Correlation between diverse cyclic lipopeptides production and regulation of growth and substrate utilization by Bacillus subtilis strains in a particular habitat.
The two Bacillus subtilis strains (DM-03 and DM-04) were isolated from two extremely different habitats; one from the traditional fermented food and another one from a petroleum contaminated soil sample. These strains produced quantitatively and qualitatively different cyclic lipopeptides isoforms under laboratory culture conditions. MALDI-TOF mass spectral analysis revealed that lipopeptide profile varied according to the producing B. subtilis strains; iturins and surfactins isoforms were pre-dominant cyclic lipopeptides produced by B. subtilis DM-03 and DM-04 strains, respectively. A comparative study showed that these strains possessed distinct preferences for the carbon and nitrogen substrates, temperature and pH for optimal growth and biosurfactant production. Our study documented that the cyclic lipopeptide isoforms produced by the respective strains played an important role in the utilization of available hydrophobic substrate(s) from their natural habitats and conferred some kind of competitive advantage to the producing B. subtilis strains in their parent ecological niche.
Mukherjee
Ashis K
AK
ONGC Centre for Petroleum Biotechnology, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784 028, India. akm@tezu.ernet.in
Das
Kishore
K
D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D018533:Biomass; D003470:Culture Media; D017931:DNA Primers; D004777:Environment; D004926:Escherichia coli / Q000187:drug effects; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D044086:Kluyvera / Q000187:drug effects; D008074:Lipoproteins / Q000378:metabolism* / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D012150:Polymorphism, Restriction Fragment Length; D012988:Soil Microbiology; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013501:Surface-Active Agents / Q000378:metabolism; D013696:Temperature; D015003:Yeasts / Q000187:drug effects
D003470:Culture Media; D017931:DNA Primers; D008074:Lipoproteins; D013501:Surface-Active Agents