Fluorescence analysis of p-hydroxymethamphetamine in urine by thin-layer chromatography.
A highly-sensitive analytical method for the detection of p-hydroxymethamphetamine (pOHMA) in urine is presented. The proposed method combines liquid-liquid extraction with acetonitrile and solid-phase extraction by thin-layer chromatography (TLC) with oxidation using potassium hexacyanoferrate(III) and sodium hydroxide to detect the fluorophor of pOHMA. The detection limit for pOHMA is 10 ng (n = 3). The analysis of pOHMA in forensic samples is successfully performed, without interference from endogenous fluorophors, yielding concentrations in the appropriate range for methamphetamine abusers.
Kato
Noriyuki
N
Scientific Criminal Investigation Laboratory, Kanagawa Prefectural Police Headquarters, Yokohama, Japan. n-katoh@gd5.so-net.ne.jp
Kubo
Hiroaki
H
Homma
Hiroshi
H
D002855:Chromatography, Thin Layer / Q000379:methods*; D015590:Fluorophotometry; D005554:Forensic Medicine; D006801:Humans; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000652:urine; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence / Q000379:methods*
D008694:Methamphetamine; C005877:pholedrine