Production of enterotoxin a.
A method for production of enterotoxin A in multiple liter lots is described. The medium contained 4% N-Z Amine NAK supplemented with 0.001% niacin and 0.00005% thiamine, and was adjusted to pH 6. The inoculated medium in lots of 400 to 600 ml, in 2-liter Erlenmeyer flasks, was incubated at 37 C for 24 hr on a gyrotory shaker at 280 rev/min. Production of 4 to 6 mug of enterotoxin A per ml occurred.
Kato
E
E
Food Research Institute and Department of Biochemistry, University of Chicago, Chicago, Illinois.
Khan
M
M
Kujovich
L
L
Bergdoll
M S
MS