title: Histone deacetylase 1 is essential for oligodendrocyte specification in the zebrafish CNS.
abstract: Histone deacetylases are critical components of transcriptional silencing mechanisms that regulate embryonic development. Recent work has shown that histone deacetylase 1 (hdac1) is required for neuronal specification during zebrafish CNS development. We show here that specification of oligodendrocytes, the myelinating cells of the CNS, also fails to occur in the hdac1 mutant hindbrain, but persistence of neural progenitors in the hindbrain ventricular zone, which express pax6a and sox2, is independent of hdac1 activity. Commitment of ventral neural progenitors to the oligodendrocyte fate is thought to require co-ordinate, hedgehog-dependent expression of olig2 and nkx2.2a in these cells, leading to expression of sox10 and subsequent differentiation of oligodendrocytes. Remarkably, transcription of olig2 is extinguished in ventral neural progenitors of the hdac1 mutant hindbrain, whereas expression of nkx2.2a is up-regulated in these cells, and sox10 expression is suppressed. Our results identify hdac1 as a novel, essential component of the mechanism that allocates neural progenitors to the oligodendrocyte fate, by attenuating expression of a subset of neural progenitor genes and rendering olig2 expression responsive to Hedgehog signalling.
authors.affiliation: Department of Biomedical Science, Centre for Developmental and Biomedical Genetics, University of Sheffield, Firth Court, Western Bank, Sheffield, South Yorks, S10 2TN, UK. v.t.cunliffe@shef.ac.uk
authors.forename: Vincent T
authors.initials: VT
authors.lastname: Cunliffe
authors.forename: Patrizia
authors.initials: P
authors.lastname: Casaccia-Bonnefil
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D002454:Cell Differentiation; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology* / Q000201:enzymology*; D018507:Gene Expression Regulation, Developmental; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins / Q000235:genetics; D017403:In Situ Hybridization; D009419:Nerve Tissue Proteins / Q000235:genetics; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000166:cytology / Q000201:enzymology*; D012249:Rhombencephalon / Q000166:cytology / Q000196:embryology / Q000201:enzymology; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology; D014157:Transcription Factors / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D056284:Histone Deacetylase 1; D006655:Histone Deacetylases; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D014157:Transcription Factors; D029961:Zebrafish Proteins; C465361:olig2 protein, zebrafish; C488363:HDAC1 protein, zebrafish