Determination of donepezil hydrochloride in human and rat plasma, blood and brain microdialysates by HPLC with a short C30 column.
A simple and sensitive HPLC method with fluorescence (FL) detection for determination of donepezil (DP) in plasma and microdialysate samples was developed. A rapid isocratic separation of DP could be achieved by a short C30 column using mobile phases of 25 mM citric acid/50 mM Na2HPO4 (pH 6.0)-CH3CN (73:27%, v/v) containing 3.5 mM sodium 1-octanesulfonate for plasma and H2O-CH3CN-CH3OH (80:17:3%, v/v/v) containing 0.01% acetic acid for microdialysate. The eluate was monitored at 390 nm with an excitation at 325 nm. The detection limits (S/N = 3) of DP for human plasma, rat plasma and rat brain or blood microdialysates were 0.2, 1.0 and 2.1 ng/ml, respectively. Reproducible results could be obtained by using (+/-)-2-[(1-benzyl-piperidine-4-yl)ethyl]-5,6-dimethoxyindan-1-one hydrochloride as an internal standard (IS). The method was successfully applied for monitoring of DP levels in rat plasma, blood and brain microdialysates and patient plasma.
Nakashima
Kenichiro
K
Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Department of Clinical Pharmacy Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. naka-ken@net.nagasaki-u.ac.jp
Itoh
Keiko
K
Kono
Michio
M
Nakashima
Mihoko N
MN
Wada
Mitsuhiro
M
D000818:Animals; D001923:Brain Chemistry; D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077265:Donepezil; D006801:Humans; D007189:Indans / Q000032:analysis* / Q000737:chemistry; D008297:Male; D017551:Microdialysis / Q000379:methods; D008956:Models, Chemical; D010880:Piperidines / Q000032:analysis* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors
D002800:Cholinesterase Inhibitors; D007189:Indans; D010880:Piperidines; D000077265:Donepezil