Expression profiling of herpesvirus and vaccinia virus proteins using a high-throughput baculovirus screening system.
We have developed a high-throughput system for generating baculoviruses and testing the expression, solubility, and affinity column purification of encoded proteins. We have used this system to generate baculoviruses for and analyze the expression of 337 proteins from three different herpesviruses (HSV-1, EBV, and CMV) and vaccinia virus. Subsets of these proteins were also tested for expression and solubility in E. coli. Comparisons of the results in the two systems are presented for each virus.
Gao
Mian
M
Affinium Pharmaceuticals, Toronto, Canada.
Brufatto
Nicole
N
Chen
Tricia
T
Murley
Laura Lea
LL
Thalakada
Rosanne
R
Domagala
Megan
M
Beattie
Bryan
B
Mamelak
Daniel
D
Athanasopoulos
Vicki
V
Johnson
David
D
McFadden
Grant
G
Burks
Christian
C
Frappier
Lori
L
D000818:Animals; D016367:Baculoviridae / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006564:Herpesviridae / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000378:metabolism; D007313:Insecta; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry; D014616:Vaccinia virus / Q000378:metabolism*
D020543:Proteome; D011994:Recombinant Proteins