Identification of genes and proteins involved in the pleiotropic response to arsenic stress in Caenibacter arsenoxydans, a metalloresistant beta-proteobacterium with an unsequenced genome.
The effect of high concentrations of arsenic has been investigated in Caenibacter arsenoxydans, a beta-proteobacterium isolated from an arsenic contaminated environment and able to oxidize arsenite to arsenate. As the genome of this bacterium has not yet been sequenced, the use of a specific proteomic approach based on nano-high performance liquid chromatography tandem mass spectrometry (nanoLC-MS/MS) studies and de novo sequencing to perform cross-species protein identifications was necessary. In addition, a random mutational analysis was performed. Twenty-two proteins and 16 genes were shown to be differentially accumulated and expressed, respectively, in cells grown in the presence of arsenite. Two genes involved in arsenite oxidation and one in arsenite efflux as well as two proteins responsible for arsenate reduction were identified. Moreover, numerous genes and proteins belonging to various functional classes including information and regulation pathways, intermediary metabolism, cell envelope and cellular processes were also up- or down-regulated, which demonstrates that bacterial response to arsenic is pleiotropic.
Carapito
Christine
C
LSMBO, Laboratoire de Spectrométrie de Masse Bio-Organique ECPM, Bâtiment R5, 67087 Strasbourg, France CNRS-UMR 7509/Université Louis-Pasteur, Strasbourg, France.
Muller
Daniel
D
Turlin
Evelyne
E
Koechler
Sandrine
S
Danchin
Antoine
A
Van Dorsselaer
Alain
A
Leize-Wagner
Emmanuelle
E
Bertin
Philippe N
PN
Lett
Marie-Claire
MC
D000595:Amino Acid Sequence; D001151:Arsenic / Q000633:toxicity*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D020562:Betaproteobacteria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D008670:Metals / Q000494:pharmacology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger
D001426:Bacterial Proteins; D008670:Metals; D012333:RNA, Messenger; D001151:Arsenic