title: Inhibitory effects of Ruta graveolens L. extract on guinea pig liver aldehyde oxidase.
abstract: Ruta graveolens L. is a flavonoid-containing medicinal plant with various biological properties. In the present study, the effects of R. graveolens extract on aldehyde oxidase, a molybdenum hydroxylase, are investigated. Aldehyde oxidase was partially purified from liver homogenates of mature male guinea pigs by heat treatment and ammonium sulphate precipitation. The total extract was obtained by macerating the aerial parts of R. graveolens in MeOH 70% and the effect of this extract on the enzyme activity was assayed using phenanthridine, vanillin and benzaldehyde as substrates. Quercetin and its glycoside form, rutin were isolated, purified and identified from the extract and their inhibitory effects on the enzyme were investigated. R. graveolens extract exhibited a high inhibition on aldehyde oxidase activity (89-96%) at 100 microg/ml which was comparable with 10 microM of menadione, a specific potent inhibitor of aldehyde oxidase. The IC50 values for the inhibitory effect of extract against the oxidation of benzaldehyde, vanillin and phenanthridine were 10.4, 10.1, 43.2 microg/ml, respectively. Both quercetin and rutin at 10 microM caused 70-96% and 27-52% inhibition on the enzyme activity, respectively. Quercetin was more potent inhibitor than rutin, but both flavonols exerted their inhibitory effects mostly in a linear mixed-type.
authors.affiliation: School of Health and Nutrition, Tabriz University of Medical Sciences, Iran.
authors.forename: Saieed
authors.initials: S
authors.lastname: Pirouzpanah
authors.forename: Pirouzpanah
authors.initials: P
authors.lastname: Saieed
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Rashidi
authors.forename: Rashidi Mohammad
authors.initials: RM
authors.lastname: Reza
authors.forename: Abbas
authors.initials: A
authors.lastname: Delazar
authors.forename: Delazar
authors.initials: D
authors.lastname: Abbas
authors.forename: Seyyed-Vali
authors.initials: SV
authors.lastname: Razavieh
authors.forename: Razavieh
authors.initials: R
authors.lastname: Seyyedvali
authors.forename: Aliasghar
authors.initials: A
authors.lastname: Hamidi
authors.forename: Hamidi
authors.initials: H
authors.lastname: Aliasghar
mesh_terms: D042931:Aldehyde Oxidase / Q000037:antagonists & inhibitors*; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000494:pharmacology; D032105:Ruta / Q000737:chemistry*; D012431:Rutin / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D024483:Vitamin K 3 / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D012431:Rutin; D000493:Allopurinol; D024483:Vitamin K 3; D011794:Quercetin; D042931:Aldehyde Oxidase