Improved double-vial radiorespirometric technique for mineralization of C-labeled substrates.
New procedures which simplify sample preparation and improve counting efficiency were developed for double-vial radiorespirometry. Under certain conditions, efficiency of fluor-NaOH-impregnated wicks was not stable, but was adversely affected by water vapor, NaOH concentration, and CO(2) loading. Glass fiber wick material treated with a methanolic fluor-NaOH solution showed improved C counting efficiency (58%) compared with previous methods.
Coveney
M F
MF
W. K. Kellogg Biological Station, Michigan State University, Hickory Corners, Michigan 49060.
Wetzel
R G
RG