title: Influence of Nutritional Conditions on Production of l-Glutamine by Flavobacterium rigense.
abstract: The nutritional conditions for the production of l-glutamine by Flavobacterium rigense strain 703 were investigated. The optimum concentration of ammonia for achieving the highest yield of l-glutamine (25 mg/ml of broth) was relatively broad, from 0.9 to 1.6%, whereas fumaric acid had a narrow optimum range, near 5.5%. High concentration of inorganic ions such as chloride or sulfate ion clearly inhibited cell growth. Therefore, ammonium salts other than (NH(4))(2)-fumarate were unsuitable for the highest production. The optimum concentration of (NH(4))(2)-fumarate was 7%. To reduce the concentration of fumaric acid in the medium, many substances were evaluated as substitutes. The fumaric acid concentration required for highest l-glutamine yield could not be replaced by any one of the compounds tested. However, part of fumaric acid could be replaced with succinic acid and cupric ion; 4% (NH(4))(2)-fumarate plus 2.5% succinic acid or 5% (NH(4))(2)-fumarate plus 1 mM cupric ion produced results similar to 7% (NH(4))(2)-fumarate in the fermentation medium.
authors.affiliation: Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Company, Ltd., 3-16-89, Kashima, Yodogawa-ku, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nabe
authors.forename: T
authors.initials: T
authors.lastname: Ujimaru
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.forename: I
authors.initials: I
authors.lastname: Chibata