High-performance SPME/AP MALDI system for high-throughput sampling and determination of peptides.
This paper presents the performance characteristics for a new multiplexed solid-phase microextraction/atmospheric pressure matrix-assisted laser desorption/ionization (SPME/AP MALDI) source configuration for a hybrid quadrupole-linear ion trap instrument. The results demonstrate that thorough optimization of parameters such as SPME coating material, optics configurations, extraction solvents, and fiber capacity provides dramatic sensitivity improvements (>1000x) over previous reports in the literature. The multiplexed SPME plate is capable of simultaneous extraction from 16 different wells on a multiwell plate, eliminating the need for extensive sample preparation. Subfemtomole sensitivity is demonstrated for peptide standards and protein digests with run-run reproducibility ranging from approximately 13 to 31%. This high-performance SPME/AP MALDI system shows potential for high-throughput extraction from biological samples.
Wang
Yan
Y
Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada, and MDS SCIEX, Concord, Ontario L4K 4V8, Canada.
Schneider
Bradley B
BB
Covey
Thomas R
TR
Pawliszyn
Janusz
J
D000803:Angiotensin I / Q000302:isolation & purification; D000804:Angiotensin II / Q000302:isolation & purification; D001920:Bradykinin / Q000302:isolation & purification; D010455:Peptides / Q000032:analysis*; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
D010455:Peptides; D011108:Polymers; D011758:Pyrroles; D000804:Angiotensin II; C067635:polypyrrole; D000803:Angiotensin I; D001920:Bradykinin