Quantum dots with a paramagnetic coating as a bimodal molecular imaging probe.
MRI detectable and targeted quantum dots were developed. To that aim, quantum dots were coated with paramagnetic and pegylated lipids, which resulted in a relaxivity, r(1), of nearly 2000 mM(-1)s(-1) per quantum dot. The quantum dots were functionalized by covalently linking alphavbeta3-specific RGD peptides, and the specificity was assessed and confirmed on cultured endothelial cells. The bimodal character, the high relaxivity, and the specificity of this nanoparticulate probe make it an excellent contrast agent for molecular imaging purposes.
Mulder
Willem J M
WJ
Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands. w.j.m.mulder@tue.nl
Koole
Rolf
R
Brandwijk
Ricardo J
RJ
Storm
Gert
G
Chin
Patrick T K
PT
Strijkers
Gustav J
GJ
de Mello Donegá
Celso
C
Nicolay
Klaas
K
Griffioen
Arjan W
AW
D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism; D019786:Gadolinium DTPA; D006801:Humans; D007089:Image Enhancement; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D008081:Liposomes; D008279:Magnetic Resonance Imaging; D008856:Microscopy, Fluorescence; D015335:Molecular Probes; D009842:Oligopeptides / Q000378:metabolism*; D010714:Phosphatidylethanolamines; D011092:Polyethylene Glycols; D045663:Quantum Dots; D014471:Umbilical Veins / Q000166:cytology
D039302:Integrin alphaVbeta3; D008081:Liposomes; D015335:Molecular Probes; D009842:Oligopeptides; D010714:Phosphatidylethanolamines; C077628:gadolinium stearylamide DTPA; C107570:polyethylene glycol-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D019786:Gadolinium DTPA