title: Gene transfer to trabecular meshwork endothelium via direct injection into the Schlemm canal and in vivo toxicity study.
abstract: OBJECTIVE
  Our aim was to investigate the efficiency of adenoviral gene transfer via direct injection into the Schlemm canal ex vivo in human donor eyes and to examine the effect of human MMP-3 transgene expression in a rat model in vivo.
  
  
  METHODS
  A viscocanalostomy-like operation was performed and adenoviral vector encoding for MMP-3 and green fluorescent protein was injected into human Schlemm canal or rat anterior chamber.
  
  
  RESULTS
  Transgene expression was high in trabecular meshwork endothelium in human donor eyes. In vivo, adenovirus caused dose-dependent inflammation.
  
  
  CONCLUSIONS
  Direct injection of adenoviral vectors into the Schlemm canal has potential in glaucoma treatment.
authors.affiliation: Zentrum für Augenheilkunde, Abteilung für Erkrankungen des vorderen Augenabschnitts, Essen, Germany. tobias.hudde@uni-essen.de
authors.forename: Tobias
authors.initials: T
authors.lastname: Hudde
authors.forename: Janine
authors.initials: J
authors.lastname: Apitz
authors.forename: Rafael
authors.initials: R
authors.lastname: Bordes-Alonso
authors.forename: Kerstin
authors.initials: K
authors.lastname: Heise
authors.forename: Kristian T M
authors.initials: KT
authors.lastname: Johnson
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Steuhl
authors.forename: Gerd
authors.initials: G
authors.lastname: Geerling
authors.forename: Brigitte M
authors.initials: BM
authors.lastname: Pützer
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000867:Anterior Chamber / Q000201:enzymology / Q000821:virology; D015153:Blotting, Western; D002460:Cell Line; D004727:Endothelium / Q000201:enzymology* / Q000473:pathology; D004728:Endothelium, Corneal / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007267:Injections; D016850:Limbus Corneae / Q000201:enzymology / Q000821:virology; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014129:Trabecular Meshwork / Q000201:enzymology* / Q000473:pathology; D019076:Transgenes
chemical_list: D049452:Green Fluorescent Proteins; D019278:Matrix Metalloproteinase 3