title: Interleukin-2 and interleukin-6 gene promoter polymorphisms, and early failure of dental implants.
abstract: Single nucleotide polymorphisms in the promoter region of the human interleukin (IL)-2 (T-330G) and IL-6 (G-174C) genes have modified the transcriptional activity of these cytokines and are associated with several diseases. The aim of this study was to investigate the possible relationship between these single nucleotide polymorphisms and early implant failure. A sample of 74 nonsmokers was divided into 2 groups: test group comprising 34 patients (mean age 49.3 years) with >or=1 implants that failed and control group consisting of 40 patients (mean age 43.8 years) with >or=1 healthy implants. Genomic deoxyribonucleic acid from oral mucosa was amplified by polymerase chain reaction and analyzed by restriction fragment length polymorphism. Monte Carlo simulations (P < 0.05) were used to assess differences in allele and genotypes frequencies of the single nucleotide polymorphisms between the 2 groups. No significant differences were observed in the allele and genotypes distribution of both polymorphisms when the 2 groups were compared. The results indicate that polymorphisms in the IL-2 (T-330G) and IL-6 (G-174C) genes are not associated with early implant failure, suggesting that the presence of those single nucleotide polymorphisms does not constitute a genetic risk factor for implant loss in the studied population.
authors.affiliation: Department of Morphology, Dental School of Piracicaba, State University of Campinas, Piracicaba, Brazil.
authors.forename: Maria Isabela Guimarães
authors.initials: MI
authors.lastname: Campos
authors.forename: Maria Cristina Leme
authors.initials: MC
authors.lastname: Godoy dos Santos
authors.forename: Paula Cristina
authors.initials: PC
authors.lastname: Trevilatto
authors.forename: Raquel Mantuaneli
authors.initials: RM
authors.lastname: Scarel-Caminaga
authors.forename: Fabio Jose
authors.initials: FJ
authors.lastname: Bezerra
authors.forename: Sergio Roberto Peres
authors.initials: SR
authors.lastname: Line
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D015921:Dental Implants; D019232:Dental Restoration Failure; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D013997:Time Factors
chemical_list: D015921:Dental Implants; D007376:Interleukin-2; D015850:Interleukin-6