Approach to novel functional foods for stress control 4. Regulation of serotonin transporter by food factors.
Serotonin transporters (SERTs) are pre-synaptic proteins specialized for the clearance of serotonin following vesicular release at central nervous system (CNS) and enteric nervous system synapses. SERTs are high affinity targets in vivo for antidepressants such as serotonin selective reuptake inhibitors (SSRIs). These include 'medical' psychopharmacological agents such as analgesics and antihistamines, a plant extract called St John's Wort (Hypericum). Osteoclasts are the primary cells responsible for bone resorption. They arise by the differentiation of osteoclast precursors of the monocyte/macrophage lineage. The expression of SERTs was increased in RANKL-induced osteoclast-like cells. Using RANKL stimulation of RAW264.7 cells as a model system for osteoclast differentiation, we studied the direct effects of food factor on serotonin uptake. The SSRIs (fluoxetine and fluvoxamine) inhibited markedly (approximately 95%) in serotonin transport in differentiated osteoclast cells. The major components of St. John's Wort, hyperforin and hypericine were significantly decreased in serotonin transport activity. Thus, a new in vitro model using RANKL-induced osteoclast-like cells may be useful to analyze the regulation of SERT by food factors and SSRIs.
Ito
Mikiko
M
Institute of Health Biosciences, The University of Tokushima Graduate School, Japan.
Haito
Sakiko
S
Furumoto
Mari
M
Kawai
Yoshichika
Y
Terao
Junji
J
Miyamoto
Ken-ichi
K
D000818:Animals; D000873:Anthracenes; D044946:Biflavonoids / Q000494:pharmacology; D001643:Bridged Bicyclo Compounds / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D002478:Cells, Cultured; D005473:Fluoxetine / Q000494:pharmacology; D016666:Fluvoxamine / Q000494:pharmacology; D005502:Food; D020902:Hypericum / Q000737:chemistry; D008024:Ligands; D008264:Macrophages / Q000166:cytology / Q000502:physiology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000378:metabolism*; D051379:Mice; D010010:Osteoclasts / Q000187:drug effects; D010569:Perylene / Q000031:analogs & derivatives / Q000494:pharmacology; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D011994:Recombinant Proteins / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism; D013312:Stress, Physiological / Q000517:prevention & control*; D013729:Terpenes / Q000494:pharmacology; D013997:Time Factors
D000873:Anthracenes; D044946:Biflavonoids; D001643:Bridged Bicyclo Compounds; D002352:Carrier Proteins; D008024:Ligands; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D010936:Plant Extracts; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D011994:Recombinant Proteins; D013729:Terpenes; C505687:Tnfrsf11a protein, mouse; C505689:Tnfsf11 protein, mouse; D005473:Fluoxetine; D012701:Serotonin; D010569:Perylene; C004965:hypericin; C011164:amentoflavone; D010696:Phloroglucinol; D016666:Fluvoxamine; C001654:hyperforin