title: Production and Regeneration of Thiobacillus ferrooxidans Spheroplasts.
abstract: We investigated the production and regeneration of Thiobacillus ferrooxidans spheroplasts. Regeneration frequencies of up to 90% reversion were obtained. Attempts to transform T. ferrooxidans spheroplasts with a recombinant T. ferrooxidans plasmid were unsuccessful.
authors.affiliation: Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa.
authors.forename: M E
authors.initials: ME
authors.lastname: Barros
authors.forename: D E
authors.initials: DE
authors.lastname: Rawlings
authors.forename: D R
authors.initials: DR
authors.lastname: Woods