title: Fetal cells in a transcervical cell sample collected at 5 weeks of gestation.
abstract: Transcervical cell (TCC) sampling is being investigated as a promising method for obtaining fetal cells for prenatal genetic diagnosis. The present case report describes the identification of fetal cells by both fluorescent in situ hybridisation (FISH) and quantitative fluorescent polymerase chain reaction (QF-PCR) analyses in a TCC sample collected by intrauterine lavage at 5 + 0 weeks. This finding underscores the possible relevance of TCC sampling for extremely early prenatal genetic diagnosis.
authors.affiliation: Department of Gynecology, Perinatology and Human Reproduction, University of Florence, Careggi University Hospital, Florence, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Cioni
authors.forename: Cecilia
authors.initials: C
authors.lastname: Bussani
authors.forename: Sandra
authors.initials: S
authors.lastname: Bucciantini
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Scarselli
mesh_terms: D005260:Female; D005333:Fetus / Q000166:cytology*; D005453:Fluorescence; D005820:Genetic Testing; D005865:Gestational Age; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D010920:Placenta / Q000166:cytology; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D019849:Sex Determination Processes; D007507:Therapeutic Irrigation; D014599:Uterus / Q000166:cytology*