Enzymatic preparation of maltohexaose, maltoheptaose, and maltooctaose by the preferential cyclomaltooligosaccharide (cyclodextrin) ring-opening reaction of Pyrococcus furiosus thermostable amylase.
Specific-length maltooligosaccharides, particularly maltohexaose, maltoheptaose, and maltooctaose, were prepared from cyclomaltooligosaccharides (cyclodextrins, CDs) by the preferential cyclodextrin ring-opening reaction of an amylolytic enzyme from Pyrococcus furiosus. The enzyme primarily produces maltohexaose, maltoheptaose, and maltooctaose by hydrolyzing alpha-, beta-, and gamma-CD, respectively. This study aims to develop a high-efficiency synthesis of specific maltooligosaccharides at high-purity. [formula: see text]
Yang
Sung-Jae
SJ
Center for Agricultural Biomaterials and Department of Food and Animal Biotechnology, School of Agricultural Biotechnology, Seoul National University, Shillim-dong, Kwanak-gu, Seoul 151-921, Republic of Korea.
Lee
Hee-Seob
HS
Kim
Jung-Woo
JW
Lee
Myoung-Hee
MH
Auh
Joong-Hyuck
JH
Lee
Byong-Hoon
BH
Park
Kwan-Hwa
KH
D000681:Amylases / Q000737:chemistry / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003505:Cyclodextrins / Q000378:metabolism*; D004795:Enzyme Stability; D005936:Glucans / Q000302:isolation & purification / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D009844:Oligosaccharides / Q000302:isolation & purification / Q000378:metabolism*; D020027:Pyrococcus furiosus / Q000201:enzymology*
D003505:Cyclodextrins; D005936:Glucans; D009844:Oligosaccharides; C016549:maltohexaose; C031146:maltoheptaose; C101183:maltooctaose; D000681:Amylases