A unimolecular G-quadruplex that functions as a synthetic transmembrane Na+ transporter.
We describe the covalent post-modification of a hydrogen-bonded assembly with the subsequent formation of a potent transmembrane Na+ ion transporter. Olefin metathesis is used to cross-link all 16 guanosine subunits in a lipophilic G-quadruplex. The resulting unimolecular G-quadruplex folds in the environment of a phospholipid membrane and functions as a Na+ ion transporter as judged by fluorescence and 23Na NMR transport assays.
Kaucher
Mark S
MS
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Harrell
William A
WA
Davis
Jeffery T
JT
D002414:Cations, Monovalent; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D017136:Ion Transport; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008567:Membranes, Artificial; D019906:Nuclear Magnetic Resonance, Biomolecular; D012964:Sodium / Q000737:chemistry* / Q000378:metabolism
D002414:Cations, Monovalent; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D006147:Guanine; D004247:DNA; D012964:Sodium