ATP synthase of chloroplast thylakoid membranes: a more in depth characterization of its ATPase activity.
In contrast to everted mitochondrial inner membrane vesicles and eubacterial plasma membrane vesicles, the ATPase activity of chloroplast ATP synthase in thylakoid membranes is extremely low. Several treatments of thylakoids that unmask ATPase activity are known. Illumination of thylakoids that contain reduced ATP synthase (reduced thylakoids) promotes the hydrolysis of ATP in the dark. Incubation of thylakoids with trypsin can also elicit higher rates of ATPase activity. In this paper the properties of the ATPase activity of the ATP synthase in thylakoids treated with trypsin are compared with those of the ATPase activity in reduced thylakoids. The trypsin-treated membranes have significant ATPase activity in the presence of Ca2+, whereas the Ca2+-ATPase activity of reduced thylakoids is very low. The Mg2+-ATPase activity of the trypsinized thylakoids was only partially inhibited by the uncouplers, at concentrations that fully inhibit the ATPase activity of reduced membranes. Incubation of reduced thylakoids with ADP in Tris buffer prior to assay abolishes Mg2+-ATPase activity. The Mg2+-ATPase activity of trypsin-treated thylakoids was unaffected by incubation with ADP. Trypsin-treated membranes can make ATP at rates that are 75-80% of those of untreated thylakoids. The Mg2+-ATPase activity of trypsin-treated thylakoids is coupled to inward proton translocation and 10 mM sulfite stimulates both proton uptake and ATP hydrolysis. It is concluded that cleavage of the gamma subunit of the ATP synthase by trypsin prevents inhibition of ATPase activity by the epsilon subunit, but only partially overcomes inhibition by Mg2+ and ADP during assay.
McCarty
Richard E
RE
Department of Biology, Johns Hopkins University, Baltimore, Maryland, 21218, USA. rem1@jhu.edu
D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000528:radiation effects; D025222:Chloroplast Proton-Translocating ATPases / Q000737:chemistry* / Q000528:radiation effects; D004789:Enzyme Activation / Q000528:radiation effects; D008027:Light; D010084:Oxidation-Reduction; D018724:Spinacia oleracea / Q000201:enzymology*; D020524:Thylakoids / Q000201:enzymology* / Q000528:radiation effects; D014357:Trypsin / Q000737:chemistry* / Q000528:radiation effects
D014357:Trypsin; D000251:Adenosine Triphosphatases; D025222:Chloroplast Proton-Translocating ATPases