title: [Expression of muscarinic acetylcholine receptor-1 in human scleral fibroblasts].
abstract: OBJECTIVE
  The aim of this study was to identify the presence of muscarinic acetylcholine receptors-1 (mAChRs-1) in human sclera in order to determine whether the sclera is a potential site of action for mAChR antagonists.
  
  
  METHODS
  Cell lines of human scleral fibroblasts were cultured in Dulbecco Modified Eagle's Medium. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was used to detect mRNA expression of M1 receptors in the fibroblasts. Immunocytochemistry was used to detect proteins of mAChRs in the cell lines. Immunohistochemical study was used to further detect the presence of M1 receptor in the frozen scleral sections.
  
  
  RESULTS
  The cultured fibroblasts demonstrated mRNA expression of M1 receptor in RT-PCR. Protein of the M1 was present in the fibroblasts by examination of immunocytochemistry and immunohistochemistry staining.
  
  
  CONCLUSIONS
  This study demonstrated the presence of M1 receptor in human scleral fibroblasts at both mRNA and protein levels. This finding indicates that the sclera is a potential site of action for the currently used mAChR antagonists in prevention of human myopia.
authors.affiliation: School of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou 325003, China.
authors.forename: Xiang-tian
authors.initials: XT
authors.lastname: Zhou
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Lü
authors.forename: Ruo-zhong
authors.initials: RZ
authors.lastname: Xie
authors.forename: Dan-ning
authors.initials: DN
authors.lastname: Hu
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Fu
authors.forename: Jia
authors.initials: J
authors.lastname: Qu
mesh_terms: D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D043643:Receptor, Muscarinic M1 / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012590:Sclera / Q000166:cytology / Q000378:metabolism*
chemical_list: D043643:Receptor, Muscarinic M1