[Kidney accumulation of yellow fluorescent protein after its absorption in the rat intestine].
Yellow fluorescent protein (3.6 ng) was administered through a catheter into the Wistar rat intestine lumen. By the method of confocal microscopy it has been established that as soon as in 3 min this protein appears in the epithelial cells of ileum, is absorbed into the blood and accumulated in cells of the nephron proximal segment, not being revealed in the liver, though. The protein accumulation in kidneys continues for several hours. The yellow fluorescent protein is homogeneously distributed in enterocytes, while in epithelial cells of the proximal tubule this protein is localized in vesicles. The data obtained indicate absorption of non-degraded yellow fluorescent protein in the intestine and role of kidney in metabolism not only of endogenous, but also of exogenous proteins.
Burmakin
M V
MV
Seliverstova
E V
EV
Natochin
Iu V
IuV
D000042:Absorption; D000818:Animals; D020895:Enterocytes / Q000166:cytology / Q000502:physiology*; D005260:Female; D007082:Ileum / Q000166:cytology / Q000502:physiology*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000502:physiology*; D008164:Luminescent Proteins / Q000008:administration & dosage / Q000378:metabolism*; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar
D008164:Luminescent Proteins