title: The adrenal gland is a source of stress-induced circulating IL-18.
abstract: The present study compared plasma IL-18 levels between sham-operated and adrenalectomized mice following stress to investigate whether the adrenal gland contributes to the elevation of circulating IL-18 during stress. Two hours of stress provoked a robust, stressor-dependent, elevation of IL-18 mRNA and peptide in the adrenal gland in sham-operated mice. Consistently, levels of circulating mature IL-18 increased during stress and remained elevated for up to 6 h after stress. The stress-induced increase in circulating IL-18 was abolished by adrenalectomy. These findings demonstrate that the adrenal gland is required to achieve elevation of circulating IL-18 during stress.
authors.affiliation: Department of Physiology, Nippon Medical School, 1-1-5 Sendagi Bunkyo-ku, Tokyo 113-8602, Japan. sugama@nms.ac.jp
authors.forename: Shuei
authors.initials: S
authors.lastname: Sugama
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Noriaki
authors.initials: N
authors.lastname: Shimokawa
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Koibuchi
authors.forename: Masayo
authors.initials: M
authors.lastname: Fujita
authors.forename: Makoto
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Firdaus S
authors.initials: FS
authors.lastname: Dhabhar
authors.forename: Bruno
authors.initials: B
authors.lastname: Conti
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism*; D000315:Adrenalectomy / Q000379:methods; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology; D007101:Immersion / Q000009:adverse effects; D007150:Immunohistochemistry / Q000379:methods; D016209:Interleukin-8 / Q000097:blood* / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000096:biosynthesis; D011863:Radioimmunoassay / Q000379:methods; D012149:Restraint, Physical / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013312:Stress, Physiological / Q000378:metabolism*; D013997:Time Factors
chemical_list: D016209:Interleukin-8; D012333:RNA, Messenger; D000324:Adrenocorticotropic Hormone