[Rapid diagnosis of donor infection and renal allograft contamination using a polymerase chain reaction technique and rapid shaking culture].
Post transplant infection is one of the serious complications of the organ recipients. We detected the donor infections and allograft contaminations in a limited period of time by polymerase chain reaction (PCR) and rapid shaking culture (RSC). The pre-procurement blood from 86 possible renal donors as well as the preservation solution (PS) and renal pelvic urine (PU) from 158 grafts were examined in order to detect highly virulent organisms such as methicillin resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and fungi. The average age of donors was 48.8 years old. The average period between the admission and brain death was 4.9 days, and the average period between the brain death and cardiac arrest was 3.6 days. The inflammatory indices such as body temperature, white blood cell count and C-reactive protein increased to 37.9 +/- 1.1 degree C, 12,600 +/- 6300/microl, and 20.2 +/- 11.6 mg/dl, respectively. Following PCR and RSC, procurement operations of the three possible donors were cancelled because of systemic bacterial infections by MRSA or Bacteroides fragilis. Six out of 158 grafts were discarded due to the diagnoses of MRSA or Candida albicans in PS and/or PU. All the other 148 grafts were transplanted. Following transplant, no single infectious complication transmitted by the graft was noted. We conclude that PCR combined with RSC is very accurate and useful for detecting donor infections and allograft contaminations, which may cause severe complications in the recipients.
Naitoh
Kazuhiko
K
The Department of Urology, Fujita Health University School of Medicine.
Higuchi
Toru
T
Sasaki
Hitomi
H
Kuwahara
Yoshitaka
Y
Kusaka
Mamoru
M
Ishikawa
Kiyohito
K
Shiroki
Ryoichi
R
Hoshinaga
Kiyotaka
K
D001419:Bacteria / Q000235:genetics; D001424:Bacterial Infections / Q000517:prevention & control* / Q000635:transmission; D002102:Cadaver; D018929:Cell Culture Techniques; D005260:Female; D006801:Humans; D007668:Kidney / Q000382:microbiology / Q000473:pathology*; D016030:Kidney Transplantation; D008297:Male; D016106:Methicillin Resistance; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000175:diagnosis; D013211:Staphylococcus aureus / Q000302:isolation & purification; D014019:Tissue Donors / Q000145:classification