[Enzyme immunoassay of ampicillin in milk].
An indirect immunoassay for quantitative determination of ampicillin (range, 10-1000 ng/ml) in buffer or milk has been developed. Polyclonal antibodies were obtained against ampicillin conjugated with bovine serum albumin; the conjugate was synthesized by direct condensation using carbodiimide. The antibodies were specific for ampicillin and exhibited low cross-reactivity to other penicillins (azlocillin, 17%; penicillin G, 10%; piperacillin, 5%; and carbenicillin, 4%). Matrix effects were minimized by combining the use of a casein-supplemented buffer (content of casein, 1%) with sample dilution. The threshold of ampicillin detection in milk (diluted tenfold) was equal to 5.0 ng/ml (which corresponded to 50 ng/ml of the original sample).
Samsonova
Zh V
ZhV
Shchelokova
O S
OS
Ivanova
N L
NL
Rubtsova
M Iu
MIu
Egorov
A M
AM
D000667:Ampicillin / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002021:Buffers; D002417:Cattle; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry; D007124:Immunoenzyme Techniques; D008892:Milk / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000900:Anti-Bacterial Agents; D000906:Antibodies; D002021:Buffers; D012710:Serum Albumin, Bovine; D000667:Ampicillin