title: A novel class of CoA-transferase involved in short-chain fatty acid metabolism in butyrate-producing human colonic bacteria.
abstract: Bacterial butyryl-CoA CoA-transferase activity plays a key role in butyrate formation in the human colon, but the enzyme and corresponding gene responsible for this activity have not previously been identified. A novel CoA-transferase gene is described from the colonic bacterium Roseburia sp. A2-183, with similarity to acetyl-CoA hydrolase as well as 4-hydroxybutyrate CoA-transferase sequences. The gene product, overexpressed in an Escherichia coli lysate, showed activity with butyryl-CoA and to a lesser degree propionyl-CoA in the presence of acetate. Butyrate, propionate, isobutyrate and valerate competed with acetate as the co-substrate. Despite the sequence similarity to 4-hydroxybutyrate CoA-transferases, 4-hydroxybutyrate did not compete with acetate as the co-substrate. Thus the CoA-transferase preferentially uses butyryl-CoA as substrate. Similar genes were identified in other butyrate-producing human gut bacteria from clostridial clusters IV and XIVa, while other candidate CoA-transferases for butyrate formation could not be detected in Roseburia sp. A2-183. This suggests strongly that the newly identified group of CoA-transferases described here plays a key role in butyrate formation in the human colon.
authors.affiliation: Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Cédric
authors.initials: C
authors.lastname: Charrier
authors.affiliation: Scientific Support Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Duncan
authors.affiliation: Scientific Support Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Martin D
authors.initials: MD
authors.lastname: Reid
authors.affiliation: Scientific Support Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Garry J
authors.initials: GJ
authors.lastname: Rucklidge
authors.affiliation: Scientific Support Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Donna
authors.initials: D
authors.lastname: Henderson
authors.affiliation: Scientific Support Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Pauline
authors.initials: P
authors.lastname: Young
authors.affiliation: Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Valerie J
authors.initials: VJ
authors.lastname: Russell
authors.affiliation: Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Rustam I
authors.initials: RI
authors.lastname: Aminov
authors.affiliation: Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Flint
authors.affiliation: Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
authors.forename: Petra
authors.initials: P
authors.lastname: Louis
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism*; D015256:Coenzyme A-Transferases / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D013045:Species Specificity; D013379:Substrate Specificity
chemical_list: D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; D002087:Butyrates; D005227:Fatty Acids; C024343:butyryl-coenzyme A; C009061:propionyl-coenzyme A; D015256:Coenzyme A-Transferases