Studies on interaction of norfloxacin, Cu2+, and DNA by spectral methods.
The interactions of norfloxacin (NFA), DNA, and Cu2+ are studied by fluorescence and UV-spectra method. According to the experimental results, it can be concluded that NFA can form a steady binary complex with Cu2+. There is a linear relationship between the Fluorescence intensity of the norfloxacin-Cu2+-DNA system and the concentration of DNA. And when the concentration of the NFA is 1.95x10(-5) mol L-1, they possess a good linearity in the concentration of DNA ranged from 4.7x10(-6) to 2.8x10(-5) mol L-1. It is a good method due to the high sensitivity and selectivity.
Song
Gongwu
G
Faculty of Chemistry and Material Science, Hubei University, Wuhan, 430062, People's Republic of China. songgw@hubu.edu.cn
Yan
Qiujun
Q
He
Yu
Y
D002138:Calibration; D002413:Cations, Divalent / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009643:Norfloxacin / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009994:Osmolar Concentration; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D002413:Cations, Divalent; D003300:Copper; D004247:DNA; D009643:Norfloxacin