title: Induction and Regeneration of Autoplasts from Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200.
abstract: Autolysis was induced to form stable, cell wall-free cells of Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200, using a complex medium containing glycine (0.4% wt/vol) and/or sucrose or glycerol (10% wt/vol) at an optimum temperature of 64 degrees C. Autoplasts of both bacteria were grown as L-phase colonies on solid medium; more than 50% of these colonies regenerated to the walled form during prolonged incubation. The removal of the cell wall was confirmed by electron microscopy.
authors.affiliation: Center for Biological Resource Recovery and Departments of Microbiology and Biochemistry, University of Georgia, Athens, Georgia 30602.
authors.forename: R
authors.initials: R
authors.lastname: Peteranderl
authors.forename: F
authors.initials: F
authors.lastname: Canganella
authors.forename: A
authors.initials: A
authors.lastname: Holzenburg
authors.forename: J
authors.initials: J
authors.lastname: Wiegel