title: Rapid identification of bacteria by real-time amplification and sequencing of the 16S rRNA gene.
abstract: To allow rapid identification of bacteria in pure cultures and blood culture bottles, an assay was developed which is based on real-time amplification and sequencing of bacterial 16 S rRNA genes. In principle, this assay allows identification of bacteria from pure cultures within 6.5 h, and from blood cultures within approximately 7 h.
authors.affiliation: Department of Medical Microbiology, University Hospital Maastricht, Maastricht Infection Center, PO Box 5800, 6202 AZ Maastricht, the Netherlands.
authors.forename: Inge
authors.initials: I
authors.lastname: Vliegen
authors.forename: Jan A
authors.initials: JA
authors.lastname: Jacobs
authors.forename: Erik
authors.initials: E
authors.lastname: Beuken
authors.forename: Cathrien A
authors.initials: CA
authors.lastname: Bruggeman
authors.forename: Cornelis
authors.initials: C
authors.lastname: Vink
mesh_terms: D001420:Bacteria, Aerobic / Q000235:genetics* / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D005456:Fluorescent Dyes / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005456:Fluorescent Dyes; D012336:RNA, Ribosomal, 16S