Identification of differentially expressed proteins during larval molting of Helicoverpa armigera.
Insect molting involves many molecular processes, such as protein degradation and protein synthesis in the epidermis. Various proteins have been implicated in these processes. The differentially expressed proteins during larval molting of Helicoverpa armigera were investigated using two-dimensional electrophoresis (2-D-PAGE) and matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALTI-TOF-MS). Four larval tissues sampled during molting and feeding were examined. Seventy-seven differentially expressed proteins were identified in these tissues, including 20 proteins from the fifth-molting epidermis (fifth instar molting to sixth instar), 36 proteins from the fifth-molting hemolymph, and 21 from the fifth-molting fat bodies. No obviously different spots were identified from the fifth-molting midgut under these experimental conditions. After application of MALTI-TOF-MS and similarity analysis comparing results to a Drosophila protein database, 30 proteins were identified: 10 proteins from the fifth-molting epidermis, 11 proteins from the hemolymph, and 9 proteins from fat bodies. These proteins were separated into 5 groups according to their probable functions, such as enzymes, regulators, protein hydrolases, receptors, and proteins with unknown functions. These differentially expressed proteins were proposed to be involved in the Helicoverpa molting cascade.
Zhao
Xiao-Fan
XF
School of Life Sciences, Shandong University, Jinan 250100, China. xfzhao@sdu.edu.cn
He
Hong-Juan
HJ
Dong
Du-Juan
DJ
Wang
Jin-Xing
JX
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D019476:Insect Proteins / Q000032:analysis* / Q000378:metabolism; D007814:Larva / Q000737:chemistry / Q000378:metabolism; D007915:Lepidoptera / Q000737:chemistry* / Q000378:metabolism; D018978:Molting; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D019476:Insect Proteins