Dexamethasone alters vascular reactivity by enhancing COX-related vasodilatation in fetal ovine carotids.
Based on preliminary studies that contractile efficacy was altered in vertebral and basilar arteries from neonatal donors treated with postnatal glucocorticoids, we examined the hypothesis that postnatal dexamethasone (DEX), a glucocorticoid used for respiratory disease in neonates, can alter vascular reactivity. Using near-term fetal lamb carotids, we measured 5-hydroxytryptamine (5-HT) dose-response relationship in DEX-treated and untreated arteries. We found that DEX incubation for 1 h had no effect on 5-HT sensitivity and agonist affinity but significantly reduced 5-HT contractile efficacy, a response that became even more pronounced after 4 h of DEX treatment. Coincubation of DEX-treated arteries with INDO for 4 h reversed this DEX-induced attenuation in 5-HT contractile efficacy, although DEX had no significant effects on cyclooxygenase (COX)-1 and COX-2 protein abundance. This data suggests that DEX alters vascular reactivity through a COX-related mechanism, with possible repercussions to neurological injury.
Angeles
Danilyn M
DM
Department of Physiology, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA. dangeles@llu.edu
Chang
Melody
M
Leong
Valerie
V
Oberg
Kerby C
KC
Pearce
William J
WJ
D000818:Animals; D001488:Basilar Artery / Q000502:physiology; D002339:Carotid Arteries / Q000187:drug effects / Q000502:physiology*; D051545:Cyclooxygenase 1 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D005333:Fetus; D006801:Humans; D007231:Infant, Newborn; D020768:Middle Cerebral Artery / Q000502:physiology; D012756:Sheep; D014664:Vasodilation / Q000502:physiology*
D003907:Dexamethasone; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2