Decreased susceptibility to nitroimidazoles among Bacteroides species in Brazil.
In this study, 197 strains of Bacteroides genus from different species and origins were evaluated with regard to their susceptibility to 5-nitroimidazoles (5-Ni)-such as tinidazole, ornidazole, and metronidazole-using the agar dilution method. The presence of nim genes was also investigated by polymerase chain reaction. It was found that 5.6% of Bacteroides strains among all origins showed decreased susceptibility (minimum inhibitory concentrations varying from 4 to 16 microg/ml) to at least one of the imidazoles studied without any known nim gene associate. Also, we detected one strain isolated from a polluted aquatic environment in which one nim gene was found and characterized as nim B using restriction fragment length polymorphism and sequencing. Hence, resistance to 5-Ni should be monitored closely because they constitute, among few drugs, the ones quite effective in treating Bacteroides infections.
B D Vieira
Jessica Manya
JM
Laboratório de Biologia de Anaeróbios, Departamento de Microbiologia Médica, Instituto de Microbiologia Professor Paulo de Góes, CCS, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. jmanya@terra.com.br
Boente
Renata F
RF
Rodrigues Miranda
Karla
K
Avelar
Kátia E S
KE
M C P Domingues
Regina
R
Candida de S Ferreira
Maria
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001439:Bacteroides / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D001442:Bacteroides Infections / Q000382:microbiology; D001483:Base Sequence; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009593:Nitroimidazoles / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D009593:Nitroimidazoles