title: Microarray-based gene expression profiling of retransplanted rat cardiac allografts that develop cardiac allograft vasculopathy.
abstract: We studied the expression of 9,906 genes in retransplanted rat cardiac allografts that developed cardiac allograft vasculopathy (CAV) with the use of DNA microarray and real-time reverse transcriptase-polymerase chain reaction. Although only a slight difference in the timing of the retransplantation induced the later development of CAV, 1,067 genes were differentially expressed in the allografts 1 day after retransplantation. Thus, the development of CAV was determined by a robust difference in gene expression soon after retransplantation, controlled by a slight difference in retransplantation timing. In contrast, only 26 genes showed significant upregulation in the later phase of CAV development, and the time-course of the induction of 16 genes was associated with CAV progression. Of these genes, 8 were induced in 2 different aortic allograft combinations, and the time-course of the induction was correlated with the development of transplant vasculopathy. Microarray-based gene expression profiling has the potential to elucidate the mechanism of experimental chronic cardiac allograft rejection.
authors.affiliation: Department of Molecular Pharmacology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan. sakakida@surg1.med.osaka-u.ac.jp
authors.forename: Satoru
authors.initials: S
authors.lastname: Kitagawa-Sakakida
authors.forename: Norihide
authors.initials: N
authors.lastname: Fukushima
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
authors.forename: Kei
authors.initials: K
authors.lastname: Horiguchi
authors.forename: Takefumi
authors.initials: T
authors.lastname: Doi
authors.forename: Ryota
authors.initials: R
authors.lastname: Shirakura
authors.forename: Hikaru
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D006084:Graft Rejection / Q000235:genetics* / Q000503:physiopathology*; D016027:Heart Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D012086:Reoperation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D016042:Transplantation, Heterotopic; D014184:Transplantation, Homologous; D014652:Vascular Diseases / Q000209:etiology / Q000235:genetics* / Q000503:physiopathology