[Determination of the conversion of air oxidation of penicillin derivatives by high performance liquid chromatography].
In air oxidation of penicillin G p-methoxybenzyl ester (PGPMB) to its sulfoxides (PGPMBO), sol-gel technique was employed to encapsulate the catalyst Co (acac)3, by which the reaction was run under heterogeneous conditions. A reversed-phase high performance liquid chromatographic method was established for the determination of the conversion of this reaction. The analysis of PGPMBO was carried out on a C18 column (4.6 mm i.d. x 150 mm, 10 microm) with the mobile phase of methanol-water (85:15, V/V), at a flow rate of 1 mL min(-1) and with detection wavelength of 270 nm. The linearity of the calibration curve of PGPMBO was obtained over the range of 0.05 g x L(-1) - 0.40 g x L(-1) with a relative coefficient of 0.999 2. The highest conversion was 98.8%. The method can be applied to determine this conversion promptly in the reaction system.
Hu
Qiu-Yi
QY
Department of Pharmaceutical Engineering, School of Chemical Engineering, Hebei University of Technology, Tianjin 300130, China. huqiuyi@eyou.com
Zhao
Ji-Quan
JQ
Zhu
Ling-Di
LD
Li
Meng-Qing
MQ
Li
Guo-Hua
GH
Wang
Yang
Y
Ge
Feng-Yan
FY
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D010084:Oxidation-Reduction; D010400:Penicillin G / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D010400:Penicillin G