title: Airway remodeling in allergen-challenged Brown Norway rats: distribution of proteoglycans.
abstract: Proteoglycans (PG) have important effects on the mechanical properties of tissues and the phenotype of various structural cells. Little is known about changes in PG deposition in the airways in animal models of asthma. We studied changes in PG in the airway wall of Brown Norway rats sensitized to ovalbumin (OA) and exposed to repeated OA challenge. Control (Sal) animals were sensitized and challenged with saline. After the 3rd challenge, animals were killed and lungs fixed in formalin. Tissue sections were incubated with antibodies to the small, leucine-rich PG, decorin, and biglycan and collagen type I. Airways were classified according to basement membrane perimeter length (< or =0.99, 1-2.99, and > or =3 mm). Decorin, biglycan, and collagen type I were increased in the airways of OA vs. Sal rats. Remodeling was most prominent in central airways. The distribution of PG differed with respect to the subepithelial vs. airway smooth muscle (ASM) vs. adventitial layer. Whereas biglycan was readily detected within the ASM, decorin and collagen were detected outside the ASM and especially in the adventitial layer. Differences in the distribution of these molecules within the layers of the airway wall may reflect their specific functional roles.
authors.affiliation: Meakins Christie Labs, 3626 St. Urbain Street, Montreal, QC, Canada.
authors.forename: Laura
authors.initials: L
authors.lastname: Pini
authors.forename: Chiara
authors.initials: C
authors.lastname: Torregiani
authors.forename: James G
authors.initials: JG
authors.lastname: Martin
authors.forename: Qutayba
authors.initials: Q
authors.lastname: Hamid
authors.forename: Mara S
authors.initials: MS
authors.lastname: Ludwig
mesh_terms: D000276:Adjuvants, Immunologic; D000403:Airway Resistance / Q000276:immunology*; D000485:Allergens; D000818:Animals; D001886:Bordetella pertussis / Q000276:immunology; D047093:Eosinophil Major Basic Protein / Q000032:analysis; D008297:Male; D010047:Ovalbumin; D011509:Proteoglycans / Q000032:analysis*; D051381:Rats; D011914:Rats, Inbred BN; D012016:Reference Values; D020545:Respiratory Mucosa / Q000737:chemistry / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000485:Allergens; D011509:Proteoglycans; D010047:Ovalbumin; D047093:Eosinophil Major Basic Protein