title: Extracellular Enzyme Production and Synthetic Lignin Mineralization by Ceriporiopsis subvermispora.
abstract: The ability of the white rot fungus Ceriporiopsis subvermispora to mineralize C-synthetic lignin was studied under different culture conditions, and the levels of two extracellular enzymes were monitored. The highest mineralization rates (28% after 28 days) were obtained in cultures containing a growth-limiting amount of nitrogen source (1.0 mM ammonium tartrate); under this condition, the levels of manganese peroxidase (MnP) and laccase present in the culture supernatant solutions were very low compared with cultures containing 10 mM of the nitrogen source. In contrast, cultures containing a limiting concentration of the carbon source (0.1% glucose) showed low levels of both enzymes and also very low mineralization rates compared with cultures containing 1% glucose. Cultures containing 11 ppm of Mn(II) showed a higher rate of mineralization than those containing 0.3 or 40 ppm of this cation. Levels of MnP and laccase were higher when 40 ppm of Mn(II) was used. Mineralization rates were slightly higher in cultures flushed daily with oxygen, whereas laccase levels were lower and MnP levels were approximately the same as in cultures maintained under an air atmosphere. The presence of 0.4 mM veratryl alcohol reduced both mineralization rates and MnP levels, without affecting laccase levels. Lignin peroxidase activity was not detected under any condition. Addition of purified lignin peroxidase to the cultures in the presence or absence of veratryl alcohol did not enhance mineralization rates significantly.
authors.affiliation: Laboratorio de Bioquímica, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile, and Institute for Microbial and Biochemical Technology, Forest Products Laboratory, Forest Service, U.S. Department of Agriculture, Madison, Wisconsin 53705-2398.
authors.forename: C
authors.initials: C
authors.lastname: Rüttimann-Johnson
authors.forename: L
authors.initials: L
authors.lastname: Salas
authors.forename: R
authors.initials: R
authors.lastname: Vicuña
authors.forename: T K
authors.initials: TK
authors.lastname: Kirk