A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes.
Two sPPases (soluble inorganic pyrophosphatases, EC 3.6.1.1) have been isolated from the microalga Chlamydomonas reinhardtii. Both are monomeric proteins of organellar localization, the chloroplastic sPPase I [Cr (Ch. reinhardtii)-sPPase I, 30 kDa] is a major isoform and slightly larger protein than the mitochondrial sPPase II (Cr-sPPase II, 24 kDa). They are members of sPPase family I and are encoded by two different cDNAs, as demonstrated by peptide mass fingerprint analysis. Molecular phylogenetic analyses indicated that Cr-sPPase I is closely related to other eukaryotic sPPases, whereas Cr-sPPase II resembles its prokaryotic counterparts. Chloroplastic sPPase I may have replaced a cyanobacterial ancestor very early during plastid evolution. Cr-sPPase II orthologues are found in members of the green photosynthetic lineage, but not in animals or fungi. These two sPPases from photosynthetic eukaryotes are novel monomeric family I sPPases with different molecular phylogenies and cellular localizations.
Gómez-García
María R
MR
Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-Universidad de Sevilla, Centro de Investigaciones Científicas Isla de la Cartuja, 41092-Sevilla, Spain. mrgomez@stanford.edu
Losada
Manuel
M
Serrano
Aurelio
A
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology; D016825:Chlamydomonas reinhardtii / Q000201:enzymology* / Q000378:metabolism; D002736:Chloroplasts / Q000201:enzymology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D043564:Inorganic Pyrophosphatase / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010788:Photosynthesis; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D020033:Protein Isoforms; D021381:Protein Transport; D016415:Sequence Alignment
D018076:DNA, Complementary; D020033:Protein Isoforms; D043564:Inorganic Pyrophosphatase