Effect of supplementing terpenoid biosynthetic precursors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures.
The effect of precursor feeding on the production of bilobalide and ginkgolides was studied with suspension cell cultures of Ginkgo biloba. The precursors greatly influenced the productivity of bilobalide and ginkgolides. Precursor supplementation increased the accumulation of both bilobalide and ginkgolides, and with positive effect on cell growth. The GA accumulation by cell cultures was influenced by precursors upstream in the metabolism, whereas the BB accumulation was under the influence of downstream precursors of the terpenoid biosynthetic pathway. Furthermore, precursor feeding modified the ratios of the BB, GA and GB in cells and cell cultures of G. biloba. The studies also aid in understanding effect of precursor feeding on the bilobalide and ginkgolides biosynthetic pathway.
Kang
Seung-Mi
SM
Division of Forest Science, Gyeongsang National University, Jinju 660-701, South Korea.
Min
Ji-Yun
JY
Kim
Yong-Duck
YD
Park
Dong-Jin
DJ
Jung
Ha-Na
HN
Karigar
Chandrakant S
CS
Ha
Yeong-Lae
YL
Kim
Seon-Won
SW
Choi
Myung-Suk
MS
D046935:Bilobalides / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism* / Q000494:pharmacology; D020441:Ginkgo biloba / Q000187:drug effects* / Q000378:metabolism*; D046934:Ginkgolides / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism* / Q000494:pharmacology
D046935:Bilobalides; D017077:Culture Media, Conditioned; D046934:Ginkgolides; D013729:Terpenes