Binding of alpha1-antichymotrypsin to the surface of lymphocytes--preliminary study.
OBJECTIVE
The paper presents preliminary results of investigation of binding of alpha1-antichymotrypsin to the surface of peripheral blood lymphocytes.


METHODS
Pooled serum samples from healthy individuals served as source of alpha1-antichymotrypsin for isolation using chromatography. Binding of alpha1-antichymotrypsin to the surface of peripheral blood lymphocytes was measured by flow cytometry.


RESULTS
Even on native cells alpha1-antichymotrypsin may be detected. After incubation with isolated preparation the percentage of positive lymphocytes increased.


CONCLUSIONS
The presence of alpha1-antichymotrypsin on the surface of lymphocytes may imply its regulatory role during acute phase response and early immune response.
Sobieska
M
M
Department of Physiotherapy, Rheumatology and Rehabilitation, Karol Marcinkowski University of Medical Sciences, Poznań, Poland. magdula2811@poczta.onet.pl
D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D011485:Protein Binding; D015842:Serine Proteinase Inhibitors / Q000378:metabolism*; D000514:alpha 1-Antichymotrypsin / Q000378:metabolism*
D015842:Serine Proteinase Inhibitors; D000514:alpha 1-Antichymotrypsin