title: In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate.
abstract: Recently, regular and white mineral trioxide aggregate (MTA) are being used in Dentistry as retrofilling materials. Genotoxicity and cytotoxicity tests form an important part of cancer research and risk assessment of potential carcinogens. Thus, the goal of this study was to examine the genotoxicity and cytotoxicity of regular and white MTA in vitro by the single cell gel (comet) assay and trypan blue exclusion test, respectively. Mouse lymphoma cells were exposed to two presentation forms of MTA at final concentrations ranging from 1 to 1,000 microg/mL for 3 h at 37 degrees C. The results showed that both compounds tested did not produce genotoxic effects at all concentrations evaluated. Likewise, no statistically significant differences (p > 0.05) were observed in cytotoxicity. Taken together, our results suggest that regular and white MTA are not genotoxins and are not able to interfere in cellular viability as assessed by single cell gel (comet) assay and trypan blue assay, respectively.
authors.affiliation: Center for Genotoxin and Carcinogen Evaluation, TOXICAN, Department of Pathology, School of Medicine of Botucatu, São Paulo State University.
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
authors.forename: Mariza Akemi
authors.initials: MA
authors.lastname: Matsumoto
authors.forename: Marco Antonio Húngaro
authors.initials: MA
authors.lastname: Duarte
authors.forename: Mariangela Esther Alencar
authors.initials: ME
authors.lastname: Marques
authors.forename: Daisy Maria Favero
authors.initials: DM
authors.lastname: Salvadori
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D017610:Calcium Compounds / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects*; D020552:Comet Assay; D004249:DNA Damage; D004338:Drug Combinations; D007940:Leukemia L5178 / Q000235:genetics; D008223:Lymphoma / Q000235:genetics; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D009152:Mutagenicity Tests; D010087:Oxides / Q000633:toxicity*; D012184:Retrograde Obturation; D017640:Silicates / Q000633:toxicity*; D014343:Trypan Blue
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D017640:Silicates; C086631:mineral trioxide aggregate; D014343:Trypan Blue