title: The effect of a mutagen (N-methyl-N-nitro-N-nitrosoguanidine) on cultured cells from adult Schistosoma japonicum.
abstract: The transforming effect of N-methyl-N-nitro-N-nitrosoguanidine (MNNG) on cultured cells from Schistosoma japonicum (S. japonicum) was studied using mono-factor and orthogonal tests. Under the influence of MNNG, cultured cells grew well, and cell survival time was more than 246 days in low-serum medium. When treated with 3 mug/ml MNNG for 48 h, the number of dividing cells increased significantly as determined by bright-field and scanning electron microscopy (SEM). Under these conditions, abundant microvilli, ruffles, microridges, papillae and blebs were observed on the surface of the induced cells. Treatment with MNNG may overcome existing limitations to get continually proliferating schistosome cells and open the possibility to immortalize isolated cells.
authors.affiliation: Department of Medical Parasitology and Research Laboratory of Schistosomiasis, Wuhan University School of Medicine, Wuhan, Hubei Province, 430071, People's Republic of China.
authors.forename: Zhenping
authors.initials: Z
authors.lastname: Ming
authors.forename: Huifen
authors.initials: H
authors.lastname: Dong
authors.forename: Qinping
authors.initials: Q
authors.lastname: Zhong
authors.forename: Christoph G
authors.initials: CG
authors.lastname: Grevelding
authors.forename: Mingsen
authors.initials: M
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000648:ultrastructure; D009153:Mutagens / Q000494:pharmacology*; D012549:Schistosoma japonicum / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D009153:Mutagens; D008769:Methylnitronitrosoguanidine