title: Novel three-dimensional organoid model for evaluation of the interaction of uropathogenic Escherichia coli with terminally differentiated human urothelial cells.
abstract: Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced alpha-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.
authors.affiliation: Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Rm. B4052, 4301 Jones Bridge Rd., Bethesda, MD 20814-4799, USA.
authors.forename: Yarery C
authors.initials: YC
authors.lastname: Smith
authors.forename: Kerian K
authors.initials: KK
authors.lastname: Grande
authors.forename: Susan B
authors.initials: SB
authors.lastname: Rasmussen
authors.forename: Alison D
authors.initials: AD
authors.lastname: O'Brien
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D009940:Organoids / Q000382:microbiology / Q000473:pathology* / Q000648:ultrastructure; D019459:Urothelium / Q000382:microbiology* / Q000473:pathology* / Q000648:ultrastructure; D018474:Weightlessness Simulation