Quantitative analysis of PPARdelta mRNA by real-time RT-PCR in 86 rectal cancer tissues.
OBJECTIVE
The purpose of this study is to clarify the expression change of PPARdelta gene in human colorectal cancer tissues.


METHODS
Applying real-time RT-PCR, we quantified PPARdelta mRNA in a series of 86 tissues from excised primary rectal cancers. In each case, accompanying normal mucosa was collected for comparison.


RESULTS
Among the 86 rectal cancer tissues, 48 cases showed PPARdelta overexpression: 39 tumours gave an expression level 1.5-5 times, five tumours 10-20 times, and four tumours more than 20 times relative to normal mucosa. However, the general level of PPARdelta mRNA in rectal cancer tissues is not statistically different from normal mucosa.


CONCLUSIONS
The expression of PPARdelta gene in rectal cancers is not statistically different from normal mucosa.
Yang
L
L
Department of General Surgery and Division of Digestive Surgery and Organ-Microcirculation, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Zhou
Z-G
ZG
Luo
H-Z
HZ
Zhou
B
B
Xia
Q-J
QJ
Tian
C
C
D014408:Biomarkers, Tumor / Q000032:analysis*; D016022:Case-Control Studies; D002681:China; D003199:Computer Systems; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry; D047494:PPAR delta / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D012004:Rectal Neoplasms / Q000737:chemistry* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D047494:PPAR delta; D012333:RNA, Messenger