title: Spoligotype analysis of Mycobacterium bovis isolates from Northern México.
abstract: Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease is spread through this geographical region, particularly in countries that border those that are almost free of Mycobacterium bovis. In this work, we have analyzed the spacer oligonucleotide typing (spoligotype) patterns of M. bovis isolates from cattle at Ciudad Juárez, a Mexican city close to El Paso, Texas. Fifty-eight M. bovis isolates collected from a herd in Northern Mexico were studied by spoligotyping. Nine spoligotype patterns were observed in total. Two were predominant (SB0121 and SB0140) and accounted for 50% and 14% of the isolates, respectively. Six patterns were found to be already described in an international M. bovis spoligotype database, while the other three (SB0985, SB0986, and SB0987) were novel. Interestingly, none of the isolates corresponded to any other Mexican pattern previously reported. This is the first spoligotype analysis of M. bovis strains from a border city between Mexico and the United States. The necessity for further studies to formulate a better identification of M. bovis strains within, and its dissemination between, the two countries is discussed.
authors.affiliation: Department of Immunology, Escuela Nacional de Ciencias Biologicas, IPN. Prolongacion Carpio y Plan de Ayala s/n, Colonia Santo Tomas, Mexico, DF 11340, Mexico.
authors.forename: Laura
authors.initials: L
authors.lastname: Cobos-Marín
authors.forename: Jesus
authors.initials: J
authors.lastname: Montes-Vargas
authors.forename: Martín
authors.initials: M
authors.lastname: Zumarraga
authors.forename: Angel
authors.initials: A
authors.lastname: Cataldi
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Romano
authors.forename: Iris
authors.initials: I
authors.lastname: Estrada-Garcia
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Gonzalez-y-Merchand
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D002417:Cattle; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005838:Genotype; D008800:Mexico; D017720:Molecular Epidemiology; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014380:Tuberculosis, Bovine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial