Resistance to In Vitro Restriction of DNA from Lactic Streptococcal Bacteriophage c6A.
DNA isolated from streptococcal bacteriophage c6A was cut only infrequently by many restriction endonucleases. Fragments of c6A DNA cloned in Escherichia coli plasmids were similarly resistant to cleavage. We conclude that the low frequency of cleavage is due to an unusually low number of restriction enzyme recognition sequences in c6A DNA.
Powell
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The Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria 3052, Australia.
Davidson
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