Spectral unraveling by space-selective Hadamard spectroscopy.
Spectral unraveling by space-selective Hadamard spectroscopy (SUSHY) enables recording of NMR spectra of multiple samples loaded in multiple sample tubes in a modified spinner turbine and a regular 5mm liquids NMR probe equipped with a tri-axis pulsed field gradient coil. The individual spectrum from each sample is extracted by adding and subtracting data that are simultaneously obtained from all the tubes based on the principles of spatially resolved Hadamard spectroscopy. The well-known Hadamard spectroscopy has been applied for spatial selection and the method utilizes standard configuration of NMR instrument hardware. The SUSHY method can be easily incorporated in multi-dimensional multi-tube NMR experiments. This method combines the excitation multiplexing, natural advantage of FTNMR, and sample multiplexing and offers high-throughput by reducing the total experimental time by up to a factor of four in a 4-tube mode.
Murali
Nagarajan
N
Varian Inc., 3120 Hansen Way, Palo Alto, CA 94304, USA. nagarajan.murali@varianinc.com
Miller
William Marcus
WM
John
Boban K
BK
Avizonis
Daina A
DA
Smallcombe
Stephen H
SH
D004867:Equipment Design; D005947:Glucose / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D013752:Tetracycline / Q000737:chemistry*
D013752:Tetracycline; D005947:Glucose