Infection of Rosellinia necatrix with purified viral particles of a member of Partitiviridae (RnPV1-W8).
Isolate W8 of the white root rot fungus, Rosellinia necatrix, harbors three dsRNA segments, L1-, L2- and M-dsRNAs, and showed an irregular colony margin, slow growth, and moderate virulence. The M-dsRNA was previously shown to be the genome of a partitivirus, RnPV1-W8. Here a transfection protocol was developed for RnPV1-W8. Protoplasts of two virus-free isolates of R. necatrix were inoculated with purified viral particles using a polyethylene glycol-mediated method. Virus infection was confirmed by electrophoresis and Northern analysis. RnPV1-W8 introduced into the new host isolates was transmissible via hyphal anastomosis. However, the infection had no effect on the morphology and virulence of infected isolates of R. necatrix. This is the first report on the transfection of a partitivirus for R. necatrix.
Sasaki
A
A
Department of Plant Protection, National Institute of Fruit Tree Science, National Agricultural Research Organization, Tsukuba, Japan. sasaattu@affrc.go.jp
Kanematsu
S
S
Onoue
M
M
Oyama
Y
Y
Yoshida
K
K
D001203:Ascomycota / Q000235:genetics / Q000472:pathogenicity / Q000821:virology; D025301:Hyphae / Q000378:metabolism / Q000821:virology; D063245:Plant Development; D010935:Plant Diseases / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D010944:Plants / Q000382:microbiology; D011523:Protoplasts / Q000821:virology; D031989:Pyrus / Q000382:microbiology*; D012328:RNA Viruses / Q000235:genetics* / Q000502:physiology; D014162:Transfection; D014779:Virus Replication