Expression of yeast transcriptional activator MSN1 promotes accumulation of chromium and sulfur by enhancing sulfate transporter level in plants.
MSN1 is a putative yeast transcriptional activator involved in chromium (Cr) accumulation. Here we show that overexpression of MSN1 enhances Cr and sulfur accumulation and Cr tolerance in transgenic tobacco. In addition, we found that expression of NtST1 (Nicotiana tabacum sulfate transporter 1) was elevated in MSN1- expressing transgenic tobacco, suggesting that chromate and sulfate are taken up via the sulfate transporter in plants. Supporting this, expression of NtST1 increased levels of Cr and S in Saccharomyces cerevisiae. Our findings suggest that yeast transcriptional activators can be used for developing effective metal remediators, and for improving the nutritional status of plants.
Kim
Young Jin
YJ
Department of Molecular Biology, Sejong University, 98 Kunja-Dong, Kwangjin-Gu, Seoul, Republic of Korea.
Kim
Jong Hoon
JH
Lee
Chang Eun
CE
Mok
Young Geun
YG
Choi
Jong Soon
JS
Shin
Hyoung Sun
HS
Hwang
Seongbin
S
D001673:Biodegradation, Environmental; D002840:Chromates / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017874:Immediate-Early Proteins / Q000096:biosynthesis* / Q000235:genetics; D017136:Ion Transport / Q000235:genetics; D017744:Medical Waste Disposal; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics; D000076988:Sulfate Transporters; D013431:Sulfates / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors
D002840:Chromates; D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; C490188:MSN1 protein, S cerevisiae; D017744:Medical Waste Disposal; D026901:Membrane Transport Proteins; D010940:Plant Proteins; C085609:SLC26A4 protein, human; D029701:Saccharomyces cerevisiae Proteins; D000076988:Sulfate Transporters; D013431:Sulfates; D014157:Transcription Factors