title: Molecular changes in PDEGF and bFGF in malignant melanomas in relation to the stromal microenvironment.
abstract: BACKGROUND
  Aberrant expression of either growth factors or growth factor-receptors by stromal cells can be an important factor promoting the growth of solid tumours. It may also affect differentiation of malignant cells and support tumour spread. The aim of the present study was to investigate the hypothesis that basic-fibroblast growth factor (bFGF) and platelet-derived growth factor (PDEGF) may be involved in tumour-stromal microenvironment interactions in primary malignant melanomas.
  
  
  METHODS
  PDEGF and bFGF expression in malignant cells and surrounding stromal elements was assessed using indirect immunohistochemistry.
  
  
  RESULTS
  It was confirmed that PDEGF can be involved in the reciprocal interactions between tumour cells and stroma, including aberrant angiogenesis. Interestingly, bFGF was present both in malignant melanoma lesions and benign nevi accompanied by different intracellular localisation of the protein, suggesting its implication in regulation of nevus cell proliferation and maturation.
  
  
  CONCLUSIONS
  The present results suggest that bFGF and PDEGF participate in malignant melanoma progression.
authors.affiliation: Institute of Pathology & Laboratory of Molecular Pathology, Faculty of Medicine, Palacky University, Hnevotinska 3, 77515 Olomouc, Czech Republic. mfiuraskova@seznam.cz
authors.forename: Michala
authors.initials: M
authors.lastname: Fiuraskova
authors.forename: Svetlana
authors.initials: S
authors.lastname: Brychtova
authors.forename: Eva
authors.initials: E
authors.lastname: Sedlakova
authors.forename: Petr
authors.initials: P
authors.lastname: Benes
authors.forename: Bohumil
authors.initials: B
authors.lastname: Zalesak
authors.forename: Alice
authors.initials: A
authors.lastname: Hlobilkova
authors.forename: Martin
authors.initials: M
authors.lastname: Tichy
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Kolar
mesh_terms: D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis*; D006801:Humans; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology*; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D010982:Platelet-Derived Growth Factor; D016222:Fibroblast Growth Factor 2