title: Structure and assembly of epiglucan, the extracellular (1-->3;1-->6)-beta-glucan produced by the fungus Epicoccum nigrum strain F19.
abstract: In a previous article [Carbohydr. Res.2001, 331, 163-171] two different structures for the possible modular repeating unit of the extracellular beta-glucan, epiglucan produced by the fungus Epicoccum nigrum strain F19 were proposed. Clarifying which was the more likely one was considered essential before attempts were made to understand how epiglucan was assembled by this fungus. Data from Smith degradation analyses of epiglucan were consistent with the repeating unit of structure I, where single glucosyl residues are attached by (1-->6)-beta-linkages to two out of every three glucosyl residues in the (1-->3)-beta-linked glucan backbone. Repeated Smith degradations of 14C-glucose labelled epiglucan showed that chain elongation occurred from its non-reducing end. Side chain insertion into the growing glucan was followed by analysis of real time incorporation of 13C-glucose into epiglucan by 13C NMR, and 14C-glucose by enzymic digestion of the synthesised 14C-epiglucan. All data obtained were consistent with the view that single (1-->6)-beta-linked glucosyl side residues are inserted simultaneously as the glucan backbone elongates.
authors.affiliation: School of Agriculture and Wine, The University of Adelaide, PMB1, Glen Osmond, SA 5064, Australia.
authors.forename: Frank
authors.initials: F
authors.lastname: Schmid
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Stone
authors.forename: Robert T C
authors.initials: RT
authors.lastname: Brownlee
authors.forename: Barbara M
authors.initials: BM
authors.lastname: McDougall
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Seviour
mesh_terms: D001203:Ascomycota / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D043326:Glucan 1,3-beta-Glucosidase / Q000378:metabolism; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D013055:Spectrophotometry, Infrared; D047071:beta-Glucans / Q000737:chemistry / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D005936:Glucans; D047071:beta-Glucans; C033671:epiglucan; D043326:Glucan 1,3-beta-Glucosidase