title: Novel PCR-restriction fragment length polymorphism analysis for rapid typing of staphylococcal cassette chromosome mec elements.
abstract: We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV.
authors.affiliation: Graduate School of Life Science and Biotechnology, College of Medicine, Korea University, Seoul.
authors.forename: Jin Ah
authors.initials: JA
authors.lastname: Yang
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Park
authors.forename: Jang Wook
authors.initials: JW
authors.lastname: Sohn
authors.forename: Min Ja
authors.initials: MJ
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial; D006801:Humans; D008712:Methicillin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D045522:Recombinases / Q000235:genetics*; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C489156:CcrC protein, Staphylococcus aureus; D045522:Recombinases; D008712:Methicillin