Species-specific polymerase chain reaction amplification of camel (Camelus) DNA extracts.
A sensitive polymerase chain reaction (PCR) method based on amplification of a specific DNA fragment was established for the identification of camel (Camelus) materials. The species-specific primer pair L183/H372 was designed based on the nucleotide sequence of the mitochondrial cytochrome b gene, and its specificity was confirmed by amplification of 3 camel (domestic double-humped camel, wild double-humped camel, wild one-humped camel) samples and 11 non-Camelus animal (sheep, goat, pig, chicken, cattle, fish, dog, horse, donkey, deer, and rabbit) materials. An expected 208 base pair fragment was amplified from camel materials; no cross-reactive or additional fragments were generated from other animal materials. Taq I restriction endonuclease digestion of the unpurified PCR product can be used routinely to confirm the camel origin of the amplified sequence.
Chen
Ying
Y
Institute of Food Safety, Chinese Academy of Inspection and Quarantine, No. 3, Gaobeidian North Rd, Chaoyang District, Beijing, 100025 People's Republic of China. yqychen@yahoo.com.cn
Wu
Yajun
Y
Xu
Bao-Liang
BL
Wan
Jing
J
Qian
Zeng-Ming
ZM
D000818:Animals; D002162:Camelus / Q000145:classification* / Q000235:genetics*; D002417:Cattle; D045303:Cytochromes b / Q000235:genetics*; D004247:DNA / Q000145:classification; D017931:DNA Primers; D004285:Dogs; D005504:Food Analysis / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D004247:DNA; D045303:Cytochromes b