title: Optimization of growth for the hyperthermophilic archaeon Aeropyrum pernix on a small-batch scale.
abstract: Growth of Aeropyrum pernix, the first reported aerobic neutrophilic hyperthermophilic archaeon, was investigated under different cultivation parameters. Different sources of seawater, pH, and the cultivation methods were tested with the aim to improve the biomass production. A 1-L glass flask fitted with a condenser and air diffuser was used as a bioreactor. The optimum conditions for maximizing A. pernix biomass were obtained when Na2S2O3.5H2O (1 g/L) with added marine broth 2216 at pH 7.0 (20 mmol HEPES buffer/L) was used as a growing medium in a 1-L flask. The biomass production was 0.45 g dry cell mass/L in 40 h under the optimum conditions, which is more than the 0.42 g dry cell mass/L in 60 h previously obtained.
authors.affiliation: Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Slovenia.
authors.forename: Igor
authors.initials: I
authors.lastname: Milek
authors.forename: Blaz
authors.initials: B
authors.lastname: Cigic
authors.forename: Mihaela
authors.initials: M
authors.lastname: Skrt
authors.forename: Gönül
authors.initials: G
authors.lastname: Kaletunç
authors.forename: Natasa Poklar
authors.initials: NP
authors.lastname: Ulrih
mesh_terms: D041522:Aeropyrum / Q000254:growth & development*; D018533:Biomass; D003470:Culture Media; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008828:Microbiological Techniques / Q000295:instrumentation / Q000379:methods; D012623:Seawater; D013696:Temperature
chemical_list: D003470:Culture Media