Negative feedback loop formed by Lunatic fringe and Hes7 controls their oscillatory expression during somitogenesis.
Recent studies show that the cyclic expression of Lfng in the presomitic mesoderm (PSM) is controlled at the transcription level by Notch signal through the CBF1 binding site for activation and periodic repression on the Lfng promoter. Here we provide genetic evidence that the oscillatory expression is controlled by a negative feedback mechanism. We also show that Hes7, another cyclically expressed protein, can bind to the N-boxes on both Lfng and its own promoters and repress their activity. In addition, we demonstrate that the 3' untranslated region (3'-UTR) is important for rapid degradation of Lfng mRNA.
Chen
Jun
J
Laboratory of Developmental Genetics, Van Andel Research Institute, Grand Rapids, Michigan 49503, USA.
Kang
Liang
L
Zhang
Nian
N
D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004622:Embryo, Mammalian / Q000378:metabolism; D025461:Feedback, Physiological; D018507:Gene Expression Regulation, Developmental; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009024:Morphogenesis; D011401:Promoter Regions, Genetic; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D019170:Somites / Q000502:physiology*
D020413:3' Untranslated Regions; D051792:Basic Helix-Loop-Helix Proteins; D016695:Glycosyltransferases; D012333:RNA, Messenger; D051880:Receptors, Notch; C430525:Hes7 protein, mouse; C498392:Lfng protein, mouse