title: Characterization of the DNA gyrase from the thermoacidophilic archaeon Thermoplasma acidophilum.
abstract: Thermoplasma acidophilum is sensitive to the antibiotic drug novobiocin, which inhibits DNA gyrase. We characterized DNA gyrases from T. acidophilum strains in vitro. The DNA gyrase from a novobiocin-resistant strain and an engineered mutant were less sensitive to novobiocin. The novobiocin-resistant gyrase genes might serve as T. acidophilum genetic markers.
authors.affiliation: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Kan
authors.initials: K
authors.lastname: Yamashiro
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yamagishi
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D027081:DNA Gyrase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004278:DNA, Superhelical / Q000737:chemistry; D004352:Drug Resistance, Microbial / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019766:Genes, Archaeal; D008969:Molecular Sequence Data; D009675:Novobiocin / Q000494:pharmacology; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D017385:Sequence Homology; D013822:Thermoplasma / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D004278:DNA, Superhelical; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D009675:Novobiocin; D027081:DNA Gyrase