title: Monoamine oxidase inhibitory constituents from the fruits of Cudrania tricuspidata.
abstract: A methylene chloride soluble fraction of the fruits of Cudrania tricuspidata significantly inhibited the mouse brain monoamine oxidase (MAO). Three known prenylated isoflavones were isolated and identified by activity-guided fractionation. Gancaonin A (1), 4'-O-methylalpinumisoflavone (2), and alpinumisoflavone (3) inhibited MAO activity in a concentration-dependent manner with IC50 values of 19.4, 23.9, and 25.8 microM, respectively. Of these, gancaonin A (1) showed a selective and potent inhibitory effect against MAO-B (IC50 0.8 microM) than MAO-A (IC50 >800 microM). The kinetic analysis using Lineweaver-Burk plots indicated that gancaonin A (1) competitively inhibited MAO-B.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju Korea.
authors.forename: Xiang Hua
authors.initials: XH
authors.lastname: Han
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Hong
authors.forename: Ji Sang
authors.initials: JS
authors.lastname: Hwang
authors.forename: Seon Hwa
authors.initials: SH
authors.lastname: Jeong
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Hwang
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Myung Koo
authors.initials: MK
authors.lastname: Lee
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.forename: Jai Seup
authors.initials: JS
authors.lastname: Ro
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007700:Kinetics; D031624:Maclura; D008752:Methylene Chloride / Q000737:chemistry; D051379:Mice; D008995:Monoamine Oxidase / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: C507553:4'-O-methylalpinumisoflavone; D007529:Isoflavones; D008996:Monoamine Oxidase Inhibitors; D010936:Plant Extracts; C507552:gancaonin A; D008752:Methylene Chloride; C000154:alpinumisoflavone; D008995:Monoamine Oxidase