title: Analysis of Poly-beta-Hydroxybutyrate in Rhizobium japonicum Bacteroids by Ion-Exclusion High-Pressure Liquid Chromatography and UV Detection.
abstract: Ion-exclusion high-pressure liquid chromatography (HPLC) was used to measure poly-beta-hydroxybutyrate (PHB) in Rhizobium japonicum bacteroids. The products in the acid digest of PHB-containing material were fractionated by HPLC on Aminex HPX-87H ion-exclusion resin for organic acid analysis. Crotonic acid formed from PHB during acid digestion was detected by its intense absorbance at 210 nm. The Aminex-HPLC method provides a rapid and simple chromatographic technique for routine analysis of organic acids. Results of PHB analysis by Aminex-HPLC were confirmed by gas chromatography and spectrophotometric analysis.
authors.affiliation: Department of Biochemistry and Interdisciplinary Plant Biochemistry-Physiology Group, University of Missouri-Columbia, Columbia, Missouri 65211.
authors.forename: D B
authors.initials: DB
authors.lastname: Karr
authors.forename: J K
authors.initials: JK
authors.lastname: Waters
authors.forename: D W
authors.initials: DW
authors.lastname: Emerich