title: In Vitro Growth of Acremonium coenophialum, an Endophyte of Toxic Tall Fescue Grass.
abstract: Acremonium coenophialum, an endophytic fungus present in toxic tall fescue grass and seed, grew very slowly or not at all with conventional media and cultural practices. However, a considerable increase in growth was achieved in a relatively dilute medium consisting solely of glucose and yeast extract. The optimal levels of glucose and yeast extract were 3 to 6% and 0.35% (wt/vol), respectively. The addition of salts which lowered the pH suppressed growth. Even when the pH was controlled, the addition of KH(2)PO(4) at a level of 3.2% or more greatly inhibited growth. A. coenophialum grew better in shake culture than in stationary culture. The optimal temperature was 23 degrees C, and the optimal pH was 6.5.
authors.affiliation: Department of Botany, Plant Pathology, and Microbiology, Alabama Agricultural Experiment Station, Auburn University, Auburn, Alabama 36849.
authors.forename: N D
authors.initials: ND
authors.lastname: Davis
authors.forename: E M
authors.initials: EM
authors.lastname: Clark
authors.forename: K A
authors.initials: KA
authors.lastname: Schrey
authors.forename: U L
authors.initials: UL
authors.lastname: Diener