Ion-exchange chromatography followed by ESI-MS for quantitative analysis of sugar monophosphates from glucose catabolism.
The aim of this work is to establish a quantitative method to determine the ratio of [U-(13)C] labeled to unlabeled hexose monophosphates isolated from yeast extracts. This is accomplished by anion exchange chromatography and mobile phase desalting followed by electrospray (ESI) mass spectrometry. We test the method with the analysis of a sample of biological origin. Previously developed analytical techniques are not adequate to accomplish mass spectrometric analysis of these and other small monosaccharide systems because of interference from salt clusters. By lowering the ionic strength of the mobile phase and using a simplified injection system to the mass spectrometer, we were able to obtain data on the relative abundance of the hexose monophosphates.
Walters
James J
JJ
Department of Chemistry, Washington University, One Brookings Drive, Campus Box 1134, 63130, St. Louis, MO, USA.|the Department of Chemistry and Biochemistry, University of Missouri, 63121, St. Louis, MO.
Grayson
Michael A
MA
Department of Chemistry, Washington University, One Brookings Drive, Campus Box 1134, 63130, St. Louis, MO, USA.
Gross
Michael L
ML
Department of Chemistry, Washington University, One Brookings Drive, Campus Box 1134, 63130, St. Louis, MO, USA. mgross@wustl.edu.
Hughes
Maureen
M
Department of Biology, Washington University, St. Louis, Missouri, USA.
Shearer
Georgia
G
Department of Biology, Washington University, St. Louis, Missouri, USA.
Kohl
Daniel H
DH
Department of Biology, Washington University, St. Louis, Missouri, USA.
Bashkin
James
J
Department of Chemistry and Biochemistry, University of Missouri, St. Louis, Missouri, USA.
D002241:Carbohydrates / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D005947:Glucose / Q000378:metabolism*; D007202:Indicators and Reagents; D010710:Phosphates / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D002241:Carbohydrates; D007202:Indicators and Reagents; D010710:Phosphates; D005947:Glucose