title: Selection of xenobiotic-degrading microorganisms in a biphasic aqueous-organic system.
abstract: Microbial selection on mixtures of chlorinated and nonchlorinated compounds that are poorly soluble in water and/or toxic to growing microbial cells was examined in both biphasic aqueous-organic and monophasic aqueous systems. A biphasic system in which silicone oil was used as the organic phase permitted the acceleration of acclimation, leading to rapid selection and to an increase in xenobiotic compound degradation. In contrast, acclimation, selection, and degradation were very slow in the monophasic aqueous system. The variation in microbial growth rate with the degree of dispersion (i.e., dispersion at different silicone oil concentrations and agitation rates), and cell adhesion to the silicone oil indicate that the performance of the biphasic aqueous-organic system is dependent on the interfacial area between the two phases and that microbial activity is important at this interface. Therefore, the biphasic water-silicone oil system could be used for microbial selection in the presence of xenobiotic compounds that are toxic and have low water solubility.
authors.affiliation: Division des Procédés Biotechnologiques, Département Génie Chimique, Centre de Recherches de Royallieu, B.P. 649, Université de Technologie de Compiègne, 60206 Compiègne Cédex, France.
authors.forename: M
authors.initials: M
authors.lastname: Ascon-Cabrera
authors.forename: J M
authors.initials: JM
authors.lastname: Lebeault