Production of mycelia and exo-biopolymer from molasses by Cordyceps sinensis 16 in submerged culture.
The molecular weight of exo-biopolymer obtained from a submerged culture of Cordyceps sinensis 16 consisted of a main unit and a subunit of 126 and 68 kDa, respectively. The optimal medium for the production of mycelia and exo-biopolymer was determined to be molasses containing 2% sucrose, 0.9% yeast extract, 0.3% K2HPO4, and 0.4% CaCl2. Using optimized medium, maximum productions of mycelia and exo-biopolymer in shake-flask culture were 54.0 g/L and 28.4 g/L, respectively. This study suggests that large-scale production of mycelia and exo-biopolymer by C. sinensis 16 is possible in submerged culture.
Cha
S H
SH
Department of Chemical and Biological Engineering, Korea University, 5-1 Anam-dong, Sungbuk-gu, Seoul 136-701, Republic of Korea.
Lim
J S
JS
Yoon
C S
CS
Koh
J H
JH
Chang
H I
HI
Kim
S W
SW
D001704:Biopolymers / Q000378:metabolism*; D032661:Cordyceps / Q000378:metabolism*; D003470:Culture Media; D008965:Molasses; D025282:Mycelium / Q000254:growth & development*; D013997:Time Factors
D001704:Biopolymers; D003470:Culture Media