title: Mangifera indica L. (Vimang) protection against serum oxidative stress in elderly humans.
abstract: BACKGROUND
  We searched for the protective effect of a natural extract from stem bark of Mangifera indica L. extract (Vimang) on age-related oxidative stress.
  
  
  METHODS
  Healthy subjects were classified in two groups, elderly (>65 years) and young group (<26 years). The elderly group received a daily dose of 900 mg of extract (three coated Vimang tablets, 300 mg each, before meals) for 60 days. Serum concentration of lipid peroxides, serum peroxidation potential, extracellular superoxide dismutase activity (EC-SOD), glutathione status (GSH, GSSG, GSSG/GSH ratio)) and total antioxidant status (TAS) were determined before (both experimental groups) and 15, 30, and 60 days after treatment (only elderly group). We confirmed the existence of an age-associated oxidative stress in human serum as documented by an age-related increase in serum lipoperoxides and GSSG and a decrease in serum antioxidant capacity and EC-SOD activity.
  
  
  RESULTS
  Vimang tablet supplementation increased EC-SOD activity (p <0.01) and serum TAS (p <0.01). It also decreased serum thiobarbituric reactive substances (p <0.01) and GSSG levels (p <0.05). We suggested that the antioxidant components of the extract could have been utilized by the cells (especially blood and endothelial cells), sparing the intra- and extracellular antioxidant system and increasing serum peroxil scavenging capacity, thus preventing age-associated increase in GSH oxidation and lipoperoxidation.
  
  
  CONCLUSIONS
  Vimang tablets prevent age-associated oxidative stress in elderly humans, which could retard the onset of age-associated disease, improving the quality of life for elderly persons.
authors.affiliation: Laboratorio de Farmacología, Departamento de Investigaciones Biomédicas, Centro de Química Farmacéutica, Atabey, Playa, Ciudad de La Habana, Cuba. g031071@yahoo.com
authors.forename: Gilberto L
authors.initials: GL
authors.lastname: Pardo-Andreu
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Philip
authors.forename: Annia
authors.initials: A
authors.lastname: Riaño
authors.forename: Carlos
authors.initials: C
authors.lastname: Sánchez
authors.forename: Carmen
authors.initials: C
authors.lastname: Viada
authors.forename: Alberto J
authors.initials: AJ
authors.lastname: Núñez-Sellés
authors.forename: René
authors.initials: R
authors.lastname: Delgado
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D000975:Antioxidants / Q000032:analysis; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D031022:Mangifera; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage*; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; C501277:Mangifera indica extract; D010936:Plant Extracts; D013482:Superoxide Dismutase; D005978:Glutathione