[Cyclosporosis: an emergent parasitosis. (II) Microbiological diagnosis through a new staining technique].
OBJECTIVE
Describe a new staining technique for the diagnosis of Cyclospora infection through lab tests. A differential microscopic diagnosis between Cyclospora and Blastocystis hominis is conducted.


METHODS
The research was conducted by using fecal samples referred to a private lab in the city of Lima, Peru. A direct fresh assay was made by using a saline solution and Lugol's solution. Very thin fecal smear were prepared, and a staining technique using gentian violet was applied.


RESULTS
Microphotography of Cyclospora cysts are shown with the fresh assay method and the new staining technique. Moreover the morphological and structural differences between Cyclospora and Blastocystis hominis were shown: the latter is larger, shows poor staining, and has vacuoles.


CONCLUSIONS
The routine use of this technique is recommended for all fecal tests due to its simplicity and low cost, and because it allows a quick diagnosis of this type of infection that has a high prevalence in our country so it may be treated prematurely and specifically.
Burstein Alva
Sonia
S
Facultad de Medicina de la Universidad Nacional Mayor de San Marcos, Lima.
D000818:Animals; D016845:Blastocystis hominis / Q000302:isolation & purification; D004396:Coloring Agents; D021744:Cyclospora / Q000302:isolation & purification*; D021866:Cyclosporiasis / Q000175:diagnosis* / Q000382:microbiology*; D005840:Gentian Violet; D006801:Humans; D010274:Parasitology / Q000379:methods
D004396:Coloring Agents; D005840:Gentian Violet