Determination of some polycyclic aromatic hydrocarbons in filter tar of Turkish cigarettes.
A simultaneous determination method for the analysis of benzo[a]pyrene (BaP) and dibenzo[a,h]anthracene (DahA) in the filter tar of Turkish cigarettes has been developed. The method involved (a) the extraction of BaP and DahA with n-hexane from ACN solution in which the cigarette filters were extracted, and then (b) purification of the n-hexane extracts by elution on an XAD-2 column using n-hexane/dichloromethane (9:1, v/v) mixture. Separation and quantitative determination of BaP and DahA in the extracts were carried out by HPLC and fluorescence detection on a C18 RP column. The calculated recoveries for BaP and DahA were found in the range of 90-100% for each extraction and clean-up steps. Analysis of various filter tar of Turkish cigarettes showed that an average of 74.28 ng/filter of BaP and 5.24 ng/filter of DahA were present in Turkish cigarettes.
Aygün
Seker Fatma
SF
Department of Chemistry, Sciences and Arts Faculty, Ondokuz Mayis University, Samsun, Turkey. faygun@omu.edu.tr
D001551:Benz(a)Anthracenes / Q000032:analysis; D001564:Benzo(a)pyrene / Q000032:analysis; D002273:Carcinogens / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D013638:Tars / Q000737:chemistry*; D014421:Turkey
D001551:Benz(a)Anthracenes; D002273:Carcinogens; D011084:Polycyclic Aromatic Hydrocarbons; D013638:Tars; C024746:tobacco tar; D001564:Benzo(a)pyrene; C026486:1,2,5,6-dibenzanthracene