Novel PCR-restriction fragment length polymorphism analysis for rapid typing of staphylococcal cassette chromosome mec elements.
We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV.
Yang
Jin Ah
JA
Graduate School of Life Science and Biotechnology, College of Medicine, Korea University, Seoul.
Park
Dae Won
DW
Sohn
Jang Wook
JW
Kim
Min Ja
MJ
D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial; D006801:Humans; D008712:Methicillin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D045522:Recombinases / Q000235:genetics*; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics
D001426:Bacterial Proteins; C489156:CcrC protein, Staphylococcus aureus; D045522:Recombinases; D008712:Methicillin