title: Simultaneous observation of enzyme surface diffusion and surface reaction using microfluidic patterning of substrate surfaces.
abstract: We present a study of the simultaneous observation of protease reaction and surface diffusion as the enzyme interacts with a model substrate surface. We use micro-fluidic patterning to decorate a bovine serum albumin substrate surface with stripes of adsorbed enzyme in the absence of physical barriers. Spreading of the enzyme from the initial striped region indicates surface diffusion, while removal of the substrate provides a measure of reactivity. Microfluidic patterning provides a means to determine the relative importance of enzyme adsorption, surface diffusion, and reaction on the rate of substrate removal.
authors.affiliation: Department of Chemical Engineering, Stanford University, Stanford, California 94305-5025, USA.
authors.forename: Shaunak
authors.initials: S
authors.lastname: Roy
authors.forename: Jerry M
authors.initials: JM
authors.lastname: Thomas
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Holmes
authors.forename: James T
authors.initials: JT
authors.lastname: Kellis
authors.forename: A J
authors.initials: AJ
authors.lastname: Poulose
authors.forename: Channing R
authors.initials: CR
authors.lastname: Robertson
authors.forename: Alice P
authors.initials: AP
authors.lastname: Gast
mesh_terms: D000818:Animals; D002417:Cattle; D004058:Diffusion; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D020860:Subtilisin / Q000378:metabolism; D013499:Surface Properties
chemical_list: D004798:Enzymes; D012710:Serum Albumin, Bovine; D020860:Subtilisin