title: l-Tryptophan Production by Achromobacter liquidum.
abstract: Conditions for the production of tryptophanase from Achromobacter liquidum and for the conversion of l-serine and indole to l-tryptophan were studied. The enzyme could be produced in amounts as great as 0.750 U/ml (degradation) and 0.294 U/ml (synthesis) by shaking cultures at 30 degrees C in a medium containing dextrin, yeast extract, l-tryptophan, and l-glutamic acid. l-Tryptophan was produced most efficiently by shaking the cells at 37 degrees C in a reaction mixture containing 60 mg of l-serine per ml, 60 mg of indole per ml, and 0.5 mM pyridoxal phosphate. After 3 days, 96 mg of l-tryptophan per ml was formed, and l-tryptophan was easily isolated to 85.4% yield by concentration of the reaction mixture.
authors.affiliation: Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Yodogawa-ku, Osaka 532, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ujimaru
authors.forename: T
authors.initials: T
authors.lastname: Kakimoto
authors.forename: I
authors.initials: I
authors.lastname: Chibata