title: Exploration for unknown substances in rapeseed oil that shorten survival time of stroke-prone spontaneously hypertensive rats. Effects of super critical gas extraction fractions.
abstract: To identify the causative substances for the shortening of survival time by rapeseed (Canola) oil in stroke-prone spontaneously hypertensive rats (SHRSP), SHRSP were fed on a standard chow supplemented with 10 w/w% soybean oil (control), rapeseed oil, one of the fractions of rapeseed oil obtained by super critical gas extraction (SCE) under a pressure of 180-bar or 350-bar, at 40 degrees C, or the residue from the extraction (with 0.5% NaCl in drinking water). In another series of experiment, SHRSP were fed for 8 weeks on the above-mentioned diets without salt loading and autopsied. Fatty acid compositions in these diets were similar, except in the soybean oil diet, and phytosterol contents were: (diet containing) 180-bar fraction>residue>rapeseed oil>350-bar fraction>soybean oil. Survival times in the rapeseed oil, 350-bar fraction and residue groups were shorter than, whereas that in the 180-bar fraction was similar to in the soybean oil group. In the 8-week feeding experiment, chronic nephropathy was found frequently in the groups other than the soybean oil group. The heart weights were higher in the rapeseed oil and residue groups. Cerebral necrosis was found in the residue group. Taken together, the followings are concluded, (1) Neither the fatty acid composition, nor the amount of phytosterols in the diets appeared to be decisive in the shortening of life. (2) SCE appeared to produce a safe (180-bar) fraction, though it failed to separate clearly the causative substances into specific fractions. (3) The factors that facilitate the genetic disease of SHRSP appear to exist in rapeseed oil. However, they might not be identical to those responsible for the life-shortening, since there were no findings common across the rapeseed oil, 350-bar and residue groups, which showed similar life-shortening.
authors.affiliation: Hatano Research Institute, Food and Drug Safety Center, 729-5 Ochiai, Hadano, Kanagawa 257-8523, Japan. ohara.n@fdsc.or.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Ohara
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Naito
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nagata
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Tatematsu
authors.forename: Shin-Ya
authors.initials: SY
authors.lastname: Fuma
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Tachibana
authors.forename: Harumi
authors.initials: H
authors.lastname: Okuyama
mesh_terms: D000465:Algorithms; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004326:Drinking; D004435:Eating; D005227:Fatty Acids / Q000032:analysis; D005229:Fatty Acids, Monounsaturated; D007677:Kidney Function Tests; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010840:Phytosterols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity; D010938:Plant Oils / Q000737:chemistry* / Q000633:toxicity*; D000074262:Rapeseed Oil; D051381:Rats; D011918:Rats, Inbred SHR; D013024:Soybean Oil / Q000737:chemistry / Q000633:toxicity; D016019:Survival Analysis; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D010840:Phytosterols; D010936:Plant Extracts; D010938:Plant Oils; D000074262:Rapeseed Oil; D013024:Soybean Oil