title: NANOG changes in mouse kidneys with age.
abstract: NANOG is essential for mouse and human embryonic stem cell (ESC) pluripotency and selfrenewal. It is also expressed in several adult murine tissues as shown by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. However, human NANOG transcripts have been isolated from adult bone marrow (EST; GenBank accession no. BF893620). Here, we study the NANOG gene expression profile in isolated mouse renal papillary cells by Northern blot and RT-PCR. The whole RNA of mouse renal cells was obtained from fresh renal tissues, renal tissues infused by phosphate-buffered saline (PBS), and isolated renal papillary cells of mouse, respectively, as well as the renal papillary tissue from 18.5 days postcoitum (d.p.c.; fetal), 1-2-week-old (young), 1-8-month-old (adult), and 24-month-old (aging) mice. Our analysis shows that a very low expression level was detected in mouse renal tissues, and the renal papillary cells express more than other tissues as determined with Northern blot and RT-PCR. These data suggest that the kidney has its own cells expressing NANOG, and loss of NANOG expression occurs in an age-dependent manner in the kidney, either due to developmental factors or aging, particularly in renal papillary tissue.
authors.affiliation: Department of Nephrology, Kidney Center and Key Lab of PLA, Chinese General Hospital of PLA, Beijing, PR China.
authors.forename: Quan-Jian
authors.initials: QJ
authors.lastname: Yan
authors.forename: Xiang-Mei
authors.initials: XM
authors.lastname: Chen
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Zhang
authors.forename: Yuansheng
authors.initials: Y
authors.lastname: Xie
authors.forename: Suo-Zhu
authors.initials: SZ
authors.lastname: Shi
authors.forename: Bo
authors.initials: B
authors.lastname: Fu
authors.forename: Quan
authors.initials: Q
authors.lastname: Hong
authors.forename: Guo-Shuang
authors.initials: GS
authors.lastname: Xu
authors.forename: Xue-Guang
authors.initials: XG
authors.lastname: Zhang
authors.forename: Han-Yu
authors.initials: HY
authors.lastname: Zhu
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Yun-Hui
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics*; D007668:Kidney / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D000071317:Nanog Homeobox Protein
chemical_list: D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse