[The elp3 subunit of human elongator complex ignificantly complemented the growth defects of yeast elp3delta strain].
The human elongator complex was found to be very similar to the yeast Elongator both in composition and its interaction with RNA polymerase II. But little is known about its functions in vivo. In this study, we analyzed the functions of the help3, the catalytic subunit of human Elongator, using a yeast complementation system. The results indicated that help3 was able to significantly complement the growth defects of the elp3delta yeast strain under high temperature and caffeine conditions. Gene expression analysis showed that help3 significantly resumed the slow activation of the pho5 gene under the low phosphate condition, and when heat shocked it increased the expression level of ssa3 gene. The yhelp3 containing the HAT domain of human elp3 and the remainder of yeast elp3 had a higher complementation ability than human elp3, while the yhelp3HA T- with the catalytic HAT domain deleted had no complementation ability. These results implicate that human Elp3 subunit had similar functions to those of the yeast, and the HAT activity of human Elp3 was essential for its function.
Li
Fen
F
Institute of Genetics and Cytology, Northeast Normal University, Changchun.
Han
Qiu Ju
QJ
Lu
Jun
J
Huang
Bai Qu
BQ
D000135:Acid Phosphatase / Q000235:genetics; D000595:Amino Acid Sequence; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test / Q000379:methods*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D051548:Histone Acetyltransferases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013696:Temperature
D018840:HSP70 Heat-Shock Proteins; D009419:Nerve Tissue Proteins; C090869:SSA3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C538729:ELP3 protein, human; C526363:Elp3 protein, S cerevisiae; D051548:Histone Acetyltransferases; D000135:Acid Phosphatase; C488916:PHO5 protein, S cerevisiae