title: Polymorphism of RANTES chemokine gene promoter is not associated with long-term nonprogressive HIV-1 infection of more than 16 years.
abstract: To examine whether polymorphisms of the RANTES chemokine gene promoter are associated with long-term nonprogressive HIV-1 infection in white Spanish subjects, we performed a cross-sectional genetic association case-control study. Two-hundred sixty-seven white Spaniards were studied: 58 were HIV-1-infected long-term nonprogressors (LTNPs) of more than 16 years, 109 were HIV-1-infected usual progressors (UPs), and 100 were control subjects. Three RANTES single nucleotide polymorphisms (SNPs) at positions -28C>G, -109T>C, and -403G>A were assessed. The prevalence of the CCR5Delta 32 allele was also examined. Genotyping was performed using polymerase chain reaction and automatic sequencing analysis methods. Genotype and allele frequencies between the 3 groups were compared by the chi2 test and the Fisher exact test. The distribution of allelic variants of RANTES in controls, UPs, and LTNPs, respectively, was 3%, 2%, and 5% for -28G; 4%, 2%, and 2% for -109C; and 18%, 18%, and 18% for -403A (P = not significant). The differences were still nonsignificant when we exclusively analyzed individuals not carrying the CCR5Delta32 allele. We conclude that LTNP of more than 16 years is not associated with SNPs in the RANTES gene promoter in white Spanish HIV-1-infected subjects.
authors.affiliation: Hospital Universitari de Tarragona Joan XXIII, Universitat Rovira i Virgili, Tarragona, Spain. fvidal@comt.es
authors.forename: Francesc
authors.initials: F
authors.lastname: Vidal
authors.forename: Joaquim
authors.initials: J
authors.lastname: Peraire
authors.forename: Pere
authors.initials: P
authors.lastname: Domingo
authors.forename: Montserrat
authors.initials: M
authors.lastname: Broch
authors.forename: Mireia
authors.initials: M
authors.lastname: Cairó
authors.forename: Enric
authors.initials: E
authors.lastname: Pedrol
authors.forename: Milagros
authors.initials: M
authors.lastname: Montero
authors.forename: Consuelo
authors.initials: C
authors.lastname: Viladés
authors.forename: Cristina
authors.initials: C
authors.lastname: Gutiérrez
authors.forename: María Antònia
authors.initials: MA
authors.lastname: Sambeat
authors.forename: Angels
authors.initials: A
authors.lastname: Fontanet
authors.forename: David
authors.initials: D
authors.lastname: Dalmau
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Deig
authors.forename: Hernando
authors.initials: H
authors.lastname: Knobel
authors.forename: Joan Josep
authors.initials: JJ
authors.lastname: Sirvent
authors.forename: Cristóbal
authors.initials: C
authors.lastname: Richart
authors.forename: Sergi
authors.initials: S
authors.lastname: Veloso
authors.forename: Maria
authors.initials: M
authors.lastname: Saumoy
authors.forename: Miguel
authors.initials: M
authors.lastname: López-Dupla
authors.forename: Montserrat
authors.initials: M
authors.lastname: Olona
authors.forename: Josep
authors.initials: J
authors.lastname: Cadafalch
authors.forename: Montserrat
authors.initials: M
authors.lastname: Fuster
authors.forename: Anna
authors.initials: A
authors.lastname: Ochoa
authors.forename: Anna
authors.initials: A
authors.lastname: Soler
authors.forename: Ana
authors.initials: A
authors.lastname: Guelar
authors.forename: Judit
authors.initials: J
authors.lastname: González
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000235:genetics*; D018925:Chemokines / Q000235:genetics; D003430:Cross-Sectional Studies; D018450:Disease Progression; D005260:Female; D015658:HIV Infections / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D013030:Spain; D013997:Time Factors
chemical_list: D018946:Chemokine CCL5; D018925:Chemokines