Expression of interleukin-8 receptors (CXCR1 and CXCR2) in premenopausal women with recurrent urinary tract infections.
The migration of neutrophils through infected tissues is mediated by the CXC chemokines and its receptors (CXCR1 and CXCR2). It has been proposed that a CXCR1 deficiency could confer susceptibility to acute pyelonephritis in children. The objective of the study is to assess the surface expression of CXCR1 and CXCR2 and the existence of polymorphisms in the CXCR1 gene in premenopausal women with recurrent urinary tract infections. The study included 20 premenopausal women with recurrent urinary infections, with normal urinary tracts, and without diseases potentially associated with relapsing urinary infections and 30 controls without previous urinary infections. The levels of CXCR1 and CXCR2 expression on neutrophils were measured and analyzed by flow cytometry by measuring the mean fluorescence intensity (MFI) channel. The promoter and coding regions of the CXCR1 gene were analyzed for the presence of polymorphisms by a sequence-based typing method. Patients with recurrent urinary tract infections exhibited median levels of CXCR1 expression, determined from MFI values, similar to those of the controls. The analysis of CXCR2 showed that patients with recurrent urinary infections had lower median levels of expression, determined from the MFI values, than the controls (P = 0.002, Mann-Whitney U test). No polymorphisms were detected at the promoter or at the exon 1 region of the CXCR1 gene either in the patients or in the controls. Polymorphisms were detected at the exon 2 of CXCR1, but their frequencies did not differ between patients and controls. We have found a low level of CXCR2 expression in patients with recurrent urinary tract infections. These results suggest that a low level of CXCR2 expression may increase the susceptibilities of premenopausal women to urinary tract infections.
Smithson
Alex
A
Infectious Diseases Unit, Hospital Clinic, IDIBAPS, Edificio Helios, 1S planta. C/ Villarroel 170, 08036 Barcelona, Spain. asa30412@hotmail.com
Sarrias
Maria Rosa
MR
Barcelo
Juanjo
J
Suarez
Belen
B
Horcajada
Juan Pablo
JP
Soto
Sara Maria
SM
Soriano
Alex
A
Vila
Jordi
J
Martinez
Jose Antonio
JA
Vives
Jordi
J
Mensa
Jose
J
Lozano
Francisco
F
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D009504:Neutrophils / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D023062:Receptors, Interleukin-8A / Q000032:analysis* / Q000235:genetics; D023063:Receptors, Interleukin-8B / Q000032:analysis*; D012008:Recurrence; D014552:Urinary Tract Infections / Q000097:blood*
D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B