Spatio-temporal distributions of metal ions and Taxol of Taxus cuspidata cells immobilized on polyurethane foam.
Distinct spatio-temporal variations of metal ions and Taxol production were observed for Taxus cuspidata cells immobilized on polyurethane foam. The Taxol content in the inner foam layer reached 215 microg g(-1) at day 30, which was 40-fold higher than that in the outer foam layer, and the Ca2+ and Mg2+ contents were 5.3 and 3.7 times higher, while the K+ content was 5.5 times lower. Thus higher intracellular Ca2+ and Mg2+ contents and lower intracellular K+ content may favor the Taxol biosynthesis in immobilized Taxus cuspidata.
Yin
De-Ming
DM
Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P. O. Box 6888, Tianjin, 300072, PR China.
Wu
Jin-Chuan
JC
Yuan
Ying-Jin
YJ
D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000378:metabolism; D018533:Biomass; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D002460:Cell Line; D018914:Cells, Immobilized / Q000378:metabolism; D008274:Magnesium / Q000032:analysis* / Q000378:metabolism; D017239:Paclitaxel / Q000032:analysis* / Q000378:metabolism; D011140:Polyurethanes; D011188:Potassium / Q000032:analysis* / Q000378:metabolism; D020946:Taxus / Q000166:cytology / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D011140:Polyurethanes; D008274:Magnesium; D017239:Paclitaxel; D011188:Potassium; D002118:Calcium