Cell volume increase in murine MC3T3-E1 pre-osteoblasts attaching onto biocompatible tantalum observed by magnetic AC mode atomic force microscopy.
Magnetic AC mode (MACmode) atomic force microscopy (AFM) was used to study murine (mouse) MC3T3-E1 preosteoblastic cells attached to biocompatible tantalum substrates. Cell volumes of attached cells derived from AFM images were compared to volumes of detached cells in suspension measured by the Coulter sizing technique. An increase of approximately 50% in cell volume was observed when the cells attached to planar tantalum substrates and developed a flattened structure including lamellipodia. We address thoroughly the issues general to the AFM determination of absolute cell volumes, and compare our magnetic AC mode AFM measurements to hitherto reported cell volume determinations by contact mode AFM.
Andersen
L Klembt
LK
Interdisciplinary Nanoscience Center (iNANO), Department of Physics and Astronomy, University of Aarhus, DK-8000 Aarhus, Denmark. klembt@phys.au.dk
Contera
S Antoranz
SA
Justesen
J
J
Duch
M
M
Hansen
O
O
Chevallier
J
J
Foss
M
M
Pedersen
F S
FS
Besenbacher
F
F
D000818:Animals; D001672:Biocompatible Materials; D048429:Cell Size; D002478:Cells, Cultured; D008280:Magnetics; D051379:Mice; D018625:Microscopy, Atomic Force / Q000379:methods*; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure*; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D013635:Tantalum / Q000378:metabolism*; D016707:Tissue Fixation
D001672:Biocompatible Materials; D013635:Tantalum