Detection of papillomavirus proteins and DNA in paraffin-embedded tissue sections.
The key events during the papillomavirus life cycle can be mapped in infected tissue samples by antibody detection and in situ hybridization. The ease of immuno-detection varies for different proteins and is dependent on antigen availability. Epitope exposure is sometimes necessary, because the antigen may become masked after formalin fixation and paraffin embedding of the infected tissue. Visualization of both nucleic acid and protein targets can be done simultaneously by combining in situ hybridization and immuno-detection methods.
Peh
Woei Ling
WL
Division of Virology, The National Institute for Medical Research, Mill Hill, London, UK.
Doorbar
John
J
D000956:Antigens, Viral / Q000032:analysis; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D017404:In Situ Hybridization, Fluorescence; D007202:Indicators and Reagents; D008856:Microscopy, Fluorescence / Q000379:methods; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification*
D000956:Antigens, Viral; D015342:DNA Probes; D004279:DNA, Viral; D007202:Indicators and Reagents