[Characteristics and expression of Mip5, a novel gene associated with myocardial ischemia/reperfusion in rats].
OBJECTIVE
To determine the characteristics of a novel gene Mip5 (GenBank accession number AY553870) and its expression under physiological and pathological conditions.


METHODS
The characteristics of Mip5 were analyzed by bioinformatic programs including BLAST, spidey, psort, ClustalW and so on. RT-PCR was performed to detect Mip5 expression.


RESULTS
Bioinformatic analysis showed that Mip5 gene lied in the 13th chromosome and contained 8 exons and 7 introns, its open reading frame contained 909 bp and its protein production was 302 amino acid residues including 6 kelth domains. Under normal conditions, MIP5 expressed abundantly in the heart, brain and kidney, but its expression could not be detected in the liver and muscle. Expression of Mip5 gene was increased significantly after ischemia-reperfusion compared with the sham groups, and reached its peak at 3 h and recovered at 12 h after the reperfusion. Conclusion Mip5 gene is a novel gene containing a putative open reading frame of 302 amino acids residues and may play an important role in rat cardiomyocytes suffering ischemia processing.
Wang
Jian-She
JS
Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China. xianzhongxiao@hotmail.com
Yuan
Can
C
Wang
Kang-Kai
KK
Zhang
Hua-Li
HL
E
Shun-Mei
SM
Liu
Mei-Dong
MD
Liu
Ke
K
Chen
Guang-Wen
GW
Xiao
Xian-Zhong
XZ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D017202:Myocardial Ischemia / Q000235:genetics*; D015428:Myocardial Reperfusion Injury / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics*; D051381:Rats
D018076:DNA, Complementary