Pressure as a tool to study protein-unfolding/refolding processes: the case of ribonuclease A.
This paper gives an overview of the application of high-pressure to study the folding/unfolding processes of proteins using Ribonuclease A as a model protein. A particular focus is the study of pressure-equilibrium unfolding and folding kinetics using variants and the information obtained by comparing these with the wild-type enzyme.
Ribó
M
M
Laboratori d'Enginyeria de Proteïnes, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Campus de Montilivi s/n, 17071 Girona, Spain.
Font
J
J
Benito
A
A
Torrent
J
J
Lange
R
R
Vilanova
M
M
D000818:Animals; D006801:Humans; D011312:Pressure; D011487:Protein Conformation; D017510:Protein Folding; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000235:genetics
D012259:Ribonuclease, Pancreatic