Cloning and characterization of the goadsporin biosynthetic gene cluster from Streptomyces sp. TP-A0584.
The biosynthetic gene cluster of goadsporin, a polypeptide antibiotic containing thiazole and oxazole rings, was cloned from Streptomyces sp. TP-A0584. The cluster contains a structural gene, godA, and nine god (goadsporin) genes involved in post-translational modification, immunity and transcriptional regulation. Although the gene organization is similar to typical bacteriocin biosynthetic gene clusters, each goadsporin biosynthetic gene shows low homology to these genes. Goadsporin biosynthesis is initiated by the translation of godA, and the subsequent cyclization, dehydration and acetylation are probably catalysed by godD, godE, godF, godG and godH gene products. godI shows high similarity to the 54 kDa subunit of the signal recognition particle and plays an important role in goadsporin immunity. Furthermore, four goadsporin analogues were produced by site-directed mutagenesis of godA, suggesting that this biosynthesis machinery is used for the heterocyclization of peptides.
Onaka
Hiroyasu
H
Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan.
Nakaho
Mizuho
M
Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan.
Hayashi
Keiko
K
Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan.
Igarashi
Yasuhiro
Y
Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan.
Furumai
Tamotsu
T
Biotechnology Research Center, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan.
D003001:Cloning, Molecular; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D010455:Peptides / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011994:Recombinant Proteins; C449027:goadsporin