title: Outer Membrane Proteins and Lipopolysaccharides in Pathovars of Xanthomonas campestris.
abstract: Variations in the outer membrane proteins (OMPs) and lipopolysaccharides (LPSs) of 54 isolates belonging to 16 different pathovars of Xanthomonas campestris were characterized. OMP samples prepared by sarcosyl extraction of cell walls and LPS samples prepared by proteinase K treatment of sonicated cells were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of 4 M urea. In general, the OMP and LPS profiles within each pathovar were very similar but different from the profiles of other pathovars. Heterogeneity in OMP and LPS profiles was observed within X. campestris pv. campestris, X. campestris pv. translucens, and X. campestris pv. vesicatoria. LPSs were isolated from six X. campestris pathovars, which fell into two major groups on the basis of O antigenicity. The O antigens of X. campestris pv. begoniae, X. campestris pv. graminis, and X. campestris pv. translucens cross-reacted with each other; the other group consisted of X. campestris pv. campestris, X. campestris pv. pelargonii, and X. campestris pv. vesicatoria. A chemical analysis revealed a significant difference between the compositions of the neutral sugars of the LPSs of those two groups; the LPSs of the first group contained xylose and a 6-deoxy-3-O-methyl hexose, whereas the LPSs of the other group lacked both sugars.
authors.affiliation: Department of General Microbiology, University of Helsinki, P.O. Box 41 (Mannerheimintie 172), SF-00014 Helsinki, and Department of Molecular Bacteriology, National Public Health Institute, SF-00300 Helsinki, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Ojanen
authors.forename: I M
authors.initials: IM
authors.lastname: Helander
authors.forename: K
authors.initials: K
authors.lastname: Haahtela
authors.forename: T K
authors.initials: TK
authors.lastname: Korhonen
authors.forename: T
authors.initials: T
authors.lastname: Laakso