[Morpho-functional changes of liver and its regional lymph nodes after exposure to magnetic field of industrial frequency].
The aim of this research was to study the morpho-functional organization of liver and its regional lymph nodes, as well as of other organs of immune system, in animals exposed for 14 days to a magnetic field of industrial frequency (MFIF) with aperiodic oscillations of amplitude of magnetic induction in the range of 5-10 _Ts. The methods of light and electron microscopy were used. Damage to DNA in hepatocyte nuclei was determined using ethidium bromide. Quantitative analysis of the cells in the organs of immune system was performed, blood leukocyte formula was calculated and the activity of oxidative enzymes in blood cells was determined. It was found that MFIF caused micro- and ultrastructural changes in animal liver and its regional lymph nodes, which included the disturbances of hemo- and lymphocirculation, destruction of cellular membranes in some cells, dilation of rough endoplasmic reticulum cisterns, appearance of degenerate mitochondrial forms, glycogen depletion, that collectively indicated the development of tissue hypoxia. The increase in the activity of nuclear endonucleases was detected. Response of organs of immune system to MFIF was manifested by shifts of blast forms/ mature lymphocytes ratio in hepatic regional lymph nodes and by the changes of energy metabolism of blood lymphocytes.
Michurina
S V
SV
Efremov
A V
AV
Shurlygina
A V
AV
Belkin
A D
AD
Vakulin
G M
GM
Verbitskaia
L V
LV
Larionov
P M
PM
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D004247:DNA / Q000032:analysis; D004249:DNA Damage; D022781:Hepatocytes / Q000648:ultrastructure; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000098:blood supply / Q000648:ultrastructure*; D008198:Lymph Nodes / Q000648:ultrastructure*; D008214:Lymphocytes / Q000201:enzymology; D008280:Magnetics; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow; D013154:Spleen / Q000098:blood supply / Q000648:ultrastructure; D013385:Succinate Dehydrogenase / Q000032:analysis; D013950:Thymus Gland / Q000098:blood supply / Q000648:ultrastructure
D004247:DNA; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase