title: Improved cell dispersal method by low-temperature incubation of tissue in a trypsin-citrate solution.
abstract: Tissues soaked at low temperature in a trypsin-citrate solution, washed to remove enzymes, and disrupted by rapid mechanical vibrations gave yields of viable cells 1.6 to 5 times those from conventional procedures.
authors.affiliation: Rijks Instituut voor de Volksgezondheid, Postbus 1, Bilthoven, The Netherlands.
authors.forename: A L
authors.initials: AL
authors.lastname: van Wezel
authors.forename: P A
authors.initials: PA
authors.lastname: van Hemert
authors.forename: W
authors.initials: W
authors.lastname: Parisius
authors.forename: N B
authors.initials: NB
authors.lastname: Cucakovich
authors.forename: H G
authors.initials: HG
authors.lastname: Macmorine