title: Detection of mRNA in streptomyces cells by whole-cell hybridization with digoxigenin-labeled probes.
abstract: Detection of mRNA of the thiostrepton resistance gene (tsr) harbored by plasmid pIJ673 in Streptomyces violacelatus was achieved by whole-cell hybridization with digoxigenin-labeled in vitro transcripts followed by an antibody-alkaline phosphatase detection of the digoxigenin reporter molecule. Prior to hybridization, the cells had to be permeabilized by lysozyme, the detergent Nonidet P-40, and toluene. The permeability of the S. violacelatus cells for probes and the antibody-alkaline phosphatase conjugate was demonstrated by hybridization with digoxigenin-labeled, 16S rRNA-targeted oligonucleotides.
authors.affiliation: Institute of Terrestrial Ecology, Soil Biology, Eidgenössische Technische Hochschule Zürich, Grabenstrasse 3, CH-8952 Schlieren, Switzerland, and Lehrstuhl für Mikrobiologie, Technische Universität München, Arcisstrasse 21, D-8000 Munich 2, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Hahn
authors.forename: R I
authors.initials: RI
authors.lastname: Amann
authors.forename: J
authors.initials: J
authors.lastname: Zeyer