Cathepsin E is a specific marker of dysplasia in APC mouse intestine.
Transcriptional profiling of APC(Min/+) mouse intestinal epithelial tissue has revealed that cathepsin E (catE) manifests high relative expression in adenomas and carcinomas relative to normal epithelium. Real-time RT-PCR data presented previously confirm the presence of catE transcript in APC(Min/+) adenomatous cells compared with samples derived from normal APC(Min/+) and wild-type tissue. At the protein level, strong, highly specific immunohistochemical staining for catE is displayed in dysplastic lesions of APC(Min/+) mice. Using Western immunoblot analyses, it was additionally established that the urine of tumor-bearing mice contains higher levels of the monomeric form of catE than their wild-type counterparts. These results authenticate the relationship between transcript abundance and protein levels in transformed tissue and suggest potential utility for catE as a marker for the inception and progression of intestinal cancers.
Busquets
Lizette
L
Walther Cancer Center, Notre Dame, IN 46556, USA.
Guillen
Hector
H
DeFord
Melanie E
ME
Suckow
Mark A
MA
Navari
Rudolph M
RM
Castellino
Francis J
FJ
Prorok
Mary
M
D000236:Adenoma / Q000235:genetics* / Q000473:pathology; D000818:Animals; D015153:Blotting, Western; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D020059:Cathepsin E / Q000032:analysis* / Q000096:biosynthesis* / Q000652:urine; D018450:Disease Progression; D017491:Genes, APC; D007150:Immunohistochemistry; D007410:Intestinal Diseases / Q000235:genetics; D007414:Intestinal Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020059:Cathepsin E