Identification of sugarcane genes induced in disease-resistant somaclones upon inoculation with Ustilago scitaminea or Bipolaris sacchari.
To understand the molecular basis of a specific plant-pathogen interaction, it is important to identify plant genes that respond to the pathogen attack. Amplified fragment length polymorphism (AFLP) analysis of cDNA was used to identify sugarcane genes differentially expressed in disease-resistant but not in susceptible sugarcane somaclones in response to inoculation with either Ustilago scitaminea or Bipolaris sacchari (also known as Helminthosporium sacchari or Drechslera sacchari), causal agents of smut and eyespot respectively. In total 62 differentially regulated genes were identified, of which 10 were down-regulated and 52 were induced. Of these 52, 19 transcript derived fragments showed homology to known plant gene sequences, most of them related to defense or signaling. The total set of differentially expressed sugarcane genes can be an important resource for further studies aimed at understanding sugarcane pathogen defense.
Borrás-Hidalgo
Orlando
O
Laboratory of Plant Functional Genomics, Center for Genetic Engineering and Biotechnology, P. O. Box 6162, Havana 10600, Cuba. orlando.borras@cigb.edu.cu
Thomma
Bart P H J
BP
Carmona
Elva
E
Borroto
Carlos J
CJ
Pujol
Merardo
M
Arencibia
Ariel
A
Lopez
Junior
J
D001203:Ascomycota / Q000502:physiology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D010935:Plant Diseases / Q000382:microbiology*; D031786:Saccharum / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D014588:Ustilago / Q000502:physiology*