title: In vitro efficacy of lufenuron against filamentous fungi and blood concentrations after PO administration in horses.
abstract: Lufenuron is a benzoylphenyl urea-derived insecticide that has been recently introduced as a novel treatment for fungal infections in horses. The purposes of this study were to determine (1) the in vitro efficacy of lufenuron against Aspergillus and Fusarium spp. and (2) the ability of lufenuron to reach efficacious blood concentrations after PO administration in horses. Fungal colonies isolated from diseased equine corneas were tested against lufenuron solutions up to 700 microg/mL. Twenty-one adult horses received 1 of 3 PO lufenuron treatment regimens: 5 mg/kg body weight (BW) q24h for 3 days, 20 mg/kg BW q24h for 3 days, or 60 mg/ kg BW q24h for 1 day. Blood samples were collected up to 96 hours after drug administration and analyzed by high-performance liquid chromatography. Statistical analyses of lufenuron blood concentrations were performed by analysis of variance and Fischer's Least Significant Difference test, with statistical significance set at P < .05. Lufenuron showed no effect on the in vitro growth of Aspergillus or Fusarium spp. Lufenuron was detected in the blood of all but 1 horse and showed no adverse effects. The maximum blood lufenuron concentration (83.5 +/- 58.7 microg/L) was lower than the concentrations proven to be ineffective in vitro in this study. Further therapeutic use of lufenuron as an antifungal agent in horses should be based on proven efficacy against specific strains of clinically relevant fungi with pharmacokinetic data demonstrating sufficient lufenuron concentrations in target tissues.
authors.affiliation: Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA. scottyn@mail.vetmed.ufl.edu
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Scotty
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Evans
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Giuliano
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Johnson
authors.forename: George E
authors.initials: GE
authors.lastname: Rottinghaus
authors.forename: Annette W
authors.initials: AW
authors.lastname: Fothergill
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Cutler
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001230:Aspergillus / Q000187:drug effects*; D001549:Benzamides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics / Q000494:pharmacology*; D005260:Female; D005670:Fusarium / Q000187:drug effects*; D006736:Horses / Q000382:microbiology*; D007306:Insecticides / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics / Q000494:pharmacology*; D008297:Male; D005466:Fluorocarbons
chemical_list: D001549:Benzamides; D007306:Insecticides; C070364:fluphenacur; D005466:Fluorocarbons