title: Variability of quantitative polymerase chain reaction detection of the bcl-2-IgH translocation in an international multicenter study.
abstract: The t(14;18)(q32;q21) chromosomal translocation is closely associated with follicular lymphoma. Polymerase chain reaction (PCR) analysis has high sensitivity and is used to assess responses to therapy. Quantification of translocation-bearing cells is a possible advantage of real-time PCR over conventional PCR. A collaborative study comparing results from 12 international laboratories is reported.
authors.forename: Angela J
authors.initials: AJ
authors.lastname: Darby
authors.forename: Stuart
authors.initials: S
authors.lastname: Lanham
authors.forename: Pierre
authors.initials: P
authors.lastname: Soubeyran
authors.forename: Peter W M
authors.initials: PW
authors.lastname: Johnson
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics* / Q000648:ultrastructure; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics* / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D005188:False Negative Reactions; D005189:False Positive Reactions; D005803:Genes, Immunoglobulin; D019254:Genes, bcl-2; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007753:Laboratories / Q000706:statistics & numerical data; D008224:Lymphoma, Follicular / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014178:Translocation, Genetic
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains