Detection of benzopyrene-deoxyguanosine adducts by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
A method for the detection of BPDE-d guanosine adducts using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is described and illustrated. The results indicate that MALDI is capable of detecting two other DNA benzopyrene adducts, which are trace products formed during the synthesis of BPDE-d guanosine. This MALDI-TOFMS method offers the potential for the detection of DNA adducts in human tissue using very limited sample purification and preparation.
Qiao
Zhuanhong
Z
Department of Chemistry, Howard University, Washington, DC 20059, USA.
Udeochu
Uche
U
Jimerson
Toiya
T
Fletcher
Melissa
M
Bakare
Oladapo
O
Hosten
Charles M
CM
D001580:Benzopyrenes / Q000032:analysis* / Q000737:chemistry*; D018736:DNA Adducts / Q000032:analysis* / Q000737:chemistry*; D003849:Deoxyguanosine / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001580:Benzopyrenes; D018736:DNA Adducts; D003849:Deoxyguanosine