title: Relationship between Methanogenic Cofactor Content and Maximum Specific Methanogenic Activity of Anaerobic Granular Sludges.
abstract: In this study we investigated whether a relationship exists between the methanogenic activity and the content of specific methanogenic cofactors of granular sludges cultured on different combinations of volatile fatty acids in upflow anaerobic sludge blanket or fluidized-bed reactors. Significant correlations were measured in both cases between the contents of coenzyme F(420)-2 or methanopterin and the maximum specific methanogenic activities on propionate, butyrate, and hydrogen, but not acetate. For both sludges the content of sarcinapterin appeared to be correlated with methanogenic activities on propionate, butyrate, and acetate, but not hydrogen. Similar correlations were measured with regard to the total content of coenzyme F(420)-4 and F(420)-5 in sludges from fluidized-bed reactors. The results indicate that the contents of specific methanogenic cofactors measured in anaerobic granular sludges can be used to estimate the hydrogenotrophic or acetotrophic methanogenic potential of these sludges.
authors.affiliation: Department of Microbiology, Faculty of Science, University of Nijmegen, NL-6525 ED Nijmegen, The Netherlands.
authors.forename: L G
authors.initials: LG
authors.lastname: Gorris
authors.forename: T M
authors.initials: TM
authors.lastname: de Kok
authors.forename: B M
authors.initials: BM
authors.lastname: Kroon
authors.forename: C
authors.initials: C
authors.lastname: van der Drift
authors.forename: G D
authors.initials: GD
authors.lastname: Vogels