Separation of muscle membrane from alkali-solubilized fish muscle proteins.
Treatment with Ca2+ and citric acid improved membrane removal from muscle homogenates solubilized at pH 10.5 by centrifugation at 4000 g for 15 min. The percentage of phospholipid removed from muscle homogenates increased with increasing Ca2+ concentrations at 1 mM citric acid. More than 85% phospholipid and 45% protein in the muscle homogenates were removed at Ca2+ concentrations of >20 mM in the presence of 1 mM citric acid. At 8 mM Ca2+, addition of citric acid at 5 mM improved phospholipid removal to approximately 78% from 58% in its absence. Because treatment with 8 mM Ca2+ alone can remove significant amounts of phospholipid, it is likely that Ca2+ played the major role in membrane removal in muscle homogenates solubilized at pH 10.5.
Liang
Yong
Y
Massachusetts Agricultural Experiment Station, Department of Food Science, University of Massachusetts/Amherst, Marine Station, Gloucester, Massachusetts 01930, USA. yliang@ufl.edu
Hultin
Herbert O
HO
D000468:Alkalies; D000818:Animals; D002118:Calcium / Q000737:chemistry; D002855:Chromatography, Thin Layer; D019343:Citric Acid / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008566:Membranes / Q000737:chemistry; D009124:Muscle Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010743:Phospholipids / Q000032:analysis
D000468:Alkalies; D009124:Muscle Proteins; D010743:Phospholipids; D019343:Citric Acid; D002118:Calcium