[Genetic analysis and mapping of the rice leafy-hull mutant Oslh].
Oslh (lh=leafy hull), in the japonica cultivar 9522 background, a mutant of Oryza sativa L. spp. japonica cv. 9522 identified from an M(2) population, was mutagenized by irradiation with (60)Co gamma-ray. The Oslh mutant plants flowered about 15 days later than the wild-type plants (Fig.1e). The paleas, lemmas and lodicules of the flowers of Oslh mutant were transformed into leaf-like structures (Fig.1b, d). Genetic analysis of the F(2) progeny from a cross between the Oslh mutant and wild-type japonica cv. 9522 revealed that the Oslh mutant arouse from a single recessive nuclear gene mutation of the cv. 9522. To map the Oslh locus, an F(2) population generated by crossing between Oslh (japonica) mutant and Guangluai4 (indica) was analyzed. The Oslh locus was mapped to the long arm of rice chromosome 3, between a SSR marker RM5475 and an InDel marker GY305, 2.9 and 1.5 cM away from these two markers respectively (Fig.4). These results are useful for further cloning and functional analysis of the OsLH gene.
Chu
Huang-Wei
HW
College of Life Science and Technology, Shanghai Jiaotong University, Shanghai 200240, China.
Liu
Hai-Sheng
HS
Li
Hui
H
Wang
Hong-Mei
HM
Wei
Jia-Li
JL
Li
Na
N
Ding
Shu-Yan
SY
Huang
Hai
H
Ma
Hong
H
Huang
Chao-Feng
CF
Luo
Da
D
Yuan
Zhen
Z
Liu
Wen-Xuan
WX
Zhang
Da-Bing
DB
D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D005720:Gamma Rays; D017343:Genes, Plant / Q000235:genetics*; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D013997:Time Factors