[Study on the effect and its mechanisms of 5F from Pteri semipinnata L. on the cell cycle of highly metastatic ovarian carcinoma HO-8910PM cells].
OBJECTIVE
To study the effect and its possible mechanism of 5F from Pteri semipinnata L on the cell cycle of human highly metastatic ovarian carcinoma HO-8910PM cells.


METHODS
MTT assay was used to examine the effect of 5F on proliferation of HO-8910PM cells after 24 hours treatment; The cell cycle was assessed by flow cytometry (FCM); The expression level of NF-kappaB (p65) FAK and the level of phosphorylated FAK were assessed by Western blot analysis.


RESULTS
5F from Pteris semipinnata L. could inhibit the proliferation of HO-8910PM cell and block the cell cycle at G2/M phase. The expression level of NF-kappaB (P65) protein decreased obviously in HO-8910PM cells treated with 25 - 100 micromol/L 5F for 24 hours, and the effect appeared in a dose-dependent manner. 5F up-regulated significantly the expression of FAK, down-regulated the level of phosphorylated FAK.


CONCLUSIONS
The effect on cell cycle of 5F from Pteri semipinnata L is involved in the expression of NF-kappaF(p65), FAK and the level of phosphorylated FAK.
He
Taipingi
T
Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang, Guangdong.
He
Zhenhui
Z
Mo
Lier
L
Liang
Nianci
N
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004224:Diterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D006801:Humans; D016328:NF-kappa B / Q000096:biosynthesis; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D010946:Plants, Medicinal / Q000737:chemistry; D032495:Pteris / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D016328:NF-kappa B; D051417:Focal Adhesion Kinase 1