Transgenic tobacco plants expressing BoRS1 gene from Brassica oleracea var. acephala show enhanced tolerance to water stress.
Water stress is by far the leading environmental stress limiting crop yields worldwide. Genetic engineering techniques hold great promise for developing crop cultivars with high tolerance to water stress. In this study, the Brassica oleracea var. acephala BoRS1 gene was transferred into tobacco through Agrobacterium-mediated leaf disc transformation. The transgenic status and transgene expression of the transgenic plants was confirmed by polymerase chain reaction (PCR) analysis, Southern hybridization and semi-quantitative one step RT-PCR analysis respectively. Subsequently, the growth status under water stress, and physiological responses to water stress of transgenic tobacco were studied. The results showed that the transgenic plants exhibited better growth status under water stress condition compared to the untransformed control plants. In physiological assessment of water tolerance, transgenic plants showed more dry matter accumulation and maintained significantly higher levels of leaf chlorophyll content along with increasing levels of water stress than the untransformed control plants. This study shows that BoRS1 is a candidate gene in the engineering of crops for enhanced water stress tolerance.
Tang
Dongqin
D
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 201101, People's Republic of China.
Qian
Hongmei
H
Zhao
Lingxia
L
Huang
Danfeng
D
Tang
Kexuan
K
D016960:Agrobacterium tumefaciens / Q000235:genetics; D001681:Biological Assay; D015139:Blotting, Southern / Q000379:methods; D001937:Brassica / Q000235:genetics; D002734:Chlorophyll / Q000032:analysis; D003681:Dehydration / Q000235:genetics; D018525:Germination / Q000502:physiology; D006360:Heat-Shock Proteins / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D014170:Transformation, Genetic
D006360:Heat-Shock Proteins; D010940:Plant Proteins; D002734:Chlorophyll