Complete sequence and organization of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome.
The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed. The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,879 and 18,274 bp, respectively. The presence and relative positions of 113 genes (76 peptide-encoding genes, 30 tRNA genes, four rRNA genes, and three conserved open reading frames) were identified. The major portion (55.76%) of the C. sativus chloroplast genome consisted of gene-coding regions (49.13% protein coding and 6.63% RNA regions; 27.81% LSC, 9.46% SSC and 18.49% IR regions), while intergenic spacers (including 20 introns) made up 44.24%. The overall G-C content of C. sativus chloroplast genome was 36.95%. Sixteen genes contained one intron, while two genes had two introns. The expansion/contraction manner of IR at IRb/LSC and IR/SSC border in Cucumis was similar to that of Lotus and Arabidopsis, and the manner at IRa/LSC was similar to Lotus and Nicotiana. In total, 56 simple sequence repeats (more than 10 bases) were identified in the C. sativus chloroplast genome.
Kim
Jin-Seog
JS
Biological Function Research Team, Korea Research Institute of Chemical Technology, P.O Box 107, Yuseong, Daejeon 305-600, Korea. jskim@krict.re.kr
Jung
Jong Duk
JD
Lee
Jung-Ae
JA
Park
Hyun-Woo
HW
Oh
Kwang-Hoon
KH
Jeong
Won-Joong
WJ
Choi
Dong-Woog
DW
Liu
Jang Ryol
JR
Cho
Kwang Yun
KY
D002736:Chloroplasts / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018553:Cucumis sativus / Q000166:cytology* / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics; D018745:Genome, Plant; D008969:Molecular Sequence Data