title: Genotoxicity of vesicular fluid and saline extract of Taenia solium metacestodes in somatic cells of Drosophila melanogaster.
abstract: Neurocysticercosis, the most common parasitic disease of the central nervous system, is caused by cysticerci of the helminth Taenia solium, which is prevalent in developing countries and is reemerging in affluent societies. This helminth is associated with brain tumors and hematological malignancies in humans. In the present study, we analyzed the genotoxicity of vesicular fluid (VF) and a saline extract (SE) of T. solium metacestodes in the Drosophila melanogaster wing somatic mutation and recombination test (SMART). Third-instar larvae derived from standard and high bioactivation crosses were treated for approximately 48 hr with 12.5, 25.0, and 50.0 microg/ml of VF and SE of T. solium metacestodes. Negative (phosphate buffered saline) and positive (10 mM urethane) controls were also included. The results showed that the two test compounds were genotoxic in both crosses of Drosophila. Nevertheless, further research is needed to determine the genotoxic potential of specific compounds present in VF and SE and their role in the development of cancer.
authors.affiliation: Institute of Biomedical Science, Federal University of Uberlândia, Uberlândia (MG), Brazil.
authors.forename: Luciana Pereira
authors.initials: LP
authors.lastname: Silva
authors.forename: Julia Maria
authors.initials: JM
authors.lastname: Costa-Cruz
authors.forename: Mário Antônio
authors.initials: MA
authors.lastname: Spanó
authors.forename: Ulrich
authors.initials: U
authors.lastname: Graf
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D005260:Female; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D011995:Recombination, Genetic; D012965:Sodium Chloride / Q000737:chemistry; D041201:Taenia solium / Q000737:chemistry*; D014921:Wings, Animal / Q000187:drug effects*
chemical_list: D009153:Mutagens; D012965:Sodium Chloride