title: Degradation of the Ferric Chelate of EDTA by a Pure Culture of an Agrobacterium sp.
abstract: A pure culture of an Agrobacterium sp. (deposited as ATCC 55002) that mineralizes the ferric chelate of EDTA (ferric-EDTA) was isolated by selective enrichment from a treatment facility receiving industrial waste containing ferric-EDTA. The isolate grew on ferric-EDTA as the sole carbon source at concentrations exceeding 100 mM. As the degradation proceeded, carbon dioxide, ammonia, and an unidentified metabolite(s) were produced; the pH increased, and iron was precipitated from solution. The maximum rate of degradation observed with sodium ferric-EDTA as the substrate was 24 mM/day. At a substrate concentration of 35 mM, 90% of the substrate was degraded in 3 days and 70% of the associated chemical oxygen demand was removed from solution. Less than 15% of the carbon initially present was incorporated into the cell mass. Significant growth of this strain was not observed with uncomplexed EDTA as the sole carbon source at comparable concentrations; however, the ferric chelate of propylenediaminetetraacetic acid (ferric-PDTA) did support growth.
authors.affiliation: Genencor International, 1870 Winton Road, South, Rochester, New York 14618, and Department of Botany and Microbiology, Auburn University, Auburn, Alabama 36899.
authors.forename: J J
authors.initials: JJ
authors.lastname: Lauff
authors.forename: D B
authors.initials: DB
authors.lastname: Steele
authors.forename: L A
authors.initials: LA
authors.lastname: Coogan
authors.forename: J M
authors.initials: JM
authors.lastname: Breitfeller