title: Evaluation of anti-stress nutrients in the endothelial cells with fluorescence indicator.
abstract: Oxidative stress has emerged as an important pathogenic factor in the development of long-term complications, such as hypertension, atherosclerosis, nephropathy, and cancer. Taking many antioxidants from natural food may be effective to prevent us from those diseases. We have attempted to evaluate the effect of improvement by dietary antioxidants on the endothelial dysfunction induced by hyperglycemia. Fluorescence indicators for reactive oxygen species and nitric oxide were employed to the evaluation. The combination of those fluorescence indicators could be powerful tool to evaluate the effect of anti-stress nutrients on both oxidative stress and endothelial dysfunction.
authors.affiliation: Department of Clinical Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan.
authors.forename: Kunitaka
authors.initials: K
authors.lastname: Nashiki
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Taketani
authors.forename: Emi
authors.initials: E
authors.lastname: Shuto
authors.forename: Rieko
authors.initials: R
authors.lastname: Tanaka
authors.forename: Ayako
authors.initials: A
authors.lastname: Tanimura
authors.forename: Naoki
authors.initials: N
authors.lastname: Sawada
authors.forename: Tadatoshi
authors.initials: T
authors.lastname: Sato
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Takei
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Arai
authors.forename: Hironori
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Nagakatsu
authors.initials: N
authors.lastname: Harada
authors.forename: Eiji
authors.initials: E
authors.lastname: Takeda
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D001013:Aorta, Thoracic / Q000166:cytology; D002417:Cattle; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D005456:Fluorescent Dyes; D005947:Glucose / Q000494:pharmacology*; D006943:Hyperglycemia / Q000378:metabolism; D018613:Microscopy, Confocal; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D005947:Glucose