplemented with VEGF, bFGF, IGF-1, and EGF. The appearing time of cell clusters or spindle-shaped cells was recorded respectively. Attached spindle-shaped cells were detached and labeled with a series of antibodies against blood vessel endothelial-specific markers.


RESULTS
Attached spindle-like cells appeared 4 days after the culture, cell clusters were observed at 5 to 8 days, and cord-like structure was formed by 10th day. These cells expressed endothelial-specific markers.


CONCLUSIONS
Endothelial progenitors cells were derived from mononuclear cells of peripheral blood, which can be induced into endothelial cells at specific conditions.
Liu
Ze-Hao
ZH
Departement of Endocrinology, Xiangya Hospital, Xiangya Hospital, Central South University, Changsha, China. medicalliu@hotmail.com
Lei
Min-Xiang
MX
Wang
Ai-Min
AM
Chen
Zuo-Xiong
ZX
Zhang
Jun
J
Tang
San-E
SE
D002454:Cell Differentiation; D002469:Cell Separation; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*