Ganglioside GM1-binding peptides as adjuvants of antigens inoculated by the intranasal route.
Forty-five GM1-binding peptides were identified using phage-displayed peptides libraries of random peptides. Most have a motif containing a hydrophobic amino acid followed by a serine (S). Based on a GM1-binding assays, two of these GM1-binding peptides (named 15 and 40) were chosen to investigate its immunostimulatory properties when chemically coupled to antigens. Mice intra-nasally (i.n.) vaccinated with some of these complexes developed a better local and systemic antibody response than mice i.n. vaccinated with the respective uncoupled antigens. The efficiency of the complex GM1-binding peptide-antigen strongly depends on the composition and structure of both of the components of the complex.
Montaner
Alejandro D
AD
Instituto de Investigaciones Biomédicas, Fundación Pablo Cassará, Saladillo 2452, Buenos Aires C1440FFX, Argentina.
De Nichilo
Analía
A
Elias
Fernanda
F
Rodríguez
Juan M
JM
Fló
Juan M
JM
López
Ricardo A
RA
Zorzopulos
Jorge
J
Frank
Ronald
R
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D000956:Antigens, Viral / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005677:G(M1) Ganglioside / Q000378:metabolism*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000008:administration & dosage / Q000276:immunology; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism; D019151:Peptide Library; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D011485:Protein Binding; D013296:Streptococcus pneumoniae / Q000276:immunology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D000942:Antigens, Bacterial; D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D019151:Peptide Library; D010455:Peptides; D005677:G(M1) Ganglioside; D009439:Neuraminidase