Identification of lotus rhizobia by direct DNA hybridization of crushed root nodules.
Hybridization of crushed Lotus pedunculatus root nodules with P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. (Lotus rhizobia). Probes always hybridized with homologous target DNA and frequently with DNAs of other strains from the same genus. Intergeneric hybridization did not occur. Results were comparable to those from colony hybridization.
Cooper
J E
JE
Agricultural and Food Bacteriology Research Division, Department of Agriculture for Northern Ireland, Belfast BT9 5PX, Northern Ireland.
Bjourson
A J
AJ
Thompson
J K
JK