Asr1, an alcohol-responsive factor of Saccharomyces cerevisiae, is dispensable for alcoholic fermentation.
Yeast Asr1 is the first reported protein whose intracellular distribution changes specifically in response to alcohol (Betz et al. (2004) J Biol Chem 279:28174-28181). It was reported that Asr1 is required for tolerance to alcohol and plays an important role in the alcohol stress response. Therefore, Asr1 is of interest to brewers and winegrowers attempting to improve the techniques of alcoholic fermentation. We verified the importance of Asr1 in the alcohol stress response during alcoholic fermentation. Although we reconfirmed the alcohol-responsive changes in the intracellular localization of Asr1, we could not detect the effects of Asr1-deficiency on Japanese sake brewing or winemaking. In addition, we could not reconfirm the hypersensitivity of Asr1-deficient mutants to alcohol and sodium dodecyl sulfate. Instead, we conclude that Asr1 is not required and nor important for tolerance to alcohol stress.
Izawa
Shingo
S
Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan. izawa@kais.kyoto-u.ac.jp
Ikeda
Kayo
K
Kita
Takeomi
T
Inoue
Yoshiharu
Y
D021581:Active Transport, Cell Nucleus; D048868:Adaptor Proteins, Signal Transducing; D000434:Alcoholic Beverages / Q000382:microbiology; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D005285:Fermentation / Q000502:physiology; D017353:Gene Deletion; D049452:Green Fluorescent Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*
D048868:Adaptor Proteins, Signal Transducing; C488254:Asr1 protein, S cerevisiae; D002352:Carrier Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins