title: Effect of inoculum conditioning on hydrogen fermentation and pH effect on bacterial community relevant to hydrogen production.
abstract: The effect of conditioning for a variety of inoculums on fermentative hydrogen production was investigated. In addition, the effects of pH condition on hydrogen fermentation and bacterial community were investigated. The effect of conditioning on hydrogen production was different depending on the inoculum types. An appreciable hydrogen production was shown with anaerobic digested sludge and lake sediment without conditioning, however, no hydrogen was produced when refuse compost and kiwi grove soil were used as inoculums without conditioning. The highest hydrogen production was obtained with heat-conditioned anaerobic digested sludge, almost the same production was also obtained with unconditioned digested sludge. The pH condition considerably affected hydrogen fermentation, hydrogen gas was efficiently produced with unconditioned anaerobic sludge when the pH was controlled at 6.0 throughout the culture period and not when only the initial pH was adjusted to 6.0 and 7.0. Hydrogen production decreased when the culture pH was only adjusted at the beginning of each batch in continuous batch culture, and additionally, bacterial community varied with the change in hydrogen production. It was suggested that Clostridium and Coprothermobacter species played important role in hydrogen fermentation, and Lactobacillus species had an adverse effect on hydrogen production.
authors.affiliation: Department of Civil and Environmental Engineering, Kumamoto University, 2-39-1 Kurokami, Japan. goshi@kumamoto-u.ac.jp
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kawagoshi
authors.forename: Naoe
authors.initials: N
authors.lastname: Hino
authors.forename: Aya
authors.initials: A
authors.lastname: Fujimoto
authors.forename: Masaharu
authors.initials: M
authors.lastname: Nakao
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Fujita
authors.forename: Seiji
authors.initials: S
authors.lastname: Sugimura
authors.forename: Kenji
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D000693:Anaerobiosis / Q000502:physiology; D019149:Bioreactors / Q000382:microbiology*; D006094:Gram-Positive Bacteria / Q000254:growth & development*; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods
chemical_list: D012722:Sewage; D006859:Hydrogen