Differential expression of manganese superoxide dismutase sequence variants in near isogenic lines of wheat during cold acclimation.
Numerous sequence variants of wheat (Triticum aestivum L.) manganese superoxide dismutase (MnSOD) genes have been found. Quantitative real-time PCR was used to measure the expression levels of three MnSOD genes distinguished by a variable amino acid, and three genes distinguished by sequence variation in the 3' untranslated region (3' UTR), in wheat plants grown at 20 degrees C and cold-acclimated for 1-4 weeks at 2 degrees C. The amino acid variants did not differ significantly in expression levels, however, differential expression of genes differing in the 3' UTR was observed. Diploid wheat-related species also carried sequence variants of MnSOD, with differing levels of expression, suggesting diversification of the MnSOD gene family occurred prior to the polyploidization events of hexaploid wheat.
Baek
Kwang-Hyun
KH
Department of Crop and Soil Sciences, 210 Johnson Hall, Washington State University, Pullman, WA 99164-6420, USA.
Skinner
Daniel Z
DZ
D020413:3' Untranslated Regions / Q000235:genetics; D000064:Acclimatization; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D003080:Cold Temperature; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014908:Triticum / Q000201:enzymology* / Q000235:genetics*
D020413:3' Untranslated Regions; D000596:Amino Acids; D012333:RNA, Messenger; D013482:Superoxide Dismutase