title: Artificial transcription factors increase production of recombinant antibodies in Chinese hamster ovary cells.
abstract: A randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production approximately 10-fold at approximately 60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells.
authors.affiliation: ToolGen, Inc. Daejeon Bio-Venture Town, 461-58, Jeonmin-dong, 305-390, Yuseong-gu, Daejeon, South Korea.
authors.forename: Ryuk-Jun
authors.initials: RJ
authors.lastname: Kwon
authors.forename: Seong Keun
authors.initials: SK
authors.lastname: Kim
authors.forename: Seung-Il
authors.initials: SI
authors.lastname: Lee
authors.forename: Su-Jeong
authors.initials: SJ
authors.lastname: Hwang
authors.forename: Gyun Min
authors.initials: GM
authors.lastname: Lee
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Wongi
authors.initials: W
authors.lastname: Seol
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D005822:Genetic Vectors; D006801:Humans; D019151:Peptide Library; D011994:Recombinant Proteins / Q000096:biosynthesis; D012190:Retroviridae / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D019151:Peptide Library; D011994:Recombinant Proteins; D014157:Transcription Factors