[Synthesis and DNA binding spectroscopy studies of Cu(II)-Thr-Phen].
A new complex [Cu (Thr)(Phen)H2O] SO4 . HO2 x CH3 OH (Thr = DL-Threonine, Phen = o-Phenanthroline), which has not been published, was synthesized and characterized by elemental analysis, IR spectroscopy, and TG-DTA. The interaction of the complex and sperm DNA was studied by electronic absorption and ethidium bromide (EB) fluorescence spectroscopy. The result indicates that the maximal absorption peaks of this complex are red-shifted and the intensity is weakened; At the same time, it can to some extent quench the emission intensity of EB-DNA system. Therefore, the authors come to a conclusion that the interaction of this complex and sperm DNA is intercalation.
Zhang
Fang
F
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China.
Zhang
Qian-qian
QQ
Zhu
Chen-jian
CJ
Wang
Xiu-lin
XL
D000818:Animals; D001665:Binding Sites; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004996:Ethidium / Q000031:analogs & derivatives / Q000737:chemistry; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D008297:Male; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D010618:Phenanthrolines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013055:Spectrophotometry, Infrared; D013094:Spermatozoa / Q000378:metabolism; D013912:Threonine / Q000737:chemistry*
D007364:Intercalating Agents; D009942:Organometallic Compounds; D010618:Phenanthrolines; C052890:ethidium-DNA complex; D013912:Threonine; D003300:Copper; D004247:DNA; D004996:Ethidium