On-line microdevice for stress proteomics.
The handling of the cells or tissues is essential for proteomics research or drug screening, where labor is not avoidable. The steps of cell wash, protein extraction, protein denaturing are complicated procedures in conventional method using centrifugation and pipetting in the laboratory. This is the bottle-neck for proteome research. To solve these problems, we propose to utilize the nanotechnology, which will improve the proteomics methodology. Utilizing the nanotechnology, we developed a novel microseparation system, where centrifugation and pipetting are needless. This system has a nanostructured microdevice, by which the cell handling, protein extraction, and antibody assay can be performed. Since cell transfer is needless, all cells are corrected without any loss during the cell-pretreatment procedures, which allowed high reproducibility and enabled the detection of low amount of protein expression. Utilizing the microdevice, we analyzed the stress induced proteins. We further succeeded the screening of food that was useful for immunity and found that an extraction from seaweed promoted the apoptosis of T-lymphoblastic cells. Here, we present an on-line microdevice for stress proteomics.
Tabuchi
Mari
M
Department of Molecular and Pharmaceutical Biotechnology, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan.
Nagata
Hideya
H
Nomura
Masanori
M
Katsube
Sawako
S
Sawada
Akihiro
A
Joko
Akira
A
Hatta
Koji
K
Hagiwara
Naoto
N
Kobayashi
Kazuyoshi
K
Tomita
Fumiyo
F
Miki
Satoko
S
Arai
Katsuhiko
K
Ishiguro
Takashi
T
Baba
Yoshinobu
Y
D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D009862:Online Systems; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
D020543:Proteome