Efficacy of polyethylene glycols in University of Wisconsin preservation solutions: a study of isolated perfused rat liver.
Recent reports argue that the performance of University of Wisconsin (UW) solution is limited by the presence of hydroxyethyl starch (HES) as an additive, since HES could be responsible for human red blood cell aggregation. We investigated the effect on rat liver preservation of replacing HES in UW solution by polyethylene glycols (PEG20 and PEG35) at two concentrations. An isolated perfused rat liver model was used. Six groups of preserved livers (n = 7 for each group) were compared to controls (nonpreserved livers, n = 7). The following preservation solutions were assayed: UW without oncotic supply, UW-HES (0.25 mmol/L), UW-PEG20 (0.03 and 0.25 mmol/L), and UW-PEG35 (0.03 and 0.25 mmol/L). After 24-hour cold storage, the livers were perfused for 120 minutes at 37 degrees C with oxygenated Krebs-Henseleit solution. During perfusion, transaminase release, portal and bile flows, and bromosulfophthalein (BSP) clearance were assessed. Results showed that the omission of oncotic supply in UW statistically increased ALT and AST release in perfusate and decreased bile and portal flows. PEG addition in UW solution, especially PEG35 at 0.25 mmol/L, effectively protected the rat liver graft from the onset of hypothermic ischemia/reperfusion damage. In conclusion, data reported here reveal that oncotic supply is essential for liver preservation and that HES can be effectively replaced by PEG in UW solution.
Mosbah
I Ben
IB
Departamento de Patología Experimental, Instituto de Investigaciones Biomédicas de Barcelona, Spain.
Saidane
D
D
Peralta
C
C
Roselló-Catafau
J
J
Abdennebi
H Ben
HB
D000241:Adenosine; D000410:Alanine Transaminase / Q000097:blood; D000493:Allopurinol; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001646:Bile / Q000378:metabolism; D005978:Glutathione; D007328:Insulin; D008099:Liver / Q000187:drug effects / Q000502:physiology*; D008111:Liver Function Tests; D008297:Male; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011168:Portal System / Q000187:drug effects / Q000502:physiology; D011887:Raffinose; D051381:Rats; D017207:Rats, Sprague-Dawley
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D011092:Polyethylene Glycols; D000493:Allopurinol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose