Relations between interleukin-1, its receptor antagonist gene polymorphism, and bone mineral density in postmenopausal Korean women.
We investigated the relation between polymorphisms in the interleukin-1(IL-1) and IL-1 receptor antagonist (IL-1ra) gene, and bone mineral density (BMD) in postmenopausal Korean women. The IL-1alpha C(-889)T polymorphism, and IL-1beta C(-511)T polymorphism were examined by restriction fragment length polymorphism, and 86-bp variable number tandem repeat polymorphism in the IL-1ra gene was analyzed by the polymerase chain reaction and electrophoresis in 202 postmenopausal Korean women. Serum osteocalcin, and C-telopeptide of type I collagen were measured using a radioimmunoassay and enzyme-linked immunosorbent assay, respectively. BMD at the lumbar spine and proximal femur was measured by dual-energy X-ray absorptiometer. No significant differences in BMD or in serum bone markers levels were noted across the IL-1alpha or IL-1beta genotype. There were no significant differences in the distribution of IL-1alpha or IL-1beta genotype according to the status of bone mass. BMD in women carrying the A2 allele of the IL-1ra gene was significantly lower than those without this allele, and the A2 allele was more frequent in osteoporotic women than in normal women. These data suggest that IL-1ra gene VNTR polymorphism is a genetic factor that may affect BMD in Korean women.
Kim
Jung Gu
JG
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University Hospital, 28 Yeunkeun-dong, Chongno-gu, Seoul 110-744, Korea. kimjg@plaza.snu.ac.kr
Lim
Kyung Sil
KS
Ku
Seung-Yup
SY
Kim
Seok Hyun
SH
Choi
Young Min
YM
Moon
Shin Yong
SY
D000368:Aged; D044466:Asian People; D015415:Biomarkers / Q000097:blood; D015519:Bone Density / Q000235:genetics* / Q000276:immunology; D001851:Bone Diseases, Metabolic / Q000235:genetics / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000235:genetics*; D008875:Middle Aged; D018598:Minisatellite Repeats; D015663:Osteoporosis, Postmenopausal / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic; D017698:Postmenopause; D012795:Sialoglycoproteins / Q000235:genetics*
D015415:Biomarkers; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D012795:Sialoglycoproteins