Extremely rapid extraction of DNA from bacteria and yeasts.
A very simple and rapid method for extracting genomic DNA from Gram-negative bacteria, Gram-positive bacteria and yeasts is presented. In this method, bacteria or yeasts are lysed directly by phenol and the supernatant is extracted with chloroform to remove traces of phenol. The supernatant contains DNA that is suitable for molecular analyses, such as PCR, restriction enzyme digestion and genomic library construction. This method is reproducible and simple for the routine DNA extraction from bacteria and yeasts.
Cheng
Hai-Rong
HR
Center for Microbial Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, China.
Jiang
Ning
N
D002725:Chloroform / Q000494:pharmacology; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000302:isolation & purification*; D006090:Gram-Negative Bacteria / Q000235:genetics*; D006094:Gram-Positive Bacteria / Q000235:genetics*; D019800:Phenol / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004269:DNA, Bacterial; D004271:DNA, Fungal; D019800:Phenol; D002725:Chloroform