title: Transposon mutagenesis in rhizobia which can nodulate both legumes and the nonlegume parasponia.
abstract: The successful use of transposon mutagenesis in a slow-growing Rhizobium permitted the isolation of auxotrophic and symbiotically defective mutants. In these mutants, it was shown that different single-copy Tn5 insertions had occurred. Mutant phenotypes indicated that the control of nitrogenase activity in vitro and in planta is different.
authors.affiliation: Genetics Department, Research School of Biological Sciences, and Botany Department, Australian National University, Canberra City, A.C.T. 2601, and Division of Land Resources Management, CSIRO, Wembley, Western Australia, 6014, Australia.
authors.forename: Y
authors.initials: Y
authors.lastname: Cen
authors.forename: G L
authors.initials: GL
authors.lastname: Bender
authors.forename: M J
authors.initials: MJ
authors.lastname: Trinick
authors.forename: N A
authors.initials: NA
authors.lastname: Morrison
authors.forename: K F
authors.initials: KF
authors.lastname: Scott
authors.forename: P M
authors.initials: PM
authors.lastname: Gresshoff
authors.forename: J
authors.initials: J
authors.lastname: Shine
authors.forename: B G
authors.initials: BG
authors.lastname: Rolfe