Cytochemical Localization of Urease in a Rumen Staphylococcus sp. by Electron Microscopy.
We describe a technique whereby intracellular urease activity can be localized by transmission electron microscopy. The ammonia produced from the enzymatic hydrolysis of urea is first precipitated with sodium tetraphenylboron and then replaced with silver to produce electron-dense silver tetraphenylboron. This direct reaction product deposition procedure was used to demonstrate the presence of membrane-bound urease of Staphylococcus sp. H3-22, a gram-positive ruminal bacterium.
McLean
R J
RJ
Department of Biology, University of Calgary, Calgary, Alberta, Canada T2N 1N4; Agriculture Canada Research Station, Lethbridge, Alberta, Canada T1J 4B1; and Allied Chemicals Ltd., Syracuse Research Laboratory, Solvay, New York 13209.
Cheng
K J
KJ
Gould
W D
WD
Costerton
J W
JW