Differential beta-arrestin binding of AT1 and AT2 angiotensin receptors.
Agonist stimulation of G protein-coupled receptors causes receptor activation, phosphorylation, beta-arrestin binding and receptor internalization. Angiotensin II (AngII) causes rapid internalization of the AT1 receptors, whereas AngII-bound AT2 receptors do not internalize. Although the activation of the rat AT1A receptor with AngII causes translocation of beta-arrestin2 to the receptor, no association of this molecule with the AT2 receptor can be detected after AngII treatment with confocal microscopy or bioluminescence resonance energy transfer. These data demonstrate that the two subtypes of angiotensin receptors have different mechanisms of regulation.
Turu
Gábor
G
Department of Physiology, Semmelweis University, Faculty of Medicine, H-1444 Budapest, P.O. Box 259, Budapest, Hungary.
Szidonya
László
L
Gáborik
Zsuzsanna
Z
Buday
László
L
Spät
András
A
Clark
Adrian J L
AJ
Hunyady
László
L
D000804:Angiotensin II / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D019390:Arrestins / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D018613:Microscopy, Confocal; D010766:Phosphorylation / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D021381:Protein Transport / Q000187:drug effects / Q000502:physiology; D051381:Rats; D044140:Receptor, Angiotensin, Type 1 / Q000819:agonists* / Q000378:metabolism; D044139:Receptor, Angiotensin, Type 2 / Q000819:agonists* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D014662:Vasoconstrictor Agents / Q000378:metabolism / Q000494:pharmacology*; D000071557:beta-Arrestins
D019390:Arrestins; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D014662:Vasoconstrictor Agents; D000071557:beta-Arrestins; D000804:Angiotensin II