Oxidant and carbonyl stress-related apoptosis in end-stage kidney disease: impact of membrane flux.
Apoptosis is a highly regulated process which mostly affects cell-mediated immunity. In this open-label, randomized, prospective clinical study, we determined the impact in 10 hemodialysis patients treated with high-, medium-, and low-flux membranes on spontaneous or plasma-induced apoptosis, on monocytes, as well as on oxidant and carbonyl stress. High- and medium-flux membranes significantly reduced patients' plasma-dependent proapoptotic activity on U937 monocytic cell lines. Patients who had the highest levels of plasma-induced proapoptotic activity exhibited the highest plasma levels of advanced oxidation protein products (AOPPs) and carbonyls. Plasma carbonyl residues but not AOPPs were significantly lowered. Finally, a significant correlation could be drawn between the extent of plasma-induced proapoptotic activity and both plasma carbonyl and AOPP levels.
Bordoni
Valeria
V
Department of Nephrology, San Bortolo Hospital, Vicenza, Italy.
Piroddi
Marta
M
Galli
Francesco
F
de Cal
Massimo
M
Bonello
Monica
M
Dimitri
Petras
P
Salvatori
Gabriella
G
Ranishta
Rattanarat
R
Levin
Nathan
N
Tetta
Ciro
C
Ronco
Claudio
C
D000328:Adult; D017209:Apoptosis; D001798:Blood Proteins / Q000032:analysis; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009000:Monocytes; D016877:Oxidants / Q000097:blood; D010084:Oxidation-Reduction; D006435:Renal Dialysis; D020298:U937 Cells
D001798:Blood Proteins; D008567:Membranes, Artificial; D016877:Oxidants