Separation of normoblasts from whole blood by ultracentrifugation on arabino-galactan discontinuous gradients: A tool for prenatal diagnosis.
Normoblasts can be separated at high resolution from whole blood by ultracentrifugation on discontinuous gradients of arabino-galactane after the red blood cells are removed by sedimentation. With this method, isolation of fetal normoblasts from maternal blood could provide a tool for rapid prenatal diagnosis. The availability of this noninvasive technique could avoid the cost and the risks of miscarriage that are associated with the current invasive procedures.
Piomelli
Sergio
S
Columbia University, New York, New York 10027-6902, USA. sp23@columbia.edu
Hirsch
Daniel
D
Corash
Lawrence
L
D000755:Anemia, Sickle Cell / Q000097:blood / Q000175:diagnosis*; D001773:Blood Cells / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D005315:Fetal Diseases / Q000175:diagnosis*; D005331:Fetomaternal Transfusion / Q000097:blood*; D005685:Galactans; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D013789:Thalassemia / Q000097:blood / Q000175:diagnosis*; D014461:Ultracentrifugation / Q000379:methods*
D005685:Galactans; C005653:arabinogalactan