Influence of the physiologically widespread substances on the oxidative activity of copper(II) complexes with sinefungin, a nucleoside antibiotic.
The oxidation-promoting reactivity of the Cu(II)-sinefungin complex in the presence of hydrogen peroxide was studied at pH 7.4, using N,N-dimethyl-p-nitrosoaniline (NDMA), as well as plasmid DNA as target molecules. Mixture of the complex with H(2)O(2) was found to be an efficient oxidant, bleaching NDMA solution, and generating single- and double-strand breaks in DNA. The oxidative DNA damage was investigated also in the presence of varying amounts of glutathione, histidine, Gly-Gly-His peptide, H2A histone, and ascorbic acid, showing diverse influence of those substances on the cleavage extension.
Mucha
Ariel
A
Faculty of Chemistry, University of Wrocław, F. Joliot-Curie 14, 50383 Wrocław, Poland.
Cappanelli
Massimo
M
Szczepanik
Wojciech
W
Kaczmarek
Piotr
P
Skała
Jacek
J
Jezowska-Bojczuk
Małgorzata
M
D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism
D000900:Anti-Bacterial Agents; D009942:Organometallic Compounds; D017382:Reactive Oxygen Species; D003300:Copper; D004247:DNA; D000241:Adenosine; C006235:sinefungin