Identification of class II HLA-DRB1*03-bound measles virus peptides by 2D-liquid chromatography tandem mass spectrometry.
Two-dimensional liquid chromatography (2D-LC), combined with gas phase fractionation tandem mass spectrometry, was used to identify 13 naturally processed peptides originating from measles virus that were presented by HLA-DRB1*03 class II molecules. The peptides are from three of the six measles structural proteins: phosphoprotein, nucleocapsid, and hemagglutinin. These peptides provide an important first step toward understanding the mechanism of immune response to measles virus and development of a new generation of peptide-based vaccines.
Johnson
Kenneth L
KL
Mayo Proteomics Research Center, Mayo Vaccine Research Group, Program in Translational Immunovirology and Biodefense, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA.
Ovsyannikova
Inna G
IG
Poland
Gregory A
GA
Muddiman
David C
DC
D000595:Amino Acid Sequence; D019496:Cancer Vaccines / Q000737:chemistry; D002412:Cations; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods*; D016208:Databases, Factual; D030562:Databases, Protein; D006680:HLA Antigens / Q000737:chemistry; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006388:Hemagglutinins / Q000737:chemistry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D008458:Measles Vaccine / Q000737:chemistry; D008459:Measles virus / Q000235:genetics*; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000235:genetics*; D010750:Phosphoproteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
D019496:Cancer Vaccines; D002412:Cations; D006680:HLA Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D006388:Hemagglutinins; D008458:Measles Vaccine; D019590:Nucleocapsid Proteins; D010455:Peptides; D010750:Phosphoproteins