Contribution of a PerR-like regulator to the oxidative-stress response and virulence of Enterococcus faecalis.
PerR is one of the most important transcriptional regulators involved in the oxidative-stress response in Bacillus subtilis. Here, the homologous gene in Enterococcus faecalis, ranked among the leading causes of nosocomial infection, was characterized and analysed. Phenotype analysis showed that the perR mutant was significantly more resistant to H2O2 challenge (P < 0.05). Expression of eight genes with potential roles in the oxidative-stress response was determined in the wild-type and perR-mutant strains by real-time quantitative PCR. Surprisingly, low quantitative differences in the transcriptional activity of these genes in the mutant versus wild-type were observed. Likewise, this locus was not involved in survival within murine macrophages, but in the mouse peritonitis model, the perR mutant appeared less lethal than the JH2-2 wild-type strain. The combined results show that PerR affects E. faecalis virulence and that its implication in the transcriptional regulation in this bacterium deviates from the B. subtilis model.
Verneuil
Nicolas
N
Laboratoire de Microbiologie de l'Environnement, EA 956 soutenue par l'INRA, IRBA, Université de Caen, 14032 Caen Cedex, France.
Rincé
Alain
A
Laboratoire de Microbiologie de l'Environnement, EA 956 soutenue par l'INRA, IRBA, Université de Caen, 14032 Caen Cedex, France.
Sanguinetti
Maurizio
M
Institute of Microbiology, Catholic University of Sacred Heart, L. go F. Vito 1, 00168, Rome, Italy.
Posteraro
Brunella
B
Institute of Microbiology, Catholic University of Sacred Heart, L. go F. Vito 1, 00168, Rome, Italy.
Fadda
Giovanni
G
Institute of Microbiology, Catholic University of Sacred Heart, L. go F. Vito 1, 00168, Rome, Italy.
Auffray
Yanick
Y
Laboratoire de Microbiologie de l'Environnement, EA 956 soutenue par l'INRA, IRBA, Université de Caen, 14032 Caen Cedex, France.
Hartke
Axel
A
Laboratoire de Microbiologie de l'Environnement, EA 956 soutenue par l'INRA, IRBA, Université de Caen, 14032 Caen Cedex, France.
Giard
Jean-Christophe
JC
Laboratoire de Microbiologie de l'Environnement, EA 956 soutenue par l'INRA, IRBA, Université de Caen, 14032 Caen Cedex, France.
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D013293:Enterococcus faecalis / Q000472:pathogenicity* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014774:Virulence
D001426:Bacterial Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C122138:peroxide repressor proteins