title: Transgenic tobacco (Nicotiana tabacum L. cv. Samsun-NN) plants over-expressing a synthetic HRP-C gene are altered in growth, development and susceptibility to abiotic stress.
abstract: The physiological role of class III peroxidases (EC 1.11.1.7) in controlling plant growth and development has been investigated by over-expression of both native and heterologous peroxidases. However, it has remained an enigma as to why the phenotypes of different peroxidase over-expressing transgenics vary. In order to resolve the conflicting information about the consequences of peroxidase over-expression, we have explored the role of the subcellular targeting of HRP-C in controlling stem growth, root development, axillary branching and abiotic stress tolerance in tobacco (Nicotiana tabacum L.). Altering the sub-cellular targeting of vacuolar HRP-C, such that over-expressed peroxidase accumulates in the cytoplasm and cell wall, induced phenotypic changes that are typically associated with altered auxin homeostasis, and over-expression of cell wall located peroxidases. We conclude that sub-cellular targeting is a determinant of the phenotype of peroxidase over-expressing plants.
authors.affiliation: Plant Cell Culture Unit, National University of Ireland, Maynooth, Co. Kildare, Ireland.
authors.forename: Laura
authors.initials: L
authors.lastname: Heggie
authors.forename: Marcel A K
authors.initials: MA
authors.lastname: Jansen
authors.forename: Emma M
authors.initials: EM
authors.lastname: Burbridge
authors.forename: Tony A
authors.initials: TA
authors.lastname: Kavanagh
authors.forename: Roger N F
authors.initials: RN
authors.lastname: Thorneley
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Dix
mesh_terms: D002473:Cell Wall / Q000502:physiology; D003593:Cytoplasm / Q000502:physiology; D017343:Genes, Plant; D005813:Genes, Synthetic; D006735:Horseradish Peroxidase / Q000096:biosynthesis* / Q000235:genetics; D007210:Indoleacetic Acids / Q000378:metabolism; D008031:Lignin / Q000096:biosynthesis; D045332:Photosystem II Protein Complex / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014466:Ultraviolet Rays
chemical_list: D007210:Indoleacetic Acids; D045332:Photosystem II Protein Complex; D008031:Lignin; D006735:Horseradish Peroxidase