title: Identification of frankia strains by direct DNA hybridization of crushed nodules.
abstract: A hybridization procedure was developed to identify Frankia strains inside actinorhizae by direct probing of crushed root nodules. The probe consisted of an indigenous cryptic plasmid. This well-conserved, 8-kilobase plasmid was detected in Frankia isolates that were very close taxonomically (they possessed a very high DNA sequence homology). The probe did not hybridize to the DNA of Frankia isolates which did not carry the plasmid. Endophyte DNA was extracted by a modification of a technique originally developed for the detection of plasmids in Frankia isolates. The hybridization procedure applied to nodules collected in a stand of alder permitted determination of a distribution map of the plasmid-bearing Frankia strains.
authors.affiliation: Laboratoire de Biologie des Sols, U.A. Centre National de la Recherche Scientifique 697, Bâtiment 741, Université Lyon 1, Villeurbanne F-69622 Cedex, and Station d' Amélioration des Arbres Forestiers, Institut National de la Recherche Agronomique, Orléans, Ardon F-45160 Olivet, France.
authors.forename: P
authors.initials: P
authors.lastname: Simonet
authors.forename: N T
authors.initials: NT
authors.lastname: Le
authors.forename: E T
authors.initials: ET
authors.lastname: Du Cros
authors.forename: R
authors.initials: R
authors.lastname: Bardin