[HPLC fingerprint for the Corydalis saxicola Bunting injection].
OBJECTIVE
To establish the fingerprint of Corydalis saxicola Bunting injection.


METHODS
It was performed by HPLC Kromasil column was used with acetonitrile and H2O as mobile phase. The flow rate was 0.5 mL x min(-1). The detection wavelength was 254 nm. The mass spectrometry detection was performed on a trap equipped with an ESI interface and operated in positive-ionization mode.


RESULTS
Eleven components of Corydalis saxicola Bunting were identified by LC-MS and 7 of them were quantified.


CONCLUSIONS
The fingerprint chromatogram could represent the characteristics of Corydalis saxicola Bunting injection. Evaluation of resemblances and results of precision were satisfactory. This method could be used as quality and quantity control.
Liu
Si-Ming
SM
Modern Engineering Centerfor Traditional Chinese Medicine, School of Chemistry and Pharmaceutics, East-China University of Science and Technology, Shanghai 200237, China.
Hu
Ping
P
Luo
Guo-An
GA
Wang
Yi-Ming
YM
Chen
Ling-Xin
LX
D001599:Berberine / Q000032:analysis*; D001600:Berberine Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031311:Corydalis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D007267:Injections; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; D001599:Berberine; C005413:palmatine