Immobilization of cryopreserved primary rat hepatocytes for the development of a bioartificial liver system.
Primary rat hepatocytes were cryopreserved in hormonally-defined medium (HDM) containing 40% (v/v) fetal bovine serum (FBS) and 10% (v/v) dimethyl sulfoxide (DMSO) in liquid N2 for 6 months. After thawing, the cells were immobilized using 2% (w/v) alginate and 0.5% (w/v) chitosan solutions. The capacities of ammonia removal and urea synthesis of the immobilized-thawed hepatocytes were similar to those of immobilized hepatocytes without cryopreservation. This result shows that immobilized hepatocytes after cryopreservation are useful for the development of a bioartificial liver system.
Son
Jeong Hwa
JH
Department of Biotechnology and Bioengineering, Pukyong National University, 608-737, Busan, Korea.
Ha
Yu-Mi
YM
Kim
Yun-Im
YI
Kim
Ki Myong
KM
Park
Jung-Keug
JK
Kim
Sung-Koo
SK
D000464:Alginates / Q000737:chemistry; D000818:Animals; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques; D018914:Cells, Immobilized; D048271:Chitosan / Q000737:chemistry; D015925:Cryopreservation; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D019164:Liver, Artificial; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012996:Solutions / Q000737:chemistry*; D013997:Time Factors
D000464:Alginates; D012996:Solutions; D048271:Chitosan