title: Effect of Temperature and Prey Availability on Growth of Paramoeba invadens in Monoxenic Culture.
abstract: Paramoeba invadens Jones 1985 is a pathogenic marine amoeba responsible for mass mortalities of sea urchins (Strongylocentrotus droebachiensis) of Nova Scotia between 1980 and 1983. A direct relationship between temperature and sea urchin paramoebiasis has been shown in previous laboratory and field studies. This study examined the effect of prey availability and temperature on the growth of P. invadens in monoxenic culture (with the marine bacterium Pseudomonas nautica). At 15 degrees C, the specific growth rate of P. invadens increased with bacterial prey concentration and was highest at 10 bacterial cells ml. Growth rate of P. invadens was maximal at 15 to 20 degrees C (which corresponds to annual sea temperature maxima in the natural environment) and the minimum generation time was 19.41 h at 20 degrees C. At 10 and 12 degrees C, generation times were 91.18 and 73.39 h, respectively; at 2 and 5 degrees C, there was no growth. P. invadens did not survive in monoxenic culture at 27 degrees C. Growth rates of P. invadens in vitro were positively correlated with time to morbidity of infected S. droebachiensis.
authors.affiliation: Biology Department, Dalhousie University, Halifax, Nova Scotia B3M 4J1, Canada.
authors.forename: J F
authors.initials: JF
authors.lastname: Jellett
authors.forename: R E
authors.initials: RE
authors.lastname: Scheibling