Improved cell dispersal method by low-temperature incubation of tissue in a trypsin-citrate solution.
Tissues soaked at low temperature in a trypsin-citrate solution, washed to remove enzymes, and disrupted by rapid mechanical vibrations gave yields of viable cells 1.6 to 5 times those from conventional procedures.
van Wezel
A L
AL
Rijks Instituut voor de Volksgezondheid, Postbus 1, Bilthoven, The Netherlands.
van Hemert
P A
PA
Parisius
W
W
Cucakovich
N B
NB
Macmorine
H G
HG