[Effect of glutamine on the expression of grp75 in PC12 cells].
Glutamine (Gln), a conditionally essential amino acid, can be a potential enhancer of the heat stress response. And glucose-regulated protein 75(grp75) is a member of the hsp family. To evaluate the effect of glutamine on the expression of grp75, PC12 cell was cultured with DMEM, glucose-free DMEM, DMEM within Gln, and glucose-free DMEM within Gln, and the expression of grp75 was detected by immunocytochemistry and western blot, the mRNA level of grp75 in the cells was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Data indicated that Gln can upregulated the expression of grp75 in PC12 cell line, and the effect is more significantly in PC12 cell which was glucose deprivation than the normal cell. To investigate the effect of Gln on PC12 cells under glucose deprivation, MTT method was used to monitor the cell viability after Gln treatment for the cell under glucose depriving. The experiments results showed that glutamine at a concentration range of 0.2-40 mmol/L significantly enhanced the cell viability in glucose-free DMEM. And the protective effect to grp75 low-expresssion on PC12 cells is markedly decreased.
Liu
Wen
W
Department of Cellular and Genetic Medicine Shanghai Medical College Fudan University, Shanghai.
Zhang
Yan
Y
Wu
Qing Feng
QF
Li
Jin Yan
JY
Zuo
Ji
J
D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000187:drug effects; D005973:Glutamine / Q000494:pharmacology*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; C018551:glucose-regulated proteins; D005973:Glutamine