A derivative of Mycobacterium smegmatis mc(2)155 that lacks the duplicated chromosomal region.
The genome of Mycobacterium smegmatis mc(2)155 contains a 56kb duplicated region. We isolated a mutant of mc(2)155 lacking this duplication (DeltaDRKIN). This mutation did not affect the growth rate, surface properties or transformation efficiency of the organism, confirming the potential utility of DeltaDRKIN for the study of genes contained within the duplicated region.
Warner
Digby F
DF
MRC/NHLS/WITS Molecular Mycobacteriology Research Unit, DST-NRF, School of Pathology of the University of the Witwatersrand, Johannesburg, South Africa. digby.warner@nhls.ac.za <digby.warner@nhls.ac.za>
Etienne
Gilles
G
Wang
Xiao-Ming
XM
Matsoso
Limenako G
LG
Dawes
Stephanie S
SS
Soetaert
Karine
K
Stoker
Neil G
NG
Content
Jean
J
Mizrahi
Valerie
V
D020440:Gene Duplication; D005798:Genes, Bacterial; D020102:Mycobacterium smegmatis / Q000235:genetics* / Q000254:growth & development; D016366:Open Reading Frames; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D013499:Surface Properties; D014170:Transformation, Genetic