A cDNA-microarray analysis of camptothecin resistance in glioblastoma cell lines.
Chemotherapy, as generally available, is of a limited value in curing malignant brain tumors (gliomas), which often develop resistance to drugs, becoming completely unresponsive to any standard therapeutic approach. Camptothecins, a family of topoisomerase I inhibitor drugs, represent a new promising treatment strategy and are currently under evaluation for testing the clinical efficacy. We selected a CPT-resistant sub-line (U87CPT-R) from U87-MG grade III-IV astrocytoma cells, and compared the expression profile of the two cell lines by cDNA-microarray, as a preliminary screening of the molecular mechanisms involved in the acquisition of CPT resistance in glioma cells. The relevant role of IL-1 beta overproduction as well as a generalised up-regulation of genes implicated in angiogenesis and inflammatory response are discussed in details.
Morandi
Elena
E
Fenice Environmental Research Center, Via Ciro Menotti 48, 48023 Marina di Ravenna, Ravenna, Italy.
Zingaretti
Chiara
C
Chiozzotto
Daniela
D
Severini
Cinzia
C
Semeria
Alessandro
A
Horn
Wolfango
W
Vaccari
Monica
M
Serra
Roberto
R
Silingardi
Paola
P
Colacci
Annamaria
A
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000473:pathology*; D002166:Camptothecin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D007249:Inflammation; D009389:Neovascularization, Pathologic; D020411:Oligonucleotide Array Sequence Analysis; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D000972:Antineoplastic Agents, Phytogenic; D002166:Camptothecin