Rapid identification of Ochroconis gallopava by a loop-mediated isothermal amplification (LAMP) method.
Ochroconis gallopava is a species of dematiaceous fungi recognized as a causative agent of zoonotic and emerging fungal infections. It affects the central nervous system and respiratory tracts of humans, birds and cats. We designed O. gallopava species-specific primer sets to aid in its identification by a loop-mediated isothermal amplification (LAMP) method based on the D1/D2 domain of the LSU rDNA sequence. The LAMP method successfully detected the gene from both fungal DNA and experimentally infected brains and spleens of mice and will be helpful in the diagnosis of O. gallopava infection.
Ohori
Akira
A
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chiba 260-8673, Japan.
Endo
Shigeo
S
Sano
Ayako
A
Yokoyama
Koji
K
Yarita
Kyoko
K
Yamaguchi
Masashi
M
Kamei
Katsuhiko
K
Miyaji
Makoto
M
Nishimura
Kazuko
K
D000818:Animals; D003883:Arthrodermataceae / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D004271:DNA, Fungal / Q000032:analysis*; D003881:Dermatomycoses / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D051379:Mice; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013045:Species Specificity
D004271:DNA, Fungal