A differential medium for the enumeration of homofermentative and heterofermentative lactic Acid bacteria.
A medium was developed for the differential enumeration of homofermentative and heterofermentative lactic acid bacteria. Essential components of the medium included fructose (14 mM), KH(2)PO(4) (18 mM), bromcresol green (as a pH indicator), and other nutrients to support growth. In agar medium, homofermentative colonies were blue to green, while heterofermentative colonies remained white. A total of 21 Lactobacillus, Pediococcus, Leuconostoc, and Streptococcus species were correctly classified with the medium.
McDonald
L C
LC
Food Fermentation Laboratory, Agricultural Research Service, U.S. Department of Agriculture, and Department of Food Science, North Carolina Agricultural Research Service, North Carolina State University, Raleigh, North Carolina 27695-7624.
McFeeters
R F
RF
Daeschel
M A
MA
Fleming
H P
HP