title: Performing vitreous biopsy by perfluorocarbon-perfused vitrectomy.
abstract: OBJECTIVE
  To evaluate the safety and efficacy of perfluorocarbon-perfused vitrectomy (PCPV) as a novel technique in obtaining a large undiluted vitreous biopsy.
  
  
  METHODS
  Cross-sectional interventional study.
  
  
  METHODS
  Patients with undiagnosed posterior uveitis scheduled for vitreous biopsy underwent PCPV. A syringe containing 5 ml of perfluorocarbon liquid (PCL) was connected to the infusion line. Aspiration of the central and superior vitreous was initiated with simultaneous infusion of the PCL.
  
  
  RESULTS
  Twenty eyes of 20 patients were included in this study. The mean +/- SD amount of PCL used in each eye was 4.50 +/- 0.69 ml. The volume of vitreous sample obtained in each eye was 2.25 +/- 0.413 ml. No complications occurred.
  
  
  CONCLUSIONS
  PCPV is a safe and effective method for obtaining large undiluted vitreous biopsy.
authors.affiliation: Retina Service, Asociación Para Evitar la Ceguera, Hospital Dr. Luis Sanchez Bulnes, Vicente Garcia Torres 46, San Lucas Coyoacan 04030, Coyoacan, Mexico City, Mexico. retinamex@yahoo.com
authors.forename: Hugo
authors.initials: H
authors.lastname: Quiroz-Mercado
authors.forename: Jorge
authors.initials: J
authors.lastname: Rivera-Sempertegui
authors.forename: Tamer A
authors.initials: TA
authors.lastname: Macky
authors.forename: Patricia
authors.initials: P
authors.lastname: Navarro-López
authors.forename: Lidia
authors.initials: L
authors.lastname: Griselda-Alvarez
authors.forename: Nayeli
authors.initials: N
authors.lastname: Ibarra-Ponce
authors.forename: Daniel
authors.initials: D
authors.lastname: Moreno-Páramo
mesh_terms: D000328:Adult; D001707:Biopsy, Needle / Q000379:methods*; D003430:Cross-Sectional Studies; D004322:Drainage / Q000379:methods; D005466:Fluorocarbons / Q000008:administration & dosage*; D006801:Humans; D008875:Middle Aged; D015866:Uveitis, Posterior / Q000473:pathology*; D014821:Vitrectomy / Q000379:methods*; D014822:Vitreous Body / Q000473:pathology*
chemical_list: D005466:Fluorocarbons