title: DNA-based gels for oral delivery of probiotic bacteria.
abstract: A single-stranded DNA, readily extracted from industrial discarded salmon milt, was used to prepare hydrogels and complex gels by cross-linking with gelatin and kappa-carrageenan, for the oral delivery of probiotic bacteria. The complex gels showed a higher protective capability over the hydrogels for approximately one log scale. However, the hydrogels were more stable during storage at 4 degrees C. The Lactobacillus and Lactococcus due to protection of the hydrogels could better tolerate to acid than the Bifidobacterium. Furthermore, food-graded hydrogels were prepared and optimized to a similar protective capability for future applications.
authors.affiliation: Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Benjamas
authors.initials: B
authors.lastname: Jonganurakkun
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Nodasaka
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sakairi
authors.forename: Norio
authors.initials: N
authors.lastname: Nishi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001644:Bifidobacterium; D002351:Carrageenan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D005780:Gelatin / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis*; D007778:Lactobacillus; D017045:Lactococcus; D008855:Microscopy, Electron, Scanning; D019936:Probiotics / Q000008:administration & dosage*; D012474:Salmon
chemical_list: D020100:Hydrogels; D002351:Carrageenan; D005780:Gelatin; D004247:DNA