title: Mapping the distribution of 3-hydroxy oxylipins in the ascomycetous yeast Saturnispora saitoi.
abstract: The distribution of 3-hydroxy oxylipins in Saturnispora saitoi was mapped using immunofluorescence microscopy. Fluorescence was observed on aggregating ascospores, indicating the presence of 3-hydroxy oxylipins on the surface or between ascospores. The oxylipin was identified as 3-hydroxy 9:1 using gas chromatography mass spectrometry. Furthermore, ultrastructural studies using scanning and transmission electron microscopy on ascospores revealed a clear equatorial ledge surrounding oval-shaped ascospores.
authors.affiliation: Department of Microbial, Biochemical and Food Biotechnology, UNESCO MIRCEN: Industrial Biotechnology, University of the Free State, P.O. Box 339, Bloemfontein, South Africa.
authors.forename: Andries S
authors.initials: AS
authors.lastname: Bareetseng
authors.forename: Johan L F
authors.initials: JL
authors.lastname: Kock
authors.forename: Carolina H
authors.initials: CH
authors.lastname: Pohl
authors.forename: Elma E
authors.initials: EE
authors.lastname: Pretorius
authors.forename: Catharina J
authors.initials: CJ
authors.lastname: Strauss
authors.forename: Piet J
authors.initials: PJ
authors.lastname: Botes
authors.forename: Pieter W J
authors.initials: PW
authors.lastname: Van Wyk
authors.forename: Santosh
authors.initials: S
authors.lastname: Nigam
mesh_terms: D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D004718:Saccharomycetales / Q000737:chemistry* / Q000648:ultrastructure; D013172:Spores, Fungal / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D005227:Fatty Acids