Quorum-sensing signal synthesis by the Yersinia pestis acyl-homoserine lactone synthase YspI.
The acyl-homoserine lactone molecular species (AHLs) produced by the Yersinia pestis AHL synthase YspI were identified by biochemical and physical/chemical techniques. Bioassays of extracts from culture supernatants of the recombinant YspI and wild-type Yersinia pestis showed similar profiles of AHLs. Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-L-homoserine lactone and N-3-oxo-hexanoyl-L-homoserine lactone.
Kirwan
J Paul
JP
Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, P.O. Box 8511 MS8303, Aurora, CO 80045, USA.
Gould
Ty A
TA
Schweizer
Herbert P
HP
Bearden
Scott W
SW
Murphy
Robert C
RC
Churchill
Mair E A
ME
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D015010:Yersinia pestis / Q000201:enzymology / Q000502:physiology*
D011994:Recombinant Proteins; C088386:homoserine lactone; C030654:N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone; D008025:Ligases; C449816:N-acylhomoserine lactone synthase; D015107:4-Butyrolactone