Araguari virus, a new member of the family Orthomyxoviridae: serologic, ultrastructural, and molecular characterization.
This paper reports the results of serologic, structural, biochemical, and genetic studies indicating that Araguari virus, a previously unassigned viral agent, is a member of the family Orthomyxoviridae and genus Thogotovirus. Araguari virus has six RNA fragments; biologically, it shares several properties with other viruses in the family Orthomyxoviridae. Nucleotide sequencing of the RNA segments 4 (glycoprotein) and 5 (nucleoprotein) of Araguari virus aligned with the orthomyxoviruses, showing the closest relationship with Thogoto virus (sequence similarity = 61.9% and 69.1%, respectively, for glycoprotein and nucleoprotein), but also sharing a more distant similarity with Dhori and Influenza C viruses, especially for the glycoprotein gene. Based on these results, we propose that Araguari virus should be assigned as a new member of the family Orthomyxoviridae and genus Thogotovirus.
Da Silva
Eliana V Pinto
EV
Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, Belém, Pará, Brazil.
Da Rosa
Amelia P A Travassos
AP
Nunes
Márcio R T
MR
Diniz
José A P
JA
Tesh
Robert B
RB
Cruz
Ana C R
AC
Vieira
Conceição M A
CM
Vasconcelos
Pedro F C
PF
D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D003168:Complement Fixation Tests; D018076:DNA, Complementary / Q000032:analysis; D006385:Hemagglutination Inhibition Tests; D008394:Marsupialia / Q000821:virology; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009975:Orthomyxoviridae / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D012367:RNA, Viral / Q000032:analysis; D016415:Sequence Alignment; D014709:Vero Cells
D018076:DNA, Complementary; D012367:RNA, Viral