The transcription profile of Aleutian mink disease virus in CRFK cells is generated by alternative processing of pre-mRNAs produced from a single promoter.
A reevaluation of the transcription profile of Aleutian mink disease parvovirus (AMDV)-infected CRFK cells at either 32 degrees C or 37 degrees C has determined that strain AMDV-G encodes six species of mRNAs produced by alternative splicing and alternative polyadenylation of a pre-mRNA generated by a single promoter at the left end of the genome. Three different splicing patterns are used, and each type is found polyadenylated at either the 3' end of the genome (the distal site) or at a site in the center of the genome (the proximal site). All spliced species accumulate similarly over the course of infection, with the R2 RNA predominant throughout. The R2 RNA, which contains and can express the NS2 coding region, encodes the viral capsid proteins VP1 and VP2.
Qiu
Jianming
J
Department of Molecular Microbiology and Immunology, University of Missouri-Columbia, School of Medicine, Life Sciences Center, 1201 E. Rollins Rd., Columbia, MO 65211-7310, USA. qiuj@missouri.edu
Cheng
Fang
F
Burger
Lisa R
LR
Pintel
David
D
D000454:Aleutian Mink Disease Virus / Q000235:genetics* / Q000378:metabolism; D017398:Alternative Splicing; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D002460:Cell Line; D016679:Genome, Viral; D026723:Polyadenylation; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D013696:Temperature; D014158:Transcription, Genetic
D036022:Capsid Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; C442555:VP2 protein, Aleutian mink disease virus