title: Synthetic Medium for Acetobacter xylinum That Can Be Used for Isolation of Auxotrophic Mutants and Study of Cellulose Biosynthesis.
abstract: Acetobacter xylinum is a bacterium that can synthesize cellulose when grown in an undefined medium containing glucose. We developed a defined minimal medium for A. xylinum that contains 1% glucose, 0.1% NH(4)Cl, 0.115% citric acid, 0.33% Na(2)HPO(4), 0.01% KCl, 0.025% MgSO(4). 7H(2)O, and 7.5 mg of nicotinamide per liter which both allows cellulose synthesis and can be used to isolate a variety of auxotrophic mutants.
authors.affiliation: Department of Biology, University of North Carolina at Greensboro, Greensboro, North Carolina 27412-5001.
authors.forename: E R
authors.initials: ER
authors.lastname: Forng
authors.forename: S M
authors.initials: SM
authors.lastname: Anderson
authors.forename: R E
authors.initials: RE
authors.lastname: Cannon