Effect of Calcium Cation on Plating Efficiency of the Sulfate-Reducing Bacterium Desulfovibrio vulgaris.
The presence of Ca (concentration, ca. 0.4 mM) in the growth medium causes cells of Desulfovibrio vulgaris (Hildenborough) to aggregate, leading to a decrease in plating efficiency. When the Ca concentration in the medium was reduced 20-fold, cell aggregation did not occur and the plating efficiency increased from an initial value of 34% to a final value of 56%.
Singleton
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School of Life and Health Sciences, University of Delaware, Newark, Delaware 19716.
Ketcham
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Campbell
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