Cytokine production by whole blood cells: relationship to interleukin gene polymorphism and bone mass.
The aims of this study were to investigate the relationships between the production of interleukin-1 (IL-1), and IL-6 system by whole blood cells, and bone mineral density (BMD), and polymorphisms in IL-1 system and IL-6 gene in postmenopausal Korean women. The production of IL-1alpha, IL-1beta, IL-1 receptor antagonist (IL-1ra), IL-6, and soluble IL-6 receptor (sIL-6r) by lipopolysaccharide-stimulated whole blood cells was measured by ELISA in 110 subjects. Serum osteocalcin, C-telopeptide of type I collagen, and BMD at lumbar spine and proximal femur were measured. IL-1alphaC(-889)T polymorphism, IL-1beta C(-511)T polymorphism, 86-base pair variable number tandem repeat polymorphism in the IL-1ra gene, and IL-6 C(-634)G polymorphism were analyzed. The production of IL-1beta correlated positively with BMD at femoral neck, whereas the production of other ILs did not correlate with BMD at the skeletal sites examined. No significant differences in the production of ILs were observed among normal, osteopenic and osteoporotic postmenopausal women, and among the different IL system polymorphisms groups studied. No correlation between bone turnover markers and the production of ILs was noted. In conclusion IL-1beta may regulate bone metabolism at femoral neck, and the IL system polymorphism do not affect the production of ILs by whole blood cells.
Kim
Jung Gu
JG
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea. kimjg@plaza.snu.ac.kr
Ku
Seung Yup
SY
Lim
Kyung Sil
KS
Jee
Byung Chul
BC
Suh
Chang Suk
CS
Kim
Seok Hyun
SH
Choi
Young Min
YM
Moon
Shin Yong
SY
D000368:Aged; D001773:Blood Cells / Q000187:drug effects / Q000276:immunology; D015519:Bone Density / Q000235:genetics* / Q000276:immunology*; D001851:Bone Diseases, Metabolic / Q000097:blood / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D007378:Interleukins / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000097:blood / Q000235:genetics / Q000276:immunology; D011110:Polymorphism, Genetic; D019947:Receptors, Interleukin-6 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000097:blood / Q000235:genetics
D016207:Cytokines; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D008070:Lipopolysaccharides; D019947:Receptors, Interleukin-6; D012795:Sialoglycoproteins