Comparison of flow cytometry vs. a haematology cell analyser-based method to guide the optimal time-point for peripheral blood stem cell apheresis.
OBJECTIVE
For timing the onset of apheresis, parameters obtained by flow cytometry and by a haematological cell analyser were compared.


METHODS
Haematopoietic cell counts (n = 159) were performed by two different methods; CD34 analyses by flow cytometry, immature myeloid information (IMI) and human progenitor cell counts (HPC) by a haematological cell analyser.


RESULTS
Comparing the IMI total results with CD34+ analyses (n = 159) revealed a correlation of r = 0.46 (P < 0.05). Similar results were obtained for HPC (r = 0.44; P < 0.05).


CONCLUSIONS
The haematology analyser-based method does not allow the precise determination of absolute haematopoietic stem cell numbers and is thus not able to replace flow cytometry for the monitoring of peripheral blood stem cell counts.
Gutensohn
K
K
Institute for Transfusion Medicine, University Hospital Eppendorf, University of Hamburg, Germany.
Magens
M
M
Krüger
W
W
Kröger
N
N
Kühnl
P
P
D001781:Blood Component Removal / Q000295:instrumentation / Q000379:methods; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D006403:Hematologic Tests / Q000295:instrumentation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation / Q000379:methods