title: Abomasal bacteria produce an inhibitor of gastrin secretion in vitro.
abstract: Previously, proliferating microflora transferred with abomasal nematodes, were suspected to be the source of the gastrin inhibitor in some parasite excretory/secretory products. Aerobic cultures in HBSS of abomasal fluid from uninfected sheep became inhibitory during the static growth phase, unless antibiotics were present. Basal gastrin secretion was reduced by up to 90%. Rumen fluid and incubates and medium in which Streptococcus bovis and ovine rumen Actinomycete spp. had been grown also contained the inhibitor. Unlike abomasal cultures, rumen fluid and incubates also reduced the measurement of gastrin standards. Rumen incubates were less potent after exposure to pH 2-3, suggesting that inactivation normally occurs in the unparasitised abomasum. Contaminating bacteria which generate the gastrin inhibitor in parasite ES products are probably rumen organisms which survive in the abomasum and proliferate during subsequent incubation. Significantly, rumen bacteria have been shown to be capable of affecting the secretory activity of the gastric mucosa.
authors.affiliation: Institute of Food, Nutrition and Human Health, Massey University, Private Bag 11-222, Palmerston North, New Zealand.
authors.forename: D C
authors.initials: DC
authors.lastname: Simcock
authors.forename: D E B
authors.initials: DE
authors.lastname: Lawton
authors.forename: I
authors.initials: I
authors.lastname: Scott
authors.forename: H V
authors.initials: HV
authors.lastname: Simpson
mesh_terms: D000018:Abomasum / Q000382:microbiology* / Q000469:parasitology; D039903:Actinobacteria / Q000302:isolation & purification / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D005753:Gastric Mucosa / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism*; D009349:Nematode Infections / Q000150:complications; D012756:Sheep
chemical_list: D003470:Culture Media; D005755:Gastrins