title: [Study on the extraction recovery of ginsenoside Re in plasma by different solid-phase cartridges].
abstract: OBJECTIVE
  To optimize the solid-phase extraction method by comparison of the extraction recovery of ginsenoside Re plasma samples.
  
  
  METHODS
  After extracted by different solid-phase cartridges with water, acetonitrile, and different content methanol elution, the plasma samples were analyzed on an Zorbax SB-C18 column with acetonitrile-water gradient elution. From the recovery achieved, the best solid phase cartridge was found.
  
  
  RESULTS
  This method consists of using 40% methanol as the wash solvent, and 80% methanol for the elution. Among the three kinds of solid-phase being tested, Waters Oasis HLB cartridge was found to be the best one.
  
  
  CONCLUSIONS
  The average extraction recovery of the Waters Oasis HLB cartridges was between 103%-113%, it can be used in the analysis of ginsenoside Re in plasma samples.
authors.affiliation: China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Xiang-Jun
authors.initials: XJ
authors.lastname: Mao
authors.forename: Chen-Feng
authors.initials: CF
authors.lastname: Zhang
authors.forename: Di
authors.initials: D
authors.lastname: Sun
authors.forename: Long-Sheng
authors.initials: LS
authors.lastname: Sheng
authors.forename: Guang-Ji
authors.initials: GJ
authors.lastname: Wang
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D036145:Ginsenosides; C049864:ginsenoside Re