Snf1p-dependent Spt-Ada-Gcn5-acetyltransferase (SAGA) recruitment and chromatin remodeling activities on the HXT2 and HXT4 promoters.
We previously showed that the Spt-Ada-Gcn5-acetyltransferase (SAGA) complex is recruited to the activated HXT2 and HXT4 genes and plays a role in the association of TBP-associated factors. Using the HXT2 and HXT4 genes, we now present evidence for a functional link between Snf1p-dependent activation, recruitment of the SAGA complex, histone H3 removal, and H3 acetylation. Recruitment of the SAGA complex is dependent on the release of Ssn6p-Tup1p repression by Snf1p. In addition, we found that the Gcn5p subunit of the SAGA complex preferentially acetylates histone H3K18 on the HXT2 and HXT4 promoters and that Gcn5p activity is required for removal of histone H3 from the HXT4 promoter TATA region. In contrast, histone H3 removal from the HXT2 promoter does not require Gcn5p. In conclusion, although similar protein complexes are involved, induction of HXT2 and HXT4 displays important mechanistic differences.
van Oevelen
Chris J C
CJ
Department of Physiological Chemistry, Division of Biomedical Genetics, University Medical Center Utrecht, The Netherlands.
van Teeffelen
Hetty A A M
HA
van Werven
Folkert J
FJ
Timmers
H Th Marc
HT
D000107:Acetylation; D042002:Chromatin Assembly and Disassembly; D004268:DNA-Binding Proteins / Q000502:physiology; D051246:Glucose Transport Proteins, Facilitative; D051548:Histone Acetyltransferases / Q000502:physiology; D006657:Histones / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D012097:Repressor Proteins / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D016385:TATA Box; D015534:Trans-Activators / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
D004268:DNA-Binding Proteins; D051246:Glucose Transport Proteins, Facilitative; D051548:Histone Acetyltransferases; D006657:Histones; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D017346:Protein Serine-Threonine Kinases; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; C084498:HXT2 protein, S cerevisiae; C090593:HXT4 protein, S cerevisiae; C085976:MIG1 protein, S cerevisiae; C507785:SAGA complex, S cerevisiae; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor; C071570:SNF1-related protein kinases