title: Phosphotransferase Activity in Clostridium acetobutylicum from Acidogenic and Solventogenic Phases of Growth.
abstract: Clostridium acetobutylicum cells, when energized with fructose, transported and phosphorylated the glucose analog 2-deoxyglucose by a phosphoenolpyruvate-dependent phosphotransferase (PT) system. Butanol up to 2% did not inhibit PT activity, although its chaotropic effect on the cell membrane caused cellular phosphoenolpyruvate and the 2-deoxyglucose-6-phosphate to leak out. Cells harvested from the solventogenic phase of batch growth had a significantly lower PT activity than did cells from the acidogenic phase.
authors.affiliation: Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 02118.
authors.forename: R W
authors.initials: RW
authors.lastname: Hutkins
authors.forename: E R
authors.initials: ER
authors.lastname: Kashket