Promotion of cell affinity of porous PLLA scaffolds by immobilization of RGD peptides via plasma treatment.
In the present work, RGDS (Arg-Gly-Asp-Ser) was immobilized on PLLA scaffolds with plasma treatment. The amount of immobilization, determined by HPLC, was confirmed to be in the effective order. Results from the culture of rat osteosarcoma (ROS), osteoblastic-like cells, demonstrate that the immobilization of RGDS could effectively enhance the attachment of ROS cells on PLLA and increase the cell density in PLLA scaffolds. In addition, experiments of in vitro mineralization indicate that there were more cells and mineralization focci in the RGDS-immobilized scaffolds, suggesting a tendency to form bone-like tissues, compared with the unmodified PLLA scaffold. On the other hand, the PLLA scaffolds immobilized with RGES (Arg-Gly-Glu-Ser) were much less effective in promotion of ROS attachment, suggesting that the enhancement on cell attachment was mainly due to the recognition of RGDS by the adhesion receptors on the cell membrane. The results presented in this work demonstrate that RGDS could be successfully immobilized on PLLA scaffolds with plasma treatment and such modification can make PLLA scaffolds more suitable for culture of osteoblast-like cells and for generation of bone-like tissues.
Ho
Ming-Hua
MH
Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
Hou
Lein-Tuan
LT
Tu
Chen-Yuan
CY
Hsieh
Hsyue-Jen
HJ
Lai
Juin-Yih
JY
Chen
Wei-Jung
WJ
Wang
Da-Ming
DM
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D002113:Calcification, Physiologic / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000737:chemistry*; D010949:Plasma / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D009842:Oligopeptides; D011091:Polyesters; C033616:poly(lactide); C046617:arginyl-glycyl-aspartyl-serine