title: The evolutionary relationship of the domain architectures in the RhoGEF-containing proteins.
abstract: Domain insertions and deletions lead to variations in the domain architectures of the proteins from their common ancestor. In this work, we investigated four groups of the RhoGEF-containing proteins from different organisms with domain architectures RhoGEF-PH-SH3, SH3-RhoGEF-PH, RhoGEF-PH, and SH3-RhoGEF defined in the Pfam database. The phylogenetic trees were constructed using each individual domain and/or the combinations of all the domains. The phylogenetic analysis suggests that RhoGEF-PH-SH3 and SH3-RhoGEF-PH might have evolved from RhoGEF-PH through the insertion of SH3 independently, while SH3-RhoGEF of proteins in fruit fly might have evolved from SH3-RhoGEF-PH by the degeneration of PH domain.
authors.affiliation: The Key Laboratory of the Ministry of Education for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, China.
authors.forename: Qing Lan
authors.initials: QL
authors.lastname: Sun
authors.forename: Hong Jun
authors.initials: HJ
authors.lastname: Zhou
authors.forename: Kui
authors.initials: K
authors.lastname: Lin
mesh_terms: D000818:Animals; D019143:Evolution, Molecular; D020662:Guanine Nucleotide Exchange Factors / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D064067:Rho Guanine Nucleotide Exchange Factors
chemical_list: D020662:Guanine Nucleotide Exchange Factors; D064067:Rho Guanine Nucleotide Exchange Factors