Computational analysis of microRNA targets in Caenorhabditis elegans.
MicroRNAs (miRNAs) are endogenous approximately 22-nucleotide (nt) non-coding RNAs that post-transcriptionally regulate the expression of target genes via hybridization to target mRNA. Using known pairs of miRNA and target mRNA in Caenorhabditis elegans, we first performed computational analysis for specific hybridization patterns between these two RNAs. We counted the numbers of perfectly complementary dinucleotide sequences and calculated the free energy within complementary base pairs of each dinucleotide, observed by sliding a 2-nt window along all nucleotides of the miRNA-mRNA duplex. We confirmed not only strong base pairing within the 5' region of miRNAs (nts 1-8) in C. elegans, but also the required mismatch within the central region (nt 9 or nt 10), and we found weak binding within the 3' region (nts 13-14). We also predicted 687 possible miRNA target transcripts, many of which are thought to be involved in C. elegans development, by combining the above mentioned hybridization tendency with the following analyses: (1) prediction of the miRNA-mRNA duplex with free-energy minimization; (2) identification of the complementary pattern within the miRNA-mRNA duplex; (3) conservation of target sites between C. elegans and C. briggsae, a related soil nematode; and (4) extraction of mRNA candidates with multiple target sites. Rigorous tests using shuffled miRNA controls supported these predictions. Our results suggest that miRNAs recognize their target mRNAs by their hybridization pattern and that many target mRNAs may be regulated through a combination of several specific miRNA target sites in C. elegans.
Watanabe
Yuka
Y
Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, 997-0017, Japan.
Yachie
Nozomu
N
Numata
Koji
K
Saito
Rintaro
R
Kanai
Akio
A
Tomita
Masaru
M
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development; D019295:Computational Biology / Q000379:methods*; D017124:Conserved Sequence; D016208:Databases, Factual; D005786:Gene Expression Regulation; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D012323:RNA Processing, Post-Transcriptional; D018510:RNA, Helminth / Q000737:chemistry / Q000235:genetics; D013816:Thermodynamics; D014158:Transcription, Genetic
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D035683:MicroRNAs; D018510:RNA, Helminth