Development of a triplex real-time PCR assay for detection of Panton-Valentine leukocidin toxin genes in clinical isolates of methicillin-resistant Staphylococcus aureus.
Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA.
McDonald
Ryan R
RR
Provincial Laboratory, Saskatchewan Health, 3211 Albert Street, Regina, Saskatchewan S4S 5W6, Canada. rmcdonal@health.gov.sk.ca
Antonishyn
Nick A
NA
Hansen
Toni
T
Snook
Laelie A
LA
Nagle
Evelyn
E
Mulvey
Michael R
MR
Levett
Paul N
PN
Horsman
Greg B
GB
D001427:Bacterial Toxins; D017714:Community-Acquired Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D005098:Exotoxins; D006801:Humans; D007956:Leukocidins; D016106:Methicillin Resistance; D016133:Polymerase Chain Reaction / Q000379:methods*; D018461:Soft Tissue Infections / Q000382:microbiology; D013207:Staphylococcal Skin Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin