Initial steps in the degradation of phosphinothricin (glufosinate) by soil bacteria.
Three hundred bacterial isolates from soil were tested for resistance against phosphinothricin [PPT; dl-homoalanin-4-yl(methyl)phosphinic acid], the active ingredient of the herbicide BASTA. Eight resistant bacterial strains and Escherichia coli were analyzed for PPT-transforming activities. At least three different enzymatic reactions could be detected in cell extracts. In six strains an acetyltransferase was active, synthesizing N-acetyl-PPT in the presence of PPT and acetyl coenzyme A. All strains could degrade PPT to its corresponding 2-oxoacid {2-oxo-4-[(hydroxy)(methyl)phosphinoyl] butyric acid} by transamination. Rhodococcus sp., the only tested strain that was able to utilize PPT as a sole source of nitrogen, formed 2-oxo-4[(hydroxy)(methyl)phosphinoyl]butyric acid by oxidative deamination. This enzymatic activity was inducible by l-glutamic acid or PPT itself but not in the presence of NH(4). d-PPT transformation was not detectable in any of the investigated strains.
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HOECHST AG, 6230 Frankfurt/M. 80, and Institut für Bodenbiologie, Bundesforschungsanstalt für Landwirtschaft, 3300 Braunschweig, Federal Republic of Germany.
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