Cytoskeletal networks and pathways involved in endocytosis.
Until recently, the actin cytoskeleton and the endocytic machinery were thought to operate independently. However, the actin cytoskeleton is an integral part of the cell cortex and there is growing evidence in diverse eukaryotes that F-actin plays a direct role during endocytic internalization. Genetic studies in Saccharomyces cerevisiae have demonstrated that Arp2/3-mediated F-actin assembly is required specifically for the internalization step of endocytosis. Using real-time image analysis, we recently defined a pathway for receptor-mediated endocytosis in budding yeast. Many features of this pathway appear to be conserved widely, indicating that principles derived from our studies in yeast will be directly applicable in more complex eukaryotes. We are pursuing our yeast studies using a combined approach involving image analysis, functional genomics, proteomics and biochemistry. These ongoing studies are providing a broader and deeper understanding of the molecular events of endocytosis, of how forces for actin polymerization are harnessed, and of how steps in the pathway are regulated. Our studies in mammalian cells provide evidence that this pathway is conserved in more complex organisms for endocytic and Golgi trafficking events.
Drubin
David G
DG
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3202, USA.
Kaksonen
Marko
M
Toret
Christopher
C
Sun
Yidi
Y
D000199:Actins / Q000378:metabolism*; D003599:Cytoskeleton / Q000737:chemistry / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology*; D046912:Multiprotein Complexes; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D000199:Actins; D046912:Multiprotein Complexes