[Study on chemical constituents from Incarvillea arguta and their accelerating PC-12 cell differentiation].
OBJECTIVE
To study chemical constituents of Incarvillea arguta and their accelerating PC-12 cell differentiation.


METHODS
The constituents were isolated and repeatedly purified on silica gel column chromatography, and were identified on the basis of physicochemical and spectroscopic analysis. The neurotrophic activity of different portion and all purified compounds from I. arguta was determined on the model of PC-12 cell.


RESULTS
Five compounds were isolated from BuOH portion of alcohol extraction of I. arguta. Their structures were identified as plantarenaloside (I), 5-hydroxy-4', 6 7-trimethoxy-flavone (II), 4', 5-dihydroxy-6, 7-dimethoxyflavone (III), 4', 5-dihydroxy-7-methoxyflavone (IV), 5-dydroxy-4', 7-dimethoxyflavone (V).


CONCLUSIONS
Compound I is isolated from the plant for the first time and it has neurotrophic activity for PC-12 cell. Compounds II approximately V are isolated from the genus Incarvillea for the first time.
Yu
Zheng-wen
ZW
The Key laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China.
Zhu
Hai-yan
HY
Yang
Xiao-sheng
XS
Sun
Qian-yun
QY
Hao
Xiao-jiang
XJ
D000818:Animals; D047310:Apigenin / Q000302:isolation & purification* / Q000494:pharmacology; D029402:Bignoniaceae / Q000737:chemistry*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D047309:Flavones / Q000302:isolation & purification* / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats
D047309:Flavones; C014049:salvigenin; C044998:apigenin dimethylether; C014568:genkwanin; C007072:cirsimaritin; D047310:Apigenin