DNA-intercalation on pyrene modified surface coatings.
Utilising the strong affinity between nucleic acids and an intercalating pyrene derivate, a novel efficient method for unspecific immobilisation of double-stranded DNA on to solid support for applications in bioanalytic, biophysics and microbiology is presented.
Laib
Stephan
S
Institute for Physical Chemistry, University of Zurich, Winterthurerstr. 190, 8057 Zurich, Switzerland. slaib@physics.ucsd.edu
Krieg
Alexander
A
Häfliger
Pascal
P
Agorastos
Nikos
N
D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D038761:Photobleaching; D011721:Pyrenes / Q000737:chemistry*
D011721:Pyrenes; D004247:DNA; C030984:pyrene