[Effect of magnetic mitomycin C nanoparticles on proliferation and apoptosis of L-02 cells in vitro].
OBJECTIVE
To investigate the effects of mitomycin magnetic polybutylcyanoacrylate nanoparticles (MMC-MPBCA- NP), magnetic polybutylcyanoacrylate nanoparticles (MPBCA-NP) and magnetic fluids (MF) and MMC on the proliferation and apoptosis of normal human liver cell strain (L-02 cells) in vitro.


METHODS
The effect of MMC, MMC-MPBCA-NP, MPBCA-NP, and MF on the apoptosis and cell cycle of L-02 cells was studied by flow cytometry. The changes in the cell proliferation in response to treatments of the preparations and agents were examined by MTT colorimetric assay.


RESULTS
MMC, MMC-MPBCA-NP, MPBCA-NP produced inhibition of the cell proliferation to different degrees but MF failed to result in cell proliferation inhibition. The results of MTT assay showed that MM, MPBCA-NP and MMC-MPBCA-NP all possessed concentration-dependent cytotoxicity, which increased with the concentration increment in comparison with the blank control.


CONCLUSIONS
The descending order of the 4 agents in their effect of L-02 cell strain growth inhibition is MF >MPBCA-NP>MMC-MPBCA-NP>MMC. MMC and MMC-MPBCA-NP can inhibit the proliferation and cause cell cycle changes of L-02 cells, while MPBCA-NP inhibits the cell proliferation without inducing cell cycle alterations.
Ren
Fei
F
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. renfeisky@tom.com
Yang
Xi-xiao
XX
Chen
Jian-hai
JH
D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D016503:Drug Delivery Systems; D004659:Enbucrilate; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D008099:Liver / Q000166:cytology; D008280:Magnetics; D016685:Mitomycin / Q000494:pharmacology*; D053758:Nanoparticles
D016685:Mitomycin; D004659:Enbucrilate