Construction of tn3-containing plasmids from plant-pathogenic pseudomonads and an examination of their biological properties.
Indigenous plasmids isolated from Pseudomonas tabaci ATCC 11528(pJP1), Pseudomonas angulata 45(pJP30), and P. tabaci BR2(pBPW1) (M. Obukowicz and P. D. Shaw, J. Bacteriol. 155:438-442, 1983) were labeled with Tn3, and the strains were subsequently cured of their respective plasmids. Plasmid-containing and cured isolates caused plant symptoms that were nearly indistinguishable, and the same amount of tabtoxin was produced by P. tabaci strains ATCC 11528 and BR2.
Obukowicz
M
M
Department of Plant Pathology, University of Illinois, Urbana, Illinois 61801.
Shaw
P D
PD