title: CTX-M-1 extended-spectrum beta-lactamase-producing Proteus mirabilis in Greece.
abstract: To examine the dissemination of CTX-M-type extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Proteus mirabilis, 91 nonrepetitive ESBL-producing P. mirabilis were collected from infected patients in a tertiary Greek hospital during September, 2001, to May, 2004. A bla (CTX-M) gene was amplified in one isolate (strain A328), but bla (CTX-M) was not detected in any of the remaining ESBL producers. Sequencing results showed that P. mirabilis A328 produced a CTX-M-1 enzyme while PCR mapping of the genetic element carrying bla (CTX-M-1) revealed that the gene was located downstream of an ISEcp1B element. The cefotaxime resistance determinant was easily transferable and carried on a 70-kb plasmid. The emergence of CTX-M-1- producing P. mirabilis indicates the need for early recognition of such strains to be able to control their spread in our hospital and community environment.
authors.affiliation: Department of Microbiology, Hippokration University Hospital, 546 42 Thessaloniki, Greece.
authors.forename: Paraskevi
authors.initials: P
authors.lastname: Karapavlidou
authors.forename: Danai
authors.initials: D
authors.lastname: Sofianou
authors.forename: Evangelos N
authors.initials: EN
authors.lastname: Manolis
authors.forename: Spyros
authors.initials: S
authors.lastname: Pournaras
authors.forename: Athanassios
authors.initials: A
authors.lastname: Tsakris
mesh_terms: D006115:Greece; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011513:Proteus mirabilis / Q000201:enzymology* / Q000302:isolation & purification; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C055184:beta-lactamase TEM-3; D001618:beta-Lactamases