[Study on using in vitro 3T3 neutral red uptake to test phototoxicity].
OBJECTIVE
To establish neutral red uptake test of 3T3 in vitro to detect phototoxicity of chemicals and study the in vitro 3T3 NUR as an alternative method instead of animal skin phototoxicity test.


METHODS
Using incubated Balb/c3T3 cells to establish the optimal conditions of reaction system.


RESULTS
The concentration of neutral red, the incubate time, pH and the density of cells were the factors effecting test results.


CONCLUSIONS
It is suggested that in vitro 3T3 neutral red uptake test can be used as an alternative instead of animals phototoxicity test.
Zhang
Hong-Wei
HW
Institute for Environment and Product Related Health Safety, China Center for Disease Control and Prevention, Beijing 100021, China.
Dong
Shao-Xia
SX
D000818:Animals; D041702:BALB 3T3 Cells; D003358:Cosmetics / Q000633:toxicity*; D017484:Dermatitis, Phototoxic; D007202:Indicators and Reagents / Q000378:metabolism*; D008730:Methoxsalen / Q000633:toxicity; D051379:Mice; D009499:Neutral Red / Q000378:metabolism*; D018675:Toxicity Tests / Q000379:methods*
D003358:Cosmetics; D007202:Indicators and Reagents; D009499:Neutral Red; D008730:Methoxsalen