Chitin induces natural competence in Vibrio cholerae.
The mosaic-structured Vibrio cholerae genome points to the importance of horizontal gene transfer (HGT) in the evolution of this human pathogen. We showed that V. cholerae can acquire new genetic material by natural transformation during growth on chitin, a biopolymer that is abundant in aquatic habitats (e.g., from crustacean exoskeletons), where it lives as an autochthonous microbe. Transformation competence was found to require a type IV pilus assembly complex, a putative DNA binding protein, and three convergent regulatory cascades, which are activated by chitin, increasing cell density, and nutrient limitation, a decline in growth rate, or stress.
Meibom
Karin L
KL
Division of Infectious Diseases and Geographic Medicine, Department of Microbiology and Immunology, and Stanford Institute for the Environment, Stanford University, Stanford, CA 94305, USA. kmeibom@necker.fr
Blokesch
Melanie
M
Dolganov
Nadia A
NA
Wu
Cheng-Yen
CY
Schoolnik
Gary K
GK
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018441:Biofilms / Q000254:growth & development; D003386:Brachyura / Q000382:microbiology; D002686:Chitin / Q000378:metabolism / Q000502:physiology*; D003470:Culture Media; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D036781:Fimbriae Proteins / Q000096:biosynthesis / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000378:metabolism; D016368:Frameshift Mutation; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008954:Models, Biological; D009154:Mutation; D010641:Phenotype; D018085:Regulon; D012808:Sigma Factor / Q000378:metabolism; D014169:Transformation, Bacterial; D014734:Vibrio cholerae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D041241:Vibrio cholerae O1 / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000502:physiology
D001426:Bacterial Proteins; D003470:Culture Media; D004268:DNA-Binding Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D002686:Chitin; D036781:Fimbriae Proteins