[AKAP82 expression in sperm in asthenospermia].
OBJECTIVE
To study the expression of AKAP82 which was one of A-kinase anchor proteins in sperm in asthenospermia.


METHODS
Sperm from 41 patients with asthenospermia (experimental group) and 27 healthy fertile men (control group). Motility parameters were estimated with a computer-assisted semen analysis (CASA) technique. Electrophoretic and immunoblot analysis and indirect immunofluorescence of sperm were assayed in experimental group and control group respectively.


RESULTS
Eleven cases in experimental group showed abnormal bands or deletion of normal band in immunoblot analysis, 2 of which were also abnormal in immunofluorescence results, and while only 2 abnormal in the control. A significant difference between experimental and control group was observed (P < 0.05).


CONCLUSIONS
Abnormality of AKAP82 expression in sperm may be one of the etiological factors in asthenospermia.
Li
Yang
Y
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. liyang7365@163.com
Liu
Ji-hong
JH
Wang
Tao
T
Ye
Zhang-qun
ZQ
D000328:Adult; D000818:Animals; D015153:Blotting, Western; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000378:metabolism*; D011498:Protein Precursors / Q000096:biosynthesis*; D011817:Rabbits; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*
D011498:Protein Precursors; C116030:pro-AKAP82 protein, mammalian