The isolation and characterisation of primary human laryngeal epithelial cells.
We have shown that the larynx has a prominent immunological component that varies between individuals, and which is influenced by lifestyle factors implicated in the pathogenesis of the inflammatory and neoplastic diseases of the larynx. In order to explore the mechanisms of such links between laryngeal mucosal immunity and the development of lifestyle-related disease, reliable in vitro models are essential. In this study, we isolated and characterised primary laryngeal epithelial cells from normal individuals and show they can be cultured and manipulated to express MHC class II molecules in vitro.
Rees
Louisa E N
LE
Laryngeal Research Group, Division of Veterinary Pathology, Infection and Immunity, Clinical Veterinary Science, University of Bristol, Bristol, UK. l.e.rees@bristol.ac.uk
Gunasekaran
Sinnappa
S
Sipaul
Fabian
F
Birchall
Martin A
MA
Bailey
Michael
M
D018929:Cell Culture Techniques; D002469:Cell Separation; D048430:Cell Shape; D002478:Cells, Cultured; D006682:HLA-DP Antigens / Q000032:analysis; D006683:HLA-DQ Antigens / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D006801:Humans; D007109:Immunity; D007820:Laryngeal Mucosa / Q000166:cytology* / Q000276:immunology*
D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II