Simultaneous densitometric determination of artemisinin, artemisinic acid and arteannuin-B in Artemisia annua using reversed-phase thin layer chromatography.
A rapid and simple RP-TLC method for simultaneous quantification of pharmacologically important sesquiterpene artemisinin (AM) together with its precursors arteannuin-B (AB) and artemisinic acid (AA) in the inflorescence part of Artemisia annua plant has been developed. The RP-TLC of sesquiterpenes was performed on RP-18 F254 S thin-layer chromatographic plates by developing in mobile phase, containing 0.2% TFA in water/ACN (35:65, v/v). The densitometric determination of AM, AB and AA was carried out after derivatization with anisaldehyde reagent at 426 nm in absorption-reflectance mode.
Bhandari
Pamita
P
Natural Plant Products Division, Institute of Himalayan Bioresource Technology, Palampur, H P, India.
Gupta
Ajai P
AP
Singh
Bikram
B
Kaul
Vijay K
VK
D000962:Antimalarials / Q000032:analysis* / Q000302:isolation & purification; D031172:Artemisia annua / Q000737:chemistry*; D037621:Artemisinins / Q000032:analysis* / Q000302:isolation & purification; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry; D015394:Molecular Structure; D028321:Plant Preparations / Q000737:chemistry; D012717:Sesquiterpenes / Q000032:analysis* / Q000302:isolation & purification
D000962:Antimalarials; D037621:Artemisinins; D028321:Plant Preparations; D012717:Sesquiterpenes; C006634:arteannuin B; C047721:artemisic acid; C031327:artemisinin