Fusion-based strategies to identify genes involved in degradation of a specific substrate.
Fusion proteins have been used in many instances to allow genetic screening for genes required for the degradation of a specific substrate. This straightforward, yet powerful, approach can be applied in many circumstances to facilitate gene characterization and discovery. Some general principles are discussed and then several successful uses of these tactics are described in detail.
Hampton
Randolph Y
RY
UCSD Division of Biologcal Sciences, La Jolla, California, USA.
D050939:Gene Fusion; D006868:Hydrolysis; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D012097:Repressor Proteins