Responses of MC3T3-E1 cells to three dental resin-based restorative materials.
This study aimed to examine the influences of three dental resin-based restorative materials on cells associated with hard tissue regeneration using osteoblastic MC3T3-E1 cells. A Bis-GMA-based resin composite [Clearfil AP-X (APX)], an MMA-based resin cement [Superbond C&B (SB)], and a resin-modified glass-ionomer [Fuji Ionomer Type II LC (LC)] were tested. A zinc oxide eugenol cement [Super EBA (EBA)] was included in the study for comparison. MC3T3-E1 cells were cultured on set materials for 3, 7, 14, or 21 days. Cell attachment and proliferation were observed by scanning electron microscopy, and mitochondrial dehydrogenase and alkaline phosphatase (ALP) activities of the cells were evaluated. Cell cultures on polystyrene tissue culture dishes served as controls. On APX and SB, cells demonstrated attachment, spreading, and proliferation similar to the controls. In contract, cells adhered and proliferated poorly on LC and EBA. The mitochondrial function and ALP activity of the cells were significantly suppressed (p < 0.05, Scheffe's F test) throughout the experimental period when cultured on LC or EBA, although APX and SB exhibited less inhibition. The results indicate that APX and SB are less toxic to proliferation and differentiation of MC3T3-E1, suggesting that a smaller influence on cementogenesis on these materials can be expected.
Imazato
Satoshi
S
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan. imazato@dent.osaka-u.ac.jp
Horikawa
Daisuke
D
Ogata
Korenori
K
Kinomoto
Yoshifumi
Y
Ebisu
Shigeyuki
S
D016475:3T3 Cells; D000180:Acrylic Resins; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D003470:Culture Media; D003793:Dental Restoration, Permanent / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D051379:Mice
D000180:Acrylic Resins; D003470:Culture Media; D000469:Alkaline Phosphatase