[The effect on the differentiation and maturation of dendritic cells by lupane acetate of cortex periplocae].
OBJECTIVE
To investigate the effect of lupane acetate of cortex periplocae (CPLA) on the differentiation, maturation and immune activity of human peripheral blood mononuclear cell (PBMC)-derived dendritic cells (DCs) in vitro.


METHODS
PBMC isolated from human peripheral blood was cultured with GM-CSF, IL-4 for 5 d and stimulated with TNF-alpha (as positive control) or CPLA to induce DCs. The morphological characteristics of DC were observed under inverted microscope and transmisson electron microscope. The expressions of CD1a, CD83, CD80 and CD86 were analyzed by flow cytometry. IL-12, IFN-gamma production in the culture supernatant of DCs was detected by ELISA. MTT method was used to determine the proliferation of T cells stimulated by DCs.


RESULTS
After 10-days culture with cytokines and CPLA, PBMC developed into mature DCs with typical morphological characteristics and high expressions of CD1a, CD83, CD80 and CD86 on the cellular surface (P<0.05). CPLA enhanced IL-12 and IFN-gamma production by DCs (P<0.05). CPLA-treated DCs markedly stimulated proliferation of T cells (P<0.05).


CONCLUSIONS
CPLA may induce the differentiation and maturation of DC, up-regulate cytokines production and increase the immune activity of DC.
Zhang
Jing
J
Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China.
Shan
Bao-en
BE
Zhang
Chao
C
Zhao
Rui-nian
RN
Li
Qi-liang
QL
Liu
Jiang-hui
JH
Li
Qiao-min
QM
Li
Run-qing
RQ
D015703:Antigens, CD / Q000378:metabolism; D018949:Antigens, CD1 / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D019684:Magnoliopsida / Q000737:chemistry*; D008562:Membrane Glycoproteins / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D010946:Plants, Medicinal / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*; D000097802:CD83 Antigen; D053978:Pentacyclic Triterpenes
D015703:Antigens, CD; D018949:Antigens, CD1; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D004365:Drugs, Chinese Herbal; D007136:Immunoglobulins; D018664:Interleukin-12; D008562:Membrane Glycoproteins; D014315:Triterpenes; D000097802:CD83 Antigen; C419393:CD1a antigen; C480546:lupane; D053978:Pentacyclic Triterpenes