[Screening of microorganisms--producers of alpha-L-rhamnosidase].
Screening of producers of alpha-L-rhamnosidase among 692 strains of microorganisms of different taxonomic groups has been performed. A capacity to synthesize the enzyme was revealed in 35.7% of the studied cultures. Representatives of genera Aspergillus, Myrothecium, Penicillium, Eupenicillium (activity 0.1 - 0.335 un./mg of protein) proved to be the most active producers. Complex preparations of alpha-L-rhamnosidase were obtained from cultural liquid of 10 producers by fractionation with ammonium sulfate (30 and 90% saturation); pH- and thermal optimum, as well as pH- and thermostability were analyzed in 7 of them. The Aspergillus, Penicillium, Eupenicillium strains which display high stability and activity under technological values of temperature 20-37 degrees C and medium pH 4.0-6.0 have been chosen for further investigations.
Rzaieva
O M
OM
Borzova
N V
NV
Varbanets'
L D
LD
Sokolova
O V
OV
Kharkevych
O S
OS
Zhdanova
N M
NM
Safronova
L A
LA
D000645:Ammonium Sulfate; D001407:Bacillus / Q000145:classification / Q000201:enzymology*; D011232:Chemical Precipitation; D004355:Drug Stability; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D003904:Mitosporic Fungi / Q000145:classification / Q000201:enzymology*; D013045:Species Specificity; D013696:Temperature
D006026:Glycoside Hydrolases; C022525:alpha-L-rhamnosidase; D000645:Ammonium Sulfate