title: Identification of vaccine candidate antigens of an ESBL producing Klebsiella pneumoniae clinical strain by immunoproteome analysis.
abstract: Klebsiella pneumoniae is an opportunistic pathogen which causes pneumoniae, urinary tract infections and septicemia in immunocompromised patients. Hospital outbreaks of multidrug-resistant K. pneumoniae, especially those in neonatal wards, are often caused by strains producing the extended-spectrum-beta-lactamases (ESBLs). An immunoproteome based approach was developed to identify candidate antigens of K. pneumoniae for vaccine development. Sera from patients with acute K. pneumoniae infections (n = 55) and a control group of sera from healthy individuals (n = 15) were analyzed for reactivity by Western blot against ESBL K. pneumoniae outer membrane proteins separated by 2-DE. Twenty highly immunogenic protein spots were identified by immunoproteomic analysis. The immunogenic proteins that are most frequently recognized by positive K. pneumoniae sera were OmpA, OmpK36, FepA, OmpK17, OmpW, Colicin I receptor protein and three novel proteins. Two of the vaccine candidate genes, OmpA (Struve et al. Microbiology 2003, 149, 167-176) and FepA (Lai, Y. C. et al.. Infect Immun 2001, 69, 7140-7145), have recently been shown to be essential in colonization and infection in an in vivo mouse model. Hence, these two immunogenic proteins could serve as potential vaccine candidates.
authors.affiliation: Department of Microbiology, National University of Singapore, Singapore.
authors.forename: Prathiba
authors.initials: P
authors.lastname: Kurupati
authors.forename: Boon King
authors.initials: BK
authors.lastname: Teh
authors.forename: Gamini
authors.initials: G
authors.lastname: Kumarasinghe
authors.forename: Chit Laa
authors.initials: CL
authors.lastname: Poh
mesh_terms: D000208:Acute Disease; D000942:Antigens, Bacterial / Q000032:analysis*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001428:Bacterial Vaccines; D015153:Blotting, Western; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007710:Klebsiella Infections / Q000097:blood; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000276:immunology*; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D020543:Proteome; C117199:beta-lactamase TEM-61; D001618:beta-Lactamases