title: Online LC-MS-MS process monitoring for optimization of biological treatment of wastewater containing azo dye concentrates.
abstract: A biological high-performance treatment process comprising two 40-L reactor compartments has been developed for purification and decoloration of concentrated textile wastewater containing up to 15 g L(-1) reactive dyestuff. The decoloration rate of 95% meets the requirements of German legislation for textile wastewater treatment. Successful process development and optimization was achieved by use of high-performance liquid chromatography, with diode-array and electrospray tandem mass spectrometric (LC-ESI-MS-MS) detection, coupled with inline microfiltration membrane-sampling devices, applied online to bioreactors as a process analytical tool for the first time. The optimum process performance was found by correlation of dye and decoloration product-specific concentrations with summary properties such as redox potential and dissolved oxygen content. Details of the degradation and decoloration mechanism for the azo dye reactive black 5 was revealed by using mass spectrometry for structure elucidation.
authors.affiliation: Faculty 09, Institute of Chemical Process Engineering and Plant Design, University of Applied Sciences Cologne, Betzdorfer Strabe 2, 50679 Köln, Germany. astrid.rehorek@fh-koeln.de
authors.forename: Astrid
authors.initials: A
authors.lastname: Rehorek
authors.forename: Alexander
authors.initials: A
authors.lastname: Plum
mesh_terms: D001391:Azo Compounds / Q000032:analysis*; D001673:Biodegradation, Environmental; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D004396:Coloring Agents / Q000032:analysis*; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D014874:Water Pollutants, Chemical