title: A derivative of Mycobacterium smegmatis mc(2)155 that lacks the duplicated chromosomal region.
abstract: The genome of Mycobacterium smegmatis mc(2)155 contains a 56kb duplicated region. We isolated a mutant of mc(2)155 lacking this duplication (DeltaDRKIN). This mutation did not affect the growth rate, surface properties or transformation efficiency of the organism, confirming the potential utility of DeltaDRKIN for the study of genes contained within the duplicated region.
authors.affiliation: MRC/NHLS/WITS Molecular Mycobacteriology Research Unit, DST-NRF, School of Pathology of the University of the Witwatersrand, Johannesburg, South Africa. digby.warner@nhls.ac.za <digby.warner@nhls.ac.za>
authors.forename: Digby F
authors.initials: DF
authors.lastname: Warner
authors.forename: Gilles
authors.initials: G
authors.lastname: Etienne
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Wang
authors.forename: Limenako G
authors.initials: LG
authors.lastname: Matsoso
authors.forename: Stephanie S
authors.initials: SS
authors.lastname: Dawes
authors.forename: Karine
authors.initials: K
authors.lastname: Soetaert
authors.forename: Neil G
authors.initials: NG
authors.lastname: Stoker
authors.forename: Jean
authors.initials: J
authors.lastname: Content
authors.forename: Valerie
authors.initials: V
authors.lastname: Mizrahi
mesh_terms: D020440:Gene Duplication; D005798:Genes, Bacterial; D020102:Mycobacterium smegmatis / Q000235:genetics* / Q000254:growth & development; D016366:Open Reading Frames; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D013499:Surface Properties; D014170:Transformation, Genetic