title: Oxidant and carbonyl stress-related apoptosis in end-stage kidney disease: impact of membrane flux.
abstract: Apoptosis is a highly regulated process which mostly affects cell-mediated immunity. In this open-label, randomized, prospective clinical study, we determined the impact in 10 hemodialysis patients treated with high-, medium-, and low-flux membranes on spontaneous or plasma-induced apoptosis, on monocytes, as well as on oxidant and carbonyl stress. High- and medium-flux membranes significantly reduced patients' plasma-dependent proapoptotic activity on U937 monocytic cell lines. Patients who had the highest levels of plasma-induced proapoptotic activity exhibited the highest plasma levels of advanced oxidation protein products (AOPPs) and carbonyls. Plasma carbonyl residues but not AOPPs were significantly lowered. Finally, a significant correlation could be drawn between the extent of plasma-induced proapoptotic activity and both plasma carbonyl and AOPP levels.
authors.affiliation: Department of Nephrology, San Bortolo Hospital, Vicenza, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Bordoni
authors.forename: Marta
authors.initials: M
authors.lastname: Piroddi
authors.forename: Francesco
authors.initials: F
authors.lastname: Galli
authors.forename: Massimo
authors.initials: M
authors.lastname: de Cal
authors.forename: Monica
authors.initials: M
authors.lastname: Bonello
authors.forename: Petras
authors.initials: P
authors.lastname: Dimitri
authors.forename: Gabriella
authors.initials: G
authors.lastname: Salvatori
authors.forename: Rattanarat
authors.initials: R
authors.lastname: Ranishta
authors.forename: Nathan
authors.initials: N
authors.lastname: Levin
authors.forename: Ciro
authors.initials: C
authors.lastname: Tetta
authors.forename: Claudio
authors.initials: C
authors.lastname: Ronco
mesh_terms: D000328:Adult; D017209:Apoptosis; D001798:Blood Proteins / Q000032:analysis; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009000:Monocytes; D016877:Oxidants / Q000097:blood; D010084:Oxidation-Reduction; D006435:Renal Dialysis; D020298:U937 Cells
chemical_list: D001798:Blood Proteins; D008567:Membranes, Artificial; D016877:Oxidants