Growth Kinetics of Thiobacillus ferrooxidans Isolated from Arsenic Mine Drainage.
Thiobacillus ferrooxidans is found in many Alaskan and Canadian drainages contaminated by metals dissolved from placer and lode gold mines. We have examined the iron-limited growth and iron oxidation kinetics of a T. ferrooxidans isolate, AK1, by using batch and continuous cultures. Strain AK1 is an arsenic-tolerant isolate obtained from placer gold mine drainage containing large amounts of dissolved arsenic. The steady-state growth kinetics are described with equations modified for threshold ferrous iron concentrations. The maximal specific growth rate (mu(max)) for isolate AK1 at 22.5 degrees C was 0.070 h, and the ferrous iron concentration at which the half-maximal growth rate occurred (K(mu)) was 0.78 mM. Cell yields varied inversely with growth rate. The iron oxidation kinetics of this organism were dependent on biomass. We found no evidence of ferric inhibition of ferrous iron oxidation for ferrous iron concentrations between 9.0 and 23.3 mM. A supplement to the ferrous medium of 2.67 mM sodium arsenite did not result in an increased steady-state biomass, nor did it appear to affect the steady-state growth kinetics observed in continuous cultures.
Braddock
J F
JF
Institute of Water Resources/Engineering Experiment Station, University of Alaska, Fairbanks, Alaska 99701.
Luong
H V
HV
Brown
E J
EJ