title: Thermostability of Fumonisin B(1), a Mycotoxin from Fusarium moniliforme, in Corn.
abstract: Fumonisin B(1) (FB(1)) is a mycotoxin from Fusarium moniliforme that is frequently associated with corn. Thermal treatments are used in many processes concerning this cereal and its derivatives. The thermostability of this toxin in dry contaminated corn, resulting from F. moniliforme culture, was studied in different time-temperature combinations. FB(1) was quantified by instrumentalized thin-layer chromatography after a two-step sequential development and postchromatographic derivatization by p-anisaldehyde. The identity of FB(1) in extracts, before and after heat treatments, was confirmed by high-pressure liquid chromatography. For each temperature, the natural logarithm of the ratio of resulting FB(1) on initial content (In C/C(0)) is linearly correlated to exposure time. The calculated half-lives (L(50)), corresponding to the 50% value, were 10 min, 38 min, 175 min, and 8 h at 150, 125, 100, and 75 degrees C, respectively. There is a linear relationship between calculated L(50)s on a logarithmic scale and temperature. Therefore FB(1) is not significantly destroyed by the main drying processes of corn or thermal treatments used for its derivatives. Other associated means are required for detoxification.
authors.affiliation: Laboratoire de Pharmacologie-Toxicologie, Institut National de la Recherche Agronomique, B.P. 3, 31931 Toulouse Cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Dupuy
authors.forename: P
authors.initials: P
authors.lastname: Le Bars
authors.forename: H
authors.initials: H
authors.lastname: Boudra
authors.forename: J
authors.initials: J
authors.lastname: Le Bars