Role of poly (ADP-ribose) polymerase in kidney transplant and its relationship with delayed renal function: multivariate analysis.
Kidney allografts undergo pretransplant cold ischemia and consequent ischemia-reperfusion injury (IR). Poly (ADP-Ribose) polymerase (PARP-1) overactivation leads to massive NAD+ consumption and ATP depletion with induction of cellular necrosis under ischemic conditions, which may lead to an increase in acute tubular necrosis (ATN) and a delay in total recovery of renal function (RFR) of the transplanted organ.


METHODS
Nuclear PARP-1 immunohistochemical expression (clone: PARP01) was studied in 155 paraffin-embedded renal biopsies from suboptimal donors and 95 kidney allograft biopsies with histopathological diagnosis of ATN.


RESULTS
In 50% of ATN biopsies, more than 50% of tubular nuclei were immunostained for PARP-1. PARP-1 expression was higher in ATN biopsies than in those from suboptimal donors (2.40 +/- 0.74 vs 0.92 +/- 1.13, P = 0.0001 Mann-Whitney). PARP-1 showed a statistically significant relationship with the time required to achieve effective diuresis (Rho:0.779), with serum creatinine, and with duration of cold ischemia (Rho:0.803). These relationships were stronger in the biopsies with ATN. In conclusion, multivariate analysis demonstrated that PARP-1 expression and cold ischemia duration in kidney biopsies with ATN predicted the short-term delay in total recovery of renal function and serum creatinine in the first month.
O'Valle
F
F
Department of Pathology, San Cecilio University Hospital and School of Medicine, University of Granada, Spain. fovalle@ugr.es
Benítez
M C
MC
Gómez-Morales
M
M
Bravo
J
J
Osuna
A
A
Martin-Oliva
D
D
Oliver
F J
FJ
Del Moral
R G
RG
D000328:Adult; D000368:Aged; D001706:Biopsy; D006085:Graft Survival; D006801:Humans; D007150:Immunohistochemistry; D007511:Ischemia; D016030:Kidney Transplantation / Q000473:pathology / Q000502:physiology*; D008875:Middle Aged; D015999:Multivariate Analysis; D009926:Organ Preservation; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D012079:Renal Circulation; D013030:Spain; D016896:Treatment Outcome
C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases