title: Catabolism of substituted benzoic acids by streptomyces species.
abstract: Four thermotolerant actinomycetes from soil, identified as Streptomyces albulus 321, Streptomyces sioyaensis P5, Streptomyces viridosporus T7A, and Streptomyces sp. V7, were grown at 45 degrees C in media containing either benzoic acid or hydroxyl- and methoxyl-substituted benzoic acids as the principal carbon sources. Benzoic acid was converted to catechol; p-hydroxybenzoic, vanillic, and veratric acids were converted to protocatechuic acid; and m-hydroxybenzoic acid was converted to gentisic acid. Catechol, protocatechuic acid, and gentisic acid were cleaved by catechol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase, and gentisate 1,2-dioxygenase, respectively. Dioxygenases appeared only in induced cultures. m-Hydroxybenzoic, m-anisic, and p-anisic acids were gratuitous inducers of dioxygenases in some strains. One strain converted vanillic acid to guaiacol.
authors.affiliation: Department of Bacteriology and Biochemistry, Idaho Agricultural Experiment Station, University of Idaho, Moscow, Idaho 83843.
authors.forename: J B
authors.initials: JB
authors.lastname: Sutherland
authors.forename: D L
authors.initials: DL
authors.lastname: Crawford
authors.forename: A L
authors.initials: AL
authors.lastname: Pometto