Utilization of Glycerol as a Hydrogen Acceptor by Lactobacillus reuteri: Purification of 1,3-Propanediol:NAD Oxidoreductase.
Lactobacillus reuteri utilizes exogenously added glycerol as a hydrogen acceptor during carbohydrate fermentations, resulting in higher growth rates and cell yields than those obtained during growth on carbohydrates alone. Glycerol is first converted to 3-hydroxypropionaldehyde by a coenzyme B(12)-dependent glycerol dehydratase and then reduced to 1,3-propanediol by an NAD -dependent oxidoreductase. The latter enzyme was purified and determined to have a molecular weight of 180,000; it is predicted to exist as a tetramer of identical 42,000-molecular-weight subunits.
Talarico
T L
TL
Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695, and Department of Microbiology, Swedish University of Agricultural Sciences, S-75007 Uppsala, Sweden.
Axelsson
L T
LT
Novotny
J
J
Fiuzat
M
M
Dobrogosz
W J
WJ