title: Kinetics of phenol biodegradation by an immobilized methanogenic consortium.
abstract: A phenol-degrading methanogenic enrichment was successfully immobilized in agar as shown by the stoichiometric conversion of phenol to CH(4) and CO(2). The enrichment contained members of three physiological groups necessary for the syntrophic mineralization of phenol: a phenol-oxidizing bacterium, a Methanothrix-like bacterium, and an H(2)-utilizing methanogen. The immobilization technique resulted in the cells being embedded in a long, thin agar strand (1 mm in diameter by 2 to 50 cm in length) that resembled spaghetti. Immobilization had three effects as shown by a comparative kinetic analysis of phenol degradation by free versus immobilized cells. (i) The maximum rate of degradation was reduced from 14.8 to 10.0 mug of phenol per h; (ii) the apparent K(m) for the overall reaction was reduced from 90 to 46 mug of phenol per ml, probably because of the retention of acetate, H(2) and CO(2) in the proximity of immobilized methanogens; and (iii) the cells were protected from substrate inhibition caused by high concentrations of phenol, which increased the apparent K(i) value from 900 to 1,725 mug of phenol per ml. Estimates for the kinetic parameters K(m), K(i), and V(max) were used in a modified substrate inhibition model that simulated rates of phenol degradation for given phenol concentrations. The simulated rates were in close agreement with experimentally derived rates for both stimulatory and inhibitory concentrations of phenol.
authors.affiliation: Department of Microbiology and Public Health and Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824.
authors.forename: D F
authors.initials: DF
authors.lastname: Dwyer
authors.forename: M L
authors.initials: ML
authors.lastname: Krumme
authors.forename: S A
authors.initials: SA
authors.lastname: Boyd
authors.forename: J M
authors.initials: JM
authors.lastname: Tiedje