Interactions among the components of the interleukin-10 receptor complex.
We used fluorescence resonance energy transfer previously to show that the interferon-gamma (IFN-gamma) receptor complex is a preformed entity mediated by constitutive interactions between the IFN-gammaR2 and IFN-gammaR1 chains, and that this preassembled entity changes its structure after the treatment of cells with IFN-gamma. We applied this technique to determine the structure of the interleukin-10 (IL-10) receptor complex and whether it undergoes a similar conformational change after treatment of cells with IL-10. We report that, like the IFN-gamma receptor complex, the IL-10 receptor complex is preassembled: constitutive but weaker interactions occur between the IL-10R1 and IL-10R2 chains, and between two IL-10R2 chains. The IL-10 receptor complex undergoes no major conformational changes when cells are treated with cellular or Epstein-Barr viral IL-10. Receptor complex preassembly may be an inherent feature of Class 2 cytokine receptor complexes.
Krause
Christopher D
CD
Department of Molecular Genetics, Microbiology, and Immunology, The University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, NJ 08854, USA.
Mei
Erwen
E
Mirochnitchenko
Olga
O
Lavnikova
Natasha
N
Xie
Junxia
J
Jia
Yiwei
Y
Hochstrasser
Robin M
RM
Pestka
Sidney
S
D000818:Animals; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D031541:Fluorescence Resonance Energy Transfer; D016753:Interleukin-10 / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism*; D017471:Receptors, Interferon / Q000737:chemistry / Q000378:metabolism; D018123:Receptors, Interleukin / Q000737:chemistry / Q000378:metabolism*; D053701:Receptors, Interleukin-10; D000096743:Interferon gamma Receptor
D016753:Interleukin-10; D008565:Membrane Proteins; D021122:Protein Subunits; D017471:Receptors, Interferon; D018123:Receptors, Interleukin; D053701:Receptors, Interleukin-10; D000096743:Interferon gamma Receptor