Cloning and characterization of the Cu/Zn superoxide dismutase of Trichinella pseudospiralis.
Copper/zinc (Cu/Zn) superoxide dismutase (SOD) activity was identified for the first time in both crude somatic extracts (CE) and excretory/secretory (E/S) products of Trichinella pseudospiralis. It was the dominant SOD in infective-stage larvae. Native polyacrylamide gel electrophoresis of CE and E/S products yielded a prominent band, which was cyanide-sensitive and was partly inhibited by hydrogen peroxide in SOD assay. Cytosolic Cu/Zn SOD was cloned. The 471-bp full-length cDNA sequence contained an open reading frame of 157 amino acids. The gene contained three introns. Quantitative reverse transcription-polymerase chain reaction indicated that the expression of cytosolic Cu/Zn SOD was substantially higher in infective-stage larvae than in adult worms. Cluster analysis showed that the sequence of the Cu/Zn SOD of T. pseudospiralis, an adenophorean nematode, is related to those of Brugia pahangi, Acanthocheilonema viteae, Onchocerca volvulus, and Haemonchus contortus (all belonging to the sercenentean group).
Wu
W K
WK
Department of Zoology, The University of Hong Kong, Pokfulam, Hong Kong, SAR, China.
Mak
C H
CH
Ko
R C
RC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D017238:Genes, Helminth; D007814:Larva / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000235:genetics*; D014234:Trichinella / Q000145:classification / Q000201:enzymology* / Q000235:genetics
D018076:DNA, Complementary; D018509:DNA, Helminth; D013482:Superoxide Dismutase