title: [Synthesis, characterization and evaluation of octadecyl ester-bonded monolithic column].
abstract: An ester-bonded monolithic silica column for reversed-phase high performance liquid chromatography was synthesized by reacting stearic acid with gamma-glycidoxypropyltrimethoxysilane and coupling the intermediate product onto monolithic silica column. Characterization of the prepared column was carried out with Fourier transformation infrared spectrometry and elemental analysis. The chromatographic properties of the packing were evaluated by using benzene, biphenyl, and anthracene as samples and methanol-water as binary mobile phase. The column back-pressure and separation effect were also evaluated for fast separation. The results show that the stationary phase has satisfactory chromatographic properties and resists hydrolysis between pH = 2 - 8, and it can be used for the fast-separation of solutes efficiently.
authors.affiliation: College of Pharmacy, Hebei University, Baoding 071002, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.forename: Gengliang
authors.initials: G
authors.lastname: Yang
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Junfa
authors.initials: J
authors.lastname: Yin
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Li
authors.forename: Wenhui
authors.initials: W
authors.lastname: Gao
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000873:Anthracenes / Q000032:analysis; D001554:Benzene / Q000032:analysis; D001713:Biphenyl Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D004952:Esters / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D012821:Silanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013229:Stearic Acids / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000873:Anthracenes; D001554:Benzene; D001713:Biphenyl Compounds; D004952:Esters; D000432:Methanol; D012821:Silanes; D013229:Stearic Acids; D014867:Water; C039462:gamma-glycidoxypropyltrimethoxysilane; C010574:biphenyl; C031183:stearic acid; C034020:anthracene