Enrichment of Auxotrophic Mutants in Streptomyces griseus.
A method for the isolation and enrichment of tryptophan auxotrophic mutants of the indolmycin-producing strain Streptomyces griseus ATCC 12648 was developed, using penicillin selection. With UV irradiation of 2.2 x 10 muW cm, a mutation rate to tryptophan auxotrophy of 5.6 x 10 was achieved. With 300 mug ml of penicillin G, an enrichment of tryptophan auxotrophs of about 1,000-fold was attained. Approximately 40% of all survivors were tryptophan auxotrophs.
Werner
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RG
Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
Demain
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AL