Cardioprotective activity of alcoholic extract of Tinospora cordifolia in ischemia-reperfusion induced myocardial infarction in rats.
It has been suggested that the beneficial effects of reperfusing the myocardium might be in part reversed by the occurrence of reperfusion injury. Oxidative stress was suggested to be implicating in the pathogenesis of ischemia-reperfusion (I/R) injury. Many antioxidative plants were shown to be cardioprotective in experimental models of myocardial ischemia-reperfusion (I/R) injury. The present study was designed to investigate the effects of pretreatment with alcoholic extract of Tinospora cordifolia in an in vivo rat model. The model adopted was that of surgically-induced myocardial ischemia, performed by means of left anterior descending coronary artery occlusion (LAD) for 30 min followed by reperfusion for another 4 h. Infarct size was measured by using the staining agent TTC (2,3,5-triphenyl tetrazolium chloride). Lipid peroxide levels in serum and in heart tissue were estimated spectrophotometrically by the methods developed by Yagi and Ohkawa et al. respectively. A lead II electrocardiogram was monitored at various intervals throughout the experiment. A dose dependent reduction in infarct size and in lipid peroxide levels of serum and heart tissue were observed with the prior treatment of T. cordifolia with various doses for 7 d compared to control animals. Hence, the present study suggests the cardioprotective activity of T. cordifolia in limiting ischemia-reperfusion induced myocardial infarction.
Rao
Pragada Rajeswara
PR
Pharmacology Division, Department of Pharmaceutical Sciences, Andhra University, Pradesh, India. rajeswararaopragada@rediffmail.com
Kumar
Veeravalli Krishna
VK
Viswanath
Routhu Kasi
RK
Subbaraju
Gottumukkala Venkata
GV
D000438:Alcohols / Q000737:chemistry; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D007441:Intubation, Gastrointestinal; D008297:Male; D008315:Malondialdehyde / Q000037:antagonists & inhibitors / Q000097:blood / Q000737:chemistry; D009203:Myocardial Infarction / Q000209:etiology / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000150:complications / Q000188:drug therapy*; D009206:Myocardium / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D017257:Ramipril / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D031610:Tinospora / Q000737:chemistry*
D000438:Alcohols; D000959:Antihypertensive Agents; D002316:Cardiotonic Agents; D010936:Plant Extracts; D008315:Malondialdehyde; D017257:Ramipril