title: Sulfate reduction relative to methane production in high-rate anaerobic digestion: technical aspects.
abstract: The effect of different substrates and different levels of sulfate and sulfide on methane production relative to sulfate reduction in high-rate anaerobic digestion was evaluated. Reactors could be acclimated so that sulfate up to a concentration of 5 g of sulfate S per liter did not significantly affect methanogenesis. Higher levels gave inhibition because of salt toxicity. Sulfate reduction was optimal at a relatively low level of sulfate, i.e., 0.5 g of sulfate S per liter, but was also not significantly affected by higher levels. Both acetoclastic and hydrogenotrophic methane-producing bacteria adapted to much higher levels of free H(2)S than the values reported in the literature (50% inhibition occurred only at free H(2)S levels of more than 1,000 mg/liter). High levels of free H(2)S affected the sulfate-reducing bacteria only slightly. Formate and acetate supported the sulfate-reducing bacteria very poorly. In the high-rate reactors studied, intensive H(2)S formation occurred only when H(2) gas or an H(2) precursor such as ethanol was supplied.
authors.affiliation: Laboratory of Microbial Ecology, State University of Ghent, Coupure L 653, B-9000 Ghent, Belgium.
authors.forename: Z
authors.initials: Z
authors.lastname: Isa
authors.forename: S
authors.initials: S
authors.lastname: Grusenmeyer
authors.forename: W
authors.initials: W
authors.lastname: Verstraete