[Study of estradiol on treatment of preeclampsia in rat model].
OBJECTIVE
To evaluate whether estradiol can inhibit and cure the inflammation of experimental preeclampsia in rats.


METHODS
Experimental preeclampsia was induced in 14-day-pregnant rats by infusion of endotoxin (1.0 microg/kg). Rats with normal pregnancy were infused with sodium chloride solution. A group of preeclampsia rats was injected with 17beta-estradiol (17beta-E(2), 1 mg.kg(-1).d(-1)). Blood pressure, albuminuria, inflammation associated adhesion molecule CD(49d) and tumor necrosis factor-alpha (TNF-alpha) were assessed.


RESULTS
On pregnant day 19, for normal pregnancy group (group C) the blood pressure was (120.4 +/- 2.0) mm Hg (1 mm Hg = 0.133 kPa), urinary protein (0.47 +/- 0.06) mg/24 hours; for experimental preeclampsia group (group A) blood pressure was (134.2 +/- 2.4) mm Hg, urinary protein (0.79 +/- 0.10) mg/24 hours; for experimental preeclampsia with 17beta-E(2) treatment group (group B) blood pressure was (123.3 +/- 1.7) mm Hg, urinary protein (0.51 +/- 0.08) mg/24 hours. A significant increase of blood pressure and urinary albumin was observed in group A. CD(49d) expression and TNF-alpha concentration were also increased. 17beta-E(2) reduced the expression of CD(49d), concentration of TNF-alpha, blood pressure and albuminuria of experimental preeclampsia. However, the weight of fetuses in 17beta-E(2) treatment group were less than that in other groups.


CONCLUSIONS
17beta-E(2) can improve the symptoms of experimental preeclampsia, but its effects on fetus need to be further studied.
Wang
Xue-yan
XY
Department of Obstetrics, Sichuan Provincial Hospital for Women and Children, Chengdu 610031, China.
Xiong
Qing
Q
Wang
Chao
C
Xiao
Bing
B
Zhou
Shu
S
Zhou
Rong
R
Xing
Ai-yun
AY
D000818:Animals; D001794:Blood Pressure; D004958:Estradiol / Q000627:therapeutic use*; D005260:Female; D039441:Integrin alpha4 / Q000096:biosynthesis; D008297:Male; D011225:Pre-Eclampsia / Q000188:drug therapy* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D014409:Tumor Necrosis Factor-alpha; D039441:Integrin alpha4; D004958:Estradiol