title: In situ fabrication of three-dimensional chemical patterns in fused silica separation capillaries with polymerized phospholipids.
abstract: We report a new molecular approach for in situ generation of micron scale, chemically and biochemically functionalized patterns inside three-dimensional, completely enclosed fluidic channels. The formation of chemical patterns is based upon a combination of lipid bilayer self-assembly and UV photopolymerization of photoreactive, cross-linkable phospholipids. Using this approach, we have functionalized capillaries of varying inner diameters with a range of chemistries useful for protein and peptide immobilization. Here, we demonstrate the ability to produce small molecule and protein-based chemical patterns.
authors.affiliation: Department of Chemistry, University of Arizona, Tucson, 85721, USA.
authors.forename: Eric E
authors.initials: EE
authors.lastname: Ross
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Mansfield
authors.forename: Yiding
authors.initials: Y
authors.lastname: Huang
authors.forename: Craig A
authors.initials: CA
authors.lastname: Aspinwall
mesh_terms: D008051:Lipid Bilayers / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D010778:Photochemotherapy; D012822:Silicon Dioxide / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010743:Phospholipids; C077390:1,2-bis(10(2',4'-hexadienoyloxy)decanoyl)-sn-glycero-3-phosphocholine; D012822:Silicon Dioxide