Inhibitory effects of eutigosides isolated from Eurya emarginata on the inflammatory mediators in RAW264.7 cells.
The anti-inflammatory activity of Eurya emarginata (Thumb) Makino, of which leaves have been traditionally used to treat ulcers or diuretic in Jeju Island, has been investigated in the present study. Through the phytochemical study from the methanol extract of E. emaginiata, eutigosides B and C were isolated as the active components. Sseveral inflammatory markers including TNF-alpha, IL-1beta, IL-6, NO, iNOS, and COX-2 were examined. Eutigosides B and C potentially inhibited production of pro-inflammatory cytokines (IL-6 and TNF-alpha) in a dose-dependent manner. Additionally, the intracellular contents of iNOS protein were markedly decreased after treatment with eutigosides B and C. The inhibition of iNOS activity was correlated with the decrease in nitrite levels. These results suggest that eutigoside B and C from E. emarginata may have anti-inflammatory activity through the inhibition of pro-inflammatory cytokines (TNF-alpha and IL-6), iNOS and COX-2.
Park
Soo-Yeong
SY
Biodiversity Research Institute, Jeju HiDL, Ara 1-dong, Jeju 690-756, Korea.
Lee
Hye-Ja
HJ
Yoon
Weon-Jong
WJ
Kang
Gyoung-Jin
GJ
Moon
Ji-Young
JY
Lee
Nam-Ho
NH
Kim
Se-Jae
SJ
Kang
Hee-Kyoung
HK
Yoo
Eun-Sook
ES
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018749:RNA, Plant / Q000737:chemistry / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D028242:Theaceae / Q000737:chemistry*
D003374:Coumarins; D052246:Cyclooxygenase 2 Inhibitors; D004791:Enzyme Inhibitors; D005960:Glucosides; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D010636:Phenols; D018749:RNA, Plant; C505498:eutigoside A; C505499:eutigoside C; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II