Duvernoy's gland secretion of Philodryas olfersii and Philodryas patagoniensis (Colubridae): neutralization of local and systemic effects by commercial bothropic antivenom (Bothrops genus).
Colubrids involved in human envenomation in Brazil are mainly from the genera Helicops, Oxyrhopus, Thamnodynastes and Philodryas. There is a relatively large number of clinical descriptions involving the Xenodontinae snakes, Philodryas olfersii and Philodryas patagoniensis, in human accidents. The most common manifestations of envenomation are local pain, swelling, erythema and ecchymosis and regional lymphadenopathy with normal coagulation. The aims of this study were to characterize the biochemical and biological properties of P. olfersii and P. patagoniensis venoms, and to investigate their immunological cross-reactivities by using both specific antisera and anti-Bothrops sp serum used for human serum therapy in Brazil, in neutralizing the lethal and hemorrhagic effects of these venoms. We show here that P. olfersii e P. patagoniensis venoms present proteolytic and haemorrhagic activities but are devoid of phospholipase A2 activity. Haemorrhage and lethality induced by P. olfersii and P. patagoniensis are associated with metal-dependent proteinases, since EDTA could block these toxic activities. P. olfersii and P. patagoniensis venoms were immunogenic and the antisera produced were able to recognize several bands in P. olfersii, P. patagoniensis venoms in Bothrops jararaca venom.
Rocha
Marisa M Teixeira
MM
Laboratório de Herpetologia, Instituto Butantan, Av. Prof. Vital Brazil, 1500, CEP 05508-900, São Paulo, SP, Brazil.
Paixão-Cavalcante
Danielle
D
Tambourgi
Denise V
DV
Furtado
Maria de Fátima D
Mde F
D000818:Animals; D000997:Antivenins / Q000276:immunology / Q000494:pharmacology*; D015153:Blotting, Western; D017837:Bothrops; D001938:Brazil; D017812:Colubridae / Q000378:metabolism*; D003429:Cross Reactions; D004492:Edetic Acid / Q000494:pharmacology; D005088:Exocrine Glands / Q000378:metabolism*; D006470:Hemorrhage / Q000139:chemically induced; D006801:Humans; D008670:Metals / Q000737:chemistry; D009500:Neutralization Tests; D010447:Peptide Hydrolases / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D012910:Snake Venoms / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013997:Time Factors
D000997:Antivenins; D008670:Metals; D012910:Snake Venoms; D004492:Edetic Acid; D010741:Phospholipases A; D054467:Phospholipases A2; D010447:Peptide Hydrolases