title: Spectral trends in the fluorescence of single bacterial light-harvesting complexes: experiments and modified redfield simulations.
abstract: In this work we present and discuss the single-molecule fluorescence spectra of a variety of species of light-harvesting complexes: LH2 of Rhodopseudomonas acidophila, Rhodobacter sphaeroides, and Rhodospirillum molischianum and LH1 of Rhodobacter sphaeroides. The emission spectrum of these complexes varies as a function of time as was described in earlier work. For each type of complex, we observe a pronounced and well-reproducible characteristic relationship between the fluorescence spectral parameters of the peak wavelength, width, and asymmetry. This dependence for the LH2 complexes can be quantitatively explained on the basis of a disordered exciton model by varying the static disorder and phonon coupling parameters. In addition, a correlation of the pigment site energies has to be assumed to interpret the behavior of the LH1 complex.
authors.affiliation: Department of Biophysics and Physics of Complex Systems, Division of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands. danielis@nat.vu.nl
authors.forename: Danielis
authors.initials: D
authors.lastname: Rutkauskas
authors.forename: Vladimir
authors.initials: V
authors.lastname: Novoderezhkin
authors.forename: Andrew
authors.initials: A
authors.lastname: Gall
authors.forename: John
authors.initials: J
authors.lastname: Olsen
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Cogdell
authors.forename: C Neil
authors.initials: CN
authors.lastname: Hunter
authors.forename: Rienk
authors.initials: R
authors.lastname: van Grondelle
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D001703:Biophysics / Q000379:methods; D003198:Computer Simulation; D004058:Diffusion; D004583:Electrons; D006860:Hydrogen Bonding; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry; D009154:Mutation; D016011:Normal Distribution; D010860:Pigments, Biological / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016992:Rhodobacter / Q000378:metabolism; D012241:Rhodopseudomonas / Q000378:metabolism; D012246:Rhodospirillum / Q000378:metabolism; D013045:Species Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013053:Spectrophotometry; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D010860:Pigments, Biological; D008274:Magnesium