[Expression and function of receptors for advanced glycation end products in bovine corneal endothelial cells].
Corneal endothelial cell loss is a change that occurs with age, but its mechanism is still unclear. We postulated that interaction between advanced glycation end product(AGE) and its receptors is implicated in the corneal endothelial cell loss with age. We investigated the expression of AGE receptors: receptors for AGE(RAGE) and galectin-3 in bovine corneal endothelial cells by reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemistry. In addition, we investigated the effect of AGE on the cultured corneal endothelial cells. Expression of RAGE and galectin-3 was detected in bovine corneal endothelial cells. Galectin-3 was important in the internalization of AGE. In contrast, RAGE was important in the generation of reactive oxygen species and induction of apoptosis. Based on these data, the interaction of AGE in aqueous humor and AGE receptors expressed on the corneal endothelial cells was speculated to have a role in the corneal endothelial cell loss with age.
Kaji
Yuichi
Y
University of Tsukuba, Institute of Clinical Medicine, Department of Ophthalmology, 1-1-1 Tennoudai, Tsukuba 305-8575, Japan. kajiyuichi@hotmail.com
D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology* / Q000502:physiology; D042783:Endothelial Cells / Q000378:metabolism*; D037502:Galectin 3 / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D008969:Molecular Sequence Data; D017382:Reactive Oxygen Species / Q000378:metabolism; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000378:metabolism*
D037502:Galectin 3; D017127:Glycation End Products, Advanced; D017382:Reactive Oxygen Species; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic