Influence of biosurfactant on interactive forces between mutans Streptococci and enamel measured by atomic force microscopy.
Although interactive forces, influenced by environmental conditions, between oral bacteria and tooth surfaces are important for the development of plaque, they have never been estimated. It is hypothesized that interactive forces, as measured by atomic force microscopy, between enamel with or without a pellicle and two strains of mutans streptococci become less attractive by the application of a Streptococcus mitis BMS biosurfactant coating. Upon approach of each of the strains toward bare and pellicle-coated enamel, adsorbed biosurfactant increased the range of the repulsive forces. Upon retraction of the enamel surface, small adhesion forces (0.8-0.9 nN) were measured for bare enamel that almost disappeared after biosurfactant coating. The prevalence and magnitude of the adhesion forces also decreased upon pellicle-coating of the enamel, with a minor effect of adsorbed biosurfactant. These findings indicate that adsorbed S. mitis BMS biosurfactant changes the interactive forces between the mutans streptococci studied and enamel, explaining the effects of biosurfactant on adhesion.
van Hoogmoed
C G
CG
Department of Biomedical Engineering, University Medical Centre Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands. C.G.van.Hoogmoed@med.rug.nl
Dijkstra
R J B
RJ
van der Mei
H C
HC
Busscher
H J
HJ
D000327:Adsorption; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D001696:Biomechanical Phenomena; D002417:Cattle; D003743:Dental Enamel / Q000382:microbiology* / Q000648:ultrastructure; D044622:Dental Pellicle / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D034361:Streptococcus mitis; D013295:Streptococcus mutans / Q000187:drug effects*; D016986:Streptococcus sobrinus / Q000187:drug effects*; D013500:Surface Tension; D013501:Surface-Active Agents / Q000494:pharmacology*
D013501:Surface-Active Agents