Herpesviral protein networks and their interaction with the human proteome.
The comprehensive yeast two-hybrid analysis of intraviral protein interactions in two members of the herpesvirus family, Kaposi sarcoma-associated herpesvirus (KSHV) and varicella-zoster virus (VZV), revealed 123 and 173 interactions, respectively. Viral protein interaction networks resemble single, highly coupled modules, whereas cellular networks are organized in separate functional submodules. Predicted and experimentally verified interactions between KSHV and human proteins were used to connect the viral interactome into a prototypical human interactome and to simulate infection. The analysis of the combined system showed that the viral network adopts cellular network features and that protein networks of herpesviruses and possibly other intracellular pathogens have distinguishing topologies.
Uetz
Peter
P
Institut für Genetik, Forschungszentrum Karlsruhe, Postfach 3640, Karlsruhe, D-76021 Germany.
Dong
Yu-An
YA
Zeretzke
Christine
C
Atzler
Christine
C
Baiker
Armin
A
Berger
Bonnie
B
Rajagopala
Seesandra V
SV
Roupelieva
Maria
M
Rose
Dietlind
D
Fossum
Even
E
Haas
Jürgen
J
D002460:Cell Line; D003001:Cloning, Molecular; D014645:Herpesvirus 3, Human / Q000378:metabolism*; D019288:Herpesvirus 8, Human / Q000378:metabolism*; D006801:Humans; D016366:Open Reading Frames; D011485:Protein Binding; D020543:Proteome / Q000378:metabolism*; D049490:Systems Biology; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
D020543:Proteome; D014764:Viral Proteins