Identification of allergens in Indian fishes: hilsa and pomfret exemplified by ELISA and immunoblotting.
Enzymed-linked immunosorbent assay of hilsa and pomfret muscle extracts showed specific IgE binding to ten allergic patients' sera, the results corroborated to that of skin prick test. Comparison of allergen profiles of the two fish extracts by immunoblotting revealed a common antigenic protein of 50 kDa and some high molecular weight fish allergens instead of low molecular weight parvalbumin found in several fishes. Purified and well characterized fish allergens are always considered better than crude fish extracts for diagnostic use.
Das
Arpita
A
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700 032, India.
Chakraborti
Phuljhuri
P
Chatterjee
Urmimala
U
Monddal
Gautam
G
Chatterjee
Bishnu P
BP
D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005399:Fishes / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000097:blood; D008297:Male; D008875:Middle Aged; D012882:Skin Tests
D000485:Allergens; D007073:Immunoglobulin E