Design, synthesis, and screening of biomimetic ligands for affinity chromatography.
Affinity chromatography is ideally suited to the purification of pharmaceutical proteins due to its unique bio-specificity characteristics. Tailor-made affinity ligands that represent a promising class of synthetic affinity ligands have been developed to target specific proteins and designed to mimic peptidal templates, natural biological recognition motifs, or complementary surface-exposed residues. These biomimetic ligands have been generated by a combination of rational design, combinatorial library synthesis, and subsequent screening of potential leads against target proteins. Small ligands based on a triazine scaffold also present exceptional selectivity and stability, which allows their use in harsh manufacturing environments.
Roque
Ana Cecília A
AC
Institute of Biotechnology, University of Cambridge, UK.
Gupta
Geeta
G
Lowe
Christopher R
CR
D032701:Biomimetics; D002846:Chromatography, Affinity / Q000379:methods*; D020650:Combinatorial Chemistry Techniques; D015195:Drug Design; D008024:Ligands; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D012984:Software; D013329:Structure-Activity Relationship; D014227:Triazines / Q000737:chemistry / Q000378:metabolism
D008024:Ligands; D011506:Proteins; D014227:Triazines