title: Inverse Diauxy in the Yeast Hansenula anomala: Mutants Derepressed for Malic Acid Utilization in the Presence of Glucose.
abstract: Utilization of l-malic acid by yeast strain Hansenula anomala IGC 4380 is subject to glucose repression. Derepressed mutants were obtained with UV light by use of the nonmetabolizable glucose analog 2-deoxyglucose as a selective agent. Three mutant strains degraded l-malic acid in the presence of up to 30% (wt/vol) glucose and are of potential interest for the biological deacidification of grape must. The mutant strains, as compared with the parent strain, displayed inverse diauxy in glucose-malate medium, glucose being metabolized only after malate consumption had been completed.
authors.affiliation: Laboratory of Biology, University of Minho, 4719 Braga Codex, and Laboratory of Microbiology, Gulbenkian Institute of Science, 2781 Oeiras Codex, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Côrte-Real
authors.forename: C
authors.initials: C
authors.lastname: Leão
authors.forename: N
authors.initials: N
authors.lastname: Van Uden