title: Free radical release and HSP70 expression in two human immune-relevant cell lines after exposure to 1800 MHz radiofrequency radiation.
abstract: The goal of this study was to investigate whether radiofrequency (RF) electromagnetic-field (EMF) exposure at 1800 MHz causes production of free radicals and/or expression of heat-shock proteins (HSP70) in human immune-relevant cell systems. Human Mono Mac 6 and K562 cells were used to examine free radical release after exposure to incubator control, sham, RF EMFs, PMA, LPS, heat (40 degrees C) or co-exposure conditions. Several signals were used: continuous-wave, several typical modulations of the Global System for Mobile Communications (GSM): GSM-non DTX (speaking only), GSM-DTX (hearing only), GSM-Talk (34% speaking and 66% hearing) at specific absorption rates (SARs) of 0.5, 1.0, 1.5 and 2.0 W/kg. Heat and PMA treatment induced a significant increase in superoxide radical anions and in ROS production in the Mono Mac 6 cells when compared to sham and/or incubator conditions. No significant differences in free radical production were detected after RF EMF exposure or in the respective controls, and no additional effects on superoxide radical anion production were detected after co-exposure to RF EMFs+PMA or RF EMFs+LPS. The GSM-DTX signal at 2 W/kg produced a significant difference in free radical production when the data were compared to sham because of the decreasing sham value. This difference disappeared when data were compared to the incubator controls. To determine the involvement of heat-shock proteins as a possible inhibitor of free radical production, we investigated the HSP70 expression level after different RF EMF exposures; no significant effects were detected.
authors.affiliation: University of Rostock, Institute of Cell Biology and Biosystems Technology, Division of Environmental Physiology, D-18059 Rostock, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Lantow
authors.forename: J
authors.initials: J
authors.lastname: Schuderer
authors.forename: C
authors.initials: C
authors.lastname: Hartwig
authors.forename: M
authors.initials: M
authors.lastname: Simkó
mesh_terms: D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D004781:Environmental Exposure; D005609:Free Radicals / Q000276:immunology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000276:immunology / Q000528:radiation effects; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D008872:Microwaves; D009000:Monocytes / Q000276:immunology* / Q000528:radiation effects*; D011829:Radiation Dosage; D011846:Radio Waves
chemical_list: D005609:Free Radicals; D018840:HSP70 Heat-Shock Proteins