Adenosine A2A receptors promote adenosine-stimulated wound healing in bronchial epithelial cells.
Adenosine produces a wide variety of physiological effects through the activation of specific adenosine receptors (A(1), A(2A), A(2B), A(3)). Adenosine, acting particularly at the A(2A) adenosine receptor (A(2A)AR), is a potent endogenous anti-inflammatory agent and sensor of inflammatory tissue damage. The complete healing of wounds is the final step in a highly regulated response to injury. Recent studies on epidermal wounds have identified the A(2A)AR as the main adenosine receptor responsible for altering the kinetics of wound closure. We hypothesized that A(2A)AR promotes wound healing in bronchial epithelial cells (BECs). To test this hypothesis, the human BEC line BEAS-2B and bovine BECs (BBECs) were used. Real-time RT-PCR of RNA from unstimulated BEAS-2B cells revealed transcriptional expression of A(1), A(2A), A(2B) and A(3) receptors. Western blot analysis of lysates from BEAS-2B cells and BBECs detected a single band at 44.7 kDa in both the BECs, indicating the presence of A(2A)AR. In a wound healing model, we found that adenosine stimulated wound repair in cultured BBECs in a concentration-dependent manner, with an optimal closure rate observed between 4 and 6 h. Similarly, the A(2A)AR agonist 5'-(N-cyclopropyl)carboxamidoadenosine (CPCA) augmented wound closure, with a maximal closure rate occurring between 4 and 6 h. Inhibition of A(2A)AR with ZM-241385, a known A(2A)AR antagonist, impeded wound healing. In addition, ZM-241385 also attenuated adenosine-mediated wound repair. Kinase studies revealed that adenosine-stimulated airway repair activates PKA by ligating A(2A)AR. Collectively, the data suggest that the A(2A)AR is involved in BEC adenosine-stimulated wound healing and may prove useful in understanding purinergic-mediated actions on airway epithelial repair.
Allen-Gipson
D S
DS
Department of Internal Medicine, Pulmonary, Critical Care, and Sleep Medicine Section, University of Nebraska Medical Center, 985815 Nebraska Medical Center, Omaha, NE 68198-5815, USA. dallengipson@unmc.edu
Wong
J
J
Spurzem
J R
JR
Sisson
J H
JH
Wyatt
T A
TA
D000241:Adenosine / Q000494:pharmacology*; D001980:Bronchi / Q000293:injuries* / Q000502:physiology* / Q000503:physiopathology; D002455:Cell Division; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D043705:Receptor, Adenosine A2A / Q000235:genetics / Q000502:physiology*; D020545:Respiratory Mucosa / Q000293:injuries* / Q000502:physiology / Q000503:physiopathology; D014158:Transcription, Genetic; D014227:Triazines / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*; D014947:Wounds and Injuries / Q000503:physiopathology*
D043705:Receptor, Adenosine A2A; D014227:Triazines; D014230:Triazoles; C097270:ZM 241385; D000241:Adenosine