Enhanced production of the polysaccharide arabinogalactan using immobilized cultures of Tinospora cordifolia by elicitation and in situ adsorption.
Immobilized callus cultures of Tinospora cordifolia (Willd) Miers ex Hooks and Thoms were investigated to find out the combined effect of elicitation, cell permeabilization with chitosan and in situ product recovery by polymeric neutral resin-like Diaion HP 20. In this study, callus cultures of T. cordifolia were immobilized using sodium alginate and calcium chloride and the beads were cultured in Murashige and Skoog's basal medium along with benzyl adenine (BA), 2,4-dichlorophenoxy acetic acid (2,4-D) and 3% sucrose. The immobilized cultures, when subjected to elicitation and cell permeabilization with chitosan and in situ removal of the secondary metabolites by addition of resin, showed a 10-fold increase in production of arabinogalactan (0.490% dry weight) as compared to respective controls devoid of resin and chitosan. This indicates that in situ adsorption may have reduced the feedback inhibition caused by accumulation of secondary metabolites in the media, while the dual effect of elicitation and cell permeabilization by chitosan may have released the intracellular (secreted) berberine and the polysaccharide arabinogalactan, respectively.
Roja
G
G
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 4000 85, India. groja@magnum.barc.ernet.in
Bhangale
A S
AS
Juvekar
A R
AR
Eapen
S
S
D'Souza
S F
SF
D000327:Adsorption; D001599:Berberine / Q000302:isolation & purification / Q000378:metabolism; D001709:Biotechnology; D018914:Cells, Immobilized; D005685:Galactans / Q000096:biosynthesis* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007475:Ion Exchange Resins; D007700:Kinetics; D011137:Polystyrenes; D031610:Tinospora / Q000378:metabolism*
D005685:Galactans; D007475:Ion Exchange Resins; D011137:Polystyrenes; D001599:Berberine; C065649:Diaion HP 20; C005653:arabinogalactan