Desmin modulates lung elastic recoil and airway responsiveness.
Desmin is a structural protein that is expressed in smooth muscle cells of both airways and alveolar ducts. Therefore, desmin could be well situated to participate in passive and contractile force transmission in the lung. We hypothesized that desmin modulates lung compliance, lung recoil pressure, and airway contractile response. To test this hypothesis, respiratory system complex impedance (Zin,rs) at different positive end-expiratory pressure (PEEP) levels and quasi-static pressure-volume data were obtained in desmin-null and wild-type mice at baseline and during methacholine administration. Airways and lung tissue properties were partitioned by fitting Zin,rs to a constant-phase model. Relative to controls, desmin-null mice showed 1) lower values for lung stiffness and recoil pressure at baseline and induced airway constriction, 2) greater negative PEEP dependence of H and airway resistance under baseline conditions and cholinergic stimulation, and 3) airway hyporesponsiveness. These results demonstrate that desmin is a load-bearing protein that stiffens the airways and consequently the lung and modulates airway contractile response.
Shardonofsky
Felix R
FR
University of Texas Southwestern Medical Center, Department of Pediatrics, 5323 Harry Hines Blvd., Dallas, TX 75390-9063, USA. felix.shardonofsky@utsouthwestern.edu
Capetanaki
Yassemi
Y
Boriek
Aladin M
AM
D000403:Airway Resistance / Q000502:physiology*; D000818:Animals; D003893:Desmin / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D004548:Elasticity; D008168:Lung / Q000502:physiology*; D008170:Lung Compliance / Q000187:drug effects / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D011175:Positive-Pressure Respiration; D015656:Respiratory Mechanics / Q000187:drug effects / Q000502:physiology
D003893:Desmin