Methyl oleate modulates LIP2 expression in the lipolytic yeast Yarrowia lipolytica.
Methyl oleate was used as a primary carbon source and as an alternative inducer for the production of an extracellular lipase, Lip2, in Y. lipolytica strain LgX64.81 grown in a 20-l bioreactor. The lipase-encoding gene, LIP2, was investigated during culture on methyl oleate using a pLIP2-LacZ reporter fusion and we provide evidence for the involvement of methyl oleate in its regulation.
Fickers
Patrick
P
Centre Wallon de Biologie Industrielle, Service de Technologie Microbienne, Université de Liège, Bd du Rectorat Bat. 40, B-4000, Liège, Belgium. p.thonart@ulg.ac.be
Destain
Jacqueline
J
Thonart
Philippe
P
D001426:Bacterial Proteins / Q000096:biosynthesis*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005656:Fungal Proteins; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000502:physiology*; D008049:Lipase / Q000096:biosynthesis*; D008066:Lipolysis / Q000187:drug effects / Q000502:physiology*; D009829:Oleic Acids / Q000008:administration & dosage*; D025062:Yarrowia / Q000187:drug effects / Q000502:physiology*
D001426:Bacterial Proteins; D005656:Fungal Proteins; D009829:Oleic Acids; C005576:methyl oleate; C408971:LIP2 protein, Yarrowia lipolytica; D008049:Lipase