title: Preparative separation of cichoric acid from Echinacea purpurea by pH-zone-refining counter-current chromatography.
abstract: pH-zone-refining counter-current chromatography was successfully applied to the separation of cichoric acid from Echinacea Purpurea (L.) Moench. A 3.0 g quantity of sample was separated using the following two-phase solvent system: MtBE-CH3CN-water (4:1:5, v/v), 10 mM trifluoroacetic acid in organic stationary phase and 10 mM ammonia in aqueous mobile phase. The obtained fractions were analyzed by high-performance liquid chromatography and negative ion electrospray ionization mass spectrometry. Double separations were performed with the same solvent system yielding 563 mg cichoric acid at 95.6% purity.
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China. wangx@keylab.net
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Yanling
authors.initials: Y
authors.lastname: Geng
authors.forename: Fuwei
authors.initials: F
authors.lastname: Li
authors.forename: Qianshan
authors.initials: Q
authors.lastname: Gao
authors.forename: Xingang
authors.initials: X
authors.lastname: Shi
mesh_terms: D002109:Caffeic Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D020900:Echinacea / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D013386:Succinates / Q000302:isolation & purification*
chemical_list: D002109:Caffeic Acids; D013386:Succinates; C100435:chicoric acid