Chromosomal imbalances in malignant peripheral nerve sheath tumor detected by metaphase and microarray comparative genomic hybridization.
Malignant peripheral nerve sheath tumors (MPNSTs) are highly malignant tumors affecting adolescents and adults. There have been a few reports on chromosomal aberrations of MPNSTs; however, the tumor-specific alteration remains unknown. We characterized the genomic alterations in 8 MPNSTs and 8 schwannomas by metaphase comparative genomic hybridization (CGH). In 5 of 8 MPNSTs, microarray CGH was added for more detailed analyses. Frequent gains were identified on 3q13-26, 5p13-14, and 12q11-23 and frequent losses were at 1p31, 10p, 11q24-qter, 16, and 17. Microarray CGH revealed frequent gains of EGFR, DAB2, MSH2, KCNK12, DDX15, CDK6, and LAMA3, and losses of CDH1, GLTSCR2, EGR1, CTSB, GATA3, and SULT2A1. These genes seem to be responsible for developing MPNSTs. The concordance rate between metaphase CGH and microarray CGH was 66%. Metaphase CGH was useful for identifying chromosomal alterations before applying microarray CGH.
Nakagawa
Yasuko
Y
Science of Functional Recovery and Reconstruction, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama 700-8558, Japan.
Yoshida
Aki
A
Numoto
Kunihiko
K
Kunisada
Toshiyuki
T
Wai
Daniel
D
Ohata
Norihide
N
Takeda
Ken
K
Kawai
Akira
A
Ozaki
Toshifumi
T
D000328:Adult; D000368:Aged; D002648:Child; D002869:Chromosome Aberrations; D005260:Female; D018628:Gene Dosage; D006801:Humans; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D018317:Nerve Sheath Neoplasms / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D012983:Soft Tissue Neoplasms / Q000235:genetics* / Q000473:pathology