title: Protective effects of melatonin and indole-3-propionic acid against lipid peroxidation, caused by potassium bromate in the rat kidney.
abstract: Potassium bromate (KBrO(3)) is classified as a carcinogenic agent. KBrO(3) induces tumors and pro-oxidative effects in kidneys. Melatonin is a well known antioxidant and free radical scavenger. Indole-3-propionic acid (IPA), an indole substance, also reveals antioxidative properties. Recently, some antioxidative effects of propylthiouracil (PTU)-an antithyroid drug-have been found. The aim of the study was to compare protective effects of melatonin, IPA, and PTU against lipid peroxidation in the kidneys and blood serum and, additionally, in the livers and the lungs, collected from rats, pretreated with KBrO(3). Male Wistar rats were administered KBrO(3) (110 mg/kg b.w., i.p., on the 10th day of the experiment) and/or melatonin, or IPA (0.0645 mmol/kg b.w., i.p., twice daily, for 10 days), or PTU (0.025% solution in drinking water, for 10 days). The level of lipid peroxidation products-malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA)-was measured spectrophotometrically in thyroid homogenates. KBrO(3), when injected to rats, significantly increased lipid peroxidation in the kidney homogenates and blood serum, but not in the liver and the lung homogenates. Co-treatment with either melatonin or with IPA, but not with PTU, decreased KBrO(3)-induced oxidative damage to lipids in the rat kidneys and serum. In conclusion, melatonin and IPA, which prevent KBrO(3)-induced lipid peroxidation in rat kidneys, may be of great value as protective agents under conditions of exposure to KBrO(3).
authors.affiliation: Department of Endocrinology and Metabolic Diseases, Medical University of Łódź, Polish Mother's Memorial Hospital--Research Institute, 281/289 Rzgowska Street, 93-338 Łódź, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Karbownik
authors.forename: Magdalena
authors.initials: M
authors.lastname: Stasiak
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Zygmunt
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Zasada
authors.forename: Andrzej
authors.initials: A
authors.lastname: Lewiński
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001959:Bromates / Q000008:administration & dosage*; D007211:Indoles / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D011441:Propylthiouracil / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001959:Bromates; D007211:Indoles; C019536:potassium bromate; D011441:Propylthiouracil; C015292:indolepropionic acid; D008550:Melatonin