Antibodies against Leishmania cross-react with Crithidia luciliae: indirect immunofluorescence and Dot-ELISA study in dogs.
Leismaniasis is zoonotic parasitic disease distributed in many areas of Mediterranean basin. Because of its pathogenicity and difficulty to grow in vitro, we used Leishmania infantum as primary and Crithidia luciliae as secondary source of antigen. We compared 100 canine sera by indirect immunofluorescence and Dot-ELISA. Both atigens showed same results.
Martinkovic
Franjo
F
Department of Parasitology and Parasitic Diseases, Veterinary Faculty, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia. q_franjo@yahoo.com
Marinculic
Albert
A
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology; D003421:Crithidia / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005188:False Negative Reactions; D005189:False Positive Reactions; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D015151:Immunoblotting; D018314:Leishmania infantum / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D010273:Parasitic Diseases, Animal / Q000175:diagnosis* / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan