title: High Efficiency of Plating of the Thermophilic Sulfur-Dependent Archaebacterium Sulfolobus acidocaldarius.
abstract: A procedure for plating Sulfolobus acidocaldarius using Gelrite as the gelling agent is presented. The technique uses a supporting gel of 0.8% (wt/vol) Gelrite and an overlay soft gel of 0.4% (wt/vol) Gelrite, in which the colonies are grown. The plating efficiency was essentially 100%.
authors.affiliation: Unit for Applied Cell and Molecular Biology, University of Umeå, S-901 87 Umeå, Sweden.
authors.forename: E B
authors.initials: EB
authors.lastname: Lindström
authors.forename: H M
authors.initials: HM
authors.lastname: Sehlin