Genome-wide analysis of the expression profile of Saccharomyces cerevisiae in response to treatment with the plant isoflavone, wighteone, as a potential antifungal agent.
The limitations of currently available antifungal agents and the rapid emergence of drug-resistant strains necessitate more efficient approaches to screening and developing novel antifungal drugs. The antifungal activity of the natural products of a series of plants was evaluated and wighteone, 5, 7, 4'-trihydroxy-6-(gamma,gamma-dimethylallyl)isoflavone showed excellent anti-yeast activity (MIC against Saccharomyces cerevisiae was 4 microg/ml). Transcriptome profiling of wighteone-treated S. cerevisiae indicated that wighteone is different from commonly used antifungal compounds in its mode of action.
Yin
Hongying
H
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
Zhao
Yongchao
Y
Zhang
Yan
Y
Zhang
Hongwu
H
Xu
Lizhen
L
Zou
Zhongmei
Z
Yang
Weiping
W
Cheng
Jing
J
Zhou
Yuxiang
Y
D000935:Antifungal Agents / Q000494:pharmacology*; D004341:Drug Evaluation; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D016681:Genome, Fungal; D007529:Isoflavones / Q000494:pharmacology*; D028321:Plant Preparations / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
D000935:Antifungal Agents; D007529:Isoflavones; D028321:Plant Preparations