[Experimental study of cartilage reconstruction using directed inducing MSCs-alginate complex].
OBJECTIVE
To evaluate the practicability of cartilage reconstruction using directed inducing bone marrow stem cells(MSCs)- alginate complex.


METHODS
The MSCs were seperated by gradient centrifugation on Percoll, then were induced into chon-drogenic differentiation. After 14 days,immunohistochemical techique was applied to detect the expression of collagen type II. Then, the MSCs and calcium alginate were mixed to form a complex and were planted under the skin of nude mice. Histochemical or immuno-histochemical tests were made at 4 weeks and 8 weeks later.


RESULTS
The MSCs showed the positive result of collagen type II. There were chondrcytes and cartilage lacuna at 4 weeks later, and chondrocytes and the collagen secreted by them became more at 8 weeks later. But, the MSCs without directed inducing did not show the character of the chondrocyte.


CONCLUSIONS
The way of using directed inducing MSCs-alginate complex can form cartilage; it is possible to repair the defect of cartilage in clinical practice.
Wang
Guang-Hui
GH
Department of Otolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China.
Wang
Li-Hong
LH
Liang
Chuan-Yu
CY
Li
Wen
W
D000464:Alginates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D020219:Chondrogenesis; D024043:Collagen Type II / Q000096:biosynthesis; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D051379:Mice; D008819:Mice, Nude; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering
D000464:Alginates; D001672:Biocompatible Materials; D024043:Collagen Type II; D006603:Hexuronic Acids; D020723:Glucuronic Acid