title: The Trp64Arg beta3-adrenergic receptor amino-acid variant is not associated with overweight and type 2 diabetes mellitus in Polish population.
abstract: The Trp64Arg amino-acid variant of the beta3 adrenoreceptor gene may be associated with a genetic predisposition to human obesity and related disorders, including type 2 diabetes mellitus. This relationship has been reported in various ethnic groups, however it was not extensively studied in Polish population. Therefore, the aim of the study was to investigate the association of Trp64Arg polymorphism of the beta3 adrenergic receptor gene with overweight and type 2 diabetes mellitus in polish subjects. The Trp64Arg polymorphism was determined by PCR-based MspI restriction fragment length polymorphism (PCR-RFLP). The study population consisted of 358 subjects, among whom 200 were diagnosed as overweight (BMI > 27 kg/m (2)). Among overweight subjects 111 presented with type 2 diabetes mellitus and 89 with normal glucose metabolism. All study participants were unrelated Caucasians and inhabited the city of Lodz, Poland. The frequency of the Arg allele did not differ significantly between overweight and normal weight patients (13 % vs. 11 %, OR 1.17, CI 0.74 - 1.85). The same applied to overweight diabetic patients vs. overweight patients without diabetes mellitus (13 % vs. 13 %, OR 0.97, CI 0.54 - 1.76). The obtained results suggest no association between Trp64Arg polymorphism of the beta3-AR gene and the incidence of overweight and type 2 diabetes mellitus in Polish population.
authors.affiliation: Department of Clinical Pharmacology, Medical University of Lodz, Lodz, Poland. jkasznicki@up.pl
authors.forename: J
authors.initials: J
authors.lastname: Kasznicki
authors.forename: J
authors.initials: J
authors.lastname: Blasiak
authors.forename: I
authors.initials: I
authors.lastname: Majsterek
authors.forename: K
authors.initials: K
authors.lastname: Przybyłowska
authors.forename: J
authors.initials: J
authors.lastname: Drzewoski
mesh_terms: D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005838:Genotype; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008297:Male; D020125:Mutation, Missense / Q000235:genetics*; D050177:Overweight / Q000235:genetics*; D011044:Poland; D011110:Polymorphism, Genetic / Q000235:genetics; D022702:Receptors, Adrenergic, beta-3 / Q000737:chemistry / Q000235:genetics*; D014364:Tryptophan / Q000235:genetics*
chemical_list: D022702:Receptors, Adrenergic, beta-3; D014364:Tryptophan; D005947:Glucose