[Changes in composition of acid soluble proteins and DNA in chromatin of rat liver and brain bound and not bound to nuclear envelope as a function of age and under the influence of antioxidant ionol].
In two-day rat pups, the histone H1 content in the brain chromatin was higher than in the liver chromatin, as compared to histone of the nucleosome core. The H1 content in the brain chromatin decreased with the age, while in the liver chromatin it increased. At the same time, in the adult brain chromatin bound to the nuclear envelope, a high level of H1 characteristic of chromatin of the newborn rats was preserved, while in a similar chromatin of the adult liver, the H1 content increased, but still remained less than in the chromatin not bound to the nuclear envelope. In both organs, the composition and quantitation of H1 subfractions were different in chromatins bound and not bound to the nuclear envelope. The chromatin from the liver and brain bound to the nuclear envelope differed also in the composition and quantitation of minor acid soluble proteins. In the presence of the antioxidant ionol, the 5-methylcytosine content in DNA of chromatin of the rat liver bound to the nuclear envelope increased while in the chromatin not bound to the nuclear envelope, it remained unchanged. Thus the chromatins bound and not bound to the nuclear envelope differ in the composition and mount of acid soluble proteins, including histone H1, the contents of these proteins in bound and not bound chromatin are different and change with the age in different ways. The antioxidant ionol affects differently the methylation of bound and not bound chromatin.
Prusov
A N
AN
Romanenko
E B
EB
Vaniushin
B F
BF
D000375:Aging / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000008:administration & dosage*; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D001923:Brain Chemistry / Q000187:drug effects; D002084:Butylated Hydroxytoluene / Q000008:administration & dosage*; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D019175:DNA Methylation / Q000187:drug effects; D005260:Female; D006657:Histones / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D009685:Nuclear Envelope / Q000648:ultrastructure; D051381:Rats
D000975:Antioxidants; D002843:Chromatin; D006657:Histones; D002084:Butylated Hydroxytoluene; D004247:DNA