Tissue-specific Spred-2 promoter activity characterized by a gene trap approach.
Spreds (Sprouty-related proteins with an Ena/Vasodilator-stimulated phosphoprotein homology-1 domain) are a new protein family inhibiting the mitogen-activated protein kinase (MAPK) signaling pathway. Different RNA and protein studies already revealed an almost ubiquitous Spred-2 expression pattern. But until now, only few data were available on the in situ Spred-2 promoter activity. Here, we show a detailed in situ analysis of a mouse strain with a trapped Spred-2 gene, bringing a beta-galactosidase and neomycin fusion gene (beta-geo) under the control of the endogenous Spred-2 promoter. This allowed us to monitor Spred-2 promoter activity in practically every organ and their corresponding sub-compartments. X-Gal staining of newborn and adult mice revealed a nearly congruent Spred-2 promoter activity pattern. Our detailed data provide information for further studies of the still enigmatic physiological functions of Spred-2 in various organs by identifying the tissues with strong Spred-2 promoter activity.
Bundschu
Karin
K
Institut für Klinische Biochemie und Pathobiochemie, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany.
Gattenlöhner
Stefan
S
Knobeloch
Klaus-Peter
KP
Walter
Ulrich
U
Schuh
Kai
K
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005260:Female; D005697:Galactosides / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D017930:Genes, Reporter; D005822:Genetic Vectors; D007150:Immunohistochemistry; D007211:Indoles / Q000378:metabolism; D007438:Introns; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D009130:Muscle, Smooth / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology
D018076:DNA, Complementary; D005697:Galactosides; D007211:Indoles; D012097:Repressor Proteins; C433764:Spred2 protein, mouse; D020928:Mitogen-Activated Protein Kinases; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside