title: Clinicopathologic significance of CD105 expression in squamous cell carcinoma of the hypopharynx.
abstract: BACKGROUND
  Induction of angiogenesis is a key process in tumor growth and metastasis. We studied the CD105 (endoglin) and vascular endothelial growth factor (VEGF) in hypopharyngeal cancer to evaluate their clinicopathologic significance.
  
  
  METHODS
  Seventy-three patients who underwent complete excision of hypopharyngeal cancer were included in this study. Clinicopathologic data were collected retrospectively. Immunohistochemical analysis was performed with antibodies of CD105 and VEGF. Positive-stained microvessels for CD105 were counted on hot spots of tumors. The staining intensity for VEGF was also evaluated.
  
  
  RESULTS
  High expression of CD105 correlated significantly with positive nodal metastasis (p < .001) and TNM stage (p < .01). High expression of VEGF correlated significantly with advanced tumor (T) classification (p < .01). The overall 5-year survival rate was worse in patients with higher CD105 expression (p < .001), advanced TNM stage (p < .01), advanced T classification (p < .01), and positive nodal metastasis (p < .001). Furthermore, Cox regression analysis revealed that CD105 expression in microvessels of tumor tissue, advanced T classification, and nodal status were independent factors for survival.
  
  
  CONCLUSIONS
  Our results suggest that expression of CD105 is a useful predictive prognostic factor in hypopharyngeal cancer.
authors.affiliation: Department of Otolaryngology, Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
authors.forename: Chih-Yen
authors.initials: CY
authors.lastname: Chien
authors.forename: Chih-Ying
authors.initials: CY
authors.lastname: Su
authors.forename: Chung-Feng
authors.initials: CF
authors.lastname: Hwang
authors.forename: Hui-Ching
authors.initials: HC
authors.lastname: Chuang
authors.forename: Yea-Chyang
authors.initials: YC
authors.lastname: Hsiao
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Wu
authors.forename: Chao-Cheng
authors.initials: CC
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology*; D000071063:Endoglin; D005260:Female; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000378:metabolism; D011379:Prognosis; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012189:Retrospective Studies; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A