Synthesis of 1,2-Epoxyoctane by Pseudomonas oleovorans During Growth in a Two-Phase System Containing High Concentrations of 1-Octene.
We have optimized and compared the synthesis of 1,2-epoxyoctane from 1-octene by resting and by growing cells of Pseudomonas oleovorans. The net production of 1,2-epoxyoctane by resting cells never exceeded 0.6 mg/ml of suspension. In contrast, P. oleovorans produced much more epoxide when it was grown on high levels of 1-octene. To raise the total production of epoxide, the octene layer was repeatedly transferred to fresh, growing cultures of P. oleovorans. By using this approach, a maximum of 28 mg of epoxide was synthesized per ml of total culture, resulting in the accumulation of ca. 75 mg of epoxide per ml in the octene phase.
de Smet
M J
MJ
Biochemisch Laboratorium, The University, 9747 AG Groningen, The Netherlands.
Wynberg
H
H
Witholt
B
B