[The RT-PCR-identified Fas and FasL expression and apoptosis in mouse hepatoma MH-22a and histiocytic sarcoma J-774 clonal lines coupled with in vitro cultivation with syngenic splenocytes].
Malignant growth is associated with various patterns of interaction between tumor cells and those of the body immune system, interaction between Fas-receptor (Fas) and Fas-ligand (FasL) expression being one of them. These mechanisms were simulated in vitro using the main cell populations from murine hepatoma MH-22a, histiocytic sarcoma J-774 and their clonal lines obtained from cocultivation of tumor cells and syngenic splenocytes. Fas and FasL expression was identified by the RT-PCR method while apoptosis--by electrophoresis of low molecular DNA fractions and clonogenic survival.
Aleksandrova
S A
SA
Ginkul
L B
LB
Shvemberger
I N
IN
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D002999:Clone Cells; D018920:Coculture Techniques; D004273:DNA, Neoplasm / Q000032:analysis; D004586:Electrophoresis; D053222:Fas Ligand Protein; D015972:Gene Expression Regulation, Neoplastic; D006644:Histiocytes; D008114:Liver Neoplasms, Experimental / Q000737:chemistry*; D008562:Membrane Glycoproteins / Q000032:analysis*; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012513:Sarcoma, Experimental / Q000737:chemistry*; D013154:Spleen / Q000166:cytology*; D048069:Tumor Necrosis Factors / Q000032:analysis*; D019014:fas Receptor / Q000032:analysis*
D004273:DNA, Neoplasm; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D048069:Tumor Necrosis Factors; D019014:fas Receptor