Quantification of S-adenosyl methionine in microbial cell extracts.
A sensitive method for quantification of S-adenosyl methionine (SAM) in microbial cell extracts was developed and applied to Corynebacterium glutamicum. The method is based on SAM being completely hydrolyzed into (18)O-homoserine when extracted in boiling H(2) (18)O and thus can be clearly distinguished by GC-MS analysis from naturally labeled homoserine present in the cell extract. Additional quantification of the total homoserine pool, representing both SAM and homoserine, via HPLC allows separate determination of both metabolites.
Krömer
Jens Olaf
JO
Biochemical Engineering, Saarland University, Saarbruecken, Germany.
Heinzle
Elmar
E
Wittmann
Christoph
C
D048230:Corynebacterium glutamicum / Q000737:chemistry*; D006714:Homoserine / Q000032:analysis / Q000737:chemistry; D007553:Isotope Labeling; D010103:Oxygen Isotopes / Q000737:chemistry; D012436:S-Adenosylmethionine / Q000032:analysis* / Q000737:chemistry
D010103:Oxygen Isotopes; D006714:Homoserine; D012436:S-Adenosylmethionine