Expression of multiple epigenetically regulated cancer/germline genes in nonsmall cell lung cancer.
Cancer/germline (CG) antigens represent promising targets for widely applicable mono- and multiantigen cancer vaccines for nonsmall cell lung cancer (NSCLC). Since little is known about their composite expression in this tumor type, we analyzed 7 CG genes (MAGE-A3, NY-ESO-1, LAGE-1, BRDT, HOM-TES-85, TPX-1 and LDHC) in 102 human NSCLC specimens. About 81% of NSCLC express at least 1 and half of the specimen at least 2 CG genes. Activation of most of these genes occurs more frequently in squamous cell cancer than in adenocarcinomas. Even though we found all genes but one to be regulated by genomic methylation, not all of them are co-expressed. In particular, combining CG genes not localized on the X-chromosome may provide effective treatment for an extended number of patients.
Grunwald
Carolin
C
Department of Internal Medicine III, Johannes Gutenberg-University, Langenbeckstr. 1, 55131 Mainz, Germany, and University Hospital Ghent, Belgium.
Koslowski
Michael
M
Arsiray
Tülin
T
Dhaene
Karl
K
Praet
Marleen
M
Victor
Anja
A
Morresi-Hauf
Alicia
A
Lindner
Michael
M
Passlick
Bernward
B
Lehr
Hans-Anton
HA
Schäfer
Stephan C
SC
Seitz
Gerhard
G
Huber
Christoph
C
Sahin
Ugur
U
Türeci
Ozlem
O
D000951:Antigens, Neoplasm; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling; D005819:Genetic Markers; D018095:Germ-Line Mutation; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D005819:Genetic Markers