title: Comparative proteomic analysis of B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis.
abstract: OBJECTIVE
  To compare the protein difference between B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis.
  
  
  METHODS
  Protein samples were prepared by vorterx, ultrasonic treatment, and centrifugation. Protein concentrations were determined by Bradford method. Protein difference was compared by the first IEF and the second SDS-polyacrylamide gel electrophoresis.
  
  
  RESULTS
  Protein concentrations in samples of Bartonella henselae Houston and Bartonella henselae Marseille were 2.117 microg/microL and 2.200 microg/microL respectively. Sample protein of 40 microg for IPG strips loading was perfect. The results of 2-DE in pH 4 to 7 IPG strips showed that the total protein spots of Bartonella henselae Houston and Bartonella henselae Marseille were 375 and 379 respectively, 95% of the spots were the same between the two strains of Bartonella henselae.
  
  
  CONCLUSIONS
  The procedure of 2-DE may prove successful for the proteomic analysis of Bartonella henselae. Bartonella henselae Houston and Bartonella henselae Marseille are different genotypes.
authors.affiliation: Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France. suqingz@hotmail.com
authors.forename: Su-Qing
authors.initials: SQ
authors.lastname: Zhao
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Cai
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Zhu
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D018416:Bartonella henselae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005838:Genotype; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins