title: Multiphasic kinetics for transformation of methyl parathion by flavobacterium species.
abstract: Transformation rates of an insecticide, methyl parathion, in pure cultures of Flavobacterium sp. followed multiphasic kinetics involving at least two systems (I and II). System I was a high-affinity, low-capacity system, and system II was a low-affinity, high-capacity system. Data from rate experiments suggested that metabolites formed via system II inhibited system I such that only one system operated at a time. System I operated at approximately 20 mug liter and less; system II operated at approximately 4 mg liter and less. These results show that xenobiotic chemicals, like naturally occurring substrates, can be transformed via multiple uptake and transformation systems even by a pure culture. Furthermore, computer simulation models of pollutant transformation rates based on kinetic constants determined in this study show that large errors can occur in predicted rates when the multiphasicity of kinetics is neglected.
authors.affiliation: Environmental Research Laboratory, U.S. Environmental Protection Agency, Athens, Georgia 30613, and Department of Microbiology and Institute of Ecology, University of Georgia, Athens, Georgia 30602.
authors.forename: D L
authors.initials: DL
authors.lastname: Lewis
authors.forename: R E
authors.initials: RE
authors.lastname: Hodson
authors.forename: L F
authors.initials: LF
authors.lastname: Freeman