title: Capucin: a novel striatal marker down-regulated in rodent models of Huntington disease.
abstract: In an initial study, we compared quantitative transcriptome data across mouse brain territories using the serial analysis of gene expression method. Among the novel regional markers that we discovered, we focused on a striatum-enriched transcript with no available experimental cDNA sequence. Here, we report its cloning, gene structure, and detailed distribution in mouse brain. Quantitative RT-PCR and in situ hybridization demonstrated predominant expression in dorsolateral striatum. We therefore named it capucin for caudate-and putamen-enriched sequence. Mouse capucin is a 237-amino-acid protein, without any registered ortholog in mammalian species. It contains no recognizable motif other than two predicted carboxy-terminal transmembrane domains. When expressed in fusion with a fluorescent protein, it localized to the Golgi apparatus in two mammalian cell lines. Interestingly, we observed a significant down-regulation of capucin mRNA levels in two rodent models of Huntington disease, indicating a possible contribution to the pathogenesis of this disorder.
authors.affiliation: Laboratoire de Physiogénomique, Service de Biochimie et de Génétique Moléculaire, Commissariat à l'Energie Atomique, Saclay, 91191 Gif-sur-Yvette Cedex, France. chaldee@dsvidf.cea.fr
authors.forename: M
authors.initials: M
authors.lastname: de Chaldée
authors.forename: C
authors.initials: C
authors.lastname: Brochier
authors.forename: A
authors.initials: A
authors.lastname: Van de Vel
authors.forename: N
authors.initials: N
authors.lastname: Caudy
authors.forename: R
authors.initials: R
authors.lastname: Luthi-Carter
authors.forename: M C
authors.initials: MC
authors.lastname: Gaillard
authors.forename: J M
authors.initials: JM
authors.lastname: Elalouf
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers; D003001:Cloning, Molecular; D003342:Corpus Striatum / Q000378:metabolism*; D018076:DNA, Complementary; D004195:Disease Models, Animal; D015536:Down-Regulation; D006801:Humans; D006816:Huntington Disease / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D015415:Biomarkers; D018076:DNA, Complementary; D008565:Membrane Proteins; C511357:Syndig1l protein, mouse