title: Cloning and Expression of a Schwanniomyces occidentalis alpha-Amylase Gene in Saccharomyces cerevisiae.
abstract: An alpha-amylase gene (AMY) was cloned from Schwanniomyces occidentalis CCRC 21164 into Saccharomyces cerevisiae AH22 by inserting Sau3AI-generated DNA fragments into the BamHI site of YEp16. The 5-kilobase insert was shown to direct the synthesis of alpha-amylase. After subclones containing various lengths of restricted fragments were screened, a 3.4-kilobase fragment of the donor strain DNA was found to be sufficient for alpha-amylase synthesis. The concentration of alpha-amylase in culture broth produced by the S. cerevisiae transformants was about 1.5 times higher than that of the gene donor strain. The secreted alpha-amylase was shown to be indistinguishable from that of Schwanniomyces occidentalis on the basis of molecular weight and enzyme properties.
authors.affiliation: Culture Collection and Research Center, Food Industry Research and Development Institute, P.O. Box 246, Hsinchu, Taiwan, Republic of China.
authors.forename: T T
authors.initials: TT
authors.lastname: Wang
authors.forename: L L
authors.initials: LL
authors.lastname: Lin
authors.forename: W H
authors.initials: WH
authors.lastname: Hsu