Selective mineral elements concentration of the intestinal mucosa role of the lysosomes of duodenal enterocytes in the handling of mineral elements after intragastric administration.
Intragastric administration to rats of four soluble lanthanides cerium, lanthanum, europium, thulium and of three soluble elements of group IIIA aluminium, indium and gallium has been shown in previous studies. In this work two new rare earths gadolinium and terbium were studied using the same protocols and the same methods (transmission electron microscopy and ion microanalysis). among the previously studied elements, some of them were administered simultaneously on the one hand aluminium and indium, and on the other hand, lanthanum and cerium. These metals were looked for in intestinal mucosa, liver and kidney. The results showed: a) gadolinium and terbium were selectively concentrated in lysosomes of duodenal enterocytes, precipitated as non-soluble phosphate salts and eliminated with the cell's turn-over in less than 48 hr; b) Administered simultaneously, they precipitated in the same lysosome. c/ none of them was observed in the liver or kidney even with high dose. This study brings up to nine the number of elements forming a non-soluble phosphate salts, explaining their precipitation in lysosomes. None of them have a physiological role, two are toxic (aluminium and indium). This rapid intralysosomal concentration is an efficient mechanism which limits the diffusion of the foreign substances through the digestive barrier, then permits their elimination along with the cytoptose phenomenon in the intestinal lumen.
Tekaya
L
L
Laboratoire de Physiologie, Faculté de Médecine de Tunis 15, Rue Jebel Lakhdhar, 1007 Bab Sâadoun, Tunis, Tunisie. souhaila.aloui@auf.org
Ayadi
A
A
Fehri
E
E
El Hili
A
A
D000818:Animals; D001692:Biological Transport; D011232:Chemical Precipitation; D004386:Duodenum / Q000648:ultrastructure; D004577:Electron Probe Microanalysis; D020895:Enterocytes / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D007583:Jejunum / Q000648:ultrastructure; D028581:Lanthanoid Series Elements / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D008247:Lysosomes / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000648:ultrastructure; D008954:Models, Biological; D010588:Phagosomes / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D018629:Spectrometry, Mass, Secondary Ion
D028581:Lanthanoid Series Elements