Analysis for localization of steroid sulfatase in human tissues.
Human steroid sulfatase (STS) is an enzyme that hydrolyzes several sulfated steroids, such as estrone sulfate, dehydroepiandrosterone sulfate, and cholesterol sulfate, and results in the production of active substances. STS has been demonstrated in human breast cancer tissues and is considered to be involved in intratumoral estrogen production. It is very important to analyze the cellular distribution of STS with accuracy in human tissues in order to obtain a better understanding of the biological significance of STS. Therefore, this chapter describes several morphological approaches used to study the localization of STS, including immunohistochemistry, mRNA in situ hybridization, and laser capture microdissection/reverse transcription-polymerase chain reaction, in human tissues.
Suzuki
Takashi
T
Departmen of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Miki
Yasuhiro
Y
Fukuda
Tsuyoshi
T
Nakata
Taisuke
T
Moriya
Takuya
T
Sasano
Hironobu
H
D001943:Breast Neoplasms / Q000737:chemistry / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D042282:Microdissection / Q000379:methods; D009928:Organ Specificity; D010920:Placenta / Q000737:chemistry / Q000166:cytology / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043266:Steryl-Sulfatase / Q000032:analysis* / Q000235:genetics
D012333:RNA, Messenger; D043266:Steryl-Sulfatase