title: Hollow-fiber blood-dialysis membranes: superoxide generation, permeation, and dismutation measured by chemiluminescence.
abstract: The interaction of blood with a material surface results in activation of the body's humoral immune system and the generation of reactive oxygen species (ROS). It has recently become clear that ROS are central to the pathology of many diseases. In this study, we evaluated the superoxide generation, permeation, and dismutation in hollow-fiber dialysis membranes by using 2-methyl-6-p-methoxyphenylethynyl-imidazopyrazinone (MPEC) as a superoxide-reactive chemiluminescence producer and an optical fiber probe to detect the resulting chemiluminescence in the hollow fiber lumen. We measured the superoxide generated when bovine blood leukocytes were brought into contact with dialysis membranes. Superoxide permeation was determined by measuring MPEC chemiluminescence in the hollow fiber lumen using an optical fiber probe. Additionally, superoxide dismutation was evaluated by examining the difference in superoxide permeability for membranes with and without vitamin E coating. Superoxide generation varies for different membrane materials, depending on the membrane's biocompatibility. Superoxide permeability depends on the diffusive permeability of membranes. No marked decrease in superoxide permeability was observed among membrane materials. The superoxide permeability of vitamin E-coated membrane was smaller than that of uncoated membrane. The antioxidant property of vitamin E-coated membranes is hence effective in causing superoxide dismutation.
authors.affiliation: Department of Chemical Engineering, 21st Century COE Program, Waseda University, 3-4-1 Okubo, Tokyo 169-8555, Japan.
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Kosuke
authors.initials: K
authors.lastname: Endo
authors.forename: Takehiro
authors.initials: T
authors.lastname: Miyasaka
authors.forename: Seiichi
authors.initials: S
authors.lastname: Mochizuki
authors.forename: Fukashi
authors.initials: F
authors.lastname: Kohori
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Sakai
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002417:Cattle; D020099:Coated Materials, Biocompatible; D016166:Free Radical Scavengers / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D049449:Luminescence; D008163:Luminescent Measurements; D008567:Membranes, Artificial; D010539:Permeability; D006435:Renal Dialysis / Q000295:instrumentation*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D020099:Coated Materials, Biocompatible; D016166:Free Radical Scavengers; D008567:Membranes, Artificial; D013481:Superoxides; D014810:Vitamin E; D013482:Superoxide Dismutase