title: Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region.
abstract: Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.
authors.affiliation: Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, 772 W. 168th Street, Floor 18, New York, NY 10032, USA.
authors.forename: I
authors.initials: I
authors.lastname: Casas
authors.forename: A
authors.initials: A
authors.lastname: Avellon
authors.forename: M
authors.initials: M
authors.lastname: Mosquera
authors.forename: O
authors.initials: O
authors.lastname: Jabado
authors.forename: J E
authors.initials: JE
authors.lastname: Echevarria
authors.forename: R H
authors.initials: RH
authors.lastname: Campos
authors.forename: M
authors.initials: M
authors.lastname: Rewers
authors.forename: P
authors.initials: P
authors.lastname: Perez-Breña
authors.forename: W I
authors.initials: WI
authors.lastname: Lipkin
authors.forename: G
authors.initials: G
authors.lastname: Palacios
mesh_terms: D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D036022:Capsid Proteins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; C018315:hexon capsid protein, Adenovirus