Importance of the C-terminal domain of soybean mosaic virus coat protein for subunit interactions.
The potyvirus coat protein (CP) is involved in aphid transmission, cell-to-cell movement and virus assembly, not only by binding to viral RNA, but also by self-interaction or interactions with other factors. In this study, a number of CP mutants of Soybean mosaic virus (SMV) containing deletions and site-directed mutations were generated and cloned into yeast two-hybrid vectors. Interaction was confirmed by the expression of reporter genes, including HIS3, ADE2 and MEL1, in yeast strain AH109. Deletion of the C-terminal region of the CP caused loss of the CP-CP self-interaction ability detected in CP mutants with the C-terminal region. Alanine substitution at the amino acid positions R190, E191, E212, R245, H246 and R249 disrupted CP-CP interaction, whereas substitutions at the amino acid positions R188, D189, D198, K205, K218 and D250 did not. These results indicate that the C-terminal region of SMV CP may contain a domain(s) or amino acids required for CP-CP interaction and virus assembly.
Kang
Sung-Hwan
SH
Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul 151-742, Korea.
Lim
Won-Seok
WS
Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul 151-742, Korea.
Hwang
Sung-Hyun
SH
Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul 151-742, Korea.
Park
Jin-Woo
JW
National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Korea.
Choi
Hong-Soo
HS
National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Korea.
Kim
Kook-Hyung
KH
Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul 151-742, Korea.
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017800:Potyvirus / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013025:Glycine max / Q000821:virology; D020798:Two-Hybrid System Techniques
D036022:Capsid Proteins