title: Enhanced solubilization of bovine serum albumin in reverse micelles by compressed CO2.
abstract: The effect of compressed CO2 on the solubilization of bovine serum albumin (BSA) in water/sodium bis-(2-ethylhexyl) sulfosuccinate (AOT)/isooctane reverse micelles was studied by observing phase behavior and recording UV-visible spectra under different conditions. The pH values within the water cores of reverse micelles at different CO2 pressures were also determined. The solubilization capacity of the reverse micelles for the protein increased considerably as CO2 pressure increased within the low-pressure range, but decreased at higher CO2 pressures, so that the micelles eventually lost their ability to solubilize the protein. The effect of CO2 on the stability of the reverse micelles played an important role in the relationship between pressure and protein solubility. A "multicomplex" model was proposed to explain these effects. The different solublization capacities within different pressure ranges demonstrates the unique advantage of using compressed CO2 in the extraction of proteins with reverse micelles.
authors.affiliation: The Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080 (China).
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Feng
authors.forename: Jianling
authors.initials: J
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Buxing
authors.initials: B
authors.lastname: Han
authors.forename: Dong
authors.initials: D
authors.lastname: Shen
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents; D014867:Water / Q000737:chemistry
chemical_list: D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water; D002245:Carbon Dioxide; D012710:Serum Albumin, Bovine