title: Microbial specificity of metallic surfaces exposed to ambient seawater.
abstract: High-molecular-weight materials associated with the extracellular matrix and film found on titanium and aluminum surfaces after exposure to flowing coastal seawater were isolated. This material was purified by hydroxylapatite chromatography and subsequently employed to produce antibodies in the toad, Bufo marinus. The antibodies were immobilized on a solid support and employed to isolate adhesion-enhancing, high-molecular-weight materials from the laboratory culture media of bacterial strains recovered from the respective metallic surfaces during the course of their exposure to seawater. The adhesion-enhancing materials produced by the surface-associated bacterial strains were immunologically related to the extracellular biofouling matrix material found on the surfaces from which these bacteria were isolated. The surface selectivity of these bacterial strains appeared to be based on the specificity of the interaction between adhesion-enhancing macromolecules produced by these bacteria and the surfaces in question.
authors.affiliation: Department of Marine Sciences, University of Puerto Rico, Mayaguez, Puerto Rico 00708.
authors.forename: B R
authors.initials: BR
authors.lastname: Zaidi
authors.forename: R F
authors.initials: RF
authors.lastname: Bard
authors.forename: T R
authors.initials: TR
authors.lastname: Tosteson