Detection and quantitation of HPV DNA replication by Southern blotting and real-time PCR.
This provides a brief introduction into the mechanism of DNA replication by the E1 and E2 proteins and describes the traditional Southern blotting technique that is used to monitor E1- and E2-mediated DNA replication. It also includes a novel real-time polymerase chain reaction (PCR) approach for monitoring E1- and E2-mediated DNA replication that has enhanced sensitivity and quantitation compared with Southern blotting, and a discussion of when to use the Southern blotting and real-time PCR techniques.
Morgan
Iain M
IM
Institute of Comparative Medicine, University of Glasgow, Scotland.
Taylor
Ewan R
ER
D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods; D002460:Cell Line; D004265:DNA Helicases / Q000235:genetics; D017931:DNA Primers; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D019281:Dimerization; D006801:Humans; D007700:Kinetics; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping / Q000379:methods; D014162:Transfection
D017931:DNA Primers; D004279:DNA, Viral; D004265:DNA Helicases