Immunoassays based on microelectrodes arrayed on a silicon chip for high throughput screening of liver fibrosis markers in human serum.
A novel immunoassays for screening of disease markers in human serum are presented by miniaturizing interdigitated array (IDA) of microelectrodes via micro electro-mechanical system (MEMS) on a silicon chip for multi-channel electrochemical measurement. Different selected antibodies (Abs) are incorporated site-specifically into the electrochemically deposited polypyrrole (PPy) formed on the IDA of the silicon chip, which was characterized by fluorescence microscope photo and the electrochemical quartz crystal microbalance (EQCM) measurements. The selective recognition of Ab to the corresponding antigen (Ag) is monitored through the measurable conductivity change, which is directly visualized by cyclic voltammograms (CVs) in presence of the redox probe, Fe (CN)6(3-/4-). By using the strategy presented here, three liver fibrosis markers, hyaluronic acid (HA), lamin (LN) and collagen type IV (IV-C), are detected simultaneously and specifically at the surface of the chip with calibration curves, y = 21.75 + 0.84x (R = 0.995), y = 57.54 + 0.47x (R = 0.999) and y = 37.92 + 0.28x (R = 0.999), separately. Either the standard or the serum samples can be detected at ng/mL concentration level in a tiny amount of volume, approximately 50 microL. The chip-based immunoassay shows the advantages of high sensitivity, good specificity, high throughput, low sample consumption, and the stability offered via batch production by MEMS as well, which is expected to benefit the multi-target screening of desired clinical analytes.
Shi
Mianhong
M
Chemistry Department, Fudan University, Shanghai 200433, China.
Peng
Youyuan
Y
Zhou
Jia
J
Liu
Baohong
B
Huang
Yipin
Y
Kong
Jilie
J
D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D001798:Blood Proteins / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005355:Fibrosis / Q000097:blood* / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008839:Microelectrodes; D008904:Miniaturization; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D012825:Silicon
D015415:Biomarkers; D001798:Blood Proteins; D012825:Silicon