title: [Preliminary study on bacterial culture through near-natural pure culture technique].
abstract: Based on the ecological features of mutual benefits of different microbes in natural environment, near-natural pure culture technique which was an improvement of conventional pure culture method had been put forward. The key point of this new method was to make hole petri dishes covered with millipore membranes. When these covered dishes being used to culture microorganisms, it could provide incubated microbes active materials from native environment, maintain free communicating with outside microbes, and improve their recovery and culturability of some bacteria. To some degree, this technique enhanced their strengths and overcome their weaknesses of mixed culture method and conventional pure culture method so as to increase microbial culturability and even possibly obtain some nonculturable microorganisms.
authors.affiliation: Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, City Construction and Environmental Engineering Academy, Chongqing University, China. yejy@swnu.edu.cn
authors.forename: Jiang-yu
authors.initials: JY
authors.lastname: Ye
authors.forename: Gu-yuan
authors.initials: GY
authors.lastname: Luo
authors.forename: Tu-jin
authors.initials: TJ
authors.lastname: Wang
authors.forename: Hong-ling
authors.initials: HL
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D003470:Culture Media
chemical_list: D003470:Culture Media