title: [Cloning and expression of androgen response elements of prostate specific antigen promoter].
abstract: OBJECTIVE
  To provide a possible targeted gene therapy scheme for prostate cancer, and explore the expression efficiency and tissue-specific expression of prostate specific antigen (PSA) promoter.
  
  
  METHODS
  Three plasmids with egfp, pa-EGFP(including ARI, ARII), pba-EGFP (including ARI, ARII, ARIII) and pdeltaba-EGFP (including ARI, ARII and mutated ARIII) were designed, and the expression status was observed by transfecting into HepG2, SMMC-7721, Hela and PC-3.
  
  
  RESULTS
  In prostate cancer cell PC-3, pba-EGFP expressed more GFP than pa-EGFP and pdeltaba-EGFP, which showed that ARIII could notably increase the transcription efficiency of PSA promoter. Further, there was no GFP expression in HepG2, SMMC-7721 and Hela transfected with pa-EGFP, p deltaba-EGFP and pba-EGFP.
  
  
  CONCLUSIONS
  An expression vector based on elements of the PSA gene regulatory sequences has been developed and shown to be tightly regulated in a panel of cells from tissues of various origins. With the tissue-specific functional protein, it should provide a solid platform for clinical studies.
authors.affiliation: Department of Andrology, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hu
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Shang
authors.forename: Jing-ping
authors.initials: JP
authors.lastname: Ge
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Huang
mesh_terms: D000728:Androgens / Q000378:metabolism; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D006801:Humans; D008297:Male; D011401:Promoter Regions, Genetic; D011467:Prostate / Q000378:metabolism; D017430:Prostate-Specific Antigen / Q000096:biosynthesis / Q000235:genetics*; D020218:Response Elements; D014162:Transfection
chemical_list: D000728:Androgens; D017430:Prostate-Specific Antigen