Analysis of costimulatory molecule expression on antigen-specific T and B cells during the induction of adjuvant-induced Th1 and Th2 type responses.
Previous studies show that the generation of maximal T cell responses requires B cell antigen presentation and the differential expression of costimulatory molecules by B cells may affect polarization of naïve T cells to Th1 or Th2 phenotypes. We have therefore characterized the expression of activation and costimulatory molecules on antigen-specific T and B cells following immunisation with Alum or Alum/LPS to induce Th2 or Th1 responses in vivo. While antigen-specific B cells show similar levels of activation with respect to MHCII upregulation following Th1 or Th2 induction, they differentially express costimulatory molecules. Although ICOS-B7RP-1 interactions were originally implicated in Th2 generation, surprisingly this receptor-ligand pair was only upregulated on antigen-specific T and B cells following Th1 induction. In conclusion, these studies indicate that during the generation of antigen-specific Th1 or Th2 responses, adjuvants induce differential costimulation in antigen-specific B cells that may subsequently influence T cell polarization.
Smith
Karen M
KM
Division of Immunology, Infection & Inflammation, University of Glasgow, Glasgow, G11 6NT, UK. kms12a@clinmed.gla.ac.uk
Garside
Paul
P
McNeil
Robbie C
RC
Brewer
James M
JM
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000534:Alum Compounds / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology*; D018122:B7-1 Antigen / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D060945:Inducible T-Cell Co-Stimulator Ligand; D060889:Inducible T-Cell Co-Stimulator Protein; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D053278:OX40 Ligand; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D048069:Tumor Necrosis Factors / Q000032:analysis; D015854:Up-Regulation
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D018122:B7-1 Antigen; D000949:Histocompatibility Antigens Class II; C556694:Icos protein, mouse; D060945:Inducible T-Cell Co-Stimulator Ligand; D060889:Inducible T-Cell Co-Stimulator Protein; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D053278:OX40 Ligand; C504586:Tnfsf4 protein, mouse; D048069:Tumor Necrosis Factors; C041524:aluminum sulfate; D010047:Ovalbumin