New Arabidopsis recombinant inbred line populations genotyped using SNPWave and their use for mapping flowering-time quantitative trait loci.
The SNPWave marker system, based on SNPs between the reference accessions Colombia-0 and Landsberg erecta (Ler), was used to distinguish a set of 92 Arabidopsis accessions from various parts of the world. In addition, we used these markers to genotype three new recombinant inbred line populations for Arabidopsis, having Ler as a common parent that was crossed with the accessions Antwerp-1, Kashmir-2, and Kondara. The benefit of using multiple populations that contain many similar markers and the fact that all markers are linked to the physical map of Arabidopsis facilitates the quantitative comparison of maps. Flowering-time variation was analyzed in the three recombinant inbred line populations. Per population, four to eight quantitative trait loci (QTL) were detected. The comparison of the QTL positions related to the physical map allowed the estimate of 12 different QTL segregating for flowering time for which Ler has an allele different from one, two, or three of the other accessions.
el-Lithy
Mohamed E
ME
Laboratory of Genetics, Wageningen University, The Netherlands.
Bentsink
Leónie
L
Hanhart
Corrie J
CJ
Ruys
Gerda J
GJ
Rovito
Daniela
D
Broekhof
José L M
JL
van der Poel
Hein J A
HJ
van Eijk
Michiel J T
MJ
Vreugdenhil
Dick
D
Koornneef
Maarten
M
D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D035264:Flowers / Q000235:genetics* / Q000502:physiology; D008040:Genetic Linkage; D005819:Genetic Markers; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D011995:Recombination, Genetic
D005819:Genetic Markers