Preparative separation of cichoric acid from Echinacea purpurea by pH-zone-refining counter-current chromatography.
pH-zone-refining counter-current chromatography was successfully applied to the separation of cichoric acid from Echinacea Purpurea (L.) Moench. A 3.0 g quantity of sample was separated using the following two-phase solvent system: MtBE-CH3CN-water (4:1:5, v/v), 10 mM trifluoroacetic acid in organic stationary phase and 10 mM ammonia in aqueous mobile phase. The obtained fractions were analyzed by high-performance liquid chromatography and negative ion electrospray ionization mass spectrometry. Double separations were performed with the same solvent system yielding 563 mg cichoric acid at 95.6% purity.
Wang
Xiao
X
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China. wangx@keylab.net
Geng
Yanling
Y
Li
Fuwei
F
Gao
Qianshan
Q
Shi
Xingang
X
D002109:Caffeic Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D020900:Echinacea / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D013386:Succinates / Q000302:isolation & purification*
D002109:Caffeic Acids; D013386:Succinates; C100435:chicoric acid