Production of 5' Nucleotide by Using Halophilic Nuclease H Preferentially Adsorbed on Flocculated Cells of the Halophile Micrococcus varians subsp. halophilus.
A bioreactor with a column of flocculated cells of the moderate halophile Micrococcus varians subsp. halophilus which adsorbed the halophilic nuclease H was designed to be used in the production of 5' nucleotides from RNA. A remarkable characteristic of the flocculated cells was that they preferentially adsorbed much exogenous nuclease, excluding adsorbed 5' nucleotidase. Furthermore, desalting treatment of the flocculated cells in the presence of 2% MgSO(4) . 7H(2)O gave rise to selective inactivation of 5' nucleotidase without the loss of nuclease H activity, and 5'-guanylic acid was produced with the bioreactor.
Onishi
H
H
Department of Agricultural Chemistry, Kagoshima University, Korimoto, Kagoshima-shi, Japan 890; Noda Institute for Scientific Research, Noda-shi, Chiba-ken, Japan 278 ; and Hiratsuka Research Laboratory, Sumitomo Heavy Industries, Ltd., Yuhigaoka, Hiratsuka-shi, Kanagawa-ken, Japan 254.
Kamekura
M
M
Yokoi
H
H
Kobayashi
T
T