title: A simple, rapid method for enumerating total viable and metabolically active bacteria in groundwater.
abstract: We report here a new staining procedure which uses both the enzymatic dehydrogenation of 2-(p-iodophenyl)-3-p-(nitrophenyl)-5 phenyltetrazolium chloride to a pink intracellular formazan and the DNA-specific fluorochrome 4',6'-diamidino-2-phenylindole. Application of this staining procedure to cells concentrated on filters and then transferred to microscope slides by the filter-transfer-freeze technique has proven valuable for statistically accurate enumeration of the total viable and metabolically active cells in groundwaters.
authors.affiliation: Department of Biology, Virginia Polytechnic Institute & State University, Blacksburg, Virginia 24061.
authors.forename: L K
authors.initials: LK
authors.lastname: King
authors.forename: B C
authors.initials: BC
authors.lastname: Parker