title: [The effect of mutation dominant spotting-Yurlovo (KitW-Y) on spermatogenesis, early embryogenesis, and fertility of C57BL/6JY mice].
abstract: The effect of mutation KitW-Y found in C57BL/6 mice on fertility, spermatogenesis, and early embryogenesis of mice have been studied. If heterozygotes KitW-Y/+ are crossed with wild-type mice, fertility decreases by 20%. Homozygotes Kitw-Y/KitW-Y and compounds KitW-Y/KitSsm are nonviable. The study of spermatogenesis in KitW-Y/+ mice has demonstrated a negative effect of this mutation on spermatocytes. Histological examination of the testes of mutant males has shown local empty spaces in seminal ducts. Electron microscopic examination of synaptonemal complexes have demonstrated desynapsis disturbance in some nuclei at the diplotene stage of meiotic prophase I. However, these disturbances do not cause a decrease in the number of fertilized oocytes/ova. The decrease in fertility is accounted for disturbances of early embryogenesis. In vivo and in vitro analyses of early embryogenesis have demonstrated that cleavage divisions are asynchronous in KitW-Y/+ heterozygous embryos. Some of these embryos die before implantation, and others cleave more rapidly than wildtype embryos, which give them selective advantage during the postimplantation period of embryogenesis. The pattern of KitW-Y expression during spermatogenesis and embryogenesis mimics potential human pathology, which makes these mutants an interesting and valuable object for genetics and developmental biology.
authors.forename: O L
authors.initials: OL
authors.lastname: Kolomiets
authors.forename: L F
authors.initials: LF
authors.lastname: Kurilo
authors.forename: A M
authors.initials: AM
authors.lastname: Malashenko
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Sakharova
authors.forename: T A
authors.initials: TA
authors.lastname: Chebotareva
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D047108:Embryonic Development / Q000235:genetics*; D005298:Fertility / Q000235:genetics*; D006579:Heterozygote; D008297:Male; D051379:Mice; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics; D012671:Seminiferous Tubules / Q000166:cytology; D013091:Spermatogenesis / Q000235:genetics*
chemical_list: D019009:Proto-Oncogene Proteins c-kit