title: Isolation, Purification, and Some Properties of Penicillium chrysogenum Tannase.
abstract: Tannase isolated from Penicillium chrysogenum was purified 24-fold with 18.5% recovery after ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. Optimum enzyme activity was recorded at pH 5.0 to 6.0 and at 30 to 40 degrees C. The enzyme was stable up to 30 degrees C and within the pH range of 4.0 to 6.5. The K(m) value was found to be 0.48 x 10 M when tannic acid was used as the substrate. Metal salts at 20 mM inhibited the enzyme to different levels.
authors.affiliation: Bacteriology Laboratory, Central Leather Research Institute, Madras-600 020, India.
authors.forename: G S
authors.initials: GS
authors.lastname: Rajakumar
authors.forename: S C
authors.initials: SC
authors.lastname: Nandy