Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma.
A comparison study on fluoxetine (FL) and norfluoxetine (NORFL) quantitation in human plasma was carried out between the recently developed liquid chromatographic method with fluorescence detection (LC-FLD) and an earlier established liquid chromatography-mass spectrometry (LC-MS) laboratory procedure. Comparative method evaluation was based on the analysis of plasma samples obtained from Parkinsonian patients receiving 20mg of FL per day. The LC-FLD method involves a two-step liquid extraction procedure without any derivatization, followed by direct chromatography on a Zorbax C8 reversed-phase column. The analytical results are discussed in terms of the method validation and the corresponding experimental protocol (r>/=0.998; CV<9%; LOQ 20 microg/l). There was good correlation between FL, as well as NORFL, plasma levels as determined by the LC-MS and LC-FLD techniques (r=0.9597, N=16 and r=0.9852, N=14 for FL and NORFL, respectively). The results confirm that direct FL/NORFL fluorimetric determination is acceptable for routine use in pharmacokinetic and clinical studies.
Kovacevic
I
I
Department of Pharmacokinetics, Faculty of Pharmacy, University of Belgrade, Serbia and Montenegro. ivankovac9@yahoo.com
Pokrajac
M
M
Miljkovic
B
B
Jovanovic
D
D
Prostran
M
M
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005473:Fluoxetine / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods
D005473:Fluoxetine; C036139:norfluoxetine