Comparison of several methods for the extraction of DNA from potatoes and potato-derived products.
Eight methods were compared for the extraction of DNA from raw potato tubers, and nine methods were evaluated for the extraction of DNA from dehydrated potato slices, potato flakes, potato flour, potato starch, and two ready-to-eat potato snack foods. Extracts were assessed for yield using a fluorescence-based DNA quantification assay. Real-time amplification of an endogenous gene, sucrose synthase (sus), was used to assess extract and template quality. A CTAB-based method extracted the highest DNA yields from the tuber material. An in-house method, which utilized the Kingfisher magnetic particle processor, yielded the highest template quality from the tubers. For most of the tuber samples, the Kingfisher and CTAB methods recovered the highest levels of amplifiable sus. DNA yields for potato-derived foods generally decreased with the extent that the product had been processed. The methods that utilized the magnetic particle processor delivered the highest template quality from one of the snack products that was particularly high in fat. For most of the remaining processed products, the levels of amplifiable target DNA recovered were roughly correlated with total DNA recovery, indicating that overall yield had greater influence over sus amplification than template quality. The Wizard method was generally the best method for the extraction of DNA from most of the potato-derived foods.
Smith
Donna S
DS
Charlottetown Laboratory, Canadian Food Inspection Agency, 93 Mount Edward Road, Charlottetown, Prince Edward Island C1A 5T1, Canada. smithds@inspection.gc.ca
Maxwell
Philip W
PW
De Boer
Solke H
SH
D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D018744:DNA, Plant / Q000302:isolation & purification*; D004586:Electrophoresis; D004587:Electrophoresis, Agar Gel; D005433:Flour / Q000032:analysis; D005511:Food Handling; D018745:Genome, Plant; D007202:Indicators and Reagents; D018517:Plant Roots / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000737:chemistry*; D013213:Starch / Q000737:chemistry
D002593:Cetrimonium Compounds; D018744:DNA, Plant; D007202:Indicators and Reagents; D013213:Starch; D000077286:Cetrimonium