Expression of human CMP-N-acetylneuraminic acid synthetase and CMP-sialic acid transporter in tobacco suspension-cultured cell.
Plant cells have no beta1,4-galactosylated and sialylated glycan, which plays important roles in biological functions in animal cells. Previously, we generated transgenic tobacco BY2 suspension-cultured cells that produced human beta1,4-galactosyltransferase [N.Q. Palacpac, S. Yoshida, H. Sakai, Y. Kimura, K. Fujiyama, T. Yoshida, T. Seki, Stable expression of human beta1,4-galactosyltransferase in plant cells modifies N-linked glycosylation pattern, Proc. Natl. Acad. Sci. USA 96 (1999) 4692-4697]. In this study, we introduced two critical genes encoding human CMP-N-acetylneuraminic acid synthetase and CMP-sialic acid transporter into tobacco suspension-cultured cell to pave a route for sialic biosynthetic pathway. The recombinant human proteins showed their biological activities. These results show that the plant cell can be a useful bioreactor for the production of mammalian glycoproteins.
Misaki
Ryo
R
The International Center for Biotechnology, Osaka University, Yamada-oka 2-1, Suita-shi, Osaka 565-0871, Japan.
Fujiyama
Kazuhito
K
Seki
Tatsuji
T
D002478:Cells, Cultured; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000096:biosynthesis*; D000216:N-Acylneuraminate Cytidylyltransferase / Q000235:genetics / Q000378:metabolism*; D033704:Nucleotide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
D006023:Glycoproteins; D033704:Nucleotide Transport Proteins; D011994:Recombinant Proteins; C491081:SLC35A1 protein, human; D000216:N-Acylneuraminate Cytidylyltransferase; D019158:N-Acetylneuraminic Acid