Cyclooxygenase 1 and 2 expressions in the human thyroid gland.
We investigated the expression of COX-1 and COX-2 in normal thyroid tissue, follicular adenoma and well-differentiated thyroid carcinomas and evaluated the difference in COX-1 and COX-2 expression. Ten normal thyroid tissues, ten follicular adenomas, ten papillary carcinomas and ten follicular carcinomas were analyzed by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) for expression of COX-1 and COX-2 mRNA. In addition, immunohistochemical staining was performed to find the expression of the two enzymes in normal thyroid tissues and thyroid neoplasia. Expression of COX-1 mRNA in the normal thyroid tissues, follicular adenomas and both well-differentiated carcinomas was similar and weak. However, COX-2 mRNA was strongly expressed in the well-differentiated carcinomas compared to those of normal thyroid tissue and follicular adenoma.
Lee
Heung-Man
HM
Department of Otolaryngology and Head and Neck Surgery, Korea University College of Medicine, Guro Hospital, 97 Gurodong-gil Guro-gu, 152-703, Seoul, Korea.
Baek
Seung-Kuk
SK
Kwon
Soon-Young
SY
Jung
Kwang-Yoon
KY
Chae
Sung-Won
SW
Hwang
Soon-Jae
SJ
Woo
Jeong-Soo
JS
Lee
June-Young
JY
D000236:Adenoma / Q000235:genetics / Q000473:pathology; D014408:Biomarkers, Tumor; D001706:Biopsy; D002291:Carcinoma, Papillary / Q000235:genetics / Q000473:pathology; D051545:Cyclooxygenase 1 / Q000235:genetics* / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013961:Thyroid Gland / Q000378:metabolism* / Q000473:pathology; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2