Leptin-mediated inhibition of the insulin-stimulated increase in fatty acid uptake in differentiated 3T3-L1 adipocytes.
The effects of insulin and leptin on fatty acid uptake in differentiated (adipocytes) and undifferentiated 3T3-L1 cells were investigated. It was demonstrated that in undifferentiated 3T3-L1 cells, insulin and leptin have no effect on fatty acid uptake. In differentiated 3T3-L1 adipocytes, insulin had a concentration-dependent stimulatory effect on fatty acid uptake, whereas leptin on its own had no effect. Leptin, when coincubated with 10 nmol/L insulin, resulted in a concentration-dependent inhibition of the insulin-stimulated fatty acid uptake in differentiated 3T3-L1 cells. These results indicate that leptin has a direct inhibitory effect on the stimulation of fatty acid uptake by insulin in differentiated murine adipocytes.
Ho
Michael
M
Department of Biology, Cambridge Biotechnology Ltd, Babraham Research Campus, Cambridge CB2 1XJ , UK.
Foxall
Susan
S
Higginbottom
Michael
M
Donofrio
David M
DM
Liao
Jinfang
J
Richardson
Peter J
PJ
Maneuf
Yannick P
YP
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D020738:Leptin / Q000494:pharmacology*; D051379:Mice; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence
D005227:Fatty Acids; D007004:Hypoglycemic Agents; D007328:Insulin; D020738:Leptin; D012313:RNA; D004247:DNA