Asymmetrically functional surface properties on biocompatible phospholipid polymer membrane for bioartificial kidney.
To obtain a bioartificial kidney composed of a porous polymer membrane and renal cells, a polysulfone (PSf) membrane (PSM) blended with 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer was prepared. The PSM flat membrane with a porous structure could be prepared from the polymer blend containing 1 wt % of the MPC polymer in PSf by the phase inversion technique in a dry-wet process. Asymmetrical surface properties were observed on both sides of the membrane surfaces. That is, the sponge layer formed at the substrate-contacting surface of the membrane had 10-20 microm pores, but the pores in the micrometer range could not be observed for a skin layer formed at the air-contacting surface of the membrane. At the sponge layer surface, the MPC unit composition was 7 times larger than that at the skin layer surface. The amount of proteins adsorbed on the surface corresponded to the MPC unit composition. On the skin layer, a small amount of adsorbed proteins and platelet adhesion could be suppressed compared with those on the sponge layer. However, the skin layer had a moderate protein adsorption, so it showed a sufficient cytocompatibility to enable renal tubule epithelial cells to adhere and proliferate in the membrane. Thus, it functioned well as a renal tubule. Therefore, because of both its hemocompatibility and cytocompatibility, we could conclude that the PSM membrane is useful for as a renal tubule device for a bioartificial kidney.
Ueda
Hideto
H
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Tokyo 113-8656, Japan.
Watanabe
Junji
J
Konno
Tomohiro
T
Takai
Madoka
M
Saito
Akira
A
Ishihara
Kazuhiko
K
D000327:Adsorption; D000818:Animals; D021522:Bioartificial Organs; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D010743:Phospholipids / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D013450:Sulfones / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties; D001613:beta 2-Microglobulin / Q000378:metabolism
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D010743:Phospholipids; D011108:Polymers; D013450:Sulfones; D001613:beta 2-Microglobulin; C017662:polysulfone P 1700