A tuber lectin from Arisaema helleborifolium Schott with anti-insect activity against melon fruit fly, Bactrocera cucurbitae (Coquillett) and anti-cancer effect on human cancer cell lines.
An anti-insect and anti-cancer lectin has been isolated from Arisaema helleborifolium Schott by affinity chromatography using asialofetuin-linked amino activated silica beads. The bound A. helleborifolium lectin (AHL) was eluted with 100mM glycine-HCl buffer, pH 2.5. It gave a single band on SDS-PAGE, pH 8.3, and PAGE, pH 4.5. However, multiple bands were obtained in PAGE at pH 8.3 and isoelectric focusing. The lectin was a homotetramer having subunit molecular mass 13.4kDa while its native molecular mass was 52kDa. It was a glycoprotein with 3.40% carbohydrate and was stable up to 60 degrees C for 30min. It showed anti-insect activity towards second instar larvae of Bactrocera cucurbitae (Coquillett) with LC(50) value of 16.4microg/ml. Larvae fed on artificial diet containing sub-lethal dose of AHL showed a significant decrease in acid phosphatase and alkaline phosphatase activity while esterase activity markedly increased as compared to larvae fed on diet without lectin. AHL was also found to inhibit in vitro proliferation of some well established human cancer cell lines viz HOP-62 (95%), HCT-15 (92%), HEP-2 (66%), HT-29 (68%), PC-3 (39.4%), and A-549 (20.7%).
Kaur
Manpreet
M
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar 143 005, India.
Singh
Kuljinder
K
Rup
Pushpinder J
PJ
Saxena
A K
AK
Khan
Rizwan H
RH
Ashraf
Mohd Tashfeen
MT
Kamboj
Sukhdev Singh
SS
Singh
Jatinder
J
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity; D004950:Esterases / Q000378:metabolism; D006801:Humans; D007306:Insecticides / Q000302:isolation & purification / Q000494:pharmacology*; D007814:Larva; D037121:Plant Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D033621:Tephritidae / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development
D000970:Antineoplastic Agents; D007306:Insecticides; D037121:Plant Lectins; D004950:Esterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase