[A study on stem cell mobilization after partial liver transplantation in rats].
OBJECTIVE
To explore the role of stem cell mobilization on regeneration of partially grafted livers.


METHODS
Rats models with cross-sex 50% PLTx (partial liver transplantation) were established. The rats were divided into three groups: PLTx, WLTx (whole liver transplantation) and sham operation groups. Bone marrow and liver samples were collected on days 1, 3, 5, 7 postoperatively (each n = 6). The quantitative variations of the cells with stem cell markers in the bone marrow, including beta2m-/Thy-1.1+, CD45+/CD34+, Flt2/3+ and c-kit+ markers, were detected using flow cytometry. Sry gene positive cells in donor livers were detected by fluorescent in situ hybridization (FISH), and the expressions of CD34, c-kit and Thy-1.1 were detected by immunohistochemistry technique.


RESULTS
Compared with the WLTx and sham operation groups, beta2m-/Thy-1.1+, CD45+/CD34+ cells in bone marrows in the PLTx group increased on the first postoperative day and decreased on the following days. The CD34, c-kit and Thy-1.1 positive cells detected in portal tract areas peaked during the 3-5 postoperative days. CD34+/CD45+ positive cells could be detected. The expressions of CD34, c-kit and Thy-1.1 positive cells were rare in the WLTx and sham operation groups. Sry+ cells could be detected in portal tract areas and few Sry+/CD34+ and Sry+/Thy-1.1+cells were detected.


CONCLUSIONS
In the PLTx group, the stem cells in the bone marrow were mobilized and stem cells in the liver were activated.
Liu
Feng
F
Institute of Hepatology, People's Hospital, Peking University, Beijing 100044, China.
Wei
Lai
L
Chen
Guo-dong
GD
Pan
Xiao-ben
XB
Cong
Xu
X
Fei
Ran
R
D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D017493:Leukocyte Common Antigens / Q000276:immunology; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology; D018800:Thy-1 Antigens / Q000276:immunology
D018952:Antigens, CD34; D018800:Thy-1 Antigens; D017493:Leukocyte Common Antigens