Effects of streptozotocin-induced diabetes and physical training on gene expression of extracellular matrix proteins in mouse skeletal muscle.
Diabetes induces changes in the structure and function of the extracellular matrix (ECM) in many tissues. We investigated the effects of diabetes, physical training, and their combination on the gene expression of ECM proteins in skeletal muscle. Mice were divided to control (C), training (T), streptozotocin-induced diabetic (D), and diabetic training (DT) groups. Training groups (T, DT) performed 1, 3, or 5 wk of endurance training on a treadmill. Gene expression of calf muscles was analyzed using microarray and quantitative PCR. Training group samples were collected 24 h after the last training session. Diabetes affected the gene expression of several collagens (types I, III, IV, V, VI, and XV), some noncollagenous glycoproteins, and proteoglycans (e.g., elastin, thrombospondin-1, laminin-2, decorin). Reduced gene expression of collagens in diabetic skeletal muscle was partially attenuated as a result of physical training. In diabetes, mRNA expression of the basement membrane (BM) collagens decreased and that of noncollagenous glycoproteins increased. This may change the structure of the BM in a less collagenous direction and affect its properties.
Lehti
T Maarit
TM
LIKES Research Center for Sport and Health Sciences, Rautpohjankatu 8, Viveca, FIN-40700 Jyväskylä, Finland. maarit.lehti@likes.fi
Silvennoinen
Mika
M
Kivelä
Riikka
R
Kainulainen
Heikki
H
Komulainen
Jyrki
J
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000502:physiology; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003094:Collagen / Q000235:genetics; D055513:Connective Tissue Growth Factor; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics; D017874:Immediate-Early Proteins / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011509:Proteoglycans / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics
D001786:Blood Glucose; C526318:CCN2 protein, mouse; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; D055513:Connective Tissue Growth Factor; D003094:Collagen; D002950:Citrate (si)-Synthase