title: Preventive effect of vitamin E on iron-induced oxidative damage in rabbit.
abstract: Although iron (Fe), plays an important role in different oxidative steps during the metabolism of the human body, it can cause free radical damage. Iron ions seem to play a major role in initiation and promotion reactions of intracellular lipid peroxidation. The aim of this study was to investigate if vitamin E has a protective effect on oxidative changes in erythrocytes induced by Fe treatment. Thirty male New Zealand white rabbits weighing 1400 +/- 50 g were used in the study. The animals were divided into three groups. The first group (n:10) was given 500 mg/kg iron-dextran through intraperitoneal (ip) injection. The second group was given 500 mg/kg iron-dextran+100 mg/kg vitamin E(ip). The third group constituted the control group and received a saline solution injection. The activities of erythrocyte antioxidant enzymes; Superoxide Dismutase (SOD), Glutatione peroxidase (GSH-Px), Catalase (CAT) and Malondialdehyde (MDA) level, an indicator of lipid peroxidation, were determined. Erythrocyte SOD, GSH-Px and CAT activities were decreased and MDA level was increased in iron-dextran treated animals compared to the control group (P < 0.05). The activities of the three antioxidant enzymes were increased and MDA level was decreased in iron-dextran and vitamin E treated group compared to the control group (P < 0.05). Our data indicate that lipid peroxidation occurs after iron overload in the blood. In the light of our findings, vitamin E administration can prevent the toxic oxidative effects induced by iron-dependent free radical damage in erythrocytes.
authors.affiliation: Department of Physiology, Suleyman Demirel University, School of Medicine, 32260 Isparta, Turkey. ahmetkoyu@tnn.net
authors.forename: Ahmet
authors.initials: A
authors.lastname: Koyu
authors.forename: Fehmi
authors.initials: F
authors.lastname: Ozguner
authors.forename: Sadettin
authors.initials: S
authors.lastname: Caliskan
authors.forename: Halit
authors.initials: H
authors.lastname: Karaca
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D004912:Erythrocytes / Q000201:enzymology; D005609:Free Radicals; D007501:Iron / Q000009:adverse effects*; D007505:Iron-Dextran Complex / Q000009:adverse effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D011817:Rabbits; D012965:Sodium Chloride; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D014810:Vitamin E; D012965:Sodium Chloride; D007505:Iron-Dextran Complex; D007501:Iron