Biotransformation of 6beta-santonin by Phytolacca acinosa cell suspension cultures.
OBJECTIVE
To obtain more valuable derivatives for the further structural modification of 6beta-santonin (1) via biotransformation by using cell suspension cultures of Phytolacca acinosa.


METHODS
The substrate 1 was incubated with cell suspension cultures of P. acinosa, the products were obtained by chromatography, and identified on the basis of their physical and spectral data (HRMS, 1D NMR, 2D NMR, NOE and IR).


RESULTS
After incubation with cell suspension cultures of P. acinosa, 1 was converted into five products. Among them, 3 is a new compound.


CONCLUSIONS
6beta-santonin could be selectively reduced and hydroxylated by the cell suspension cultures of P. acinosa, which would provide valuable intermediates for its further structural modification.
Yang
Lin
L
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Dai
Jun-Gui
JG
D001711:Biotransformation; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D015394:Molecular Structure; D029603:Phytolacca / Q000166:cytology / Q000378:metabolism*; D010946:Plants, Medicinal / Q000166:cytology / Q000378:metabolism*; D012500:Santonin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*
D012500:Santonin