title: [Detection of glucocorticoid receptor-alpha mRNA expression using FQ-RT-PCR in nasal polyp].
abstract: OBJECTIVE
  To detect the expression of the glucocorticoid receptor-alpha (GR-alpha) mRNA in nasal polyp and normal nasal mucosa and investigate the significance of GR-alpha in progression of nasal polyp.
  
  
  METHODS
  The expression of GR-alpha mRNA in 36 samples of nasal polyp and 31 samples of normal nasal mucosa was examined by using fluorescent quantitative PCR(FQ RT-PCR).
  
  
  RESULTS
  The GR-alpha mRNA levels in normal nasal mucosa [(135.4 +/- 5.2) x 10(10) copy/g] were significantly higher than those in nasal polyp [(25.5 +/- 5.0) x 10(10) copy/g] (P < 0.01).
  
  
  CONCLUSIONS
  The low expression of GR-alpha mRNA in nasal polyp is related to the progression of nasal polyp.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, China. lp76@tom.com
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Zhang
authors.forename: Gehua
authors.initials: G
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Ye
authors.forename: Yanan
authors.initials: Y
authors.lastname: Sun
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
mesh_terms: D000328:Adult; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D009298:Nasal Polyps / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; C411470:glucocorticoid receptor alpha