Properties of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) films modified with polyvinylpyrrolidone and behavior of MC3T3-E1 osteoblasts cultured on the blended films.
A series of composite films of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) modified with polyvinylpyrrolidone (PVP) was prepared by varying the ratio of constituents, and their properties and cytocompatibility were evaluated. The hydrophilicity of the blended materials surfaces increased and the amounts of fibronectin and laminin adsorbed on the materials surface increased remarkably compared with PHBHHx. FT-IR spectra of the blended films showed a new band, implying that a surface physical interpenetrating network structure had formed. Scanning electron microscopy showed that there were dense pits and holes on the blended films surface. For the films of PHBHHx with 20 wt% and 40 wt% PVP, MTT assay indicated that PVP enhanced cell adhesion and proliferation, but that the effects were impaired by excessive PVP. The results suggested that proper addition of PVP increased the cytocompatibility of PHBHHx because the material surface had increased hydrophilicity and presented an appropriate morphology.
Xi
Jing
J
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, P.R. China.
Kong
Lijun
L
Gao
Yuan
Y
Gong
Yandao
Y
Zhao
Nanming
N
Zhang
Xiufang
X
D020155:3-Hydroxybutyric Acid / Q000737:chemistry*; D016475:3T3 Cells; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D002208:Caproates / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry; D006885:Hydroxybutyrates / Q000737:chemistry; D007797:Laminin / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D008968:Molecular Conformation; D010006:Osteoblasts / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011205:Povidone / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D023822:Tissue Engineering; D000094122:Polyhydroxybutyrates
D020155:3-Hydroxybutyric Acid; D001672:Biocompatible Materials; D002208:Caproates; D004396:Coloring Agents; D005353:Fibronectins; D006885:Hydroxybutyrates; D007797:Laminin; D011091:Polyesters; D011108:Polymers; D011205:Povidone; D013778:Tetrazolium Salts; D013844:Thiazoles; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C022616:thiazolyl blue