Fabrication of microfluidic devices using dry film photoresist for microchip capillary electrophoresis.
An inexpensive, disposable microfluidic device was fabricated from a dry film photoresist using a combination of photolithographic and hot roll lamination techniques. A microfluidic flow pattern was prefabricated in a dry film photoresist tape using traditional photolithographic methods. This tape became bonded to a poly(methyl methacrylate) (PMMA) sheet with prepouched holes when passed through a hot roll laminator. A copper working electrode and platinum decoupler was readily incorporated within this microchip. The integrated microchip device was then fixed in a laboratory-built Plexiglas holder prior to its use in microchip capillary electrophoresis. The performance of this device with amperometric detection for the separation of dopamine and catechol was examined. The separation was complete within 50 s at an applied potential of 200 V/cm. The relative standard deviations (RSD) of analyte migration times were less than 0.71%, and the theoretical plate numbers for dopamine and catechol were 3.2 x 10(4) and 4.1 x 10(4), respectively, based on a 65 mm separation channel.
Tsai
Yuan-Chien
YC
Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan.
Jen
Hsiu-Ping
HP
Lin
Kuan-Wen
KW
Hsieh
You-Zung
YZ
D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D044085:Microfluidics / Q000295:instrumentation*; D010777:Photochemistry; D019904:Polymethyl Methacrylate
D019904:Polymethyl Methacrylate