Expanding functionality of RNA: synthesis and properties of RNA containing imidazole modified tandem G-U wobble base pairs.
Imidazole modification at C-5 of uridine that is part of tandem G-U wobble base pairs causes slight reduction of thermal stability (DeltaDeltaG(0)(310) < 0.4 kcal mol(-1)) and relatively small change in hydration of short RNA helices.
Rozners
Eriks
E
Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, USA. e.rozners@neu.edu
Smicius
Romualdas
R
Uchiyama
Chika
C
D020029:Base Pairing; D006150:Guanine Nucleotides / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D044366:Transition Temperature; D014500:Uracil Nucleotides / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D006150:Guanine Nucleotides; D007093:Imidazoles; D014500:Uracil Nucleotides; D014867:Water; D012313:RNA; C029899:imidazole