title: [Expression of B-glucosidase gene of Sacchromycopsis fibuligera in Pichia pastoris].
abstract: A beta-Glucosidase gene (BGL 1) was amplified with PCR from the total DNA of Sacchromycopsis fibuligera, and was linked with pGEM-T vector. After cut down by restriction enzyme from pGEM-T vector, BGL 1 was inserted into the expression vector pPIC9K of Pichia pastoris in reading frame with alpha-factor secreting signal peptide sequence to construct the recombinant plasmid pSHL9K. The recombinant plasmid pSHL9K was transformed into Pichia pastoris GS115 with electroporation. The recombinant Pichia pastoris strains which could efficiently secret recombinant beta-Glucosidase were selected. The optimum temperature of the recombinant beta-Glucosidase was 50degreesC, and the optimum pH was 5.4. The activity of beta-Glucosidase could reach to 47U/mL in the culture medium.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences, Beijing.
authors.forename: Jin-hui
authors.initials: JH
authors.lastname: Shao
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ai-jun
authors.initials: AJ
authors.lastname: Mao
authors.forename: Ya-xin
authors.initials: YX
authors.lastname: Zhu
authors.forename: Zhi-yang
authors.initials: ZY
authors.lastname: Dong
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
mesh_terms: D010843:Pichia / Q000235:genetics*; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D012442:Saccharomycopsis / Q000201:enzymology* / Q000235:genetics; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D001617:beta-Glucosidase