title: Two types of precursor cells in a multipotential hematopoietic cell line.
abstract: The biochemistry of early stages of hematopoietic differentiation is difficult to study because only relatively small numbers of precursor cells are available. The murine EML cell line is a multipotential cell line that can be used to model some of these steps. We found that the lineage- EML precursor cells can be separated into two populations based on cell surface markers including CD34. Both populations contain similar levels of stem cell factor (SCF) receptor (c-Kit) but only the CD34+ population shows a growth response when treated with SCF. Conversely, the CD34- population will grow in the presence of the cytokine IL-3. The human beta-globin locus control region hypersensitive site 2 plays different roles on beta-globin transcription in the CD34+ and CD34- populations. The two populations are present in about equal amounts in culture, and the CD34+ population rapidly regenerates the mixed population when grown in the presence of SCF. We suggest that this system may mimic a normal developmental transition in hematopoiesis.
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, CT 06519, USA.
authors.forename: Zhi-jia
authors.initials: ZJ
authors.lastname: Ye
authors.forename: Yuval
authors.initials: Y
authors.lastname: Kluger
authors.forename: Zheng
authors.initials: Z
authors.lastname: Lian
authors.forename: Sherman M
authors.initials: SM
authors.lastname: Weissman
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics; D005914:Globins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D011485:Protein Binding; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D016185:Receptors, Interleukin-3 / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D016185:Receptors, Interleukin-3; D014157:Transcription Factors; D005914:Globins; D019009:Proto-Oncogene Proteins c-kit