Core-shell microspheres by dispersion polymerization as promising delivery systems for proteins.
Functional poly(methyl methacrylate) core-shell microspheres were prepared by dispersion polymerization. An appropriate selection of experimental parameters and in particular of the initiator and stabilizer amount and of the medium solvency power allowed a monodisperse sample as large as 600 nm to be prepared. To this purpose, low initiator concentration, high steric stabilizer amount and a low solvency power medium were employed. The microspheres present a core-shell structure in which the outer shell is constituted by the steric stabilizer which affords carboxylic groups able to interact with basic proteins, such as trypsin, whose adsorption is essentially driven by the carboxylic group density in the microsphere shell. Finally, fluorescent microspheres were prepared for biodistribution studies and shown to be readily taken up by the cells both in vitro and in vivo. These results suggest that these microspheres are promising delivery systems for the development of novel protein-based vaccines.
Sparnacci
Katia
K
Department of Environmental and Life Sciences INSTM, University of Piemonte Orientale, Spalto Marengo 33, 15100 Alessandria, Italy.
Laus
Michele
M
Tondelli
Luisa
L
Bernardi
Cinzia
C
Magnani
Laura
L
Corticelli
Franco
F
Marchisio
Marco
M
Ensoli
Barbara
B
Castaldello
Arianna
A
Caputo
Antonella
A
D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004337:Drug Carriers; D005240:Feasibility Studies; D005260:Female; D019793:Fluorescein; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007211:Indoles; D007273:Injections, Intramuscular; D007526:Isoelectric Point; D051379:Mice; D008815:Mice, Inbred Strains; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D015394:Molecular Structure; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D010316:Particle Size; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000493:pharmacokinetics*; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics; D013499:Surface Properties; D013997:Time Factors; D014357:Trypsin / Q000737:chemistry / Q000493:pharmacokinetics
D000180:Acrylic Resins; D001672:Biocompatible Materials; D004337:Drug Carriers; C446821:Eudragit L100-55; D005456:Fluorescent Dyes; D007211:Indoles; D011506:Proteins; D012710:Serum Albumin, Bovine; C007293:DAPI; D019904:Polymethyl Methacrylate; D014357:Trypsin; D019793:Fluorescein