title: EcoRI polymorphism of the metastasis-suppressor gene NME1 in Mexican patients with breast cancer.
abstract: Human breast cancer cells with high metastatic potential show reduced expression of the metastasis-suppressor gene NME1. There are two polymorphic sites for the restriction enzymes BglII and EcoRI, both detectable by Southern blot analysis. Although the BglII site has been analyzed for loss of heterozygosity, the biallelic EcoRI site polymorphism has not been studied in association with breast cancer, complications or metastasis. We analyzed EcoRI site allele frequencies in Mexican patients with breast cancer, using polymerase chain reaction -restriction fragment length polymorphisms. The polymorphic allelic frequencies in the cases and reference groups were 0.4215 and 0.3375, respectively; this difference was not statistically significant (chi2=0.8687, p=0.3512). Thus, EcoR1 polymorphic site was not associated with breast cancer in this series, but could be analyzed in association with metastases and might be informative in the evaluation of loss of heterozygosity in women with breast cancer.
authors.affiliation: Medicina Molecular Division, Centro de Investigacion Biomedica de Occidente, Guadalajara, Jalisco, Mexico. suanguru@yahoo.com.mx
authors.forename: Susan Andrea Gutierrez
authors.initials: SA
authors.lastname: Rubio
authors.forename: Silvia Esperanza Flores
authors.initials: SE
authors.lastname: Martinez
authors.forename: Jose Sanchez
authors.initials: JS
authors.lastname: Corona
authors.forename: Adriana Patricia Mendizabal
authors.initials: AP
authors.lastname: Ruiz
authors.forename: Angel Emilio Suarez
authors.initials: AE
authors.lastname: Rincon
authors.forename: Ignacio Arevalo
authors.initials: IA
authors.lastname: Lagunas
authors.forename: Jose Gonzalo Vazquez
authors.initials: JG
authors.lastname: Camacho
authors.forename: Maria Cristina Moran
authors.initials: MC
authors.lastname: Moguel
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D008800:Mexico; D009362:Neoplasm Metastasis; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000032:analysis; D044465:White People / Q000235:genetics
chemical_list: D012333:RNA, Messenger