Generation of DNA-free Escherichia coli cells by 2-aminopurine requires mismatch repair and nonmethylated DNA.
Undirected mismatch repair initiated by the incorporation of the base analog 2-aminopurine kills DNA-methylation-deficient Escherichia coli dam cells by DNA double-strand breakage. Subsequently, the chromosomal DNA is totally degraded, resulting in DNA-free cells.
Matic
Ivan
I
INSERM U571, Faculté de Médecine, Université Paris V, 156 rue Vaugirard, 75730 Paris Cedex 15, France. matic@necker.fr
Ekiert
Damian
D
Radman
Miroslav
M
Kohiyama
Masamichi
M
D015075:2-Aminopurine / Q000494:pharmacology*; D000963:Antimetabolites / Q000494:pharmacology*; D020137:Base Pair Mismatch; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D029968:Escherichia coli Proteins; D005434:Flow Cytometry; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
D000963:Antimetabolites; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D015075:2-Aminopurine; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C499294:dam protein, E coli