title: Ewald sphere correction for single-particle electron microscopy.
abstract: Most algorithms for three-dimensional (3D) reconstruction from electron micrographs assume that images correspond to projections of the 3D structure. This approximation limits the attainable resolution of the reconstruction when the dimensions of the structure exceed the depth of field of the microscope. We have developed two methods to calculate a reconstruction that corrects for the depth of field. Either method applied to synthetic data representing a large virus yields a higher resolution reconstruction than a method lacking this correction.
authors.affiliation: Rosenstiel Basic Medical Sciences Research Center, Brandeis University, 415 South Street, Waltham, MA 02454, USA.
authors.forename: Matthias
authors.initials: M
authors.lastname: Wolf
authors.forename: David J
authors.initials: DJ
authors.lastname: DeRosier
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Grigorieff
mesh_terms: D000465:Algorithms; D003626:Data Display; D005583:Fourier Analysis; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D014780:Viruses / Q000737:chemistry / Q000648:ultrastructure