title: On-line microdevice for stress proteomics.
abstract: The handling of the cells or tissues is essential for proteomics research or drug screening, where labor is not avoidable. The steps of cell wash, protein extraction, protein denaturing are complicated procedures in conventional method using centrifugation and pipetting in the laboratory. This is the bottle-neck for proteome research. To solve these problems, we propose to utilize the nanotechnology, which will improve the proteomics methodology. Utilizing the nanotechnology, we developed a novel microseparation system, where centrifugation and pipetting are needless. This system has a nanostructured microdevice, by which the cell handling, protein extraction, and antibody assay can be performed. Since cell transfer is needless, all cells are corrected without any loss during the cell-pretreatment procedures, which allowed high reproducibility and enabled the detection of low amount of protein expression. Utilizing the microdevice, we analyzed the stress induced proteins. We further succeeded the screening of food that was useful for immunity and found that an extraction from seaweed promoted the apoptosis of T-lymphoblastic cells. Here, we present an on-line microdevice for stress proteomics.
authors.affiliation: Department of Molecular and Pharmaceutical Biotechnology, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Tabuchi
authors.forename: Hideya
authors.initials: H
authors.lastname: Nagata
authors.forename: Masanori
authors.initials: M
authors.lastname: Nomura
authors.forename: Sawako
authors.initials: S
authors.lastname: Katsube
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sawada
authors.forename: Akira
authors.initials: A
authors.lastname: Joko
authors.forename: Koji
authors.initials: K
authors.lastname: Hatta
authors.forename: Naoto
authors.initials: N
authors.lastname: Hagiwara
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Fumiyo
authors.initials: F
authors.lastname: Tomita
authors.forename: Satoko
authors.initials: S
authors.lastname: Miki
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Arai
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishiguro
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D009862:Online Systems; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D020543:Proteome