Functional characterization of the Bradyrhizobium japonicum modA and modB genes involved in molybdenum transport.
A modABC gene cluster that encodes an ABC-type, high-affinity molybdate transporter from Bradyrhizobium japonicum has been isolated and characterized. B. japonicum modA and modB mutant strains were unable to grow aerobically or anaerobically with nitrate as nitrogen source or as respiratory substrate, respectively, and lacked nitrate reductase activity. The nitrogen-fixing ability of the mod mutants in symbiotic association with soybean plants grown in a Mo-deficient mineral solution was severely impaired. Addition of molybdate to the bacterial growth medium or to the plant mineral solution fully restored the wild-type phenotype. Because the amount of molybdate required for suppression of the mutant phenotype either under free-living or under symbiotic conditions was dependent on sulphate concentration, it is likely that a sulphate transporter is also involved in Mo uptake in B. japonicum. The promoter region of the modABC genes has been characterized by primer extension. Reverse transcription and expression of a transcriptional fusion, P(modA)-lacZ, was detected only in a B. japonicum modA mutant grown in a medium without molybdate supplementation. These findings indicate that transcription of the B. japonicum modABC genes is repressed by molybdate.
Delgado
María J
MJ
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, PO Box 419, 18080 Granada, Spain.
Tresierra-Ayala
Alvaro
A
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, PO Box 419, 18080 Granada, Spain.
Talbi
Chouhra
C
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, PO Box 419, 18080 Granada, Spain.
Bedmar
Eulogio J
EJ
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, PO Box 419, 18080 Granada, Spain.
D001483:Base Sequence; D020369:Bradyrhizobium / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D008982:Molybdenum / Q000378:metabolism*; D050901:Nitrate Reductase / Q000378:metabolism; D009586:Nitrogen Fixation; D009591:Nitrogenase / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D013025:Glycine max / Q000378:metabolism / Q000382:microbiology; D014158:Transcription, Genetic
C044659:molybdate; D008982:Molybdenum; D009591:Nitrogenase; D050901:Nitrate Reductase