Purification and characterization of a cold-active iron superoxide dismutase from a Psychrophilic Bacterium, Marinomonas sp. NJ522.
Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 degrees C (assayed over 10 min). Activity at 0 degree C was nearly 35% of the maximum activity.
Zheng
Zhou
Z
College of life Sciences, Ocean University of China, Qingdao.
Jiang
Ying-Hui
YH
Miao
Jin-Lai
JL
Wang
Quan-Fu
QF
Zhang
Bo-Tao
BT
Li
Guang-You
GY
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D003080:Cold Temperature; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D013482:Superoxide Dismutase / Q000737:chemistry / Q000302:isolation & purification*
D001426:Bacterial Proteins; D013482:Superoxide Dismutase