title: High Production of Thermostable beta-Galactosidase of Bacillus stearothermophilus in Bacillus subtilis.
abstract: By cloning the beta-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis, enzyme production was enhanced 50 times. beta-Galactosidase could be purified to 80% homogeneity by incubating the cell extract of B. subtilis at 70 degrees C for 15 min, followed by centrifugation to remove the denatured proteins. Because of its heat stability and ease of production, beta-galactosidase is suitable for application in industrial processes.
authors.affiliation: Department of Fermentation Technology, Osaka University, Yamada-oka, Suita-shi, Osaka 565, and Wakamoto Pharmaceutical Co., Ltd., Nihonbashimuromachi 1-8, Chuo-ku, Tokyo 103, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hirata
authors.forename: S
authors.initials: S
authors.lastname: Negoro
authors.forename: H
authors.initials: H
authors.lastname: Okada