title: Friend Leukemogenic Virus-neutralizing Antibody from Mouse Ascitic Fluid.
abstract: Ascitic fluid antibody produced in C57/B1 mice immunized with Friend leukemogenic virus exhibited potent neutralizing activity. In vitro neutralization tests revealed that a mean neutralization index of 3.0 was achieved, and it was shown by sucrose gradient ultracentrifugation that this antibody resembled the 7S type. A mean yield of 6.7 ml of fluid per mouse per weekly paracentesis was obtained over an 8-week period. The ascitic fluid antibody to Friend virus was also active in vivo. Mice given antibody 3, 5, 7, and 9 days after infection with Friend virus did not develop the splenomegaly characteristic of Friend disease.
authors.affiliation: Virus and Disease Modification Section, National Cancer Institute, Bethesda, Maryland 20014.
authors.forename: R W
authors.initials: RW
authors.lastname: March
authors.forename: M A
authors.initials: MA
authors.lastname: Chirigos
authors.forename: W A
authors.initials: WA
authors.lastname: Hook
authors.forename: B L
authors.initials: BL
authors.lastname: Burka