Combined treatment of an intratumoral injection of dendritic cells and systemic chemotherapy (Paclitaxel) for murine fibrosarcoma.
A novel combined treatment of conventional chemotherapy with an intratumoral injection of syngeneic dendritic cells (DCs) has emerged as a potent cancer treatment strategy. In this study, we evaluated the synergistic effect of an intraperitoneal (i.p.) injection of a chemotherapeutic drug, paclitaxel, and an intratumoral (i.t.) injection of syngeneic bone marrow-derived DCs for the treatment of pre-existing fibrosarcoma. Subcutaneous tumors were established using MCA102 fibrosarcoma cells in syngeneic C57BL/6 mice. The results demonstrated that the combined treatment of paclitaxel chemotherapy and the injection of DCs led to complete tumor regression, in contrast to only partial eradication of the tumors with chemotherapy or DCs alone. Furthermore, the tumor-free mice were able to resist a repeat challenge with the same type of tumor. These findings suggest that a combination therapy of systemic chemotherapy along with the intratumoral administration of DCs is a potent treatment strategy for fibrosarcoma.
Choi
Gwang-Seong
GS
Clinical Research Center, Inha University College of Medicine, 7-206 3-ga, Jung-gu, Sinheung-dong, Incheon 400-711, Korea.
Lee
Moon-Hee
MH
Kim
Soon-Ki
SK
Kim
Chul-Soo
CS
Lee
Hong-Sik
HS
Im
Moon-Whan
MW
Kil
Hye-Yun
HY
Seong
Do-Hwan
DH
Lee
Jong-Rok
JR
Kim
Woo-Chul
WC
Lee
Min-Geol
MG
Song
Sun U
SU
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000627:therapeutic use*; D001854:Bone Marrow Cells / Q000166:cytology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003131:Combined Modality Therapy; D003713:Dendritic Cells / Q000166:cytology / Q000637:transplantation*; D005354:Fibrosarcoma / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D007156:Immunologic Memory; D007274:Injections, Intraperitoneal; D051379:Mice; D017239:Paclitaxel / Q000008:administration & dosage / Q000627:therapeutic use*; D010641:Phenotype; D014185:Transplantation, Isogeneic; D016896:Treatment Outcome
D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel