A novel spectrofluorimetric method for the determination of DNA.
A new simple, selective and sensitive fluorescence quenching method was developed to determine nucleic acids (DNA) with the 9-anthracenecarboxylic acid (ACA)-cetyl trimethyl-ammonium bromide (CTAB) system. The fluorescence intensity of ACA was decreased by the addition (CTAB). However, the fluorescence intensity of the system increased dramatically when DNA was added to the solution. The fluorescence enhancement is probably based on the DNA interaction with CTAB. Under the optimum conditions, the changes of fluorescence intensity in the absence and presence of nucleic acids was proportional to the concentration of nucleic acids over the range 0.08-1.0 microg mL(-1) for CT (calf thymus) DNA or FS (fish sperm) DNA. Its detection limits are 0.02 microg mL(-1) for CT DNA and 0.019 microg mL(-1) for FS DNA. Based on this approach, a new quantitative method for DNA assay is presented in this paper.
Liu
Jinshui
J
College of Chemistry and Material Science, Anhui Normal University, Wuhu 241000, PR China. jsliu@sina.com.cn
Wang
Xin
X
Wang
Lun
L
D000818:Animals; D000873:Anthracenes / Q000737:chemistry*; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D005399:Fishes; D005453:Fluorescence; D008297:Male; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013094:Spermatozoa / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry
D000873:Anthracenes; D002593:Cetrimonium Compounds; C010030:9-anthroic acid; D004247:DNA; D000077286:Cetrimonium