CD24 and myosin light polypeptide 2 are involved in prevention of experimental autoimmune encephalomyelitis by myelin basic protein-pulsed dendritic cells.
We previously demonstrated that injection of myelin basic protein-pulsed (MBP-pulsed)--but not of unpulsed--autologous bone marrow-derived dendritic cells (DC) efficiently prevents experimental autoimmune encephalomyelitis (EAE) in Lewis rats. To define the molecules involved, we used 3 groups of rats pretreated subcutaneously with MBP-DC, or unpulsed DC, or PBS (control EAE). Four weeks later, all rats were immunized with encephalitogenic MBP peptide and adjuvant. Microarray analyses were done to screen for genes that differ among the 3 groups. Based on microarray analysis data, we used real-time PCR to measure expression of six probably involved genes in draining lymph node cells obtained on day 0, day 7 and day 14 post immunization (p.i.). Two of these 6 genes were consistently altered in both microarray analyses and RT-PCR. They are CD24 antigen being persistently low, and myosin light polypeptide 2 (Myl2) being high in the acute immune response in MBP-DC pretreated rats that develop resistance to EAE. These two genes could be targeted to treat EAE and, possibly, multiple sclerosis.
Liu
Xuan
X
Division of Neuroimmunology, Neurotec Department, Karolinska Institute, 14183 Stockholm, Sweden.
Lindberg
Raija
R
Xiao
Bao-Guo
BG
Steffensen
Knut R
KR
Leppert
David
D
Link
Hans
H
Huang
Yu-Min
YM
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D051927:CD24 Antigen / Q000378:metabolism*; D024722:Cardiac Myosins / Q000378:metabolism*; D003713:Dendritic Cells / Q000502:physiology*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000517:prevention & control*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D004676:Myelin Basic Protein / Q000008:administration & dosage* / Q000737:chemistry; D018994:Myosin Light Chains / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010455:Peptides; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
D051927:CD24 Antigen; D004676:Myelin Basic Protein; D018994:Myosin Light Chains; D010455:Peptides; D012333:RNA, Messenger; C107184:myosin light chain 2; D024722:Cardiac Myosins