Detection and serotyping of Streptococcus pneumoniae from nasopharyngeal samples by PCR-based multiplex assay.
We developed a multiplex PCR-based methodology for nasopharyngeal samples maintained in egg thioglycolate antibiotic and skim milk-tryptone-glucose-glycerol media to identify and serotype the most important serotypes of Streptococcus pneumoniae that cause invasive disease in children. This technique can be used to study the epidemiology of pneumococcal colonization and the effect of conjugate vaccines.
Moreno
Jaime
J
Instituto Nacional de Salud, Avenida calle 26 No. 51-60, Bogotá, Colombia. ecastaneda@ins.gov.co
Hernández
Elkin
E
Sanabria
Olga
O
Castañeda
Elizabeth
E
D001431:Bacteriological Techniques; D002675:Child, Preschool; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000382:microbiology*; D011008:Pneumococcal Infections / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D004269:DNA, Bacterial