title: Brain-derived neurotrophic factor/glial cell line-derived neurotrophic factor survival effects on auditory neurons are not limited by dexamethasone.
abstract: Cochlear implant performance depends on the number of surviving excitable auditory neurons and prevention of degradation of nerve-electrode interaction caused by adverse tissue reactions. Glucocorticoids and neurotrophic factors are promising options for a possible therapeutic intervention. Neurons dissociated from the spiral ganglion of rats (3-5 days old) were cultivated with addition of brain-derived neurotrophic factor, glial cell line-derived neurotrophic factor and the corticosteroid dexamethasone in various concentrations (25, 50, 100 ng/ml) and in combination with each other (100 ng/ml). The results suggest that a combination of brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor does not enhance spiral ganglion cell survival significantly when compared with single brain-derived neurotrophic factor treatment (100 ng/ml). In addition, dexamethasone application did not interfere with the survival-promoting effects of brain-derived neurotrophic factor or glial cell line-derived neurotrophic factor.
authors.affiliation: Department of Otolaryngology, Medical University of Hannover, Hannover, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Wefstaedt
authors.forename: Verena
authors.initials: V
authors.lastname: Scheper
authors.forename: Thomas
authors.initials: T
authors.lastname: Lenarz
authors.forename: Timo
authors.initials: T
authors.lastname: Stöver
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001303:Auditory Cortex / Q000187:drug effects* / Q000502:physiology; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000494:pharmacology*; D007150:Immunohistochemistry; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013136:Spiral Ganglion / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D019208:Brain-Derived Neurotrophic Factor; D051100:Glial Cell Line-Derived Neurotrophic Factor; D003907:Dexamethasone