Hemolysis caused by surface roughness under shear flow.
In this study, the relationship between the degree of roughness of blood contact surfaces under laminar shear flow conditions and the level of hemolysis resulting from this roughness was investigated using a rotational shear stressor. Unlike previous in vitro experiments that used a pumped circuit, the level of hemolysis was directly evaluated under a constant shear flow. In total, 1.8% of the blood contact area was roughened to an arithmetic mean roughness (Ra) value of between 0.4 and 9.2 microm by machine processing and a shear load was applied for 30 min at a shear flow rate of 3750 s(-1). As a result, the threshold Ra value for the induction of hemolysis was found to be between 0.4 and 0.8 microm. In addition, the results of this experiment suggested that the high shear stress resulting from surface roughness plays a major role in determining the level of hemolysis caused by surface roughness.
Maruyama
Osamu
O
Artificial Organ and Biomaterial Group, Institute for Human Science and Biomedical Engineering, National Institute of Advanced Industrial Science and Technology (AIST), 1-2-1 Namiki, Tsukuba, Ibaraki 305-8564, Japan. osamu.maruyama@aist.go.jp
Numata
Yusuke
Y
Nishida
Masahiro
M
Yamane
Takashi
T
Oshima
Ikuya
I
Adachi
Yoshikazu
Y
Masuzawa
Toru
T
D000818:Animals; D002417:Cattle; D004867:Equipment Design; D006354:Heart, Artificial / Q000009:adverse effects; D006461:Hemolysis; D066298:In Vitro Techniques; D013314:Stress, Mechanical; D013499:Surface Properties