Neutralization of HIV-1 primary isolate by ELDKWA-specific murine monoclonal antibodies.
ELDKWA on HIV-1 gp41 is a conserved epitope recognized by one broadly neutralizing monoclonal antibody 2F5, which is a promising candidate target for vaccine design. Here we report two ELDKWA-specific monoclonal antibodies (mAbs), 18F11 and 7E10, that were screened from the splenocytes of mice immunized by recombinant GST-(ELDKWA)4 protein. In further evaluation, these mAbs exhibited appreciable neutralizing activities against HIV-1 primary isolate 92US675 (clade B) with IC50 (50% inhibition concentration) of 6.84 +/- 0.36 microg/ml and 10.66 +/- 1.69 microg/ml, respectively. Unexpectedly, neither of these two murine mAbs could neutralize laboratory-adapted strain HIV-1 IIIB (clade B). As a control, human mAb 2F5 neutralized both primary and laboratory-adapted strains. These data strongly suggest that ELDKWA-specific antibodies induced by different antigenic formats show different neutralizing activities against HIV-1, which implies another complication in the development of effective vaccines.
Zhang
Geng
G
Laboratory of Immunology, Department of Biology, Tsinghua University, Protein Science Laboratory of MOE, Beijing, P R China.
Lu
Hong
H
Lu
Yun
Y
Jiang
ShiBo
S
Chen
Ying-Hua
YH
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002460:Cell Line; D006224:Cricetinae; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D015483:HIV Antibodies / Q000276:immunology* / Q000302:isolation & purification; D015497:HIV-1 / Q000737:chemistry* / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015483:HIV Antibodies