[The inhibitory effects of dumb-bell decoy oligodeoxynucleotides targeting NF-kappaB on the growth of multiple myeloma cells and IL-6 expression].
OBJECTIVE
To investigate the inhibitory effects of dumb-bell decoy oligodeoxynucleotides(ODNs) targeting NF-kappaB on the transcriptional activity of NF-kappaB binding to its cis-elements, growth of multiple myeloma cells 8266 and the expression of IL-6 in 8266 cells.


METHODS
The inhibitory effects of dumbbell decoy ODNs targeting NF-kappaB on the transcriptional activity of NF-kappaB was detected by electrophoretic mobility shift assay(EMSA). 8266 cells were randomly divided into 8266 cells of passage (control), dumb-bell decoy ODNs treatment, and cationic liposome treatment groups. Dumb-bell decoy ODNs of 2, 4 and 8 mg/L were cultured respectively into 8266 cells via cationic liposome vector. Then, the transfected 8266 cells and their supernatant were collected at 8, 12 and 18 h. IL-6 expression was measured by ELISA. Effect of decoy ODNs on the growth of 8266 cells stimulated with IL-6 was measured by MTT Colorimetry assay.


RESULTS
Dumbbell decoy ODNs effectively inhibited the binding of NF-kappaB to its cis-elements in vitro. Different doses of decoy ODNs (2, 4 and 8 mg/L) had different inhibitory effects on IL-6 expression in 8266 cells. After decoy ODNs had been transfected into 8266 cells stimulated by IL-6 via cationic liposome vector, both the growth of 8266 cells and activity of IL-6 were inhibited.


CONCLUSIONS
Dumb-bell decoy ODNs can effectively inhibit the growth of 8266 cells and decrease the expression of IL-6 in myeloma cells through the inhibiting NF-kappaB activity.
Jiang
Hong-li
HL
Hemodialysis Center, The First Clinical Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
Xue
Wu-jun
WJ
Liu
Hua
H
Chen
Wei
W
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D024202:Electrophoretic Mobility Shift Assay; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D009101:Multiple Myeloma / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*
D015850:Interleukin-6; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides