title: Changes in microRNA expression profiles in HIV-1-transfected human cells.
abstract: MicroRNAs (miRNAs) are small RNAs of 18-25 nucleotides (nt) in length that play important roles in regulating a variety of biological processes. Recent studies suggest that cellular miRNAs may serve to control the replication of viruses in cells. If such is the case, viruses might be expected to evolve the ability to modulate the expression of cellular miRNAs. To ask if expression of HIV-1 genes changes the miRNA profiles in human cells, we employed a high throughput microarray method, termed the RNA-primed Array-based Klenow Enzyme (RAKE) assay. Here, we describe the optimization of this assay to quantify the expression of miRNAs in HIV-1 transfected human cells. We report distinct differences in miRNA profiles in mock-transfected HeLa cells versus HeLa cells transfected with an infectious HIV-1 molecular clone, pNL4-3.
authors.affiliation: Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health Bethesda, Maryland 20892-0460, USA. yeungm@niaid.nih.gov
authors.forename: Man Lung
authors.initials: ML
authors.lastname: Yeung
authors.forename: Yamina
authors.initials: Y
authors.lastname: Bennasser
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Myers
authors.forename: Guojian
authors.initials: G
authors.lastname: Jiang
authors.forename: Monsef
authors.initials: M
authors.lastname: Benkirane
authors.forename: Kuan-Teh
authors.initials: KT
authors.lastname: Jeang
mesh_terms: D020869:Gene Expression Profiling; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D016133:Polymerase Chain Reaction; D014162:Transfection
chemical_list: D035683:MicroRNAs