title: Peptide and small-molecule microarrays.
abstract: Two methods that use chemoselective ligation chemistry to prepare peptide and small-molecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.
authors.affiliation: Division of Hematology & Oncology, Department of Internal Medicine, UC Davis Cancer Center, University of California, Davis, USA.
authors.forename: Jan
authors.initials: J
authors.lastname: Marik
authors.forename: Kit S
authors.initials: KS
authors.lastname: Lam
mesh_terms: D000818:Animals; D005898:Glass / Q000737:chemistry; D006038:Glyoxylates / Q000737:chemistry; D007659:Ketones / Q000737:chemistry; D015394:Molecular Structure; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding; D012685:Sepharose / Q000737:chemistry
chemical_list: D006038:Glyoxylates; D007659:Ketones; D010455:Peptides; D012685:Sepharose