Identification and characterization of peptides binding to newcastle disease virus by phage display.
Three individual peptide sequences, EVSHPKVG, WVTTSNQW, and SGGSNRSP, which have potentials to bind to Newcastle disease virus (NDV), were identified by the biopanning method using phage display technology. The binding specificities of these peptides presented on phages were confirmed by ELISA competition assay using chicken anti-NDV antiserum. The synthetic peptides designed based on these results partially neutralized the infection of NDV in vitro. The peptide-motives identified here have the potential to lead to the identification of novel molecules that inhibit the NDV infection independent of the immune system.
Ozawa
Makoto
M
Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Ohashi
Kazuhiko
K
Onuma
Misao
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009522:Newcastle disease virus / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D019151:Peptide Library; D010455:Peptides