Magnetic resonance imaging of Klebsiella pneumoniae-induced pneumonia in mice.
In vivo imaging of small animals is a rapidly developing field. However, the potential of global imaging of infectious processes in animal models remains poorly explored. We used magnetic resonance imaging (MRI) to follow the development and regression of inflammatory lesions caused by infection by Klebsiella pneumoniae in mouse lungs. A virulent strain caused an intense inflammation within 2 days in the whole lungs, while an avirulent strain did not show significant changes. Mice infected with the virulent strain and subsequently treated with antibiotics presented a severe inflammation localized mainly in the left lung that disappeared after a week. The lesions observed by MRI correlated with the damage seen by histological analysis and a 3D representation of the tissue allowed better visualization of the development and healing of inflammatory lesions. MRI thus represents a powerful technique to study in vivo the interactions between a pathogen and its host in real time.
Tournebize
Régis
R
Pathogénie Microbienne Moléculaire - INSERM 389, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France. tournebi@pasteur.fr
Doan
Bich-Thuy
BT
Dillies
Marie-Agnès
MA
Maurin
Sabine
S
Beloeil
Jean-Claude
JC
Sansonetti
Philippe J
PJ
D000818:Animals; D005260:Female; D007711:Klebsiella pneumoniae / Q000472:pathogenicity*; D008168:Lung / Q000382:microbiology / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D051379:Mice; D008807:Mice, Inbred BALB C; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000473:pathology*