[Efficacy of Hushen Gujing (HSGJ) in preventing chronic allograft nephropathy in rats].
OBJECTIVE
To evaluate the effect of HSGJ on chronic allograft nephropathy (CAN) using standard rat model of CAN.


METHODS
Renal transplantation was performed with Fisher rats as donors and Lewis rats as recipients. All the recipients were randomly divided into control group and medication groups (high and low dosage of HSGJ, fed every other day). After 16 weeks of treatment, renal function and the histological alteration of CAN were measured. The expression of the TGFbeta1 mRNA in the allograft was evaluated by real-time PCR.


RESULTS
The content of 24 h urine protein and the level of serum creatinine in the medication groups were significantly decreased (P < 0.01) as compared with control group, whereas the creatinine clearance was increased (P < 0.01). The degree of glomerular sclerosis and the Banff score of medication groups were lower than the control group respectively (P < 0.01), in consistent with decreased expression of the TGF 1mRNA.


CONCLUSIONS
HSGJ can prevent the chronic allograft nephropathy and the mechanism may be related with its influence on the expression of the TGFbeta1.
Tian
Xiao-hui
XH
The Department of Renal Transplantation, The First Hospital of Xi'An JiaoTong University, Xi'an 710061, China.
Xue
Wu-jun
WJ
Wang
Zhi-yong
ZY
Zhou
Qing-fa
QF
Zhang
Yin-sheng
YS
Ding
Xiao-ming
XM
Tian
Pu-xun
PX
D000818:Animals; D002908:Chronic Disease; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005921:Glomerulonephritis / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D006084:Graft Rejection / Q000188:drug therapy*; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D016030:Kidney Transplantation / Q000009:adverse effects*; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D014184:Transplantation, Homologous
D004365:Drugs, Chinese Herbal; D007166:Immunosuppressive Agents