title: Optimized and validated initial-rate method for the determination of perindopril erbumine in tablets.
abstract: A simple and sensitive kinetic spectrophotometric method for the determination of perindopril in pharmaceutical preparations is described. The method is based on the interaction of drug with 1-chloro-2,4-dinitrobenzene (CDNB) in dimethylsulfoxide (DMSO) at 40+/-1 degrees C. The reaction is followed spectrophotometrically by measuring the rate of change of the absorbance at 420 nm. Under the optimized experimental conditions, the calibration curve showed a linear relationship over the concentration range of 20-140 microg/ml. The activation parameter such as E(a), deltaH*, deltaS* and deltaG* for this reaction were calculated and found to be 27.31 kJ/mol, 24.69 kJ/mol, -138.84 J/K/mol and 61.50 kJ/mol, respectively. The method has been successfully applied to the determination of perindopril in commercial dosage forms. Statistical comparison of the results with the Abdellatef's spectrophotometric method shows excellent agreement and indicates no significant difference between the methods compared in terms of accuracy and precision.
authors.affiliation: Analytical Research Division, Department of Chemistry, Aligarh Muslim University, India. cht17nr_amu@yahoo.com
authors.forename: Nafisur
authors.initials: N
authors.lastname: Rahman
authors.forename: Nishat
authors.initials: N
authors.lastname: Anwar
authors.forename: Mohammad
authors.initials: M
authors.lastname: Kashif
mesh_terms: D000465:Algorithms; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000008:administration & dosage / Q000032:analysis*; D002626:Chemistry, Pharmaceutical; D004121:Dimethyl Sulfoxide; D004137:Dinitrochlorobenzene; D007202:Indicators and Reagents; D020913:Perindopril / Q000008:administration & dosage / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013607:Tablets; D013816:Thermodynamics
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D004137:Dinitrochlorobenzene; D007202:Indicators and Reagents; D013607:Tablets; D020913:Perindopril; D004121:Dimethyl Sulfoxide