title: Quantum dot-based multiplexed fluorescence resonance energy transfer.
abstract: We demonstrate the use of luminescent quantum dots (QDs) conjugated to dye-labeled protein acceptors for nonradiative energy transfer in a multiplexed format. Two configurations were explored: (1) a single color QD interacting with multiple distinct acceptors and (2) multiple donor populations interacting with one type of acceptor. In both cases, we showed that simultaneous energy transfer between donors and proximal acceptors can be measured. However, data analysis was simpler for the configuration where multiple QD donors are used in conjunction with one acceptor. Steady-state fluorescence results were corroborated by time-resolved measurements where selective shortening of QD lifetime was measured only for populations that were selectively engaged in nonradiative energy transfer.
authors.affiliation: U.S. Naval Research Laboratory, Optical Sciences Division, Code 5611, Washington, DC 20375, USA.
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Clapp
authors.forename: Igor L
authors.initials: IL
authors.lastname: Medintz
authors.forename: H Tetsuo
authors.initials: HT
authors.lastname: Uyeda
authors.forename: Brent R
authors.initials: BR
authors.lastname: Fisher
authors.forename: Ellen R
authors.initials: ER
authors.lastname: Goldman
authors.forename: Moungi G
authors.initials: MG
authors.lastname: Bawendi
authors.forename: Hedi
authors.initials: H
authors.lastname: Mattoussi
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D058619:Maltose-Binding Proteins; D045663:Quantum Dots
chemical_list: D002352:Carrier Proteins; D005456:Fluorescent Dyes; D058619:Maltose-Binding Proteins