Effects of alpha tocopherol and verapamil on liver and small bowel following mesenteric ischemia-reperfusion.
OBJECTIVE
We have previously shown that alpha tocopherol is a potent antioxidant which prevents reperfusion injury in a kidney and small bowel autotransplant model. In this study, the effect of systemic alpha tocopherol and verapamil on small bowel and hepatic functions following mesenteric ischemi-reperfusion was evaluated.


METHODS
Fourty male Wistar Albino rats (weight, 250-300 g) all subjected to an ischemia-reperfusion experiment were divided into four groups of 10 as follows: Group 1: (SHAM), Group 2, given prophylactic and systemic alpha tocopherol, Group 3: given verapamil and Group 4: given (both verapamil and alpha tocopherol).


RESULTS
Glutaminase activities 120 minutes after reperfusion were found to be significant in liver tissues (p=0.004). The highest to the lowest glutaminase activities in liver tissue at 120 minutes after reperfusion were in Group 1, Group 3, Group 2 and Group 4 respectively. Significant differences in MDA levels were found in the small bowel at 30 minutes and 120 minutes time points (p<0.05). There were statistically significant higher glutaminase levels at 30 minutes and 120 minutes of reperfusion in the small bowel, especially in Group 4 (p=0.005).


CONCLUSIONS
Both small bowel and liver injuries reperfusion, can be decreased by prophylactic use of alpha tocopherol and verapamil. Glutaminase activity in liver tissue can also be affected by small bowel ischemia-reperfusion.
Yağmurdur
Mahmut Can
MC
Hacettepe Univertsity Faculty of Medicine, Department of General Surgery, Ankara. mcyagmurdur@hotmail.com
Ozdemir
Arif
A
Topaloğlu
Serdar
S
Kilinç
Kamer
K
Ozenç
Ahmet
A