DNA hole transport on an electrode: application to effective photoelectrochemical SNP typing.
A useful feature of DNA is that long-range hole transport through DNA is readily achieved. Photostimulated long-range hole transport through DNA has prospective use in the development of a conceptually new electrochemical single-nucleotide polymorphism (SNP) typing method for use as a versatile platform for gene diagnostics and pharmacogenetics. We have applied artificial DNAs designed for photostimulated long-range hole transport through DNA to SNP typing. By hybridizing photosensitizer-equipped DNA probes, immobilized on gold working electrodes, with a target DNA strand containing an SNP site, we observed a cathodic photocurrent, which markedly changed depending on the nature of the base at the specific site. The use of a combination of hole-transporting bases constitutes a very powerful method for a single-step electrochemical assay applicable to SNP typing of all types of sequences.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan. okamoto@sbchem.kyoto-u.ac.jp
Kamei
Taku
T
Saito
Isao
I
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D004563:Electrochemistry; D004566:Electrodes; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D010777:Photochemistry; D020641:Polymorphism, Single Nucleotide
D015342:DNA Probes; D009841:Oligonucleotides; D004247:DNA