[Transient expression of foreign ble gene in Dunaliella salina].
Plasmid pSP124S with ble gene was transformed into D. salina cell by electroporation. The retention and expression of foreign gene in D.salina was explored, proper parameters were established. A large amount of foreign plasmid was found to be introduced into cells. The plasmid was degraded gradually, but can be detected within 96 hours. The ble gene was efficiently transcribed from foreign promoter and transcription was maintained at least 72 hours. The ble gene can be translated correctly and so can be used as a selectable marker for the research of genetic tranformation in D. salina.
Li
Zi Xing
ZX
School of Life Sciences, Shanghai University, Shanghai.
Sun
Yu
Y
Liu
Zhi Xue
ZX
Song
Ren Tao
RT
Xu
Zheng Kai
ZK
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D000460:Chlorophyta / Q000235:genetics / Q000378:metabolism*; D018274:Electroporation; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001426:Bacterial Proteins