[Rapid separation and determination of enterotoxigenic E. coli by capillary zone electrophoresis].
Rapid separation and determination of enterotoxigenic E. coli K88, K99 and 987P with intact cells using capillary zone electrophoresis (CZE) are described. The CZE was performed in a running buffer of 0.05 mol/L Na2CO3-NaHCO3 (pH 9.9), under applied voltage of 14.1 kV and detected by an on-line UV at 210 nm. Results show that K88, K99 and 987P were separated completely. Moreover, each strain presented a characteristic peak and a reproducible retention time (RSD < or = 0.9%). The successful analysis of enterotoxigenic E. coli in the excrement of diarrhea piglets is also discussed. K88 and K99 were detected in the excrement of diarrhea piglets born in 5 d - 6 d, and 30 d - 35 d respectively, and nothing was detected in the excrement of diarrhea piglets born in about 60 d.
Chen
Ping
P
Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China. chen77ping@163.com
Li
Ren-Kuan
RK
Xu
Xiao-Hua
XH
Rao
Ping-Fan
PF
D000818:Animals; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D019075:Electrophoresis, Capillary / Q000379:methods*; D054307:Enterotoxigenic Escherichia coli / Q000737:chemistry* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*