title: Strain-dependent expression of signaling proteins in the mouse hippocampus.
abstract: Individual mouse strains may differ significantly in terms of behavior and cognitive function. Hippocampal gene expression profiling on several mouse strains has been carried out and points toward substantial strain-specific variation of more than 200 genes including components of major signaling pathways involved in neuronal information storage. Strain-specific hippocampal protein expression, however, has not been investigated yet. A proteomic approach based on two-dimensional gel electrophoresis coupled with mass spectrometry has been chosen to address this question by determining strain-dependent expression of signaling proteins in hippocampi of four inbred and one outbred mouse strain. Forty-six spots corresponding to 37 different signaling proteins have been analyzed and quantified. Statistical analysis revealed strain-dependent expression of serine/threonine protein phosphatase 1, serine/threonine protein phosphatase 2A, large GTP binding protein OPA1, guanine nucleotide-binding protein beta, putative GTP-binding protein Ran, receptor of activated protein kinase C1, WASP-family protein member 1, voltage-dependent anion channel 2 and 14-3-3 protein gamma. Differential expression of signaling proteins in the hippocampus may contribute to the molecular understanding of strain-dependent behavioral and cognitive performance. Moreover, these data highlight the importance of the genetic background for the analysis of signaling pathways in the hippocampus in wild-type mice as well as in gene-targeting experiments.
authors.affiliation: Department of Pediatrics, Division of Pediatric Neuroscience, Medical University of Vienna, Währinger Gürtel 18, A-1090 Vienna, Austria.
authors.forename: D D
authors.initials: DD
authors.lastname: Pollak
authors.forename: J
authors.initials: J
authors.lastname: John
authors.forename: A
authors.initials: A
authors.lastname: Schneider
authors.forename: H
authors.initials: H
authors.lastname: Hoeger
authors.forename: Gert
authors.initials: G
authors.lastname: Lubec
mesh_terms: D000818:Animals; D004452:Echocardiography; D019204:GTP-Binding Proteins / Q000235:genetics / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism*; D007473:Ion Channels / Q000235:genetics / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009419:Nerve Tissue Proteins / Q000502:physiology*; D010641:Phenotype; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007473:Ion Channels; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D019204:GTP-Binding Proteins