[Peptide synthesis in organic media with the use of subtilisin 72 immobilized on a poly(vinyl alcohol) cryogel].
Subtilisin 72 serine protease (EC 3.4.21.14) immobilized on a poly(vinyl alcohol) cryogel was used as a catalyst in the syntheses of N-protected peptide p-nitroanilides of the general formulas Z(or Boc)-Xaa-Phe-pNA (Xaa = Leu or Ala), Z-Ala-Xaa-Yaa-pNA (Xaa = Leu or Ala; Yaa = Leu or Phe), and Z-Ala-Ala-Xaa-Yaa-pNA (Xaa = Leu, Arg, or Gly; Yaa = Phe, Leu, Gly, Asp, or Glu). The syntheses were carried out in DMF-acetonitrile mixtures. A number of protected di-, tri-, and tetrapeptides were prepared in yields up to 99%. The syntheses were found to retain stereoselectivity under the conditions studied. The activation of carboxyl group of the acylating component was shown to have a positive effect upon the coupling rate. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2005, vol. 31, no. 6; see also http://www.maik.ru.
Beliaeva
A V
AV
Bacheva
A V
AV
Oksenoĭt
E S
ES
Lysogorskaia
E N
EN
Lozinskiĭ
V I
VI
Filippova
I Iu
IIu
D000596:Amino Acids / Q000737:chemistry; D001412:Bacillus subtilis / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005782:Gels; D010455:Peptides / Q000138:chemical synthesis*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D013237:Stereoisomerism; D013381:Subtilisins / Q000737:chemistry*
D000596:Amino Acids; D004800:Enzymes, Immobilized; D005782:Gels; D010455:Peptides; D011142:Polyvinyl Alcohol; D013381:Subtilisins