[Alteration of hydrogen sulfide/cystathionine-beta-synthase system in rats with recurrent febrile seizures].
OBJECTIVE
To study the alteration of hydrogen sulfide (H(2)S)/ cystathionine-beta-synthase (CBS) system during recurrent febrile seizures (FS) in the hippocampus of developing rats.


METHODS
The rats were randomly divided into control group (n=8) and hyperthermia-treated group (n=22). Which was subdivided into FS group (n=8) and H group(no seizure occurred, n=9) according to whether seizures occurred. The plasma level of H(2)S was detected by the spectrophotometer. The expression levels of CBS gene and protein were examined by in situ hybridization and immunohistochemistry respectively.


RESULTS
The plasma levels of H(2)S were increased significantly in FS group compared with those of control group or H group. The expression levels of CBS gene and protein were enhanced in FS group compared with those of control group or H group.


CONCLUSIONS
The expression levels of H(2)S/ CBS system were up-regulated during recurrent FS.
Han
Ying
Y
Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.
Qin
Jiong
J
Chang
Xing-zhi
XZ
Yang
Zhi-xian
ZX
Du
Jun-bao
JB
D000818:Animals; D003541:Cystathionine beta-Synthase / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D006862:Hydrogen Sulfide / Q000097:blood*; D006979:Hyperthermia, Induced; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012008:Recurrence; D003294:Seizures, Febrile / Q000097:blood / Q000235:genetics / Q000503:physiopathology*; D013053:Spectrophotometry; D015854:Up-Regulation
D003541:Cystathionine beta-Synthase; D006862:Hydrogen Sulfide