Molecular cloning of canine mucosal addressin cell adhesion molecule-1 cDNA and its expression in normal tissues.
A cDNA encoding canine mucosal addressin cell adhesion molecule-1 (MAdCAM-1) was cloned. The entire open reading frame of canine MAdCAM-1 cDNA comprises 1137 bp, corresponding to 378 amino acid residues. The deduced amino acid sequence of canine MAdCAM-1 was 55.2%, 53.7%, and 52.4% identical to rat, mouse, and human MAdCAM-1, respectively. Canine MAdCAM-1 appeared to contain two immunoglobulin-like domains at the N-terminus, followed by a mucin-like domain and a third immunoglobulin-like domain. The structures of the dog, rat, and mouse proteins are likely similar because all of the cysteine residues in the immunoglobulin-like domains were conserved. Canine MAdCAM-1 mRNA was confirmed to express extremely in the mesenteric lymph node by RT-PCR.
Miura
Teruhisa
T
Laboratory of Comprehensive Veterinary Clinical Studies, Department of Veterinary Medicine, Nihon University, Kanagawa, Japan.
Watari
Toshihiro
T
Maruyama
Haruhiko
H
Sakai
Manabu
M
Takahashi
Tomoko
T
Kano
Rui
R
Koie
Hiroshi
H
Yamaya
Yoshiki
Y
Tokuriki
Mikihiko
M
Hasegawa
Atsuhiko
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs / Q000235:genetics* / Q000378:metabolism; D007136:Immunoglobulins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D015815:Cell Adhesion Molecules; D017931:DNA Primers; D018076:DNA, Complementary; D007136:Immunoglobulins; D009088:Mucoproteins