Targeted cleavage of HIV rev response element RNA by metallopeptide complexes.
Stoichiometric targeting and site-specific cleavage of HIV RRE RNA is demonstrated under physiologically relevant conditions by use of a metallopeptide that combines a specific RNA recognition sequence with a metal binding domain. Mass spectrometric analysis of cleavage products following treatment of target RNA with the metallopeptide, ascorbate, and dioxygen are consistent with C-1'H or C-4'H oxidative cleavage paths with an apparent second-order rate constant k2 approximately 700 M-1 min-1.
Jin
Yan
Y
Evans Laboratory of Chemistry, The Ohio State University, Columbus, 43210, USA.
Cowan
J A
JA
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D015694:Gene Products, rev / Q000737:chemistry* / Q000378:metabolism; D015752:Genes, env; D006678:HIV / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D008667:Metalloproteins / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry* / Q000378:metabolism; D020218:Response Elements; D013379:Substrate Specificity; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D008667:Metalloproteins; D010446:Peptide Fragments; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus; D003300:Copper