title: [Effects of anti-HER-2 chimeric antibody chA21 on proliferative inhibition of SKOV3 cells and inducing their apoptosis].
abstract: OBJECTIVE
  To explore the effects of anti-HER-2 chimeric antibody chA21 on proliferation and apoptosis of ovarian cancer cell lines SKOV3.
  
  
  METHODS
  MTT colorometric assay, HE staining, transmission electron microscopy, flow cytometry and TUNEL staining were used to study the proliferative inhibition and apoptotic induction of SKOV3 cells by chA21 in vitro.
  
  
  RESULTS
  Proliferative inhibition rate and apoptotic rate of SKOV3 cells were increased with dose and action time by chA21 (0.2 mg/L-5.4 mg/L).
  
  
  CONCLUSIONS
  chA21 can remarkably inhibit proliferation of SKOV3 cells in vitro and induction of apoptosis may be a principal way.
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei 230032, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xue
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wu
authors.forename: Xiang-yang
authors.initials: XY
authors.lastname: Hu
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Ling
authors.forename: Lian-sheng
authors.initials: LS
authors.lastname: Cheng
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Ai-ping
authors.initials: AP
authors.lastname: Liu
mesh_terms: D000906:Antibodies / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D046529:Microscopy, Electron, Transmission; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000648:ultrastructure; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*
chemical_list: D000906:Antibodies; D000970:Antineoplastic Agents; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins; C508053:ERBB2 protein, human