Evaluation of the biofilm-forming ability and genetic typing for clinical isolates of Pseudomonas aeruginosa by enterobacterial repetitive intergenic consensus-based PCR.
Biofilm formation is an important phenotype associated with chronic Pseudomonas aeruginosa infections. In the present study, a total of 48 P. aeruginosa strains isolated from clinical specimens were examined for their biofilm-forming ability using a microtiter plate method. The different biofilm-forming abilities were demonstrated among the strains; however, most strains formed a larger biofilm than strain PAO1, a reference strain. The genetic typing was also carried out by enterobacterial repetitive intergenic consensus-based polymerase chain reaction. Although they were divided into five groups (A to E), most of the strains showing the higher biofilm-forming ability were found to be in groups D and E, suggesting a significant relationship between the biofilm-forming ability and the genetic group.
Yang
Weiqing
W
Department of Microbiology, West China School of Basic and Forensic Medical Sciences, Sichuan University, 610041 Chengdu, China.
Shi
Lei
L
Jia
Wen-xiang
WX
Yin
Xiaolin
X
Su
Jian-yu
JY
Kou
Yali
Y
Yi
Xie
X
Shinoda
Sumio
S
Miyoshi
Shin-ichi
S
D015373:Bacterial Typing Techniques; D018441:Biofilms / Q000254:growth & development*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics*; D006761:Hospitals; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000502:physiology*; D012091:Repetitive Sequences, Nucleic Acid
D004269:DNA, Bacterial