Identification of a new class of recombinant prolamin genes in wheat.
A novel storage protein gene with obvious [corrected] chimeric structure was isolated from an immature kernel-specific cDNA library prepared from the old Hungarian wheat [corrected] variety, Bánkúti 1201. This clone contains gamma-gliadin sequences in the 5' region and LMW-glutenin sequences on the 3' end. A frameshift mutation was also introduced by the putative recombination event. Hence, the amino acid sequence of the C-terminal region was transformed to a completely new polypeptide. Based on this finding, 7 additional recombinant prolamin genes of similar structure were isolated with specific PCR primers. The 8 chimeric clones seem to be derived from 4 individual gamma-gliadin and 3 LMW-glutenin sequences. These genes show remarkable diversity in size, gliadin:glutenin ratio, frameshift mutations, and sulphur content. The putative functional characteristics of the chimeric polypeptides and problems related to the origin of the encoding genes are discussed.
Nagy
Ila J
IJ
Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, Hungary. taki@mail.mgki.hu
Takács
Imre
I
Juhász
Angéla
A
Tamás
László
L
Bedo
Zoltán
Z
D000595:Amino Acid Sequence; D001483:Base Sequence; D016368:Frameshift Mutation; D015723:Gene Library; D017343:Genes, Plant; D005903:Gliadin / Q000235:genetics; D005983:Glutens / Q000235:genetics; D008969:Molecular Sequence Data; D050506:Mutant Chimeric Proteins / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D055315:Prolamins; D011995:Recombination, Genetic; D014908:Triticum / Q000235:genetics*
D050506:Mutant Chimeric Proteins; D010940:Plant Proteins; D055315:Prolamins; D005983:Glutens; D005903:Gliadin; C037302:glutenin