title: [Antibiotics resistance of meticilline-resistant Staphylococcus aureus: detection of the first glycopeptides low sensibility strains in Tunisia].
abstract: The adaptation of Staphylococcus aureus to the hospital environment led to the acquisition of resistance to all antibiotics available in clinical practice. The aim of this study was to investigate the methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in the F. Bourguiba hospital of Monastir (Tunisia). We determined the antibiotype of all Staphylococcus aureus strains identified. Susceptibility rates to fosfomycin, chloramphenicol, rifampicin and pristinamycin were 7%, 3%, 2% and 0%, respectively. The prevalence of MRSA was 15.5% (96 strains); their susceptible to gentamicin progressively increased. The minimum inhibitory concentration (MICs) of oxacillin, vancomycin and teicoplanin were evaluated for the 96 MRSA strains. We identified two MRSA strains (M4 and M41) showing reduced glycopeptides susceptibility. Further analysis revealed that M4 and M41 harbor the gene encoding the class S and class F proteins specific for the Panton-Valentine Leukocidin (PVL). The mecA gene was detected only in strain M41 which harbors the Staphylococcal Cassette Chromosome (SCCmec) type III. This is the first reported MRSA showing reduced susceptibility to glycopeptides in Tunisia. Regulatory surveillance of susceptibility to antibiotics is needed to reduce the morbidity and the mortality rates as well as societal costs of S. aureus infections.
authors.affiliation: Laboratoire de microbiologie, centre hospitalo-universitaire F.-Bourguiba, Monastir 5000, Tunisie. mastourimaha@yahoo.fr
authors.forename: M
authors.initials: M
authors.lastname: Mastouri
authors.forename: M
authors.initials: M
authors.lastname: Nour
authors.forename: M
authors.initials: M
authors.lastname: Ben Nejma
authors.forename: O
authors.initials: O
authors.lastname: Bouallegue
authors.forename: M
authors.initials: M
authors.lastname: Hammami
authors.forename: M
authors.initials: M
authors.lastname: Khedher
mesh_terms: D000064:Acclimatization; D003428:Cross Infection / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D005839:Gentamicins / Q000627:therapeutic use; D006020:Glycopeptides / Q000032:analysis*; D006801:Humans; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000635:transmission; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D014416:Tunisia
chemical_list: D005839:Gentamicins; D006020:Glycopeptides; D008712:Methicillin