title: [Does sepsis impair the healing of colonic anastomosis in splenectomized rats?].
abstract: BACKGROUND
  To investigate the effect of splenectomy on the healing of colonic anastomoses under normal and septic conditions.
  
  
  METHODS
  Forty Wistar rats were assigned into six groups: group 1: sham, group 2: colonic anastomose, group 3: splenectomy, group 4: colonic anastomose plus sepsis, group 5: colonic anastomose plus splenectomy, group 6: colonic anastomose plus splenectomy plus sepsis. The rats underwent a standardized left colonic resection and primary anastomosis and/or splenectomy. Sepsis was produced by cecal ligation and puncture. Wound healing was evaluated by bursting pressure and hydroxiproline estimates.
  
  
  RESULTS
  Bursting pressures were as follows: group 1: 173 +/- 14 mmHg, group 2: 186 +/- 7 mmHg, group 3: 168 +/- 6 mmHg, group 4: 113 +/- 14 mmHg, group 5: 167 +/- 10 mmHg, and group 6: 183 +/- 3 mmHg. Hidroksiprolin contents were: group 1: 3.5 +/- 0.2 microg/mg, group 2: 3.2 +/- 0.3 microg/mg, group 3: 3.4 +/- 0.2 microg/mg, group 4: 2.3 +/- 0.2 microg/mg, group 5: 3.0 +/- 0.2 microg/mg, grup 6 3.2 +/- 0.1 microg/mg. Statistical significance was found between group 4 and the other groups (p<0.05).
  
  
  CONCLUSIONS
  Sepsis impairs the healing of colonic anastomoses. However, sepsis does not impair the intestinal wound healing in splenectomized rats.
authors.affiliation: Mersin University, Faculty of Medicine Departments of Pediatric Surgery, Mersin, Turkey. anayci@mersin.edu.tr.
authors.forename: Ali
authors.initials: A
authors.lastname: Naycı
authors.forename: Ülkü
authors.initials: Ü
authors.lastname: Çömelekoğlu
authors.forename: Eda
authors.initials: E
authors.lastname: Çıngı
authors.forename: Hakan
authors.initials: H
authors.lastname: Taşkınlar
authors.forename: Dinçer
authors.initials: D
authors.lastname: Avlan
authors.forename: Nurten
authors.initials: N
authors.lastname: Renda
authors.forename: Selim
authors.initials: S
authors.lastname: Aksöyek
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000737:chemistry / Q000601:surgery*; D004195:Disease Models, Animal; D006909:Hydroxyproline / Q000032:analysis; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000503:physiopathology*; D013156:Splenectomy; D000267:Tissue Adhesions; D014945:Wound Healing / Q000502:physiology*
chemical_list: D006909:Hydroxyproline