title: Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri.
abstract: A coenzyme B(12)-dependent glycerol dehydratase from Lactobacillus reuteri has been purified and characterized. The dehydratase has a molecular weight of approximately 200,000, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded a single major band with a molecular weight of 52,000. K(m) values for substrates and coenzyme B(12) were in the millimolar and the submicromolar range, respectively.
authors.affiliation: Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695.
authors.forename: T L
authors.initials: TL
authors.lastname: Talarico
authors.forename: W J
authors.initials: WJ
authors.lastname: Dobrogosz