Protective effects of flavonols and their glycosides against free radical-induced oxidative hemolysis of red blood cells.
The in vitro oxidative hemolysis of human red blood cells (RBCs) was used as a model to study the free radical-induced damage of biological membranes and the protective effect of flavonols and their glycosides (FOHs), i.e., myricetin (MY), quercetin (Q), morin (MO), kaempferol (K), rutin (R), quercetin galactopyranoside (QG), quercetin rhamnopyranoside (QR), and kaempferol glucopyranoside (KG). The hemolysis of RBCs was induced by a water-soluble free radical initiator 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AAPH). It was found that addition of AAPH at 37 degrees C to the suspension of RBCs caused fast hemolysis after a short period of inhibition period, and addition of FOHs significantly suppressed the hemolysis. The FOHs (MY, Q, R, QG and QR) which bears an ortho-dihydroxyl functionality showed much more effective anti-hemolysis activity than that of the other FOHs (MO, K and KG) bearing no such functionality.
Dai
Fang
F
National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, Gansu 730000, China.
Miao
Qing
Q
Zhou
Bo
B
Yang
Li
L
Liu
Zhong-Li
ZL
D000578:Amidines / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects*; D044948:Flavonols / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D024502:alpha-Tocopherol / Q000494:pharmacology
D000578:Amidines; D000975:Antioxidants; D044948:Flavonols; D016166:Free Radical Scavengers; D005609:Free Radicals; D006027:Glycosides; C046728:2,2'-azobis(2-amidinopropane); D024502:alpha-Tocopherol