title: B-cell differentiation immunophenotypes in classical Hodgkin lymphomas.
abstract: The bcl6/CD10/MUM1/CD138 B-cell differentiation immunophenotypes were analysed in 101 cases of classical Hodgkin lymphomas (cHL) aiming to elucidate their histogenesis. Three major bcl6/CD10/MUM1/CD138 immunophenotypes were distinguished on the basis of the immunohistochemical positivity of Hodgkin and Reed-Sternberg (H/RS) cells: (a) the late germinal center (GC)/early post-GC B-cell-like immunophenotype (bcl6-/CD10-/MUM1+/CD138-); 59/101 cases (59%), (b) the post-GC B-cell-like immunophenotype (bcl6-/CD10-/MUM1+/CD138+); 24/101 cases (24%) and (c) the indeterminate immunophenotype (bcl6+/CD10-/MUM1+/CD138-: 14 cases and bcl6+/CD10-/MUM1+/CD138+: four cases); 18/101 cases (18%). The above findings indicate that H/RS cells in most cHL display bcl6/CD10/MUM1/CD138 immunophenotypes consistent with late GC/early post-GC or post-GC B-cell differentiation. In addition, H/RS cells in a small fraction of cHL display indeterminate bcl6/CD10/MUM1/CD138 immunophenotypic profiles which are characterized by simultaneous expression of GC, late GC/early post-GC and post-GC B-cell differentiation proteins. These immunophenotypic profiles do not correspond to the differentiation immunophenotypes of normal B-cells and their identification in a part of cHL suggests that the differentiation process of H/RS cells is not complete in a fraction of these cells and/or is still ongoing at the time of observation.
authors.affiliation: Department of Pathology, University of Ioannina, Ioannina, Greece. mbai@cc.uoi.gr
authors.forename: Maria
authors.initials: M
authors.lastname: Bai
authors.forename: Vassilios
authors.initials: V
authors.lastname: Panoulas
authors.forename: Alexandra
authors.initials: A
authors.lastname: Papoudou-Bai
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Horianopoulos
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Kitsoulis
authors.forename: Kalliopi
authors.initials: K
authors.lastname: Stefanaki
authors.forename: Dimitra
authors.initials: D
authors.lastname: Rontogianni
authors.forename: Niki John
authors.initials: NJ
authors.lastname: Agnantis
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Kanavaros
mesh_terms: D000943:Antigens, Differentiation / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D002454:Cell Differentiation / Q000276:immunology*; D018858:Germinal Center / Q000473:pathology; D006689:Hodgkin Disease / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D050835:Interferon Regulatory Factors / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D015260:Neprilysin / Q000096:biosynthesis; D010641:Phenotype; D011509:Proteoglycans / Q000096:biosynthesis; D051560:Proto-Oncogene Proteins c-bcl-6 / Q000096:biosynthesis; D053668:Syndecan-1; D053667:Syndecans; D000099218:Interferon Regulatory Factor-4
chemical_list: D000943:Antigens, Differentiation; D050835:Interferon Regulatory Factors; D008562:Membrane Glycoproteins; D015260:Neprilysin; D011509:Proteoglycans; D051560:Proto-Oncogene Proteins c-bcl-6; D053668:Syndecan-1; D053667:Syndecans; D000099218:Interferon Regulatory Factor-4; C508241:SDC1 protein, human