title: Influence of feeding conditions on clavulanic acid production in fed-batch cultivation with medium containing glycerol.
abstract: First, the effect of different levels of nitrogen source on clavulanic acid (CA) production was evaluated in batch cultivations utilizing complex culture medium containing glycerol and three different levels of soy protein isolate (SPI). Cellular growth, evaluated in terms of the rheological parameter K, was highest with a SPI concentration of 30 g.L(-1) (4.42 g.L(-1) N total). However, the highest production of CA (380 mg.L(-1)) was obtained when an intermediate concentration of 20 g.L(-1) of SPI (2.95 g.L(-1) total N) was used. To address this, the influences of volumetric flow rate (F) and glycerol concentration in the complex feed medium (Cs(F)) in fed-batch cultivations were investigated. The best experimental condition for CA production was F=0.01 L.h(-1) and Cs(F)=120 g.L(-1), and under these conditions maximum CA production was practically twice that obtained in the batch cultivation. A single empirical equation was proposed to relate maximum CA production with F and Cs(F) in fed-batch experiments.
authors.affiliation: Department of Chemical Engineering, Universidade Federal de São Carlos, Cx. Postal 676, CEP 13565-905, São Carlos SP, Brazil.
authors.forename: Juliana C
authors.initials: JC
authors.lastname: Teodoro
authors.forename: Alvaro
authors.initials: A
authors.lastname: Baptista-Neto
authors.forename: Isara L
authors.initials: IL
authors.lastname: Cruz-Hernández
authors.forename: Carlos O
authors.initials: CO
authors.lastname: Hokka
authors.forename: Alberto C
authors.initials: AC
authors.lastname: Badino
mesh_terms: D019149:Bioreactors; D019818:Clavulanic Acid / Q000096:biosynthesis*; D003470:Culture Media; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D030262:Soybean Proteins; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D030262:Soybean Proteins; D019818:Clavulanic Acid; D005990:Glycerol