[Analysis of sucrose esters by thin-layer chromatography].
The thin-layer chromatographic method of sucrose esters was developed by the use of toluene-ethyl acetate-methanol-water (10:5:4.5:0.2 in volume ratio) as a mobile phase. Sucrose esters were developed by ascending technique on the silica gel G plate, visualized by urea-phosphoric acid-n-butanol with blue spots. The Rf value of monoester was 0.16 and those of higher esters were 0.38 - 0.93. The calibration curve was linear in the range of 25 microg - 250 microg with the detection limit of 0.1 microg. Accuracies were certified by normalization method and external standard method with t-test. The results were /t/ = 0.627(< 2.571) and /t/ = 1.123(<2.571) and RSDs were 3.03% and 3.08% (n = 6) respectively for S-1570. Contents of monoester of different sucrose esters were determined, which could provide reference for synthetic conditions and application range of sucrose esters.
Li
Yan-Ke
YK
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China.
Zhang
Shu-Fen
SF
Yang
Jin-Zong
JZ
D002855:Chromatography, Thin Layer / Q000379:methods*; D004952:Esters / Q000032:analysis*; D057230:Limit of Detection; D013395:Sucrose / Q000032:analysis*
D004952:Esters; D013395:Sucrose