title: Multivariate statistical tools applied to the characterization of the proteomic profiles of two human lymphoma cell lines by two-dimensional gel electrophoresis.
abstract: Mantle cell lymphoma (MCL) cell lines have been difficult to generate, since only few have been described so far and even fewer have been thoroughly characterized. Among them, there is only one cell line, called GRANTA-519, which is well established and universally adopted for most lymphoma studies. We succeeded in establishing a new MCL cell line, called MAVER-1, from a leukemic MCL, and performed a thorough phenotypical, cytogenetical and molecular characterization of the cell line. In the present report, the phenotypic expression of GRANTA-519 and MAVER-1 cell lines has been compared and evaluated by a proteomic approach, exploiting 2-D map analysis. By univariate statistical analysis (Student's t-test, as commonly used in most commercial software packages), most of the protein spots were found to be identical between the two cell lines. Thirty spots were found to be unique for the GRANTA-519, whereas another 11 polypeptides appeared to be expressed only by the MAVER-1 cell line. A number of these spots could be identified by MS. These data were confirmed and expanded by multivariate statistical tools (principal component analysis and soft-independent model of class analogy) that allowed identification of a larger number of differently expressed spots. Multivariate statistical tools have the advantage of reducing the risk of false positives and of identifying spots that are significantly altered in terms of correlated expression rather than absolute expression values. It is thus suggested that, in future work in differential proteomic profiling, both univariate and multivariate statistical tools should be adopted.
authors.affiliation: Department of Environmental and Life Sciences, University of Eastern Piedmont, Alessandria, Italy. marengoe@tin.it
authors.forename: Emilio
authors.initials: E
authors.lastname: Marengo
authors.forename: Elisa
authors.initials: E
authors.lastname: Robotti
authors.forename: Marco
authors.initials: M
authors.lastname: Bobba
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Liparota
authors.forename: Chiara
authors.initials: C
authors.lastname: Rustichelli
authors.forename: Alberto
authors.initials: A
authors.lastname: Zamò
authors.forename: Marco
authors.initials: M
authors.lastname: Chilosi
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
mesh_terms: D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D020522:Lymphoma, Mantle-Cell; D015999:Multivariate Analysis; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome