title: Natural killer cell activities of synbiotic Lactobacillus casei ssp. casei in conjunction with dextran.
abstract: We have reported previously that Lactobacillus casei ssp. casei, together with specific substrate dextran, exhibited an adjuvant effect of stimulating humoral immune responses against bovine serum albumin (BSA) as a model antigen in BALB/c mice. In the present study, among the Lactobacillus species tested, L. casei ssp. casei with dextran significantly elevated the natural killer (NK) cell activities in spleen mononuclear cells from BALB/c mice in comparison to L. casei ssp. casei alone or other Lactobacillus species with or without dextran. Oral administration of L. casei ssp. casei together with dextran also resulted in a significant increase of NK cell activities in healthy human volunteers. Further, L. casei ssp. casei induced significant production of interleukin (IL)-12 in human peripheral blood mononuclear cells and IL-15 mRNA expression in the human intestinal epithelial cell line Caco-2. L. casei ssp. casei with dextran in food also significantly elevated the survival rate of BALB/c mice bearing Meth-A cells. Taken together, these results demonstrate that dietary synbiotic supplementation which is a combination of the L. casei ssp. casei used as a probiotic together with the dextran, a specific substrate as a prebiotic, efficiently elicits murine and human NK cell activities.
authors.affiliation: Department of Oral Microbiology, Asahi University School of Dentistry, Gifu, Japan. tomo527@dent.asahi-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: Y
authors.initials: Y
authors.lastname: Asai
authors.forename: R
authors.initials: R
authors.lastname: Tamai
authors.forename: Y
authors.initials: Y
authors.lastname: Makimura
authors.forename: H
authors.initials: H
authors.lastname: Sakamoto
authors.forename: S
authors.initials: S
authors.lastname: Hashikawa
authors.forename: K
authors.initials: K
authors.lastname: Yasuda
mesh_terms: D000328:Adult; D000818:Animals; D000917:Antibody Formation; D002460:Cell Line; D003911:Dextrans / Q000008:administration & dosage*; D019587:Dietary Supplements; D005260:Female; D006801:Humans; D018664:Interleukin-12 / Q000032:analysis; D019409:Interleukin-15 / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology*; D007780:Lacticaseibacillus casei / Q000276:immunology* / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D019936:Probiotics; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012710:Serum Albumin, Bovine / Q000008:administration & dosage
chemical_list: D003911:Dextrans; D019409:Interleukin-15; D012333:RNA, Messenger; D018664:Interleukin-12; D012710:Serum Albumin, Bovine