title: Thermolabile variant, PHOX-S, of prophenol oxidase in Drosophila melanogaster.
abstract: The Phox(S) strain of Drosophila melanogaster is an electrophoretically slow variant found in a wild population at Victoria, Australia. Prophenol oxidase isoform A(1) from PHOX-S was purified and characterized biochemically and genetically. The purified fraction of A(1) from PHOX-S showed a homodimer with a molecular weight of the subunit of approximately 77 kDa. The Phox(S) strain was temperature sensitive in vivo in culture, and the purified protein was thermolabile in vitro. By the deletion mapping method, the Phox(S) locus was cytologically estimated to be at the location 55-A on the right arm of the second chromosome and 79.6 genetically. These data show that PHOX-S is an electrophoretic variant of MOX and that PHOX-S is the first thermolabile protein found in invertebrate prophenol oxidase.
authors.affiliation: Biological Laboratory, Faculty of Science, Okayama University of Science, Okayama, 700-0005, Japan. asada@das.ous.ac.jp
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Asada
authors.forename: Satoko
authors.initials: S
authors.lastname: Kitamura
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Tanaka
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Namba
mesh_terms: D000818:Animals; D004156:Catechol Oxidase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D002874:Chromosome Mapping; D003080:Cold Temperature; D029721:Drosophila Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D006358:Hot Temperature; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007814:Larva / Q000201:enzymology / Q000235:genetics; D008297:Male; D011679:Pupa / Q000201:enzymology / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; D004792:Enzyme Precursors; D007527:Isoenzymes; C038451:pro-phenoloxidase; D004156:Catechol Oxidase