On the surface modification of microchannels for microcapillary electrophoresis chips.
This paper presents systematic investigation of the microchannel surface properties in microCE chips. Three popular materials for microCE chips, polydimethylsiloxane (PDMS), quartz, and glass, are used. The zeta potentials of these microchannels are calculated by measuring the EOF velocity to evaluate the surface properties after surface modification. The hydrophobic PDMS is usually plasma-treated for microCE applications. In this study, a new method using a high-throughput atmospheric plasma generator is adopted to treat the PDMS surface under atmospheric conditions. In this approach, the cost and time for surface treatment can be significantly reduced compared with the conventional vacuum plasma generator method. Experimental results indicate that new functional groups could be formed on the PDMS surface after treatment, resulting in a change in the surface property. The time-dependent surface property of the plasma-treated PDMS is then measured in terms of the zeta potential. Results show that the surface property will reach a stable condition after 1 h of plasma treatment. For glass CE chips, two new methods for changing the microchannel surface properties are developed. Instead of using complicated and time-consuming chemical silanization procedures for CE channel surface modification, two simple and reliable methods utilizing organic-based spin-on-glass and water-soluble acrylic resin are reported. The proposed method provides a fast batch process for controlling the surface properties of glass-based CE channels. The proposed methods are evaluated using PhiX-174 DNA maker separation. The experimental data show that the surface property is modified and separation efficiency greatly improved. In addition, the long-term stability of both coatings is verified in this study. The methods proposed in this study show potential as an excellent solution for glass-based microCE chip surface modification.
Lee
Gwo-Bin
GB
Department of Engineering Science, National Cheng Kung University, Tainan, Taiwan. gwobin@mail.ncku.edu.tw
Lin
Che-Hsin
CH
Lee
Kuo-Hoong
KH
Lin
Yue-Feng
YF
D010584:Bacteriophage phi X 174 / Q000235:genetics; D004279:DNA, Viral / Q000302:isolation & purification; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D005898:Glass / Q000737:chemistry; D006851:Hydrochloric Acid / Q000737:chemistry; D009995:Osmosis; D011791:Quartz / Q000737:chemistry; D012828:Silicones / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties
D004279:DNA, Viral; D004129:Dimethylpolysiloxanes; D012828:Silicones; D011791:Quartz; D012972:Sodium Hydroxide; C013830:baysilon; D006851:Hydrochloric Acid