title: Disruption of sucA, which encodes the E1 subunit of alpha-ketoglutarate dehydrogenase, affects the survival of Nitrosomonas europaea in stationary phase.
abstract: Although Nitrosomonas europaea lacks measurable alpha-ketoglutarate dehydrogenase activity, the recent completion of the genome sequence revealed the presence of the genes encoding the enzyme. A knockout mutation was created in the sucA gene encoding the E1 subunit. Compared to wild-type cells, the mutant strain showed an accelerated loss of ammonia monooxygenase and hydroxylamine oxidoreductase activities upon entering stationary phase. In addition, unlike wild-type cells, the mutant strain showed a marked lag in the ability to resume growth in response to pH adjustments in late stationary phase.
authors.affiliation: Department of Botany and Plant Pathology, Oregon State University, 2082 Cordley, Corvallis, OR 97331-2902, USA.
authors.forename: Norman G
authors.initials: NG
authors.lastname: Hommes
authors.forename: Elizabeth G
authors.initials: EG
authors.lastname: Kurth
authors.forename: Luis A
authors.initials: LA
authors.lastname: Sayavedra-Soto
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Arp
mesh_terms: D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D006863:Hydrogen-Ion Concentration; D007655:Ketoglutarate Dehydrogenase Complex / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D043370:Nitrosomonas europaea / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D010101:Oxygen Consumption; D016133:Polymerase Chain Reaction
chemical_list: D007655:Ketoglutarate Dehydrogenase Complex