Isolation and characterization of a putative anthocyanidin reductase gene from Ginkgo biloba.
We isolated a putative anthocyanidin reductase gene from Ginkgo biloba (designated as GbANR) by rapid amplification of cDNA ends (RACE). The full-length cDNA of GbANR is 1451 bp long with poly(A) tail and contains a 1026 bp open reading frame (ORF) encoding 342 amino acids. The GbANR gene is composed of five exons and four introns. Sequence alignment shows that the deduced GbANR has a relatively high identity to other plant anthocyanidin reductases at the amino acid level. DNA gel blot analysis indicates that GbANR belongs to a small gene family. Transcription analysis indicates that GbANR is preferentially expressed in leaves.
Shen
Guo-An
GA
Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200030, PR China.
Pang
Yongzhen
Y
Wu
Weisheng
W
Liu
Xuefen
X
Zhao
Lingxia
L
Sun
Xiaofen
X
Tang
Kexuan
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D017343:Genes, Plant; D020441:Ginkgo biloba / Q000235:genetics*; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics*; D016415:Sequence Alignment
C510059:anthocyanidin reductase, Ginkgo biloba; D009247:NADH, NADPH Oxidoreductases