Development of an analytical method using microchip capillary electrophoresis for the measurement of fluorescein-labeled salivary components in response to exercise stress.
We have developed an analytical method using microchip capillary electrophoresis (microchip CE) for the high-speed separation of fluorescein-labeled salivary components in response to exercise stress. Optimal separation was obtained using a borate buffer at pH 9.5 containing 10 mM beta-cyclodextrin and 1.0% (w/v) methylcellulose. To minimize individual differences in human saliva, such as viscosity, conductivity, and contaminants, the concentration of methylcellulose in the analytical conditions played a key factor. The optimized separation conditions produced identical electropherograms successfully despite of the use of different microchips made from quartz glass or poly-methylmethacrylate (PMMA). In addition, a practical application of bicycle ergometer stress was performed. Some components in human saliva showed a marked decrease after exercise stress.
Tanaka
Yoshihide
Y
Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology (AIST) 1-8-31, Midorigaoka, Ikeda, Osaka 563-8577, Japan. yoshihide-tanaka@aist.go.jp
Naruishi
Nahoko
N
Nakayama
Yusuke
Y
Higashi
Tetsuji
T
Wakida
Shin-ichi
S
D046209:Electrophoresis, Microchip / Q000379:methods*; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D019793:Fluorescein / Q000737:chemistry; D006801:Humans; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D013312:Stress, Physiological / Q000503:physiopathology*
D019793:Fluorescein