Radioisotopic method for measuring cell division rates of individual species of diatoms from natural populations.
Silicon is an essential element for diatom frustule synthesis and is usually taken up only by dividing cells. With Ge, a radioactive analog of Si, the cell cycle marker event of frustule formation was identified for individual species of diatom. The frequency of cells within a population undergoing this division event was estimated, and the cell division rate was calculated. In laboratory cultures, these rates of cell division and those calculated from changes in cell numbers were similar. By dual labeling with Ge(OH)(4) and NaHCO(3), rates of cell division and photosynthesis were coincidently measured for diatoms both in laboratory cultures and when isolated from natural populations in estuarine, offshore, and polar environments. These techniques permit the coupling between photosynthesis and cell division to be examined in situ for individual species of diatom.
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Horn Point Laboratories, University of Maryland, Cambridge, Maryland 21613.