[Synthesis, characterization and evaluation of octadecyl ester-bonded monolithic column].
An ester-bonded monolithic silica column for reversed-phase high performance liquid chromatography was synthesized by reacting stearic acid with gamma-glycidoxypropyltrimethoxysilane and coupling the intermediate product onto monolithic silica column. Characterization of the prepared column was carried out with Fourier transformation infrared spectrometry and elemental analysis. The chromatographic properties of the packing were evaluated by using benzene, biphenyl, and anthracene as samples and methanol-water as binary mobile phase. The column back-pressure and separation effect were also evaluated for fast separation. The results show that the stationary phase has satisfactory chromatographic properties and resists hydrolysis between pH = 2 - 8, and it can be used for the fast-separation of solutes efficiently.
Wang
Lijuan
L
College of Pharmacy, Hebei University, Baoding 071002, China.
Yang
Gengliang
G
Li
Xu
X
Li
Xiaoyu
X
Yin
Junfa
J
Li
Zhiwei
Z
Gao
Wenhui
W
Yang
Jing
J
Chen
Yi
Y
D000873:Anthracenes / Q000032:analysis; D001554:Benzene / Q000032:analysis; D001713:Biphenyl Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D004952:Esters / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D012821:Silanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013229:Stearic Acids / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000873:Anthracenes; D001554:Benzene; D001713:Biphenyl Compounds; D004952:Esters; D000432:Methanol; D012821:Silanes; D013229:Stearic Acids; D014867:Water; C039462:gamma-glycidoxypropyltrimethoxysilane; C010574:biphenyl; C031183:stearic acid; C034020:anthracene