Fluorimetric Detection of a Bacillus stearothermophilus Spore-Bound Enzyme, alpha-d-Glucosidase, for Rapid Indication of Flash Sterilization Failure.
A biological indicator based on fluorimetric detection within 60 min of a Bacillus stearothermophilus spore-bound enzyme, alpha-d-glucosidase, has been developed. Results indicate that the enzyme survived slightly longer than spores observed after 24 h of incubation. The new system shows promise for evaluating flash sterilization cycles within 60 min compared with conventional 24-h systems.
Vesley
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School of Public Health, Box 197 Mayo, University of Minnesota, Minneapolis, Minnesota 55455, and 3M Company, St. Paul, Minnesota 55133.
Langholz
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Rohlfing
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Foltz
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