title: Decavanadate interacts with microsomal NADH oxidation system and enhances cytochrome c reduction.
abstract: Oxidation of NADH with accompanying oxygen consumption (NADH:O(2) = 1:1) was observed in the combined presence of metavanadate (MV), decavanadate (DV) and microsomes. Oxygen consumption was negligible in the absence of MV, but NADH was oxidized and DV was reduced to a form of vanadyl-V(IV), colored blue like vanadyl sulfate but differed from it in having a 23-fold higher absorbance at 700 nm. DV can interact with the NADH oxidation system of microsomes as an electron acceptor, in addition to the known ferricyanide and cytochrome c. DV enhances rate of cytochrome c reduction significantly at microM concentrations. These studies indicate potential of DV as a redox intermediate.
authors.affiliation: Centre for DNA Fingerprinting & Diagnostics, Hyderabad 500 076, India. trs@cdfd.org.in
authors.forename: T
authors.initials: T
authors.lastname: Ramasarma
authors.forename: Aparna V S
authors.initials: AV
authors.lastname: Rao
mesh_terms: D000818:Animals; D045304:Cytochromes c / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology*; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D014638:Vanadates / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D009243:NAD; D014638:Vanadates; D045304:Cytochromes c