title: Differential effects of clathrin and actin inhibitors on internalization of Escherichia coli and Salmonella choleraesuis in porcine jejunal Peyer's patches.
abstract: Peyer's patches constitute both an inductive immune site and an enteropathogen invasion route. Peyer's patch mucosae from porcine jejunum were mounted in Ussing chambers, and either Salmonella choleraesuis vaccine strain SC-54 or non-pathogenic rodent and porcine Escherichia coli strains contacted the Peyer's patch mucosa for 90 min. Internalized bacteria were quantified by a gentamicin resistance assay. Monodansylcadaverine (300 microM, luminal addition), an inhibitor of clathrin-mediated endocytosis, significantly inhibited internalization of both E. coli strains relative to tissues untreated with the inhibitor; internalization of SC-54 was unaffected. The actin-disrupting agent cytochalasin D (10 microM, luminal addition), inhibited internalization of pig-adapted E. coli but not that of rodent-adapted E. coli or SC-54. Internalization of SC-54 and non-pathogenic E. coli in Peyer's patches appears to occur through different cellular routes.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Minnesota, 1988 Fitch Avenue, St. Paul, MN 55108-6010, USA.
authors.forename: Benedict T
authors.initials: BT
authors.lastname: Green
authors.forename: David R
authors.initials: DR
authors.lastname: Brown
mesh_terms: D000199:Actins / Q000037:antagonists & inhibitors*; D000818:Animals; D002103:Cadaverine / Q000031:analogs & derivatives / Q000494:pharmacology; D002966:Clathrin / Q000037:antagonists & inhibitors*; D015638:Cytochalasin D / Q000494:pharmacology; D004553:Electric Conductivity; D004705:Endocytosis / Q000187:drug effects; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000472:pathogenicity; D005260:Female; D007583:Jejunum; D008297:Male; D010581:Peyer's Patches / Q000187:drug effects / Q000382:microbiology*; D012475:Salmonella / Q000187:drug effects* / Q000472:pathogenicity; D013552:Swine
chemical_list: D000199:Actins; D002966:Clathrin; D015638:Cytochalasin D; C008542:monodansylcadaverine; D002103:Cadaverine