title: Two dimensional patterning of fluorescent proteins in hydrogels.
abstract: This work describes the successful micropatterning of hybrid systems consisting of hydrogel-dispersed optically active and controllable proteins on solid surfaces without degradation of the photophysical properties of the light-emitting biomolecules. It demonstrates the preservation of the luminescence properties of proteins entrapped into isolated microstructures of poly(acrylamide) gel. This way we can exploit both the structural and function-preserving properties of the hydrogels and the functionality of light-emitting proteins. We believe that this approach can open the way to the realization of nanopatterned optical memories based on photochromic biomolecules.
authors.affiliation: National Nanotechnology Laboratory of Instituto Nazionale di Fisica della Materia-CNR, University of Lecce, I-73100 Lecce, Italy. Francesca.dibenedetto@unile.it
authors.forename: Francesca
authors.initials: F
authors.lastname: Di Benedetto
authors.forename: Adriana
authors.initials: A
authors.lastname: Biasco
authors.forename: Ranieri
authors.initials: R
authors.lastname: Bizzarri
authors.forename: Daniele
authors.initials: D
authors.lastname: Arosio
authors.forename: Fernanda
authors.initials: F
authors.lastname: Ricci
authors.forename: Fabio
authors.initials: F
authors.lastname: Beltram
authors.forename: Roberto
authors.initials: R
authors.lastname: Cingolani
authors.forename: Dario
authors.initials: D
authors.lastname: Pisignano
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D020100:Hydrogels; D066298:In Vitro Techniques; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D013499:Surface Properties
chemical_list: D005456:Fluorescent Dyes; D020100:Hydrogels; C485184:enhanced green fluorescent protein; C485192:green fluorescent protein, Aequorea victoria; D049452:Green Fluorescent Proteins