[Effects of TNF-alpha on angiogenesis in CT-26 mouse colon carcinoma homograft model].
OBJECTIVE
Tumor necrosis factor-alpha (TNF-alpha) exerts its anti-tumor effect through direct cytotoxicity on tumor cells and damage to the tumor vasculature. However, its role in tumor angiogenesis is controversial. We evaluated the angiogenic effect of TNF-alpha on BALB/c mouse colon carcinoma homograft model.


METHODS
Ten BALB/c mice were inoculated intraperitoneally with CT-26 mouse colon carcinoma cells. After a week, recombinant mouse TNF-alpha (2 microg/mL) were given four times on every other day to five animals and the same volume of phosphate buffered saline was given at the same interval to five animals as control. Harvested tumor tissues were stained by immunohistochemistry with CD31 and VEGF antibodies. Number of microvessels and VEGF expression were counted by light microscope.


RESULTS
The mean microvessel counts per 200x field of TNF-alpha treated animals were 70.2+/-7.8 and those of nontreated animals were 83.8+/-8.3 (p<0.05). The VEGF score of both groups were 3.


CONCLUSIONS
TNF-alpha treated animals showed decreased microvessel counts in tumor tissue but VEGF expression in both groups showed no difference. Therefore, TNF-alpha showed antiangiogenic effects on colon carcinoma homograft model.
Chun
Jong Woon
JW
Departments of Internal Medicine, Catholic University of Daegu School of Medicine, Daegu, Korea.
Jung
Jin Tae
JT
Oh
Hoon Kyu
HK
Bae
Jong Yup
JY
Shin
Im Hee
IH
Cho
Chang Ho
CH
D000818:Animals; D002277:Carcinoma / Q000737:chemistry / Q000503:physiopathology*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000737:chemistry / Q000503:physiopathology*; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic / Q000503:physiopathology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A