Tandem mass spectrometry of coprogen and deferoxamine hydroxamic siderophores.
Mechanisms of fragmentation of hydroxamic siderophores are proposed comparing deuterated and nondeuterated samples. Standard siderophores (e.g. deferoxamine and coprogen) were directly injected into both ion trap and linear quadrupole mass spectrometers with electrospray ionization (ESI). Four and two fragmentation steps were carried out for deferoxamine and coprogen (analyzed by positive and negative ESI, respectively). Deferoxamine cleavages occurred in both peptide and hydroxamic bonds while the coprogen fragmentation pattern is more elaborate, since it contains Fe(III) in its structure.
Simionato
Ana V C
AV
Laboratório de Cromatografia, Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador Sãocarlense, 400 São Carlos, SP 13566-590, Brasil.
de Souza
Gezimar D
GD
Rodrigues-Filho
Edson
E
Glick
James
J
Vouros
Paul
P
Carrilho
Emanuel
E
D002853:Chromatography, Liquid / Q000379:methods*; D003676:Deferoxamine / Q000032:analysis*; D006877:Hydroxamic Acids / Q000032:analysis*; D017262:Siderophores / Q000032:analysis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D006877:Hydroxamic Acids; D017262:Siderophores; C010896:coprogen; D003676:Deferoxamine