Protection of sediment-adsorbed transforming DNA against enzymatic inactivation.
The action of DNase I on transforming DNA, both adsorbed to marine sediment and in solution, was investigated. DNase I reduced the transformation frequencies of free DNA more than of adsorbed DNA. Changes in salt concentration or pH did not have a significant influence on the DNA-sediment complex. Soil components other than organic materials and clay minerals can bind DNA and retard its enzymatic degradation.
Aardema
B W
BW
Geomicrobiology Division, University of Oldenburg, D-2900 Oldenburg, West Germany.
Lorenz
M G
MG
Krumbein
W E
WE