[The role of the Yersinia plasmid of calcium-dependency in the formation of the plague immunity].
It was shown that viable variants of the Y. enterocolitica microorganisms containing the plasmid of Ca-dependency (native one or from EV/ I Y. pestis cells) were more immunogenic to plague-infected guinea pigs than isogenic non-plasmid variant. As a result of studying the protective properties of the bacteria Y. enterocolitica sub-cellular fractions it was determined that the largest quantity of immunogen per unit of protein was accumulated in the cultures of bacteria's plasmid variant. The aggregate of the obtained experimental data suggested that there was a non-identified protective antigen (or antigens) for guinea pigs to be encoded by chromosome's DNA. Besides, the Ca-dependency plasmid gene products take part in its excretion and, as the factors of pathogenicity, provide survivability of the Y. enterocolitica microorganisms in the host organism, increasing thus the immunogen accumulation in it.
Byvalov
A A
AA
Darmov
I V
IV
Marakulin
I V
IV
Obrosova
A G
AG
Pogorel'skiĭ
I P
IP
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry; D002118:Calcium / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006168:Guinea Pigs; D008297:Male; D010930:Plague / Q000276:immunology; D010931:Plague Vaccine / Q000235:genetics / Q000276:immunology; D010957:Plasmids / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D052899:Pore Forming Cytotoxic Proteins; D015009:Yersinia Infections / Q000276:immunology*; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C043908:LcrV protein, Yersinia; D010931:Plague Vaccine; D052899:Pore Forming Cytotoxic Proteins; D002118:Calcium