Analysis of free amino acids in microbially colonized sandstone by precolumn phenyl isothiocyanate derivatization and high-performance liquid chromatography.
A fast, sensitive method for extraction and analysis of soluble free amino acids from microbially colonized sandstone is described. After precolumn phenyl isothiocyanate (PITC) derivatization, the PITC-amino acids were identified and quantified by reversed-phase high-performance liquid chromatography. This kind of analysis could be used to elucidate the role and function of amino acids in the nutrition of epi- and endolithic microorganisms active in biological weathering processes.
Siebert
J
J
Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität, D-2300 Kiel, Federal Republic of Germany.
Palmer
R J
RJ
Hirsch
P
P