title: Application of C Nuclear Magnetic Resonance To Elucidate the Unexpected Biosynthesis of Erythritol by Leuconostoc oenos.
abstract: Natural-abundance C nuclear magnetic resonance (C-NMR) revealed the production of erythritol and glycerol by nongrowing cells of Leuconostoc oenos metabolizing glucose. The ratio of erythritol to glycerol was strongly influenced by the aeration conditions of the medium. The elucidation of the metabolic pathway responsible for erythritol production was achieved by C-NMR and H-NMR spectroscopy using specifically C-labelled d-glucose. The H-NMR spectrum of the cell supernatant resulting from the metabolism of [2-C]glucose showed that only 75% of the glucose supplied was metabolized heterofermentatively and that the remaining 25% was channelled to the production of erythritol. The synthesis of this polyol resulted from the reduction of the C-4 moiety of the intermediate fructose 6-phosphate. Oxygen has an inhibitory effect on the production of erythritol by L. oenos. Preaeration of a suspension of nongrowing cells of L. oenos resulted in 30% less erythritol and in 70% more glycerol formed during the anaerobic metabolism of glucose. The anaerobic production of erythritol from glucose was also found in growing cultures of L. oenos, although to a smaller extent.
authors.affiliation: Centro de Tecnologia Quimica e Biológica, Rua da Quinta Grande 6, Apartado 127, 2780 Oeiras, Estação Nacional de Tecnologia dos Produtos Agrários, Lisbon, Estação Vitivinicola Nacional, Instituto Nacional de Investigação Agrária, 2575 Runa, and Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2825 Monte da Caparica, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Veiga-Da-Cunha
authors.forename: P
authors.initials: P
authors.lastname: Firme
authors.forename: M V
authors.initials: MV
authors.lastname: Romão
authors.forename: H
authors.initials: H
authors.lastname: Santos