[Regeneration of cybrid Lycopersicon peruvianum x (Solanum rickii) plants with genetically transformed chloroplasts].
The hybrid plants with transformed plastids were regenerated after PEG fusion of chlorophyll-deficient Lycopersicon peruvianum leaf mesophyll protoplasts and leaf mesophyll protoplasts of Solanum rickii, which were previously genetically transformed and as the result were resistant to streptomycine and spectinomycine. The hybrid callus selection was based on the inability of the Lycopersicon peruvianum minicalli to have the green coloration and on the inability of gamma-preirradiated Solanum rickii protoplasts to divide. The hybrids were identified on the base of PCR analyses of nuclear and plastid DNA.
Matveeva
N A
NA
Momot
V P
VP
Shakhovskiĭ
A M
AM
Kuchuk
N V
NV
D002459:Cell Fusion / Q000379:methods; D002467:Cell Nucleus / Q000737:chemistry; D002736:Chloroplasts / Q000737:chemistry / Q000235:genetics* / Q000502:physiology* / Q000528:radiation effects; D004247:DNA / Q000378:metabolism; D005720:Gamma Rays; D006822:Hybrid Cells; D018551:Solanum lycopersicum / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction; D012038:Regeneration / Q000502:physiology*; D012641:Selection, Genetic; D032322:Solanum / Q000235:genetics*; D014170:Transformation, Genetic
D004247:DNA