title: Detection of benzopyrene-deoxyguanosine adducts by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: A method for the detection of BPDE-d guanosine adducts using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is described and illustrated. The results indicate that MALDI is capable of detecting two other DNA benzopyrene adducts, which are trace products formed during the synthesis of BPDE-d guanosine. This MALDI-TOFMS method offers the potential for the detection of DNA adducts in human tissue using very limited sample purification and preparation.
authors.affiliation: Department of Chemistry, Howard University, Washington, DC 20059, USA.
authors.forename: Zhuanhong
authors.initials: Z
authors.lastname: Qiao
authors.forename: Uche
authors.initials: U
authors.lastname: Udeochu
authors.forename: Toiya
authors.initials: T
authors.lastname: Jimerson
authors.forename: Melissa
authors.initials: M
authors.lastname: Fletcher
authors.forename: Oladapo
authors.initials: O
authors.lastname: Bakare
authors.forename: Charles M
authors.initials: CM
authors.lastname: Hosten
mesh_terms: D001580:Benzopyrenes / Q000032:analysis* / Q000737:chemistry*; D018736:DNA Adducts / Q000032:analysis* / Q000737:chemistry*; D003849:Deoxyguanosine / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001580:Benzopyrenes; D018736:DNA Adducts; D003849:Deoxyguanosine