Laser-induced fluorescence imaging and spectroscopy of GFP transgenic plants.
Green fluorescent protein (GFP) and other fluorescent protein bioreporters can be used to monitor transgenes in plants. GFP is a valuable marker for transgene presence and expression, but remote sensing instrumentation for stand-off detection has lagged behind fluorescent protein marker biotechnology. However, both biology and photonics are needed for the monitoring technology to be fully realized. In this paper, we describe laser-induced fluorescence imaging and laser-induced fluorescence spectroscopy of GFP-transgenic plants in ambient light towards the application of remote sensing of transgenic plants producing GFP.
Stewart
C Neal
CN
Department of Plant Sciences, University of Tennessee, Knoxville, Tennesssee 37996, USA. nealstewart@utk.edu
Millwood
Reginald J
RJ
Halfhill
Matthew D
MD
Ayalew
Mentewab
M
Cardoza
Vinitha
V
Kooshki
Mitra
M
Capelle
Gene A
GA
Kyle
Kevin R
KR
Piaseki
David
D
McCrum
Gregory
G
Di Benedetto
John
J
D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D007834:Lasers; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019076:Transgenes
D049452:Green Fluorescent Proteins