title: Production of Major Extracellular Enzymes during Lignocellulose Degradation by Two Streptomycetes in Agitated Submerged Culture.
abstract: Streptomyces viridosporus T7A and S. badius 252 were grown in 1 to 2% (wt/vol) slurry cultures with mineral salts solution containing 0.6% yeast extract and 100/200 mesh ground and extracted corn lignocellulose at 37 degrees C. Enzyme activities rapidly increased in the first 3 to 4 days and then declined and remained at a relatively constant level. Concentrations of endoglucanase and xylanase produced by S. badius were lower than those produced by S. viridosporus. However, the lignin-peroxidase peak concentration was threefold higher than with S. viridosporus and was obtained at 9 to 10 days of incubation. By polyacrylamide gel analysis, it was determined that peroxidases from both species consisted of four enzymes, with only one, the lignin peroxidase, having high activity. A culture pH of 8.5 was preferable for lignocellulose degradation by S. badius.
authors.affiliation: Departments of Chemical Engineering and of Bacteriology and Biochemistry, University of Idaho, Moscow, Idaho 83843.
authors.forename: T P
authors.initials: TP
authors.lastname: Adhi
authors.forename: R A
authors.initials: RA
authors.lastname: Korus
authors.forename: D L
authors.initials: DL
authors.lastname: Crawford