[Application of Helena SAS-3 full automatic hemoglobin agarose electrophoresis].
OBJECTIVE
To assess the value of Halena SAS-3 full automatic hemoglobin agarose electrophoresis in the diagnosis of thalassemia.


METHODS
Hemoglobin A and A2 was detected by agarose electrophoresis in 200 blood samples collected from 170 healthy subjects, 2 patients with beta-thalassemia, 12 with acute lymphoblastic leukemia, 12 acute myelocytic leukemia and with 4 non-Hodgkin lymphoma.


RESULTS
The Mean+/-SD of hemoglobin A in the healthy subjects and patients with beta-thalassemia, acute lymphoblastic leukemia, acute myelocytic leukemia and non-Hodgkin lymphoma was 97.55+/-0.51, 97.01+/-0.329, 97.42+/-0.57, 97.44+/-0.55, and that of A2 was 2.44+/-0.51, 2.99+/-0.32, 2.84+/-0.28, 2.55+/-0.55, respectively. The values of electrophoresed hemoglobin F of the 2 patients with beta-thalassemia were higher than those of the normal adults. The within-run coefficient of variation (CV) and between-run CV was 0.17 and 7.76 for hemoglobin A, and 0.16 and 7.14 for hemoglobin A2, respectively.


CONCLUSIONS
Halena SAS-3 full automatic agarose electrophoresis system possesses strong resolving power in detecting trace abnormal hemoglobin and accurately identifies unusual evaluation of hemoglobin A2 and F to facilitate the diagnosis of thalassemia.
Mo
Jian-kun
JK
Clinical Laboratory, Second People's Hospital of Guangdong Province, Guangzhou 510310, China. mojiankun@21cn.com
Zhang
Zhi
Z
Li
Yong-xin
YX
Lei
Da
D
Ma
Jie
J
D002675:Child, Preschool; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005260:Female; D005319:Fetal Hemoglobin / Q000032:analysis; D006441:Hemoglobin A / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008297:Male; D017086:beta-Thalassemia / Q000097:blood*
D006454:Hemoglobins; D006441:Hemoglobin A; D005319:Fetal Hemoglobin