title: Histologic analysis of bioabsorbable scleral buckling implants: an experimental study on rabbits.
abstract: OBJECTIVE
  To analyze histologically tissue reactions to bioabsorbable PLA96 in rabbit eyes.
  
  
  METHODS
  Scleral buckling operations were carried out in 48 rabbits. Two materials were used: bioabsorbable PLA96 (polylactide 96/4; L/D molar ratio 96/4) and silicone sponge. One eye of each rabbit was operated on and the other eye served as a nonoperated control. After follow-up times of 1, 3, 5, and 12 months, the rabbits were killed and the eyes enucleated for histology.
  
  
  RESULTS
  All rabbits recovered well. Histologically, tissue reactions were very localized; implant fragments were not seen within the sclera. The amounts of fibrous tissue and inflammatory cells (mainly macrophages) inside the implant area increased over time. One rabbit from the silicone group was killed 4 months postoperatively owing to refusal to eat. In the PLA96 group, acute or chronic infections occurred in four rabbits. The bioabsorbable implant was macroscopically easily detectable at 12 months postoperatively.
  
  
  CONCLUSIONS
  The PLA96 material used for scleral buckling in rabbits showed good biocompatibility. The material did not undergo biodegradation during the follow-up period of 12 months. PLA96 implants were associated with thicker fibrous tissue encapsulation and more inflammatory cells compared with silicone sponge implants.
authors.affiliation: Department of Ophthalmology, Tampere University of Technology, Finland. satu.lansman@oulu.fi
authors.forename: Satu
authors.initials: S
authors.lastname: Länsman
authors.forename: Paavo
authors.initials: P
authors.lastname: Pääkkö
authors.forename: Jorma
authors.initials: J
authors.lastname: Ryhänen
authors.forename: Heli
authors.initials: H
authors.lastname: Hirvelä
authors.forename: Minna
authors.initials: M
authors.lastname: Kellomäki
authors.forename: Ville
authors.initials: V
authors.lastname: Ellä
authors.forename: Pertti
authors.initials: P
authors.lastname: Törmälä
authors.forename: Timo
authors.initials: T
authors.lastname: Waris
authors.forename: Nureddin A
authors.initials: NA
authors.lastname: Ashammakhi
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials; D005129:Eye Foreign Bodies / Q000473:pathology; D005355:Fibrosis; D005549:Foreign-Body Reaction / Q000473:pathology*; D008264:Macrophages / Q000473:pathology; D008422:Materials Testing; D011091:Polyesters; D019736:Prostheses and Implants; D019919:Prosthesis Implantation; D011817:Rabbits; D012590:Sclera / Q000473:pathology* / Q000601:surgery; D012591:Scleral Buckling / Q000295:instrumentation*; D012826:Silicone Elastomers
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D012826:Silicone Elastomers; C033616:poly(lactide)