[The role of apoptosis in ishemic damage to the myocardium].
36 specimens of the myocardium were obtained during necropsy of patients who had died of acute cardiac failure. Bcl-2, caspase 3 and p53 were studied immunohistochemically. TUNEL method was used to study apoptic DNA fragmentation in cardiomyocytes (Cms). The results suggest that apoptosis contributes to the development of cardiomyocyte injury in ishemia. Apoptotic death of Cms may lead to electric instabilly of the myocardium in early ishemia and cause sudden cardiac death. Apoptosis of the Cms may precede extension of the necrotic area. Further studies of apoptotic death of Cms in ishemic heart disease may help to renew therapeutic strategies aimed at regulation of apoptosis.
Rybakova
M G
MG
Kuznetsova
I A
IA
D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases / Q000032:analysis / Q000378:metabolism; D053938:DNA Fragmentation; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000473:pathology*; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000378:metabolism
D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases