title: Detection of beta-Glucosidase Activity in Polyacrylamide Gels with Esculin as Substrate.
abstract: beta-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by beta-glucosidase action reacts with ferric ion to produce a black band, corresponding to the beta-glucosidase, against the transparent background.
authors.affiliation: Department of Microbiology, Seoul National University, Seoul, 151-742, Korea.
authors.forename: K S
authors.initials: KS
authors.lastname: Kwon
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: H G
authors.initials: HG
authors.lastname: Kang
authors.forename: Y C
authors.initials: YC
authors.lastname: Hah