title: Repression of polymerase I-mediated gene expression at Trypanosoma brucei telomeres.
abstract: The African trypanosome, Trypanosoma brucei, is a flagellated pathogenic protozoan that branched early from the eukaryotic lineage. Unusually, it uses RNA polymerase I (Pol I) for mono-telomeric expression of variant surface glycoprotein (VSG) genes in bloodstream-form cells. Many other subtelomeric VSG genes are reversibly repressed, but no repressive DNA sequence has been identified in any trypanosomatid. Here, we show that artificially seeded de novo telomeres repress Pol I-dependent gene expression in mammalian bloodstream and insect life-cycle stages of T. brucei. In a telomeric VSG expression site, repression spreads further along the chromosome and this effect is specific to the bloodstream stage. We also show that de novo telomere extension is telomerase dependent and that the rate of extension correlates with the expression level of the adjacent gene. Our results show constitutive telomeric repression in T. brucei and indicate that an enhanced, developmental stage-specific repression mechanism controls antigenic variation.
authors.affiliation: London School of Hygiene & Tropical Medicine, London, UK.
authors.forename: Lucy
authors.initials: L
authors.lastname: Glover
authors.forename: David
authors.initials: D
authors.lastname: Horn
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012318:RNA Polymerase I / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism; D016615:Telomere / Q000235:genetics / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000378:metabolism; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics / Q000378:metabolism
chemical_list: D015800:Protozoan Proteins; D014643:Variant Surface Glycoproteins, Trypanosoma; D019098:Telomerase; D012318:RNA Polymerase I