title: Effect of the infectious laryngotracheitis virus (ILTV) glycoprotein G on virus attachment, penetration, growth curve and direct cell-to-cell spread.
abstract: The secreted alphaherpesvirus glycoprotein G (gG) works differently from other proteins. Analysis of the role of ILTV gG in virus attachment, penetration, direct cell-to-cell spread (CTCS) and the growth curve showed that gG or its antibody had no effect on ILTV attachment and penetration and that the gG antibody reduced the virus plaque size and the one-step growth curve on chicken embryo liver (CEL) cells, but gG did not affect the virus plaque size or the one-step growth curve on CEL cells. Laser scanning confocal microscopy (LSCM) detection showed that ILTV gG is located in the perinuclear region and the membrane of the CEL cells. These results suggested that ILTV gG might contribute to direct cell-to-cell transmission.
authors.affiliation: Lab for Animal Molecular Virology, College of Biological Sciences, China Agricultural University, Beijing, 100094, China.
authors.forename: Zhaogang
authors.initials: Z
authors.lastname: Sun
authors.forename: Manfu
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000821:virology; D002642:Chick Embryo; D003593:Cytoplasm / Q000378:metabolism / Q000821:virology; D006023:Glycoproteins / Q000378:metabolism / Q000502:physiology*; D006566:Herpesviridae Infections / Q000635:transmission* / Q000821:virology; D001352:Herpesvirus 1, Gallid / Q000254:growth & development* / Q000472:pathogenicity*; D008099:Liver / Q000196:embryology / Q000821:virology; D014759:Viral Envelope Proteins / Q000378:metabolism / Q000502:physiology*; D010948:Viral Plaque Assay; D014771:Virion / Q000254:growth & development / Q000472:pathogenicity; D053585:Virus Attachment; D053586:Virus Internalization
chemical_list: D006023:Glycoproteins; D014759:Viral Envelope Proteins