title: Improved technique for identification and enumeration of methanogenic bacterial colonies on roll tubes by epifluorescence microscopy.
abstract: Methanogenic fluorescent colonies can be clearly identified on roll tubes by using an epifluorescence microscope equipped with a x 2 objective. Methanogenic and nonmethanogenic colonies could be counted in roll tubes prepared from methanogenic enrichment cultures. Late-developing colonies appearing after 25 days of incubation were mainly methanogenic.
authors.affiliation: Department of Development and Planning, EBARA Corporation, 1-6-27, Khonan, Minato-ku, Tokyo 108, and Chemical Ecology Division, Fermentation Research Institute, Agency of Industrial Science and Technology, MITI, 1-1-3, Higashi, Tsukuba, Ibaraki 305, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kataoka
authors.forename: Y
authors.initials: Y
authors.lastname: Tokiwa
authors.forename: K
authors.initials: K
authors.lastname: Takeda