title: Micropatterning of polystyrene nanoparticles and its bioapplications.
abstract: Micropatterning of biomolecules forms the basis of cell culture, biosensor and microarray technology. Currently, the most widely used techniques are photoresist lithography, soft lithography or using robots which all involve multi-step surface modification directly on a planar substrate. Here we report a method to pattern biomolecules through self-assembling polystyrene nanoparticles in arrayed microwells on a solid surface to form well-ordered patterning, followed by attaching biomolecules to the assembled nanoparticles. The formation of colloidal patterns depends on capillary force, surface wettability and physical confinement. This method can be used for micropatterning a variety of biomolecules such as protein and antibody.
authors.affiliation: Division of Bioengineering, Faculty of Engineering, National University of Singapore.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.forename: Fung Ling
authors.initials: FL
authors.lastname: Yap
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D003102:Colloids / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*; D045663:Quantum Dots; D013499:Surface Properties
chemical_list: D003102:Colloids; D011137:Polystyrenes