HO-1 induction in the guinea-pig stomach: protection of smooth muscle functional performance during cobalt-induced oxidative stress.
Distribution and enzymatic activity of haem oxygenase (HO) was investigated in the stomach of healthy guinea pigs or animals subjected to in vivo cobalt-induced oxidative stress. The physiological role of HO-1 and HO-2 isoenzymes in fundic and antral area of the stomach was assessed by studying the action of HO substrate--hemin--on ionic currents of single smooth muscle cells. The data obtained suggest that HO-1 induction might serve in the guinea-pig stomach as genetically determined defense mechanism aimed to combat toxic stress-related pathology in order to preserve the functional performance of the organ.
Duridanova
D B
DB
Institute of Biophysics, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., bldg. 21, 1113 Sofia, Bulgaria. dessybd@bio.bas.bg
Gagov
H S
HS
Bolton
T B
TB
D000818:Animals; D003035:Cobalt / Q000494:pharmacology; D004594:Electrophysiology; D015971:Gene Expression Regulation, Enzymologic; D006168:Guinea Pigs; D006419:Heme Oxygenase (Decyclizing) / Q000502:physiology; D051547:Heme Oxygenase-1 / Q000235:genetics* / Q000502:physiology; D006427:Hemin / Q000494:pharmacology; D008297:Male; D009130:Muscle, Smooth / Q000166:cytology / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D013270:Stomach / Q000201:enzymology*
D003035:Cobalt; D006427:Hemin; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C105245:heme oxygenase-2; D011188:Potassium