Cytokine gene expression in peripheral blood mononuclear cells and alloreactivity in hematopoietic cell transplantation with nonmyeloablative conditioning.
Cytokines are thought to play an important role in the pathophysiology of graft-versus-host disease (GVHD). To study the relationship between cytokines and GVHD, we obtained peripheral blood mononuclear cells (MNCs) from 21 patients with hematologic malignancies and their HLA-identical sibling donors before and sequentially after hematopoietic cell transplantation (HCT) with nonmyeloablative conditioning. The MNCs were cultured for 72 hours either alone or in mixed lymphocyte cultures with irradiated MNCs of recipient, donor, or HLA-mismatched third-party origin. The gene expression of interleukin (IL)-2, IL-4, IL-10, IL-18, tumor necrosis factor alpha, and transforming growth factor beta in each culture was then measured by real-time quantitative reverse transcriptase-polymerase chain reaction. The composition of the responder MNCs differed between patients and donors and changed after HCT, with a possible influence on the results. Early after transplantation (day +14), the IL-10 messenger RNA (mRNA) level in response to recipient or donor antigens was higher in patients who did not develop clinically significant acute GVHD when compared with the level in patients who subsequently developed acute GVHD grades II to IV (P = .005 and P = .004, respectively). The IL-10 mRNA level on day +14 was highly correlated with the pretransplantation mRNA level of the recipient MNCs but not with the level of the donor MNCs; this suggests that the IL-10 mRNA detected on day +14 originated from responder cells of recipient origin. A higher IL-10 mRNA level was found in MNCs obtained before transplantation from recipients whose disease progressed or relapsed after the transplantation when compared with the level in patients whose disease did not progress or relapse (P = .03). In conclusion, a high IL-10 gene expression in the recipient MNCs may be related to a reduced incidence of acute GVHD grades II to IV and a reduced graft-versus-tumor effect after HCT with nonmyeloablative conditioning.
Petersen
Søren L
SL
The Lymphocyte Research Laboratory, Department of Hematology, Rigshospitalet, Copenhagen, Denmark. slykkep@rh.dk
Madsen
Hans O
HO
Ryder
Lars P
LP
Svejgaard
Arne
A
Dickmeiss
Ebbe
E
Vindeløv
Lars L
LL
D000328:Adult; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics* / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D006086:Graft vs Host Disease / Q000276:immunology* / Q000473:pathology; D020350:Graft vs Tumor Effect; D019337:Hematologic Neoplasms / Q000097:blood / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D006801:Humans; D007109:Immunity; D016753:Interleukin-10 / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D019172:Transplantation Conditioning / Q000009:adverse effects / Q000379:methods*; D015854:Up-Regulation
D016207:Cytokines; D012333:RNA, Messenger; D016753:Interleukin-10