Immunocytochemical localization of adrenomedullin 2/intermedin-like immunoreactivity in human hypothalamus, heart and kidney.
Adrenomedullin 2/intermedin (AM2/IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CGRP) peptide family. AM2/IMD has a vasodilator action, and antidiuretic and antinatriuretic effects in mice. The aim of the present study is to clarify immunolocalization of AM2/IMD in human hypothalamus, heart and kidney obtained at autopsy. Immunocytochemistry showed AM2/IMD-immunoreactive cell bodies in the paraventricular and supraoptic nuclei of human hypothalamus. Both parvocellular and magnocellular cells in the paravetricular nucleus are immunostained with AM2/IMD. Immunostaining of serial sections showed co-localization of AM2/IMD-like immunoreactivity and vasopressin in the paraventricular nucleus. Myocardial cells of the heart and renal tubular cells were positively immunostained with AM2/IMD, whereas neither renal glomeruli nor vasculature in the heart and kidney were immunostained. Reverse-transcriptase polymerase chain reaction confirmed expression of AM2/IMD mRNA in the brain, pituitary, heart and kidney. The present study has shown the wide expression of AM2/IMD in human hypothalamus, heart and kidney, raising the possibility that this novel peptide may be related to the central and peripheral regulation of the circulation and water-electrolyte metabolism.
Takahashi
Kazuhiro
K
Department of Analytical Medical Technology, Tohoku University School of Health Sciences, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. ktaka-md@mail.tains.tohoku.ac.jp
Kikuchi
Kumi
K
Maruyama
Yutaka
Y
Urabe
Tomoko
T
Nakajima
Kiichiro
K
Sasano
Hironobu
H
Imai
Yutaka
Y
Murakami
Osamu
O
Totsune
Kazuhito
K
D053607:Adrenomedullin; D001921:Brain / Q000378:metabolism; D004573:Electrolytes; D006801:Humans; D007031:Hypothalamus / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods*; D007668:Kidney / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism; D036361:Peptide Hormones / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution; D014867:Water / Q000737:chemistry
C484330:ADM2 protein, human; D004573:Electrolytes; D018377:Neurotransmitter Agents; D036361:Peptide Hormones; D010455:Peptides; D014867:Water; D053607:Adrenomedullin