title: Optimization of the Sistrom Culture Medium for Large-Scale Batch Cultivation of Rhodospirillum rubrum under Semiaerobic Conditions with Maximal Yield of Photosynthetic Membranes.
abstract: The defined medium A of W. R. Sistrom (W. R. Sistrom, J. Gen. Microbiol. 22:77-85, 1960) has been modified to allow the growth of Rhodospirillum rubrum in large-scale batch cultures under dark, semiaerobic conditions. The simultaneous use of two substrates, NH(4)-succinate (46 mM) and fructose (0.3%), which are utilized in aerobic and fermentative metabolism, respectively, leads to very high cell densities with a maximal yield of photosynthetic membranes.
authors.affiliation: Department of Microbiology, Biocenter, CH-4056 Basel, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Ghosh
authors.forename: A
authors.initials: A
authors.lastname: Hardmeyer
authors.forename: I
authors.initials: I
authors.lastname: Thoenen
authors.forename: R
authors.initials: R
authors.lastname: Bachofen