title: Fluorimetric determination of histamine in fish using micellar media and fluorescamine as labelling reagent.
abstract: An analytical method based on the use of fluorescamine to produce a fluorescent derivative with histamine and combined with micellar-enhanced fluorescence detection of the formed complex is developed for the sensitive and rapid determination of histamine in fishes. The fluorescence properties of the obtained complex in water and micellar solutions of sodium dodecyl sulfate (SDS), cetyltrimethylammonium chloride (CTAC) and brij-700 are reported. Physicochemical variables influencing the sensitivity of the method (pH, micellar, fluorescamine and NaCl relative concentrations) have been optimized. The stability of the formed complex, as shown by kinetic study, depends on the pH of the solution. Linear calibration curves allowing an effective histamine determination were established with large linear dynamic range (LDR), and low limits of detection (LOD) between 0.5 and 33 ng mL-1, according to the solvent. Application to the analysis of fish samples (sardines) yielded satisfactory results. The method seems to be suitable for environmental fish quality control.
authors.affiliation: Faculté des Sciences et Techniques, Département de Chimie, Laboratoire de Photochimie et d'Analyse, Université Cheikh Anta Diop, Dakar, Sénégal.
authors.forename: Rabani
authors.initials: R
authors.lastname: Adamou
authors.forename: Atanasse
authors.initials: A
authors.lastname: Coly
authors.forename: Stephy Edgar
authors.initials: SE
authors.lastname: Douabalé
authors.forename: Med Lemine Ould Cheikh Ould
authors.initials: ML
authors.lastname: Saleck
authors.forename: Mame Diabou
authors.initials: MD
authors.lastname: Gaye-Seye
authors.forename: Alphonse
authors.initials: A
authors.lastname: Tine
mesh_terms: D000818:Animals; D005399:Fishes; D005450:Fluorescamine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006632:Histamine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D008823:Micelles; D014867:Water; D005450:Fluorescamine; D006632:Histamine