title: Influence of different dietary fat intake on liver metastasis and hepatic lipid peroxidation in BOP-induced pancreatic cancer in Syrian hamsters.
abstract: OBJECTIVE
  Effects of polyunsaturated fatty acids (PUFA) on carcinogenesis are discussed controversially. Thus, tumor growth seems to be influenced by type and composition of fat dietary; however, the pathomechanism is still unknown. Therefore, we investigated the impact of different PUFAs on liver metastasis and hepatic lipid peroxidation in a solid model of ductal pancreatic cancer in Syrian hamsters.
  
  
  METHODS
  90 male hamsters were randomized into 6 groups (n = 15). Accordingly groups 2, 4 and 6 received 10 mg N-nitrosobis-2-oxopropylamine (BOP)/kg body weight weekly by subcutaneous injection for 12 weeks in order to induce ductal pancreatic cancer, while groups 1, 3 and 5 were treated with 0.5 ml 0.9% sodium chloride. All hamsters received a standard fat diet (SFD) rich in n-6 PUFA for 16 weeks (2.9% fat). Afterwards, groups 1 and 2 had free access to SFD, while groups 3 and 4 were given a diet enriched with n-3, n-6 and n-9 PUFA (SMOF) and groups 5 and 6 were fed a diet high in n-3 PUFA (FISH-OIL). After 32 weeks all hamsters were sacrificed in order to determine incidence of pancreatic carcinoma and liver metastasis. Furthermore hepatic activities of glutathionperoxidase (GSH-Px) and superoxiddismutase (SOD) as well as levels of lipidperoxidation were analyzed intra- and extrametastatically.
  
  
  RESULTS
  The incidence of liver metastasis was decreased in the FISH-OIL tumor group compared to the SFD and SMOF groups. However, GSH-Px activity was not influenced by different diets. Extrametastatic hepatic SOD activity did not differ between all groups, while intrametastatic hepatic SOD activity in the SFD-BOP group was increased. In the FISH-OIL-BOP and the SMOF-BOP group intrametastatic SOD activity was lower than in non-metastatic hepatic tissue. Furthermore levels of hepatic lipid peroxidation were decreased in the tumor groups treated with fish oil and SMOF compared to the SFD group. Comparing intra- and extrametastatic TBARS concentration there was no difference in the SFD-BOP and the SMOF-BOP groups, while in the FISH-OIL-BOP group intrametastatic TBARS concentration was increased.
  
  
  CONCLUSIONS
  Conclusively, fish oil reduced the incidence of liver metastasis in experimental ductal pancreatic cancer. Maybe this effect is caused by an increase of intrametastatic hepatic lipid peroxidation.
authors.affiliation: Clinic of General, Visceral, Vascular and Thoracic Surgery, Charité Campus Mitte, Humboldt-University of Berlin, Berlin, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Heukamp
authors.forename: J I
authors.initials: JI
authors.lastname: Gregor
authors.forename: M
authors.initials: M
authors.lastname: Kilian
authors.forename: C
authors.initials: C
authors.lastname: Kiewert
authors.forename: C A
authors.initials: CA
authors.lastname: Jacobi
authors.forename: I
authors.initials: I
authors.lastname: Schimke
authors.forename: M K
authors.initials: MK
authors.lastname: Walz
authors.forename: H
authors.initials: H
authors.lastname: Guski
authors.forename: F A
authors.initials: FA
authors.lastname: Wenger
mesh_terms: D000818:Animals; D002273:Carcinogens; D006224:Cricetinae; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary*; D008297:Male; D008647:Mesocricetus; D009602:Nitrosamines; D000069463:Olive Oil; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D010938:Plant Oils / Q000737:chemistry; D013024:Soybean Oil / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D002273:Carcinogens; D004041:Dietary Fats; D009602:Nitrosamines; D000069463:Olive Oil; D010938:Plant Oils; D017392:Thiobarbituric Acid Reactive Substances; C028133:N-nitrosomethyl-2-oxopropylamine; D013024:Soybean Oil; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase