title: Induction and Purification of Endo-beta-N-Acetylglucosaminidase from Arthrobacter protophormiae Grown in Ovalbumin.
abstract: Arthrobacter protophormiae produced a high level of extracellular endo-beta-N-acetylglucosaminidase when cells were grown in a medium containing ovalbumin. The enzyme was induced by the glycopeptide fraction of ovalbumin prepared by pronase digestion. Production of the enzyme was also induced by glycoproteins such as yeast invertase and bovine ribonuclease B but not by monosaccharides such as mannose, N-acetylglucosamine, and galactose. The enzyme was purified to homogeneity as demonstrated by polyacrylamide gel electrophoresis and has an apparent molecular weight of about 80,000. The enzyme showed a broad optimum pH in the range of pH 5.0 to 11.0. The enzyme hydrolyzed all heterogeneous ovalbumin glycopeptides, although the hydrolysis rates for hybrid type glycopeptides were very low. The substrate specificity of A. protophormiae endo-beta-N-acetylglucosaminidase was very similar to that of Endo-C(II) from Clostridium perfringens. Therefore, the enzyme induction by A. protophormiae seems to have a close relation to the substrate specificity of the enzyme.
authors.affiliation: Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-07, and Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takegawa
authors.forename: M
authors.initials: M
authors.lastname: Nakoshi
authors.forename: S
authors.initials: S
authors.lastname: Iwahara
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Tochikura