[Effect of Huchang Qingfei concentrated pellets on E-cadherin expression in lung tissue of mice infected with Mycoplasma pneumoniae].
OBJECTIVE
To investigate the effects of Huchang Qingfei concentrated pellets on the expression of E-cadherin (E-cd) in the lung tissue from mice infected with Mycoplasma pneumoniae (MP).


METHODS
A mice model of Mycoplasmal pneumonia (MPP) was developed by repeatedly intranasal infectious route. Transmission electronic microscope (TEM) and immunohistochemistry stain were performed to observe the pathological changes and expression of E-cd in lung tissues.


RESULTS
Under TEM it was found that the cellular membrane was ruptured, mitochondria was denatured, crista was broken in the pulmonary cells of the model group; the all above parameters in Huchang medicated group were improved obviously. The immunohistochemistry test showed that strong positive brown stain of E-cd expression was found in the pulmonary epithelial cell membrane and bronchial periphery in the model group, however, in the medicated group, the E-cd expression level in the cellular membrane was decreased and the expression ratio was dropped significantly as compared with the model controls.


CONCLUSIONS
Huchang Qingfei concentrated pellets can inhibit the overexpression of E-cd in the lung tissue of mice with MP-infection, which may be helpful for prevention and treatment of pulmonary injury caused by MPP.
Wang
Wei-ming
WM
Hei Longjiang Academy of Traditional Chinese Medicine Research Institute, Harbin 150036, China.
Li
Ji-chang
JC
Zhang
Hong-juan
HJ
Yang
Yang
Y
Yang
Gui-ying
GY
D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry; D011019:Pneumonia, Mycoplasma / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D011897:Random Allocation
D015820:Cadherins; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal