Growth inhibition by nitrocompounds of selected uric acid-utilizing microorganisms isolated from poultry manure.
The overall objective of this study was to evaluate the potential ability of nitrocompounds to reduce ammonia volatilization by inhibiting uric acid-utilizing microorganisms. Experiment I was conducted to evaluate the effects of nitrocompounds on the growth of uric acid-utilizing microorganisms isolated from poultry manure during six-hour incubation. There were five treatments: (1) control, (2) 50 mM nitroethane, (3) 50 mM nitroethanol, (4) 50 mM nitropropanol, and (5) 50 mM nitropropionic acid. Optical density values of nitrocompounds were significantly lower than that of control at two, four, and six hours. Plate counts of uric acid-utilizing microorganisms after six-hour incubation exhibited that nitrocompounds greatly reduced the growth of these microorganisms except for the nitroethane (P < 0.05). The nitropropanol and nitropropionic acid treatments showed significantly higher inhibitory effects compared to the nitroethanol. Experiments II and III were conducted to evaluate inhibitory effects of nitrocompounds on growth of uric acid-utilizing microorganisms compared to non-nitrocompounds such as ethanol, propanol, and propionic acid. Experiments II and III consisted of seven treatments: (1) control, (2) nitroethanol, (3) nitropropanol, (4) nitropropionic acid, (5) ethanol, (6) propanol, and (7) propionic acid. The incubation times of Experiments II and III were 6 and 24 h, respectively. The nitrocompounds were significantly more successful in inhibiting growth of uric acid-utilizing microorganisms compared to those non-nitrocompounds. These results suggest that nitrocompounds exhibit potential to reduce ammonia volatilization in poultry manure by inhibiting growth of uric acid-utilizing microorganisms.
Kim
W K
WK
Department of Poultry Science, Texas A&M University, College Station, Texas 77843-2377, USA.
Anderson
R C
RC
Ratliff
A L
AL
Nisbet
D J
DJ
Ricke
S C
SC
D000393:Air Pollutants / Q000032:analysis; D000641:Ammonia / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D015169:Colony Count, Microbial; D008372:Manure / Q000032:analysis / Q000382:microbiology*; D017672:Nitrogen Compounds / Q000494:pharmacology*; D011200:Poultry; D012037:Refuse Disposal; D013997:Time Factors; D014527:Uric Acid / Q000378:metabolism*; D014835:Volatilization
D000393:Air Pollutants; D008372:Manure; D017672:Nitrogen Compounds; D014527:Uric Acid; D000641:Ammonia