title: Chiral high-performance liquid chromatographic separation of the enantiomers of tetrahydropalmatine and tetrahydroberberine, isolated from Corydalis yanhusuo.
abstract: HPLC methods have been developed for chiral resolution of the enantiomers of dl-tetrahydropalmatine (THP) and dl-tetrahydroberberine (THB), two active constituents of Corydalis yanhusuo W.T. Wang. On the analytical scale, good baseline separation of the enantiomers was achieved using cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in both normal-phase and polar organic modes. Validation of the analytical methods, including linearity, limits of detection, recovery, and precision, and semipreparative resolution of dl-THP and dl-THB, were achieved with methanol as mobile phase, without any basic additives, in polar organic mode using cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phases. On the semipreparative scale, small quantities of the individual enantiomers of THP and THB were isolated for study of the chiroptical properties of the individual enantiomers.
authors.affiliation: Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, The Graduate School of Chinese Academy of Sciences, Lanzhou, 730000, PR China.
authors.forename: Zong-De
authors.initials: ZD
authors.lastname: Zhai
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Shi
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Wu
authors.forename: Xing-Ping
authors.initials: XP
authors.lastname: Luo
mesh_terms: D001599:Berberine / Q000031:analogs & derivatives* / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031311:Corydalis / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D013237:Stereoisomerism
chemical_list: D001600:Berberine Alkaloids; D001599:Berberine; C014215:tetrahydropalmatine; C004645:canadine