[The relationship between the concentration of TGF-beta1 in serum and its gene polymorphisms in CWP].
OBJECTIVE
To study the relationship between the concentration of TGF-beta1, in serum and its gene polymorphisms in CWP.


METHODS
101 cases of coal worker's pneumoconiosis (CWP)were selected. And the peripheral vein blood samples were collected from them. The concentration of TGF-beta1 of their serum were detected with the TGF-beta1 ELISA kit. And their TGF-beta1 gene -509 site polymorphisms were analyzed with the polymerse chain reaction-restriction fragment length polymorphisms (PCR-RFLP) techniques.


RESULTS
The mean value of TGF-beta1, concentration in serum of T/T, C/T and C/C gene type group were 90 ml, 60 ml and 55.960 +/- 28.833 ng/ ml respectively. Significant differences were observed within the three groups (P<0.05). The differences of the mean value of TGF-beta1, concentration in serum in various work years groups, age groups and in the groups with different stage of pneumoconiosis were not significant (P > 0.05).


CONCLUSIONS
The correlative relationship between the concentration of transforming growth factor I (TGF-beta1) in serum and its gene -509 site polymorphisms might be occurring.
Yao
Wu
W
Department of Occupational Health, West China School of Public Health, Sichuan University, Chengdu 614401, China.
Wang
Zhi-Ming
ZM
Wang
Mian-Zhen
MZ
Hao
Chang-Fu
CF
Wang
Na
N
D003032:Coal Mining; D006801:Humans; D008297:Male; D008875:Middle Aged; D011009:Pneumoconiosis / Q000235:genetics* / Q000378:metabolism; D017354:Point Mutation; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D016212:Transforming Growth Factor beta / Q000097:blood* / Q000235:genetics*; D053773:Transforming Growth Factor beta1
C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1