Co-overexpression of fibroblast growth factor 3 and epidermal growth factor receptor is correlated with the development of nonsmall cell lung carcinoma.
BACKGROUND
Lung cancer is a prevalent cancer with a poor prognosis. To develop a useful in vitro cell model, a cell line of lung squamous cell carcinoma (SCC-35) was established.


METHODS
The SCC-35 cell was characterized by comparative genomic hybridization (CGH) and spectral karyotyping (SKY). Chromosome microdissection, fluorescence in situ hybridization (FISH), and Southern and Northern blots analyses were used to study target genes.


RESULTS
Two amplicons were found at chromosomes 7p12 and 11q13. Amplification and overexpression of epidermal growth factor receptor (EGFR) at 7p12 and fibroblast growth factor 3 (FGF3) at 11q13 were found. To understand the correlation between these two genes in nonsmall cell lung carcinoma (NSCLC) more comprehensively, overexpression of FGF3 and EGFR was investigated by immunohistochemistry with a tissue microarray containing 406 NSCLC samples. Cytoplasmic overexpression of FGF3 and EGFR was detected in 61% and 69% NSCLC cases, respectively. More interestingly, a significant correlation between overexpression of FGF3 and EGFR was found in NSCLC.


CONCLUSIONS
These results suggest that co-overexpression of FGF3 and EGFR may play an important role in the pathogenesis of lung carcinoma.
Tai
Amy L S
AL
Department of Clinical Oncology, the University of Hong Kong, Hong Kong, China.
Sham
Jonathan S T
JS
Xie
Dan
D
Fang
Yan
Y
Wu
Yi-Long
YL
Hu
Liang
L
Deng
Wen
W
Tsao
George S W
GS
Qiao
Gui-Bin
GB
Cheung
Annie L M
AL
Guan
Xin-Yuan
XY
D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D051518:Fibroblast Growth Factor 3 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D032681:Spectral Karyotyping; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D046888:Tissue Array Analysis
C496444:FGF3 protein, human; D051518:Fibroblast Growth Factor 3; D066246:ErbB Receptors; D019098:Telomerase