Invasion of the intestinal tract by sporozoites of Eimeria coecicola and Eimeria intestinalis in naive and immune rabbits.
Naive and immune specific-pathogen-free rabbits were inoculated in the duodenum with sporocysts of Eimeria coecicola or Eimeria intestinalis. Samples were taken from the following tissues: duodenum (site of penetration of sporozoites), ileum (specific target site of the endogenous development of E. intestinalis), vermiform appendix (target site of E. coecicola) and two extraintestinal sites, mesenteric lymph nodes (MLNs), and spleen. The presence of sporozoites was checked by immunohistochemistry. In rabbits primary-infected with E. coecicola, large numbers of sporozoites were detected in the duodenum, extraintestinal sites, and vermiform appendix. The abundance of sporozoites in the spleen, MLN, and appendix was significantly reduced in the immune rabbits, and the migration seemed impeded. In the rabbits infected with E. intestinalis, sporozoites were absent in the spleen and MLN, indicating that the route of migration is different from that of E. coecicola. The number of sporozoites in the crypts of the ileum was markedly reduced in the immune animals.
Pakandl
Michal
M
Institute of Parasitology, Academy of Sciences of Czech Republic, Branisovská 31, 37005, Ceské Budejovice, Czech Republic. pakandl@paru.cas.cz
Sewald
Brigitte
B
Drouet-Viard
Françoise
F
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000378:metabolism; D003048:Coccidiosis / Q000276:immunology / Q000469:parasitology*; D004539:Eimeria / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D006790:Host-Parasite Interactions; D007411:Intestinal Diseases, Parasitic; D007421:Intestine, Small / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D008198:Lymph Nodes / Q000469:parasitology; D010581:Peyer's Patches / Q000378:metabolism / Q000469:parasitology; D011817:Rabbits; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000469:parasitology; D034101:Sporozoites / Q000254:growth & development / Q000276:immunology / Q000473:pathology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan