title: [Detection of Escherichia coli in wastewater based on enzyme immunoassay].
abstract: This research described the fast detection method based on ELISA for the E. coli in drain of wastewater treatment plants. The optimized conditions, such as reaction format (sandwich or direct), the concentration of HRP-E. coli conjugate, anti-HPR antibody and pretreatment of E. coli, were studied. Those results showed that the linear range of detection for E. coli was 10 CFU/ mL - 6 x 10(4) CFU/mL. This method shows the potential ability for the technics control of wastewater treatment plants. Comparing with conventional methods, it is a convenient and sensitive detection method with low cost.
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Xi
authors.forename: Qiang
authors.initials: Q
authors.lastname: Cai
authors.forename: Miao
authors.initials: M
authors.lastname: He
authors.forename: Han-Chang
authors.initials: HC
authors.lastname: Shi
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000302:isolation & purification*; D014865:Waste Disposal, Fluid / Q000379:methods*; D014871:Water Microbiology