Monitoring of microenvironmental changes in the major and minor grooves of DNA by dan-modified oligonucleotides.
[graph: see text] We describe the synthesis of new environmentally sensitive fluorescence probes to elucidate DNA structures. DNA oligonucleotides containing fluorophore dan (6-(dimethylamino)-2-acylnaphthalene)-modified dC or dG were able to monitor the microenvironmental changes in both the major and minor grooves of DNA with a B- to A-DNA conformational transition and RNA hybridization.
Kimura
Takumi
T
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
Kawai
Kiyohiko
K
Majima
Tetsuro
T
D004247:DNA / Q000737:chemistry*; D015335:Molecular Probes / Q000138:chemical synthesis*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D011066:Poly C; D011068:Poly G; D013050:Spectrometry, Fluorescence
D015335:Molecular Probes; D009841:Oligonucleotides; D011068:Poly G; C023826:poly(dC); C026628:poly(dG); D011066:Poly C; D004247:DNA