Global synthetic-lethality analysis and yeast functional profiling.
The Saccharomyces genome-deletion project created >5900 'molecularly barcoded' yeast knockout mutants (YKO mutants). The YKO mutant collections have facilitated large-scale analyses of a multitude of mutant phenotypes. For example, both synthetic genetic array (SGA) and synthetic-lethality analysis by microarray (SLAM) methods have been used for synthetic-lethality screens. Global analysis of synthetic lethality promises to identify cellular pathways that 'buffer' each other biologically. The combination of global synthetic-lethality analysis, together with global protein-protein interaction analyses, mRNA expression profiling and functional profiling will, in principle, enable construction of a cellular 'wiring diagram' that will help frame a deeper understanding of human biology and disease.
Ooi
Siew Loon
SL
High Throughput Biology Center, Institute of Genetic Medicine, Department of Biostatistics, Johns Hopkins University School of Medicine, 339 Broadway Research Building, 733 North Broadway, Baltimore, MD 21205, USA.
Pan
Xuewen
X
Peyser
Brian D
BD
Ye
Ping
P
Meluh
Pamela B
PB
Yuan
Daniel S
DS
Irizarry
Rafael A
RA
Bader
Joel S
JS
Spencer
Forrest A
FA
Boeke
Jef D
JD
D002874:Chromosome Mapping; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D005804:Genes, Lethal; D005821:Genetic Techniques; D016681:Genome, Fungal; D020411:Oligonucleotide Array Sequence Analysis; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D012331:RNA, Fungal; D012333:RNA, Messenger