title: Liposome-mediated transient transfection reduces cholesterol-dependent coxsackievirus infectivity.
abstract: Liposome-mediated gene delivery provides a powerful strategy for the study of gene function and for gene therapy. Coxsackievirus B3 is an important human pathogen associated with various diseases. Here we reported that liposome-mediated transient transfection of plasmid cDNA inhibited coxsackieviral replication at the levels of RNA, protein and viral progeny release. These inhibitory effects were observed in various cell types and by using different liposome reagents. We further showed that the inhibition was likely due to the lack of virus attachment. Moreover, we showed that addition of cholesterol restored, at least in part, the viral infectivity. Interestingly, we found that membrane cholesterol levels were unchanged during transfection, indicating that disruption rather than depletion of membrane cholesterol contributes to the inhibitory effects of transfection. Our data suggest that liposome-mediated cDNA transient transfection inhibits coxsackievirus infectivity via inhibition of viral attachment, which is likely occurring through the changes of membrane cholesterol integrity.
authors.affiliation: Department of Pathology and Laboratory Medicine, The James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research, University of British Columbia-St. Paul's Hospital, Vancouver, Canada.
authors.forename: Jerry
authors.initials: J
authors.lastname: Wong
authors.forename: Jingchun
authors.initials: J
authors.lastname: Zhang
authors.forename: Guang
authors.initials: G
authors.lastname: Gao
authors.forename: Mitra
authors.initials: M
authors.lastname: Esfandiarei
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Si
authors.forename: Yahong
authors.initials: Y
authors.lastname: Wang
authors.forename: Bobby
authors.initials: B
authors.lastname: Yanagawa
authors.forename: Agripina
authors.initials: A
authors.lastname: Suarez
authors.forename: Bruce
authors.initials: B
authors.lastname: McManus
authors.forename: Honglin
authors.initials: H
authors.lastname: Luo
mesh_terms: D015153:Blotting, Western; D002784:Cholesterol / Q000378:metabolism* / Q000494:pharmacology; D003384:Coxsackievirus Infections / Q000517:prevention & control*; D029822:Enterovirus B, Human / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology*; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008081:Liposomes; D011991:Receptors, Virus / Q000378:metabolism; D014162:Transfection / Q000379:methods*; D010948:Viral Plaque Assay
chemical_list: D008081:Liposomes; D011991:Receptors, Virus; C105941:coxsackievirus B receptor; D002784:Cholesterol