[Identification of triacylglycerols in coix oil by high performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry].
OBJECTIVE
To identify triacylglycerols in coix oil.


METHODS
High performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry was used for identification. The experiment was operated under the conditions: spray voltage at 3 000 V, capillary temperature at 250 degrees C, APCI vaporizer temperature at 400 degrees C, and corona current of 4 microA. Sheath gas pressure (high purity liquid nitrogen) was 35 kPa. Mass spectra were obtained over the m/e range of 300 to 900 amu, scan duration of 1s and Q1 peak width at 0.7. The stationary phase was Zorbax Extend C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase: dichloromethane-acetonitrile (35:65), flow rate: 1 mL x min(-1); column temperature: 25 degrees C.


RESULTS
12 triacylglycerols were identified by HPLC-MS method.


CONCLUSIONS
The result can be used to identify the components in a fingerprint chromatogram of coix oil and its related injection product.
Xiang
Zhi-Min
ZM
Zhejiang Provincial Institute for Drug Control, Hangzhou 310004, China. xiangzm@sina.com
Zhu
Ming
M
Chen
Bi-Lian
BL
Chen
Yong
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031742:Coix / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D010938:Plant Oils / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012639:Seeds / Q000737:chemistry; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D010938:Plant Oils; D014280:Triglycerides