DSD--an integrated, web-accessible database of Dehydrogenase Enzyme Stereospecificities.
BACKGROUND
Dehydrogenase enzymes belong to the oxidoreductase class and utilise the coenzymes NAD and NADP. Stereo-selectivity is focused on the C4 hydrogen atoms of the nicotinamide ring of NAD(P). Depending upon which hydrogen is transferred at the C4 location, the enzyme is designated as A or B stereospecific.


METHODS
The Dehydrogenase Stereospecificity Database v1.0 (DSD) provides a compilation of enzyme stereochemical data, as sourced from the primary literature, in the form of a web-accessible database. There are two search engines, a menu driven search and a BLAST search. The entries are also linked to several external databases, including the NCBI and the Protein Data Bank, providing wide background information. The database is freely available online at: http://www.jenner.ac.uk/DSD/.


CONCLUSIONS
DSD is a unique compilation available on-line for the first time which provides a key resource for the comparative analysis of reductase hydrogen transfer stereospecificity. As databases increasingly form the backbone of science, largely complete databases such as DSD, are a vital addition.
Toseland
Christopher P
CP
The Jenner Institute, University of Oxford, Compton, Berkshire, RG20 7NN, UK.
McSparron
Helen M
HM
Flower
Darren R
DR
D019295:Computational Biology / Q000379:methods*; D016208:Databases, Factual; D030561:Databases, Nucleic Acid; D030562:Databases, Protein; D004798:Enzymes / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry; D007255:Information Services; D016247:Information Storage and Retrieval; D020407:Internet; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics*; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry; D013237:Stereoisomerism; D013379:Substrate Specificity
D004798:Enzymes; D011506:Proteins; D006859:Hydrogen; D010088:Oxidoreductases