title: Properties of alginate lyases from marine bacteria.
abstract: Alginate lyases (EC 4.2.2.3) from two marine bacteria were isolated and partially characterized. A cell-bound lyase from isolate A3 had a molecular weight of approximately 100,000 and cleaved mannuronate blocks, apparently in an exo manner. A lyase recovered from the culture medium of isolate W3 was soluble in saturated ammonium sulfate, cleaved guluronate blocks, apparently in an endo manner, and had a molecular weight of 35,000. The thiobarbiturate test and urea-polyacrylamide gel electrophoresis were used to determine substrate specificity and mode of substrate cleavage by the enzymes.
authors.affiliation: Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331-2902.
authors.forename: R S
authors.initials: RS
authors.lastname: Doubet
authors.forename: R S
authors.initials: RS
authors.lastname: Quatrano