title: Quantitative analysis of trimethylsilyl derivative of hydroxyurea in plasma by gas chromatography-mass spectrometry.
abstract: Hydroxyurea is an antitumor drug widely used in the treatment of sickle cell disease. The drug has been analyzed in biological fluids by a number of high-performance liquid chromatography (HPLC) methods. This paper describes a fast and highly reliable capillary gas chromatography-mass spectrometry (GC-MS) procedure that was developed for the detection and quantitation of hydroxyurea in plasma. The compound and its labeled internal standard were liquid extracted from plasma and derivatized with BSTFA before analysis. The detection limit of the assay was 0.078 microg/ml and the limit of quantitation was 0.313 microg/ml with linearity up to 500 microg/ml. Intra-day variation, as coefficient of variation (C.V., %) over the selected concentration range, was 0.3-8.7% and inter-day variation was 0.4-9.6%.
authors.affiliation: Department of Pharmacology, College of Medicine, Howard University, 520 W Street N.W., Washington, DC 20059, USA. HUJamesell@aol.com
authors.forename: Hutchinson
authors.initials: H
authors.lastname: James
authors.forename: Masoud
authors.initials: M
authors.lastname: Nahavandi
authors.forename: Melville Q
authors.initials: MQ
authors.lastname: Wyche
authors.forename: Robert E
authors.initials: RE
authors.lastname: Taylor
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006918:Hydroxyurea / Q000031:analogs & derivatives* / Q000097:blood*; D012680:Sensitivity and Specificity; D014297:Trimethylsilyl Compounds / Q000097:blood* / Q000737:chemistry
chemical_list: D014297:Trimethylsilyl Compounds; C047270:N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide; D006918:Hydroxyurea