Glutamate metabolizing enzymes in prefrontal cortex of Alzheimer's disease patients.
Amounts of glutamate metabolizing enzymes such as glutamate dehydrogenase (GDH), glutamine synthetase (GS), GS-like protein (GSLP), and phosphate-activated glutaminase (PAG) were compared in prefrontal cortex of control subjects and patients with Alzheimer disease (AD). The target proteins were quantified by ECL-Western immunoblotting in extracts from brain tissue prepared by two different techniques separating enzymes preferentially associated with cytoplasm (GDH I and II isoenzymes, GS, and partially GSLP) and membrane (GDH III, PAG, and partially GSLP) fractions. Amounts of all listed enzymes were found significantly increased in the patient group compared with controls. Some links between the measured values were observed in the control, but not in the AD patient group. The results may suggest for the pathological interruption of regulatory relations between distinct enzymes of glutamate metabolism in brain of AD patients.
Burbaeva
Gulnur Sh
GSh
Laboratory of Neurochemistry, Mental Health Research Center, Russian Academy of Medical Sciences, Moscow, Russia. burbaeva@yahoo.com
Boksha
Irina S
IS
Tereshkina
Elena B
EB
Savushkina
Olga K
OK
Starodubtseva
Lubov' I
LI
Turishcheva
Marina S
MS
D000368:Aged; D000544:Alzheimer Disease / Q000201:enzymology*; D019732:Amide Synthases / Q000378:metabolism; D005260:Female; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D005972:Glutaminase / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism; D017397:Prefrontal Cortex / Q000201:enzymology*; D013223:Statistics as Topic; D014020:Tissue Extracts / Q000378:metabolism
D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D014020:Tissue Extracts; D018698:Glutamic Acid; D005969:Glutamate Dehydrogenase; D005972:Glutaminase; D019732:Amide Synthases; C417025:glutamine synthetase-like protein, human; D005974:Glutamate-Ammonia Ligase