Characterization of the focal adhesion complex in human non-small cell lung cancer cell lines.
BACKGROUND
The important metastatic potential of lung cancers is directly correlated with cell adhesion. Cell-extracellular matrix interactions occur in specialized structures termed focal adhesion (FA) complexes. Our aims were to investigate: (i) the expression of the major FA components in three lung cancer cell lines (non metastatic: A549, or metastatic: Calu-1 and H460), (ii) the modifications of the FA complex occurring when apoptosis was induced by Vinorelbine in the A549 cells.


METHODS
The FA complex was characterized by flow cytometry, immunocytochemical staining and Western blot.


RESULTS
The expressions of alpha3, betsaP, paxillin, p-paxillin and Grb2 varied depending on the histological type of the tumor. In apoptotic cells, the expressions of the PYK2, p-p38, PI3K and Grb2 adhesion proteins were increased.


CONCLUSIONS
Our data suggest that these adhesion proteins may be implicated in the transduction of death signals.
Forest
Valérie
V
UPRES-EA3063, Faculté de Médecine Jacques Lisfranc, Saint-Etienne, France. Valerie.forest@univ-st-etienne.fr
Campos
Lydia
L
Vergnon
Jean-Michel
JM
Cornillon
Jérome
J
Guyotat
Denis
D
D017209:Apoptosis / Q000502:physiology; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D051418:Focal Adhesion Kinase 2 / Q000096:biosynthesis; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000096:biosynthesis; D022001:Focal Adhesions / Q000502:physiology*; D051380:GRB2 Adaptor Protein / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D039422:Integrin alpha3 / Q000096:biosynthesis; D019012:Integrin beta1 / Q000096:biosynthesis; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D051419:Paxillin / Q000096:biosynthesis; D019869:Phosphatidylinositol 3-Kinases / Q000096:biosynthesis
D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D039422:Integrin alpha3; D019012:Integrin beta1; D051419:Paxillin; D019869:Phosphatidylinositol 3-Kinases; D051418:Focal Adhesion Kinase 2; D051416:Focal Adhesion Protein-Tyrosine Kinases