title: Evaluation of the Bactec microbial detection system for culturing miscellaneous sterile body fluids.
abstract: This study sought to evaluate the efficacy of the Bactec blood culture system for culturing sterile body fluids, in comparison with traditional culture methods. A total of 906 specimens were cultured using both conventional media and the Bactec detection system. Differences in numbers of isolates determined by each system were compared. Clinically significant microorganisms were isolated from 15.3% (139) of 906 specimens by both the Bactec system and conventional culture, whereas for 8.8% (80) of the specimens a positive culture was obtained by Bactec only. Of the 80 specimens in which growth was detected only in the Bactec blood culture system, 11.0% were comprised of cerebrospinal fluid, 9.9% peritoneal fluid, 4.9% pleural fluid, 4.5% synovial fluid and 9.1% bone marrow materials. Strains of Brucella melitensis, Neisseria meningitidis, Pseudomonas fluorescens and Rothia dentocariosa were cultured only by the Bactec system. In conclusion, the Bactec blood culture system might be advantageous for isolation of fastidious microorganisms such as Brucella, especially from cerebrospinal and peritoneal fluid specimens.
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Medical School of Suleyman Demirel University, Isparta, Turkey. fzeynep@med.sdu.edu.tr
authors.forename: Fusun Zeynep
authors.initials: FZ
authors.lastname: Akcam
authors.forename: Guler
authors.initials: G
authors.lastname: Yayli
authors.forename: Ersin
authors.initials: E
authors.lastname: Uskun
authors.forename: Onur
authors.initials: O
authors.lastname: Kaya
authors.forename: Canan
authors.initials: C
authors.lastname: Demir
mesh_terms: D001419:Bacteria; D001424:Bacterial Infections / Q000175:diagnosis; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood; D001826:Body Fluids / Q000382:microbiology*; D001853:Bone Marrow / Q000382:microbiology; D003470:Culture Media; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media; D011933:Reagent Kits, Diagnostic