title: Nitroaromatics Are Substrates for the TOL Plasmid Upper-Pathway Enzymes.
abstract: Expression of the xylMA genes encoding for toluene monoxygenase from the lactose promoter in a broad-host-range plasmid allows the oxidation of toluene and m- and p-nitrotoluene to their corresponding benzyl alcohols and benzaldehydes in Pseudomonas putida and Escherichia coli. Benzyl alcohols accumulate until reaching a concentration of about 80 muM, while benzaldehydes accumulate steadily with time for at least 24 h. TOL-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase recognize m- and p-nitro-substituted compounds as substrates. In contrast, the XylR protein, which regulates the TOL plasmid-encoded upper-pathway operon, does not recognize nitro-substituted toluenes as effectors.
authors.affiliation: Departamento de Bioquímica Vegetal, Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Apto. 419, 18080 Granada, Spain, and Department of Biochemistry, University of Groningen, Groningen, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Delgado
authors.forename: M G
authors.initials: MG
authors.lastname: Wubbolts
authors.forename: M A
authors.initials: MA
authors.lastname: Abril
authors.forename: J L
authors.initials: JL
authors.lastname: Ramos