Association of the Phe206Leu allele of the L-selectin gene with coronary artery disease.
OBJECTIVE
The aim of this study was to assess the association between the L-selectin Phe206Leu polymorphism and coronary artery disease.


METHODS
A total of 322 patients (221 men and 101 women) with coronary artery disease in one or more vessels documented by angiography were studied; 157 subjects (85 men and 72 women) without atherosclerosis were included as controls. All subjects were genotyped for the L-selectin Phe206Leu gene polymorphism using polymerase chain reaction with sequence-specific primer (PCR-SSP). To assess disease severity, all patients were classified by numbers of coronary arteries with 50% stenosis.


RESULTS
A significantly increased frequency of the 206Leu mutant allele was observed in patients with coronary artery disease compared to the controls. The 206Leu allele frequency occurred in 42% of the patients with coronary artery disease compared to 30% of the controls (p<0.009). No association was found between the severity of coronary artery disease and the L-selectin Phe206Leu polymorphism.


CONCLUSIONS
Our findings suggest that carriage of L-selectin 206Leu mutant allele could contribute to susceptibility of Iranian individuals to contracting coronary artery disease.
Hajilooi
Mehrdad
M
Department of Immunology, Molecular Medicine Research Center, Hamedan University of Medical Sciences, Hamedan, Iran.
Tajik
Nader
N
Sanati
Arashmidos
A
Eftekhari
Hossein
H
Massoud
Ahmad
A
D000368:Aged; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007492:Iran / Q000453:epidemiology; D019041:L-Selectin / Q000235:genetics*; D007930:Leucine; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D010649:Phenylalanine
D019041:L-Selectin; D010649:Phenylalanine; D007930:Leucine