Could the homologous sequence of anti-inflammatory pentapeptide (MLIF) produced by Entamoeba histolytica in the N protein of rabies virus affect the inflammatory process?
Amebiasis and rabies are public health problems, and they have in common a poor inflammatory effect in the target organs that they affect. In the GenBank, it was found that the anti-inflammatory peptide monocyte locomotion inhibitory factor (MLIF) produced by Entamoeba histolytica homologates 80%, with a fragment of the N protein of the rabies virus. We speculated if the N protein could contribute to the scant inflammatory reaction produced by rabies virus in central nervous system. The N protein was obtained and studied in vitro and in vivo. The N protein, as MLIF, inhibited the respiratory burst in human mononuclear phagocytes (43%, p<0.05), but in contrast to MLIF, it increased chemotaxis and it did not significantly inhibit delayed hypersensitivity skin reaction to 1-chloro-2-4-dinitrobenzene in guinea pigs. Therefore, the full peptide sequence has to be present or it has to be cleaved-free from the large recombinant N protein molecule (55 kDa) to become active.
Morales
M E
ME
Unidad de Investigación Médica en Inmunología, Unidad Médica de Alta Especialidad, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Mexico City, DF, Mexico. estmormag@hotmail.com
Rico
G
G
Gómez
J L
JL
Alonso
R
R
Cortés
R
R
Silva
R
R
Giménez
J A
JA
Kretschmer
R
R
Aguilar-Setién
A
A
D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D002630:Chemotactic Factors; D004748:Entamoeba histolytica / Q000187:drug effects / Q000378:metabolism*; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D007249:Inflammation / Q000378:metabolism*; D009036:Moths / Q000166:cytology; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism*; D016897:Respiratory Burst
D002630:Chemotactic Factors; D019590:Nucleocapsid Proteins; D009842:Oligopeptides; D015800:Protozoan Proteins; C430647:monocyte locomotion inhibitory factor; C099601:nucleocapsid protein, Rabies virus