title: Rapid identification of Ochroconis gallopava by a loop-mediated isothermal amplification (LAMP) method.
abstract: Ochroconis gallopava is a species of dematiaceous fungi recognized as a causative agent of zoonotic and emerging fungal infections. It affects the central nervous system and respiratory tracts of humans, birds and cats. We designed O. gallopava species-specific primer sets to aid in its identification by a loop-mediated isothermal amplification (LAMP) method based on the D1/D2 domain of the LSU rDNA sequence. The LAMP method successfully detected the gene from both fungal DNA and experimentally infected brains and spleens of mice and will be helpful in the diagnosis of O. gallopava infection.
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chiba 260-8673, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ohori
authors.forename: Shigeo
authors.initials: S
authors.lastname: Endo
authors.forename: Ayako
authors.initials: A
authors.lastname: Sano
authors.forename: Koji
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Yarita
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kamei
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyaji
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nishimura
mesh_terms: D000818:Animals; D003883:Arthrodermataceae / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D004271:DNA, Fungal / Q000032:analysis*; D003881:Dermatomycoses / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D051379:Mice; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D004271:DNA, Fungal