title: Response of osteoblastic cells to titanium submitted to three different surface treatments.
abstract: In the complex process of bone formation at the implant-tissue interface, surface properties are relevant factors modulating osteoblastic function. In this study, commercially pure titanium (cp Ti) samples were prepared with different surface characteristics using chemical attack with a sulfuric acid/hydrochloric acid based solution (treatment A); chemical attack plus anodic oxidation using phosphoric acid (treatment B); and chemical attack plus thermal oxidation followed by immersion in a sodium fluoride solution (treatment C). The samples were characterized by scanning electron microscopy (SEM), contact profilometry and contact angle. The biological performance of the prepared surfaces was evaluated using mice osteoblastic cell cultures for up to 21 days. Cells seeded on the different titanium samples showed similar behavior during cell attachment and spreading. However, cellular proliferation and differentiation were higher for samples submitted to treatments A and C (p < or = 0.05; n = 3), which were less rough and showed surface free energy with smaller polar components.
authors.affiliation: Metallurgical and Materials Department, Alberto Luiz Coimbra Graduate Studies and Engineering Research Institute, Federal University of Rio de Janeiro.
authors.forename: Adriana Soares
authors.initials: AS
authors.lastname: Santiago
authors.forename: Euler Araújo dos
authors.initials: EA
authors.lastname: Santos
authors.forename: Márcia Soares
authors.initials: MS
authors.lastname: Sader
authors.forename: Marcelo Felippe
authors.initials: MF
authors.lastname: Santiago
authors.forename: Gloria de Almeida
authors.initials: Gde A
authors.lastname: Soares
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D003764:Dental Materials / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000494:pharmacology; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D010084:Oxidation-Reduction; D010756:Phosphoric Acids / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology; D013464:Sulfuric Acids / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D003764:Dental Materials; D010756:Phosphoric Acids; D013464:Sulfuric Acids; D012969:Sodium Fluoride; D014025:Titanium; C030242:phosphoric acid; C033158:sulfuric acid; D006851:Hydrochloric Acid