Incorporation of Specific Fatty Acid Precursors During Spore Germination and Outgrowth in Bacillus thuringiensis.
The selective incorporation of precursors specific for individual fatty acids in germinating and outgrowing spores of Bacillus thuringiensis is described. The specific precursors utilized were [C]butyrate, -isobutyrate, -valerate, and -isovalerate, which were incorporated into even-numbered normal-chain isomers, even-numbered iso-isomers, odd-numbered normal-chain acids, and odd-numbered isohomologs, respectively. This preferential incorporation by B. thuringiensis allows the terminal carbons of specific normal and branched-chain fatty acids, contained within the cytoplasmic membrane, to be labeled with C and, potentially, C.
Nickerson
K W
KW
School of Life Sciences, University of Nebraska, Lincoln, Nebraska 68588.
Bulla
L A
LA