Propionate-regulated high-yield protein production in Escherichia coli.
A new expression system containing the Salmonella enterica prpBCDE promoter (P(prpB)) responsible for expression of the propionate catabolic genes (prp BCDE) and prpR encoding the positive regulator of this promoter has been developed and tested. The main features of the expression system compared to those based on the bacteriophage T7 promoter are low background expression and high induced expression in Escherichia coli strains BL21, BL21(DE3), MG1655, and W3110. In addition, propionate is an inexpensive, simple-to-use, nontoxic inducer that is attractive for large-scale protein production. Hence, this new system is highly complementary to the widely used T7 promoter-driven expression systems.
Lee
Sung Kuk
SK
Department of Chemical Engineering and Bioengineering, University of California, Synthetic Biology Department, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Keasling
Jay D
JD
D017123:Bacteriophage T7 / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D009875:Operator Regions, Genetic / Q000235:genetics; D009876:Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011422:Propionates / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D019779:Salmonella enterica / Q000235:genetics
D011422:Propionates; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins