title: Effect of blood temperature on the efficacy of systemic leucodepletion during cardiopulmonary bypass: a prospective randomized clinical study.
abstract: The authors examined the effect of blood temperature within the cardiopulmonary bypass (CPB) circuit on the efficacy of an arterial line filter. Eighty patients undergoing elective, primary coronary artery bypass grafting under CPB were prospectively randomized in two equal groups. Blood temperature was kept at 35 degrees C in the first group and reduced to 28 degrees C in the second group. Twenty patients in each group had an arterial line LG6 filter attached onto CPB circuit. The other 20 patients in each group (controls) had a non-leukocyte-depleting filter. Blood samples (10 ml) were taken before CPB, at 5 minutes on CPB, at 30 minutes on CPB, 5 minutes after aortic clamp removal, and 6 hours postoperatively. Leucocytes were counted under light microscopy. Activated leucocytes were identified using nitroblue tetrazolium staining. Patients undergoing leucodepletion had significantly lower total and activated leukocyte counts than control patients in both groups (p < 0.05). Patients having a leukocyte-depleting filter at a CPB temperature of 35 degrees C had significantly lower total leukocyte counts (p < 0.05) than those having a leukocyte-depleting filter at a CPB temperature of 28 degrees C (p < 0.05). However, there were not statistically significant differences in the activated leukocyte counts between the two leucodepleted groups (p > 0.05). This study shows that warm blood temperature within the CPB circuit has a positive effect on the overall leucodepleting efficacy of the LG6 filter. Activated leucocytes, however, seem to be depleted at similar rates irrespective of the blood temperature in the CPB circuit.
authors.affiliation: Department of Cardiac Surgery, The General Hospital, Southampton, UK. alexiou486@aol.com
authors.forename: Christos
authors.initials: C
authors.lastname: Alexiou
authors.forename: Stuart
authors.initials: S
authors.lastname: Sheppard
authors.forename: David
authors.initials: D
authors.lastname: Smith
authors.forename: Roz
authors.initials: R
authors.lastname: Gibbs
authors.forename: Marcus P
authors.initials: MP
authors.lastname: Haw
mesh_terms: D000368:Aged; D001769:Blood; D001831:Body Temperature; D001026:Coronary Artery Bypass / Q000009:adverse effects / Q000379:methods; D005260:Female; D006801:Humans; D007036:Hypothermia, Induced; D007249:Inflammation / Q000517:prevention & control; D047589:Leukocyte Reduction Procedures; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies