title: Nucleotide sequence and variation of IGF2 gene exon 6 in Bos taurus and Bos indicus cattle.
abstract: The major assumption of this study is that polymorphism of a gene could be used to investigate its allele-specific expression as well as its methylation and imprinting status. Therefore, the aim of this study was to analyze the polymorphism of the coding region of the bovine IGF2 gene and to determine the sequence of its gene exon 6 in Bos taurus and Bos indicus cattle. A single nucleotide "C" deletion/insertion polymorphism was found in both cattle subspecies and a G/T transversion (RFLP-MboII) in the Bos indicus IGF2 gene. A 407-bp fragment of bovine IGF2 exon 6 was sequenced and the sequences (including variable nucleotides) were deposited in the GenBank database. A comparative analysis was performed for this fragment from different species; 99.5% identity was found between Bos taurus and Bos indicus cattle.
authors.affiliation: Institute of Genetics and Animal Breeding, Jastrzebiec, 05-552 Wólka Kosowska, Poland. krzysztof.flisikowski@gmail.com
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Flisikowski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Maj
authors.forename: Lech
authors.initials: L
authors.lastname: Zwierzchowski
authors.forename: Tatiana
authors.initials: T
authors.lastname: Adamowicz
authors.forename: Marek
authors.initials: M
authors.lastname: Switoński
authors.forename: Stefan
authors.initials: S
authors.lastname: Hiendleder
authors.forename: Chandra
authors.initials: C
authors.lastname: Pareek
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005091:Exons / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007335:Insulin-Like Growth Factor II; D004247:DNA