Mitochondrial DNA diagnosis for taeniasis and cysticercosis.
Molecular diagnosis for taeniasis and cysticercosis in humans on the basis of mitochondrial DNA analysis was reviewed. Development and application of three different methods, including restriction fragment length polymorphism analysis, base excision sequence scanning thymine-base analysis and multiplex PCR, were described. Moreover, molecular diagnosis of cysticerci found in specimens submitted for histopathology and the molecular detection of taeniasis using copro-DNA were discussed.
Yamasaki
Hiroshi
H
Department of Parasitology, Asahikawa Medical College, Japan. hyamasak@asahikawa-med.ac.jp
Nakao
Minoru
M
Sako
Yasuhito
Y
Nakaya
Kazuhiro
K
Sato
Marcello Otake
MO
Ito
Akira
A
D000818:Animals; D001483:Base Sequence; D051545:Cyclooxygenase 1 / Q000737:chemistry / Q000235:genetics; D003551:Cysticercosis / Q000175:diagnosis* / Q000469:parasitology; D018509:DNA, Helminth / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis*; D014644:Genetic Variation; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013621:Taenia / Q000145:classification / Q000235:genetics / Q000254:growth & development; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D051545:Cyclooxygenase 1