Different skeletal regional response to continuous brain infusion of leptin in the rat.
This study was designed to evaluate whether or not continuous intracerebroventricular infusion of leptin (1.5 microg/rat/24 h, for 28 days) produced different regional response on the skeleton of growing rats. Leptin reduce the accretion of total femoral bone mineral content (BMC) and density (BMD). This effect was related to a reduction of metaphyseal femur as no changes were detected in the diaphysis. Despite the reduced accretion in the volumetric of both femur and tibia compared to controls, leptin had no significant effects on the lumbar vertebrae. Urine deoxypyrydinoline and serum osteocalcin remained more elevated in the leptin-treated group as compared to controls. The results demonstrate that long-term central infusion of leptin activates bone remodeling with a negative balance. Leptin induces distinct responses in the different structure of bone and in the axial and appendicular skeleton.
Guidobono
F
F
Department of Pharmacology, Chemotherapy and Medical Toxicology, University of Milan, Italy. francesca.guidobono@unimi.it
Pagani
F
F
Sibilia
V
V
Netti
C
C
Lattuada
N
N
Rapetti
D
D
Mrak
E
E
Villa
I
I
Cavani
F
F
Bertoni
L
L
Palumbo
C
C
Ferretti
M
M
Marotti
G
G
Rubinacci
A
A
D000818:Animals; D001835:Body Weight; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D020738:Leptin / Q000008:administration & dosage* / Q000378:metabolism; D008297:Male; D015675:Osteocalcin / Q000097:blood; D010455:Peptides / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014057:Tomography, X-Ray Computed
D020738:Leptin; D010455:Peptides; D015675:Osteocalcin