title: Development of electrochemical biosensor based on tyrosinase immobilized in composite biopolymeric film.
abstract: An electrochemical enzyme electrode for dopa and dopamine was developed via an easy and effective immobilization method. The enzyme tyrosinase was extracted from a plant source Amorphophallus companulatus and immobilized in a novel composite of two biopolymers: agarose and guar gum. This composite matrix-containing enzyme forms a self-adhering layer on the active surface of glassy carbon electrode, making it a selective and sensitive phenol sensor. Dopa and dopamine were determined by the direct reduction of biocatalytically liberated quinone species at -0.18V versus Ag/AgCl (3M KCl). The analytical characteristics of this sensor, including linear range, lower detection limit, pH, and storage stability, are described. It has reusability up to 15 cycles and a shelf life of more than 2 months.
authors.affiliation: Department of Chemistry, University of Pune, Ganeshkhind, Pune 411 007, India.
authors.forename: Sanket
authors.initials: S
authors.lastname: Tembe
authors.forename: Meena
authors.initials: M
authors.lastname: Karve
authors.forename: Shaukat
authors.initials: S
authors.lastname: Inamdar
authors.forename: Santosh
authors.initials: S
authors.lastname: Haram
authors.forename: Jose
authors.initials: J
authors.lastname: Melo
authors.forename: Stanislaus F
authors.initials: SF
authors.lastname: D'Souza
mesh_terms: D031056:Amorphophallus / Q000201:enzymology; D029064:Araceae; D015374:Biosensing Techniques / Q000295:instrumentation*; D004298:Dopamine / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D005685:Galactans; D007980:Levodopa / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D008351:Mannans; D014442:Monophenol Monooxygenase; D053149:Plant Gums; D010940:Plant Proteins / Q000378:metabolism; D012685:Sepharose; D012995:Solubility
chemical_list: D004800:Enzymes, Immobilized; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; D010940:Plant Proteins; D007980:Levodopa; D012685:Sepharose; C007894:guar gum; D014442:Monophenol Monooxygenase; D004298:Dopamine