Denaturing high-performance liquid chromatography-based genotyping and genetic variation of FUT2 in Sri Lanka.
BACKGROUND
Human ABO-secretor locus (FUT2) is highly polymorphic in many human populations.


METHODS
The applicability of denaturing high-performance liquid chromatography (DHPLC) analysis was evaluated for genotyping the FUT2 in two Sri Lankan populations (Tamil and Sinhalese).


RESULTS
Although DHPLC failed to detect one allele, 302C>T, of eight alleles, this method reduces the number of samples to be sequenced and can detect novel polymorphisms by comparing the elution profiles.


CONCLUSIONS
These results suggest that DHPLC analysis is a useful high-throughput method for genotyping FUT2 and, further, that the genetic backgrounds of two Sri Lankan populations are quite similar, with little genetic flow from neighboring East and Southeast Asian populations to Sri Lanka.
Soejima
Mikiko
M
Department of Forensic Medicine and Human Genetics, Kurume University School of Medicine, Kurume, Japan.
Koda
Yoshiro
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005647:Fucosyltransferases / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods*; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D009691:Nucleic Acid Denaturation; D020641:Polymorphism, Single Nucleotide; D013188:Sri Lanka; D000097631:Galactoside 2-alpha-L-fucosyltransferase
D005647:Fucosyltransferases; D000097631:Galactoside 2-alpha-L-fucosyltransferase