title: Correlation between lethal toxin-neutralizing antibody titers and protection from intranasal challenge with Bacillus anthracis Ames strain spores in mice after transcutaneous immunization with recombinant anthrax protective antigen.
abstract: Transcutaneous immunization of mice with recombinant protective antigen (rPA) of Bacillus anthracis resulted in significantly higher lethal toxin-neutralizing antibody titers than did intramuscular injection of alum-adsorbed rPA. Immunized mice were partially protected against intranasal challenge with 235,000 (10 50% lethal doses) Ames strain B. anthracis spores. A highly significant correlation was observed between toxin-neutralizing antibody titer and survival after challenge. Future experiments with rabbits and nonhuman primates should confirm the significance of protection by this vaccine strategy.
authors.affiliation: Department of Vaccine Production and Delivery, Division of Retrovirology, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, 1600 East Gude Drive, Rockville, MD 20850, USA.
authors.forename: Kristina K
authors.initials: KK
authors.lastname: Peachman
authors.forename: Mangala
authors.initials: M
authors.lastname: Rao
authors.forename: Carl R
authors.initials: CR
authors.lastname: Alving
authors.forename: Robert
authors.initials: R
authors.lastname: Burge
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Leppla
authors.forename: Venigalla B
authors.initials: VB
authors.lastname: Rao
authors.forename: Gary R
authors.initials: GR
authors.lastname: Matyas
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000881:Anthrax / Q000276:immunology* / Q000517:prevention & control; D022122:Anthrax Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D001408:Bacillus anthracis / Q000235:genetics / Q000276:immunology*; D005260:Female; D051379:Mice; D008808:Mice, Inbred CBA; D013171:Spores, Bacterial / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D014614:Vaccines, Synthetic