Detection of foot-and-mouth disease virus infection in vaccinated cattle.
The aim of this study was to evaluate the value of commercially available kits for the detection of foot-and-mouth disease (FMD) virus infection in vaccinated cattle. The cattle were vaccinated with a commercial aqueous FMD vaccine type A24 and subsequently challenged 28 days post vaccination with homologous FMD virus. Seven of eight animals were protected from clinical disease and all became carriers. They were bled sequentially for up to 130 days post infection and samples of sera were tested with three ELISA kits: CHEKIT FMD-3ABC, Ceditest FMDV-NS and SVANOIR FMDV 3ABC-Ab ELISA. The Ceditest kit appears to be relatively higher sensitive than the others. When examined with this ELISA, all cattle developed of FMDV nonstructural proteins (NSPs) antibodies and remained positive throughout the period of the experiment. The response of antibodies against 3ABC antigen delayed in two cattle challenged with FMDV A24 virus. One of the cattle reacted negatively in Svanoir ELISA kit and sera from two animals were found negative in CHEKIT ELISA. It can be concluded that all tested kits can be a promising tool for FMD control and eradication campaigns in situation where emergency vaccination was applied.
Niedbalski
W
W
Department of Foot-and-Mouth Disease of the National Veterinary Research Institute, Zduńska Wola, Poland. wieslaw@piwzp.invar.net.pl
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology / Q000517:prevention & control* / Q000821:virology*; D005536:Foot-and-Mouth Disease / Q000175:diagnosis / Q000276:immunology / Q000517:prevention & control* / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D017361:Viral Nonstructural Proteins / Q000097:blood; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
C109754:3ABC protein, virus; D000914:Antibodies, Viral; D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines