Membrane organization and dynamics of the G-protein-coupled serotonin1A receptor monitored using fluorescence-based approaches.
The G-protein-coupled receptor (GPCR) superfamily represents one of the largest classes of molecules involved in signal transduction across the plasma membrane. Fluorescence-based approaches have provided valuable insights into GPCR functions such as receptor-receptor and receptor-ligand interactions, real-time assessment of signal transduction, receptor dynamics on the plasma membrane, and intracellular trafficking of receptors. This has largely been possible with the use of fluorescent probes such as the green fluorescent protein (GFP) from the jellyfish Aequoria victoria and its variants. We discuss the potential of fluorescence-based approaches in providing novel information on the membrane organization and dynamics of the G-protein-coupled serotonin1A receptor tagged to the enhanced yellow fluorescent protein (EYFP).
Pucadyil
Thomas J
TJ
Centre for Cellular and Molecular Biology, Hyderabad, India.
Kalipatnapu
Shanti
S
Chattopadhyay
Amitabha
A
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D002462:Cell Membrane / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics; D008164:Luminescent Proteins / Q000032:analysis* / Q000235:genetics; D044282:Receptor, Serotonin, 5-HT1A / Q000032:analysis* / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000032:analysis* / Q000235:genetics
D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D043562:Receptors, G-Protein-Coupled; C054562:yellow fluorescent protein, Bacteria; D044282:Receptor, Serotonin, 5-HT1A; D049452:Green Fluorescent Proteins