[Capillary electrophoretic enantioseparation of arylglycine amides with highly sulfated beta-cyclodextrin as chiral selector].
Enantiomers of nine arylglycine amides synthesized were successfully separated by capillary electrophoresis (CE) using highly sulfated beta-cyclodextrin (HS-beta-CD) as a chiral selector. Baseline enantioseparation of the analytes was obtained within 6 min at neutral pH but not the commonly used acidic condition. HS-beta-CD content, buffer type and concentration, and non-chiral additive were studied and optimized for high resolution and fast speed. A reproducible running buffer system composed of 15 g/L HS-beta-CD, 0 or 10% (volume fraction) methanol and 20 mmol/L 3-(N-morpholino) propane sulfonic acid at pH 6.5 was obtained. The D-enantiomer always migrated ahead of the L-enantiomer for all the 9 pairs of arylglycine amides. The migration order was found to be dependent on the structure of the solutes.
Guo
Lei
L
Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences, Beijing 100080, China.
Lin
Shuang-Jun
SJ
Yang
Yi-Fang
YF
Qi
Li
L
Wang
Mei-Xiang
MX
Chen
Yi
Y
D000577:Amides / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000577:Amides; D047392:beta-Cyclodextrins; C031215:betadex; D005998:Glycine