SC5 mAb represents a unique tool for the detection of extracellular vimentin as a specific marker of Sezary cells.
Circulating malignant Sézary lymphocytes result from a clonal proliferation of memory/activated CD4(+)CD45RO(+) T lymphocytes primarily involving the skin. Recently, the CD158k/KIR3DL2 cell surface receptor has been identified to phenotypically characterize these cells. We previously described a mAb termed SC5 that identifies an unknown early activation cell membrane molecule. It is expressed selectively by T lymphocytes isolated from healthy individuals upon activation, and by circulating Sézary syndrome lymphocytes. In addition, we found that SC5 mAb was reactive with all resting T lymphocytes once permeabilized, indicating that SC5 mAb-reactive molecule might present distinct cellular localization according to the T cell activation status. In this study, we show for the first time that SC5 mAb recognizes the intermediate filament protein vimentin when exported to the extracellular side of the plasma membrane of viable Sézary malignant cells. We demonstrate that SC5 mAb is unique as it reacts with both viable malignant lymphocytes and apoptotic T cells. As vimentin is also detected rapidly at the cell membrane surface after normal T lymphocyte activation, it suggests that its extracellular detection on Sézary cells could be a consequence of their constitutive activation status. Finally, as a probable outcome of vimentin cell surface expression, autoantibodies against vimentin were found in the sera of Sézary syndrome patients.
Huet
Delphine
D
Institut National de la Santé et de la Recherche Médicale Unité 659, University of Paris XII, Medical School of Créteil, Henri Mondor Hospital, Department of Dermatology, Créteil, France.
Bagot
Martine
M
Loyaux
Denis
D
Capdevielle
Joël
J
Conraux
Laurence
L
Ferrara
Pascual
P
Bensussan
Armand
A
Marie-Cardine
Anne
A
D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001666:Binding Sites, Antibody; D014408:Biomarkers, Tumor / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D018613:Microscopy, Confocal; D011956:Receptors, Cell Surface / Q000276:immunology*; D012751:Sezary Syndrome / Q000175:diagnosis* / Q000276:immunology; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014746:Vimentin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D014408:Biomarkers, Tumor; D011956:Receptors, Cell Surface; C429763:SC5 receptor, human; D014746:Vimentin