[Telomerase: the expression and regulatory mechanisms in preovulatory ovarian granulosa cells].
The objective of this study was to analyze the expression of telomerase in granulosa cells and the influential factors in telomerase expression. TRAP-ELISA (telomeric repeat amplification protocol-enzyme linked immunoadsordent assay) method was used to study the expression and control of telomerase in rat preovulatory ovary. We also used radioimmunoassay (RIA) to determine the expression of estradiol (E(2)) and progesterone (P(0)). Furthermore we used MTT to study the proliferation of ovarian granulosa cells. Telomerase activity was significantly enhanced by human chorionic gonadotropin (HCG), follicle-stimulating hormone (FSH), verapamil and dbcAMP, and was significantly reduced by antisense-c-myb oligodeoxynucleotide (anti-c-myb ODN) treatment. RIA was used to determine the secretion of P(0) and E(2) in all these cell culture media. We found that the secretion of these two hormones was increased when verapamil and FSH were added; no change after adding HCG and dbcAMP; and reduced when anti-c-myb was added. In MTT assay, we found that the antisense hTERT ODN significantly inhibited the proliferation of ovarian granulosa cells. These results demonstrate that telomerase activity is present in ovary antral granulosa cells and its activity is controlled by FSH, HCG, verapamil and anti-c-myb, and is directly related with the function of proliferation.
Zhang
Jing
J
Center of Reproduction, School of Medicine, Nanchang University, Nanchang 330006, China.
Zheng
Yue-Hui
YH
Zheng
Li-Ping
LP
D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D010053:Ovary / Q000201:enzymology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019098:Telomerase / Q000187:drug effects / Q000502:physiology*; D014700:Verapamil / Q000494:pharmacology
D006063:Chorionic Gonadotropin; D005640:Follicle Stimulating Hormone; D014700:Verapamil; D019098:Telomerase