[Morphological observation of metaphase I arrested oocytes in IVF-ET cycles].
OBJECTIVE
To observe morphological characteristics and in-vitro maturation ratio of all metaphase I arrested oocytes in IVF-ET cycles.


METHODS
In two IVF-ET cycles, maturation culture in vitro was performed and we evaluated morphological characteristics of all oocytes arrested in metaphase I.


RESULTS
Metaphase I arrested oocytes presented with uniform cytoplasm, compact zone and no space between oocyte and zone. Incompact association was observed between granule cell and zone without radiated structures. There was no markedly alterations in morphological characteristics after 24, 48, 72 hours of maturation culture, respectively. All oocytes were arrested in Metaphase I and no polar growed.


CONCLUSIONS
All metaphase I arrested oocytes possess of unique morphological characteristics and fail to mature following current culture assay in IVF-ET cycles.
Zhang
Ning-Yuan
NY
Reproduction Medicine Center, Department of Obstetrics & Gynecology, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Jiangsu Nanjing 210008.
Hu
Ya-Li
YL
Sun
Hai-Xiang
HX
Wang
Bin
B
Xu
Zhi-Peng
ZP
D000328:Adult; D002478:Cells, Cultured; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008677:Metaphase; D009865:Oocytes / Q000648:ultrastructure*