Asymptomatic infection by Streptococcus pyogenes in schoolchildren and diagnostic usefulness of antideoxyribonuclease B.
This study is designed to evaluate the immune status of schoolchildren with respect to Streptococcus pyogenes, and to ascertain the usefulness of antideoxyribonuclease B (ADNase B). Antistreptolysin O (ASO) and ADNase B concentrations were measured quantitatively in 266 serum samples from healthy elementary school children in Seoul. Simultaneously, throat cultures were taken in order to isolate S. pyogenes and other beta-hemolytic streptococci (BHS). The upper limits of the normal (ULN) concentration of ASO and ADNase B were 326 IU/mL, and 362 IU/mL, respectively. The correlation between ADNase B (y) and ASO (x) was y = 0.4x+173 (r = 0.46). Mean ADNase B level (392 IU/mL) was significantly higher in children with S. pyogenes than in those with non-group A BHS (236 IU/mL) or no BHS (234 IU/ mL). Some schoolchildren were proven, via ASO and ADNase B tests, to be harboring asymptomatic S. pyogenes infections. The high ULN of ASO and ADNase B in schoolchildren should be carefully considered, in order to interpret the data collected from the patients. We could add the ADNase B test to our set of diagnostic tools, which would allow us to more accurately detect and diagnose streptococcal infections, as ADNase B was more specifically related to the results of throat cultures, and there was little correlation between ASO and ADNase B.
Kim
Sunjoo
S
Department of Laboratory Medicine, Gyeongsang National University School of Medicine, Jinju, Korea.
Lee
Nam Yong
NY
D000907:Antibodies, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000276:immunology; D002648:Child; D003851:Deoxyribonucleases / Q000276:immunology*; D005260:Female; D006801:Humans; D007723:Korea; D008297:Male; D012698:Serologic Tests; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000201:enzymology / Q000276:immunology*; D013301:Streptolysins / Q000276:immunology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D013301:Streptolysins; C025964:streptolysin O; D003851:Deoxyribonucleases; C020069:deoxyribonuclease B