title: HPLC determination of phenolic acids and antioxidant activity in concentrated peat extract--a natural immunomodulator.
abstract: The aim of this study was to devise a method for identification and quantification of phenolic acids in concentrated peat extract samples. The simple reversed-phase HPLC method for simultaneous determination of several phenolic acids was developed. The method was validated and it was suitable for the analysis of phenolic acids in peat extracts. This method allowed identifying eight phenolic acids in peat extracts. Phenolic profiles of two samples of peat extract obtained from different medicinal peats were similar, although variations in amounts of individual phenolic acids were observed. Also, slight variations in total phenolic content were detected. The antioxidant activity of peat extracts was evaluated with spectrophotometric ABTS assay. Differences in antioxidant activity were observed for two samples of peat extract produced from different peat varieties. This differences probably reflected phenolic composition of peat extracts.
authors.affiliation: Torf Corporation - Pharmaceutical Factory Ltd., Fabryczna 11, 55-080 Katy Wroclawskie, Poland. m.tarnawski@torf.pl
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Tarnawski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Depta
authors.forename: Danuta
authors.initials: D
authors.lastname: Grejciun
authors.forename: Bogdan
authors.initials: B
authors.lastname: Szelepin
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D062385:Hydroxybenzoates / Q000032:analysis*; D007155:Immunologic Factors / Q000494:pharmacology*; D008956:Models, Chemical; D019800:Phenol / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D012987:Soil; D013053:Spectrophotometry / Q000379:methods*; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000975:Antioxidants; D062385:Hydroxybenzoates; D007155:Immunologic Factors; D010936:Plant Extracts; D012987:Soil; D019800:Phenol; C017616:phenolic acid