Fast chromatin immunoprecipitation assay.
Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol-chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples. We have developed an efficient and rapid Chelex resin-based ChIP procedure that dramatically reduces time of the assay and uses only a single tube to isolate PCR-ready DNA. This method greatly facilitates the probing of chromatin changes over many time points with several antibodies in one experiment.
Nelson
Joel D
JD
Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98109, USA.
Denisenko
Oleg
O
Sova
Pavel
P
Bomsztyk
Karol
K
D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000737:chemistry*; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D051381:Rats; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013997:Time Factors
D002843:Chromatin; D004247:DNA