Selection of peptides for lipopolysaccharide binding on to epoxy beads and selective detection of Gram-negative bacteria.
Lipopolysaccharide (LPS)-binding peptides were enriched by using epoxy beads as a novel support to immobilize LPS for a phage displayed peptide library screening. The sequence of Phe-Ala-Pro-Trp (FAPW) was the most significant consensus motif of 10 selected clones, and Pro-Phe (PF) was the key dipeptide for binding at the apex of the loop to form a characteristic structure of CXXPFXXXC. Moreover, AWLPWAK, one of the highly conserved heptamer peptides, could detect specifically Gram-negative bacteria via a whole cell binding test at 10(6) cells ml(-1).
Kim
Yun-Gon
YG
Program in Biochemical Engineering and Biotechnology, Seoul National University, Gwanak-Gu, Korea.
Lee
Chang-Soo
CS
Chung
Woo-Jae
WJ
Kim
Eun-Mi
EM
Shin
Dong-Sik
DS
Kim
June-Hyung
JH
Lee
Yoon-Sik
YS
Chung
Junho
J
Kim
Byung-Gee
BG
D015373:Bacterial Typing Techniques; D004852:Epoxy Compounds / Q000737:chemistry*; D006090:Gram-Negative Bacteria; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D008863:Microspheres; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012680:Sensitivity and Specificity
D004852:Epoxy Compounds; D008070:Lipopolysaccharides; D009842:Oligopeptides