Defective repair of radiation-induced DNA damage is complemented by a CHORI-230-65K18 BAC clone on rat chromosome 4.
The Long Evans cinnamon (LEC) rat is highly susceptible to X-irradiation due to defective DNA repair and is thus a model for hepatocellular carcinogenesis. We constructed a bacterial artificial chromosome (BAC) contig of rat chromosome 4 completely covering the region associated with radiation susceptibility. We used transient and stable transfections to demonstrate that defective DNA repair in LEC cells is fully complemented by a 200-kb BAC, CHORI-230-65K18. Further analysis showed that the region associated with radiation susceptibility is located in a 128,543-bp region of 65K18 that includes the known gene Rpn1. However, neither knockdown nor overexpression of Rpn1 indicated that this gene is associated with radiation susceptibility. We also mapped three ESTs (TC523872, TC533727, and CB607546) in the 128,543-bp region, suggesting that 65K18 contains an unknown gene associated with X-ray susceptibility in the LEC rat.
Tsuji
Atsushi B
AB
RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
Sugyo
Aya
A
Sudo
Hitomi
H
Sagara
Masashi
M
Ishikawa
Atsuko
A
Ohtsuki
Marika
M
Kimura
Tomo
T
Ogiu
Toshiaki
T
Miyagishi
Makoto
M
Taira
Kazunari
K
Imai
Takashi
T
Harada
Yoshi-nobu
YN
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002999:Clone Cells; D004249:DNA Damage; D017931:DNA Primers; D004260:DNA Repair; D005816:Genetic Complementation Test; D011836:Radiation Tolerance / Q000235:genetics; D051381:Rats; D014162:Transfection; D014965:X-Rays
D017931:DNA Primers