Mediastinal node and diaphragmatic targeting after intracavitary injection of avidin/99mTc-blue-biotin-liposome system.
A method for delivering drugs to sites of disease extension in mediastinal nodes is described. Mediastinal node and lymphatic distributions were determined after intracavitary injection of the avidin/biotin-liposome system in normal rats. The effect of the injected dose on lymphatic targeting of liposomes after intraperitoneal injection of (99m)Tc-blue-biotin-liposomes and intrapleural injection of avidin, and vice versa, is presented. Scintigraphic imaging was used to follow the movement of (99m)Tc-blue-biotin-liposomes to determine the pharmacokinetics and organ uptake. Tissue biodistribution studies were performed 22 h after injection of the (99m)Tc-blue-biotin-liposomes. Results indicated that independent of the cavity in which each agent was injected, a dose of 5.0 mg of each agent results in higher mediastinal node targeting (8%-10% ID/Organ) as compared with the injection of a 0.5 mg dose (2%-5% ID/Organ, p < 0.05). Targeting of diaphragm and associated lymphatics was observed when (99m)Tc-blue-biotin-liposomes were injected in peritoneum and avidin in pleural space. In contrast, pleural, and pericardial lymphatic targeting was observed when (99m)Tc-blue-biotin-liposomes were injected in pleural space and avidin in peritoneum. Intracavitary injection of the avidin/biotin-liposome system could potentially be used for the delivery of prophylactic drugs that could reduce tumor metastasis and infection spread to mediastinal nodes.
Medina
Luis A
LA
Instituto de Fisica, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico. medini@fisica.unam.mx
Calixto
Sergio M
SM
Klipper
Robert
R
Li
Yang
Y
Phillips
William T
WT
Goins
Beth
B
D000818:Animals; D001360:Avidin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001710:Biotin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D003964:Diaphragm / Q000378:metabolism; D007267:Injections; D007274:Injections, Intraperitoneal; D008081:Liposomes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008198:Lymph Nodes / Q000378:metabolism; D042601:Lymphatic Vessels / Q000378:metabolism; D008297:Male; D008482:Mediastinum; D035422:Pleural Cavity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013667:Technetium
D008081:Liposomes; D001360:Avidin; D001710:Biotin; D013667:Technetium