Characterization of protein matrix motions in the Rb. sphaeroides photosynthetic reaction center.
We use Normal Mode Analysis to investigate motions in the photosynthetic reaction center (RC) protein. We identify the regions involved in concerted fluctuations of the protein matrix and analyze the normalized amplitudes and the directionality of the first few dominant modes. We also seek to quantify the coupling of normal modes to long-range electron transfer (ET). We find that a quasi-continuous spectrum of protein motions rather than one individual mode contributes to light-driven electron transfer. This is consistent with existing theoretical models (e.g. the spin-boson/dispersed polaron model) for the coupling of the protein and solvent "bath" to charge separation events. [Figure: see text].
Stoica
Ileana
I
National Research Council of Canada, 6100 Royalmount Ave., Montréal, H4P 2R2, Canada. ileana@bri.nrc.ca
D008958:Models, Molecular; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000378:metabolism*; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000201:enzymology*
D045322:Photosynthetic Reaction Center Complex Proteins; D021122:Protein Subunits