Correlation between lethal toxin-neutralizing antibody titers and protection from intranasal challenge with Bacillus anthracis Ames strain spores in mice after transcutaneous immunization with recombinant anthrax protective antigen.
Transcutaneous immunization of mice with recombinant protective antigen (rPA) of Bacillus anthracis resulted in significantly higher lethal toxin-neutralizing antibody titers than did intramuscular injection of alum-adsorbed rPA. Immunized mice were partially protected against intranasal challenge with 235,000 (10 50% lethal doses) Ames strain B. anthracis spores. A highly significant correlation was observed between toxin-neutralizing antibody titer and survival after challenge. Future experiments with rabbits and nonhuman primates should confirm the significance of protection by this vaccine strategy.
Peachman
Kristina K
KK
Department of Vaccine Production and Delivery, Division of Retrovirology, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, 1600 East Gude Drive, Rockville, MD 20850, USA.
Rao
Mangala
M
Alving
Carl R
CR
Burge
Robert
R
Leppla
Stephen H
SH
Rao
Venigalla B
VB
Matyas
Gary R
GR
D000279:Administration, Cutaneous; D000818:Animals; D000881:Anthrax / Q000276:immunology* / Q000517:prevention & control; D022122:Anthrax Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D001408:Bacillus anthracis / Q000235:genetics / Q000276:immunology*; D005260:Female; D051379:Mice; D008808:Mice, Inbred CBA; D013171:Spores, Bacterial / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D014614:Vaccines, Synthetic