title: Fluorimetric Detection of a Bacillus stearothermophilus Spore-Bound Enzyme, alpha-d-Glucosidase, for Rapid Indication of Flash Sterilization Failure.
abstract: A biological indicator based on fluorimetric detection within 60 min of a Bacillus stearothermophilus spore-bound enzyme, alpha-d-glucosidase, has been developed. Results indicate that the enzyme survived slightly longer than spores observed after 24 h of incubation. The new system shows promise for evaluating flash sterilization cycles within 60 min compared with conventional 24-h systems.
authors.affiliation: School of Public Health, Box 197 Mayo, University of Minnesota, Minneapolis, Minnesota 55455, and 3M Company, St. Paul, Minnesota 55133.
authors.forename: D
authors.initials: D
authors.lastname: Vesley
authors.forename: A C
authors.initials: AC
authors.lastname: Langholz
authors.forename: S R
authors.initials: SR
authors.lastname: Rohlfing
authors.forename: W E
authors.initials: WE
authors.lastname: Foltz