title: [Effect of procyanidolic oligomers on corneal collagen fibrillogenesis].
abstract: OBJECTIVE
  We showed in a previous study that procyanidolic oligomers (PCOs) from grape seeds effectively protect corneal stroma against degradation by bacterial collagenase. Here we report the study of the effect of PCOs on protein and collagen composition of cornea and on the biosynthesis of corneal collagens.
  
  
  METHODS
  Bovine corneas were used in explant cultures. We quantitatively determined total proteins and collagen as well as the incorporation of 3H-proline in separated collagen types. Collagens type I, V, and VI were separated and quantitated. In order to understand some of the results obtained, we studied the interaction of PCO with collagen type I separately.
  
  
  RESULTS
  In the absence of PCOs, collagen typing and 3H-proline incorporation yielded the expected results for a normal cornea, with the usual proportion of the three major types of collagens. In presence of PCOs at 1 mg/ml and after 24 h incubation, total proteins and collagens decreased, as did papain-extractable collagens. Proteins in the final residue solubilized in 1 M KOH-80% v/v aqueous ethanol increased. The proportion of the three principal collagens was also modified: type I became preponderant, and the proportions of the two others (type V and VI) decreased. The study of the interaction of collagen type I with PCOs showed that 30% of total PCOs do not interact with collagen, 20% interact reversibly, and 50% of PCOs are strongly and irreversibly fixed. This strongly fixed fraction could not be separated from collagen by either column chromatography or collagenase or KOH in aqueous ethanol.
  
  
  CONCLUSIONS
  Bovine corneas in explant cultures in presence of PCOs undergo a modification of their protein and collagen content, with a concurrent modification of the proportion of collagens types I, V, and VI. Collagen type I predominates, and the two other types decrease. Study of the collagen type I-PCO interaction showed that roughly 50% of PCOs become irreversibly fixed to collagen, resisting collagenase or other methods of separation. This strong PCO-collagen type I interaction explains the decrease in solubility.
authors.affiliation: Laboratoire de Recherche Ophtalmologique, Hôpital Hôtel Dieu, Paris. robertam2@wanadoo.fr
authors.forename: A M
authors.initials: AM
authors.lastname: Robert
authors.forename: L
authors.initials: L
authors.lastname: Robert
authors.forename: G
authors.initials: G
authors.lastname: Renard
mesh_terms: D000818:Animals; D044946:Biflavonoids / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D002417:Cattle; D003315:Cornea / Q000187:drug effects* / Q000378:metabolism*; D024022:Fibrillar Collagens / Q000096:biosynthesis*; D044945:Proanthocyanidins / Q000494:pharmacology*
chemical_list: D044946:Biflavonoids; D024022:Fibrillar Collagens; D044945:Proanthocyanidins; C479585:procyanidol oligomer; D002392:Catechin