Low temperatures during early development influence subsequent maternal and reproductive function in adult female mice.
Challenging conditions early in development can have enduring effects into adulthood. The effects of low temperatures were examined on subsequent sex-specific morphology (anogenital distance [AGD]), maternal care, and reproductive performance in adult female mice. Dams (F0) were maintained either in (1) standard laboratory room temperatures (21 +/- 2 degrees C) or (2) low temperatures (10 +/- 2 degrees C) throughout gestation. Their progeny (F1) either remained in the temperature condition in which they were conceived or were switched to the other temperature condition at 2 days of age until weaning. Reproductive performance and maternal behaviors were assessed in adulthood. F0 dams that were maintained in low temperatures bore larger litters as compared to F0 animals housed in standard temperatures throughout their pregnancy. In contrast, mean litter size was reduced for all groups of F1 females that experienced low temperatures. Infant mortality was elevated in litters of F1 females that were exposed to low temperatures both before and after birth. Prenatal exposure to low temperatures was associated with reduced responsiveness towards the nursing young and decreased maternal aggression in F1 animals. Prenatally treated F1 females had longer, male-like AGDs on Day 2 following birth compared to animals not subjected to experimental manipulations. Our results indicate that exposure to low temperatures during early development impairs reproductive function and is associated with important fitness costs as evidenced by reduced offspring survival. Our findings also suggest that chronic low temperatures experienced only after birth may have less deleterious effects than exposure to a combination of pre- and postnatal or prenatal treatments alone.
Benderlioglu
Zeynep
Z
Departments of Psychology and Neuroscience, Institute for Behavioral Medicine Research, 09 Townshend Hall, Ohio State University, Columbus, OH 43210, USA.
Eish
Jenny
J
Weil
Zachary M
ZM
Nelson
Randy J
RJ
D000374:Aggression / Q000502:physiology; D000818:Animals; D001724:Birth Weight / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D003080:Cold Temperature / Q000009:adverse effects*; D005260:Female; D005836:Genitalia, Female / Q000254:growth & development; D008098:Litter Size / Q000502:physiology; D008425:Maternal Behavior / Q000502:physiology*; D051379:Mice; D010641:Phenotype; D012098:Reproduction / Q000502:physiology*; D013534:Survival