Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene.
BACKGROUND
The expansion of specific trinucleotide repeats results in certain genetic disorders.


METHODS
Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene.


RESULTS
The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C.


CONCLUSIONS
Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.
Chatzikyriakidou
Anthoula
A
Genetics Unit, Department of Obstetrics and Gynaecology, Ioannina University School of Medicine, Ioannina, Greece.
Yapijakis
Christos
C
Sofikitis
Nikolaos
N
Vassilopoulos
Dimitrios
D
Georgiou
Ioannis
I
D000483:Alleles; D041321:Chromosomes, Human, X; D005260:Female; D006801:Humans; D008297:Male; D020966:Muscular Disorders, Atrophic / Q000235:genetics; D011944:Receptors, Androgen / Q000235:genetics*; D044366:Transition Temperature; D019680:Trinucleotide Repeat Expansion
C410798:AR protein, human; D011944:Receptors, Androgen