Isolation, enumeration, and maintenance of rumen anaerobic fungi in roll tubes.
Anaerobic phycomycetous fungi have been isolated from rumen fluid by using the Hungate roll tube technique. Cultures on solid substrates in roll tubes can be stored for long periods, and fungal zoospores can be enumerated in roll tubes.
Joblin
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Applied Biochemistry Division, Department of Scientific and Industrial Research, Palmerston North, New Zealand.