Phospholipid interactions protect the milk allergen alpha-lactalbumin from proteolysis during in vitro digestion.
Interactions with food components may alter the resistance of food proteins to digestion, a property thought to play an important role in determining allergenic properties. The kinetics of breakdown of the bovine milk allergen alpha-lactalbumin during in vitro gastrointestinal digestion was found to be altered by interactions with physiologically relevant levels of phosphatidylcholine (PC), a surfactant that is abundant both in milk and is actively secreted by the stomach. Breakdown during gastric digestion was slowed in the presence of PC and accompanied by small alterations in the profile of resulting peptides, with little effect being observed during subsequent duodenal digestion. alpha-Lactalbumin was found to unfold at gastric (acid) pH, giving a CD spectrum similar to that obtained for the partially folded state it is known to adopt at pH values below its isoelectric point. Fluorescence polarization studies performed at low pH indicated that this partially unfolded form of the protein was able to penetrate into the PC vesicles. These interactions are probably responsible for the slowing of gastric digestion by reducing the accessibility of the protein to pepsin. These findings show that interactions with other food components, such as lipids, may alter the rate of breakdown of food proteins in the gastrointestinal tract. It underlines the importance of the food matrix in affecting patterns of food allergen digestion and hence presentation to the immune system and that in vitro digestion systems used for assessing digestibility of allergens must take account of surfactants.
Moreno
F Javier
FJ
Institute of Food Research, Norwich Research Park, Colney Lane, Norwich NR4 7UA, United Kingdom. j.moreno@ifi.csic.es
Mackie
Alan R
AR
Mills
E N Clare
EN
D000485:Allergens / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004063:Digestion; D004347:Drug Interactions; D004386:Duodenum; D066298:In Vitro Techniques; D007768:Lactalbumin / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism; D008892:Milk / Q000276:immunology*; D010179:Pancreas / Q000201:enzymology; D010434:Pepsin A / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D013270:Stomach; D013552:Swine
D000485:Allergens; D010713:Phosphatidylcholines; D007768:Lactalbumin; D008049:Lipase; D010447:Peptide Hydrolases; D010434:Pepsin A