title: Metabolism of Linoleic Acid or Mevalonate and 6-Pentyl-alpha-Pyrone Biosynthesis by Trichoderma Species.
abstract: The understanding of the biosynthetic pathway of 6-pentyl-alpha-pyrone in Trichoderma species was achieved by using labelled linoleic acid or mevalonate as a tracer. Incubation of growing cultures of Trichoderma harzianum and T. viride with [U-C]linoleic acid or [5-C]sodium mevalonate revealed that both fungal strains were able to incorporate these labelled compounds (50 and 15%, respectively). Most intracellular radioactivity was found in the neutral lipid fraction. At the initial time of incubation, the radioactivity from [C]linoleic acid was incorporated into 6-pentyl-alpha-pyrone more rapidly than that from [C]mevalonate. No radioactivity incorporation was detected in 6-pentyl-alpha-pyrone when fungal cultures were incubated with [1-C]linoleic acid. These results suggested that beta-oxidation of linoleic acid was a probable main step in the biosynthetic pathway of 6-pentyl-alpha-pyrone in Trichoderma species.
authors.affiliation: Laboratoire de Biotechnologie, Université de Bourgogne, ENS BANA, 1 Esplanade Erasme, F-21000 Dijon, France.
authors.forename: L
authors.initials: L
authors.lastname: Serrano-Carreon
authors.forename: Y
authors.initials: Y
authors.lastname: Hathout
authors.forename: M
authors.initials: M
authors.lastname: Bensoussan
authors.forename: J M
authors.initials: JM
authors.lastname: Belin