Reactive oxygen species, cell growth, and taxol production of Taxus cuspidata cells immobilized on polyurethane foam.
Dynamic changes in reactive oxygen species (ROS) of Taxus cuspidata cells immobilized on polyurethane foam were investigated and the relation between ROS content and taxol production was discussed. Immobilization shortened the lag period of cell growth and moderately increased H2O2 and O2-* contents inside the microenvironment within the first 15 d. After 20 d, excessive production of H2O2 and O2-* was observed accompanied by marked increases in membrane lipid peroxidation and cell membrane permeability. The taxol content of immobilized cells was fourfold that of suspended cells at d 35. The addition of exogenous H2O2 barely affected malondialdehyde content and cell membrane permeability but led to an obvious accumulation of taxol. It is inferred that the intracellular and extracellular H2O2 inside the microenvironment might be one factor promoting taxol biosynthesis under the immobilization stress.
Yin
De-Ming
DM
Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin 300072, PR China.
Wu
Jin-Chuan
JC
Yuan
Ying-Jin
YJ
D018929:Cell Culture Techniques; D018914:Cells, Immobilized / Q000378:metabolism; D017239:Paclitaxel / Q000096:biosynthesis*; D011140:Polyurethanes; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020946:Taxus / Q000166:cytology / Q000378:metabolism*
D011140:Polyurethanes; D017382:Reactive Oxygen Species; D017239:Paclitaxel