Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region.
The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome.
Luo
Meizhong
M
Department of Plant Sciences, Arizona Genomics Institute, University of Arizona, Tucson, AZ 85721, USA.
Yu
Yeisoo
Y
Kim
Hyeran
H
Kudrna
Dave
D
Itoh
Yuichiro
Y
Agate
Robert J
RJ
Melamed
Esther
E
Goicoechea
José L
JL
Talag
Jayson
J
Mueller
Christopher
C
Wang
Wenming
W
Currie
Jennifer
J
Sisneros
Nicholas B
NB
Wing
Rod A
RA
Arnold
Arthur P
AP
D000818:Animals; D001483:Base Sequence; D001522:Behavior, Animal / Q000502:physiology; D002645:Chickens / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D046369:Finches / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D011944:Receptors, Androgen / Q000235:genetics*
D011944:Receptors, Androgen