title: Differential synthesis of two interleukin-1 receptor antagonist variants and interleukin-8 by peripheral blood neutrophils.
abstract: With a short lifespan and containing only few ribosomes and endoplasmic reticulum structures, neutrophils are thought to have a limited capacity for protein synthesis. We here show that peripheral blood polymorphonuclear neutrophils (PMN) are able react to stimulants with differential production of two interleukin (IL)-1 receptor antagonist (IL-1ra) isoforms, secreted IL-1ra (sIL-1ra) and the 16kDa intracellular form of IL-1ra (icIL-1ra3), as well as IL-8. Neutrophils of a high purity and with a low degree of preactivation upregulate mRNA and de novo synthesize protein of both IL-1ra variants and IL-8 in response to granulocyte-macrophage colony-stimulating factor and lipopolysaccharide. The cytokines are differentially regulated and distributed in two intracellular compartments. In comparison with peripheral blood mononuclear cells (PBMC), PMN produce distinctly more sIL-1ra but significantly less IL-8. This may indicate an anti-inflammatory role, enabling PMN to antagonize proinflammatory signals. It is therefore possible that PMN play an important role in immune regulation by counteracting a dysregulation of the inflammatory process.
authors.affiliation: Institute of Immunology, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074 Aachen, Germany.
authors.forename: Anja K
authors.initials: AK
authors.lastname: Schröder
authors.forename: Maren
authors.initials: M
authors.lastname: von der Ohe
authors.forename: Daniela
authors.initials: D
authors.lastname: Fleischer
authors.forename: Lothar
authors.initials: L
authors.lastname: Rink
authors.forename: Peter
authors.initials: P
authors.lastname: Uciechowski
mesh_terms: D014644:Genetic Variation; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis*; D009504:Neutrophils / Q000737:chemistry* / Q000276:immunology*; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D017472:Receptors, Interleukin-1 / Q000276:immunology*; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000235:genetics; D013347:Subcellular Fractions / Q000737:chemistry
chemical_list: C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D016209:Interleukin-8; D020033:Protein Isoforms; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins