A nanocompartment system (Synthosome) designed for biotechnological applications.
A nanocompartment system based on two deletion mutants of the large channel protein FhuA (FhuA Delta1-129; FhuA Delta1-160) and an ABA triblock copolymer (PMOXA-PDMS-PMOXA) has been developed for putative biotechnological applications. FhuA is ideally suited for applications in biotechnology due to its monomeric structure, large pore diameter (39-46 A elliptical cross-section) that ensures rapid compound flux, and solved crystallographic structure. Two areas of application were targeted as proof of principle: (A) selective product recovery in nanocompartments and (B) enzymatic conversion in nanocompartments. Selective recovery of negatively charged compounds has been achieved on the example of sulforhodamine B by using positively charged polylysine molecules as trap inside the nanocompartment. Conversion in nanocompartments has been achieved by 3,3',5,5'-tetramethylbenzidine oxidation employing horseradish peroxidase (HRP).
Nallani
Madhavan
M
International University Bremen (IUB), School of Engineering and Science, Campus Ring 8, 28759 Bremen, Germany.
Benito
Samantha
S
Onaca
Ozana
O
Graff
Alexandra
A
Lindemann
Marcus
M
Winterhalter
Mathias
M
Meier
Wolfgang
W
Schwaneberg
Ulrich
U
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D001672:Biocompatible Materials / Q000737:chemistry*; D032701:Biomimetics / Q000379:methods; D001709:Biotechnology / Q000379:methods*; D002070:Butadienes / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics; D008081:Liposomes / Q000737:chemistry*; D008567:Membranes, Artificial; D016297:Mutagenesis, Site-Directed; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry
D001425:Bacterial Outer Membrane Proteins; D001672:Biocompatible Materials; D002070:Butadienes; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; D008081:Liposomes; D008567:Membranes, Artificial; D011994:Recombinant Proteins; D011099:Polyglutamic Acid; C047735:A-B-A triblock copolymer