Rosiglitazone protects human neuroblastoma SH-SY5Y cells against acetaldehyde-induced cytotoxicity.
Acetaldehyde, an inhibitor of mitochondrial function, has been widely used as a neurotoxin because it elicits a severe Parkinson's disease-like syndrome with elevation of the intracellular reactive oxygen species level and apoptosis. Rosiglitazone, a peroxisome proliferator-activated receptor-gamma agonist, has been known to show various non-hypoglycemic effects, including anti-inflammatory, anti-atherogenic, and anti-apoptotic. In this study, we investigated the protective effects of rosiglitazone on acetaldehyde-induced apoptosis in human neuroblastoma SH-SY5Y cells and attempted to examine its mechanism. Acetaldehyde-induced apoptosis was moderately reversed by rosiglitazone treatment. Our results suggest that the protective effects of rosiglitazone on acetaldehyde-induced apoptosis may be ascribed to ability to induce the expression of anti-oxidant enzymes and to regulate Bcl-2 and Bax expression. These data indicate that rosiglitazone may provide a useful therapeutic strategy for the prevention of progressive neurodegenerative disease such as Parkinson's disease.
Jung
Tae Woo
TW
The Brain Korea 21 Project for Medical Science, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea.
Lee
Ji Young
JY
Shim
Wan Sub
WS
Kang
Eun Seok
ES
Kim
Soo Kyung
SK
Ahn
Chul Woo
CW
Lee
Hyun Chul
HC
Cha
Bong Soo
BS
D000079:Acetaldehyde / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology* / Q000503:physiopathology*; D018696:Neuroprotective Agents / Q000008:administration & dosage; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000008:administration & dosage*
D004338:Drug Combinations; D018696:Neuroprotective Agents; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D000079:Acetaldehyde