RNA GG x UU motif binds K+ but not Mg2+.
A hairpin model of the group I intron P5b loop was synthesized with [8-13C-7-15N]-guanosine in the GG.UU metal binding site, [7-15N]-guanosine at a nonbinding site, and [3-15N]-uridine. 15N NMR showed specific binding for Co(NH3)63+ and K+, but not for Zn2+, Cd2+, or Mg2+.
Fan
Yupeng
Y
Department of Chemistry and Chemical Biology, 610 Taylor Road, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA.
Gaffney
Barbara L
BL
Jones
Roger A
RA
D000818:Animals; D020029:Base Pairing; D001665:Binding Sites; D002412:Cations; D006147:Guanine / Q000737:chemistry / Q000378:metabolism*; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D011188:Potassium / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013768:Tetrahymena / Q000235:genetics; D014498:Uracil / Q000737:chemistry / Q000378:metabolism*
D002412:Cations; D009587:Nitrogen Isotopes; D014498:Uracil; D006147:Guanine; D012313:RNA; D008274:Magnesium; D011188:Potassium