title: Synthesis of 1,2-Epoxyoctane by Pseudomonas oleovorans During Growth in a Two-Phase System Containing High Concentrations of 1-Octene.
abstract: We have optimized and compared the synthesis of 1,2-epoxyoctane from 1-octene by resting and by growing cells of Pseudomonas oleovorans. The net production of 1,2-epoxyoctane by resting cells never exceeded 0.6 mg/ml of suspension. In contrast, P. oleovorans produced much more epoxide when it was grown on high levels of 1-octene. To raise the total production of epoxide, the octene layer was repeatedly transferred to fresh, growing cultures of P. oleovorans. By using this approach, a maximum of 28 mg of epoxide was synthesized per ml of total culture, resulting in the accumulation of ca. 75 mg of epoxide per ml in the octene phase.
authors.affiliation: Biochemisch Laboratorium, The University, 9747 AG Groningen, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: de Smet
authors.forename: H
authors.initials: H
authors.lastname: Wynberg
authors.forename: B
authors.initials: B
authors.lastname: Witholt