Steatosis-induced proteomic changes in liver mitochondria evidenced by two-dimensional differential in-gel electrophoresis.
Steatosis encompasses the accumulation of droplets of fats into hepatocytes. In this work, we performed a comparative analysis of mitochondrial protein patterns found in wild-type and steatosis-affected liver using the novel technique two-dimensional differential in-gel electrophoresis (2D-DIGE). A total of 56 proteins exhibiting significant difference in their abundances were unambiguously identified. Interestingly, major proteins that regulate generation and consumption of the acetyl-CoA pool were dramatically changed during steatosis. Many proteins involved in the response to oxidative stress were also affected.
Douette
Pierre
P
Laboratory of Bioenergetics, Bât. B6c, Allée de la Chimie 3, 4000 Liège, Belgium.
Navet
Rachel
R
Gerkens
Pascal
P
de Pauw
Edwin
E
Leprince
Pierre
P
Sluse-Goffart
Claudine
C
Sluse
Francis E
FE
D000105:Acetyl Coenzyme A / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005227:Fatty Acids / Q000378:metabolism; D005234:Fatty Liver / Q000473:pathology; D022781:Hepatocytes / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008928:Mitochondria / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism*; D008954:Models, Biological; D018384:Oxidative Stress; D020675:Peroxisomes / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
D005227:Fatty Acids; D000105:Acetyl Coenzyme A; D000255:Adenosine Triphosphate