title: Calculation of k and v from substrate concentration versus time plot.
abstract: A simple method for the calculation of kinetic parameters (K(m), V(max)) under conditions of changing substrate concentrations is presented. An application of the method to detect shifts in groups involved in the utilization of a substrate in a mixed microbial culture is given.
authors.affiliation: Laboratory of Animal Nutrition, State University of Utrecht, Yalelaan 1, Utrecht, The Netherlands.
authors.forename: G H
authors.initials: GH
authors.lastname: Counotte
authors.forename: R A
authors.initials: RA
authors.lastname: Prins