CD40-activated B cells express full lymph node homing triad and induce T-cell chemotaxis: potential as cellular adjuvants.
CD40-activated B cells (CD40-B cells) have previously been introduced as an alternative source of antigen-presenting cells for immunotherapy. CD40-B cells can prime naive and expand memory T cells, and they can be generated in large numbers from very small amounts of peripheral blood derived from healthy individuals or cancer patients alike. Administration of CD40-B cells as a cellular adjuvant would require these cells to migrate toward secondary lymphoid organs and attract T cells in situ, processes guided by specific chemokines and chemokine receptors. Here, we demonstrate that primary, human CD40-B cells express a pattern of adhesion molecules and chemokine receptors necessary for homing to secondary lymphoid organs and have the capacity to migrate to cognate ligands. Furthermore, we show that CD40-B cells express important T-cell attractants and induce strong T-cell chemotaxis. These findings further support the use of CD40-B cells as cellular adjuvant for cancer immunotherapy.
von Bergwelt-Baildon
Michael
M
Molecular Tumor Biology and Tumor Immunology, Clinic I of Internal Medicine, Hematology/Oncology, Kerpener Str 62, 50924 Köln, Germany. joachim.schultze@medizin.uni-koeln.de.
Shimabukuro-Vornhagen
Alexander
A
Popov
Alexey
A
Klein-Gonzalez
Nela
N
Fiore
Francesca
F
Debey
Svenja
S
Draube
Andreas
A
Maecker
Britta
B
Menezes
Isaura
I
Nadler
Lee M
LM
Schultze
Joachim L
JL
D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D015703:Antigens, CD / Q000276:immunology; D019013:CD40 Antigens / Q000276:immunology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory; D007167:Immunotherapy; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology
D000276:Adjuvants, Immunologic; D015703:Antigens, CD; D019013:CD40 Antigens; D012313:RNA