title: Crystal structure and functional analysis of Dcp2p from Schizosaccharomyces pombe.
abstract: Decapping is a key step in both general and nonsense-mediated 5' --> 3' mRNA-decay pathways. Removal of the cap structure is catalyzed by the Dcp1-Dcp2 complex. The crystal structure of a C-terminally truncated Schizosaccharomyces pombe Dcp2p reveals two distinct domains: an all-helical N-terminal domain and a C-terminal domain that is a classic Nudix fold. The C-terminal domain of both Saccharomyces cerevisiae and S. pombe Dcp2p proteins is sufficient for decapping activity, although the N-terminal domain can affect the efficiency of Dcp2p function. The binding of Dcp2p to Dcp1p is mediated by a conserved surface on its N-terminal domain, and the N-terminal domain is required for Dcp1p to stimulate Dcp2p activity. The flexible nature of the N-terminal domain relative to the C-terminal domain suggests that Dcp1p binding to Dcp2p may regulate Dcp2p activity through conformational changes of the two domains.
authors.affiliation: Laboratory of Macromolecular Structure, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore.
authors.forename: Meipei
authors.initials: M
authors.lastname: She
authors.forename: Carolyn J
authors.initials: CJ
authors.lastname: Decker
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.forename: Suneeta
authors.initials: S
authors.lastname: Tumati
authors.forename: Roy
authors.initials: R
authors.lastname: Parker
authors.forename: Haiwei
authors.initials: H
authors.lastname: Song
mesh_terms: D002384:Catalysis; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011755:Pyrophosphatases / Q000737:chemistry / Q000378:metabolism; D012568:Schizosaccharomyces / Q000737:chemistry* / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D040681:Structural Homology, Protein
chemical_list: C508496:Dcp2 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D011755:Pyrophosphatases