Genetic variability in the extracellular matrix protein as a determinant of risk for developing HTLV-I-associated neurological disease.
Aggrecan, which is a well-known proteoglycan in joint cartilage, also exists in the spinal cord and plays an important role in maintaining water content in the extracellular matrix structure. In this study, we first examined the variable number of tandem repeat (VNTR) polymorphism of the aggrecan gene in 227 HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients, in 217 HTLV-I-infected healthy carriers (HCs), and in 85 normal controls. The VNTR allele 28 (1,630 bp) was more frequently observed in HAM/TSP patients than in HCs (chi2=12.02, p=0.0005, odds ratio 1.79, 95% C.I. 1.29-2.50) and in controls (chi2=13.43, p=0.0002, odds ratio 2.54, 95% C.I. 1.52-4.25), although this allele was not related to disease progression or to HTLV-I provirus load. We also found that the aggrecan concentration in cerebrospinal fluid (CSF) from rapidly progressive HAM/TSP patients was significantly higher than in slowly progressive patients (corrected p=0.0145) but not in infected non-inflammatory neurological other disease controls (OND) (corrected p=0.078). We then analyzed this aggrecan VNTR polymorphism in the different set of patients with HAM/TSP (n=58) and healthy carriers (n=70). This analysis, again, revealed that allele 28 was detected more frequently in HAM/TSP group than in HCs (chi2=11.03, p=0.0009, odd ratio 3.04, 95% C.I. 1.55-5.97). The reproducibility of our study was regarded as a second- or third-class association by comparing combined p values and the Better Associations for Disease and GEnes (BADGE) system. Our results suggest that aggrecan polymorphism can be a novel genetic risk factor for developing HAM/TSP.
Nobuhara
Yasuyuki
Y
Department of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima, 890-8520, Japan.
Usuku
Koichiro
K
Saito
Mineki
M
Izumo
Shuji
S
Arimura
Kimiyoshi
K
Bangham
Charles R M
CR
Osame
Mitsuhiro
M
D053674:Aggrecans; D000483:Alleles; D001483:Base Sequence; D002353:Carrier State; D016022:Case-Control Studies; D011508:Chondroitin Sulfate Proteoglycans / Q000134:cerebrospinal fluid / Q000235:genetics* / Q000276:immunology; D004247:DNA / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000134:cerebrospinal fluid / Q000235:genetics* / Q000276:immunology; D005787:Gene Frequency; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics* / Q000276:immunology; D018598:Minisatellite Repeats; D015493:Paraparesis, Tropical Spastic / Q000134:cerebrospinal fluid / Q000209:etiology* / Q000235:genetics* / Q000276:immunology; D012307:Risk Factors
D053674:Aggrecans; D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D004247:DNA