title: Effect of sucrose concentration on dental biofilm formed in situ and on enamel demineralization.
abstract: The relationship between sucrose concentration and cariogenic potential was studied in situ. Adult volunteers wore intraoral palatal appliances containing human dental enamel blocks, which were extraorally submitted 8 times a day for 14 days, to the treatments: deionized distilled water and sucrose solutions from 1 to 40%. The biofilm formed was analyzed with respect to acidogenicity and biochemical composition; enamel demineralization was evaluated by microhardness. The results showed that 1% sucrose is less cariogenic than 5% or higher concentrations, although sucrose solution at 40% was still able to increase the concentration of insoluble polysaccharide in the biofilm formed. The findings suggest that the threshold of sucrose solution concentration for the formation of a cariogenic biofilm is 5%, which provided the same cariogenic potential as that observed for 10 and 20% sucrose solution.
authors.affiliation: Faculty of Dentistry of Piracicaba, UNICAMP, Piracicaba, Brazil.
authors.forename: C P
authors.initials: CP
authors.lastname: Aires
authors.forename: C P M
authors.initials: CP
authors.lastname: Tabchoury
authors.forename: A A
authors.initials: AA
authors.lastname: Del Bel Cury
authors.forename: H
authors.initials: H
authors.lastname: Koo
authors.forename: J A
authors.initials: JA
authors.lastname: Cury
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D018441:Biofilms / Q000254:growth & development*; D002326:Cariogenic Agents / Q000008:administration & dosage*; D003743:Dental Enamel / Q000737:chemistry / Q000187:drug effects; D003773:Dental Plaque / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006244:Hardness / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016037:Single-Blind Method; D013395:Sucrose / Q000008:administration & dosage*; D017001:Tooth Demineralization / Q000139:chemically induced*
chemical_list: D002326:Cariogenic Agents; D013395:Sucrose