Using redox potential to detect microbial activities during clavulanic acid biosynthesis in Streptomyces clavuligerus.
Production of clavulanic acid (CA) by Streptomyces clavuligerus in a shake-flask culture increased from 92 to 180 mg l(-1) with an increased O2 transfer efficiency (0.039 --> 0.058 s(-1)), which maintained the redox potential values above -250 mV. Compared with traditional measures, such as dissolved O2 concentration and respiratory activity, the redox potential can easily be determined and correlates closely with CA production. It can therefore be used to monitor microbial activities during biosyntheses of secondary metabolites.
Lin
Yun-Huin
YH
Energy & Resources Laboratories, Industrial Technology Research Institute, Hsinchu, 310, Taiwan.
Hwang
Sz-Chwun John
SC
Gong
Jian Tong
JT
Wu
Jane-Yii
JY
Chen
Kuo-Cheng
KC
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D019818:Clavulanic Acid / Q000096:biosynthesis*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D011048:Polarography / Q000379:methods*; D013302:Streptomyces / Q000378:metabolism*
D019818:Clavulanic Acid; D010100:Oxygen