Comparative proteomic analysis of B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis.
OBJECTIVE
To compare the protein difference between B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis.


METHODS
Protein samples were prepared by vorterx, ultrasonic treatment, and centrifugation. Protein concentrations were determined by Bradford method. Protein difference was compared by the first IEF and the second SDS-polyacrylamide gel electrophoresis.


RESULTS
Protein concentrations in samples of Bartonella henselae Houston and Bartonella henselae Marseille were 2.117 microg/microL and 2.200 microg/microL respectively. Sample protein of 40 microg for IPG strips loading was perfect. The results of 2-DE in pH 4 to 7 IPG strips showed that the total protein spots of Bartonella henselae Houston and Bartonella henselae Marseille were 375 and 379 respectively, 95% of the spots were the same between the two strains of Bartonella henselae.


CONCLUSIONS
The procedure of 2-DE may prove successful for the proteomic analysis of Bartonella henselae. Bartonella henselae Houston and Bartonella henselae Marseille are different genotypes.
Zhao
Su-Qing
SQ
Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France. suqingz@hotmail.com
Cai
Yan-Fei
YF
Zhu
Zhen-Yu
ZY
D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D018416:Bartonella henselae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005838:Genotype; D040901:Proteomics
D001426:Bacterial Proteins