title: Aberrant promoter methylation of EDNRB in lung cancer in Taiwan.
abstract: Hypermethylation has been shown in the promoter region of the endothelin receptor B (EDNRB) gene in several human tumors, but its role in lung cancer formation is unclear. In this study, genomic DNA from lung cancer patients was subjected to methylation-specific PCR to determine the methylation status of the EDNRB gene in lung cancer. Aberrant methylation of the EDNRB gene was detected in 32.9% (26 of 79) lung cancer patients. Promoter hypermethylation of EDNRB was found to significantly differ with histological type but was not correlated to other clinicopathological characteristics. Decreased mRNA transcripts were correlated to aberrant methylation. Treatment with 5-aza-deoxycytidine reversed the methylation status and re-expression of the EDNRB gene in the H1355 human lung cancer cell line. Our results suggest that inactivation of the EDNRB gene through epigenetic alteration is highly prevalent in lung cancer in Taiwan.
authors.affiliation: Institute of Medicine and School of Nurse, Chung-Shan Medical University, Taichung, Taiwan.
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Chen
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Lin
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Chen
authors.forename: Hsin-Yuan
authors.initials: HY
authors.lastname: Fang
authors.forename: Ching-Yuan
authors.initials: CY
authors.lastname: Cheng
authors.forename: Chih-Wen
authors.initials: CW
authors.lastname: Chang
authors.forename: Ruei-A
authors.initials: RA
authors.lastname: Chen
authors.forename: Hui-Lung
authors.initials: HL
authors.lastname: Tai
authors.forename: Chien-Hsing
authors.initials: CH
authors.lastname: Lee
authors.forename: Ming-Chih
authors.initials: MC
authors.lastname: Chou
authors.forename: Torng-Sen
authors.initials: TS
authors.lastname: Lin
authors.forename: Li-Sung
authors.initials: LS
authors.lastname: Hsu
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D019175:DNA Methylation / Q000187:drug effects; D000077209:Decitabine; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D011401:Promoter Regions, Genetic / Q000235:genetics*; D044023:Receptor, Endothelin B / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013624:Taiwan
chemical_list: D044023:Receptor, Endothelin B; D000077209:Decitabine; D001374:Azacitidine