[Does sepsis impair the healing of colonic anastomosis in splenectomized rats?].
BACKGROUND
To investigate the effect of splenectomy on the healing of colonic anastomoses under normal and septic conditions.


METHODS
Forty Wistar rats were assigned into six groups: group 1: sham, group 2: colonic anastomose, group 3: splenectomy, group 4: colonic anastomose plus sepsis, group 5: colonic anastomose plus splenectomy, group 6: colonic anastomose plus splenectomy plus sepsis. The rats underwent a standardized left colonic resection and primary anastomosis and/or splenectomy. Sepsis was produced by cecal ligation and puncture. Wound healing was evaluated by bursting pressure and hydroxiproline estimates.


RESULTS
Bursting pressures were as follows: group 1: 173 +/- 14 mmHg, group 2: 186 +/- 7 mmHg, group 3: 168 +/- 6 mmHg, group 4: 113 +/- 14 mmHg, group 5: 167 +/- 10 mmHg, and group 6: 183 +/- 3 mmHg. Hidroksiprolin contents were: group 1: 3.5 +/- 0.2 microg/mg, group 2: 3.2 +/- 0.3 microg/mg, group 3: 3.4 +/- 0.2 microg/mg, group 4: 2.3 +/- 0.2 microg/mg, group 5: 3.0 +/- 0.2 microg/mg, grup 6 3.2 +/- 0.1 microg/mg. Statistical significance was found between group 4 and the other groups (p<0.05).


CONCLUSIONS
Sepsis impairs the healing of colonic anastomoses. However, sepsis does not impair the intestinal wound healing in splenectomized rats.
Naycı
Ali
A
Mersin University, Faculty of Medicine Departments of Pediatric Surgery, Mersin, Turkey. anayci@mersin.edu.tr.
Çömelekoğlu
Ülkü
Ü
Çıngı
Eda
E
Taşkınlar
Hakan
H
Avlan
Dinçer
D
Renda
Nurten
N
Aksöyek
Selim
S
D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000737:chemistry / Q000601:surgery*; D004195:Disease Models, Animal; D006909:Hydroxyproline / Q000032:analysis; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000503:physiopathology*; D013156:Splenectomy; D000267:Tissue Adhesions; D014945:Wound Healing / Q000502:physiology*
D006909:Hydroxyproline