title: A comparative study of the effects of hemin and bilirubin on bilateral renal ischemia reperfusion injury.
abstract: OBJECTIVE
  The aim of this study was to determine the effects of hemin, a heme oxygenase-1 inducer, and bilirubin on renal ischemia-reperfusion (I-R) injury.
  
  
  METHODS
  40 Wistar-Albino rats were allocated into six groups as follows: sham (S), bilirubin (B), hemin (H), ischemia/reperfusion (IR), IR + bilirubin (IRB) and IR + hemin (IRH). Conjugated bilirubin (20 mg.kg(-1) i.v.) was given to rats in groups B and IRB, and hemin (50 mg.kg(-1) i.p.) was given to rats in groups H and IRH just prior to reperfusion. Renal I-R was achieved by occluding the renal arteries bilaterally for 50 min. Following 6 h of reperfusion, blood was drawn to study BUN, creatinine and bilirubin, and tissue samples were harvested to determine the renal malonyldialdehyde and heme oxygenase-1 levels, and for histopathologic grading.
  
  
  RESULTS
  BUN, creatinine and malonyldialdehyde levels in group IRH were similar to controls whereas the results of groups IR and IRB were significantly higher (p < 0.01). There was a grade 2 damage in all I-R groups.
  
  
  CONCLUSIONS
  This study showed the preventive effect of hemin on renal ischemia reperfusion injury. Administration of exogenous bilirubin did not prevent the I-R injury.
authors.affiliation: Department of Pediatric Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey. billurd@yahoo.com
authors.forename: Billur
authors.initials: B
authors.lastname: Demirogullari
authors.forename: Gulsen
authors.initials: G
authors.lastname: Ekingen
authors.forename: Galip
authors.initials: G
authors.lastname: Guz
authors.forename: Neslihan
authors.initials: N
authors.lastname: Bukan
authors.forename: Ozlem
authors.initials: O
authors.lastname: Erdem
authors.forename: I Onur
authors.initials: IO
authors.lastname: Ozen
authors.forename: Leyla
authors.initials: L
authors.lastname: Memis
authors.forename: Sevki
authors.initials: S
authors.lastname: Sert
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000097:blood / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006427:Hemin / Q000494:pharmacology*; D007668:Kidney / Q000098:blood supply*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000187:drug effects; D015427:Reperfusion Injury / Q000503:physiopathology*
chemical_list: D006427:Hemin; D003404:Creatinine; D051547:Heme Oxygenase-1; D001663:Bilirubin