Structure and nucleotide polymorphisms in pig uncoupling protein 2 and 3 genes.
Uncoupling proteins (UCPs) are mitochondrial membrane transporters, acting as an uncoupler in oxidative phosphorylation. In this study, we designed 11 primer sets based on the human and mouse UCP2, UCP3 sequences and successfully amplified full regions of porcine UCP2 and UCP3 by polymerase chain reactions (PCR). Comparison of the UCP2 and UCP3 genic structures revealed a highly conservative region was putatively presented, showing the second transmembrane domain may be the UCPs' cardinal function region. Altogether 23 nucleotide polymorphisms of UCP2 and UCP3 genes were discovered in Yorkshire, Wuzhishan, and Lepinghua pigs. These polymorphisms included 3 missense mutations, 16 intronic substitutions, and 4 intronic deletions. The substitution of Ala-55-Val in UCP2 is actually the most common mutation in human. We also calculated genotypic frequencies of five polymorphisms in three pig breeds.
Li
Hanjie
H
College of Animal Science and Technology, State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, P.R. China.
Li
Yanhua
Y
Zhao
Xingbo
X
Li
Ning
N
Wu
Changxin
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D007473:Ion Channels; D026901:Membrane Transport Proteins / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3
D002352:Carrier Proteins; D007473:Ion Channels; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; C000604389:UCP2 protein, human; C000604392:UCP3 protein, human; C000604390:Ucp2 protein, mouse; C000604393:Ucp3 protein, mouse; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3; D004247:DNA