Effects of flow cytometric analysis and cell sorting on photosynthetic carbon uptake by phytoplankton in cultures and from natural populations.
Photosynthetic rates of phytoplankton were significantly lower after analysis by flow cytometry (FCM) than before. Exposure to the laser beam during the sorting process caused significant physiological damage. The cellular content of a radiolabel accumulated prior to FCM was not affected by FCM. Although it may not be possible to use FCM to preconcentrate cells for further physiological studies, samples may be incubated with stable or radioactive isotopes and then analyzed by FCM.
Rivkin
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RB
Horn Point Environmental Laboratories, Center for Environmental and Estuarine Studies, University of Maryland, Cambridge, Maryland 21613, and Bigelow Laboratory for Ocean Sciences, McKown Point, West Boothbay Harbor, Maine 04575.
Phinney
D A
DA
Yentsch
C M
CM