[Analysis of volatile constituents of root and rhizome of Asarum heterotropoides Fr. var. mandshuricum (Maxim.) Kitag. by gas chromatography-mass spectrometry].
The volatiles of root and rhizome of Asarum heterotropoides Fr. var. mandshuricum (Maxzim. ) Kitag. were extracted with n-hexane and analyzed by capillary gas chromatography-flame ionization detection and gas chromatography-mass spectrometry (GC-MS) with the same column HP-5 (30 m x 0.25 mm i.d.). Initial oven temperature was kept at 40 degrees C for 2 min, then raised to 160 degrees C at 3 degrees C /min, immediately raised to 250 degrees C at 6 degrees C /min, and kept 250 degrees C for 23 min. According to GC-MS analysis, 36 compounds were identified. The main components are safrole, methyleugenol, 3,5-dimethoxytoluene, pentadecane, N-isobutyldodecatetraenamide. Furthermore, the analysis results of root and rhizome were compared. The results indicate that the fundamental constituents are nearly the same, but the quantities of volatiles in root are richer than those in rhizome. In addition, 8 compounds are selected as the criteria of quality control, including 3-carene, 1,8-cineole, eucarvone, methyleugenol, safrole, myristicin, pentadecane and N-isobutyldodecatetraenamide.
Zhang
Feng
F
Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China.
Wang
Long-Xing
LX
Luo
Qian
Q
Xiao
Hong-Bin
HB
Liang
Xin-Miao
XM
Cai
Shao-Qing
SQ
D030023:Asarum / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D055549:Volatile Organic Compounds / Q000032:analysis*
D010936:Plant Extracts; D055549:Volatile Organic Compounds