title: Correlation between angiotensin-converting enzyme, vascular endothelial growth factor, and matrix metalloproteinase-9 in the vitreous of eyes with diabetic retinopathy.
abstract: OBJECTIVE
  To investigate the involvement of angiotensin-converting enzyme (ACE) with angiogenic factors, vascular endothelial growth factor (VEGF), and matrix metalloproteinase (MMP)-9 in the vitreous of eyes with proliferative diabetic retinopathy (PDR).
  
  
  METHODS
  Observational case series.
  
  
  METHODS
  Angiotensin-converting enzyme activity in the vitreous was measured by using a synthetic substrate for ACE. VEGF and MMP-9 concentrations were determined by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Vitreous ACE activity was significantly higher in eyes with PDR (1.4 +/- 1.3 mU/ml) than in eyes with macular holes (0.22 +/- 0.11 mU/ml). Vitreous VEGF concentration in the eyes with PDR (1067 +/- 1076 pg/ml) was significantly higher than in eyes with macular holes (34 +/- 5.5 pg/ml). Vitreous MMP-9 concentration was also significantly higher in eyes with PDR (7.5 +/- 3.8 ng/ml) than in eyes with macular holes (4.2 +/- 1.4 ng/ml). Significant correlations between ACE and VEGF (P < .01) and between ACE and MMP-9 (P < .01) were observed in the vitreous of eyes with PDR.
  
  
  CONCLUSIONS
  Angiotensin-converting enzyme was significantly correlated with angiogenic factors, VEGF and MMP-9, in the vitreous of eyes with PDR.
authors.affiliation: Department of Ophthalmology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki City, Osaka 569-8686, Japan.
authors.forename: Eisuke
authors.initials: E
authors.lastname: Ishizaki
authors.forename: Shinji
authors.initials: S
authors.lastname: Takai
authors.forename: Mari
authors.initials: M
authors.lastname: Ueki
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Maeno
authors.forename: Midori
authors.initials: M
authors.lastname: Maruichi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Oku
authors.forename: Tsunehiko
authors.initials: T
authors.lastname: Ikeda
authors.forename: Mizuo
authors.initials: M
authors.lastname: Miyazaki
mesh_terms: D000368:Aged; D003930:Diabetic Retinopathy / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000097:blood*; D012167:Retinal Perforations / Q000201:enzymology; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*; D014822:Vitreous Body / Q000201:enzymology*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D007703:Peptidyl-Dipeptidase A; D020780:Matrix Metalloproteinase 9