title: Tandem mass spectrometry of coprogen and deferoxamine hydroxamic siderophores.
abstract: Mechanisms of fragmentation of hydroxamic siderophores are proposed comparing deuterated and nondeuterated samples. Standard siderophores (e.g. deferoxamine and coprogen) were directly injected into both ion trap and linear quadrupole mass spectrometers with electrospray ionization (ESI). Four and two fragmentation steps were carried out for deferoxamine and coprogen (analyzed by positive and negative ESI, respectively). Deferoxamine cleavages occurred in both peptide and hydroxamic bonds while the coprogen fragmentation pattern is more elaborate, since it contains Fe(III) in its structure.
authors.affiliation: Laboratório de Cromatografia, Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador Sãocarlense, 400 São Carlos, SP 13566-590, Brasil.
authors.forename: Ana V C
authors.initials: AV
authors.lastname: Simionato
authors.forename: Gezimar D
authors.initials: GD
authors.lastname: de Souza
authors.forename: Edson
authors.initials: E
authors.lastname: Rodrigues-Filho
authors.forename: James
authors.initials: J
authors.lastname: Glick
authors.forename: Paul
authors.initials: P
authors.lastname: Vouros
authors.forename: Emanuel
authors.initials: E
authors.lastname: Carrilho
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D003676:Deferoxamine / Q000032:analysis*; D006877:Hydroxamic Acids / Q000032:analysis*; D017262:Siderophores / Q000032:analysis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D006877:Hydroxamic Acids; D017262:Siderophores; C010896:coprogen; D003676:Deferoxamine