Immunohistochemistry of adhesion molecules, metalloproteinases and NO-synthases in extravillous trophoblast of tubal pregnancy.
Trophoblast invasion in uterine pregnancy is fine-tuned for the remodelling of the uterine wall and its vascularization. Tubal pregnancy, which occurs in a limited number of patients, involves a dramatic trophoblast invasion in a context of a poor decidualization. By studying the histology of the extravillous trophoblast (EVC) in the anchoring villi, the Ki67 labelling, the location of several adhesion markers (cytokeratin-7, alpha1, alpha6, alphaV, beta1, beta4 integrin subunits and E-cadherin, V/E-cadherin), metalloproteinases (MMP-2, 9 and11), NOS2 and 3, we aimed to detect the specificity of tubal compared to intrauterine pregnancies. No difference could be observed between meso or anti-salpingial trophoblast proliferation or invasion using Ki67. Cytokeratin-7 allowed detection of spindle-shape EVCs and we identified some decidualized stromal cells. Integrins alpha1, beta1 and alphaV, and V/E-cadherin were expressed mainly in the distal EVC correspondingly to intrauterine pregnancy, with a poor expression of alpha1. Integrins alpha6 and beta4, E-cadherin were detected in the distal EVC in contrast to uterine pregnancy. MMP-2, 9, 11 were also shown in distal EVC. NOS2 and 3 labelled the perivascular EVC and NOS3 the endothelial cells of the tubal vessels. These changed distributions of adhesion molecules and MMP together with that of the basic and inducible NOS expressions could be related to mechanical effects in superficial implantation or to a failure of decidualization in tubal pregnancies.
Dubernard
G
G
Faculté de Médecine Saint Antoine, Physiopathologie de l'Implantation et du Développement-Université P. et M. Curie Upres 2396, 27 Rue de Chaligny, 75571 Paris Cedex 12, France.
Galtier-Fougairolles
M
M
Cortez
A
A
Uzan
S
S
Challier
J C
JC
D015415:Biomarkers; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D049109:Cell Proliferation; D010064:Embryo Implantation; D005187:Fallopian Tubes / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053552:Keratin-7 / Q000378:metabolism; D019394:Ki-67 Antigen; D045726:Metalloproteases / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D010641:Phenotype; D011247:Pregnancy; D011274:Pregnancy, Tubal / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D014327:Trophoblasts / Q000378:metabolism* / Q000473:pathology*
D015415:Biomarkers; D015815:Cell Adhesion Molecules; D053552:Keratin-7; D019394:Ki-67 Antigen; D019001:Nitric Oxide Synthase; D045726:Metalloproteases