Differentiation of monoiodothyronines using electrospray ionization tandem mass spectrometry.
In this work two monoiodothyronines, 3-T1 and 3'-T1, have been analyzed using electrospray ionization tandem mass spectrometry (ESI-MS/MS). Fragmentation patterns were proposed based on our data obtained by ESI-MS/MS. MS2 spectra in either negative or positive ion mode can be used to differentiate 3-T1 and 3'-T1. Based on the relative abundance of fragment ions in MS2 spectra in the negative ion mode, quantification of the 3-T1 and 3'-T1 isomers in mixtures is achieved without prior separation. Solid-phase extraction in combination with ESI-MS/MS provides a practicable procedure that can be used to determine the molar ratio of 3-T1 and 3'-T1 in human serum with an error less than 3%. The detection limits for 3-T1 and 3'-T1 were 0.5 and 0.7 pg/microL, respectively.
Zhang
Yuntao
Y
Division of Biology, Kansas State University, Manhattan, KS 66506-4901, USA. ytz@ksu.edu
Conrad
Abigail H
AH
Thoma
Rudy
R
Conrad
Gary W
GW
D006801:Humans; D007536:Isomerism; D015394:Molecular Structure; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013970:Thyronines / Q000032:analysis* / Q000097:blood / Q000737:chemistry*
D013970:Thyronines; C013068:3-monoiodothyronine