Formation of insoluble magnesium phosphates during growth of the archaea Halorubrum distributum and Halobacterium salinarium and the bacterium Brevibacterium antiquum.
Stationary phase cells of the halophilic archaea Halobacterium salinarium and Halorubrum distributum, growing at 3-4 M NaCl, and of the halotolerant bacterium Brevibacterium antiquum, growing with and without 2.6 NaCl, took up approximately 90% of the phosphate from the culture media containing 2.3 and 11.5 mM phosphate. The uptake was blocked by the uncoupler FCCP. In B. antiquum, EDTA inhibited the phosphate uptake. The content of polyphosphates in the cells was significantly lower than the content of orthophosphate. At a high phosphate concentration, up to 80% of the phosphate taken up from the culture medium was accumulated as Mg(2)PO(4)OH x 4H(2)O in H. salinarium and H. distributum and as NH(4)MgPO(4) x 6H(2)O in B. antiquum. Consolidation of the cytoplasm and enlargement of the nucleoid zone were observed in the cells during phosphate accumulation. At phosphate surplus, part of the H. salinarium and H. distributum cell population was lysed. The cells of B. antiquum were not lysed and phosphate crystals were observed in the cytoplasm.
Smirnov
Aleksey
A
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
Suzina
Natalia
N
Chudinova
Natalia
N
Kulakovskaya
Tatiana
T
Kulaev
Igor
I
D001105:Archaea / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D001951:Brevibacterium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000633:toxicity; D003593:Cytoplasm / Q000378:metabolism; D017616:Magnesium Compounds / Q000378:metabolism*; D008854:Microscopy, Electron; D010710:Phosphates / Q000302:isolation & purification / Q000378:metabolism / Q000493:pharmacokinetics*; D012965:Sodium Chloride / Q000378:metabolism; D013054:Spectrophotometry, Atomic; D013997:Time Factors; D014475:Uncoupling Agents / Q000633:toxicity
D017616:Magnesium Compounds; D010710:Phosphates; D014475:Uncoupling Agents; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D012965:Sodium Chloride; C030781:magnesium phosphate