Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent.
Harmful algal blooms, caused by rapid growth and accumulation of certain microalgae in the ocean, pose considerable impacts on marine environments, aquatic industries and even public health. Here, we present the 7.2-megabase genome of the marine bacterium Hahella chejuensis including genes responsible for the biosynthesis of a pigment which has the lytic activity against a red-tide dinoflagellate. H.chejuensis is the first sequenced species in the Oceanospiralles clade, and sequence analysis revealed its distant relationship to the Pseudomonas group. The genome was well equipped with genes for basic metabolic capabilities and contained a large number of genes involved in regulation or transport as well as with characteristics as a marine heterotroph. Sequence analysis also revealed a multitude of genes of functional equivalence or of possible foreign origin. Functions encoded in the genomic islands include biosynthesis of exopolysacchrides, toxins, polyketides or non-ribosomal peptides, iron utilization, motility, type III protein secretion and pigmentation. Molecular structure of the algicidal pigment, which was determined through LC-ESI-MS/MS and NMR analyses, indicated that it is prodigiosin. In conclusion, our work provides new insights into mitigating algal blooms in addition to genetic make-up, physiology, biotic interactions and biological roles in the community of a marine bacterium.
Jeong
Haeyoung
H
Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 305-600, Republic of Korea.
Yim
Joung Han
JH
Lee
Choonghwan
C
Choi
Sang-Haeng
SH
Park
Yon Kyoung
YK
Yoon
Sung Ho
SH
Hur
Cheol-Goo
CG
Kang
Ho-Young
HY
Kim
Dockyu
D
Lee
Hyun Hee
HH
Park
Kyun Hyang
KH
Park
Seung-Hwan
SH
Park
Hong-Seog
HS
Lee
Hong Kum
HK
Oh
Tae Kwang
TK
Kim
Jihyun F
JF
D000222:Adaptation, Physiological; D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001483:Base Sequence; D004141:Dinoflagellida / Q000187:drug effects*; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D016680:Genome, Bacterial; D023281:Genomics; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010802:Phylogeny; D010839:Phytoplankton / Q000187:drug effects*; D011353:Prodigiosin / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D014774:Virulence / Q000235:genetics
D000981:Antiprotozoal Agents; D011353:Prodigiosin