[Stable transformation of Solanum rickii chloroplast DNA].
The biolistic method was used for genetic transformation of Solanum rickii chloroplasts with aadA gene encoding resistance to streptomycine and spectinomycine. Selective pressure was applied immediately after microbombardment to avoid appearance of mutant lines. The transplastomic Solanum rickii plants remained green during two years cultivation on the media supplemented with two antibiotics. There were no morphological differences between the transformed and the wild type plants.
Matveeva
N A
NA
Shakhovskiĭ
A M
AM
Kuchuk
N V
NV
D019470:Biolistics / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D018742:DNA, Chloroplast / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D005500:Follow-Up Studies; D017343:Genes, Plant; D005822:Genetic Vectors; D010788:Photosynthesis; D030821:Plants, Genetically Modified; D018087:Plastids; D012641:Selection, Genetic; D032322:Solanum / Q000235:genetics* / Q000254:growth & development; D013997:Time Factors; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic; D019076:Transgenes
D003470:Culture Media; D018742:DNA, Chloroplast; D018744:DNA, Plant