Preparation of poly(ethylene glycol)-introduced cationized gelatin as a non-viral gene carrier.
The objective of this study was to prepare cationized gelatins grafted with poly(ethylene glycol) (PEG) (PEG-cationized gelatin) and evaluate the in vivo efficiency as a non-viral gene carrier. Cationized gelatin was prepared by chemical introduction of ethylenediamine to the carboxyl groups of gelatin. PEG with one terminal of active ester group was coupled to the amino groups of cationized gelatin to prepare PEG-cationized gelatins. Electrophoretic experiments revealed that the PEG-cationized gelatin with low PEGylation degrees was complexed with a plasmid DNA of luciferase, in remarked contrast to that with high PEGylation degrees. When the plasmid DNA complexed with the cationized gelatin or PEG-cationized gelatin was mixed with deoxyribonuclease I (DNase I) in solution to evaluate the resistance to enzymatic degradation, stronger protection effect of the PEG-cationized gelatin was observed than that of the cationized gelatin. The complex of plasmid DNA and PEG-cationized gelatin had an apparent molecular size of about 300 nm and almost zero surface charge. These findings indicate that the PEG-cationized gelatin-plasmid DNA complex has a nano-order structure where the plasmid DNA is covered with PEG molecules. When the PEG-cationized gelatin-plasmid DNA complex was intramuscularly injected, the level of gene expression was significantly increased compared with the injection of plasmid DNA solution. It is concluded that the PEG-cationized gelatin was a promising non-viral gene carrier to enhance gene expression in vivo.
Kushibiki
Toshihiro
T
Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Tabata
Yasuhiko
Y
D000818:Animals; D002412:Cations; D004247:DNA / Q000737:chemistry; D003850:Deoxyribonuclease I / Q000737:chemistry; D016503:Drug Delivery Systems; D004586:Electrophoresis; D005780:Gelatin / Q000737:chemistry*; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D005822:Genetic Vectors; D020100:Hydrogels / Q000737:chemistry; D008027:Light; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D010957:Plasmids / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine; D012542:Scattering, Radiation; D013997:Time Factors; D014162:Transfection
D002412:Cations; D020100:Hydrogels; D011107:Polylysine; D011092:Polyethylene Glycols; D005780:Gelatin; D004247:DNA; D008156:Luciferases; D003850:Deoxyribonuclease I