A cyanopropyl reversed phase chromatography enables resolution of huperzine A and B on analytical and preparative scales.
Huperzine A (HupA) and huperzine B (HupB) are two medically important components of Huperzia serrata. It is difficult to obtain high yields of the separation from the plant using conventional liquid extraction and chromatography. However, this study has found that RP chromatography with cyanopropyl (CN) medium was able to separate these two analogs simultaneously from the plant extract with higher resolution. Comparison between a CN medium and a popular C18 medium demonstrated the superiority of the CN over the C18 in resolution for both analytical and preparative separation of HupA and HupB. A preparative process was developed for simultaneous purification of HupA and HupB from H. serrata. The yields on the basis of the mass of the herbal powder for HupA and HupB were 0.019 and 0.008% respectively, which were about 1.9 and 10 times of those reported in the literature.
Li
Xiu-Nan
XN
Department of Pharmaceutical Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, P R China.
Sun
Hai-Hong
HH
Zhang
Gui-Feng
GF
Bi
Jing-Xiu
JX
Ma
Guang-Hui
GH
Ma
Run-Yu
RY
Su
Zhi-Guo
ZG
D000470:Alkaloids / Q000032:analysis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008968:Molecular Conformation; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000032:analysis / Q000737:chemistry; D009570:Nitriles / Q000737:chemistry; D012717:Sesquiterpenes / Q000032:analysis / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*
D000470:Alkaloids; D018696:Neuroprotective Agents; D009570:Nitriles; D012717:Sesquiterpenes; D012997:Solvents; C050426:huperzine A; C053016:huperzine B; D012822:Silicon Dioxide