Enhanced transformation and chemosensitivity of NIH3T3 cells transduced with hepatoma up-regulated protein.
Hepatoma up-regulated protein (HURP) is a recently identified novel cell-cycle-regulated gene. The HURP gene is overexpressed in human hepatocellular carcinoma and transitional cell carcinoma. The cellular function of HURP is not fully understood. In this study, the NIH3T3 cells transduced with the exogenous HURP gene manifested the general characteristics of tumor cells, which had higher growth rate in low-serum media and advanced ability of colony formation on agarose-based plates. Transduced HURP was capable of specifically enhancing the chemosensitivity of deoxycytosine analogs, such as gemcitabine, ARA-C, and 5-AZA-CdR, but neither had an effect on the response of DNA intercalating agents, such as cisplatin, carboplatin, and doxorubicin, nor on the response of microtubule stabilizers, such as paclitaxel, docetaxel, and vinblastine. These results indicate that the HURP gene might be a potential oncogenic gene and capable of enhancing the chemosensitivity of deoxycytosine analogs in NIH3T3 cells.
Wang
Yu-Chun
YC
Department of Medical Research, Chi-Mei Medical Center, Chung Hwa College of Medical Technology, Tainan, Taiwan.
Lee
Ying-Huei
YH
Huang
Guan-Cheng
GC
Lin
Yu-hsuan
YH
Fan-Chiang
Mei Huei
MH
Chiu
Allen W
AW
Huang
Yu-Lun
YL
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007928:Lethal Dose 50; D051379:Mice; D041681:NIH 3T3 Cells; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014161:Transduction, Genetic
D000970:Antineoplastic Agents; C465041:DLGAP5 protein, human; D009363:Neoplasm Proteins; D011994:Recombinant Proteins