Molecular forensic profiling of Cryptosporidium species and genotypes in raw water.
The emerging concept of host specificity of Cryptosporidium spp. was exploited to characterize sources of fecal contamination in a watershed. A method of molecular forensic profiling of Cryptosporidium oocysts on microscope slides prepared from raw water samples processed by U.S. Environmental Protection Agency Method 1623 was developed. The method was based on a repetitive nested PCR-restriction fragment length polymorphism-DNA sequencing approach that permitted the resolution of multiple species/genotypes of Cryptosporidium in a single water sample.
Ruecker
Norma J
NJ
Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
Bounsombath
Niravanh
N
Wallis
Peter
P
Ong
Corinne S L
CS
Isaac-Renton
Judith L
JL
Neumann
Norman F
NF
D000818:Animals; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D005554:Forensic Medicine; D020869:Gene Expression Profiling / Q000379:methods*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014481:United States; D014484:United States Environmental Protection Agency; D014871:Water Microbiology; D014876:Water Pollution