Effects of mushroom, Pleurotus eryngii, extracts on bone metabolism.
We performed the present study to investigate whether Pleurotus eryngii extracts (PEX) play a role in bone metabolism. PEX treatment showed increase in the alkaline phosphatase activity of the osteoblasts and in the osteocalcin mRNA expression from primary osteoblasts. PEX also increased the expression of the Runx2 gene, and the secretion of osteoprotegerin from the osteoblasts showed marked increases after treatment with PEX. In addition, PEX treatment decreased the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells and resorption areas. In vivo studies, using rats with ovariectomy-induced osteoporosis revealed that PEX alleviated the decrease in the trabecular bond mineral density.
Kim
Se-Won
SW
Oscotec Inc. 2-17, Omok-ri, Seonggeo-eup, Cheonan, Choongnam, 330-831, Republic of Korea.
Kim
Hyung-Gun
HG
Lee
Byung-Eui
BE
Hwang
Hyeon-Hwan
HH
Baek
Dong-Heon
DH
Ko
Seon-Yle
SY
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015519:Bone Density / Q000187:drug effects* / Q000502:physiology; D001854:Bone Marrow Cells / Q000378:metabolism; D001862:Bone Resorption / Q000378:metabolism; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism*; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D053244:Osteoprotegerin; D010052:Ovariectomy; D010936:Plant Extracts / Q000494:pharmacology*; D020076:Pleurotus / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism
D050659:Core Binding Factor Alpha 1 Subunit; D006023:Glycoproteins; D053244:Osteoprotegerin; D010936:Plant Extracts; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506031:Tnfrsf11b protein, mouse; C506032:Tnfrsf11b protein, rat; D000469:Alkaline Phosphatase