Transcriptional regulation of human CC chemokine CCL15 gene by NF-kappaB and AP-1 elements in PMA-stimulated U937 monocytoid cells.
CCL15 exerts biological effects on a variety of cells, including monocytes. NF-kappaB has been reported to be involved in the transcription of the CCL15 gene. In this study, we have identified an AP-1 element located at -76/-65, which appears to regulate the transcription of the CCL15 gene. We also confirmed that the AP-1 factor binds to the element. Specific inhibitors for MAPK pathways and expression of dominant negative MKK4 or JNK1 reduced PMA-induced transcriptional activation of CCL15. Our findings indicate that transcription of the CCL15 gene is regulated by AP-1 and NF-kappaB through MEK and JNK MAPK pathways in monocytoid cells.
Shin
Yong-Hyun
YH
Graduate School of Biotechnology and Institute of Life Sciences and Resources, Kyung Hee University, Yongin 449-701, Korea.
Son
Kyung-No
KN
Lee
Guy Wilhem
GW
Kwon
Byoung S
BS
Kim
Jiyoung
J
D019742:Chemokines, CC / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D019402:Macrophage Inflammatory Proteins; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D015846:Monokines / Q000235:genetics*; D016328:NF-kappa B / Q000378:metabolism*; D017354:Point Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011485:Protein Binding; D020218:Response Elements / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*; D020298:U937 Cells
C108814:CCL15 protein, human; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D013755:Tetradecanoylphorbol Acetate