title: Molecular detection of circulating cancer cells in the peripheral blood of patients with colorectal cancer by using membrane array with a multiple mRNA marker panel.
abstract: The objective of this study was mainly to evaluate the simultaneous detection of expression levels of a multiple mRNA marker panel in the peripheral blood of colorectal cancer (CRC) patients for use in complementary CRC diagnosis. Twenty-seven tumor tissue specimens and 80 peripheral blood specimens were collected from CRC patients. Firstly, the levels of multiple molecular markers in the tumor tissue and blood specimens were evaluated by using real-time quantitative PCR (RT-QPCR) and membrane array. The result of linear regression showed a high degree of correlation (r=0.954, P<0.0001) between the data of these two methods. CK-19 was the marker with the highest detection rate (87.5%) in the peripheral blood, followed by CEA (82.6%), REG4 (80.8%), and then uPA (80.0%) and TLAM1 (80.0%). The levels of the six markers in the peripheral blood were extensively explored. In the 80 patients, the frequency of CK-19, CK-20, CEA, REG4, uPA, and TIAM1 mRNA overexpression was 82.5% (66/80), 78.8% (63/80), 82.5% (66/80), 80.0% (64/80), 78.8% (63/80), and 80.0% (64/80), respectively. Then, a panel combining these 6 mRNA markers was evaluated for its utility in the clinical diagnosis of CRC. The sensitivity, specificity, and accuracy of membrane array-based diagnostic method were 88.8%, 87.8%, and 88.2%, respectively; much higher than those of examinations with single markers. Finally, lymph node metastasis (P=0.024) and TNM stage (P=0.009) were found to be significantly correlated with overexpression of the multiple mRNA marker panel. The detection rates of stage-I and -II CRC by using the multi-marker membrane array were 54.5% (6/11) and 92.0% (23/25), respectively. In conclusion, the results of the present study have shown that this innovative membrane array technique with a multiple mRNA marker panel can significantly improve the diagnosis rate of early colorectal cancer.
authors.affiliation: MedicoGenomic Research Center, Kaohsiung Medical University, Kaohsiung 80317, Taiwan, ROC.
authors.forename: Ching-Sheng
authors.initials: CS
authors.lastname: Yeh
authors.forename: Jaw-Yuan
authors.initials: JY
authors.lastname: Wang
authors.forename: Chan-Han
authors.initials: CH
authors.lastname: Wu
authors.forename: Inn-Wen
authors.initials: IW
authors.lastname: Chong
authors.forename: Fu-Yen
authors.initials: FY
authors.lastname: Chung
authors.forename: Yung-Hsin
authors.initials: YH
authors.lastname: Wang
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Yu
authors.forename: Shiu-Ru
authors.initials: SR
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D002272:Carcinoembryonic Antigen / Q000097:blood / Q000235:genetics; D002277:Carcinoma / Q000097:blood* / Q000235:genetics / Q000473:pathology; D015179:Colorectal Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology; D042241:Early Diagnosis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053539:Keratin-19 / Q000097:blood / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating / Q000737:chemistry / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000097:blood; D012372:ROC Curve; D012680:Sensitivity and Specificity; D014568:Urokinase-Type Plasminogen Activator / Q000097:blood / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D053539:Keratin-19; D012333:RNA, Messenger; D014568:Urokinase-Type Plasminogen Activator