Molecular cloning of rHAUSP encoding a deubiquitinating enzyme in rat testis.
The tumor suppressor protein p53 is stabilized by the herpes-virus-associated ubiquitin-specific protease (HAUSP), a deubiquitinating enzyme. We previously isolated and characterized a mouse orthologue of HAUSP, mHAUSP. In this study, we have identified a rat orthologue of HAUSP, rHAUSP, from the rat testis by RT-PCR using primers used for cloning mHAUSP. rHAUSP cDNA encodes 3,312 bp and 1,103 amino acids with a molecular weight of approximately 135 kDa containing highly conserved Cys, Asp (I), His, and Asn/Asp (II) domains characteristic of the ubiquitin-specific processing proteases. pI value of rHAUSP is 5.31. In vivo and in vitro deubiquitinating enzyme assays demonstrated that rHAUSP has deubiquitinating enzymatic activity. The over-expression of rHAUSP induced cell death of cervical adenocarcinoma cells.
Baek
Kwang-Hyun
KH
Graduate School of Life Science and Biotechnology, Pochon CHA University, Korea. baek@cha.ac.kr
Lee
Hye-Jin
HJ
Kim
Myung-Sun
MS
Kim
Yong-Soo
YS
Seong
Minu
M
Lee
Eung-Ji
EJ
Lee
Min-Young
MY
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D003001:Cloning, Molecular; D053938:DNA Fragmentation / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D013737:Testis / Q000201:enzymology*; D014162:Transfection; D025801:Ubiquitin / Q000378:metabolism; D000076084:Ubiquitin-Specific Peptidase 7
D018076:DNA, Complementary; D025801:Ubiquitin; D010450:Endopeptidases; D000076084:Ubiquitin-Specific Peptidase 7; C504243:Usp7 protein, rat