[Effects of prazosin on the proliferation and apoptosis of K562 leukemia cells].
OBJECTIVE
To investigate the effects of prazosin on the proliferation and apoptosis of human leukemia cell line (K562).


METHODS
The effects of prazosin on the proliferation of K562 cells were evaluated by liquid culture assay, MTT assay and colony formation assay. Prazosin induced K562 cells toward apoptosis and this was verified by cellular morphology, Hoechst33258 fluorescence staining and flow cytometry respectively.


RESULTS
The proliferation of K562 leukemia cells was inhibited by prazosin in a dose-dependent manner. With prazosin (5 x 10(-6) mol/L), the apoptosis cells could be observed morphologically, or by Hoechst 33258 fluorescence staining and flow cytometry.


CONCLUSIONS
Prazosin can inhibit the proliferation of K562 cells and induce the apoptosis of K562 cells.
He
Jun
J
Laboratory of Medical Genetics of Changsha Mother and Child Health Care Hospital, Changsha, China. hq20002002@yahoo.com.cn
He
Qun
Q
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D011224:Prazosin / Q000494:pharmacology*
D000317:Adrenergic alpha-Antagonists; D000970:Antineoplastic Agents; D011224:Prazosin