title: Cytotoxic effects of the root extracts of Eurycoma longifolia Jack.
abstract: The methanol, n-butanol, chloroform and water extracts obtained from the root of Eurycoma longifolia Jack were assayed using methylene blue assay to evaluate its cytotoxic effect against KB, DU-145, RD, MCF-7, CaOV-3, MDBK cell lines. The results showed that all the root extracts except the water extract of E. longifolia produced significant cytotoxic effect on these cell lines. However, no significant cytotoxic effect was detected on MDBK (kidney) normal cell line. 9-methoxycanthin-6-one, an alkaloid, was detected in each extract with different intensities by reversed-phase high performance liquid chromatography.
authors.affiliation: Biotechnology Division, Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia. hanan@frim.gov.my
authors.forename: M Y
authors.initials: MY
authors.lastname: Nurhanan
authors.forename: L P
authors.initials: LP
authors.lastname: Azimahtol Hawariah
authors.forename: A
authors.initials: A
authors.lastname: Mohd Ilham
authors.forename: M A
authors.initials: MA
authors.lastname: Mohd Shukri
mesh_terms: D002243:Carbolines / Q000032:analysis / Q000633:toxicity; D045744:Cell Line, Tumor / Q000187:drug effects*; D035701:Eurycoma; D006801:Humans; D008751:Methylene Blue; D010936:Plant Extracts / Q000633:toxicity*; D018517:Plant Roots / Q000737:chemistry; D007769:Lactams
chemical_list: D002243:Carbolines; D008751:Methylene Blue; D010936:Plant Extracts; C459628:9-methoxycanthin-6-one; D007769:Lactams