Bone marrow-derived cells expand memory CD8+ T cells in response to viral infections of the lung and skin.
While naive CD8(+) T cells have been shown to require bone marrow-derived dendritic cells (DC) to initiate immunity, such a requirement for memory CD8(+) T cells has had limited assessment. By generating bone marrow chimeras that express the appropriate antigen-presenting molecules on either radiation-sensitive bone marrow-derived or radiation-resistant non-bone marrow-derived compartments, we showed that both primary and secondary immune responses to influenza virus infection of the lung were initiated in the draining LN. This required cells of bone marrow origin, most likely DC, for optimal expansion within the secondary lymphoid compartment. This was similarly the case with HSV-1 infection of the skin. As Langerhans cells are radioresistant, unlike other DC populations, these studies also demonstrate that the radiosensitive DC responsible for secondary expansion of HSV-specific memory are not Langerhans cells.
Belz
Gabrielle T
GT
Division of Immunology, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia. belz@wehi.edu.au
Wilson
Nicholas S
NS
Smith
Christopher M
CM
Mount
Adele M
AM
Carbone
Francis R
FR
Heath
William R
WR
D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology; D016026:Bone Marrow Transplantation; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D006561:Herpes Simplex / Q000235:genetics / Q000276:immunology*; D007156:Immunologic Memory / Q000235:genetics / Q000276:immunology* / Q000528:radiation effects; D007801:Langerhans Cells / Q000276:immunology; D008171:Lung Diseases / Q000235:genetics / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009975:Orthomyxoviridae / Q000235:genetics / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000235:genetics / Q000276:immunology*; D018139:Simplexvirus / Q000276:immunology*; D018183:Transplantation Chimera; D014916:Whole-Body Irradiation