title: A possible new bridge between innate and adaptive immunity: Are the anti-mitochondrial citrate synthase autoantibodies components of the natural antibody network?
abstract: Natural antibody (nAb) producing B-1 B cells are considered an intermediate stage of evolution between innate and adaptive immunity. nAbs are immunoglobulins that are produced without antigen priming. nAbs can recognize foreign targets and may serve in the first line of immune defense during an infection. Natural autoantibodies (nAAbs) present in the serum of both healthy humans and patients suffering from systemic autoimmune diseases recognize a set of evolutionarily conserved self-structures. Because of their endosymbiotic evolutionary origin, proteins compartmentalized into mitochondria represent an interesting transition from prokaryotic foreign (non-self) to essential (self) molecules. We investigated the possible overlap in recognized epitopes of innate and self-reactive nAbs and surveyed changes in physiological autoreactivity under pathological autoimmune conditions. Epitope mapping analysis of a mitochondrial inner membrane enzyme, citrate synthase (CS) (EC 2.3.3.1) by synthetic overlapping peptides and phage display libraries using sera from healthy individuals and from patients having systemic autoimmune disease revealed CS recognizing nAAbs with IgM isotype. We analyzed cross reactive epitopes on human CS, bacterial CS, and various standard autoantigens. The anti-CS nAAbs by participating in the nAb network, could function in innate defense mechanisms and at the same time recognize a target antigen (nucleosome) in a systemic autoimmune disease. Thus, at the level of recognized epitopes there is a possible new link between the innate like component and the adaptive-autoimmune arm of the humoral immune system.
authors.affiliation: Department of Immunology and Biotechnology, Faculty of Medicine, University of Pécs, Szigeti út 12., Pécs H-7643, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Czömpöly
authors.forename: Katalin
authors.initials: K
authors.lastname: Olasz
authors.forename: Diána
authors.initials: D
authors.lastname: Simon
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Nyárády
authors.forename: László
authors.initials: L
authors.lastname: Pálinkás
authors.forename: László
authors.initials: L
authors.lastname: Czirják
authors.forename: Tímea
authors.initials: T
authors.lastname: Berki
authors.forename: Péter
authors.initials: P
authors.lastname: Németh
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001323:Autoantibodies / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology; D015551:Autoimmunity / Q000276:immunology; D016022:Case-Control Studies; D002648:Child; D002846:Chromatography, Affinity; D002950:Citrate (si)-Synthase / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007110:Immunity, Active / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D008297:Male; D008928:Mitochondria / Q000201:enzymology* / Q000276:immunology*; D008969:Molecular Sequence Data; D019151:Peptide Library
chemical_list: D001323:Autoantibodies; D007106:Immune Sera; D019151:Peptide Library; D002950:Citrate (si)-Synthase