Stable defined substrate for turbidimetric assay of endoxylanases.
A stable xylan suspension was prepared and characterized. Hydrolysis of the particles converts them into soluble fragments, thereby lowering the turbidity of the suspension. The small volume of the assay mixture, the short incubation time required, and the simplicity of the procedure permit the rapid analysis of many samples. Furthermore, the procedure can be used to assay xylanase activities in the presence of other reducing materials and is also useful for monitoring low-level xylanase activities.
Wang
P
P
Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, Manchester M60 1QD, United Kingdom.
Broda
P
P