title: Effect of interleukin-8 on histamine release from totally isolated vascularly perfused rat stomach.
abstract: BACKGROUND
  Recent studies have demonstrated relationships between cytokines and gastric acid secretion. The present study was performed in rats to elucidate the effects of interleukin-8 (IL-8) on gastric acid secretion through an increase in histamine release from the stomach.
  
  
  METHODS
  The experiments were performed in gastric lumen-perfused rats for the study of acid secretion and in totally isolated vascularly perfused rat stomach preparations for the study of histamine release. The histamine in the effluent was determined by radioimmunoassay.
  
  
  RESULTS
  IL-8 (500 ng) significantly enhanced gastrin-stimulated acid secretion. IL-8, at a concentration of 500 ng/20 ml per 10 min, did not alter basal histamine release, but at 100 ng/20 ml and 500 ng/20 ml it dose-dependently increased gastrin-stimulated histamine release.
  
  
  CONCLUSIONS
  IL-8 enhances gastrin-stimulated acid secretion and histamine release from the rat stomach, which may explain the enhancing effect of IL-8 on gastric acid secretion.
authors.affiliation: The First Department of Internal Medicine, Saitama Medical Center, Saitama Medical School, 1981 Kamoda Tsujido-machi, Kawagoe 350-8550, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yakabi
authors.forename: Shoki
authors.initials: S
authors.lastname: Ro
authors.forename: Seiichi
authors.initials: S
authors.lastname: Miura
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Shino
authors.initials: S
authors.lastname: Ohno
authors.forename: Junichi
authors.initials: J
authors.lastname: Kawashima
authors.forename: Susumu
authors.initials: S
authors.lastname: Kurosawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D005755:Gastrins / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects / Q000502:physiology*; D016209:Interleukin-8 / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005755:Gastrins; D016209:Interleukin-8