Phosphate metabolism in the cyanobacterium Anabaena doliolum under salt stress.
In the present study, we have investigated the effects of NaCl concentrations on the growth and phosphate metabolism of an Anabaena doliolum strain isolated from a paddy field, in order to determine the possible effects of salinization. Growth rate, chlorophyll content, and protein content decreased with increasing salt concentration in the growth medium, while carbohydrate concentration increased. Phosphate content and phosphate uptake rate decreased. There was an increase in total alkaline phosphatase activity, with an approximately 7-fold increase in extracellular activity compensating for an approximately 3-fold decrease in cell-bound activity. NaCl effects on protein and chlorophyll concentrations were greater in P-deficient medium, while presence or absence of P in the medium had little effect on cellular carbohydrate concentrations. It is concluded that growth in high salt likely leads to reduced phosphate uptake in A. doliolum.
Rai
Ashwani K
AK
Department of Botany, Banaras Hindu University, Varanasi 221 005, India. akrai@bhu.ac.in
Sharma
N K
NK
D000222:Adaptation, Physiological; D000469:Alkaline Phosphatase / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis; D003470:Culture Media / Q000737:chemistry; D000458:Cyanobacteria / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D004783:Environmental Microbiology; D009997:Osmotic Pressure; D010710:Phosphates / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*
D001426:Bacterial Proteins; D002241:Carbohydrates; D003470:Culture Media; D010710:Phosphates; D002734:Chlorophyll; D012965:Sodium Chloride; D000469:Alkaline Phosphatase