The vaccine potential of Streptococcus pneumoniae surface lectin- and non-lectin proteins.
We have fractionated S. pneumoniae surface proteins into lectin and non-lectin fractions and tested their ability to elicit protective immune responses in the mouse model system. The total cell wall protein fraction (CW-T) was separated into lectin (CW-L), and non-lectin (CW-NL) fractions and used for immunization of mice. Immunized mice were challenged intranasally or intraperitoneally with S. pneumoniae strain WU2 (serotype 3). CW-T, CW-NL and CW-L and adjuvant only vaccination protected 55%, 43%, 44% and 0% of the intranasally challenged mice, respectively and 67%, 86%, 11% and 0% of mice challenged intraperitoneally, respectively. Immunogenic proteins in each fraction were sequenced and identified using MALDI-TOF. CW-L proteins provided a significantly better protection against intranasal inoculation and CW-NL proteins provided a significantly better protection from intraperitoneal inoculation. Proteins identified by sera from mice immunized with the cell-wall derived fractions may constitute candidates for future development of anti S. pneumoniae vaccines.
Portnoi
Maxim
M
Pediatric Infectious Disease Unit, Soroka University Medical Center, Beer Sheva, Israel.
Ling
Eduard
E
Feldman
Galia
G
Dagan
Ron
R
Mizrachi-Nebenzahl
Yaffa
Y
D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D002473:Cell Wall / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D037102:Lectins / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D008565:Membrane Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011008:Pneumococcal Infections / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000276:immunology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013296:Streptococcus pneumoniae / Q000276:immunology*; D016019:Survival Analysis
D001426:Bacterial Proteins; D037102:Lectins; D008565:Membrane Proteins; D022242:Pneumococcal Vaccines