Inhibition of vascular smooth muscle cell proliferation in vitro by genetically engineered marrow stromal cells secreting calcitonin gene-related peptide.
Calcitonin gene-related peptide (CGRP) has a beneficial effect in pulmonary hypertension and is a target for cardiovascular gene therapy. Marrow stromal cells (MSCs), also known as mesenchymal stem cells, hold promise for use in adult stem cell-based ex vivo gene therapy. To test the hypothesis that genetically engineered MSCs secreting CGRP can inhibit vascular smooth muscle cell proliferation, rat MSCs were isolated, ex vivo expanded, and transduced with adenovirus containing CGRP. Immunocytochemical analysis demonstrated that wild type rat MSCs express markers specific for stem cells, endothelial cells, and smooth muscle cells including Thy-1, c-Kit, von Willebrand Factor and alpha-smooth muscle actin. Immunocytochemistry confirmed the expression of CGRP by the transduced rat MSCs. The transduced rat MSCs released 10.3+/-1.3 pmol CGRP/1 x 10(6) cells/48 h (mean+/-S.E.M., n=3) into culture medium at MOI 300 and the CGRP-containing culture supernatant from the transduced cells inhibited the proliferation of rat pulmonary artery smooth muscle cells (PASMCs) and rat aortic smooth muscle cells (ASMCs) in culture. Co-culture of the transduced rat MSCs with rat PASMCs or rat ASMCs also inhibited smooth muscle cell proliferation. These findings suggest that this novel adult stem cell-based CGRP gene therapy has potential for the treatment of cardiovascular diseases including pulmonary hypertension.
Deng
Weiwen
W
Department of Pharmacology, SL 83, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112, USA.
St Hilaire
Rose-Claire
RC
Chattergoon
Natasha N
NN
Jeter
James R
JR
Kadowitz
Philip J
PJ
D000199:Actins / Q000378:metabolism; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D018920:Coculture Techniques; D005818:Genetic Engineering; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D051381:Rats; D011914:Rats, Inbred BN; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells; D018800:Thy-1 Antigens / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
D000199:Actins; D018800:Thy-1 Antigens; D014841:von Willebrand Factor; D019009:Proto-Oncogene Proteins c-kit; D015740:Calcitonin Gene-Related Peptide