Identification of the Vibrio vulnificus wbpP gene and evaluation of its role in virulence.
A wbpP gene encoding a putative UDP-N-acetyl-D-glucosamine C(4) epimerase was identified and cloned from Vibrio vulnificus. The functions of the wbpP gene, assessed by the construction of an isogenic mutant and by evaluating its phenotype changes, demonstrated that WbpP is essential in both the pathogenesis and the capsular polysaccharide biosynthesis of V. vulnificus.
Park
Na Young
NY
Department of Food Science and Technology, School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, South Korea.
Lee
Jeong Hyun
JH
Kim
Myung Won
MW
Jeong
Hee Gon
HG
Lee
Byung Cheol
BC
Kim
Tae Sung
TS
Choi
Sang Ho
SH
D000818:Animals; D016667:Bacterial Capsules / Q000096:biosynthesis / Q000235:genetics; D002238:Carbohydrate Epimerases / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014735:Vibrio Infections / Q000201:enzymology / Q000473:pathology; D041261:Vibrio vulnificus / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D002238:Carbohydrate Epimerases; C023223:UDP-N-acetylglucosamine 4-epimerase