STAT6 specific shRNA inhibits proliferation and induces apoptosis in colon cancer HT-29 cells.
RNA interference (RNAi) has been employed as an effective tool for studying gene functions. Here, we have demonstrated that different short hairpin RNAs (shRNAs) can specifically knock-down the transcription of STAT6, resulting in the inhibition of cell proliferation, G1/S phase arrest and apoptotic death in colon cancer HT-29 cells. These findings suggest that Stat6 signaling pathway plays a role in the regulation of cell proliferation and apoptosis in colon cancer cells, which promises further investigation for targeted cancer therapy.
Zhang
Mingsheng
M
Department of Cancer Radio-Chemotherapy, Wuhan University Zhongnan Hospital and Cancer Research Center, 169 Donghu Road, Wuhan, Hubei 430071, China. zms75@163.com <zms75@163.com>
Zhou
Yunfeng
Y
Xie
Conghua
C
Zhou
Fuxiang
F
Chen
Yun
Y
Han
Guang
G
Zhang
Wen Jie
WJ
D017209:Apoptosis / Q000235:genetics* / Q000502:physiology; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology; D005434:Flow Cytometry; D019073:HT29 Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics*; D050800:STAT6 Transcription Factor / Q000235:genetics* / Q000502:physiology; D014162:Transfection
D012330:RNA, Double-Stranded; D050800:STAT6 Transcription Factor