Epstein-Barr virus-infected Akata cells are sensitive to histone deacetylase inhibitor TSA-provoked apoptosis.
Epstein-Barr virus (EBV) infects more than 90 % of the world's population and has a potential oncogenic nature. A histone deacetylase (HDAC) inhibitor, trichostatin A (TSA), has shown potential ability in cancer chemoprevention and treatment, but its effect on EBV-infected Akata cells has not been examined. This study investigated the effect of TSA on the proliferation and apoptosis of the cells. TSA inhibited cell growth and induced cytotoxicity in the EBV-infected Akata cells. TSA treatment sensitively induced apoptosis in the cell, which was demonstrated by the increased number of positively stained cells in the TUNEL assay, the migration of many cells to the sub-G0/G1 phase in flow cytometric analysis, and the ladder formation of genomic DNA. Western blot analysis showed that caspase-dependent pathways are involved in the TSA-induced apoptosis of EBV-infected Akata cells. Overall, this study shows that EBV-infected B lymphomas are quite sensitive to TSA-provoked apoptosis.
Kook
Sung-Ho
SH
Laboratory of Cell Biology in Department of Orthodontics and Institute of Oral Biosciences, Chonbuk National University, Chonju 561-756, Korea.
Son
Young-Ok
YO
Han
Seong-Kyu
SK
Lee
Hyung-Soon
HS
Kim
Beom-Tae
BT
Jang
Yong-Suk
YS
Choi
Ki-Choon
KC
Lee
Keun-Soo
KS
Kim
So-Soon
SS
Lim
Ji-Young
JY
Jeon
Young-Mi
YM
Kim
Jong-Ghee
JG
Lee
Jeong-Chae
JC
D017209:Apoptosis; D053181:Caspase 8 / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D056572:Histone Deacetylase Inhibitors; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D019169:Jurkat Cells; D011419:Propidium / Q000494:pharmacology
D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D011419:Propidium; C012589:trichostatin A; D004247:DNA; D053181:Caspase 8