H2A.Z functions to regulate progression through the cell cycle.
Histone H2A variants are highly conserved proteins found ubiquitously in nature and thought to perform specialized functions in the cell. Studies in yeast on the histone H2A variant H2A.Z have shown a role for this protein in transcription as well as chromosome segregation. Our studies have focused on understanding the role of H2A.Z during cell cycle progression. We found that htz1delta cells were delayed in DNA replication and progression through the cell cycle. Furthermore, cells lacking H2A.Z required the S-phase checkpoint pathway for survival. We also found that H2A.Z localized to the promoters of cyclin genes, and cells lacking H2A.Z were delayed in the induction of these cyclin genes. Several different models are proposed to explain these observations.
Dhillon
Namrita
N
Unit on Chromatin and Transcription, National Institute of Child Health and Human Development, National Institutes of Health, Bldg. 18T, Rm. 106, 18 Library Dr., Bethesda, Maryland 20892, USA.
Oki
Masaya
M
Szyjka
Shawn J
SJ
Aparicio
Oscar M
OM
Kamakaka
Rohinton T
RT
D002453:Cell Cycle / Q000502:physiology*; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011401:Promoter Regions, Genetic; D018741:Replication Origin; D016196:S Phase / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
C076558:CLB5 protein, S cerevisiae; C082721:CLN2 protein, S cerevisiae; D019926:Cyclin B; D016213:Cyclins; D006657:Histones; C493530:Htz1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins