title: Detection of papillomavirus proteins and DNA in paraffin-embedded tissue sections.
abstract: The key events during the papillomavirus life cycle can be mapped in infected tissue samples by antibody detection and in situ hybridization. The ease of immuno-detection varies for different proteins and is dependent on antigen availability. Epitope exposure is sometimes necessary, because the antigen may become masked after formalin fixation and paraffin embedding of the infected tissue. Visualization of both nucleic acid and protein targets can be done simultaneously by combining in situ hybridization and immuno-detection methods.
authors.affiliation: Division of Virology, The National Institute for Medical Research, Mill Hill, London, UK.
authors.forename: Woei Ling
authors.initials: WL
authors.lastname: Peh
authors.forename: John
authors.initials: J
authors.lastname: Doorbar
mesh_terms: D000956:Antigens, Viral / Q000032:analysis; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D017404:In Situ Hybridization, Fluorescence; D007202:Indicators and Reagents; D008856:Microscopy, Fluorescence / Q000379:methods; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D000956:Antigens, Viral; D015342:DNA Probes; D004279:DNA, Viral; D007202:Indicators and Reagents