Induction of RANTES and CCR5 through NF-kappaB activation via MAPK pathway in aged rat gingival tissues.
Chemokine and chemokine receptor expression in gingival tissues plays a central role in periodontal disease during aging. In the present study, we explored the modulation of chemokines and chemokine receptors expression in aging rat gingival tissues. In the 24-month-old (Old) rat gingival tissues, RANTES and CCR5 mRNA and protein levels were 2-4 fold increased over those of the 6-month-old (Young) rats. The Old rats had considerable enhancement of all three of the studied MAPK activities: extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 MAPK. These results suggest that age-related increases in RANTES and CCR5 expression are associated with increased IkappaBalpha, nuclear NF-kappaB, and MAPK activity in gingival tissues.
Kim
Hyung Keun
HK
Research Institute of Genetic Engineering, Pusan National University, 609-735, Busan, Korea.
Park
Hae Ryoun
HR
Sul
Kyoung Hee
KH
Chung
Hae Young
HY
Chung
Jin
J
D000375:Aging / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D018946:Chemokine CCL5 / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D005881:Gingiva / Q000378:metabolism*; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D019713:Receptors, CCR5 / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018946:Chemokine CCL5; D016328:NF-kappa B; D011506:Proteins; D012333:RNA, Messenger; D019713:Receptors, CCR5; D020928:Mitogen-Activated Protein Kinases