Transgenic potato expressing Abeta reduce Abeta burden in Alzheimer's disease mouse model.
Beta amyloid (Abeta) is believed one of the major pathogens of Alzheimer's disease (AD), and the reduction of Abeta is considered a primary therapeutic target. Immunization with Abeta can reduce Abeta burden and pathological features in transgenic AD model mice. Transgenic potato plants were made using genes encoding 5 tandem repeats of Abeta1-42 peptides with an ER retention signal. Amyloid precursor protein transgenic mice (Tg2576) fed with transgenic potato tubers with adjuvant showed a primary immune response and a partial reduction of Abeta burden in the brain. Thus, Abeta tandem repeats can be expressed in transgenic potato plants to form immunologically functional Abeta, and these potatoes has a potential to be used for the prevention and treatment of AD.
Youm
Jung Won
JW
Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-335, Republic of Korea.
Kim
Hee
H
Han
Jee Hye Lo
JH
Jang
Chang Hwan
CH
Ha
Hee Jin
HJ
Mook-Jung
Inhee
I
Jeon
Jae Heung
JH
Choi
Cheol Yong
CY
Kim
Young Ho
YH
Kim
Hyun Soon
HS
Joung
Hyouk
H
D000544:Alzheimer Disease / Q000235:genetics* / Q000473:pathology / Q000517:prevention & control*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D001921:Brain / Q000473:pathology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007114:Immunization; D007532:Isoleucine / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D058225:Plaque, Amyloid / Q000235:genetics / Q000473:pathology; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism; D020080:Tandem Repeat Sequences / Q000235:genetics; D013997:Time Factors; D014612:Vaccines / Q000627:therapeutic use
D016229:Amyloid beta-Peptides; D014612:Vaccines; D007532:Isoleucine