Label-free, real-time glycosyltransferase assay based on a fluorescent artificial chemosensor.
Wongkongkatep
Jirarut
J
Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Katsura, Kyoto 615-8510, Japan.
Miyahara
Yoshifumi
Y
Ojida
Akio
A
Hamachi
Itaru
I
D015374:Biosensing Techniques; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004789:Enzyme Activation; D005456:Fluorescent Dyes / Q000737:chemistry*; D016695:Glycosyltransferases / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009711:Nucleotides / Q000138:chemical synthesis / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship; D015032:Zinc / Q000737:chemistry*
D005456:Fluorescent Dyes; D009711:Nucleotides; D009942:Organometallic Compounds; D016695:Glycosyltransferases; D015032:Zinc