[Genetics subtypes and distribution of Seoul virus in Henan].
OBJECTIVE
To investigate the sub-genotypes and distribution ot Seoul virus in Henan.


METHODS
Rodents were collected in the major epidemic areas and rats lungs were studied by indirect immunofluorescence assay. Partial M and S segments were amplified with nested reverse transcription-polymerase chain reaction using Hantavirus genotype-specific primers, sequenced, analyzed and compared with other known sequences.


RESULTS
The Hantavirus carried by Rattus norvegicus, Rattus flavipectus and Mus musculus were all belonged to Seoul virus in the main epidemic areas of Henan. We constructed two phylogenetic tree based on the partial M and S segment sequences while phylogenetic analysis distinguished three genetic subtypes (S1, S2 and S3). S1 and S3 were found main subtypes in Henan.


CONCLUSIONS
The results indicated that the genetic subtypes of Hantavirus were complicated and widely distributed in Henan.
Sun
Li
L
College of Liffe Science, Xinjiang Shihezi University, Shihezi 823003, China.
Zhang
Yong-zhen
YZ
Li
Lin-hong
LH
Zhang
Yan-ping
YP
Zhang
Ai-mei
AM
Hao
Zong-yu
ZY
Sun
Jian-wei
JW
Chen
Hua-xin
HX
D000818:Animals; D002681:China; D010802:Phylogeny; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029263:Seoul virus / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA