RT-PCR determination of maspin and mammaglobin B in peripheral blood of healthy donors and breast cancer patients.
BACKGROUND
The aim of the present study was to evaluate the accuracy of two markers, maspin and mammaglobin B, singly or in combination, to detect breast cancer. To define better the potential and limits of the two markers for diagnostic purposes, blood positivity was analyzed in relation to clinical, pathological and biological tumor characteristics.


METHODS
The markers were determined in peripheral blood (PB) samples from 27 healthy donors and 140 previously untreated patients using nested reverse transcriptase polymerase chain reaction (RT-PCR).


RESULTS
Positivity for maspin in blood samples was observed in 24% of patients with an 89% specificity. For mammaglobin B, positivity was observed in 7% of patients and never in healthy donors. The presence of maspin was correlated with cell proliferation of the primary tumor (P = 0.015), whereas mammaglobin B positivity correlated with pathological stage (P = 0.013). The presence of either marker was significantly related to nodal status.


CONCLUSIONS
Our results indicate that the two markers in association could represent a potentially useful non-invasive tool to detect breast cancer. The validation of these markers as indicators of high risk of relapse is ongoing in a series of patients with an adequate follow-up.
Mercatali
L
L
Istituto Oncologico Romagnolo, Forlì, Italy.
Valenti
V
V
Calistri
D
D
Calpona
S
S
Rosti
G
G
Folli
S
S
Gaudio
M
M
Frassineti
G L
GL
Amadori
D
D
Flamini
E
E
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000097:blood; D001943:Breast Neoplasms / Q000097:blood*; D045744:Cell Line, Tumor; D017931:DNA Primers; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D060788:Mammaglobin B; D008875:Middle Aged; D009185:Myelin Proteins; D009363:Neoplasm Proteins / Q000097:blood*; D011510:Proteolipids; D020133:Reverse Transcriptase Polymerase Chain Reaction; D060765:Secretoglobins; D012680:Sensitivity and Specificity; D015843:Serpins / Q000097:blood*; D014598:Uteroglobin / Q000097:blood*
D014408:Biomarkers, Tumor; D017931:DNA Primers; D060788:Mammaglobin B; D009185:Myelin Proteins; D009363:Neoplasm Proteins; D011510:Proteolipids; C556467:SCGB2A1 protein, human; C085344:SERPIN-B5; D060765:Secretoglobins; D015843:Serpins; D014598:Uteroglobin