title: Immobilization of horseradish peroxidase on multi-wall carbon nanotubes and its electrochemical properties.
abstract: Horseradish peroxidase (HRP) was immobilized on carboxylated multi-wall carbon nanotubes in the presence of a coupling reagent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. The immobilized HRP maintained its oxidative activity for guaiacol over a broad range of pH values (4-9). An electrode of graphite rod, 6 mm diam. was fabricated using the immobilized HRP. Cyclic voltammetry of the enzyme electrode confirmed electron transfer between the immobilized HRP and the electrode in the presence of H2O2 but without an added mediator or a reducing substrate.
authors.affiliation: School of Chemical Engineering and Bioengineering, University of Ulsan, 680-749, Ulsan, Korea.
authors.forename: Yoon-Mee
authors.initials: YM
authors.lastname: Lee
authors.forename: O-Yul
authors.initials: OY
authors.lastname: Kwon
authors.forename: Yeo-Joon
authors.initials: YJ
authors.lastname: Yoon
authors.forename: Keungarp
authors.initials: K
authors.lastname: Ryu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D001709:Biotechnology / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D006139:Guaiacol / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D006139:Guaiacol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D005022:Ethyldimethylaminopropyl Carbodiimide