Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio.
Nattokinase is a novel fibrinolytic enzyme that is considered to be a promising agent for thrombosis therapy. In this study, reverse micelles extraction was applied to purify and concentrate nattokinase from fermentation broth. The effects of temperature and phase volume ratio used for the forward and backward extraction on the extraction process were examined. The optimal temperature for forward and backward extraction were 25 degrees C and 35 degrees C respectively. Nattokinase became more thermosensitive during reverse micelles extraction. And it could be enriched in the stripping phase eight times during backward extraction. It was found that nattokinase could be purified by AOT reverse micelles with up to 80% activity recovery and with a purification factor of 3.9.
Liu
Jun-Guo
JG
College of Bioscience and Bioengineering, Hebei University of Science and Technology, 050018, Shijiazhuang, Hebei Province, China, happyliu1997@yahoo.com.cn
Xing
Jian-Min
JM
Chang
Tian-Shi
TS
Liu
Hui-Zhou
HZ
D001407:Bacillus / Q000378:metabolism*; D005591:Chemical Fractionation / Q000379:methods*; D003102:Colloids / Q000737:chemistry* / Q000302:isolation & purification*; D008823:Micelles; D012996:Solutions; D013381:Subtilisins / Q000302:isolation & purification*; D013696:Temperature; D014462:Ultrafiltration / Q000379:methods*
D003102:Colloids; D008823:Micelles; D012996:Solutions; D013381:Subtilisins; C053771:nattokinase