title: Histamine scavenging attenuates endotoxin-induced acute lung injury.
abstract: Histamine is an important mediator of early and late inflammatory responses. Here we asked whether scavenging of endogenous histamine by the arthropod-derived histamine binding protein EV131 diminishes acute respiratory distress syndrome (ARDS) induced by inhaled endotoxin. We demonstrate that EV131 (360 microg given intranasally) reduced endotoxin-induced bronchoconstriction and recruitment of neutrophils. Furthermore, EV131 administration diminished TNF-alpha and protein leak in the bronchoalveolar lavage fluid. The data suggest that histamine attenuates endotoxin-induced bronchoconstriction and neutrophil recruitment. Therefore, scavenging of histamine by EV131 may represent a novel therapeutic strategy in ARDS.
authors.affiliation: CNRS Institute Transgenose, IEM, Orleans, France. bryffel@cnrs-orleans.fr
authors.forename: Bernhard
authors.initials: B
authors.lastname: Ryffel
authors.forename: Isabelle
authors.initials: I
authors.lastname: Couillin
authors.forename: Isabelle
authors.initials: I
authors.lastname: Maillet
authors.forename: Bruno
authors.initials: B
authors.lastname: Schnyder
authors.forename: Guido C
authors.initials: GC
authors.lastname: Paesen
authors.forename: Patricia
authors.initials: P
authors.lastname: Nuttall
authors.forename: Wynne
authors.initials: W
authors.lastname: Weston-Davies
mesh_terms: D000403:Airway Resistance / Q000187:drug effects / Q000502:physiology; D000818:Animals; D004731:Endotoxins / Q000633:toxicity*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009195:Peroxidase / Q000378:metabolism; D011968:Receptors, Histamine / Q000627:therapeutic use*; D012128:Respiratory Distress Syndrome / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology
chemical_list: C518565:EV131 protein, Rhipicephalus appendiculatus; D004731:Endotoxins; D011968:Receptors, Histamine; D009195:Peroxidase