title: Simple, sensitive zymogram technique for detection of xylanase activity in polyacrylamide gels.
abstract: A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands corresponding to enzymatic activity against an opaque background.
authors.affiliation: Department of Environmental and Forest Biology, College of Environmental Science and Forestry, State University of New York, Syracuse, New York 13210.
authors.forename: J C
authors.initials: JC
authors.lastname: Royer
authors.forename: J P
authors.initials: JP
authors.lastname: Nakas