title: Retroposition of processed pseudogenes: the impact of RNA stability and translational control.
abstract: Human processed pseudogenes are copies of cellular RNAs reverse transcribed and inserted into the nuclear genome by the enzymatic machinery of L1 (LINE1) non-LTR retrotransposons. Although it is generally accepted that germline expression is crucial for the heritable retroposition of cellular mRNAs, little is known about the influences of RNA stability, mRNA quality control and compartmentalization of translation on the retroposition of processed pseudogenes. We found that frequently retroposed human mRNAs are derived from stable transcripts with translation-competent functional reading frames that are resistant to nonsense-mediated RNA decay. They are preferentially translated on free cytoplasmic ribosomes and encode soluble proteins. Our results indicate that interactions between mRNAs and L1 proteins seem to occur at free cytoplasmic ribosomes.
authors.affiliation: Genetic Information Research Institute, 1925 Landings Drive, Mountain View, CA 94043, USA.
authors.forename: Adam
authors.initials: A
authors.lastname: Pavlicek
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Gentles
authors.forename: Jan
authors.initials: J
authors.lastname: Paces
authors.forename: Václav
authors.initials: V
authors.lastname: Paces
authors.forename: Jerzy
authors.initials: J
authors.lastname: Jurka
mesh_terms: D000818:Animals; D015870:Gene Expression; D006801:Humans; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics*; D014176:Protein Biosynthesis; D011544:Pseudogenes / Q000235:genetics*; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018626:Retroelements / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D018626:Retroelements