Rapid detection of Aspergillus flavus contamination in peanut with novel delayed luminescence spectra.
Employing a luminescence spectrometer the usefulness of light-induced delayed luminescence (DL) for the detection of aflatoxinB1 (AfB1) contamination in whole peanut was studied. Peanut was artificially contaminated with Aspergillus flavus (7.5 x 10(5)conidia/mL) and incubated for 0-72 h. The DL and fluorescence spectra of contaminated peanut were obtained by a luminescence spectrometer. The correlation between the spectra and contamination levels was established. The DL and fluorescence intensity has a negative correlation with the AfB1 concentration. Our results suggested that the DL technique might be useful for the rapid and noninvasive evaluation of AfB1 contamination levels in whole peanut.
Chen
WenLi
W
College of Life Science, South China Normal University, Guangzhou 510631, China.
Xing
Da
D
Chen
WeiGui
W
D016604:Aflatoxin B1 / Q000032:analysis*; D010367:Arachis / Q000382:microbiology*; D001231:Aspergillus flavus / Q000254:growth & development*; D008163:Luminescent Measurements / Q000379:methods*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors
D016604:Aflatoxin B1