title: A multifunctional Pasteurella multocida sialyltransferase: a powerful tool for the synthesis of sialoside libraries.
abstract: A multifunctional sialyltransferase has been cloned from Pasteurella multocida strain P-1059 and expressed in E. coli as a truncated C-terminal His6-tagged recombinant protein (tPm0188Ph). Biochemical studies indicate that the obtained protein is (1) an alpha2,3-sialyltransferase (main function), (2) an alpha2,6-sialyltransferase, (3) an alpha2,3-sialidase, and (4) an alpha2,3-trans-sialidase. The recombinant tPm0188Ph is a powerful tool in the synthesis of structurally diverse sialoside libraries due to its relaxed substrate specificity, high solubility, high expression level, and multifunctionality.
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Harshal
authors.initials: H
authors.lastname: Chokhawala
authors.forename: Rebekah
authors.initials: R
authors.lastname: Karpel
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.forename: Bingyuan
authors.initials: B
authors.lastname: Wu
authors.forename: Jianbo
authors.initials: J
authors.lastname: Zhang
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Jia
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006027:Glycosides / Q000096:biosynthesis*; D006595:Hexosamines / Q000378:metabolism; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000378:metabolism; D016979:Pasteurella multocida / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism*
chemical_list: D006027:Glycosides; D006595:Hexosamines; D009438:Neuraminic Acids; D011994:Recombinant Proteins; C008322:mannosamine; D012799:Sialyltransferases