title: [DNA content analysis in thyroid neoplasms: diagnostic and prognostic interest].
abstract: Thyroid nodules are frequent and sometimes they pose a diagnostic and prognostic problem. DNA ploidy study and cell cycle analysis could be of value in the distinction between benign tumors and malignant tumors. Formalin-fixed and paraffin-embedded tissues from 69 patients with different benign and neoplastic lesions were investigated. Nuclear DNA content in thyroid cells was measured after Feulgen staining using SAMBA 200 image analysis system. A diploid DNA stemline was revealed in 75% of histologically proven benign thyroid tumors (15/20) and aneuploidy was found in 57.2% of malignant tumors (28/49). There is a significant correlation between aneuploidy and extra-thyroid extension (p=0.007) and bilateral and/or mediastinal lymph node metastasis (p=0.02). In the majority of benign tumors (19/20), the proliferation index was lower than 3% (< or =3%) however, this index value was higher than 3% (>3%) in more than 83% of malignant tumors (41/49) (p<0.001). The S phase fraction analysis revealed that the threshold of 14% divide the near whole of benign and malignant tumors (p<0.001). Our findings show that in follicular lesions, proliferation index and S phase fraction study appears interesting and helpful in the distinction between benign and malignant tumors, and aneuploidy seems more interesting in prognosis evaluation of these tumors.
authors.affiliation: Laboratoire d'Anatomie et de Cytologie Pathologique, Centre Hospitalo-Universitaire Farhat Hached, Sousse, Tunisie.
authors.forename: N
authors.initials: N
authors.lastname: Missaoui
authors.forename: S
authors.initials: S
authors.lastname: Hmissa
authors.forename: M
authors.initials: M
authors.lastname: Mokni
authors.forename: A
authors.initials: A
authors.lastname: Trabelsi
authors.forename: M
authors.initials: M
authors.lastname: Trimech
authors.forename: I
authors.initials: I
authors.lastname: Lagueb
authors.forename: M T
authors.initials: MT
authors.lastname: Yacoubi
authors.forename: B
authors.initials: B
authors.lastname: Sriha
authors.forename: S
authors.initials: S
authors.lastname: Korbi
mesh_terms: D000782:Aneuploidy; D002455:Cell Division; D002467:Cell Nucleus / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011379:Prognosis; D016196:S Phase; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology
chemical_list: D004247:DNA