Transfer of Plasmids to an Antibiotic-Sensitive Mutant of Zymomonas mobilis.
Wild-type strains of Zymomonas mobilis exhibit multiple antibiotic resistance and thus restrict the use of many broad-host-range plasmids in them as cloning vehicles. Antibiotic-sensitive mutants of Z. mobilis were isolated and used as hosts for the conjugal transfer of broad-host-range plasmids from Escherichia coli. Such antibiotic-sensitive strains can facilitate the application of broad-host-range plasmids to the study of Z. mobilis.
Buchholz
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Department of Biochemistry and Microbiology, Cook College, Rutgers University, New Brunswick, New Jersey 08903.
Eveleigh
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