Fluorescent sensors for rapid monitoring of intracellular cGMP.
Sensors based on fluorescence resonance energy transfer (FRET) are powerful tools to monitor signaling events in living mammalian cells. Here we describe development and use of new sensors for cyclic GMP (cGMP) based on cGMP binding domains from cGMP-dependent protein kinase I (GKI) and from phosphodiesterases (PDEs). The temporal and spatial resolution attained with the new sensors is superior to that of existing techniques, and permits direct recording and imaging of rapid cGMP-signaling events.
Nikolaev
Viacheslav O
VO
Institute of Pharmacology and Toxicology, Versbacher Str. 9, D-97078 Würzburg, Germany.
Gambaryan
Stepan
S
Lohse
Martin J
MJ
D015374:Biosensing Techniques; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000032:analysis; D006152:Cyclic GMP / Q000032:analysis*; D062965:Cyclic GMP-Dependent Protein Kinase Type I; D017869:Cyclic GMP-Dependent Protein Kinases / Q000737:chemistry* / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry* / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D017384:Sequence Deletion
D000242:Cyclic AMP; D062965:Cyclic GMP-Dependent Protein Kinase Type I; D017869:Cyclic GMP-Dependent Protein Kinases; C569718:PRKG1 protein, human; D010727:Phosphoric Diester Hydrolases; D006152:Cyclic GMP