title: Cloning and characterization of the canine receptor for advanced glycation end products.
abstract: Metastasis is one of the major problems when dealing with malignant neoplasias. Accordingly, the finding of molecular targets, which can be addressed to reduce tumour metastasising, will have significant impact on the development of new therapeutic approaches. Recently, the receptor for advanced glycation end products (RAGE)-high mobility group B1 (HMGB1) protein complex has been shown to have significant influence on invasiveness, growth and motility of tumour cells, which are essential characteristics required for metastatic behaviour. A set of in vitro and in vivo approaches showed that blocking of this complex resulted in drastic suppression of tumour cell growth. Due to the similarities of human and canine cancer the dog has joined the common rodent animal model for therapeutic and preclinical studies. However, complete characterisation of the protein complex is a precondition to a therapeutic approach based on the blocking of the RAGE-HMGB1 complex to spontaneously occurring tumours in dogs. We recently characterised the canine HMGB1 gene and protein completely. Here we present the complete characterisation of the canine RAGE gene including its 1384 bp mRNA, the 1215 bp protein coding sequence, the 2835 bp genomic structure, chromosomal localisation, gene expression pattern, and its 404 amino acid protein. Furthermore we compared the CDS of six different canine breeds and screened them for single nucleotide polymorphisms.
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine, Bischofsholer Damm 15, D-30173 Hannover, Germany. escobar@uni-bremen.de
authors.forename: Hugo
authors.initials: H
authors.lastname: Murua Escobar
authors.forename: Jan T
authors.initials: JT
authors.lastname: Soller
authors.forename: Katharina A
authors.initials: KA
authors.lastname: Sterenczak
authors.forename: Jan D
authors.initials: JD
authors.lastname: Sperveslage
authors.forename: Claudia
authors.initials: C
authors.lastname: Schlueter
authors.forename: Birgit
authors.initials: B
authors.lastname: Burchardt
authors.forename: Nina
authors.initials: N
authors.lastname: Eberle
authors.forename: Melanie
authors.initials: M
authors.lastname: Fork
authors.forename: Rolf
authors.initials: R
authors.lastname: Nimzyk
authors.forename: Susanne
authors.initials: S
authors.lastname: Winkler
authors.forename: Ingo
authors.initials: I
authors.lastname: Nolte
authors.forename: Jörn
authors.initials: J
authors.lastname: Bullerdiek
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004285:Dogs; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic