Faster axial band dispersion in a monolithic silica column than in a particle-packed column.
The contribution of molecular diffusion to peak broadening was studied in a reversed-phase HPLC system, consisting of a monolithic silica C18 column and methanol-water mobile phase. Study on the band broadening effect of holding a solute in a column or elution at very low linear velocity of mobile phase allowed facile determination of the contribution of the molecular diffusion term. Less obstruction against molecular diffusion, or the faster axial band dispersion in a monolithic silica column than in a particle-packed column, was found both in mobile phase and in stationary phase.
Kobayashi
Hiroshi
H
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Tokuda
Daisuke
D
Ichimaru
Jun
J
Ikegami
Tohru
T
Miyabe
Kanji
K
Tanaka
Nobuo
N
D001555:Benzene Derivatives / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D004058:Diffusion; D009930:Organic Chemicals / Q000302:isolation & purification; D012822:Silicon Dioxide; D014498:Uracil / Q000302:isolation & purification
D001555:Benzene Derivatives; D009930:Organic Chemicals; C024268:n-propylbenzene; D014498:Uracil; D012822:Silicon Dioxide; C053762:n-hexylbenzene