title: Expression of tissue inhibitor of metalloproteinase during early stages of bone graft healing.
abstract: The aim of this study was to investigate the temporal expression of TIMP-1 within endochondral and intramembranous bone grafts during the early stages of healing in thirty six adult New Zealand white rabbits. Total RNA was isolated from bone grafts extracted on days 0-11 and day 14 post-grafting, for RT-PCR analysis. In situ hybridization was carried out on days 1-9 and day 14. Results showed TIMP-1 expression coincides with osteogenesis, which indicates a role for TIMP in preserving the newly formed bone during the initial stages of graft healing. Bone grafts play an important role in influencing the healing process mediated by the host tissues. The temporal expression of TIMP-1 differs in endochondral and intramembranous bone grafts. The earlier expression of TIMP-1 by endochondral bone grafts, could be the reason for the delayed vascularization while the expression of TIMP-1 by the intramembranous bone grafts, at a later stage could allow for earlier vascularization.
authors.affiliation: University of Hong Kong, Prince Philip Dental Hospital, Sai Ying Pun, Hong Kong.
authors.forename: Anne
authors.initials: A
authors.lastname: Twitty
authors.forename: A Bakr M
authors.initials: AB
authors.lastname: Rabie
authors.forename: Daisy K Y
authors.initials: DK
authors.lastname: Shum
authors.forename: Ricky W K
authors.initials: RW
authors.lastname: Wong
authors.forename: Lim K
authors.initials: LK
authors.lastname: Cheung
mesh_terms: D000818:Animals; D001857:Bone Matrix; D016025:Bone Transplantation; D001842:Bone and Bones / Q000378:metabolism; D015870:Gene Expression; D017403:In Situ Hybridization; D020782:Matrix Metalloproteinases / Q000378:metabolism; D010012:Osteogenesis; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D019714:Tissue Inhibitor of Metalloproteinases / Q000096:biosynthesis* / Q000378:metabolism / Q000502:physiology*; D019737:Transplants; D014945:Wound Healing
chemical_list: D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D019714:Tissue Inhibitor of Metalloproteinases; D012313:RNA; D020782:Matrix Metalloproteinases