Human sulfotransferases and their role in chemical metabolism.
Sulfonation is an important reaction in the metabolism of numerous xenobiotics, drugs, and endogenous compounds. A supergene family of enzymes called sulfotransferases (SULTs) catalyze this reaction. In most cases, the addition of a sulfonate moiety to a compound increases its water solubility and decreases its biological activity. However, many of these enzymes are also capable of bioactivating procarcinogens to reactive electrophiles. In humans three SULT families, SULT1, SULT2, and SULT4, have been identified that contain at least thirteen distinct members. SULTs have a wide tissue distribution and act as a major detoxification enzyme system in adult and the developing human fetus. Nine crystal structures of human cytosolic SULTs have now been determined, and together with site-directed mutagenesis experiments and molecular modeling, we are now beginning to understand the factors that govern distinct but overlapping substrate specificities. These studies have also provided insight into the enzyme kinetics and inhibition characteristics of these enzymes. The regulation of human SULTs remains as one of the least explored areas of research in the field, though there have been some recent advances on the molecular transcription mechanism controlling the individual SULT promoters. Interindividual variation in sulfonation capacity may be important in determining an individual's response to xenobiotics, and recent studies have begun to suggest roles for SULT polymorphism in disease susceptibility. This review aims to provide a summary of our present understanding of the function of human cytosolic sulfotransferases.
Gamage
Niranjali
N
School of Biomedical Sciences, School of Molecular and Microbial Sciences, and Institute for Molecular Bioscience, University of Queensland, Queensland 4072, Australia.
Barnett
Amanda
A
Hempel
Nadine
N
Duggleby
Ronald G
RG
Windmill
Kelly F
KF
Martin
Jennifer L
JL
McManus
Michael E
ME
D000818:Animals; D001711:Biotransformation; D003460:Crystallization; D006801:Humans; D007527:Isoenzymes; D008958:Models, Molecular; D011487:Protein Conformation; D015238:Sulfotransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015262:Xenobiotics / Q000493:pharmacokinetics*
D007527:Isoenzymes; D015262:Xenobiotics; D015238:Sulfotransferases