title: Low titer maternal antibodies can both enhance and suppress B cell responses to a combined live attenuated human rotavirus and VLP-ISCOM vaccine.
abstract: We investigated effects of low titer (Lo) circulating MatAb on protection and immunogenicity of attenuated (Att) human rotavirus (HRV) priming and 2/6-virus-like particle (VLP)-immunostimulating complex (ISCOM) boosting (AttHRV/VLP) or VLP-ISCOM alone vaccines. LoMatAb had both enhancing and suppressing effects on B cell responses, depending on tissue, antibody isotype and vaccine. Differential effects of LoMatAb on IgA responses in different tissues suggest that LoMatAb did not suppress induction of IgA effector and memory B cells but impaired homing of these cells to secondary lymphoid or effector tissues, reducing IgA antibody secreting cells and antibodies at these sites. The AttHRV/VLP vaccine partially overcame LoMatAb suppression, conferred moderate protection against virulent HRV (as measured by reduced viral shedding and diarrhea) and represents a new candidate for rotavirus vaccines for both humans and animals.
authors.affiliation: Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691-4096, USA.
authors.forename: Trang V
authors.initials: TV
authors.lastname: Nguyen
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yuan
authors.forename: Marli S P
authors.initials: MS
authors.lastname: Azevedo
authors.forename: Kwang-Il
authors.initials: KI
authors.lastname: Jeong
authors.forename: Ana M
authors.initials: AM
authors.lastname: Gonzalez
authors.forename: Cristiana
authors.initials: C
authors.lastname: Iosef
authors.forename: Karin
authors.initials: K
authors.lastname: Lovgren-Bengtsson
authors.forename: Bror
authors.initials: B
authors.lastname: Morein
authors.forename: Peggy
authors.initials: P
authors.lastname: Lewis
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Saif
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology*; D006801:Humans; D016882:ISCOMs / Q000008:administration & dosage*; D007112:Immunity, Maternally-Acquired; D007156:Immunologic Memory; D007274:Injections, Intraperitoneal; D022243:Rotavirus Vaccines / Q000276:immunology*; D013552:Swine; D014613:Vaccines, Attenuated / Q000276:immunology; D014771:Virion / Q000276:immunology*; D017201:Virus Shedding
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016882:ISCOMs; D022243:Rotavirus Vaccines; D014613:Vaccines, Attenuated