Immobilization of cells with surface-displayed chitin-binding domain.
To explore chitin-binding domain (ChBD)-based cell immobilization, a tripartite gene fusion consisting of an in-frame fusion of ChBD to lpp and ompA was constructed and expressed in Escherichia coli. ChBD-displayed cells exhibited highly specific and stable binding to chitin within a wide range of pHs (5 to 8) and temperatures (15 to 37 degrees C). These results illustrate the promising use of this approach for engineering applications.
Wang
Jen-You
JY
Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, Taichung, Taiwan.
Chao
Yun-Peng
YP
D000327:Adsorption; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D001709:Biotechnology / Q000379:methods; D018914:Cells, Immobilized; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; C099932:Lpp protein, E coli; D011993:Recombinant Fusion Proteins; D002686:Chitin; C078167:OMPA outer membrane proteins; D002688:Chitinases