Differential gene expression profiling between wild-type and ALAS2-null erythroblasts: identification of novel heme-regulated genes.
To identify erythroid-specific heme-regulated genes, we performed differential expression analysis between wild-type and heme-deficient erythroblasts, which had been prepared from wild-type and erythroid-specific delta-aminolevulinate synthase-null mouse ES cells, respectively. Among 8737 clones on cDNA array, 40 cDNA clones, including 34 unknown ESTs, were first selected by their high expression profiles in wild-type erythroblasts, and evaluated further for their erythroid-lineage specificity, expression in hematopoietic tissues in vivo, and heme-dependent expression, which yielded 11, 4, and 4 genes, respectively. Because of the selection strategy employed, the final 4 were considered as the newly identified erythroid-specific heme-regulated genes. These 4 genes were uncoupling protein 2, nucleolar spindle-associated protein, cellular nucleic acid-binding protein, and a novel acetyltransferase-like protein. These findings thus suggest that heme may regulate a wide variety of hitherto unrecognized genes, and further analysis of these genes may clarify their role in erythroid cell differentiation.
Fujiwara
Tohru
T
Department of Rheumatology and Hematology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Harigae
Hideo
H
Takahashi
Shinichiro
S
Furuyama
Kazumichi
K
Nakajima
Osamu
O
Sun
Jiying
J
Igarashi
Kazuhiko
K
Yamamoto
Masayuki
M
Sassa
Shigeru
S
Kaku
Mitsuo
M
Sasaki
Takeshi
T
D000624:5-Aminolevulinate Synthetase / Q000172:deficiency / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004900:Erythroblasts / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006418:Heme / Q000378:metabolism*; D051379:Mice
D006418:Heme; D000624:5-Aminolevulinate Synthetase; C480079:ALAS2 protein, human