title: [Load of calcium probe Fura -2/AM in Escherichia coli cells].
abstract: The fluorescence characteristics of Fura-2/AM, a kind of calcium probe normally used in analysis of animal cells, were studied in detail in this work. The load of the probe into Escherichia coli cells was elucidated through fluorescence spectra. The combination of Fura-2 with calcium ions inside Escherichiacoli cells was observed, and the transmembrane behaviors of extracellular calcium to Escherichia coli cells were also investigated. The work was aimed at further researches on the calcium-induced horizontal gene transfer in Escherichia coli cells.
authors.affiliation: College of Chemistry and Molecular Sciences, Wuhan University, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Shao
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Wang
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Shen
authors.forename: Rui-xiu
authors.initials: RX
authors.lastname: Cai
mesh_terms: D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D016257:Fura-2; D013050:Spectrometry, Fluorescence
chemical_list: D002118:Calcium; D016257:Fura-2