Redox and redox-coupled processes of heme proteins and enzymes at electrochemical interfaces.
Modern bioelectrochemical methods rely upon the immobilisation of redox proteins and enzymes on electrodes coated with biocompatible materials to prevent denaturation. However, even when protein denaturation is effectively avoided, heterogeneous protein electron transfer is often coupled to non-Faradaic processes like reorientation, conformational transitions or acid-base equilibria. Disentangling these processes requires methods capable of probing simultaneously the structure and reaction dynamics of the adsorbed species. Here we provide an overview of the recent developments in Raman and infrared surface-enhanced spectroelectrochemical techniques applied to the study of soluble and membrane bound redox heme proteins and enzymes. Possible biological implications of the findings are critically discussed.
Murgida
Daniel H
DH
Technische Universität Berlin, Institut für Chemie, Max-Volmer-Laboratorium für Biophysikalische Chemie, Sekr. PC14, Strasse des 17. Juni 135, D-10623, Berlin, Germany. dh.murgida@tu-berlin.de
Hildebrandt
Peter
P
D015374:Biosensing Techniques / Q000295:instrumentation*; D004563:Electrochemistry; D006420:Hemeproteins / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000502:physiology*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation
D006420:Hemeproteins; D008565:Membrane Proteins; D010088:Oxidoreductases