In sacco studies of conjugated linoleic acid production from various oils in the rumen of sheep.
In this experiment sunflower oil, soybean oil and fish oil were incubated in rumen-fistulated adult ewes (n = 5) to study conjugated linoleic acid (CLA) production in the rumen. The individual oils were incubated in nylon bags in the rumen on perlite carrier (40% oil, 60% carrier) over a period of 2, 4, 6, 8, 10 and 12 h for all treatments. During the incubation of each oil primarily the formation of the cis-9, trans-11 isomer of CLA could be observed. Both sunflower and soybean oils showed similar changes in the rumen. After the incubation of these two vegetable oils the proportion of linoleic acid decreased significantly as the duration of incubation increased in the rumen. These changes were accompanied by a significant increase in the amount of cis-9, trans-11 CLA. However, in the case of sunflower oil the rate of formation of the cis-9, trans-11 CLA isomer was significantly higher after the different incubation times as compared to soybean oil. Much lower amounts of CLA were formed when fish oil was incubated in the rumen. The level of cis-9, trans-11 isomer produced during these treatments was 10% less than the amount obtained with the other two oils of vegetable origin. Besides the cis-9, trans-11 isomer, trans-10, cis-12 CLA could also be detected during the incubation of the different oils in the rumen. However, the level of this isomer was low and did not show consistent differences among the treatments. The results of this experiment indicate that the fatty acid composition of the oils and the duration of incubation collectively determine the amount of CLA produced in the first compartment of the forestomach of ruminants.
Szölloskei
G
G
Department of Animal Physiology and Animal Nutrition, Georgikon Faculty of Agriculture, University of Veszprém, Hungary.
Wagner
L
L
Németh
Stefánia
S
Husvéth
F
F
D000818:Animals; D005260:Female; D005395:Fish Oils / Q000378:metabolism* / Q000494:pharmacology; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis*; D010938:Plant Oils / Q000378:metabolism* / Q000494:pharmacology; D012417:Rumen / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*; D013024:Soybean Oil / Q000378:metabolism* / Q000494:pharmacology; D000074242:Sunflower Oil; D013997:Time Factors
D005395:Fish Oils; D044243:Linoleic Acids, Conjugated; D010938:Plant Oils; D000074242:Sunflower Oil; D013024:Soybean Oil