title: Characterisation of the phenolic profile of Boerhaavia diffusa L. by HPLC-PAD-MS/MS as a tool for quality control.
abstract: Phenolic acids and flavonols of nine leaf and three root samples of Boerhaavia diffusa L., collected at different locations and subjected to several drying procedures, were characterised by reversed-phase HPLC-PAD-ESI/MS for the first time. Ten phenolic compounds were identified: 3,4-dihydroxy-5-methoxycinnamoyl-rhamnoside, quercetin 3-O-rhamnosyl(1-->6)galactoside (quercetin 3-O-robinobioside), quercetin 3-O-(2"-rhamnosyl)-robinobioside, kaempferol 3-O-(2"-rhamnosyl)-robinobioside, 3,5,4'-trihydroxy-6,7-dimethoxyflavone 3-O-galactosyl(1-->2)glucoside [eupalitin 3-O-galactosyl(1-->2)glucoside], caffeoyltartaric acid, kaempferol 3-O-robinobioside, eupalitin 3-O-galactoside, quercetin and kaempferol. Quantification was achieved by HPLC-PAD and two phenolic patterns were found for the leaves, in which quercetin 3-O-robinobioside or quercetin 3-O-(2"-rhamnosyl)-robinobioside was the major compound. Caffeoyltartaric acid was only present in the root material where it represented the main phenolic constituent. The results obtained demonstrated that the geographical origin (particularly the nature of the soil), but not the drying process, influences the phenolic composition.
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), Campus Universidade Espinardo, Murcia, Spain.
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: Carla
authors.initials: C
authors.lastname: Sousa
authors.forename: Mulangu
authors.initials: M
authors.lastname: Justin
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
authors.forename: Rafael
authors.initials: R
authors.lastname: Llorach
authors.forename: Alírio
authors.initials: A
authors.lastname: Rodrigues
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Seabra
authors.forename: Anabela
authors.initials: A
authors.lastname: Leitão
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D039281:Nyctaginaceae / Q000737:chemistry*; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010636:Phenols