Cloning and characterization of the CDZFP gene which encodes a putative zinc finger protein.
We report here the cloning and characterization of a novel human cytoplasm-distribution zinc finger protein (CDZFP) gene, isolated from human ovary cDNA library, and mapped to 4p12 by searching the UCSC genomic database. The CDZFP cDNA is 1793 base pairs in length and contains an open reading frame (ORF) encoding 236 amino acids. The CDZFP gene consists of 7 exons and encodes a putative zinc finger protein with a transmembrane region and two zinc finger motifs. Subcellular localization demonstrated that CDZFP protein was located in the cytoplasm when overexpressed in Hela cells and northern blot analysis revealed that CDZFP was ubiquitously expressed in 16 human tissues.
Tang
Wenwen
W
State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, China.
Yuan
Jian
J
Chen
Xinya
X
Shan
Yuxi
Y
Luo
Kuntian
K
Guo
Zekun
Z
Zhang
Yue
Y
Wan
Bo
B
Yu
Long
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009928:Organ Specificity; D017434:Protein Structure, Tertiary; D016335:Zinc Fingers
D002352:Carrier Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; C508936:NFXL1 protein, human