[Plaferon LB as a modulator of the activity of transport protein complexes of mitochondrial respiratory chain].
Mitochondrial energogenesis plays key role in the vital activity of cells. Regulation of its intensity is especially important for the maintenance of function of tissues and body internal organs under different pathological conditions. There are numerous studies demonstrating modulator effect of domestic preparation, plaferon LB (USA patent number WO02/ 12444 A2) on the intensity of mitochondrial respiration in vivo at different pathological processes. In order to determine the mechanism of modulator effect of plaferon LB we have studied effectiveness of its effect on the intensity of electron transport in mitochondrial respiratory chain, inhibited by different toxins, in isolated myocardial sections in vitro. Isolated sections (100mg) of myocardium of white rats were placed in the buffer solution with glutamate and DNF. Obtained dredge was distributed in equal portions to the different cells, where amital -- inhibitor of I complex of mitochondrial electron transport chain or potassium cyanide, inhibitor of IV complex of mitochondrial electron transport chain, or plaferon LB have been added in different consecution with 2 min intervals (USA patent number WO02/ 12444 A2). We discovered that plaferon LB rehabilitates electron transport on the sites of I and IV complexes of mitochondrial chain that leads to a decrease in free radical production and normalization of cell redox status. This is the mechanism through which the preparation regulates the intensity of mitochondrial respiration and tissues energogenesis.
Nasrashvili
M G
MG
Datunashvili
I V
IV
Shushtakashvili
E I
EI
Papava
M B
MB
D002352:Carrier Proteins / Q000187:drug effects*; D004579:Electron Transport / Q000187:drug effects*; D006801:Humans; D008297:Male; D008931:Mitochondria, Muscle / Q000187:drug effects* / Q000378:metabolism*; D009479:Neuropeptides / Q000008:administration & dosage / Q000494:pharmacology*
D002352:Carrier Proteins; D009479:Neuropeptides; C091912:plaferon