title: Heat intolerance: does gene transcription contribute?
abstract: During exertion in the heat, heat-intolerant (HI) subjects have a physiological disability in metabolic heat dissipation. The HI state is either permanent or temporary, depending on whether it stems from transient predisposing factors or inherent thermoregulatory dysfunction. In this investigation, we studied protein levels of heat shock protein (HSP) 70 and HSP72, HSP90, bcl-2xL, glutathione S-transferase-p, heat shock factor-1, TATA-binding protein-associated factor, and NF-kappaB transcripts using Western blot and quantitative RT-PCR, respectively, in lymphocytes of HI and tolerant (T) male volunteers of similar anthropometric features. Measurements were made from blood drawn before, during the heat tolerance test (3.5 mph, 40 degrees C, 40% relative humidity, 2 h), and 1 h after recovery at 24 degrees C. Rectal and skin temperatures, as well as heart rate, were continuously recorded. Of 58 subjects, 7 were identified as HI, with a significantly higher physiological strain index than in the T group (6.3 +/- 0.9 vs. 3.8 +/- 0.6, P < 0.001). The responsiveness of the vasculature to thermal stimuli was decreased in the HI group, as indicated by rectal temperature minus skin temperature. The HSP72 level in the HI group dropped during the recovery session (P < 0.01), whereas that of the T group continued to rise. A significantly increased expression of the transcription factors in the T subjects and significantly decreased expression in the HI group (P < 0.009, 0.013, and 0.005 for heat shock factor-1, NF-kappaB, and TATA-binding protein-associated factor, respectively) points to impaired transcriptional processes in the HI group. Our data suggest that transcriptional malfunction and sluggishness of the vasculature to thermal stimuli are predisposing factors in the HI group.
authors.affiliation: Heller Institute of Medical Research, Sheba Medical Center, Tel Hashomer, Israel.
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Moran
authors.forename: Luba
authors.initials: L
authors.lastname: Eli-Berchoer
authors.forename: Yuval
authors.initials: Y
authors.lastname: Heled
authors.forename: Liran
authors.initials: L
authors.lastname: Mendel
authors.forename: Mara
authors.initials: M
authors.lastname: Schocina
authors.forename: Michal
authors.initials: M
authors.lastname: Horowitz
mesh_terms: D000328:Adult; D001831:Body Temperature; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015444:Exercise / Q000502:physiology; D005786:Gene Expression Regulation; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D000076249:Heat Shock Transcription Factors; D018882:Heat Stress Disorders / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D006358:Hot Temperature; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014666:Vasomotor System / Q000503:physiopathology
chemical_list: D004268:DNA-Binding Proteins; D050884:HSP72 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D016328:NF-kappa B; D012333:RNA, Messenger; D014157:Transcription Factors