title: Ultrastructural organization of Alicyclobacillus tolerans strain K1T cells.
abstract: Electron microscopy examinations of thin sections and freeze-fracture replicas revealed the specific ultrastructural features of Alicyclobacillus tolerans strain K1(T). In particular, the cell wall displayed an ultrastructure typical of gram-positive bacteria and consisted of a thin murein layer (50-60 A in thickness); cells exhibited a surface S-layer constituted by large hexagonally packed (p6-symmetry) rod-shaped subunits of 150-160 A in diameter and 200 A in height. In the cytoplasmic membrane, there were intramembrane vesicular structures that sometimes appeared as large leaflets in the central part. The cytoplasm contained numerous vesicular inclusions covered with a monolayered wall, dissimilar to bilamellar lipid membranes. Endospore coats displayed an intricate structure and consisted of three thick layers; the outer layer had an unusual fine structure; the exosporium was also found.
authors.affiliation: Laboratory for structural and functional adaptation of microorganisms, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia.
authors.forename: Vitalii I
authors.initials: VI
authors.lastname: Duda
authors.forename: Natalya E
authors.initials: NE
authors.lastname: Suzina
authors.forename: Lyudmila O
authors.initials: LO
authors.lastname: Severina
authors.forename: Tatyana I
authors.initials: TI
authors.lastname: Bogdanova
authors.forename: Iraida A
authors.initials: IA
authors.lastname: Tsaplina
authors.forename: Gregorii I
authors.initials: GI
authors.lastname: Karavaiko
mesh_terms: D002473:Cell Wall / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D004725:Endospore-Forming Bacteria / Q000166:cytology / Q000648:ultrastructure; D016937:Gram-Positive Endospore-Forming Rods / Q000648:ultrastructure*; D008566:Membranes / Q000648:ultrastructure