title: Vaccination of chicken embryos with escape mutants of La Sota Newcastle disease virus induces a protective immune response.
abstract: To reduce the embryonic pathogenicity of Newcastle disease virus (NDV), escape mutants of the La Sota strain were produced with selected monoclonal antibodies. Immunoselection resulted in the elimination of an epitope by single amino acid substitution (F and HN molecule) or in a conformational change (HN molecule). The embryonic pathogenicity of these escape mutants was reduced and their dose was optimised for in ovo vaccination. Because antibody responses and protection of in ovo vaccinated chicks were similar to controls vaccinated at hatch with the La Sota strain, immunoselection appears a valuable technique to produce attenuated NDV strains, which are candidate in ovo vaccines.
authors.affiliation: Veterinary and Agrochemical Research Centre, Groeselenberg 99, B-1180 Brussels, Belgium. jamas@var.fgov.be
authors.forename: Jan
authors.initials: J
authors.lastname: Mast
authors.forename: Cécile
authors.initials: C
authors.lastname: Nanbru
authors.forename: Mireille
authors.initials: M
authors.lastname: Decaesstecker
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Lambrecht
authors.forename: Bernard
authors.initials: B
authors.lastname: Couvreur
authors.forename: Guy
authors.initials: G
authors.lastname: Meulemans
authors.forename: Thierry
authors.initials: T
authors.lastname: van den Berg
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002642:Chick Embryo / Q000276:immunology* / Q000821:virology; D002645:Chickens / Q000276:immunology*; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005814:Genes, Viral; D015707:HN Protein / Q000032:analysis / Q000235:genetics / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008969:Molecular Sequence Data; D009154:Mutation; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D017422:Sequence Analysis, DNA; D013047:Specific Pathogen-Free Organisms; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D015707:HN Protein; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014613:Vaccines, Attenuated; D014760:Viral Fusion Proteins; D014765:Viral Vaccines