[Study on the burden of abnormal hematopoietic clone of the patients with myelodysplastic syndromes and its implications].
OBJECTIVE
To investigate the abnormal hematopoietic clone burden of the patients with myelodysplastic syndromes (MDS) and its clinical implication.


METHODS
The ratio of the metaphase with abnormal karyotypes to the total was regarded as the index of MDS clonal burden. Thirteen parameters were assayed and the correlations between these parameters and MDS clone burden were analysed.


RESULTS
The clonal burden of MDS patients was (67.4 +/- 36.2)%. It correlated positively with bone marrow blasts (r = 0.483, P < 0.05), negatively with hemoglobin level (r = -0.445, P < 0.05). The number of blasts, hemoglobin and erythrocytes in high clonal burden (>50%) and low clonal burden (< or = 50%) groups were significantly different (P < 0.05). CD4+ T lymphocytes of MDS patients and normal controls were (274.18 +/-71.85) x 10(6)/L and (454.82 +/- 205.88) x 10(6)/L (P < 0.05) respectively. CD8+ T lymphocytes between MDS patients and normal controls had no difference. The serum level of IL-2 of MDS patients and normal control groups were (6.29 +/- 3.58) g/L and (3.11 +/- 1.40) microg/L (P < 0.05) respectively; but no difference in the serum level of TNF between MDS and control groups. The ratio of CD4+ to CD8+ in high clonal burden patients was 1.90 + 0.52, and in low clonal burden patients was 0.97 +/- 0.44 (P < 0.05).


CONCLUSIONS
The clonal burden and deficient T cell immunity are the indicators for predicting MDS patients clinical progression.
Wang
Hua-Quan
HQ
Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin, China.
Shao
Zong-Hong
ZH
Shi
Jun
J
Cao
Yan-Ran
YR
Liu
Hong
H
Bai
Jie
J
Tu
Mei-Feng
MF
Xing
Li-Min
LM
Cui
Zhen-Zhu
ZZ
Liu
Shi-He
SH
Sun
Juan
J
Jia
Hai-Rong
HR
Yang
Tian-Ying
TY
D000293:Adolescent; D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000473:pathology*; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology