Novel and simple ELISA-based method for antibody affinity determination.
New coordinates for antibody affinity determination by ELISA are suggested. The suggested approach is very simple but at the same time it is more convenient and is deprived of the drawbacks inhered in the earlier suggested methods. The examples of antibody affinity determination by the suggested methods for both simulative and experimental binding curves are considered. It was demonstrated that the suggested methods allow getting more precise values of antibody affinity.
Bobrovnik
S A
SA
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv. sab@biochem.kiev.ua
Komisarenko
S V
SV
Ilyina
L V
LV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D018448:Models, Immunological
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex