Scratching behavior in mice induced by the proteinase-activated receptor-2 agonist, SLIGRL-NH2.
We examined whether the proteinase-activated receptor-2 (PAR2) agonist, H-Ser-Leu-Ile-Gly-Arg-Leu-NH2 (SLIGRL-NH2), could induce scratching behavior in mice. Intradermal injections of SLIGRL-NH2 (10-50 microg) evoked dose dependent scratching. This behavior peaked near 5 min and returned to preinjection levels within 30 min. Pretreatment of animals with a histamine H1 receptor antagonist, pyrilamine, blocked histamine induced scratching, but it had little effect on SLIGRL scratching. Our study suggests that PAR2 mediates histamine independent itch.
Shimada
Steven G
SG
Department of Anesthesiology, Yale University School of Medicine, P.O. Box 208051, 333 Cedar Street, New Haven, CT 06520-8051, USA. steven.shimada@yale.edu
Shimada
Kelly A
KA
Collins
J G
JG
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009842:Oligopeptides / Q000494:pharmacology*; D011537:Pruritus / Q000139:chemically induced* / Q000503:physiopathology; D011738:Pyrilamine / Q000494:pharmacology; D044464:Receptor, PAR-2 / Q000819:agonists*
D006634:Histamine H1 Antagonists; D009842:Oligopeptides; D044464:Receptor, PAR-2; C112356:seryl-leucyl-isoleucyl-glycyl--arginyl-leucinamide; D011738:Pyrilamine