Alterations in T-cell subset frequency in peripheral blood in obesity.
BACKGROUND
Obesity affects the regulation of immune and inflammatory responses. This study characterizes differences in peripheral blood lymphocyte phenotype in obese humans.


METHODS
Frequencies of lymphocyte subsets among peripheral blood mononuclear cells were compared between 10 obese (BMI > or = 35) and 10 lean subjects, as determined by antibodies directed against cluster differentiation (CD) markers.


RESULTS
Obese patients demonstrated an increased frequency of CD3+CD4+ T-cells (mean difference 12%, P=0.004), a decreased frequency of CD3+CD8+ T-cells (mean difference 9.4%, P=0.016) and an increased frequency of CD3+CD8+CD95+ T-cells (mean difference 13.3%, P=0.032). No other differences among T-cell or monocyte subsets were noted.


CONCLUSIONS
Obesity is associated with alterations in frequencies of peripheral CD4+ and CD8+ T-cells and aberrations in the expression of CD95 among CD8+ T-cells. These data suggest both CD4+ and CD8+ T-cell compartments, as well as the regulation of CD95 expression on CD8+ T-cells, as targets for further study into obesity's effects on the immune system.
O'Rourke
Robert W
RW
Department of Surgery, Oregon Health & Science University, Portland, OR 97239, USA. orourkro@ohsu.edu
Kay
Thomas
T
Scholz
Mark H
MH
Diggs
Brian
B
Jobe
Blair A
BA
Lewinsohn
David M
DM
Bakke
Antony C
AC
D000328:Adult; D015703:Antigens, CD / Q000097:blood*; D015992:Body Mass Index; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D018655:Lymphocyte Count; D008875:Middle Aged; D009767:Obesity, Morbid / Q000097:blood* / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000378:metabolism*
D015703:Antigens, CD