Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora.
A metagenome expression library of bulk DNA extracted from the rumen content of a dairy cow was established in a phage lambda vector and activity-based screening employed to explore the functional diversity of the microbial flora. Twenty-two clones specifying distinct hydrolytic activities (12 esterases, nine endo-beta-1,4-glucanases and one cyclodextrinase) were identified in the library and characterized. Sequence analysis of the retrieved enzymes revealed that eight (36%) were entirely new and formed deep-branched phylogenetic lineages with no close relatives among known ester- and glycosyl-hydrolases. Bioinformatic analyses of the hydrolase gene sequences, and the sequences and contexts of neighbouring genes, suggested tentative phylogenetic assignments of the rumen organisms producing the retrieved enzymes. The phylogenetic novelty of the hydrolases suggests that some of them may have potential for new applications in biocatalysis.
Ferrer
Manuel
M
Department of Microbiology, GBF- German Research Centre for Biotechnology, Mascheroder Weg 1, 38124 Braunschweig, Germany. m.ferrer@icp.csic.es
Golyshina
Olga V
OV
Chernikova
Tatyana N
TN
Khachane
Amit N
AN
Reyes-Duarte
Dolores
D
Santos
Vitor A P Martins Dos
VA
Strompl
Carsten
C
Elborough
Kieran
K
Jarvis
Graeme
G
Neef
Alexander
A
Yakimov
Michail M
MM
Timmis
Kenneth N
KN
Golyshin
Peter N
PN
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D044822:Biodiversity; D002417:Cattle; D005260:Female; D005656:Fungal Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D005800:Genes, Fungal; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics; D006867:Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D009520:New Zealand; D012417:Rumen / Q000382:microbiology* / Q000469:parasitology
D001426:Bacterial Proteins; D005656:Fungal Proteins; D015801:Helminth Proteins; D006867:Hydrolases