title: [Effect of Huchang Qingfei concentrated pellets on E-cadherin expression in lung tissue of mice infected with Mycoplasma pneumoniae].
abstract: OBJECTIVE
  To investigate the effects of Huchang Qingfei concentrated pellets on the expression of E-cadherin (E-cd) in the lung tissue from mice infected with Mycoplasma pneumoniae (MP).
  
  
  METHODS
  A mice model of Mycoplasmal pneumonia (MPP) was developed by repeatedly intranasal infectious route. Transmission electronic microscope (TEM) and immunohistochemistry stain were performed to observe the pathological changes and expression of E-cd in lung tissues.
  
  
  RESULTS
  Under TEM it was found that the cellular membrane was ruptured, mitochondria was denatured, crista was broken in the pulmonary cells of the model group; the all above parameters in Huchang medicated group were improved obviously. The immunohistochemistry test showed that strong positive brown stain of E-cd expression was found in the pulmonary epithelial cell membrane and bronchial periphery in the model group, however, in the medicated group, the E-cd expression level in the cellular membrane was decreased and the expression ratio was dropped significantly as compared with the model controls.
  
  
  CONCLUSIONS
  Huchang Qingfei concentrated pellets can inhibit the overexpression of E-cd in the lung tissue of mice with MP-infection, which may be helpful for prevention and treatment of pulmonary injury caused by MPP.
authors.affiliation: Hei Longjiang Academy of Traditional Chinese Medicine Research Institute, Harbin 150036, China.
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Wang
authors.forename: Ji-chang
authors.initials: JC
authors.lastname: Li
authors.forename: Hong-juan
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Gui-ying
authors.initials: GY
authors.lastname: Yang
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry; D011019:Pneumonia, Mycoplasma / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D011897:Random Allocation
chemical_list: D015820:Cadherins; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal