Antagonism between olive oil phenolics and nitric oxide on lymphomonocyte cytosolic calcium.
Some biological actions of olive oil phenolics (inhibition of platelet aggregation, decrease of LDL-oxidation, inhibition of bacterial growth and hypertensive action) have been attributed to NOS stimulation in endothelial cells through an increase of cytosolic calcium, notwithstanding the scavenging activity of phenolics on NO and superoxide. In this paper, we determine the concentration of cytosolic calcium in human lymphomonocytes incubated with high concentrations of NO-donors (CysNO) and we evaluate the effects of olive oil phenolics on this parameter. CysNO induces a marked decrease of cytosolic calcium; both olive oil phenolics oppose this action of CysNO. The effects of phenolics and CysNO are independent and additive.
Palmerini
Carlo A
CA
Dipartimento di Medicina Interna, Università di Perugia, Via del Giochetto, 06127, Perugia, Italy. crlpal@unipg.it
Carlini
Enrico
E
Saccardi
Carla
C
Servili
Maurizio
M
Montedoro
Gianfrancesco
G
Arienti
Giuseppe
G
D015098:3-Methoxy-4-hydroxyphenylethanol / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects*; D003600:Cytosol / Q000378:metabolism*; D004347:Drug Interactions; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism*; D009543:Nifedipine / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology*
D020030:Nitric Oxide Donors; D000069463:Olive Oil; D010938:Plant Oils; D015098:3-Methoxy-4-hydroxyphenylethanol; D009569:Nitric Oxide; D009543:Nifedipine