title: Transformation of organoarsenical species by the microflora of freshwater crayfish.
abstract: A study of the transformation of arsenic species by the microflora of the freshwater crayfish Procambarus clarkii was carried out. The study of the degradation of AB (arsenobetaine) was performed in aerobic conditions in two culture media (tryptic soy broth and saline medium) at two temperatures (30 and 8 degrees C). The microflora transformed AB into TMAO (trimethylarsine oxide), DMA (dimethylarsinate), MA (methylarsonate), and an unidentified compound (U1). The quickest transformations were carried out by microflora from hepatopancreas incubated in saline medium at 30 degrees C. The individualized study of other arsenic species [AC (arsenocholine), TETRA (tetramethylarsonium ion), TMAO, DMA, and MA] was also performed in saline medium. The only transformation observed was of AC into AB. The bacteria possibly responsible for AB degradation were isolated, identified by phenotypic and genotypic methods, and individually assayed for AB transformation. Only isolates allocated to the species Pseudomonas putida were able to metabolize AB.
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Apdo 73, 46100, Burjassot, Valencia, Spain.
authors.forename: Vicenta
authors.initials: V
authors.lastname: Devesa
authors.forename: Andreas
authors.initials: A
authors.lastname: Loos
authors.forename: Maria Angeles
authors.initials: MA
authors.lastname: Súñer
authors.forename: Dinoraz
authors.initials: D
authors.lastname: Vélez
authors.forename: Amparo
authors.initials: A
authors.lastname: Feria
authors.forename: Antonio
authors.initials: A
authors.lastname: Martínez
authors.forename: Rosa
authors.initials: R
authors.lastname: Montoro
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Sanz
mesh_terms: D000818:Animals; D001152:Arsenicals / Q000378:metabolism*; D003400:Astacoidea / Q000382:microbiology*; D001419:Bacteria / Q000378:metabolism*; D003470:Culture Media; D005618:Fresh Water; D043143:Hepatopancreas / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000378:metabolism; D016958:Pseudomonas putida / Q000378:metabolism; D013030:Spain
chemical_list: D001152:Arsenicals; D003470:Culture Media