title: Early loss of arteriolar smooth muscle cells: more than just a pericyte loss in diabetic retinopathy.
abstract: Incipient diabetic retinopathy is characterized by increased capillary permeability and progressive capillary occlusion. The earliest structural change is the loss of pericytes (PC) from the retinal capillaries. With the availability of the XLacZ mouse, which expresses the LacZ reporter in a PC/vascular smooth muscle cell (vSMC) specific fashion, we quantitatively assessed the temporal dynamics of smooth muscle cells in arterioles under hyperglycemic conditions. We induced stable hyperglycemia in XLacZ mice. After 4, 8, and 12 weeks of diabetes retinae were isolated and beta-galactosidase/lectin stained. The numbers of smooth muscle cells were counted in retinal whole mounts, and diameters of retinal radial and branching arterioles and venules were analyzed at different distances apart from the center of the retina. After eight weeks of diabetes, the numbers of vSMCs were significantly reduced in radial arterioles 1000 microm distant from the optic disc. At proximal sites of branching arterioles (400 microm distant from the center), and at distal sites (1000 microm), vSMC were significantly reduced already after 4 weeks (to a maximum of 31 %). These changes were not associated with any measurable variation in vessel diameters. These data indicate quantitatively that hyperglycemia not only causes pericyte loss, but also loss of vSMCs in the retinal vasculature. Our data suggest that arteriolar vSMC in the eye underlie similar regulations which induce early pericyte loss in the diabetic retina.
authors.affiliation: 5th Medical Clinic, Faculty of Medicine Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer, 68167 Mannheim, Germany. Franziska.vomhagen@med5.ma.uni-heidelberg.de
authors.forename: F
authors.initials: F
authors.lastname: vom Hagen
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
authors.forename: A
authors.initials: A
authors.lastname: Hillenbrand
authors.forename: S
authors.initials: S
authors.lastname: Hoffmann
authors.forename: M
authors.initials: M
authors.lastname: Shani
authors.forename: U
authors.initials: U
authors.lastname: Deutsch
authors.forename: H P
authors.initials: HP
authors.lastname: Hammes
mesh_terms: D000818:Animals; D001160:Arterioles / Q000473:pathology*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000150:complications / Q000473:pathology; D003930:Diabetic Retinopathy / Q000150:complications / Q000473:pathology*; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D032389:Myocytes, Smooth Muscle / Q000473:pathology*; D013997:Time Factors