title: Association of the Phe206Leu allele of the L-selectin gene with coronary artery disease.
abstract: OBJECTIVE
  The aim of this study was to assess the association between the L-selectin Phe206Leu polymorphism and coronary artery disease.
  
  
  METHODS
  A total of 322 patients (221 men and 101 women) with coronary artery disease in one or more vessels documented by angiography were studied; 157 subjects (85 men and 72 women) without atherosclerosis were included as controls. All subjects were genotyped for the L-selectin Phe206Leu gene polymorphism using polymerase chain reaction with sequence-specific primer (PCR-SSP). To assess disease severity, all patients were classified by numbers of coronary arteries with 50% stenosis.
  
  
  RESULTS
  A significantly increased frequency of the 206Leu mutant allele was observed in patients with coronary artery disease compared to the controls. The 206Leu allele frequency occurred in 42% of the patients with coronary artery disease compared to 30% of the controls (p<0.009). No association was found between the severity of coronary artery disease and the L-selectin Phe206Leu polymorphism.
  
  
  CONCLUSIONS
  Our findings suggest that carriage of L-selectin 206Leu mutant allele could contribute to susceptibility of Iranian individuals to contracting coronary artery disease.
authors.affiliation: Department of Immunology, Molecular Medicine Research Center, Hamedan University of Medical Sciences, Hamedan, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hajilooi
authors.forename: Nader
authors.initials: N
authors.lastname: Tajik
authors.forename: Arashmidos
authors.initials: A
authors.lastname: Sanati
authors.forename: Hossein
authors.initials: H
authors.lastname: Eftekhari
authors.forename: Ahmad
authors.initials: A
authors.lastname: Massoud
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007492:Iran / Q000453:epidemiology; D019041:L-Selectin / Q000235:genetics*; D007930:Leucine; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D010649:Phenylalanine
chemical_list: D019041:L-Selectin; D010649:Phenylalanine; D007930:Leucine