Sexual reproduction in subcultures of Absidia blakesleeana after years of preservation under mineral oil.
Two subcultures of Absidia blakesleeana IOC No. 2425 (-) mating type strain preserved in 1959 and 1981 on a thick layer of potato dextrose agar (PDA) medium under sterile mineral oil and maintained at room temperature in the Fungal Cultural Collection of Institute Oswaldo Cruz--IOC--were paired with the defined Absidia blakesleeana URM-UFP No. 2076 (+) mating type strain. Portions of the line where mycelia of the two strains met were observed under light microscopy. Zygospores belonging to Subgenus Mycocladus were observed. The results demonstrated conservation and re-establishment of the physiological and genetic metabolic processes of two subcultures preserved under mineral oil.
Santos
Manoel J S
MJ
Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz-IOC-FIOCRUZ, Rio de Janeiro, Brazil. mjsantos@ioc.fiocruz.br
Trufem
Sandra F B
SF
de Oliveira
Pedrina C
PC
D020095:Absidia / Q000254:growth & development / Q000502:physiology*; D000362:Agar; D005947:Glucose; D008899:Mineral Oil; D009172:Mycology / Q000379:methods*; D011309:Preservation, Biological / Q000379:methods*; D012098:Reproduction; D011198:Solanum tuberosum; D013696:Temperature; D013997:Time Factors
D008899:Mineral Oil; D000362:Agar; D005947:Glucose