Effects of Preservatives on Viability of Cryptosporidium parvum Oocysts.
Potassium dichromate and formalin reduced the viability of Cryptosporidium parvum oocysts as assessed by inclusion or exclusion of 4',6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) and excystation. Some formalin-treated oocysts containing dead sporozoites excluded PI; that this fluorogenic assay relies not solely upon exclusion of PI but also upon highlighting of sporozoite nuclei by DAPI is reiterated.
Campbell
A T
AT
Scottish Parasite Diagnostic Laboratory, Stobhill General Hospital, Springburn, Glasgow G21 3UW, and Division of Environmental Health, University of Strathclyde, Glasgow G1 1XQ, Scotland.
Robertson
L J
LJ
Smith
H V
HV