In vitro responses of rat alveolar macrophages to particle suspensions and water-soluble components of dust storm PM(2.5).
A study was conducted to investigate the in vitro toxicities of dust storm particulate matter with an aerodynamic diameter < or = 2.5 microm (PM(2.5)) on rat alveolar macrophages (AM). This was based on the ambient PM(2.5) collected in March 2004 from Baotou city, Inner Mongolia Autonomous Region, China. The particles were classified as normal (from sunshiny and clean days) and dust storm samples according to the dust storm classification, and the local weather and air quality monitoring data. The cell viability, levels of cellular thiobarbituric acid-reactive species (TBARS), glutathione (GSH), and cytosolic free calcium ions (Ca(2+)), and the plasma membrane ATPase activities and membrane lipid fluidity were determined 4h following the in vitro treatment of AM with differing dosages of the collected samples. Results revealed that dust storm PM(2.5) and their water-soluble fractions at high dosages generated oxidant stress on AM, induced leakage of lactate dehydrogenase (LDH), significantly decreased activities of plasma membrane Na(+)K(+)-ATPase, increased intracellular Ca(2+) levels, and led to significant alterations in membrane lipid fluidity, finally resulting in cytotoxicity. A two-way ANOVA showed that there was no significant difference in the above indices measured between the normal and dust storm PM(2.5), suggesting the deleterious effects of ambient PM(2.5) from Baotou city were based on the dose used rather than the type of particles. But due to a higher concentration of airborne PM(2.5) mass concentration in dust storm days than that in normal days, it concluded that during dust storm episodes, a much more serious effect on AM was imminent.
Geng
Hong
H
Institute of Environmental Medicine and Toxicology, Shanxi University, Taiyuan 030006, PR China.
Meng
Ziqiang
Z
Zhang
Quanxi
Q
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D004391:Dust / Q000032:analysis; D005978:Glutathione / Q000032:analysis; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D016676:Macrophages, Alveolar / Q000187:drug effects*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013535:Suspensions
D000393:Air Pollutants; D004391:Dust; D013535:Suspensions; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione; D002118:Calcium