The conserved oligomeric Golgi complex acts in organ morphogenesis via glycosylation of an ADAM protease in C. elegans.
In C. elegans, the gonad acquires two U-shaped arms through directed migration of gonadal distal tip cells (DTCs). A member of the ADAM (a disintegrin and metalloprotease) family, MIG-17, is secreted from muscle cells and localizes to the gonadal basement membrane where it functions in DTC migration. Mutations in cogc-3 and cogc-1 cause misdirected DTC migration similar to that seen in mig-17 mutants. Here, we report that COGC-3 and COGC-1 proteins are homologous to mammalian COG-3/Sec34 and COG-1/ldlBp, respectively, two of the eight components of the conserved oligomeric Golgi (COG) complex required for Golgi function. Knockdown of any of the other six components by RNA interference also produces DTC migration defects, suggesting that the eight components function in a common pathway. COGC-3 and COGC-1 are required for the glycosylation and gonadal localization of MIG-17, but not for secretion of MIG-17 from muscle cells. Furthermore, COGC-3 requires MIG-17 activity for its action in DTC migration. Our findings demonstrate that COG complex-dependent glycosylation of an ADAM protease plays a crucial role in determining organ shape.
Kubota
Yukihiko
Y
RIKEN Center for Developmental Biology, Chuo-ku, Kobe 650-0047, Japan.
Sano
Mitsue
M
Goda
Saori
S
Suzuki
Norio
N
Nishiwaki
Kiyoji
K
D051722:ADAM Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018509:DNA, Helminth / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D006031:Glycosylation; D006056:Golgi Apparatus / Q000201:enzymology; D006066:Gonads / Q000166:cytology / Q000254:growth & development; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009154:Mutation; D034622:RNA Interference; D017386:Sequence Homology, Amino Acid
D029742:Caenorhabditis elegans Proteins; D018509:DNA, Helminth; D051722:ADAM Proteins