title: A novel technique of three-dimensional reconstruction segmentation and analysis for sliced images of biological tissues.
abstract: A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron microscope slices by Fourier-Bessel synthesis and electron tomography (ET), and a series of computed tomography (CT) was developed to perform simultaneous measurement on the structure and function of biomedical samples. The paper presents the 3D reconstruction segmentation display and analysis results of pollen spore, chaperonin, virus, head, cervical bone, tibia and carpus. At the same time, it also puts forward some potential applications of the new technique in the biomedical realm.
authors.affiliation: Department of Equipments, Kunming General Hospital, Kunming 650032, China. li_jingkm@126.com
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Zhao
authors.forename: Xing-yun
authors.initials: XY
authors.lastname: Ruan
authors.forename: Yong-qing
authors.initials: YQ
authors.lastname: Xu
authors.forename: Wei-zheng
authors.initials: WZ
authors.lastname: Meng
authors.forename: Kun-peng
authors.initials: KP
authors.lastname: Li
authors.forename: Jing-qiang
authors.initials: JQ
authors.lastname: Zhang
mesh_terms: D000465:Algorithms; D019412:Anatomy, Cross-Sectional / Q000379:methods*; D001185:Artificial Intelligence; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity