Dual in vivo magnetic resonance evaluation of magnetically labeled mouse embryonic stem cells and cardiac function at 1.5 t.
Cell therapy has demonstrated the potential to restore injured myocardium. A reliable in vivo imaging method to localize transplanted cells and monitor their restorative effects will enable a systematic investigation of this therapeutic modality. The dual MRI capability of imaging both magnetically labeled mouse embryonic stem cells (mESC) and their restorative effects on cardiac function in a murine model of acute myocardial infarction is demonstrated. Serial in vivo MR detection of transplanted mESC and monitoring of the mESC-treated myocardium was conducted over a 4-week period using a 1.5 T clinical scanner. During the 4-week duration, the mESC-treated myocardium demonstrated sustained improvement of the left ventricular (LV) ejection fraction and conservation of LV mass. Furthermore, no significant difference of their restorative effects on the cardiac function was created by the magnetic labeling of mESC. Thus, in vivo MRI enables simultaneous detection of transplanted mESC and their therapeutic effect on the injured myocardium.
Arai
Takayasu
T
Division of Cardiovascular Medicine, Stanford University, California 94305-5233, USA.
Kofidis
Theo
T
Bulte
Jeff W M
JW
de Bruin
Jorg
J
Venook
Ross D
RD
Berry
Gerald J
GJ
Mcconnell
Michael V
MV
Quertermous
Thomas
T
Robbins
Robert C
RC
Yang
Phillip C
PC
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003287:Contrast Media; D003911:Dextrans; D004195:Disease Models, Animal; D005260:Female; D052203:Ferrosoferric Oxide; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents; D007501:Iron; D008164:Luminescent Proteins; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D009203:Myocardial Infarction / Q000628:therapy*; D010087:Oxides; D013194:Staining and Labeling; D033581:Stem Cell Transplantation
D003287:Contrast Media; D003911:Dextrans; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D058185:Magnetite Nanoparticles; D010087:Oxides; D049452:Green Fluorescent Proteins; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide