Isosorbide delays gentamicin-induced vestibular sensory cell death.
The efficacy of isosorbide for protection from vestibular sensory cell damage was investigated. The effects of isosorbide on gentamicin-induced production of nitric oxide (NO) and reactive oxygen species (ROS) were studied by means of the fluorescence indicators 4,5-diaminofluorescein diacetate and dihydrotetramethylrosamine. The effect on gentamicin-induced vestibular sensory cell damage was examined by using an in vitro LIVE/DEAD system. Isosorbide inhibited the production of both NO and ROS. Isosorbide limited the vestibular sensory cell damage caused by gentamicin. It is, therefore, suggested that isosorbide may help to treat inner ear disorders.
Takumida
Masaya
M
Department of Otolaryngology, Hiroshima University Faculty of Medicine, Hiroshima, Japan. masati@hiroshima-u.ac.jp
Anniko
Matti
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D004234:Diuretics, Osmotic / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005839:Gentamicins / Q000633:toxicity*; D006168:Guinea Pigs; D018069:Hair Cells, Vestibular / Q000187:drug effects*; D007547:Isosorbide / Q000494:pharmacology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism
D000900:Anti-Bacterial Agents; D004234:Diuretics, Osmotic; D016166:Free Radical Scavengers; D005839:Gentamicins; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D007547:Isosorbide