The effects of repeated corticosteroid administration on the neurogenesis in the neonatal rat.
OBJECTIVE
The purpose of this study is to clarify the effects on the brain including neurogenesis pretreated with repeated doses of dexamethasone in the neonatal rat.


METHODS
The 4-day-old Sprague Dawley rats were pretreated with 4 different regimens, namely, single administration of dexamethasone, 2-dose administration, 3-dose administration, and saline administration as a control. Concurrently, bromodeoxyuridine (BrdU), which was incorporated into the dividing cells, was administered. We examined body weight, brain weight, and the number of BrdU-labeled cells in the subventricular zone (SVZ), the subgranular zone (SGZ), and the cortex.


RESULTS
Both the body and brain weight of the rats pretreated with dexamethasone were significantly decreased compared with those given saline. Quantitative analysis of BrdU-labeled cells revealed the significant dose-dependent decreases in the SVZ, the SGZ, and the cortex with the dexamethasone treatment.


CONCLUSIONS
We concluded that the decreases in neurogenesis caused by repeated antenatal corticosteroid therapy might result in the adverse effects on the size of the head at birth.
Kanagawa
Takeshi
T
Department of Obstetrics and Gynecology, Osaka University Faculty of Medicine, Osaka, Japan.
Tomimatsu
Takuji
T
Hayashi
Shusaku
S
Shioji
Mitsunori
M
Fukuda
Hiromitsu
H
Shimoya
Koichiro
K
Murata
Yuji
Y
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development*; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000493:pharmacokinetics; D049109:Cell Proliferation / Q000187:drug effects; D004268:DNA-Binding Proteins; D003907:Dexamethasone / Q000008:administration & dosage* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D007150:Immunohistochemistry; D008938:Mitosis; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley
D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D009687:Nuclear Proteins; D003907:Dexamethasone; D001973:Bromodeoxyuridine