title: Differential beta-adrenoceptor expression induced by nerve growth factor infusion into the canine right and left stellate ganglia.
abstract: BACKGROUND
  Nerve growth factor (NGF) infusion into the right stellate ganglion (RSG) is antiarrhythmic, while NGF infusion into the left stellate ganglion (LSG) is proarrhythmic in dogs with myocardial infarction (MI) and complete atrioventricular block (CAVB). This functional asymmetry suggests differential neural remodeling.
  
  
  OBJECTIVE
  To test the hypothesis that NGF infusion into the RSG and the LSG can lead to differential beta-adrenoceptor (beta-AR) expression in dogs with MI and CAVB.
  
  
  RESULTS
  We performed immunostaining to quantify beta(1)-AR and beta(3)-AR immunoreactivity in six dogs with MI and CAVB, nine dogs with MI, CAVB, and NGF infusion to the LSG, six dogs with MI, CAVB, and NGF infusion to the RSG, and six normal dogs. There was significantly increased beta(3)-AR immunoreactivity in dogs with NGF infusion into the LSG and significantly decreased beta(3)-AR immunoreactivity in dogs with NGF infusion into the RSG compared with controls and with the MI and CAVB group. There were no significant differences in beta(1)-AR immunoreactivity among these four groups. To determine protein and mRNA expression of beta-ARs, we created MI and CAVB and infused NGF into the LSG in six additional dogs. The noninfarcted left ventricle free wall was harvested 1 week later. The protein level and receptor density of beta(3)-AR (but not beta(1)- or beta(2)-AR) significantly increased in these six dogs compared with normal controls.
  
  
  CONCLUSIONS
  We conclude that NGF infusion into the RSG and the LSG in dogs with MI and CAVB induced differential beta(3)-AR expression in the left ventricular myocardium.
authors.affiliation: Division of Cardiology, Department of Medicine, University of Southern California, USA.
authors.forename: Shengmei
authors.initials: S
authors.lastname: Zhou
authors.forename: Offir
authors.initials: O
authors.lastname: Paz
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Cao
authors.forename: Kamlesh
authors.initials: K
authors.lastname: Asotra
authors.forename: Ning-Ning
authors.initials: NN
authors.lastname: Chai
authors.forename: Charles
authors.initials: C
authors.lastname: Wang
authors.forename: Lan S
authors.initials: LS
authors.lastname: Chen
authors.forename: Michael C
authors.initials: MC
authors.lastname: Fishbein
authors.forename: Behrooz
authors.initials: B
authors.lastname: Sharifi
authors.forename: Peng-Sheng
authors.initials: PS
authors.lastname: Chen
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D006327:Heart Block / Q000150:complications / Q000378:metabolism; D006352:Heart Ventricles / Q000378:metabolism; D015918:Infusion Pumps, Implantable; D009203:Myocardial Infarction / Q000150:complications / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D020932:Nerve Growth Factor / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D018342:Receptors, Adrenergic, beta-1 / Q000378:metabolism*; D022702:Receptors, Adrenergic, beta-3 / Q000378:metabolism*; D013233:Stellate Ganglion / Q000187:drug effects*; D017180:Tachycardia, Ventricular / Q000209:etiology; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D018342:Receptors, Adrenergic, beta-1; D022702:Receptors, Adrenergic, beta-3; D020932:Nerve Growth Factor