title: Collagen XXVIII, a novel von Willebrand factor A domain-containing protein with many imperfections in the collagenous domain.
abstract: Here we describe a novel collagen belonging to the class of von Willebrand factor A (VWA) domain-containing proteins. This novel protein was identified by screening the EST data base and was subsequently recombinantly expressed and characterized as an authentic tissue component. The COL28A1 gene on human chromosome 7p21.3 and on mouse chromosome 6A1 encodes a novel protein that structurally resembles the beaded filament-forming collagens. The collagenous domain contains several very short interruptions arranged in a repeat pattern. As shown for other novel minor collagens, the expression of collagen XXVIII protein in mouse is very restricted. In addition to small amounts in skin and calvaria, the major signals were in dorsal root ganglia and peripheral nerves. By immunoelectron microscopy, collagen XXVIII was detected in the sciatic nerve, at the basement membrane of certain Schwann cells surrounding the nerve fibers. Even though the protein is present in the adult sciatic nerve, collagen XXVIII mRNA was only detected in sciatic nerve of newborn mice, indicating that the protein persists for an extended period after synthesis.
authors.affiliation: Center for Biochemistry, Center for Molecular Medicine, and Department of Dermatology, Medical Faculty, University of Cologne, D-50931 Cologne, Germany.
authors.forename: Guido
authors.initials: G
authors.lastname: Veit
authors.forename: Birgit
authors.initials: B
authors.lastname: Kobbe
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Keene
authors.forename: Mats
authors.initials: M
authors.lastname: Paulsson
authors.forename: Manuel
authors.initials: M
authors.lastname: Koch
authors.forename: Raimund
authors.initials: R
authors.lastname: Wagener
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D007611:Aprotinin / Q000737:chemistry; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D003094:Collagen / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D020224:Expressed Sequence Tags; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007477:Ions; D051379:Mice; D008810:Mice, Inbred C57BL; D016253:Microscopy, Immunoelectron; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D016366:Open Reading Frames; D010802:Phylogeny; D010957:Plasmids / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000378:metabolism; D012584:Sciatic Nerve / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014841:von Willebrand Factor / Q000737:chemistry*
chemical_list: C509350:COL28A1 protein, human; D018076:DNA, Complementary; D007477:Ions; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014841:von Willebrand Factor; D003094:Collagen; D007611:Aprotinin