Investigation of early protein changes in the urinary bladder following partial bladder outlet obstruction by proteomic approach.
We investigated the pathophysiological mechanism by proteomic approach as a possible tool to detect the marker proteins to develop lower urinary tract symptoms following bladder outlet obstruction (BOO). Rats were randomized into 3 groups; control, sham operation and BOO groups. BOO group was divided into 1, 3, and 5 day-group. Conventional proteomics was performed with high resolution 2-D gel electrophoresis followed by computational image analysis and protein identification using mass spectrometry using rat urinary bladders. A comparison of bladder of BOO group with control bladder showed that three proteins of optineurin, thioredoxin and preprohaptoglobin were over-expressed in the bladder of BOO group. In addition, four proteins, such as peroxiredoxin 2, transgelin, hippocampal cholinergic neurostimulating peptide (HCNP) and beta-galactoside-binding lectin, were under-expressed in the bladder of BOO group. These data supported that downregulation of HCNP might make detrusor muscle be supersensitive to acetylcholine, up-regulation of optineurin means the protection of nerve injury, and down-regulation of transgelin means the decreased contractility of detrusor muscle. Beside these proteins, other proteins are related to oxidative stress or have a nonspecific function in this study. However more information is needed in human bladder tissue for clinical usage.
Kim
Hyung-Jee
HJ
The Proteomics Research Group, Department of Urology, Dankook University College of Medicine, Cheonan, Korea. killtumor@dankook.ac.kr
Sohng
Inho
I
Kim
Dong-Hee
DH
Lee
Dong-Cho
DC
Hwang
Cheol-Ho
CH
Park
Ji-Young
JY
Ryu
Jin-Woo
JW
D000818:Animals; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015870:Gene Expression; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation; D001743:Urinary Bladder / Q000378:metabolism*; D001748:Urinary Bladder Neck Obstruction / Q000235:genetics / Q000378:metabolism*
D011506:Proteins