title: [Standardization of an in-house ELISA for pertussis serology and its application in a seroepidemiological study].
abstract: Seroepidemiological studies need sensitive, practicle and cost-effective methods. For pertussis serosurveillance, an in-house ELISA for antipertussis-toxin (PT) and anti-filamentous-hemagglutinin (FHA) were established in our laboratory and compared by a Ball-ELISA which had been reported to be reliable previously. Sixty sera with various antibody titers were tested by both of the methods. The correlation coefficients between two methods were 0.729 and 0.776 for anti-PT and anti-FHA, respectively, and regression coefficients were 0.623 and 0.693, respectively. The in-house ELISA was applied to a serosurvey including 373 healthy subjects (6 months-91 years old) in Turkey to observe the results. The moving averages of both antibodies were increased until 10 years old, reaching to 31 EU/ml for anti-PT and 65 EU/ml for anti-FHA and kept around this level in the older ages. The in-house ELISA was found to be reliable and the serosurvey results obtained by ELISA showed a characteristic distribution of antibody titers in each age group.
authors.affiliation: Refik Saydam Hifzissihha Merkezi Başkanliği, Salgin Hastaliklar Araştirma Müdürlüğü, Ankara.
authors.forename: Nilay
authors.initials: N
authors.lastname: Cöplü
authors.forename: Berrin
authors.initials: B
authors.lastname: Esen
authors.forename: Demet
authors.initials: D
authors.lastname: Kurtoğlu
authors.forename: Aysegül
authors.initials: A
authors.lastname: Gözalan
authors.forename: Kikuko
authors.initials: K
authors.lastname: Miyamura
authors.forename: Iwao
authors.initials: I
authors.lastname: Yoshida
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood*; D000992:Antitoxins / Q000097:blood; D001886:Bordetella pertussis / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005260:Female; D006388:Hemagglutinins / Q000276:immunology; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D037342:Pertussis Toxin / Q000276:immunology; D016036:Seroepidemiologic Studies; D014421:Turkey / Q000453:epidemiology; D014917:Whooping Cough / Q000453:epidemiology*
chemical_list: D000907:Antibodies, Bacterial; D000992:Antitoxins; D006388:Hemagglutinins; D037342:Pertussis Toxin