title: Detection and characterisation of a second potyvirus from Thunberg fritillary in China.
abstract: Plants of Thunberg fritillary (Fritillaria thunbergii Miq.) from Zhejiang Province, were found to be co-infected with two distinct potyviruses. One was an isolate of the recently reported Thunberg fritillary mosaic virus (TFMV; Wei et al., (2005) Arch Virol 150: 1271-1280), while the other was a distinct virus that did not react with TFMV antiserum nor with antisera to 17 other potyviruses, except for a weak reaction with antibodies produced to soybean mosaic virus (SMV) Pinellia strain. Both viruses could be transmitted mechanically to their original host but not to any of a range of commonly used indicator plants. No local lesion host was identified that would enable the viruses to be propagated independently. The complete sequences of both viruses were determined; that of the new virus (9656 nt) had the typical genome organisation and recognised sequence motifs of a potyvirus, encoding a putative polyprotein of 351 kDa. Phylogenetic analysis, sequence comparisons, and the pattern of polyprotein cleavage sites all indicated that it was a member of the Bean common mosaic virus subgroup. The most closely related species are Soybean mosaic virus and Wisteria vein mosaic virus, with 68-69% amino acid identity between their polyproteins. This is sufficiently different for the new virus to be regarded as a distinct species, which we have tentatively named Fritillary virus Y.
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, People's Republic of China. jchen1@mail.hz.zj.cn
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: H-Y
authors.initials: HY
authors.lastname: Zheng
authors.forename: Y-H
authors.initials: YH
authors.lastname: Shi
authors.forename: M J
authors.initials: MJ
authors.lastname: Adams
authors.forename: C-B
authors.initials: CB
authors.lastname: Wei
authors.forename: L
authors.initials: L
authors.lastname: Lin
authors.forename: J-P
authors.initials: JP
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D002681:China; D004279:DNA, Viral / Q000235:genetics; D031410:Fritillaria / Q000821:virology*; D016679:Genome, Viral; D008854:Microscopy, Electron; D010802:Phylogeny; D017800:Potyvirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral