Effect of gramicidin on methanogenesis by various methanogenic bacteria.
Methanogenesis by Methanobacterium thermoautotrophicum strains was extremely sensitive to gramicidin, total inhibition being observed at 0.2 mug/ml. In contrast, methane synthesis by Methanococcus voltae, Methanogenium marisnigri, Methanosarcina mazei, and Methanospirillum hungatei were resistant to the highest concentrations of gramicidin tested (40 mug/ml), although spheroplasts of Methanospirillum hungatei were extremely sensitive. Other species tested showed intermediate sensitivity to gramicidin, methanogenesis inhibition occurring at 4 to 20 mug/ml.
Jarrell
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Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
Hamilton
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