Three-dimensional control of protein patterning in microfabricated devices.
We present a new procedure that allows for the controlled patterning of functional proteins on the sidewalls of three-dimensional microfabricated chambers. We build microchambers with walls containing gold areas of a defined submicrometer size, to which proteins can be bound specifically, via thiol chemistry. We tested our system by observing the gliding of microtubules along motor-coated microchamber sidewalls and find that introducing an intermediate multilayer of proteins greatly enhances motor protein activity. This combination of spatial confinement and three-dimensional patterning of protein activity opens up new possibilities for the in vitro study of cellular processes, as well as for the development of guided transport mechanisms in nanodevices.
Romet-Lemonne
Guillaume
G
FOM Institute for Atomic and Molecular Physics, Kruislaan 407, 1098 SJ Amsterdam, The Netherlands.
VanDuijn
Martijn
M
Dogterom
Marileen
M
D000327:Adsorption; D032701:Biomimetics / Q000295:instrumentation* / Q000379:methods; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008422:Materials Testing; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization / Q000379:methods; D049329:Nanostructures / Q000032:analysis / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure; D012828:Silicones / Q000737:chemistry
D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D011506:Proteins; D012828:Silicones; C013830:baysilon