title: Capillary electrophoretic analysis of the antibiotic vancomycin in innovative microparticles and in commercial formulations.
abstract: A new fast capillary electrophoretic method has been developed for the analysis of the glycopeptide antibiotic vancomycin in formulations. An electrophoretic run is completed within 3.0 min; fused silica capillaries (100 microm i.d., 8.5 cm effective length and 48.5 cm total length) and a background electrolyte consisting of 12.5 mM, pH 2.5 phosphate buffer are used. The applied voltage is -20.0 kV; samples are injected by pressure (30 mbar x 3 s) at the anodic end of the capillary. The method was successfully applied to innovative controlled release microparticles consisting of a coated albumin core containing vancomycin. A simple procedure has been developed to obtain complete vancomycin extraction from microparticles using a 5% (w/v) sodium dodecyl sulphate aqueous solution. The method has been validated in terms of linearity, precision and accuracy. Good linearity was found in the 0.25-5.00 microg/mL range. Satisfactory precision was obtained, with relative standard deviation values always lower than 3.9%; accuracy was satisfactory, with recovery values between 97.8 and 102.2%. The method is also suitable for vancomycin determination in commercial capsules.
authors.affiliation: Faculty of Pharmacy, Department of Pharmaceutical Sciences, Alma Mater Studiorum-University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Musenga
authors.forename: R
authors.initials: R
authors.lastname: Mandrioli
authors.forename: V
authors.initials: V
authors.lastname: Zecchi
authors.forename: B
authors.initials: B
authors.lastname: Luppi
authors.forename: S
authors.initials: S
authors.lastname: Fanali
authors.forename: M A
authors.initials: MA
authors.lastname: Raggi
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002214:Capsules; D002626:Chemistry, Pharmaceutical; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D014640:Vancomycin / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D002214:Capsules; D014640:Vancomycin