Development of electrochemical biosensor based on tyrosinase immobilized in composite biopolymeric film.
An electrochemical enzyme electrode for dopa and dopamine was developed via an easy and effective immobilization method. The enzyme tyrosinase was extracted from a plant source Amorphophallus companulatus and immobilized in a novel composite of two biopolymers: agarose and guar gum. This composite matrix-containing enzyme forms a self-adhering layer on the active surface of glassy carbon electrode, making it a selective and sensitive phenol sensor. Dopa and dopamine were determined by the direct reduction of biocatalytically liberated quinone species at -0.18V versus Ag/AgCl (3M KCl). The analytical characteristics of this sensor, including linear range, lower detection limit, pH, and storage stability, are described. It has reusability up to 15 cycles and a shelf life of more than 2 months.
Tembe
Sanket
S
Department of Chemistry, University of Pune, Ganeshkhind, Pune 411 007, India.
Karve
Meena
M
Inamdar
Shaukat
S
Haram
Santosh
S
Melo
Jose
J
D'Souza
Stanislaus F
SF
D031056:Amorphophallus / Q000201:enzymology; D029064:Araceae; D015374:Biosensing Techniques / Q000295:instrumentation*; D004298:Dopamine / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D005685:Galactans; D007980:Levodopa / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D008351:Mannans; D014442:Monophenol Monooxygenase; D053149:Plant Gums; D010940:Plant Proteins / Q000378:metabolism; D012685:Sepharose; D012995:Solubility
D004800:Enzymes, Immobilized; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; D010940:Plant Proteins; D007980:Levodopa; D012685:Sepharose; C007894:guar gum; D014442:Monophenol Monooxygenase; D004298:Dopamine