Gene transfer to trabecular meshwork endothelium via direct injection into the Schlemm canal and in vivo toxicity study.
OBJECTIVE
Our aim was to investigate the efficiency of adenoviral gene transfer via direct injection into the Schlemm canal ex vivo in human donor eyes and to examine the effect of human MMP-3 transgene expression in a rat model in vivo.


METHODS
A viscocanalostomy-like operation was performed and adenoviral vector encoding for MMP-3 and green fluorescent protein was injected into human Schlemm canal or rat anterior chamber.


RESULTS
Transgene expression was high in trabecular meshwork endothelium in human donor eyes. In vivo, adenovirus caused dose-dependent inflammation.


CONCLUSIONS
Direct injection of adenoviral vectors into the Schlemm canal has potential in glaucoma treatment.
Hudde
Tobias
T
Zentrum für Augenheilkunde, Abteilung für Erkrankungen des vorderen Augenabschnitts, Essen, Germany. tobias.hudde@uni-essen.de
Apitz
Janine
J
Bordes-Alonso
Rafael
R
Heise
Kerstin
K
Johnson
Kristian T M
KT
Steuhl
Klaus-Peter
KP
Geerling
Gerd
G
Pützer
Brigitte M
BM
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000867:Anterior Chamber / Q000201:enzymology / Q000821:virology; D015153:Blotting, Western; D002460:Cell Line; D004727:Endothelium / Q000201:enzymology* / Q000473:pathology; D004728:Endothelium, Corneal / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007267:Injections; D016850:Limbus Corneae / Q000201:enzymology / Q000821:virology; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014129:Trabecular Meshwork / Q000201:enzymology* / Q000473:pathology; D019076:Transgenes
D049452:Green Fluorescent Proteins; D019278:Matrix Metalloproteinase 3