Impact of chromium-contaminated wastewaters on the microbial community of a river.
The influence of chromium on the microbial community structure was analyzed in a river system subjected to long-term chromium contamination, by plating and by sequencing 16S rRNA genes cloned from DNA extracted from the river sediments. We also analyzed the influence of chromium on the ability of the microbial community to resist and reduce Cr(VI) and on its resistance to antibiotics. Shifts in the microbial community structure were analyzed by amplified ribosomal DNA restriction analysis fingerprinting. The isolates obtained were phylogenetically related to Actinobacteria, Firmicutes, Bacteroidetes and Proteobacteria, whereas Acidobacteria and Deltaproteobacteria were only revealed by clone analyses. Cr(VI)-resistant and Cr(VI)-reducing strains were isolated in all sites examined. However, each sample site had a microbial community with a different antibiotic resistance pattern. Our study seems to indicate that in this river ecosystem chromium influenced the microbial communities, altering some of their functional characteristics, such as the percentage of the microbial community able to resist or to reduce Cr(VI) and the phylogenetic groups isolated, but it did not affect the structural diversity. Furthermore, the concentration of Cr(VI) in the sediments could not be correlated with a lower number of bacteria or lower index of generic diversity, neither with the ability of the microbial community to resist or to reduce higher Cr(VI) concentrations.
Branco
Rita
R
Instituto do Ambiente e Vida, Coimbra, Portugal.
Chung
Ana-Paula
AP
Veríssimo
António
A
Morais
Paula V
PV
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification; D002857:Chromium / Q000633:toxicity*; D024881:Drug Resistance, Bacterial; D019015:Geologic Sediments / Q000737:chemistry* / Q000382:microbiology; D010802:Phylogeny; D045483:Rivers / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D000900:Anti-Bacterial Agents; D014874:Water Pollutants, Chemical; D002857:Chromium