Quantum dot-based multiplexed fluorescence resonance energy transfer.
We demonstrate the use of luminescent quantum dots (QDs) conjugated to dye-labeled protein acceptors for nonradiative energy transfer in a multiplexed format. Two configurations were explored: (1) a single color QD interacting with multiple distinct acceptors and (2) multiple donor populations interacting with one type of acceptor. In both cases, we showed that simultaneous energy transfer between donors and proximal acceptors can be measured. However, data analysis was simpler for the configuration where multiple QD donors are used in conjunction with one acceptor. Steady-state fluorescence results were corroborated by time-resolved measurements where selective shortening of QD lifetime was measured only for populations that were selectively engaged in nonradiative energy transfer.
Clapp
Aaron R
AR
U.S. Naval Research Laboratory, Optical Sciences Division, Code 5611, Washington, DC 20375, USA.
Medintz
Igor L
IL
Uyeda
H Tetsuo
HT
Fisher
Brent R
BR
Goldman
Ellen R
ER
Bawendi
Moungi G
MG
Mattoussi
Hedi
H
D002352:Carrier Proteins / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D058619:Maltose-Binding Proteins; D045663:Quantum Dots
D002352:Carrier Proteins; D005456:Fluorescent Dyes; D058619:Maltose-Binding Proteins