title: Spectrophotometric determination of tranexamic acid in pharmaceutical bulk and dosage forms.
abstract: In this study, a simple, fast, accurate and sensitive spectrophotometric method has been developed for the determination of tranexamic acid in bulk and pharmaceutical preparations. The method is based on the reaction of ninhydrin with the primary amino group of tranexamic acid in the basic medium at pH 8.0. The reaction produces a bluish-purple color which absorbs maximally at 565 nm. Beer's law was obeyed in the range of 3-40 microg ml(-1) with molar absorptivity of 5.093 x 10(3) L mol(-1) cm(-1). The effects of various factors such as temperature, heating time, concentration of reagent, color stability and interferences were investigated to optimize the procedure. The results have been validated analytically and statistically. The proposed method has been applied for the determination of tranexamic acid in bulk and pharmaceutical preparations with good results.
authors.affiliation: Department of Chemistry, Bahauddin Zakariya University, Multan, Pakistan. drtariq2000@hotmail.com
authors.forename: Tariq Mahmood
authors.initials: TM
authors.lastname: Ansari
authors.forename: Asad
authors.initials: A
authors.lastname: Raza
authors.forename: Atta-ur
authors.initials: AU
authors.lastname: Rehman
mesh_terms: D004304:Dosage Forms; D015394:Molecular Structure; D009555:Ninhydrin / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods; D013997:Time Factors; D014148:Tranexamic Acid / Q000032:analysis*
chemical_list: D004304:Dosage Forms; D004364:Pharmaceutical Preparations; D014148:Tranexamic Acid; D009555:Ninhydrin