[Effects of tea polyphenols on cell proliferation and hTERT of human Tca8113 cell lines].
OBJECTIVE
To investigate the effects of tea polyphenols (TP) on cell proliferation and human telomerase reverse transcriptase (hTERT).


METHODS
The experiment was divided into tea polyphenols 0.100 g/L, tea polyphenols 0.050 g/L and blank control groups. The inhibitory ratio of cell proliferation was assayed with MTT, and hTERT mRNA was detected by RT-PCR, and hTERT protein analyzed by Western-blotting. The data were analyzed by one-way ANOVA and Student-Newman-Keuls of SPSS 11.0 for windows.


RESULTS
Cell proliferations were significantly inhibited after exposure to TP. The proliferation inhibiting rate of TP 0.025, 0.050, 0.100, 0.200 g/L on Tca8113 cell at 72 h was 69.75% +/- 3.24%, 63.17% +/- 3.19%, 50.35% +/- 4.21% and 34.75% +/- 3.71%, respectively (F = 270.19, P < 0.05). At the end of 72 h, the hTERT mRNA expression in TP 0.100 g/L, 0.050 g/L and control group were 0.32 +/- 0.05, 0.41 +/- 0.04, 0.72 +/- 0.05, respectively (P < 0.05). The Western-blotting assay showed that hTERT protein was also decreased by tea polyphenols compared to control group.


CONCLUSIONS
Tea polyphenols could inhibit the proliferation of Tca8113 cells and expression of the hTERT mRNA and protein in Tca8113 cell lines. This effect might be one of the mechanisms for anticancer function of tea polyphenols.
Yao
Hua
H
Department of Stomatology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
Wang
Hui-ming
HM
Wu
Qiu-liang
QL
Fan
Jun
J
D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D012333:RNA, Messenger / Q000378:metabolism; D013662:Tea / Q000737:chemistry*; D019098:Telomerase / Q000378:metabolism*; D014062:Tongue Neoplasms / Q000201:enzymology / Q000473:pathology*
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D012333:RNA, Messenger; D013662:Tea; C509186:TERT protein, human; D019098:Telomerase