title: Long-term functionality of cryopreserved parathyroid autografts: a 13-year prospective analysis.
abstract: BACKGROUND
  The functional results of cryopreserved heterotopic parathyroid autotransplantation (CHPA) are not well defined. The authors evaluated the outcomes of delayed CHPA for the treatment of surgically induced hypoparathyroidism.
  
  
  METHODS
  Since November 1991, 448 parathyroid samples from 436 patients were cryopreserved at our institution. Of these, 29 patients underwent 34 CHPA procedures, with placement of 20 to 25 pieces of parathyroid tissue (approximately 50 to 75 mg) into the forearm. Outcomes were determined based on peripheral parathyroid hormone (PTH) levels and, where available, PTH gradients between grafted and nongrafted arms. Graft function results were defined as completely functional (patients with normal PTH and calcium levels off all calcium/vitamin D supplementation), partially functional (normal PTH levels and mild hypocalcemia on calcium supplementation), or nonfunctional (low PTH levels and dependent on calcium/vitamin D supplementation).
  
  
  RESULTS
  Of the 29 patients with CHPA, prospective data were available for 26 patients undergoing 30 CHPA procedures (9 patients with MEN 1, 4 with MEN 2A, 1 with MEN 2B, and 12 with sporadic hyperparathyroidism). The mean follow-up interval was 2 years. Twelve of 26 patients (46%) had completely functional grafts, 6 patients (23%) had partially functional grafts, and the remaining 8 patients (31%) had nonfunctional grafts. No patient with CHPA had graft-dependent recurrent hyperparathyroidism. Of the 14 patients (15 autografts) with MEN, 7 patients (50%) had fully functional grafts, and 2 patients (14%) had partially functional grafts. The mean cryopreservation period was 7.9 months (range, 1 week to 22 months) for functional autografts and 15.3 months (range, 2 weeks to 106 months) for nonfunctional autografts (P < .01).
  
  
  CONCLUSIONS
  Based on these data and those in previous studies, approximately 60% of delayed, cryopreserved parathyroid autografts are functional. In this study 40% autografts (46% of patients) achieved full competency off supplements. Some patients have evidence of graft function with normal PTH levels but are not normocalcemic. Results were similar for patients with MEN and nonhereditary hyperparathyroidism. The duration of cryopreservation was a significant indicator of graft failure, and no functional autograft was observed beyond 22 months of preservation. CHPA is a useful treatment modality for patients with postoperative hypocalcemia after thyroid or parathyroid surgery, who do not respond to immediate parathyroid autotransplantation.
authors.affiliation: Department of Surgery, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Cohen
authors.forename: William G
authors.initials: WG
authors.lastname: Dilley
authors.forename: Samuel A
authors.initials: SA
authors.lastname: Wells
authors.forename: Jeffrey F
authors.initials: JF
authors.lastname: Moley
authors.forename: Gerard M
authors.initials: GM
authors.lastname: Doherty
authors.forename: Gregorio A
authors.initials: GA
authors.lastname: Sicard
authors.forename: Michael A
authors.initials: MA
authors.lastname: Skinner
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Norton
authors.forename: Mary K
authors.initials: MK
authors.lastname: DeBenedetti
authors.forename: Terry C
authors.initials: TC
authors.lastname: Lairmore
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007011:Hypoparathyroidism / Q000097:blood / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal; D010280:Parathyroid Glands / Q000503:physiopathology* / Q000637:transplantation*; D010281:Parathyroid Hormone / Q000097:blood; D011446:Prospective Studies; D020127:Recovery of Function / Q000502:physiology*; D013997:Time Factors; D016042:Transplantation, Heterotopic; D016896:Treatment Outcome
chemical_list: D010281:Parathyroid Hormone