Triplex formation with alpha-L-LNA (alpha-L-ribo-configured locked nucleic acid).
Using UV melting and CD spectroscopy, we show that alpha-l-LNA-modified oligonucleotides possess the ability to form triplexes at pH 6.8 with significantly increased thermostability relative to DNA triplexes.
Kumar
Niti
N
Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, 5230 Odense M, Denmark.
Nielsen
Katrine E
KE
Maiti
Souvik
S
Petersen
Michael
M
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*
D009696:Nucleic Acids; D009841:Oligonucleotides; C051802:triplex DNA; D004247:DNA