Preparation of a Cell-Free, Isobutene-Forming System from Rhodotorula minuta.
The cell-free isobutene-forming system of Rhodotorula minuta IFO 1102 was prepared. This system consisted of isovaleric acid, NADPH, and a cell extract. The maximal activity obtained by using the described cell-free system was 17 nl/mg of protein per h. It appears that this cell-free isobutene-forming system operates in living cells of R. minuta.
Fujii
T
T
Department of Applied Microbial Technology, The Kumamoto Institute of Technology, 4-22-1 Ikeda, Kumamoto 860, Japan.
Ogawa
T
T
Fukuda
H
H