Plate assay for simultaneous detection of alginate lyases and determination of substrate specificity.
A plate assay to detect the presence of alginate lyases (EC 4.2.2.3) has been developed. The simultaneous use of specific alginate block structures of defined composition allows the substrate specificity of the enzymes to be determined. Clearing zones in the alginate-containing media are visualized with either cetyl pyridinium chloride or ruthenium red.
Gacesa
P
P
Department of Biochemistry, University of Wales, P.O. Box 903, Cardiff CF1 1ST, United Kingdom.
Wusteman
F S
FS