A system for the detection and typing of human papillomavirus of the lower genital tract: in situ hybridization screening and polymerase chain reaction confirmation.
We developed a simplified and non-isotopic in situ hybridization procedure for the detection of Human Papillomavirus (HPV) in routine Papanicolaou cervical smears. The assay involves one oligonucleotide (malignant probe) which recognizes high risk HPV 16 and 18, and another which detects HPV 6 and 11 (benign probe). We adapted the system to fulfill the requirements of gynecologists and cytologists, assimilating their protocols and simplifying the in situ hybridization assay. When we compared the detection levels reached by the in situ hybridization versus a ladder PCR assay in 156 clinical samples, original designed for this work, the kappa coefficient between both methods is 0.945, indicating a strong agreement between the ISH and the PCR assays.
Premoli
Gloria
G
Centro de Investigaciones Odontológicas, Facultad de Odontología, Edificio el Rectorado, Universidad de Los Andes, Apartado postal 64. Calle 23 entre Av. 2 y 3, La Hechicera, Mérida 5101, Venezuela. premoli@ula.ve
Galindo-Castro
Ivan
I
Ramírez
José Luis
JL
D002584:Cervix Uteri / Q000821:virology*; D015344:DNA Probes, HPV; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000145:classification* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014626:Vaginal Smears
D015344:DNA Probes, HPV