Purification of Poly-beta-Hydroxybutyrate by Density Gradient Centrifugation in Sodium Bromide.
Fractionation of fully sporulated cultures of Bacillus thuringiensis by density gradient centrifugation in NaBr produced two bands which were identified as poly-beta-hydroxybutyrate. This technique generated high yields of membrane-bound and unbound granules of exceptional purity and degree of polymerization.
Nickerson
K W
KW
School of Life Sciences, University of Nebraska, Lincoln, Nebraska 68588.