Separation of methanotrophic bacteria by using percoll and its application to isolation of mixed and pure cultures.
Type I and II methanotrophic bacteria can be cleanly separated by using Percoll buoyant density gradients. This simple and inexpensive method can be used to screen new isolates rapidly for preliminary classification. In addition, Percoll gradients can also be used to enhance standard enrichment techniques for methanotrophs from natural water samples.
Putzer
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Center for Great Lakes Studies and Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53201, and Keck Laboratories, 138-78 California Institute of Technology, Pasadena, California 91125.
Buchholz
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LA
Lidstrom
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ME
Remsen
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