Detection of electrophysiological indicators of neurotoxicity in human and rat brain slices by a three-dimensional microelectrode array.
Electrophysiological techniques for the assessment of in vitro neurotoxicology have several advantages over other currently-used methods (for example, morphological techniques), including the ability to detect damage at a very early stage. Novel recording techniques based on microelectrode arrays are available, and could improve recording power. In this study, we investigated how a three-dimensional microelectrode array detects the electrophysiological endpoints of neurotoxicity. We conclude that electrophysiology sensitively reveals neurotoxic actions, and that three-dimensional microelectrode arrays could be proposed for use in neurotoxicology as recording tools that allow easy and sensitive multisite recording, from both rodent and human brain tissue.
Köhling
Rüdiger
R
Institute for Physiology, Westfalische Wilhelms-University, Münster, Germany.
Melani
Raffaella
R
Koch
Uwe
U
Speckmann
Erwin-Josef
EJ
Koudelka-Hep
Milena
M
Thiébaud
Pierre
P
Balestrino
Maurizio
M
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000503:physiopathology*; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D005071:Evoked Potentials / Q000187:drug effects; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity*; D006801:Humans; D007608:Kainic Acid / Q000633:toxicity*; D008297:Male; D008839:Microelectrodes; D019579:Neocortex / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D014298:Trimethyltin Compounds / Q000633:toxicity*
D018690:Excitatory Amino Acid Agonists; D014298:Trimethyltin Compounds; C046488:trimethyltin; D007608:Kainic Acid