title: Isolation and Characterization of a Novel Atrazine Metabolite Produced by the Fungus Pleurotus pulmonarius, 2-Chloro-4-Ethylamino-6-(1-Hydroxyisopropyl)Amino-1,3,5-Triazine.
abstract: The white rot fungus Pleurotus pulmonarius exhibited metabolism of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) in liquid culture, producing the dealkylated metabolites desethylatrazine, desisopropylatrazine, and desethyl-desisopropylatrazine. A fourth, unknown metabolite was also produced. It was isolated and was identified as 2-chloro-4-ethylamino-6-(1-hydroxyisopropyl)amino-1,3,5-triazine by gas chromatography-mass spectrometry, Fourier transformed infrared spectroscopy, and H nuclear magnetic resonance analysis. The structure of this metabolite was confirmed by chemical synthesis of the compound and comparison with the fungally produced metabolite.
authors.affiliation: Migal Technological Center, Kiryat Shmona 10200, Institute of Soil and Water, Volcani Center, Bet Dagan, and Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot 76100, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Masaphy
authors.forename: D
authors.initials: D
authors.lastname: Levanon
authors.forename: J
authors.initials: J
authors.lastname: Vaya
authors.forename: Y
authors.initials: Y
authors.lastname: Henis