title: Phylogenetic analysis of several Thermus strains from Rehai of Tengchong, Yunnan, China.
abstract: Several Thermus strains were isolated from 10 hot springs of the Rehai geothermal area in Tengchong, Yunnan province. The diversity of Thermus strains was examined by sequencing the 16S rRNA genes and comparing their sequences. Phylogenetic analysis showed that the 16S rDNA sequences from the Rehai geothermal isolates form four branches in the phylogenetic tree and had greater than 95.9% similarity in the phylogroup. Secondary structure comparison also indicated that the 16S rRNA from the Rehai geothermal isolates have unique secondary structure characteristics in helix 6, helix 9, and helix 10 (reference to Escherichia coli). This research is the first attempt to reveal the diversity of Thermus strains that are distributed in the Rehai geothermal area.
authors.affiliation: Biotechnology Research Center, Faculty of Biological and Chemical Engineering, Kunming University of Science and Technology, Kunming, P.R. China. linlb@public.km.yn.cn
authors.forename: Lianbing
authors.initials: L
authors.lastname: Lin
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Yunlin
authors.initials: Y
authors.lastname: Wei
authors.forename: Chaoyin
authors.initials: C
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Peng
mesh_terms: D001483:Base Sequence; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D014644:Genetic Variation; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013824:Thermus / Q000145:classification* / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S