Molecular Cloning of the Tryptophan Operon from an Aeromonas hydrophila Freshwater Isolate.
A genomic library of Aeromonas hydrophila F9 was constructed by using pBR322 as a vector. From that, two DNA fragments (5.8 and 11.6 kb) were isolated containing genetic information to complement trpA and trpB defects (5.8-kb fragment) and to complement trpA, trpB, trpC, trpD, and trpE defects (11.6-kb fragment) in Escherichia coli mutants. Evidence of the existence of a secondary promoter is given.
Anguita
J
J
Departamento de Patología Animal (Sanidad Animal), Unidad de Microbiología, Facultad de Veterinaria, Universidad de León, 24071 León, Spain.
Rivero
O
O
Naharro
G
G