Spectral trends in the fluorescence of single bacterial light-harvesting complexes: experiments and modified redfield simulations.
In this work we present and discuss the single-molecule fluorescence spectra of a variety of species of light-harvesting complexes: LH2 of Rhodopseudomonas acidophila, Rhodobacter sphaeroides, and Rhodospirillum molischianum and LH1 of Rhodobacter sphaeroides. The emission spectrum of these complexes varies as a function of time as was described in earlier work. For each type of complex, we observe a pronounced and well-reproducible characteristic relationship between the fluorescence spectral parameters of the peak wavelength, width, and asymmetry. This dependence for the LH2 complexes can be quantitatively explained on the basis of a disordered exciton model by varying the static disorder and phonon coupling parameters. In addition, a correlation of the pigment site energies has to be assumed to interpret the behavior of the LH1 complex.
Rutkauskas
Danielis
D
Department of Biophysics and Physics of Complex Systems, Division of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands. danielis@nat.vu.nl
Novoderezhkin
Vladimir
V
Gall
Andrew
A
Olsen
John
J
Cogdell
Richard J
RJ
Hunter
C Neil
CN
van Grondelle
Rienk
R
D001426:Bacterial Proteins / Q000737:chemistry; D001703:Biophysics / Q000379:methods; D003198:Computer Simulation; D004058:Diffusion; D004583:Electrons; D006860:Hydrogen Bonding; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry; D009154:Mutation; D016011:Normal Distribution; D010860:Pigments, Biological / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016992:Rhodobacter / Q000378:metabolism; D012241:Rhodopseudomonas / Q000378:metabolism; D012246:Rhodospirillum / Q000378:metabolism; D013045:Species Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013053:Spectrophotometry; D013997:Time Factors
D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D010860:Pigments, Biological; D008274:Magnesium