Polymorphisms of the human OGG1 gene in laryngeal cancer: implications in radiotherapy response and survival.
The human OGG1 (hOGG1) gene encodes a DNA glycosylase involved in the excision repair of 8-hydroxy-2'-deoxyguanine (8-OH-dG) from oxidatively-damaged DNA. Ser326Cys polymorphism in the hOGG1 gene is involved in the repair of 8-hydroxyguanine in oxidatively damaged DNA, and appears to be related to susceptibility to certain smoking and alcohol-related orolaryngeal cancers.


OBJECTIVE
To analyse if hOGG1 Ser326Cys (exon 7: m6) polymorphism is associated with tumour localization, T, stage and histologic differentiation, and if radiotherapy results were influenced by this polymorphism.


METHODS
Blood samples were obtained before treatment from seventy one patients with laryngeal cancer and screened by a PCR-RFLP method.


RESULTS
Although hOGG1 gene is important in DNA repair mechanisms, no significant association was observed between hOGG1 Ser326Cys (exon 7: m6) polymorphism, tumour characteristics and radiotherapy results.


CONCLUSIONS
So the analysis of this polymorphism is not important for treatment decision in laryngeal cancer patients.
Monteiro
E
E
Portuguese Institute of Oncology, CROP, SA-Porto, Molecular Oncology Unit, Rua António Bernardino Almeida, 4200-072 Porto, Portugal.
Varzim
G
G
Silva
R
R
da Costa
Barreira
B
Lopes
C
C
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy* / Q000601:surgery; D045647:DNA Glycosylases / Q000235:genetics*; D018572:Disease-Free Survival; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy* / Q000601:surgery; D007830:Larynx / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016896:Treatment Outcome
D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human