Bone surface and whole bone as biomarkers for acute fluoride exposure.
This study compares fluoride concentrations ([F]) in surface and whole bone for up to 27 days following an acute oral dose of F. Four groups of rats received single oral F dose (50 mg/kg body weight), and the control group received deionized water (n = 10/group). Groups were euthanized at 1, 3, 9, or 27 days after F administration. Plasma and femurs were collected. F on the femur surface was removed from a circular area (4.52 mm(2)) by immersion in 0.5M HCl for 15 s. The solution was buffered with total ionic strength adjustment buffer and analyzed with an electrode. The subjacent bone was sectioned and ashed at 600 degrees C. Ash and plasma were analyzed for F with the electrode following hexamethyldisiloxane-facilitated diffusion. Data were analyzed by Kruskall-Wallis and Dunn's test and by linear regression (p < 0.05). Peak plasma and bone surface [F] occurred on day 1 (0.26 +/- 0.14 microg/mL and 1801 +/- 888 microg/g, respectively). Bone surface [F] at 3, 9, and 27 were not statistically different from control. A significant increase in whole bone [F] was observed 3 days after F administration and the [F] remained relatively constant thereafter. The mean (+/- SD) surface/whole bone [F] ratios for the control and F groups were 2.45 +/- 0.98, 3.92 +/- 1.32, 1.61 +/- 0.82, 1.73 +/- 0.39, and 1.09 +/- 0.28, respectively. Plasma and bone surface [F]s were positively correlated (r = 0.74). Thus, bone surface was found to be a suitable biomarker for acute, sublethal F exposure 1 day after F administration. Whole bone [F] were significantly increased at 3, 9, and 27 days after F administration.
Buzalaf
Marília Afonso Rabelo
MA
Departamento de Ciências Biológicas, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Alameda Octávio Pinheiro Brisolla 9-75, 17012-901, Bauru, SP, Brazil. mbuzalaf@fob.usp.br
Caroselli
Elide Escolástico
EE
de Carvalho
Juliane Guimarães
JG
de Oliveira
Rodrigo Cardoso
RC
da Silva Cardoso
Vanessa Eid
VE
Whitford
Gary Milton
GM
D000818:Animals; D015415:Biomarkers; D001842:Bone and Bones / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods*; D005459:Fluorides / Q000032:analysis* / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D005459:Fluorides