Survival of pathogenic bacteria in compost with special reference to Escherichia coli.
Application of compost in agricultural practice could potentially cause contamination of foodstuffs with pathogenic bacteria such as Escherichia coli O157:H7 (E. Coli O157). We investigated pathogenic bacteria in compost collected from the compost facilities, and evaluated the survival of E. coli K12 and O157 in laboratory experiments. Out of 19 compost product samples, coliform bacteria and salmonella were detected in 7 and 3 samples respectively. The number of coliform bacteria was 1.8 x 10(2) to 2.5 x 10(6) CFU/g dw and that of salmonella was 4.2 x 10(1) to 6.0 x 10(3) CFU/g dw. Moreover, coliform bacteria, fecal coliform, E. coli and salmonella were detected during composting at 54 degrees C to 67 degrees C. The results indicated that moisture content was a very important factor to the heat sensitivity of pathogenic bacteria in compost, E. coil in compost of high moisture content was more sensitive than that in compost of low moisture content, cells harvested in logarithmic phase was more sensitive than these in stationary phase, and E. coli K12 was more sensitive than E. coli O157. Based on the D values, the lethal time of E. coli K12 and O157 from l0(8) to 10(0) CFU/g dw were 16.3 and 28.8 min, respectively, at 60 degrees C in compost with 40% moisture content. However, some E. coil cells survived in composting process at 54 degrees C to 67 degrees C. Water potential (low moisture content) and physiological aspects of bacteria (stationary phase) could explain only in part of the prolonged survival of E. coil in compost, and there should be some other factors that are conducive to bacterial survival in compost.
Gong
Chun-ming
CM
Laboratory of Soil Microbiology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan.
Koichi
Inoue
I
Shunji
Inanaga
I
Takashi
Someya
S
D004755:Enterobacteriaceae / Q000502:physiology; D004926:Escherichia coli / Q000502:physiology*; D006358:Hot Temperature; D007564:Japan; D012475:Salmonella / Q000502:physiology; D012987:Soil; D012988:Soil Microbiology; D013234:Stem Cells; D016019:Survival Analysis; D013997:Time Factors; D014867:Water / Q000032:analysis
D012987:Soil; D014867:Water