Production of auxin and related compounds by the plant parasitic nematodes Heterodera schachtii and Meloidogyne incognita.
Mass spectrometric analysis revealed the presence of auxin, mainly in conjugated form, in secretions of Heterodera schachtii and Meloidogyne incognita, with or without treatment with DMT or resorcinol. M. incognita showed the highest production rates, though treatment of M. incognita with resorcinol had a negative effect on auxin production. Analysis of auxin precursor molecules in lysates of H. schachtii, M. incognita and Caenorhabditis elegans suggested that auxin is most probably a degradation product of tryptophan and that auxin may be synthesized via several intermediates, including indole-3-acetamide which is an intermediate of a pathway so far only characterized in bacteria. Furthermore, high levels of anthranilate, a degradation product of tryptophan in animals, but possibly also a precursor for auxin were detected.
De Meutter
J
J
Vakgroep Moleculaire Genetica, Departement Plantensysteembiologie, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, Belgium.
Tytgat
T
T
Prinsen
E
E
Gheysen
G
G
Van Onckelen
H
H
Gheysen
G
G
D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism; D018556:Crops, Agricultural / Q000469:parasitology*; D006790:Host-Parasite Interactions; D007210:Indoleacetic Acids / Q000032:analysis* / Q000378:metabolism; D013058:Mass Spectrometry; D010937:Plant Growth Regulators / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D014431:Tylenchoidea / Q000378:metabolism*
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators