Evaluation of the assurance GDS for E. coli O157:H7 method and assurance GDS for shigatoxin genes method in selected foods: collaborative study.
A multilaboratory collaborative study was conducted to compare the Assurance GDS for E. coli 0157:H7 method and the reference culture methods for the detection of E. coli 0157:H7 in orange juice, raw ground beef, and fresh lettuce. A separate companion assay, the Assurance GDS for Shigatoxin Genes method was also evaluated with the same test portions. Fifteen laboratories participated in the study. A Chi square analysis of each of the 3 food types at the high, low, and uninoculated control levels was performed. For all foods, the Assurance GDS for E. coli O157:H7 method and the Assurance GDS for Shigatoxin Genes method were equivalent to or better than the reference methods.
Feldsine
Philip T
PT
BioControl Systems, Inc., 12822 SE 32nd St, Bellevue, WA 98005, USA. ptf@biocontrolsys.com
Green
Shannon T
ST
Lienau
Andrew H
AH
Stephens
James
J
Jucker
Markus T
MT
Kerr
David E
DE
D000818:Animals; D001628:Beverages / Q000032:analysis; D002957:Citrus; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005504:Food Analysis / Q000379:methods*; D005516:Food Microbiology; D018545:Lactuca; D008460:Meat / Q000032:analysis; D022621:Shiga Toxin / Q000032:analysis* / Q000235:genetics
D022621:Shiga Toxin