title: Mitochondrial DNA deletions serve as biomarkers of aging in the skin, but are typically absent in nonmelanoma skin cancers.
abstract: The potential role of mitochondrial DNA (mtDNA) deletions in nonmelanoma skin cancer (NMSC) and in cutaneous photoaging was explored using a genetic approach. Tumors and photodamaged tumor-free "margin" skin were obtained from NMSC patients undergoing excision and the mtDNA from these specimens was screened for the presence of deletions using long extension PCR. mtDNA deletions were abundant in margin tissue specimens from older patients and their number correlated with the patient age. There was a statistically significant difference between the number of mtDNA deletions in tumors and margins. Fewer deletions were detected in the tumors than the margins and the tumors often had no deletions, implying a potential selection for full-length mtDNA or perhaps a protective role for mtDNA deletions in the process of tumorigenesis. The observed mtDNA deletions from skin were often unreported (19 of 21 deletions), but typically shared structural features with mtDNA deletions reported in other tissues. Some mtDNA deletions were detected from the skin of multiple individuals, including 3,715 and 6,278-base pair (bp) deletions, whose frequencies approached that of the previously well-characterized 4977-bp "common" deletion. These data support the use of mtDNA mutations as biomarkers of photoaging in the skin.
authors.affiliation: VA Tennessee Valley Healthcare System, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2600, USA.
authors.forename: Alex
authors.initials: A
authors.lastname: Eshaghian
authors.forename: Ruth A
authors.initials: RA
authors.lastname: Vleugels
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Canter
authors.forename: Michel A
authors.initials: MA
authors.lastname: McDonald
authors.forename: Thomas
authors.initials: T
authors.lastname: Stasko
authors.forename: James E
authors.initials: JE
authors.lastname: Sligh
mesh_terms: D004272:DNA, Mitochondrial / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D017384:Sequence Deletion; D015595:Skin Aging / Q000235:genetics*; D012878:Skin Neoplasms / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers