title: Selection of peptides for lipopolysaccharide binding on to epoxy beads and selective detection of Gram-negative bacteria.
abstract: Lipopolysaccharide (LPS)-binding peptides were enriched by using epoxy beads as a novel support to immobilize LPS for a phage displayed peptide library screening. The sequence of Phe-Ala-Pro-Trp (FAPW) was the most significant consensus motif of 10 selected clones, and Pro-Phe (PF) was the key dipeptide for binding at the apex of the loop to form a characteristic structure of CXXPFXXXC. Moreover, AWLPWAK, one of the highly conserved heptamer peptides, could detect specifically Gram-negative bacteria via a whole cell binding test at 10(6) cells ml(-1).
authors.affiliation: Program in Biochemical Engineering and Biotechnology, Seoul National University, Gwanak-Gu, Korea.
authors.forename: Yun-Gon
authors.initials: YG
authors.lastname: Kim
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Woo-Jae
authors.initials: WJ
authors.lastname: Chung
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Kim
authors.forename: Dong-Sik
authors.initials: DS
authors.lastname: Shin
authors.forename: June-Hyung
authors.initials: JH
authors.lastname: Kim
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
authors.forename: Junho
authors.initials: J
authors.lastname: Chung
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
mesh_terms: D015373:Bacterial Typing Techniques; D004852:Epoxy Compounds / Q000737:chemistry*; D006090:Gram-Negative Bacteria; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D008863:Microspheres; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012680:Sensitivity and Specificity
chemical_list: D004852:Epoxy Compounds; D008070:Lipopolysaccharides; D009842:Oligopeptides