Vaccination of chicken embryos with escape mutants of La Sota Newcastle disease virus induces a protective immune response.
To reduce the embryonic pathogenicity of Newcastle disease virus (NDV), escape mutants of the La Sota strain were produced with selected monoclonal antibodies. Immunoselection resulted in the elimination of an epitope by single amino acid substitution (F and HN molecule) or in a conformational change (HN molecule). The embryonic pathogenicity of these escape mutants was reduced and their dose was optimised for in ovo vaccination. Because antibody responses and protection of in ovo vaccinated chicks were similar to controls vaccinated at hatch with the La Sota strain, immunoselection appears a valuable technique to produce attenuated NDV strains, which are candidate in ovo vaccines.
Mast
Jan
J
Veterinary and Agrochemical Research Centre, Groeselenberg 99, B-1180 Brussels, Belgium. jamas@var.fgov.be
Nanbru
Cécile
C
Decaesstecker
Mireille
M
Lambrecht
Bénédicte
B
Couvreur
Bernard
B
Meulemans
Guy
G
van den Berg
Thierry
T
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002642:Chick Embryo / Q000276:immunology* / Q000821:virology; D002645:Chickens / Q000276:immunology*; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005814:Genes, Viral; D015707:HN Protein / Q000032:analysis / Q000235:genetics / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008969:Molecular Sequence Data; D009154:Mutation; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D017422:Sequence Analysis, DNA; D013047:Specific Pathogen-Free Organisms; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral; D000939:Epitopes; D015707:HN Protein; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014613:Vaccines, Attenuated; D014760:Viral Fusion Proteins; D014765:Viral Vaccines