The endophytic fungus Trametes hirsuta as a novel alternative source of podophyllotoxin and related aryl tetralin lignans.
The aryl tetralin lignans are synthesized by Podophyllum sps. and are in great demand worldwide due to their use in synthesis of topoisomerase inhibitors. However, the sustained production of these aryl tetralin lignans requires large-scale harvesting from the natural environments, which has resulted in the plant-endangered status. In view of the difficulties in their total chemical synthesis, cultivation and failure of metabolic engineering approaches, there is a need to search for alternative sources of production of aryl tetralin lignans. We unequivocally established the methodology for isolation, identification, and characterization of a novel fungal endophyte (Trametes hirsuta) that produces aryl tetralin lignans consistently as shown by HPLC, LC-MS, LC/MS-MS and (1)H NMR. The lignans produced by the microorganism are biologically active, and exhibit potent antioxidant, anticancer and radioprotective properties. This strategy promises to improve the production of these therapeutically important compounds at lower costs.
Puri
Satish Chandra
SC
Natural Products Chemistry Division, Regional Research Laboratory (CSIR), Jammu 180001, India. rks@inmas.org
Nazir
Asiya
A
Chawla
Raman
R
Arora
Rajesh
R
Riyaz-Ul-Hasan
S
S
Amna
Touseef
T
Ahmed
Bilal
B
Verma
Vijeshwar
V
Singh
Shikha
S
Sagar
Ravinder
R
Sharma
Ashok
A
Kumar
Raj
R
Sharma
Rakesh Kumar
RK
Qazi
Ghulam Nabi
GN
D002458:Cell Fractionation; D002851:Chromatography, High Pressure Liquid; D005658:Fungi / Q000166:cytology / Q000302:isolation & purification*; D007700:Kinetics; D017705:Lignans / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000528:radiation effects; D008954:Models, Biological; D059828:Plant Cells; D018514:Plant Structures / Q000166:cytology / Q000378:metabolism; D010944:Plants / Q000378:metabolism; D011034:Podophyllotoxin / Q000737:chemistry* / Q000378:metabolism; D011035:Podophyllum / Q000166:cytology / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013764:Tetrahydronaphthalenes / Q000737:chemistry*
D017705:Lignans; D013764:Tetrahydronaphthalenes; C095210:tetralin; D011034:Podophyllotoxin