A serum-free medium for colony growth and hyaluronic acid production by Streptococcus zooepidemicus NJUST01.
A hyaluronic acid (HA)-producing strain, Streptococcus zooepidemicus NJUST01, can grow in a serum-free agar medium, with starch as exclusive carbon source, but not glucose, sucrose, dextrine, xylose, or lactose. In this starch medium, the strain NJUST01 reproduced successively at 37 degrees C for 60 generations, with no obvious variation on morphology and physiology, but colonies of the strain after 60th generation could not produce a clear hemolytic zone on sheep blood agar plates. Hyaluronic acid production by the strain NJUST01 was analyzed relative to the starch medium. Employing a multifactor cross experiment, an optimum medium revealed for hyaluronic acid production was composed of 5% starch, 0.3% glucose, 0.5% peptone, 0.15% MgSO4, and 2.0% K2HPO4. The amount of HA 6.7 g/l was obtained in batch fermentation on a rotary shaker at 37 degrees C, 220 rpm for 36 h.
Zhang
Jianfa
J
State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing, 210093, People's Republic of China. jfzhang@mail.njust.edu.cn.|Department of Biochemical Engineering, Nanjing University of Science & Technology, Nanjing, 210094, People's Republic of China. jfzhang@mail.njust.edu.cn.
Ding
Xia
X
Department of Biochemical Engineering, Nanjing University of Science & Technology, Nanjing, 210094, People's Republic of China.
Yang
Liuyan
L
State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing, 210093, People's Republic of China.
Kong
Zhiming
Z
State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing, 210093, People's Republic of China.
D050260:Carbohydrate Metabolism; D003470:Culture Media / Q000737:chemistry; D016895:Culture Media, Serum-Free; D005285:Fermentation; D006460:Hemolysin Proteins / Q000096:biosynthesis; D006461:Hemolysis; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D018502:Streptococcus equi / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*
D003470:Culture Media; D016895:Culture Media, Serum-Free; D006460:Hemolysin Proteins; D006820:Hyaluronic Acid