title: Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent.
abstract: Harmful algal blooms, caused by rapid growth and accumulation of certain microalgae in the ocean, pose considerable impacts on marine environments, aquatic industries and even public health. Here, we present the 7.2-megabase genome of the marine bacterium Hahella chejuensis including genes responsible for the biosynthesis of a pigment which has the lytic activity against a red-tide dinoflagellate. H.chejuensis is the first sequenced species in the Oceanospiralles clade, and sequence analysis revealed its distant relationship to the Pseudomonas group. The genome was well equipped with genes for basic metabolic capabilities and contained a large number of genes involved in regulation or transport as well as with characteristics as a marine heterotroph. Sequence analysis also revealed a multitude of genes of functional equivalence or of possible foreign origin. Functions encoded in the genomic islands include biosynthesis of exopolysacchrides, toxins, polyketides or non-ribosomal peptides, iron utilization, motility, type III protein secretion and pigmentation. Molecular structure of the algicidal pigment, which was determined through LC-ESI-MS/MS and NMR analyses, indicated that it is prodigiosin. In conclusion, our work provides new insights into mitigating algal blooms in addition to genetic make-up, physiology, biotic interactions and biological roles in the community of a marine bacterium.
authors.affiliation: Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 305-600, Republic of Korea.
authors.forename: Haeyoung
authors.initials: H
authors.lastname: Jeong
authors.forename: Joung Han
authors.initials: JH
authors.lastname: Yim
authors.forename: Choonghwan
authors.initials: C
authors.lastname: Lee
authors.forename: Sang-Haeng
authors.initials: SH
authors.lastname: Choi
authors.forename: Yon Kyoung
authors.initials: YK
authors.lastname: Park
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Yoon
authors.forename: Cheol-Goo
authors.initials: CG
authors.lastname: Hur
authors.forename: Ho-Young
authors.initials: HY
authors.lastname: Kang
authors.forename: Dockyu
authors.initials: D
authors.lastname: Kim
authors.forename: Hyun Hee
authors.initials: HH
authors.lastname: Lee
authors.forename: Kyun Hyang
authors.initials: KH
authors.lastname: Park
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Park
authors.forename: Hong-Seog
authors.initials: HS
authors.lastname: Park
authors.forename: Hong Kum
authors.initials: HK
authors.lastname: Lee
authors.forename: Tae Kwang
authors.initials: TK
authors.lastname: Oh
authors.forename: Jihyun F
authors.initials: JF
authors.lastname: Kim
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001483:Base Sequence; D004141:Dinoflagellida / Q000187:drug effects*; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D016680:Genome, Bacterial; D023281:Genomics; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010802:Phylogeny; D010839:Phytoplankton / Q000187:drug effects*; D011353:Prodigiosin / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D014774:Virulence / Q000235:genetics
chemical_list: D000981:Antiprotozoal Agents; D011353:Prodigiosin