[High resolution DNA typing for HLA-A, B, Cw genes by polymerase chain reaction-sequence specific oligonucleotide probes method in the Shenyang Han population].
OBJECTIVE
To investigate, at the DNA level, the polymorphism of HLA-A, -B, -Cw genes in the Chinese of Han ethnicity in Shenyang.


METHODS
Hybridization with polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) was used to determine HLA-A, -B and -Cw genotypes of 108 unrelated healthy individuals from a Chinese Han population. These Hans were born and living in the Shenyang area.


RESULTS
The numbers of alleles identified were 21 for HLA-A, 43 for HLA-B, and 23 for HLA-Cw. All the allele frequency distributions were consistent with the Hardy-Weinberg equilibrium.


CONCLUSIONS
Using molecular method, the present authors have analyzed the characteristic of HLA I distribution in a group of indigenous Hans in Shenyang and thus have provided more accurate gene data for use in related researches.
Han
Shi-xin
SX
Department of Dermatology, First Hospital of Dalian Medical University, Dalian, Liaoning, P. R. China.
Shang
Ying-bin
YB
Li
Jiu-hong
JH
Geng
Long
L
Zhai
Ning
N
Cao
Kai
K
Song
Fang-ji
FJ
D000293:Adolescent; D000328:Adult; D000483:Alleles; D002681:China; D004247:DNA / Q000032:analysis / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D055815:Young Adult
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D015345:Oligonucleotide Probes; D004247:DNA