Aging influences multiple incidices of oxidative stress in the aortic media of the Fischer 344/NNiaxBrown Norway/BiNia rat.
Here, we determine the influence of aging on multiple markers of oxidative stress in the aorta of adult (6-month), aged (30-month) and very aged (36-month) Fischer 344/NNiaHSdxBrown Norway/BiNia (F344/NxBN) rats. Compared to adults, increases in as determined by oxidation of hydroethidine (HE) to ethidium (Et) were increased 79.7+/-7.0% in 36-month aortae and this finding was highly correlated with increases in medal thickness (r=0.773, p<0.01) and total protein nitration (r=0.706, p<0.01) but not Ki67, a marker for cell proliferation. Regression analysis showed that increases in aortic superoxide anion (O.-2) with aging were significantly correlated with changes in the expression and/or regulation of proteins involved in metabolic (AMPK-alpha), signaling (mitogen activated protein kinases (MAPKs) along with c-Src), apoptotic (Bax, Bcl-2, Traf-2) and transcriptional (NF-kappaB) activities. These results suggest that the aging F344/NxBN aorta may be highly suited for unraveling the molecular events that lead to age-associated alterations in aortic oxidative stress.
Rice
K M
KM
Marshall University, Department of Biological Sciences, Huntington, WV 2755-1090, USA.
Preston
D L
DL
Walker
E M
EM
Blough
E R
ER
D055372:AMP-Activated Protein Kinases; D000375:Aging / Q000502:physiology*; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D049109:Cell Proliferation; D004996:Ethidium / Q000737:chemistry; D016391:Genes, src; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009097:Multienzyme Complexes; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D010617:Phenanthridines / Q000737:chemistry; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D047992:TNF Receptor-Associated Factor 2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D019394:Ki-67 Antigen; D009097:Multienzyme Complexes; D016328:NF-kappa B; D010617:Phenanthridines; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D047992:TNF Receptor-Associated Factor 2; D051028:bcl-2-Associated X Protein; C058428:hydroethidine; D017346:Protein Serine-Threonine Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D055372:AMP-Activated Protein Kinases; D004996:Ethidium