[Detection of glucocorticoid receptor-alpha mRNA expression using FQ-RT-PCR in nasal polyp].
OBJECTIVE
To detect the expression of the glucocorticoid receptor-alpha (GR-alpha) mRNA in nasal polyp and normal nasal mucosa and investigate the significance of GR-alpha in progression of nasal polyp.


METHODS
The expression of GR-alpha mRNA in 36 samples of nasal polyp and 31 samples of normal nasal mucosa was examined by using fluorescent quantitative PCR(FQ RT-PCR).


RESULTS
The GR-alpha mRNA levels in normal nasal mucosa [(135.4 +/- 5.2) x 10(10) copy/g] were significantly higher than those in nasal polyp [(25.5 +/- 5.0) x 10(10) copy/g] (P < 0.01).


CONCLUSIONS
The low expression of GR-alpha mRNA in nasal polyp is related to the progression of nasal polyp.
Li
Peng
P
Department of Otolaryngology-Head and Neck Surgery, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, China. lp76@tom.com
Li
Yuan
Y
Zhang
Xiaowen
X
Zhang
Gehua
G
Ye
Jin
J
Sun
Yanan
Y
Hu
Bin
B
D000328:Adult; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D009298:Nasal Polyps / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; C411470:glucocorticoid receptor alpha