title: A hot pepper gene encoding WRKY transcription factor is induced during hypersensitive response to Tobacco mosaic virus and Xanthomonas campestris.
abstract: Plant WRKY transcription factors were previously implicated in the alteration of gene expression in response to various pathogens. The WRKY proteins constitute a large family of plant transcription factors, whose precise functions have yet to be elucidated. Using a domain-specific differential display procedure, we isolated a WRKY gene, which is rapidly induced during an incompatible interaction between hot pepper and Tobacco mosaic virus (TMV) or Xanthomonas campestris pv . vesicatoria (Xcv). The full-length cDNA of CaWRKY-a (Capsicum annuum WRKY-a) encodes a putative polypeptide of 546 amino acids, containing two WRKY domains with a zinc finger motif. The expression of CaWRKY-a could be rapidly induced by not only chemical elicitor such as salicylic acid (SA) or ethephon but also wounding treatments. The nuclear localization of CaWRKY-a was determined in transient expression system using tobacco BY-2 cells by polyethylene glycol (PEG)-mediated transformation experiment. With oligonucleotide molecules containing the putative W-box sequences as a probe, we confirmed that CaWRKY-a protein had W-box-binding activity. These results suggest that CaWRKY-a might be involved as a transcription factor in plant defense-related signal transduction pathways.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, 1, 5-ga, Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Park
authors.forename: Yun-Chul
authors.initials: YC
authors.lastname: Shin
authors.forename: Boo-Ja
authors.initials: BJ
authors.lastname: Lee
authors.forename: Ki-Jeong
authors.initials: KJ
authors.lastname: Kim
authors.forename: Jeong-Kook
authors.initials: JK
authors.lastname: Kim
authors.forename: Kyung-Hee
authors.initials: KH
authors.lastname: Paek
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002212:Capsicum / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D003001:Cloning, Molecular; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011523:Protoplasts / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D016415:Sequence Alignment; D015398:Signal Transduction; D014027:Tobacco Mosaic Virus / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016959:Xanthomonas campestris / Q000502:physiology*
chemical_list: D005030:Ethylenes; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C036216:ethylene; D020156:Salicylic Acid