The role of ribosomal protein L11 in class I release factor-mediated translation termination and translational accuracy.
It has been suggested from in vivo and cryoelectron micrographic studies that the large ribosomal subunit protein L11 and its N-terminal domain play an important role in peptide release by, in particular, the class I release factor RF1. In this work, we have studied in vitro the role of L11 in translation termination with ribosomes from a wild type strain (WT-L11), an L11 knocked-out strain (DeltaL11), and an L11 N terminus truncated strain (Cter-L11). Our data show 4-6-fold reductions in termination efficiency (k(cat)/K(m)) of RF1, but not of RF2, on DeltaL11 and Cter-L11 ribosomes compared with wild type. There is, at the same time, no effect of these L11 alterations on the maximal rate of ester bond cleavage by either RF1 or RF2. The rates of dissociation of RF2 but not of RF1 from the ribosome after peptide release are somewhat reduced by the L11 changes irrespective of the presence of RF3, and they cause a 2-fold decrease in the missense error. Our results suggest that the L11 modifications increase nonsense suppression at UAG codons because of the reduced termination efficiency of RF1 and that they decrease nonsense suppression at UGA codons because of a decreased missense error level.
Bouakaz
Lamine
L
Institute of Cell and Molecular Biology, Uppsala University, Sweden.
Bouakaz
Elli
E
Murgola
Emanuel J
EJ
Ehrenberg
Måns
M
Sanyal
Suparna
S
D018388:Codon, Terminator; D029968:Escherichia coli Proteins / Q000502:physiology*; D010454:Peptide Termination Factors / Q000502:physiology*; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000502:physiology*
D018388:Codon, Terminator; D029968:Escherichia coli Proteins; D010454:Peptide Termination Factors; D012269:Ribosomal Proteins; C489005:prfA protein, E coli; C489006:prfB protein, E coli; C489007:prfC protein, E coli; C027624:ribosomal protein L11