title: [Alteration of hydrogen sulfide/cystathionine-beta-synthase system in rats with recurrent febrile seizures].
abstract: OBJECTIVE
  To study the alteration of hydrogen sulfide (H(2)S)/ cystathionine-beta-synthase (CBS) system during recurrent febrile seizures (FS) in the hippocampus of developing rats.
  
  
  METHODS
  The rats were randomly divided into control group (n=8) and hyperthermia-treated group (n=22). Which was subdivided into FS group (n=8) and H group(no seizure occurred, n=9) according to whether seizures occurred. The plasma level of H(2)S was detected by the spectrophotometer. The expression levels of CBS gene and protein were examined by in situ hybridization and immunohistochemistry respectively.
  
  
  RESULTS
  The plasma levels of H(2)S were increased significantly in FS group compared with those of control group or H group. The expression levels of CBS gene and protein were enhanced in FS group compared with those of control group or H group.
  
  
  CONCLUSIONS
  The expression levels of H(2)S/ CBS system were up-regulated during recurrent FS.
authors.affiliation: Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: Jiong
authors.initials: J
authors.lastname: Qin
authors.forename: Xing-zhi
authors.initials: XZ
authors.lastname: Chang
authors.forename: Zhi-xian
authors.initials: ZX
authors.lastname: Yang
authors.forename: Jun-bao
authors.initials: JB
authors.lastname: Du
mesh_terms: D000818:Animals; D003541:Cystathionine beta-Synthase / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D006862:Hydrogen Sulfide / Q000097:blood*; D006979:Hyperthermia, Induced; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012008:Recurrence; D003294:Seizures, Febrile / Q000097:blood / Q000235:genetics / Q000503:physiopathology*; D013053:Spectrophotometry; D015854:Up-Regulation
chemical_list: D003541:Cystathionine beta-Synthase; D006862:Hydrogen Sulfide