title: Medicinal potential from in vivo and acclimatized plants of Cleome rosea.
abstract: Methanolic extracts obtained from different organs of Cleome rosea, collected from its natural habitat and from in vitro-propagated plants, were submitted to in vitro biological assays. Inhibition of nitric oxide (NO) production by J774 macrophages and antioxidant effects by protecting the plasmid DNA from the SnCl(2)-induced damage were evaluated. Extracts from the stem of both origins and leaf of natural plants inhibited NO production. The plasmid DNA strand breaks induced by SnCl(2) were reduced by extracts from either leaf or stem of both sources. On the other hand, root extracts did not show any kind of effects on plasmid DNA, and presented significant toxic effects to J774 cells. The results showed that C. rosea presents medicinal potential and that the acclimatization process reduces the plant toxicity both to plasmid DNA and to J774 cells, suggesting the use of biotechnology tools to obtain elite plants as source of botanical material for pharmacological and phytochemical studies.
authors.affiliation: Departamento de Biologia Vegetal/Laboratório de Biotecnologia de Plantas (LABPLAN), Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Brazil. csimoes04@yahoo.com.br
authors.forename: Claudia
authors.initials: C
authors.lastname: Simões
authors.forename: José Carlos P
authors.initials: JC
authors.lastname: De Mattos
authors.forename: Kátia C C
authors.initials: KC
authors.lastname: Sabino
authors.forename: Adriano
authors.initials: A
authors.lastname: Caldeira-de-Araújo
authors.forename: Marsen G P
authors.initials: MG
authors.lastname: Coelho
authors.forename: Norma
authors.initials: N
authors.lastname: Albarello
authors.forename: Solange F L
authors.initials: SF
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D031485:Cleome / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D000432:Methanol / Q000737:chemistry; D051379:Mice; D009569:Nitric Oxide / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry / Q000633:toxicity; D010957:Plasmids; D017971:Tin Compounds / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017971:Tin Compounds; C023599:stannous chloride; D009569:Nitric Oxide; D000432:Methanol