title: Structural analysis of kappa-carrageenan [corrected] sulfated oligosaccharides by positive mode nano-ESI-FTICR-MS and MS/MS by SORI-CID.
abstract: Structural analysis of sulfated oligosaccharides from kappa-carrageenan of up to ten residues (MW >2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.
authors.affiliation: Department of Chemistry, Ateneo de Manila University, Quezon City, Philippines.
authors.forename: Jennifer T
authors.initials: JT
authors.lastname: Aguilan
authors.forename: Fabian M
authors.initials: FM
authors.lastname: Dayrit
authors.forename: Jinhua
authors.initials: J
authors.lastname: Zhang
authors.forename: Milady R
authors.initials: MR
authors.lastname: Niñonuevo
authors.forename: Carlito B
authors.initials: CB
authors.lastname: Lebrilla
mesh_terms: D002240:Carbohydrate Sequence; D002351:Carrageenan / Q000737:chemistry*; D005583:Fourier Analysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization; D013431:Sulfates / Q000737:chemistry
chemical_list: D009844:Oligosaccharides; D013431:Sulfates; D002351:Carrageenan