Dicer and positive charge of proteins decrease the stability of RNA containing the AU-rich element of GM-CSF.
AU-rich elements (AREs) in the 3'-untranslated region of mRNAs promote rapid decay of the mRNAs for certain cytokines, including that encoding granulocyte-macrophage colony-stimulating factor (GM-CSF). We show that an RNA molecule based on the ARE of GM-CSF mRNA is cleaved between U and A residues in the presence of bovine serum albumin of which cleavage effect is attenuated by acetylation. Furthermore, the expression of RNA molecule containing the ARE of GM-CSF mRNA in human cell lines was increased by inhibition of histone deacetylase activity and attenuation of Dicer expression. These findings suggest that degradation of mRNAs containing an ARE might be regulated by positive charge of polypeptides and Dicer.
Takahashi
Hidehiro
H
Department of Pathology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan. htakahas@nih.go.jp
Maeda
Masae
M
Sawa
Hirofumi
H
Hasegawa
Hideki
H
Moriyama
Masami
M
Sata
Tetsutaro
T
Hall
William W
WW
Kurata
Takeshi
T
D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D053487:DEAD-box RNA Helicases; D004722:Endoribonucleases / Q000737:chemistry / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D008156:Luciferases / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry*; D020365:RNA Helicases / Q000737:chemistry / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043244:Ribonuclease III / Q000737:chemistry / Q000502:physiology*; D012709:Serum Albumin / Q000378:metabolism; D013997:Time Factors
D020413:3' Untranslated Regions; D016207:Cytokines; D006657:Histones; D010455:Peptides; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D012709:Serum Albumin; D012313:RNA; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D008156:Luciferases; D004722:Endoribonucleases; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases