title: Excitation of the L intermediate of bacteriorhodopsin: electric responses to test x-ray structures.
abstract: The L intermediate of bacteriorhodopsin was excited, and its electrical response was measured. Two positive components were found in it with respect to the direction of proton pumping: an unresolved fast component, and a slower one (tau=7 micros) of small amplitude. The fast component was assigned to a charge motion corresponding to reisomerization of the retinal moiety, whereas the slow one was attributed to charge rearrangements reestablishing the ground state. Because three x-ray crystallographic structures have recently been reported for the L intermediate, it seemed important to calculate the intramolecular dipole moment changes associated to bR-->L for all three structures, so as to compare them with similar quantities determined from the electrical signals. The results are discussed in terms of amino acid side chains possibly contributing to the observed effect. We propose to use electrical signals as a verification tool for intermediate structures of the photocycle, and thus for molecular models of proton pumping.
authors.affiliation: Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: R
authors.initials: R
authors.lastname: Tóth-Boconádi
authors.forename: A
authors.initials: A
authors.lastname: Dér
authors.forename: S G
authors.initials: SG
authors.lastname: Taneva
authors.forename: L
authors.initials: L
authors.lastname: Keszthelyi
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry*; D001692:Biological Transport; D004396:Coloring Agents / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004594:Electrophysiology; D019617:Halobacterium salinarum / Q000378:metabolism*; D025602:Halorhodopsins / Q000737:chemistry; D007834:Lasers; D008027:Light; D008954:Models, Biological; D008958:Models, Molecular; D008968:Molecular Conformation; D017494:Proton Pumps; D011522:Protons; D013997:Time Factors; D014965:X-Rays
chemical_list: D004396:Coloring Agents; D025602:Halorhodopsins; D017494:Proton Pumps; D011522:Protons; D001436:Bacteriorhodopsins