Analysis of genetic polymorphism of deoxyribonuclease I in Ovambo and Turk populations using a genotyping method.
Deoxyribonuclease I (DNase I) polymorphism has been used as a valuable marker in genetic and clinical investigations. Six codominant alleles are known for DNase I, DNASE1*1, *2, *3, *4, and the recently discovered alleles *5 and *6. To detect these two new alleles, we added a new DNase I genotyping method based on both an allele-specific amplification and mismatched polymerase chain reaction (PCR). These methods were used to examine the distribution of DNase I genotypes in unrelated individuals from bloodstains of Ovambo and Turkish populations. The DNASE1*1 allele was found to be most dominant in the Ovambos. In contrast, Turks showed the highest allele frequency for DNASE1*2. This study is the first to demonstrate that there is a certain genetic heterogeneity in the worldwide distribution of DNase I polymorphism using the genotyping method of human DNase I polymorphism with PCR.
Fujihara
Junko
J
Department of Legal Medicine, Shimane University School of Medicine, Izumo, Shimane 693-8501, Japan.
Hieda
Yoko
Y
Takayama
Koji
K
Xue
Yuying
Y
Nakagami
Norihito
N
Imamura
Shinji
S
Kataoka
Kaori
K
Takeshita
Haruo
H
D044466:Asian People / Q000235:genetics*; D044383:Black People / Q000235:genetics*; D017931:DNA Primers; D003850:Deoxyribonuclease I / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D009276:Namibia; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D014421:Turkey
D017931:DNA Primers; D003850:Deoxyribonuclease I