Lipase-catalyzed synthesis of sorbitol-fatty acid esters at extremely high substrate concentrations.
Lipase-catalyzed synthesis of sorbitol-fatty acid esters was performed in eutectic media with extremely high substrate concentrations. Homogeneous eutectic melts of sorbitol and fatty acids of C6-C16 were prepared using an adjuvant mixture. Enhanced homogeneity of mixtures was confirmed by X-ray diffraction analysis. The substrate concentration was 3.63-6.67 M in the eutectic media, whereas in organic media the concentration was below 0.10 M. Esters were synthesized with an immobilized Candida antarctica lipase, and optimum conditions were analyzed. Compared to reactions in organic media, the initial reaction rate of ester synthesis and the overall productivity were significantly enhanced in eutectic media while the conversion yields were similar. Based on the kinetic analysis, highly viscous eutectic media were shown to influence the initial reaction rate and the apparent activation energy resulting in diffusion limitations.
Kim
Hyun Jung
HJ
Department of Biotechnology, College of Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, South Korea.
Youn
Sung Hun
SH
Shin
Chul Soo
CS
D002175:Candida / Q000201:enzymology*; D003198:Computer Simulation; D004789:Enzyme Activation; D004795:Enzyme Stability; D004952:Esters; D005227:Fatty Acids / Q000737:chemistry*; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D008956:Models, Chemical; D013012:Sorbitol / Q000737:chemistry*; D013379:Substrate Specificity
D004952:Esters; D005227:Fatty Acids; D013012:Sorbitol; D008049:Lipase