title: Synthesis, processing, and composition of the virion-associated HTLV-1 reverse transcriptase.
abstract: It is not known whether the low infectivity and low virion-associated polymerase activity of human T-cell lymphotropic virus type-1 (HTLV-1) are due to the quantity or quality of the reverse transcriptase (RT), because the protein has not yet been fully characterized. We have developed anti-RT antibodies and constructed HTLV-1 expression plasmids that express truncated or hemagglutinin-tagged Pol polyproteins to examine the maturation and composition of HTLV-1 RT. We detected virion-associated proteins corresponding to RT-integrase (IN) (pr98) and RT (p62) as well as smaller proteins containing the polymerase (p49) or RNase H domains. We have identified the amino acid sequences in the Pol polyprotein that are cleaved by HTLV-1 protease to yield RT and IN. We have also identified the cleavage sites within RT that give rise to the p49 polymerase fragment. Immunoprecipitation of an epitope-tagged p62 subunit coprecipitated p49, indicating that the HTLV-1 RT complex can exist as a p62/p49 heterodimer analogous to the RT of HIV-1 (p66/p51).
authors.affiliation: HIV Drug Resistance Program, NCI-Frederick, Maryland 21702, USA.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Mitchell
authors.forename: József
authors.initials: J
authors.lastname: Tözsér
authors.forename: Gerald
authors.initials: G
authors.lastname: Princler
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Lloyd
authors.forename: Ashleigh
authors.initials: A
authors.lastname: Auth
authors.forename: David
authors.initials: D
authors.lastname: Derse
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D019281:Dimerization; D015368:Human T-lymphotropic virus 1 / Q000201:enzymology*; D006801:Humans; D019426:Integrases / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D014771:Virion / Q000201:enzymology*
chemical_list: D019426:Integrases; D012194:RNA-Directed DNA Polymerase