Lethal action of quinolones against a temperature-sensitive dnaB replication mutant of Escherichia coli.
Inhibition of DNA replication in an Escherichia coli dnaB-22 mutant failed to block quinolone-mediated lethality. Inhibition of protein synthesis by chloramphenicol inhibited nalidixic acid lethality and, to a lesser extent, ciprofloxacin lethality in both dnaB-22 and wild-type cells. Thus, major features of quinolone-mediated lethality do not depend on ongoing replication.
Zhao
Xilin
X
Public Health Research Institute, 225 Warren St., Newark, New Jersey 07103, USA.
Malik
Muhammad
M
Chan
Nymph
N
Drlica-Wagner
Alex
A
Wang
Jian-Ying
JY
Li
Xinying
X
Drlica
Karl
K
D000890:Anti-Infective Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002701:Chloramphenicol / Q000494:pharmacology; D004265:DNA Helicases / Q000235:genetics*; D004261:DNA Replication / Q000187:drug effects*; D053488:DnaB Helicases; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D009154:Mutation; D015363:Quinolones / Q000494:pharmacology*; D013696:Temperature
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D015363:Quinolones; D002701:Chloramphenicol; D004265:DNA Helicases; D053488:DnaB Helicases