title: A live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine induces a partial cross-protection against caprine herpesvirus 1 infection in goats.
abstract: Taking into account the close antigenic relationship between bovine herpesvirus 1 (BoHV-1) and caprine herpesvirus 1 (CpHV-1), a live attenuated glycoprotein E (gE) negative BoHV-1 vaccine was assessed in goats with the aim to protect against CpHV-1 infection. Vaccine safety was evaluated by intranasal inoculation of two groups of goats with either a gE-negative BoHV-1 vaccine or a virulent BoHV-1. The length of viral excretion and the peak viral titre were reduced with the gE-negative vaccine. To assess the efficacy, two goats were inoculated intranasally twice 2 weeks apart with a gE-negative BoHV-1 vaccine. Four weeks later, immunised and control goats were challenged with CpHV-1. A 2 log(10) reduction in the peak viral titre was observed and the challenge virus excretion lasted 2 days more in immunised than in control goats. These data indicate the safety and the partial efficacy of a live attenuated gE-negative BoHV-1 vaccine intranasally administrated in goats.
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine, University of Bari, 70010 Valenzano, Italy. julien.thiry@ulg.ac.b
authors.forename: Julien
authors.initials: J
authors.lastname: Thiry
authors.forename: Maria
authors.initials: M
authors.lastname: Tempesta
authors.forename: Michele
authors.initials: M
authors.lastname: Camero
authors.forename: Elvira
authors.initials: E
authors.lastname: Tarsitano
authors.forename: Anna Lucia
authors.initials: AL
authors.lastname: Bellacicco
authors.forename: Etienne
authors.initials: E
authors.lastname: Thiry
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D015511:Goat Diseases / Q000517:prevention & control*; D006041:Goats; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D022283:Herpesvirus Vaccines; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011897:Random Allocation; D012449:Safety; D013997:Time Factors; D016896:Treatment Outcome; D014613:Vaccines, Attenuated; D018140:Varicellovirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000276:immunology*; D014764:Viral Proteins
chemical_list: D004279:DNA, Viral; D022283:Herpesvirus Vaccines; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins