title: Cell Wall Composition of Neurospora crassa Under Conditions of Copper Toxicity.
abstract: The mycelia of Neurospora crassa grown in the presence of high concentrations of copper were blue in color, but only on a medium containing inorganic nitrate and phosphate as the nitrogen and phosphate sources, respectively. The cell wall isolate of the blue mycelia contained large amounts (12%) of copper and higher amounts of chitosan, phosphate, and amino groups, with a 42% decrease in the chitin content. Although all the glucosamine of the cell wall of control cultures could be released within 6 h of hydrolysis with acid, that of the blue mycelium required prolonged hydrolysis for 24 h. On removal of copper, the cell wall of the blue mycelium could quantitatively bind again to copper as well as to zinc. Although zinc binding was fivefold greater, copper alone was preferentially bound from a mixture of the two metal ions. Supplementation of iron along with copper in the culture medium resulted in the disappearance of the blue color of the mycelium and restoration of normal growth and composition of the cell wall, probably by limiting the uptake of copper from the medium. The possibility of the cell wall being a specific site of lesion in copper toxicity in the mold is discussed.
authors.affiliation: Department of Biochemistry, Osmania University, Hyderabad 500 007, India.
authors.forename: C
authors.initials: C
authors.lastname: Subramanyam
authors.forename: G
authors.initials: G
authors.lastname: Venkateswerlu
authors.forename: S L
authors.initials: SL
authors.lastname: Rao