A highly efficient transformation protocol for Micro-Tom, a model cultivar for tomato functional genomics.
We report a highly efficient protocol for the Agrobacterium-mediated genetic transformation of a miniature dwarf tomato (Lycopersicon esculentum), Micro-Tom, a model cultivar for tomato functional genomics. Cotyledon explants of tomato inoculated with Agrobacterium tumefaciens (Rhizobium radiobacter) C58C1Rif(R) harboring the binary vector pIG121Hm generated a mass of chimeric non-transgenic and transgenic adventitious buds. Repeated shoot elongation from the mass of adventitious buds on selection media resulted in the production of multiple transgenic plants that originated from independent transformation events. The transformation efficiency exceeded 40% of the explants. This protocol could become a powerful tool for functional genomics in tomato.
Sun
Hyeon-Jin
HJ
Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.
Uchii
Sayaka
S
Watanabe
Shin
S
Ezura
Hiroshi
H
D004269:DNA, Bacterial / Q000235:genetics; D005822:Genetic Vectors; D023281:Genomics / Q000379:methods*; D005966:Glucuronidase / Q000378:metabolism; D018551:Solanum lycopersicum / Q000145:classification* / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics; D018520:Plant Shoots / Q000235:genetics; D030821:Plants, Genetically Modified; D012107:Research Design; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
D004269:DNA, Bacterial; C036483:T-DNA; D005966:Glucuronidase