Development of a microcapillary column for detecting targeted messenger RNA molecules.
A capillary column in a rapid-flow system has been developed for detecting targeted messenger RNA (mRNA) molecules. The column has a structure made of two beds-one bed of porous microbeads and one bed of microbeads with a polythymidine base sequence. The targeted eukaryotic mRNA molecules are detected by two-step hybridization (sandwich hybridization) composed of polyadenosine selection of mRNA molecules and formation of a probe-target (targeted mRNA) hybrid. The sandwich hybridization, which is accomplished within 1 h, was tested using synthetic polydeoxynucleotides. Ten picomoles of the targeted polydeoxynucleotide were detected.
Ohnishi
Michihiro
M
Life Science Laboratory, Materials Laboratories, Sony Corporation, 6-7-35 Kitashinagawa Shinagawa-ku, Tokyo 141-0001, Japan. Michihiro.Ohnishi@jp.sony.com
D001483:Base Sequence; D002853:Chromatography, Liquid / Q000295:instrumentation*; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D015347:RNA Probes; D012333:RNA, Messenger