title: Efficient production of 2-pyrone 4,6-dicarboxylic acid as a novel polymer-based material from protocatechuate by microbial function.
abstract: Sphingomonas paucimobilis SYK-6, which can degrade various low molecular weight compounds derived from plant polyphenols such as lignin, lignan, and tannin, metabolizes these substances via 2-pyrone-4,6-dicarboxylic acid (PDC). We focused on this metabolic intermediate as a potential raw material for novel, bio-based polymers. We cloned the ligAB and ligC genes of SYK-6, which respectively encode protocatechuate 4,5-dioxygenase and 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase, into a broad host range plasmid vector, pKT230MC. The resulting plasmid, pDVABC, was introduced into the PpY1100 strain of Pseudomonas putida, and we found that PDC could be stably produced from protocatechuate and accumulated. In addition, we examined the efficiency of production of PDC from protocatechuate on a 5-L scale in a Luria-Bertani medium containing 100 mM glucose and determined that PDC was stably produced from protocatechuate to yield 10 g/L or more.
authors.affiliation: Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Masaya
authors.initials: M
authors.lastname: Nakamura
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Shigehara
authors.forename: Kosuke
authors.initials: K
authors.lastname: Sugimura
authors.forename: Eiji
authors.initials: E
authors.lastname: Masai
authors.forename: Seiji
authors.initials: S
authors.lastname: Ohara
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D062385:Hydroxybenzoates / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D011753:Pyrones / Q000378:metabolism*; D020578:Sphingomonas / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D062385:Hydroxybenzoates; D011108:Polymers; D011753:Pyrones; C009091:protocatechuic acid; C024153:alpha-pyrone-4,6-dicarboxylic acid; D049308:Dioxygenases; C022476:protocatechuate 4,5-dioxygenase