title: Chemically modified heparin inhibits the in vitro adhesion of nonsmall cell lung cancer cells to P-selectin.
abstract: OBJECTIVE
  Several independent studies have indicated that tumor metastasis can be inhibited by chemically modified heparin with low anticoagulant activity in the different tumor models. The mechanism of inhibition by the heparin derivatives in part accounts for the interference of tumor cell-platelet interaction mediated by P-selectin.
  
  
  METHODS
  In the present study, we demonstrated that both heparin and chemically modified heparins inhibited the adhesion of nonsmall cell lung cancer (NSCLC) cells to P-selectin under static or flow conditions in vitro.
  
  
  RESULTS
  Flow cytometric analysis with the heparan sulfate-specific monoclonal antibody revealed that both NSCLC cells express heparan sulfate-like proteoglycans. Furthermore, heparinase treatment impaired P-selectin binding, indicating that heparan sulfate-like proteoglycans on the tumor cell surface are implicated in the adhesion of NSCLC cells to P-selectin.
  
  
  CONCLUSIONS
  These findings suggest that some chemically modified heparins with low anticoagulant activity may deserve further testing in the experimental NSCLC treatment protocols.
authors.affiliation: Institute of Genetics and Cytology, School of Life Science, Northeast Normal University, Changchun, 130024, Jilin, People's Republic of China.
authors.forename: Yanguang
authors.initials: Y
authors.lastname: Gao
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.forename: Sheng
authors.initials: S
authors.lastname: Zheng
authors.forename: Xueqing
authors.initials: X
authors.lastname: Ba
authors.forename: Shui
authors.initials: S
authors.lastname: Hao
authors.forename: Xianlu
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D016466:CHO Cells; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004353:Drug Evaluation, Preclinical; D006493:Heparin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness; D019007:P-Selectin / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; C520325:N-desulfated,2-O,3-O-desulfated heparin; D019007:P-Selectin; C504902:RO-heparin; C044153:heparin, N-desulfated; C410523:heparin, O-desulfated; D006493:Heparin