SCID-repopulating activity of human umbilical cord blood-derived hematopoietic stem and/or progenitor cells in a nonobese diabetic/Shi-SCID mice serial xenotransplantation model and immune cell activities in vitro: a comparative study of the filter method and the hydroxyethyl starch method.
BACKGROUND
A novel filter system was developed for umbilical cord blood (UCB) volume reduction. The aim of this study was to compare the functions of cryopreserved UCB cells processed by the filter and by the hydroxyethyl starch (HES) sedimentation method from the aspect of the graft quality.


METHODS
UCB specimens were divided into two portions, processed in parallel by the filter or HES, and then cryopreserved in the clinical setting. The thawed UCB specimens containing 1 x 10(5) CD34+ cells were injected into nonobese diabetic/Shi-SCID mice, and the engraftment capacity in primary and secondary transplants was assessed. The functions of natural killer (NK) cells and monocyte-derived dendritic cells (DCs) were also assayed in vitro.


RESULTS
The percentage of recovery of CD34+ cells by both methods was equivalent. In the marrow of the primary transplant recipients, the percentage of hCD45+ cells in the filter group and HES group was 58.2 +/- 31.6 and 46.5 +/- 28.4 percent, respectively (p = 0.016). The engraftment capacity and multilineage differentiation in the secondary transplantations were equal in both groups. The cytotoxic activity of the NK cells and phagocytosis activity of the DCs from both the groups were similar.


CONCLUSIONS
The filter yielded a desirable percentage of recovery of hematopoietic cells with engraftment ability in the clinical setting. Thus, it is considered that the filter system may be useful for UCB banking for cord blood transplantation.
Yasutake
Mikitomo
M
Division of Cell Processing, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Zheng
Yizhou
Y
Inoue-Nagamura
Tokiko
T
Akagawa
Eiji
E
Tokushima
Yasuo
Y
Terashima
Shuji
S
Takahashi
Tsuneo A
TA
D000818:Animals; D001793:Blood Preservation / Q000379:methods*; D036101:Cord Blood Stem Cell Transplantation; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005374:Filtration / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010587:Phagocytosis / Q000276:immunology; D016511:Severe Combined Immunodeficiency / Q000628:therapy*; D014183:Transplantation, Heterologous
D006895:Hydroxyethyl Starch Derivatives