LOCATE: a mouse protein subcellular localization database.
We present here LOCATE, a curated, web-accessible database that houses data describing the membrane organization and subcellular localization of proteins from the FANTOM3 Isoform Protein Sequence set. Membrane organization is predicted by the high-throughput, computational pipeline MemO. The subcellular locations of selected proteins from this set were determined by a high-throughput, immunofluorescence-based assay and by manually reviewing >1700 peer-reviewed publications. LOCATE represents the first effort to catalogue the experimentally verified subcellular location and membrane organization of mammalian proteins using a high-throughput approach and provides localization data for approximately 40% of the mouse proteome. It is available at http://locate.imb.uq.edu.au.
Fink
J Lynn
JL
ARC Centre in Bioinformatics, University of Queensland, St Lucia, Queensland 4072, Australia. l.fink@imb.uq.edu.au
Aturaliya
Rajith N
RN
Davis
Melissa J
MJ
Zhang
Fasheng
F
Hanson
Kelly
K
Teasdale
Melvena S
MS
Kai
Chikatoshi
C
Kawai
Jun
J
Carninci
Piero
P
Hayashizaki
Yoshihide
Y
Teasdale
Rohan D
RD
D000818:Animals; D030562:Databases, Protein; D020407:Internet; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D014584:User-Computer Interface
D008565:Membrane Proteins; D020033:Protein Isoforms; D020543:Proteome