title: Single-laboratory validation of a gas chromatography-mass spectrometry method for quantitation of 15 European priority polycyclic aromatic hydrocarbons in spiked smoke flavourings.
abstract: An existing method was adapted to the purpose and validated in-house according to the IUPAC harmonised guideline for the determination of 15 EU priority polycyclic aromatic hydrocarbons (PAHs) in primary smoke condensates (PSCs) that are used to produce smoke flavourings for human consumption. Limits of detection (LOD) varied between 0.1 and 1.3 microg/kg, limits of quantitation (LOQ) between 0.5 and 4 microg/kg for the various PAHs in PSCs. The coefficient of variance of the repeatability was between 0.7% (benzo[a]pyrene) and 30% (dibenzo[a,h]pyrene) relative standard deviation, depending on the analyte. The recoveries varied between 100 and 102% (dibenzo[a,l]pyrene) and 69-83% (dibenzo[a,h]pyrene) over the analytical range of 5-35 microg/kg.
authors.affiliation: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Retieseweg 111, 2400 Geel, Belgium.
authors.forename: Rupert
authors.initials: R
authors.lastname: Simon
authors.forename: Sonja
authors.initials: S
authors.lastname: Palme
authors.forename: Elke
authors.initials: E
authors.lastname: Anklam
mesh_terms: D001551:Benz(a)Anthracenes / Q000032:analysis; D001564:Benzo(a)pyrene / Q000032:analysis; D005421:Flavoring Agents / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D015203:Reproducibility of Results; D012906:Smoke / Q000032:analysis*
chemical_list: D001551:Benz(a)Anthracenes; D005421:Flavoring Agents; D011084:Polycyclic Aromatic Hydrocarbons; D012906:Smoke; D001564:Benzo(a)pyrene; C030935:benz(a)anthracene