title: Hemoperfusion with an immobilized polymyxin B column reduces the blood level of neutrophil elastase.
abstract: BACKGROUND
  We investigated whether direct hemoperfusion with an immobilized polymyxin B column (DHP with PMX) could reduce the blood level of neutrophil elastase.
  
  
  METHODS
  20 sepsis patients were enrolled in the study. DHP with PMX was performed twice within a 24-hour period. Neutrophil elastase was measured 7 times.
  
  
  RESULTS
  Neutrophil elastase was 468 +/- 75.1 microg/l, while it was 1,531 +/- 201.7 microg/l immediately after the first session, declined to 351 +/- 73.9 microg/l before the second session of DHP with PMX, and increased again to 599.3 +/- 112.7 microg/l immediately after the second session, 328 +/- 73.7 microg/l at 24 h, 264 +/- 39.3 microg/l at 48 h, and 230 +/- 36.1 microg/l at 72 h after DHP with PMX. The levels from 48 h onwards were significantly lower compared with that before treatment.
  
  
  CONCLUSIONS
  DHP with PMX has an overall effect that reduces circulating neutrophil elastase levels.
authors.affiliation: Department of Emergency and Critical Care Medicine, Nihon University School of Medicine, Tokyo, Japan. hkushi@med.nihon-u.ac.jp
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Kushi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Miki
authors.forename: Jun
authors.initials: J
authors.lastname: Nakahara
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Okamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saito
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Tanjoh
mesh_terms: D000327:Adsorption; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006464:Hemoperfusion / Q000379:methods*; D006801:Humans; D019272:Leukocyte Elastase / Q000097:blood* / Q000737:chemistry*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D011112:Polymyxin B / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D008567:Membranes, Artificial; D019272:Leukocyte Elastase; D011112:Polymyxin B