title: P-glycoprotein expression in lamina propria lymphocytes of duodenal biopsy samples in dogs with chronic idiopathic enteropathies.
abstract: P-glycoprotein (p-gp) is a transmembrane protein functioning as a drug-efflux pump in the intestinal epithelium. Human patients with inflammatory bowel disease (IBD) who fail to respond to treatment with steroids express high levels of p-gp in lamina propria lymphocytes. The purpose of this study was to investigate p-gp expression in duodenal biopsy samples of dogs with chronic enteropathies and to evaluate the expression of p-gp after treatment with a known inducer of p-gp (prednisolone). Duodenal biopsy samples from 48 dogs were evaluated immunohistochemically with the mouse monoclonal antibody C219 for expression of p-gp in lamina propria lymphocytes. Biopsy samples were available from 15 dogs after treatment with prednisolone and 16 dogs after dietary therapy alone ("elimination diet"). Treatment with prednisolone resulted in an increase in p-gp expression (P=0.005). In contrast, dietary treatment alone produced no significant change in p-gp expression (P=0.59). A low p-gp score before initiation of steroid treatment was significantly associated with a positive response to treatment (P=0.01). These results indicate that lamina propria lymphocyte expression of p-gp is upregulated after prednisolone treatment in dogs with IBD, and that mucosal expression of p-gp may be of value in predicting the response to therapy.
authors.affiliation: Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
authors.forename: K
authors.initials: K
authors.lastname: Allenspach
authors.forename: P J
authors.initials: PJ
authors.lastname: Bergman
authors.forename: S
authors.initials: S
authors.lastname: Sauter
authors.forename: A
authors.initials: A
authors.lastname: Gröne
authors.forename: M G
authors.initials: MG
authors.lastname: Doherr
authors.forename: F
authors.initials: F
authors.lastname: Gaschen
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000627:therapeutic use*; D000818:Animals; D001706:Biopsy; D004032:Diet; D004283:Dog Diseases / Q000276:immunology / Q000378:metabolism*; D004285:Dogs; D004386:Duodenum / Q000378:metabolism*; D005260:Female; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy / Q000378:metabolism / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D011239:Prednisolone / Q000627:therapeutic use; D015854:Up-Regulation
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000305:Adrenal Cortex Hormones; D011239:Prednisolone