title: Expression patterns among interferon regulatory factor-1, human X-box binding protein-1, nuclear factor kappa B, nucleophosmin, estrogen receptor-alpha and progesterone receptor proteins in breast cancer tissue microarrays.
abstract: Interferon regulatory factor-1 (IRF-1), human X-box binding protein-1 (hXBP-1), nuclear factor kappa B p65 (NFkappaB p65) and nucleophosmin (NPM) have been implicated in a signaling network of endocrine responsiveness. Expression of these proteins was measured by immunohistochemistry in tissue microarrays of 54 breast tumors. Correlations between each protein and established prognostic markers were assessed by Spearman's rank order correlation coefficient and partial correlation coefficient analyses. Moderate/strong staining is seen for hXBP-1 (79% of tumors) and NFkappaB p65 (57%). NPM exhibits nuclear staining (95%); IRF-1 exhibits both cytosolic (IRF-1c; 90%) and nuclear staining (IRF-1n; 51%). IRF-1c is associated with age (p=0.034); IRF-1n and PgR expression are correlated (p=0.014). NFkappaB p65 shows a borderline association with S phase (p=0.062). Coexpression of IRF-1c and hXBP1 (p=0.001), IRF-1c and NFkappaB (p=0.002), and hXBP-1 and NFkappaB (p=0.018) is observed. An inverse correlation exists between IRF-1n and NFkappaB (p=0.034). All four proteins are detected in breast tumors and their expression patterns support their role(s) in a key signaling network.
authors.affiliation: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA.
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Zhu
authors.forename: Baljit
authors.initials: B
authors.lastname: Singh
authors.forename: Stephen
authors.initials: S
authors.lastname: Hewitt
authors.forename: Aiyi
authors.initials: A
authors.lastname: Liu
authors.forename: Bianca
authors.initials: B
authors.lastname: Gomez
authors.forename: Antai
authors.initials: A
authors.lastname: Wang
authors.forename: Robert
authors.initials: R
authors.lastname: Clarke
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D047628:Estrogen Receptor alpha / Q000096:biosynthesis; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D050856:Interferon Regulatory Factor-1 / Q000096:biosynthesis; D008875:Middle Aged; D016328:NF-kappa B / Q000096:biosynthesis; D009687:Nuclear Proteins / Q000096:biosynthesis; D000090243:Nucleophosmin; D020411:Oligonucleotide Array Sequence Analysis; D011980:Receptors, Progesterone / Q000096:biosynthesis; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; D047628:Estrogen Receptor alpha; C494257:IRF1 protein, human; D050856:Interferon Regulatory Factor-1; D016328:NF-kappa B; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D011980:Receptors, Progesterone; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D000090243:Nucleophosmin