Production and Extracellular Secretion of Aqualysin I (a Thermophilic Subtilisin-Type Protease) in a Host-Vector System for Thermus thermophilus.
Aqualysin I is synthesized as a large precursor, processed, and secreted into the culture medium by Thermus aquaticus YT-1. An expression plasmid for the aqualysin I gene in T. thermophilus HB27 was constructed. T. thermophilus cells harboring the recombinant plasmid produced correctly processed aqualysin I, and the mature enzyme was secreted into the culture medium.
Touhara
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N
Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, and Fermentation Research Institute, Agency of Industrial Science and Technology, Tsukuba Science City, Ibaraki 305, Japan.
Taguchi
H
H
Koyama
Y
Y
Ohta
T
T
Matsuzawa
H
H