title: Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures.
abstract: The ubiE gene of Geobacillus stearothermophilus V, with its own promoter, was cloned and introduced into Escherichia coli. The cloned gene complemented the ubiE gene deficiency of E. coli AN70. In addition, the expression of this gene in E. coli JM109 resulted in the evolution of volatile selenium compounds when these cells were grown in selenite- or selenate-amended media. These compounds were dimethyl selenide and dimethyl diselenide.
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile.
authors.forename: J W
authors.initials: JW
authors.lastname: Swearingen
authors.forename: D E
authors.initials: DE
authors.lastname: Fuentes
authors.forename: M A
authors.initials: MA
authors.lastname: Araya
authors.forename: M F
authors.initials: MF
authors.lastname: Plishker
authors.forename: C P
authors.initials: CP
authors.lastname: Saavedra
authors.forename: T G
authors.initials: TG
authors.lastname: Chasteen
authors.forename: C C
authors.initials: CC
authors.lastname: Vásquez
mesh_terms: D001406:Bacillaceae / Q000201:enzymology* / Q000235:genetics; D003470:Culture Media; D048168:Escherichia coli K12 / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism*; D016566:Organoselenium Compounds / Q000378:metabolism; D064586:Selenic Acid; D018036:Selenium Compounds / Q000378:metabolism*; D014835:Volatilization
chemical_list: D003470:Culture Media; D016566:Organoselenium Compounds; D018036:Selenium Compounds; C037445:dimethyldiselenide; C008493:methylselenic acid; D008780:Methyltransferases; D064586:Selenic Acid; C013503:dimethylselenide