[Capability and stability of degrading rice straw of composite microbial system MC1].
The capability of degrading rice straw of lignocellulolytic composite microbial system MC1 was investigated under different methods of preservation and temperatures treatments of 80 degrees C to 95 degrees C, and stability of composite microbial system MC1 was studied through the method of Denaturing Gradient Gel Electrophoresis (DGGE). The results indicate that the rice straw of 2% dry weight of medium can be degraded completely at 50 degrees C within 10 days under static culture. After 9 days inoculating MC1, the dry weight of rice straw, cellulose, hemicellulose and lignin content was degraded by 81% , 99%, 74% and 51%, respectively. Capability of cellulose degrading and stability of composite microbial system MC1 is sustained under 4 years of continuing subculture, 4 years of dry preservation at room temperature, 4 years of preservation at - 20 degrees C, 1 year of liquid preservation at room temperature and at 4 degrees C, and treatment of 90 degrees C for 30 min, respectively. Plate culture results show that composite microbial system MC1 are consisted of bacteria. The main DNA bands are not changed by the method of 16S rDNA PCR-DGGE after culture of six months so that microbial composition of MC1 is very stable.
Wang
Wei-Dong
WD
College of Agronomy and Biotechnology, China Agricultural University, Beiijng 100094, China.
Cui
Zong-Jun
ZJ
Wang
Xiao-Fen
XF
Niu
Jun-Ling
JL
Liu
Jian-Bin
JB
Igarashi
Yasuo
Y
D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002482:Cellulose / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D012275:Oryza; D018547:Plant Stems / Q000382:microbiology*
D002482:Cellulose; D008031:Lignin