title: Effects of oral vitamin C supplementation in hemodialysis patients: a proteomic assessment.
abstract: Evidence indicates that oxidative stress is present in dialysis patients, and is associated with vitamin C deficiency. Limited data are available regarding the effects of vitamin C supplementation on oxidative stress and inflammation markers in these patients. Moreover, there are no data available on plasma polypeptide fingerprints by proteome analysis before and after vitamin C supplementation. Therefore, we analyzed plasma samples from a prospective, randomized, open-labeled trial to assess the effects of oral vitamin C supplementation (250 mg three times per week), to define the plasma polypeptide pattern in hemodialysis patients. Our results reveal that more than 30 polypeptides show significant changes in the dialysis patients in comparison to controls with normal renal function, and that several polypeptides are affected/normalized by oral vitamin C supplementation. These results underline the remarkable potential for proteomics to recognize specific peptide profiles in different pathological situations, which might not be detected by classical methods.
authors.affiliation: Medical School of Hannover, Hannover, Germany.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Weissinger
authors.forename: Thao
authors.initials: T
authors.lastname: Nguyen-Khoa
authors.forename: Christine
authors.initials: C
authors.lastname: Fumeron
authors.forename: Claudine
authors.initials: C
authors.lastname: Saltiel
authors.forename: Michael
authors.initials: M
authors.lastname: Walden
authors.forename: Thorsten
authors.initials: T
authors.lastname: Kaiser
authors.forename: Harald
authors.initials: H
authors.lastname: Mischak
authors.forename: Tilman B
authors.initials: TB
authors.lastname: Drüeke
authors.forename: Bernard
authors.initials: B
authors.lastname: Lacour
authors.forename: Ziad A
authors.initials: ZA
authors.lastname: Massy
mesh_terms: D000284:Administration, Oral; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000097:blood; D019587:Dietary Supplements; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D040901:Proteomics; D006435:Renal Dialysis; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000975:Antioxidants; D001205:Ascorbic Acid