Role of L-histidine in conferring tolerance to Ni2+ in Sacchromyces cerevisiae cells.
Ni(2+) toxicity can be alleviated in yeast cells by exogenous L-histidine, but not by its enantiomer, D-histidine, nor by other natural L-amino acids tested. We studied the effect of L-histidine upon the accumulation and intracellular distribution of Ni(2+) and found that moderate L-histidine concentrations (less than or equal to those of Ni(2+)) increased cell tolerance without decreasing Ni(2+) accumulation. Although excess L-histidine appeared to lower Ni(2+) accumulation, the concomitant presence of Ni(2+) and L-histidine in the growth medium stimulated each other's uptake.
Farcasanu
Ileana Cornelia
IC
Department of Organic Chemistry and Biochemistry, Faculty of Chemistry, Bucharest University, Romania.
Mizunuma
Masaki
M
Nishiyama
Fumitaka
F
Miyakawa
Tokichi
T
D000596:Amino Acids / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D003470:Culture Media; D006639:Histidine / Q000494:pharmacology*; D009532:Nickel / Q000378:metabolism / Q000633:toxicity*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism
D000596:Amino Acids; D002614:Chelating Agents; D003470:Culture Media; D006639:Histidine; D009532:Nickel