Expression of m157, a murine cytomegalovirus-encoded putative major histocompatibility class I (MHC-I)-like protein, is independent of viral regulation of host MHC-I.
A murine cytomegalovirus (MCMV)-encoded protein, m157, has a putative major histocompatibility complex class I (MHC-I) structure and is recognized by the Ly49H NK cell activation receptor. Using a monoclonal antibody against m157, in this study we directly demonstrated that m157 is a cell surface-expressed glycophosphatidylinositol-anchored protein with early viral gene kinetics. Beta-2 microglobulin and TAP1 (transporter associated with antigen processing 1) were not required for its expression. MCMV-encoded proteins that down-regulate MHC-I did not affect the expression of m157. Thus, m157 is expressed on infected cells in a manner independent of viral regulation of host MHC-I.
Tripathy
Sandeep K
SK
Washington University School of Medicine, Gastroenterology Division, Department of Medicine, St. Louis, MO 63110, USA.
Smith
Hamish R C
HR
Holroyd
Erika A
EA
Pingel
Jeanette T
JT
Yokoyama
Wayne M
WM
D000818:Animals; D015967:Gene Expression Regulation, Viral; D006566:Herpesviridae Infections / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D018146:Muromegalovirus / Q000737:chemistry* / Q000276:immunology
D015395:Histocompatibility Antigens Class I