Chimpanzee adenovirus vaccine protects against Zaire Ebola virus.
This study evaluated the use of a chimpanzee-based adenovirus vaccine in mouse and Guinea pigs models of Zaire Ebola virus (ZEBOV) infection. Vaccine vector expressing the envelope glycoprotein of ZEBOV was created from the molecular clone of chimpanzee adenovirus pan7 (AdC7). AdC7 vaccine stimulated robust T and B cell responses to ZEBOV in naïve mice inducing complete protection to an otherwise lethal challenge of ZEBOV. Complete protection to Zaire Ebola virus was also observed in Guinea pigs vaccinated with a relatively low dose of AdC7 (5 x 10(9)/kg). Pre-existing immunity to AdHu5 was generated in mice following pre-exposure to AdHu5 or administration of pooled human immune globulin. Pre-existing immunity to human adenoviruses severely compromised the efficacy of the human AdHu5 vaccine but not the chimpanzee AdC7 vaccine. These results validate further development of Chimpanzee-based vaccine and highlight the impact of pre-existing immunity to the vaccine carrier.
Kobinger
Gary P
GP
Special Pathogens Program, National Microbiology Laboratory, Health Canada, Canadian Science Centre for Human and Animal Health, University of Manitoba, Winnipeg, Canada.
Feldmann
Heinz
H
Zhi
Yan
Y
Schumer
Gregory
G
Gao
Guangping
G
Feldmann
Friederike
F
Jones
Steven
S
Wilson
James M
JM
D000260:Adenoviruses, Human / Q000276:immunology; D000261:Adenoviruses, Simian / Q000235:genetics* / Q000276:immunology*; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001835:Body Weight; D004195:Disease Models, Animal; D046129:Ebola Vaccines / Q000008:administration & dosage / Q000276:immunology*; D029043:Ebolavirus / Q000235:genetics / Q000276:immunology*; D006168:Guinea Pigs; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology / Q000517:prevention & control*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D046129:Ebola Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C113189:envelope glycoprotein, Ebola virus