title: A low-cost open-source SNP genotyping platform for association mapping applications.
abstract: Association mapping aimed at identifying DNA polymorphisms that contribute to variation in complex traits entails genotyping a large number of single-nucleotide polymorphisms (SNPs) in a very large panel of individuals. Few technologies, however, provide inexpensive high-throughput genotyping. Here, we present an efficient approach developed specifically for genotyping large fixed panels of diploid individuals. The cost-effective, open-source nature of our methodology may make it particularly attractive to those working in nonmodel systems.
authors.affiliation: Department of Ecology and Evolutionary Biology, University of California Irvine, CA 92697-2525, USA. sjm@uci.edu
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Macdonald
authors.forename: Tomi
authors.initials: T
authors.lastname: Pastinen
authors.forename: Anne
authors.initials: A
authors.lastname: Genissel
authors.forename: Theodore W
authors.initials: TW
authors.lastname: Cornforth
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Long
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000191:economics* / Q000379:methods*; D003362:Cost-Benefit Analysis; D004331:Drosophila melanogaster / Q000235:genetics; D049750:Genome, Insect / Q000235:genetics; D005838:Genotype; D009841:Oligonucleotides / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D009841:Oligonucleotides