Safe and efficient collection of cytokine-mobilized peripheral blood cells from cynomolgus monkeys (Macaca fascicularis) with human newborn-equivalent body weights.
Hematopoietic stem cells in bone marrow can be mobilized into peripheral blood by cytokine administration. Cytokine-mobilized peripheral blood stem cells are of great use in clinical applications. We previously established a modified procedure for the collection of cytokine-mobilized peripheral blood cells from rhesus monkeys (Macaca mulata) using a commercially available apparatus originally developed for human subjects. In this study, we examined the efficacy and safety of this method with even smaller macaques, cynomolgus monkeys (Macaca fascicularis), which are equivalent to human newborns in body weight (mean = 3.3 kg). Using the manufacturer's unmodified protocol (n=6), one monkey died of cardiac failure and three developed severe anemia. In contrast, using our modified procedure (n=6), no such complication was observed in any animal. In addition, the harvested nuclear cell, mononuclear cell and CD34(+) cell counts were significantly higher with the modified method. The modified method should allow safe and efficient collection of cytokine-mobilized peripheral blood cells from non-human primates as small as human newborns in a non-invasive manner.
Ageyama
Naohide
N
Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Ibaraki, Japan.
Hanazono
Yutaka
Y
Shibata
Hiroaki
H
Ono
Fumiko
F
Ogawa
Hiromi
H
Nagashima
Takeyuki
T
Ueda
Yasuji
Y
Yoshikawa
Yasuhiro
Y
Hasegawa
Mamoru
M
Ozawa
Keiya
K
Terao
Keiji
K
D000740:Anemia / Q000139:chemically induced; D000818:Animals; D001772:Blood Cell Count; D001854:Bone Marrow Cells / Q000187:drug effects*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006412:Hematopoietic Stem Cells; D007937:Leukapheresis / Q000295:instrumentation / Q000379:methods*; D008252:Macaca fascicularis / Q000502:physiology*; D008297:Male; D019089:Stem Cell Factor / Q000494:pharmacology*
D019089:Stem Cell Factor; D016179:Granulocyte Colony-Stimulating Factor