Diverse range of small peptides associated with high-density lipoprotein.
High-density lipoproteins (HDL) were examined as potential carriers of small peptides in plasma. HDL purified by density gradient centrifugation was delipidated and fractionated by size-exclusion chromatography under denaturing conditions. By HPLC and mass spectrometry, more than 100 peptide components were found in the size range from 1000 to 5000 Da. By sequence analysis, peptides were identified as fragments of proteins such as apolipoproteins, fibrinogen, alpha1-proteinase inhibitor, and transthyretin. The results indicate that purified HDL bears a complex range of small peptides. It is unclear whether the peptides have any significant functional role as apolipopeptides, but they may represent a pathway for peptide delivery or scavenging and a significant reservoir of plasma peptides for diagnostic evaluation.
Hortin
Glen L
GL
Department of Laboratory Medicine, National Institutes of Health, Building 10, Room 2C-407, Bethesda, MD 20892, USA. ghortin@mail.cc.nih.gov
Shen
Rong-Fong
RF
Martin
Brian M
BM
Remaley
Alan T
AT
D001053:Apolipoproteins / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry; D002138:Calibration; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D008075:Lipoproteins, HDL / Q000737:chemistry*; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry*; D011228:Prealbumin / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D000515:alpha 1-Antitrypsin / Q000737:chemistry
D001053:Apolipoproteins; D001798:Blood Proteins; D008075:Lipoproteins, HDL; D010455:Peptides; D011228:Prealbumin; D000515:alpha 1-Antitrypsin; D005340:Fibrinogen