Survey of human immunodeficiency virus (HIV)-seropositive patients with mycobacterial infection in Japan.
OBJECTIVE
To assess DNA polymorphisms in mycobacterial isolates obtained from human immunodeficiency virus (HIV)-seropositive patients with tuberculosis in Japan from 1996 to 2003.


METHODS
Restriction fragment length polymorphisms (RFLP) from Mycobacterium tuberculosis and Mycobacterium avium isolates obtained from individual seropositive patients with tuberculosis (n=78) were analysed with the use of IS6110 and (CGG)(5) or IS1245 and IS1311, respectively, as markers. As a control, the same procedures were applied to isolates from HIV-seronegative tuberculosis patients (n=87).


RESULTS
Of 86 mycobacterial strains, M. tuberculosis, M. avium and Mycobacterium chelonae were identified in 48 (55.8%), 36 (41.9%) and 2 (2.3%) isolates, respectively. The obtained RFLP patterns of M. tuberculosis isolates from both the HIV-seropositive and -seronegative groups were variable, suggesting no obvious clustering among the isolates. Similar results were obtained in isolates of M. avium.


CONCLUSIONS
This is the first report on the molecular epidemiology of Mycobacterium spp. isolated from HIV-seropositive patients in Japan. The results indicate that no particular clones of M. tuberculosis or M. avium prevail in HIV-seropositive patients in Japan. Further monitoring of mycobacterial infection associated with HIV infection in Japan should be continued.
Otsuka
Yayoi
Y
International Medical Center of Japan, Toyama 1-21-1, Shinjuku-ku, Tokyo 162-8655, Japan.
Fujino
Tomoko
T
Mori
Namiko
N
Sekiguchi
Jun-ichiro
J
Toyota
Emiko
E
Saruta
Katsutoshi
K
Kikuchi
Yoshihiro
Y
Sasaki
Yuka
Y
Ajisawa
Atsushi
A
Otsuka
Yoshito
Y
Nagai
Hideaki
H
Takahara
Makoto
M
Saka
Hideo
H
Shirasaka
Takuma
T
Yamashita
Yasuki
Y
Kiyosuke
Makiko
M
Koga
Hideyuki
H
Oka
Shinichi
S
Kimura
Satoshi
S
Mori
Toru
T
Kuratsuji
Tadatoshi
T
Kirikae
Teruo
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D016172:DNA Fingerprinting; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000453:epidemiology*; D018023:HIV Seronegativity; D006679:HIV Seropositivity; D006306:Health Surveys; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009164:Mycobacterium Infections / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology; D009162:Mycobacterium avium / Q000235:genetics* / Q000302:isolation & purification; D016926:Mycobacterium chelonae / Q000235:genetics / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence