title: [Contents of the glucose transporters GLUT1 and GLUT4 in oxidative and glycolytic muscles of goat kids and adult goats].
abstract: The objective of this study was to elucidate, whether the impaired insulin sensitivity with regard to glucose utilisation in ruminating goats compared with suckling goat kids may be due to a reduced expression of the glucose transporters GLUT1 and GLUT4 in skeletal muscle. Muscle samples were removed from red, oxidative muscles (M. masseter, diaphragm) and white, glycolytic muscles (M. longissimus dorsi, and M. semitendinosus) of five goat kids fed with milk exchanger and nine adult goats of different stages of life. Samples were analysed for their GLUT1 and GLUT4 contents. The muscles were characterised metabolically by measuring the activities of isocitrate dehydrogenase (ICDH) and of lactate dehydrogenase. In all four analysed muscles the GLUT4 contents of adult goats were significantly (p < 0.05) lower than in goat kids. Significant differences concerning the GLUT4 contents in skeletal muscles were not detected in adult goats of different stages of life. The GLUT1 contents differed to a lower extend between goat kids and adult goats. The results of this investigation indicate that the impaired insulin sensitivity of adult compared with suckling ruminants is accompanied by or leads to a decreased GLUT4 expression.
authors.affiliation: Klinik für Kleine Klauentiere, Forensische Medizin und Ambulatorische Klinik, Stiftung Tierärztliche Hochschule Hannover. Reinhard.Duehlmeier@tiho-hannover.de
authors.forename: R
authors.initials: R
authors.lastname: Dühlmeier
authors.forename: J
authors.initials: J
authors.lastname: Voigt
authors.forename: G
authors.initials: G
authors.lastname: Breves
authors.forename: H P
authors.initials: HP
authors.lastname: Sallmann
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D005260:Female; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism*; D006019:Glycolysis; D006041:Goats / Q000378:metabolism*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase