title: Dialysis Fermentor Systems for Concentrated Culture of Microorganisms.
abstract: Cell-mass production of Serratia marcescens was studied in dialysis systems in which growth was managed in a fermentor remote from, but connected by conduits and pumps with, a nutrient reservoir. Dialysis was accomplished with membrane tubing in either the fermentor or the reservoir, or best with membrane sheet in a plate-and-frame dialyzer that was remote from but connected with both vessels. Growth trials with these systems demonstrated their ability to produce virtually unlimited population density in a liquid culture, viable counts in excess of 10 cells/ml and partial cell volume of 50% being attained. The system used for growth also may be used, after the growth cycle, to concentrate cells still further by osmotic dehydration with a hydrophilic colloid. The dialyzer-dialysis system that was evolved permits independent control of the component operations and is believed to be adaptable to any desired scale of size.
authors.affiliation: Department of Microbiology, The University of Michigan, Ann Arbor, Michigan.
authors.forename: D M
authors.initials: DM
authors.lastname: Gallup
authors.forename: P
authors.initials: P
authors.lastname: Gerhardt