title: Growth of iron-oxidizing thiobacilli in the presence of chalcopyrite and galena.
abstract: Iron-oxidizing thiobacilli were adapted to grow on a chalcopyrite and a galena ore concentrate. When grown on the chalcopyrite concentrate, the bacteria exhibited a doubling time of 38.4 +/- 2.9 h, with a final cellular protein concentration of 185 mug/ml and solubilization of 10.3 g of copper per liter. When grown on the galena ore concentrate, the generation time was 39.6 +/- 2.7 h, with a final cellular protein concentration of 120 mug/ml. Galena was converted to lead salts soluble in 1 M ammonium acetate to a concentration of 20.2 g of lead per liter. X-ray diffraction and refractive-image analysis indicated that the smaller-sized particles were favored in this process. Galena was converted to anglesite, and soluble copper was liberated from chalcopyrite with the concurrent formation of jarosite.
authors.affiliation: Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Québec, Canada, G1K 7P4.
authors.forename: J G
authors.initials: JG
authors.lastname: Kingma
authors.forename: M
authors.initials: M
authors.lastname: Silver