title: Preparation and Purification of Xylitol-5-Phosphate from a Cell Extract of Lactobacillus casei Cl-16.
abstract: A simple procedure which yields pure xylitol-5-phosphate is described. A cell extract of Lactobacillus casei Cl-16 from a 6-liter culture was used to synthesize up to 70 mg of xylitol-5-phosphate overnight from xylitol and phosphoenolpyruvate via a xylitol phosphoenolpyruvate:phosphotransferase system with a 53% yield. Centrifugation, filtration, precipitation as a barium salt, and ion-exchange batch chromatography permitted recovery of nearly 90% of the phosphorylated product synthesized. Thin-layer chromatography and enzymatic analysis indicated a purity level of more than 99%. The method was used to synthesize [U-C]xylitol-5-phosphate, and it is suitable for the synthesis of many other nonmetabolizable sugar phosphates.
authors.affiliation: Groupe de Recherche en Ecologie Buccale, Ecole de Médecine Dentaire, Université Laval, Ste.-Foy, Quebec G1K 7P4, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Trahan
authors.forename: S
authors.initials: S
authors.lastname: Néron
authors.forename: M
authors.initials: M
authors.lastname: Bareil