title: Relationship of vaginal cytology to alterations of the vaginal microflora of rats during the estrous cycle.
abstract: The influence of cyclic changes occurring in the vaginal tract during the estrous cycle upon the indigenous microflora of the vagina has been investigated by semiquantitative techniques in virgin female rats. By plate counts performed on material lavaged daily from the vaginal tract of several rats, it is apparent that bacterial counts are elevated in the proestrus and estrus phases of the cycle to values several orders of magnitude greater than those observed during metestrus or diestrus phases. Increases in vaginal bacterial counts were associated with the presence of cornified epithelial cells in the vagina; these cells were predominantly nonviable. Decreases in the vaginal bacterial content were related to the influx of leukocytes into the vagina after estrus. When leukocytes were present in the vaginal tract, they were 90 to 100% viable. From these observations it has been concluded that the female vaginal tract and the bacteria which colonize it represent a dynamic ecosystem which is responsive to cyclic events occurring in the estrous cycle. The changing cellular content of the vaginal tract may have relevance to the observed cyclic changes in the bacterial content of the vagina.
authors.affiliation: Department of Obstetrics and Gynecology, The University of Iowa, Iowa City, Iowa 52242.
authors.forename: B
authors.initials: B
authors.lastname: Larsen
authors.forename: A J
authors.initials: AJ
authors.lastname: Markovetz
authors.forename: R P
authors.initials: RP
authors.lastname: Galask