Nitric oxide inhibition of the proliferation of ovarian endometriotic stromal cells in vitro.
OBJECTIVE
To investigate the effects of nitric oxide on endometrial cell proliferation and the effects of peritoneal fluid from women with and without endometriosis on the production of nitric oxide and upon the nature of nitric oxide-induced changes.


METHODS
Ovarian endometriotic and endometrial stromal cells and peritoneal fluid were obtained from endometriosis patients and controls. Cell proliferation was determined by [3H]thymidine incorporation, and nitrite was measured using Griess reagent.


RESULTS
Sodium nitroprusside (SNP), a nitric oxide donor, reduced the proliferation of endometriotic and endometrial cells in primary culture in a dose-dependent manner. SNP-induced production of nitric oxide and the inhibitory effect of nitric oxide on stromal cell proliferation were reduced by peritoneal fluid from women with endometriosis, although stromal cell proliferation was still inhibited by SNP in the presence of peritoneal fluid. However, this SNP-induced inhibition of cell proliferation was unaffected by interleukin-8, 17-beta, estradiol or transforming growth factor beta1.


CONCLUSIONS
Nitric oxide inhibits the proliferation of endometrial stromal cells in vitro, and this inhibitory effect is abrogated by peritoneal fluid from women with endometriosis.
Kim
Ki Hyung
KH
Department of Obstetrics and Gynecology, College of Medicine, Pusan National University, Busan, Republic of Korea.
Kwak
Jong Young
JY
Shin
Byung Sub
BS
Choi
Young Min
YM
Oh
Sung Tack
ST
Lee
Kyu Sup
KS
D001202:Ascitic Fluid / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured / Q000502:physiology; D003470:Culture Media; D016207:Cytokines / Q000502:physiology; D004715:Endometriosis / Q000503:physiopathology*; D004717:Endometrium / Q000166:cytology*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D009569:Nitric Oxide / Q000502:physiology*; D009599:Nitroprusside / Q000494:pharmacology; D010053:Ovary / Q000166:cytology; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology
D003470:Culture Media; D016207:Cytokines; D009599:Nitroprusside; D009569:Nitric Oxide