[Comparison of the desulfurization activity among several bacteria and analysis of the conservation of their desulfurization genes].
Several bacteria, Bacillus brevis R-6, Pseudomonas delafleldii R-8, Nocardia globerula R-9, Bacillus sphaericus R-16, Rhodococcus erythropolis LSSE8-1 and Gordonia nitida LSSEJ-1, which can convert dibenzothiophene into 2-hydroxybiphenyl and sulfate, were investigated. Desulfurization products were quantitively determined by HPLC. Result revealed that each of these bacteria desulfurize DBT at a different rate. In order to obtain more information, the fragments encoding desulfurizing enzymes were studied. Desulfurization genes of R-6 and R-8 were separately amplified via PCR with specific primers based on the related sequences of Rhodococcus sp. IGTS8. Both sequences areminimally 99% related to IGTS8 sequence. Afterwards, dsz operon of LSSEJ-1 and R-9 were amplified and cloned. Sequences are also highly conservative. Data shows that identity of dszA between R-9 and IGTS8 is 99.6%, and identity of dszA between LSSEJ-1 and IGTS8 is 99.9%; dszB sequence of R-9 and LSSEJ-1 is 99.6% similarity to their counterpart sequence from IGTS8;Identity of dszC between R-9 and IGTS8 is 99.9%, and identity of dszC between LSSEJ-1 and IGTS8 is 99.1% . It may be deduced that the origins of desulfurization genes from mesophilic bacteria are the same.
Xiong
Xiao-chao
XC
Institute of Process Engineering, Chinese Academy of Sciences, Beijing.
Li
Wang-liang
WL
Li
Xin
X
Xing
Jian-min
JM
Liu
Hui-zhou
HZ
D001407:Bacillus / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D033601:Gordonia Bacterium / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000378:metabolism; D012240:Rhodococcus / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*
D013876:Thiophenes; D013455:Sulfur; C016366:dibenzothiophene