Study of isothermal adsorption of albumin on acetate fiber filter rod with dye affinity ligand.
Cibacron Blue F3GA was immobilized onto an acetate fiber filter rod to produce potential affinity matrix. The adsorption isotherms of the prepared matrix for either bovine serum albumin or human serum albumin were investigated. It was experimentally shown that the affinity isothermal adsorption obeyed Freundlich adsorption model. By means of affinity chromatography of human plasma using the prepared matrix, purified human serum albumin was obtained.
Li
Jing-Hua
JH
Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China.
Wang
Jun-De
JD
Liu
Xue-Liang
XL
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000302:isolation & purification
D008024:Ligands; D012709:Serum Albumin