title: Analysis of free amino acids in microbially colonized sandstone by precolumn phenyl isothiocyanate derivatization and high-performance liquid chromatography.
abstract: A fast, sensitive method for extraction and analysis of soluble free amino acids from microbially colonized sandstone is described. After precolumn phenyl isothiocyanate (PITC) derivatization, the PITC-amino acids were identified and quantified by reversed-phase high-performance liquid chromatography. This kind of analysis could be used to elucidate the role and function of amino acids in the nutrition of epi- and endolithic microorganisms active in biological weathering processes.
authors.affiliation: Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität, D-2300 Kiel, Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Siebert
authors.forename: R J
authors.initials: RJ
authors.lastname: Palmer
authors.forename: P
authors.initials: P
authors.lastname: Hirsch