In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes.
MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.
Kloosterman
Wigard P
WP
Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Wienholds
Erno
E
de Bruijn
Ewart
E
Kauppinen
Sakari
S
Plasterk
Ronald H A
RH
D000818:Animals; D004622:Embryo, Mammalian / Q000737:chemistry*; D004625:Embryo, Nonmammalian / Q000737:chemistry; D020869:Gene Expression Profiling / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D035683:MicroRNAs / Q000032:analysis*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000737:chemistry*; D015027:Zebrafish / Q000235:genetics
D035683:MicroRNAs; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; C477371:locked nucleic acid