Volumetric tomography of fluorescent proteins through small animals in vivo.
Volumetric detection and accurate quantification of fluorescent proteins in entire animals would greatly enhance our ability to monitor biological processes in vivo. Here we present a quantitative tomographic technique for visualization of superficial and deep-seated (>2-3 mm) fluorescent protein activity in vivo. We demonstrate noninvasive imaging of lung tumor progression in a murine model, as well as imaging of gene delivery using a herpes virus vector. This technology can significantly improve imaging capacity over the current state of the art and should find wide in vivo imaging applications in drug discovery, immunology, and cancer research.
Zacharakis
Giannis
G
Laboratory for Bio-optics and Molecular Imaging, Center for Molecular Imaging Research, Harvard Medical School, Charlestown, MA 02129, USA.
Kambara
Hirokazu
H
Shih
Helen
H
Ripoll
Jorge
J
Grimm
Jan
J
Saeki
Yoshinaga
Y
Weissleder
Ralph
R
Ntziachristos
Vasilis
V
D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis* / Q000235:genetics; D008175:Lung Neoplasms / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D051381:Rats; D018139:Simplexvirus / Q000235:genetics; D014054:Tomography / Q000379:methods*
D008164:Luminescent Proteins