Safe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity.
Concern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist.
Warren
Robin
R
DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa. rw1@sun.ac.za
de Kock
Marianna
M
Engelke
Erica
E
Myburgh
Roseline
R
Gey van Pittius
Nico
N
Victor
Thomas
T
van Helden
Paul
P
D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000097:blood / Q000302:isolation & purification*; D006358:Hot Temperature; D006801:Humans; D007757:Laboratory Infection / Q000517:prevention & control*; D009164:Mycobacterium Infections / Q000175:diagnosis; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D017751:Safety Management / Q000379:methods
D004269:DNA, Bacterial