title: Performance limits of monolithic and packed capillary columns in high-performance liquid chromatography and capillary electrochromatography.
abstract: A method is proposed for the comprehensive characterization and comparison of columns in the high-performance liquid chromatographic (HPLC) and capillary electrochromatographic (CEC) modes. Using this approach, column parameters such as the number of plates, the eddy-diffusion and mass-transfer contributions to peak broadening, the permeability, and the analysis time are incorporated in a single graph and a comparison in terms of efficiency and speed is obtained. The chromatographic performance of silica-based and polymer-based monolithic capillary columns is discussed and a comparison is made with the performance of packed columns. Also, the potential of ultra-high-pressure liquid chromatography is discussed in this context. In the HPLC mode, the best results were obtained with silica monoliths; in the CEC mode, the low-density methacrylate-ester-based monoliths showed the best performance.
authors.affiliation: Polymer-Analysis Group, van't Hoff Institute for Molecular Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands.
authors.forename: Sebastiaan
authors.initials: S
authors.lastname: Eeltink
authors.forename: Gert
authors.initials: G
authors.lastname: Desmet
authors.forename: Gabriel
authors.initials: G
authors.lastname: Vivó-Truyols
authors.forename: Gerard P
authors.initials: GP
authors.lastname: Rozing
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Schoenmakers
authors.forename: Wim Th
authors.initials: WT
authors.lastname: Kok
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation*; D004864:Equipment and Supplies / Q000592:standards*