[Determination of fibronectin-binding region of FBPS of Streptococcus suis type 2].
Fusion expression plasmid pFBPS, pFBPS (7 - 82), pFBPS (7 - 165) and pFBPS3 (87 - 320) of Streptococcus suis type strain HA9801 were generated by cloning different fragments of fbps amplified from pMD-T-FBPS by PCR into plasmid pET-32a(+). It has been confirmed that the recombinant proteins, FBPS, FBPS(87 -320), and FBPS(7 - 167), which are expressed by recombinant plasmid pFBPS, pFBPS(87 - 320) and pFBPS(7 - 165), respectively, bound human fibronectin by ligand affinity Western blot assay. The results indicate the primary fibronectin-binding domain of FBPS lies within 87 - 165 amino acid residues region.
Sun
Li-yun
LY
Key Laboratory of Animal Disease Diagnostic and Immunology of Ministry of Agriculture, Nanjing Agricultural University, China.
Fan
Hong-jie
HJ
Lu
Cheng-ping
CP
D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D005353:Fibronectins / Q000378:metabolism*; D016366:Open Reading Frames; D010957:Plasmids; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017011:Streptococcus suis / Q000737:chemistry* / Q000145:classification
D018829:Adhesins, Bacterial; D005353:Fibronectins; D011994:Recombinant Proteins; C078635:fibronectin-binding proteins, bacterial