title: Cyclooxygenase 1 and 2 expressions in the human thyroid gland.
abstract: We investigated the expression of COX-1 and COX-2 in normal thyroid tissue, follicular adenoma and well-differentiated thyroid carcinomas and evaluated the difference in COX-1 and COX-2 expression. Ten normal thyroid tissues, ten follicular adenomas, ten papillary carcinomas and ten follicular carcinomas were analyzed by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) for expression of COX-1 and COX-2 mRNA. In addition, immunohistochemical staining was performed to find the expression of the two enzymes in normal thyroid tissues and thyroid neoplasia. Expression of COX-1 mRNA in the normal thyroid tissues, follicular adenomas and both well-differentiated carcinomas was similar and weak. However, COX-2 mRNA was strongly expressed in the well-differentiated carcinomas compared to those of normal thyroid tissue and follicular adenoma.
authors.affiliation: Department of Otolaryngology and Head and Neck Surgery, Korea University College of Medicine, Guro Hospital, 97 Gurodong-gil Guro-gu, 152-703, Seoul, Korea.
authors.forename: Heung-Man
authors.initials: HM
authors.lastname: Lee
authors.forename: Seung-Kuk
authors.initials: SK
authors.lastname: Baek
authors.forename: Soon-Young
authors.initials: SY
authors.lastname: Kwon
authors.forename: Kwang-Yoon
authors.initials: KY
authors.lastname: Jung
authors.forename: Sung-Won
authors.initials: SW
authors.lastname: Chae
authors.forename: Soon-Jae
authors.initials: SJ
authors.lastname: Hwang
authors.forename: Jeong-Soo
authors.initials: JS
authors.lastname: Woo
authors.forename: June-Young
authors.initials: JY
authors.lastname: Lee
mesh_terms: D000236:Adenoma / Q000235:genetics / Q000473:pathology; D014408:Biomarkers, Tumor; D001706:Biopsy; D002291:Carcinoma, Papillary / Q000235:genetics / Q000473:pathology; D051545:Cyclooxygenase 1 / Q000235:genetics* / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013961:Thyroid Gland / Q000378:metabolism* / Q000473:pathology; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2