title: Convenient treatment of acetonitrile-containing wastes using the tandem combination of nitrile hydratase and amidase-producing microorganisms.
abstract: This study aimed to construct an acetonitrile-containing waste treatment process by using nitrile-degrading microorganisms. To degrade high concentrations of acetonitrile, the microorganisms were newly acquired from soil and water samples. Although no nitrilase-producing microorganisms were found to be capable of degrading high concentrations of acetonitrile, the resting cells of Rhodococcus pyridinivorans S85-2 containing nitrile hydratase could degrade acetonitrile at concentrations as high as 6 M. In addition, an amidase-producing bacterium, Brevundimonas diminuta AM10-C-1, of which the resting cells degraded 6 M acetamide, was isolated. The combination of R. pyridinivorans S85-2 and B. diminuta AM10-C-1 was tested for the conversion of acetonitrile into acetic acid. The resting cells of B. diminuta AM10-C-1 were added after the first conversion involving R. pyridinivorans S85-2. Through this tandem process, 6 M acetonitrile was converted to acetic acid at a conversion rate of >90% in 10 h. This concise procedure will be suitable for practical use in the treatment of acetonitrile-containing wastes on-site.
authors.affiliation: Gifu Prefectural Institute for Bio-Industrial Technology, Kamihachiya, Minokamo, Gifu 505-0004, Japan. kouyama@bio.rd.pref.gifu.jp
authors.forename: Erina
authors.initials: E
authors.lastname: Kohyama
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yoshimura
authors.forename: Daisuke
authors.initials: D
authors.lastname: Aoshima
authors.forename: Toyokazu
authors.initials: T
authors.lastname: Yoshida
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Kawamoto
authors.forename: Toru
authors.initials: T
authors.lastname: Nagasawa
mesh_terms: D000097:Acetonitriles / Q000378:metabolism*; D000581:Amidohydrolases / Q000378:metabolism*; D001673:Biodegradation, Environmental; D042063:Caulobacteraceae / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D012336:RNA, Ribosomal, 16S; D012240:Rhodococcus / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D014866:Waste Products
chemical_list: D000097:Acetonitriles; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014866:Waste Products; D000581:Amidohydrolases; C019665:amidase; D006836:Hydro-Lyases; C048831:nitrile hydratase; C032159:acetonitrile