Viral reactivation and pseudotype production in an in vitro superinfection system with two different strains of HIV-1.
Viral production and variability of HIV-1 is normally high in vivo causing the necessary conditions for cellular superinfection. In order to evaluate the superinfection dynamics in vitro, H9HTLVIIIB cell line was superinfected with HIVMN. Superinfected cells showed nearly 50% cell mortality at day 1 post-superinfection (ps), which increased significantly up to day 4 ps. Superinfecting genome was detectable until day 10 ps. The superinfecting strain was found in the supernatant only on day 1 ps, but was recovered up to day 4 ps by coculture with non-infected cells. The existing strain (HIVHXB2) was recovered throughout the studied period. Pseudotype formation by the HIVHXB2 genome and envelope proteins of the superinfecting strain (HIVMN) was observed from day 1 to 6 ps. Viral production was increased by 1.7 LOG in superinfected cells from day 1 ps. Both viral production increase and pseudotype formation could be relevant for HIV pathogenesis in vivo.
Fernández Larrosa
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PN
National Reference Center for AIDS, Department of Microbiology, University of Buenos Aires, School of Medicine, Buenos Aires, Argentina. plarrosa@fmed.uba.ar
Ceballos
A
A
Andreani
G
G
Marquina
S
S
Martínez Peralta
L
L
Rabinovich
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RD
D002460:Cell Line; D018920:Coculture Techniques; D003588:Cytopathogenic Effect, Viral; D016679:Genome, Viral; D015483:HIV Antibodies / Q000276:immunology; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D009500:Neutralization Tests; D015163:Superinfection / Q000821:virology*; D013997:Time Factors; D014775:Virus Activation
D015483:HIV Antibodies