title: Antimicrobial activity on glass materials subject to disinfectant xerogel coating.
abstract: The antimicrobial compound dodecyl-di(aminoethyl)-glycine was immobilized in a silicon oxide xerogel matrix and used for glass surface coating. Coated glasses were tested for surface antimicrobial activity. The utilization of tetraethoxysilane (TEOS) as a silicon oxide polymer precursor, using the dip-coating process, allowed for the generation of transparent thin films over glass surfaces. Different concentrations of the antimicrobial compound were used to generate the coatings. The presence of dodecyl-di(aminoethyl)-glycine on coated and uncoated slides was analyzed by FT-IR spectra. Coated glass slides were exposed to suspensions of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus for 24 h. Surface contamination was evaluated by the microbial plate count technique. When antimicrobial-coated glasses were compared with antimicrobial-free coated glasses, the former showed greater than 99% reduction of colony-forming units (cfu) for E. coli and P. aeruginosa, when 1% of antimicrobial was present in the coating solution. The same percentage of reduction for S. aureus was achieved when 1.5% of the antimicrobial was present in the coating solution. In a direct inhibition test on agar plates, no inhibitory zone was observed, indicating that the antimicrobial did not diffuse into the media.
authors.affiliation: Cátedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires (UBA), Junín 956--Piso 3rd (1113), Ciudad de Buenos Aires, Argentina.
authors.forename: G J
authors.initials: GJ
authors.lastname: Copello
authors.forename: S
authors.initials: S
authors.lastname: Teves
authors.forename: J
authors.initials: J
authors.lastname: Degrossi
authors.forename: M
authors.initials: M
authors.lastname: D'Aquino
authors.forename: M F
authors.initials: MF
authors.lastname: Desimone
authors.forename: L E
authors.initials: LE
authors.lastname: Diaz
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D004202:Disinfectants / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects; D005898:Glass / Q000737:chemistry*; D005998:Glycine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012821:Silanes / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D004202:Disinfectants; D012821:Silanes; C040733:tetraethoxysilane; C004206:dodicin; D005998:Glycine