Microarray analysis identifies genes preferentially expressed in the lung schistosomulum of Schistosoma mansoni.
The lung schistosomulum of Schistosoma mansoni is a validated target of protective immunity elicited in vaccinated mice. To identify genes expressed at this stage we constructed a microarray, representing 3088 contigs and singlets, with cDNA derived from in vitro cultured larvae and used it to screen RNA from seven life-cycle stages. Clustering of genes by expression profile across the life cycle revealed a number of membrane, membrane-associated and secreted proteins up-regulated at the lung stage, that may represent potential immune targets. Two promising secreted molecules have homology to antigens with vaccine and/or immunomodulatory potential in other helminths.
Dillon
Gary P
GP
Department of Biology, University of York, PO Box 373, York YO10 5YW, UK.
Feltwell
Theresa
T
Skelton
Jason P
JP
Ashton
Peter D
PD
Coulson
Patricia S
PS
Quail
Michael A
MA
Nikolaidou-Katsaridou
Nefeli
N
Wilson
R Alan
RA
Ivens
Alasdair C
AC
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology; D004270:DNA, Circular / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D017238:Genes, Helminth / Q000235:genetics*; D015801:Helminth Proteins / Q000235:genetics; D007814:Larva / Q000235:genetics; D008018:Life Cycle Stages / Q000235:genetics; D008168:Lung / Q000276:immunology*; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018510:RNA, Helminth / Q000235:genetics; D012550:Schistosoma mansoni / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
D000947:Antigens, Helminth; D004270:DNA, Circular; D018509:DNA, Helminth; D015801:Helminth Proteins; D008565:Membrane Proteins; D018510:RNA, Helminth