Apoptosis induction in human lymphoma and leukemia cell lines by transfection via dendrosomes carrying wild-type p53 cDNA.
The efficiency of dendrosomes (novel dendritic spheroidal nanoparticle gene porters) were assessed in transferring wild-type p53 cDNA into two human acute lymphoblastic leukemia cell lines (MOLT-4 and CCRF-CEM) derived from T cells and erythroleukemic cell line K562. Flow cytometric studies showed a 65% and 45% enhancement in apoptosis and necrosis of K562 and CCRF-CEM cells transfected with complex of dendrosomes and wild-type p53 cDNA in comparison to controls. The cytotoxicity studies on T lymphoma cells revealed that dendrosomes have a low cytotoxicity in comparison to lipofectin.
Massumi
Mohammad
M
Institute of Biochemistry and Biophysics, Tehran University, P.O. Box 13145-1384, Tehran, Iran. massumi@ibb.ut.ac.ir
Ziaee
Abed Ali
AA
Sarbolouki
Mohammad Nabi
MN
D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics*; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology*; D049329:Nanostructures; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D018076:DNA, Complementary; D016159:Tumor Suppressor Protein p53