Cytogenetic effect of technical glyphosate on cultivated bovine peripheral lymphocytes.
A technical herbicide containing isopropyl amine salt of glyphosate was tested for induction of chromosome aberrations (CA) and sister chromatid exchanges (SCE) in cultured bovine peripheral lymphocytes. Cultures were exposed to a glyphosate formulation at concentrations ranging from 28 to 1120 micromol/l without and with metabolic activation. No clastogenic effect of the herbicide was found. Its genotoxic effect was confirmed in the SCE assay after 24 h of incubation. A statistically significant elevation in SCE induction was observed in each of the donors after application of the product at doses ranging from 56 to 1120 micromol/l. The highest concentrations (560 and 1120 micromol/l) also caused reduction of mitotic and proliferation indices. In the 2 h-assay with metabolic activation a statistically significant frequency of SCE was observed only in cultures treated with the agent at a concentration of 140 micromol/l.
Siviková
Katarína
K
Institute of Genetics, University of Veterinary Medicine, Komenského 73, 041 81 Kosice, Slovak Republic. sivikova@uvm.sk
Dianovskỳ
Ján
J
D000818:Animals; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects; D033481:Chromosomes, Mammalian / Q000187:drug effects; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D008214:Lymphocytes / Q000187:drug effects*; D008940:Mitotic Index; D012854:Sister Chromatid Exchange / Q000187:drug effects; D000097797:Glyphosate
D005998:Glycine; D000097797:Glyphosate; C510942:glyphosate, isopropyl amine salt