Expressed sequence tags analysis of Blattella germanica.
Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.
Chung
Hyang Suk
HS
Department of Otorhinolaryngology, Kosin University College of Medicine, Busan, Korea.
Yu
Tai Hyun
TH
Kim
Bong Jin
BJ
Kim
Sun Mi
SM
Kim
Joo Yeong
JY
Yu
Hak Sun
HS
Jeong
Hae Jin
HJ
Ock
Mee Sun
MS
D000818:Animals; D001483:Base Sequence; D020048:Blattellidae / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment