title: VraSR two-component regulatory system and its role in induction of pbp2 and vraSR expression by cell wall antimicrobials in Staphylococcus aureus.
abstract: The VraS/VraR two-component system (VraSR) regulates transcriptional induction of penicillin-binding protein 2 (encoded by pbp2) by vancomycin in Staphylococcus aureus. We have now defined the vraSR operon and determined that its induction by beta-lactams as well as by vancomycin is autoregulated. Induction of the pbp2 and vraSR operons by beta-lactams and related compounds within 1 hour after exposure to the antimicrobials was dependent on vraS. However, when a disk diffusion assay that can detect induction of genes over an extended time period was used, induction of the pbp2 operon was mediated by some beta-lactams, including oxacillin; this induction was independent of vraS.
authors.affiliation: Department of Pediatrics, The University of Chicago, 5841 S. Maryland Ave., MC 6054, Chicago, Illinois 60637, USA.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Yin
authors.forename: Robert S
authors.initials: RS
authors.lastname: Daum
authors.forename: Susan
authors.initials: S
authors.lastname: Boyle-Vavra
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry*; D004268:DNA-Binding Proteins; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D009876:Operon; D046915:Penicillin-Binding Proteins; D015398:Signal Transduction; D013211:Staphylococcus aureus / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D046915:Penicillin-Binding Proteins; C478170:VraR protein, Staphylococcus aureus; C478169:VraS protein, Staphylococcus aureus; D014640:Vancomycin