A clinoptilolite effect on cell media and the consequent effects on tumor cells in vitro.
Clinoptilolite is a nontoxic natural zeolite with properties of an ion-exchanger and adsorbent. Earlier studies showed that clinoptilolite could be an adjuvant in cancer therapy. The aim of this study was to define effects of clinoptilolite in cell media on cell viability and activity of key proteins regulating cell survival, cell division and stress response. The number of viable cells, DNA synthesis and activity of EGF-R, PKB/Akt and NF?B was reduced, while apoptosis was increased in cells that were cultured in medium supplemneted with clinoptilolite. These results might be due to adsorbtion of some serum components such as EGF to clinoptilolite. In treated medium without serum the predominant role of clinoptilolite is that of cation exchange, likely affecting calcium levels and calcium-dependent signalling pathways. These results are in line with other data that confirm enhanced apoptosis in cells incubated in treated medium. Together, data presented here demonstrate that clinoptilolite affects cellular microenvironment through mechanisms that are dependent on adsorptive and ion-exchange characteristics of this material.
Katic
Masa
M
Division of Molecular Medicine, Rudjer Boskovic Institute, Bijenicka 54, HR-10000 Zagreb, Croatia. masa.katic@joslin.harvard.edu
Bosnjak
Berislav
B
Gall-Troselj
Koraljka
K
Dikic
Ivan
I
Pavelic
Kresimir
K
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival; D002852:Chromatography, Ion Exchange / Q000379:methods; D003470:Culture Media / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D066298:In Vitro Techniques; D007477:Ions; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013936:Thymidine / Q000737:chemistry; D013997:Time Factors; D017641:Zeolites / Q000494:pharmacology*
D003470:Culture Media; D007477:Ions; D016328:NF-kappa B; D013778:Tetrazolium Salts; D013844:Thiazoles; C083175:clinoptilolite; D017641:Zeolites; D004247:DNA; D066246:ErbB Receptors; D051057:Proto-Oncogene Proteins c-akt; D048670:MAP Kinase Kinase 4; C022616:thiazolyl blue; D002118:Calcium; D013936:Thymidine