Naturally transformable Acinetobacter sp. strain ADP1 belongs to the newly described species Acinetobacter baylyi.
Genotypic and phenotypic analyses were carried out to clarify the taxonomic position of the naturally transformable Acinetobacter sp. strain ADP1. Transfer tDNA-PCR fingerprinting, 16S rRNA gene sequence analysis, and selective restriction fragment amplification (amplified fragment length polymorphism analysis) indicate that strain ADP1 and a second transformable strain, designated 93A2, are members of the newly described species Acinetobacter baylyi. Transformation assays demonstrate that the A. baylyi type strain B2(T) and two other originally identified members of the species (C5 and A7) also have the ability to undergo natural transformation at high frequencies, confirming that these five strains belong to a separate species of the genus Acinetobacter, characterized by the high transformability of its strains that have been cultured thus far.
Vaneechoutte
Mario
M
Department of Clinical Chemistry, Microbiology & Immunology, Ghent University Hospital, Blok A, De Pintelaan 185, 9000 Gent, Belgium. Mario.Vaneechoutte@UGent.be
Young
David M
DM
Ornston
L Nicholas
LN
De Baere
Thierry
T
Nemec
Alexandr
A
Van Der Reijden
Tanny
T
Carr
Emma
E
Tjernberg
Ingela
I
Dijkshoorn
Lenie
L
D000150:Acinetobacter / Q000145:classification* / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S