title: Convenient and rapid analysis of linkage isomers of fucose-containing oligosaccharides by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry.
abstract: Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) was used to analyze three pyridylamino (PA)-fucosyloligosaccharides isolated from human milk: lacto-N-fucopentaose (LNFP) I [Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-PA], LNFP II [Galbeta1-3(Fucalpha1-4)GlcNAcbeta1-3Galbeta1-4Glc-PA], and LNFP III [Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc-PA]. These oligosaccharides are linkage isomers. MALDI-QIT-TOF MS provides MS(n) spectra, which we used to characterize these PA-oligosaccharides. MS/MS/MS analysis of the non-reducing end tri-saccharide ions generated by MS/MS was able to distinguish these oligosaccharide isomers. The MALDI-QIT-TOF MS is a very convenient and rapid method, therefore, it would be useful for high throughput structural analyses of various types of pyridylaminated oligosaccharide isomers.
authors.affiliation: Sphingolipid Expression Laboratory, Supra Biomolecular System Research Group, RIKEN Frontier Research System, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Minoru
authors.initials: M
authors.lastname: Suzuki
authors.forename: Emi
authors.initials: E
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishii
authors.forename: Kouzou
authors.initials: K
authors.lastname: Miseki
authors.forename: Akemi
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D002240:Carbohydrate Sequence; D005260:Female; D005643:Fucose / Q000032:analysis*; D006801:Humans; D007536:Isomerism; D008895:Milk, Human / Q000737:chemistry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D025942:Oligosaccharides, Branched-Chain / Q000032:analysis / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009844:Oligosaccharides; D025942:Oligosaccharides, Branched-Chain; D011725:Pyridines; D005643:Fucose