[bcl-2 expression in small cell lung cancer: a mechanism for apoptosis antagonism and immune evasion].
OBJECTIVE
To observe bcl-2 expression and its correlation with apoptosis antagonism and immune evasion of small cell lung cancer cell line and explore the application of bcl-2 antisense thio-oligonucleotide (bcl-2 SON) in gene therapy for lung cancer.


METHODS
Western blotting was performed to detect bcl-2 expression in NCI-H69 cell line expressing Bcl-2 protein treated with bcl-2 SON. The treated NCI-H69 cells, along with the cells without bcl-2 SON treatment and NCI-H82 cells that did not express Bcl-2 protein, were respectively co-cultured with tumor-infiltrating lymphocytes (TILs) isolated from fresh tumor samples, and the apoptosis of the lung cancer cells was assessed by JAM assay.


RESULTS
Western blotting revealed obvious inhibition of bcl-2 expression in NCI-H69 cells in response to bcl-2 SON treatment. JAM assay showed that the apoptosis of bcl-2 SON-treated H69 cells and NCI-H82 cells increased with the elevation of TIL ratio in the co-culture, while such changes were not observed in NCI-H69 cells without bcl-2 SON treatment.


CONCLUSIONS
Small cell lung cancer cells expressing bcl-2 may antagonize the antitumor immune attack in the host, and bcl-2 SON may provide an effective alternative in gene therapy for small cell lung cancer.
Zheng
Jun-meng
JM
Department of Cardiothoracic Surgery, People's Hospital of Zhongshan, Zhongshan 528403, China.
Wang
De-kun
DK
Jiang
Hai-ming
HM
D000328:Adult; D017209:Apoptosis / Q000502:physiology; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D018920:Coculture Techniques; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000166:cytology; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D019253:Proto-Oncogene Proteins c-bcl-2