Preparation of a hyaluronic acid hydrogel through polyion complex formation using cationic polylactide-based microspheres as a biodegradable cross-linking agent.
A novel hyaluronic acid (HA)-based hydrogel was prepared through polyion complex (PIC) formation between cationic polylactide (PLA)-based microspheres (MS+) and hyaluronic acid (HA-) as an anionic polyelectrolyte. The MS+ and HA formed a biodegradable PIC hydrogel (HA-/MS+) when mixed in aqueous media. The swelling behavior and mechanical properties of the PIC hydrogel could be controlled by changing the charge ratio between HA- and MS+. In addition, the HA-/MS+ PIC hydrogel resulted in a lower inflammatory response compared with a collagen hydrogel in vivo.
Arimura
Hidetoshi
H
Department of Applied Chemistry, Faculty of Engineering and High Technology Research Center, Kansai University, Suita, Osaka 564-8680, Japan.
Ouchi
Tatsuro
T
Kishida
Akio
A
Ohya
Yuichi
Y
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002412:Cations; D003094:Collagen / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000494:pharmacology; D016503:Drug Delivery Systems; D004573:Electrolytes; D006487:Hemostasis; D006820:Hyaluronic Acid / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D007249:Inflammation; D007477:Ions / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008956:Models, Chemical; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D013552:Swine; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing
D001672:Biocompatible Materials; D002412:Cations; D003432:Cross-Linking Reagents; D004573:Electrolytes; D020100:Hydrogels; D007477:Ions; D011091:Polyesters; D011108:Polymers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C033616:poly(lactide); D006820:Hyaluronic Acid; D003094:Collagen