A new hybrid electrospray Fourier transform mass spectrometer: design and performance characteristics.
A new hybrid electrospray quadrupole Fourier transform mass spectrometry (FTMS) instrument design is shown and characterized. This instrument involves coupling an electrospray source and mass-resolving quadrupole, ion accumulation, and collision cell linear ion trap system developed by MDS Sciex with a home-built ion guide and ion cyclotron resonance (ICR) cell. The iterative progression of this design is shown. The final design involves a set of hexapole ion guides to transfer the ions from the accumulation/collision trap through the magnetic field gradient and into the cell. These hexapole ion guides are separated by a thin gate valve and two conduction limits to maintain the required <10(-9) mbar vacuum for FTICR. Low-attomole detection limits for a pure peptide are shown, 220 000 resolving power in broadband mode and 820 000 resolving power in narrow-band mode are demonstrated, and mass accuracy in the <2 ppm range is routinely available provided the signal is abundant, cleanly resolved, and internally calibrated. This instrument design provides high experimental flexibility, allowing Q2 CAD, SORI-CAD, IRMPD, and ECD experiments with selected ion accumulation as well as experiments such as nozzle skimmer dissociation. Initial top-down mass spectrometry experiments on a protein is shown using ECD.
O'connor
Peter B
PB
Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 715 Albany St. R806, Boston, MA 02118-2526, USA.
Pittman
Jason L
JL
Thomson
Bruce A
BA
Budnik
Bogdan A
BA
Cournoyer
Jason C
JC
Jebanathirajah
Judith
J
Lin
Cheng
C
Moyer
Susanne
S
Zhao
Cheng
C
D002147:Calmodulin / Q000032:analysis* / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005583:Fourier Analysis; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D018511:Systems Integration
D002147:Calmodulin; D010455:Peptides