title: Survey of human immunodeficiency virus (HIV)-seropositive patients with mycobacterial infection in Japan.
abstract: OBJECTIVE
  To assess DNA polymorphisms in mycobacterial isolates obtained from human immunodeficiency virus (HIV)-seropositive patients with tuberculosis in Japan from 1996 to 2003.
  
  
  METHODS
  Restriction fragment length polymorphisms (RFLP) from Mycobacterium tuberculosis and Mycobacterium avium isolates obtained from individual seropositive patients with tuberculosis (n=78) were analysed with the use of IS6110 and (CGG)(5) or IS1245 and IS1311, respectively, as markers. As a control, the same procedures were applied to isolates from HIV-seronegative tuberculosis patients (n=87).
  
  
  RESULTS
  Of 86 mycobacterial strains, M. tuberculosis, M. avium and Mycobacterium chelonae were identified in 48 (55.8%), 36 (41.9%) and 2 (2.3%) isolates, respectively. The obtained RFLP patterns of M. tuberculosis isolates from both the HIV-seropositive and -seronegative groups were variable, suggesting no obvious clustering among the isolates. Similar results were obtained in isolates of M. avium.
  
  
  CONCLUSIONS
  This is the first report on the molecular epidemiology of Mycobacterium spp. isolated from HIV-seropositive patients in Japan. The results indicate that no particular clones of M. tuberculosis or M. avium prevail in HIV-seropositive patients in Japan. Further monitoring of mycobacterial infection associated with HIV infection in Japan should be continued.
authors.affiliation: International Medical Center of Japan, Toyama 1-21-1, Shinjuku-ku, Tokyo 162-8655, Japan.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Tomoko
authors.initials: T
authors.lastname: Fujino
authors.forename: Namiko
authors.initials: N
authors.lastname: Mori
authors.forename: Jun-ichiro
authors.initials: J
authors.lastname: Sekiguchi
authors.forename: Emiko
authors.initials: E
authors.lastname: Toyota
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Saruta
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: Yuka
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ajisawa
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagai
authors.forename: Makoto
authors.initials: M
authors.lastname: Takahara
authors.forename: Hideo
authors.initials: H
authors.lastname: Saka
authors.forename: Takuma
authors.initials: T
authors.lastname: Shirasaka
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Makiko
authors.initials: M
authors.lastname: Kiyosuke
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Koga
authors.forename: Shinichi
authors.initials: S
authors.lastname: Oka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kimura
authors.forename: Toru
authors.initials: T
authors.lastname: Mori
authors.forename: Tadatoshi
authors.initials: T
authors.lastname: Kuratsuji
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D016172:DNA Fingerprinting; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000453:epidemiology*; D018023:HIV Seronegativity; D006679:HIV Seropositivity; D006306:Health Surveys; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009164:Mycobacterium Infections / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology; D009162:Mycobacterium avium / Q000235:genetics* / Q000302:isolation & purification; D016926:Mycobacterium chelonae / Q000235:genetics / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence