Analysis of urinary nucleosides. V. Identification of urinary pyrimidine nucleosides by liquid chromatography/electrospray mass spectrometry.
Modified urinary nucleosides are potentially invaluable in cancer diagnosis, as they reflect altered RNA turnovers. High-performance liquid chromatography (HPLC) was combined with full-scan mass spectrometry, tandem mass spectrometry, MS(n) analysis and accurate mass measurements in order to identify pyrimidine nucleosides purified from urine. Potential nucleosides were assessed by their evident UV absorbance in the HPLC chromatogram and then further examined by the various mass spectrometric techniques. In this manner numerous pyrimidine nucleosides were identified in the urine samples from cancer patients including pseudouridine, cytidine, two methylcytidines and an acetylcytidine. Furthermore, a number of novel modified pyrimidine nucleosides were tentatively identified via critical interpretation of the combined mass spectrometric data.
Bond
Alison
A
Biomolecular Analysis Mass Spectrometry (BAMS) Facility, Grove Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.
Dudley
Edward
E
Lemière
Filip
F
Tuytten
Robin
R
El-Sharkawi
Salah
S
Brenton
A Gareth
AG
Esmans
Eddy L
EL
Newton
Russell P
RP
D014408:Biomarkers, Tumor / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis* / Q000652:urine*; D011741:Pyrimidine Nucleosides / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D016482:Urinalysis / Q000379:methods*
D014408:Biomarkers, Tumor; D011741:Pyrimidine Nucleosides