Application of machine learning in SNP discovery.
BACKGROUND
Single nucleotide polymorphisms (SNP) constitute more than 90% of the genetic variation, and hence can account for most trait differences among individuals in a given species. Polymorphism detection software PolyBayes and PolyPhred give high false positive SNP predictions even with stringent parameter values. We developed a machine learning (ML) method to augment PolyBayes to improve its prediction accuracy. ML methods have also been successfully applied to other bioinformatics problems in predicting genes, promoters, transcription factor binding sites and protein structures.


RESULTS
The ML program C4.5 was applied to a set of features in order to build a SNP classifier from training data based on human expert decisions (True/False). The training data were 27,275 candidate SNP generated by sequencing 1973 STS (sequence tag sites) (12 Mb) in both directions from 6 diverse homozygous soybean cultivars and PolyBayes analysis. Test data of 18,390 candidate SNP were generated similarly from 1359 additional STS (8 Mb). SNP from both sets were classified by experts. After training the ML classifier, it agreed with the experts on 97.3% of test data compared with 7.8% agreement between PolyBayes and experts. The PolyBayes positive predictive values (PPV) (i.e., fraction of candidate SNP being real) were 7.8% for all predictions and 16.7% for those with 100% posterior probability of being real. Using ML improved the PPV to 84.8%, a 5- to 10-fold increase. While both ML and PolyBayes produced a similar number of true positives, the ML program generated only 249 false positives as compared to 16,955 for PolyBayes. The complexity of the soybean genome may have contributed to high false SNP predictions by PolyBayes and hence results may differ for other genomes.


CONCLUSIONS
A machine learning (ML) method was developed as a supplementary feature to the polymorphism detection software for improving prediction accuracies. The results from this study indicate that a trained ML classifier can significantly reduce human intervention and in this case achieved a 5-10 fold enhanced productivity. The optimized feature set and ML framework can also be applied to all polymorphism discovery software. ML support software is written in Perl and can be easily integrated into an existing SNP discovery pipeline.
Matukumalli
Lakshmi K
LK
Beltsville Agricultural Research Center, Bovine Functional Genomics Laboratory, US Department of Agriculture, ARS, Beltsville, MD 20705, USA. lmatukum@gmu.edu
Grefenstette
John J
JJ
Hyten
David L
DL
Choi
Ik-Young
IY
Cregan
Perry B
PB
Van Tassell
Curtis P
CP
D000465:Algorithms; D001185:Artificial Intelligence; D001483:Base Sequence; D001665:Binding Sites; D019295:Computational Biology / Q000379:methods*; D020224:Expressed Sequence Tags; D014644:Genetic Variation; D015894:Genome, Human; D018745:Genome, Plant; D006239:Haplotypes; D006720:Homozygote; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011237:Predictive Value of Tests; D016324:Sequence Tagged Sites; D012984:Software; D013025:Glycine max / Q000235:genetics; D014157:Transcription Factors
D014157:Transcription Factors