Polymorphism of RANTES chemokine gene promoter is not associated with long-term nonprogressive HIV-1 infection of more than 16 years.
To examine whether polymorphisms of the RANTES chemokine gene promoter are associated with long-term nonprogressive HIV-1 infection in white Spanish subjects, we performed a cross-sectional genetic association case-control study. Two-hundred sixty-seven white Spaniards were studied: 58 were HIV-1-infected long-term nonprogressors (LTNPs) of more than 16 years, 109 were HIV-1-infected usual progressors (UPs), and 100 were control subjects. Three RANTES single nucleotide polymorphisms (SNPs) at positions -28C>G, -109T>C, and -403G>A were assessed. The prevalence of the CCR5Delta 32 allele was also examined. Genotyping was performed using polymerase chain reaction and automatic sequencing analysis methods. Genotype and allele frequencies between the 3 groups were compared by the chi2 test and the Fisher exact test. The distribution of allelic variants of RANTES in controls, UPs, and LTNPs, respectively, was 3%, 2%, and 5% for -28G; 4%, 2%, and 2% for -109C; and 18%, 18%, and 18% for -403A (P = not significant). The differences were still nonsignificant when we exclusively analyzed individuals not carrying the CCR5Delta32 allele. We conclude that LTNP of more than 16 years is not associated with SNPs in the RANTES gene promoter in white Spanish HIV-1-infected subjects.
Vidal
Francesc
F
Hospital Universitari de Tarragona Joan XXIII, Universitat Rovira i Virgili, Tarragona, Spain. fvidal@comt.es
Peraire
Joaquim
J
Domingo
Pere
P
Broch
Montserrat
M
Cairó
Mireia
M
Pedrol
Enric
E
Montero
Milagros
M
Viladés
Consuelo
C
Gutiérrez
Cristina
C
Sambeat
María Antònia
MA
Fontanet
Angels
A
Dalmau
David
D
Deig
Elisabeth
E
Knobel
Hernando
H
Sirvent
Joan Josep
JJ
Richart
Cristóbal
C
Veloso
Sergi
S
Saumoy
Maria
M
López-Dupla
Miguel
M
Olona
Montserrat
M
Cadafalch
Josep
J
Fuster
Montserrat
M
Ochoa
Anna
A
Soler
Anna
A
Guelar
Ana
A
González
Judit
J
D000328:Adult; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000235:genetics*; D018925:Chemokines / Q000235:genetics; D003430:Cross-Sectional Studies; D018450:Disease Progression; D005260:Female; D015658:HIV Infections / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D013030:Spain; D013997:Time Factors
D018946:Chemokine CCL5; D018925:Chemokines