title: Evaluation of a rapid PCR-based method for the detection of animal material.
abstract: A rapid PCR-based analytical method for detection of animal-derived materials in complete feed was developed. Using a commercially available DNA forensic kit for the extraction of DNA from animal feed, a sensitive method was developed that was capable of detecting as little as 0.03% bovine meat and bone meal in complete feed in under 8 h of total assay time. The reduction in assay time was accomplished by reducing the DNA extraction time to 2 h and using the simpler cleanup procedure of the kit. Assay sensitivity can be increased to 0.006% by increasing the DNA extraction time to an overnight incubation of approximately 16 h. Examination of dairy feed samples containing either bovine meat and bone meal, porcine meat and bone meal, or lamb meal at a level of 0.1% (wt/wt basis) suggested that this method may be suitable for regulatory uses. The adoption of this commercially available kit for use with animal feeds yields an assay that is quicker and simpler to perform than a previously validated assay for the detection of animal proteins in animal feed.
authors.affiliation: Division of Animal Research, U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, Maryland 20708, USA.
authors.forename: Haile F
authors.initials: HF
authors.lastname: Yancy
authors.forename: Anuja
authors.initials: A
authors.lastname: Mohla
authors.forename: Dorothy E
authors.initials: DE
authors.lastname: Farrell
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Myers
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D016643:Encephalopathy, Bovine Spongiform / Q000517:prevention & control / Q000635:transmission; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013997:Time Factors
chemical_list: D004247:DNA