Anucleate cell blue assay: a useful tool for identifying novel type II topoisomerase inhibitors.
About 95,000 compounds were screened by the anucleate cell blue assay. Fifty-one of the hit compounds had various structures and showed inhibitory activity against DNA gyrase and/or topoisomerase IV. Moreover, the compounds exhibited antibacterial activity against a fluoroquinolone- and novobiocin-resistant strain of Staphylococcus aureus. The anucleate cell blue assay is therefore a useful tool for finding novel type II topoisomerase inhibitors.
Oyamada
Yoshihiro
Y
Pharmacology & Microbiology Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Enoki 33-94, Suita, Osaka 564-0053, Japan.
Ito
Hideaki
H
Fujimoto-Nakamura
Mika
M
Tanitame
Akihiko
A
Iwai
Noritaka
N
Nagai
Kazuo
K
Yamagishi
Jun-Ichi
J
Wachi
Masaaki
M
D001419:Bacteria / Q000187:drug effects / Q000201:enzymology*; D001681:Biological Assay / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D024841:Fluoroquinolones; D059005:Topoisomerase II Inhibitors
D004396:Coloring Agents; D004791:Enzyme Inhibitors; D024841:Fluoroquinolones; D059005:Topoisomerase II Inhibitors