Propionicicella superfundia gen. nov., sp. nov., a chlorosolvent-tolerant propionate-forming, facultative anaerobic bacterium isolated from contaminated groundwater.
A novel strain, designated as BL-10(T), was characterized using a polyphasic approach after isolation from groundwater contaminated by a mixture of chlorosolvents that included 1,1,2-trichloroethane, 1,2-dichloroethane, and vinyl chloride. Stain BL-10(T) is a facultatively anaerobic bacterium able to ferment glucose to form propionate, acetate, formate, lactate, and succinate. Fermentation occurred in the presence of 1,2-dichloroethane and 1,1,2-trichloroethane at concentrations to at least 9.8 and 5.9 mM, respectively. Cells are Gram-positive, rod-shaped, non-motile, and do not form spores. Oxidase and catalase are not produced and nitrate reduction did not occur in PYG medium. Menaquinone MK-9 is the predominant respiratory quinone and meso-diaminopimelic acid is present in the cell wall peptidoglycan layer. Major cellular fatty acids are C(15:0), iso C(16:0), and anteiso C(15:0). Genomic DNA G + C content is 69.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed strain BL-10(T) to fall within the radiation of genera Propionicimonas and Micropruina. On the basis of the results obtained in this study, it is proposed that strain BL-10(T) should be classified as a novel taxon, for which the name Propionicicella superfundia gen. nov., sp. nov. is proposed. The type strain of Propionicicella superfundia is BL-10(T) (=ATCC BAA-1218(T), =LMG 23096(T)).
Bae
Hee-Sung
HS
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Moe
William M
WM
Yan
Jun
J
Tiago
Igor
I
da Costa
Milton S
MS
Rainey
Fred A
FA
D001673:Biodegradation, Environmental; D005025:Ethylene Dichlorides / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology; D006092:Gram-Negative Facultatively Anaerobic Rods / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D014240:Trichloroethanes / Q000378:metabolism; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism
D005025:Ethylene Dichlorides; D012336:RNA, Ribosomal, 16S; D014240:Trichloroethanes; D014874:Water Pollutants, Chemical; C024567:1,1,2-trichloroethane; C024565:ethylene dichloride