title: Radiation protection of DNA by ferulic acid under in vitro and in vivo conditions.
abstract: The effect of ferulic acid was studied on gamma-radiation-induced relaxation of plasmid pBR322 DNA and induction of DNA strand breaks in peripheral blood leukocytes and bone marrow cells of mice exposed to whole body gamma-radiation. Presence of 0.5 mM ferulic acid significantly inhibited the disappearance of supercoiled (ccc) plasmid pBR322 with a dose modifying factor (DMF) of 2.0. Intraperitoneal administration of different amounts (50, 75 and 100 mg/kg body weight) of ferulic acid 1 h prior to 4 Gy gamma-radiation exposure showed dose-dependent decrease in the yield of DNA strands breaks in murine peripheral blood leukocytes and bone marrow cells as evidenced from comet assay. The dose-dependent protection was more pronounced in bone marrow cells than in the blood leukocytes. It was observed that there was a time-dependent disappearance of radiation induced strand breaks in blood leukocytes (as evidenced from comet parameters) following whole body radiation exposure commensuration with DNA repair. Administration of 50 mg/kg body weight of ferulic acid after whole body irradiation of mice resulted disappearance of DNA strand breaks at a faster rate compared to irradiated controls, suggesting enhanced DNA repair in ferulic acid treated animals.
authors.affiliation: Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, India.
authors.forename: Dharmendra Kumar
authors.initials: DK
authors.lastname: Maurya
authors.forename: Veena Prakash
authors.initials: VP
authors.lastname: Salvi
authors.forename: Cherupally Krishnan Krishnan
authors.initials: CK
authors.lastname: Nair
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects; D020552:Comet Assay; D003373:Coumaric Acids / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects*; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects*; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays; D007962:Leukocytes / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D010957:Plasmids / Q000187:drug effects / Q000528:radiation effects
chemical_list: D003373:Coumaric Acids; D004247:DNA; C004999:ferulic acid