The influence of curcumin and manganese complex of curcumin on cadmium-induced oxidative damage and trace elements status in tissues of mice.
Curcumin (diferuoyl methane) from turmeric is a well-known biologically active compound. It has been shown to ameliorate oxidative stress and it is considered to be a potent cancer chemopreventive agent. In our previous study the antioxidative effects of curcumin in cadmium exposed animals were demonstrated. Also manganese exerts protective effects in experimental cadmium intoxication. The present study examined the ability of the manganese complex of curcumin (Mn-curcumin) and curcumin to protect against oxidative damage and changes in trace element status in cadmium-intoxicated male mice. Curcumin or Mn-curcumin were administered at equimolar doses (0.14 mmol/kg b.w.) for 3 days, by gastric gavages, dispersed in methylcellulose. One hour after the last dose of antioxidants, cadmium chloride (33 micromol/kg) was administered subcutaneously. Both curcumin and Mn-curcumin prevented the increase of hepatic lipid peroxidation -- expressed as MDA level, induced by cadmium intoxication and attenuated the Cd-induced decrease of hepatic GSH level. No change in hepatic glutathione peroxidase or catalase activities was found in Cd-exposed mice. A decreased GSH-Px activity was measured in curcumin and Mn-curcumin alone treated mice. Neither curcumin nor Mn-curcumin treatment influenced cadmium distribution in the tissues and did not correct the changes in the balance of essential elements caused by Cd-treatment. The treatment with Mn-curcumin increased the Fe and Mn content in the kidneys of both control and Cd-treated mice and Fe and Cu content in the brain of control mice. In conclusion, regarding the antioxidative action, introducing manganese into the curcumin molecule does not potentiate the studied effects of curcumin.
Eybl
Vladislav
V
Department of Pharmacology and Toxicology, Charles University in Prague, Faculty of Medicine in Pilsen, Czech Republic. vladislav.cybl@lfp.cuni.cz
Kotyzová
Dana
D
Lesetický
Ladislav
L
Bludovská
Monika
M
Koutenský
Jaroslav
J
D000818:Animals; D019256:Cadmium Chloride / Q000493:pharmacokinetics / Q000633:toxicity*; D003474:Curcumin / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008345:Manganese / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D009942:Organometallic Compounds / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D014018:Tissue Distribution; D014131:Trace Elements / Q000493:pharmacokinetics*
D008054:Lipid Peroxides; D009942:Organometallic Compounds; D014131:Trace Elements; D008345:Manganese; D005978:Glutathione; D003474:Curcumin; D019256:Cadmium Chloride