Simultaneous quantification of six major phenolic acids in the roots of Salvia miltiorrhiza and four related traditional Chinese medicinal preparations by HPLC-DAD method.
A high-performance liquid chromatographic method was applied to the determination of danshensu, protocatechuic aldehyde, rosmarinic acid, lithospermic acid, salvianolic acid B and salvianolic acid A in the roots of Salvia miltiorrhiza and four related traditional Chinese medicinal preparations. The six phenolic acids were simultaneously analyzed with a Zorbax Extend C18 column by gradient elution using 0.026% (v/v) phosphoric acid and acetonitrile as the mobile phase. The flow rate was 1 ml min(-1), and detection wavelength was set at 288 nm. The recovery of the method was in the range of 95.1-104.8%, and all the compounds showed good linearity (r > 0.9997) in a relatively wide concentration range. This assay was successfully applied to the determination of six major phenolic acids in 32 samples. The results indicated that the developed HPLC assay could be readily utilized as a quality control method for S. miltiorrhiza and its related traditional Chinese medicinal preparations.
Liu
Ai-Hua
AH
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100083, PR China.
Li
Lie
L
Xu
Man
M
Lin
Yan-Hua
YH
Guo
Hong-Zhu
HZ
Guo
De-An
DA
D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D062385:Hydroxybenzoates / Q000737:chemistry; D008516:Medicine, Chinese Traditional; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000378:metabolism*; D013678:Technology, Pharmaceutical / Q000379:methods
D062385:Hydroxybenzoates; D010936:Plant Extracts; C017616:phenolic acid; D000432:Methanol