title: Determination of human complement factor C4 using resonance light-scattering technique with sodium dodecylbenzene sulphonate probe.
abstract: Based on the interaction between human complement factor C4 (human C4) and sodium dodecylbenzene sulphonate (SDBS) and the resonance light-scattering (RLS) technique, a highly sensitive assay for human C4 using resonance light-scattering technique was developed. At pH 2.8 Na2HPO4-citric acid buffer solution, the RLS intensities of SDBS system at 283, 503 and 600 nm were obviously enhanced in the presence of human C4. The effects of surfactant, pH, incubation time, concentration of SDBS and foreign substances on the enhanced RLS intensity of system were investigated. Under the optimum conditions, the enhanced RLS intensity is directly proportional to the concentration of human C4 in the range of (0.5-120)x10(-6)gl-1 and the linear regression equation was obtained with high correlation coefficient. This RLS technique was applied to the determination of human C4 in some synthetic samples with good recovery. Moreover, it was found that the electrostatic interaction is the main binding force between SDBS and human C4.
authors.affiliation: College of Chemistry, Jilin University, Linyuan Road 1788, Changchun 130012, China.
authors.forename: Dejiang
authors.initials: D
authors.lastname: Gao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tian
authors.forename: Lan
authors.initials: L
authors.lastname: Ding
authors.forename: Fanghui
authors.initials: F
authors.lastname: Liang
authors.forename: Shuyun
authors.initials: S
authors.lastname: Bi
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Chen
authors.forename: Aimin
authors.initials: A
authors.lastname: Yu
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
mesh_terms: D001557:Benzenesulfonates / Q000737:chemistry*; D003181:Complement C4 / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry; D013997:Time Factors
chemical_list: D001557:Benzenesulfonates; D003181:Complement C4; D013501:Surface-Active Agents; C001114:dodecylbenzenesulfonic acid