Effect of cryoprotectants on the reconstitution of surfactant-free nanoparticles of poly(DL-lactide-co-glycolide).
Various cryoprotectants were tested to reconstitute the surfactant-free nanoparticles of poly(DL-lactide-co-glycolide) (PLGA). When 2.0% (w/v) of sucrose, trehalose and lactose were used, nanoparticles were completely reconstituted into aqueous solution and particle size was not significantly changed. Above 1.0% (w/v) of sucrose, trehalose and lactose, nanoparticles are well reconstituted whereas it was precipitated with 1.0% (w/v) of mannitol. Drug-encapsulated surfactant-free nanoparticles were quite reconstituted when 2.0% (w/v) of sucrose, trehalose and lactose. Drug release kinetics of nanoparticles was not significantly changed by cryoprotectants.
Jeong
Y-I
YI
Faculty of Applied Chemical Engineering, Chonnam National University, Gwangju, Korea. jeongyi@hanmail.net
Shim
Y-H
YH
Kim
C
C
Lim
G-T
GT
Choi
K-C
KC
Yoon
C
C
D003451:Cryoprotective Agents / Q000494:pharmacology*; D004187:Disaccharides / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D004355:Drug Stability; D005612:Freeze Drying / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry*; D049329:Nanostructures; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D012996:Solutions; D013501:Surface-Active Agents; D013696:Temperature
D003451:Cryoprotective Agents; D004187:Disaccharides; D004337:Drug Carriers; D011108:Polymers; D012996:Solutions; D013501:Surface-Active Agents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid