title: Effect of Surface-Active Pseudomonas spp. on Leaf Wettability.
abstract: Different strains of Pseudomonas putida and P. fluorescens isolated from the rhizosphere and phyllosphere were tested for surface activity in droplet cultures on polystyrene. Droplets of 6 of the 12 wild types tested spread over the surface during incubation, and these strains were considered surface active; strains not showing this reaction were considered non-surface active. Similar reactions were observed on pieces of wheat leaves. Supernatants from centrifuged broth cultures behaved like droplets of suspensions in broth; exposure to 100 degrees C destroyed the activity. Average contact angles of the supernatants of surface-active and non-surface-active strains on polystyrene were 24 degrees and 72 degrees , respectively. The minimal surface tension of supernatants of the surface-active strains was about 46 mN/m, whereas that of the non-surface-active strains was 64 mN/m (estimations from Zisman plots). After 6 days of incubation, wheat flag leaves sprayed with a dilute suspension of a surface-active strain of P. putida (WCS 358RR) showed a significant increase in leaf wettability, which was determined by contact angle measurements. Increasing the initial concentration of bacteria and the amount of nutrients in the inoculum sprayed on leaves reduced the contact angles from 138 degrees on leaves treated with antibiotics (control) to 43 degrees on leaves treated with surface-active bacteria. A closely related strain with no surface activity on polystyrene did not affect leaf wettability, although it was present in densities similar to those of the surface-active strain. Nutrients alone could occasionally also increase leaf wettability, apparently by stimulating naturally occurring surface-active bacteria. When estimating densities of Pseudomonas spp. underneath droplets with low contact angles, it appeared that populations on leaves treated with a surface-active strain could vary from about 10 to 10 CFU cm, suggesting that the surface effect may be prolonged after a decline of the population. The possible ecological implications are discussed.
authors.affiliation: Willie Commelin Scholten Phytopathological Laboratory, Javalaan 20, 3742 CP Baarn, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Bunster
authors.forename: N J
authors.initials: NJ
authors.lastname: Fokkema
authors.forename: B
authors.initials: B
authors.lastname: Schippers