Synthesis and evaluation of an N-acylated photoactivatable analogue of glutathione as probe for glutathione-utilizing enzymes.
The first synthesis of an N-acylated photoactivatable analogue of reduced glutathione is described. N-(4-Benzoylbenzoyl)glutathione (8) was found to be an inhibitor and a photoaffinity probe of purified rat liver glutathione S-transferases.
Bernardi
Dan
D
Laboratoire d'Ingénierie Moléculaire et Biochimie Pharmacologique, Université Paul Verlaine-Metz, 1 boulevard Arago, 57078 METZ Cedex 3, France.
Battaglia
Eric
E
Kirsch
Gilbert
G
D000345:Affinity Labels; D000818:Animals; D005978:Glutathione / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000378:metabolism; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D051381:Rats
D000345:Affinity Labels; D005982:Glutathione Transferase; D005978:Glutathione