title: Transferable DHA-1 cephalosporinase in Escherichia coli.
abstract: Three Escherichia coli isolates resistant to third-generation cephalosporins but negative for extended-spectrum beta-lactamase production were isolated from hospitalised patients in Zagreb, Croatia, during June 2003 to February 2004. Resistance was due to the inducible production of a DHA-1 cephalosporinase. Each isolate contained an integron-associated bla(DHA-1)-ampR sequence carried by similar-sized plasmids, of which one was self-transferable. Serotyping and polymerase chain reaction typing using ERIC2 primer indicated that the isolates were distinct. This is the first description of DHA beta-lactamase production in E. coli.
authors.affiliation: Department of Microbiology, National School of Public Health, Athens, Greece.
authors.forename: P
authors.initials: P
authors.lastname: Giakkoupi
authors.forename: A
authors.initials: A
authors.lastname: Tambic-Andrasevic
authors.forename: S
authors.initials: S
authors.lastname: Vourli
authors.forename: J
authors.initials: J
authors.lastname: Skrlin
authors.forename: S
authors.initials: S
authors.lastname: Sestan-Crnek
authors.forename: L S
authors.initials: LS
authors.lastname: Tzouvelekis
authors.forename: A C
authors.initials: AC
authors.lastname: Vatopoulos
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D018550:Cephalosporin Resistance / Q000235:genetics*; D002510:Cephalosporinase / Q000235:genetics*; D002511:Cephalosporins / Q000494:pharmacology; D003227:Conjugation, Genetic; D017523:Croatia; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D002511:Cephalosporins; D029968:Escherichia coli Proteins; D047090:beta-Lactams; C071526:AmpR protein, Bacteria; D002510:Cephalosporinase; C508952:DHA-1 cephalosporinase, E coli