[3D cellular models: a powerful access to cutaneous physiology and to innovative developments of cosmetic active compounds].
Two different anti-ageing cosmetic actives based respectively, on dermal compartment regeneration and on dermoepidermal reinforcement, have been developed using tissular engineering models. Conjointly use of different innovative three-dimensional models such as reconstructed dermis and skin built with human cells coming from variable aged donors allowed first, a better understanding of matrix modifications due to ageing and second, the screening of active ingredients highly targeted to reverse observed damages. The validity of such three-dimensional models has been then confirmed by in vivo studies on healthy volunteers.
André
V
V
Coletica, 32, rue Saint-Jean-de-Dieu, 69007 Lyon, France. vandre@coletica.fr
Grenier
S
S
Pivard
F
F
Perrier
E
E
D000328:Adult; D000367:Age Factors; D000368:Aged; D000826:Animal Testing Alternatives; D002448:Cell Adhesion; D048271:Chitosan; D002809:Chondroitin Sulfates; D024042:Collagen Type I; D024061:Collagen Type III; D003358:Cosmetics / Q000494:pharmacology; D003470:Culture Media; D004353:Drug Evaluation, Preclinical; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis; D005781:Gelatin Sponge, Absorbable; D006801:Humans; D007797:Laminin / Q000096:biosynthesis; D008875:Middle Aged; D012867:Skin / Q000648:ultrastructure; D012879:Skin Physiological Phenomena; D017768:Skin, Artificial; D046509:Tissue Culture Techniques / Q000295:instrumentation*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*
D024042:Collagen Type I; D024061:Collagen Type III; D003358:Cosmetics; D003470:Culture Media; D005353:Fibronectins; D007797:Laminin; D002809:Chondroitin Sulfates; D048271:Chitosan