Genetic integrity of somaclonal variants in tea (Camellia sinensis (L.) O Kuntze) as revealed by inter simple sequence repeats.
Adoption of inter simple sequence repeats (ISSR) technique to analyze the genetic variability of somatic embryo derived tea plants was evaluated. Morphological characterisation of the field grown plants revealed no identical character aligning with the parent, UPASI-10. Out of 40 primers, 15 exhibited concurrent polymorphism were selected for the study. Genetic variability of somaclones derived from single line cotyledonary culture ranged from 33.0 to 55.0%. A unique fragment of 1.2Kb was visible in majority of the accessions whereas the fragments below the length of 0.6Kb were noticed only in 50% of the variants. Out of 120 interactions attempted using Pearson's coefficient correlation, only 9.2% of somaclones exhibited significant similarity at genetic level. Dendrogram constructed based on simple matching coefficient revealed a distance of 2.257-3.317 between the final clusters. This strengthens the existence of wide genetic variation among the somaclones.
Thomas
Jibu
J
Plant Physiology and Biotechnology, UPASI Tea Research Institute, Nirar Dam BPO, Valparai, Coimbatore District, Tamil Nadu 642 127, India. jibutoms@rediffmail.com
Vijayan
Deepu
D
Joshi
Sarvottam D
SD
Joseph Lopez
S
S
Raj Kumar
R
R
D028241:Camellia sinensis / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis / Q000379:methods; D018744:DNA, Plant / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
D018744:DNA, Plant