Gene expression profile analysis in astaxanthin-induced Haematococcus pluvialis using a cDNA microarray.
The unicellular green alga Haematococcus pluvialis (Volvocales) is known for the ketocarotenoid astaxanthin (3, 3'-dihydroxy-beta, beta-carotene-4, 4'-dione) accumulation, which is induced under unfavorable culture conditions. In this work, we used cDNA microarray analysis to screen differentially expressed genes in H. pluvialis under astaxanthin-inductive culture conditions, such as combination of cell exposure to high irradiance and nutrient deprivation. Among the 965 genes in the cDNA array, there are 144 genes exhibiting differential expression (twofold changes) under these conditions. A significant decrease in the expression of photosynthesis-related genes was shown in astaxanthin-accumulating cells (red cells). Defense- or stress-related genes and signal transduction genes were also induced in the red cells. A comparison of microarray and real-time PCR analysis showed good correlation between the differentially expressed genes by the two methods. Our results indicate that the cDNA microarray approach, as employed in this work, can be relied upon and used to monitor gene expression profiles in H. pluvialis. In addition, the genes that were differentially expressed during astaxanthin induction are suitable candidates for further study and can be used as tools for dissecting the molecular mechanism of this unique pigment accumulation process in the green alga H. pluvialis.
Eom
Hyunsuk
H
Department of Biotechnology, Institute of Industrial Biotechnology, Inha University, 253 Yonghyun-Dong, Nam-Gu, 402-751 Incheon, South Korea.
Lee
Choul-Gyun
CG
Jin
EonSeon
E
D020418:Algal Proteins / Q000235:genetics* / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D000460:Chlorophyta / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D020869:Gene Expression Profiling / Q000379:methods; D009584:Nitrogen / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010788:Photosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D024341:Xanthophylls / Q000378:metabolism
D020418:Algal Proteins; D024341:Xanthophylls; D002734:Chlorophyll; D002338:Carotenoids; C005948:astaxanthine; D009584:Nitrogen