Continuous Cultivation for Apparent Optimization of Defined Media for Cellulomonas sp. and Bacillus cereus.
Steady-state continuous culture was used to optimize lean chemically defined media for a Cellulomonas sp. and Bacillus cereus strain T. Both organisms were extremely sensitive to variations in trace-metal concentrations. However, medium optimization by this technique proved rapid, and multifactor screening was easily conducted by using a minimum of instrumentation. The optimized media supported critical dilution rates of 0.571 and 0.467 h for Cellulomonas and Bacillus, respectively. These values approximated maximum growth rate values observed in batch culture.
Summers
R J
RJ
Department of Microbiology, Louisiana State University, Baton Rouge, Louisiana 70803.
Boudreaux
D P
DP
Srinivasan
V R
VR