title: Enantioseparation of 2-aryloxypropionic acids on chiral porous monolithic columns by capillary electrochromatography. Evaluation of column performance and enantioselectivity.
abstract: The enantioseparation of 2-aryloxypropionic acids by capillary electrochromatography was tested on columns with a monolithic stationary phase prepared from silanized fused-silica capillaries (100 microm I.D.) by in situ copolymerization of glycidyl methacrylate, ethylene glycol dimethacrylate and methyl methacrylate in the presence of formamide and 1-propanol as the porogen solvents. The porous chiral monolithic stationary phases were prepared by reaction of the epoxy-groups at the surface of the monolith with (+)-1-(4-aminobutyl)-(5R,8S,10R)-terguride. To attain the minimum HETP values for the enantiodiscrimination of 2-phenoxypropionic acid, the influence of the composition of polymerization solution on column total porosity and efficiency was investigated. Optimum mobile phase conditions were found for all analytes tested using acetonitrile-methanol mixtures containing triethylamine and acetic acid as the buffer components. Furthermore, the chemical and mechanical stabilities of the columns were satisfactory, allowing hundreds of analyses.
authors.affiliation: Dipartimento di Chimica, Università di Roma La Sapienza, P.le Aldo Moro, 5, I-00185 Rome, Italy. antonella.messina@uniroma1.it
authors.forename: Antonella
authors.initials: A
authors.lastname: Messina
authors.forename: Miroslav
authors.initials: M
authors.lastname: Flieger
authors.forename: Fiorella
authors.initials: F
authors.lastname: Bachechi
authors.forename: Massimo
authors.initials: M
authors.lastname: Sinibaldi
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D004852:Epoxy Compounds / Q000737:chemistry; D005069:Evaluation Studies as Topic; D008689:Methacrylates / Q000737:chemistry; D011422:Propionates / Q000737:chemistry* / Q000302:isolation & purification; D011725:Pyridines / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D004852:Epoxy Compounds; D008689:Methacrylates; D011422:Propionates; D011725:Pyridines; C054176:haloxyfop; D015084:2,4-Dichlorophenoxyacetic Acid; D012822:Silicon Dioxide; C010571:dichlorprop; C007870:glycidyl methacrylate