Alcohol-induced bone degradation and its early detection in the alcohol-fed castrated rats.
The objective of this study was to examine alcohol-induced changes of bone in hormone-deficient males using the developed method. In the process of bone resorption, type I collagen crosslinking molecules, pyridinoline (PYD), are released into the circulation and cleared by the kidneys. (2)H(2)O as a tracer has been applied to measure the synthesis rates of slow-turnover proteins and successfully applied to bone collagen synthesis in our hormone deficiency rats. This study demonstrated for the first time, the early changes of the femur bone degradation in hormone-deficient male individuals, more influenced by alcohol through histopathological study, serum PYD assay, and (2)H(2)O labeling. We also observed that serum PYD was a sensitive pathological marker of bone degradation in castrated osteoporosis males and the unique features of (2)H(2)O labeling to measure the bone turnover collagen synthesis rates were excellent markers of bone degradation and aging.
Do
Sun Hee
SH
Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, #1370 Sangyeok-dong, Buk-gu, Daegu 702-701, Republic of Korea.
Jeong
Won Il
WI
Jeong
Da Hee
DH
Ki
Mi-Ran
MR
Lee
In-Seon
IS
Kwak
Dong-Mi
DM
Kim
Tae Hwan
TH
Kim
Yoo Kyeong
YK
Kim
Soon-Bok
SB
Jeong
Kyu-Shik
KS
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001862:Bone Resorption / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D024042:Collagen Type I / Q000378:metabolism; D003903:Deuterium; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D005269:Femur / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009919:Orchiectomy; D051381:Rats; D017208:Rats, Wistar; D014867:Water
D000596:Amino Acids; D024042:Collagen Type I; D014867:Water; D000431:Ethanol; C015484:pyridinoline; D003903:Deuterium