Giardia lamblia behavior under cytochalasins treatment.
Giardia lamblia, a flagellated protist, is the parasite most commonly found in the intestinal tract of humans and other mammals causing a disease known as giardiasis. This parasite presents several cytoskeletal structures whose major components are microtubules, namely: the ventral adhesive disk, eight flagella axonemes, the median body, and funis. However, the cytoskeletal filamentous structures are poorly understood, and therefore, less studied. In the present work, we used actin-interacting drugs such as cytochalasin B and D to investigate their effects on Giardia ultrastructure. Axenically grown G. lamblia trophozoites were treated with these drugs and analyzed by fluorescence microscopy and scanning and transmission electron microscopy. It was observed that trophozoites became completely misshapen, detached from the glass surface, and failed to complete cell division. The main alterations observed included: (1) disk fragmentation, (2) presence of large vacuoles, (3) alterations in flagella number and flagella internalization, (4) blocked cytokinesis but not the karyokinesis, and (5) presence of membrane undulations and blebs. These findings are discussed.
Corrêa
Gladys
G
Laboratório de Ultraestrutura Celular, Universidade Santa Ursula, Rio de Janeiro, Brazil. marleneben@uol.com.br
Benchimol
Marlene
M
D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D015638:Cytochalasin D / Q000494:pharmacology*; D003572:Cytochalasins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016829:Giardia lamblia / Q000187:drug effects* / Q000648:ultrastructure
D000981:Antiprotozoal Agents; D003572:Cytochalasins; C031218:cytochalasin A; D015638:Cytochalasin D