Identification of all alpha1-adrenoceptor subtypes in rat lung.
The function of lung tissue is regulated via a release of neurotransmitters from autonomic nerves. The neurotransmitters of sympathetic nervous system, adrenaline and noradrenaline, activate both alpha-adrenoceptors and beta-adrenoceptors. Although the function and expression of beta-adrenoceptors can be considered major, some doubts exist about the function and expression of alpha1-adrenoceptor subtypes in the lung tissue. Therefore a set of competition binding experiments was employed in order to discriminate between the alpha1-adrenoceptor binding site subtypes in the rat lung. We identified three subpopulations of alpha1-adrenoceptor binding sites in the rat lung (alpha1A, alpha1B and alpha1D).
Nováková
M
M
Institute of Physiology, 1st Faculty of Medicine, Charles University, Albertov 5, 128 00 Prague, Czech Republic. noma@1f1.cuni.cz
Myslivecek
J
J
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D005786:Gene Expression Regulation; D008168:Lung / Q000378:metabolism*; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D018340:Receptors, Adrenergic, alpha-1 / Q000096:biosynthesis*
C547715:Adra1a protein, rat; C547718:Adra1b protein, rat; C547721:Adra1d protein, rat; D018377:Neurotransmitter Agents; D018340:Receptors, Adrenergic, alpha-1