title: Yeast community survey in the Tagus estuary.
abstract: The yeast community in the waters of the Tagus estuary, Portugal, was followed for over a year in order to assess its dynamics. Yeast occurrence and incidence were measured and this information was related to relevant environmental data. Yeast occurrence did not seem to depend upon tides, but river discharge had a dramatic impact both on the density and diversity of the community. The occurrence of some yeasts was partially correlated with faecal pollution indicators. Yeast isolates were characterized by microsatellite primed PCR (MSP-PCR) fingerprinting and rRNA gene sequencing. The principal species found were Candida catenulata, C. intermedia, C. parapsilosis, Clavispora lusitaniae, Debaryomyces hansenii, Pichia guilliermondii, Rhodotorula mucilaginosa and Rhodosporidium diobovatum. The incidence of these species was evaluated against the environmental context of the samples and the current knowledge about the substrates from which they are usually isolated.
authors.affiliation: CREM - Centro de Recursos Microbiológicos, Fac. de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, Caparica, Portugal.
authors.forename: João M G C F
authors.initials: JM
authors.lastname: de Almeida
mesh_terms: D002965:Classification; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis; D004784:Environmental Monitoring; D016133:Polymerase Chain Reaction; D011157:Population Dynamics; D011174:Portugal; D012335:RNA, Ribosomal / Q000032:analysis; D014871:Water Microbiology; D015003:Yeasts
chemical_list: D004271:DNA, Fungal; D012335:RNA, Ribosomal