title: Expression of m157, a murine cytomegalovirus-encoded putative major histocompatibility class I (MHC-I)-like protein, is independent of viral regulation of host MHC-I.
abstract: A murine cytomegalovirus (MCMV)-encoded protein, m157, has a putative major histocompatibility complex class I (MHC-I) structure and is recognized by the Ly49H NK cell activation receptor. Using a monoclonal antibody against m157, in this study we directly demonstrated that m157 is a cell surface-expressed glycophosphatidylinositol-anchored protein with early viral gene kinetics. Beta-2 microglobulin and TAP1 (transporter associated with antigen processing 1) were not required for its expression. MCMV-encoded proteins that down-regulate MHC-I did not affect the expression of m157. Thus, m157 is expressed on infected cells in a manner independent of viral regulation of host MHC-I.
authors.affiliation: Washington University School of Medicine, Gastroenterology Division, Department of Medicine, St. Louis, MO 63110, USA.
authors.forename: Sandeep K
authors.initials: SK
authors.lastname: Tripathy
authors.forename: Hamish R C
authors.initials: HR
authors.lastname: Smith
authors.forename: Erika A
authors.initials: EA
authors.lastname: Holroyd
authors.forename: Jeanette T
authors.initials: JT
authors.lastname: Pingel
authors.forename: Wayne M
authors.initials: WM
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D015967:Gene Expression Regulation, Viral; D006566:Herpesviridae Infections / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D018146:Muromegalovirus / Q000737:chemistry* / Q000276:immunology
chemical_list: D015395:Histocompatibility Antigens Class I