title: The TIGR Maize Database.
abstract: Maize is a staple crop of the grass family and also an excellent model for plant genetics. Owing to the large size and repetitiveness of its genome, we previously investigated two approaches to accelerate gene discovery and genome analysis in maize: methylation filtration and high C(0)t selection. These techniques allow the construction of gene-enriched genomic libraries by minimizing repeat sequences due to either their methylation status or their copy number, yielding a 7-fold enrichment in genic sequences relative to a random genomic library. Approximately 900,000 gene-enriched reads from maize were generated and clustered into Assembled Zea mays (AZM) sequences. Here we report the current AZM release, which consists of approximately 298 Mb representing 243,807 sequence assemblies and singletons. In order to provide a repository of publicly available maize genomic sequences, we have created the TIGR Maize Database (http://maize.tigr.org). In this resource, we have assembled and annotated the AZMs and used available sequenced markers to anchor AZMs to maize chromosomes. We have constructed a maize repeat database and generated draft sequence assemblies of 287 maize bacterial artificial chromosome (BAC) clone sequences, which we annotated along with 172 additional publicly available BAC clones. All sequences, assemblies and annotations are available at the project website via web interfaces and FTP downloads.
authors.affiliation: The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
authors.forename: Agnes P
authors.initials: AP
authors.lastname: Chan
authors.forename: Geo
authors.initials: G
authors.lastname: Pertea
authors.forename: Foo
authors.initials: F
authors.lastname: Cheung
authors.forename: Dan
authors.initials: D
authors.lastname: Lee
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.forename: Cathy
authors.initials: C
authors.lastname: Whitelaw
authors.forename: Ana C
authors.initials: AC
authors.lastname: Pontaroli
authors.forename: Phillip
authors.initials: P
authors.lastname: SanMiguel
authors.forename: Yinan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Bennetzen
authors.forename: William Brad
authors.initials: WB
authors.lastname: Barbazuk
authors.forename: John
authors.initials: J
authors.lastname: Quackenbush
authors.forename: Pablo D
authors.initials: PD
authors.lastname: Rabinowicz
mesh_terms: D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D030561:Databases, Nucleic Acid; D017343:Genes, Plant; D018745:Genome, Plant; D015698:Genomic Library; D023281:Genomics; D020407:Internet; D012091:Repetitive Sequences, Nucleic Acid; D014584:User-Computer Interface; D003313:Zea mays / Q000235:genetics*