title: Enzyme-Linked Immunosorbent Assay for Quantitative Detection of Bacillus thuringiensis Crystal Protein.
abstract: Accurate measurement of the toxic protein crystal produced during deep-tank fermentation of Bacillus thuringiensis is critical for optimum process yield. The currently accepted method is a bioassay that requires more time to generate data than to complete the fermentation itself. A noncompetitive enzyme-linked immunosorbent assay has been developed with purified B. thuringiensis crystals to generate rabbit antiserum. This technique gives a quantitative crystal protein value with a colorimetric endpoint for either liquids or powders within 4 h of sampling. Reproducibility of this enzyme-linked immunosorbent assay satisfies criteria for use in a commercial process.
authors.affiliation: Chemical and Agricultural Products Division and Diagnostics Division, Abbott Laboratories, North Chicago, Illinois 60064.
authors.forename: R A
authors.initials: RA
authors.lastname: Smith
authors.forename: J T
authors.initials: JT
authors.lastname: Ulrich