Cooperativity between 11beta-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase is based on a common pyridine nucleotide pool in the lumen of the endoplasmic reticulum.
11Beta-hydroxysteroid dehydrogenase type 1 (11betaHSD1) is a NADP(H)-dependent oxidoreductase of the ER lumen, which may have an important role in the pathogenesis of metabolic syndrome. Here, the functional coupling of 11beta-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase (H6PDH) was investigated in rat liver microsomal vesicles. The results demonstrate the existence of a separate intraluminal pyridine nucleotide pool in the hepatic endoplasmic reticulum and a close cooperation between 11betaHSD1 and H6PDH based on their co-localization and the mutual generation of cofactors for each other.
Czegle
Ibolya
I
The Endoplasmic Reticulum Research Group of the Hungarian Academy of Sciences and Semmelweis University, 1444 Budapest, Hungary.
Piccirella
Simona
S
Senesi
Silvia
S
Csala
Miklós
M
Mandl
József
J
Bánhegyi
Gábor
G
Fulceri
Rosella
R
Benedetti
Angelo
A
D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000032:analysis / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D011725:Pyridines / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D022161:Transport Vesicles / Q000201:enzymology / Q000378:metabolism
D011725:Pyridines; D002237:Carbohydrate Dehydrogenases; C020552:galactose-6-phosphate dehydrogenase; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; C023666:pyridine