title: Identification of nitrated proteins in Alzheimer's disease brain using a redox proteomics approach.
abstract: Nitric oxide (NO) has been implicated in the pathophysiology of a number of neurodegenerative diseases including Alzheimer's disease (AD). In the present study, using a proteomics approach, we identified enolase, glyceraldehyde-3-phosphate dehydrogenase, ATP synthase alpha chain, carbonic anhydrase-II, and voltage-dependent anion channel-protein as the targets of nitration in AD hippocampus, a region that shows a extensive deposition of amyloid beta-peptide, compared with the age-matched control brains. Immunoprecipitation and Western blotting techniques were used to validate the correct identification of these proteins. Our results are discussed in context of the role of oxidative stress as one of the important mechanisms of neurodegeneration in AD.
authors.affiliation: Department of Chemistry, University of Kentucky, Lexington, KY 40506, USA.
authors.forename: Rukhsana
authors.initials: R
authors.lastname: Sultana
authors.forename: H Fai
authors.initials: HF
authors.lastname: Poon
authors.forename: Jian
authors.initials: J
authors.lastname: Cai
authors.forename: William M
authors.initials: WM
authors.lastname: Pierce
authors.forename: Michael
authors.initials: M
authors.lastname: Merchant
authors.forename: Jon B
authors.initials: JB
authors.lastname: Klein
authors.forename: William R
authors.initials: WR
authors.lastname: Markesbery
authors.forename: D Allan
authors.initials: DA
authors.lastname: Butterfield
mesh_terms: D025181:ATP Synthetase Complexes / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D024402:Carbonic Anhydrase II / Q000378:metabolism; D005260:Female; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D008297:Male; D009410:Nerve Degeneration / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods*; D014443:Tyrosine / Q000378:metabolism; D050995:Voltage-Dependent Anion Channel 1 / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins; D009566:Nitrates; C494762:VDAC1 protein, human; D014443:Tyrosine; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); D050995:Voltage-Dependent Anion Channel 1; D025181:ATP Synthetase Complexes; D024402:Carbonic Anhydrase II; D010751:Phosphopyruvate Hydratase