[Therapeutic effect of Seaweed Polysaccharide from Sargassum confusum on sarcoma s180 in mice and its mechanism].
OBJECTIVE
Previous chemotherapeutic drugs have side effects which restrict their application in treating tumors. This research was to explore the inhibitory effect of wild Seaweed Polysaccharide from Sargassum confusum (SP) on sarcoma S180 xenograft in mice and its mechanism to offer experimental basis for searching for antitumor drugs with high efficiency and low toxicity.


METHODS
SP were prepared and injected abdominally in mice at the doses of 50, 100, and 200 mg/kg, respectively, for 10 days before tumor cell transplantation. The mice with injection of 0.85% normal saline were set as negative control. The weight of S180 xenografts in mice was measured to calculate the inhibitory rate of SP on S180 tumor; the cell cycle of thymocytes and splenocytes were measured by flow cytometry (FCM); DNA and protein syntheses in thymocytes and splenocytes were measured by incorporation of (3)H-TdR; the immunopotentiative effect of SP on mice was assessed by splenic lymphocytes transformation assay.


RESULTS
The inhibitory rates of S180 xenografts in mice treated with 50, 100, and 200 mg/kg SP were 28.7%, 32.9%, and 50.3%, respectively. The thymocytes and splenocytes functions were significantly enhanced in SP groups than in control group (P<0.05, P<0.01).


CONCLUSIONS
SP might inhibit the growth of S180 xenograft in mice through improving the functions of thymocytes and splenocytes.
Liu
Qiu-Ying
QY
Guangdong Prevention and Treatment Center for Occupational Diseases, Guangzhou, Guangdong 510300, P. R. China.
Meng
Qing-Yong
QY
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D011134:Polysaccharides / Q000302:isolation & purification / Q000627:therapeutic use*; D011897:Random Allocation; D012510:Sarcoma 180 / Q000188:drug therapy / Q000473:pathology*; D044647:Sargassum / Q000737:chemistry; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
D011134:Polysaccharides; D004247:DNA