Reliability in estimates of body composition of birds: oxygen-18 versus deuterium dilution.
Body composition in birds was evaluated indirectly by 18O and 2H dilution. Body composition was determined by whole-body chemical analysis of eight adult roosters (Gallus gallus). In vivo measurements of total body water (TBW) were carried out using doubly labeled water (2H2 18O). Estimated dilution spaces using both the plateau and intercept approaches were compared with the results obtained by carcass lyophilization. Both 18O and 2H slightly overestimated TBW compared with the results obtained by lyophilization, by 2.2%+/-1.9% and 5.7%+/-0.2%, respectively; both differences were statistically significant (P<0.01). The difference between these isotope estimations was significant (P<0.001). However, isotope dilution spaces and TBW were highly correlated. There was a strong inverse relationship between total body fat and TBW percentages (r2=0.98, P<0.0001). The relation between TBW and body protein was significant. Water content in lean body mass (72.8%) obtained in our study was very close to that reported in mammals, demonstrating no fundamental difference in tissue water content between birds and mammals. Estimated body fat and protein values from isotopic dilution did not significantly differ from values obtained by direct chemical analysis (P>0.05), except for body fat in the Pace and Rathbun approach (Table 3). Although estimation of TBW and body composition by isotope dilution is time consuming and expensive, deuterium offers a reliable and low-cost alternative compared with 18O. The advantage of in vivo estimation of TBW with isotopic dilution in combination with the regression approach is that it permits repeated measurements of body composition on the same birds under laboratory and free-living conditions.
Mata
Astolfo J
AJ
Centre d'Ecologie et Physiologie Energetiques, Centre National de la Recherche Scientifique-Unite Propre de Recherche 9010, 23 rue Becquerel, F-67087 Strasbourg, Cedex 2, France. amata@ivc.ve
Caloin
Michel
M
Robin
Jean-Patrice
JP
Le Maho
Yvon
Y
D000818:Animals; D001823:Body Composition; D001834:Body Water; D002645:Chickens; D003903:Deuterium; D006859:Hydrogen / Q000032:analysis*; D007201:Indicator Dilution Techniques; D008297:Male; D010100:Oxygen / Q000032:analysis*; D010103:Oxygen Isotopes; D015203:Reproducibility of Results
D010103:Oxygen Isotopes; D006859:Hydrogen; D003903:Deuterium; D010100:Oxygen