title: Phylogenetic analysis of ORF5 and ORF7 sequences of porcine reproductive and respiratory syndrome virus (PRRSV) from PRRS-positive Italian farms: a showcase for PRRSV epidemiology and its consequences on farm management.
abstract: We investigated the dynamics of porcine reproductive and respiratory syndrome virus (PRRSV) variability in a range of swine PRRS-positive farms located in Northern Italy, to provide insights into the epidemiology and diffusion of the virus, particularly throughout the entire swine production chain. In this context, we also examined the effectiveness and the critical points of a recently developed gilts acclimatization program in swine breeder farms. To achieve these aims, we designed new primers and determined 64 complete open reading frame 5 (ORF5) sequences, representing Italian PRRSV field strains and the European vaccine Porcilis strain (Intervet); in addition, the more conserved ORF7 of 11 PRRSV strains were sequenced. The domains' prediction of their putative protein sequences was performed as well. Based on these sequences, phylogenetic trees were inferred which revealed a high degree of variability among the PRRSV Italian strains. The outcomes of the phylogenetic analysis showed that the most frequent source of infection in PRRS-positive farms (sow herds, nursery sites, fattening units) was the introduction of animals carrying a new variant and not the modification of already present variants; moreover, the integration of data from phylogenetic analysis and from the clinical and serological status of the swine herds suggested that the acclimatization program could be a valid tool to stabilize the PRRS clinical picture in farms, only when applied in combination with rigorous bio-security routine management and avoid the incoming of new PRRSV variants.
authors.affiliation: Laboratorio Tre Valli, Corte Pellegrina, San Martino Buon Albergo, 37036 Verona, Italy. patrizia.pesente@aia-spa.it
authors.forename: Patrizia
authors.initials: P
authors.lastname: Pesente
authors.forename: Valeria
authors.initials: V
authors.lastname: Rebonato
authors.forename: Gianpietro
authors.initials: G
authors.lastname: Sandri
authors.forename: Davide
authors.initials: D
authors.lastname: Giovanardi
authors.forename: Luigi Sperati
authors.initials: LS
authors.lastname: Ruffoni
authors.forename: Sandra
authors.initials: S
authors.lastname: Torriani
mesh_terms: D000595:Amino Acid Sequence; D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D001483:Base Sequence; D005260:Female; D007558:Italy / Q000453:epidemiology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D013552:Swine; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000032:analysis* / Q000145:classification / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; D014764:Viral Proteins; C114789:glycoprotein 5, PRRSV