Capucin: a novel striatal marker down-regulated in rodent models of Huntington disease.
In an initial study, we compared quantitative transcriptome data across mouse brain territories using the serial analysis of gene expression method. Among the novel regional markers that we discovered, we focused on a striatum-enriched transcript with no available experimental cDNA sequence. Here, we report its cloning, gene structure, and detailed distribution in mouse brain. Quantitative RT-PCR and in situ hybridization demonstrated predominant expression in dorsolateral striatum. We therefore named it capucin for caudate-and putamen-enriched sequence. Mouse capucin is a 237-amino-acid protein, without any registered ortholog in mammalian species. It contains no recognizable motif other than two predicted carboxy-terminal transmembrane domains. When expressed in fusion with a fluorescent protein, it localized to the Golgi apparatus in two mammalian cell lines. Interestingly, we observed a significant down-regulation of capucin mRNA levels in two rodent models of Huntington disease, indicating a possible contribution to the pathogenesis of this disorder.
de Chaldée
M
M
Laboratoire de Physiogénomique, Service de Biochimie et de Génétique Moléculaire, Commissariat à l'Energie Atomique, Saclay, 91191 Gif-sur-Yvette Cedex, France. chaldee@dsvidf.cea.fr
Brochier
C
C
Van de Vel
A
A
Caudy
N
N
Luthi-Carter
R
R
Gaillard
M C
MC
Elalouf
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers; D003001:Cloning, Molecular; D003342:Corpus Striatum / Q000378:metabolism*; D018076:DNA, Complementary; D004195:Disease Models, Animal; D015536:Down-Regulation; D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism
D015415:Biomarkers; D018076:DNA, Complementary; D008565:Membrane Proteins; C511357:Syndig1l protein, mouse