title: Isolation and Identification of Vesicular-Arbuscular Mycorrhiza-Stimulatory Compounds from Clover (Trifolium repens) Roots.
abstract: Two isoflavonoids isolated from clover roots grown under phosphate stress were characterized as formononetin (7-hydroxy,4'-methoxy isoflavone) and biochanin A (5,7-dihydroxy,4'-methoxy isoflavone). At 5 ppm, these compounds stimulated hyphal growth in vitro and root colonization of an undescribed vesicular-arbuscular mycorrhiza, a Glomus sp. (INVAM-112). The permethylated products of the two compounds were inactive. These findings suggest that the isoflavonoids studied may act as signal molecules in vesicular-arbuscular mycorrhiza symbiosis.
authors.affiliation: Bioactive Natural Products Laboratory, Department of Horticulture and Pesticide Research Center, and Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824.
authors.forename: M G
authors.initials: MG
authors.lastname: Nair
authors.forename: G R
authors.initials: GR
authors.lastname: Safir
authors.forename: J O
authors.initials: JO
authors.lastname: Siqueira