title: Adipokine expression profile in adipocytes of different mouse models of obesity.
abstract: Adipose tissue produces and secretes multiple adipokines. Most studies on adipokine production/expression have been performed on whole adipose tissue. In addition, data concerning an overall of adipokine expression are scarce and can be heterogeneous depending on the obesity model studied. Our first aim was to compare the expression of adipokines involved in the interplay between obesity and insulin resistance in isolated adipocytes from different mouse models of obesity displaying different levels of weight gain and insulin sensitivity. The second aim was to determine perigonadal/subcutaneous ratio of each adipokine. Only resistin expression was decreased in obese mice without modifications in glucose and insulin blood levels. In addition to decreased levels of resistin, obesity models associated with hyperglycemia and hyperinsulinemia presented an increased expression of leptin and tumor necrosis factor-alpha (TNFalpha). Obese and diabetic mice were the only animals to exhibit high expression of plasminogen activator inhibitor type-1 and interleukin-6. All adipokines except TNFalpha were more heavily expressed in perigonadal than in subcutaneous adipocytes. Interestingly, fat-enriched diet and overweight on their own did not modify the distribution of adipokines between the two fat depots. However, severe obesity modified the distribution of proinflammatory adipokines. In conclusion, the level and number of adipokines with altered expression increased with obesity and hyperinsulinemia in mice. The physiopathological impact of depot-specific differences of adipokine expression in adipocytes remains to be clarified.
authors.affiliation: INSERM U586, Unité de recherches sur les obésités, Toulouse F-31432, France.
authors.forename: J
authors.initials: J
authors.lastname: Boucher
authors.forename: I
authors.initials: I
authors.lastname: Castan-Laurell
authors.forename: D
authors.initials: D
authors.lastname: Daviaud
authors.forename: C
authors.initials: C
authors.lastname: Guigné
authors.forename: M
authors.initials: M
authors.lastname: Buléon
authors.forename: C
authors.initials: C
authors.lastname: Carpéné
authors.forename: J S
authors.initials: JS
authors.lastname: Saulnier-Blache
authors.forename: P
authors.initials: P
authors.lastname: Valet
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D007333:Insulin Resistance / Q000502:physiology; D050152:Intra-Abdominal Fat / Q000166:cytology / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009765:Obesity / Q000378:metabolism*; D036361:Peptide Hormones / Q000378:metabolism*; D052243:Resistin / Q000378:metabolism; D013045:Species Specificity; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D020738:Leptin; D036361:Peptide Hormones; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha