title: A rapid HPLC-ESI-MS/MS for qualitative and quantitative analysis of saponins in "XUESETONG" injection.
abstract: 'XUESETONG' injection, one of the most widely used proprietary medicines in traditional Chinese medicine, consists of total saponins made from Panax notoginseng, which is a highly valued and important Chinese medicinal herb. It is used to treat cardiovascular diseases. In order to control the quality of XUESETONG injection, a rapid HPLC-ESI-MS/MS method was developed for qualitative and quantitative determination of the saponins. The analyses were performed on SB-C18 column using gradient elution in 25 min. Full scan and time programmed selected reaction monitoring (SRM) were used for qualitative and quantitative analysis of saponins, respectively. Twenty-seven saponins were identified and nine of them including notoginsenoside R1, ginsenoside Rb1, Rb2, Rb3, Rc, Rd, Re, Rf and Rg1 were quantified. Ten XUESETONG injections were analyzed and compared. The results showed that there is a great variation among different samples. In conclusion, the developed method is rapid, accurate and sensitive for qualitative and quantitative analysis of saponins in XUESETONG injection. Moreover, it also can be used for the quality control of P. notoginseng raw material and its preparations.
authors.affiliation: Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, PR China.
authors.forename: C M
authors.initials: CM
authors.lastname: Lai
authors.forename: S P
authors.initials: SP
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Yu
authors.forename: J B
authors.initials: JB
authors.lastname: Wan
authors.forename: K W
authors.initials: KW
authors.lastname: Kan
authors.forename: Y T
authors.initials: YT
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides / Q000032:analysis; D007267:Injections; D019999:Pharmaceutical Solutions / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D019999:Pharmaceutical Solutions; D012503:Saponins; C509799:xuesetong