title: Endothelial ephrinB2 is controlled by microenvironmental determinants and associates context-dependently with CD31.
abstract: OBJECTIVE
  The EphB ligand ephrinB2 has been identified as a critical determinant of arterial endothelial differentiation and as a positive regulator of invading endothelial cells during angiogenesis. This study was aimed at identifying determinants of endothelial cell ephrinB2 expression.
  
  
  RESULTS
  Arteriovenous asymmetrical endothelial cell ephrinB2 expression in vivo is lost on transfer into culture with aortic endothelial cells becoming partially ephrinB2-negative and saphenous vein endothelial cells becoming partially ephrinB2-positive. Contact with smooth muscle cells and angiogenic stimulation by vascular endothelial growth factor lead to an increased endothelial cell ephrinB2 expression. Quiescent, smooth muscle-contacting endothelial cells express ephrinB2 uniformly on their luminal surface. In contrast, monolayer endothelial cells translocate ephrinB2 to interendothelial cell junctions, which is strongly enhanced by EphB4-Fc-mediated receptor body activation. Junctional ephrinB2 colocalizes and coimmunoprecipitates with CD31.
  
  
  CONCLUSIONS
  This study identifies distinct regulatory mechanisms of endothelial ephrinB2 expression and cellular distribution in quiescent and activated endothelial cells. The data demonstrate that endothelial cell ephrinB2 expression is controlled by microenvironmental determinants rather than being an intrinsic endothelial cell differentiation marker.
authors.affiliation: Department of Vascular Biology and Angiogenesis Research, Tumor Biology Center, Freiburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Korff
authors.forename: Gudrun
authors.initials: G
authors.lastname: Dandekar
authors.forename: Dennis
authors.initials: D
authors.lastname: Pfaff
authors.forename: Tim
authors.initials: T
authors.lastname: Füller
authors.forename: Winfried
authors.initials: W
authors.lastname: Goettsch
authors.forename: Henning
authors.initials: H
authors.lastname: Morawietz
authors.forename: Florence
authors.initials: F
authors.lastname: Schaffner
authors.forename: Hellmut G
authors.initials: HG
authors.lastname: Augustin
mesh_terms: D001011:Aorta / Q000166:cytology; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D036388:Ephrin-B2 / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007365:Intercellular Junctions / Q000378:metabolism; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D012501:Saphenous Vein / Q000166:cytology; D014469:Umbilical Arteries / Q000166:cytology; D014471:Umbilical Veins / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
chemical_list: D036388:Ephrin-B2; D008024:Ligands; D008565:Membrane Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A