[Effect of indomethacin on fibronectin and plasminogen activator inhibitor-1 in cultured human peritoneal mesothelial cells].
OBJECTIVE
To determine the effect of indomethacin on high concentration glucose and lipopolysaccharide (LPS) induced fibronectin (FN) and plasminogen activator inhibitor-1 (PAI-1) secretion in cultured human peritoneal mesothelial cells.


METHODS
Mesothelial cells were isolated from human omental specimens by trypsin disaggregation and incubated by 2.5% glucose or LPS together with different concentrations of indomethacin. Enzyme-linked immunosorbent assay determined the quantity of FN and PAI-1 in the cultured supernatants.


RESULTS
Compared with the control group, the levels of FN and PAI-1 in the cultured supernatants were increased significantly exposuring to high concentration glucose and LPS (P <0.01). The different concentrations of indomethacin decreased FN and PAI-1 secretion in the 2.5% glucose or the LPS group within 24 h (P < 0.05).


CONCLUSIONS
Indomethacin can inhibit the synthesis and secretion of extracellular matrix in human peritoneal mesothelial cells, which may be effective in the gene therapy for peritoneal fibrosis.
Liu
Xin
X
Department of Nephrology, First Hospital, Tsinghua Universiiy, Beijing, China. felh@medmail.com.cn
Peng
You-Ming
YM
Liu
Fu-You
FY
Wei
Jia-Li
JL
D002478:Cells, Cultured; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D010537:Peritoneum / Q000166:cytology / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism*
D016861:Cyclooxygenase Inhibitors; D005353:Fibronectins; D017395:Plasminogen Activator Inhibitor 1; D007213:Indomethacin