[Pathobiochemical importance of phospholipases for the release of mast cell mediators].
We investigated the influence of polyunsaturated fatty acids on the activity of the cytosolic phospholipase A2 (cPLA2) in the canine mastocytoma cell line C2 as a model for canine atopic dermatitis (CAD). Cells were cultured in a basic medium or in media supplemented with different fatty acids (14 microM) for eight days. The supplemented fatty acids were linoleic acid (18:2n6), alpha-linolenic acid (18:3n3), gamma-linolenic acid (18:3n6) and docosahexaenoic acid (22:6n3). We measured enriched concentrations of the added fatty acid, their delta6-desaturated and elongated products. However, delta5-desaturated products were not increased. Culturing of C2 in 18:3n3 supplemented medium reduced the cPLA2 activity. Furthermore in these cells and in C2 cultured in 22:6n3 supplemented medium decreased the cPLA2 activity after stimulation. The reduced cPLA2 activity by the changed fatty acid pattern of C2 cultured in 18:3n3 or 22:6n3 possibly explain the beneficial effects of these fatty acids in CAD because increased cPLA2 activity is accompanied by enhanced release of proinflammatory type 2 prostaglandins and type 4 leukotrienes.
Gück
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T
Veterinär-Physiologisch-Chemisches Institut, Veterinärmedizinische Fakultät, Universität Leipzig. gueck@vmf.uni-leipzig.de
Fuhrmann
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H
D000818:Animals; D045744:Cell Line, Tumor; D003876:Dermatitis, Atopic / Q000473:pathology / Q000662:veterinary*; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism*; D034801:Mastocytoma / Q000473:pathology / Q000662:veterinary; D010740:Phospholipases / Q000187:drug effects / Q000378:metabolism*
D005231:Fatty Acids, Unsaturated; D018836:Inflammation Mediators; D010740:Phospholipases