Vitellogenin induction in the endangered goodeid fish Girardinichthys viviparus: vitellogenin characterization and estrogenic effects of polychlorinated biphenyls.
Vitellogenin (VTG) is a widely used biomarker in studies of endocrine disruption induced by xenobiotics such as polychlorinated biphenyls (PCBs). This study evaluates the estrogenic effects of these compounds on the black-fin goodeid Girardinichthys viviparus, an endangered fish species in Mexico with a reduced range of distribution due to pollution of its natural environment. Adult fish born in the laboratory were exposed to half the LC(0) of Inerteen commercial PCB mixture. VTG was determined through an inhibition enzyme-linked immunosorbent assay (ELISA) using a homologous-heterologous system. Male and female fish were killed after 1, 2, 4, 8 and 16 days of exposure. The distal third of each specimen was used for analysis. VTG was obtained from cultured hepatocytes and blood serum of males previously exposed to 17beta-estradiol. VTG molecular mass was 348 kDa. PCBs were found to elicit greater estrogenic effects on VTG induction in males than in females (p<0.05) and sex differences were noted. Time-dependent VTG induction kinetics in males and a stationary phase in females were also observed.
Vega-López
Armando
A
Laboratorio de Toxicología Acuática, Escuela Nacional de Ciencias Biológicas, IPN. Prol. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles "Casco de Santo Tomás", D.F. CP 11340, México. Electronic address: avegadv@terra.com.
Martínez-Tabche
Laura
L
Laboratorio de Toxicología Acuática, Escuela Nacional de Ciencias Biológicas, IPN. Prol. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles "Casco de Santo Tomás", D.F. CP 11340, México.
Domínguez-López
Maria Lilia
ML
Laboratorio de Inmunoquímica, Escuela Nacional de Ciencias Biológicas, IPN. Prol. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles "Casco de Santo Tomás", D.F. CP 11340, México.
García-Latorre
Ethel
E
Laboratorio de Inmunoquímica, Escuela Nacional de Ciencias Biológicas, IPN. Prol. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles "Casco de Santo Tomás", D.F. CP 11340, México.
Ramón-Gallegos
Eva
E
Laboratorio de Citopatología Ambiental. Escuela Nacional de Ciencias Biológicas, IPN. Prol. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles "Casco de Santo Tomás", D.F. CP 11340, México.
García-Gasca
Alejandra
A
Laboratorio de Biología Molecular, CIAD Mazatlán. Mazatlán Sinaloa, México.
D000818:Animals; D002478:Cells, Cultured; D003532:Cyprinodontiformes / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000633:toxicity*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D022781:Hepatocytes / Q000378:metabolism; D007106:Immune Sera / Q000096:biosynthesis / Q000276:immunology; D008297:Male; D017686:Oncorhynchus mykiss; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011817:Rabbits; D012737:Sex Factors; D013997:Time Factors; D014819:Vitellogenins / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D052244:Endocrine Disruptors; D007106:Immune Sera; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004958:Estradiol; D011078:Polychlorinated Biphenyls