Evolution of EV71 genogroup in Taiwan from 1998 to 2005: an emerging of subgenogroup C4 of EV71.
In Taiwan, enterovirus 71 (EV71) has played an important role in severe enterovirus-related cases every year since the devastating outbreak in 1998. Three genogroups A, B, C occur worldwide; with the B and C genogroups being subdivided into B1-B4 and C1-C4 subgenogroups respectively. To understand the mutation of the EV71 genogroup in Taiwan before and after 1998, a total of 54 worldwide strains were studied including 41 Taiwanese strains obtained in 1986 and 1998-2004. A fragment of 207 bp of the VP4 region was amplified and sequenced. Genetic analysis was performed using MEGA software (version 3.0) for the nucleotide sequence alignment and phylogenetic analysis. In Taiwan, the subgenogroup B1 was predominant before 1998 while subgenogroup C2 was the major etiologic group in 1998 outbreak. A minor etiologic group outbreak in 1998, subgenogroup B4, became predominant during the period from 1999 to 2003. In this study, subgenogroup C4 emerged and became predominant in 2004 in Taiwan. The nucleotide differences between B1 and C2, C2 and B4, B4 and C4 were 20%-26%, 19%-27%, 18%-22%, respectively. Nucleotide sequence alignment revealed 67 substitutions. Most of the substitutions (62/67) were silent mutations. This is the first report about the emergence of EV71 subgenogroup C4 in Taiwan.
Lin
Kuei-Hsiang
KH
Department of Clinical Laboratory, Kaohsiung Medical University, Kaohsiung City, Taiwan. kuhsli@ms3.hinet.net
Hwang
Kao-Pin
KP
Ke
Guan-Ming
GM
Wang
Chu-Feng
CF
Ke
Liang-Yin
LY
Hsu
Yu-Ting
YT
Tung
Yi-Chin
YC
Chu
Pei-Yu
PY
Chen
Bai-Hsiun
BH
Chen
Hsiu-Lin
HL
Kao
Chuan-Liang
CL
Wang
Jen-Ren
JR
Eng
Hock-Liew
HL
Wang
Sheng-Yu
SY
Hsu
Li-Ching
LC
Chen
Hour-Young
HY
D001483:Base Sequence; D004196:Disease Outbreaks; D004770:Enterovirus / Q000235:genetics*; D004769:Enterovirus Infections / Q000453:epidemiology*; D019143:Evolution, Molecular; D005814:Genes, Viral; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013045:Species Specificity; D013624:Taiwan / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins