[p42/44 MAPK mediates synthesis of fractalkine by mesangial cells stimulated by connective tissue growth factor].
OBJECTIVE
To examine whether connective tissue growth factor(CTGF) induces the production of fractalkine(FLK) by glomerular mesangial cells of rats, and explore the mechanism of signal pathway of CTGF actions.


METHODS
The mRNA expression of FLK was analyzed by RT-PCR in cultured mesangial cells stimulated by CTGF. The protein of FLK in the supernatants of cells was determined by ELISA. The chemotactic effect of the supernatants on monocytes was assessed by the in vitro chemotaxis assay. The phosphorylation of p42/44 MAPK was assessed by Western blot.


RESULTS
Treatment of the cells with CTGF enhanced the mRNA expression of FLK and concentration of FLK in the supernatants. Pretreatment of the supernatants of CTGF-treated cells with anti-FLK antibodies partially inhibited the chemotactic effect of the supernatants on monocytes. CTGF increased the p42/44MAPK phosphorylation. Pretreatment of the cells with PD98059 or UO126, inhibitors of phosphorylated p42/44 MAKP, decreased the CTGF-induced expression of phosphorylated p42/44 MAPK and concentration of FLK in supernatants.


CONCLUSIONS
CTGF-induced secretion of FLK by mesangial cells was associated with the phosphorylation of p24/p44 MAPK.
Wu
Sheng-hua
SH
Department of Pediatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Lu
Chao
C
Dong
Ling
L
Zhou
Guo-ping
GP
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D054428:Chemokine CX3CL1 / Q000235:genetics / Q000378:metabolism*; D055513:Connective Tissue Growth Factor / Q000494:pharmacology*; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
D054428:Chemokine CX3CL1; D055513:Connective Tissue Growth Factor; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3