title: Asymbiotic acetylene reduction by a fast-growing cowpea Rhizobium strain with nitrogenase structural genes located on a symbiotic plasmid.
abstract: A procedure was designed which enabled the detection of ex planta nitrogenase activity in the fast-growing cowpea Rhizobium strain IHP100. Nitrogenase activity in agar culture under air occurred at a rate similar to that found for Bradyrhizobium strain CB756 but lower than that for Rhizobium strain ORS571. Hybridization studies showed that both nod and nif genes were located on a 410-kilobase Sym plasmid in strain IHP100.
authors.affiliation: Genetics Department, Research School of Biological Sciences, Australian National University, Canberra, A.C.T. 2601, Australia.
authors.forename: G L
authors.initials: GL
authors.lastname: Bender
authors.forename: J
authors.initials: J
authors.lastname: Plazinski
authors.forename: B G
authors.initials: BG
authors.lastname: Rolfe