Quantifying protein-protein interactions in the ubiquitin pathway by surface plasmon resonance.
The commercial availability of instruments, such as Biacore, that are capable of monitoring surface plasmon resonance (SPR) has greatly simplified the quantification of protein-protein interactions. Already, this technique has been used for some studies of the ubiquitin-proteasome system. Here we discuss some of the problems and pitfalls that researchers should be aware of when using SPR analyses for studies of the ubiquitin-proteasome system.
Hartmann-Petersen
Rasmus
R
Department of Biochemistry, Institute for Molecular Biology and Physiology, Copenhagen University, Copenhagen, Denmark.
Gordon
Colin
C
D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods*; D025801:Ubiquitin / Q000378:metabolism*
D011506:Proteins; D025801:Ubiquitin