title: No association between transmembrane protein-tyrosine phosphatase receptor type C (CD45) exon A point mutation (77C>G) and idiopathic dilated cardiomyopathy.
abstract: To investigate whether a 77C>G polymorphism in exon A of the CD45 gene causing a variant CD45RA expression pattern is associated with the development of idiopathic dilated cardiomyopathy (DCM), we studied a total of 414 individuals (104 patients and 310 controls). CD45RA expression pattern on lymphocytes was examined by flow cytometric analysis and subsequently the CD45 77C>G polymorphism was genotyped by polymerase chain reaction-allele specific restriction enzyme analysis (PCR-ASRA). We found 5 patients and 8 control individuals displaying the variant CD45RA expression pattern. All identified individuals carried the heterozygous CD45 77C>G polymorphism. The frequency of the 77G allele in the patient group was 2.4%, which was not significantly different from 1.3% found in the control group (p=0.327). In conclusion, the data of this preliminary study could not reveal any association between the CD45 77C>G polymorphism and susceptibility to idiopathic DCM in a German population.
authors.affiliation: Institute for Transfusion Medicine, University Hospital of Jena, Jena, Germany. Hansjoerg.Thude@med.uni-jena.de
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Thude
authors.forename: Konstanze
authors.initials: K
authors.lastname: Gerlach
authors.forename: Barbara
authors.initials: B
authors.lastname: Richartz
authors.forename: Andreas
authors.initials: A
authors.lastname: Krack
authors.forename: Bettina
authors.initials: B
authors.lastname: Brenke
authors.forename: Klaus
authors.initials: K
authors.lastname: Pethig
authors.forename: Hans-Reiner
authors.initials: HR
authors.lastname: Figulla
authors.forename: Dagmar
authors.initials: D
authors.lastname: Barz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002311:Cardiomyopathy, Dilated / Q000235:genetics* / Q000276:immunology; D005091:Exons; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D017493:Leukocyte Common Antigens / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D017354:Point Mutation
chemical_list: D017493:Leukocyte Common Antigens