title: Construction of killer wine yeast strain.
abstract: A double-stranded RNA plasmid which confers the superkiller phenotype was transferred into a wine yeast (Montrachet strain 522) and its leucine-requiring derivative (strain 694) by cytoduction, using the protoplast fusion technique. The killer wine yeast constructed completely suppressed the growth of killer-sensitive strains of Saccharomyces cerevisiae in yeast extract-peptone-glucose medium at pH 4.5, whereas the killer effect was somewhat decreased at pH 3.5. The wine yeast harboring the killer factor also inhibited the growth of killer-sensitive cells satisfactorily when it was grown in grape juice.
authors.affiliation: University of California, Davis, California 95616.
authors.forename: T
authors.initials: T
authors.lastname: Seki
authors.forename: E H
authors.initials: EH
authors.lastname: Choi
authors.forename: D
authors.initials: D
authors.lastname: Ryu