title: Rotation of the myocardial wall of the outflow tract is implicated in the normal positioning of the great arteries.
abstract: Congenital heart defects frequently involve a failure of outflow tract (OFT) formation during development. We analyzed the remodeling of the OFT, using the y96-Myf5-nlacZ-16 transgene, which marks a subpopulation of myocardial cells of the pulmonary trunk. Expression analyses of reporter transcript and protein suggest that the myocardial wall of the OFT rotates before and during the formation of the great arteries. Rotational movement was confirmed by Di-I injection experiments with cultured embryos. We subsequently examined the expression of the transgene in mouse models for OFT defects. In hearts with persistent truncus arteriosus (PTA), double outlet right ventricle (DORV), or transposition of the great arteries, rotation of the myocardial wall of the OFT is arrested or fails to initiate. This is observed in Splotch (Pax3) mutants with PTA or DORV and may be a result of defects in neural crest migration, known to affect OFT septation. However, in Pitx2deltac mutant embryos, where cardiac neural crest cells are present in the heart, PTA and DORV are again associated with a rotation defect. This is also seen in Pitx2deltac mutants, which have transposition of the great arteries. Because Pitx2c is involved in left-right signaling, these results suggest that embryonic laterality affects rotation of the myocardial wall during OFT maturation. We propose that failure of normal rotation of OFT myocardium may underlie major forms of congenital heart disease.
authors.affiliation: Department of Developmental Biology, CNRS URA 2578, Pasteur Institute, Paris, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Bajolle
authors.forename: Stéphane
authors.initials: S
authors.lastname: Zaffran
authors.forename: Robert G
authors.initials: RG
authors.lastname: Kelly
authors.forename: Juliette
authors.initials: J
authors.lastname: Hadchouel
authors.forename: Damien
authors.initials: D
authors.lastname: Bonnet
authors.forename: Nigel A
authors.initials: NA
authors.lastname: Brown
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Buckingham
mesh_terms: D000818:Animals; D001011:Aorta / Q000033:anatomy & histology* / Q000502:physiology*; D017931:DNA Primers; D004195:Disease Models, Animal; D006321:Heart / Q000033:anatomy & histology* / Q000502:physiology*; D006330:Heart Defects, Congenital / Q000196:embryology* / Q000473:pathology; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D051787:Myogenic Regulatory Factor 5 / Q000172:deficiency / Q000235:genetics; D016133:Polymerase Chain Reaction; D012399:Rotation
chemical_list: D017931:DNA Primers; C497418:Myf5 protein, mouse; D051787:Myogenic Regulatory Factor 5