Copro-DNA tests for diagnosis of animal taeniid cestodes.
PCR has proven its value for the diagnosis of taeniid cestodes in animal definitive hosts, although only few specific tests are available at the moment (Echinococcus multilocularis, Echinococcus granulosus 'sheep strain'). Additional tests with specificities for further taeniids are urgently needed and new tests are currently being developed, e.g. a multiplex PCR for simultaneous detection of E. multilocularis, E. granulosus (all strains) and Taenia spp. (all species). PCR is a technically demanding and expensive technique: DNA isolation from faecal specimens remains a laborious task because of the presence of PCR-inhibitory substances, and special precautions need to be taken to avoid false-positive results due to cross-contamination of amplification reactions. PCR, therefore, is mainly used for confirmative purposes of coproantigen-positive samples or for identification of taeniid eggs recovered from faecal specimens or from environmental samples.
Mathis
Alexander
A
Institute of Parasitology, Vetsuisse-Faculty, University of Zurich, Switzerland. alexander.mathis@access.unizh.ch
Deplazes
Peter
P
D000818:Animals; D018509:DNA, Helminth / Q000032:analysis* / Q000302:isolation & purification; D004443:Echinococcosis / Q000175:diagnosis* / Q000469:parasitology; D004446:Echinococcus / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods; D013621:Taenia / Q000235:genetics / Q000302:isolation & purification; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
D018509:DNA, Helminth