Improved fixation of cellulose-acetate reverse-osmosis membrane for scanning electron microscopy.
Fixation of cellulose-acetate membranes with either glutaraldehyde-osmium tetroxide or glutaraldehyde-ruthenium tetroxide resulted in extensive electron beam damage. Beam damage was eliminated and the bacterial surface structure was preserved, however, when cellulose-acetate membranes were fixed with glutaraldehyderuthenium tetroxide and treated successively with thiocarbohydrazide and osmium tetroxide.
Kutz
S M
SM
Department of Microbiology and Immunology and Electron Microscope Facility, College of Agriculture, University of Arizona, Tucson, Arizona 85721.
Bentley
D L
DL
Sinclair
N A
NA