title: Location and base selectivity on fragmentation of brominated oligodeoxynucleotides.
abstract: Bromine-modified oligodeoxynucleotides (ODNs) were fragmented in the electrospray source to study the influence of brominated bases on fragmentation. Several 13-mer ODNs containing a brominated pyrimidine base, BrdU (5-bromodeoxyuridine) or BrdC (5-bromodeoxycytidine), were analyzed. Low cone voltage fragmentation yields a loss of the brominated base with a preferential loss for the brominated base closer to the 5'-end (2-position > 4-position > 12-position) as well as a preferential loss of BrdU over BrdC. Higher cone voltage produces backbone fragmentation with complementary a(n)-base and w(m) ions close to the brominated base. On the basis of these observations, we located the brominated base in the sequence for all of the ODNs studied.
authors.affiliation: Centre de Biophysique Moléculaire, CNRS UPR 4301, University of Orléans and INSERM, rue Charles Sadron, 45071 Orléans Cedex 02, France. bure@cnrs-orleans.fr
authors.forename: Corinne
authors.initials: C
authors.lastname: Buré
authors.forename: Bertrand
authors.initials: B
authors.lastname: Castaing
authors.forename: Catherine
authors.initials: C
authors.lastname: Lange
authors.forename: Agnès F
authors.initials: AF
authors.lastname: Delmas
mesh_terms: D000465:Algorithms; D001966:Bromine / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000032:analysis* / Q000528:radiation effects; D011743:Pyrimidines / Q000032:analysis; D014466:Ultraviolet Rays
chemical_list: D007202:Indicators and Reagents; D009841:Oligonucleotides; D011743:Pyrimidines; D001966:Bromine