[Expression of B-glucosidase gene of Sacchromycopsis fibuligera in Pichia pastoris].
A beta-Glucosidase gene (BGL 1) was amplified with PCR from the total DNA of Sacchromycopsis fibuligera, and was linked with pGEM-T vector. After cut down by restriction enzyme from pGEM-T vector, BGL 1 was inserted into the expression vector pPIC9K of Pichia pastoris in reading frame with alpha-factor secreting signal peptide sequence to construct the recombinant plasmid pSHL9K. The recombinant plasmid pSHL9K was transformed into Pichia pastoris GS115 with electroporation. The recombinant Pichia pastoris strains which could efficiently secret recombinant beta-Glucosidase were selected. The optimum temperature of the recombinant beta-Glucosidase was 50degreesC, and the optimum pH was 5.4. The activity of beta-Glucosidase could reach to 47U/mL in the culture medium.
Shao
Jin-hui
JH
State Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences, Beijing.
Zhao
Yun
Y
Mao
Ai-jun
AJ
Zhu
Ya-xin
YX
Dong
Zhi-yang
ZY
Jiang
Ning
N
D010843:Pichia / Q000235:genetics*; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D012442:Saccharomycopsis / Q000201:enzymology* / Q000235:genetics; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
D011994:Recombinant Proteins; D001617:beta-Glucosidase