Quantification of hemokinin-1 peptide production and secretion from mouse B cells.
Hemokinin-1 is a recent addition to the family of mammalian tachykinins and is thought to play an important role in B cell and T cell lymphopoiesis. The mRNA coding for this peptide was expressed in some B lymphocyte cell lines including 70Z/3.12, ABE-8.1/2, and RAW8.1 cells, suggesting the possibility that hemokinin-1 may function in an autocrine or paracrine manner in these cells. Therefore, we quantified secretion of this peptide from the 70Z/3.12 cell line expressing hemokinin-1 mRNA. Despite a sensitive radioimmunoassay, we were surprised to find that hemokinin-1 secretion from confluent cells was below the level of detection of this assay. Furthermore, cell lysates routinely demonstrated a low or undetectable immunoreactive peptide. Collectively these studies show a limited production of hemokinin-1 peptide by transformed B cells.
Nelson
Daniel A
DA
Department of Biology, 9201 University City Boulevard, University of North Carolina at Charlotte, Charlotte, NC 28223, USA. danelson@email.uncc.edu
Bost
Kenneth L
KL
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D051379:Mice; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011863:Radioimmunoassay; D013373:Substance P / Q000032:analysis / Q000235:genetics; D015320:Tachykinins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics
D018076:DNA, Complementary; D011498:Protein Precursors; D012333:RNA, Messenger; C423233:Tac4 protein, mouse; D015320:Tachykinins; D013373:Substance P