title: Protective effect of isoflurane anesthesia on noise-induced hearing loss in mice.
abstract: OBJECTIVE
  To examine the protective effect of general anesthesia with isoflurane against noise-induced hearing loss in mice.
  
  
  METHODS
  Animal study using noise stimulation and measurement of hearing in BALB/c mice.
  
  
  METHODS
  Mice were exposed to 122 dB peak equivalent sound pressure level click noise for 3 hours per day for 3 consecutive days with or without anesthesia using isoflurane. Hearing levels were measured and hair cell survival ratio was observed.
  
  
  RESULTS
  In mice without anesthesia, hearing threshold increased after noise stimulation (73.7 dB hearing level [HL]) and persisted for at least 1 month. However, in mice exposed to noise under anesthesia, hearing loss was less severe (44.1 dB HL) and had recovered more (26.5 dB HL) by one month. Histological examination showed hair cell survival was higher in anesthetized compared to non-anesthetized mice.
  
  
  CONCLUSIONS
  These data indicate isoflurane general anesthesia protects against noise-induced hearing loss and tissue damage in mice.
authors.affiliation: Department of Anesthesiology and Pain Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
authors.forename: Joung Uk
authors.initials: JU
authors.lastname: Kim
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Hun Hee
authors.initials: HH
authors.lastname: Kang
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Shin
authors.forename: Seung Woo
authors.initials: SW
authors.lastname: Ku
authors.forename: Joong Ho
authors.initials: JH
authors.lastname: Ahn
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jong Woo
authors.initials: JW
authors.lastname: Chung
mesh_terms: D000769:Anesthesia, Inhalation; D018685:Anesthetics, Inhalation / Q000627:therapeutic use*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D005072:Evoked Potentials, Auditory / Q000187:drug effects / Q000502:physiology; D005500:Follow-Up Studies; D006198:Hair Cells, Auditory / Q000473:pathology; D006317:Hearing Loss, Noise-Induced / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D007530:Isoflurane / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D012720:Severity of Illness Index
chemical_list: D018685:Anesthetics, Inhalation; D007530:Isoflurane