title: [Stability and in vitro activity of voriconazole eyedrops at a concentration of 3 microg/mL].
abstract: OBJECTIVE
  To assess the stability and activity of voriconazole 3 microg/mL eyedrops as prepared for use against amphotericin B- and fluconazole-resistant fungal endophthalmitis.
  
  
  METHODS
  Stability (concentration using UV-spectrophotometry; pH, osmolarity, and particle formation) and sterility were analyzed under various preservation conditions--room temperature (22-24 degrees C) or refrigerated (2-8 degrees C). The preparation's in vitro efficacy was analyzed using the standard National Committee for Clinical Laboratory Standards method for 30 days.
  
  
  RESULTS
  Voriconazole concentrations were found to be within limits allowed by the United Stated Pharmacopeia (90-115%). pH (room temperature: 6.96-7.60; refrigerated: 6.84-7.21) and osmolarity (room temperature: 265-284 mOsm/l; refrigerated: 270-285 mOsm/l) remained within eye physiological ranges throughout the study under the analyzed conditions. The preparation s antifungal activity remained stable during the first three weeks.
  
  
  CONCLUSIONS
  The voriconazole 3 microg/mL eyewash preparation remained stable, sterile and with full antifungal activity for 21 days when stored both at room temperature and under refrigeration conditions.
authors.affiliation: Servicio de Farmacia Hospitalaria, Hospital Universitario Reina Sofía, Córdoba. beatrizislatj@hotmail.com
authors.forename: B
authors.initials: B
authors.lastname: Isla Tejera
authors.forename: C
authors.initials: C
authors.lastname: Garzas Martín de Almagro
authors.forename: M
authors.initials: M
authors.lastname: Cárdenas Aranzana
authors.forename: I
authors.initials: I
authors.lastname: Pérez Rodrigo
authors.forename: M D
authors.initials: MD
authors.lastname: Aumente Rubio
authors.forename: R
authors.initials: R
authors.lastname: Gordillo Sánchez
mesh_terms: D000935:Antifungal Agents / Q000008:administration & dosage* / Q000494:pharmacology; D004355:Drug Stability; D009883:Ophthalmic Solutions; D011743:Pyrimidines / Q000008:administration & dosage* / Q000494:pharmacology; D014230:Triazoles / Q000008:administration & dosage* / Q000494:pharmacology; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D009883:Ophthalmic Solutions; D011743:Pyrimidines; D014230:Triazoles; D065819:Voriconazole