The adrenal gland is a source of stress-induced circulating IL-18.
The present study compared plasma IL-18 levels between sham-operated and adrenalectomized mice following stress to investigate whether the adrenal gland contributes to the elevation of circulating IL-18 during stress. Two hours of stress provoked a robust, stressor-dependent, elevation of IL-18 mRNA and peptide in the adrenal gland in sham-operated mice. Consistently, levels of circulating mature IL-18 increased during stress and remained elevated for up to 6 h after stress. The stress-induced increase in circulating IL-18 was abolished by adrenalectomy. These findings demonstrate that the adrenal gland is required to achieve elevation of circulating IL-18 during stress.
Sugama
Shuei
S
Department of Physiology, Nippon Medical School, 1-1-5 Sendagi Bunkyo-ku, Tokyo 113-8602, Japan. sugama@nms.ac.jp
Wang
Ning
N
Shimokawa
Noriaki
N
Koibuchi
Noriyuki
N
Fujita
Masayo
M
Hashimoto
Makoto
M
Dhabhar
Firdaus S
FS
Conti
Bruno
B
D000311:Adrenal Glands / Q000378:metabolism*; D000315:Adrenalectomy / Q000379:methods; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology; D007101:Immersion / Q000009:adverse effects; D007150:Immunohistochemistry / Q000379:methods; D016209:Interleukin-8 / Q000097:blood* / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000096:biosynthesis; D011863:Radioimmunoassay / Q000379:methods; D012149:Restraint, Physical / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013312:Stress, Physiological / Q000378:metabolism*; D013997:Time Factors
D016209:Interleukin-8; D012333:RNA, Messenger; D000324:Adrenocorticotropic Hormone