title: Prevention of bone loss by phloridzin, an apple polyphenol, in ovariectomized rats under inflammation conditions.
abstract: Aging and sex hormones related changes lead to inflammatory and oxidant conditions, which are involved in the pathogenesis of osteoporosis. Recent studies have suggested that polyphenols may exert a protective effect in such conditions. We assessed the effect of phloridzin (Phlo), a flavonoid exclusively found in apple, on bone metabolism in ovariectomized (OVX) or sham-operated (SH) rats with and without inflammation. Six-month-old Wistar rats were allocated to two equal groups that received either a control diet or a diet supplemented with 0.25% Phlo for 80 days. Three weeks before necropsy, inflammation was induced by subcutaneous injection of talc in 10 animals of each group. At necropsy, ovariectomy decreased both total (T-BMD) and metaphyseal (M-BMD) femoral bone mineral density (P < 0.01). Inflammation conditions, checked by an increase in the spleen weight and alpha1-acid glycoprotein concentration in OVX rats, exacerbated the decrease in T-BMD (g/cm2) (as well as M-BMD) observed in castrated animals (P < 0.05). Daily Phlo intake prevented ovariectomy-induced bone loss in conditions of inflammation as shown by T-BMD and M-BMD (P < 0.05). At the diaphyseal site, BMD was improved by Phlo in OVX rats with or without inflammation (P < 0.05). These results could be explained by changes in bone remodeling as the increased urinary deoxypyridinoline excretion in OVX and OVXinf animals was prevented by the polyphenol-rich diet (P < 0.001), while plasma osteocalcin concentration was similar in all experimental groups. In conclusion, Phlo consumption may provide protection against ovariectomy-induced osteopenia under inflammation conditions by improving inflammation markers and bone resorption.
authors.affiliation: Unité des Maladies Métaboliques et Micronutriments, INRA Theix, 63122, Saint Genès-Champanelle, France.
authors.forename: C
authors.initials: C
authors.lastname: Puel
authors.forename: A
authors.initials: A
authors.lastname: Quintin
authors.forename: J
authors.initials: J
authors.lastname: Mathey
authors.forename: C
authors.initials: C
authors.lastname: Obled
authors.forename: M J
authors.initials: MJ
authors.lastname: Davicco
authors.forename: P
authors.initials: P
authors.lastname: Lebecque
authors.forename: S
authors.initials: S
authors.lastname: Kati-Coulibaly
authors.forename: M N
authors.initials: MN
authors.lastname: Horcajada
authors.forename: V
authors.initials: V
authors.lastname: Coxam
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects / Q000502:physiology; D001851:Bone Diseases, Metabolic / Q000150:complications / Q000503:physiopathology / Q000517:prevention & control*; D001862:Bone Resorption / Q000188:drug therapy* / Q000503:physiopathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D007249:Inflammation / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D027845:Malus / Q000737:chemistry*; D009929:Organ Size / Q000187:drug effects; D015675:Osteocalcin / Q000097:blood; D010052:Ovariectomy; D010695:Phlorhizin / Q000627:therapeutic use*; D010936:Plant Extracts / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D016474:Weight-Bearing
chemical_list: D010936:Plant Extracts; D015675:Osteocalcin; D010695:Phlorhizin