Experimental and theoretical aspects of glucose measurement using a microcantilever modified by enzyme-containing polyacrylamide.
We report a glucose oxidase-containing polyacrylamide hydrogel-coated microcantilever sensor for the measurement of glucose. This enzymatic reaction of glucose results in swelling of the hydrogel due to formation of charged ions (gluconate molecules and protons). The microcantilever undergoes reversible and reproducible bending deflection upon exposure to solutions containing various glucose concentrations due to swelling or shrinking of the hydrogels. The microcantilever deflections increase when the glucose concentrations increase. A theoretical model has been built to correlate volume changes of the gel with microcantilever bending. The calculated data matched with the experimental results very well. Such hydrogel-coated microcantilevers could potentially be used to prepare microcantilever-based chemical and biological sensors when other enzymes are immobilized in the hydrogel.
Ji
Hai-Feng
HF
Department of Chemistry, Louisiana Tech University, Ruston, Louisiana 71272, USA. hji@chem.latech.edu
Yan
Xiaodong
X
McShane
Michael J
MJ
D000180:Acrylic Resins; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D006801:Humans; D013499:Surface Properties
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; C016680:polyacrylamide gels; D005949:Glucose Oxidase; D005947:Glucose