[Expression of Ki-67 and p53 in laryngeal squamous cell carcinomas].
OBJECTIVE
The aim of this study was to investigate the expression of p53 tumor suppressor gene and Ki-67 antigen in laryngeal squamous cell carcinoma and its relation to conventional prognostic indicators.


METHODS
p53 and Ki-67 expressions were evaluated in surgical specimens of 37 patients (34 males, 3 females; mean age 57.2 years; range 35 to 75 years) with squamous cell carcinoma of the larynx. Immunohistochemical studies were performed in routinely fixed and paraffin-embedded tissue sections using the immunoperoxidase technique and monoclonal antibodies.


RESULTS
The tumors were well-, moderately, and poorly differentiated in 5, 24, and 8 patients, respectively. Ten patients had lymph node metastasis, nine patients had vascular invasion, and 15 patients had neural invasion. Ki-67 and p53 nuclear staining was detected in 31 (83.8%) and 15 (40.5%) tumors, respectively. The incidence of Ki-67 expression was higher in patients with lymph node metastasis, but this did not exhibit significance. Neither p53 nor Ki-67 immunoexpression was in significant association with conventional clinicopathological parameters (p>0.05).


CONCLUSIONS
These data indicate that p53 and Ki-67 are not correlated with conventional prognostic parameters in squamous cell carcinoma of the larynx.
Calli
Cağlar
C
Department of Otolaryngology, Izmir Atatürk Training and Research Hospital, Izmir, Turkey. caglarcalli@hotmail.com.
Calli
Aylin
A
Pinar
Ercan
E
Oncel
Semih
S
Demirtaşoğlu
Firat
F
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53