title: Spectral domain optical coherence tomography: ultra-high speed, ultra-high resolution ophthalmic imaging.
abstract: OBJECTIVE
  To introduce a new ophthalmic optical coherence tomography technology that allows unprecedented simultaneous ultra-high speed and ultra-high resolution.
  
  
  METHODS
  Using a superluminescent diode source, a clinically viable ultra-high speed, ultra-high resolution spectral domain optical coherence tomography system was developed.
  
  
  RESULTS
  In vivo images of the retina, the optic nerve head, and retinal blood flow were obtained at an ultra-high speed of 34.1 microseconds (ms) per A-scan, which is 73 times faster than commercially available optical coherence tomography instruments. Single images (B-scans) consisting of 1000 A-scans were acquired in 34.1 ms, allowing video rate imaging at 29 frames per second with an axial resolution of 6 mum. Using a different source in a slightly slower configuration, single images consisting of 500 A-scans were acquired in 34 ms, allowing imaging at 29 frames per second at an axial resolution of 3.5 microm, which is 3 times better than commercially available optical coherence tomography instruments. The amount of energy directed into the eye in both cases, 600 microW, is less than that of the Stratus OCT3 and is safe for intrabeam viewing for up to 8 hours at the same retinal location.
  
  
  CONCLUSIONS
  Spectral domain optical coherence tomography technology enables ophthalmic imaging with unprecedented simultaneous ultra-high speed and ultra-high resolution.
authors.affiliation: Department of Ophthalmology, Harvard Medical School, Boston, Mass, USA. teresa_chen@meei.harvard.edu
authors.forename: Teresa C
authors.initials: TC
authors.lastname: Chen
authors.forename: Barry
authors.initials: B
authors.lastname: Cense
authors.forename: Mark C
authors.initials: MC
authors.lastname: Pierce
authors.forename: Nader
authors.initials: N
authors.lastname: Nassif
authors.forename: B Hyle
authors.initials: BH
authors.lastname: Park
authors.forename: Seok H
authors.initials: SH
authors.lastname: Yun
authors.forename: Brian R
authors.initials: BR
authors.lastname: White
authors.forename: Brett E
authors.initials: BE
authors.lastname: Bouma
authors.forename: Guillermo J
authors.initials: GJ
authors.lastname: Tearney
authors.forename: Johannes F
authors.initials: JF
authors.lastname: de Boer
mesh_terms: D000328:Adult; D003941:Diagnostic Techniques, Ophthalmological; D006801:Humans; D008297:Male; D009898:Optic Disk / Q000033:anatomy & histology*; D012039:Regional Blood Flow; D012160:Retina / Q000033:anatomy & histology*; D012171:Retinal Vessels / Q000033:anatomy & histology*; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*