Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors.
A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.
Khazak
Vladimir
V
NexusPharma, Langhorne, PA, USA.
Golemis
Erica A
EA
Weber
Lutz
L
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D006801:Humans; D010957:Plasmids; D025941:Protein Interaction Mapping; D019908:Proto-Oncogene Proteins c-raf / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D018631:ras Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*
D000935:Antifungal Agents; D019908:Proto-Oncogene Proteins c-raf; D018631:ras Proteins