Analysis of Dictyostelium mucoroides Macrocysts by Using a Simple Breakage and Fractionation Procedure.
A method is described for the breakage and fractionation of Dictyostelium macrocysts. It involves vortexing with large glass beads to disrupt the macrocyst wall followed by differential low-speed centrifugation. It yields fractions containing purified wall material and intact endocytes. The endocytes can themselves be disrupted by using smaller glass beads.
North
M J
MJ
Department of Biological and Molecular Sciences, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom, and Department of Biological Sciences, University of Windsor, Windsor, Ontario N9B 3P4, Canada.
Cotter
D A
DA