title: Protective effect of Phyllanthus fraternus against allyl alcohol-induced oxidative stress in liver mitochondria.
abstract: The effect of administration of allyl alcohol on the oxidative stress and the protective effect due to administration of an aqueous extract of Phyllanthus fraternus against allyl alcohol-induced damage in liver mitochondria were studied. When rats were treated with allyl alcohol, the rate of mitochondrial respiration was decreased significantly with both NAD(+)- and FAD-linked substrates. The respiratory control ratio, an index of membrane integrity and the P/O ratio, a measure of phosphorylation efficiency also decreased significantly. There was a significant increase in the lipid peroxide level and the protein carbonyl content. A significant decrease was observed in the total sulphydryl groups and a significant increase in the generation of superoxide radicals. Administration of rats with an aqueous extract of Phyllanthus fraternus (100 mg/kg) prior to allyl alcohol administration showed protection of 72, 40 and 80% using glutamate+malate (NADH oxidation) and 77, 54 and 20% using succinate as substrate on state 3, RCR and P/O ratio, respectively. The protection on lipid peroxide level was 88 and 91% in homogenate and mitochondria, respectively. In case of protein carbonyls, total sulphydryl groups and on the generation of superoxide radicals the protection was 99, 59 and 53%, respectively.
authors.affiliation: Department of Biochemistry, School of Life Sciences, University of Hyderabad, Gachi Bowli, Hyderabad 500046, India.
authors.forename: R
authors.initials: R
authors.lastname: Sailaja
authors.forename: O H
authors.initials: OH
authors.lastname: Setty
mesh_terms: D000818:Animals; D015227:Lipid Peroxidation; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D020005:Propanols / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D020005:Propanols; D017382:Reactive Oxygen Species; D013481:Superoxides; C006463:allyl alcohol