Human herpesvirus 6 (HHV-6) U94/REP protein inhibits betaherpesvirus replication.
Human herpesvirus 6 (HHV-6) is the only human herpesvirus encoding U94/rep, homologue to the parvovirus non-structural gene rep68/78. Results to date suggest that HHV-6 U94/rep might regulate viral gene expression and have a role in viral latency. To determine the effect of U94/REP upon viral replication, the protein was produced. The purified U94/REP retained the characteristic immunological features. It was internalized and localized in the nucleus of human cells, showing marked inhibitory activity on the replication of HHV-6 (both variants A and B). The effect of U94/REP was dose-dependent and sensitive to treatment with single-stranded but not double-stranded DNA. U94/REP inhibited the replication of other betaherpesviruses, HHV-7 and human cytomegalovirus, but had no effect on herpes simplex virus. These results confirm the action of U94/rep latency gene in the regulation of HHV-6 replication with implications for co-reactivations and latency of human betaherpesviruses.
Caselli
Elisabetta
E
Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Via Borsari 46, 44100 Ferrara, Italy.
Bracci
Arianna
A
Galvan
Monica
M
Boni
Michela
M
Rotola
Antonella
A
Bergamini
Carlo
C
Cermelli
Claudio
C
Dal Monte
Paola
P
Gompels
Ursula A
UA
Cassai
Enzo
E
Di Luca
Dario
D
D018141:Betaherpesvirinae / Q000502:physiology*; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D003587:Cytomegalovirus / Q000502:physiology; D015654:Herpesvirus 6, Human / Q000502:physiology*; D016199:Herpesvirus 7, Human / Q000502:physiology; D006801:Humans; D021381:Protein Transport; D018139:Simplexvirus / Q000502:physiology; D014764:Viral Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D014779:Virus Replication
D014764:Viral Proteins