title: Role of nonadsorbing polymers in bacterial aggregation.
abstract: Bacteria exhibit properties similar to those of nonbiological colloids and can display pairwise attractions when in close proximity. This interaction is governed by the surface chemistry of the cells. We seek to understand bacterial aggregation at the cellular level using Escherichia coli (E. coli) AB1157. Aggregation studies were carried out using 0.5 to 2.5 wt% E. coli AB1157 harvested in different growth phases with varying concentrations of a nonadsorbing polymer, sodium polystyrene sulfonate (SPS). The electrophoretic mobility of E. coli AB1157 in different growth phases was determined using phase-amplitude light scattering. E. coli AB1157 was found to be negatively charged, and the cell surface properties changed in different growth phases. The electrokinetic results correlated well with the different concentrations of nonadsorbing polymer needed to induce depletion aggregation. This shows that a difference in aggregation properties is due to changes in the bacteria electrokinetic properties during their growth.
authors.affiliation: Department of Chemical and Process Engineering, The University of Sheffield, Mappin Street, Sheffield S1 3JD, UK.
authors.forename: K E
authors.initials: KE
authors.lastname: Eboigbodin
authors.forename: J R A
authors.initials: JR
authors.lastname: Newton
authors.forename: A F
authors.initials: AF
authors.lastname: Routh
authors.forename: C A
authors.initials: CA
authors.lastname: Biggs
mesh_terms: D000327:Adsorption; D001422:Bacterial Adhesion; D004586:Electrophoresis; D004926:Escherichia coli / Q000502:physiology*; D011108:Polymers
chemical_list: D011108:Polymers