title: Active and passive smoking and lifestyle determinants of 8-oxo-7,8-dihydro-2'-deoxyguanosine levels in human leukocyte DNA.
abstract: We investigated the effects of smoking and exposure to environmental tobacco smoke (ETS) on oxidative DNA damage by measuring 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) levels in DNA of leukocytes of healthy donors (30 smokers, 29 nonsmokers, and 28 ETS-exposed subjects). Nonsmokers had lower 8-oxodGuo levels compared with smokers (5.94 +/- 0.87 x 10(-6) and 19.85 +/- 4.75 x 10(-6) 2-deoxyguanosine, respectively, means +/- SE, P = 0.00007). Subjects exposed to ETS had higher mean value of 8-oxodGuo compared with nonsmokers (9.18 +/- 1.53 x 10(-6) 2-deoxyguanosine, mean +/- SE), nonsignificant by univariate analysis (P = 0.074). Multiregression analysis indicated that the increase of 8-oxodGuo levels induced by ETS was significant (P = 0.045) and that coffee and tea consumption reduced DNA oxidation (P = 0.0053). Oxidative leukocyte DNA damage was positively correlated with plasma cotinine levels in ETS-exposed subjects (r = 0.47, P < 0.01, n = 28) and was increased by age in nonsmokers and ETS-exposed subjects (P = 0.049). The results seem to confirm that ETS exposure is capable of inducing some oxidative DNA damage in circulating leukocytes and that coffee and tea consumption might partially protect against smoking-induced oxidation damage.
authors.affiliation: Department of Pharmacology, Viale Pieraccini 6, 50134 Florence, Italy. maura.lodovici@unifi.it
authors.forename: Maura
authors.initials: M
authors.lastname: Lodovici
authors.forename: Silvia
authors.initials: S
authors.lastname: Caldini
authors.forename: Cristina
authors.initials: C
authors.lastname: Luceri
authors.forename: Franco
authors.initials: F
authors.lastname: Bambi
authors.forename: Vieri
authors.initials: V
authors.lastname: Boddi
authors.forename: Piero
authors.initials: P
authors.lastname: Dolara
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D003069:Coffee; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D007558:Italy; D007962:Leukocytes / Q000737:chemistry*; D008019:Life Style; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis; D012907:Smoking / Q000097:blood*; D013662:Tea; D014028:Tobacco Smoke Pollution
chemical_list: D003069:Coffee; D013662:Tea; D014028:Tobacco Smoke Pollution; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine