title: Characterization of Peroxidase Secretion and Subcellular Organization of Phanerochaete chrysosporium INA-12 in the Presence of Various Soybean Phospholipid Fractions.
abstract: Stimulation of lignin peroxidase production by exogenous phospholipids depends on the composition of the phospholipid fraction prepared by using the Nattermann process. The fraction composed mainly of negatively charged phospholipids (NAT 89) was the most efficient source for exoprotein secretion by Phanerochaete chrysosporium INA-12. The results of biochemical marker assays and ultrastructural morphology determination by electron microscopy were correlated. Activities of succinate dehydrogenase, a mitochondrial marker, and cytochrome c oxidoreductase, an endoplasmic reticulum (ER) marker, were increased 1.3- and 2.2-fold, respectively, in the presence of NAT 89. Electron microscopy observations suggested that the amount of mitochondria and ER in culture containing phospholipids was increased at the optimum day of lignin peroxidase production. Therefore, phospholipids enhanced energetic metabolism of strain INA-12 and markedly modified fungus physiology. Since ER is involved in enzyme synthesis, we suggest that its increased amount in mycelium cultured with NAT 89 is directly associated with the higher production of lignin peroxidase.
authors.affiliation: Laboratoire de Génie des Procédés Biotechnologiques Agro-Alimentaires, Centre de Biotechnologies Agro-Industrielles, Institut National de la Recherche Agronomique, 78850 Thiverval-Grignon, Rhône-Poulenc Santé, Centre de Recherche de Vitry-Alfortville, 94403 Vitry sur Seine, and Service de Microscopie, Centre National de la Recherche Agronomique, 78000 Versailles, France, and Nattermann Phospholipid GmbH, D-5000 Cologne, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Capdevila
authors.forename: S
authors.initials: S
authors.lastname: Moukha
authors.forename: M
authors.initials: M
authors.lastname: Ghyczy
authors.forename: J
authors.initials: J
authors.lastname: Theilleux
authors.forename: B
authors.initials: B
authors.lastname: Gelie
authors.forename: M
authors.initials: M
authors.lastname: Delattre
authors.forename: G
authors.initials: G
authors.lastname: Corrieu
authors.forename: M
authors.initials: M
authors.lastname: Asther