title: Enzymatic preparation of maltohexaose, maltoheptaose, and maltooctaose by the preferential cyclomaltooligosaccharide (cyclodextrin) ring-opening reaction of Pyrococcus furiosus thermostable amylase.
abstract: Specific-length maltooligosaccharides, particularly maltohexaose, maltoheptaose, and maltooctaose, were prepared from cyclomaltooligosaccharides (cyclodextrins, CDs) by the preferential cyclodextrin ring-opening reaction of an amylolytic enzyme from Pyrococcus furiosus. The enzyme primarily produces maltohexaose, maltoheptaose, and maltooctaose by hydrolyzing alpha-, beta-, and gamma-CD, respectively. This study aims to develop a high-efficiency synthesis of specific maltooligosaccharides at high-purity. [formula: see text]
authors.affiliation: Center for Agricultural Biomaterials and Department of Food and Animal Biotechnology, School of Agricultural Biotechnology, Seoul National University, Shillim-dong, Kwanak-gu, Seoul 151-921, Republic of Korea.
authors.forename: Sung-Jae
authors.initials: SJ
authors.lastname: Yang
authors.forename: Hee-Seob
authors.initials: HS
authors.lastname: Lee
authors.forename: Jung-Woo
authors.initials: JW
authors.lastname: Kim
authors.forename: Myoung-Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Joong-Hyuck
authors.initials: JH
authors.lastname: Auh
authors.forename: Byong-Hoon
authors.initials: BH
authors.lastname: Lee
authors.forename: Kwan-Hwa
authors.initials: KH
authors.lastname: Park
mesh_terms: D000681:Amylases / Q000737:chemistry / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003505:Cyclodextrins / Q000378:metabolism*; D004795:Enzyme Stability; D005936:Glucans / Q000302:isolation & purification / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D009844:Oligosaccharides / Q000302:isolation & purification / Q000378:metabolism*; D020027:Pyrococcus furiosus / Q000201:enzymology*
chemical_list: D003505:Cyclodextrins; D005936:Glucans; D009844:Oligosaccharides; C016549:maltohexaose; C031146:maltoheptaose; C101183:maltooctaose; D000681:Amylases