Recovery of the olfactory receptor neurons in the African Tilapia mariae following exposure to low copper level.
Low levels of Cu(2+) are known to specifically cause olfactory neuron death in fish olfactory epithelium. This study investigated the morphological changes in the olfactory mucosa of the cichlid Tilapia mariae, after a 4-day exposure to different concentrations of Cu(2+) (20, 40 and 100 microg/l), and the regeneration time-frame, when fish exposed to 20 microg/l were returned to dechlorinated tap water. Light microscopy, combined with Fluoro Jade-B staining, permitted the observation of a dose-dependent damage which became less severe and more circumscribed to receptor cells when Cu(2+) concentration decreased. The regeneration process in the olfactory tissue was examined in fish after 0, 3, and 10 days of recovery in well water. Immunostaining with PCNA showed a massive mitotic activity in the basal region of the mucosa immediately after exposure was terminated. The mitotically produced elements were immature neurons since they expressed the neural growth-associated phosphoprotein GAP-43. After 3 days of recovery the nuclei had already completed their migration to the upper portion of the epithelium and mitotic activity was much less intensive. After 10 days the olfactory tissue did not present differences when compared to the control tissue. These results suggest that after 10 days the regeneration is completed and the integrity of the tissue restored.
Bettini
Simone
S
Department of Biology, University of Bologna, Italy.
Ciani
Franco
F
Franceschini
Valeria
V
D000818:Animals; D003300:Copper / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D019922:GAP-43 Protein / Q000032:analysis / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary; D009410:Nerve Degeneration / Q000188:drug therapy; D009416:Nerve Regeneration / Q000187:drug effects*; D009831:Olfactory Mucosa / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D018034:Olfactory Receptor Neurons / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D017210:Tilapia / Q000502:physiology*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D019922:GAP-43 Protein; D018809:Proliferating Cell Nuclear Antigen; D014874:Water Pollutants, Chemical; D003300:Copper