title: Establishment of a 2-D human urinary proteomic map in IgA nephropathy.
abstract: Immunoglobulin A nephropathy (IgAN) is the most common form of immune complex-mediated glomerulonephritis worldwide. Although chronic renal failure develops in considerable numbers of IgAN patients, the exact etiology has not yet been clearly elucidated. To establish the urinary protein map of IgAN, we performed a urinary proteomic analysis. Thirteen patients with IgAN and 12 normal controls were recruited. Morning midstream spot urine samples were used with Centriprep ultrafiltration for concentration and desalting. 2-DE was performed and compared between IgAN and normal control, and urinary proteins were identified by MALDI-TOF MS. A large number of protein spots were identified in IgAN and normal control samples, with means of 311 spots and 174 spots, respectively. Approximately 216 protein spots were detected as differentially expressed in IgAN. Among these, 82 spots were over-expressed, and 134 spots were under-expressed compared to normal controls. A total of 84 differentially expressed spots, representing 59 different proteins, were finally identified in IgAN. We have established a urinary proteomic map of IgAN and this result helps in the identification. Further study is needed to determine the potential pathogenic role of these proteins.
authors.affiliation: Department of Clinical Pathology, The Catholic University of Korea, Seoul, Korea.
authors.forename: Mi-Ra
authors.initials: MR
authors.lastname: Park
authors.forename: Eun-Hui
authors.initials: EH
authors.lastname: Wang
authors.forename: Dong-Chan
authors.initials: DC
authors.lastname: Jin
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Cha
authors.forename: Kweon-Heang
authors.initials: KH
authors.lastname: Lee
authors.forename: Chul-Woo
authors.initials: CW
authors.lastname: Yang
authors.forename: Chang-Suk
authors.initials: CS
authors.lastname: Kang
authors.forename: Yeong-Jin
authors.initials: YJ
authors.lastname: Choi
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000652:urine*; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005922:Glomerulonephritis, IGA / Q000175:diagnosis* / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010449:Peptide Mapping; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers