Immunization with persistent attenuated Delta lpg2 Leishmania major parasites requires adjuvant to provide protective immunity in C57BL/6 mice.
Leishmania major parasites lacking the GDP-mannose transporter, termed Deltalpg2 parasites, fail to induce disease in mice but persist long-term. We previously found that Deltalpg2 organisms protect BALB/c mice from virulent L. major challenge. In contrast, we report here that Deltalpg2 parasites induce protective immunity in C57BL/6 mice only when administered with CpG-containing oligodeoxynucleotides, indicating that parasite persistence alone is not sufficient to maintain protective immunity to L. major.
Kébaïer
Chahnaz
C
Department of Pathobiology, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104, USA.
Uzonna
Jude E
JE
Beverley
Stephen M
SM
Scott
Phillip
P
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D006028:Glycosphingolipids / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D018320:Leishmania major / Q000235:genetics / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000517:prevention & control; D008565:Membrane Proteins / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D015800:Protozoan Proteins / Q000235:genetics* / Q000502:physiology
D000276:Adjuvants, Immunologic; D006028:Glycosphingolipids; C095909:LPG2 protein, Leishmania; D008565:Membrane Proteins; D015800:Protozoan Proteins