title: [Study on the burden of abnormal hematopoietic clone of the patients with myelodysplastic syndromes and its implications].
abstract: OBJECTIVE
  To investigate the abnormal hematopoietic clone burden of the patients with myelodysplastic syndromes (MDS) and its clinical implication.
  
  
  METHODS
  The ratio of the metaphase with abnormal karyotypes to the total was regarded as the index of MDS clonal burden. Thirteen parameters were assayed and the correlations between these parameters and MDS clone burden were analysed.
  
  
  RESULTS
  The clonal burden of MDS patients was (67.4 +/- 36.2)%. It correlated positively with bone marrow blasts (r = 0.483, P < 0.05), negatively with hemoglobin level (r = -0.445, P < 0.05). The number of blasts, hemoglobin and erythrocytes in high clonal burden (>50%) and low clonal burden (< or = 50%) groups were significantly different (P < 0.05). CD4+ T lymphocytes of MDS patients and normal controls were (274.18 +/-71.85) x 10(6)/L and (454.82 +/- 205.88) x 10(6)/L (P < 0.05) respectively. CD8+ T lymphocytes between MDS patients and normal controls had no difference. The serum level of IL-2 of MDS patients and normal control groups were (6.29 +/- 3.58) g/L and (3.11 +/- 1.40) microg/L (P < 0.05) respectively; but no difference in the serum level of TNF between MDS and control groups. The ratio of CD4+ to CD8+ in high clonal burden patients was 1.90 + 0.52, and in low clonal burden patients was 0.97 +/- 0.44 (P < 0.05).
  
  
  CONCLUSIONS
  The clonal burden and deficient T cell immunity are the indicators for predicting MDS patients clinical progression.
authors.affiliation: Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin, China.
authors.forename: Hua-Quan
authors.initials: HQ
authors.lastname: Wang
authors.forename: Zong-Hong
authors.initials: ZH
authors.lastname: Shao
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
authors.forename: Yan-Ran
authors.initials: YR
authors.lastname: Cao
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.forename: Mei-Feng
authors.initials: MF
authors.lastname: Tu
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Xing
authors.forename: Zhen-Zhu
authors.initials: ZZ
authors.lastname: Cui
authors.forename: Shi-He
authors.initials: SH
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Sun
authors.forename: Hai-Rong
authors.initials: HR
authors.lastname: Jia
authors.forename: Tian-Ying
authors.initials: TY
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000473:pathology*; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology