title: Freeze-Thawing of Aquaspirillum magnetotacticum Cells Selectively Releases Periplasmic Proteins.
abstract: Cells of the gram-negative bacterium Aquaspirillum magnetotacticum, when suspended in buffer and freeze-thawed, produced pinkish orange supernatant fluid. The fluid contained </=2.0% of total extractable outer membrane component 2-keto-3-deoxyoctonate or of the cytoplasmic membrane marker succinic dehydrogenase. Electrophoretic banding patterns and difference spectra of proteins and hemoproteins released by freeze-thawing cells were distinct from those of membrane-associated substances and similar to those of periplasmic substances obtained by applying conventional fractionation methods to this organism.
authors.affiliation: Department of Microbiology, University of New Hampshire, Durham, New Hampshire 03824.
authors.forename: L C
authors.initials: LC
authors.lastname: Paoletti
authors.forename: K A
authors.initials: KA
authors.lastname: Short
authors.forename: N
authors.initials: N
authors.lastname: Blakemore
authors.forename: R P
authors.initials: RP
authors.lastname: Blakemore