title: Immunoglobulin E binding reactivity of a recombinant allergen homologous to alpha-Tubulin from Tyrophagus putrescentiae.
abstract: Storage mites may cause allergic respiratory diseases in urban areas as well as pose an occupational hazard in rural areas. Characterization of storage mite allergens is important for the development of diagnostic and therapeutic agents against mite-associated allergic disorders. Here we report on the cloning and expression of alpha-tubulin from the storage mite (Tyrophagus putrescentiae). The deduced amino acid sequence of the alpha-tubulin from the storage mite showed as much as 97.3% identity to the alpha-tubulin sequences from other organisms. The highly conserved amino acid sequences of alpha-tubulins across different species of mites may indicate that cross-reactivity for this potential allergen exists. The frequency of immunoglobulin E reactivity of this recombinant protein is 29.3% in sera from storage mite-allergic subjects.
authors.affiliation: Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors.forename: Kyoung Yong
authors.initials: KY
authors.lastname: Jeong
authors.forename: Haeseok
authors.initials: H
authors.lastname: Lee
authors.forename: Jae Sik
authors.initials: JS
authors.lastname: Lee
authors.forename: Jongweon
authors.initials: J
authors.lastname: Lee
authors.forename: In-Yong
authors.initials: IY
authors.lastname: Lee
authors.forename: Han-Il
authors.initials: HI
authors.lastname: Ree
authors.forename: Chein-Soo
authors.initials: CS
authors.lastname: Hong
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Park
authors.forename: Tai-Soon
authors.initials: TS
authors.lastname: Yong
mesh_terms: D040041:Acaridae / Q000737:chemistry / Q000276:immunology*; D000485:Allergens / Q000737:chemistry / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D007073:Immunoglobulin E / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry; D012130:Respiratory Hypersensitivity / Q000209:etiology; D017386:Sequence Homology, Amino Acid; D014404:Tubulin / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000485:Allergens; D011994:Recombinant Proteins; D014404:Tubulin; D007073:Immunoglobulin E