Nitric oxide release during evoked neuronal activity in cerebellum slices: detection with platinized carbon-fiber microelectrodes.
Nitric oxide is an important biological messenger that particularly induces the relaxation of smooth muscle cells surrounding vessels, and, hence, controls the flow of blood. This mechanism is essential for brain function, and its fine control, termed functional hyperemia, is supposed to be realized by certain neurons that may release bursts of NO*. The aim of the present study is to examine the advantages of platinized carbon-fiber microelectrodes (5-7 microm tip diameter) for the direct and in situ electrochemical detection of NO* released by neurons into ex vivo cerebellum slices. After establishing the different analytical properties of the platinized carbon-fiber microelectrodes in vitro on NO* solutions at 50 nM to 1 mM concentration, they were characterized using DEA-NONOate solutions that chemically decompose into NO*, and therefore mimic the measurement of transient variations of NO* concentration in biological samples. This validated the present approach, so that direct, in situ ex vivo measurements of nitric oxide released by neurons in a rat cerebellar slice are presented and discussed.
Amatore
Christian
C
Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 Pasteur, 24 rue Lhomond, 75231 Paris Cedex 05, France. Christian.Amatore@ens.fr
Arbault
Stéphane
S
Bouret
Yann
Y
Cauli
Bruno
B
Guille
Manon
M
Rancillac
Armelle
A
Rossier
Jean
J
D000818:Animals; D002244:Carbon / Q000737:chemistry*; D002531:Cerebellum / Q000166:cytology* / Q000378:metabolism*; D006834:Hydrazines / Q000737:chemistry; D066298:In Vitro Techniques; D008297:Male; D008839:Microelectrodes; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism*; D010984:Platinum / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D006834:Hydrazines; D009569:Nitric Oxide; D010984:Platinum; D002244:Carbon; C084012:1,1-diethyl-2-hydroxy-2-nitrosohydrazine