Inhibition of preadipocyte differentiation by germacranolides from Calea urticifolia in 3T3-L1 cells.
The effects of germacranolides isolated from Calea urticifolia on adipocytic differentiation of 3T3-L1 cells were examined. These germacranolides inhibited adipogenesis at a concentration of 1.25-5 microM. But no inhibitory activity against cell proliferation and no nonspecific binding activity to protein were observed. These results indicate that these germacranolides are the specific inhibitors of preadipocyte differentiation.
Matsuura
Nobuyasu
N
Department of Life Science, Faculty of Science, Okayama University of Science, Japan. nobuyasu@dis.ous.ac.jp
Yamada
Masashi
M
Suzuki
Hiroto
H
Hasegawa
Naoko
N
Kurosaka
Chihiro
C
Ubukata
Makoto
M
Tanaka
Toshiyuki
T
Iinuma
Munekazu
M
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects*; D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D050356:Lipid Metabolism; D051379:Mice; D045788:Sesquiterpenes, Germacrane / Q000494:pharmacology*; D013234:Stem Cells / Q000187:drug effects*
D045788:Sesquiterpenes, Germacrane; C432449:germacranolide