Cardiomyocytes from human embryonic stem cells.
Terminal heart failure is characterized by a significant loss of cardiac myocytes. Stem cells represent a possibility for replacing these lost myocytes but the question of which stem cells are most ideally suited for cell transplantation therapies is still being addressed. Here, we consider human embryonic stem cells (HESC), derived from human embryos in this context. We review the methods used to induce their differentiation to cardiomyocytes in culture, their properties in relation to primary human cardiomyocytes and their ability to integrate into host myocardium. In addition, issues regarding their safety that need addressing before use in cell transplantation therapies, both generally and specifically in relation to the heart, are considered.
Passier
R
R
Hubrecht Laboratory and Interuniversity Cardiology Institute of the Netherlands, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Denning
C
C
Mummery
C
C
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D020869:Gene Expression Profiling; D006321:Heart / Q000196:embryology*; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*