Hemin-mediated hemolysis in erythrocytes: effects of ascorbic acid and glutathione.
In the present work, we investigated the effect of ascorbic acid and glutathione on hemolysis induced by hemin in erythrocytes. Ascorbic acid not only enhanced hemolysis, but also induced formation of thiobarbituric acid-reactive substances in the presence of hemin. It has been shown that glutathione inhibits hemin-induced hemolysis by mediating hemin degradation. Erythrocytes depleted of glutathione became very sensitive to oxidative stress induced by hemin and ascorbic acid. H(2)O(2) was involved in hemin-mediated hemolysis in the presence of ascorbic acid. However, a combination of glutathione and ascorbic acid was more effective in inhibiting hemolysis induced by hemin than glutathione alone. Extracellular and intracellular ascorbic acid exhibited a similar effect on hemin-induced hemolysis or inhibition of hemin-induced hemolysis by glutathione. The current study indicates that ascorbic acid might function as an antioxidant or prooxidant in hemin-mediated hemolysis, depending on whether glutathione is available.
Li
Shu-De
SD
School of Life Sciences, Yunnan University, Kunming 650091, China.
Su
Yan-Dan
YD
Li
Ming
M
Zou
Cheng-Gang
CG
D001205:Ascorbic Acid / Q000008:administration & dosage*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D005978:Glutathione / Q000008:administration & dosage*; D006427:Hemin / Q000008:administration & dosage*; D006461:Hemolysis / Q000187:drug effects* / Q000502:physiology*; D006801:Humans
D004338:Drug Combinations; D006427:Hemin; D005978:Glutathione; D001205:Ascorbic Acid