title: Hydroxyapatite scaffolds for bone tissue engineering made by 3D printing.
abstract: Nowadays, there is a significant need for synthetic bone replacement materials used in bone tissue engineering (BTE). Rapid prototyping and especially 3D printing is a suitable technique to create custom implants based on medical data sets. 3D printing allows to fabricate scaffolds based on Hydroxyapatite with complex internal structures and high resolution. To determine the in vitro behaviour of cells cultivated on the scaffolds, we designed a special test-part. MC3T3-E1 cells were seeded on the scaffolds and cultivated under static and dynamic setups. Histological evaluation was carried out to characterise the cell ingrowth. In summary, the dynamic cultivation method lead to a stronger population compared to the static cultivation method. The cells proliferated deep into the structure forming close contact to Hydroxyapatite granules.
authors.affiliation: Research Center Caesar, Ludwig-Erhard-Allee 2, 53175, Bonn, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Leukers
authors.forename: Hülya
authors.initials: H
authors.lastname: Gülkan
authors.forename: Stephan H
authors.initials: SH
authors.lastname: Irsen
authors.forename: Stefan
authors.initials: S
authors.lastname: Milz
authors.forename: Carsten
authors.initials: C
authors.lastname: Tille
authors.forename: Matthias
authors.initials: M
authors.lastname: Schieker
authors.forename: Hermann
authors.initials: H
authors.lastname: Seitz
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001842:Bone and Bones; D006882:Hydroxyapatites; D051379:Mice; D023822:Tissue Engineering
chemical_list: D006882:Hydroxyapatites