Localization and changes of diamine oxidase during cardiopulmonary bypass in rabbits.
BACKGROUND
We previously observed increased serum diamine oxidase activity during clinical cardiopulmonary bypass, indicating small intestinal mucosal ischemia followed by bacterial translocation.


METHODS
In seven female rabbits undergoing cardiopulmonary bypass for 1 h, we analyzed the localization of diamine oxidase immunohistochemically, and measured its activity in serum and abdominal organs before and after cardiopulmonary bypass (CPB).


RESULTS
Preoperatively, diamine oxidase activity and immunoreactivity were high in the small intestine, localized to villus tips. Serum activity increased significantly after CPB, whereas small intestinal diamine oxidase decreased with mucosal injury.


CONCLUSIONS
In this model serum diamine oxidase activity appeared to reflect CPB induced intestinal mucosal injury.
Tsunooka
Nobuo
N
Second Department of Surgery, Ehime University School of Medicine, Ehime, Japan. tsunooka@m.ehime-u.ac.jp
Maeyama
Kazutaka
K
Nakagawa
Hiromichi
H
Doi
Takashi
T
Horiuchi
Atsushi
A
Miyauchi
Katsutoshi
K
Watanabe
Yuji
Y
Imagawa
Hiroshi
H
Kawachi
Kanji
K
D006631:Amine Oxidase (Copper-Containing) / Q000032:analysis* / Q000378:metabolism*; D000818:Animals; D002315:Cardiopulmonary Bypass / Q000009:adverse effects* / Q000662:veterinary; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000201:enzymology* / Q000473:pathology; D011817:Rabbits; D014018:Tissue Distribution
D006631:Amine Oxidase (Copper-Containing)