[Blockage of allergic rhinitis in mice with recombinant protein of cytotoxic T lymphocyte associated antigen-4 extracellular domain].
OBJECTIVE
To explore a new immunotherapy against allergic rhinitis.


METHODS
The recombinant protein of CTLA4 extracellular domain was obtained through construction of CTLA4-yeast expression system. The allergic rhinitis in mice was induced by sensitizing and challenging with ovalbumin (OVA). The allergic rhinitis related symptoms and the morphological changes in nasal mucosa were compared between the allergic rhinitis group and the CTLA4 extracellular domain group treated with CTLA4 extracellular domain before each challenge by ways of intraperitoneal injection.


RESULTS
CTLA4 extracellular domain with a molecular weight of 28 000, which was confirmed by Western blot, could be generated through CTLA4-yeast expression system. The purified CTLA4 extracellular domain could inhibit T cells proliferation in mixed lymphocyte reaction with a inhibitory rate of 95.4%. The mice in allergic rhinitis group appeared typical allergic rhinitis symptoms after OVA challenge, such as rhinorrhea and sneeze. Meanwhile the nasal pathological studies showed edema and congestion in mucosa tissue and local influx of inflammatory cells. Whereas in CTLA4 extracellular domain group, the nasal symptoms were rarely observed, and the pathological change in nasal mucosa was significantly abated.


CONCLUSIONS
The protein of CTLA4 extracellular domain could prevent the allergic rhinitis in mice. The underlying mechanism of which might be the inhibition of the T cell activation.
Zhu
Jin
J
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tong Ren Hospital Affiliated to Capital University of Medical Sciences, Beijing 100730, China. zhujok@yahoo.com.cn
Han
De-Min
DM
Wu
Jun
J
He
Wei-Feng
WF
Chen
Xi-Wei
XW
Yi
Shao-Xuan
SX
D000818:Animals; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D060908:CTLA-4 Antigen; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000473:pathology*; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*
D015703:Antigens, CD; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; C556707:Ctla4 protein, mouse; D011994:Recombinant Proteins; D010047:Ovalbumin