title: Soybean isoflavones alter parvalbumin in hippocampus of mid-aged normal female, ovariectomized female, and normal male rats.
abstract: OBJECTIVE
  To investigate the long-term effect of soybean isoflavones on changes in parvalbumin (PV) immunoreactivity in the hippocampus in normal female, ovariectomized (OVX) female and normal male rats.
  
  
  METHODS
  Ten-month-old rats were assigned to one of 9 groups (n = 7 in each group) based on body weight using a randomized complete-block design. The groups were: control diet-treated females, OVX females, and males; 0.3 g/kg isoflavone-treated females, OVX females, and males; and 1.2 g/kg isoflavone-treated females, OVX females, and males. The PV immunostaining was conducted by using the standard avidin-biotin complex method.
  
  
  RESULTS
  PV immunoreactivity and the number of PV-immunoreactive neurons in all the groups after isoflavone treatment were significantly changed in the hippocampal CA1 region and in the dentate gyrus, but not in the hippocampal CA2/3 region. PV immunoreactivity and the number of PV-immunoreactive neurons in the control diet OVX females were similar to those in the control diet, and were greater than those in the control diet normal females. PV immunoreactivity and the number of PV-immunoreactive neurons in all the isoflavone-treated groups decreased dose-dependently after isoflavone treatment.
  
  
  CONCLUSIONS
  Long-term administration of isoflavones may induce a reduction of PV in interneurons in the hippocampal CA1 region and in the dentate gyrus. The reduction of PV in these regions suggests that the long-term administration of isoflavones may cause a change in calcium homeostasis in the hippocampal CA1 region and in the dentate gyrus.
authors.affiliation: Department of Anatomy, College of Medicine, Hallym University, Chunchon, Korea.
authors.forename: In-koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Yoon-bok
authors.initials: YB
authors.lastname: Lee
authors.forename: Ki-yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Tae-cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Soon-sung
authors.initials: SS
authors.lastname: Lim
authors.forename: Sang-moo
authors.initials: SM
authors.lastname: Kim
authors.forename: Heon-soo
authors.initials: HS
authors.lastname: Sohn
authors.forename: Woo-jung
authors.initials: WJ
authors.lastname: Kim
authors.forename: Hyun-kyung
authors.initials: HK
authors.lastname: Shin
authors.forename: Moo-ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000378:metabolism; D005260:Female; D019833:Genistein / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism*; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000378:metabolism; D010052:Ovariectomy; D010320:Parvalbumins / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max / Q000737:chemistry
chemical_list: D007529:Isoflavones; D010320:Parvalbumins; C004742:daidzein; C086566:glycitein; D019833:Genistein