[Study on population diversity and antimicrobial activity of actinomycete from acidic soil in Xizang area].
156 actinomycetes were isolated from 10 soil samples collected in Tibet by the method of DDC(dispersion and differential centrifugation). The isolates were assigned to 32 different groups on the basis of their morphological characters and colors of substrate mycelium, aerial hyphae and pigment. The result of cell-wall DAP on 65 representative strains selected from these groups was that 9 strains were different from Streptomyces. The analysis of ARDRA and 16S rDNA sequeces showed that the isolates belonged to the genus Streptomyces and five different rare actinomycete genera respectively. Antimicrobial activity of the representative strains was also tested, with the result that 38.5% isolates were positive.
Luo
Hong-li
HL
Institute of Modern Biopharmaceuticals, School of Life Science, South-West Normal University, Chongqing, China.
Huang
Ying
Y
Wang
Li-ming
LM
Liu
Zhi-heng
ZH
Xie
Jian-ping
JP
Hu
Chang-hua
CH
D039903:Actinobacteria / Q000302:isolation & purification* / Q000502:physiology; D000596:Amino Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012988:Soil Microbiology
D000596:Amino Acids; D012336:RNA, Ribosomal, 16S