DNA-based gels for oral delivery of probiotic bacteria.
A single-stranded DNA, readily extracted from industrial discarded salmon milt, was used to prepare hydrogels and complex gels by cross-linking with gelatin and kappa-carrageenan, for the oral delivery of probiotic bacteria. The complex gels showed a higher protective capability over the hydrogels for approximately one log scale. However, the hydrogels were more stable during storage at 4 degrees C. The Lactobacillus and Lactococcus due to protection of the hydrogels could better tolerate to acid than the Bifidobacterium. Furthermore, food-graded hydrogels were prepared and optimized to a similar protective capability for future applications.
Jonganurakkun
Benjamas
B
Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan.
Nodasaka
Yoshinobu
Y
Sakairi
Nobuo
N
Nishi
Norio
N
D000284:Administration, Oral; D000818:Animals; D001644:Bifidobacterium; D002351:Carrageenan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D005780:Gelatin / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis*; D007778:Lactobacillus; D017045:Lactococcus; D008855:Microscopy, Electron, Scanning; D019936:Probiotics / Q000008:administration & dosage*; D012474:Salmon
D020100:Hydrogels; D002351:Carrageenan; D005780:Gelatin; D004247:DNA