title: A novel photon radiation detector system for in vitro biokinetic measurements.
abstract: OBJECTIVE
  This study evaluates a novel photon radiation detector system for in vitro biokinetic measurements.
  
  
  METHODS
  A cell-culture well can be considered to consist of two different compartments: the cells and the medium. By placing the well on a lead-collimated scintillation (NaI(T1)) detector, changes in activity distribution between the compartments will result in changes in count rates owing to the efficiency ratio (ER) between the cells and the medium. The ER depends on differences in detection solid angles for the compartments and differences in attenuation of photons emitted from the compartments. Evaluation of the optimal measuring geometries for the detector system was done by Monte Carlo (MC) simulations. The detector system was tested in two different in vitro situations.
  
  
  RESULTS
  The MC simulations showed that the most optimal detector system was obtained by using a lead-collimated well crystal. Both the MC simulations and the experiments have proven the usability of the system.
  
  
  CONCLUSIONS
  The detector system was demonstrated to be of value for biokinetic studies. The cellular binding of radiolabeled substances can be determined with high precision, and real-time measurements can be performed on a cell culture without harvesting the cells.
authors.affiliation: Department of Radiation Physics, Göteborg University, Göteborg, Sweden. jenny.oddstig@radfys.gu.se
authors.forename: Jenny
authors.initials: J
authors.lastname: Oddstig
authors.forename: Peter
authors.initials: P
authors.lastname: Bernhardt
authors.forename: Lars
authors.initials: L
authors.lastname: Jacobsson
authors.forename: Eva
authors.initials: E
authors.lastname: Forssell-Aronsson
mesh_terms: D003198:Computer Simulation; D007700:Kinetics; D009010:Monte Carlo Method; D017785:Photons / Q000627:therapeutic use; D011829:Radiation Dosage; D011874:Radiometry