title: Inhibition of chlamydiae by primary alcohols correlates with the strain-specific complement of plasticity zone phospholipase D genes.
abstract: Members of the genus Chlamydia are obligate intracellular pathogens that have a unique biphasic developmental cycle and interactions with host cells. Many genes that dictate host infection tropism and, putatively, pathogenic manifestations of disease are clustered in a hypervariable region of the genome termed the plasticity zone (PZ). Comparative genomics studies have determined that an uncharacterized family of PZ genes encoding orthologs of eukaryotic and prokaryotic members of the phospholipase D (PLD) enzyme family varies among chlamydiae. Here, we show that the PZ PLD (pzPLD) of Chlamydia trachomatis are transcribed during both normal and persistent infection and that the corresponding PLD proteins are predominantly localized in reticulate bodies on the inner leaflet of the inclusion membrane. Further, we show that strains of chlamydiae encoding the pzPLD, but not a strain lacking these genes, are inhibited by primary alcohols, potent PLD inhibitors, during growth in HeLa 229 cells. This inhibitory effect is amplified approximately 5,000-fold during recovery from persistent infection. These findings suggest that the chlamydial pzPLD may be important, strain-specific, pathogenesis factors in vivo.
authors.affiliation: Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, National Institutes of Health, 903 South 4th Street, Hamilton, MT 59840, USA.
authors.forename: David E
authors.initials: DE
authors.lastname: Nelson
authors.forename: Deborah D
authors.initials: DD
authors.lastname: Crane
authors.forename: Lacey D
authors.initials: LD
authors.lastname: Taylor
authors.forename: David W
authors.initials: DW
authors.lastname: Dorward
authors.forename: Morgan M
authors.initials: MM
authors.lastname: Goheen
authors.forename: Harlan D
authors.initials: HD
authors.lastname: Caldwell
mesh_terms: D000438:Alcohols / Q000494:pharmacology*; D002690:Chlamydia Infections / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000276:immunology*; D003165:Complement System Proteins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D010739:Phospholipase D / Q000235:genetics*; D013045:Species Specificity
chemical_list: D000438:Alcohols; D003165:Complement System Proteins; D010739:Phospholipase D