Emerging challenges in ligand discovery: new opportunities for chromatographic assay.
Ligand discovery initiatives are facing interesting challenges as ever-increasing numbers of proteins are entering screening programs. As an answer to steady pressure to improve performance in drug discovery, ligand discovery can expect to play an expanded role in generating small molecules as probes to help uncover the function of novel proteins. Chromatographic assay formats can offer new entry points into standard interaction characterization (binding and rate constants) as well as powerful, scaleable methods for compound screening. This review presents recent advancements in chromatographic assay technology, with a particular focus on frontal affinity chromatography as a platform technology for interaction analysis.
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University of Calgary, SAMS Centre for Proteomics, Department of Biochemistry & Molecular Biology, Health Sciences Center, 3330 Hospital Drive NW, Calgary, Alberta, T2N 4N1, Canada. dschriem@ucalgary.ca
Schriemer
David C
DC
D002845:Chromatography / Q000379:methods*; D016678:Genome / Q000235:genetics; D006801:Humans; D008024:Ligands; D013058:Mass Spectrometry; D020349:Surface Plasmon Resonance
D008024:Ligands