title: UV-vis spectroscopic study directly detecting inorganic phosphorus in urine and our reagent kit.
abstract: The determination of inorganic phosphorus in human urine is very important, since it has diagnostic value in some clinical cases. Here we apply a simple, sensitive and direct method to determine inorganic phosphorus in urine. This new ensemble is prepared by adding ytterbium chloride and pyrocatechol violet in a 2:1 molar ratio in an aqueous solution of 10 mM 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid buffer at pH 7.0. The addition of the urine sample turned the blue ensemble yellow and altered the UV-vis absorption spectra. The ensemble exhibits excellent selectivity for inorganic phosphorus over other constituents of urine. We validate the accuracy of our method by the standard procedure (molybdenum blue assay for phosphate). The detection results are basically consistent with normal excretion of phosphate. Furthermore, we fabricated a new kind of inorganic phosphorus reagent kit, which enables us to inspect phosphate concentrations of urine with the naked eye. Fit for all kinds of various clinic uses, our reagent kit is a hopeful substitute for the molybdenum reagent kit.
authors.affiliation: Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, 030006, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Yin
authors.forename: Fangjun
authors.initials: F
authors.lastname: Huo
authors.forename: Pin
authors.initials: P
authors.lastname: Yang
mesh_terms: D001557:Benzenesulfonates / Q000737:chemistry; D002021:Buffers; D002712:Chlorides / Q000737:chemistry; D010758:Phosphorus / Q000652:urine*; D011933:Reagent Kits, Diagnostic / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D015018:Ytterbium / Q000737:chemistry
chemical_list: D001557:Benzenesulfonates; D002021:Buffers; D002712:Chlorides; D011933:Reagent Kits, Diagnostic; C009134:pyrocatechol violet; D010758:Phosphorus; C044841:ytterbium trichloride; D015018:Ytterbium