title: Cloning of Protocatechuate 3,4-Dioxygenase Genes from Bradyrhizobium japonicum USDA110.
abstract: A heterologous gene probe encoding the alpha and beta subunits of the Pseudomonas cepacia protocatechuate 3,4-dioxygenase (PCD) was used to detect its homolog in the genome of Bradyrhizobium japonicum USDA110. Three cosmid clones carrying a 2.2-kb BamHI insert showed high levels of PCD activity. SacI digestion of one of the genomic clones, pBjG17, produced a 2.5-kb insert DNA that complemented a PCD mutant of P. cepacia.
authors.affiliation: Department of Biological Sciences, Michigan Technological University, Houghton, Michigan 49931, and Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Hyattsville, Maryland 20782.
authors.forename: G K
authors.initials: GK
authors.lastname: Podila
authors.forename: S
authors.initials: S
authors.lastname: Kotagiri
authors.forename: S
authors.initials: S
authors.lastname: Shantharam