title: High-dimensional and large-scale phenotyping of yeast mutants.
abstract: One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.
authors.affiliation: Departments of Integrated Biosciences and Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ohya
authors.forename: Jun
authors.initials: J
authors.lastname: Sese
authors.forename: Masashi
authors.initials: M
authors.lastname: Yukawa
authors.forename: Fumi
authors.initials: F
authors.lastname: Sano
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Nakatani
authors.forename: Taro L
authors.initials: TL
authors.lastname: Saito
authors.forename: Ayaka
authors.initials: A
authors.lastname: Saka
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fukuda
authors.forename: Satoru
authors.initials: S
authors.lastname: Ishihara
authors.forename: Satomi
authors.initials: S
authors.lastname: Oka
authors.forename: Genjiro
authors.initials: G
authors.lastname: Suzuki
authors.forename: Machika
authors.initials: M
authors.lastname: Watanabe
authors.forename: Aiko
authors.initials: A
authors.lastname: Hirata
authors.forename: Miwaka
authors.initials: M
authors.lastname: Ohtani
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sawai
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fraysse
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Latgé
authors.forename: Jean M
authors.initials: JM
authors.lastname: François
authors.forename: Markus
authors.initials: M
authors.lastname: Aebi
authors.forename: Seiji
authors.initials: S
authors.lastname: Tanaka
authors.forename: Sachiko
authors.initials: S
authors.lastname: Muramatsu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Araki
authors.forename: Kintake
authors.initials: K
authors.lastname: Sonoike
authors.forename: Satoru
authors.initials: S
authors.lastname: Nogami
authors.forename: Shinichi
authors.initials: S
authors.lastname: Morishita
mesh_terms: D000199:Actins / Q000737:chemistry; D002467:Cell Nucleus / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005821:Genetic Techniques; D016681:Genome, Fungal; D023281:Genomics; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D009154:Mutation; D016366:Open Reading Frames; D010641:Phenotype; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013997:Time Factors; D015003:Yeasts / Q000235:genetics*
chemical_list: D000199:Actins; D005656:Fungal Proteins