[Determination of vitamins D2, vitamin D3 in cosmetics by high performance liquid chromatography].
OBJECTIVE
A high performance liquid chromatography method was used to detect vitamins D2 and vitamin D3, which is useful to know the use of vitamins D2 and vitamin D3 in cosmetics, prohibit the influx of cosmetics containing vitamins D2 and vitamin D3 to cosmetic market, safeguard the health of consumers.


METHODS
A high performance liquid chromatography method was established for determination of vitamins D2 and vitamin D3 in cosmetics. The separation condition was optimized by trying different type of columns and mobile phases.


RESULTS
The experiment goes on a Alltima C18 column (250 mm x 4.6 mm I. D., 5 microm)using methanol-acetonitrile (90: 10) as mobile phase at a flow rate of 1.0 ml/min, with the column temperature 25 degrees C and detection wave 265nm. The liner range is from 0.5 mg/L to 100 mg/L with good relationship. The detection limit of vitamin D2 is 0. 12 mg/L, the precision is less than 3.8% and recovery varies from 94.2% to 101.4%, while the detection limit of vitamin D3 is 0.06 mg/L, the precision is less than 3.5% and recovery varies from 91.6% to 97.2%.


CONCLUSIONS
The method is simple, precise and accurate, which is suitable for the determination of vitamins D2 and vitamin D3 in cosmetics.
Zhu
Ying
Y
Institute for Environmental Hygiene and Health Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing, China.
Yang
Yan-Wei
YW
Wang
Xin
X
D002762:Cholecalciferol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003358:Cosmetics / Q000737:chemistry*; D004872:Ergocalciferols / Q000032:analysis*; D012680:Sensitivity and Specificity
D003358:Cosmetics; D004872:Ergocalciferols; D002762:Cholecalciferol