title: Ion-exchange chromatography followed by ESI-MS for quantitative analysis of sugar monophosphates from glucose catabolism.
abstract: The aim of this work is to establish a quantitative method to determine the ratio of [U-(13)C] labeled to unlabeled hexose monophosphates isolated from yeast extracts. This is accomplished by anion exchange chromatography and mobile phase desalting followed by electrospray (ESI) mass spectrometry. We test the method with the analysis of a sample of biological origin. Previously developed analytical techniques are not adequate to accomplish mass spectrometric analysis of these and other small monosaccharide systems because of interference from salt clusters. By lowering the ionic strength of the mobile phase and using a simplified injection system to the mass spectrometer, we were able to obtain data on the relative abundance of the hexose monophosphates.
authors.affiliation: Department of Chemistry, Washington University, One Brookings Drive, Campus Box 1134, 63130, St. Louis, MO, USA.|the Department of Chemistry and Biochemistry, University of Missouri, 63121, St. Louis, MO.
authors.forename: James J
authors.initials: JJ
authors.lastname: Walters
authors.affiliation: Department of Chemistry, Washington University, One Brookings Drive, Campus Box 1134, 63130, St. Louis, MO, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Grayson
authors.affiliation: Department of Chemistry, Washington University, One Brookings Drive, Campus Box 1134, 63130, St. Louis, MO, USA. mgross@wustl.edu.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Gross
authors.affiliation: Department of Biology, Washington University, St. Louis, Missouri, USA.
authors.forename: Maureen
authors.initials: M
authors.lastname: Hughes
authors.affiliation: Department of Biology, Washington University, St. Louis, Missouri, USA.
authors.forename: Georgia
authors.initials: G
authors.lastname: Shearer
authors.affiliation: Department of Biology, Washington University, St. Louis, Missouri, USA.
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Kohl
authors.affiliation: Department of Chemistry and Biochemistry, University of Missouri, St. Louis, Missouri, USA.
authors.forename: James
authors.initials: J
authors.lastname: Bashkin
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D005947:Glucose / Q000378:metabolism*; D007202:Indicators and Reagents; D010710:Phosphates / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002241:Carbohydrates; D007202:Indicators and Reagents; D010710:Phosphates; D005947:Glucose