Comparison of hydroxyl radical generation in patients undergoing coronary artery bypass grafting with and without cardiopulmonary bypass.
We measured the hydroxyl radical (.OH) generation in fourteen patients undergoing coronary artery bypass grafting (CABG), of whom seven patients underwent on-pump CABG with cardiopulmonary bypass (CPB) and seven patients underwent off-pump CABG without CPB. To detect .OH generation, we measured the urinary excretion of .OH products of creatinine (Cr), creatol (CTL; 5-hydroxycreatinine) and methylguanidine (MG) with HPLC using the one point sampling and collected urine during and after the operation. The urinary CTL value corrected urinary Cr value of on-pump CABG significantly increased about 3-5 times from the beginning of CPB to 4 h after operation compared to the baseline value before CPB in both the collected urine and the one point sampling urine. The urinary MG/Cr value in both groups did not change significantly. Significantly increased .OH generation was found during and soon after on-pump CABG.
Osaka
Motoo
M
Graduated School of Comprehensive Human Sciences, University of Tsukuba, and Tsukuba Medical Center Hospital, Department of Cardiovascular Surgery, Tsukuba, Japan.
Aoyagi
Kazumasa
K
Hirakawa
Akiko
A
Nakajima
Motoo
M
Jikuya
Tomoaki
T
Shigeta
Osamu
O
Sakakibara
Yuzuru
Y
D000368:Aged; D002315:Cardiopulmonary Bypass; D002851:Chromatography, High Pressure Liquid; D001026:Coronary Artery Bypass; D003404:Creatinine / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism*; D008297:Male; D008760:Methylguanidine / Q000652:urine
C064402:creatol; D017665:Hydroxyl Radical; D008760:Methylguanidine; D003404:Creatinine