cDNA cloning and expression of ferritin heavy chain 1, ferritin heavy chain 2 and hemoglobin genes from the fire-bellied frog Bombina orientalis.
We cloned two Bombina orientalis ferritin heavy chains (ferritin heavy chains 1 and 2) and one hemoglobin beta-chain gene from a B. orientalis oviduct cDNA library, and the length of transcripts was 882, 858 and 611 bp encoding 177, 177 and 148 aa, respectively. B. orientalis ferritin heavy chain genes showed high similarity to those of amphibia (88-93%), mammals (70-71%), and fishes (70-72%), and the hemoglobin beta-chain gene showed moderate similarity to amphibian (65-68%) and mammalian (54-57%) hemoglobin beta-chain genes, respectively. Based on phylogenetic analysis, the genes were clustered to the same clade in amphibia. The two B. orientalis ferritin heavy chain genes showed different tissue-specific gene expression patterns. Thus, ferritin heavy chain 1 gene was highly expressed in intestine and oviduct but ferritin heavy chain 2 gene was ubiquitously expressed in most of the examined tissues. The hemoglobin beta-chain gene was more highly expressed in liver than in oviduct. These findings indicate that the genes may play different roles in different tissues. In this paper, we discuss the basic characteristics of B. orientalis ferritin heavy chain genes and hemoglobin beta-chain gene.
Kim
Min-Sun
MS
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, South Korea.
Kim
Il-Chan
IC
Lee
Young-Mi
YM
Jung
Sang-Oun
SO
Seo
Jung Soo
JS
Yoon
Yong-Dal
YD
Lee
Jae-Seong
JS
D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D005293:Ferritins / Q000096:biosynthesis / Q000235:genetics*; D015723:Gene Library; D006454:Hemoglobins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010057:Oviducts / Q000378:metabolism; D010802:Phylogeny; D021122:Protein Subunits / Q000096:biosynthesis / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D006454:Hemoglobins; D021122:Protein Subunits; D005293:Ferritins