title: Production of (R)-3-Chloro-1,2-Propanediol from Prochiral 1,3-Dichloro-2-Propanol by Corynebacterium sp. Strain N-1074.
abstract: The production of (R)-3-chloro-1,2-propanediol [(R)-MCP] from prochiral 1,3-dichloro-2-propanol (DCP) was examined with a bacterial strain identified as a Corynebacterium strain. The addition of glycerol as a carbon source or some chlorinated alcohols to a medium was effective for the induction of activity catalyzing the transformation of DCP into MCP. The optimum pH for (R)-MCP production by the resting cell reaction was around 8.0. The optical purity of (R)-MCP formed was improved by keeping the level of DCP in the reaction mixture at a low concentration. (R)-MCP was obtained from 77.5 mM DCP with a 97.3% molar conversion yield and an 83.8% enantiomeric excess of its optical purity by periodic feeding of the substrate.
authors.affiliation: Central Research Laboratory, Nitto Chemical Industry Co., Ltd., Tsurumi-ku, Yokohama 230, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: F
authors.initials: F
authors.lastname: Yu
authors.forename: W
authors.initials: W
authors.lastname: Mizunashi
authors.forename: I
authors.initials: I
authors.lastname: Watanabe