Detection of DNA of W. bancrofti in blood samples by QC-PCR-ELISA-based.
An internal control was used in a polymerase chain reaction PCR-ELISA-based technique to detect the DNA repeat of the filarial parasite W. bancrofti. The sensitivity of the test could detect as low as one single microfilaria added to 200 ul of blood. The assay was evaluated on field samples from persons living in areas endemic for filariasis. Examination of night blood of 113 individuals for the presence of microfilaria by filtration revealed 44 microfilaria carriers. All microfilaria carriers were positive in the PCR-ELISA and, in addition, 14 more samples were proven to contain parasite DNA. All the 58 proven cases had circulating filarial antigens in their serum samples. Assuming a sensitivity of PCR-ELISA on night blood of 100%, the sensitivity of night blood filtration was 74% and that of circulating filarial antigens is 100%. The data showed that the described PCR-ELISA method was capable of detecting the filarial infections. Consequently, this method facilitated the identification of the filarial endemic areas and the monitoring of control programs.
Hassan
Mohsen
M
Department of Parasitology, Faculty of Medicine, University of Zagazig, Egypt.
Sanad
Magda M
MM
el-Karamany
Iman
I
Abdel-Tawab
Mohamed
M
Shalaby
Mohsen
M
el-Dairouty
Aly
A
Assal
Khalid
K
Gamal-Edin
Mohamed Kamel F
MK
Adel el-Kadi
Mohammad
M
D000818:Animals; D018509:DNA, Helminth / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005368:Filariasis / Q000175:diagnosis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014958:Wuchereria bancrofti / Q000276:immunology / Q000302:isolation & purification*
D018509:DNA, Helminth