Direct fluorescence detection of Pb2+ and Cd2+ by high-performance liquid chromatography using 1-(4-aminobenzyl)ethylenediamine-N,N,N',N'-tetraacetate as a pre-column derivatizing agent.
A highly sensitive HPLC with direct fluorescent detection (lambda(ex) = 235 nm, lambda(em) = 355 nm) was developed for Pb2+ and Cd2+ complexes with an aromatic polyaminocarboxylate, 1-(4-aminobenzyl)ethylenediamine-N,N,N',N'-tetraacetate as a pre-column derivatizing agent. A reversed phase partition column pretreated by a cationic surfactant was employed. Although this ligand forms thermodynamically stable complexes with various metal ions, only peaks of Pb2+ and Cd2+ were detected with the ligand-centered emission in the HPLC due to the emissive activity and kinetic stability in the dissociation reaction. The detection limits obtained were 1.5x10(-8) and 3.3x10(-9) mol l(-1) for Pb2+ and Cd2+, respectively.
Saito
Shingo
S
Department of Applied and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami, Hokkaido 090-8507, Japan. shingo@chem.kitami-it.ac.jp
Danzaka
Naoki
N
Hoshi
Suwaru
S
D000085:Acetates / Q000737:chemistry*; D002104:Cadmium / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005029:Ethylenediamines / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry*; D007854:Lead / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
C509200:1-(4-aminobenzyl)ethylenediamine-N,N,N',N'-tetraacetate; D000085:Acetates; D005029:Ethylenediamines; D007202:Indicators and Reagents; D002104:Cadmium; D007854:Lead