Effects of antiflammins on transglutaminase and phospholipase A2 activation by transglutaminase.
Two anti-inflammatory peptides, named antiflammins (AFs), corresponding to a region with high amino acid similarity between lipocortin-1 and uteroglobin were tested for their ability to inhibit transglutaminase (TG) and low-molecular-mass phospholipase A2 (PLA2). Porcine pancreatic PLA2 activity and guinea pig hepatic TG activity were determined by arachidonyl release from arachidonyl-phosphatidylcholine and by the incorporation of putrescine into succinylated casein, respectively. AFs inhibited TG activity but did not affect PLA2 activity. Moreover, porcine pancreatic PLA2 was activated by TG and AFs decreased porcine pancreatic PLA2 activation induced by TG. Taken together, our results support the hypothesis that the anti-inflammatory effects of AFs are, at least in part, due to the action of AFs on TG activity.
Moreno
Juan José
JJ
Department of Physiology, Faculty of Pharmacy, Barcelona University, Barcelona, Spain. jjmoreno@ub.edu
D000818:Animals; D017305:Annexin A1 / Q000737:chemistry / Q000494:pharmacology; D016718:Arachidonic Acid / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006168:Guinea Pigs; D008099:Liver / Q000201:enzymology; D009842:Oligopeptides / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D013552:Swine; D011503:Transglutaminases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D014598:Uteroglobin / Q000737:chemistry / Q000494:pharmacology*
D017305:Annexin A1; D004791:Enzyme Inhibitors; D009842:Oligopeptides; D010446:Peptide Fragments; C064645:antiflammin P1; C064647:antiflammin P2; D016718:Arachidonic Acid; D014598:Uteroglobin; D011503:Transglutaminases; D010741:Phospholipases A; D054467:Phospholipases A2