Mouse strain dependence of lung tissue mechanics: role of specific extracellular matrix composition.
This study analyses the differences between C57BL/10 and BALB/c mice in lung tissue micromechanical behaviour and whether specific histological characteristics are related to the mechanical profile. C57BL/10 and BALB/c subpleural lung strips were submitted to multisinusoidal deformation with frequencies ranging between 0.2 and 3.1 Hz. Tissue resistance (R), elastance (E), and hysteresivity (eta) at each frequency were determined before and 30s, 1, 2, and 3 min after acetylcholine (ACh) treatment. BALB/c mice showed higher E and R, at baseline, as well as greater amount of collagen and elastic fibres, and alpha-actin than C57BL/10 mice. However, E, R, and eta augmented with the same magnitude after ACh treatment in both strains. Baseline R was correlated with collagen fibre content and with the volume proportion of alpha-actin, while E was correlated with elastic and collagen fibres, and alpha-actin contents. In conclusion, BALB/c and C57BL/10 mice present distinct tissue mechanical properties that are accompanied by specific extracellular matrix composition and contractile structures.
Faffe
Debora S
DS
Laboratory of Respiration Physiology, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Centro de Ciências da Saúde, Ilha do Fundão, Brazil.
D'Alessandro
Elizabeth S
ES
Xisto
Debora G
DG
Antunes
Mariana A
MA
Romero
Pablo V
PV
Negri
Elnara M
EM
Rodrigues
Nilza R D
NR
Capelozzi
Vera L
VL
Zin
Walter A
WA
Rocco
Patricia R M
PR
D000109:Acetylcholine / Q000494:pharmacology; D000199:Actins / Q000378:metabolism; D000818:Animals; D001696:Biomechanical Phenomena / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004547:Elastic Tissue / Q000187:drug effects / Q000502:physiology; D004548:Elasticity / Q000187:drug effects; D005109:Extracellular Matrix / Q000187:drug effects / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D008168:Lung / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008170:Lung Compliance / Q000187:drug effects; D019563:Mechanics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009130:Muscle, Smooth / Q000378:metabolism; D013045:Species Specificity; D013314:Stress, Mechanical
D000199:Actins; D000109:Acetylcholine