Evaluation of automated sex hormone binding globulin immunoassays.
BACKGROUND
Sex hormone binding globulin (SHBG) is an important regulator of testosterone and estradiol.


METHODS
We validated the Diagnostic Products Corporation (DPC) and Roche Diagnostic SHBG immunoassays on the DPC Immulite 2000 and Roche Modular E170 analyzers.


RESULTS
The coefficient of variation for SHBG kits from both manufacturers was in the range of 3.9-7.7% (between-run) and 0.95-5.0% (within-run), free of interference from hemoglobin, bilirubin, lipid, and rheumatoid factor, and linear up to at least 170 nM SHBG. The results of the two methods, however, were biased by up to 29% depending on the SHBG concentration.


CONCLUSIONS
The SHBG assays perform well but standardization is needed.
Jin
Ming
M
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA.
Wener
Mark H
MH
Bankson
Daniel D
DD
D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D016014:Linear Models; D015203:Reproducibility of Results; D012738:Sex Hormone-Binding Globulin / Q000032:analysis*; D013739:Testosterone
D012738:Sex Hormone-Binding Globulin; D013739:Testosterone