Hydrostatic pressure increases apoptosis in cartilage-constructs produced from human osteoarthritic chondrocytes.
Tissue-engineering is considered a promising avenue for developing human articular cartilage implants that can be employed for resurfacing damaged cartilage in the early stages of osteoarthritis. In the present study, human cartilage-constructs were produced from human osteoarthritic chondrocytes maintained on a scaffold of HYAFFR-11 in perfusion mini-bioreactors or after implantation and recovery from nude or SCID mice after 3 weeks. The human cartilage-construct extracellular matrix reacted positively with anti-Type II collagen monoclonal antibody, but not with anti-Type I or anti-Type X collagen monoclonal antibodies. A significant portion of the cartilage-construct extracellular matrix stained metachromatic with Toluidine blue-O indicative of sulfated-proteoglycan deposition. Cyclic hydrostatic pressure applied for 4 hrs at 5 MPa using a 1 Hertz sinusoidal frequency significantly increased (p < 0.02) the proportion of apoptotic cells in the cartilage-constructs (41% +/- 4.2%; mean +/- SD) compared to control cartilage-constructs (28.5 +/- 8.4%).
Wenger
Reid
R
Department of Orthodontics, Case Western Reserve University School of Dentistry, Cleveland, Ohio 44106-4946, USA.
Hans
Mark G
MG
Welter
Jean F
JF
Solchaga
Luis A
LA
Sheu
Yun R
YR
Malemud
Charles J
CJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D017209:Apoptosis; D019149:Bioreactors; D002356:Cartilage / Q000378:metabolism / Q000473:pathology*; D002358:Cartilage, Articular / Q000378:metabolism; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D006874:Hydrostatic Pressure; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D010003:Osteoarthritis / Q000378:metabolism / Q000473:pathology*; D011509:Proteoglycans / Q000737:chemistry; D023822:Tissue Engineering; D014048:Tolonium Chloride / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D004277:DNA, Single-Stranded; D011509:Proteoglycans; D014048:Tolonium Chloride; D003094:Collagen