Lymphocyte G-protein receptor kinase (GRK)3 mRNA levels in bipolar disorder.
Linkage studies in bipolar disorder were positive for markers in the region of chromosome 22q12.1 including the gene coding for G-protein receptor kinase (GRK)3. Two of six variants of the GRK3 5'-UTR/promoter were reported to be associated with bipolar disorder. GRK3 protein levels in lymphoblastoid cell lines derived from bipolar patients originating from families with linkage to chromosome 22q11 were reported to be decreased compared to those of control subjects and correlated with disease severity. We compared GRK3 mRNA levels in fresh lymphocytes from 31 bipolar patients vs. 26 control subjects, using real-time RT-PCR. No overall difference was found between patients and controls. However, GRK3 mRNA levels were markedly and significantly reduced in the subgroup of patients with no family history of a major psychiatric disorder compared with patients with family history.
Shaltiel
Galit
G
Stanley Research Center, Ben-Gurion University of the Negev, and Mental Health Center, Beersheva, Israel.
Shamir
Alon
A
Levi
Itzhak
I
Bersudsky
Yuly
Y
Agam
Galila
G
D000328:Adult; D001714:Bipolar Disorder / Q000201:enzymology*; D017931:DNA Primers; D005260:Female; D054770:G-Protein-Coupled Receptor Kinase 3; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051552:beta-Adrenergic Receptor Kinases / Q000096:biosynthesis / Q000378:metabolism*
D017931:DNA Primers; D012333:RNA, Messenger; D054770:G-Protein-Coupled Receptor Kinase 3; C517848:GRK3 protein, human; D051552:beta-Adrenergic Receptor Kinases