title: [Establishing a parabiosis animal model of two-way paradigm].
abstract: OBJECTIVE
  To establish a parabiosis model between allogenic conspecific adult mice to study two-way paradigm.
  
  
  METHODS
  Fifty-four female Balb/c mice and 54 male C57BL/6 mice were paired and equally divided into 3 groups, namely group 1 with normal saline (NS) injection, group 2 with injections of spleen cells and cyclophosphamide (CP), and group 3 injected with spleen cells, CP, and cyclosporin A (CsA). The treatments were performed by injecting the spleen cells from one of the mice in a pair into the other via tail vein and vise versa, and two days after the operation, CP (150 mg/kg) was injected intraperitoneally. Intraperitoneal CsA (30 mg/kg daily) injection was given starting from 2 days before till 7 days after the operation. Twelve of the 18 pairs of parabiosis mice in each group were separated after 1 week, and part of the skin were transplanted to each other. The maintenance of parabiosis was observed in the other 6 pairs of parabiosis mice were observed. Mixed lymphocyte reaction (MLR) and delayed-type hypersensitivity (DTH) were observed and studied in the separated mice.
  
  
  RESULTS
  The duration of parabiosis maintenance and skin survival of the group 3 was significantly longer than those in the other two groups, and group 3 showed suppressed MLR and DTH.
  
  
  CONCLUSIONS
  With the application of immunosuppressants, we have successfully established the two-way paradigm model in mice.
authors.affiliation: Department of Burn Surgery, People's Hospital of Guangdong Province, Guangzhou 510080, China. xiongbing1@hotmail.com
authors.forename: Bing
authors.initials: B
authors.lastname: Xiong
authors.forename: Hua-de
authors.initials: HD
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Lai
authors.forename: Shi-qiu
authors.initials: SQ
authors.lastname: Xiong
authors.forename: Shao-yi
authors.initials: SY
authors.lastname: Zhen
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Hui-ning
authors.initials: HN
authors.lastname: Bian
authors.forename: Zu-an
authors.initials: ZA
authors.lastname: Liu
mesh_terms: D000818:Animals; D005260:Female; D006087:Graft vs Host Reaction; D006789:Host vs Graft Reaction; D007108:Immune Tolerance / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D018448:Models, Immunological; D010227:Parabiosis