Bioactivity assay of extracts from Calocedrus macrolepis var. formosana bark.
Alcoholic extracts from bark of Calocedrus macrolepis var. formosana Florin (Cupressaceae) were extracted successively using n-hexane, dichloromethane, ethyl acetate, 1-butanol and water, which gave 34.8%, 34.1%, 24.1%, 3.3% and 3.7% soluble fractions, respectively. Antioxidation activity of these fractions by DPPH assay and dissimilar IC50 values of the DPPH showed that ethyl acetate fraction had the best antioxidant activity; its IC50 was 2.6 microg/ml. Analyses of the composition and anti-inflammatory activity of the subfractions from n-C6H14 fraction showed that the T3 and H5ppt had the best anti-inflammatory activity in LPS-stimulated murine macrophage J774A. 1 cells, respectively; moreover, their major constituent was sugiol (T3 37.1%, H5ppt 81.1%), which at dosages of 10 microg/ml inhibited proIL-1beta protein production completely. Furthermore, the T1 also exhibited anti-inflammatory activity, and its major constituent was ferruginol (above 85.6%).
Chao
Louis Kuoping
LK
Department of Biological Science and Technology, Chung Hwa College of Medical Technology, Tainan, Taiwan.
Hua
Kuo-Feng
KF
Hsu
Hsien-Yeh
HY
Su
Yu-Chang
YC
Chang
Shang-Tzen
ST
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D000975:Antioxidants / Q000032:analysis*; D002460:Cell Line; D029779:Cupressaceae / Q000737:chemistry*; D051379:Mice; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts