title: CO(2) Incorporation and 4-Hydroxy-2-Methylbenzoic Acid Formation during Anaerobic Metabolism of m-Cresol by a Methanogenic Consortium.
abstract: The metabolism of m-cresol by methanogenic cultures enriched from domestic sewage sludge was investigated. In the initial studies, bromoethanesulfonic acid was used to inhibit methane production. This led to the accumulation of 4.0 +/- 0.8 mol of acetate per mol of m-cresol metabolized. These results suggested that CO(2) incorporation occurred because each molecule of m-cresol contained seven carbon atoms, whereas four molecules of acetate product contained a total of eight carbon atoms. To verify this, [C]bicarbonate was added to bromoethanesulfonic acid-inhibited cultures, and those cultures yielded [C]acetate. Of the label recovered as acetate, 89% was found in the carboxyl position. Similar cultures fed [methyl-C]m-cresol yielded methyl-labeled acetate. A C-labeled transient intermediate was detected in cultures given either m-cresol and [C]bicarbonate or bicarbonate and [methyl-C]m-cresol. The intermediate was identified as 4-hydroxy-2-methylbenzoic acid. In addition, another metabolite was detected and identified as 2-methylbenzoic acid. This compound appeared to be produced only sporadically, and it accumulated in the medium, suggesting that the dehydroxylation of 4-hydroxy-2-methylbenzoic acid led to an apparent dead-end product.
authors.affiliation: Departments of Microbiology and Health Services Administration and Community Medicine, University of Alberta, Edmonton, Alberta, Canada T6G 2E9.
authors.forename: D J
authors.initials: DJ
authors.lastname: Roberts
authors.forename: P M
authors.initials: PM
authors.lastname: Fedorak
authors.forename: S E
authors.initials: SE
authors.lastname: Hrudey