title: Expression of sFRP-4 and beta-catenin in human colorectal carcinoma.
abstract: Alterations to the Wnt signalling pathway occur in the majority of colorectal cancers and result in abnormal accumulation of beta-catenin. The secreted frizzled related proteins (sFRPs) are antagonists that bind Wnt and inhibit signalling along this pathway. We investigated expression of the sFRP family member, sFRP-4, and beta-catenin in 1,044 human colorectal carcinomas using tissue microarrays and immunohistochemistry. Both proteins showed markedly increased expression levels in tumors compared to normal mucosa, but no significant associations with pathological features or with patient outcome. sFRP-4 was co-expressed with beta-catenin, p53, and COX-2, while the absence of beta-catenin expression was strongly associated with loss of expression of the MLH1 mismatch repair gene. In contrast to other sFRP family members, sFRP-4 expression appears to be upregulated in colorectal carcinoma.
authors.affiliation: Department of Gastroenterological Surgery and Institute of Digestive System Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Feng Han
authors.forename: Wenying
authors.initials: W
authors.lastname: Zhao
authors.forename: Jacky
authors.initials: J
authors.lastname: Bentel
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Shearwood
authors.forename: Nik
authors.initials: N
authors.lastname: Zeps
authors.forename: David
authors.initials: D
authors.lastname: Joseph
authors.forename: Barry
authors.initials: B
authors.lastname: Iacopetta
authors.forename: Arun
authors.initials: A
authors.lastname: Dharmarajan
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D020137:Base Pair Mismatch; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002352:Carrier Proteins / Q000096:biosynthesis; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004260:DNA Repair; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D000070957:MutL Protein Homolog 1; D009687:Nuclear Proteins / Q000096:biosynthesis; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D015854:Up-Regulation; D051176:beta Catenin / Q000096:biosynthesis*; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D000070957:MutL Protein Homolog 1; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D051176:beta Catenin; C501486:MLH1 protein, human; C118723:SFRP4 protein, human; D000094742:Secreted Frizzled-Related Proteins