Detection of beta-Glucosidase Activity in Polyacrylamide Gels with Esculin as Substrate.
beta-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by beta-glucosidase action reacts with ferric ion to produce a black band, corresponding to the beta-glucosidase, against the transparent background.
Kwon
K S
KS
Department of Microbiology, Seoul National University, Seoul, 151-742, Korea.
Lee
J
J
Kang
H G
HG
Hah
Y C
YC