title: Metabolism of Pyrene by the Basidiomycete Crinipellis stipitaria and Identification of Pyrenequinones and Their Hydroxylated Precursors in Strain JK375.
abstract: The metabolism of pyrene, a polycyclic aromatic hydrocarbon, by submerged cultures of the basidiomycete Crinipellis stipitaria was studied. After incubation for 68 h at 25 degrees C in a 20-liter fermentor with complex medium and 20 mg of pyrene per liter, five metabolites were detected. The compounds were isolated by preparative high-performance liquid chromatography on RP18 and DIOL gels. By UV, infrared, and H nuclear magnetic resonance spectroscopy and mass spectrometry, 1-hydroxypyrene, 1,6-dihydroxypyrene, 1,8-dihydroxypyrene, 1,6-pyrenequinone, and 1,8-pyrenequinone were identified. 1,6- and 1,8-dihydroxypyrene were obtained from fungal cultures for the first time. The formation of these metabolites was confirmed by investigations with [4,5,9,10-C]pyrene.
authors.affiliation: Department of Biotechnology, University of Kaiserslautern, D-67663 Kaiserslautern, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Lambert
authors.forename: S
authors.initials: S
authors.lastname: Kremer
authors.forename: O
authors.initials: O
authors.lastname: Sterner
authors.forename: H
authors.initials: H
authors.lastname: Anke