Selective apoptotic behaviour of bovine herpesvirus 1 in an epithelial-like microenvironment.
Apoptosis seems to play an important role in the pathogenic profile of bovine herpesvirus 1 (BHV-1) infection. Nitric oxide (NO) is also important as a signal molecule. In this study, apoptosis was selectively induced in HEp-2 cells in the early stage [1-3 h postinfection (PI)] of BHV-1 multiplication, and this apoptotic process was realised through the caspase-8, and partially through the caspase-3, pathway. BHV-1 infection inhibited staurosporine- (SS-) induced apoptosis only if the SS was added at 6 h PI. The results of this study showed that the 'NO-apoptosis' relation was realised through the caspase-8 pathway ('outer membrane receptor' pathway) at a later stage of infection in apoptosis induced by BHV-1 + SS. Our previous report (Yazici et al., 2004) and this study together showed that BHV-1 might induce and inhibit cell-type-specific pathways of apoptosis.
Baskin
Yasemin
Y
Oncology Institute, School of Medicine, Dokuz Eylul University, Inciralti, Izmir, Turkey.
Yazici
Z
Z
Baskin
H
H
Ozkul
A
A
Olgun
Nur
N
Bahar
I H
IH
D017209:Apoptosis / Q000187:drug effects; D061945:Caspase Inhibitors; D045744:Cell Line, Tumor; D004848:Epithelium / Q000821:virology*; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology; D014779:Virus Replication
D061945:Caspase Inhibitors; D009569:Nitric Oxide; D019311:Staurosporine