Picrotoxin-induced convulsions alters rat brain microsomal membrane structural properties.
Cerebral microsomal membrane properties were assessed in the chronic condition of generalized seizure induced by picrotoxin (PTX) in rats. PTX-induced seizures resulted in increased lysophosphatidyl glycerol, phosphatidylcholine and phosphatidic acid components, while phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol were significantly reduced by 19-73%. The cholesterol (CHL) content increased considerably by 25% without alteration in total phospholipids content. Microsomal membrane was more fluidized in the epileptic condition. Possible consequences of microsomal membrane alterations are discussed in terms of deregulation of Ca2+ homeostasis. In conclusion, alterations in the microsomal membrane properties may have a significant influence on the cerebral function in the chronic epileptic condition.
Acharya
Munjal M
MM
Department of Biochemistry and Biotechnology, Institute of Science, Nirma University of Science and Technology, Sarkhej-Gandhinagar Highway, Ahmedabad 382481, Gujarat, India. munjalres@yahoo.com
Katyare
Surendra S
SS
D000818:Animals; D001921:Brain / Q000166:cytology*; D002784:Cholesterol / Q000378:metabolism; D004195:Disease Models, Animal; D008297:Male; D008566:Membranes / Q000187:drug effects* / Q000378:metabolism; D008861:Microsomes / Q000473:pathology*; D010743:Phospholipids / Q000378:metabolism; D010852:Picrotoxin; D051381:Rats; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology
D002784:Cholesterol; D010743:Phospholipids; D010852:Picrotoxin