Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies.
OBJECTIVE
To test a newly invented intracytoplasmic sperm injection (ICSI) sperm selection method based on sperm hyaluronic acid (HA) binding.


METHODS
Comparison of chromosomal disomy and diploidy frequencies in sperm arising from semen and in HA-bound sperm.


METHODS
Academic andrology laboratory.


METHODS
Men presenting for semen analysis.


METHODS
Washed sperm fractions of 32 semen samples were applied to Petri dishes or glass slides coated with immobilized HA. The unbound sperm were rinsed gently, and the HA-bound sperm were removed with an ICSI pipette. The control sperm population was the unselected sperm. Both HA-selected and unselected sperm were treated with fluorescence in situ hybridization with centromeric probes for the X, Y, and 17 chromosomes.


METHODS
Chromosomal disomy and diploidy frequencies.


RESULTS
In the HA-bound sperm (495-2,079 per man, 41,670 in all) compared with unselected sperm (4,770 per man, 162,210 in all), the chromosomal disomy frequencies were reduced to 0.16% from 0.52%, diploidy to 0.09% from 0.51%, and sex chromosome disomy to 0.05% from 0.27% (a 5.4-fold reduction vs. 4-fold respective increase in ICSI offspring).


CONCLUSIONS
The HA sperm selection method for ICSI, which is based on a relationship between sperm receptors for zona pellucida and HA, will likely reduce the potential genetic complications and adverse public health effects of ICSI.
Jakab
Attila
A
Sperm Physiology Laboratory, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Sakkas
Denny
D
Delpiano
Elena
E
Cayli
Sevil
S
Kovanci
Ertug
E
Ward
David
D
Revelli
Alberto
A
Ravelli
Alberto
A
Huszar
Gabor
G
D033441:Andrology / Q000379:methods; D000782:Aneuploidy; D004171:Diploidy; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D066298:In Vitro Techniques; D008297:Male; D009845:Oligospermia / Q000473:pathology* / Q000628:therapy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism; D024182:Uniparental Disomy / Q000473:pathology
D006820:Hyaluronic Acid