title: New insights into potential functions for the protein 4.1 superfamily of proteins in kidney epithelium.
abstract: Members of the protein 4.1 family of adapter proteins are expressed in a broad panel of tissues including various epithelia where they likely play an important role in maintenance of cell architecture and polarity and in control of cell proliferation. We have recently characterized the structure and distribution of three members of the protein 4.1 family, 4.1B, 4.1R and 4.1N, in mouse kidney. We describe here binding partners for renal 4.1 proteins, identified through the screening of a rat kidney yeast two-hybrid system cDNA library. The identification of putative protein 4.1-based complexes enables us to envision potential functions for 4.1 proteins in kidney: organization of signaling complexes, response to osmotic stress, protein trafficking, and control of cell proliferation. We discuss the relevance of these protein 4.1-based interactions in kidney physio-pathology in the context of their previously identified functions in other cells and tissues. Specifically, we will focus on renal 4.1 protein interactions with beta amyloid precursor protein (beta-APP), 14-3-3 proteins, and the cell swelling-activated chloride channel pICln. We also discuss the functional relevance of another member of the protein 4.1 superfamily, ezrin, in kidney physio-pathology.
authors.affiliation: Life Sciences Division, Department of Genome Biology, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
authors.forename: Venice
authors.initials: V
authors.lastname: Calinisan
authors.forename: Dana
authors.initials: D
authors.lastname: Gravem
authors.forename: Ray Ping-Hsu
authors.initials: RP
authors.lastname: Chen
authors.forename: Sachi
authors.initials: S
authors.lastname: Brittin
authors.forename: Narla
authors.initials: N
authors.lastname: Mohandas
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Lecomte
authors.forename: Philippe
authors.initials: P
authors.lastname: Gascard
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000378:metabolism; D000818:Animals; D017487:Ankyrins / Q000737:chemistry; D001798:Blood Proteins / Q000502:physiology*; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D018450:Disease Progression; D004848:Epithelium / Q000378:metabolism*; D015723:Gene Library; D006801:Humans; D007668:Kidney / Q000196:embryology / Q000378:metabolism*; D008565:Membrane Proteins; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D005810:Multigene Family; D009399:Nephrons / Q000378:metabolism; D009995:Osmosis; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D000099149:Ezrin
chemical_list: D048948:14-3-3 Proteins; D016229:Amyloid beta-Peptides; D017487:Ankyrins; D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D000099149:Ezrin; C028841:erythrocyte membrane band 4.1 protein