title: [Photochemical activity of photosystem II and hydrogen photoproduction in sulfur-deprived Chlamydomonas reinhardtii mutants D1-R323D and D1-R323L].
abstract: The role of photosystem II in hydrogen photoproduction by Chlamydomonas reinhardtii cells was studied in mutants with modified D1-protein. In D1-R323D and D1-R323L mutants, the replacement of arginine by aspartate or leucine, respectively, resulted in the disruption of electron transport at the donor side of photosystem II. The rate of oxygen evolution in D1-R323D decreased twice as compared to the pseudo-wild type (pWT), and in D1-R323L no oxygen evolution was detected. The latter mutant was not capable of photoautotrophical growth. The dynamics of changes in oxygen content, the reduction of photosystem II active reaction centers (deltaF/F(1)m), and hydrogen production rate in pWT were found to be similar to the wild type if cultivated under sulfur deprivation in a closed bioreactor. The observed gradual decrease in the deltaF/F(1)m value turned to a sharp drop almost to zero followed by a partial recovery during which the production of hydrogen set in. The transition to the anaerobic phase in D1-R323D cultured in a sulfur-deprived medium occurred earlier than it happened in pWt under the same conditions. However, the partial recovery of photosystem II activity and hydrogen production started at a later time, and the rate of hydrogen production was low. The D1-R323L mutant incapable of oxygen evolution entered the rapidly anaerobiosis but produced no hydrogen. The kinetics of photoinduced redox transitions in P700 was similar in all investigated strains and was not affected by diuron addition. This implies that the mutants had a pool of reducers, which could donate electrons through the quinone pool or cytochrome to photosystem I. However, in D1-R323L mutant lacking the active photosystem II, this condition was not sufficient to support hydrogenase activity.
authors.forename: V V
authors.initials: VV
authors.lastname: Makarova
authors.forename: S N
authors.initials: SN
authors.lastname: Kosourov
authors.forename: T E
authors.initials: TE
authors.lastname: Krendeleva
authors.forename: G P
authors.initials: GP
authors.lastname: Kukarskikh
authors.forename: M L
authors.initials: ML
authors.lastname: Ghirardi
authors.forename: M
authors.initials: M
authors.lastname: Seibert
authors.forename: A B
authors.initials: AB
authors.lastname: Rubin
mesh_terms: D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism*; D004579:Electron Transport / Q000235:genetics; D006859:Hydrogen / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism*; D017354:Point Mutation; D013455:Sulfur / Q000378:metabolism*
chemical_list: D020418:Algal Proteins; D045332:Photosystem II Protein Complex; D013455:Sulfur; D006859:Hydrogen