title: HCO(3) Fixation by Naturally Occurring Tufts and Pure Cultures of Thiothrix nivea.
abstract: Naturally occurring tufts of the mixotroph Thiothrix nivea blanketed the East Everglades (Dade County, Fla.) Chekika artesian well and runoff areas. The rate of HCO(3) fixation by these Thiothrix tufts was determined to be 14.0 +/- 5.4 nmol of HCO(3) per min per mg of dry weight, which reflected a growth rate of 5.0%/h. The addition of 10 mM glucose, ribose, acetate, or pyruvate or 0.05% Casamino Acids (Difco Laboratories, Detroit, Mich.) did not appear to alter the HCO(3) fixation rate. Whereas 1 mM acetate or 10 mM lactate, ethanol, glycerol, alpha-ketoglutarate, succinate, fumarate, or citrate slightly stimulated HCO(3) fixation, 5 to 10 mM malate inhibited HCO(3) fixation by 90%. Pure Thiothrix cultures isolated from Chekika fixed HCO(3) at rates as high as 29.9 +/- 2.8 nmol of HCO(3) per min per mg of dry weight in the presence of growth medium. Malate did not have a suppressive effect but rather slightly stimulated in vivo HCO(3) fixation.
authors.affiliation: Department of Biological Sciences, Florida International University, Miami, Florida 33199.
authors.forename: M F
authors.initials: MF
authors.lastname: McGlannan
authors.forename: J C
authors.initials: JC
authors.lastname: Makemson