title: [Detection of proinflammatory cytokine levels in sera from patients with Kaschin-Beck disease].
abstract: OBJECTIVE
  To explore the role of proinflammatory cytokines (TNF, IL-1beta and IL-6) in the pathogenesis of Kaschin-Beck disease (KBD).
  
  
  METHODS
  The levels of serum TNF, IL-1beta and IL-6 from 62 KBD patients and 60 healthy persons were detected by double antibody sandwich ELISA.
  
  
  RESULTS
  The levels of serum IL-1beta and IL-6 in KBD patients were (238.4+/-698.5) ng/L and (164.4+/-661.4) ng/L, respectively, but they were higher than those in healthy persons, being (74.5+/-130.0) ng/L and (52.2+/-154.6) ng/L, respectively. There were no significant differences between them (P>0.05). However, the level of serum TNF in KBD patients [(109.2+/-145.3) ng/L] was significantly higher than that in healthy persons [(40.9+/-89.7) ng/L] (P<0.01). Moreover, there was no correlation between serum TNF and IL-1beta levels (r=0.0387, P>0.05) and TNF and IL-6 in KBD patients (r=0.2135, P>0.05), but there was positive correlation between serum IL-1beta and IL-6 levels (r=0.346, P<0.01).
  
  
  CONCLUSIONS
  The elevated proinflammatory cytokine levels in sera may relate to the pathogenesis of Kaschin-Beck disease.
authors.affiliation: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Can-sheng
authors.initials: CS
authors.lastname: Zhu
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Feng
authors.forename: Yue-xiang
authors.initials: YX
authors.lastname: Yu
authors.forename: Dong-lin
authors.initials: DL
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Wang
authors.forename: Bo-quan
authors.initials: BQ
authors.lastname: Jin
mesh_terms: D000328:Adult; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008875:Middle Aged; D010003:Osteoarthritis / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha