1JNH values show that N1...N3 hydrogen bonds are stronger in dsRNA A:U than dsDNA A:T base pairs.
Here, we show that 1JNH values are on average 0.4 Hz less negative for double-stranded RNA A:U than for DNA A:T base pairs, which, according to existing theory, suggests that RNA N1...N3 hydrogen bond distances are about 0.02 A shorter than those of DNA. Also, there is a statistically relevant trend between 1JNH and 2hDelta13C2 values, which supports the original hypothesis that 2hDelta13C2 values are also sensitive to hydrogen bond distances. Finally, a context dependence is observed for these values, which suggests that hydrogen-bonding and base-stacking interactions are coupled.
Manalo
Marlon N
MN
Department of Biochemistry & Biophysics, Texas A&M University, College Station, Texas 77843-2128, USA.
Kong
Xiangming
X
LiWang
Andy
A
D000225:Adenine / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D006860:Hydrogen Bonding; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D012330:RNA, Double-Stranded / Q000737:chemistry* / Q000378:metabolism; D013936:Thymidine / Q000737:chemistry / Q000378:metabolism; D014529:Uridine / Q000737:chemistry / Q000378:metabolism
D012330:RNA, Double-Stranded; D004247:DNA; D000225:Adenine; D013936:Thymidine; D014529:Uridine