Effects of Nitrapyrin [2-Chloro-6-(Trichloromethyl) Pyridine] on the Obligate Methanotroph Methylosinus trichosporium OB3b.
Nitrapyrin inhibited growth, CH(4) oxidation, and NH(4) oxidation, but not the oxidation of CH(3)OH, HCHO, or HCOONa, by Methylosinus trichosporium OB3b, suggesting that nitrapyrin acts against the methane monooxygenase enzyme system. The inhibition of CH(4) oxidation could be reversed by repeated washing of nitrapyrin-inhibited cells, indicating that its effect is bacteriostatic. The addition of Cu did not release the inhibition. Methane oxidation was also inhibited by 6-chloro-2-picoline. These data suggest that the mode of action of nitrapyrin on M. trichosporium is different from that on chemoautotrophic NH(4) oxidizers or methanogens.
Topp
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Department of Microbiology, Macdonald College of McGill University, Ste. Anne de Bellevue, Quebec H9X 1C0, Canada.
Knowles
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R