Transposon mutagenesis in rhizobia which can nodulate both legumes and the nonlegume parasponia.
The successful use of transposon mutagenesis in a slow-growing Rhizobium permitted the isolation of auxotrophic and symbiotically defective mutants. In these mutants, it was shown that different single-copy Tn5 insertions had occurred. Mutant phenotypes indicated that the control of nitrogenase activity in vitro and in planta is different.
Cen
Y
Y
Genetics Department, Research School of Biological Sciences, and Botany Department, Australian National University, Canberra City, A.C.T. 2601, and Division of Land Resources Management, CSIRO, Wembley, Western Australia, 6014, Australia.
Bender
G L
GL
Trinick
M J
MJ
Morrison
N A
NA
Scott
K F
KF
Gresshoff
P M
PM
Shine
J
J
Rolfe
B G
BG