title: Enzyme-linked immunosorbent assays for detection of xylem-limited bacteria: use of trinder reagent.
abstract: Quantitation and detection of xylem-limited bacteria with an enzyme-linked immunosorbent assay with a peroxidase conjugate is described. The use of the Trinder reagent (4-aminoantipyrine) allows the determination of extremely small quantities of peroxidase with no precipitate formation or inactivation of the enzyme by H(2)O(2). Comparison of the enzyme-linked immunosorbent assay method with microscopic and histochemical tests for the presence of the phony peach disease bacterium in 9-year-old "June Gold" peach trees gave comparable results. The peroxidase conjugate with the Trinder reagent is more sensitive than the alkaline phosphatase conjugate typically used for enzyme-linked immunosorbent assay quantitation.
authors.affiliation: University of Florida, Agricultural Research Center, Monticello, Florida 32344.
authors.forename: M S
authors.initials: MS
authors.lastname: Saunders
authors.forename: W J
authors.initials: WJ
authors.lastname: French