title: [Ultracytochemical localization of enzymes and DNA analysis during the development of garlic].
abstract: It is believed that during storage, the parenchyma cells of garlic (Allium sativum L.) bulb would wither and fade gradually, and nutrients released inside the cells become available for the germination and growth of the young bud. In this study, The distributions of acid phosphatase (APase) and Adenosine Triphosphatase (ATPase) during germination were analyzed based on the method of lead precipitation at the electron microscopic level. It was found that their activities presenting in plasma membrane, cell wall and plasmodesma increased along the different developmental periods during storage. The fact that the most intensive enzymatic activity of APase and ATPase appeared at germination indicates that degradation, transformation and exportation of cell matrix are helpful in complete translocation of nutrient to new bud. The DNA in the degrading parenchyma cells was analyzed using the agarose electrophoresis. Results clearly showed a typical DNA ladder on the gel, indicating that gene-controlled, programmed cell death may contribute to the degradation of garlic parenchyma cells.
authors.affiliation: College of Life Sciences ,Hebei Agriculture University, Baoding.
authors.forename: Feng Ju
authors.initials: FJ
authors.lastname: Wei
authors.forename: Ying Ying
authors.initials: YY
authors.lastname: Zhang
authors.forename: Chun Yan
authors.initials: CY
authors.lastname: Hou
authors.forename: Sheng Fang
authors.initials: SF
authors.lastname: Han
authors.forename: Dong Mei
authors.initials: DM
authors.lastname: Wang
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D018744:DNA, Plant / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism*; D005737:Garlic / Q000201:enzymology* / Q000235:genetics* / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D046529:Microscopy, Electron, Transmission; D010940:Plant Proteins / Q000378:metabolism*
chemical_list: D018744:DNA, Plant; D004798:Enzymes; D010940:Plant Proteins; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases