title: H(2)-CO(2) Recirculation and pH Control for Growth of Methanogens in Mass Culture.
abstract: We modified a fermentor (10-liter liquid volume) for the growth of anaerobic, H(2)-CO(2)-catabolizing bacteria. Gas in the fermentor (ca. 10% CO(2), 50% H(2), 40% CH(4)) was recirculated by a diaphragm pump. During growth, the gas composition was maintained by the addition of a mixture of 80% H(2) and 20% CO(2), and this addition was controlled by a pH auxostat. During gas addition, gas was discharged from the recirculating gas stream and was collected by the displacement of an acidified salt solution.
authors.affiliation: Division of Environmental and Occupational Health Sciences, School of Public Health, University of California, Los Angeles, California 90024.
authors.forename: D R
authors.initials: DR
authors.lastname: Boone
authors.forename: I M
authors.initials: IM
authors.lastname: Mathrani
authors.forename: R A
authors.initials: RA
authors.lastname: Mah