Demineralized bone matrix gelatin as scaffold for osteochondral tissue engineering.
To develop a single-unit osteochondral tissue with demineralized bone matrix gelatin (BMG), rabbit chondrocytes were cultured on demineralized bone matrix gelatin for 6 weeks. The engineered osteochondral tissue was characterized with histology, immunolocalization, TEM, SEM, biochemical assay, and gene expression analysis. About 1.3mm viable neo-cartilage was produced on demineralized BMG. RT-PCR, immunohistochemistry, TEM, biochemical assay, and histology revealed hyaline-like cartilage with zonal layers, intense type II collagen expression, and abundant proteoglycan content formed upon BMG compared with normal cartilage. But hydroxyproline content and type I collagen gene and protein expressions were significantly lower. We consider engineering cartilage tissue with chondrocytes cultured on allogenic demineralized BMG is a good approach for osteochondral tissue engineering.
Li
Xudong
X
Department of Orthopaedic Surgery, University of Virginia School of Medicine, P.O. Box 800374, Charlottesville, VA 22908, USA. xl2n@virginia.edu
Jin
Li
L
Balian
Gary
G
Laurencin
Cato T
CT
Greg Anderson
D
D
D000818:Animals; D001483:Base Sequence; D018775:Bone Demineralization Technique; D001857:Bone Matrix; D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D005780:Gelatin; D008854:Microscopy, Electron; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; D024043:Collagen Type II; D012333:RNA, Messenger; D005780:Gelatin