title: Adsorption of biometals to monosodium titanate in biological environments.
abstract: Monosodium titanate (MST) is an inorganic sorbent/ion exchanger developed for the removal of radionuclides from nuclear wastes. We investigated the ability of MST to bind Cd(II), Hg(II), Au(III), or the Au-organic compound auranofin to establish the utility of MST for applications in environmental decontamination or medical therapy (drug delivery). Adsorption isotherms for MST were determined at pH 7-7.5 in water or phosphate-buffered saline. The extent of metal binding was determined spectroscopically by measuring the concentrations of the metals in solution before and after contact with the MST. Cytotoxic responses to MST were assessed using THP1 monocytes and succinate dehydrogenase activity. Monocytic activation by MST was assessed by TNFalpha secretion (ELISA) with or without lipopolysaccharide (LPS) activation. MST adsorbed Cd(II), Hg(II), and Au(III) under conditions similar to those in physiological systems. MST exhibited the highest affinity for Cd(II) followed by Hg(II) and Au (III). MST (up to 100 mg/L) exhibited only minor (<25% suppression of succinate dehydrogenase) cytotoxicity and did not trigger TNFalpha secretion nor modulate LPS-induced TNFalpha secretion from monocytes. MST exhibits high affinity for biometals with no significant biological liabilities in these introductory studies. MST deserves further scrutiny as a substance with the capacity to decontaminate biological environments or deliver metals or metal compounds for therapeutic applications.
authors.affiliation: Savannah River National Laboratory, Westinghouse Savannah River Company, Aiken, South Carolina 29808, USA.
authors.forename: D T
authors.initials: DT
authors.lastname: Hobbs
authors.forename: R L W
authors.initials: RL
authors.lastname: Messer
authors.forename: J B
authors.initials: JB
authors.lastname: Lewis
authors.forename: D R
authors.initials: DR
authors.lastname: Click
authors.forename: P E
authors.initials: PE
authors.lastname: Lockwood
authors.forename: J C
authors.initials: JC
authors.lastname: Wataha
mesh_terms: D000327:Adsorption; D001310:Auranofin / Q000737:chemistry* / Q000627:therapeutic use; D001672:Biocompatible Materials; D002460:Cell Line; D003666:Decontamination; D016503:Drug Delivery Systems; D006801:Humans; D007474:Ion Exchange; D008070:Lipopolysaccharides / Q000494:pharmacology / Q000633:toxicity; D008422:Materials Testing; D019216:Metals, Heavy / Q000737:chemistry* / Q000627:therapeutic use; D009000:Monocytes / Q000378:metabolism / Q000648:ultrastructure; D011850:Radioactive Waste; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D008070:Lipopolysaccharides; D019216:Metals, Heavy; D011850:Radioactive Waste; D014409:Tumor Necrosis Factor-alpha; D001310:Auranofin; D013385:Succinate Dehydrogenase