Simultaneous measurement of urinary polyols using gas chromatography/mass spectrometry.
In the present study, we simultaneously measured several polyols, such as adonitol, arabitol, dulcitol, glucose, myo-inositol, mannitol, sorbitol, and xylitol, in urine by gas chromatography/mass spectrometry-positive chemical ionization. We also examined possible relationship between the levels of these metabolites and age in normal individuals. In order to proceed to its quantification by GC/MS, 200 microL of a urine sample were diluted with 3 ml of distilled water, lyophilized, acetylated, and then analyzed them. Using this method, we were able to quantify as little as 0.5-1.0 ng/microL, and we made the calibration curves to be linear from 0.25 to 250 ng/microL (r(2)>0.991). Analytical recoveries were over 89.4%, and the inter-day and intra-day variability for accuracy and reproducibility was less than 20%. In the normal urine sample, the levels of polyols were gender-differentiated and age-related. This simple GC/MS method is sensitive and allows the measurement of wide ranges of polyols using small amounts of urine. We conclude that the quantitation of urinary polyols using GC/MS appears to be a clinically useful method for assessing polyol-pathway activity.
Lee
Jeongae
J
Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Haweolgok-Dong 39-1, Sungbuk-Ku, Seoul 136-791, Republic of Korea.
Chung
Bong Chul
BC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000032:analysis; D006029:Glycosuria / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged; D013402:Sugar Alcohols / Q000652:urine*
D013402:Sugar Alcohols; D005947:Glucose