Effect of radiocontrasts on selected membrane transport systems.
BACKGROUND
This study was aimed at examining the effects of radiocontrast agents on selected membrane transport functions.


METHODS
The effect of diatrizoate sodium (DS), diatrizoate meglumine (DM), and diatrizoate compound (DC) on Na+/K+ pump activity and number, L-arginine, and choline transports were evaluated in erythrocytes of normal individuals and patients undergoing cardiac catheterization.


RESULTS
Norrmal individuals' erythrocytes potassium influxes were 1.50+/-0.35, 1.32+/-0.37, 1.28+/-0.30, and 1.01+/-0.25 mmol/L cell/h in control, DS, DM, and DC groups, respectively (P=0.004; DC vs. control). Patients exposure to Hypaque M-76 significantly diminished Na+/K+ pump activity (1.40+/-0.36 before, vs. 1.27+/-0.40 mmol/L cell/h after; P=0.039). The number of Na+/K+ pumps was reduced (156+/-36 vs. 143+/-34 pumps/erythrocyte; P=0.015) in presence of DS. L-arginine and choline transports changed only at high radiocontrast concentrations.


CONCLUSIONS
Selective changes in erythrocytes membrane transport function take place on exposure to radiocontrasts.
de Oliveira
Rubens Marona
RM
Programa de Pós-Graduação em Medicina e Ciências da Saúde (Nefrologia), Faculdade de Medicina, Hospital São Lucas e Instituto de Pesquisas Biológicas da Pontifícia, Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil. marona@pucrs.br
Pinheiro da Costa
Bartira Ercília
BE
Fervenza
Fernando Custódio
FC
Wagner
Mário Bernardes
MB
d'Avila
Domingos Otavio
DO
Poli de Figueiredo
Carlos Eduardo
CE
D000704:Analysis of Variance; D001120:Arginine / Q000187:drug effects / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D006328:Cardiac Catheterization; D016022:Case-Control Studies; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D011336:Probability; D019275:Radiopharmaceuticals / Q000009:adverse effects*; D012016:Reference Values; D012680:Sensitivity and Specificity; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects* / Q000502:physiology
D019275:Radiopharmaceuticals; D001120:Arginine; D000254:Sodium-Potassium-Exchanging ATPase