Sugar-induced tolerance to the herbicide atrazine in Arabidopsis seedlings involves activation of oxidative and xenobiotic stress responses.
Exogenous sucrose confers to Arabidopsis seedlings a very high level of tolerance to the herbicide atrazine that cannot be ascribed to photoheterotrophic growth. Important differences of atrazine tolerance between sucrose and glucose treatments showed that activation of chloroplast biogenesis per se could not account for induced tolerance. Sucrose-induced acquisition of defence mechanisms was shown by the gene expression pattern of a chloroplastic iron superoxide dismutase and by enhancement of whole-cell glucose-6-phosphate dehydrogenase activity. Activation of these defence mechanisms depended on both soluble sugar and atrazine. Moreover, acquisition of sucrose protection was shown to unmask atrazine-induced gene expression, such as that of a cytosolic glutathione-S-transferase, which remained otherwise cryptic because of the lethal effects of atrazine in the absence of soluble sugars.
Sulmon
Cécile
C
Centre National de la Recherche Scientifique, Université de Rennes 1, UMR 6553 ECOBIO, Campus de Beaulieu, bâtiment 14A, F-35042, Rennes Cedex, France.
Gouesbet
Gwenola
G
Amrani
Abdelhak El
AE
Couée
Ivan
I
D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D001280:Atrazine / Q000494:pharmacology*; D002736:Chloroplasts / Q000187:drug effects / Q000502:physiology; D004789:Enzyme Activation; D005947:Glucose / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology*; D018384:Oxidative Stress; D036226:Seedlings / Q000187:drug effects / Q000502:physiology; D013395:Sucrose / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D015262:Xenobiotics / Q000494:pharmacology*
D029681:Arabidopsis Proteins; D006540:Herbicides; D015262:Xenobiotics; D013395:Sucrose; D013482:Superoxide Dismutase; C483103:GSTF8 protein, Arabidopsis; D005982:Glutathione Transferase; D005947:Glucose; D001280:Atrazine