Radical scavenging and anti-inflammatory activity of extracts from Opuntia humifusa Raf.
Opuntia humifusa Raf. (O. humifusa Raf.) is a member of the Cactaceae family. To determine the antioxidative and anti-inflammatory effects of this herb, various solvent fractions (methanol, hexane, chloroform, ethyl acetate, butanol, and water) prepared from the leaves of cacti were tested using DPPH (2,2-diphenyl-l-picrylhydrazyl radical) and xanthine oxidase assays, and nitric oxide (NO)-producing macrophage cells. We found that O. humifusa Raf. displayed potent antioxidative and anti-inflammatory activity. Thus, all solvent fractions, except for the water layer, showed potent scavenging effects. The scavenging effect of the ethyl acetate fraction was higher than that of the other fractions, with IC50 values of 3.6 and 48.2 microg mL(-1). According to activity-guided fractionation, one of the active radical scavenging principles in the ethyl acetate fraction was found to be quercetin. In contrast, only two fractions (chloroform and ethyl acetate) significantly suppressed nitric oxide production from the lipopolysaccharide (LPS)-activated RAW264.7 cells. In addition, chloroform and ethyl acetate fractions significantly blocked the expression of inducible nitric oxide synthetase (iNOS) and interleukin-6 (IL-6) from the RAW264.7 cells stimulated by LPS. Moreover, ethyl acetate fractions significantly blocked the expression of IL-1beta from the RAW264.7 cells stimulated by LPS. Therefore, the results suggested that O. humifusa Raf. may modulate radical-induced toxicity via both direct scavenging activity and the inhibition of reactive species generation, and the modulation of the expression of inflammatory cytokines. Finally, O. humifusa Raf. may be useful as a functional food or drug against reactive species-mediated disease.
Cho
Jae Youl
JY
School of Biotechnology and Bioengineering, Kangwon National University, Chuncheon, Republic of Korea.
Park
Seung-Chun
SC
Kim
Tae-Wan
TW
Kim
Kil-Soo
KS
Song
Jae-Chan
JC
Kim
Sang-Keun
SK
Lee
Hui-Min
HM
Sung
Hye-Jin
HJ
Park
Hwa-Jin
HJ
Song
Yong-Beom
YB
Yoo
Eun-Sook
ES
Lee
Choong-Hwan
CH
Rhee
Man-Hee
MH
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D006834:Hydrazines / Q000378:metabolism; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D029747:Opuntia / Q000737:chemistry*; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006834:Hydrazines; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010851:Picrates; D010936:Plant Extracts; D012333:RNA, Messenger; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D052247:Nitric Oxide Synthase Type II; D014969:Xanthine Oxidase