title: Microbial incorporation of 13C-labeled acetate at the field scale: detection of microbes responsible for reduction of U(VI).
abstract: A field-scale acetate amendment experiment was performed in a contaminated aquifer at Old Rifle, CO to stimulate in situ microbial reduction of U(VI) in groundwater. To evaluate the microorganisms responsible for microbial uranium reduction during the experiment, 13C-labeled acetate was introduced into well bores via bio-traps containing porous activated carbon beads (Bio-Sep). Incorporation of the 13C from labeled acetate into cellular DNA and phospholipid fatty acid (PLFA) biomarkers was analyzed in parallel with geochemical parameters. An enrichment of active sigma-proteobacteria was demonstrated in downgradient monitoring wells: Geobacter dominated in wells closer to the acetate injection gallery, while various sulfate reducers were prominent in different downgradient wells. These results were consistent with the geochemical evidence of Fe(III), U(VI), and SO(4)2- reduction. PLFA profiling of bio-traps suspended in the monitoring wells also showed the incorporation of 13C into bacterial cellular lipids. Community composition of downgradient monitoring wells based on quinone and PLFA profiling was in general agreement with the 13C-DNA result. The direct application of 13C label to biosystems, coupled with DNA and PLFA analysis,
authors.affiliation: University of Tennessee, Knoxville, USA. ychang1@utk.edu
authors.forename: Yun-Juan
authors.initials: YJ
authors.lastname: Chang
authors.forename: Philip E
authors.initials: PE
authors.lastname: Long
authors.forename: Roland
authors.initials: R
authors.lastname: Geyer
authors.forename: Aaron D
authors.initials: AD
authors.lastname: Peacock
authors.forename: Charles T
authors.initials: CT
authors.lastname: Resch
authors.forename: Kerry
authors.initials: K
authors.lastname: Sublette
authors.forename: Susan
authors.initials: S
authors.lastname: Pfiffner
authors.forename: Amanda
authors.initials: A
authors.lastname: Smithgall
authors.forename: Robert T
authors.initials: RT
authors.lastname: Anderson
authors.forename: Helen A
authors.initials: HA
authors.lastname: Vrionis
authors.forename: John R
authors.initials: JR
authors.lastname: Stephen
authors.forename: Richard
authors.initials: R
authors.lastname: Dayvault
authors.forename: Irene
authors.initials: I
authors.lastname: Ortiz-Bernad
authors.forename: Derek R
authors.initials: DR
authors.lastname: Lovley
authors.forename: David C
authors.initials: DC
authors.lastname: White
mesh_terms: D000085:Acetates / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002247:Carbon Isotopes / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D045302:Geobacter / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020560:Proteobacteria / Q000378:metabolism*; D014501:Uranium / Q000378:metabolism*
chemical_list: D000085:Acetates; D002247:Carbon Isotopes; D014501:Uranium