title: Genetic Transformation of an argB Mutant of Aspergillus oryzae.
abstract: An argB mutant of Aspergillus oryzae NRRL 492 has been genetically transformed with the Aspergillus nidulans argB gene. Protoplasts were generated with a combination of Novozyme 234 and beta-glucuronidase and regenerated on sucrose-stabilized minimal medium without arginine as described for A. nidulans. A frequency of 5 to 10 transformants per mug of DNA was obtained; however, most transformants appeared abortive. The A. nidulans argB gene and vector sequences appeared to be integrated into the A. oryzae chromosome.
authors.affiliation: Department of Food Science, Cornell University, Ithaca, New York 14853.
authors.forename: Y T
authors.initials: YT
authors.lastname: Hahm
authors.forename: C A
authors.initials: CA
authors.lastname: Batt