[Intergration and epression of porcine endogenous retrovinus in the immortal cell line of Banna Minipig Inberd Line-Mesenhymal Stem Cells].
OBJECTIVE
To detect the integration and expression of porcine endogenous retrovirus (PERV) in the immortal cell line of Banna Minipig Inbred Line-Mesenchymal Stem Cells (BMI-MSCs).


METHODS
DNA and total RNA of the immortal cell line of BMI-MSCs were extracted and PCR, RT-PCR were performed to detect PERV-gag, pol and env gene, and the type of PERV was also detected.


RESULTS
PERV-gag, pol and env gene were all detected in the primary culture and immortal cell line (passage 150 and passage 180) of BMI-MSCs, and the type of PERV was PERV-A, B. Functional expression of PERV-gag and pol mRNA was also detected.


CONCLUSIONS
In this laboratory, PERV was not lost during the proceeding of pig inbred and since has been in long-term culture of pig cells in vitro. PERV has integrated into the genome of its natural host, and virus mRNA can effectively express. So it is very essential to evaluate the possibility of xenozoonoses in pig-to-human xenotransplantation.
Yu
Ping
P
Lab of Transplantation Engineering and Immunology, West China Hospital, Sichuan University, Chengdu 610041, China.
Liu
Jin
J
Zhang
Li
L
Li
Shrng-Fu
SF
Bu
Hong
H
Li
You-Ping
YP
Cheng
Jing-Qui
JQ
Lu
Yan-Rong
YR
Long
Dan
D
D000818:Animals; D017849:Animals, Inbred Strains; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002460:Cell Line; D020077:Endogenous Retroviruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015870:Gene Expression; D015683:Gene Products, gag / Q000096:biosynthesis / Q000235:genetics; D015684:Gene Products, pol / Q000096:biosynthesis / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D011995:Recombination, Genetic; D013552:Swine; D013556:Swine, Miniature / Q000821:virology*; D014183:Transplantation, Heterologous / Q000009:adverse effects; D016662:Virus Integration / Q000235:genetics*
D015683:Gene Products, gag; D015684:Gene Products, pol