Degradation of dehydrodivanillin by anaerobic bacteria from cow rumen fluid.
Dehydrodivanillin (DDV; 0.15 g/liter) was biodegradable at 37 degrees C under strictly anaerobic conditions by microflora from cow rumen fluid to the extent of 25% within 2 days in a yeast extract medium. The anaerobes were acclimated on DDV for 2 weeks, leading to DDV-degrading microflora with rates of degradation eight times higher than those initially. Dehydrodivanillic acid and vanillic acid were detected in an ethylacetate extract of a DDV-enriched culture broth by thin-layer, gas, and high-performance liquid chromatographies and by mass spectrometry.
Chen
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Department of Food Science and Technology and Department of Forest Products, School of Agriculture, Nagoya University, Chikusa, Nagoya 464, Japan.
Ohmiya
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Shimizu
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Kawakami
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