title: Production of d-Aminoacylase from Alcaligenes denitrificans subsp. xylosoxydans MI-4.
abstract: A bacterial strain that produces d-aminoacylase was isolated from soil and identified as Alcaligenes denitrificans subsp. xylosoxydans MI-4. l-Aminoacylase activity in this strain was only 1 to 2% of d-aminoacylase activity. d-Aminoacylase was inducibly produced. N-Acetyl-dl-leucine was the best inducer, and the d-isomer had the ability to induce the enzyme. Enzymatic resolution of N-acetyl-dl-methionine with the crude enzyme was carried out, and the d/l ratio in the resolved methionine was approximately 100/7, suggesting that resolution with crude enzymes may become possible by removing small amounts of the contaminated l-form with l-amino acid oxidase.
authors.affiliation: Department of Environmental Chemistry and Engineering, Faculty of Engineering, Oita University, Oita 870-11, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Moriguchi
authors.forename: K
authors.initials: K
authors.lastname: Ideta