Fractality of dendritic arborization of spinal cord neurons.
Skeletonized images of Golgi impregnated neurons from the human, monkey, cat and rat dorsal horns were subjected to fractal analysis. These neurons have sparse branching of dendrite arbors. It is noticed that, in certain neuronal samples, some authors report that scaling range of experimentally declared fractals is extremely limited and spanned approximately between 0.5 and 2.0 decades. In order to retain our hypothesis that neurons with dendrites of uncomplicated shapes can be considered fractal over three decades of scale, we conducted four procedures: (i) we used the box-counting method, (ii) we scaled the box sizes as a power of 2, (iii) we chose the coefficient of correlation, measuring the "goodness of fit" of experimental data points to regression straight line, to be equal to or larger than 0.995, and (iv) we pointed out that all the neurons analyzed have a single fractal dimension measuring a global fractality showing no linear regions. As a control, we used some cerebellar Purkinje cells whose dendrite trees show much more complex structure and profuseness of branching. Since, generally, the neuronal structure is among the most complex of all cellular morphologies, we believe that supporting this hypothesis we advance the neuroscience and fractal theory.
Milosević
Nebojsa T
NT
Department of Biophysics, Faculty of Medicine, University of Belgrade, Visegradska 26/2, 11000 Belgrade, Serbia and Montenegro. mtn@sbb.co.yu
Ristanović
Dusan
D
D000818:Animals; D002415:Cats; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure; D000882:Haplorhini; D006801:Humans; D009474:Neurons / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D012044:Regression Analysis; D016622:Silver Staining / Q000379:methods; D013116:Spinal Cord / Q000166:cytology*