Self-assembly of actin scaffolds at ponticulin-containing supported phospholipid bilayers.
Phospholipid vesicles containing ponticulin have been used to form solid supported and tethered bilayer lipid membranes. The ponticulin serves as both a nucleation site for actin polymerization as well as a binding site for F-actin. Studies of F-actin binding to such bilayers have demonstrated the formation of an in vitro actin scaffold. The dissociation constant for the binding of F-actin filaments to a ponticulin-containing tethered bilayer was found to be 11 +/- 5 nM, indicative of high affinity binding.
Johnson
Benjamin R G
BR
Bushby
Richard J
RJ
Colyer
John
J
Evans
Stephen D
SD
D000199:Actins / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D001703:Biophysics / Q000379:methods*; D002352:Carrier Proteins / Q000737:chemistry*; D004023:Dictyostelium / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D008840:Microfilament Proteins / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D011485:Protein Binding
D000199:Actins; D002352:Carrier Proteins; C034037:F-actin-binding proteins; D008051:Lipid Bilayers; D008055:Lipids; D008840:Microfilament Proteins; D010713:Phosphatidylcholines; D010743:Phospholipids