title: Proteome analysis of up-regulated proteins in the rat spinal cord induced by transection injury.
abstract: The inability of the CNS to regenerate in adult mammals propels us to reveal associated proteins involved in the injured CNS. In this paper, either thoracic laminectomy (as sham control) or thoracic spinal cord transection was performed on male adult rats. Five days after surgery, the whole spinal cord tissue was dissected and fractionated into water-soluble (dissolved in Tris buffer) and water-insoluble (dissolved in a solution containing chaotropes and surfactants) portions for 2-DE. Protein identification was performed by MS and further confirmed by Western blot. As a result, over 30 protein spots in the injured spinal cord were shown to be up-regulated no less than 1.5-fold. These identified proteins possibly play various roles during the injury and repair process and may be functionally categorized as several different groups, such as stress-responsive and metabolic changes, lipid and protein degeneration, neural survival and regeneration. In particular, over-expression of 11-zinc finger protein and glypican may be responsible for the inhibition of axonal growth and regeneration. Moreover, three unknown proteins with novel sequences were found to be up-regulated by spinal cord injury. Further characterization of these molecules may help us come closer to understanding the mechanisms that underlie the inability of the adult CNS to regenerate.
authors.affiliation: Department of Neurobiology, Institute of Basic Medical Sciences, Beijing, PR China.
authors.forename: Qinxue
authors.initials: Q
authors.lastname: Ding
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wu
authors.forename: Yaojun
authors.initials: Y
authors.lastname: Guo
authors.forename: Congjian
authors.initials: C
authors.lastname: Zhao
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Jia
authors.forename: Fanwen
authors.initials: F
authors.lastname: Kong
authors.forename: Bingyao
authors.initials: B
authors.lastname: Chen
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wang
authors.forename: Shaoxiang
authors.initials: S
authors.lastname: Xiong
authors.forename: Haiping
authors.initials: H
authors.lastname: Que
authors.forename: Shuqian
authors.initials: S
authors.lastname: Jing
authors.forename: Shaojun
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D020543:Proteome; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013119:Spinal Cord Injuries / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D009419:Nerve Tissue Proteins; D020543:Proteome