Termination-free prokaryotic protein translation by using anticodon-adjusted E. coli tRNASer as unified suppressors of the UAA/UGA/UAG stop codons. Read-through ribosome display of full-length DHFR with translated UTR as a buried spacer arm.
Ogawa
Atsushi
A
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Sando
Shinsuke
S
Aoyama
Yasuhiro
Y
D000926:Anticodon; D018388:Codon, Terminator; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010443:Peptide Chain Termination, Translational; D014176:Protein Biosynthesis; D012362:RNA, Transfer, Ser / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D020506:Untranslated Regions
D000926:Anticodon; D018388:Codon, Terminator; D046911:Macromolecular Substances; D012362:RNA, Transfer, Ser; D020506:Untranslated Regions; D013762:Tetrahydrofolate Dehydrogenase