The pseudorabies virus VP1/2 tegument protein is required for intracellular capsid transport.
Transport of capsids in cells is critical to alphaherpesvirus infection and pathogenesis; however, viral factors required for transport have yet to be identified. Here we provide a detailed examination of capsid dynamics during the egress phase of infection in Vero cells infected with pseudorabies virus. We demonstrate that the VP1/2 tegument protein is required for processive microtubule-based transport of capsids in the cytoplasm. A second tegument protein that binds to VP1/2, UL37, was necessary for wild-type transport but was not essential for this process. Both proteins were also required for efficient nuclear egress of capsids to the cytoplasm.
Luxton
G W Gant
GW
Department of Microbiology-Immunology, Ward Bldg., Rm. 10-105, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
Lee
Joy I-Hsuan
JI
Haverlock-Moyns
Sarah
S
Schober
Joseph Martin
JM
Smith
Gregory Allan
GA
D000818:Animals; D036022:Capsid Proteins / Q000378:metabolism*; D002522:Chlorocebus aethiops; D011558:Herpesvirus 1, Suid / Q000502:physiology*; D021381:Protein Transport; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000502:physiology*; D015678:Viral Structural Proteins / Q000502:physiology*; D014771:Virion / Q000502:physiology; D019065:Virus Assembly / Q000502:physiology*
D036022:Capsid Proteins; C084763:UL37 protein, Human herpesvirus 1; D014760:Viral Fusion Proteins; D015678:Viral Structural Proteins