title: The wheat germ cell-free expression system: methods for high-throughput materialization of genetic information.
abstract: This chapter contains protocols for high-throughput protein production based on the cell-free system prepared from eukaryote wheat embryos.
authors.affiliation: Cell-Free Science and Technology Research Center, Ehime University, Matsuyama, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sawasaki
authors.forename: Mudeppa D
authors.initials: MD
authors.lastname: Gouda
authors.forename: Takayasu
authors.initials: T
authors.lastname: Kawasaki
authors.forename: Takafumi
authors.initials: T
authors.lastname: Tsuboi
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Tozawa
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Takai
authors.forename: Yaeta
authors.initials: Y
authors.lastname: Endo
mesh_terms: D002474:Cell-Free System; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary; D006801:Humans; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins; D012333:RNA, Messenger / Q000378:metabolism; D014908:Triticum / Q000196:embryology* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger