[HPLC fingerprint of Fritillaria hupehensis].
OBJECTIVE
To establish a fingerprint analysis method of Fritillaria hupehensis.


METHODS
fingerprint was performed by HPLC-ELSD. Hypersil ODS column was used; the mobile phase was composed of methanol (with 0.05% triethylamine) and water with gradient elution; flow rate was 1.0 mL x min(-1); recording time was 60 min; drift tube temperature was 75 degrees C; gas flow rate was 1.9 L x min(-1).


RESULTS
HPLC fingerprint of Fritillaria hupehensis was obtained.


CONCLUSIONS
A reliable method was provided for controlling the quality of Fritillaria hupehensis.
Zhang
Guo-Xin
GX
School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Li
Juan
J
Zhang
Peng
P
Ruan
Han-Li
HL
Zhang
Yong-Hui
YH
Pi
Hui-Fang
HF
Wu
Ji-Zhou
JZ
D000470:Alkaloids / Q000032:analysis*; D002595:Cevanes / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031410:Fritillaria / Q000737:chemistry*; D008027:Light; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity
D000470:Alkaloids; D002595:Cevanes; C047331:peiminine