[The reaction mechanism between cefoperazone and human serum albumin].
The reaction mechanism between cefoperazone and human serum albumin (HSA) and the affinity between cefoperazone and beta-lactamase were investigated by spectrometry and spectrofluorimetry. The interaction dissociation constants of human serum albumin and cefoperazone have been determined from a double reciprocal Lineweaver-Burk plot. The binding distance and the transfer efficiency between cefoperazone and HSA were also obtained according to the theory of Förster' non-radiation energy transfer. The result suggested that the main binding force between cefoperazone and HSA is electrostatic force interaction. The high beta-lactamase stability of cefoperazone may be correlative with its molecular structure. The antibiotic activity and valency connect with transfer efficiency and dissociation constant. The effect of cefoperazone on the conformation of HSA was also analyzed using synchronous fluorescence spectrometry.
Liu
Luo-sheng
LS
School of Chemistry and Chemical Engineering, Shandong University, Ji'nan 250100, China.
Zhang
Yu-yi
YY
Wang
Xing-po
XP
D000465:Algorithms; D001667:Binding, Competitive; D002438:Cefoperazone / Q000737:chemistry* / Q000378:metabolism; D004597:Electroshock; D004735:Energy Transfer; D005453:Fluorescence; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D015394:Molecular Structure; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D001618:beta-Lactamases / Q000737:chemistry / Q000378:metabolism
D012709:Serum Albumin; D002438:Cefoperazone; D001618:beta-Lactamases