Interaction effect in bladder cancer between N-acetyltransferase 2 genotype and alcohol drinking.
Cigarette smoking, aromatic amines and ionizing radiation are known carcinogens of bladder cancer. NAT2 genotype might play a role in bladder cancer carcinogenesis. This hospital-based, case-control study was conducted in Kaohsiung, Taiwan, which neighbors the high incidence region of bladder cancer in the black-foot disease area. A total of 103 cases with diagnosed bladder cancer were collected. For each case, 1 control was selected from the same hospital. A structured questionnaire was applied for all cases and controls. DNA was extracted from peripheral blood cell. The ASO-PCR/RFLP technique was used to determine the NAT2 genotype. For bladder cancer, the significantly excessive risks were observed in regular drinkers (OR = 2.74, 95% CI = 1.28-5.87) and residents of the black-foot disease endemic area (OR = 7.53, 95% CI = 2.16-26.33), and interaction of regular drinking and slow type of NAT2 (OR = 18.04, 95% CI = 2.28-142.80). We suggested that NAT2 genotype might play a role of effect modifier in bladder cancer carcinogenesis.
Lu
Chih-Ming
CM
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Chung
Meng-Chu
MC
Huang
Chung-Hsiung
CH
Ko
Ying-Chin
YC
D000368:Aged; D000428:Alcohol Drinking / Q000009:adverse effects* / Q000097:blood / Q000235:genetics; D001191:Arylamine N-Acetyltransferase / Q000097:blood / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000209:etiology / Q000235:genetics
D004273:DNA, Neoplasm; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human