Uptake of liposomes by cultured cardiomyocytes.
Small unilamellar liposomes (SUV) of different phospholipid/polymer composition were labeled with NBD-PC, which served as a bilayersituated fluorescence marker. Neonatal cardiomyocytes were incubated with liposomes and then the cell-associated fluorescence was measured. The factors influencing the liposome uptake by cardiomyocytes such as concentration of lipid, time of incubation, membrane fluidity of liposomes, charge lipid/polymer modification of liposomes and anoxia of cultured cardiomyocytes were investigated. The liposome uptake by cardiomyocytes increased dose-dependently and time-dependently. Liposome uptake was strongly influenced by the electrical charge and modified polymer. After 2 h incubation, the uptake of positively charged liposomes was 1.7-fold higher than that of negatively charged one and both higher than that of the neutral one. The presence of PE-PEG2000 distinctly reduced the liposome uptake and the difference between the uptake of charged and neutral liposome. Anoxia increased the uptake of liposome at the first hour (increased 20%), but after 2 h incubation the liposome uptake by hypoxia cellswas less than that of normoxia cells (decreased 18%). Mechanisms involved are also discussed.
Chen
Y
Y
Department of Pharmaceutical Sciences, Shenyang Pharmaceutical University, Liaoning, P.R. China. w.chenyan@263.net
Deng
Y J
YJ
Hao
Y L
YL
Hao
A J
AJ
Zhong
H J
HJ
Wang
X M
XM
D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004563:Electrochemistry; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D008560:Membrane Fluidity; D032383:Myocytes, Cardiac / Q000378:metabolism*; D010743:Phospholipids; D011092:Polyethylene Glycols; D051381:Rats
D008055:Lipids; D008081:Liposomes; D010743:Phospholipids; D011092:Polyethylene Glycols