title: Safe Self-contained Carbon Dioxide-Hydrogen Anaerobic System.
abstract: An anaerobic system is described which allows the microbiologist or hospital technician ease of operation not previously possible with other systems. Gas cylinders, vacuum pumps, valves, and gauges have been eliminated. A new anaerobic lid was developed that is fitted only with a snap-in rubber gasket, a double stainless-steel gauze flash arrestor, and a catalyst holder. The holder contains a palladium pellet catalyst which is active at room temperature and requires no heat for activation. This system was made specifically for use with a disposable hydrogen-carbon dioxide generator and a disposable methylene blue anaerobic indicator. In addition to ease of operation, this jar incorporates unique safety features which eliminate the possibility of a laboratory explosion. An oxygen-free atmosphere composed primarily of nitrogen and carbon dioxide was quickly achieved within the jar to insure maximal growth.
authors.affiliation: Becton, Dickinson and Co. and Baltimore Biological Laboratory, Baltimore, Maryland.
authors.forename: J H
authors.initials: JH
authors.lastname: Brewer
authors.forename: D L
authors.initials: DL
authors.lastname: Allgeier