Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells.
A randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production approximately 10-fold at approximately 60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells.
Kwon
Ryuk-Jun
RJ
ToolGen, Inc. Daejeon Bio-Venture Town, 461-58, Jeonmin-dong, 305-390, Yuseong-gu, Daejeon, South Korea.
Kim
Seong Keun
SK
Lee
Seung-Il
SI
Hwang
Su-Jeong
SJ
Lee
Gyun Min
GM
Kim
Jin-Soo
JS
Seol
Wongi
W
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D005822:Genetic Vectors; D006801:Humans; D019151:Peptide Library; D011994:Recombinant Proteins / Q000096:biosynthesis; D012190:Retroviridae / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic; D016335:Zinc Fingers / Q000235:genetics
D000911:Antibodies, Monoclonal; D019151:Peptide Library; D011994:Recombinant Proteins; D014157:Transcription Factors