Use of immunogold labelling with scanning electron microscopy to identify phytopathogenic bacteria on leaf surfaces.
Scanning electron microscopy coupled with high-resolution back-scattered electron imaging was used to detect gold-labelled specific immunoglobulins attached to epiphytic bacteria. Strains of Xanthomonas citri and X. campestris pv. citrumelo were specifically identified on grapefruit leaf surfaces when labelled with homologous gold-labelled immunoglobulins.
Davis
C L
CL
Citrus Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Lake Alfred, Florida 33850.
Brlansky
R H
RH