Preclinical metabolic changes in mouse prion diseases detected by 1H-nuclear magnetic resonance spectroscopy.
Magnetic resonance spectroscopy studies in animal models of prion disease are very few and concern terminal stages of infection. In order to study earlier stages of the disease, we used in-vivo magnetic resonance spectroscopy in a mouse model of scrapie and, for the first time, in mice infected with a bovine spongiform encephalopathy strain. In bovine spongiform encephalopathy-infected mice, we observed an increase in myo-inositol preceding clinical signs by 20 days, followed by a decrease in N-acetylaspartate at advanced stages. In scrapie-infected mice, changes in N-acetylaspartate and myo-inositol were detected at the beginning of the symptomatic phase. These results show that magnetic resonance spectroscopy is a valuable tool for detecting subtle metabolic changes associated to gliosis and neuronal dysfunction in prion diseases.
Vidal
Catherine
C
CEA (Atomic Energy Commission), Laboratory of Neurovirology, Fontenay-aux-Roses, France. catherine.vidal@cea.fr
Méric
Philippe
P
Provost
Fanny
F
Herzog
Christian
C
Lasmézas
Corinne
C
Gillet
Brigitte
B
Beloeil
Jean-Claude
JC
Dormont
Dominique
D
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002417:Cattle; D004195:Disease Models, Animal; D005260:Female; D007239:Infections / Q000150:complications; D008279:Magnetic Resonance Imaging / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008810:Mice, Inbred C57BL; D017096:Prion Diseases / Q000378:metabolism* / Q000473:pathology; D012608:Scrapie / Q000378:metabolism*; D013997:Time Factors
D001224:Aspartic Acid; C000179:N-acetylaspartate