Analysis of DNA intercalating drugs by TGGE.
Temperature-gradient gel electrophoresis (TGGE) was used to study DNA-drug interactions. The results indicate that at least two classes of DNA intercalating drugs are distinguishable with respect to temperature increase: reversible and irreversible. The method offers an excellent means of visualizing the melting profile of an individual DNA topoisomer in the presence of DNA binding drugs. Our findings coincide with UV/VIS absorption spectroscopy data.
Danko
Patrik
P
Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, P.J. Safárik University, Kosice, Slovakia.
Kozák
Anton
A
Podhradský
Dusan
D
Víglaský
Viktor
V
D055592:Biophysical Phenomena; D001703:Biophysics; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000187:drug effects*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D007364:Intercalating Agents / Q000494:pharmacology*; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000032:analysis / Q000737:chemistry / Q000187:drug effects; D013053:Spectrophotometry; D013696:Temperature
D007364:Intercalating Agents; D004247:DNA