title: Predicting 2,4-dichlorophenoxyacetic Acid ester transformation rates in periphyton-dominated ecosystems.
abstract: Using batch cultures, we determined transformation rate coefficients for microbial transformation of 2,4-dichlorophenoxyacetic acid butoxyethyl ester (2,4-DBE) in periphyton-dominated ecosystems. Rates of 2,4-DBE loss were measured over short periods of time (usually less than 10 h), and first-order transformation rate coefficients (k(1)) were determined under the specific conditions of low 2,4-DBE concentrations and no growth. Values for k(1) were divided by total plate counts and by biomass measured as ash-free dry weight to give second-order rate coefficients (k(b) and k(AFDW), respectively) for use in predictive models. Using periphyton attached to Teflon strips, we also determined second-order rate coefficients based on the ratio of colonized surface area to container volume (k(A)). Mean second-order rate coefficients were used to predict 2,4-DBE transformation rates in microcosms having diverse chemical and biological environments. The observed transformation rates among the microcosms were most accurately predicted by using k(A).
authors.affiliation: U.S. Environmental Protection Agency, Environmental Research Laboratory, Athens, Georgia 30613.
authors.forename: D L
authors.initials: DL
authors.lastname: Lewis
authors.forename: H P
authors.initials: HP
authors.lastname: Kollig
authors.forename: T L
authors.initials: TL
authors.lastname: Hall