title: [Synthesis and DNA binding spectroscopy studies of Cu(II)-Thr-Phen].
abstract: A new complex [Cu (Thr)(Phen)H2O] SO4 . HO2 x CH3 OH (Thr = DL-Threonine, Phen = o-Phenanthroline), which has not been published, was synthesized and characterized by elemental analysis, IR spectroscopy, and TG-DTA. The interaction of the complex and sperm DNA was studied by electronic absorption and ethidium bromide (EB) fluorescence spectroscopy. The result indicates that the maximal absorption peaks of this complex are red-shifted and the intensity is weakened; At the same time, it can to some extent quench the emission intensity of EB-DNA system. Therefore, the authors come to a conclusion that the interaction of this complex and sperm DNA is intercalation.
authors.affiliation: College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.forename: Qian-qian
authors.initials: QQ
authors.lastname: Zhang
authors.forename: Chen-jian
authors.initials: CJ
authors.lastname: Zhu
authors.forename: Xiu-lin
authors.initials: XL
authors.lastname: Wang
mesh_terms: D000818:Animals; D001665:Binding Sites; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004996:Ethidium / Q000031:analogs & derivatives / Q000737:chemistry; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D008297:Male; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D010618:Phenanthrolines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013055:Spectrophotometry, Infrared; D013094:Spermatozoa / Q000378:metabolism; D013912:Threonine / Q000737:chemistry*
chemical_list: D007364:Intercalating Agents; D009942:Organometallic Compounds; D010618:Phenanthrolines; C052890:ethidium-DNA complex; D013912:Threonine; D003300:Copper; D004247:DNA; D004996:Ethidium