Glycosylphosphatidylinositol-anchored proteins are required for the transport of detergent-resistant microdomain-associated membrane proteins Tat2p and Fur4p.
In eukaryotic cells many cell surface proteins are attached to the membrane via the glycosylphosphatidylinositol (GPI) moiety. In yeast, GPI also plays important roles in the production of mannoprotein in the cell wall. We previously isolated gwt1 mutants and found that GWT1 is required for inositol acylation in the GPI biosynthetic pathway. In this study we isolated a new gwt1 mutant allele, gwt1-10, that shows not only high temperature sensitivity but also low temperature sensitivity. The gwt1-10 cells show impaired acyltransferase activity and attachment of GPI to proteins even at the permissive temperature. We identified TAT2, which encodes a high affinity tryptophan permease, as a multicopy suppressor of cold sensitivity in gwt1-10 cells. The gwt1-10 cells were also defective in the import of tryptophan, and a lack of tryptophan caused low temperature sensitivity. Microscopic observation revealed that Tat2p is not transported to the plasma membrane but is retained in the endoplasmic reticulum in gwt1-10 cells grown under tryptophan-poor conditions. We found that Tat2p was not associated with detergent-resistant membranes (DRMs), which are required for the recruitment of Tat2p to the plasma membrane. A similar result was obtained for Fur4p, a uracil permease localized in the DRMs of the plasma membrane. These results indicate that GPI-anchored proteins are required for the recruitment of membrane proteins Tat2p and Fur4p to the plasma membrane via DRMs, suggesting that some membrane proteins are redistributed in the cell in response to environmental and nutritional conditions due to an association with DRMs that is dependent on GPI-anchored proteins.
Okamoto
Michiyo
M
Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Ibaraki, Japan.
Yoko-o
Takehiko
T
Umemura
Mariko
M
Nakayama
Ken-Ichi
K
Jigami
Yoshifumi
Y
D026905:Amino Acid Transport Systems / Q000378:metabolism*; D003080:Cold Temperature; D003902:Detergents / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D016153:Genes, Suppressor; D017261:Glycosylphosphatidylinositols / Q000502:physiology*; D021962:Membrane Microdomains / Q000378:metabolism*; D033704:Nucleotide Transport Proteins / Q000378:metabolism*; D021381:Protein Transport; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism
D026905:Amino Acid Transport Systems; D003902:Detergents; C035528:FUR4 protein, S cerevisiae; D017261:Glycosylphosphatidylinositols; D033704:Nucleotide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C429825:TAT2 protein, S cerevisiae; D014364:Tryptophan