title: Short interspersed elements (SINEs) are a major source of canine genomic diversity.
abstract: SINEs are retrotransposons that have enjoyed remarkable reproductive success during the course of mammalian evolution, and have played a major role in shaping mammalian genomes. Previously, an analysis of survey-sequence data from an individual dog (a poodle) indicated that canine genomes harbor a high frequency of alleles that differ only by the absence or presence of a SINEC_Cf repeat. Comparison of this survey-sequence data with a draft genome sequence of a distinct dog (a boxer) has confirmed this prediction, and revealed the chromosomal coordinates for >10,000 loci that are bimorphic for SINEC_Cf insertions. Analysis of SINE insertion sites from the genomes of nine additional dogs indicates that 3%-5% are absent from either the poodle or boxer genome sequences--suggesting that an additional 10,000 bimorphic loci could be readily identified in the general dog population. We describe a methodology that can be used to identify these loci, and could be adapted to exploit these bimorphic loci for genotyping purposes. Approximately half of all annotated canine genes contain SINEC_Cf repeats, and these elements are occasionally transcribed. When transcribed in the antisense orientation, they provide splice acceptor sites that can result in incorporation of novel exons. The high frequency of bimorphic SINE insertions in the dog population is predicted to provide numerous examples of allele-specific transcription patterns that will be valuable for the study of differential gene expression among multiple dog breeds.
authors.affiliation: The Institute for Genomic Research, Rockville, Maryland 20850, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ewen F
authors.initials: EF
authors.lastname: Kirkness
mesh_terms: D000818:Animals; D001483:Base Sequence; D001947:Breeding; D033481:Chromosomes, Mammalian / Q000235:genetics; D004285:Dogs / Q000145:classification / Q000235:genetics*; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D016678:Genome; D008297:Male; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012689:Sequence Homology, Nucleic Acid; D020086:Short Interspersed Nucleotide Elements / Q000235:genetics*