Unbiased examination of changes in plasma membrane proteins in drug resistant cancer cells.
In this study, an unbiased examination is made of the abundance changes between proteins found in the basolateral plasma membranes of a drug susceptible parental MCF-7 breast cancer cell line and a cell line selected from the parent line for resistance to the anticancer drug mitoxantrone. Plasma membrane proteins were differentially labeled metabolically, enriched using the colloidal silica pellicle method, and characterized by tandem mass spectrometry. Fifteen proteins were identified with significant (>2) changes, including receptors, adhesion proteins, proteins involved in amino acid uptake, and proteins involved in glucose uptake. From 40 mug of membrane proteins, 3227 unique peptides and 540 proteins were identified.
Rahbar
Amir M
AM
Department of Chemistry and Biochemistry, University of Maryland, College Park Maryland, USA.
Fenselau
Catherine
C
D000970:Antineoplastic Agents / Q000494:pharmacology; D001943:Breast Neoplasms / Q000378:metabolism; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D019008:Drug Resistance, Neoplasm; D004591:Electrophoresis, Polyacrylamide Gel; D015972:Gene Expression Regulation, Neoplastic; D005947:Glucose / Q000493:pharmacokinetics; D006801:Humans; D013058:Mass Spectrometry; D008942:Mitoxantrone / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010455:Peptides / Q000737:chemistry; D004364:Pharmaceutical Preparations; D011506:Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*
D000970:Antineoplastic Agents; D009363:Neoplasm Proteins; D010455:Peptides; D004364:Pharmaceutical Preparations; D011506:Proteins; D020543:Proteome; D008942:Mitoxantrone; D005947:Glucose