title: Production of completely flavinylated histamine dehydrogenase, unique covalently bound flavin, and iron-sulfur cluster-containing enzyme of nocardioides simplex in Escherichia coli, and its properties.
abstract: The hmd gene of histamine dehydrogenase from Nocardioides simplex was overexpressed in Escherichia coli, and the resulting enzyme was purified to homogeneity. The purified recombinant enzyme is almost identical with the native enzyme in view of molecular weight and specific activity, and is stoichiometrically assembled with the three cofactors 6-S-cysteinyl FMN, 4Fe-4S cluster, and ADP.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Fujieda
authors.forename: Noriaki
authors.initials: N
authors.lastname: Tsuse
authors.forename: Atsuko
authors.initials: A
authors.lastname: Satoh
authors.forename: Tokuji
authors.initials: T
authors.lastname: Ikeda
authors.forename: Kenji
authors.initials: K
authors.lastname: Kano
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D005486:Flavin Mononucleotide / Q000378:metabolism; D005415:Flavins / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D008970:Molecular Weight; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D013056:Spectrophotometry, Ultraviolet; D013455:Sulfur / Q000378:metabolism*
chemical_list: D005415:Flavins; D000244:Adenosine Diphosphate; D013455:Sulfur; D005486:Flavin Mononucleotide; D007501:Iron; D000587:Oxidoreductases Acting on CH-NH Group Donors; C490533:histamine dehydrogenase, Nocardioides simplex