Selection of cell source for ligament tissue engineering.
Use of appropriate types of cells could potentially improve the functionality and structure of tissue engineered constructs, but little is known about the optimal cell source for ligament tissue engineering. The object of this study was to determine the optimal cell source for anterior cruciate ligament (ACL) tissue engineering. Fibroblasts isolated from anterior cruciate ligament, medial collateral ligament (MCL), as well as bone marrow mesenchymal stem cells (MSC) were compared using the following parameters: proliferation rate, collagen excretion, expression of collagen type I, II, and III, as well as alpha-smooth muscle actin. Green fluorescent protein (GFP) transfected MSCs were used to trace their fate in the knee joints. MSC, ACL, and MCL fibroblasts were all highly stained with antibodies for collagen types I and III and alpha-smooth muscle actin while negatively stained with collagen type II. Proliferation rate and collagen excretion of MSCs were higher than ACL and MCL fibroblasts (p < 0.05), and MSCs could survive for at least 6 weeks in knee joints. In summary, MSC is potentially a better cell source than ACL and MCL fibroblasts for anterior cruciate ligament tissue engineering.
Ge
Zigang
Z
Department of Orthopaedic Surgery, National University of Singapore.
Goh
James Cho Hong
JC
Lee
Eng Hin
EH
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000166:cytology; D001854:Bone Marrow Cells; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D007719:Knee Joint / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D014162:Transfection
D003094:Collagen