Identification of sodium channel mutations in human head louse (Anoplura: Pediculidae) from Denmark.
The presence of the two mutations T9291-L932F in the voltage-sensitive sodium channel gene associated with permethrin resistance (kdr-like) was shown in head louse, Pediculus humanus capitis De Geer, populations in Denmark. The existence of one susceptible and one T929I-L932F haplotype based on the limited single nucleotide polymorphism (SNP) of these sequences was established. One louse had an SNP causing a G943A substitution in the trans-membrane segment five of domain II on the sodium channel, which has not been identified in other insect species. A polymerase chain reaction-restriction endonuclease method using genomic DNA to discriminate between resistant and susceptible alleles in head lice was developed and implemented. The SNP that results in the T9291 substitution also creates a cutting site for the restriction endonuclease SspI, and the presence of one or two SspI cutting sites in head lice is diagnostic for the T9291-L932F haplotype.
Kristensen
Michael
M
Danish Pest Infestation Laboratory, Danish Institute of Agricultural Science, Skovbrynet 14, 2800 Kgs. Lyngby, Denmark.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004262:DNA Restriction Enzymes / Q000378:metabolism; D003718:Denmark; D006239:Haplotypes / Q000235:genetics; D007305:Insecticide Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010374:Pediculus / Q000235:genetics*; D026023:Permethrin; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015222:Sodium Channels / Q000235:genetics*
D017931:DNA Primers; D015222:Sodium Channels; D026023:Permethrin; D004262:DNA Restriction Enzymes