Optimization of the cell seeding density and modeling of cell growth and metabolism using the modified Gompertz model for microencapsulated animal cell culture.
Cell microencapsulation is one of the promising strategies for the in vitro production of proteins or in vivo delivery of therapeutic products. In order to design and fabricate the optimized microencapsulated cell system, the Gompertz model was applied and modified to describe the growth and metabolism of microencapsulated cell, including substrate consumption and product formation. The Gompertz model successfully described the cell growth kinetics and the modified Gompertz models fitted the substrate consumption and product formation well. It was demonstrated that the optimal initial cell seeding density was about 4-5 x 10(6) cells/mL of microcapsule, in terms of the maximum specific growth rate, the glucose consumption potential and the product formation potential calculated by the Gompertz and modified Gompertz models. Modeling of cell growth and metabolism in microcapsules provides a guideline for optimizing the culture of microencapsulated cells.
Wen-tao
Qi
Q
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Ying
Zhang
Z
Juan
Ma
M
Xin
Guo
G
Yu-bing
Xie
X
Wei
Wang
W
Xiaojun
Ma
M
D000464:Alginates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D016466:CHO Cells; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D004734:Energy Metabolism / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008567:Membranes, Artificial; D008954:Models, Biological; D011107:Polylysine / Q000031:analogs & derivatives / Q000737:chemistry
D000464:Alginates; D001672:Biocompatible Materials; D008567:Membranes, Artificial; C051546:alginate-polylysine-alginate; D011107:Polylysine; D019344:Lactic Acid; D005947:Glucose