Sugar-sensitive thin films composed of concanavalin A and sugar-bearing polymers.
Layered thin films composed of concanavalin A (Con A) and sugar-bearing polymers were prepared by a layer-by-layer deposition of Con A and the polymer on a solid surface. The sugar-induced disintegration was studied. Con A-polymer layered films could be successfully prepared using a maltose-bearing polymer (PV-MA), while melibiose- and glucose-bearing polymers (PV-MEA and PV-G) did not afford a layered film, due to a weak affinity of PV-MEA and PV-G to Con A. The Con A/PV-MA layered film was stable in pH 7 and 8 solutions, while in a pH 6 medium the film was slightly unstable. The Con A/PV-MA film was disintegrated upon the addition of sugars in solution owing to a preferential binding of the sugars to the binding site of Con A in the film. The disintegration rate was dependent on the type of sugar and its concentration. The Con A/PV-MA film was disintegrated rapidly upon the addition of methyl alpha-D-mannopyranoside, while the rate was slower upon the addition of the same concentration of D-mannose, D-glucose and methyl alpha-D-glucopyranoside. The present system may be useful for constructing sensitive devices that can release a drug or other functional molecules in response to sugars.
Sato
Katsuhiko
K
Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai, Japan.
Kodama
Daisuke
D
Anzai
Jun-ichi
J
D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry*; D003208:Concanavalin A / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D008320:Maltose / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D008553:Melibiose / Q000737:chemistry; D008757:Methylglucosides / Q000737:chemistry; D008766:Methylmannosides / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D002241:Carbohydrates; D008757:Methylglucosides; D008766:Methylmannosides; D011145:Polyvinyls; D003208:Concanavalin A; C008466:methylmannoside; C027020:methylglucoside; D008320:Maltose; D008553:Melibiose; D005947:Glucose; D008358:Mannose; D005690:Galactose