Comparative study of the CCF-like pattern recognition protein in different Lumbricid species.
Coelomic fluid of the Lumbricid Eisenia fetida contains a 42-kDa pattern recognition protein named coelomic cytolytic factor (CCF) that binds microbial cell wall components and triggers the activation of the prophenoloxidase cascade, an important invertebrate defense pathway. Here we report on the sequence characterization of CCF-like molecules of other Lumbricids: Aporrectodea caliginosa, Aporrectodea icterica, Aporrectodea longa, Aporrectodea rosea, Dendrobaena veneta, Lumbricus rubellus and Lumbricus terrestris, and show that CCF from E. fetida has a broader saccharide-binding specificity, being the only one recognizing N,N'-diacetylchitobiose. We suggest that the broad recognition repertoire of E. fetida CCF reflects a particular microbial environment this species lives in.
Silerová
Marcela
M
Department of Immunology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic.
Procházková
Petra
P
Josková
Radka
R
Josens
Guy
G
Beschin
Alain
A
De Baetselier
Patrick
P
Bilej
Martin
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004156:Catechol Oxidase / Q000378:metabolism; D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D004187:Disaccharides / Q000378:metabolism; D004792:Enzyme Precursors / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009835:Oligochaeta / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D016415:Sequence Alignment; D013379:Substrate Specificity
D003603:Cytotoxins; D004187:Disaccharides; D004792:Enzyme Precursors; D037102:Lectins; C010913:N,N-diacetylchitobiose; C038451:pro-phenoloxidase; D004156:Catechol Oxidase