Bilirubin oxidase activity of Bacillus subtilis CotA.
The spore coat protein CotA from Bacillus subtilis was previously identified as a laccase. We have now found that CotA also shows strong bilirubin oxidase activity and markedly higher affinity for bilirubin than conventional bilirubin oxidase. This is the first characterization of bilirubin oxidase activity in a bacterial protein.
Sakasegawa
Shin-ichi
S
Asahi Kasei Pharma Corporation, Shizuoka 410-2321, Japan. sakasegawa.sb@om.asahi-kasei.co.jp.
Ishikawa
Hidehiko
H
Imamura
Shigeyuki
S
Sakuraba
Haruhiko
H
Goda
Shuichiro
S
Ohshima
Toshihisa
T
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000502:physiology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001663:Bilirubin / Q000097:blood; D004795:Enzyme Stability; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042845:Laccase; D008969:Molecular Sequence Data; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000378:metabolism*; D013171:Spores, Bacterial / Q000737:chemistry
D001426:Bacterial Proteins; D042845:Laccase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D001663:Bilirubin