title: [Diagnostic difficulties of Toxoplasma gondii infection in pregnant women. Is it possible to explain doubts by polymerase chain reaction?].
abstract: OBJECTIVE
  Toxoplasma gondii infection during pregnancy is still a difficult problem in the contemporary perinatology. Difficulties met during interpretation of serological tests carried out in pregnant patients to detect Toxoplasmosis implies more and more frequent use of the Polymerase Chain Reaction (PCR).
  
  
  METHODS
  To evaluate the dependence between serological tests and quantity of the Toxoplasma gondii genomes in mothers' blood and amniotic fluid or neonatal blood, the quantitative PCR (q-PCR) method was applied.
  
  
  METHODS
  The analysis was performed in 81 pregnant women. Maternal blood, amniotic fluid and newborns' umbilical blood samples were evaluated for the presence of Toxoplasma gondii DNA. IgG and IgM Toxoplasma gondii antibodies were evaluated by the ELISA method.
  
  
  RESULTS
  High seroprevalence (51.9%) of the Toxoplasma gondii was confirmed. Toxoplasma gondii genetic material in blood and/or amniotic fluid was found in 33 patients. It was stated that quantity of the protozoa and anti-IgM presence in mothers' blood are the factors influencing significantly the Toxoplasma gondii manifestation in amniotic fluid.
  
  
  CONCLUSIONS
  High suitability of PCR in diagnosis of Toxoplasmosis during pregnancy and vertical transmission was confirmed.
authors.affiliation: Klinika Perinatologii i Ginekologii w Zabrzu, SlAM. helen_s@autograf.pl
authors.forename: Helena
authors.initials: H
authors.lastname: Sławska
authors.forename: Bartosz
authors.initials: B
authors.lastname: Czuba
authors.forename: Joanna
authors.initials: J
authors.lastname: Gola
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
authors.forename: Agata
authors.initials: A
authors.lastname: Włoch
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Wilczok
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Kamiński
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000469:parasitology*; D000818:Animals; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology; D011296:Prenatal Diagnosis / Q000379:methods; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014125:Toxoplasmosis, Congenital / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology