title: Estimating mutagenic compounds generated during photolysis of fenitrothion--by HPLC fractionation followed by mutagenicity testing and high-resolution GC-MS analysis.
abstract: We aimed to: (1) evaluate the change in mutagenicity of a fenitrothion-containing solution during photolysis and (2) elucidate mutagenic compounds that were possible major contributors to mutagenicity. A batch test involving irradiation by natural sunlight was conducted on the solution, and then HPLC fractionation, mutagenicity testing, and gas chromatography-mass spectrometry (GC-MS) analysis were performed on the irradiated solution. During the 15-day photolysis, fenitrothion was almost completely decomposed, and 34 transformed products (TPs) were generated. Photolysis decreased the mutagenicity of the fenitrothion-containing solution for base-pair-substitution-detecting tester strains (YG1026 and YG1029) but increased mutagenicity for frameshift-detecting tester strains (YG1021 and YG1024). One TP was identified as a potential source of the increased mutagenicity; its molecular formula was estimated to be (CH(3)O)(2)PS-O-C(8)H(6)NO.
authors.affiliation: Department of Civil Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. taku_m@cc.gifu.ac.jp
authors.forename: Taku
authors.initials: T
authors.lastname: Matsushita
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Matsui
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Matsui
mesh_terms: D000818:Animals; D020137:Base Pair Mismatch; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D005278:Fenitrothion / Q000378:metabolism / Q000528:radiation effects* / Q000633:toxicity; D016368:Frameshift Mutation; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D007306:Insecticides / Q000378:metabolism / Q000528:radiation effects* / Q000633:toxicity; D008099:Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity*; D010782:Photolysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism; D013472:Sunlight
chemical_list: D007306:Insecticides; D009153:Mutagens; D005278:Fenitrothion