Evaluation of liver damage after application of TVE in the rat model.
BACKGROUND
The aim of this study was to investigate the effects of total vascular exclusion (TVE) on the liver during the early period of reperfusion.


METHODS
Forty Wistar-Albino rats were divided into four groups. Portal pedicle clamping (groups 1 and 2) or TVE (groups 3 and 4) were applied for 10 minutes. Samples were collected at the time of clamp release (groups 1 and 3) and at 30 minutes of reperfusion (groups 2 and 4). We examined oxidative injury to and histopathology of the liver.


RESULTS
Oxidative stress was more prominent with TVE application. Significant alterations were shown in hepatic superoxide dismutase, catalase, glutathione, and glutathione S-transferase levels. The levels of malondialdehyde and myeloperoxidase were not altered significantly.


CONCLUSIONS
Inflow-outflow occlusion of the liver causes more oxidative stress compared with inflow occlusion.
Darilmaz
G
G
First Department of Surgery, Ankara Numune Training and Research Hospital, Ankara, Turkey.
Topaloglu
S
S
Topaloglu
E
E
Ozel
H
H
Saygun
O
O
Avsar
F M
FM
Sokmensuer
C
C
Ucar
G
G
Sahin
M
M
Hengirmen
S
S
D000818:Animals; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology*; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008102:Liver Circulation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology*; D013482:Superoxide Dismutase / Q000378:metabolism
D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione