Ultrafast diagnosis of the genetic-related disorders using the combined technologies of multiplex PCR and multichannel microchip electrophoresis.
For the diagnosis of unexplained male infertility a multiplex PCR for 6 markers, which are well-known as candidate genes for studying male infertility and located on the human Y-chromosome, has been designed. The multiplex PCR products have been separated on a 12 channel microchip electrophoresis system, which can analyze different samples simultaneously. By combining the technologies of multiplex PCR with multichannel microchip electrophoresis, the number of the DNA markers that can be screened simultaneously is increased to be 72 marker (12 x 6) in a single run while the electrophoresis analysis time is reduced to be only 180 s.
Jabasini
Mohammad
M
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Ewis
Ashraf A
AA
Xu
Feng
F
Ping
Guichen
G
Fouad
Maged
M
Shinka
Toshikatsu
T
Nakahori
Yutaka
Y
Ishikawa
Mitsuru
M
Baba
Yoshinobu
Y
D046209:Electrophoresis, Microchip / Q000379:methods*; D030342:Genetic Diseases, Inborn / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity