title: The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus.
abstract: To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuB(KU80)). Homologous integration reached about 80% for both calcineurin A (calA) and polyketide synthase pksP (alb1) genes in the akuB(KU80) mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuB(KU80) had no influence on pathogenicity in a low-dose murine infection model.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Ciências Farmacêuticas, Universidade de São Paulo, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Márcia Eliana
authors.initials: ME
authors.lastname: da Silva Ferreira
authors.forename: Marcia R V Z
authors.initials: MR
authors.lastname: Kress
authors.forename: Marcela
authors.initials: M
authors.lastname: Savoldi
authors.forename: Maria Helena S
authors.initials: MH
authors.lastname: Goldman
authors.forename: Albert
authors.initials: A
authors.lastname: Härtl
authors.forename: Thorsten
authors.initials: T
authors.lastname: Heinekamp
authors.forename: Axel A
authors.initials: AA
authors.lastname: Brakhage
authors.forename: Gustavo H
authors.initials: GH
authors.lastname: Goldman
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000235:genetics*; D001232:Aspergillus fumigatus / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D019703:Calcineurin / Q000172:deficiency / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005821:Genetic Techniques; D016681:Genome, Fungal; D000072200:Ku Autoantigen; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D011995:Recombination, Genetic / Q000235:genetics*; D014774:Virulence
chemical_list: D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D008741:Methyl Methanesulfonate; D019703:Calcineurin; C000606510:Xrcc6 protein, mouse; D000072200:Ku Autoantigen