[Inactivation of aflatoxin B1 and aflatoxicol through traditional "nixtamalización" of corn and their regeneration by acidification of corn dough].
OBJECTIVE
To ratify the effect of traditional "nixtamalización" upon aflatoxin in corn, to identify the residual compound in dough, to evaluate its toxicity, and to verify the effect of acidic treatment on the regeneration of aflatoxin.


METHODS
Corn with and without aflatoxin was treated separately with lime and heat to obtain two types of dough. Aflatoxin and the residual compound were quantified by high pressure liquid chromatography (HPLC) and toxicity was evaluated in eight day old chicks.


RESULTS
"Nixtamalización" destroyed AFBI (96%) and aflatoxicol (70%). The residual compound was identified as AFBI. Experimental animals died by ingestion of 260 microg of AFBI Dough containing residual aflatoxin was not toxic. Acidic treatment did not increase aflatoxin concentration,nor aflatoxicol.


CONCLUSIONS
The results support the use of traditional "nixtamalización" as a means to inactivate aflatoxin in corn and that acidic treatment prevents its regeneration in dough.
Anguiano-Ruvalcaba
Gloria Laura
GL
Laboratorio de Micotoxinas, Departamento de Biotecnología y Bioquímica, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Guanajuato, México.
Verver y Vargas-Cortina
Aurora
A
Guzmán-de Peña
Doralinda
D
D016604:Aflatoxin B1 / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000348:Aflatoxins / Q000032:analysis / Q000037:antagonists & inhibitors*; D005511:Food Handling; D006863:Hydrogen-Ion Concentration; D011042:Poisons / Q000032:analysis / Q000633:toxicity*; D003313:Zea mays / Q000737:chemistry
D000348:Aflatoxins; D011042:Poisons; C012170:aflatoxicol; D016604:Aflatoxin B1