title: Redirecting carbon flux in Torulopsis glabrata from pyruvate to alpha-ketoglutaric acid by changing metabolic co-factors.
abstract: The nutrition conditions needed to redirect the carbon flux in Torulopsis glabrata, a pyruvate hyper-production yeast, from pyruvate to alpha-ketoglutaric acid (KG) were investigated in a stirred fermentor. A minor amount of KG (1.3 gl(-1)) was produced when NaOH was used to control the pH, while 12 g KG l(-1) was produced when CaCO(3) was used instead. When thiamine and biotin were included in the medium, 13 g KG l(-1) and 68 g pyruvate l(-1) were produced after 48 h when glucose was nearly consumed (approximately 5 gl(-1)). With fermentation continuing for a further 16 h, the concentration of pyruvate decreased to 31 gl(-1), and KG increased to 30 gl(-1). KG thus accumulated at the expense of pyruvate consumption.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University, Wuxi, PR. China.
authors.forename: H-J
authors.initials: HJ
authors.lastname: Huang
authors.forename: L-M
authors.initials: LM
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: G-C
authors.initials: GC
authors.lastname: Du
authors.forename: J
authors.initials: J
authors.lastname: Chen
mesh_terms: D041221:Candida glabrata / Q000254:growth & development*; D002244:Carbon / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007656:Ketoglutaric Acids / Q000378:metabolism* / Q000494:pharmacology; D019289:Pyruvic Acid / Q000378:metabolism*
chemical_list: D007656:Ketoglutaric Acids; D002244:Carbon; D019289:Pyruvic Acid