title: G-CSF enhances the adhesion of encephalitogenic T cells to extracellular matrix components: a possible mechanism for exacerbation of multiple sclerosis.
abstract: Autologous stem cell transplantation is being considered as treatment of severe refractory autoimmune disorders, including MS. Stem cell mobilization is achieved with granulocyte-colony stimulating factor (G-CSF), however, G-CSF administration resulted in cases of worsened clinical MS status. We studied autoreactive T-cell properties, which can promote CNS inflammation in MS. We show that G-CSF enhances MS autoreactive T cell line adhesion to the ECM proteins collagen IV and fibronectin as effectively as the proinflammatory IFNgamma and TNFalpha, known to exacerbate MS symptoms. We propose a link between clinical worsening of MS symptoms induced by G-CSF and the hyperstimulation of T cell adhesion to ECM elicited by G-CSF.
authors.affiliation: Faculty of Life Science, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Omri
authors.initials: O
authors.lastname: Snir
authors.forename: Gad
authors.initials: G
authors.lastname: Lavie
authors.forename: Anat
authors.initials: A
authors.lastname: Achiron
authors.forename: Ilan
authors.initials: I
authors.lastname: Bank
authors.forename: Taya
authors.initials: T
authors.lastname: Ben-Aharon
authors.forename: Benjamin
authors.initials: B
authors.lastname: Sredni
authors.forename: Irun R
authors.initials: IR
authors.lastname: Cohen
authors.forename: Mathilda
authors.initials: M
authors.lastname: Mandel
mesh_terms: D015153:Blotting, Western / Q000379:methods; D002448:Cell Adhesion / Q000187:drug effects*; D002452:Cell Count / Q000379:methods; D016207:Cytokines / Q000378:metabolism / Q000494:pharmacology; D004843:Epistasis, Genetic; D005109:Extracellular Matrix / Q000378:metabolism*; D051418:Focal Adhesion Kinase 2 / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D006801:Humans; D039222:Integrin alpha1beta1 / Q000378:metabolism; D009103:Multiple Sclerosis / Q000473:pathology*; D004676:Myelin Basic Protein / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects*; D013997:Time Factors
chemical_list: D016207:Cytokines; D039222:Integrin alpha1beta1; D004676:Myelin Basic Protein; D016179:Granulocyte Colony-Stimulating Factor; D051418:Focal Adhesion Kinase 2