Neurite outgrowth promoting effects of enriched and mixed OEC/ONF cultures.
Olfactory ensheathing cell (OEC) transplants stimulate axon regeneration and partial functional recovery after spinal cord injury. However, it remains unclear whether enriched OEC or mixed transplants of OEC and olfactory nerve fibroblasts (ONF) are optimal for stimulating axon regrowth. The neurite outgrowth stimulating effects of enriched OEC, ONF, and mixed OEC/ONF cultures on neonatal cerebral cortical neurons were compared using co-cultures. We show that (1) OEC are more neurite outgrowth promoting than ONF, and (2) ONF do not enhance the neurite outgrowth stimulating effects of OEC in mixed OEC/ONF cultures. Hence, our data indicate that there is no preference for the use of enriched OEC or mixed OEC/ONF cultures with respect to stimulation of neurite growth in vitro.
Deumens
Ronald
R
Department of Psychiatry and Neuropsychology, Division Neuroscience, European Graduate School of Neuroscience (EURON), Maastricht University, P.O. Box 616, 6200 MD, Maastricht, The Netherlands. r.deumens@np.unimaas.nl
Koopmans
Guido C
GC
Lemmens
Marijke
M
Möllers
Sven
S
Honig
Wiel M
WM
Steinbusch
Harry W
HW
Brook
Gary
G
Joosten
Elbert A
EA
D000818:Animals; D002452:Cell Count / Q000379:methods; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D018920:Coculture Techniques / Q000379:methods; D005347:Fibroblasts / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000166:cytology*; D009830:Olfactory Bulb / Q000166:cytology*; D009832:Olfactory Nerve / Q000166:cytology*; D051381:Rats; D009418:S100 Proteins / Q000378:metabolism; D013997:Time Factors
C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D009418:S100 Proteins