title: Regulation of the inflammatory response to Staphylococcus aureus-induced brain abscess by interleukin-10.
abstract: A characteristic of brain abscess is a localized suppurative infection leading to substantial damage of the adjacent central nervous system tissue. The orchestrated interplay of pro- and antiinflammatory cytokines released by leukocytes as well as resident cells of the central nervous system is crucial for both an effective host defense and for limiting tissue damage in brain abscess. To study the regulatory role of interleukin (IL)-10 in brain abscess in vivo, IL-10-deficient (IL-10(0/0)) mice were stereotaxically infected with Staphylococcus aureus-laden agarose beads. Increased numbers of intracerebral (IC) granulocytes, macrophages, CD4+ and CD8+ T cells, and higher levels of TNF, IL-1beta, and iNOS were observed in IL-10(0/0) mice than in wild-type mice, whereas chemokines were induced earlier and more pronounced in wild-type mice. Together with prominent microvascular hemorrhage, necrotic vasculitis, severe brain edema, and markedly increased abscess size, these alterations led to an increased morbidity of IL-10(0/0) mice. Nevertheless, the hyperinflammatory response of IL-10(0/0) mice did not improve bacterial elimination. Collectively, these data outline the important role of IL-10 in vivo for the regulation of the IC host immune response in experimental S. aureus-induced brain abscess.
authors.affiliation: Abteilung für Neuropathologie, Universität zu Köln, Köln, Germany. w.stenzel@uni-koeln.de
authors.forename: Werner
authors.initials: W
authors.lastname: Stenzel
authors.forename: Julia
authors.initials: J
authors.lastname: Dahm
authors.forename: Monica
authors.initials: M
authors.lastname: Sanchez-Ruiz
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Miletic
authors.forename: Manuel
authors.initials: M
authors.lastname: Hermann
authors.forename: Cornelius
authors.initials: C
authors.lastname: Courts
authors.forename: Heinrich
authors.initials: H
authors.lastname: Schwindt
authors.forename: Olaf
authors.initials: O
authors.lastname: Utermöhlen
authors.forename: Dirk
authors.initials: D
authors.lastname: Schlüter
authors.forename: Martina
authors.initials: M
authors.lastname: Deckert
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D001922:Brain Abscess / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D018925:Chemokines / Q000235:genetics; D015169:Colony Count, Microbial; D003199:Computer Systems; D016207:Cytokines / Q000235:genetics; D004660:Encephalitis / Q000382:microbiology*; D016753:Interleukin-10 / Q000378:metabolism*; D007700:Kinetics; D007962:Leukocytes / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D013203:Staphylococcal Infections / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D013211:Staphylococcus aureus
chemical_list: D018925:Chemokines; D016207:Cytokines; D012333:RNA, Messenger; D016753:Interleukin-10