title: Continuous wave-based multiphoton excitation fluorescence for capillary electrophoresis.
abstract: It was reported that a novel detection method, continuous wave (CW)-based multiphoton excitation (MPE) fluorescence detection with diode laser (DL), has been firstly proposed for capillary electrophoresis (CE). Special design of end-column detection configuration proved to be superior to on-column type, considering the detection sensitivity. Three different kinds of fluorescent tags that were widely used as molecular label in bio-analysis, such as small-molecule dye, fluorescent protein and nano particle or also referred to as quantum dot (QD), have been evaluated as samples for the constructed detection scheme. Quantitative analyses were also performed using rhodamine species as tests, which revealed dynamic linear range over two orders of magnitude, with detection limit down to zeptomole-level. Simultaneous detection of fluorescent dyestuffs with divergent excitation and emission wavelengths in a broad range showed advantage of this scheme over conventional laser-induced fluorescence (LIF) detection. Further investigations on CW-MPE fluorescence detection with diode laser for capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) separations of fluorescein isothiocyanate (FITC) labeled amino acids indicated good prospect of this detection approach in various micro or nano-column liquid phase separation technologies.
authors.affiliation: Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.forename: Bi-Feng
authors.initials: BF
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Fu
authors.forename: Tao
authors.initials: T
authors.lastname: Xiong
authors.forename: Tiancai
authors.initials: T
authors.lastname: Liu
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhen-Li
authors.initials: ZL
authors.lastname: Huang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.forename: Yuan-Di
authors.initials: YD
authors.lastname: Zhao
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D017785:Photons; D012680:Sensitivity and Specificity