[The influence of the extract of Ungernia victoris cultured cells and of some metal cations on Escherichia coli transformation with plasmid DNA].
In the system of cation-induced E. coli transformation by the plasmid pBR322 the effects of the extract derived from the biomass of cultured cells of U. victoris on the correlation between yield of transformants, viability of CaCl2 - treated cells and plasmid DNA conformation alterations has been investigated. The data obtained have been compared with effects of some salts of one- and divalent metals on the same parameters. The presence of different mechanisms of the variations of cell population viability and yield of transformants depending on utilization of salts or plant extracts has been shown.
Miriuta
A Iu
AIu
Pererva
T P
TP
Mozhilevskia
L P
LP
Kunakh
V A
VA
D002413:Cations, Divalent / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D008670:Metals / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000166:cytology*; D014169:Transformation, Bacterial / Q000187:drug effects*
D002413:Cations, Divalent; D004269:DNA, Bacterial; D008670:Metals; D010936:Plant Extracts