Regional specificity of chandelier neuron axon terminal alterations in schizophrenia.
BACKGROUND
The axon terminals of GABAergic chandelier cells form linear arrays, termed cartridges, that synapse on the axon initial segment of neocortical pyramidal cells. These cartridges are immunoreactive for the GABA membrane transporter-1, and the density of GABA membrane transporter-1-immunoreactive cartridges in the prefrontal cortex has been reported to be reduced in schizophrenia. The goal of this study was to determine if reductions in the density of GABA membrane transporter-1-immunoreactive cartridges in schizophrenia are restricted to the prefrontal cortex.


METHODS
Relative GABA membrane transporter-1-immunoreactive cartridge density was determined in auditory association area 42, a region previously implicated in the pathophysiology of schizophrenia, in 14 matched pairs of subjects with schizophrenia and normal comparison subjects. The results were compared with similar data from prefrontal area 46 in the same subjects.


RESULTS
Mean GABA membrane transporter-1-immunoreactive cartridge density in area 42 was decreased by 9.8% in layers II-IIIa, and by 11.9% in layer VI in subjects with schizophrenia, although these differences did not achieve statistical significance. However, the magnitude of the reductions in the density of GABA membrane transporter-1-immunoreactive cartridges in area 42 of the subjects with schizophrenia was not significantly smaller than those in area 46.


CONCLUSIONS
In subjects with schizophrenia, alterations in chandelier neuron axon cartridges appear to be more marked in the prefrontal cortex than in another cortical region implicated in the illness, although such changes might not be restricted to the prefrontal cortex.
Konopaske
G T
GT
Department of Psychiatry, University of Pittsburgh, PA 15213, USA.
Sweet
R A
RA
Wu
Q
Q
Sampson
A
A
Lewis
D A
DA
D000328:Adult; D000368:Aged; D001303:Auditory Cortex / Q000166:cytology / Q000378:metabolism; D003627:Data Interpretation, Statistical; D005260:Female; D050485:GABA Plasma Membrane Transport Proteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D017397:Prefrontal Cortex / Q000166:cytology / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism / Q000502:physiology*; D017966:Pyramidal Cells / Q000378:metabolism / Q000502:physiology; D012559:Schizophrenia / Q000378:metabolism / Q000503:physiopathology*
D050485:GABA Plasma Membrane Transport Proteins; C493413:SLC6A1 protein, human