title: Effect of nickelous and other metal ions on the inhibition of rumen bacterial metabolism by 3-(3'-isocyanocyclopent-2-enylidene)propionic Acid and related isocyanides.
abstract: 3-(3'-Isocyanocyclopent-2-enylidene)propionic acid at a concentration of 2 to 5 mug ml inhibited cellulose digestion by a mixed culture of rumen microorganisms and in other experiments inhibited the degradation of timothy hay (Phleum pratense) in a digestibility test. At isocyanide concentrations of 12 mug ml the fermentation activity of rumen fluid, measured by its dehydrogenase activity, was inhibited but not abolished. All of these isocyanide effects were reversed by the incorporation of nickelous ion into the solutions of the systems under study. The activity of 1 mol of isocyanide is reversed by about 1 mol of Ni and, in the case of the cellulose digestion test, by about 1 mol of Co. Of some 15 other ions tested only Pd and possibly chromium reversed the effect of the isocyanide.
authors.affiliation: National Research Council of Canada, Atlantic Research Laboratory, Halifax, Nova Scotia B3H 3Z1, Experimental Farm, Research Branch, Agriculture Canada, Nappan, Nova Scotia B0L 1C0, and Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon, Saskatchewan S7N 0W0, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Brewer
authors.forename: F W
authors.initials: FW
authors.lastname: Calder
authors.forename: G A
authors.initials: GA
authors.lastname: Jones
authors.forename: D
authors.initials: D
authors.lastname: Tanguay
authors.forename: A
authors.initials: A
authors.lastname: Taylor