title: Evaluation of new broth media for microdilution antibiotic susceptibility testing of Lactobacilli, Pediococci, Lactococci, and Bifidobacteria.
abstract: Nine pure or mixed broth media were evaluated for their suitabilities to determine MICs in a microdilution test of 19 antibacterial agents for lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Lactococcus, and Bifidobacterium. A mixed formulation of Iso-Sensitest broth (90%) and deMan-Rogosa-Sharpe broth (10%) with or without supplementation with L-cysteine, referred to as the LAB susceptibility test medium, provided the most optimal medium basis in terms of growth support of nonenterococcal LAB and correct indication of MICs of international control strains.
authors.affiliation: Robert Koch Institute, Wernigerode Branch, Burgstrasse 37, D-38855 Wernigerode, Germany. i.klare@rki.de
authors.forename: Ingo
authors.initials: I
authors.lastname: Klare
authors.forename: Carola
authors.initials: C
authors.lastname: Konstabel
authors.forename: Sibylle
authors.initials: S
authors.lastname: Müller-Bertling
authors.forename: Rolf
authors.initials: R
authors.lastname: Reissbrodt
authors.forename: Geert
authors.initials: G
authors.lastname: Huys
authors.forename: Marc
authors.initials: M
authors.lastname: Vancanneyt
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
authors.forename: Herman
authors.initials: H
authors.lastname: Goossens
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Witte
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001644:Bifidobacterium / Q000187:drug effects*; D003470:Culture Media; D016983:Enterococcus / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D007778:Lactobacillus / Q000187:drug effects*; D017045:Lactococcus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010376:Pediococcus / Q000187:drug effects*; D013210:Staphylococcus / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media