[Dependence of the genotypic characteristics of Acidithiobacillus ferrooxidans on the physical, chemical, and electrophysical properties of pyrites].
This study focused on the effect of physical, chemical, and electrophysical properties of two pyrites, pyrite 1, which had hole-type (p-type) conductivity, and pyrite 2, with electron-type (n-type) conductivity, on the genotypic characteristics of Acidithiobacillus ferrooxidans strains TFV-1 and TFBk, which were isolated from different substrates. After the adaptation of the strains to the pyrites at a pulp density of 1%, pulsed-field electrophoresis revealed changes in the chromosomal DNA of strain TFV-1 adapted to pyrite 1 and strain TFBk adapted to either of the pyrite types. In pyrite-adapted strain TFBk, the plasmid composition was the same as after growth on a medium containing ferrous iron, whereas, in strain TFV-1, changes in plasmid sizes or both in plasmid sizes and plasmid number occurred. After an increase in the density of the pyrite 2 pulp from 1 to 10%, the plasmid number increased from three to four, and, after an increase in the density of the pyrite 1 pulp from 1 to 7%, the plasmid number increased from two to six.
Tupikina
O V
OV
Kondrat'eva
T F
TF
Karavaĭko
G I
GI
D042763:Acidithiobacillus / Q000235:genetics* / Q000502:physiology; D000222:Adaptation, Physiological; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004553:Electric Conductivity; D007501:Iron / Q000737:chemistry* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D013045:Species Specificity; D013440:Sulfides / Q000737:chemistry* / Q000378:metabolism*
D004269:DNA, Bacterial; D013440:Sulfides; C011342:pyrite; D007501:Iron