Minimal Requirements for Exponential Growth of Lactococcus lactis.
A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis, with a specific growth rate in batch culture of mu = 0.3 h. With 19 amino acids added, the growth rate increased to mu = 0.7 h and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.
Jensen
P R
PR
Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby, Denmark, and Division of Molecular Biology, Netherlands Cancer Institute H5, NL-1066-CX, Amsterdam, The Netherlands.
Hammer
K
K