title: Myricetin stimulates the absorption of the pro-carcinogen PhIP.
abstract: The effect of the flavonoid myricetin on the transport of the pro-carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) through differentiated Caco-2 monolayers, a model for the intestinal epithelium, is described. Myricetin causes an increase of the transport of PhIP from the apical to the basolateral compartment. This effect was observed at physiologically relevant concentrations of PhIP and myricetin. Cyclosporin A (MRP2 inhibitor) but not PSC833 (P-gp inhibitor) showed a similar effect on PhIP transport. The results indicate that myricetin induces an increased basolateral uptake of the pro-carcinogen PhIP, in part through inhibition of the MRP2 mediated excretion of PhIP from the intestinal cells back to the lumen.
authors.affiliation: Division of Toxicology, Wageningen University, P.O. Box 8000, 6700 EA Wageningen, The Netherlands. Maaike.schutte@wur.nl
authors.forename: Maaike E
authors.initials: ME
authors.lastname: Schutte
authors.forename: Johannes J M
authors.initials: JJ
authors.lastname: van de Sandt
authors.forename: Gerrit M
authors.initials: GM
authors.lastname: Alink
authors.forename: John P
authors.initials: JP
authors.lastname: Groten
authors.forename: Ivonne M C M
authors.initials: IM
authors.lastname: Rietjens
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000502:physiology; D000042:Absorption; D018938:Caco-2 Cells; D002273:Carcinogens / Q000493:pharmacokinetics*; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D007093:Imidazoles / Q000493:pharmacokinetics*; D026901:Membrane Transport Proteins / Q000378:metabolism; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D010539:Permeability
chemical_list: D018528:ATP-Binding Cassette Transporters; D002273:Carcinogens; D005419:Flavonoids; D007093:Imidazoles; D026901:Membrane Transport Proteins; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C000715087:ABCC2 protein, human; C040015:myricetin; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine