DNA polymorphism and molecular subtyping of the capsular gene cluster of group B streptococcus.
Serotyping and other phenotypic methods are often used to characterize the capsular polysaccharide of group B streptococci (GBS). We describe a capsular genotyping method that utilizes PCR of capsular polysaccharide synthesis genes (cps) and restriction enzyme digestion. This method facilitates the detection of DNA polymorphism in cps genes and correlates well with serotyping.
Manning
Shannon D
SD
Microbial Evolution Laboratory, 165 Food Safety & Toxicology Building, Michigan State University, East Lansing, MI 48824, USA.
Lacher
David W
DW
Davies
H Dele
HD
Foxman
Betsy
B
Whittam
Thomas S
TS
D016667:Bacterial Capsules / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005838:Genotype; D006801:Humans; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012703:Serotyping; D013292:Streptococcus agalactiae / Q000145:classification* / Q000235:genetics
D001426:Bacterial Proteins; D004262:DNA Restriction Enzymes