title: Bioactive heparin immobilized onto microfluidic channels in poly(dimethylsiloxane) results in hydrophilic surface properties.
abstract: A new composition of heparin coating for microfluidic systems made out of poly(dimethylsiloxane) (PDMS) was developed and evaluated. The coating that consists of a conditioning polyamine layer followed by two heparin/glutaraldehyde layers, resulted in channel surfaces with sufficient wettability to obtain flow of human normal plasma by capillary force alone. Hydrophilic channel walls are a desirable characteristic in microfluidic devices, since alternative pumping mechanisms must otherwise be included into the system. The immobilized heparin showed high antithrombin-binding capacity and a low degree of blood-material interaction. Plasma in contact with heparin-coated PDMS formed no detectable fibrin in a spectrophotometric assay by which plasma in contact with non-treated PDMS showed complete coagulation. The quartz crystal microbalance technique with energy dissipation monitoring (QCM-D) was utilized to obtain detailed information regarding adsorption kinetics and structural properties of the different layers composing the heparin coating.
authors.affiliation: Department of Engineering Sciences, Angström Laboratory, Uppsala University, Sweden.
authors.forename: Sara
authors.initials: S
authors.lastname: Thorslund
authors.forename: Javier
authors.initials: J
authors.lastname: Sanchez
authors.forename: Rolf
authors.initials: R
authors.lastname: Larsson
authors.forename: Fredrik
authors.initials: F
authors.lastname: Nikolajeff
authors.forename: Jonas
authors.initials: J
authors.lastname: Bergquist
mesh_terms: D001783:Blood Flow Velocity; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics / Q000379:methods*; D012016:Reference Values; D012828:Silicones / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon; D006493:Heparin