Immobilization of glucose oxidase in conducting graft copolymers and determination of glucose amount in orange juices with enzyme electrodes.
Glucose oxidase was immobilized in conducting copolymers of three different types of poly(methyl methacrylate-co-thienyl methacrylate). Immobilization of enzyme was carried out by the entrapment in conducting polymers during electrochemical polymerization of pyrrole on the copolymer electrodes. Maximum reaction rate, Michaelis-Menten constants, temperature, pH and operational stabilities were determined for immobilized enzyme. The amount of glucose in orange juices of Turkey was investigated by using enzyme electrodes.
Yildiz
Hüseyin Bekir
HB
Department of Chemistry, Middle East Technical University, 06531 Ankara, Turkey.
Kiralp
Senem
S
Toppare
Levent
L
Yağci
Yusuf
Y
D001628:Beverages / Q000032:analysis; D015374:Biosensing Techniques; D032084:Citrus sinensis / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry
D004800:Enzymes, Immobilized; D011108:Polymers; D019904:Polymethyl Methacrylate; D005949:Glucose Oxidase; D005947:Glucose