title: Synergistic effect of enterocin AS-48 in combination with outer membrane permeabilizing treatments against Escherichia coli O157:H7.
abstract: OBJECTIVE
  To determine the effects of outer membrane (OM) permeabilizing agents on the antimicrobial activity of enterocin AS-48 against Escherichia coli O157:H7 CECT 4783 strain in buffer and apple juice.
  
  
  RESULTS
  We determined the influence of pH, EDTA, sodium tripolyphosphate (STPP) and heat on E. coli O157:H7 CECT 4783 sensitivity to enterocin AS-48 in buffer and in apple juice. Enterocin AS-48 was not active against intact cells of E. coli O157:H7 CECT 4783 at neutral pH. However, cells sublethally injured by OM permeabilizing agents (EDTA, STPP, pH 5, pH 8.6 and heat) became sensitive to AS-48, decreasing the amount of bacteriocin required for inhibition of E. coli O157:H7 CECT 4783.
  
  
  CONCLUSIONS
  The results presented indicate that enterocin AS-48 could potentially be applied with a considerably wider range of protective agents, such as OM permeabilizing agents, with increased efficacy in inhibiting E. coli O157:H7.
  
  
  CONCLUSIONS
  Results from this study support the potential use of enterocin AS-48 to control E. coli O157:H7 in combination with other hurdles.
authors.affiliation: Departamento de Microbiología, Universidad de Granada, Granada, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Ananou
authors.forename: A
authors.initials: A
authors.lastname: Gálvez
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Bueno
authors.forename: M
authors.initials: M
authors.lastname: Maqueda
authors.forename: E
authors.initials: E
authors.lastname: Valdivia
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001628:Beverages; D001952:Bridged-Ring Compounds / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D019453:Escherichia coli O157 / Q000187:drug effects*; D005506:Food Contamination; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D027845:Malus
chemical_list: D000900:Anti-Bacterial Agents; D001952:Bridged-Ring Compounds; D002614:Chelating Agents; C012306:enterocin