Inhibitory effects of alpha-zearalenol on angiotensin II-induced integrin beta3 mRNA via suppression of nuclear factor-kappaB.
OBJECTIVE
To investigate the effect of alpha-zearalenol on angiotensin II-induced beta3 integrin mRNA expression in human umbilical vein endothelial cells (HUVECs).


METHODS
The mRNA level in integrin beta3 was determined by reverse transcription-polymerase chain reaction. Endothelial NF-kappaB activity was determined by the luciferase activity assay of plasmid NF-kappaB-LUC.


RESULTS
The angiotensin II-induced beta3 integrin mRNA expression was inhibited by alpha-zearalenol and 17beta-estradiol (10 nmol/L -1 micromol/L), but not influenced by ICI 182, 780, a pure competitive antagonist for estrogen receptor or a nitric oxide inhibitor Nomega-Nitro-L-arginine methyl ester hydrochloride. Alpha-zearalenol and 17beta-estradiol suppressed the angiotensin II-induced activation of NF-kappaB in endothelial cells.


CONCLUSIONS
Alpha-zearalenol inhibits angiotensin II-induced integrin beta3 mRNA expression by suppressing NF-kappaB activation in endothelial cells.
Li
Su-Min
SM
Department of Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
Wang
Xiao-Ming
XM
Qiu
Jin
J
Si
Qin
Q
Guo
Heng-Yi
HY
Sun
Ren-Yu
RY
Wu
Qi-Xia
QX
D000804:Angiotensin II / Q000037:antagonists & inhibitors; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D039661:Integrin beta3 / Q000096:biosynthesis* / Q000235:genetics; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000502:physiology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D048789:Phytoestrogens / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000037:antagonists & inhibitors; D015029:Zeranol / Q000031:analogs & derivatives* / Q000494:pharmacology
D039661:Integrin beta3; D016328:NF-kappa B; D048789:Phytoestrogens; D012333:RNA, Messenger; D011960:Receptors, Estrogen; C029659:zearalenol; D000804:Angiotensin II; D009569:Nitric Oxide; D004958:Estradiol; D015029:Zeranol