title: Enhanced transformation and chemosensitivity of NIH3T3 cells transduced with hepatoma up-regulated protein.
abstract: Hepatoma up-regulated protein (HURP) is a recently identified novel cell-cycle-regulated gene. The HURP gene is overexpressed in human hepatocellular carcinoma and transitional cell carcinoma. The cellular function of HURP is not fully understood. In this study, the NIH3T3 cells transduced with the exogenous HURP gene manifested the general characteristics of tumor cells, which had higher growth rate in low-serum media and advanced ability of colony formation on agarose-based plates. Transduced HURP was capable of specifically enhancing the chemosensitivity of deoxycytosine analogs, such as gemcitabine, ARA-C, and 5-AZA-CdR, but neither had an effect on the response of DNA intercalating agents, such as cisplatin, carboplatin, and doxorubicin, nor on the response of microtubule stabilizers, such as paclitaxel, docetaxel, and vinblastine. These results indicate that the HURP gene might be a potential oncogenic gene and capable of enhancing the chemosensitivity of deoxycytosine analogs in NIH3T3 cells.
authors.affiliation: Department of Medical Research, Chi-Mei Medical Center, Chung Hwa College of Medical Technology, Tainan, Taiwan.
authors.forename: Yu-Chun
authors.initials: YC
authors.lastname: Wang
authors.forename: Ying-Huei
authors.initials: YH
authors.lastname: Lee
authors.forename: Guan-Cheng
authors.initials: GC
authors.lastname: Huang
authors.forename: Yu-hsuan
authors.initials: YH
authors.lastname: Lin
authors.forename: Mei Huei
authors.initials: MH
authors.lastname: Fan-Chiang
authors.forename: Allen W
authors.initials: AW
authors.lastname: Chiu
authors.forename: Yu-Lun
authors.initials: YL
authors.lastname: Huang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007928:Lethal Dose 50; D051379:Mice; D041681:NIH 3T3 Cells; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D000970:Antineoplastic Agents; C465041:DLGAP5 protein, human; D009363:Neoplasm Proteins; D011994:Recombinant Proteins