Biodegradation during contaminant transport in porous media: 7. Impact of multiple-degrader community dynamics.
The biodegradation and transport of phenanthrene in porous media containing multiple populations of phenanthrene degraders is examined with a series of miscible-displacement experiments. A long-term experiment was conducted with a soil containing an indigenous microbial community comprised of 25 identified phenanthrene-degrading isolates. The rate and magnitude of phenanthrene biodegradation oscillated throughout the six-month experiment. This behavior, at least in part, is attributed to multiple-population dynamics associated with the indigenous community of phenanthrene degraders, the composition of which changed during the experiment. This hypothesis is supported by the results of experiments conducted using sterilized porous media that were inoculated with selected isolates obtained from the indigenous soil community. The results of experiments conducted with sterilized soil inoculated with isolate A exhibited an initial extended period of steady phenanthrene effluent concentrations, followed by a uniform decline. The results of experiments conducted using sterilized sand for single-isolate systems with one of three selected isolates and for systems of two-isolate combinations, indicate the existence of apparent synergistic and antagonistic interactions among the isolates. For example, phenanthrene biodegradation was relatively extensive and occurred without a lag phase for isolate A alone. However, biodegradation was constrained when isolate A and B were combined, indicating an antagonistic interaction. Conversely, whereas extensive lag phases were exhibited by both isolates B and C for the single-isolate experiments, there was minimal lag when isolates B and C were combined, indicating a synergistic interaction.
Wang
Jiann-Ming
JM
Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, Arizona 85721, USA.
Patterson
Brandolyn
B
Bodour
Adria
A
Maier
Raina M
RM
Brusseau
Mark L
ML
D001565:Benzoates / Q000378:metabolism; D001673:Biodegradation, Environmental; D001692:Biological Transport; D003470:Culture Media; D010616:Phenanthrenes / Q000378:metabolism*; D016062:Porosity; D012988:Soil Microbiology; D005464:Fluorobenzenes; D005466:Fluorocarbons
D001565:Benzoates; D003470:Culture Media; D010616:Phenanthrenes; C031181:phenanthrene; C090819:pentafluorobenzoic acid; D005464:Fluorobenzenes; D005466:Fluorocarbons