Cloning of Protocatechuate 3,4-Dioxygenase Genes from Bradyrhizobium japonicum USDA110.
A heterologous gene probe encoding the alpha and beta subunits of the Pseudomonas cepacia protocatechuate 3,4-dioxygenase (PCD) was used to detect its homolog in the genome of Bradyrhizobium japonicum USDA110. Three cosmid clones carrying a 2.2-kb BamHI insert showed high levels of PCD activity. SacI digestion of one of the genomic clones, pBjG17, produced a 2.5-kb insert DNA that complemented a PCD mutant of P. cepacia.
Podila
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GK
Department of Biological Sciences, Michigan Technological University, Houghton, Michigan 49931, and Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Hyattsville, Maryland 20782.
Kotagiri
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Shantharam
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