[Simultaneous determination of five groups of components in qingkailing injection by high performance liquid chromatography with photo diode array detector and evaporative light scattering detector].
A method was established for the simultaneous quantification of nine components of five different structural types in Qingkailing injection. High performance liquid chromatography coupled with a photo diode array detector and an evaporative light scattering detector (HPLC-DAD-ELSD) was employed in the determination. Four monitoring wavelengths of 240, 254, 280 and 330 nm were set to determine nucleosides (uridine and adenosine), iridoid glucoside (geniposide), flavone glycoside (baicalin) and organic acids (chlorogenic acid and caffeic acid) respectively, and a combined evaporative light scattering detector was used to detect three steroid compounds (cholic acid, ursodesoxycholic acid and hyodeoxycholic acid). The proposed method permitted the simultaneous separation and determination of five groups of compounds in Qingkailing injection, and acceptable validation results of the precision, repeatability, stability and accuracy tests were achieved. The method was applied to the analysis of 19 Qingkailing injection samples from three different plants, and the results indicated that the method could be used as a convenient and reliable method in the multi-component determination and quality control of traditional Chinese medicines.
Yan
Shikai
S
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Xin
Wenfeng
W
Luo
Guoan
G
Wang
Yiming
Y
Cheng
Yiyu
Y
D000241:Adenosine / Q000032:analysis / Q000737:chemistry; D002109:Caffeic Acids / Q000032:analysis / Q000737:chemistry; D002726:Chlorogenic Acid / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D039823:Iridoids / Q000032:analysis / Q000737:chemistry; D008516:Medicine, Chinese Traditional; D012542:Scattering, Radiation; D014529:Uridine / Q000032:analysis / Q000737:chemistry
D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D039823:Iridoids; C504928:Qingkailing; C007835:geniposide; D002726:Chlorogenic Acid; C038044:baicalin; D000241:Adenosine; C040048:caffeic acid; D014529:Uridine