Integrin alphaIIbbeta3 induces the adhesion and activation of mast cells through interaction with fibrinogen.
Integrin alphaIIb, a well-known marker of megakaryocyte-platelet lineage, has been recently recognized on hemopoietic progenitors. We now demonstrate that integrin alphaIIbbeta3 is highly expressed on mouse and human mast cells including mouse bone marrow-derived mast cells, peritoneal mast cells, and human cord blood-derived mast cells, and that its binding to extracellular matrix proteins leads to enhancement of biological functions of mast cells in concert with various stimuli. With exposure to various stimuli, including cross-linking of FcepsilonRI and stem cell factor, mast cells adhered to extracellular matrix proteins such as fibrinogen and von Willebrand factor in an integrin alphaIIbbeta3-dependent manner. In addition, the binding of mast cells to fibrinogen enhanced proliferation, cytokine production, and migration and induced uptake of soluble fibrinogen in response to stem cell factor stimulation, implicating integrin alphaIIbbeta3 in a variety of mast cell functions. In conclusion, mouse and human mast cells express functional integrin alphaIIbbeta3.
Oki
Toshihiko
T
Division of Cellular Therapy and Division of Hematopoietic Factors, Advanced Clinical Research Center, University of Tokyo, Japan.
Kitaura
Jiro
J
Eto
Koji
K
Lu
Yang
Y
Maeda-Yamamoto
Mari
M
Inagaki
Naoki
N
Nagai
Hiroichi
H
Yamanishi
Yoshinori
Y
Nakajima
Hideaki
H
Nakajina
Hideaki
H
Kumagai
Hidetoshi
H
Kitamura
Toshio
T
D000818:Animals; D002448:Cell Adhesion / Q000276:immunology*; D005340:Fibrinogen / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000096:biosynthesis* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D005340:Fibrinogen