title: Influences of bracket bonding on mutans streptococcus in plaque detected by real time fluorescence-quantitative polymerase chain reaction.
abstract: BACKGROUND
  Enamel demineralization occurs frequently during orthodontic treatment. In this study, we evaluated the changes of the density of mutans streptococcus (MS) in plaque after bracket bonding and using fluoride adhesive on maxillary incisors by real time fluorescence-quantitative polymerase chain reaction (RT-FQ PCR).
  
  
  METHODS
  The study was designed as a self-paired test. Brackets were bonded with fluoride adhesive on the left side, while non-fluoride adhesive on the right side for each patient. Plaque samples were taken from the surfaces around the brackets of four maxillary incisors before brackets bonding and after the bonding 4 weeks later. The amount of MS was measured by RT-FQ PCR. The data obtained were analyzed statistically using the SPSS 11.5 version and the alpha level was set at 0.05 (2-tailed).
  
  
  RESULTS
  The amount of MS in plaque increased significantly after bracket bonding (P < 0.01), whereas no significant differences were observed among four maxillary incisors both before and after brackets bonding (P > 0.05), and among the incisors using and not using fluoride adhesive (P > 0.05).
  
  
  CONCLUSIONS
  The increase of the density of MS in plaque after bracket bonding is one of the etiological factors for enamel demineralization in orthodontic patients. The result of this study did not support what we observed clinically that the incidence of enamel demineralization for lateral incisors was higher than that for central incisors. Using fluoride adhesive for bonding did not affect the amount of MS in plaque in our study. Further study is needed.
authors.affiliation: Department of Stomatology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510640, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ai
authors.forename: Hong-fei
authors.initials: HF
authors.lastname: Lu
authors.forename: Huan-you
authors.initials: HY
authors.lastname: Liang
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Ruo-lan
authors.initials: RL
authors.lastname: Li
authors.forename: Guo-ping
authors.initials: GP
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Xi
mesh_terms: D000269:Adhesives; D000293:Adolescent; D001840:Dental Bonding; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D005453:Fluorescence; D005459:Fluorides / Q000008:administration & dosage; D006801:Humans; D008297:Male; D016910:Orthodontic Brackets; D016133:Polymerase Chain Reaction / Q000379:methods*; D013295:Streptococcus mutans / Q000235:genetics / Q000302:isolation & purification*; D017001:Tooth Demineralization
chemical_list: D000269:Adhesives; D005459:Fluorides