title: Interactions among the components of the interleukin-10 receptor complex.
abstract: We used fluorescence resonance energy transfer previously to show that the interferon-gamma (IFN-gamma) receptor complex is a preformed entity mediated by constitutive interactions between the IFN-gammaR2 and IFN-gammaR1 chains, and that this preassembled entity changes its structure after the treatment of cells with IFN-gamma. We applied this technique to determine the structure of the interleukin-10 (IL-10) receptor complex and whether it undergoes a similar conformational change after treatment of cells with IL-10. We report that, like the IFN-gamma receptor complex, the IL-10 receptor complex is preassembled: constitutive but weaker interactions occur between the IL-10R1 and IL-10R2 chains, and between two IL-10R2 chains. The IL-10 receptor complex undergoes no major conformational changes when cells are treated with cellular or Epstein-Barr viral IL-10. Receptor complex preassembly may be an inherent feature of Class 2 cytokine receptor complexes.
authors.affiliation: Department of Molecular Genetics, Microbiology, and Immunology, The University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, NJ 08854, USA.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Krause
authors.forename: Erwen
authors.initials: E
authors.lastname: Mei
authors.forename: Olga
authors.initials: O
authors.lastname: Mirochnitchenko
authors.forename: Natasha
authors.initials: N
authors.lastname: Lavnikova
authors.forename: Junxia
authors.initials: J
authors.lastname: Xie
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Jia
authors.forename: Robin M
authors.initials: RM
authors.lastname: Hochstrasser
authors.forename: Sidney
authors.initials: S
authors.lastname: Pestka
mesh_terms: D000818:Animals; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D031541:Fluorescence Resonance Energy Transfer; D016753:Interleukin-10 / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism*; D017471:Receptors, Interferon / Q000737:chemistry / Q000378:metabolism; D018123:Receptors, Interleukin / Q000737:chemistry / Q000378:metabolism*; D053701:Receptors, Interleukin-10; D000096743:Interferon gamma Receptor
chemical_list: D016753:Interleukin-10; D008565:Membrane Proteins; D021122:Protein Subunits; D017471:Receptors, Interferon; D018123:Receptors, Interleukin; D053701:Receptors, Interleukin-10; D000096743:Interferon gamma Receptor