Cloning and expression of NAD+-dependent L-arabinitol 4-dehydrogenase gene (ladA) of Aspergillus oryzae.
A gene of Aspergillus oryzae, ladA, which encodes L-arabinitol 4-dehydrogenase (EC 1.1.1.12), and its cDNA were cloned in Escherichia coli. The gene consisted of a 1209-bp coding region, interrupted by a 59-bp intron, which encoded a 382-amino-acid polypeptide (40,812 Da). The protein showed 67% identity to a well-studied L-arabinitol 4-dehydrogenase (Lad1) of Hypocrea jecorina. The cell-free extract of E. coli, which expressed ladA cDNA, showed L-arabinitol dehydrogenase activity with NAD+. It was also reactive for ribitol and xylitol.
Suzuki
Tohru
T
Life Science Research Center, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. suzuki@cc.gifu-u.ac.jp
Tran
Lien Ha
LH
Yogo
Masanori
M
Idota
Osamu
O
Kitamoto
Noriyuki
N
Kawai
Keiichi
K
Takamizawa
Kazuhiro
K
D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005091:Exons / Q000235:genetics; D005800:Genes, Fungal; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism*; D016415:Sequence Alignment; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics* / Q000378:metabolism*
D009243:NAD; D013401:Sugar Alcohol Dehydrogenases; C440029:L-arabinitol 4-dehydrogenase