title: [Expression of human phosphodiesterase 3A gene using baculovirus expression system in insect cell].
abstract: OBJECTIVE
  To investigate the expression of recombinant human phosphodiesterase 3A (HPDE3A) using baculovirus expression system in Tn cell line.
  
  
  METHODS
  The HPDE3A cDNA was recombined with baculovirus, and then the recombinant was transfected into Tn cell line. The expression of HPDE3A in Tn cell line was detected and identified by the RT-PCR, SDS-PAGE and Western blotting.
  
  
  RESULTS
  The recombinant HPDE3A protein was stably expressed in Tn cell line and detected by the distinct morphological changes of Tn cell, RT-PCR, SDS-PAGE and Western blotting using polyclonal antibody. The M(w) of the recombinant protein was about 120 kD.
  
  
  CONCLUSIONS
  Recombinant HPDE3A can be expressed in Tn cell line using the baculovirus expression system, and thus provided the basic material for studying its bioactivity and application in screening for HPDE3A inhibitor.
authors.affiliation: School of Medical Technology, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Yan
authors.forename: Wen-Rong
authors.initials: WR
authors.lastname: Xu
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Zhu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Ren-Ren
authors.initials: RR
authors.lastname: Fei
authors.forename: Xiao-Liang
authors.initials: XL
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Qian
authors.forename: Shun-Zu
authors.initials: SZ
authors.lastname: Hao
authors.forename: Wen-Bing
authors.initials: WB
authors.lastname: Wang
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D004591:Electrophoresis, Polyacrylamide Gel; D009036:Moths / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D011994:Recombinant Proteins; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; C517287:PDE3A protein, human