Immobilisation of DNA to polymerised SU-8 photoresist.
SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalize SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm(2) as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture probes was measured on commercial aldehyde coated glass. DNA probes did not decrease in hybridisation performance after 10 min incubation in water at 98 degrees C prior to hybridisation, indicating a covalent bond between DNA and SU-8. Finally, DNA microarrays of high quality were obtained on SU-8 by contact printing of probe solution directly on SU-8 demonstrating a simple method for the implementation of microarrays in microsystems.
Marie
Rodolphe
R
Department of Micro- and Nano-Technology, Technical University of Denmark, Bldg. 345, DK-2800 Lyngby, Denmark.
Schmid
Silvan
S
Johansson
Alicia
A
Ejsing
Louise
L
Nordström
Maria
M
Häfliger
Daniel
D
Christensen
Claus Bv
CB
Boisen
Anja
A
Dufva
Martin
M
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D010777:Photochemistry / Q000379:methods*; D011108:Polymers / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D011108:Polymers; D004247:DNA