Glucocorticosteroid therapy decreases CD14-expression and CD14-mediated LPS-binding and activation of monocytes in patients suffering from systemic lupus erythematosus.
In order to study the possible action of glucocorticosteroids (GCS) on the CD14/Toll like receptor mediated activation of monocytes the CD14-expression, CD14-mediated LPS binding and activation of these cells of patients suffering from Systemic Lupus Erythematosus receiving no, low dose or pulse steroid treatment was studied. The CD14-expression was determined on whole blood monocytes by flow cytometry, while the LPS-binding of an FITC-LPS preparate and the LPS-induced TNFalpha secretion were tested on isolated monocytes. The CD14-dependent and -independent LPS-binding and activation were evaluated with the help of a blocking anti-CD14 mAb. Our results showed that the CD14-expression, CD14-dependent LPS-binding and activation were significantly inhibited by the in vivo applied pulse steroid therapy. In contrast, the CD14-independent LPS-binding and activation were not altered by the GCS treatment. Our data provide further in vivo evidence for a possible new way of GCS therapy is able to initiate its anti-inflammatory action.
Sümegi
Andrea
A
Department of Clinical Biochemistry and Molecular Pathology, Medical and Health Science Center, University of Debrecen, Nagyerdei str. 98., Debrecen-4012, Hungary. alumni@jaguar.dote.hu
Antal-Szalmás
Péter
P
Aleksza
Magdolna
M
Kovács
Ildikó
I
Sipka
Sándor
S
Zeher
Margit
M
Kiss
Emese
E
Szegedi
Gyula
G
D000328:Adult; D015536:Down-Regulation; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D008180:Lupus Erythematosus, Systemic / Q000188:drug therapy* / Q000276:immunology; D008297:Male; D016260:Monocytes, Activated Killer / Q000187:drug effects / Q000276:immunology*
D005938:Glucocorticoids; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides