Composition of Regenerated Cell Walls of Reverting Aureobasidium pullulans Protoplasts.
Protoplasts of Aureobasidium pullulans, formed by treating normal blastospores with polysaccharide-hydrolyzing enzymes, synthesized glycans when incubated in shallow culture. The polysaccharides formed a loose, thick coating on the cells. The glycans that were formed are similar to the glycans in normal cell walls; however, they lack the branching that is normally found. This result is consistent with the findings of others who have studied the cell wall composition of reverting yeast protoplasts.
Quigley
D R
DR
Chemistry Department, University of Missouri-Rolla, Rolla, Missouri 65401.
Siehr
D J
DJ