Antihyperglycemic effect of constituents from Hintonia standleyana in streptozotocin-induced diabetic rats.
An extract (100 mg/kg) of the stem bark of Hintonia standleyana caused a significant decrease in blood glucose levels in both normal and streptozotocin (STZ)-diabetic rats when compared with vehicle-treated groups (p < 0.05). From the active extract, 3- O- beta- D-glucopyranosyl-23,24-dihydrocucurbitacin F ( 1), 5- O-beta- D-glucopyranosyl-7-methoxy-3',4'-dihydroxy-4-phenylcoumarin ( 2) and 5- O-[ beta- D-apiofuranosyl-(1-->6)- beta- D-glucopyranosyl]-7-methoxy-3',4'-dihydroxy-4-phenylcoumarin ( 3) were isolated. Coumarin 3 is a new natural product and was identified by spectroscopic methods. Compounds 1 and 3 did not decrease blood glucose levels in normal rats. However, in two different long-term subacute experiments, using animals with a developing diabetes condition and with STZ-induced diabetes, both compounds at daily doses of 10 mg/kg (developing diabetes condition) or 30 mg/kg (STZ-induced diabetes condition) provoked a significant antihyperglycemic activity (p < 0.05). Furthermore, compound 3 restored normal blood glucose levels in STZ-induced diabetic rats. In all cases, the groups treated with the active principles and the extract showed less body weight lost than the glibenclamide-treated and diabetic control groups (p < 0.05). These results showed that the antihyperglycemic active principles of H. standleyana are both 4-phenylcoumarins and cucurbitacin glycosides.
Guerrero-Analco
José A
JA
Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, México DF, México.
Hersch-Martínez
Paul
P
Pedraza-Chaverri
José
J
Navarrete
Andrés
A
Mata
Rachel
R
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D004187:Disaccharides / Q000737:chemistry / Q000494:pharmacology; D006943:Hyperglycemia / Q000097:blood / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000494:pharmacology*; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry / Q000494:pharmacology; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D019666:Rubiaceae / Q000737:chemistry*; D012503:Saponins; D013997:Time Factors; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology
C512338:3-O-glucopyranosyl-23,24-dihydrocucurbitacin F; C508517:5- O-beta- D-glucopyranosyl-7-methoxy-3',4'-dihydroxy-4-phenylcoumarin; C508519:5-O-(beta- D-apiofuranosyl-(1-6)-beta-D-glucopyranosyl)-7-methoxy-3',4'-dihydroxy-4-phenylcoumarin; D001786:Blood Glucose; D003374:Coumarins; D004187:Disaccharides; D007004:Hypoglycemic Agents; D009005:Monosaccharides; D012503:Saponins; D014315:Triterpenes