Improved method for detection of starch hydrolysis.
A new starch hydrolysis detection method which does not rely on iodine staining or the use of color-complexed starch is described. A linear relationship was obtained with agar-starch plates when net clearing zones around colonies of yeasts were plotted against enzyme levels (semilogarithm scale) produced by the same yeast strains in liquid medium. A similar relationship between starch clearing zones and alpha-amylase levels from three different sources was observed. These observations suggest that the method is useful in mutant isolations, strain improvement programs, and the prediction of alpha-amylase activities in culture filtrates or column effluents.
Dhawale
M R
MR
Agricultural Microbiology Section, Dairy and Food Science Department, University of Saskatchewan, Saskatoon, Canada, S7N 0W0.
Wilson
J J
JJ
Khachatourians
G G
GG
Ingledew
W M
WM