title: [Determination and analysis of the primary structure of NM.BstSEI operone from Bacillus stearothermophilus SE-589 which produces N.BstSEI site-specific nickase].
abstract: Nucleotide sequence of Bacillus stearothermophilus SE-589 DNA fragment which includes an operone for site-specific NM-system with a gene for BstSEI nickase has been determined. Analysis of the regions adjacent to nickase gene has revealed two genes encoding DNA methyltransferases, which belong to different classes. Three genes which form system operone are separated with short open reading frames (ORFs). Analysis of these ORFs has shown that they encode polypeptides which are homologous to different parts of BstSEI nickase, NatB protein and arginase. A difference in GC-content of the beginning and ending regions of the cloned DNA fragment as well as presence of short ORFs similar to genes for known proteins may indicate that NM.BstSEI system operone has evolved by horizonthal DNA transfer.
authors.forename: N S
authors.initials: NS
authors.lastname: Golobova
authors.forename: S S
authors.initials: SS
authors.lastname: Okhapkina
authors.forename: M A
authors.initials: MA
authors.lastname: Abdurashitov
authors.forename: S Kh
authors.initials: SKh
authors.lastname: Degtiarev
mesh_terms: D000595:Amino Acid Sequence; D001119:Arginase / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D015254:DNA Modification Methylases / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004706:Endodeoxyribonucleases / Q000235:genetics*; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D009876:Operon / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D015254:DNA Modification Methylases; D004706:Endodeoxyribonucleases; C103817:N.BstSE nickase; D001119:Arginase