title: Purification and properties of hyaluronidase from Palamneus gravimanus (Indian black scorpion) venom.
abstract: Scorpion venoms are a rich source of enzymes. Some of the enzymes such as phospholipase A2, proteolytic enzymes and phosphodiesterase are well characterized. However, hyaluronidase has not been studied extensively. In this paper we describe the purification and characterization of hyaluronidase (Hyaluronate lyase, E.C.3.2.1.35) from the Palamneus gravimanus scorpion venom by a combination of gel filtration on Sephadex G-75 and ion-exchange chromatography on DEAE-cellulose. The optimal pH and temperature for its maximum activity of the isolated enzyme were 4.5 and 37 degrees C, respectively, and its K(m) was 47.61 microg/ml at 37 degrees C and its specific activity was 6411.7 +/- 117TRU/min per mg against 250 +/- 4.0 TRU/min per mg for the whole desiccated venom suggesting 25-fold purification. The molecular weight of the isolated enzyme was 52 +/- 1 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration chromatography on Sephadex G-75. The enzyme was stable for 30 days in the presence of NaCl; no loss of activity was observed up to 37 degrees degrees C and showed a sharp decrease in its activity at 40 degrees C. Heparin inhibited the enzyme activity.
authors.affiliation: Karnatak University's Research Laboratory, Department of Biochemistry, Toxinology Division, Karnatak University Dharwad 580001, Karnataka, India. sunilacr@yahoo.co.in
authors.forename: Sunil S
authors.initials: SS
authors.lastname: Morey
authors.forename: K M
authors.initials: KM
authors.lastname: Kiran
authors.forename: J R
authors.initials: JR
authors.lastname: Gadag
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D004795:Enzyme Stability; D006821:Hyaluronoglucosaminidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012604:Scorpion Venoms / Q000201:enzymology*; D012605:Scorpions / Q000201:enzymology*; D013696:Temperature
chemical_list: D012604:Scorpion Venoms; D006821:Hyaluronoglucosaminidase