Role of peroxynitrite anion in renal hypothermic preservation injury.
BACKGROUND
Peroxynitrite anions may play a role in normothermic renal ischemia and reperfusion. The purpose of this study was to determine if endogenous peroxynitrite anion is involved in renal preservation injury.


METHODS
Experiments were conducted in isolated canine renal tubules and in a canine autotransplant model of hypothermic preservation injury.


RESULTS
Isolated renal tubules demonstrated progressive loss of membrane transport function after reperfusion with increasing cold storage times in UW solution as assessed by tetraethylammonium cation transport (TEA). This transport defect was not altered by reperfusion in the presence of WW85, a peroxynitrite decomposition catalyst. Likewise, tubule LDH release was not altered by WW85. Renal tubules did not demonstrate any evidence of peroxynitrite formation after cold storage (0-120 h) or after subsequent reperfusion in vitro as measured by nitrotyrosine adduct formation. Addition of exogenous peroxynitrite (1 mM) directly to freshly isolated renal tubules produced strong nitrotyrosine signals but failed to alter membrane function (TEA uptake). Conversely, SIN-1, a peroxynitrite generator molecule, failed to produce a nitrotyrosine signal in extracted renal tubule proteins but significantly impaired transport function. Finally, function of cold stored canine autografts was not affected by the scavenging of peroxynitrite anions (WW85) before kidney harvest and immediately at reperfusion. Tissue biopsies from cold stored kidney autografts also failed to show evidence of peroxynitrite synthesis either after cold storage (72 h) or after kidney transplantation (60 min reperfusion).


CONCLUSIONS
This study concludes that peroxynitrite anions are not formed and are not involved in renal preservation injury.
Mangino
Martin J
MJ
Department of Surgery, University of Wisconsin Medical School, Madison, WI, USA. mjmangino@vcu.edu
Ametani
Mary S
MS
Gilligan
Barbara J
BJ
Szabo
Csaba
C
Brounts
Lionel
L
Southard
James H
JH
D000818:Animals; D003404:Creatinine / Q000097:blood; D015925:Cryopreservation; D004285:Dogs; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology; D016030:Kidney Transplantation; D007684:Kidney Tubules / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009926:Organ Preservation / Q000009:adverse effects*; D030421:Peroxynitrous Acid / Q000096:biosynthesis* / Q000627:therapeutic use / Q000633:toxicity; D015427:Reperfusion Injury / Q000503:physiopathology*
C506898:WW85 compound; D030421:Peroxynitrous Acid; D003404:Creatinine; D007770:L-Lactate Dehydrogenase