[Production of active oxygen by neutrophils in patients with cancer of the esophagus and stomach before and after surgical interventions].
The state of the oxygen-depended antimicrobial activity of neutrophils on oncologic patients was studied. Production of active oxygen by non-activated and activated neutrophils of peripheral blood from 19 patients (the average age of 56 years, 16 males and 3 females) with esophageal cancer and cancer of the stomach and metastasis to the esophagus was recorded by chemiluminescence before and after surgical interventions (transpleural gastrectomy, Lewis type operation) on the 1st, 3rd and 7th days after the operations. The oxygen-depended antimicrobial activity of the neutrophils was investigated by their ability to produce active oxygen when incubated with opsonized zymosan and N-formyl-methionyl-leucyl-phenylalanine. Luminol chemiluminescence recorded the whole pool of active oxygen and showed the summary activity of myeloperoxidase and NADPH-oxidase, while luceginin chemiluminescence measured formation of superoxide anion radical (*O(-)2) and evaluated the activity of NADPH-oxidase. The results showed that the procedure provided estimation of the functional state of the neutrophil leukocytes and their ability to produce active oxygen. Impairment of the myeloperoxidase- and NADPH-depended production of active oxygen by non-activated and activated neutrophils observed before operations resulted in altered synthesis of active oxygen after the surgery. This is one of the leading factors of the infection development in the end. Detection of the myeloperoxidase- and NADPH-depended production of active oxygen could be of help in revealing the groups of risk among patients at the stage of the preoperative examination and serve as a prognostic factor of the development of severe infectious complications during the postoperative period.
Nikiforova
Z N
ZN
Shevchenko
V E
VE
Volkova
Z V
ZV
Gudoshnikova
O V
OV
Dmitrieva
N V
NV
Arnotskaia
N E
NE
D001424:Bacterial Infections / Q000175:diagnosis* / Q000276:immunology; D004938:Esophageal Neoplasms / Q000276:immunology / Q000601:surgery*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000032:analysis; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D009195:Peroxidase / Q000032:analysis; D011183:Postoperative Complications / Q000175:diagnosis* / Q000276:immunology; D011379:Prognosis; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012306:Risk; D013274:Stomach Neoplasms / Q000276:immunology / Q000601:surgery*; D015054:Zymosan / Q000494:pharmacology
D017382:Reactive Oxygen Species; D015054:Zymosan; D009195:Peroxidase; D019255:NADPH Oxidases