Low titer maternal antibodies can both enhance and suppress B cell responses to a combined live attenuated human rotavirus and VLP-ISCOM vaccine.
We investigated effects of low titer (Lo) circulating MatAb on protection and immunogenicity of attenuated (Att) human rotavirus (HRV) priming and 2/6-virus-like particle (VLP)-immunostimulating complex (ISCOM) boosting (AttHRV/VLP) or VLP-ISCOM alone vaccines. LoMatAb had both enhancing and suppressing effects on B cell responses, depending on tissue, antibody isotype and vaccine. Differential effects of LoMatAb on IgA responses in different tissues suggest that LoMatAb did not suppress induction of IgA effector and memory B cells but impaired homing of these cells to secondary lymphoid or effector tissues, reducing IgA antibody secreting cells and antibodies at these sites. The AttHRV/VLP vaccine partially overcame LoMatAb suppression, conferred moderate protection against virulent HRV (as measured by reduced viral shedding and diarrhea) and represents a new candidate for rotavirus vaccines for both humans and animals.
Nguyen
Trang V
TV
Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691-4096, USA.
Yuan
Lijuan
L
Azevedo
Marli S P
MS
Jeong
Kwang-Il
KI
Gonzalez
Ana M
AM
Iosef
Cristiana
C
Lovgren-Bengtsson
Karin
K
Morein
Bror
B
Lewis
Peggy
P
Saif
Linda J
LJ
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology*; D006801:Humans; D016882:ISCOMs / Q000008:administration & dosage*; D007112:Immunity, Maternally-Acquired; D007156:Immunologic Memory; D007274:Injections, Intraperitoneal; D022243:Rotavirus Vaccines / Q000276:immunology*; D013552:Swine; D014613:Vaccines, Attenuated / Q000276:immunology; D014771:Virion / Q000276:immunology*; D017201:Virus Shedding
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016882:ISCOMs; D022243:Rotavirus Vaccines; D014613:Vaccines, Attenuated