[Effect of chronic enhanced external counterpulsation on arterial endothelial cells of porcine with hypercholesteremia].
OBJECTIVE
To verify whether chronic enhanced external counterpulsation (EECP) may repair artery endothelial cells (ECs) damage resulted from hypercholesteremia in pigs.


METHODS
EECP was performed for 36 hours in pigs with hypercholesteremia and the left descending artery (LDA) was isolated for scanning electron microscopy (SEM). The ECs were collected from the thoracic aorta and analyzed by proteomic technique.


RESULTS
The ECs of hypercholesteremia pigs showed irregular arrangement with obvious desquamation of coronary ECs and formation of atherosclerotic plaque, and these lesions were milder in EECP-treated pigs. Six over-expressed proteins were detected in the endothelial cells in EECP-treated pigs in comparison with those of the hypercholesteremia pigs.


CONCLUSIONS
Chronic EECP helps restore cell morphology and repair functional damage of ECs resulted from hypercholesteremia by regulating endothelial protein expressions, and consequently improves cell adhesion and intracellular metabolism and reduces EC apoptosis.
Chen
Xiao-lin
XL
Department of Cardiology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510089, China. cxlmce68@yahoo.com.cn
He
Xiao-hong
XH
Zhang
Yan
Y
Qian
Yue-tao
YT
Liang
Lu-guang
LG
Fang
Dian-qiu
DQ
Zhan
Cheng-yang
CY
Zheng
Zhen-sheng
ZS
Ma
Hong
H
D000818:Animals; D000831:Animals, Newborn; D001013:Aorta, Thoracic / Q000473:pathology; D003331:Coronary Vessels / Q000473:pathology*; D015908:Counterpulsation; D004730:Endothelium, Vascular / Q000473:pathology*; D006937:Hypercholesterolemia / Q000473:pathology*; D013552:Swine