The effects of heat treatments on ectomycorrhizal resistant propagules and their ability to colonize bioassay seedlings.
The effect of disturbance on the resistant propagule community (RPC) of ectomycorrhizal fungi has been given relatively little attention. In this study we investigate the effects of heat, one important factor of fire disturbances, on the ability of ectomycorrhizal RPC fungi to colonize Pinus jeffreyi seedlings in greenhouse bioassays. Prior to planting the seed, soils were collected from an old growth mixed-conifer forest in the southern Sierra Nevada, California, USA and then subjected to four heat treatments of none, 45 degrees C, 60 degrees C, and 75 degrees C. After eight months, seedlings were harvested and the ectomycorrhizal fungi colonizing the roots were characterized by molecular methods (PCR-RFLP and DNA sequencing). Rhizopogon species increased in dominance on seedlings grown in soils receiving the 75 degrees C heat treatment. One species significantly increased in frequency, Rhizopogon olivaceotinctus, and two species (Cenococcum geophilum and Wilcoxina sp.) significantly decreased in frequency in the 75 degrees C treatment. The increase of R. olivaceotinctus, coupled with other features of its behavior, suggests that substantial heat disturbances may benefit this species in competing for roots.
Izzo
Antonio
A
University of California at Berkeley, Department of Plant and Microbial Biology, 321 Koshland Hall, Berkeley, CA 94720-3102, USA. izzo@tfrl.ars.usda.gov
Canright
Megan
M
Bruns
Thomas D
TD
D001487:Basidiomycota / Q000235:genetics / Q000254:growth & development*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D005390:Fires; D006358:Hot Temperature; D028223:Pinus / Q000254:growth & development / Q000382:microbiology*; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012340:RNA, Ribosomal, 5.8S / Q000737:chemistry / Q000235:genetics; D036226:Seedlings / Q000254:growth & development / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004271:DNA, Fungal; D021901:DNA, Intergenic; D012340:RNA, Ribosomal, 5.8S