title: [Rapid separation and determination of enterotoxigenic E. coli by capillary zone electrophoresis].
abstract: Rapid separation and determination of enterotoxigenic E. coli K88, K99 and 987P with intact cells using capillary zone electrophoresis (CZE) are described. The CZE was performed in a running buffer of 0.05 mol/L Na2CO3-NaHCO3 (pH 9.9), under applied voltage of 14.1 kV and detected by an on-line UV at 210 nm. Results show that K88, K99 and 987P were separated completely. Moreover, each strain presented a characteristic peak and a reproducible retention time (RSD < or = 0.9%). The successful analysis of enterotoxigenic E. coli in the excrement of diarrhea piglets is also discussed. K88 and K99 were detected in the excrement of diarrhea piglets born in 5 d - 6 d, and 30 d - 35 d respectively, and nothing was detected in the excrement of diarrhea piglets born in about 60 d.
authors.affiliation: Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China. chen77ping@163.com
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Ren-Kuan
authors.initials: RK
authors.lastname: Li
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Xu
authors.forename: Ping-Fan
authors.initials: PF
authors.lastname: Rao
mesh_terms: D000818:Animals; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D019075:Electrophoresis, Capillary / Q000379:methods*; D054307:Enterotoxigenic Escherichia coli / Q000737:chemistry* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*