CKbeta8-1 alters expression of cyclin E in colony forming units-granulocyte macrophage (CFU-GM) lineage from human cord blood CD34+ cells.
A C6 beta-chemokine, CKbeta8-1, suppressed the colony formation of CD34+ cells of human cord blood (CB). Molecular mechanisms involved in CKbeta8-1-medicated suppression of colony formation of CD34+ cells are not known. To address this issue, the level of various G1/S cell cycle regulating proteins in CKbeta8-1-treated CD34+ cells were compared with those in untreated CD34+ cells. CKbeta8-1 did not significantly alter the expression of the G1/S cycle regulation proteins (cyclin D1, D3, and E), CDK inhibitor (p27and Rb), and other cell proliferation regulation protein (p53) in CB CD34+ cells. Here we describe an in vitro system in which CB CD34+ cells were committed to a multipotent progenitor lineage of colony forming units-granulocyte/macrophage (CFU-GM) by a simple combination of recombinant human (rh) GM-CSF and rhIL-3. In this culture system, we found that cyclin E protein appeared later and disappeared faster in the CKbeta8-1-treated cells than in the control cells during CFU-GM lineage development. These findings suggested that cyclin E may play a role in suppressing the colony formation of CFU-GM by CKbeta8-1.
Noh
Eui Kyu
EK
Immunomodulation Research Center, Department of Biological Science, University of Ulsan, Ulsan 680-749, Korea.
Ra
Jae Sun
JS
Lee
Seong Ae
SA
Kwon
Byoung S
BS
Han
In Seob
IS
D018952:Antigens, CD34 / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D019070:Cell Lineage; D002478:Cells, Cultured; D019742:Chemokines, CC / Q000494:pharmacology*; D019927:Cyclin E / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology*; D016193:G1 Phase / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D018952:Antigens, CD34; C105504:CCL23 protein, human; D018797:Cell Cycle Proteins; D019742:Chemokines, CC; D019927:Cyclin E; D006133:Growth Substances