title: Isolation of equine herpesvirus-2 from the lung of an aborted fetus.
abstract: This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause-effect relationship was established between the EHV-2 isolate and the abortion.
authors.affiliation: Department of Virology, Faculty of Veterinary Sciences, National University of La Plata, Buenos Aires Province, Argentina.
authors.forename: Cecilia Mónica
authors.initials: CM
authors.lastname: Galosi
authors.forename: Viviana Cid
authors.initials: VC
authors.lastname: de la Paz
authors.forename: Leandro Carlos
authors.initials: LC
authors.lastname: Fernández
authors.forename: Javier Pablo
authors.initials: JP
authors.lastname: Martinez
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Craig
authors.forename: María
authors.initials: M
authors.lastname: Barrandeguy
authors.forename: María Elisa
authors.initials: ME
authors.lastname: Etcheverrrigaray
mesh_terms: D037881:Aborted Fetus / Q000821:virology*; D000818:Animals; D016172:DNA Fingerprinting / Q000662:veterinary; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D008168:Lung / Q000196:embryology / Q000821:virology*; D009500:Neutralization Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D018144:Rhadinovirus / Q000235:genetics / Q000302:isolation & purification*; D014412:Tumor Virus Infections / Q000662:veterinary* / Q000821:virology
chemical_list: D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI