Hyperproduction of IFN-gamma by CpG oligodeoxynucleotide-induced exacerbation of atopic dermatitis-like skin lesion in some NC/Nga mice.
Under conventional conditions, NC/Nga mice spontaneously develop an atopic dermatitis (AD)-like skin lesion accompanied by immunoglobulin E (IgE) hyperproduction and the expression of T helper 2 (Th2) cytokines. CpG DNA activates a strong interferon-gamma (IFN-gamma)-dominated T helper 1 (Th1) response, while inhibiting Th2-dependent allergies. In this study, we examined whether CpG oligodeoxynucleotide (ODN) could prevent the development of the skin lesions in NC/Nga mice. Sixteen of 26 NC/Nga mice did not exhibit dermatitis after CpG ODN was administered intraperitoneally every 2 wk for a total of five times. CpG ODN administration induced IFN-gamma production, which inhibited the production of Th2 cytokines (interleukin (IL)-4, IL-5, and IL-13) in both spleen and lymph node cells and culminated in a decrease in the serum IgE level. These data suggest that the CpG ODN has a therapeutic effect against AD; however, some mice (10 of 26) treated with CpG ODN exhibited an exacerbation of dermatitis accompanied by the hyperproduction of IFN-gamma, although Th2 cytokines were suppressed. These results suggest that the suppression of Th2 cytokines may not completely prevent dermatitis and that IFN-gamma may play a role in developing dermatitis in some NC/Nga mice.
Takakura
Momoko
M
Department of Environmental Immuno-Dermatology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
Takeshita
Fumihiko
F
Aihara
Michiko
M
Xin
Ke-Qin
KQ
Ichino
Motohide
M
Okuda
Kenji
K
Ikezawa
Zenro
Z
D000818:Animals; D016207:Cytokines / Q000276:immunology; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D015226:Dinucleoside Phosphates; D004195:Disease Models, Animal; D007073:Immunoglobulin E / Q000097:blood*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D012871:Skin Diseases / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000276:immunology
D016207:Cytokines; D015226:Dinucleoside Phosphates; D007073:Immunoglobulin E; D007371:Interferon-gamma; D009838:Oligodeoxyribonucleotides; C015772:CpG dinucleotide