title: The metabolic profiles of transgenic cucumber lines vary with different chromosomal locations of the transgene.
abstract: The metabolic profiles of five transgenic cucumber lines were compared taking into consideration their transgene integration sites. The plants analyzed were homozygous and contained transgenes integrated in a single locus on chromosomes I, II, III or IV. The transgenes were preferentially located in the euchromatic regions. Each of these locations possessed a specific metabolic profile. The number of altered compounds in the transgenic lines varied between 9 and 23 of the 47 metabolites identified. These alterations seem to be specific for each independent transgene integration. However, some changes are common: a decrease in the levels of phenylalanine, aspartate, ethanolamine and pipecolate, and an increase in the level of benzoic acid. The observed effects of transgene introduction are discussed in this paper.
authors.affiliation: Faculty of Human Life Science, Hiroshima Jogakuin University, Japan.
authors.forename: Norikazu
authors.initials: N
authors.lastname: Tagashira
authors.forename: Wojciech
authors.initials: W
authors.lastname: Plader
authors.forename: Marcin
authors.initials: M
authors.lastname: Filipecki
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Yin
authors.forename: Anira
authors.initials: A
authors.lastname: Wiśniewska
authors.forename: Paweł
authors.initials: P
authors.lastname: Gaj
authors.forename: Maria
authors.initials: M
authors.lastname: Szwacka
authors.forename: Oliver
authors.initials: O
authors.lastname: Fiehn
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Hoshi
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kondo
authors.forename: Stefan
authors.initials: S
authors.lastname: Malepszy
mesh_terms: D002871:Chromosome Banding; D032461:Chromosomes, Plant / Q000235:genetics*; D018553:Cucumis sativus / Q000235:genetics* / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified; D019076:Transgenes / Q000235:genetics*