Characterization of a novel satellite virus and a strain of Himetobi P virus (Dicistroviridae) from the brown planthopper, Nilaparvata lugens.
Two distinct spherical virus-like particles were purified from the brown planthopper, Nilaparvata lugens. One was a geographical isolate of Himetobi P virus (Cripavirus, Dicistroviridae). The other was 30 nm in diameter and contained positive-stranded RNA. The RNA was 1647 nucleotides in length and encoded only its own capsid protein, indicating that this particle is a satellite virus. Transmission tests showed that the satellite was transmitted vertically; however, its helper virus was unknown. We named this satellite Nilaparvata lugens commensal X virus (NLCXV).
Nakashima
Nobuhiko
N
National Institute of Agrobiological Sciences, Ohwashi, Tsukuba, Ibaraki 305-8634, Japan.
Kawahara
Nobuyuki
N
Omura
Toshihiro
T
Noda
Hiroaki
H
D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006430:Hemiptera / Q000821:virology*; D018445:Infectious Disease Transmission, Vertical; D007304:Insect Viruses / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D012087:Reoviridae / Q000235:genetics; D012088:Reoviridae Infections / Q000635:transmission*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012526:Satellite Viruses / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure
D036022:Capsid Proteins; D012367:RNA, Viral