HPLC determination of acetaminophen in saliva based on precolumn fluorescence derivatization with 12-(3,5-dichloro-2,4,6-triazinyl)benzo[d]benzo[1',2'-6,5]isoindolo[1,2-b][1,3]thiazolidine.
A high-performance liquid-chromatographic method for the determination of acetaminophen in saliva has been developed. This method is based on the precolumn derivatization of acetaminophen with 12-(3,5-dichloro-2,4,6-triazinyl)benzo[d]benzo[1',2'-6,5]isoindolo[1,2-b][1,3]thiazolidine, a new fluorescence derivatization reagent for phenolic compounds. The resulting derivative of acetaminophen is separated by isocratic elution on a reversed-phase column, and is fluorometrically detected at an emission wavelength of 560 nm with an excitation wavelength of 540 nm. The detection limit (signal-to-noise ratio = 3) was 0.1 microg/mL in saliva. The proposed method permits a highly sensitive and simple determination of acetaminophen in a small amount of saliva without any sample purification.
Fujino
Hiroyuki
H
PET Diagnostic Imaging Center, Kitakyusyu Medical Checkup Clinic, Japan.
Yoshida
Hideyuki
H
Nohta
Hitoshi
H
Yamaguchi
Masatoshi
M
D000082:Acetaminophen / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005453:Fluorescence; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry*; D053778:Thiazolidines
C508037:12-(3,5-dichloro-2,4,6-triazinyl)benzo(d)benzo(1',2'-6,5)isoindolo(1,2-b)(1,3)thiazolidine; D007211:Indoles; D054833:Isoindoles; D013844:Thiazoles; D053778:Thiazolidines; D000082:Acetaminophen