An efficient protocol for genetic transformation and shoot regeneration of turmeric (Curcuma longa L.) via particle bombardment.
Turmeric (Curcuma longa L.) is an important spice crop plant that is sterile and cannot be improved by conventional breeding. An efficient method for stable transformation for turmeric, C. longa L., was developed using particle bombardment. Callus cultures initiated from shoots were bombarded with gold particles coated with plasmid pAHC25 containing the bar and gusA genes each driven by the maize ubiquitin promoter. Transformants were selected on medium containing glufosinate. Transgenic lines were established on selection medium from 50% of the bombarded calluses. Transgenic shoots regenerated from these were multiplied and stably transformed plantlets were produced. Polymerase chain reaction (PCR) and histochemical GUS assay confirmed the stable transformation. Transformed plantlets were resistant to glufosinate.
Shirgurkar
Mrudul V
MV
Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, Pune 411008, India.
Naik
Vaishali B
VB
von Arnold
Sara
S
Nadgauda
Rajani S
RS
Clapham
David
D
D000613:Aminobutyrates / Q000494:pharmacology; D019470:Biolistics; D018929:Cell Culture Techniques; D030024:Curcuma / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D004351:Drug Resistance / Q000187:drug effects; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering / Q000379:methods*; D006540:Herbicides / Q000494:pharmacology; D010315:Particle Accelerators; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development*; D030821:Plants, Genetically Modified; D012038:Regeneration; D014170:Transformation, Genetic
D000613:Aminobutyrates; D006540:Herbicides; C003121:phosphinothricin