Purification of phocid herpesvirus type 1 glycoproteins B and D and pilot studies of immunogenicity in mice.
In the process of developing a subunit vaccine against phocid herpesvirus type 1, we have cloned and expressed the glycoproteins B and D (gB and gD) of phicid herpesvirus type 1, using an eukaryotic baculovirus expression system. To establish the proof of concept, candidate iscom vaccines based on these affinity-purified proteins either alone or in combination, were tested for their immunogenicity in BALB/C mice. Mice immunised with a combination of gB and gD developed higher antibody and proliferative T cell responses against PhHV-1 than those immunised with gB or gD alone. The corresponding antibody and T cell proliferative responses were higher against PhHV-1 than against FHV. These data favour further testing of these candidate vaccines based on gB and gD in the FHV-cat model.
Martina
B E E
BE
Seal Rehabilitation and Research Centre, Hoofdstraat 94a, 9968 AG Pieterburen, The Netherlands.
Harder
T C
TC
Osterhaus
A D M E
AD
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016367:Baculoviridae / Q000235:genetics; D005260:Female; D022283:Herpesvirus Vaccines / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D022283:Herpesvirus Vaccines; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C488985:glycoprotein D, Phocid herpesvirus 1