A rapid and quantitative detection system for Streptococcus mutans in saliva using monoclonal antibodies.
A new semiquantitative enumeration system was developed to detect Streptococcus mutans in saliva. Using species-specific monoclonal antibodies, the system quickly detected salivary S. mutans in 30 min and classified the results into three levels. In this study, saliva samples collected from 28 young adults aged between 22 and 24 years were subjected to the monoclonal antibody-based detecting system and selective medium-based detecting methods. The results generated from the PCR-confirmed culture method indicated the mean salivary S. mutans counts at level 1, 2 and 3 were 5.7 x 10(4), 1.3 x 10(5) and 3.4 x 10(6) CFU/ml, respectively. The differences between level 3 and 1 and level 3 and 2 were statistically significant (one-way ANOVA; p < 0.01). The results generated from the system were consistent with the data generated from two culture-based commercial products.
Matsumoto
Y
Y
GC Corporation, Research and Developmental Department, Oral Health Care Group, Tokyo, Japan. yuko_matsumoto@mb.gcdental.co.jp
Sugihara
N
N
Koseki
M
M
Maki
Y
Y
D000328:Adult; D000704:Analysis of Variance; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D015373:Bacterial Typing Techniques; D015169:Colony Count, Microbial; D006801:Humans; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000382:microbiology*; D013295:Streptococcus mutans / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal