Ethanol Perturbs Glycosylation and Inhibits Hypersecretion in Trichoderma reesei.
The effects of ethanol and phenylethanol on the growth of and glycoprotein secretion by Trichoderma reesei were studied. Low levels (1.5%, vol/vol) of ethanol perturbed the glycosylation process, as shown by alterations in the isoelectric profile of the secreted proteins and a reduction in the rate of incorporation of mannose into oligosaccharides. In addition to these effects on posttranslational modification, ethanol drastically lowered the protein secretion level of a hypersecretory strain.
Merivuori
H
H
Department of Biology, Lehigh University, Bethlehem, Pennsylvania 18015.
Montenecourt
B S
BS
Sands
J A
JA