title: Identification of human papillomavirus DNA in melanoma biopsy specimens of Greek population.
abstract: The aim of this retrospective study was to evaluate melanoma biopsy specimens from the Greek population living in the prefecture of Larissa for the presence of human papillomavirus (HPV) DNA and to determine the possible relationship between HPV and clinical outcome in these patients. Twenty-eight melanoma biopsy specimens, 20 from primary cutaneous melanoma and eight from melanoma metastasis were obtained from 28 patients. The biopsy samples were formalin-fixed and paraffin wax-embedded. The control group consisted of three junctional melanocytic nevi, histologically confirmed, and three punch biopsies from normal skin that were obtained from six healthy individuals. The presence and types of HPV DNA were assessed by the amplification of a fragment of the LI region by consensus primer polymerase chain reaction (PCR) combined with restriction fragment length polymorphism analysis (RFLPA). In each biopsy specimen that was evaluated, HPV 6, HPV 11, HPV 16 and HPV 18 positive controls from genital HPV lesions were included. Five of 28 (17.85%) biopsy melanoma specimens were positive for HPV DNA. Conversely, HPV was not detected in any of the biopsy specimens of the control group (0/6). HPV viral type 16 was found in two samples and HPV 6 DNA in three. Our results regarding the possible relationship between melanoma and HPV DNA were not statistically significant (p > 0.05). These findings suggest that ultraviolet sun exposure remains the main cause of melanoma in our region. The role of cutaneous HPV infection in the pathogenesis of melanoma remains elusive.
authors.affiliation: Department of Dermatology, University of Thessaly, Larissa, Greece.
authors.forename: A V
authors.initials: AV
authors.lastname: Roussaki-Schulze
authors.forename: C
authors.initials: C
authors.lastname: Kouskoukis
authors.forename: C
authors.initials: C
authors.lastname: Rammos
authors.forename: E
authors.initials: E
authors.lastname: Rallis
authors.forename: F
authors.initials: F
authors.lastname: Kontos
authors.forename: E
authors.initials: E
authors.lastname: Zafiriou
authors.forename: G
authors.initials: G
authors.lastname: Gross
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006115:Greece; D006801:Humans; D008297:Male; D008545:Melanoma / Q000473:pathology / Q000821:virology*; D009362:Neoplasm Metastasis; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000473:pathology / Q000821:virology*
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral