Mercury influences rat liver tyrosine aminotransferase activity and induction by dexamethasone.
The effects of mercury (Hg) on basal and dexamethasone-induced tyrosine aminotransferase (TAT) activity in rat liver were studied. Comparison of TAT activity after in vitro and in vivo mercury application revealed the influence of the metal only when applied in vivo, suggesting that the effects are expressed at the level of TAT gene transcription. Intraperitoneal administration of mercury at 1, 2 or 3 mg Hg kg(-1) b.w. 4 h before decapitation was shown to stimulate the basal activity of TAT. The most prominent increase was observed 4 h after the metal administration. When applied at 1 and 2 mg Hg kg(-1) b.w. mercury was also shown to reduce partially the extent of the enzyme induction by dexamethasone, which was injected intraperitoneally at 5 mg kg(-1) b.w. 5 h before death. The highest dose of mercury (3 mg Hg kg(-1) b.w.) almost completely abolished the dexamethasone effect. The finding that mercury increases basal activity of the enzyme while decreasing its induction by dexamethasone suggests that stimulatory effects of this metal on TAT activity are probably mediated by factors other than glucocorticoids.
Dundjerski
Jadranka
J
Department of Biochemistry, Institute for Biological Research, Belgrade, Serbia and Montenegro. jadund@ibiss.bg.ac.yu
Brkljacić
Jelena
J
Elaković
Ivana
I
Manitasević
Sanja
S
Matić
Gordana
G
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008628:Mercury / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000187:drug effects; D013054:Spectrophotometry, Atomic; D014444:Tyrosine Transaminase / Q000096:biosynthesis / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D014444:Tyrosine Transaminase; D008628:Mercury