Wood degradation by white rot fungi: cytochemical studies using lignin peroxidase-immunoglobulin-gold complexes.
Using an anti-lignin peroxidase antiserum-protein A-gold complex, we found lignin peroxidase mainly intracellularly in several white rot fungi colonizing sawdust under laboratory conditions. This enzyme was also present in fungi found in naturally decayed wood. However, in all cases, lignin peroxidase was located mainly inside the fungal cells. Labeled lignin peroxidase did not bind to the lignocellulosic samples tested, with the exception of poplar milled-wood lignin. These results are discussed in relation to the role of lignin peroxidase during wood degradation.
Garcia
S
S
Unité de Mycologie and Station Centrale de Microscopie Electronique, Institut Pasteur, 75724 Paris Cedex 15, France, and Institut für Biotechnologie, Eidgenosische Technische Holzschule-Hönggerberg, CH-8093 Zurich, Switzerland.
Latge
J P
JP
Prevost
M C
MC
Leisola
M
M