title: [Angiogenesis promoting effects of Chinese herbal medicine for activating blood circulation to remove stasis on chick embryo chorio-allantoic membrane].
abstract: OBJECTIVE
  To observe the angiogenesis promoting effects of clinical common used Chinese herbal medicines (CHM) for activating blood circulation to remove stasis on chick embryo chorio-allantoic membrane (CAM).
  
  
  METHODS
  Chicken CAM model was established and mice blood serum containing different kinds of medicines, including Radix Peaoniae rubra, Radix Angelicae sinensis, Flos Carthami, Rhizoma Chuanxiong, Radix Salviae miltiorrhizae, Astragalus membranaceus, and their complex prescriptions, Danggui Buxue Decoction, Xuefu Zhuyu Decoction, Xiongshao Capsule, was applied on it respectively to observe the condition of angiogenesis 72 hrs after incubation. Besides, the normal saline group, blank serum group, blank group and basic fibroblast growth factor (bFGF) group were set up for control.
  
  
  RESULTS
  All the CHM applied and bFGF had the CAM angiogenetic promoting effect, among them, Radix Salviae Miltiorrhizae and the three complex prescriptions showed better effects than the three negative control groups in capillary formation and count, with the efficacy similar to that of bFGF. The effect of complex prescriptions was superior to that of single herb except Radix Salviae miltiorrhizae.
  
  
  CONCLUSIONS
  Radix Salviae miltiorrhizae, Danggui Buxue Decoction, Xuefu Zhuyu Decoction and Xiongshao Capsule have good angiogenesis promoting effect on CAM. This study elucidated, from a certain aspect, the mechanism of action of CHM on ischemic diseases, and unfolded the scientific evidence of applying complex prescription.
authors.affiliation: Fujian College of Traditional Chinese Medicine, Fuzho 250003.
authors.forename: Dong
authors.initials: D
authors.lastname: Gao
authors.forename: Jun
authors.initials: J
authors.lastname: Song
authors.forename: Juan
authors.initials: J
authors.lastname: Hu
authors.forename: Jiumao
authors.initials: J
authors.lastname: Lin
authors.forename: Liangpu
authors.initials: L
authors.lastname: Zheng
authors.forename: Jing
authors.initials: J
authors.lastname: Cai
authors.forename: Jian
authors.initials: J
authors.lastname: Du
authors.forename: Keji
authors.initials: K
authors.lastname: Chen
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000818:Animals; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D002823:Chorion / Q000098:blood supply; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011897:Random Allocation; D027561:Salvia miltiorrhiza
chemical_list: D043925:Angiogenesis Inducing Agents; D004365:Drugs, Chinese Herbal; C513826:Xue-Fu-Zhu-Yu decoction; C477279:danggui buxue decoction