The protons of gluconic acid are the major factor responsible for the dissolution of tricalcium phosphate by Burkholderia cepacia CC-Al74.
Burkholderia cepacia CC-Al74 with a high ability for solubilizing tricalcium phosphate (TCP) was used to study the P-solubilization mechanism. We collected filtrates able to solubilize TCP from the cultures of strain CC-Al74 and demonstrated that the P-solubilization increased from 0 microg ml(-1) to 200 microg ml(-1) during exponential growth, when the pH decreased from 8 to 3. HPLC-analysis revealed that the solubilization of TCP was mainly caused by the release of 16.3 mM gluconic acid. At this concentration, gluconic acid was capable of solubilizing 376 microg ml(-1) of TCP whereas water at pH 3 only solubilized 35 microg ml(-1). The difference is due to the final H+ concentrations which were 13.5 mM and 1 mM in 16.3 mM gluconic acid and deionized water, respectively at pH 3.
Lin
Ta-Fa
TF
Department of Soil and Environmental Sciences, National Chung Hsing University, 250, Kuo-Kaung Road, Taichung, Taiwan 40227, ROC.
Huang
Hsin-I
HI
Shen
Fo-Ting
FT
Young
Chiu-Chung
CC
D016956:Burkholderia cepacia / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002130:Calcium Phosphates / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D005374:Filtration; D005942:Gluconates / Q000032:analysis / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011522:Protons; D012995:Solubility; D014867:Water / Q000737:chemistry
D002130:Calcium Phosphates; D003470:Culture Media; D005942:Gluconates; D011522:Protons; D014867:Water; C018392:tricalcium phosphate; D006851:Hydrochloric Acid; C030691:gluconic acid