title: Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system.
abstract: We have developed a high-throughput system for generating baculoviruses and testing the expression, solubility, and affinity column purification of encoded proteins. We have used this system to generate baculoviruses for and analyze the expression of 337 proteins from three different herpesviruses (HSV-1, EBV, and CMV) and vaccinia virus. Subsets of these proteins were also tested for expression and solubility in E. coli. Comparisons of the results in the two systems are presented for each virus.
authors.affiliation: Affinium Pharmaceuticals, Toronto, Canada.
authors.forename: Mian
authors.initials: M
authors.lastname: Gao
authors.forename: Nicole
authors.initials: N
authors.lastname: Brufatto
authors.forename: Tricia
authors.initials: T
authors.lastname: Chen
authors.forename: Laura Lea
authors.initials: LL
authors.lastname: Murley
authors.forename: Rosanne
authors.initials: R
authors.lastname: Thalakada
authors.forename: Megan
authors.initials: M
authors.lastname: Domagala
authors.forename: Bryan
authors.initials: B
authors.lastname: Beattie
authors.forename: Daniel
authors.initials: D
authors.lastname: Mamelak
authors.forename: Vicki
authors.initials: V
authors.lastname: Athanasopoulos
authors.forename: David
authors.initials: D
authors.lastname: Johnson
authors.forename: Grant
authors.initials: G
authors.lastname: McFadden
authors.forename: Christian
authors.initials: C
authors.lastname: Burks
authors.forename: Lori
authors.initials: L
authors.lastname: Frappier
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006564:Herpesviridae / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000378:metabolism; D007313:Insecta; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry; D014616:Vaccinia virus / Q000378:metabolism*
chemical_list: D020543:Proteome; D011994:Recombinant Proteins