Acute asthma in children: Relationships among CD14 and CC16 genotypes, plasma levels, and severity.
BACKGROUND
The majority of previous studies investigating asthma genetics have focused on cohorts with stable disease and have not defined mechanisms important during acute asthma. CD14 and CC16 each play a key role in biologically important inflammatory pathways and the gene of each has a functional promoter-region polymorphism.


OBJECTIVE
This study was designed to determine the influence of these polymorphisms on plasma levels of their products and clinical disease during acute asthma. We hypothesized that genotype-related differences in CD14 and CC16 production would be more marked during acute asthma and related to disease severity.


METHODS
We studied 148 children on presentation with acute asthma and again in convalescence. CD14 C-159T and CC16 A38G genotypes were determined, and plasma levels of soluble CD14 (sCD14) and CC16 were measured at both times.


RESULTS
During acute asthma, plasma sCD14 levels were higher for the whole group (p = 0.003), but increases were only in subjects with CD14 -159TT (p = 0.003) and -159CT (p = 0.004), and not in those with -159CC. Plasma CC16 levels were also elevated acutely for the whole group (p = 0.013), but only in those with CC16 38GG (p = 0.043) and 38AG (p = 0.014), and not in those with CC16 38AA. Subjects with CD14 -159CC and CC16 38AA were more likely to have moderate or severe acute asthma.


CONCLUSIONS
Plasma levels of sCD14 and CC16 were higher during acute asthma in the subjects. Those with CD14 -159CC and CC16 38AA had no change in sCD14 and CC16 levels and more severe asthma.
Martin
Andrew C
AC
School of Paediatrics and Child Health, University of Western Australia, Perth, Western Australia, Australia. andrew.martin@health.wa.gov.au
Laing
Ingrid A
IA
Khoo
Siew-Kim
SK
Zhang
Guicheng
G
Rueter
Kristina
K
Teoh
Laurel
L
Taheri
Shahir
S
Hayden
Catherine M
CM
Geelhoed
Gary C
GC
Goldblatt
Jack
J
LeSouëf
Peter N
PN
D000208:Acute Disease; D000293:Adolescent; D001249:Asthma / Q000097:blood* / Q000235:genetics* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000097:blood / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011379:Prognosis; D012189:Retrospective Studies; D012720:Severity of Illness Index; D014598:Uteroglobin / Q000097:blood / Q000235:genetics*
D015415:Biomarkers; D018950:Lipopolysaccharide Receptors; C556389:SCGB1A1 protein, human; D004247:DNA; D014598:Uteroglobin