[Experimental study on the reconstruction of bone defects in vivo and the osteoinduction with degradation products of alpha-tricalcium phosphate cements in vitro].
OBJECTIVE
This study was aimed to clarify the characteristics of alpha-tricalcium phosphate cements in reconstructing bone defects and the action of degradation products of cements in vitro.


METHODS
The defect (diameter 1.5 cm) was created in the calvaria of rabbit. Alpha-tricalcium phosphate cements were planted in the treatment groups. All groups were observed by histological examination and roentgenography. Bone marrow stromal cells (BMSc) were cultured in the degradation products of cements in vitro, and were observed by means of cytology and ALP.


RESULTS
Degradation of artificial bone, new bone formation, and osteogenesis between artificial bone particles were observed at 12 weeks after implantation. Osteoinduction was observed in vitro.


CONCLUSIONS
Alpha-tricalcium phosphate cements have good biocompatibility, osteoinductivity and degradability, and are ideal bone substitute material for reconstruction.
Song
Hong-Jie
HJ
Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Liang
Xing
X
Luo
Xiao-Qiu
XQ
Chen
Ming
M
Sun
Hui-Qiang
HQ
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology* / Q000627:therapeutic use; D001673:Biodegradation, Environmental; D001854:Bone Marrow Cells / Q000473:pathology; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000494:pharmacology* / Q000627:therapeutic use; D002478:Cells, Cultured; D005260:Female; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D011817:Rabbits; D011897:Random Allocation; D019651:Plastic Surgery Procedures; D012886:Skull / Q000293:injuries* / Q000473:pathology / Q000601:surgery
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate