title: The REFOLD database: a tool for the optimization of protein expression and refolding.
abstract: A large proportion of proteins expressed in Escherichia coli form inclusion bodies and thus require renaturation to attain a functional conformation for analysis. In this process, identifying and optimizing the refolding conditions and methodology is often rate limiting. In order to address this problem, we have developed REFOLD, a web-accessible relational database containing the published methods employed in the refolding of recombinant proteins. Currently, REFOLD contains >300 entries, which are heavily annotated such that the database can be searched via multiple parameters. We anticipate that REFOLD will continue to grow and eventually become a powerful tool for the optimization of protein renaturation. REFOLD is freely available at http://refold.med.monash.edu.au.
authors.affiliation: Department of Biochemistry and Molecular Biology, PO Box 13D, Monash University, Victoria 3800, Australia.
authors.forename: Michelle K M
authors.initials: MK
authors.lastname: Chow
authors.forename: Abdullah A
authors.initials: AA
authors.lastname: Amin
authors.forename: Kate F
authors.initials: KF
authors.lastname: Fulton
authors.forename: Thushan
authors.initials: T
authors.lastname: Fernando
authors.forename: Lawrence
authors.initials: L
authors.lastname: Kamau
authors.forename: Chris
authors.initials: C
authors.lastname: Batty
authors.forename: Michael
authors.initials: M
authors.lastname: Louca
authors.forename: Storm
authors.initials: S
authors.lastname: Ho
authors.forename: James C
authors.initials: JC
authors.lastname: Whisstock
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Bottomley
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Buckle
mesh_terms: D030562:Databases, Protein; D015870:Gene Expression; D020407:Internet; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism*; D014584:User-Computer Interface
chemical_list: D011994:Recombinant Proteins