Morphology of elastase-induced cerebral aneurysm model in rabbit and rapid prototyping of elastomeric transparent replicas.
In this work, we describe a methodology to fabricate transparent elastomeric vascular replicas using rapid prototyping techniques. First, the three-dimensional morphology of an elastase-induced aneurysm model in rabbit is acquired. The morphology is reconstructed from in vivo rotational angiography and it is compared with three-dimensional reconstructions obtained by computerized tomography and magnetic resonance imaging of an intraluminal arterial cast that was obtained from the same animal at sacrifice. Results show that resolution of the imaging modality strongly influences the level of detail, such as small side branches, in the final reconstruction. We developed an average morphology model for elastase-induced aneurysms in rabbits including the surrounding vasculature and describe a method for rapid prototyping of vascular models from the three-dimensional morphology. Our replicas can be manufactured in a short period of time and the final product is optically clear. In addition, the elasticity of the models can be controlled to represent arterial elasticity, which makes them ideal for optical investigations of detailed flow dynamics using measurement tools such as particle image velocimetry.
Seong
Jaehoon
J
Department of Biomedical Engineering, University of Miami, Coral Gables, FL 33146, USA.
Sadasivan
Chander
C
Onizuka
Masanari
M
Gounis
Matthew J
MJ
Christian
Fletcher
F
Miskolczi
Laszlo
L
Wakhloo
Ajay K
AK
Lieber
Baruch B
BB
D000818:Animals; D002533:Cerebral Angiography; D002536:Cerebral Arteries / Q000473:pathology*; D004548:Elasticity; D020724:Elastomers; D021621:Imaging, Three-Dimensional; D002532:Intracranial Aneurysm / Q000000981:diagnostic imaging / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D008953:Models, Anatomic; D023421:Models, Animal; D010196:Pancreatic Elastase; D019047:Phantoms, Imaging; D011108:Polymers; D011817:Rabbits; D012828:Silicones; D014057:Tomography, X-Ray Computed
D020724:Elastomers; D011108:Polymers; D012828:Silicones; C036925:elastomeric polymer; D010196:Pancreatic Elastase