Measurement of monomolecular binding constants of neutral phenols into the beta-cyclodextrin by continuous frontal analysis in capillary and microchip electrophoresis via a competitive assay.
Measurement of binding constant by chip electrophoresis is a very promising technique for the high throughput screening of non-covalent interactions. Among the different electrophoretic methods available that yield the binding parameters, continuous frontal analysis is the most appropriate for a transposition from capillary electrophoresis (CE) to microchip electrophoresis. Implementation of this methodology in microchip was exemplified by the measurement of inclusion constants of 2-naphtalenesulfonate and neutral phenols (phenol, 4-chlorophenol and 4-nitrophenol) into beta-cyclodextrin by competitive assays. The issue of competitor choice is discussed in relation to its appropriateness for proper monitoring of the interaction.
Le Saux
Thomas
T
Graduate School of Material Science, University of Hyogo, Kamigori, Hyogo 697-1297 Japan. Thomas.LeSaux@ens.fr
Hisamoto
Hideaki
H
Terabe
Shigeru
S
D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D010636:Phenols / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D010636:Phenols; D047392:beta-Cyclodextrins; C031215:betadex