title: Distribution of IgG subclass antibodies specific for Plasmodium falciparum glutamate-rich-protein molecule in sickle cell trait children with asymptomatic infections.
abstract: Polymorphism in the beta-globin gene (hemoglobin S) has been associated with protection against severe forms of malaria. In a cross-sectional study, 180 young Gabonese children with and without sickle cell trait and harboring asymptomatic Plasmodium falciparum infections, were assessed for the responses to recombinant protein containing the conserved region of glutamate-rich protein (GLURP). We reported increased age-dependence of antibody prevalence and levels of total IgG (p<0.0001), IgG1 (p=0.009), and IgG3 (p<0.03) antibodies to GLURP with a cut-off at 5 years of age. Whatever the hemoglobin type, cytophilic antibodies (IgG1 and IgG3) were prevalent, but GLURP-specific IgG4 antibodies were detected at significantly (p<0.05) lower levels in HbAS children. We showed that the distribution of non-cytophilic IgG antibodies differs according to the hemoglobin type and to the malaria antigens tested. This may have possible implication for the clearance of malaria parasites and for protection against severe malaria.
authors.affiliation: Unité de Recherches Médicales, Hôpital Albert Schweitzer, Lambaréné, Gabon.
authors.forename: Davy W
authors.initials: DW
authors.lastname: Matondo Maya
authors.forename: Elie
authors.initials: E
authors.lastname: Mavoungou
authors.forename: Philippe
authors.initials: P
authors.lastname: Deloron
authors.forename: Michael
authors.initials: M
authors.lastname: Theisen
authors.forename: Francine
authors.initials: F
authors.lastname: Ntoumi
mesh_terms: D017677:Age Distribution; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D005260:Female; D005681:Gabon / Q000453:epidemiology; D006441:Hemoglobin A / Q000235:genetics; D006451:Hemoglobin, Sickle / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000145:classification; D007223:Infant; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000235:genetics / Q000276:immunology*; D008297:Male; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D016036:Seroepidemiologic Studies; D012744:Sex Ratio; D012805:Sickle Cell Trait / Q000235:genetics / Q000276:immunology* / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D006451:Hemoglobin, Sickle; D007074:Immunoglobulin G; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C065974:merozoite surface protein 2, Plasmodium; C072492:glutamate-rich protein, Plasmodium; C028672:hemoglobin AA; D006441:Hemoglobin A