title: Retention behavior of silica-bonded and novel polymeric reversed-phase sorbents in studies on flavones as chemotaxonomic markers of Scutellaria L. genus.
abstract: In this study, the effectiveness of both classical and novel polymeric sorbents used in solid-phase extraction (SPE) for isolation of pharmacologically active flavones (baicalin, luteolin, apigenin, wogonin and chrysin) from aerial parts of 13 species of Scutellaria L. (Skullcap) genus was assessed. The highest recoveries of hydrophilic (baicalin, luteolin) flavones for Oasis HLB were obtained, whereas for medium hydrophobic (apigenin) and hydrophobic (wogonin, chrysin) flavones better quantitative results for BakerBond phenyl cartridges were stated. Eluates were analysed using reversed-phase high-performance liquid chromatography with diode array detection (RP-HPLC-DAD). For the five target compounds the determined concentrations ranged from approximately 4 to approximately 15,500 microg/g dry wt. Very good linearities (r(2)>0.9995) of calibration curves were achieved for each flavone. The accuracy was below 5% for most compounds examined. This is the first method reported that enabled simultaneous qualification and quantitation of five flavones (being chemotaxonomic markers) in 13 species of Scutellaria L. genus.
authors.affiliation: Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University of Lublin, 1 Chodźki Street, 20-093 Lublin, Poland. gzgorka@pharmacognosy.org
authors.forename: Grazyna
authors.initials: G
authors.lastname: Zgórka
mesh_terms: D047310:Apigenin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D002630:Chemotactic Factors / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D047309:Flavones / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D047311:Luteolin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry; D031363:Scutellaria / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D002630:Chemotactic Factors; D047309:Flavones; D005419:Flavonoids; D010936:Plant Extracts; D011108:Polymers; C038044:baicalin; C043561:chrysin; D012822:Silicon Dioxide; D047310:Apigenin; D047311:Luteolin