Molecular cloning, expression and chromosomal localization of mouse MM-1.
The protooncogene product Myc associates with many proteins. The isolation of the mouse MM-1; c-Myc binding protein (Myc-Modulator 1) cDNA is described. The cDNA contains a 462 bp open reading frame that encodes a polypeptide of 154 amino acid residues. The deduced amino acid sequence indicates that mouse MM-1 has a 99% identity with the sequence of human MM-1. The expression of mouse MM-1 mRNA was detected in the fetal liver, but its level was 3-fold higher than that in the normal adult liver, and was slightly increased after a partial hepatectomy. It is expressed widely in a variety of adult mouse tissues. Thus, MM-1 may play a role in liver development and growth. A bioinformatics analysis indicates that mouse MM-1 gene consists of 6 exons. Furthermore, the chromosomal location of the mouse MM-1 gene was on the F2-F3 band of chromosome 15, as determined by fluorescence in situ hybridization.
Inazu
Tetsuya
T
Department of Biochemistry, Fukui Medical University, Matsuoka, Fukui 910-1193, Japan. tinazu@saigata-nh.go.jp
Myint
Zaw
Z
Kuroiwa
Asato
A
Matsuda
Yoichi
Y
Noguchi
Tamio
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D016259:Genes, myc / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008099:Liver / Q000378:metabolism; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D002352:Carrier Proteins; D018076:DNA, Complementary; C513593:MM-1 protein, mouse