title: PCR protocol for specific identification of Candida nivariensis, a recently described pathogenic yeast.
abstract: Candida nivariensis is a recently described pathogenic yeast closely related to Candida glabrata. We developed a specific set of oligonucleotide primers based on the internal transcribed spacer regions of the rRNA gene for the rapid identification of C. nivariensis. PCR with these primers amplified a 206-bp amplicon from C. nivariensis.
authors.affiliation: Unidad de Investigación, Hospital Universitario Nuestra Señora de Candelaria, Ctra. del Rosario s/n, 38010 Santa Cruz de Tenerife, Canary Islands, Spain.
authors.forename: Julia
authors.initials: J
authors.lastname: Alcoba-Flórez
authors.forename: María del Pilar
authors.initials: Mdel P
authors.lastname: Arévalo
authors.forename: F Javier
authors.initials: FJ
authors.lastname: González-Paredes
authors.forename: Josep
authors.initials: J
authors.lastname: Cano
authors.forename: Josep
authors.initials: J
authors.lastname: Guarro
authors.forename: Eduardo
authors.initials: E
authors.lastname: Pérez-Roth
authors.forename: Sebastián
authors.initials: S
authors.lastname: Méndez-Alvarez
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D002177:Candidiasis / Q000382:microbiology*; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D006801:Humans; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal