title: Use of a zona laser treatment system in assisted hatching: optimal laser utilization parameters.
abstract: OBJECTIVE
  This study aimed to establish the benefit of laser micromanipulation of the zona pellucida as a method of assisted hatching and to determine the optimal technical settings.
  
  
  METHODS
  Prospective pilot study.
  
  
  METHODS
  University teaching hospital.
  
  
  METHODS
  Six- to 8-week-old F1 female mice.
  
  
  METHODS
  Mouse embryos were treated with a laser (Hamilton Thorne Research) using both research and preset clinical settings. The research option allows the user to alter pulse duration, number of pulses, and beam intensity. In this study, laser intensity and duration, as well as the depth and number of disruptions, were altered.
  
  
  METHODS
  Embryos were scored after treatment as either arrested blastocysts, arrested during hatching, hatched, or degenerate.
  
  
  RESULTS
  The treatment where the laser intensity was halved but the number of laser pulses used was doubled was the most beneficial treatment. Zona thinning was not effective.
  
  
  CONCLUSIONS
  The study showed that laser-assisted hatching can safely and significantly increase the percentage of embryos reaching the hatched blastocyst stage. A proportionately sized hole in a human embryo may have the same beneficial effect as has been shown in this study.
authors.affiliation: Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Stellenbosch University, Tygerberg Hospital, Cape Town, South Africa. gmt@sun.ac.za
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Tinney
authors.forename: Marie-Lena
authors.initials: ML
authors.lastname: Windt
authors.forename: Thinus F
authors.initials: TF
authors.lastname: Kruger
authors.forename: Carl J
authors.initials: CJ
authors.lastname: Lombard
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D005260:Female; D007834:Lasers; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008846:Micromanipulation / Q000295:instrumentation*; D023421:Models, Animal; D010865:Pilot Projects; D011247:Pregnancy; D027724:Reproductive Techniques, Assisted / Q000295:instrumentation*; D015044:Zona Pellucida / Q000502:physiology*