title: [Relationship between HLA-DR4/1 subtypes and T cell responses to type II collagen or anti-C II 263-272 antibodies].
abstract: OBJECTIVE
  To investigate the relationship between HLA-DR4/1 subtypes and T cell responses to type II collagen or anti-CII 263-272 antibodies.
  
  
  METHODS
  Peripheral blood mononuclear cells and sera were obtained from 70 rheumatoid arthritis (RA) patients. T cell proliferative responses to CII 263-272 were evaluated by [(3)H] thymidine incorporation assay. Antibodies specific to CII 263-272 were determined by ELISA. HLA-DR4/1 subtypes were analyzed by PCR-SSP.
  
  
  RESULTS
  In HLA-DR4/1 positive group, T cell proliferative responses to CII 263-272 were observed in 55.6% RA patients and anti-CII 263-272 antibodies were present in 66.7% RA patients. In HLA-DR4/1 negative group, T cell proliferative responses to CII 263-272 were observed in 30.3% RA patients and anti-C II 263-272 antibodies were present in 34.8% RA patients. The number of positive antibodies to CII 263-272 or T cell proliferative responses to CII 263-272 in HLA-DR4/1 positive group were higher than in negative group.
  
  
  CONCLUSIONS
  There is a relationship between HLA-DR4/1 subtypes and T cell responses to CII 263-272 or anti-CII 263-272 antibodies.
authors.affiliation: Department of Rheumatology and Immunology, Peking Univesity Peopleos Hospital, Beijing 100044, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Ru
authors.initials: R
authors.lastname: Li
authors.forename: Xin-rong
authors.initials: XR
authors.lastname: Ning
authors.forename: Zhan-guo
authors.initials: ZG
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D024043:Collagen Type II / Q000276:immunology*; D005260:Female; D015804:HLA-DR4 Antigen / Q000097:blood / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D024043:Collagen Type II; D015804:HLA-DR4 Antigen