title: Effective glycemic control achieved by transplanting non-viral cationic liposome-mediated VEGF-transfected islets in streptozotocin-induced diabetic mice.
abstract: Hypoxic damage is one of the major causes of islet graft failure and VEGF is known to play a crucial role in revascularization. To address the effectiveness of a cationic lipid reagent as a VEGF gene carrier, and the beneficial effect of VEGF-transfected islets on glycemic control, we used effectene lipid reagent in a transfection experiment using mouse islets. Transfection efficiencies were highest for 4 microg/microgL cDNA and 25 microgL effectene and cell viabilities were also satisfactory under this condition, and the overproduction of VEGF mRNA and protein were confirmed from conditioned cells. A minimal number of VEGF-transfected islets (100 IEQ/animal) were transplanted into streptozotocin (STZ)-induced diabetic mice. Hyperglycemia was not controlled in the islet transplantation (IT)-alone group (0/8) (non- diabetic glucose mice number/total recipient mice number) or in the IT-pJDK control vector group (0/8). However, hyperglycemia was completely abrogated in the IT-pJDK-VEGF transduced group (8/8), and viable islets and increased VEGF-transfected grafts vascularization were observed in renal capsules.
authors.affiliation: Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 100-380, Korea.
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Chae
authors.forename: Byung Wan
authors.initials: BW
authors.lastname: Lee
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Oh
authors.forename: You Ran
authors.initials: YR
authors.lastname: Ahn
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Chung
authors.forename: Yong Ki
authors.initials: YK
authors.lastname: Min
authors.forename: Myung Shik
authors.initials: MS
authors.lastname: Lee
authors.forename: Moon Kyu
authors.initials: MK
authors.lastname: Lee
authors.forename: Kwang Won
authors.initials: KW
authors.lastname: Kim
mesh_terms: D000818:Animals; D001835:Body Weight; D002470:Cell Survival; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000378:metabolism; D004195:Disease Models, Animal; D005947:Glucose / Q000494:pharmacology; D005951:Glucose Tolerance Test; D006943:Hyperglycemia / Q000150:complications / Q000378:metabolism* / Q000628:therapy*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000098:blood supply / Q000166:cytology / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D008081:Liposomes / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013311:Streptozocin; D014162:Transfection; D042442:Vascular Endothelial Growth Factors / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*
chemical_list: D007328:Insulin; D008081:Liposomes; D012333:RNA, Messenger; D042442:Vascular Endothelial Growth Factors; D013311:Streptozocin; D005947:Glucose