Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes.
The Moloney murine leukemia virus-transformed suspension cell line WMP2 is derived from wild mice (Mus musculus) of the WMP/WMP strain. These mice carry nine pairs of metacentric Robertsonian translocation chromosomes. As the chromosomes of the wild-type mouse are all acrocentric, metaphase spreads of the WMP2 cells seam to be highly suited for physical gene mapping. Here we studied the WMP2 line using spectral karyotyping (SKY) combined with new established mouse specific multicolor banding (mcb) probes for the chromosomes X, 3, 4, 6 and 18. SKY revealed that the WMP2 cell line developed further four derivative chromosomes. After application of mcb five previously unrecognizable intrachromosomal rearrangements with 9 breakpoints were detected for the studied chromosomes.
Karst
Constanze
C
Institute of Human Genetics and Anthropology, D-07743 Jena, Germany.
Trifonov
Vladimir
V
Romanenko
Svetlana A
SA
Claussen
Uwe
U
Mrasek
Kristin
K
Michel
Susanne
S
Avner
Philip
P
Liehr
Thomas
T
D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D033481:Chromosomes, Mammalian / Q000235:genetics; D003116:Color; D005260:Female; D008297:Male; D051379:Mice; D015341:Nucleic Acid Probes / Q000032:analysis* / Q000235:genetics*; D032681:Spectral Karyotyping
D015341:Nucleic Acid Probes