An improved hydrophilicity via electrospinning for enhanced cell attachment and proliferation.
The wettability of electrospun poly(epsilon-caprolactone) (PCL) mats was improved by co-electrospinning with poly(vinyl alcohol) (PVA), by double-spinneret electrospinning method. The improved hydrophilicity of the hybrid PCL/PVA mats was confirmed by water contact angle measurement. The in vitro cell attachment on the hydrophobic PCL and hydrophilically modified PCL/PVA mats was compared by culture studies using human prostate epithelial cells (HPECs). The stability of water-soluble PVA component in the electrospun PCL/PVA mats was checked by thermogravimetric analysis and intensity of fluorescence material after immersion in water for 7 days. The images from scanning electron microscopy, field emission scanning electron microscopy, and optical microscopy showed that the attachment and proliferation rate of HPECs were improved by introducing PVA into the electrospun PCL mats.
Kim
Chi Hun
CH
Department of Textile Engineering, Chonbuk National University, Chonju 561-756, Republic of Korea.
Khil
Myung Seob
MS
Kim
Hak Yong
HY
Lee
Hyun Uk
HU
Jahng
Kwang Yeop
KY
D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004847:Epithelial Cells / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011467:Prostate / Q000648:ultrastructure; D017155:Wettability
D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone; D011142:Polyvinyl Alcohol