title: New Arabidopsis recombinant inbred line populations genotyped using SNPWave and their use for mapping flowering-time quantitative trait loci.
abstract: The SNPWave marker system, based on SNPs between the reference accessions Colombia-0 and Landsberg erecta (Ler), was used to distinguish a set of 92 Arabidopsis accessions from various parts of the world. In addition, we used these markers to genotype three new recombinant inbred line populations for Arabidopsis, having Ler as a common parent that was crossed with the accessions Antwerp-1, Kashmir-2, and Kondara. The benefit of using multiple populations that contain many similar markers and the fact that all markers are linked to the physical map of Arabidopsis facilitates the quantitative comparison of maps. Flowering-time variation was analyzed in the three recombinant inbred line populations. Per population, four to eight quantitative trait loci (QTL) were detected. The comparison of the QTL positions related to the physical map allowed the estimate of 12 different QTL segregating for flowering time for which Ler has an allele different from one, two, or three of the other accessions.
authors.affiliation: Laboratory of Genetics, Wageningen University, The Netherlands.
authors.forename: Mohamed E
authors.initials: ME
authors.lastname: el-Lithy
authors.forename: Leónie
authors.initials: L
authors.lastname: Bentsink
authors.forename: Corrie J
authors.initials: CJ
authors.lastname: Hanhart
authors.forename: Gerda J
authors.initials: GJ
authors.lastname: Ruys
authors.forename: Daniela
authors.initials: D
authors.lastname: Rovito
authors.forename: José L M
authors.initials: JL
authors.lastname: Broekhof
authors.forename: Hein J A
authors.initials: HJ
authors.lastname: van der Poel
authors.forename: Michiel J T
authors.initials: MJ
authors.lastname: van Eijk
authors.forename: Dick
authors.initials: D
authors.lastname: Vreugdenhil
authors.forename: Maarten
authors.initials: M
authors.lastname: Koornneef
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D035264:Flowers / Q000235:genetics* / Q000502:physiology; D008040:Genetic Linkage; D005819:Genetic Markers; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D011995:Recombination, Genetic
chemical_list: D005819:Genetic Markers