title: Microviscoelastic moduli of biomimetic cell envelopes.
abstract: Bioanalogue models of composite cell envelopes were designed by electrostatically driven self-assembly of actin shells inside giant vesicles. Viscoelastic relaxation moduli were measured between 0.03 and 20 s as a function of actin density by magnetic bead microrheometry. The shear relaxation spectra exhibited by the composite shells compare well with those of natural cell envelopes and bulk entangled actin networks. Absolute value of the shear modulus was measured for the first time by deformation field mapping. Shear and bending moduli agree well with values obtained by bead fluctuations analysis.
authors.affiliation: E22-Biophysik. Physik Department, Technical University of Munich, James Franck Strasse, D-85748 Garching, Germany. limozin@marseille.inserm.fr
authors.forename: Laurent
authors.initials: L
authors.lastname: Limozin
authors.forename: Alexander
authors.initials: A
authors.lastname: Roth
authors.forename: Erich
authors.initials: E
authors.lastname: Sackmann
mesh_terms: D000199:Actins / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry; D004548:Elasticity; D008051:Lipid Bilayers / Q000737:chemistry*; D008567:Membranes, Artificial; D014783:Viscosity
chemical_list: D000199:Actins; D008051:Lipid Bilayers; D008567:Membranes, Artificial