title: Characterization of kinetics and products of the Baeyer-Villiger oxygenase MtmOIV, the key enzyme of the biosynthetic pathway toward the natural product anticancer drug mithramycin from Streptomyces argillaceus.
abstract: MtmOIV, the key oxygenase of the mithramycin biosynthetic pathway in Streptomyces argillaceus, was proven to act initially as Baeyer-Villiger monooxygenase, but may also catalyze various follow-up reaction steps. The reaction of the overexpressed pure His6-tagged enzyme with its substrate premithramycin B was studied. Various intermediates and products were isolated and physicochemically characterized, several of them being previously unknown compounds. This is the first example in which a bacterial enzyme was unequivocally proven to act as Baeyer-Villigerase with its natural substrate, that is, in its natural context.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 725 Rose Street, Lexington, Kentucky 40536-0082, USA.
authors.forename: Miranda
authors.initials: M
authors.lastname: Gibson
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nur-e-alam
authors.forename: Fredilyn
authors.initials: F
authors.lastname: Lipata
authors.forename: Marcos A
authors.initials: MA
authors.lastname: Oliveira
authors.forename: Jürgen
authors.initials: J
authors.lastname: Rohr
mesh_terms: D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D007700:Kinetics; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008926:Plicamycin / Q000096:biosynthesis*; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D005182:Flavin-Adenine Dinucleotide; D009249:NADP; D010105:Oxygenases; D008926:Plicamycin