title: Heat shock protein 60: identification of specific epitopes for binding to primary macrophages.
abstract: In the present study, we characterized regions of human heat shock protein (HSP) 60 responsible for binding to primary macrophages. Studies using 20-mer peptides of the HSP60 sequence to compete with HSP60-binding to macrophages from C57BL/6J mice showed that regions aa241-260, aa391-410 and aa461-480 are involved in surface-binding. HSP60 mutants, lacking the N-terminal 137, 243 or 359 amino acids, inhibited HSP60-binding to primary macrophages to different degrees, demonstrating that all three regions are required for optimal binding. Analysis of different pro- and eukaryotic HSP60 species indicated that phylogenetically separate HSP60 species use different binding sites on primary macrophages.
authors.affiliation: German Diabetes Clinic, German Diabetes Center, Leibniz Institute, Heinrich-Heine-University of Düsseldorf, D-40225 Düsseldorf, Germany. christiane.habich@ddz.uni-duesseldorf.de
authors.forename: Christiane
authors.initials: C
authors.lastname: Habich
authors.forename: Karina
authors.initials: K
authors.lastname: Kempe
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Gomez
authors.forename: Mark
authors.initials: M
authors.lastname: Lillicrap
authors.forename: Hill
authors.initials: H
authors.lastname: Gaston
authors.forename: Ruurd
authors.initials: R
authors.lastname: van der Zee
authors.forename: Hubert
authors.initials: H
authors.lastname: Kolb
authors.forename: Volker
authors.initials: V
authors.lastname: Burkart
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D018834:Chaperonin 60 / Q000276:immunology*; D000939:Epitopes / Q000276:immunology* / Q000378:metabolism; D006658:Histoplasma / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D011485:Protein Binding / Q000276:immunology; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D000939:Epitopes