title: Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering.
abstract: The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFlFFF-MALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (d(H)) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum d(H) was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Luo
authors.forename: Mats
authors.initials: M
authors.lastname: Leeman
authors.forename: Andras
authors.initials: A
authors.lastname: Ballagi
authors.forename: Anders
authors.initials: A
authors.lastname: Elfwing
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Su
authors.forename: Jan-Christer
authors.initials: JC
authors.lastname: Janson
authors.forename: Karl-Gustav
authors.initials: KG
authors.lastname: Wahlund
mesh_terms: D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D031521:Fractionation, Field Flow / Q000295:instrumentation / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D002479:Inclusion Bodies / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008027:Light; D008970:Molecular Weight; D012542:Scattering, Radiation; D013997:Time Factors
chemical_list: D049452:Green Fluorescent Proteins; D007544:Isopropyl Thiogalactoside