title: [Construction of tumor cell-specific expression vector modulated by human telomerase reserse transcription gene core promoter].
abstract: OBJECTIVE
  To construct the tumor cell-specific expression vector modulated by human telomerase reserse transcription gene (hTERT) core promoter.
  
  
  METHODS
  The hTERT gene core promoter fragment was amplified by PCR using the total genomic DNA from SKOV3 cells as template. The amplified gene fragment was subsequently cloned into pIRES2-EGFP vector. Then the expression vector modulated by hTERT core gene promoter was transfected into tumor cell lines, HO-8910, Hela and normal ECV304 cells through lipofectamine, respectively. The activity of reporter gene GFP was detected 48 h after transfection.
  
  
  RESULTS
  There was significant transcriptional activity in 3 kinds of transfected tumor cells, while no transcriptional activity was detected in transfected normal cells.
  
  
  CONCLUSIONS
  The hTERT core gene promoter has the tumor specificity, suggesting that the specific expression vector modulated by hTERT core gene promoter may be a novel and promising approach to the tumor treatment.
authors.affiliation: Environment and Genes Related to Diseases Key Laboratory of Education Ministry, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. ylhmqq@yahoo.com.cn
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Li
authors.forename: Tian-bao
authors.initials: TB
authors.lastname: Song
authors.forename: Yue-cheng
authors.initials: YC
authors.lastname: Yu
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Huang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D014162:Transfection
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C509186:TERT protein, human; D019098:Telomerase