title: Production and Extracellular Secretion of Aqualysin I (a Thermophilic Subtilisin-Type Protease) in a Host-Vector System for Thermus thermophilus.
abstract: Aqualysin I is synthesized as a large precursor, processed, and secreted into the culture medium by Thermus aquaticus YT-1. An expression plasmid for the aqualysin I gene in T. thermophilus HB27 was constructed. T. thermophilus cells harboring the recombinant plasmid produced correctly processed aqualysin I, and the mature enzyme was secreted into the culture medium.
authors.affiliation: Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, and Fermentation Research Institute, Agency of Industrial Science and Technology, Tsukuba Science City, Ibaraki 305, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Touhara
authors.forename: H
authors.initials: H
authors.lastname: Taguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Koyama
authors.forename: T
authors.initials: T
authors.lastname: Ohta
authors.forename: H
authors.initials: H
authors.lastname: Matsuzawa