Novel three-dimensional organoid model for evaluation of the interaction of uropathogenic Escherichia coli with terminally differentiated human urothelial cells.
Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced alpha-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.
Smith
Yarery C
YC
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Rm. B4052, 4301 Jones Bridge Rd., Bethesda, MD 20814-4799, USA.
Grande
Kerian K
KK
Rasmussen
Susan B
SB
O'Brien
Alison D
AD
D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D009940:Organoids / Q000382:microbiology / Q000473:pathology* / Q000648:ultrastructure; D019459:Urothelium / Q000382:microbiology* / Q000473:pathology* / Q000648:ultrastructure; D018474:Weightlessness Simulation