Rapid and reliable method of extracting DNA and RNA from sweetpotato, Ipomoea batatas (L). Lam.
A quick, simple and reliable method of extracting DNA from sweetpotato (Ipomoea batatas (L.) Lam.) has been developed. The method was applied successfully for extraction of total DNA from leaves and total RNA from leaves and various tissues. The yield of DNA extracted by this procedure was high (about 1 mg/g leaf tissue). The extracted DNA was completely digested by restriction endonucleases indicating the absence of common contaminating compounds. The absorbancy ratios of A260/A230 and A260/A280 of isolated RNA were approx. 2 and the yield was about 0.2 mg/g fresh wt. CIPK and tublin genes were successfully amplified by RT-PCR, suggesting the integrity of isolated RNA. The total DNA and RNA isolated by this method was of sufficient quality for subsequent molecular analysis.
Kim
Sun-Hyung
SH
Research Institute of Bioresource and Biotechnology, Ishikawa Prefectural University, Suematu, 921-8836 Nonoichi, Ishikawa, Japan. mongdal@ishikawa-pu.ac.jp
Hamada
Tatsuro
T
D019840:2-Propanol / Q000737:chemistry; D015139:Blotting, Southern; D002725:Chloroform / Q000737:chemistry; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D027723:Ipomoea batatas / Q000737:chemistry / Q000235:genetics*; D018021:Lithium Chloride / Q000737:chemistry; D000439:Pentanols / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet; D014404:Tubulin / Q000235:genetics
D018744:DNA, Plant; D000439:Pentanols; D018749:RNA, Plant; D012335:RNA, Ribosomal; D014404:Tubulin; D002725:Chloroform; C029683:isopentyl alcohol; D011494:Protein Kinases; D018021:Lithium Chloride; D019840:2-Propanol