title: Simultaneous determination of amoxicillin and ranitidine in rat plasma by high-performance liquid chromatography.
abstract: A high-performance liquid chromatography method using ultraviolet detection at 230 nm for the simultaneous determination of amoxicillin and ranitidine in rat plasma has been validated. Plasma samples after pretreatment with acetonitrile to effect deproteinization were dried under N2 and reconstituted with water. The standard calibration curves for amoxicillin and ranitidine were linear (r2=0.9999) over the concentration range of 0.2-20 microg ml-1 and 0.03-6 microg ml-1 in rat plasma, respectively. The intra- and inter-day assay variability range for amoxicillin was 2.4-8.5% and 3.2-11.7%, and for ranitidine was 1.7-9.0% and 4.5-10.1%, respectively. This method has been successfully applied to a pharmacokinetic study after oral coadministration of amoxicillin and ranitidine to rats.
authors.affiliation: The College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Fengping
authors.initials: F
authors.lastname: Tan
authors.forename: Kang
authors.initials: K
authors.lastname: Zhao
mesh_terms: D000658:Amoxicillin / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006635:Histamine H2 Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D011899:Ranitidine / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D006635:Histamine H2 Antagonists; D000658:Amoxicillin; D011899:Ranitidine