A novel experimental approach for systematic identification of box H/ACA snoRNAs from eukaryotes.
Box H/ACA snoRNAs represent an abundant group of small non-coding RNAs mainly involved in the pseudouridylation of rRNAs and/or snRNAs in eukaryotes and Archaea. In this study, we describe a novel experimental method for systematic identification of box H/ACA snoRNAs from eukaryotes. In the specialized cDNA libraries constructed by this method with total cellular RNAs from human blood cells, the high efficiency of cloning for diverse box H/ACA snoRNAs was achieved and seven novel species of this snoRNA family were identified from human for the first time. Furthermore, the novel method has been successfully applied for the identification of the box H/ACA snoRNAs from Drosophila and the fission yeast, demonstrating a powerful ability for systematic analysis of box H/ACA snoRNAs in a broad spectrum of eukaryotes.
Gu
Ai-Di
AD
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Zhongshan University, Guangzhou 510275, People's Republic of China.
Zhou
Hui
H
Yu
Chun-Hong
CH
Qu
Liang-Hu
LH
D000818:Animals; D001483:Base Sequence; D001773:Blood Cells / Q000737:chemistry; D017931:DNA Primers; D004331:Drosophila melanogaster / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D020537:RNA, Small Nucleolar / Q000032:analysis* / Q000737:chemistry; D012568:Schizosaccharomyces / Q000235:genetics; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D020537:RNA, Small Nucleolar