Mammalian mitochondrial nitric oxide synthase: characterization of a novel candidate.
Recently a novel family of putative nitric oxide synthases, with AtNOS1, the plant member implicated in NO production, has been described. Here we present experimental evidence that a mammalian ortholog of AtNOS1 protein functions in the cellular context of mitochondria. The expression data suggest that a candidate for mammalian mitochondrial nitric oxide synthase contributes to multiple physiological processes during embryogenesis, which may include roles in liver haematopoesis and bone development.
Zemojtel
Tomasz
T
Department of Computational Molecular Biology, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany. zemojtel@molgen.mpg.de
Kolanczyk
Mateusz
M
Kossler
Nadine
N
Stricker
Sigmar
S
Lurz
Rudi
R
Mikula
Ivan
I
Duchniewicz
Marlena
M
Schuelke
Markus
M
Ghafourifar
Pedram
P
Martasek
Pavel
P
Vingron
Martin
M
Mundlos
Stefan
S
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004622:Embryo, Mammalian / Q000033:anatomy & histology; D006801:Humans; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism*; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014018:Tissue Distribution
D007527:Isoenzymes; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D019001:Nitric Oxide Synthase