Helium-neon and nitrogen laser irradiation accelerates the phagocytic activity of human monocytes.
OBJECTIVE
Intracellular survival of mycobacteria within monocytes is a crucial stage in the pathogenesis of tuberculosis. The aim was to check intracellular survival of Mycobacterium fortuitum within the human monocytes exposed to He-Ne and nitrogen laser irradiation.


BACKGROUND
Tuberculosis remains one of the most important infectious diseases for developing countries. Low-level laser therapy (LLLT) has been tried to treat tubercular cavitory lung disease with encouraging results. The in vitro photobiological effect of low level laser radiation on the intracellular mycobacteria needs to be evaluated before we could go for large clinical trials.


METHODS
The aliquots of human monocytes from peripheral blood of healthy volunteers and tuberculosis cases were exposed to He-Ne or nitrogen laser beam. The non-irradiated monocytes from the same source served as controls. The monocytes were then challenged with M. fortuitum, and surviving mycobacteria within monocytes were subjected to viable counts.


RESULTS
Enhanced killing of mycobacterial cells was seen among monocytes exposed to He-Ne and nitrogen laser irradiation.


CONCLUSIONS
He-Ne and nitrogen laser irradiation activates the monocytes to increase intracellular killing of mycobacteria.
Hemvani
Nanda
N
Department of Microbiology and Immunology, Choithram Hospital & Research Centre, Indore, India.
Chitnis
Dhananjay Sadashiv
DS
Bhagwanani
Nijram Satramdas
NS
D006371:Helium; D006801:Humans; D007834:Lasers; D009000:Monocytes / Q000382:microbiology*; D019907:Mycobacterium fortuitum / Q000528:radiation effects*; D009356:Neon; D009584:Nitrogen; D010587:Phagocytosis / Q000528:radiation effects*
D006371:Helium; D009356:Neon; D009584:Nitrogen