[Construction of tumor cell-specific expression vector modulated by human telomerase reserse transcription gene core promoter].
OBJECTIVE
To construct the tumor cell-specific expression vector modulated by human telomerase reserse transcription gene (hTERT) core promoter.


METHODS
The hTERT gene core promoter fragment was amplified by PCR using the total genomic DNA from SKOV3 cells as template. The amplified gene fragment was subsequently cloned into pIRES2-EGFP vector. Then the expression vector modulated by hTERT core gene promoter was transfected into tumor cell lines, HO-8910, Hela and normal ECV304 cells through lipofectamine, respectively. The activity of reporter gene GFP was detected 48 h after transfection.


RESULTS
There was significant transcriptional activity in 3 kinds of transfected tumor cells, while no transcriptional activity was detected in transfected normal cells.


CONCLUSIONS
The hTERT core gene promoter has the tumor specificity, suggesting that the specific expression vector modulated by hTERT core gene promoter may be a novel and promising approach to the tumor treatment.
Li
Hong-mei
HM
Environment and Genes Related to Diseases Key Laboratory of Education Ministry, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. ylhmqq@yahoo.com.cn
Song
Tian-bao
TB
Yu
Yue-cheng
YC
Huang
Hong-yan
HY
Zhang
Ming
M
D045744:Cell Line, Tumor; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D014162:Transfection
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C509186:TERT protein, human; D019098:Telomerase