title: [Cloning, expression and characterization of antibacterial peptide CM4 in Pichia pastoris].
abstract: To explore a new approach of expression of ABP-CM4 in methylotrophic yeast P. pastoris. The gene of ABP-CM4 was cloned into the vector pPIC9. The Sac I -linearized plasmid pPIC9-CM4 was transformed into P. pastoris GS115 by electroporation. By means of MM and MD plates and PCR, the recombinant P. pastoris strains (his- mut+) were obtained. Antibacterial assay, Tricine-SDS-PAGE and Polyarylamide Gel Electrophoresis demonstrated that ABP-CM4 was extracellularly expressed in P. pastoris with the induction of methanol. The recombinant ABP-CM4 has the antifungal activity on Aspergillus niger and Trichoderma viride.
authors.affiliation: Jiangsu Province Key Laboratory of Biochemistry and Molecular Biology, College of Life Science, Nanjing Normal University, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Wu
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Yue
authors.forename: Yu-qing
authors.initials: YQ
authors.lastname: Chen
authors.forename: Shuang-quan
authors.initials: SQ
authors.lastname: Zhang
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000494:pharmacology; D001234:Aspergillus niger / Q000187:drug effects; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology
chemical_list: D023181:Antimicrobial Cationic Peptides; C534224:CM4 peptide, Bombyx mori; D011994:Recombinant Proteins