[New approach to an increase of specificity in determination of antibodies to lipopolysaccharides of Gram-negative bacteria by solid-phase enzyme-linked immunosorbent assay].
A simple and effective method is proposed to increase the sorption of lipopolysaccharides (LPS) of Gram-negative bacteria on the polystyrene surfaces, in which there is no need of preliminary modification of the surface of the solid phase with poly-L-lysine. The method consists in using 0.05 M carbonate buffer (pH 9.6) with 70% of ammonium sulfate at the stage of LPS immobilization. As a result, LPS absorption increases essentially, and the level of non-specific reactions remains low. This makes the usage of additional blocking reagents unnecessary and allows increasing sensitivity and specificity in determination of anti-LPS-antibodies by the solid-phase enzyme-linked immunosorbent assay.
Gordienko
A I
AI
D000907:Antibodies, Bacterial / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000276:immunology*; D007164:Immunosorbents / Q000737:chemistry; D008070:Lipopolysaccharides / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D007164:Immunosorbents; D008070:Lipopolysaccharides