title: Expressed sequence tags analysis of Blattella germanica.
abstract: Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.
authors.affiliation: Department of Otorhinolaryngology, Kosin University College of Medicine, Busan, Korea.
authors.forename: Hyang Suk
authors.initials: HS
authors.lastname: Chung
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Yu
authors.forename: Bong Jin
authors.initials: BJ
authors.lastname: Kim
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Kim
authors.forename: Joo Yeong
authors.initials: JY
authors.lastname: Kim
authors.forename: Hak Sun
authors.initials: HS
authors.lastname: Yu
authors.forename: Hae Jin
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Mee Sun
authors.initials: MS
authors.lastname: Ock
mesh_terms: D000818:Animals; D001483:Base Sequence; D020048:Blattellidae / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment