Two dimensional patterning of fluorescent proteins in hydrogels.
This work describes the successful micropatterning of hybrid systems consisting of hydrogel-dispersed optically active and controllable proteins on solid surfaces without degradation of the photophysical properties of the light-emitting biomolecules. It demonstrates the preservation of the luminescence properties of proteins entrapped into isolated microstructures of poly(acrylamide) gel. This way we can exploit both the structural and function-preserving properties of the hydrogels and the functionality of light-emitting proteins. We believe that this approach can open the way to the realization of nanopatterned optical memories based on photochromic biomolecules.
Di Benedetto
Francesca
F
National Nanotechnology Laboratory of Instituto Nazionale di Fisica della Materia-CNR, University of Lecce, I-73100 Lecce, Italy. Francesca.dibenedetto@unile.it
Biasco
Adriana
A
Bizzarri
Ranieri
R
Arosio
Daniele
D
Ricci
Fernanda
F
Beltram
Fabio
F
Cingolani
Roberto
R
Pisignano
Dario
D
D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D020100:Hydrogels; D066298:In Vitro Techniques; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D013499:Surface Properties
D005456:Fluorescent Dyes; D020100:Hydrogels; C485184:enhanced green fluorescent protein; C485192:green fluorescent protein, Aequorea victoria; D049452:Green Fluorescent Proteins