Method for adenosine 5'-triphosphate measurement on coke waste activated sludge.
Measurement of adenosine 5'-triphosphate (ATP) in coke waste activated sludge can provide a simple method for estimating the levels of viable microbes in the sludge. However, the presence of inhibitors such as phenol in the sludge interferes when the luciferin-luciferase method is used to measure ATP. These inhibiting substances can be removed from the sludge before extraction of ATP by washing the cells with dilute sodium dodecyl sulfate.
Russell
J
J
Department of Biology, University of Alabama in Birmingham, Birmingham, Alabama 35294.
Gauthier
J J
JJ