title: Laser-induced fluorescence imaging and spectroscopy of GFP transgenic plants.
abstract: Green fluorescent protein (GFP) and other fluorescent protein bioreporters can be used to monitor transgenes in plants. GFP is a valuable marker for transgene presence and expression, but remote sensing instrumentation for stand-off detection has lagged behind fluorescent protein marker biotechnology. However, both biology and photonics are needed for the monitoring technology to be fully realized. In this paper, we describe laser-induced fluorescence imaging and laser-induced fluorescence spectroscopy of GFP-transgenic plants in ambient light towards the application of remote sensing of transgenic plants producing GFP.
authors.affiliation: Department of Plant Sciences, University of Tennessee, Knoxville, Tennesssee 37996, USA. nealstewart@utk.edu
authors.forename: C Neal
authors.initials: CN
authors.lastname: Stewart
authors.forename: Reginald J
authors.initials: RJ
authors.lastname: Millwood
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Halfhill
authors.forename: Mentewab
authors.initials: M
authors.lastname: Ayalew
authors.forename: Vinitha
authors.initials: V
authors.lastname: Cardoza
authors.forename: Mitra
authors.initials: M
authors.lastname: Kooshki
authors.forename: Gene A
authors.initials: GA
authors.lastname: Capelle
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Kyle
authors.forename: David
authors.initials: D
authors.lastname: Piaseki
authors.forename: Gregory
authors.initials: G
authors.lastname: McCrum
authors.forename: John
authors.initials: J
authors.lastname: Di Benedetto
mesh_terms: D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D007834:Lasers; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019076:Transgenes
chemical_list: D049452:Green Fluorescent Proteins