Determination of theophylline concentration in serum by chemiluminescent immunoassay.
OBJECTIVE
This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum.


METHODS
To measure the concentration of theophylline (n=122) and evaluate the assay.


RESULTS
The linear range of the CLIA method was 0.51-40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 micromol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L).


CONCLUSIONS
This method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.
Zhou
Mei-xia
MX
Department of Laboratory, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China. zmx00018@126.com
Guan
Cha-ying
CY
Chen
Guang
G
Xie
Xin-you
XY
Wu
Sheng-hai
SH
D001774:Blood Chemical Analysis / Q000379:methods*; D005260:Female; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008171:Lung Diseases / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013806:Theophylline / Q000097:blood*
D013806:Theophylline