The core centromere and Sgo1 establish a 50-kb cohesin-protected domain around centromeres during meiosis I.
The stepwise loss of cohesins, the complexes that hold sister chromatids together, is required for faithful meiotic chromosome segregation. Cohesins are removed from chromosome arms during meiosis I but are maintained around centromeres until meiosis II. Here we show that Sgo1, a protein required for protecting centromeric cohesins from removal during meiosis I, localizes to cohesin-associated regions (CARs) at the centromere and the 50-kb region surrounding it. Establishment of this Sgo1-binding domain requires the 120-base-pair (bp) core centromere, the kinetochore component Bub1, and the meiosis-specific factor Spo13. Interestingly, cohesins and the kinetochore proteins Iml3 and Chl4 are necessary for Sgo1 to associate with pericentric regions but less so for Sgo1 to associate with the core centromeric regions. Finally, we show that the 50-kb Sgo1-binding domain is the chromosomal region where cohesins are protected from removal during meiosis I. Our results identify the portions of chromosomes where cohesins are protected from removal during meiosis I and show that kinetochore components and cohesins themselves are required to establish this cohesin protective domain.
Kiburz
Brendan M
BM
Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, 02139, USA.
Reynolds
David B
DB
Megee
Paul C
PC
Marston
Adele L
AL
Lee
Brian H
BH
Lee
Tong Ihn
TI
Levine
Stuart S
SS
Young
Richard A
RA
Amon
Angelika
A
D018797:Cell Cycle Proteins / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone; D015825:Chromosomes, Fungal / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D018386:Kinetochores / Q000378:metabolism*; D049469:Meiotic Prophase I / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D000097722:Cohesins
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; C081874:CHL4 protein, S cerevisiae; C477821:IML3 protein, S cerevisiae; C482640:Sgo1 protein, S cerevisiae