[Research on the polymorphism of heart fatty acid-binding protein gene in Shanxi pig breeds and their crossbred populations using PCR-RFLP].
The genetic variation in 5' - upstream (Hinf I -RFLP)and the second intron (Hinf I *-RFLP, Hae III-RFLP)of heart fatty acid-binding protein(H-FABP)gene were detected with PCR-RFLP in 286 pigs including Mashen, Shanxi white pig and their crossbred populations. The results showed as follows: (1)Mashen presented only DD genotype while other populations varied,and Mashen crossbred populations had only 2 genotypes(DD, Dd) at the Hae III-RFLP site; (2)At the Hinf I -RFLP site of the 5' -upstream region, the crossbred population of Shanxi white pig and Duroc presented only HH genotype while other populations varied. Frequency of h allele in Mashen was 0.9727. (3)At the Hinf I *-RFLP site of the second intron, only Mashen presented 2 genotypes (BB, Bb), and frequency of B allele was 0.9667. (4)At the Hae III-RFLP and Hinf I *-RFLP sites, all populations were in Hardy-weinberg equilibrium.
Yang
Wen-Ping
WP
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China. yangwenping518@163.com
Cao
Guo-Qing
GQ
Xue
Shang-Jun
SJ
Liu
Jian-Hua
JH
Li
Bu-Gao
BG
Zhou
Zhong-Xiao
ZX
D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D015810:Linkage Disequilibrium; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000145:classification / Q000235:genetics*
D050556:Fatty Acid-Binding Proteins