title: Evaluation of endocrine disrupting activity of plasticizers in polyvinyl chloride tubes by estrogen receptor alpha binding assay.
abstract: Polyvinyl chloride (PVC) tubing is an indispensable medical material for extracorporeal circulation therapy. However, di(2-ethylhexyl)phthalate (DEHP), a suspected endocrine disruptor, can be eluted from PVC, suggesting that an alternative material that does not contain DEHP is needed for clinical applications. First, we evaluated the endocrine disrupting risks of the plasticizers contained in PVC tubes by investigating their binding affinities for the human estrogen receptor alpha (ERalpha). Our results revealed that, while DEHP has some binding affinity for ERalpha, neither epoxidized soybean oil nor tris(2-ethylhexyl)trimellitate (an alternative to DEHP) has any affinity for ERalpha. Second, we evaluated the endocrine disrupting risks of a tube made of newly developed plasticizer-free (PF) materials. We confirmed the presence of DEHP and detected several unidentified substances in plasma stored within the PVC tube. This plasma's competitive binding affinity for ERalpha was significantly higher than that of control plasma (P < 0.01). In contrast, the profile of plasma stored in the PF tube was similar to that of the control, both in terms of high-performance liquid chromatography chromatograms and competitive binding capacity for ERalpha, suggesting that the PF tube is biocompatible and is useful for reducing the elution of substances capable of binding to ERalpha.
authors.affiliation: Department of Clinical Biochemistry, Fujita Health University College, Toyoake, Aichi 470-1192, Japan. aohashi@fujita-hu.ac.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ohashi
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Kotera
authors.forename: Hideo
authors.initials: H
authors.lastname: Hori
authors.forename: Makoto
authors.initials: M
authors.lastname: Hibiya
authors.forename: Koji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Murakami
authors.forename: Midori
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Tomita
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Hiki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sugiyama
mesh_terms: D002851:Chromatography, High Pressure Liquid; D020099:Coated Materials, Biocompatible; D004051:Diethylhexyl Phthalate / Q000009:adverse effects / Q000494:pharmacology*; D004703:Endocrine System / Q000187:drug effects*; D047628:Estrogen Receptor alpha / Q000187:drug effects* / Q000378:metabolism; D005112:Extracorporeal Circulation / Q000295:instrumentation*; D006801:Humans; D066298:In Vitro Techniques; D010968:Plasticizers / Q000009:adverse effects / Q000494:pharmacology*; D011143:Polyvinyl Chloride
chemical_list: D020099:Coated Materials, Biocompatible; D047628:Estrogen Receptor alpha; D010968:Plasticizers; D011143:Polyvinyl Chloride; D004051:Diethylhexyl Phthalate