title: Differential beta-arrestin binding of AT1 and AT2 angiotensin receptors.
abstract: Agonist stimulation of G protein-coupled receptors causes receptor activation, phosphorylation, beta-arrestin binding and receptor internalization. Angiotensin II (AngII) causes rapid internalization of the AT1 receptors, whereas AngII-bound AT2 receptors do not internalize. Although the activation of the rat AT1A receptor with AngII causes translocation of beta-arrestin2 to the receptor, no association of this molecule with the AT2 receptor can be detected after AngII treatment with confocal microscopy or bioluminescence resonance energy transfer. These data demonstrate that the two subtypes of angiotensin receptors have different mechanisms of regulation.
authors.affiliation: Department of Physiology, Semmelweis University, Faculty of Medicine, H-1444 Budapest, P.O. Box 259, Budapest, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Turu
authors.forename: László
authors.initials: L
authors.lastname: Szidonya
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Gáborik
authors.forename: László
authors.initials: L
authors.lastname: Buday
authors.forename: András
authors.initials: A
authors.lastname: Spät
authors.forename: Adrian J L
authors.initials: AJ
authors.lastname: Clark
authors.forename: László
authors.initials: L
authors.lastname: Hunyady
mesh_terms: D000804:Angiotensin II / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D019390:Arrestins / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D018613:Microscopy, Confocal; D010766:Phosphorylation / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D021381:Protein Transport / Q000187:drug effects / Q000502:physiology; D051381:Rats; D044140:Receptor, Angiotensin, Type 1 / Q000819:agonists* / Q000378:metabolism; D044139:Receptor, Angiotensin, Type 2 / Q000819:agonists* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D014662:Vasoconstrictor Agents / Q000378:metabolism / Q000494:pharmacology*; D000071557:beta-Arrestins
chemical_list: D019390:Arrestins; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D014662:Vasoconstrictor Agents; D000071557:beta-Arrestins; D000804:Angiotensin II