PDSM, a motif for phosphorylation-dependent SUMO modification.
SUMO (small ubiquitin-like modifier) modification regulates many cellular processes, including transcription. Although sumoylation often occurs on specific lysines within the consensus tetrapeptide PsiKxE, other modifications, such as phosphorylation, may regulate the sumoylation of a substrate. We have discovered PDSM (phosphorylation-dependent sumoylation motif), composed of a SUMO consensus site and an adjacent proline-directed phosphorylation site (PsiKxExxSP). The highly conserved motif regulates phosphorylation-dependent sumoylation of multiple substrates, such as heat-shock factors (HSFs), GATA-1, and myocyte enhancer factor 2. In fact, the majority of the PDSM-containing proteins are transcriptional regulators. Within the HSF family, PDSM is conserved between two functionally distinct members, HSF1 and HSF4b, whose transactivation capacities are repressed through the phosphorylation-dependent sumoylation. As the first recurrent sumoylation determinant beyond the consensus tetrapeptide, the PDSM provides a valuable tool in predicting new SUMO substrates.
Hietakangas
Ville
V
Turku Centre for Biotechnology, University of Turku and Abo Akademi University, FI-20521, Turku, Finland.
Anckar
Julius
J
Blomster
Henri A
HA
Fujimoto
Mitsuaki
M
Palvimo
Jorma J
JJ
Nakai
Akira
A
Sistonen
Lea
L
D020816:Amino Acid Motifs / Q000235:genetics*; D015153:Blotting, Western; D016384:Consensus Sequence / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D047468:Immunoprecipitation; D008156:Luciferases; D010766:Phosphorylation; D050436:Regulatory Elements, Transcriptional / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*
D006360:Heat-Shock Proteins; D025841:Small Ubiquitin-Related Modifier Proteins; D008156:Luciferases