Rapid identification of bacteria by real-time amplification and sequencing of the 16S rRNA gene.
To allow rapid identification of bacteria in pure cultures and blood culture bottles, an assay was developed which is based on real-time amplification and sequencing of bacterial 16 S rRNA genes. In principle, this assay allows identification of bacteria from pure cultures within 6.5 h, and from blood cultures within approximately 7 h.
Vliegen
Inge
I
Department of Medical Microbiology, University Hospital Maastricht, Maastricht Infection Center, PO Box 5800, 6202 AZ Maastricht, the Netherlands.
Jacobs
Jan A
JA
Beuken
Erik
E
Bruggeman
Cathrien A
CA
Vink
Cornelis
C
D001420:Bacteria, Aerobic / Q000235:genetics* / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D005456:Fluorescent Dyes / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D005456:Fluorescent Dyes; D012336:RNA, Ribosomal, 16S