[Determination of phenylarsenic compounds in environmental samples by high performance liquid chromatography].
A reversed-phase high performance liquid chromatographic method was developed for simultaneous determination of phenylarsonic acid (PAA), phenylarsine oxide (PAO), diphenylarsinic acid (DPAA), bis (diphenylarsine) oxide (BDPAO) and triphenylarsine (TPA) in soil samples. The extraction solvent for soil samples was acetonitrile and ultrasound was used in the extraction. Good separation was achieved by using an Inertsil CN-3 column (250 mm x 4.6 mm i.d., 5 microm) with the following conditions: gradient program of mobile phase, a 10 min linear gradient from 100% A (a mixture of acetonitrile-2.5 g/L KH2PO4 (pH 2.0) buffer (5:95, v/v) ) to 100% B ( a mixture of acetonitrile-2. 5 g/L KH2PO4 (pH 2.0) buffer (50:50, v/v)), and isocratic at 100% B from 10.01 min to 28 min; flow rate of 1.0 mL/min; time-programmed UV detection at 210 nm (0 - 10.5 min, 20 - 28 min) and 268 nm (10. 5 - 20 min). The linear range of PAA, PAO, DPAA, BDPAO and TPA were 8 - 30, 5 - 40, 20 -4000, 120 - 8000 and 1 - 60 mg/L, respectively. The detection limits at a signal-to-noise ratio of 3 were 0.1, 0.1, 0.2, 10, and 0.1 mg/L for PAA, PAO, DPAA, BDPAO and TPA, respectively.
Li
Shanmao
S
Analytical Center, Institute of Chemical Defence, Beijing 102205, China.
Li
Wei
W
Yue
Lijun
L
Zuo
Boli
B
D001152:Arsenicals / Q000032:analysis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results
D001152:Arsenicals; C029341:oxophenylarsine; C020979:benzenearsonic acid; C493087:diphenylarsinic acid