Comparative analysis of expressed sequence tags (ESTs) of ginseng leaf.
The expressed sequence tags (ESTs) referenced in this report are the first transcriptomes in a leaf from a half-shade ginseng plant. A cDNA library was constructed from samples of the leaves of 4-year-old Panax ginseng plants, which were cultured in a field. The 2,896 P. ginseng cDNA clones represent 1,576 unique sequences, consisting of 1,167 singletons and 409 contig sequences. BLAST comparisons of the cDNAs in GenBank's non-redundant databases revealed that 2,579 of the 2,896 cDNAs (89.1%) exhibited a high degree of sequence homology to genes from other organisms. The majority of the identified transcripts were found to be genes related with energy, metabolism, subcellular localization, and protein synthesis and transport. The chlorophyll a/b-binding protein ESTs in the ginseng leaf samples manifested a substantially higher level of expression than was observed in other plant leaves. The ESTs involved in ginsenoside biosynthesis were also identified and discussed.
Kim
Myung Kyum
MK
Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung-eup Yongin, Kyunggi-do 449-701, South Korea.
Lee
Bum-Soo
BS
In
Jun-Gyo
JG
Sun
Hua
H
Yoon
Jae-Ho
JH
Yang
Deok-Chun
DC
D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015723:Gene Library; D036145:Ginsenosides / Q000378:metabolism*; D005894:Panax / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism
D036145:Ginsenosides; D018749:RNA, Plant