title: Assembly of layer-by-layer films of heme proteins and single-walled carbon nanotubes: electrochemistry and electrocatalysis.
abstract: After being treated by mixed acids, single-walled carbon nanotubes (SWNTs) were shortened and had negatively charged groups on the surface. Positively charged hemoglobin or myoglobin at pH 5.0 was successfully assembled with SWNTs into layer-by-layer films on solid surfaces, designated as {SWNT/protein}n. While only those proteins in the first few bilayers closest to the electrode surface exhibited electroactivity, the {SWNT/protein}n films demonstrated a much higher fraction of electroactive proteins and better controllability in film construction compared with cast films of the proteins and carbon nanotubes. The proteins in the {SWNT/protein}n films retained their near-native structure at medium pH. The stable protein film electrode showed good electrocatalytic properties toward reduction of oxygen and hydrogen peroxide, demonstrating the potential application of the {SWNT/protein}n films as a new type of biosensor based on the direct electrochemistry of proteins without using mediators.
authors.affiliation: Department of Chemistry, Beijing Normal University, Beijing, 100875, People's Republic of China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Zhao
authors.forename: Hongyun
authors.initials: H
authors.lastname: Liu
authors.forename: Naifei
authors.initials: N
authors.lastname: Hu
mesh_terms: D000143:Acids; D002384:Catalysis; D004563:Electrochemistry; D006420:Hemeproteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011791:Quartz; D013057:Spectrum Analysis
chemical_list: D000143:Acids; D006420:Hemeproteins; D037742:Nanotubes, Carbon; D011791:Quartz