Simple, sensitive zymogram technique for detection of xylanase activity in polyacrylamide gels.
A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands corresponding to enzymatic activity against an opaque background.
Royer
J C
JC
Department of Environmental and Forest Biology, College of Environmental Science and Forestry, State University of New York, Syracuse, New York 13210.
Nakas
J P
JP