title: Fast high performance liquid chromatography and ultraviolet-visible quantification of principal phenolic antioxidants in fresh rosemary.
abstract: An improved HPLC method is reported for the determination of rosemary's principal phenolic antioxidants, rosmarinic and carnosic acids, providing a fast and simultaneous determination for both of them by using a solid phase column. The analysis was performed with fresh methanolic extractions of Rosmarinus officinalis. To quantify the amount of antioxidants in a fast and reproducible way by means of UV-vis absorption measurements, a spectrophotometric multi-wavelength calibration curve was constructed based on the antioxidant contents obtained with the recently developed HPLC method. This UV-vis methodology can be extended to the determination of other compounds and herbs if the restrictions mentioned in the text are respected.
authors.affiliation: Fundación Chile, Area Agroindustria, Parque Rabat 6165 Vitacura, Santiago, Chile.
authors.forename: N
authors.initials: N
authors.lastname: Troncoso
authors.forename: H
authors.initials: H
authors.lastname: Sierra
authors.forename: L
authors.initials: L
authors.lastname: Carvajal
authors.forename: P
authors.initials: P
authors.lastname: Delpiano
authors.forename: G
authors.initials: G
authors.lastname: Günther
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D010945:Plants, Edible / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000975:Antioxidants; D010636:Phenols