title: TGF-beta1 favors the development of fast type identity during soleus muscle regeneration.
abstract: Transforming growth factor-beta1 (TGF-beta1) is known to be expressed in the environment of developing fast muscle fibres during ontogenesis. In the present study, we have examined effects of administration of either TGF-beta1 or neutralizing TGF-beta1 antibody on the induction of fast type phenotype in regenerating skeletal muscles in rats. Expressions of fast and slow myosin heavy chain (MHC) isoforms were studied using protein electrophoresis, at 3 and 6 weeks after myotoxic treatment. Muscle contractile properties were also measured in situ. The results have shown that a single injection of TGF-beta1 into the regenerating slow soleus muscle increased the expression of fast MHC-2x/d and MHC-2a and decreases that of slow MHC-1 (P<0.05). Moreover, it reduced the degree of tetanic fusion during contraction (P<0.05). Conversely, injection of neutralizing antibody against TGF-beta1 into the regenerating fast EDL muscle increased the expression of MHC-2a and MHC-1 (P<0.05). In conclusion, when the slow muscle was regenerating in the presence of an increased level of TGF-beta1, it induced a shift to a less slow MHC phenotype and contractile characteristics. Conversely, neutralization of TGF-beta1 in the regenerating fast muscle induced a shift to a less fast MHC expression. Together these results suggest that TGF-beta1 influences some aspects of fast muscle-type patterning during skeletal muscle regeneration.
authors.affiliation: CNRS FRE 2412, Créteil, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Noirez
authors.forename: Sandra
authors.initials: S
authors.lastname: Torres
authors.forename: José
authors.initials: J
authors.lastname: Cebrian
authors.forename: Onnik
authors.initials: O
authors.lastname: Agbulut
authors.forename: Juliette
authors.initials: J
authors.lastname: Peltzer
authors.forename: Gillian
authors.initials: G
authors.lastname: Butler-Browne
authors.forename: Dominique
authors.initials: D
authors.lastname: Daegelen
authors.forename: Isabelle
authors.initials: I
authors.lastname: Martelly
authors.forename: Angelica
authors.initials: A
authors.lastname: Keller
authors.forename: Arnaud
authors.initials: A
authors.lastname: Ferry
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D018656:Muscle Fibers, Fast-Twitch / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000187:drug effects / Q000378:metabolism; D010641:Phenotype; D020033:Protein Isoforms / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D032496:Satellite Cells, Skeletal Muscle / Q000187:drug effects / Q000378:metabolism; D014118:Toxins, Biological / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
chemical_list: D000906:Antibodies; D020033:Protein Isoforms; D014118:Toxins, Biological; D053773:Transforming Growth Factor beta1; D018995:Myosin Heavy Chains