Cathepsin B and its interacting proteins, bikunin and TSRC1, correlate with TNF-induced apoptosis of ovarian cancer cells OV-90.
Increasing evidence suggests that lysosomal cysteine proteases cathepsins contribute to the progression of cell apoptosis. Here we found that apoptosis of ovarian cancer cells OV-90 triggered by TNF was cathepsin B-depended. Two cathepsin B binding proteins, bikunin and TSRC1, were identified by yeast two-hybrid method and the interactions were confirmed in vitro and in vivo. Overexpression of bikunin could suppress TNF-induced apoptosis of OV-90 cells, and TSRC1 overexpression had an opposite effect on apoptosis. The presented results suggest that cathepsin B and its interacting proteins, bikunin and TSRC1, are involved in the apoptotic pathway of ovarian cancer cells.
Liu
Jianping
J
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, PR China.
Guo
Qian
Q
Chen
Buxin
B
Yu
Yao
Y
Lu
Hong
H
Li
Yu-Yang
YY
D000071096:ADAMTS Proteins; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D019898:Autocrine Communication / Q000235:genetics; D002401:Cathepsin B / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000378:metabolism*; D019699:Thrombospondins / Q000235:genetics / Q000378:metabolism*; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology*; D020798:Two-Hybrid System Techniques
C486779:ADAMTSL4 protein, human; D008562:Membrane Glycoproteins; C109148:SPINT2 protein, human; D019699:Thrombospondins; D014409:Tumor Necrosis Factor-alpha; D014360:Trypsin Inhibitor, Kunitz Soybean; D002401:Cathepsin B; D000071096:ADAMTS Proteins