title: Regulation of magnesium-inhibited cation current by actin cytoskeleton rearrangement.
abstract: Magnesium-inhibited, non-selective cation current (I(MIC)) is activated by depletion of intracellular Mg(2+) and ATP. I(MIC) transports various divalent cations including Mg(2+) and Ca(2+), and is involved in cell viability. We investigated the effect of actin dynamics on I(MIC). Formation of a stable cortical actin network by calyculin A inhibited the activation of I(MIC), while the actin depolymerizing reagent, cytochalasin D, reversed the inhibition. Induction of a dense cortical actin layer by transfecting the constitutively active form of RhoA also inhibited the activation of I(MIC). These results suggest that the activation of I(MIC) may be dynamically regulated by actin cytoskeleton rearrangement.
authors.affiliation: Department of Physiology, Sungkyunkwan University School of Medicine, Suwon 440-746, South Korea.
authors.forename: Soon Youn
authors.initials: SY
authors.lastname: Jeong
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Shin
authors.forename: Han-Seop
authors.initials: HS
authors.lastname: Kim
authors.forename: Chang-Dae
authors.initials: CD
authors.lastname: Bae
authors.forename: Dae-Yong
authors.initials: DY
authors.lastname: Uhm
authors.forename: Myung-Kyu
authors.initials: MK
authors.lastname: Park
authors.forename: Sungkwon
authors.initials: S
authors.lastname: Chung
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure*; D002412:Cations; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D019169:Jurkat Cells; D008274:Magnesium / Q000494:pharmacology*
chemical_list: D002412:Cations; D007473:Ion Channels; D008274:Magnesium