Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.
Functional analysis of the proteome is an essential part of genomic research. To facilitate different proteomic approaches, a MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a sequence-verified ORF as a C-terminal ORF fusion protein, under regulated control. Analysis of 5573 MORFs demonstrates that nearly all verified ORFs are expressed, suggests the authenticity of 48 ORFs characterized as dubious, and implicates specific processes including cytoskeletal organization and transcriptional control in growth inhibition caused by overexpression. Global analysis of glycosylated proteins identifies 109 new confirmed N-linked and 345 candidate glycoproteins, nearly doubling the known yeast glycome.
Gelperin
Daniel M
DM
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.
White
Michael A
MA
Wilkinson
Martha L
ML
Kon
Yoshiko
Y
Kung
Li A
LA
Wise
Kevin J
KJ
Lopez-Hoyo
Nelson
N
Jiang
Lixia
L
Piccirillo
Stacy
S
Yu
Haiyuan
H
Gerstein
Mark
M
Dumont
Mark E
ME
Phizicky
Eric M
EM
Snyder
Michael
M
Grayhack
Elizabeth J
EJ
D048708:Cell Growth Processes / Q000235:genetics; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics; D015723:Gene Library; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics*; D016366:Open Reading Frames; D020543:Proteome; D040901:Proteomics / Q000379:methods; D015003:Yeasts / Q000235:genetics*
D005656:Fungal Proteins; D006023:Glycoproteins; D020543:Proteome