[Physiological comportment and in vivo sensitivity of Sporothrix schenckii isolates maintained for 18 years by two preservation methods].
We compared two methods for the preservation of fungi, Castellani's method and repeated passage in Sabouraud medium-agar, on five isolates of Sporothrix schenckii that were preserved for 18 years at room temperature by both procedures. They were evaluated for viability of the strains, growth rate, morphological and physiological characteristics, and in vitro sensitivity to iodide, itraconazole, terbinafine and posaconazole. 100% viability was observed in all of the isolates, with slower growth rate on strains preserved in water compared to strains periodically re-cultured. The typical morphological feature of these fungi was preserved by both methodologies. With regard to enzymatic activity, both groups gave urease reactions and were beta glucosidase-positive. Nevertheless, complete inhibition of the capacity to hydrolyse starch was observed only on the isolates preserved in water. This group also was more sensitive to potassium iodide at a concentration of 10 microM in the in vitro sensitivity tests.
Mendoza
Mireya
M
Laboratorio de Micología, Instituto de Biomedicina, Apdo Postal 4043, San José, Caracas 1010A, Venezuela. mmendoz@telcet.net.ve
Alvarado
Primavera
P
Díaz de Torres
Elvia
E
Lucena
Lilianyel
L
de Albornoz
María C
MC
D000362:Agar; D000935:Antifungal Agents / Q000494:pharmacology; D025141:Drug Resistance, Fungal; D005947:Glucose; D017964:Itraconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009172:Mycology / Q000379:methods*; D009281:Naphthalenes / Q000494:pharmacology; D011193:Potassium Iodide / Q000494:pharmacology; D011309:Preservation, Biological / Q000379:methods*; D013175:Sporothrix / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D013213:Starch / Q000378:metabolism; D000077291:Terbinafine; D013997:Time Factors; D014230:Triazoles / Q000494:pharmacology; D014867:Water
D000935:Antifungal Agents; D009281:Naphthalenes; D014230:Triazoles; D014867:Water; D011193:Potassium Iodide; D017964:Itraconazole; C101425:posaconazole; D000362:Agar; D013213:Starch; D000077291:Terbinafine; D005947:Glucose