title: Construction and characterization of a series of vectors for Schizosaccharomyces pombe.
abstract: A set of vectors was created to allow cloning and expression studies in Schizosaccharomyces pombe. These vectors had a uniform backbone with an efficient Sz. pombe ARS, ARS3002, but different selectable markers--his3+, leu1+, ade6+ and ura4+. The vectors functioned efficiently as autonomously replicating plasmids that could also be converted into integrating vectors. The ura4+-containing vector was used to construct a Sz. pombe genomic library.
authors.affiliation: Unit on Chromatin and Transcription, NICHD/NIH, Building 18T, Room 106, 18 Library Drive, Bethesda, MD 20892, USA.
authors.forename: C
authors.initials: C
authors.lastname: Adams
authors.forename: D
authors.initials: D
authors.lastname: Haldar
authors.forename: R T
authors.initials: RT
authors.lastname: Kamakaka
mesh_terms: D015723:Gene Library; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000138:chemical synthesis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*
chemical_list: D005819:Genetic Markers