title: The garlic ingredient diallyl sulfide inhibits cytochrome P450 2E1 dependent bioactivation of acrylamide to glycidamide.
abstract: Genotoxic effects of acrylamide are supposed to result from oxidative biotransformation to glycidamide. After incubation of rat liver slices with acrylamide we detected free glycidamide using a liquid chromatography tandem mass spectrometric method. Glycidamide formation was diminished in the presence of the cytochrome P450 2E1 inhibitor diallyl sulfide (DAS), which is a specific ingredient of garlic. This may be relevant to human health since the suggested carcinogenic risk of dietary acrylamide may be reduced by concomitant intake of garlic.
authors.affiliation: Department of Pharmacology, University Hospital of Cologne, Gleueler Str. 24, D-50931 Cologne, Germany. dirk.taubert@medizin.uni-koeln.de
authors.forename: Dirk
authors.initials: D
authors.lastname: Taubert
authors.forename: Reinhild
authors.initials: R
authors.lastname: Glöckner
authors.forename: Dieter
authors.initials: D
authors.lastname: Müller
authors.forename: Edgar
authors.initials: E
authors.lastname: Schömig
mesh_terms: D020106:Acrylamide / Q000378:metabolism* / Q000493:pharmacokinetics; D000498:Allyl Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001711:Biotransformation / Q000187:drug effects; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism* / Q000493:pharmacokinetics; D005737:Garlic / Q000737:chemistry*; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013440:Sulfides / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000498:Allyl Compounds; D004852:Epoxy Compounds; D013440:Sulfides; D020106:Acrylamide; C038491:allyl sulfide; C071834:glycidamide; D019392:Cytochrome P-450 CYP2E1