Mass spectrometric identification of serine hydrolase OVCA2 in the medulloblastoma cell line DAOY.
OVCA2 is a putative serine-hydrolase. Performing protein profiling in human tumour cell lines, OVCA2 was detected in DAOY medulloblastoma cells as a high abundance protein. The protein was unambiguously identified by 2D gel-electrophoresis and MALDI-MS and MS/MS, its presence was confirmed by western blotting. Immunohistochemistry revealed expression in medulloblastoma and predominantly in oligodendrocytes. Computational approaches predicted functional motifs and domains, interaction with apoptosis-related protein BAG and 3D structure. In addition to the presence of OVCA2 in medulloblastoma, it was furthermore detectable in three out of 10 human tumour cell-lines as a high abundance protein probably suggesting a role in the tumour biology.
Azizi
Amedeo A
AA
Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 19-21, A-1090 Vienna, Austria.
Gelpi
Ellen
E
Yang
Jae-Won
JW
Rupp
Bernhard
B
Godwin
Andrew K
AK
Slater
Carolyn
C
Slavc
Irene
I
Lubec
Gert
G
D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002528:Cerebellar Neoplasms / Q000201:enzymology* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008527:Medulloblastoma / Q000201:enzymology* / Q000473:pathology; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
C456119:OVCA2 protein, human; D011506:Proteins; D012697:Serine Endopeptidases