Structural analysis of kappa-carrageenan [corrected] sulfated oligosaccharides by positive mode nano-ESI-FTICR-MS and MS/MS by SORI-CID.
Structural analysis of sulfated oligosaccharides from kappa-carrageenan of up to ten residues (MW >2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.
Aguilan
Jennifer T
JT
Department of Chemistry, Ateneo de Manila University, Quezon City, Philippines.
Dayrit
Fabian M
FM
Zhang
Jinhua
J
Niñonuevo
Milady R
MR
Lebrilla
Carlito B
CB
D002240:Carbohydrate Sequence; D002351:Carrageenan / Q000737:chemistry*; D005583:Fourier Analysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization; D013431:Sulfates / Q000737:chemistry
D009844:Oligosaccharides; D013431:Sulfates; D002351:Carrageenan