Fluorimetric determination of histamine in fish using micellar media and fluorescamine as labelling reagent.
An analytical method based on the use of fluorescamine to produce a fluorescent derivative with histamine and combined with micellar-enhanced fluorescence detection of the formed complex is developed for the sensitive and rapid determination of histamine in fishes. The fluorescence properties of the obtained complex in water and micellar solutions of sodium dodecyl sulfate (SDS), cetyltrimethylammonium chloride (CTAC) and brij-700 are reported. Physicochemical variables influencing the sensitivity of the method (pH, micellar, fluorescamine and NaCl relative concentrations) have been optimized. The stability of the formed complex, as shown by kinetic study, depends on the pH of the solution. Linear calibration curves allowing an effective histamine determination were established with large linear dynamic range (LDR), and low limits of detection (LOD) between 0.5 and 33 ng mL-1, according to the solvent. Application to the analysis of fish samples (sardines) yielded satisfactory results. The method seems to be suitable for environmental fish quality control.
Adamou
Rabani
R
Faculté des Sciences et Techniques, Département de Chimie, Laboratoire de Photochimie et d'Analyse, Université Cheikh Anta Diop, Dakar, Sénégal.
Coly
Atanasse
A
Douabalé
Stephy Edgar
SE
Saleck
Med Lemine Ould Cheikh Ould
ML
Gaye-Seye
Mame Diabou
MD
Tine
Alphonse
A
D000818:Animals; D005399:Fishes; D005450:Fluorescamine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006632:Histamine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014867:Water / Q000737:chemistry
D005456:Fluorescent Dyes; D008823:Micelles; D014867:Water; D005450:Fluorescamine; D006632:Histamine