Involvement of GATA-4/-5 transcription factors in ovarian carcinogenesis.
To clarify the role of GATA transcription factors in ovarian carcinogenesis, we analyzed the expression and methylation states of GATA-4/-5/-6 in eight human ovarian cancer cell lines. GATA-4/-5 were methylated in three and two cell lines without their expression, respectively. Methylation of GATA-4/-5 was also detected in nine and five of 15 primary ovarian cancers, respectively. GATA-6 was not methylated in any cases. We transiently over-expressed GATA-5 in the JHOC-5 cell line using an adenovirus system, resulting in that apoptosis was induced and apoptosis-related genes, such as Apaf-1, were up-regulated. These data suggest that GATA-4/-5 may be involved in ovarian carcinogenesis.
Wakana
Kimio
K
Department of Comprehensive Reproductive Medicine, Graduate School of Medicine and Dentistry, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
Akiyama
Yoshimitsu
Y
Aso
Takeshi
T
Yuasa
Yasuhito
Y
D018262:Adenocarcinoma, Clear Cell / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis; D018269:Carcinoma, Endometrioid / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D018284:Cystadenocarcinoma, Serous / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D005260:Female; D050991:GATA4 Transcription Factor / Q000235:genetics*; D050992:GATA5 Transcription Factor / Q000235:genetics*; D050993:GATA6 Transcription Factor / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D020868:Gene Silencing; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic
D004273:DNA, Neoplasm; D050991:GATA4 Transcription Factor; D050992:GATA5 Transcription Factor; D050993:GATA6 Transcription Factor