title: [Effect of bee pollen on development of immune organ of animal].
abstract: OBJECTIVE
  To study on the effect of been pollen on development of immune organ of animal.
  
  
  METHODS
  A total of 144 one day-old broilers were randomly divided into 2 groups, in which each group included 72 chickens. The control group was fed on the basal diet for 42 days, and that of experiment group supplemented 1.5% bee pollen. Six chickens in each group were selected and slaughtered at 7, 14, 21, 28, 35, 42 days respectively, and the thymuses, cloacal bursa and spleens were obtained, weighted, fixed in Bouin liquid and made into paraffin section.
  
  
  RESULTS
  Compared with control group, the weight and the relative weight of thymuses, cloacal bursa and spleens of experiment group increased significantly (P < 0.05) or extremely significantly (P < 0.01). In experiment group, the cortex of thymic lobule, bursa nodule and Periarterial Lymphatic Sheaths thicken obviously; the volume of bursa nodule, splenic nodule and ellipsoid augmented, and the germinal center of splenic nodule were obvious; the thymic corpuscle increased; the plica of cloacal bursa developed well and the degenerating of it retarded.
  
  
  CONCLUSIONS
  The diet supplemented bee pollen could boost the early development of thymus and cloacal bursa, retard the degenerating of cloacal bursa and promote the immune response of spleen.
authors.affiliation: Anhui Science and Technical College, Bengbu 233100, China. wangjue888888@yahoo.com.cn
authors.forename: Jue
authors.initials: J
authors.lastname: Wang
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Jin
authors.forename: Yi-Mei
authors.initials: YM
authors.lastname: Zheng
authors.forename: Sheng-He
authors.initials: SH
authors.lastname: Li
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001516:Bees; D002060:Bursa of Fabricius / Q000033:anatomy & histology / Q000254:growth & development; D002645:Chickens / Q000254:growth & development / Q000276:immunology; D005260:Female; D008297:Male; D009929:Organ Size; D011058:Pollen; D011897:Random Allocation; D013154:Spleen / Q000033:anatomy & histology / Q000254:growth & development; D013950:Thymus Gland / Q000033:anatomy & histology / Q000254:growth & development*