title: [Enzymatic synthesis of bis(5'-nucleosidyl) tetra- or triphosphates].
abstract: The total fraction of aminoacyl-tRNA synthases from Escherichia coli has been shown to catalyze the synthesis of the bis(5'-nucleosidyl) oligophosphates Ap4AZT, Ap4d4T, Ap43TC, and Ap4ACV, as well as Ap3AZT and Ap3d4T, from [alpha-32P]ATP and the corresponding nucleoside-5'-tri(or di)phosphate. The resulting compounds, characterized by HPLC, are resistant to alkaline phosphatase. Ap4AZT, Ap4d4T, and Ap43TC are formed with approximately equal efficiency, whereas the efficiencies of the synthesis of Ap4ACV, Ap3AZT, and Ap3d4T are three- to fivefold lower. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2005, vol. 31, no. 6; see also http://www.maik.ru.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Skoblov
authors.forename: M V
authors.initials: MV
authors.lastname: Ias'ko
authors.forename: A M
authors.initials: AM
authors.lastname: Murabuldaev
authors.forename: M K
authors.initials: MK
authors.lastname: Kukhanova
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Skoblov
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D000469:Alkaline Phosphatase / Q000737:chemistry; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D015226:Dinucleoside Phosphates / Q000138:chemical synthesis*; D004926:Escherichia coli / Q000201:enzymology
chemical_list: D015226:Dinucleoside Phosphates; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase; D000604:Amino Acyl-tRNA Synthetases