CTX-M-1 extended-spectrum beta-lactamase-producing Proteus mirabilis in Greece.
To examine the dissemination of CTX-M-type extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Proteus mirabilis, 91 nonrepetitive ESBL-producing P. mirabilis were collected from infected patients in a tertiary Greek hospital during September, 2001, to May, 2004. A bla (CTX-M) gene was amplified in one isolate (strain A328), but bla (CTX-M) was not detected in any of the remaining ESBL producers. Sequencing results showed that P. mirabilis A328 produced a CTX-M-1 enzyme while PCR mapping of the genetic element carrying bla (CTX-M-1) revealed that the gene was located downstream of an ISEcp1B element. The cefotaxime resistance determinant was easily transferable and carried on a 70-kb plasmid. The emergence of CTX-M-1- producing P. mirabilis indicates the need for early recognition of such strains to be able to control their spread in our hospital and community environment.
Karapavlidou
Paraskevi
P
Department of Microbiology, Hippokration University Hospital, 546 42 Thessaloniki, Greece.
Sofianou
Danai
D
Manolis
Evangelos N
EN
Pournaras
Spyros
S
Tsakris
Athanassios
A
D006115:Greece; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011513:Proteus mirabilis / Q000201:enzymology* / Q000302:isolation & purification; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
C055184:beta-lactamase TEM-3; D001618:beta-Lactamases