Detection of recombinant epoetin and darbepoetin alpha after subcutaneous administration in the horse.
A direct detection method for anti-doping control of recombinant human erythropoietin (rHuEPO) abuse in racehorses is proposed. This method involves screening of plasma (or serum) by an enzyme-linked immunosorbent assay specific for human EPO and confirmation in urine samples by characterization of the urinary EPO isoelectric profile. This method was tested on horses that were administered epoetin alpha (rHuEPO) and the hyper-glycosylated form of this drug (darbepoetin alpha).
Lasne
Françoise
F
Laboratoire National de Dépistage du Dopage, Châtenay-Malabry, France. f.lasne@lndd.com
Popot
Marie-Agnes
MA
Varlet-Marie
Emmanuelle
E
Martin
Laurent
L
Martin
Jean-Antoine
JA
Bonnaire
Yves
Y
Audran
Michel
M
de Ceaurriz
Jacques
J
D000818:Animals; D000068256:Darbepoetin alfa; D004797:Enzyme-Linked Immunosorbent Assay; D000068817:Epoetin Alfa; D004921:Erythropoietin / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine; D006736:Horses; D007279:Injections, Subcutaneous; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D004921:Erythropoietin; D000068256:Darbepoetin alfa; D000068817:Epoetin Alfa