[Effects of spermine and putrescine on the binding of the transcription factor NF-kappaB to specific DNA sequences].
Using fluorescence resonance energy transfer technique, effects of polyamines (spermine and putrescine) on the binding of p50 subunit of the nuclear transcription factor NF-kappaB to specific DNA sequences were studied in model experiments. It is demonstrated that polyamines in the concentration range from 1 to 500 mcM facilitate the binding of NF-kappB to specific DNA sequences. The data currently obtained agree with our previous predictions made by the computer modelling techniques. Our results presented in this work allow us to suggest that polyamines (excessive synthesis of which proceeds in tumor growth) may participate in the regulation of transcription of several oncogenes by the factor NF-kappaB.
Aleksandrova
N O
NO
Avilov
S V
SV
Zalietok
S P
SP
D004247:DNA / Q000737:chemistry*; D008956:Models, Chemical; D052002:NF-kappa B p50 Subunit / Q000737:chemistry*; D011700:Putrescine / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013096:Spermine / Q000737:chemistry*; D013997:Time Factors
D052002:NF-kappa B p50 Subunit; D011994:Recombinant Proteins; D013096:Spermine; D004247:DNA; D011700:Putrescine