Comparison of active venom components between Eastern brown snakes collected from South Australia and Queensland.
The abundance and activity of the prothrombin activator (pseutarin C) within the venom of the Eastern brown snake (Pseudonaja textilis textilis) is the primary determinant of its coagulation potency. Textilinin-1, also in this venom, is a plasmin inhibitor which is thought to exert its toxic effects through the slowing of fibrinolysis. The aim of this report is to determine if there are differences in the potency of the venom from Eastern brown snakes collected from South Australia (SA) compared to those from Queensland (QLD). A concentration of 0.4 microg/ml venom protein from six QLD specimens clotted citrated plasma in an average time of 21.4+/-3.3 s compared to 68.7+/-2.4 s for the same amount of SA venom (averaged for six individuals). The more potent procoagulant activity of the QLD venom was measured between 0.4 and 94 microg/ml venom protein in plasma. The anti-plasmin activity of textilinin was also greater in the venom of the snakes collected from QLD, causing full inhibition of plasmin at approximately 1.88 microg/ml of venom protein compared to approximately 7.5 microg/ml for the SA venoms. It is concluded that geographic differentiation of the Eastern brown snakes results in significant differences venom potency.
Flight
Simone
S
Level 2, Research Wing, Princess Alexandra Hospital, Woolloongabba, 4012, Brisbane, QLD, Australia. sflight@soms.uq.edu.au
Mirtschin
Peter
P
Masci
Paul P
PP
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D004546:Elapid Venoms / Q000032:analysis / Q000494:pharmacology*; D017815:Elapidae; D015951:Factor Xa / Q000378:metabolism; D005341:Fibrinolysin / Q000037:antagonists & inhibitors*; D006801:Humans; D011793:Queensland; D013021:South Australia; D013045:Species Specificity
D004546:Elapid Venoms; C475049:Pseutarin C; C413544:textilinin; D015951:Factor Xa; D005341:Fibrinolysin