Asymbiotic acetylene reduction by a fast-growing cowpea Rhizobium strain with nitrogenase structural genes located on a symbiotic plasmid.
A procedure was designed which enabled the detection of ex planta nitrogenase activity in the fast-growing cowpea Rhizobium strain IHP100. Nitrogenase activity in agar culture under air occurred at a rate similar to that found for Bradyrhizobium strain CB756 but lower than that for Rhizobium strain ORS571. Hybridization studies showed that both nod and nif genes were located on a 410-kilobase Sym plasmid in strain IHP100.
Bender
G L
GL
Genetics Department, Research School of Biological Sciences, Australian National University, Canberra, A.C.T. 2601, Australia.
Plazinski
J
J
Rolfe
B G
BG