Gene transfer mediated by sphingosine/ dioleoylphosphatidylethanolamine liposomes in the presence of poloxamer 188.
A cationic liposome system consisting of sphingosine (SP) and dioleoylphosphatidylethanolamine (DOPE) was developed for in vitro and in vivo gene transfer. A nonionic surface active agent of poloxamer 188 was incorporated in the formulations to stabilize the DNA/liposome complex. Comparison of the results obtained from systems with and without the effect of poloxamer 188 was made to investigate the efficiency of gene expression. In vitro transfection study of the DNA/liposome complex showed that with the effect of poloxamer 188, gene transfer into some cell lines was enhanced. In vivo systemic delivery of the DNA/liposome complex with poloxamer 188 demonstrated gene expression with improved luciferase activity in all major organs including lung, spleen, heart, liver, and kidney. High level transgene activity was found in lung and spleen with prolonged gene expression. This was attributed to poloxamer 188 that stabilized the liposome system and produced homogeneous DNA/liposome complex for enhancement of gene delivery.
Law
Sai-Lung
SL
Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan 11217, ROC. sllaw@vghtpe.gov.tw
Chuang
Tzu-Chao
TC
Kao
Ming-Ching
MC
Lin
Yen-Shing
YS
Huang
Kuo-Jung
KJ
D000818:Animals; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004339:Drug Compounding; D004563:Electrochemistry; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008156:Luciferases / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D020442:Poloxamer; D013110:Sphingosine / Q000737:chemistry*; D014018:Tissue Distribution
D008081:Liposomes; D010714:Phosphatidylethanolamines; D020442:Poloxamer; C094877:dioleoyl phosphatidylethanolamine; D004247:DNA; D008156:Luciferases; D013110:Sphingosine