A comparative study of the effects of hemin and bilirubin on bilateral renal ischemia reperfusion injury.
OBJECTIVE
The aim of this study was to determine the effects of hemin, a heme oxygenase-1 inducer, and bilirubin on renal ischemia-reperfusion (I-R) injury.


METHODS
40 Wistar-Albino rats were allocated into six groups as follows: sham (S), bilirubin (B), hemin (H), ischemia/reperfusion (IR), IR + bilirubin (IRB) and IR + hemin (IRH). Conjugated bilirubin (20 mg.kg(-1) i.v.) was given to rats in groups B and IRB, and hemin (50 mg.kg(-1) i.p.) was given to rats in groups H and IRH just prior to reperfusion. Renal I-R was achieved by occluding the renal arteries bilaterally for 50 min. Following 6 h of reperfusion, blood was drawn to study BUN, creatinine and bilirubin, and tissue samples were harvested to determine the renal malonyldialdehyde and heme oxygenase-1 levels, and for histopathologic grading.


RESULTS
BUN, creatinine and malonyldialdehyde levels in group IRH were similar to controls whereas the results of groups IR and IRB were significantly higher (p < 0.01). There was a grade 2 damage in all I-R groups.


CONCLUSIONS
This study showed the preventive effect of hemin on renal ischemia reperfusion injury. Administration of exogenous bilirubin did not prevent the I-R injury.
Demirogullari
Billur
B
Department of Pediatric Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey. billurd@yahoo.com
Ekingen
Gulsen
G
Guz
Galip
G
Bukan
Neslihan
N
Erdem
Ozlem
O
Ozen
I Onur
IO
Memis
Leyla
L
Sert
Sevki
S
D000818:Animals; D001663:Bilirubin / Q000097:blood / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006427:Hemin / Q000494:pharmacology*; D007668:Kidney / Q000098:blood supply*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000187:drug effects; D015427:Reperfusion Injury / Q000503:physiopathology*
D006427:Hemin; D003404:Creatinine; D051547:Heme Oxygenase-1; D001663:Bilirubin