title: [Remodel retinal neovascularization by overexpression of HESR-1 in vitro].
abstract: OBJECTIVE
  To remodel retinal neovascularization by overexpression of HESR-1 in vitro.
  
  
  METHODS
  Human retinal capillary endothelial cell (HRCEC) was isolated, then HRCECs were transfected with pcDNA3.1+HESR-1. The changes of expression of KDR and occluding in HRCECs were analyzed using ELISA assay, the regulation of occluding in HRCECs by HESR-1 was evaluated by western blot.
  
  
  RESULTS
  Over-expression of HESR-1 down-regulated KDR expression, but occludin expression was up-regulated in HRECEs, the result also showed that the vessels permeability was reduced by HESR-1.
  
  
  CONCLUSIONS
  Overexpression of HESR-1 in HRCECs can inhibit the neovascularization and decrease the permeability of new vessels. It may be a promising gene therapy for intraocular neovascularization.
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. sbtang@gzsums.edu.cn
authors.forename: Shi-bo
authors.initials: SB
authors.lastname: Tang
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Ge
authors.initials: G
authors.lastname: Zhang
authors.forename: Jian-hong
authors.initials: JH
authors.lastname: Chen
authors.forename: Bao-jin
authors.initials: BJ
authors.lastname: Li
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D004730:Endothelium, Vascular / Q000378:metabolism; D015870:Gene Expression; D015316:Genetic Therapy; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D009389:Neovascularization, Pathologic; D062793:Occludin; D015861:Retinal Neovascularization / Q000235:genetics*; D012171:Retinal Vessels / Q000378:metabolism; D014162:Transfection; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018797:Cell Cycle Proteins; D008565:Membrane Proteins; D062793:Occludin; D040301:Vascular Endothelial Growth Factor Receptor-2; C485631:HEY1 protein, human; C568985:OCLN protein, human