title: Proteomic analysis of the cerebrospinal fluid of patients with lumbar disk herniation.
abstract: To better understand the pathophysiologic mechanisms underlying spinal nerve root injury induced by lumbar disk herniation (LDH), comparative proteomic analysis of cerebrospinal fluid (CSF) between patients with LDH (the experiment group) and the otherwise healthy patients who had had implants removed from healed fractures in the lower limbs (the control group) was carried out using 2-DE followed by LC-IT-MS and database searching. Image analysis of silver-stained 2-DE gels revealed that 15 protein spots showed significant differential expression between the two groups of CSF samples (p < 0.05). After searching the database we found that in CSF of LDH patients, the expression of cystatin C, apolipoprotein A-IV, vitamin D-binding protein, neurofilament triplet L protein, IgG, tetranectin, and hemoglobin were elevated. However, ProSAAS, prostagladin D2 synthase, creatine kinase B, superoxide dismutase 1 and peroxiredoxin 2 were decreased. The subsequent ELISA measured the concentration of tetranectin, vitamin D-binding protein and cystatin C and confirmed the results of proteomic analysis. These identified proteins are involved in the pathophysiological process of spinal nerve root injury caused by herniated lumbar disk. The functional implications of the alterations in the levels of these proteins are discussed in this paper.
authors.affiliation: Department of Orthopaedic Surgery, Shanghai No. 6 People's Hospital, Shanghai JiaoTong University, Shanghai, P. R. China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Liu
authors.forename: Bing-Fang
authors.initials: BF
authors.lastname: Zeng
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Xu
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Zhu
authors.forename: Qi-Chang
authors.initials: QC
authors.lastname: Xia
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000134:cerebrospinal fluid; D002556:Cerebrospinal Fluid Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007405:Intervertebral Disc Displacement / Q000134:cerebrospinal fluid*; D008159:Lumbar Vertebrae / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins