title: A novel three-dimensional computer-assisted method for a quantitative study of microvascular networks of the human cerebral cortex.
abstract: OBJECTIVE
  Detailed information on microvascular network anatomy is a requirement for understanding several aspects of microcirculation, including oxygen transport, distributions of pressure, and wall shear stress in microvessels, regulation of blood flow, and interpretation of hemodynamically based functional imaging methods, but very few quantitative data on the human brain microcirculation are available. The main objective of this study is to propose a new method to analyze this microcirculation.
  
  
  METHODS
  From thick sections of india ink-injected human brain, using confocal laser microscopy, the authors developed algorithms adapted to very large data sets to automatically extract and analyze center lines together with diameters of thousands of brain microvessels within a large cortex area.
  
  
  RESULTS
  Direct comparison between the original data and the processed vascular skeletons demonstrated the high reliability of this method and its capability to manage a large amount of data, from which morphometry and topology of the cerebral microcirculation could be derived.
  
  
  CONCLUSIONS
  Among the many parameters that can be analyzed by this method, the capillary size, the frequency distributions of diameters and lengths, the fractal nature of these networks, and the depth-related density of vessels are all vital features for an adequate model of cerebral microcirculation.
authors.affiliation: Functional Neuroimaging Laboratory, INSERM U455, CHU Purpan, Toulouse, France. fcassot@toulouse.inserm.fr
authors.forename: Francis
authors.initials: F
authors.lastname: Cassot
authors.forename: Frederic
authors.initials: F
authors.lastname: Lauwers
authors.forename: Céline
authors.initials: C
authors.lastname: Fouard
authors.forename: Steffen
authors.initials: S
authors.lastname: Prohaska
authors.forename: Valerie
authors.initials: V
authors.lastname: Lauwers-Cances
mesh_terms: D002196:Capillaries / Q000033:anatomy & histology; D002540:Cerebral Cortex / Q000033:anatomy & histology* / Q000098:blood supply*; D002560:Cerebrovascular Circulation; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods