title: Mitochondrial DNA diagnosis for taeniasis and cysticercosis.
abstract: Molecular diagnosis for taeniasis and cysticercosis in humans on the basis of mitochondrial DNA analysis was reviewed. Development and application of three different methods, including restriction fragment length polymorphism analysis, base excision sequence scanning thymine-base analysis and multiplex PCR, were described. Moreover, molecular diagnosis of cysticerci found in specimens submitted for histopathology and the molecular detection of taeniasis using copro-DNA were discussed.
authors.affiliation: Department of Parasitology, Asahikawa Medical College, Japan. hyamasak@asahikawa-med.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasaki
authors.forename: Minoru
authors.initials: M
authors.lastname: Nakao
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakaya
authors.forename: Marcello Otake
authors.initials: MO
authors.lastname: Sato
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000818:Animals; D001483:Base Sequence; D051545:Cyclooxygenase 1 / Q000737:chemistry / Q000235:genetics; D003551:Cysticercosis / Q000175:diagnosis* / Q000469:parasitology; D018509:DNA, Helminth / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis*; D014644:Genetic Variation; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013621:Taenia / Q000145:classification / Q000235:genetics / Q000254:growth & development; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D051545:Cyclooxygenase 1