Aged garlic extract induces proliferation and ameliorates gentamicin-induced toxicity in LLC-PK1 cells.
Gentamicin (GM)-induced nephrotoxicity limits the use of this antibiotic. It has been shown that aged garlic extract (AGE) and S-allylcysteine (SAC), the most abundant organosulfur compound in AGE, ameliorate GM-induced nephrotoxicity in rats. The present communication evaluated the effect of AGE and SAC on proliferation and on GM-induced toxicity and genotoxicity of porcine kidney epithelial cell line (LLC-PK1 cells). The cells were preincubated with different concentrations of AGE or SAC for 12 h before incubation with 8 mm GM for an additional 72 h. At the end of this time, cell viability, genotoxicity and proliferation were evaluated. AGE stimulated cell proliferation and protected LLC-PK1 cells from GM-mediated toxicity and genotoxicity. SAC partially prevented only GM-induced genotoxicity. These results suggest that the stimulation of cell proliferation could possibly be one of the mechanisms involved in the in vitro protective effect of AGE in GM-induced toxicity of LLC-PK1 cells.
Velasco-Velázquez
Marco A
MA
Departamento de Farmacología, Facultad de Medicina, UNAM, Mexico City, Mexico.
Maldonado
Perla D
PD
Barrera
Diana
D
Torres
Víctor
V
Zentella-Dehesa
Alejandro
A
Pedraza-Chaverrí
José
J
D000704:Analysis of Variance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004797:Enzyme-Linked Immunosorbent Assay; D005737:Garlic / Q000737:chemistry; D005839:Gentamicins / Q000633:toxicity*; D018374:LLC-PK1 Cells; D009152:Mutagenicity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D018675:Toxicity Tests
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D010936:Plant Extracts; D020011:Protective Agents; C065299:S-allylcysteine; D001973:Bromodeoxyuridine; D003545:Cysteine