title: Intact carboxysomes in a cyanobacterial cell visualized by hilbert differential contrast transmission electron microscopy.
abstract: Carboxysomes in rapidly frozen ice-embedded whole cells of the cyanobacterium Synechococcus sp. strain PCC 7942 were visualized by the recently developed Hilbert differential contrast transmission electron microscope. Structural details of carboxysomes were especially clearly visualized in the ruptured cells. The novel electron microscopy exhibited the paracrystalline arrays of molecules of the enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase in the carboxysomes in much better contrast than conventional transmission electron microscopy with ultrathin sections of cells. The carboxysome was surrounded by a 5- to 6-nm-thick monolayer shell which consisted of orderly arrays of globular particles.
authors.affiliation: Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Radostin
authors.initials: R
authors.lastname: Danev
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Nagayama
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nakamoto
mesh_terms: D007053:Ice; D046529:Microscopy, Electron, Transmission; D008858:Microscopy, Phase-Contrast; D015388:Organelles / Q000201:enzymology / Q000648:ultrastructure*; D012273:Ribulose-Bisphosphate Carboxylase / Q000648:ultrastructure*; D046940:Synechococcus / Q000201:enzymology / Q000648:ultrastructure*; D016610:Tissue Embedding
chemical_list: D007053:Ice; D012273:Ribulose-Bisphosphate Carboxylase