title: HomoMINT: an inferred human network based on orthology mapping of protein interactions discovered in model organisms.
abstract: BACKGROUND
  The application of high throughput approaches to the identification of protein interactions has offered for the first time a glimpse of the global interactome of some model organisms. Until now, however, such genome-wide approaches have not been applied to the human proteome.
  
  
  RESULTS
  In order to fill this gap we have assembled an inferred human protein interaction network where interactions discovered in model organisms are mapped onto the corresponding human orthologs. In addition to a stringent assignment to orthology classes based on the InParanoid algorithm, we have implemented a string matching algorithm to filter out orthology assignments of proteins whose global domain organization is not conserved. Finally, we have assessed the accuracy of our own, and related, inferred networks by benchmarking them against i) an assembled experimental interactome, ii) a network derived by mining of the scientific literature and iii) by measuring the enrichment of interacting protein pairs sharing common Gene Ontology annotation.
  
  
  CONCLUSIONS
  The resulting networks are named HomoMINT and HomoMINT_filtered, the latter being based on the orthology table filtered by the domain architecture matching algorithm. They contains 9749 and 5203 interactions respectively and can be analyzed and viewed in the context of the experimentally verified interactions between human proteins stored in the MINT database. HomoMINT is constantly updated to take into account the growing information in the MINT database.
authors.affiliation: Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy. maria@cbm.bio.uniroma2.it
authors.forename: Maria
authors.initials: M
authors.lastname: Persico
authors.forename: Arnaud
authors.initials: A
authors.lastname: Ceol
authors.forename: Caius
authors.initials: C
authors.lastname: Gavrila
authors.forename: Robert
authors.initials: R
authors.lastname: Hoffmann
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Florio
authors.forename: Gianni
authors.initials: G
authors.lastname: Cesareni
mesh_terms: D000465:Algorithms; D019295:Computational Biology / Q000379:methods*; D016678:Genome; D006801:Humans; D016247:Information Storage and Retrieval / Q000379:methods*; D020407:Internet; D015233:Models, Statistical; D010363:Pattern Recognition, Automated; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D020543:Proteome / Q000145:classification; D016415:Sequence Alignment / Q000379:methods; D020539:Sequence Analysis, Protein / Q000379:methods*; D012984:Software
chemical_list: D011506:Proteins; D020543:Proteome