Construction and characterization of a series of vectors for Schizosaccharomyces pombe.
A set of vectors was created to allow cloning and expression studies in Schizosaccharomyces pombe. These vectors had a uniform backbone with an efficient Sz. pombe ARS, ARS3002, but different selectable markers--his3+, leu1+, ade6+ and ura4+. The vectors functioned efficiently as autonomously replicating plasmids that could also be converted into integrating vectors. The ura4+-containing vector was used to construct a Sz. pombe genomic library.
Adams
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Unit on Chromatin and Transcription, NICHD/NIH, Building 18T, Room 106, 18 Library Drive, Bethesda, MD 20892, USA.
Haldar
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Kamakaka
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D015723:Gene Library; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000138:chemical synthesis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*
D005819:Genetic Markers