title: Adsorption of biopolyester depolymerase on silicon wafer and poly[(R)-3-hydroxybutyric acid] single crystal revealed by real-time AFM.
abstract: The adsorption behavior of PHB depolymerase from R. pickettii T1 on a silicon wafer and on P(3HB) single crystals has been studied by real-time and AFM in air and a buffer solution. First, the morphology of PHB depolymerase adsorbed on a silicon wafer was characterized to show that one molecule of PHB depolymerase has dimensions of 2.2 +/- 0.7 nm height and 16 +/- 5 nm width. The observation of PHB depolymerase adsorbed on a P(3HB) single crystal indicated that the dimensions of enzyme on the crystalline surface in air were 1.2 +/- 0.5 nm high and 28 +/- 7 nm wide, while enzyme molecules with dimensions of 2.1 +/- 0.6 nm height and 16 +/- 7 nm width were detected in a buffer solution. Comparison of the dimensions of PHB depolymerase in air with those in a buffer solution showed that the enzyme was squashed in air, but not in a buffer solution. In addition, the influence of enzymatic adsorption on the molecular state of the P(3HB) crystalline surface was investigated. The AFM images of P(3HB) single crystals after enzymatic adsorption and washing with ethanol indicated that the adhesion of PHB depolymerase changed the molecular state and generated holes on the crystalline surface.
authors.affiliation: Department of Innovative and Engineered Materials, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8502, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Numata
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kikkawa
authors.forename: Takeharu
authors.initials: T
authors.lastname: Tsuge
authors.forename: Tadahisa
authors.initials: T
authors.lastname: Iwata
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Doi
authors.forename: Hideki
authors.initials: H
authors.lastname: Abe
mesh_terms: D000327:Adsorption; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D003460:Crystallization; D006885:Hydroxybutyrates / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011091:Polyesters / Q000737:chemistry*; D012825:Silicon; D017550:Spectroscopy, Fourier Transform Infrared; D000094122:Polyhydroxybutyrates
chemical_list: D002265:Carboxylic Ester Hydrolases; D006885:Hydroxybutyrates; D011091:Polyesters; D012825:Silicon; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C022417:poly-beta-hydroxybutyrate depolymerase