Cytoprotective effect of green tea extract and quercetin against hydrogen peroxide-induced oxidative stress.
In this study, we evaluated the cytoprotective effects of antioxidative substances in hydrogen peroxide (H2O2) treated Mel-Ab melanocytes. Tested substances include selenium, quercetin, green tea (GT) extract, and several vitamins (ascorbic acid, Trolox, and folic acid). Of these, both quercetin and GT extract were found to have strong cytoprotective effects on H2O2-induced cell death. We also examined additive effects, but no combination of two of any of the above substances was found to act synergistically against oxidative damage in Mel-Ab cells. Nevertheless, a multi-combination of GT extract, quercetin, and folic acid appeared to prevent cellular damage in a synergistic manner, which suggests that combinations of antioxidants may be of importance, and that co-treatment with antioxidants offers a possible means of treating vitiligo, which is known to be related to melanocyte oxidative stress.
Jeong
Yun-Mi
YM
Department of Dermatology, Seoul National University College of Medicine, Korea.
Choi
Yeong-Gon
YG
Kim
Dong-Seok
DS
Park
Seo-Hyoung
SH
Yoon
Jin-A
JA
Kwon
Sun-Bang
SB
Park
Eun-Sang
ES
Park
Kyoung-Chan
KC
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D028244:Camellia / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020111:Chlorodiphenyl (54% Chlorine) / Q000494:pharmacology; D052244:Endocrine Disruptors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D008544:Melanocytes / Q000187:drug effects; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology*; D014815:Vitamins / Q000737:chemistry / Q000494:pharmacology
D000975:Antioxidants; D052244:Endocrine Disruptors; D010936:Plant Extracts; D014815:Vitamins; D020111:Chlorodiphenyl (54% Chlorine); D011794:Quercetin; D006861:Hydrogen Peroxide