Characterization of Amylolytic Enzyme Activities Associated with the Hyperthermophilic Archaebacterium Pyrococcus furiosus.
The hyperthermophilic archaebacterium Pyrococcus furiosus produces several amylolytic enzymes in response to the presence of complex carbohydrates in the growth medium. These enzyme activities, alpha-glucosidase, pullulanase, and alpha-amylase, were detected in both cell extracts and culture supernatants. All activities were characterized by temperature optima of at least 100 degrees C as well as a high degree of thermostability. The existence of this collection of activities in P. furiosus suggests that polysaccharide availability in its growth environment is a significant aspect of the niche from which it was isolated.
Brown
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Department of Chemical Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, and Center of Marine Biotechnology, University of Maryland, Baltimore, Maryland 21202.
Costantino
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HR
Kelly
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RM