title: No delayed temporal response to sample concentration changes during enhanced microdialysis sampling using cyclodextrins and antibody-immobilized microspheres.
abstract: The temporal response to concentration changes external to a microdialysis probe containing trapping agents in the perfusion fluid was studied. Native beta-cyclodextrin and a water-soluble beta-cyclodextrin polymer were used as trapping agents in the microdialysis perfusion fluid to study the temporal concentration response to carbamazepine, a hydrophobic analyte. The temporal response of microdialysis probes containing antibody-immobilized microspheres against five different cytokines (tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-2 (IL-2), IL-4, and IL-5) to concentration changes outside of the probe was also determined. In both cases, no delayed temporal response of enhanced microdialysis was observed for either carbamazepine or the cytokines as compared to standard microdialysis sampling procedures.
authors.affiliation: Department of Chemistry and Chemical Biology and Center for Biotechnology and Interdisciplinary Studies, Cogswell Laboratories, Rensselaer Polytechnic Institute, 110 Eighth Street, Troy, NY 12180-3590, USA.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ao
authors.forename: Julie A
authors.initials: JA
authors.lastname: Stenken
mesh_terms: D000818:Animals; D002220:Carbamazepine / Q000032:analysis* / Q000493:pharmacokinetics; D016207:Cytokines / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D017551:Microdialysis / Q000379:methods*; D008863:Microspheres; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D016207:Cytokines; D047392:beta-Cyclodextrins; D002220:Carbamazepine; C031215:betadex