title: An immunological assay for detection and enumeration of thermophilic biomining microorganisms.
abstract: A specific, fast, and sensitive nonradioactive immunobinding assay for the detection and enumeration of the moderate thermophile Thiobacillus caldus and the thermophilic archaeon Sulfolobus acidocaldarius was developed. It employs enhanced chemiluminescence or peroxidase-conjugated immunoglobulins in a dot or slot blotting system and is very convenient for monitoring thermophilic bioleaching microorganisms in effluents from industrial bioleaching processes.
authors.affiliation: Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: A M
authors.initials: AM
authors.lastname: Amaro
authors.forename: K B
authors.initials: KB
authors.lastname: Hallberg
authors.forename: E B
authors.initials: EB
authors.lastname: Lindström
authors.forename: C A
authors.initials: CA
authors.lastname: Jerez