Induction of RhoGAP and pathological changes characteristic of Alzheimer's disease by UAHFEMF discharge in rat brain.
Novel experiments with Ultrasound Associated with High Frequency Electromagnetic Field (UAHFEMF) irradiation on rats and mice found evidences of characteristic Alzheimer's disease (AD) degenerations including neurite plaques, beta-amyloid, TAU plaque and deposition in cells, Neuro-Fibrillary Tangle and Paired Helical Filament (PHF) with rats and mice irradiated up to 2454 hours. Concomitant passive avoidance test was performed on six mice, and all showed signs of visual and auditory agnosia and lost cognition of threatening condition. The post section Thioflavin-S fluorescent microscopy found dilated ventricles and dense amyloid-deposition in Ca3 and dentate gyrus. In addition, PHF was identified in the 2454 hours-irradiated rat brain by electron microscope. A human T-cell activation RhoGTPase-activating protein (TAGAP) isoform b homolog (GenBank accession # P84107) induced in the UAHFEMF-treated rat brain was identified using electron spray ionization (ESI) liquid chromatography tandem mass spectrometry (LC/MS/MS). We hypothesized that one of the causes of AD can be the UAHFEMF discharges in human brain.
Chang
Ing-Feng
IF
Department of Biochemistry, University of Nevada, Reno, 89557, USA.
Hsiao
Huo-Yen
HY
D000544:Alzheimer Disease / Q000209:etiology* / Q000378:metabolism / Q000473:pathology*; D000686:Amyloidosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D000818:Animals; D004574:Electromagnetic Fields / Q000009:adverse effects*; D020690:GTPase-Activating Proteins / Q000737:chemistry / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009410:Nerve Degeneration / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D016874:Neurofibrillary Tangles / Q000473:pathology / Q000528:radiation effects; D009474:Neurons / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D058225:Plaque, Amyloid / Q000473:pathology / Q000528:radiation effects; D010949:Plasma / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D016875:tau Proteins / Q000378:metabolism
D020690:GTPase-Activating Proteins; D016875:tau Proteins; D006861:Hydrogen Peroxide