T cell repertoire and Epstein-Barr virus-specific T cell response in chronic active Epstein-Barr virus infection: a case study.
In a patient with chronic active Epstein-Barr virus infection associated with vasculitis and fulminant CD4+ T cell lymphoproliferative disorder, we probed the peripheral blood mononuclear cells (PBMC) for the presence of an EBV-specific T cell repertoire and tested the possible relationship between the lymphocytic infiltrate and the EBV-specific T cell response. Our results give credence to the presence of an apparently normal EBV-specific memory T cell response after in vitro reactivation of the patient's PBMC with autologous infected B lymphoblastoid cell lines. In keeping with the characterization of the vasculitis, certain T cell subsets were detected after expansion of skin lesion-infiltrating lymphocytes and were found to be infected with EBV. These particular T cell expansions were neither the effectors nor the targets of the in vitro reactivated EBV-specific T cells, thus excluding a simple relationship between EBV, the skin lesions, and the T cell expansions frequently observed in these patients.
Gallot
Géraldine
G
INSERM U601,9 Quai Moncousu, 44093 Nantes cedex, France.
Hamidou
Mohamed A
MA
Clémenceau
Béatrice
B
Gaschet
Joëlle
J
Tiberghien
Pierre
P
Ferrand
Christophe
C
Vivien
Régine
R
Barbarot
Sébastien
S
Coste-Burel
Marianne
M
Moreau
Anne
A
Vié
Henri
H
D000328:Adult; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019556:COS Cells; D002460:Cell Line; D002469:Cell Separation; D002522:Chlorocebus aethiops; D002908:Chronic Disease; D018920:Coculture Techniques; D022801:Complementarity Determining Regions / Q000235:genetics; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000276:immunology*; D005805:Genes, MHC Class I / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008232:Lymphoproliferative Disorders / Q000209:etiology / Q000276:immunology; D008297:Male; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology; D012867:Skin / Q000276:immunology / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology / Q000821:virology; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology* / Q000821:virology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014657:Vasculitis / Q000209:etiology / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology
D022801:Complementarity Determining Regions; D004279:DNA, Viral; D018984:Epitopes, T-Lymphocyte; D010446:Peptide Fragments; D016693:Receptors, Antigen, T-Cell, alpha-beta; D014409:Tumor Necrosis Factor-alpha; D014764:Viral Proteins