Static adsorptive coating of poly(methyl methacrylate) microfluidic chips for extended usage in DNA separations.
A simple and robust static adsorptive (dynamic) coating process using 2% hydroxyethylcellulose was developed for surface modification of poly(methyl methacrylate) (PMMA) microfluidic chips for DNA separations, suitable for usage over extended periods, involving hundreds of runs. The coating medium was also used as a sieving matrix for the DNA separations following the coating process. Four consecutive static treatments, by simply filling the PMMA chip channels with sieving matrix once every day, were required for obtaining a stable coating and optimum performance. The performance of the coated chips at different phases of the coating process was studied by consecutive gel electrophoretic separations with LIF detection using a PhiX-174/HaeIII DNA digest sample. The coated chip, with daily renewal of the sieving matrix, showed high stability in performance during a 25-day period of systematic study, involving more than 100 individual runs. The performance of the coated chip also remained almost the same after 3 months of continuous usage, during which over 200 separations were performed. The average precision of migration time for the 603-bp fragment was 1.31% RSD (n = 6) during the 25-day study, with a separation efficiency of 6.5 x 10(4) plates (effective separation length 5.4 cm).
Du
Xiao-Guang
XG
Research Center for Analytical Sciences, Northeastern University, Shenyang, China.
Fang
Zhao-Lun
ZL
D000327:Adsorption; D010584:Bacteriophage phi X 174 / Q000235:genetics; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004247:DNA / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D019904:Polymethyl Methacrylate / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D004279:DNA, Viral; D004247:DNA; D019904:Polymethyl Methacrylate; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases