title: Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods.
abstract: Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates from a woman with unknown HIV status, had been identified as belonging to the Mycobacterium avium complex by conventional procedures. In both patients, using genetic analysis procedures such as PCR-restriction enzyme analysis (PRA) of the hsp65 gene, a commercially available reverse hybridization-based assay (INNO-LiPA mycobacteria) and/or sequencing analysis of the 16S-23S internal transcribed spacer (ITS), the presence of Mycobacterium lentiflavum was also demonstrated. At the time of detection, both cases were also infected with M. avium, suggesting an underestimation of infection with M. lentiflavum and co-infection with different Mycobacterium species.
authors.forename: Philip
authors.initials: P
authors.lastname: Suffys
authors.forename: Adalgiza
authors.initials: A
authors.lastname: da Silva Rocha
authors.forename: Adeilton
authors.initials: A
authors.lastname: Brandão
authors.forename: Bart
authors.initials: B
authors.lastname: Vanderborght
authors.affiliation: Innogenetics N. V., Technologiepark 6, B-9052 Ghent, Belgium.
authors.forename: Wouter
authors.initials: W
authors.lastname: Mijs
authors.affiliation: Innogenetics N. V., Technologiepark 6, B-9052 Ghent, Belgium.
authors.forename: Geert
authors.initials: G
authors.lastname: Jannes
authors.forename: Fernanda C Q
authors.initials: FCQ
authors.lastname: Mello
authors.affiliation: Adolfo Lutz Institute, São Paulo, Brazil.
authors.forename: Heloisa da Silveira Paro
authors.initials: HDSP
authors.lastname: Pedro
authors.forename: Leila
authors.initials: L
authors.lastname: de Souza Fonseca
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Paulista School of Medicine, São Paulo, Brazil.
authors.forename: Rosângela Siqueira
authors.initials: RS
authors.lastname: de Oliveira
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Paulista School of Medicine, São Paulo, Brazil.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Cardoso Leão
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Féres Saad
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics; D001769:Blood / Q000382:microbiology; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005821:Genetic Techniques; D005838:Genotype; D015658:HIV Infections / Q000150:complications; D006801:Humans; D008168:Lung / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000150:complications* / Q000382:microbiology; D015269:Mycobacterium avium Complex / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015270:Mycobacterium avium-intracellulare Infection / Q000150:complications* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins