Fluoro Jade-B detection of dying cells in the SVZ and RMS of adult rats after bilateral olfactory bulbectomy.
A novel fluorochrome, Fluoro-Jade B, was used to detect dying precursor cells in the subventricular zone (SVZ) and rostral migratory stream (RMS) of adult rats after bilateral olfactory bulbectomy and in control intact rats. The animals in experimental group were left to survive 3 days and from 3 till 16 months after surgical procedure. 1. In the control animals, Fluoro-Jade B positive cells were visible in the SVZ and within the whole extent of the RMS. The number of Fluoro-Jade B positive cells increased in the elbow in comparison to the rest parts of the RMS. 2. In the experimental animals surviving either 3 days or from 3 till 16 months after bilateral olfactory bulbectomy, Fluoro-Jade B positive cells displayed the similar pattern of distribution as in the control animals. However, some quantitative differences in the labeled cells number along the rostral migratory pathway appeared. 3. The average number of degenerating cells within the control SVZ and RMS was 26.24+/- 0.686. In bulbectomized animals, regardless of survival time, an insignificant increase of Fluoro-Jade B positive cells number occurred. We can conclude that dying of precursor cells is a physiological process running within the SVZ/RMS in both control and experimental animals. Moreover, this physiological process is not influenced by survival period after bilateral olfactory bulbectomy. Our results demonstrate Fluoro-Jade B as a useful marker of dying cells.
Mitrusková
Barbora
B
Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovak Republic. pousova@saske.sk
Orendácová
Judita
J
Raceková
Enikö
E
D000818:Animals; D017209:Apoptosis; D002452:Cell Count; D002465:Cell Movement; D003714:Denervation; D005452:Fluoresceins; D020547:Lateral Ventricles / Q000473:pathology; D008297:Male; D009410:Nerve Degeneration / Q000473:pathology*; D009474:Neurons / Q000473:pathology*; D009830:Olfactory Bulb / Q000293:injuries*; D009930:Organic Chemicals; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling / Q000379:methods*; D013234:Stem Cells / Q000473:pathology; D013687:Telencephalon / Q000473:pathology; D013997:Time Factors
D005452:Fluoresceins; D009930:Organic Chemicals; C435731:fluoro jade