title: Biochemical properties of the Vibrio harveyi CgtAV GTPase.
abstract: Bacteria encode a number of relatively poorly characterized GTPases, including the essential, ribosome-associated Obg/CgtA proteins. In contrast to Ras-like proteins, it appears that the Obg/CgtA proteins bind guanine nucleotides with modest affinity and hydrolyze GTP relatively slowly. We show here that the Vibrio harveyi CgtA(V) exchanges guanine nucleotides rapidly and has a modest affinity for nucleotides, suggesting that these features are a universal property of the Obg/CgtA family. Interestingly, CgtA(V) possesses a significantly more rapid GTP hydrolysis rate than is typical of other family members, perhaps reflecting the diversity and specificity of bacterial ecological niches.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, MI 48109, USA.
authors.forename: A E
authors.initials: AE
authors.lastname: Sikora
authors.forename: K
authors.initials: K
authors.lastname: Datta
authors.forename: J R
authors.initials: JR
authors.lastname: Maddock
mesh_terms: D001665:Binding Sites; D001671:Biochemistry / Q000379:methods; D020558:GTP Phosphohydrolases / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D006150:Guanine Nucleotides / Q000737:chemistry*; D006160:Guanosine Triphosphate / Q000737:chemistry*; D006868:Hydrolysis; D011485:Protein Binding; D014733:Vibrio / Q000201:enzymology*
chemical_list: D006150:Guanine Nucleotides; D006147:Guanine; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases