High-performance liquid chromatographic analysis of bisphenol A and 4-nonylphenol in serum, liver and testis tissues after oral administration to rats and its application to toxicokinetic study.
A sensitive and simple method based on solid-phase extraction (SPE) and HPLC with fluorescence detection for the determination of bisphenol A (BPA) and 4-nonylphenol (4-NP) in rat serum, liver and testis tissues has been developed. The chromatographic conditions consisted of a C18 column and mobile phase composition of acetonitrile and water with flow rate of 1.0 ml/min. The fluorescence detection was performed at excitation and emission wavelengths of 227 nm and 313 nm, respectively. Under these conditions, BPA and 4-NP were well separated and showed good linearities in the ranges of 0.01-50.0 microg/ml for BPA and 0.15-150.0 microg/ml for 4-NP with correlation coefficients greater than 0.999. The detection limits of serum and tissue samples were 2.8 ng/ml and 1.4 ng/g for BPA and 5.6 ng/ml and 2.8 ng/g for 4-NP at a signal-to-noise ratio (S/N) of 3. The intra-assay and the inter-assay precisions were better than 11.4%. Recoveries of BPA and 4-NP were 78.6-95.0% and 80.2-93.4%, respectively. The proposed method was applied to a toxicokinetic study of BPA and 4-NP including individual and combined oral administration to rats. The results showed that 4-NP remarkably altered the toxicokinetic parameters of BPA in testis, while parameters of BPA were not obviously altered in serum and liver under the experimental conditions investigated. On the other hand, there was no significant difference in the toxicokinetics of 4-NP when administered with BPA.
Xiao
Quanwei
Q
West China School of Public Health, Sichuan University, Chengdu, Sichuan 610041, China.
Li
Yuanqian
Y
Ouyang
Huaxue
H
Xu
Peiyu
P
Wu
Desheng
D
D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004347:Drug Interactions; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008657:Metabolic Clearance Rate; D010636:Phenols / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013737:Testis / Q000737:chemistry* / Q000378:metabolism; D014018:Tissue Distribution; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D010636:Phenols; C041594:4-nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds