Ultrastructural organization of Alicyclobacillus tolerans strain K1T cells.
Electron microscopy examinations of thin sections and freeze-fracture replicas revealed the specific ultrastructural features of Alicyclobacillus tolerans strain K1(T). In particular, the cell wall displayed an ultrastructure typical of gram-positive bacteria and consisted of a thin murein layer (50-60 A in thickness); cells exhibited a surface S-layer constituted by large hexagonally packed (p6-symmetry) rod-shaped subunits of 150-160 A in diameter and 200 A in height. In the cytoplasmic membrane, there were intramembrane vesicular structures that sometimes appeared as large leaflets in the central part. The cytoplasm contained numerous vesicular inclusions covered with a monolayered wall, dissimilar to bilamellar lipid membranes. Endospore coats displayed an intricate structure and consisted of three thick layers; the outer layer had an unusual fine structure; the exosporium was also found.
Duda
Vitalii I
VI
Laboratory for structural and functional adaptation of microorganisms, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia.
Suzina
Natalya E
NE
Severina
Lyudmila O
LO
Bogdanova
Tatyana I
TI
Tsaplina
Iraida A
IA
Karavaiko
Gregorii I
GI
D002473:Cell Wall / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D004725:Endospore-Forming Bacteria / Q000166:cytology / Q000648:ultrastructure; D016937:Gram-Positive Endospore-Forming Rods / Q000648:ultrastructure*; D008566:Membranes / Q000648:ultrastructure