[Thermochemical studies on the binding reaction of perseleno diphenyl 2,2'-diformic acid with bovine serum albumin].
The binding of newly compounded perseleno diphenyl 2,2'-diformic acid to bovine serum albumin(BSA) was studied at different temperatures using fluorescence spectrum and UV spectrum. The fluorescence quenching data was analyzed according to Stern-Volmer equation and Lineweaver-Burk double-reciprocal equation. It was showed that this quenching complies better with the charactristic of static fluorescence quenching. The binding constant, thermodynamic parameters, and the binding spot of the compound with certain structure, coming from perseleno diphenyl 2,2'-diformic acid and bovine serum albumin, were obtained. Besides, themechanism of static fluorescence quenching and the quality of binding power were both discussed. The information of the binding mode, the mechanism of its transportation, and some medical theories in human body were offered.
Yan
Cheng-nong
CN
College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434020, China.
Shangguan
Yun-feng
YF
Liu
Yi
Y
Hou
An-xin
AX
Qu
Song-sheng
SS
D000465:Algorithms; D000818:Animals; D002417:Cattle; D005453:Fluorescence; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D016566:Organoselenium Compounds / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012643:Selenium / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013816:Thermodynamics
D009942:Organometallic Compounds; D016566:Organoselenium Compounds; C550017:perselenodiphenyl 2,2'-diformic acid; D012710:Serum Albumin, Bovine; D012643:Selenium