title: Toxicity of 1,1,1-Trichloroethane and Trichloroethene on a Mixed Culture of Methane-Oxidizing Bacteria.
abstract: The influence of trichloroethene (TCE; 0 to 65 mg/liter) and 1,1,1-trichloroethane (1,1,1-TCA; 0 to 103 mg/liter) on methane consumption of a mixed culture of methane-oxidizing bacteria was studied in laboratory batch experiments. Increasing concentrations of TCE or 1,1,1-TCA resulted in decreasing methane consumption. Methane consumption was totally inhibited at a concentration of 13 mg of TCE per liter, while methane consumption was still observed at the upper studied concentration of 103 mg of 1,1,1-TCA per liter. The inhibition of methane consumption by TCE depended on the initial concentration of methane. A model accounting for competitive inhibition between methane and TCE or 1,1,1-TCA was used to simulate methane consumption at various concentrations of TCE or 1,1,1-TCA. The simulations indicated that competitive inhibition may be the mechanism causing the inhibitory effect of TCE on methane consumption, while this does not seem to be the case for 1,1,1-TCA.
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, DK-2800 Lyngby, Denmark.
authors.forename: Kim
authors.initials: K
authors.lastname: Broholm
authors.forename: Bjørn K
authors.initials: BK
authors.lastname: Jensen
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Christensen
authors.forename: Lajla
authors.initials: L
authors.lastname: Olsen