Antioxidative effects of tetramethylpyrazine on acute ethanol-induced lipid peroxidation.
Acute p.o. administration of 99.5% ethanol (0.1 ml) to fasted mice produced heart toxicity. Pretreatment with p.o. administration of tetramethylpyrazine (TMP) could prevent such toxicity effectively and dose-dependently. The maximal antioxidative effect against 99.5% ethanol-induced heart toxicity could be observed at 1 hour after TMP administration. In order to further investigate the heart protective mechanism of TMP, both lipid peroxidation level in vivo and superoxide scavenging activity were conducted. TMP exhibited a dose-dependently anti-lipid peroxidation effect in mice heart homogenate, and results indicated that 99.5% ethanol-induced intoxicated mice hearts have higher malonic dialdehyde (MDA) levels compared with those in TMP administrated mice hearts. These results suggest that the potentially heart protective mechanism of TMP could be contributed, at least in part, to its prominent anti-lipid peroxidation and anti-free radical formation effects, hence it could protect the heart from lipid peroxidation-induced heart toxicity.
Liu
Chi-Feng
CF
National Taipei College of Nursing, Taipei 112, Taiwan.
Lin
Chia-Hsien
CH
Chen
Chin-Fa
CF
Huang
Tian-Chyuan
TC
Lin
Song-Chow
SC
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002492:Central Nervous System Depressants / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000009:adverse effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009206:Myocardium / Q000378:metabolism; D011719:Pyrazines / Q000494:pharmacology*
D000975:Antioxidants; D002492:Central Nervous System Depressants; D011719:Pyrazines; D000431:Ethanol; D008315:Malondialdehyde; C017953:tetramethylpyrazine