title: A role for LEDGF/p75 in targeting HIV DNA integration.
abstract: HIV DNA integration is favored in active genes, but the underlying mechanism is unclear. Cellular lens epithelium-derived growth factor (LEDGF/p75) binds both chromosomal DNA and HIV integrase, and might therefore direct integration by a tethering interaction. We analyzed HIV integration in cells depleted for LEDGF/p75, and found that integration was (i) less frequent in transcription units, (ii) less frequent in genes regulated by LEDGF/p75 and (iii) more frequent in GC-rich DNA. LEDGF is thus the first example of a cellular protein controlling the location of HIV integration in human cells.
authors.affiliation: University of Pennsylvania School of Medicine, Department of Microbiology, 3610 Hamilton Walk, Philadelphia, Pennsylvania 19104-6076, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Ciuffi
authors.forename: Manuel
authors.initials: M
authors.lastname: Llano
authors.forename: Eric
authors.initials: E
authors.lastname: Poeschla
authors.forename: Christian
authors.initials: C
authors.lastname: Hoffmann
authors.forename: Jeremy
authors.initials: J
authors.lastname: Leipzig
authors.forename: Paul
authors.initials: P
authors.lastname: Shinn
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Ecker
authors.forename: Frederic
authors.initials: F
authors.lastname: Bushman
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D020868:Gene Silencing; D006678:HIV / Q000235:genetics* / Q000502:physiology; D019427:HIV Integrase / Q000378:metabolism; D006801:Humans; D046228:Microarray Analysis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016662:Virus Integration / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C504649:PSIP1 protein, human; D014157:Transcription Factors; D004247:DNA; D019427:HIV Integrase