title: Identification of different agrobacterium strains isolated from the same forest nursery.
abstract: Several Agrobacterium strains isolated from the same forest nursery from 1982 to 1988 were compared by serological, biochemical, and DNA-DNA hybridization methods. Similarities among strains belonging to biovar 2 were observed by indirect immunofluorescence, whereas biovar 1 strains showed serological heterogeneity. Electrophoretic analysis of bacterial envelope-associated proteins showed that few bands appeared in the strains belonging to biovar 1, whereas many proteins appeared in the case of biovar 2 strains. Chromosomal DNA was analyzed with six random C58 chromosomal fragments. None of the six probes hybridized to the DNA of the two biovar 2 strains. One of the probes gave the same hybridization pattern with all biovar 1 strains, whereas the other probes yielded different patterns. The vir regions were closely related in the different pathogenic strains. The T-DNA and replication regions were less conserved and showed some variations among the strains.
authors.affiliation: Station d'Amélioration des Arbres Forestiers, Institut National de la Recherche Agronomique, Ardon, F-45160 Olivet.
authors.forename: M F
authors.initials: MF
authors.lastname: Michel
authors.forename: A C
authors.initials: AC
authors.lastname: Brasileiro
authors.forename: C
authors.initials: C
authors.lastname: Depierreux
authors.forename: L
authors.initials: L
authors.lastname: Otten
authors.forename: F
authors.initials: F
authors.lastname: Delmotte
authors.forename: L
authors.initials: L
authors.lastname: Jouanin