Induction of a cytosolic pyruvate kinase 1 gene during the resistance response to Tobacco mosaic virus in Capsicum annuum.
Hot pepper (Capsicum annuum L. cv. Bugang) plants exhibit a hypersensitive response (HR) upon infection by Tobacco mosaic virus (TMV) pathotype P(0). Previously, to elucidate molecular mechanism that underlies this resistance, hot pepper cv. Bugang leaves were inoculated with TMV-P(0) and genes specifically up-regulated during the HR were isolated by microarray analysis. One of the clones, Capsicum annuum cytosolic pyruvate kinase 1 (CaPK(c)1) gene was increased specifically in the incompatible interaction with TMV-P(0). The expression of CaPK(c)1 gene was also triggered not only by various hormones such as salicylic acid (SA), ethylene, and methyl jasmonate (MeJA), but also NaCl and wounding. These results suggest that CaPK(c)1 responds to several defense-related abiotic stresses in addition to TMV infection.
Kim
Ki-Jeong
KJ
School of Life Sciences and Biotechnology, Korea University, 1, 5-ga, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea.
Park
Chang-Jin
CJ
Ham
Byung-Kook
BK
Choi
Soo Bok
SB
Lee
Boo-Ja
BJ
Paek
Kyung-Hee
KH
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002212:Capsicum / Q000201:enzymology* / Q000235:genetics / Q000821:virology*; D004790:Enzyme Induction; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000201:enzymology; D021381:Protein Transport; D011770:Pyruvate Kinase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D014027:Tobacco Mosaic Virus / Q000502:physiology*
D001426:Bacterial Proteins; D011770:Pyruvate Kinase