High background radiation areas of Ramsar in Iran: evaluation of DNA damage by alkaline single cell gel electrophoresis (SCGE).
The hot springs in special areas in Ramsar, a northern coastal town in Iran, contain (226)Ra and (222)Rn. The natural radiation effects, radiosensitivity or adaptive responses, on the inhabitants of high natural radiation in Ramsar were studied. The single cell gel electrophoresis was used to monitor DNA damages. Three groups of volunteers were selected, one from high natural background radiation areas as the case group and two from normal background radiation areas as controls (control 1 and control 2). The latter one had the similar living situation to case group while the other (control 2) had different living situation from the other groups. Peripheral blood mononuclear cells (PMNCs) were separated and irradiated by (60)Co source at five different gamma doses. It was found that the spontaneous level of DNA damage and the induced DNA damage in all challenging doses in case group was considerably higher than control groups (p < 0.05). On the other hand, the repair rate in those volunteers, who received less than 10.2 mSv/y was significantly more than the control groups. In the contrary, individuals who live in homes with more than 10.2 mSv/y had incomplete repair. Additionally the plasma and urinary levels of vitamin C were measured spectrophotometrically. Although the concentration of vitamin C of plasma was equal in case and control 1 groups, the urinary level of vitamin C was found to be lower in the case group.
Masoomi
J R
JR
Biophysics Department, College of Sciences, Tarbiat Modarres University, Tehran, Iran.
Mohammadi
Sh
Sh
Amini
M
M
Ghiassi-Nejad
M
M
D001205:Ascorbic Acid / Q000097:blood / Q000652:urine; D020552:Comet Assay; D004249:DNA Damage; D006801:Humans; D007492:Iran; D011848:Radioactive Pollutants / Q000633:toxicity*
D011848:Radioactive Pollutants; D001205:Ascorbic Acid