title: Serum paraoxonase 1 activity and lipid peroxidation levels in patients with age-related macular degeneration.
abstract: Our objective was to investigate antioxidant paraoxonase 1 (PON1) activity together with malondialdehyde (MDA) levels to evaluate oxidative stress in patients with age-related macular degeneration (AMD), an important cause of blindness in the elderly population. Serum PON1 activity and MDA levels were analyzed in 37 patients with AMD and compared with 29 healthy controls using a spectrophotometric method. Serum MDA levels were significantly higher in the patient group (2.76 +/- 1.28 nmol/ml) than controls (1.00 +/- 0.36 nmol/ml; p < 0.001), whereas PON1 activity was lower in the patient group (132.27 +/- 63.39 U/l) than controls (312.13 +/- 136.23 U/l; p < 0.001). There was a negative correlation between MDA and PON1 levels (r = -0.470, p < 0.001). We conclude that the observed increase in MDA levels may be related to decreased PON1 activity; the present data also demonstrated that an obvious negative correlation between PON1 activity and MDA levels exists in patients with AMD. PON1 is also an antioxidant agent, therefore effective antioxidant therapy to inhibit lipid peroxidation is necessary and agents to increase PON1 activity may be a therapeutic option in AMD.
authors.affiliation: Department of Biochemistry and Clinical Biochemistry, Medical Faculty, Erciyes University, Kayseri, Turkey. gbaskol@yahoo.com
authors.forename: Gulden
authors.initials: G
authors.lastname: Baskol
authors.forename: Sarper
authors.initials: S
authors.lastname: Karakucuk
authors.forename: Ayse Ozturk
authors.initials: AO
authors.lastname: Oner
authors.forename: Mevlut
authors.initials: M
authors.lastname: Baskol
authors.forename: Derya
authors.initials: D
authors.lastname: Kocer
authors.forename: Ertugrul
authors.initials: E
authors.lastname: Mirza
authors.forename: Recep
authors.initials: R
authors.lastname: Saraymen
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Ustdal
mesh_terms: D000368:Aged; D043303:Aryldialkylphosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008268:Macular Degeneration / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D013053:Spectrophotometry
chemical_list: D015415:Biomarkers; D043303:Aryldialkylphosphatase