title: Postprandial lipemic response and lipoprotein composition in subjects with low or high cholesterol absorption efficiency.
abstract: BACKGROUND
  The purpose of this study was to investigate the effect of differences in cholesterol absorption efficiency on the postprandial lipemia and lipoprotein composition.
  
  
  METHODS
  Fifteen healthy subjects were divided into low and high cholesterol absorbers on the basis of serum cholestanol to cholesterol ratio. A high-performance liquid chromatographic method with evaporative light scattering detection was developed for quantitation of free and esterified cholesterol, triglycerides and major phospholipids from the same lipid extract in two runs utilizing the same internal standard.
  
  
  RESULTS
  The free cholesterol to phosphatidylcholine ratio of chylomicrons was higher in the high cholesterol absorption group. The total increase of cholesterol in combined chylomicron and very low density lipoprotein (VLDL) fraction was also higher in this group. Chylomicron free cholesterol and cholesterol ester responses correlated with fasting low density lipoprotein (LDL) cholesterol. VLDL and VLDL1 triglyceride responses correlated inversely with fasting insulin and homeostasis model assessment of insulin resistance.
  
  
  CONCLUSIONS
  High cholesterol absorption efficiency was seen in chylomicrons as higher cholesterol to phosphatidylcholine ratio during the postprandial peak. Chylomicron cholesterol response was linked to fasting LDL cholesterol and low VLDL triglyceride response to fasting insulin.
authors.affiliation: Department of Physiology, University of Kuopio, P.O.B. 1627, SF-70211 Kuopio, Finland. Jyrki.Agren@uku.fi
authors.forename: Jyrki J
authors.initials: JJ
authors.lastname: Agren
authors.forename: Maarit
authors.initials: M
authors.lastname: Hallikainen
authors.forename: Helvi
authors.initials: H
authors.lastname: Vidgren
authors.forename: Tatu A
authors.initials: TA
authors.lastname: Miettinen
authors.forename: Helena
authors.initials: H
authors.lastname: Gylling
mesh_terms: D000328:Adult; D000368:Aged; D004083:Cholestanol / Q000097:blood; D002784:Cholesterol / Q000097:blood*; D002788:Cholesterol Esters / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D002914:Chylomicrons / Q000097:blood / Q000737:chemistry; D004041:Dietary Fats / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005215:Fasting / Q000097:blood; D006801:Humans; D007408:Intestinal Absorption; D008055:Lipids / Q000097:blood / Q000737:chemistry; D008074:Lipoproteins / Q000097:blood* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000097:blood; D010716:Phosphatidylinositols / Q000097:blood; D019518:Postprandial Period; D013109:Sphingomyelins / Q000097:blood; D014280:Triglycerides / Q000097:blood
chemical_list: D002788:Cholesterol Esters; D015243:Cholesterol, VLDL; D002914:Chylomicrons; D004041:Dietary Fats; D008055:Lipids; D008074:Lipoproteins; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D013109:Sphingomyelins; D014280:Triglycerides; D004083:Cholestanol; D002784:Cholesterol