title: Safe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity.
abstract: Concern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist.
authors.affiliation: DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa. rw1@sun.ac.za
authors.forename: Robin
authors.initials: R
authors.lastname: Warren
authors.forename: Marianna
authors.initials: M
authors.lastname: de Kock
authors.forename: Erica
authors.initials: E
authors.lastname: Engelke
authors.forename: Roseline
authors.initials: R
authors.lastname: Myburgh
authors.forename: Nico
authors.initials: N
authors.lastname: Gey van Pittius
authors.forename: Thomas
authors.initials: T
authors.lastname: Victor
authors.forename: Paul
authors.initials: P
authors.lastname: van Helden
mesh_terms: D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000097:blood / Q000302:isolation & purification*; D006358:Hot Temperature; D006801:Humans; D007757:Laboratory Infection / Q000517:prevention & control*; D009164:Mycobacterium Infections / Q000175:diagnosis; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D017751:Safety Management / Q000379:methods
chemical_list: D004269:DNA, Bacterial