Isolation of Plasmid DNA from Butyrivibrio fibrisolvens.
A procedure based on successive precipitation of cell lysates with sodium dodecyl sulfate-NaCl and polyethylene glycol 6000 was developed which allows the isolation of plasmid DNA from Butyrivibrio fibrisolvens. A survey of B. fibrisolvens strains isolated from the bovine rumen showed that plasmids are a common feature of this species.
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Animal Research Centre, Research Branch, Agriculture Canada, Ottawa, Ontario, Canada K1A 0C6.