title: Cathepsin E is a specific marker of dysplasia in APC mouse intestine.
abstract: Transcriptional profiling of APC(Min/+) mouse intestinal epithelial tissue has revealed that cathepsin E (catE) manifests high relative expression in adenomas and carcinomas relative to normal epithelium. Real-time RT-PCR data presented previously confirm the presence of catE transcript in APC(Min/+) adenomatous cells compared with samples derived from normal APC(Min/+) and wild-type tissue. At the protein level, strong, highly specific immunohistochemical staining for catE is displayed in dysplastic lesions of APC(Min/+) mice. Using Western immunoblot analyses, it was additionally established that the urine of tumor-bearing mice contains higher levels of the monomeric form of catE than their wild-type counterparts. These results authenticate the relationship between transcript abundance and protein levels in transformed tissue and suggest potential utility for catE as a marker for the inception and progression of intestinal cancers.
authors.affiliation: Walther Cancer Center, Notre Dame, IN 46556, USA.
authors.forename: Lizette
authors.initials: L
authors.lastname: Busquets
authors.forename: Hector
authors.initials: H
authors.lastname: Guillen
authors.forename: Melanie E
authors.initials: ME
authors.lastname: DeFord
authors.forename: Mark A
authors.initials: MA
authors.lastname: Suckow
authors.forename: Rudolph M
authors.initials: RM
authors.lastname: Navari
authors.forename: Francis J
authors.initials: FJ
authors.lastname: Castellino
authors.forename: Mary
authors.initials: M
authors.lastname: Prorok
mesh_terms: D000236:Adenoma / Q000235:genetics* / Q000473:pathology; D000818:Animals; D015153:Blotting, Western; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D020059:Cathepsin E / Q000032:analysis* / Q000096:biosynthesis* / Q000652:urine; D018450:Disease Progression; D017491:Genes, APC; D007150:Immunohistochemistry; D007410:Intestinal Diseases / Q000235:genetics; D007414:Intestinal Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020059:Cathepsin E