title: Evaluation of the biofilm-forming ability and genetic typing for clinical isolates of Pseudomonas aeruginosa by enterobacterial repetitive intergenic consensus-based PCR.
abstract: Biofilm formation is an important phenotype associated with chronic Pseudomonas aeruginosa infections. In the present study, a total of 48 P. aeruginosa strains isolated from clinical specimens were examined for their biofilm-forming ability using a microtiter plate method. The different biofilm-forming abilities were demonstrated among the strains; however, most strains formed a larger biofilm than strain PAO1, a reference strain. The genetic typing was also carried out by enterobacterial repetitive intergenic consensus-based polymerase chain reaction. Although they were divided into five groups (A to E), most of the strains showing the higher biofilm-forming ability were found to be in groups D and E, suggesting a significant relationship between the biofilm-forming ability and the genetic group.
authors.affiliation: Department of Microbiology, West China School of Basic and Forensic Medical Sciences, Sichuan University, 610041 Chengdu, China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Wen-xiang
authors.initials: WX
authors.lastname: Jia
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Yin
authors.forename: Jian-yu
authors.initials: JY
authors.lastname: Su
authors.forename: Yali
authors.initials: Y
authors.lastname: Kou
authors.forename: Xie
authors.initials: X
authors.lastname: Yi
authors.forename: Sumio
authors.initials: S
authors.lastname: Shinoda
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Miyoshi
mesh_terms: D015373:Bacterial Typing Techniques; D018441:Biofilms / Q000254:growth & development*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics*; D006761:Hospitals; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000502:physiology*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004269:DNA, Bacterial