Preparation and antibacterial test of chitosan/PAA/PEGDA bi-layer composite membranes.
Chitosan/poly(acrylic acid)/poly(ethylene glycol) diacrylate (PEGDA) composite membranes with a bi-layer configuration were prepared and their potential application as an antibacterial material was examined. A two-step process was adopted. A dope consisting of PEGDA, acrylic acid (AA) and a photoinitiator was cast and then UV-cured on a glass substrate to form a mechanically strong, dense membrane. Subsequently, the membrane was coated with a layer of solution composed of chitosan, AA and water. As the majority of AA diffused downwards into the supporting layer underneath, chitosan coagulated with residual AA to form a nano-layer on the top surface by means of UV irradiation. Low-voltage field-emission scanning electron microscopy was used to observe the membrane morphology and to measure the thickness of the top layer. Contact angle measurements indicated a top layer mixed with chitosan and poly(acrylic acid), which was confirmed by chemical composition analysis of X-ray photon spectroscopy. The antibacterial activities of the formed membranes were tested both with respect to a Gram-negative (Escherichia coli) and a Gram-positive (Staphylococcus aureus) bacteria.
Don
Trong-Ming
TM
Department of Chemical and Materials Engineering, Tamkang University, Taipei, Taiwan, ROC 25137.
Chen
Ching-Chung
CC
Lee
Chih-Kang
CK
Cheng
Wei-Yuan
WY
Cheng
Liao-Ping
LP
D000180:Acrylic Resins / Q000737:chemistry* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology; D013052:Spectrometry, X-Ray Emission; D013211:Staphylococcus aureus / Q000187:drug effects
D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D008567:Membranes, Artificial; D011092:Polyethylene Glycols; C006903:carbopol 940; D048271:Chitosan