title: Selective inhibition by 2-bromoethanesulfonate of methanogenesis from acetate in a thermophilic anaerobic digestor.
abstract: The effects of 2-bromoethanesulfonate, an inhibitor of methanogenesis, on metabolism in sludge from a thermophilic (58 degrees C) anaerobic digestor were studied. It was found from short-term experiments that 1 mumol of 2-bromoethanesulfonate per ml completely inhibited methanogenesis from CH(3)COO, whereas 50 mumol/ml was required for complete inhibition of CO(2) reduction. When 1 mumol of 2-bromoethanesulfonate per ml was added to actively metabolizing sludge which was then incubated for 24 h. it caused a 60% reduction in methanogenesis and a corresponding increase in acetate accumulation; at 50 mumol/ml it caused complete inhibition of methanogenesis and accumulation of acetate. H(2), and ethanol.
authors.affiliation: Department of Microbiology, Stocking Hall, Cornell University, Ithaca, New York 14853.
authors.forename: S H
authors.initials: SH
authors.lastname: Zinder
authors.forename: T
authors.initials: T
authors.lastname: Anguish
authors.forename: S C
authors.initials: SC
authors.lastname: Cardwell