Involvement of dysadherin and E-cadherin in the development of testicular tumours.
Testicular neoplasms are comprised of a variety of histologically different forms, and their pathogenesis has not been elucidated. Dysadherin is a recently described cell membrane glycoprotein, which has an anticell-cell adhesion function and downregulates E-cadherin. In this study, we examined immunohistochemically the expression of E-cadherin and dysadherin in 120 testicular neoplasms (37 seminomas-26 classic, five spermatocytic and six anaplastic-, 45 embryonal carcinomas, 10 mixed germ cell tumours, two yolk sac tumours, 10 mature and eight immature teratomas and eight non-Hodgkin B-cell lymphomas), clinical stage I. The intensity, the expression pattern and the percentage of neoplastic cell staining was recorded and correlated with the histologic type and vascular/lymphatic invasion. Dysadherin was not expressed in non-neoplastic germ cells, neither in CIS/ITGCNU, but it was highly expressed in all types of germ cell tumours, that demonstrated either embryonic phenotype or somatic differentiation, in most terminally differentiated neoplasms, and in all lymphomas. Dysadherin expression did not correlate with vascular invasion. Increased dysadherin expression was correlated with aberrant E-cadherin expression in most tumours. In 17% of embryonal carcinomas colocalisation of dysadherin and membranous E-cadherin staining was noted. This is the first report on dysadherin expression and its association with E-cadherin in testicular tumours. Since dysadherin is not normally expressed in non-neoplastic testis, it is conceivable that it plays a role in the neoplastic transformation of germ cells. In testicular tumours, as in other neoplasms, dysadherin downregulates E-cadherin expression, at least in part.
Batistatou
A
A
Department of Pathology, University of Ioannina Medical School, Ioannina, Greece. abatista@cc.uoi.gr
Scopa
C D
CD
Ravazoula
P
P
Nakanishi
Y
Y
Peschos
D
D
Agnantis
N J
NJ
Hirohashi
S
S
Charalabopoulos
K A
KA
D000293:Adolescent; D000328:Adult; D000368:Aged; D015820:Cadherins / Q000096:biosynthesis* / Q000502:physiology; D018236:Carcinoma, Embryonal / Q000235:genetics* / Q000503:physiopathology*; D002448:Cell Adhesion; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D007473:Ion Channels; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000503:physiopathology*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000502:physiology; D008840:Microfilament Proteins; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000502:physiology; D013736:Testicular Neoplasms / Q000235:genetics* / Q000503:physiopathology*
D015820:Cadherins; C451410:FXYD5 protein, human; D007473:Ion Channels; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins