title: Single particle reconstructions of the transferrin-transferrin receptor complex obtained with different specimen preparation techniques.
abstract: The outcome of three-dimensional (3D) reconstructions in single particle electron microscopy (EM) depends on a number of parameters. We have used the well-characterized structure of the transferrin (Tf)-transferrin receptor (TfR) complex to study how specimen preparation techniques influence the outcome of single particle EM reconstructions. The Tf-TfR complex is small (290kDa) and of low symmetry (2-fold). Angular reconstitution from images of vitrified specimens does not reliably converge on the correct structure. Random conical tilt reconstructions from negatively stained specimens are reliable, but show variable degrees of artifacts depending on the negative staining protocol. Alignment of class averages from vitrified specimens to a 3D negative stain reference model using FREALIGN largely eliminated artifacts in the resulting 3D maps, but not completely. Our results stress the need for critical evaluation of structures determined by single particle EM.
authors.affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Cheng
authors.forename: Elmar
authors.initials: E
authors.lastname: Wolf
authors.forename: Mykol
authors.initials: M
authors.lastname: Larvie
authors.forename: Olga
authors.initials: O
authors.lastname: Zak
authors.forename: Philip
authors.initials: P
authors.lastname: Aisen
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Grigorieff
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Harrison
authors.forename: Thomas
authors.initials: T
authors.lastname: Walz
mesh_terms: D002244:Carbon / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D006801:Humans; D021621:Imaging, Three-Dimensional; D008854:Microscopy, Electron / Q000379:methods; D008958:Models, Molecular; D008982:Molybdenum / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry; D011487:Protein Conformation; D011990:Receptors, Transferrin / Q000737:chemistry* / Q000648:ultrastructure; D012984:Software; D013194:Staining and Labeling / Q000379:methods*; D014168:Transferrin / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D009942:Organometallic Compounds; D011990:Receptors, Transferrin; D014168:Transferrin; C000472:uranyl formate; D002244:Carbon; D008982:Molybdenum; D005947:Glucose; C022175:ammonium molybdate; D005990:Glycerol