Chemiluminescent acridinium-9-carboxamide boronic acid probes: application to a homogeneous glycated hemoglobin assay.
Chemiluminescent acridinium-9-carboxamide probes containing 1, 3, 9, and 27 phenylboronic acids were prepared and their chemiluminescent properties evaluated. The relative chemiluminescent signal from the probes varied from 4 to 0.83 x 10(19)counts/mol across the series, while the apparent affinity of the probes for the diabetes marker glycated hemoglobin increased from 211 to 0.43 microM. The dose-dependent modulation of the chemiluminescent intensity of the probes upon binding was used to demonstrate a homogeneous assay for glycated hemoglobin.
Adamczyk
Maciej
M
Abbott Laboratories, Diagnostics Division, 100 Abbott Park Road, AP20, Abbott Park, IL 60064-6016, USA. maciej.adamcyzk@abbott.com
Chen
Yon-Yih
YY
Johnson
Donald D
DD
Mattingly
Phillip G
PG
Moore
Jeffrey A
JA
Pan
You
Y
Reddy
Rajarathnam E
RE
D000166:Acridines / Q000032:analysis* / Q000737:chemistry*; D001897:Boronic Acids / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D006442:Glycated Hemoglobin / Q000032:analysis*; D008163:Luminescent Measurements / Q000379:methods*; D015394:Molecular Structure
D000166:Acridines; D001897:Boronic Acids; D006442:Glycated Hemoglobin; C402305:acridinium-9-carboxamide