Cloning, characterization and expression of a Lateral suppressor-like gene from chrysanthemum (Dendranthema grandiflorum Kitamura).
Conventionally, the lateral shoots of chrysanthemum are removed manually, which is time consuming and uneconomical. The development of branchless chrysanthemum will economize its commercial cultivation. To investigate the regulatory mechanism of branchlessness, we undertook cloning of Dendranthema grandiflorum Kitamura Lateral suppressor-like (DgLsL) gene for development of lateral shoot in chrysanthemum. A full-length cDNA of DgLsL gene was isolated by screening cDNA library and performing Rapid Amplification of cDNA Ends (RACE) PCR. Phylogenetic analysis showed that the DgLsL gene is closely related to Lateral suppressor that encodes transcriptional regulator proteins belonging to GRAS family of known transcription factor. Southern blot analysis revealed that DgLsL gene in chrysanthemum genome has one copy. DgLsL expression was apparently up-regulated by ethephon treatment. The expression patterns revealed that DgLsL transcripts were detected in all organs, but showed their highest level in stems.
Yang
Dae Hwa
DH
Department of Horticulture, College of Agriculture and Life Science, Chungnam National University, 220 Kung-Dong, Yusong-Gu, Daejeon 305-764, Korea.
Yun
Pil-Yong
PY
Park
So Young
SY
Plaha
Prikshit
P
Lee
Dong Sook
DS
Lee
In Sok
IS
Hwang
Yong Su
YS
Kim
Young A
YA
Lee
Jong Suk
JS
Han
Bong Hee
BH
Lee
Soo Young
SY
Suh
Eun Jung
EJ
Lim
Yong Pyo
YP
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D031202:Chrysanthemum / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D015723:Gene Library; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
D009943:Organophosphorus Compounds; D010937:Plant Growth Regulators; C005073:ethephon