Effect of extremely low frequency electromagnetic fields (ELF-EMF) on Kaposi's sarcoma-associated herpes virus in BCBL-1 cells.
Association between extremely low frequency electromagnetic fields (ELF-EMF) and human cancers is controversial, and few studies have been conducted on their influence on oncogenic viruses. We studied the effects of 1 mT, 50 Hz sine waves, applied for 24-72 h, on Kaposi's sarcoma (KS)-associated herpesvirus (KSHV or HHV-8) in BCBL-1, a latently infected primary effusion lymphoma (PEL) cell line. ELF-EMF exposure did not affect the growth and viability of BCBL-1 cells, either stimulated or not with TPA. The total amount of KSHV DNA detected in ELF-EMF exposed cultures not stimulated with TPA did not differ from that of the unexposed controls (P = ns). However, in the presence of TPA stimulation, total KSHV DNA content was found higher in ELF-EMF exposed than in control BCBL-1 cultures (P = .024) at 72 h exposure, but not earlier. Viral DNA increase significantly correlated with increased mean fluorescence intensity/cell for the lytic antigen gp K8.1A/B (P < .01), but not with percentage of gp K8.1A/B-positive cells or of cells containing virions. Viral progeny produced under ELF-EMF exposure consisted mainly of defective viral particles.
Pica
Francesca
F
Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Rome, Italy. pica@uniroma2.it
Serafino
Annalucia
A
Divizia
Maurizio
M
Donia
Domenica
D
Fraschetti
Marzia
M
Sinibaldi-Salimei
Paola
P
Giganti
Maria Gabriella
MG
Volpi
Antonio
A
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects*; D004279:DNA, Viral / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004560:Electricity; D004574:Electromagnetic Fields; D019288:Herpesvirus 8, Human / Q000528:radiation effects*; D006801:Humans; D008223:Lymphoma / Q000473:pathology* / Q000821:virology*; D011829:Radiation Dosage; D014779:Virus Replication / Q000528:radiation effects*
D004279:DNA, Viral