Characterization of the DNA gyrase from the thermoacidophilic archaeon Thermoplasma acidophilum.
Thermoplasma acidophilum is sensitive to the antibiotic drug novobiocin, which inhibits DNA gyrase. We characterized DNA gyrases from T. acidophilum strains in vitro. The DNA gyrase from a novobiocin-resistant strain and an engineered mutant were less sensitive to novobiocin. The novobiocin-resistant gyrase genes might serve as T. acidophilum genetic markers.
Yamashiro
Kan
K
Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Yamagishi
Akihiko
A
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D027081:DNA Gyrase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004278:DNA, Superhelical / Q000737:chemistry; D004352:Drug Resistance, Microbial / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019766:Genes, Archaeal; D008969:Molecular Sequence Data; D009675:Novobiocin / Q000494:pharmacology; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D017385:Sequence Homology; D013822:Thermoplasma / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
D000900:Anti-Bacterial Agents; D004278:DNA, Superhelical; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D009675:Novobiocin; D027081:DNA Gyrase