title: Nanoparticle-mediated local and remote manipulation of protein aggregation.
abstract: The local heat delivered by metallic nanoparticles selectively attached to their target can be used as a molecular surgery to safely remove toxic and clogging aggregates. We apply this principle to protein aggregates, in particular to the amyloid beta protein (Abeta) involved in Alzheimer's disease (AD), a neurodegenerative disease where unnaturally folded Abeta proteins self-assemble and deposit forming amyloid fibrils and plaques. We show the possibility to remotely redissolve these deposits and to interfere with their growth, using the local heat dissipated by gold nanoparticles (AuNP) selectively attached to the aggregates and irradiated with low gigahertz electromagnetic fields. Simultaneous tagging and manipulation by AuNP of Abeta at different stages of aggregation allow both, noninvasive exploration and dissolution of molecular aggregates.
authors.affiliation: Institut de Recerca Biomèdica de Barcelona, Parc Científic de Barcelona-UB, Spain. mkogan@ciq.uchile.cl
authors.forename: Marcelo J
authors.initials: MJ
authors.lastname: Kogan
authors.forename: Neus G
authors.initials: NG
authors.lastname: Bastus
authors.forename: Roger
authors.initials: R
authors.lastname: Amigo
authors.forename: Dolors
authors.initials: D
authors.lastname: Grillo-Bosch
authors.forename: Eyleen
authors.initials: E
authors.lastname: Araya
authors.forename: Antonio
authors.initials: A
authors.lastname: Turiel
authors.forename: Amilcar
authors.initials: A
authors.lastname: Labarta
authors.forename: Ernest
authors.initials: E
authors.lastname: Giralt
authors.forename: Victor F
authors.initials: VF
authors.lastname: Puntes
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000528:radiation effects; D019281:Dimerization; D004574:Electromagnetic Fields; D017979:Gold Colloid / Q000737:chemistry* / Q000528:radiation effects; D006361:Heating; D046529:Microscopy, Electron, Transmission; D008872:Microwaves; D049329:Nanostructures; D010446:Peptide Fragments / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D016229:Amyloid beta-Peptides; D017979:Gold Colloid; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)