title: Mitochondrial targeting of growth suppressor protein DLC2 through the START domain.
abstract: Deleted in liver cancer 2 (DLC2) is a candidate tumor suppressor frequently found to be deleted in hepatocellular carcinoma. In this study, we determined the subcellular localization of DLC2. Co-localization and biochemical fractionation studies revealed that DLC2 localized to mitochondria. In addition, the DLC2-containing cytoplasmic speckles were in proximity to lipid droplets. A DLC2 mutant containing the steroidogenic acute regulatory protein-related lipid transfer (START) domain only showed a localization pattern identical to that of DLC2. Taken together, we have provided the first evidence for mitochondrial localization of DLC2 through the START domain. These findings might have implications in liver physiology and carcinogenesis.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: David Chi-Heng
authors.initials: DC
authors.lastname: Ng
authors.forename: Shing-Fai
authors.initials: SF
authors.lastname: Chan
authors.forename: Kin Hang
authors.initials: KH
authors.lastname: Kok
authors.forename: Judy Wai Ping
authors.initials: JW
authors.lastname: Yam
authors.forename: Yick-Pang
authors.initials: YP
authors.lastname: Ching
authors.forename: Irene Oi-Lin
authors.initials: IO
authors.lastname: Ng
authors.forename: Dong-Yan
authors.initials: DY
authors.lastname: Jin
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D020690:GTPase-Activating Proteins; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000235:genetics; D021381:Protein Transport / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D020690:GTPase-Activating Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; C473607:STARD13 protein, human; D025521:Tumor Suppressor Proteins; D000098682:Steroidogenic Acute Regulatory Protein