The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus.
To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuB(KU80)). Homologous integration reached about 80% for both calcineurin A (calA) and polyketide synthase pksP (alb1) genes in the akuB(KU80) mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuB(KU80) had no influence on pathogenicity in a low-dose murine infection model.
da Silva Ferreira
Márcia Eliana
ME
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Ciências Farmacêuticas, Universidade de São Paulo, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil.
Kress
Marcia R V Z
MR
Savoldi
Marcela
M
Goldman
Maria Helena S
MH
Härtl
Albert
A
Heinekamp
Thorsten
T
Brakhage
Axel A
AA
Goldman
Gustavo H
GH
D000818:Animals; D034961:Antigens, Nuclear / Q000235:genetics*; D001232:Aspergillus fumigatus / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D019703:Calcineurin / Q000172:deficiency / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005821:Genetic Techniques; D016681:Genome, Fungal; D000072200:Ku Autoantigen; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D011995:Recombination, Genetic / Q000235:genetics*; D014774:Virulence
D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D008741:Methyl Methanesulfonate; D019703:Calcineurin; C000606510:Xrcc6 protein, mouse; D000072200:Ku Autoantigen