Molecules contributing to the maintenance of periodontal tissues. Their possible association with orthodontic tooth movement.
This review is aimed at providing a depiction of molecules and their topography which characterize native gingiva and PDL fibroblasts, to describe their function in tissue maintenance, and to discuss their possible modulation due to orthodontic tooth movement. Maintenance of the human periodontium requires the balance of proliferation and differentiation in the respective tissues' cells. Moreover, the cells must synthesize the extracellular matrix molecules and receptors that facilitate adhesion. To describe the molecules that contribute to periodontal tissue maintenance, we illustrate the localization of their expression and topography on frozen sections from native gingival tissue and primary cell cultures derived from periodontal ligament. In native gingival epithelium, proliferation is confined to basal and parabasal cells. Keratin K14, when used as structural marker, is visible in the entire epithelium, while K13, an indicator of early differentiation, is restricted to the suprabasal cell compartment. Vimentin indicates mesenchymal cells in the subgingival connective tissue. Concerning the matrix molecules, collagen type-IV is abundant at the epithelium-lamina propria interface, and fibronectin is apparent throughout the mesenchyme. The matrix receptor integrin beta1 reveals a pericellular localization in basal and parabasal cells, while focal adhesion kinase p125FAK is seen pericellularly in all epithelial layers. Cultures of primary periodontal ligament (PDL) fibroblasts (PDL-F) reveal expression of vimentin, strong proliferation, synthesis and extracellular deposition of collagen type-I and fibronectin. The integrin subunits beta1 and p125(FAK) are largely detectable at the cell periphery.
Müssig
Eva
E
Department of Orthodontics and Dentofacial Orthopedics, Dental School, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany. Eva.Muessig@med.uni-heidelberg.de
Tomakidi
Pascal
P
Steinberg
Thorsten
T
D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D010519:Periodontium / Q000166:cytology* / Q000502:physiology*; D014018:Tissue Distribution; D014087:Tooth Movement Techniques / Q000379:methods*
D016326:Extracellular Matrix Proteins