LC/MS fingerprinting of Shenmai injection: a novel approach to quality control of herbal medicines.
Chromatographic fingerprinting has been recommended as a potential and reliable strategy for the quality control of herbal medicines. Although varieties of chromatographic techniques, particularly HPLC, have been widely employed, hyphenated chromatographic approach has not been sufficiently exploited in chromatographic fingerprinting. In this work, LC/MS fingerprinting of Shenmai injection was developed. Thirty ginsenosides as well as seven ophioponins were selected to construct the LC/MS fingerprint using selective ion monitoring (SIM) mode, while previous HPLC fingerprint [H.J. Zhang, Y.J. Wu, Y.Y. Cheng, J. Pharm. Biomed. Anal. 31 (2003) 175-183] only represents the ginsenosides. Subsequently, the proposed LC/MS fingerprints were applied to identifying the product manufacturers. All the samples were accurately classified based on their LC/MS fingerprints in conjunction with principal components analysis (PCA). This study would be potentially helpful to improve the quality control ability of fingerprinting-based strategy for complex herbal medicines.
Xiaohui
Fan
F
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027, PR China.
Yi
Wang
W
Yiyu
Cheng
C
D002491:Central Nervous System Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D036145:Ginsenosides / Q000032:analysis; D007202:Indicators and Reagents; D013058:Mass Spectrometry / Q000379:methods*; D019999:Pharmaceutical Solutions / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results
D002491:Central Nervous System Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D007202:Indicators and Reagents; D019999:Pharmaceutical Solutions; D010936:Plant Extracts; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination