title: Bacterial aggregation in infected root canal.
abstract: The aim of this study was to investigate different microbial morphotypes in the root canal infection associated with chronic diffuse periapical lesion. In forty cases of asymptomatic teeth with radiographically diagnosed diffuse periapical lesion we took specimens of infected tissue from the root canals at the beginning of endodontic treatment. Fixation and four different staining methods of the specimens were obtained to provide microscope examination. All examined root canal specimens were heavily infected by bacteria. The most commonly identified were cocci 92 %, small mostly G+ diplococci and large G+cocci in clusters and grapelike groups, bacilli found in 67%, coccobacilli 37%, fungi 17%, and spirochetes in 5%.
authors.affiliation: Department of Dental Pathology and Endodontics, Faculty of Stomatology, University of Sarajevo, Bosnia and Herzegovina.
authors.forename: Amela
authors.initials: A
authors.lastname: Lacević
authors.forename: Nurija
authors.initials: N
authors.lastname: Bilalović
authors.forename: Aida
authors.initials: A
authors.lastname: Kapić
mesh_terms: D001419:Bacteria / Q000145:classification / Q000648:ultrastructure*; D002908:Chronic Disease; D004396:Coloring Agents; D003782:Dental Pulp / Q000382:microbiology; D003786:Dental Pulp Cavity / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000648:ultrastructure; D006094:Gram-Positive Bacteria / Q000648:ultrastructure; D006801:Humans; D010485:Periapical Periodontitis / Q000382:microbiology*
chemical_list: D004396:Coloring Agents