[Experimental study on action of acupoint embedding thread on ANCA in the rat of ulcerative colitis].
OBJECTIVE
To observe therapeutic effect of acupoint embedding thread therapy on ulcerative colitis and to study the mechanism.


METHODS
The rat model of ulcerative colitis was developed with 2,4-dinitrochlorobenzene method. The 20th day after the end of model development, they were randomly divided into a model group, a salicylazosulfapyridine (SASP) treatment group and an acupoint embedding thread therapy group. The SASP treatment group were treated with intragastrical perfusion of 4.5% SASP suspension, and the acupoint embedding thread therapy group were treated with embedding thread at "Zusanli" (ST 36) and "Shangjuxu" (ST 37). Anti-neutrophil cytoplasmic antibody (ANCA) was detected with indirect immunofluorescence, and the pathological changes were investigated with pathological tissue section technique.


RESULTS
The positive rate of ANCA reached to 62.5% in the rat with ulcerative colitis with significant differences among the 3 groups. After treatment, the positive rate of ANCA was decreased in the two treatment groups (P < 0.05, P < 0.01).


CONCLUSIONS
Acupoint embedding thread therapy has definite therapeutic effect on ulcerative colitis and the mechanism is possibly related with the decrease of ANCA.
Zou
Yang
Y
Provincial Center for Clinical Laboratory, Guizhou Provincial People's Hospital, Guiyang 550002, China. DXL@public.GZ.cn
Zou
Zhu-Liang
ZL
Yang
Xiao-Fang
XF
Zhou
Xiao-Quan
XQ
Ou
Gui-Zhen
GZ
D015669:Acupuncture Points; D000818:Animals; D019268:Antibodies, Antineutrophil Cytoplasmic; D003093:Colitis, Ulcerative / Q000628:therapy; D051381:Rats
D019268:Antibodies, Antineutrophil Cytoplasmic