Localization of sequences within the latency-related gene of bovine herpesvirus 1 that inhibit mammalian cell growth.
The latency-related (LR) RNA of bovine herpes virus 1 (BHV-1) is abundantly expressed in sensory neurons of latently infected cattle. Wild-type expression of LR gene products is required for the latency-reactivation cycle. LR gene products inhibit apoptosis, bICP0 expression, and mammalian cell growth. The cell growth inhibitory function of the LR gene maps to a 463-bp XbaI-PstI fragment. Introduction of stop codons into the XbaI-PstI fragment had no effect on inhibiting growth. Expression of a LR strand-specific transcript correlates with growth inhibition in bovine fibroblasts and mouse neuroblastoma cells.
Geiser
Vicki
V
Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, and School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68583, USA.
Jones
Clinton
C
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004279:DNA, Viral / Q000032:analysis / Q000494:pharmacology*; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000502:physiology*; D014775:Virus Activation; D017735:Virus Latency / Q000235:genetics*
D004279:DNA, Viral