Probing the B-to-Z-DNA duplex transition using terminally stacking ethynyl pyrene-modified adenosine and uridine bases.
Pyrene-modified adenosine and uridine bases located in the dangling positions of G,C-alternating oligodeoxynucleotides undergo pi-stacking in their B-DNA duplexes, but not in their Z-DNA duplexes; fluorescence quenching in the former, through photoinduced electron transfer, but not in the latter, allows the state of the B-to-Z-DNA transition to be characterized visually.
Seo
Young Jun
YJ
National Research Laboratory, Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
Kim
Byeang Hyean
BH
D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D043542:DNA, Z-Form / Q000737:chemistry*; D004583:Electrons; D005453:Fluorescence; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010777:Photochemistry; D011721:Pyrenes / Q000737:chemistry*; D014529:Uridine / Q000031:analogs & derivatives / Q000737:chemistry*
D043542:DNA, Z-Form; D009838:Oligodeoxyribonucleotides; D011721:Pyrenes; D004247:DNA; D000241:Adenosine; D014529:Uridine