Expression of urokinase-type plasminogen activator receptor is correlated with metastases of lingual squamous cell carcinoma.
Lingual squamous cell carcinoma is common and the survival rate is relatively low. The invasion of cancer cells from the primary tumour into the surrounding tissue is an early step in the process of metastasis and urokinase-type plasminogen activator receptor (uPAR) is a vital mediator of cellular migration in some carcinomas. By binding urokinase-type plasminogen activator, uPAR localises proteolytic activity to the leading edge of the cells, thereby facilitating cellular migration and penetration through tissue boundaries. uPAR also binds directly to vitronectin and associates with integrins within the plasma membrane, which alters the strength of cellular adhesion. In this study we used reverse transcription polymerase chain reaction, immunocytochemistry, and Western-blot to examine the expression of uPAR mRNA and protein in Ts and Tca 8113 cell lines of lingual squamous cell carcinoma and in normal oral mucosal cells. uPAR mRNA and protein were expressed in Ts cells, but not in Tca 8113 cells or in normal oral epithelial cells. Ts cells have higher metastatic potential than Tca 8113 cells. The results suggest that uPAR has an important role in the aggressiveness of lingual squamous cell carcinoma.
Wang
Jing
J
Department of Oral Biology, College of Stomatology, Fourth Military Medical University, Xi'an Shannxi 710032, China. wangjing1971@163.com
Guo
Fuping
F
Wei
Hongyu
H
Dong
Jijun
J
Wu
Junzheng
J
D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000473:pathology / Q000556:secondary*; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000201:enzymology; D002465:Cell Movement / Q000502:physiology; D004792:Enzyme Precursors / Q000032:analysis*; D004847:Epithelial Cells / Q000201:enzymology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000378:metabolism; D009061:Mouth Mucosa / Q000201:enzymology / Q000473:pathology; D009361:Neoplasm Invasiveness; D011485:Protein Binding / Q000502:physiology; D011956:Receptors, Cell Surface / Q000032:analysis*; D055293:Receptors, Urokinase Plasminogen Activator; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014062:Tongue Neoplasms / Q000201:enzymology* / Q000473:pathology; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis*; D019096:Vitronectin / Q000378:metabolism
D004792:Enzyme Precursors; D016023:Integrins; C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D019096:Vitronectin; D014568:Urokinase-Type Plasminogen Activator