[Screening and identification of steroidal saponins-producing endophytes from Paris polyphylla var. chinensis Franch].
Two endophytic strains SS01 and SS02 with the potential for producing steroidal saponins were isolated from the underground stems of Paris polyphylla var. chinensis Franch. The TLC comparison indicated that there are 3 sports with similar R(f) between the metabolites of SS01 and the saponins of Paris polyphylla var. chinensis Franch. And there are 2 sports with similar R(f) between the metabolites of SS02 and the saponins of Paris polyphylla var. chinensis The and that biochemical SS01 Franch. characteristics of morphology, physiological belonged showed to Enterobacteriaceae and SS02 belonged to Bacillus sp.. The 16S rDNA of SS01 and SS02 were PCR and sequenced. The accessions of GenBank are AY842143 and AY842144, respectively. The two 16S rDNA phylogenetic trees were constructed by comparing with the published 16S rDNA sequences of the relative bacteria species. In the first phylogenetic tree SS01 and Cedecea davisae DSM 4568 was the closest relative with 98.9% sequence similarity, and in the second phylogenetic tree SS02 and Paenibacillus daejeonensis was the closest relative with 97.7% sequence similarity. According to the phylogenetic analysis they were identified as Cedecea davisae SS01 and Paenibacillus daejeonensis SS02, respectively.
Zhao
Ming
M
College of Life Sciences, Sichuan Normul University, Chengdu, China.
He
Sheng-rong
SR
Chen
Xiao-jing
XJ
Huang
Chun-ping
CP
Wang
Yi-ding
YD
Zhang
Xiao-jie
XJ
Feng
Ding-sheng
DS
D001406:Bacillaceae / Q000145:classification / Q000302:isolation & purification*; D001483:Base Sequence; D004365:Drugs, Chinese Herbal; D004755:Enterobacteriaceae / Q000145:classification / Q000302:isolation & purification*; D005285:Fermentation; D019668:Liliaceae / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012503:Saponins / Q000096:biosynthesis*; D013256:Steroids / Q000096:biosynthesis*
D004365:Drugs, Chinese Herbal; D012336:RNA, Ribosomal, 16S; D012503:Saponins; D013256:Steroids