title: Primate-specific endogenous cis-antisense transcription in the human 5q31 protocadherin gene cluster.
abstract: Protocadherins (PCDH), localized to synaptic junctions, contribute to the formation of neuronal networks during brain development; thus, it is speculated that protocadherins may play a role in evolution of neuronal complexity. While protocadherin genes are highly conserved in vertebrates, EST evidence from the locus suggests apparently species-specific cis-antisense transcripts. Novel cis-antisense transcripts, which partially overlap the PCDHalpha12 variable exon, PCDHbeta3 single-exon gene, and PCDHpsi5 unprocessed pseudogene in the human 5q31 PCDHalpha/beta/gamma gene cluster and which are coexpressed with sense-strand transcripts in fetal and adult brain, were identified computationally and validated by gene-specific strand-specific reverse transcriptase PCR (SSRTPCR) and sequencing. Absence of antisense transcripts arising from equivalent genomic locations in mouse indicates that the antisense transcripts originated in the primates after the primate-rodent divergence. Furthermore, not all expected orthologues of human sense and antisense PCDH transcripts were detected in rhesus macaque brain, implying that protocadherin expression patterns differ between primate species. RT followed by quantitative real-time PCR (QPCR) analysis of the three genes in the brain of all three species, and of the PCDHbeta15 gene paralogous to PCDHpsi5 in human and rhesus, revealed that the presence of antisense transcripts was significantly associated with lower sense expression levels across all orthologues. This inverse relationship, along with the pattern of sense and antisense coexpression in the brain, is consistent with a regulatory role for the primate-specific PCDH cis-antisense transcripts, which may represent recent evolutionary inventions modulating the activity of this conserved gene cluster.
authors.affiliation: Genome Institute of Singapore, 60 Biopolis Street #02-01, Singapore 138672, Singapore. LL@gis.a-star.edu.sg
authors.forename: Leonard
authors.initials: L
authors.lastname: Lipovich
authors.forename: Ravi Raj
authors.initials: RR
authors.lastname: Vanisri
authors.forename: Say Li
authors.initials: SL
authors.lastname: Kong
authors.forename: Chin-Yo
authors.initials: CY
authors.lastname: Lin
authors.forename: Edison T
authors.initials: ET
authors.lastname: Liu
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics*; D002895:Chromosomes, Human, Pair 5; D016373:DNA, Antisense / Q000502:physiology*; D006801:Humans; D005810:Multigene Family; D011323:Primates; D011498:Protein Precursors / Q000235:genetics*; D050436:Regulatory Elements, Transcriptional; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D015820:Cadherins; D016373:DNA, Antisense; D011498:Protein Precursors