title: Functional sulfurtransferase is associated with mitochondrial complex I from Yarrowia lipolytica, but is not required for assembly of its iron-sulfur clusters.
abstract: Here, we report that in the obligate aerobic yeast Yarrowia lipolytica, a protein exhibiting rhodanese (thiosulfate:cyanide sulfurtransferase) activity is associated with proton pumping NADH:ubiquinone oxidoreductase (complex I). Complex I is a key enzyme of the mitochondrial respiratory chain that contains eight iron-sulfur clusters. From a rhodanese deletion strain, we purified functional complex I that lacked the additional protein but was fully assembled and displayed no functional defects or changes in EPR signature. In contrast to previous suggestions, this indicated that the sulfurtransferase associated with Y. lipolytica complex I is not required for assembly of its iron-sulfur clusters.
authors.affiliation: Ulrich Brandt, Universität Frankfurt, Fachbereich Medizin, Zentrum der Biologischen Chemie, Molekulare Bioenergetik, Theodor-Stern-Kai 7, Haus 26, 60590 Frankfurt am Main, Germany.
authors.forename: Albina
authors.initials: A
authors.lastname: Abdrakhmanova
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Dobrynin
authors.forename: Klaus
authors.initials: K
authors.lastname: Zwicker
authors.forename: Stefan
authors.initials: S
authors.lastname: Kerscher
authors.forename: Ulrich
authors.initials: U
authors.lastname: Brandt
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D002384:Catalysis; D004578:Electron Spin Resonance Spectroscopy; D042967:Electron Transport Complex I / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D017353:Gene Deletion; D005800:Genes, Fungal; D007425:Intracellular Membranes / Q000201:enzymology; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000235:genetics; D021122:Protein Subunits / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013466:Sulfurtransferases / Q000378:metabolism*; D013884:Thiosulfate Sulfurtransferase / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology; D025062:Yarrowia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D007506:Iron-Sulfur Proteins; D021122:Protein Subunits; D009247:NADH, NADPH Oxidoreductases; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase; D014357:Trypsin; D042967:Electron Transport Complex I