title: A variable region in the Crimean-Congo hemorrhagic fever virus L segment distinguishes between strains isolated from different geographic regions.
abstract: Alignment of Crimean-Congo hemorrhagic fever virus (CCHFV) L genome segment full-length sequences reveals an overall high level of conservation among strains, with greater than 90% of translated amino acid residues strictly conserved. However, a region of marked variability identified previously, corresponding to L polyprotein amino acid positions 760-810, shares only 40% overall identity between strains. The variable regions sequences of 16 laboratory-adapted CCHFV strains were determined, including 11 strains from European Russia, one strain from Bulgaria, and four strains from the Central Asian countries of Tajikistan, Turkmenistan, and Uzbekistan. Phylogenetic analysis demonstrates this L segment variable region sequence divides CCHFV strains into similar geographically-defined groupings observed for S segment-derived trees, but with higher bootstrap support and a much smaller character set required for analysis.
authors.affiliation: Department of Microbiology, University of New Mexico, Albuquerque, New Mexico, USA.
authors.forename: John D
authors.initials: JD
authors.lastname: Meissner
authors.forename: Sergei S
authors.initials: SS
authors.lastname: Seregin
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Seregin
authors.forename: Oleg I
authors.initials: OI
authors.lastname: Vyshemirskii
authors.forename: Evgeny I
authors.initials: EI
authors.lastname: Samokhvalov
authors.forename: Dmiytrii K
authors.initials: DK
authors.lastname: Lvov
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Netesov
authors.forename: Vladimir S
authors.initials: VS
authors.lastname: Petrov
mesh_terms: D000349:Africa; D000595:Amino Acid Sequence; D000818:Animals; D001208:Asia; D005060:Europe; D014644:Genetic Variation; D016679:Genome, Viral; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013045:Species Specificity; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins