Quantitation of Bacillus clausii in biological samples by real-time polymerase chain reaction.
A real-time PCR assay targeting the highly specific erm34 sequence of Bacillus clausii DNA was developed and optimized. The quantitative assay showed a sensitivity level of 10(2) CFU/microl of sample. The method may represent a useful tool for monitoring the role of B. clausii as probiotic in vivo.
Perotti
Mario
M
Università Vita-Salute San Raffaele and Laboratorio di Microbiologia e Virologia, Diagnostica e Ricerca San Raffaele, Milan, Italy. mario.perotti@hsr.it
Mancini
Nicasio
N
Cavallero
Annalisa
A
Carletti
Silvia
S
Canducci
Filippo
F
Burioni
Roberto
R
Clementi
Massimo
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001398:Aztreonam / Q000494:pharmacology; D001407:Bacillus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics; D002701:Chloramphenicol / Q000494:pharmacology; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008780:Methyltransferases / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019936:Probiotics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D002701:Chloramphenicol; D008780:Methyltransferases; D001398:Aztreonam