title: A novel ATP/ADP hydrolysis activity of hyperthermostable group II chaperonin in the presence of cobalt or manganese ion.
abstract: A novel ATPase activity that was strongly activated in the presence of either cobalt or manganese ion was discovered in the chaperonin from hyperthermophilic Pyrococcus furiosus (Pfu-cpn). Surprisingly, a significant ADPase activity was also detected under the same conditions. A more extensive search revealed similar nucleotide hydrolysis activities in other thermostable chaperonins. Chaperonin activity, i.e., thermal stabilization and refolding of malate dehydrogenase from the guanidine-hydrochloride unfolded state were also detected for Pfu-cpn under the same conditions. We propose that the novel cobalt/manganese-dependent ATP/ADPase activity may be a common trait of various thermostable chaperonins.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Tottori University, Tottori 680-8552, Japan.
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Hongo
authors.forename: Hidenori
authors.initials: H
authors.lastname: Hirai
authors.forename: Chisato
authors.initials: C
authors.lastname: Uemura
authors.forename: Shujiro
authors.initials: S
authors.lastname: Ono
authors.forename: Junjiro
authors.initials: J
authors.lastname: Tsunemi
authors.forename: Takashi
authors.initials: T
authors.lastname: Higurashi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Mizobata
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawata
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018833:Chaperonins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D003035:Cobalt / Q000378:metabolism* / Q000494:pharmacology; D006358:Hot Temperature; D008291:Malate Dehydrogenase / Q000737:chemistry; D008345:Manganese / Q000378:metabolism* / Q000494:pharmacology; D017510:Protein Folding; D019711:Pyrococcus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics
chemical_list: D019843:Archaeal Proteins; D003035:Cobalt; D008345:Manganese; D008291:Malate Dehydrogenase; D000251:Adenosine Triphosphatases; D018833:Chaperonins