L-Arginine-NO-cGMP signaling following acute liver injury in the rat.
The incidence of liver diseases has increased over the past few years. For this reason, the consequences of induced nitric oxide (NO) synthesis in liver damages warrant further studies. To address this issue, we investigated the expression of key enzymes engaged in the control of NO signaling in the rat liver after carbon tetrachloride (CCl4) intoxication and subsequent regeneration. CCl4 intoxication resulted in up-regulation of the entire NO signal transduction machinery. Expression patterns of arginase, soluble guanylyl cyclase and cyclic nucleotide phosphodiesterase revealed striking parallels with that of NO synthase (NOS). Co-expression of the major components of the l-arginine-NO-cGMP signaling cascade both in hepatocytes and in nonparenchymal cells indicates an autocrine rather than a paracrine fashion of NO signaling in the liver. Up-regulation of NOS after CCl4 intoxication fell behind the oxidative stress and was found to be associated with the initiation of parenchymal regeneration implying a beneficial effect of NO.
Minin
Evgeny A
EA
Gerhard Domagk Institute of Pathology, University of Muenster, Domagkstr. 17, 48149 Muenster, Germany.
Buchwalow
Igor B
IB
Wellner
Maren
M
Palmes
Daniel
D
Spiegel
Hans-Ullrich
HU
Neumann
Joachim
J
Boecker
Werner
W
Herbst
Hermann
H
D000818:Animals; D001120:Arginine / Q000502:physiology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D006152:Cyclic GMP / Q000502:physiology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008115:Liver Regeneration; D008297:Male; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*
D012333:RNA, Messenger; D009569:Nitric Oxide; D001120:Arginine; D002251:Carbon Tetrachloride; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP