title: Genomic structure and transcriptional studies on the mouse ribosomal protein S3 gene: expression of U15 small nucleolar RNA.
abstract: Ribosomal protein S3 (rpS3) is a multifunctional ribosomal protein (RP) which is known to function as a DNA repair endonuclease as well as an RP. Recently, it was reported that rpS3 is involved in apoptosis. We identified the complete 4760 base pair genomic structure of the mouse rpS3 gene, which is composed of 7 exons and 6 introns. Promoter study revealed that transcription of the mouse rpS3 gene started at two C residues embedded in the 5'-terminal oligopyrimidine tract (5'-TOP); this was then compared with the human counterpart. Functional U15 small nucleolar RNAs (snoRNAs) were expressed from the first and the fifth introns. About 300 base pairs (bps) upstream of the 5'-untranslated region (5'-UTR) of the mouse rpS3 gene was sufficient to show maximum transcription activity. This report shows the conservation of the genomic structure of the rpS3 gene in vertebrates and characteristics of its promoter similar to those of promoters of other mammalian RPs.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Seong Min
authors.initials: SM
authors.lastname: Lee
authors.forename: Mijin
authors.initials: M
authors.lastname: Kim
authors.forename: Eun Pyo
authors.initials: EP
authors.lastname: Moon
authors.forename: Byeong Jae
authors.initials: BJ
authors.lastname: Lee
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Joon
authors.initials: J
authors.lastname: Kim
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics / Q000378:metabolism; D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D005091:Exons / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D051379:Mice; D008845:Microinjections; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009865:Oocytes / Q000378:metabolism; D020537:RNA, Small Nucleolar / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014981:Xenopus / Q000235:genetics / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D020537:RNA, Small Nucleolar; D012269:Ribosomal Proteins; C044152:ribosomal protein S3