title: Ginsenoside extraction from Panax quinquefolium L. (American ginseng) root by using ultrahigh pressure.
abstract: A new method of ultrahigh pressure extraction (UPE) was used to extract the ginsenosides from Panax quinquefolium L. (American ginseng) root at room temperature. Several solvents, including water, ethanol, methanol, and n-butanol were used in the UPE. The ginsenosides were quantified by a HPLC equipped with UV-vis detector. The results showed that ethanol is the most efficient solvent among the used ones. Compared with other methods, i.e., Soxhlet extraction, heat reflux extraction, ultrasound-assisted extraction, microwave-assisted extraction, and supercritical CO2 extraction, the UPE has the highest extraction yield in the shortest time. The extraction yield of 0.861% ginsenoside-Rc in 2 min was achieved by the UPE, while the yields of 0.284% and 0.661% were obtained in several hours by supercritical CO2 extraction and the heat reflux extraction, respectively.
authors.affiliation: College of Biological and Agricultural Engineering, Jilin University, Changchun, China.
authors.forename: Shouqin
authors.initials: S
authors.lastname: Zhang
authors.forename: Ruizhan
authors.initials: R
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
authors.forename: Changzheng
authors.initials: C
authors.lastname: Wang
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000431:Ethanol / Q000494:pharmacology; D036145:Ginsenosides / Q000032:analysis* / Q000302:isolation & purification*; D006358:Hot Temperature; D008956:Models, Chemical; D005894:Panax / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D011312:Pressure; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D036145:Ginsenosides; D012997:Solvents; D002245:Carbon Dioxide; D000431:Ethanol