Coreceptor usage of primary HIV type 1 isolates obtained from different lymph node subsets.
Biological characteristics of virus quantitatively rescued from different cell types present in lymph nodes of HIV-1-infected individuals in various stages of their disease were determined, not including patients with AIDS defining illness. Viruses were obtained by cocultivation with donor monocyte-derived macrophages and T-lymphocytes and their biological phenotype compared to viruses obtained from the peripheral blood mononuclear cells of the same patient. The biological phenotype was determined on established cell lines (U937-2, CEM, and MT-2) and on the U87.CD4 coreceptor indicator cell lines and variable region 3 (V3) of the envelope was subjected to direct sequencing. All isolates obtained from lymph node subsets used CCR5 as coreceptor. Furthermore, these viruses were also sensitive to inhibition by beta-chemokines as analyzed for viruses of one patient. All 12 V3 regions showed a unique sequence indicating compartmentalization within each patient. The biological phenotype of CCR5-dependent (R5) HIV-1 isolates obtained from PBMC resembles the phenotype of viruses isolated from different lymph node cell subsets.
Casper
Charlotte
C
Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden.
Mild
Mattias
M
Jansson
Marianne
M
Karlsson
Anders
A
Holmberg
Viveca
V
Bratt
Göran
G
Van Paaschen
Hubertus
H
Biberfeld
Peter
P
Björndal
Asa
A
Albert
Jan
J
Popovic
Mikulas
M
Fenyö
Eva Maria
EM
D000595:Amino Acid Sequence; D019742:Chemokines, CC / Q000494:pharmacology; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000821:virology*; D008969:Molecular Sequence Data; D010641:Phenotype; D019713:Receptors, CCR5 / Q000378:metabolism*; D014779:Virus Replication
D019742:Chemokines, CC; D019713:Receptors, CCR5