Approach to novel functional foods for stress control 2. Microarray assessment of exercise in healthy volunteers.
DNA microarray was used to measure stress response by exercise in peripheral blood leukocytes. Aerobic exercise did not alter mRNA pattern or urinary secretion of 8-hydroxy-2'-deoxyguanosine (8-OHdG). Strenuous exercise increased urinary secretion of 8-OHdG and altered mRNA pattern in microarray. These results suggest that moderate exercise, i. e. aerobic exercise, did not show any change in 8-OHdG, an oxidative stress marker, or mRNA expression in the leukocytes, which might reflect whole body neurohormanal changes. In addition, strenuous exercise produced quite different expression pattern from those of psychological stress.
Nakaya
Yutaka
Y
Department of Nutrition, The University of Tokushima Graduate School, Japan.
Hata
Yasuko
Y
Ishida
Kaori
K
Takahashi
Akira
A
Morita
Kyoko
K
Rokutan
Kazuhito
K
D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000652:urine; D004247:DNA / Q000032:analysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D015444:Exercise; D005502:Food; D006304:Health Status; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010807:Physical Endurance; D005082:Physical Exertion; D012333:RNA, Messenger / Q000378:metabolism; D013315:Stress, Psychological / Q000517:prevention & control*; D013997:Time Factors; D014838:Volunteers
D015415:Biomarkers; D012333:RNA, Messenger; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine