title: Kinetic evidence for the uniport mechanism hypothesis in the mitochondrial tricarboxylate transport system.
abstract: The kinetics of the transport of citrate by the tricarboxylate transport system located in the inner mitochondrial membrane was studied in proteoliposomes containing the purified carrier protein, in order to verify the previously hypothesized mechanism of uniport (J. Bioenerg. Biomembr. 35, 133-140, 2003) and achieve some information on the kinetic properties of the carrier transport system. For this purpose, a mathematical model has been elaborated and the experimental data were analyzed according to it. The results indicate that the data actually fit with the uniport model, and hence it is confirmed that the carrier has a single binding site for its substrates and can oscillate between the inside and outside form, in both the free and substrate-bound states. The rearrangement of the free form is slower than the bound form in both directions. The dissociation constants for the internal substrate are at least one order of magnitude higher than the one for external citrate. As a consequence of these last two points, the rate of citrate transport by the carrier is much higher when it operates in exchange with another substrate than when it operates in net uniport.
authors.affiliation: Dipartimento Farmaco-biologico, Università degli Studi di Bari, Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: De Palma
authors.forename: G
authors.initials: G
authors.lastname: Prezioso
authors.forename: V
authors.initials: V
authors.lastname: Scalera
mesh_terms: D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry*; D019343:Citric Acid / Q000737:chemistry*; D003198:Computer Simulation; D004058:Diffusion; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D008930:Mitochondria, Liver / Q000737:chemistry*; D008954:Models, Biological; D008956:Models, Chemical; D051381:Rats
chemical_list: D002352:Carrier Proteins; D008081:Liposomes; C020723:citrate-binding transport protein; D019343:Citric Acid