title: Enzymatic and Microbial Preparation of d-Xylulose from d-Xylose.
abstract: A high-d-xylulose mixture (d-xylose-d-xylulose = 33:67) was prepared from the cold ethanol extract of preisomerized d-xylose solution (d-xylose-d-xylulose = 77:23). Fusarium oxysporum f. sp. lini and Aspergillus niger were demonstrated to preferentially utilize d-xylose in the mixture of d-xylose and d-xylulose. Chromatographically pure d-xylulose was thus obtained in 90% yield. A high-d-xylulose mixture was also incubated with Rhodotorula toruloides, Klebsiella pneumoniae, Candida utilis, or Mucor rouxii.d-Xylose and d-xylulose were simultaneously consumed. When borate was added to the mixture, a d-xylulose-borate complex was formed, and it could be used to protect d-xylulose from being utilized.
authors.affiliation: Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana 47907.
authors.forename: L C
authors.initials: LC
authors.lastname: Chiang
authors.forename: H Y
authors.initials: HY
authors.lastname: Hsiao
authors.forename: P P
authors.initials: PP
authors.lastname: Ueng
authors.forename: G T
authors.initials: GT
authors.lastname: Tsao