title: Time course of oxidative stress status in the postprandial and postabsorptive states in type 1 diabetes mellitus: relationship to glucose and lipid changes.
abstract: OBJECTIVE
  The aim of this study was to compare oxidative stress status (OSS) with blood glucose and lipid changes during the fasting, postprandial and postabsorptive phases in type 1 diabetes mellitus.
  
  
  METHODS
  Twenty-three patients on intensive insulin treatment received a standard fat-rich breakfast and lunch. OSS was monitored at fasting (F), just after the post-breakfast glycemia peak (BP) (identified by continuous subcutaneous glucose monitoring), 3.5-h post-breakfast (B3.5), just after the post-lunch peak (LP), just after the post-lunch dale (LD) and 5 hours after lunch (L5).
  
  
  RESULTS
  Whereas whole blood glutathione and plasma protein thiols increased in the postprandial period (from 6.52 +/- 1.20 (F) to 7.08 +/- 1.45 micromol/g Hb (BP), p = 0.005), ascorbate decreased gradually from 44 +/- 17 (F) to 39 +/- 19 micromol/L (LD), p = 0.015. Retinol and alpha-tocopherol also decreased from 27.1 +/- 7.0 (F) to 25.3 +/- 5.2 micromol/L (BP), p = 0.005. Uric acid decreased later, from 213 +/- 77 (BP) to 204 +/- 68 micromol/L (B3.5), p = 0.01, but then increased in LP (231 +/- 70 micromol/L) and LD to values higher than F (215 +/- 64, micromol/L, p = 0.01). Malondialdehyde increased gradually from 1.02 +/- 0.36 (F) to a maximum of 1.14 +/- 0.40 micromol/L (LP). In the postabsorptive phase (L5) all parameters except for thiols reverted to fasting concentrations.
  
  
  CONCLUSIONS
  In type 1 diabetes lipid peroxidation increases during the postprandial phase in parallel to glucose and triglyceride changes. Blood antioxidants, however, followed diverse patterns of change.
authors.affiliation: University of Antwerp campus Drie Eiken, Metabolic Research Unit T4.37, Universiteitsplein 1, B-2610 Wilrijk-Antwerp, Belgium. begona.manuelykeenoy@ua.ac.be
authors.forename: Begoña
authors.initials: B
authors.lastname: Manuel-y-Keenoy
authors.forename: Ann
authors.initials: A
authors.lastname: Van Campenhout
authors.forename: Petra
authors.initials: P
authors.lastname: Aerts
authors.forename: Jan
authors.initials: J
authors.lastname: Vertommen
authors.forename: Pascale
authors.initials: P
authors.lastname: Abrams
authors.forename: Luc F
authors.initials: LF
authors.lastname: Van Gaal
authors.forename: Carolien
authors.initials: C
authors.lastname: Van Gils
authors.forename: Ivo H
authors.initials: IH
authors.lastname: De Leeuw
mesh_terms: D000042:Absorption; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D019540:Area Under Curve; D001786:Blood Glucose / Q000032:analysis*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005215:Fasting / Q000097:blood; D005260:Female; D005502:Food; D005978:Glutathione / Q000097:blood; D006801:Humans; D007328:Insulin / Q000378:metabolism; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D019518:Postprandial Period; D014280:Triglycerides / Q000097:blood
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D014280:Triglycerides; D005978:Glutathione