Protein sites of attack of N-chlorotaurine in Escherichia coli.
N-Chlorotaurine sodium (NCT) is a promising microbicidal agent for topical treatment of infections. Its targets of attack in Escherichia coli have been investigated by proteomics. Incubation in 1% NCT for 10 and 30 min revealed a change of the charge and a separation of numerous proteins into a series of spots with a different pI. Charge differences could be related to oxidation of cysteine residues to their corresponding sulfonic acids. Heat shock protein 60 appeared, while ribosome-releasing factor, d-ribose periplasmic binding protein, and malonyl-CoA transacylase spots decreased. These results indicate penetration of oxidation capacity into the bacteria and destruction of essential proteins by NCT.
Arnitz
Roland
R
Department of Hygiene, Microbiology and Social Medicine, Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Innsbruck, Austria.
Sarg
Bettina
B
Ott
Helmut W
HW
Neher
Andreas
A
Lindner
Herbert
H
Nagl
Markus
M
D051080:Acyl-Carrier Protein S-Malonyltransferase / Q000378:metabolism; D001426:Bacterial Proteins / Q000187:drug effects; D018834:Chaperonin 60 / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism*; D010084:Oxidation-Reduction; D033902:Periplasmic Binding Proteins / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors*; D040901:Proteomics; D012269:Ribosomal Proteins / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013451:Sulfonic Acids / Q000378:metabolism; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology
D001426:Bacterial Proteins; D018834:Chaperonin 60; D004791:Enzyme Inhibitors; D033902:Periplasmic Binding Proteins; D012269:Ribosomal Proteins; D013451:Sulfonic Acids; C087267:ribosome releasing factor; D013654:Taurine; C043410:N-chlorotaurine; D051080:Acyl-Carrier Protein S-Malonyltransferase; D017346:Protein Serine-Threonine Kinases; D003545:Cysteine