Differential beta-adrenoceptor expression induced by nerve growth factor infusion into the canine right and left stellate ganglia.
BACKGROUND
Nerve growth factor (NGF) infusion into the right stellate ganglion (RSG) is antiarrhythmic, while NGF infusion into the left stellate ganglion (LSG) is proarrhythmic in dogs with myocardial infarction (MI) and complete atrioventricular block (CAVB). This functional asymmetry suggests differential neural remodeling.


OBJECTIVE
To test the hypothesis that NGF infusion into the RSG and the LSG can lead to differential beta-adrenoceptor (beta-AR) expression in dogs with MI and CAVB.


RESULTS
We performed immunostaining to quantify beta(1)-AR and beta(3)-AR immunoreactivity in six dogs with MI and CAVB, nine dogs with MI, CAVB, and NGF infusion to the LSG, six dogs with MI, CAVB, and NGF infusion to the RSG, and six normal dogs. There was significantly increased beta(3)-AR immunoreactivity in dogs with NGF infusion into the LSG and significantly decreased beta(3)-AR immunoreactivity in dogs with NGF infusion into the RSG compared with controls and with the MI and CAVB group. There were no significant differences in beta(1)-AR immunoreactivity among these four groups. To determine protein and mRNA expression of beta-ARs, we created MI and CAVB and infused NGF into the LSG in six additional dogs. The noninfarcted left ventricle free wall was harvested 1 week later. The protein level and receptor density of beta(3)-AR (but not beta(1)- or beta(2)-AR) significantly increased in these six dogs compared with normal controls.


CONCLUSIONS
We conclude that NGF infusion into the RSG and the LSG in dogs with MI and CAVB induced differential beta(3)-AR expression in the left ventricular myocardium.
Zhou
Shengmei
S
Division of Cardiology, Department of Medicine, University of Southern California, USA.
Paz
Offir
O
Cao
Ji-Min
JM
Asotra
Kamlesh
K
Chai
Ning-Ning
NN
Wang
Charles
C
Chen
Lan S
LS
Fishbein
Michael C
MC
Sharifi
Behrooz
B
Chen
Peng-Sheng
PS
D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D006327:Heart Block / Q000150:complications / Q000378:metabolism; D006352:Heart Ventricles / Q000378:metabolism; D015918:Infusion Pumps, Implantable; D009203:Myocardial Infarction / Q000150:complications / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D020932:Nerve Growth Factor / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D018342:Receptors, Adrenergic, beta-1 / Q000378:metabolism*; D022702:Receptors, Adrenergic, beta-3 / Q000378:metabolism*; D013233:Stellate Ganglion / Q000187:drug effects*; D017180:Tachycardia, Ventricular / Q000209:etiology; D015854:Up-Regulation
D012333:RNA, Messenger; D018342:Receptors, Adrenergic, beta-1; D022702:Receptors, Adrenergic, beta-3; D020932:Nerve Growth Factor