title: Clinical benefit of metaphase I oocytes.
abstract: BACKGROUND
  We studied the benefit of using in vitro matured metaphase I (MI) oocytes for ICSI in patients with a maximum of 6 mature metaphase II (MII) oocytes at retrieval.
  
  
  METHODS
  In 2004, 187 ICSI cycles were selected in which maximum 6 MII oocytes and at least one MI oocyte were retrieved. MI oocytes were put in culture to mature until the moment of ICSI, which was performed between 2 to 11 hours after oocyte retrieval (day 0). In exceptional cases, when the patient did not have any mature oocyte at the scheduled time of ICSI, MI oocytes were left to mature overnight and were injected between 19 to 26 hours after retrieval (day 1). Embryos from MI oocytes were chosen for transfer only when no other good quality embryos from MII oocytes were available. Outcome parameters were time period of in vitro maturation (IVM), IVM and fertilization rates, embryo development, clinical pregnancy rates, implantation rates and total MI oocyte utilization rate.
  
  
  RESULTS
  The overall IVM rate was 43%. IVM oocytes had lower fertilization rates compared to in vivo matured sibling oocytes (52% versus 68%, P < 0.05). The proportion of poor quality embryos was significantly higher in IVM derived oocytes. One pregnancy and live birth was obtained out of 13 transfers of embryos exclusively derived from IVM oocytes. This baby originated from an oocyte that was injected after 22 hrs of IVM.
  
  
  CONCLUSIONS
  Fertilization of in vitro matured MI oocytes can result in normal embryos and pregnancy, making IVM worthwhile, particularly when few MII oocytes are obtained at retrieval.
authors.affiliation: Infertility Centre, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium. leen.vanhoutte@UGent.be
authors.forename: Leen
authors.initials: L
authors.lastname: Vanhoutte
authors.forename: Petra
authors.initials: P
authors.lastname: De Sutter
authors.forename: Josiane
authors.initials: J
authors.lastname: Van der Elst
authors.forename: Marc
authors.initials: M
authors.lastname: Dhont
mesh_terms: D018929:Cell Culture Techniques; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007246:Infertility / Q000628:therapy; D008297:Male; D008677:Metaphase; D009865:Oocytes / Q000166:cytology*; D011247:Pregnancy; D018873:Pregnancy Rate; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods / Q000592:standards; D013997:Time Factors