title: Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies.
abstract: OBJECTIVE
  To test a newly invented intracytoplasmic sperm injection (ICSI) sperm selection method based on sperm hyaluronic acid (HA) binding.
  
  
  METHODS
  Comparison of chromosomal disomy and diploidy frequencies in sperm arising from semen and in HA-bound sperm.
  
  
  METHODS
  Academic andrology laboratory.
  
  
  METHODS
  Men presenting for semen analysis.
  
  
  METHODS
  Washed sperm fractions of 32 semen samples were applied to Petri dishes or glass slides coated with immobilized HA. The unbound sperm were rinsed gently, and the HA-bound sperm were removed with an ICSI pipette. The control sperm population was the unselected sperm. Both HA-selected and unselected sperm were treated with fluorescence in situ hybridization with centromeric probes for the X, Y, and 17 chromosomes.
  
  
  METHODS
  Chromosomal disomy and diploidy frequencies.
  
  
  RESULTS
  In the HA-bound sperm (495-2,079 per man, 41,670 in all) compared with unselected sperm (4,770 per man, 162,210 in all), the chromosomal disomy frequencies were reduced to 0.16% from 0.52%, diploidy to 0.09% from 0.51%, and sex chromosome disomy to 0.05% from 0.27% (a 5.4-fold reduction vs. 4-fold respective increase in ICSI offspring).
  
  
  CONCLUSIONS
  The HA sperm selection method for ICSI, which is based on a relationship between sperm receptors for zona pellucida and HA, will likely reduce the potential genetic complications and adverse public health effects of ICSI.
authors.affiliation: Sperm Physiology Laboratory, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
authors.forename: Attila
authors.initials: A
authors.lastname: Jakab
authors.forename: Denny
authors.initials: D
authors.lastname: Sakkas
authors.forename: Elena
authors.initials: E
authors.lastname: Delpiano
authors.forename: Sevil
authors.initials: S
authors.lastname: Cayli
authors.forename: Ertug
authors.initials: E
authors.lastname: Kovanci
authors.forename: David
authors.initials: D
authors.lastname: Ward
authors.forename: Alberto
authors.initials: A
authors.lastname: Revelli
authors.forename: Alberto
authors.initials: A
authors.lastname: Ravelli
authors.forename: Gabor
authors.initials: G
authors.lastname: Huszar
mesh_terms: D033441:Andrology / Q000379:methods; D000782:Aneuploidy; D004171:Diploidy; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D066298:In Vitro Techniques; D008297:Male; D009845:Oligospermia / Q000473:pathology* / Q000628:therapy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism; D024182:Uniparental Disomy / Q000473:pathology
chemical_list: D006820:Hyaluronic Acid