Expression of PsGRP1, a novel glycine rich protein gene of Pisum sativum, is induced in developing fruit and seed and by ABA in pistil and root.
A novel glycine-rich protein gene, PsGRP1, has been identified in Pisum sativum L. Accumulation of PsGRP1 transcripts was observed in reproductive organs and vegetative tissues. They were localized in endocarp sclerenchyma during fruit development in cells that will lignify. PsGRP1 expression was also detected in senescent pistils and developing seeds and induced by ABA treatment in presenescent pistils. A raise in the expression was also observed in roots after treatment with ABA or mannitol but not under cold stress. A mannitol treatment induced a rise in ABA levels and fluridone treatment counteracted the mannitol induction of PsGRP1 expression. The results suggest a possible role for PsGRP1 in differentiation of the endocarp sclerenchyma and during seed development, pistil senescence and osmotic stress under ABA control.
Urbez
Cristina
C
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia and Consejo Superior de Investigaciones Cientificas, Avenida de los Naranjos s/n, 46022 Valencia, Spain.
Cercós
Manuel
M
Perez-Amador
Miguel A
MA
Carbonell
Juan
J
D000040:Abscisic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D035264:Flowers / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018525:Germination; D005998:Glycine / Q000032:analysis; D008031:Lignin / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment
D010940:Plant Proteins; D008353:Mannitol; D000040:Abscisic Acid; D008031:Lignin; D005998:Glycine