title: Extending the array of crosslinkers suitable for the preparation of polymethacrylate-based monoliths.
abstract: The in situ preparation of monolithic capillary columns comprising copolymers of butyl methacrylate with ethylene dimethacrylate, diethylene glycol dimethacrylate, triethylene glycol dimethacrylate, and pentaerythritol tetraacrylate using thermal polymerization within 250 microm ID capillaries and their application for micro-HPLC separations of proteins has been studied. For all crosslinkers, optimization of the porogenic mixture consisting of 1-propanol and 1,4-butanediol yielded monoliths with pore sizes above 1 microm suitable for rapid separations at low back pressure. Very good separations were achieved for a protein mixture consisting of ribonuclease A, cytochrome c, myoglobin, and ovalbumin with all tested columns.
authors.affiliation: Department of Chemistry, University of California, Berkeley 94720-1460, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Nordborg
authors.forename: Frantisek
authors.initials: F
authors.lastname: Svec
authors.forename: Jean M J
authors.initials: JM
authors.lastname: Fréchet
authors.forename: Knut
authors.initials: K
authors.lastname: Irgum
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D003432:Cross-Linking Reagents / Q000737:chemistry*; D015394:Molecular Structure; D011109:Polymethacrylic Acids / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid