Donor CD4+ T and B cells in transplants induce chronic graft-versus-host disease with autoimmune manifestations.
Chronic graft-vs-host disease (GVHD) is a major cause of morbidity and mortality of long-term survivors of allogeneic hemato-poietic cell transplantation (HCT). Chronic GVHD can have features of an autoimmune collagen vascular disease with clinical manifestations similar to autoimmune scleroderma and systemic lupus erythematosus (SLE). However, the pathogenesis of chronic GVHD is poorly understood. It is unclear how autoreactive T and B cells are generated in chronic GVHD recipients. We have recently developed a new chronic GVHD model by transplantation of donor DBA/2 (H-2d) spleen cells into major histocompatibility complex (MHC)-matched but minor antigen-mismatched sublethally irradiated BALB/c (H-2d) recipients as well as athymic BALB/c(nu/nu) and adult-thymectomized BALB/c recipients. Both euthymic and athymic BALB/c recipients developed high levels of serum IgG autoantibodies, sclerodermatous skin damage, and glomerulonephritis. Disease induction required both donor CD25-CD4+ T and B cells in transplants. In contrast, donor CD25+CD4+ T regulatory (Treg) cells prevented the disease induction. These results indicate that host thymus is not required for induction of chronic GVHD and that quiescent autoreactive T and B cells in transplants from nonautoimmune donors may be activated and expanded to cause chronic GVHD with autoimmune manifestations in allogeneic recipients, and donor Treg cells can suppress this process.
Zhang
Chunyan
C
The Beckman Research Institute, Gonda Building, R2017, City of Hope National Medical Center, 1500 East Duarte Rd, Duarte, CA 91010, USA.
Todorov
Ivan
I
Zhang
Zhifang
Z
Liu
Yinping
Y
Kandeel
Fouad
F
Forman
Stephen
S
Strober
Samuel
S
Zeng
Defu
D
D000818:Animals; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D001402:B-Lymphocytes / Q000637:transplantation*; D015496:CD4-Positive T-Lymphocytes / Q000637:transplantation*; D004195:Disease Models, Animal; D006086:Graft vs Host Disease / Q000097:blood / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation; D017710:Lymphocyte Transfusion / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D013154:Spleen / Q000276:immunology; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D014184:Transplantation, Homologous