title: Polarized subsets of human T-helper cells induce distinct patterns of chemokine production by normal and systemic sclerosis dermal fibroblasts.
abstract: The role of fibroblasts in inflammatory processes and their cross-talk with T cells is increasingly being recognized. Our aim was to explore the capacity of dermal fibroblasts to produce inflammatory chemokines potentially involved in fibrosis occurring in response to contact with polarized human T cells. Our findings indicate that the program of chemokine production by fibroblasts is differentially regulated depending on the T-helper (Th) cell subset used to activate them. Thus, Th1 and Th2 cells preferentially induced production of IFN-gamma inducible protein (IP)-10 and IL-8, respectively, whereas monocyte chemoattractant protein (MCP)-1 was equally induced by both subsets at mRNA and protein levels. Neutralization experiments indicated that membrane-associated tumour necrosis factor-alpha and IL-1 played a major role in the induction of IL-8 and MCP-1 by Th1 and Th2 cells, whereas membrane-associated IFN-gamma (present only in Th1 cells) was responsible, at least in part, for the lower IL-8 and higher IP-10 production induced by Th1 cells. The contributions of tumour necrosis factor-alpha, IL-1 and IFN-alpha were confirmed when fibroblasts were cultured separated in a semipermeable membrane from living T cells activated by CD3 cross-linking. We observed further differences when we explored signal transduction pathway usage in fibroblasts. Pharmacological inhibition of c-Jun N-terminal kinase and nuclear factor-kappaB resulted in inhibition of IL-8 mRNA transcription induced by Th1 cells but not that by Th2 cells, whereas inhibition of MEK/ERK (mitogen-activated protein kinase of extracellular signal-regulated kinase/extracellular signal-regulated kinase) and nuclear factor-kappaB resulted in inhibition of MCP-1 mRNA induced by Th2 but not by Th1 cells. Finally, no distinct differences in chemokine production were observed when the responses to T cell contact or to prototypic Th1 and Th2 cytokines were examined in systemic sclerosis versus normal fibroblasts. These findings indicate that fibroblasts have the potential to participate in shaping the inflammatory response through the activation of flexible programs of chemokine production that depend on the Th subset eliciting their response.
authors.affiliation: Immunology and Allergy, Geneva University Hospital, Geneva School of Medicine, Rue Micheli-du-Crest, 24, 1211 Geneva 14, Switzerland. chizzolini@medecine.unige.ch
authors.forename: Carlo
authors.initials: C
authors.lastname: Chizzolini
authors.forename: Yann
authors.initials: Y
authors.lastname: Parel
authors.forename: Agneta
authors.initials: A
authors.lastname: Scheja
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Dayer
mesh_terms: D001706:Biopsy; D002462:Cell Membrane / Q000276:immunology; D018925:Chemokines / Q000235:genetics*; D002999:Clone Cells; D018920:Coculture Techniques; D016207:Cytokines / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000503:physiopathology; D008297:Male; D012313:RNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D012016:Reference Values; D012595:Scleroderma, Systemic / Q000276:immunology* / Q000503:physiopathology; D012867:Skin / Q000276:immunology* / Q000473:pathology; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D018925:Chemokines; D016207:Cytokines; D016212:Transforming Growth Factor beta; D012313:RNA