Production of vanillin by metabolically engineered Escherichia coli.
E. coli was metabolically engineered to produce vanillin by expression of the fcs and ech genes from Amycolatopsis sp. encoding feruloyl-CoA synthetase and enoyl-CoA hydratase/aldolase, respectively. Vanillin production was optimized by leaky expression of the genes, under the IPTG-inducible trc promoter, in complex 2YT medium. Supplementation with glucose, fructose, galactose, arabinose or glycerol severely decreased vanillin production. The highest vanillin production of 1.1 g l(-1) was obtained with cultivation for 48 h in 2YT medium with 0.2% (w/v) ferulate, without IPTG and no supplementation of carbon sources.
Yoon
Sang-Hwal
SH
Department of Food Science & Nutrition, Gyeongsang National University, 660-701, Jinju, Korea.
Li
Cui
C
Kim
Ju-Eun
JE
Lee
Sook-Hee
SH
Yoon
Ji-Young
JY
Choi
Myung-Suk
MS
Seo
Weon-Taek
WT
Yang
Jae-Kyung
JK
Kim
Jae-Yeon
JY
Kim
Seon-Won
SW
D000192:Actinomycetales / Q000235:genetics* / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D001547:Benzaldehydes / Q000302:isolation & purification* / Q000378:metabolism*; D004746:Enoyl-CoA Hydratase / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*
D001547:Benzaldehydes; C100058:vanillin; D000445:Aldehyde Oxidoreductases; C023101:feruloyl-coenzyme A reductase; D004746:Enoyl-CoA Hydratase