NtPDR3, an iron-deficiency inducible ABC transporter in Nicotiana tabacum.
In plants, the ABC transporter PDR (pleiotropic drug resistance) subfamily is composed of approximately 15 genes, few of which have been analyzed. We have identified NtPDR3, a Nicotiana tabacum PDR gene belonging to a cluster for which no functional data was previously available. NtPDR3 was found to be induced in suspension cells treated with methyl jasmonate, salicylic acid, 1-naphthalene acetic acid, or cembrene, a macrocyclic diterpene. In agreement with the identification of a putative iron deficiency element in the NtPDR3 transcription promoter region, we found that iron deficiency in the culture medium induced NtPDR3 expression, thus suggesting a new function of the PDR transporter family.
Ducos
Eric
E
Unité de Biochimie Physiologique, Institut des Sciences de la Vie, Université Catholique de Louvain, Croix du Sud, 5, B-1348 Louvain-la-Neuve, Belgium.
Fraysse
Staffan
S
Boutry
Marc
M
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000085:Acetates / Q000494:pharmacology; D001483:Base Sequence; D001692:Biological Transport; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003470:Culture Media / Q000032:analysis; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary; D004224:Diterpenes / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005822:Genetic Vectors; D000090463:Iron Deficiencies; D008969:Molecular Sequence Data; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D054883:Oxylipins; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D020156:Salicylic Acid / Q000494:pharmacology; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic
D018528:ATP-Binding Cassette Transporters; D000085:Acetates; D002614:Chelating Agents; D003470:Culture Media; D003517:Cyclopentanes; D018076:DNA, Complementary; D004224:Diterpenes; D009280:Naphthaleneacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C108204:cembrene A; C034182:1-naphthaleneacetic acid; D004533:Egtazic Acid; C072239:methyl jasmonate; D020156:Salicylic Acid