title: Immunization with dendritic cells retrovirally transduced with mycobacterial antigen 85A gene elicits the specific cellular immunity including cytotoxic T-lymphocyte activity specific to an epitope on antigen 85A.
abstract: In the present study, we evaluated antigen 85A (Ag85A) gene-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A gene and injected to BALB/c mice intravenously. The DC vaccine was capable of inducing purified protein derivative (PPD)- and the antigen-specific spleen cell proliferation and IFN-gamma production from both CD4+ and CD8+ T cells in spleens of the immune mice. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) and IFN-gamma-producing cells specific for a 9-mer CTL epitope on Ag85A molecule. This eliciting cellular immunity led to protection against wasting disease due to M. tuberculosis infection and induction of moderate bacterial clearance.
authors.affiliation: Second Division, Department of Internal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, and Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakano
authors.forename: Toshi
authors.initials: T
authors.lastname: Nagata
authors.forename: Takafumi
authors.initials: T
authors.lastname: Suda
authors.forename: Takao
authors.initials: T
authors.lastname: Tanaka
authors.forename: Taiki
authors.initials: T
authors.lastname: Aoshi
authors.forename: Masato
authors.initials: M
authors.lastname: Uchijima
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kuwayama
authors.forename: Noriko
authors.initials: N
authors.lastname: Kanamaru
authors.forename: Kingo
authors.initials: K
authors.lastname: Chida
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Nakamura
authors.forename: Masaji
authors.initials: M
authors.lastname: Okada
authors.forename: Yukio
authors.initials: Y
authors.lastname: Koide
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012190:Retroviridae / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014161:Transduction, Genetic
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D018984:Epitopes, T-Lymphocyte; C065036:antigen 85, Mycobacterium bovis