title: Immobilization of cryopreserved primary rat hepatocytes for the development of a bioartificial liver system.
abstract: Primary rat hepatocytes were cryopreserved in hormonally-defined medium (HDM) containing 40% (v/v) fetal bovine serum (FBS) and 10% (v/v) dimethyl sulfoxide (DMSO) in liquid N2 for 6 months. After thawing, the cells were immobilized using 2% (w/v) alginate and 0.5% (w/v) chitosan solutions. The capacities of ammonia removal and urea synthesis of the immobilized-thawed hepatocytes were similar to those of immobilized hepatocytes without cryopreservation. This result shows that immobilized hepatocytes after cryopreservation are useful for the development of a bioartificial liver system.
authors.affiliation: Department of Biotechnology and Bioengineering, Pukyong National University, 608-737, Busan, Korea.
authors.forename: Jeong Hwa
authors.initials: JH
authors.lastname: Son
authors.forename: Yu-Mi
authors.initials: YM
authors.lastname: Ha
authors.forename: Yun-Im
authors.initials: YI
authors.lastname: Kim
authors.forename: Ki Myong
authors.initials: KM
authors.lastname: Kim
authors.forename: Jung-Keug
authors.initials: JK
authors.lastname: Park
authors.forename: Sung-Koo
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques; D018914:Cells, Immobilized; D048271:Chitosan / Q000737:chemistry; D015925:Cryopreservation; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D019164:Liver, Artificial; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012996:Solutions / Q000737:chemistry*; D013997:Time Factors
chemical_list: D000464:Alginates; D012996:Solutions; D048271:Chitosan