[Genomic analysis of a familial clustering of chronic hepatitis B patients].
OBJECTIVE
To investigate the peripheral blood monocyte (PBMC) gene expression profile in a familial clustering of patients with chronic hepatitis B (CHB).


METHODS
cRNA prepared from PBMC in a family with 5 CHB patients and 4 normal controls was hybridized to high-density oligouncleotide arrays (HG-U133A 2.0 Human GeneChips, Affymetrix), which interrogate the expression of approximately 22,000 human ESTs. Primary image obtained from scanning was analysed with a DNT software package. Real-time PCR was employed to confirm the gene chip results.


RESULTS
55 genes out of 22,000 ESTs were identified differently. Among the 55 genes 14 showed increased expression and 41 showed decreased expression in the familial clustering CHB patients compared with those in normal controls. Most of the genes (57%) were involved in immunity, inflammation, apoptosis, signaling transduction, and cell cycle.


CONCLUSIONS
These results suggest that the hosts with this broad range of gene expression alterations are susceptible to hepatic B infection.
Zhao
Ying-ren
YR
Department of Infectious Diseases, First Hospital of Xi'an JiaoTong University, Xi'an 710061, China. zhaoyingren@163.com
He
Ying-li
YL
Zhang
Shu-lin
SL
Yang
Yuan
Y
Liu
Min
M
Jin
Yan
Y
Liu
Jin-feng
JF
D016000:Cluster Analysis; D005192:Family Health; D005260:Female; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D016678:Genome; D019694:Hepatitis B, Chronic / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010375:Pedigree