Measuring cell viscoelastic properties using a force-spectrometer: influence of protein-cytoplasm interactions.
Cell adhesive and rheological properties play a very important role in cell transmigration through the endothelial barrier, in particular in the case of inflammation (leukocytes) or cancer metastasis (cancer cells). In order to characterize cell viscoelastic properties, we have designed a force spectrometer (AFM) which can stretch cells thereby allowing measurement of their rheological properties. This custom-made force spectrometer allows two different visualizations, one lateral and one from below. It allows investigation of the effects of rheology involved during cell stretching. To test the ability of our system to characterize such viscoelastic properties, ICAM-1 transfected CHO cells were analyzed. Two forms of ICAM-1 were tested; wild type ICAM-1, which can interact with the cytoskeleton, and a mutant form which lacks the cytoplasmic domain, and is unable to associate with the cytoskeleton. Stretching experiments carried out on these cells show the formation of long filaments. Using a previous model of filament elongation, we could determine the viscoelastic properties of a single cell. As expected, different viscoelastic components were found between the wild type and the mutant, which reveal that the presence of interactions between ICAM-1 and the cytoskeleton increases the stiffness of the cell.
Canetta
Elisabetta
E
Université Grenoble I, Laboratoire de Spectrométrie Physique, Grenoble, France.
Duperray
Alain
A
Leyrat
Anne
A
Verdier
Claude
C
D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D002468:Cell Physiological Phenomena; D006224:Cricetinae; D003599:Cytoskeleton / Q000378:metabolism / Q000502:physiology*; D004548:Elasticity; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D040542:Mechanotransduction, Cellular / Q000502:physiology; D018625:Microscopy, Atomic Force / Q000379:methods; D012212:Rheology; D013314:Stress, Mechanical; D014783:Viscosity
D018799:Intercellular Adhesion Molecule-1