title: Legionella pneumophila serogroup 1 isolates from cooling towers in Japan form a distinct genetic cluster.
abstract: Thirty-one epidemiologically unrelated Legionella pneumophila serogroup 1 isolates (10 from cooling towers, 10 from public spas and/or hot spring baths, and 11 from patients) were analyzed by pulsed-field gel electrophoresis (PFGE) and sequence-based typing (SBT) using 6 loci, flaA, pilE, asd, mip, mompS, and proA. The results of PFGE and SBT analysis indicated that all 10 isolates from cooling towers clustered into a unique type, which was distinct from strains of other environmental sources.
authors.affiliation: Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan. jmaekawa@nih.go.jp
authors.forename: Junko
authors.initials: J
authors.lastname: Amemura-Maekawa
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Kura
authors.forename: Bin
authors.initials: B
authors.lastname: Chang
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D004783:Environmental Microbiology; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D007564:Japan; D016952:Legionella pneumophila / Q000145:classification / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000175:diagnosis / Q000382:microbiology / Q000635:transmission; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011998:Recreation; D017422:Sequence Analysis, DNA / Q000379:methods; D012703:Serotyping; D014881:Water Supply
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial