title: Synthesis of [C]Cellobiose with Clostridium thermocellum Cellobiose Phosphorylase.
abstract: Cellobiose labeled either at the reducing end (4-O-beta-d-glucopyranosyl-d-[U-C]glucopyranose) or at the nonreducing end (4-O-beta-d-[U-C]glucopyranosyl-d -glucopyranose) was synthesized with Clostridium thermocellum cellobiose phosphorylase at greater than 98% purity. The radioactive cellobioses were identical to authentic cellobiose in melting point, optical rotation, isotopic dilution, and chromatographic properties.
authors.affiliation: Central Research and Development Department, Experimental Station, E. I. du Pont de Nemours & Co., Inc., Wilmington, Delaware 19898, and Michigan Biotechnology Center, Michigan State University, East Lansing, Michigan 48824.
authors.forename: T K
authors.initials: TK
authors.lastname: Ng
authors.forename: J G
authors.initials: JG
authors.lastname: Zeikus