[The microstructure studies of penile cavernous tissue of erectile dysfunction rat models with chronic renal failure].
OBJECTIVE
To investigate the mechanism of erectile dysfunction (ED) in rat models with chronic renal failure (CRF).


METHODS
Chronic renal failure was induced by adult male Sprague-Dawley rats, which were subjected to an excisional 5/6 nephrectomy. The rats in NCRF group and CRF group were divided into three groups randomly. Injected with apomorphine(APO, 80 microg/kg), penile erections of three groups were observed and noted by the 2nd week, 4th week respectively and 6th week from the 14th day of 5/6 nephrectomy and experimental models of ED with CRF were selected; NOS activity was examined and the microstructures of penile were observed under optical microscope with computer configuration image analysis system in selected rat models.


RESULTS
Compared with the controls, the areas of smooth muscle and NOS activity in the penile cavernous tissue of ED rats with CRF decreased significantly (P < 0.01 or P < 0.05) , and collagen fibers slightly increased, and these alterations had close correlations with the duration of CRF. The


CONCLUSIONS
Penile erection is seriously affected in rats with CRF. The decreases of areas of blood sinus had no obvious changes. the number of smooth muscles and NOS activity might be the most important factors.
Wu
Jian-jun
JJ
Department of Urology, Chengdu Sixth People's Hospital, Chengdu, Sichuan 610051, China. wujj76@hotmail.com
Shao
Ji-chun
JC
Wang
Jiu-yuan
JY
Zhang
Shu-wu
SW
Chang
De-gui
DG
Huang
Xiu-shen
XS
D000818:Animals; D004195:Disease Models, Animal; D007172:Erectile Dysfunction / Q000201:enzymology / Q000473:pathology*; D007676:Kidney Failure, Chronic / Q000150:complications* / Q000473:pathology; D008297:Male; D009130:Muscle, Smooth / Q000000981:diagnostic imaging; D009392:Nephrectomy; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010413:Penis / Q000000981:diagnostic imaging* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014463:Ultrasonography
D019001:Nitric Oxide Synthase