High Production of Thermostable beta-Galactosidase of Bacillus stearothermophilus in Bacillus subtilis.
By cloning the beta-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis, enzyme production was enhanced 50 times. beta-Galactosidase could be purified to 80% homogeneity by incubating the cell extract of B. subtilis at 70 degrees C for 15 min, followed by centrifugation to remove the denatured proteins. Because of its heat stability and ease of production, beta-galactosidase is suitable for application in industrial processes.
Hirata
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H
Department of Fermentation Technology, Osaka University, Yamada-oka, Suita-shi, Osaka 565, and Wakamoto Pharmaceutical Co., Ltd., Nihonbashimuromachi 1-8, Chuo-ku, Tokyo 103, Japan.
Negoro
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S
Okada
H
H