Bioaccumulation of copper by Trichoderma viride.
Studies were carried out on interaction of Trichoderma viride with copper and reports bioaccumulation as a mechanism of copper tolerance during growth. There was a marked increase in the lag phase of the growth, which was concentration dependent. At a concentration of 100 mg/L of CuCl2.2H2O, 81% of Cu(II) were removed by 3.4 g/L of the biomass in 72 h. The process was temperature and pH dependent. The maximum copper bioaccumulation occurred at 30 degrees C, pH 5.0. Metabolic inhibitors such as sodium azide (NaN3) and 2,4-dinitrophenol (2,4-DNP) drastically reduced the extent of Cu(II) bioaccumulation. Electron microscopy and cell fractionation studies revealed that 70-80% of copper was present as a layer on the cell wall surface.
Anand
Purnima
P
Department of Microbiology, University of Delhi, South Campus, Benito Juarez Road, New Delhi 110 021, India.
Isar
Jasmine
J
Saran
Saurabh
S
Saxena
Rajendra Kumar
RK
D019297:2,4-Dinitrophenol / Q000633:toxicity; D018533:Biomass; D019149:Bioreactors; D002458:Cell Fractionation; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D002614:Chelating Agents / Q000494:pharmacology; D003300:Copper / Q000493:pharmacokinetics* / Q000494:pharmacology; D003470:Culture Media / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D025282:Mycelium / Q000737:chemistry; D019810:Sodium Azide / Q000633:toxicity; D012988:Soil Microbiology; D013696:Temperature; D014242:Trichoderma / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D014475:Uncoupling Agents / Q000633:toxicity
D002614:Chelating Agents; D003470:Culture Media; D014475:Uncoupling Agents; D004533:Egtazic Acid; D003300:Copper; D019810:Sodium Azide; D019297:2,4-Dinitrophenol; C029892:cupric chloride