title: Monitoring cyclodextrin-polyviologen pseudopolyrotaxanes with the Bradford assay.
abstract: Self-assembled multivalent pseudopolyrotaxanes, composed of lactoside-bearing cyclodextrin (CD) rings threaded on linear polyviologen polymers, have been introduced recently as flexible and dynamic neoglycoconjugates. In the course of this research, it was found that polyviologens are responsive to the Bradford assay, which is traditionally highly selective for proteins. The response of the pseudopolyrotaxanes to the Bradford assay was dependant on, and thus indicative of, the degree of threading of the CD rings onto the polyelectrolyte. The assay was then used to report on the threading and dethreading of native and lactoside-bearing alpha-CD rings onto and off of polyviologen chains, a phenomenon which demonstrates the utility of biochemical assays to address problems unique to supramolecular chemistry.
authors.affiliation: California NanoSystems Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, 405 Hilgard Avenue, Los Angeles, CA 90095-1569, USA.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Belitsky
authors.forename: Alshakim
authors.initials: A
authors.lastname: Nelson
authors.forename: J Fraser
authors.initials: JF
authors.lastname: Stoddart
mesh_terms: D003505:Cyclodextrins / Q000032:analysis* / Q000737:chemistry; D006027:Glycosides; D046911:Macromolecular Substances / Q000032:analysis / Q000737:chemistry; D008722:Methods; D020442:Poloxamer / Q000032:analysis*; D011108:Polymers / Q000032:analysis* / Q000737:chemistry; D043862:Rotaxanes / Q000032:analysis*; D014755:Viologens / Q000032:analysis* / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D006027:Glycosides; D046911:Macromolecular Substances; D011108:Polymers; D043862:Rotaxanes; D014755:Viologens; C008245:lactosides; C024627:polymeric viologens; C475674:pseudopolyrotaxane; D020442:Poloxamer