An engineered microenvironment for multidimensional microscopy of live cells.
Multidimensional imaging (MD) of live cells is gaining importance in biomedical research as the commercial availability of confocal, nonlinear optical microscopes, environmental chambers, and specific fluorescence probes grows. One crucial aspect of the MD live cell imaging involves the proper immobilization of cells, which refers to the rapid and sufficient immobilization of cells on the microscope stage, neither disrupting the cellular structure and functions nor affecting the optical properties of the cells and the environments. Conventional cell immobilization methods glue the anchoring cells to coated surfaces, but such methods require centrifugation or extended incubation and are not suitable for cells in suspension. Most of the current three-dimensional (3-D) gels either exhibit unsatisfactory optical properties or have adverse effects on cell functions in culture. Recently, an engineered 3-D microcapsule has been developed that involves the complex coacervation of a positively charged collagen and a negatively charged polymer of 2-hydroxyethyl methacrylate--methacrylic acid--methyl methacrylate (HEMA-MMA-MAA). Hence, confocal imaging of live cells in this engineered 3-D microenvironment was investigated for its optical properties and cellular function compatibility. We report here that this microenvironment facilitates efficient cell immobilization, exhibits good optical properties, and can preserve cellular structures and functions, which will be useful in MD imaging of live cells for various applications.
Lin
Pao-Chun
PC
National University Medical Institutes and Department of Physiology, National University of Singapore.
Cheng
Ping-Chin
PC
Yu
Hanry
H
D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D048430:Cell Shape; D002477:Cells / Q000166:cytology* / Q000648:ultrastructure; D018914:Cells, Immobilized / Q000166:cytology* / Q000648:ultrastructure; D003094:Collagen; D022781:Hepatocytes / Q000166:cytology; D008689:Methacrylates; D020366:Methylmethacrylate; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D041681:NIH 3T3 Cells; D011109:Polymethacrylic Acids; D051381:Rats
D008689:Methacrylates; D011109:Polymethacrylic Acids; D020366:Methylmethacrylate; C008384:methacrylic acid; C005044:hydroxyethyl methacrylate; D003094:Collagen