Isolation and Complementation Studies of Auxotrophic Mutants of the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium.
A variety of auxotrophic strains of Phanerochaete chrysosporium were isolated after treatment of conidia with UV and X rays. Complementation studies with these strains demonstrated heterokaryotic mycelia and conidia in this organism. Nuclear staining also showed that conidia can be mono-, di-, or multinucleate. Complementation tests allowed the separation of each auxotrophic class with the same phenotype into complementation groups.
Gold
M H
MH
Department of Chemistry and Biochemical Sciences, The Oregon Graduate Center, Beaverton, Oregon 97006.
Cheng
T M
TM
Mayfield
M B
MB