title: Evolution of EV71 genogroup in Taiwan from 1998 to 2005: an emerging of subgenogroup C4 of EV71.
abstract: In Taiwan, enterovirus 71 (EV71) has played an important role in severe enterovirus-related cases every year since the devastating outbreak in 1998. Three genogroups A, B, C occur worldwide; with the B and C genogroups being subdivided into B1-B4 and C1-C4 subgenogroups respectively. To understand the mutation of the EV71 genogroup in Taiwan before and after 1998, a total of 54 worldwide strains were studied including 41 Taiwanese strains obtained in 1986 and 1998-2004. A fragment of 207 bp of the VP4 region was amplified and sequenced. Genetic analysis was performed using MEGA software (version 3.0) for the nucleotide sequence alignment and phylogenetic analysis. In Taiwan, the subgenogroup B1 was predominant before 1998 while subgenogroup C2 was the major etiologic group in 1998 outbreak. A minor etiologic group outbreak in 1998, subgenogroup B4, became predominant during the period from 1999 to 2003. In this study, subgenogroup C4 emerged and became predominant in 2004 in Taiwan. The nucleotide differences between B1 and C2, C2 and B4, B4 and C4 were 20%-26%, 19%-27%, 18%-22%, respectively. Nucleotide sequence alignment revealed 67 substitutions. Most of the substitutions (62/67) were silent mutations. This is the first report about the emergence of EV71 subgenogroup C4 in Taiwan.
authors.affiliation: Department of Clinical Laboratory, Kaohsiung Medical University, Kaohsiung City, Taiwan. kuhsli@ms3.hinet.net
authors.forename: Kuei-Hsiang
authors.initials: KH
authors.lastname: Lin
authors.forename: Kao-Pin
authors.initials: KP
authors.lastname: Hwang
authors.forename: Guan-Ming
authors.initials: GM
authors.lastname: Ke
authors.forename: Chu-Feng
authors.initials: CF
authors.lastname: Wang
authors.forename: Liang-Yin
authors.initials: LY
authors.lastname: Ke
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Hsu
authors.forename: Yi-Chin
authors.initials: YC
authors.lastname: Tung
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Chu
authors.forename: Bai-Hsiun
authors.initials: BH
authors.lastname: Chen
authors.forename: Hsiu-Lin
authors.initials: HL
authors.lastname: Chen
authors.forename: Chuan-Liang
authors.initials: CL
authors.lastname: Kao
authors.forename: Jen-Ren
authors.initials: JR
authors.lastname: Wang
authors.forename: Hock-Liew
authors.initials: HL
authors.lastname: Eng
authors.forename: Sheng-Yu
authors.initials: SY
authors.lastname: Wang
authors.forename: Li-Ching
authors.initials: LC
authors.lastname: Hsu
authors.forename: Hour-Young
authors.initials: HY
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D004196:Disease Outbreaks; D004770:Enterovirus / Q000235:genetics*; D004769:Enterovirus Infections / Q000453:epidemiology*; D019143:Evolution, Molecular; D005814:Genes, Viral; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013045:Species Specificity; D013624:Taiwan / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins