Cardiac development in crayfish: ontogeny of cardiac physiology and aerobic metabolism in the red swamp crayfish Procambarus clarkii.
The cardiovascular system performs key physiological functions even as it develops and grows. The ontogeny of cardiac physiology was studied throughout embryonic and larval development in the red swamp crayfish Procambarus clarkii using videomicroscopic dimensional analysis. The heart begins to contract by day 13 of development (at 25 degrees C, 20 kPa O2). Prior to eclosion, heart rate (fH) decreases significantly. Previous data suggests that the decrease in cardiac parameters prior to hatching may be due to an oxygen limitation of the embryo. Throughout development, metabolizing mass and embryonic oxygen consumption primarily increased while egg surface area remains constant. The limited area for gas exchange of the egg membrane, in combination with the increasing oxygen demand of the embryo could result in an inadequate diffusive supply of oxygen to developing tissues. To determine if the decrease in cardiac function was the result of an internal hypoxia experienced during late embryonic development, early and late stage embryos were exposed to hyperoxic water (PO2 = 40 kPa O2). The fH in late stage embryos increased significantly over control values when exposed to hyperoxic water suggesting that the suppression in cardiac function observed in late stage embryos is likely due to a limited oxygen supply.
Harper
S L
SL
Department of Environmental and Molecular Toxicology, Oregon State University, 1011 ALS, Corvallis, OR 97331, USA. harpers@science.oregonstate.edu
Reiber
C L
CL
D000332:Aerobiosis / Q000502:physiology; D000818:Animals; D003400:Astacoidea / Q000196:embryology* / Q000502:physiology*; D002302:Cardiac Output / Q000502:physiology; D015687:Cell Hypoxia / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D006321:Heart / Q000196:embryology / Q000502:physiology; D006339:Heart Rate / Q000502:physiology; D008297:Male; D010101:Oxygen Consumption / Q000502:physiology; D013318:Stroke Volume / Q000502:physiology