An immunological assay for detection and enumeration of thermophilic biomining microorganisms.
A specific, fast, and sensitive nonradioactive immunobinding assay for the detection and enumeration of the moderate thermophile Thiobacillus caldus and the thermophilic archaeon Sulfolobus acidocaldarius was developed. It employs enhanced chemiluminescence or peroxidase-conjugated immunoglobulins in a dot or slot blotting system and is very convenient for monitoring thermophilic bioleaching microorganisms in effluents from industrial bioleaching processes.
Amaro
A M
AM
Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Hallberg
K B
KB
Lindström
E B
EB
Jerez
C A
CA