title: In-vitro analysis of APA microcapsules for oral delivery of live bacterial cells.
abstract: Oral administration of microcapsules containing live bacterial cells has potential as an alternative therapy for several diseases. This article evaluates the suitability of the alginate-poly-L-lysine-alginate (APA) microcapsules for oral delivery of live bacterial cells, in-vitro, using a dynamic simulated human gastro-intestinal (GI) model. Results showed that the APA microcapsules were morphologically stable in the simulated stomach conditions, but did not retain their structural integrity after a 3-day exposure in simulated human GI media. The microbial populations of the tested bacterial cells and the activities of the tested enzymes in the simulated human GI suspension were not substantially altered by the presence of the APA microcapsules, suggesting that there were no significant adverse effects of oral administration of the APA microcapsules on the flora of the human gastrointestinal tract. When the APA microcapsules containing Lactobacillus plantarum 80 (LP80) were challenged in the simulated gastric medium (pH = 2.0), 80.0% of the encapsulated cells remained viable after a 5-min incubation; however, the viability decreased considerably (8.3%) after 15 min and dropped to 2.6% after 30 min and lower than 0.2% after 60 min, indicating the limitations of the currently obtainable APA membrane for oral delivery of live bacteria. Further in-vivo studies are required before conclusions can be made concerning the inadequacy of APA microcapsules for oral delivery of live bacterial cells.
authors.affiliation: Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Physiology, Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Ouyang
authors.forename: M
authors.initials: M
authors.lastname: Jones
authors.forename: T
authors.initials: T
authors.lastname: Haque
authors.forename: B
authors.initials: B
authors.lastname: Lawuyi
authors.forename: S
authors.initials: S
authors.lastname: Prakash
mesh_terms: D000284:Administration, Oral; D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules / Q000008:administration & dosage; D004339:Drug Compounding / Q000379:methods; D005744:Gastric Acid; D005753:Gastric Mucosa / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000201:enzymology / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000254:growth & development; D008954:Models, Biological; D011107:Polylysine / Q000031:analogs & derivatives*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; C051546:alginate-polylysine-alginate; D011107:Polylysine