In vitro effects of some anaesthetic drugs on lactoperoxidase enzyme activity.
In vitro effects of ketamine and bupivacaine drugs on bovine lactoperoxidase (LPO; E.C. 1.11.1.7) enzyme activity were investigated. Lactoperoxidase was purified with Amberlite CG 50 resin, CM Sephadex C-50 ion-exchange chromatography, and Sephadex G-100 gel filtration chromatography from skimmed bovine milk. Rz(A412/A280) value for the purified LPO was found to be 0.8. Inhibition or activation effects of the drugs on LPO enzyme were determined using 2,2(1)-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (ABTS) as a chromogenic substrate at pH = 6.0. The I50 values of ketamine and bupivacaine were 0.29 mM and 0.155 mM, respectively and the K(i) constants for ketamine and bupivacaine were 0.019 +/- 0.031 and 0.015 +/- 0.021 mM, respectively; they were non-competitive inhibitors.
Ozdemir
Hasan
H
Faculty of Arts and Sciences, Department of Chemistry, Atatürk University, 25240 Erzurum, Turkey. hozdemir@atauni.edu.tr
Uğuz
Metin Tansu
MT
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D002045:Bupivacaine / Q000494:pharmacology*; D002417:Cattle; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007649:Ketamine / Q000494:pharmacology*; D007784:Lactoperoxidase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism*
D000777:Anesthetics; D004791:Enzyme Inhibitors; D007649:Ketamine; D007784:Lactoperoxidase; D002045:Bupivacaine