title: Protective effect of melatonin on human peripheral blood hematopoeitic stem cells against doxorubicin cytotoxicity.
abstract: BACKGROUND
  The dose-limiting toxicity of doxorubicin on hematopoietic stem cells reduces the maximum benefit from this powerful drug. Melatonin may play a role in reducing this toxicity.
  
  
  METHODS
  Melatonin at 10 microM was used while challenging human peripheral blood stem cells (PBSC) with doxorubicin (0.6 microM and 1 microM), and colony formation was used to evaluate the protective effect of melatonin.
  
  
  RESULTS
  Melatonin was protective for the myeloid and erythroid series when given during or 1 hour after, but not before, doxorubicin, as measured by colony assay. This protection was independent from its antioxidant function as measured by 2', 7'-dichlodihydro-fluorescein diacetate and was selective for PBSC when compared to the MCF-7 cancer cell line.
  
  
  CONCLUSIONS
  The results suggest the importance of the time sequence for melatonin administration to exert its protective effect in relation to doxorubicin treatment, as well as its protective effect on both erythroid and myeloid elements independent from its antioxidant function.
authors.affiliation: Department of Cardiovascular Surgery, Kumamoto University Graduate School of Medical Sciences, Japan.
authors.forename: Khaled
authors.initials: K
authors.lastname: Greish
authors.forename: Isao
authors.initials: I
authors.lastname: Sanada
authors.forename: Alla El-Dein
authors.initials: Ael-D
authors.lastname: Saad
authors.forename: Ehab
authors.initials: E
authors.lastname: Hasanin
authors.forename: Michio
authors.initials: M
authors.lastname: Kawasuji
authors.forename: Fumio
authors.initials: F
authors.lastname: Kawano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maeda
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology; D001943:Breast Neoplasms / Q000188:drug therapy; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology / Q000633:toxicity*; D004347:Drug Interactions; D006402:Hematologic Diseases / Q000139:chemically induced / Q000517:prevention & control; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008550:Melatonin / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D017382:Reactive Oxygen Species; D004317:Doxorubicin; D006861:Hydrogen Peroxide; D008550:Melatonin