Identification of LMW glutenin-like genes from Secale sylvestre host.
Three low-molecular-weight (LMW) glutenin-like genes (designated as Ssy1, Ssy2 and Ssy3) from Secale sylvestre Host were isolated and characterized. The three genes consist of a predicted highly conservative signal peptide with 20 amino acids, a short N-terminal region with 13 amino acids, a highly variable repetitive domain and a less variable C-terminal domain. The deduced amino acid sequences of the three genes were the LMW-m type due to a methionine residue at the N-terminus. The phylogenic analysis indicated that the prolamin genes could be perfectly clustered into five groups, including HMW-GS, LMW-GS, alpha/beta-, gamma- and omega-prolamin. The LMW glutenin-like genes of S. sylvestre were more orthologous with the LMW-GS genes of wheat and B hordein genes of barley, which also had been confirmed by the homology analysis with the LMW-GS of wheat at Glu-A3, Glu-B3 and Glu-D3 loci. These results indicated that a chromosome locus (designated as Glu-R3) might be located on the R genome of S. sylvestre with the functions similar to the Glu-3 locus in wheat and its related species.
Shang
H Y
HY
Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan 611830, China.
Wei
Y M
YM
Long
H
H
Yan
Z H
ZH
Zheng
Y L
YL
D000595:Amino Acid Sequence; D032461:Chromosomes, Plant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D005983:Glutens / Q000235:genetics; D010802:Phylogeny; D040641:Quantitative Trait Loci / Q000235:genetics*; D012434:Secale / Q000235:genetics*
D005983:Glutens; C037302:glutenin