title: Improved fixation of cellulose-acetate reverse-osmosis membrane for scanning electron microscopy.
abstract: Fixation of cellulose-acetate membranes with either glutaraldehyde-osmium tetroxide or glutaraldehyde-ruthenium tetroxide resulted in extensive electron beam damage. Beam damage was eliminated and the bacterial surface structure was preserved, however, when cellulose-acetate membranes were fixed with glutaraldehyderuthenium tetroxide and treated successively with thiocarbohydrazide and osmium tetroxide.
authors.affiliation: Department of Microbiology and Immunology and Electron Microscope Facility, College of Agriculture, University of Arizona, Tucson, Arizona 85721.
authors.forename: S M
authors.initials: SM
authors.lastname: Kutz
authors.forename: D L
authors.initials: DL
authors.lastname: Bentley
authors.forename: N A
authors.initials: NA
authors.lastname: Sinclair