title: Cytoprotective effect of green tea extract and quercetin against hydrogen peroxide-induced oxidative stress.
abstract: In this study, we evaluated the cytoprotective effects of antioxidative substances in hydrogen peroxide (H2O2) treated Mel-Ab melanocytes. Tested substances include selenium, quercetin, green tea (GT) extract, and several vitamins (ascorbic acid, Trolox, and folic acid). Of these, both quercetin and GT extract were found to have strong cytoprotective effects on H2O2-induced cell death. We also examined additive effects, but no combination of two of any of the above substances was found to act synergistically against oxidative damage in Mel-Ab cells. Nevertheless, a multi-combination of GT extract, quercetin, and folic acid appeared to prevent cellular damage in a synergistic manner, which suggests that combinations of antioxidants may be of importance, and that co-treatment with antioxidants offers a possible means of treating vitiligo, which is known to be related to melanocyte oxidative stress.
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Korea.
authors.forename: Yun-Mi
authors.initials: YM
authors.lastname: Jeong
authors.forename: Yeong-Gon
authors.initials: YG
authors.lastname: Choi
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Kim
authors.forename: Seo-Hyoung
authors.initials: SH
authors.lastname: Park
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Yoon
authors.forename: Sun-Bang
authors.initials: SB
authors.lastname: Kwon
authors.forename: Eun-Sang
authors.initials: ES
authors.lastname: Park
authors.forename: Kyoung-Chan
authors.initials: KC
authors.lastname: Park
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D028244:Camellia / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020111:Chlorodiphenyl (54% Chlorine) / Q000494:pharmacology; D052244:Endocrine Disruptors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D008544:Melanocytes / Q000187:drug effects; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology*; D014815:Vitamins / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D052244:Endocrine Disruptors; D010936:Plant Extracts; D014815:Vitamins; D020111:Chlorodiphenyl (54% Chlorine); D011794:Quercetin; D006861:Hydrogen Peroxide