Isolation and characterization of a 30 kD antifungal protein from seeds of Sorghum bicolor.
An antifungal protein of about 30,000 Da was isolated from seeds of Sorghum bicolor L. using chromatographic techniques, including gel filtration, ion exchange, and high-performance liquid chromatography in a reverse-phase column. This protein (termed 30 kD protein) showed a minimal inhibitory concentration of 36 microg/ml for Candida parapsilosis and C. tropicalis, and 18 microg/ml for C. albicans. The 30 kD protein inhibited adherence to the cover glass and formation of a germinative tube of C. albicans at concentrations over 300 and 150 microg/ml, respectively. Transmission electron microscopy of yeast forms of C. albicans after incubation with 18 mug/ml of the 30 kD protein for 24 h revealed marked ultrastructural changes in the fungus. No toxicity of the 30 kD protein to the culture of Hep2 cells at concentrations equal to or less than 1,000 microg/ml was observed.
Mincoff
Patricia Costa
PC
Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, Av. Colombo, 5790, 87020-900 Maringá, PR, Brazil.
Garcia Cortez
Diógenes Aparício
DA
Ueda-Nakamura
Tânia
T
Nakamura
Celso Vataru
CV
Dias Filho
Benedito Prado
BP
D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D002176:Candida albicans / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D041022:Candida tropicalis / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D002448:Cell Adhesion / Q000187:drug effects; D005898:Glass; D046529:Microscopy, Electron, Transmission; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D045868:Sorghum / Q000737:chemistry*
D000935:Antifungal Agents; D010940:Plant Proteins