Viral transgenesis of embryonic cell cultures from the freshwater microcrustacean Daphnia.
The aim of this study was to determine whether a recombinant vesicular stomatitis virus (VSV) vector encoding a transgene could be used to infect and express a foreign gene in embryonic primary cell cultures derived from the freshwater microcrustacean Daphnia, the most widely used ecotoxicological model organism. To facilitate the evaluation of gene transfer, a reproducible method for establishing primary cultures from Daphnia embryonic tissues was developed. Within 24 hr after infection, transgene expression could be detected in cell culture. VSV was found to replicate in the cells with no apparent cytopathic effect. Here we report the first evidence of gene transfer and foreign gene expression in cultures of Daphnia embryonic cells using a recombinant viral vector.
Robinson
Christy D
CD
Department Tropical Medicine, Tulane University, New Orleans, Louisiana 70112, USA.
Lourido
Sebastian
S
Whelan
Sean P
SP
Dudycha
Jeffry L
JL
Lynch
Michael
M
Isern
Sharon
S
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D002478:Cells, Cultured; D003621:Daphnia / Q000166:cytology / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000166:cytology; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D023421:Models, Animal; D011994:Recombinant Proteins; D014161:Transduction, Genetic / Q000379:methods*; D019076:Transgenes / Q000235:genetics; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics*
D011994:Recombinant Proteins; C485192:green fluorescent protein, Aequorea victoria; D049452:Green Fluorescent Proteins