title: Lysed cell models and isolated chromosomes for the study of kinetochore/centromere biochemistry in vitro.
abstract: The centromere or kinetochore functions in both chromosome movement and in regulation of progression through mitosis. It appears likely that the signaling pathways involved are keenly dependent on solid phase cytoskeletal and karyoskeletal scaffolds that may mediate important physical signals such as tension. Understanding these pathways will be greatly aided by reconstructing the signaling in lysed cell models. Here we present approaches to the in vitro study of signaling pathways in mitotic cells, particularly those involved in protein phosphorylation changes at kinetochores that may control cell cycle progression in M phase.
authors.affiliation: Program in Molecular, Cell and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Daum
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Gorbsky
mesh_terms: D000818:Animals; D002460:Cell Line; D002503:Centromere / Q000737:chemistry / Q000201:enzymology; D033481:Chromosomes, Mammalian / Q000201:enzymology; D003582:Cytogenetics / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D018386:Kinetochores / Q000737:chemistry / Q000201:enzymology*; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000502:physiology; D008954:Models, Biological; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D048328:Potoroidae; D015398:Signal Transduction
chemical_list: D010770:Phosphotransferases; D010744:Phosphoric Monoester Hydrolases