title: 5,7-DHT lesion of the dorsal raphe nuclei impairs object recognition but not affective behavior and corticosterone response to stressor in the rat.
abstract: Previous studies with acute tryptophan depletion, leading to transient central 5-HT reductions, showed no effects on affective behavior but impaired object memory. In the present study, the behavioral effects of a 5,7-dihydroxytryptamine (5,7-DHT) lesion in the dorsal raphe were evaluated in animal models of anxiety (open field test), depression (forced swimming test), behavioral inhibition (discrete fixed interval test) and cognition (object recognition task). The corticosterone response to a stress condition was examined at several intervals after 5,7-DHT treatment. The substantial reduction in neuronal 5-HT markers in the dorsal raphe did not affect anxiety-related, depressive-like or impulsive behavior. Compared to the SHAM group, the lesioned rats showed a lower response latency to obtain a reward, indicating a quick and accurate reaction to a stimulus. No differences were found in the progressive ratio test for food motivation. A marked impairment in object recognition was found. The 5,7-DHT treatment did not affect the corticosterone response to a stressful situation. Overall, these results corroborate studies with acute tryptophan depletion suggesting a role of 5-HT in object memory, but not affective behavior.
authors.affiliation: Department of Psychiatry and Neuropsychology (Basal Neuroscience), University of Maastricht, Maastricht, The Netherlands. cindy.lieben@AH.unimaas.nl
authors.forename: Cindy K J
authors.initials: CK
authors.lastname: Lieben
authors.forename: Harry W M
authors.initials: HW
authors.lastname: Steinbusch
authors.forename: Arjan
authors.initials: A
authors.lastname: Blokland
mesh_terms: D015116:5,7-Dihydroxytryptamine; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D001930:Brain Injuries / Q000139:chemically induced / Q000503:physiopathology*; D003216:Conditioning, Operant / Q000187:drug effects / Q000502:physiology; D003345:Corticosterone / Q000097:blood*; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D007266:Inhibition, Psychological; D008297:Male; D011903:Raphe Nuclei / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D021641:Recognition, Psychology / Q000187:drug effects / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism; D013312:Stress, Physiological / Q000097:blood*; D013550:Swimming / Q000502:physiology
chemical_list: D015116:5,7-Dihydroxytryptamine; D012701:Serotonin; D003345:Corticosterone