Multi-peak phenomenon of insect cell infection with baculovirus at low multiplicity of infection.
In this communication we report the infection of armyworm Spodoptera frugiperda IPLB-Sf-21 cells with Anticarsia gemmatalis multicapsid nucleopolyhedrovirus at low multiplicity of infection (MOI). The temporal variation of the extra-cellular virus and of the unstained cell was followed. The series of peaks in the virus concentration and the unstained cells count were used in order to infer the dynamic mechanism of the infection at low MOI. This mechanism can be used as the basis for the future formulation of a mathematical model of the process.
Zhang
You-Hong
YH
Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, China. youhong64@yahoo.com.cn
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D017924:Nucleopolyhedroviruses / Q000254:growth & development*; D010053:Ovary / Q000821:virology; D018411:Spodoptera / Q000378:metabolism / Q000821:virology*; D013997:Time Factors; D019562:Viral Load