title: Genetic characterization of European-Zebu composite bovine using RFLP markers.
abstract: A population of 370 European-Zebu composite beef heifers, consisting of six different breed compositions (A-F), were characterized genetically, using RFLP markers of luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) genes. Our objectives were to genetically characterize this population and to determine the structure and the genetic variability of this hybrid herd. The genotypes were determined through PCR, followed by digestion with restriction endonucleases. The PCR-RFLP analysis made it possible to identify the LHR and FSHR genotypes, as well as to characterize the degree of heterozygosis, which was high for all of the breed compositions, for both loci, except for two combinations for LHR (B and C). The observed heterozygosity (Ho) was lower than the expected heterozygosity (He) for compositions C (for LHR) and A and D (for FSHR); however, for the population as a whole, Ho was above He (with a mean of 57 versus 46%, respectively), reflecting the elevated genetic variability in this population and also the informative value of the RFLP markers, which could be useful for population genetic characterization studies. The analysis of the degree of genetic structure of this population, estimated by the Nei's statistic, for both loci, indicated an elevated total genetic diversity (HT = 47%), with most of this variability being due to intrapopulational diversity (HS = 46%), with a low degree of genetic differentiation among the six breed compositions (GST = 1.2%). The estimates generated by the Wright's F statistic indicated a non-endogamic population, with excess heterozygotes (FIT = -0.22), which was also observed at the intrapopulational level (FIS = -0.23). The results gave evidence that the genetic selection applied to this European-Zebu composite population did not affect the expected high genetic variability for this type of crossbreeding, which makes it possible to use these animals to obtain economically valuable productive and reproductive traits.
authors.affiliation: Grupo de Melhoramento Animal, Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo (FZEA/USP), 13636-000 Pirassununga, SP, Brazil. epmarson@usp.br
authors.forename: Erica Perez
authors.initials: EP
authors.lastname: Marson
authors.forename: José Bento Sterman
authors.initials: JB
authors.lastname: Ferraz
authors.forename: Flávio Vieira
authors.initials: FV
authors.lastname: Meirelles
authors.forename: Júlio César de Carvalho
authors.initials: JC
authors.lastname: Balieiro
authors.forename: Joanir Pereira
authors.initials: JP
authors.lastname: Eler
authors.forename: Luís Gustavo Girardi
authors.initials: LG
authors.lastname: Figueiredo
authors.forename: Gerson Barreto
authors.initials: GB
authors.lastname: Mourão
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics*; D005260:Female; D006580:Genetic Carrier Screening; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011962:Receptors, FSH / Q000235:genetics*; D011974:Receptors, LH / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D011962:Receptors, FSH; D011974:Receptors, LH