title: Determination of theophylline concentration in serum by chemiluminescent immunoassay.
abstract: OBJECTIVE
  This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum.
  
  
  METHODS
  To measure the concentration of theophylline (n=122) and evaluate the assay.
  
  
  RESULTS
  The linear range of the CLIA method was 0.51-40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 micromol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L).
  
  
  CONCLUSIONS
  This method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.
authors.affiliation: Department of Laboratory, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China. zmx00018@126.com
authors.forename: Mei-xia
authors.initials: MX
authors.lastname: Zhou
authors.forename: Cha-ying
authors.initials: CY
authors.lastname: Guan
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.forename: Xin-you
authors.initials: XY
authors.lastname: Xie
authors.forename: Sheng-hai
authors.initials: SH
authors.lastname: Wu
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D005260:Female; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008171:Lung Diseases / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013806:Theophylline / Q000097:blood*
chemical_list: D013806:Theophylline