Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods.
Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates from a woman with unknown HIV status, had been identified as belonging to the Mycobacterium avium complex by conventional procedures. In both patients, using genetic analysis procedures such as PCR-restriction enzyme analysis (PRA) of the hsp65 gene, a commercially available reverse hybridization-based assay (INNO-LiPA mycobacteria) and/or sequencing analysis of the 16S-23S internal transcribed spacer (ITS), the presence of Mycobacterium lentiflavum was also demonstrated. At the time of detection, both cases were also infected with M. avium, suggesting an underestimation of infection with M. lentiflavum and co-infection with different Mycobacterium species.
Suffys
Philip
P
da Silva Rocha
Adalgiza
A
Brandão
Adeilton
A
Vanderborght
Bart
B
Mijs
Wouter
W
Innogenetics N. V., Technologiepark 6, B-9052 Ghent, Belgium.
Jannes
Geert
G
Innogenetics N. V., Technologiepark 6, B-9052 Ghent, Belgium.
Mello
Fernanda C Q
FCQ
Pedro
Heloisa da Silveira Paro
HDSP
Adolfo Lutz Institute, São Paulo, Brazil.
de Souza Fonseca
Leila
L
de Oliveira
Rosângela Siqueira
RS
Department of Microbiology, Immunology and Parasitology, Paulista School of Medicine, São Paulo, Brazil.
Cardoso Leão
Sylvia
S
Department of Microbiology, Immunology and Parasitology, Paulista School of Medicine, São Paulo, Brazil.
Féres Saad
Maria Helena
MH
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics; D001769:Blood / Q000382:microbiology; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005821:Genetic Techniques; D005838:Genotype; D015658:HIV Infections / Q000150:complications; D006801:Humans; D008168:Lung / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000150:complications* / Q000382:microbiology; D015269:Mycobacterium avium Complex / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015270:Mycobacterium avium-intracellulare Infection / Q000150:complications* / Q000382:microbiology
D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins