Nitrogen Fixation in Continuous Culture with NH(4)Cl-Containing Media.
N balance and N dilution were determined from growth of Azospirillum brasilense Sp7 and two unidentified gram-negative nitrogen-fixing microorganisms in continuous culture supplied with NH(4)Cl. At the 1.1 and 2.2 mM NH(4)Cl steady states (N-to-C ratios of 1:68 and 1:34, respectively), the organisms grew with NH(4)Cl and N(2) as N sources simultaneously under carbon limitation. No ammonium could be detected in the supernatant of these cultures.
Fritzsche
C
C
Institute of Biophysics, University of Hannover, Herrenhaeuser Strasse 2, 3000 Hannover 21, Federal Republic of Germany.
Niemann
E G
EG