High-dimensional and large-scale phenotyping of yeast mutants.
One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.
Ohya
Yoshikazu
Y
Departments of Integrated Biosciences and Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
Sese
Jun
J
Yukawa
Masashi
M
Sano
Fumi
F
Nakatani
Yoichiro
Y
Saito
Taro L
TL
Saka
Ayaka
A
Fukuda
Tomoyuki
T
Ishihara
Satoru
S
Oka
Satomi
S
Suzuki
Genjiro
G
Watanabe
Machika
M
Hirata
Aiko
A
Ohtani
Miwaka
M
Sawai
Hiroshi
H
Fraysse
Nicolas
N
Latgé
Jean-Paul
JP
François
Jean M
JM
Aebi
Markus
M
Tanaka
Seiji
S
Muramatsu
Sachiko
S
Araki
Hiroyuki
H
Sonoike
Kintake
K
Nogami
Satoru
S
Morishita
Shinichi
S
D000199:Actins / Q000737:chemistry; D002467:Cell Nucleus / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005821:Genetic Techniques; D016681:Genome, Fungal; D023281:Genomics; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D009154:Mutation; D016366:Open Reading Frames; D010641:Phenotype; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013997:Time Factors; D015003:Yeasts / Q000235:genetics*
D000199:Actins; D005656:Fungal Proteins