In vitro expression of natriuretic peptides in cardiomyocytes differentiated from monkey embryonic stem cells.
Functional characterization of ES cell-derived cardiomyocytes is important for differentiation control and application to the cell therapy. One of the crucial functions of cardiomyocytes is a production of atrial and brain natriuretic peptides (ANP and BNP, respectively), which have important endocrine, autocrine, and paracrine functions. In this study, we focused on the functional aspect of the cardiomyocytes differentiated from monkey ES cells in vitro and investigated the expression of ANP and BNP. Spontaneously contracting cells showed nodal-like action potentials, and expression of ANP and BNP by RT-PCR and immunocytochemistry. Interestingly, ANP and BNP expressions were detected as immunoreactive granules in the perinuclear area and these signals appeared to co-localize with trans-Golgi network. These findings suggest that monkey ES cells were able to differentiate into cardiomyocytes with functional characteristics in vitro and therefore can be used as a useful model to study mechanisms and functions in early cardiogenesis.
Abdelalim
Essam Mohamed
EM
Research Center For Animal Life Science, Shiga University of Medical Science, Tsukinowa-Cho, Seta, Otsu, Shiga 520-2192, Japan.
Takada
Tatsuyuki
T
Toyoda
Futoshi
F
Omatsu-Kanbe
Mariko
M
Matsuura
Hiroshi
H
Tooyama
Ikuo
I
Torii
Ryuzo
R
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D004594:Electrophysiology; D004622:Embryo, Mammalian / Q000166:cytology*; D000882:Haplorhini; D007150:Immunohistochemistry; D009200:Myocardial Contraction / Q000502:physiology; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D045265:Natriuretic Peptides / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*
D045265:Natriuretic Peptides