title: Isolation of copper-tolerant mutants of sake yeast with defective peptide uptake.
abstract: Mutants of Saccharomyces cerevisiae that have a decreased peptide uptake ability were isolated from sake yeast. A copper medium containing copper sulfate, histidylleucine and sodium lactate as a carbon source was used for the isolation. One mutant showed a decreased peptide uptake ability due to PTR2 transcriptional repression, but for other mutants, the decrease was not due to the transcriptional repression. The peptide concentration in sake brewed with these mutants increased to about 1.5-fold that of sake brewed with the parental strain. The angiotensin I-converting enzyme (ACE) inhibitory activity of sake brewed with the mutant was about 3.6-fold that of sake brewed with the parental strain.
authors.affiliation: General Research Laboratory of Kiku-Masamune Sake Brewing Co., Ltd., 1-8-6 Uozaki-nishimachi, Higashinada-ku, Kobe 658-0026, Japan. ta-yamada@kikumasamune.co.jp
authors.forename: Tasuku
authors.initials: T
authors.lastname: Yamada
authors.forename: Keiji
authors.initials: K
authors.lastname: Furukawa
authors.forename: Shodo
authors.initials: S
authors.lastname: Hara
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Mizoguchi
mesh_terms: D000434:Alcoholic Beverages / Q000382:microbiology; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D001483:Base Sequence; D003300:Copper / Q000378:metabolism / Q000494:pharmacology*; D004351:Drug Resistance / Q000235:genetics; D005285:Fermentation; D018398:Homeodomain Proteins / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010455:Peptides / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000187:drug effects; D021381:Protein Transport / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000235:genetics
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; C090457:CUP9 protein, S cerevisiae; D018398:Homeodomain Proteins; D026901:Membrane Transport Proteins; C093495:PTR2 protein, S cerevisiae; D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D003300:Copper; C066066:UBR1 protein, S cerevisiae; D044767:Ubiquitin-Protein Ligases; D007703:Peptidyl-Dipeptidase A