Mapping the distribution of 3-hydroxy oxylipins in the ascomycetous yeast Saturnispora saitoi.
The distribution of 3-hydroxy oxylipins in Saturnispora saitoi was mapped using immunofluorescence microscopy. Fluorescence was observed on aggregating ascospores, indicating the presence of 3-hydroxy oxylipins on the surface or between ascospores. The oxylipin was identified as 3-hydroxy 9:1 using gas chromatography mass spectrometry. Furthermore, ultrastructural studies using scanning and transmission electron microscopy on ascospores revealed a clear equatorial ledge surrounding oval-shaped ascospores.
Bareetseng
Andries S
AS
Department of Microbial, Biochemical and Food Biotechnology, UNESCO MIRCEN: Industrial Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, South Africa.
Kock
Johan L F
JL
Pohl
Carolina H
CH
Pretorius
Elma E
EE
Strauss
Catharina J
CJ
Botes
Piet J
PJ
Van Wyk
Pieter W J
PW
Nigam
Santosh
S
D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D004718:Saccharomycetales / Q000737:chemistry* / Q000648:ultrastructure; D013172:Spores, Fungal / Q000737:chemistry / Q000648:ultrastructure
D005227:Fatty Acids