title: Quasi-confocal fluorescence sectioning with dynamic speckle illumination.
abstract: We present a simple modification to a conventional wide-field fluorescence microscope that provides depth discrimination in thick tissues. The technique consists of illuminating a sample with a sequence of independent speckle patterns and displaying the rms of the resultant sequence of fluorescence images. The advantage of speckle illumination is that it provides diffraction-limited illumination granularity that is highly contrasted even in scattering media. We demonstrate quasi-confocal imaging in a mouse olfactory bulb labeled with green fluorescent protein.
authors.affiliation: Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, Massachusetts 02215, USA.
authors.forename: Cathie
authors.initials: C
authors.lastname: Ventalon
authors.forename: Jerome
authors.initials: J
authors.lastname: Mertz