title: Effect of supplementing terpenoid biosynthetic precursors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures.
abstract: The effect of precursor feeding on the production of bilobalide and ginkgolides was studied with suspension cell cultures of Ginkgo biloba. The precursors greatly influenced the productivity of bilobalide and ginkgolides. Precursor supplementation increased the accumulation of both bilobalide and ginkgolides, and with positive effect on cell growth. The GA accumulation by cell cultures was influenced by precursors upstream in the metabolism, whereas the BB accumulation was under the influence of downstream precursors of the terpenoid biosynthetic pathway. Furthermore, precursor feeding modified the ratios of the BB, GA and GB in cells and cell cultures of G. biloba. The studies also aid in understanding effect of precursor feeding on the bilobalide and ginkgolides biosynthetic pathway.
authors.affiliation: Division of Forest Science, Gyeongsang National University, Jinju 660-701, South Korea.
authors.forename: Seung-Mi
authors.initials: SM
authors.lastname: Kang
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Min
authors.forename: Yong-Duck
authors.initials: YD
authors.lastname: Kim
authors.forename: Dong-Jin
authors.initials: DJ
authors.lastname: Park
authors.forename: Ha-Na
authors.initials: HN
authors.lastname: Jung
authors.forename: Chandrakant S
authors.initials: CS
authors.lastname: Karigar
authors.forename: Yeong-Lae
authors.initials: YL
authors.lastname: Ha
authors.forename: Seon-Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Myung-Suk
authors.initials: MS
authors.lastname: Choi
mesh_terms: D046935:Bilobalides / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism* / Q000494:pharmacology; D020441:Ginkgo biloba / Q000187:drug effects* / Q000378:metabolism*; D046934:Ginkgolides / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D046935:Bilobalides; D017077:Culture Media, Conditioned; D046934:Ginkgolides; D013729:Terpenes