[Load of calcium probe Fura -2/AM in Escherichia coli cells].
The fluorescence characteristics of Fura-2/AM, a kind of calcium probe normally used in analysis of animal cells, were studied in detail in this work. The load of the probe into Escherichia coli cells was elucidated through fluorescence spectra. The combination of Fura-2 with calcium ions inside Escherichiacoli cells was observed, and the transmembrane behaviors of extracellular calcium to Escherichia coli cells were also investigated. The work was aimed at further researches on the calcium-induced horizontal gene transfer in Escherichia coli cells.
Shao
Mei
M
College of Chemistry and Molecular Sciences, Wuhan University, China.
Wang
Hong-mei
HM
Liu
Zhi-hong
ZH
Shen
Ping
P
Cai
Rui-xiu
RX
D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D016257:Fura-2; D013050:Spectrometry, Fluorescence
D002118:Calcium; D016257:Fura-2