DNA triplex stabilization by a delta-carboline derivative tethered to third strand oligonucleotides.
A delta-carboline derivative was covalently coupled to a 7 mer oligonucleotide at its 5'- or 3'-end. The stability of triplexes formed from the conjugates and a double-helical target was studied by UV melting experiments. Compared to the unmodified control triple helices, triplexes with the conjugate exhibit a significantly higher stability. However, the degree of stabilization depends on the particular triplex structure formed.
Todorović
Nina
N
Institut für Chemie und Biochemie, Ernst-Moritz-Arndt-Universität Greifswald, Soldmannstrasse 16, D-17489 Greifswald, Germany.
Phuong
Nguyen Thi Bich
NT
Langer
Peter
P
Weisz
Klaus
K
D020029:Base Pairing; D002243:Carbolines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry* / Q000528:radiation effects; D009841:Oligonucleotides / Q000737:chemistry*; D013696:Temperature; D014466:Ultraviolet Rays
D002243:Carbolines; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; C051802:triplex DNA; D004247:DNA