title: PII structure in the model peptides for unfolded proteins: studies on ubiquitin fragments and several alanine-rich peptides containing QQQ, SSS, FFF, and VVV.
abstract: A great deal of attention has been paid lately to the structures in unfolded proteins due to the recent discovery of many biologically functional but natively unfolded proteins and the far-reaching implications of order in unfolded states for protein folding. Recently, studies on oligo-Ala, oligo-Lys, oligo-Asp, and oligo-Glu, as well as oligo-Pro, have indicated that the left-handed polyproline II (PII) is the major local structure in these short peptides. Here, we show by NMR and CD studies that ubiquitin fragments, model unfolded peptides composed of nonrepeating amino acids, and four alanine-rich peptides containing QQQ, SSS, FFF, and VVV sequences are all present in aqueous solution predominantly in the extended PII or beta conformation. The results from this and related studies indicate that PII might be a major backbone conformation in unfolded proteins. The presence of defined local backbone structure in unfolded proteins is inconsistent with predictions from random coil models.
authors.affiliation: Department of Chemistry, New York University, New York, NY 10003, USA.
authors.forename: Zhengshuang
authors.initials: Z
authors.lastname: Shi
authors.forename: Kang
authors.initials: K
authors.lastname: Chen
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
authors.forename: Tobin R
authors.initials: TR
authors.lastname: Sosnick
authors.forename: Neville R
authors.initials: NR
authors.lastname: Kallenbach
mesh_terms: D000409:Alanine / Q000737:chemistry*; D000595:Amino Acid Sequence; D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D025801:Ubiquitin / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D010455:Peptides; D025801:Ubiquitin; C011083:polyproline; D000409:Alanine