The octamer binding transcription factor Oct-1 is a stress sensor.
The POU-domain transcription factor Oct-1 is widely expressed in adult tissues and has been proposed to regulate a large group of target genes. Microarray expression profiling was used to evaluate gene expression changes in Oct-1-deficient mouse fibroblasts. A number of genes associated with cellular stress exhibited altered expression. Consistent with this finding, Oct-1-deficient fibroblasts were hypersensitive to gamma radiation, doxorubicin, and hydrogen peroxide and harbored elevated reactive oxygen species. Expression profiling identified a second group of genes dysregulated in Oct-1-deficient fibroblasts following irradiation, including many associated with oxidative and metabolic stress. A number of these genes contain octamer sequences in their immediate 5' regulatory regions, some of which are conserved in human. These results indicate that Oct-1 modulates the activity of genes important for the cellular response to stress.
Tantin
Dean
D
Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139-4307, USA.
Schild-Poulter
Caroline
C
Wang
Victoria
V
Haché
Robert J G
RJ
Sharp
Phillip A
PA
D016475:3T3 Cells; D000818:Animals; D004317:Doxorubicin; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D006861:Hydrogen Peroxide; D051379:Mice; D050811:Octamer Transcription Factor-1 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D018384:Oxidative Stress; D020133:Reverse Transcriptase Polymerase Chain Reaction
D050811:Octamer Transcription Factor-1; D004317:Doxorubicin; D006861:Hydrogen Peroxide