The DD-carboxypeptidase activity encoded by pbp4B is not essential for the cell growth of Escherichia coli.
The gene (pbp4B) encoding a putative DD-carboxypeptidase has been deleted in Escherichia coli and it is shown to be not essential for cell division. Disruption of the gene in a genetic background where all putative activities of DD-carboxypeptidases and/or DD-endopeptidases had been eliminated indicates that these activities are not required for cell growth in enterobacteria. The penicillin-binding capacity and a low DD-carboxypeptidase activity of PBP4B are demonstrated.
Vega
Daniel
D
Centro de Biología Molecular Severo Ochoa CSIC-UAM, Campus de Cantoblanco, 28049, Madrid, Spain.
Ayala
Juan A
JA
D004150:Dipeptidases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D017353:Gene Deletion; D046915:Penicillin-Binding Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism
D046915:Penicillin-Binding Proteins; D004150:Dipeptidases; C019989:DD-carboxypeptidase-endopeptidase