Monoamine oxidase inhibitory constituents from the fruits of Cudrania tricuspidata.
A methylene chloride soluble fraction of the fruits of Cudrania tricuspidata significantly inhibited the mouse brain monoamine oxidase (MAO). Three known prenylated isoflavones were isolated and identified by activity-guided fractionation. Gancaonin A (1), 4'-O-methylalpinumisoflavone (2), and alpinumisoflavone (3) inhibited MAO activity in a concentration-dependent manner with IC50 values of 19.4, 23.9, and 25.8 microM, respectively. Of these, gancaonin A (1) showed a selective and potent inhibitory effect against MAO-B (IC50 0.8 microM) than MAO-A (IC50 >800 microM). The kinetic analysis using Lineweaver-Burk plots indicated that gancaonin A (1) competitively inhibited MAO-B.
Han
Xiang Hua
XH
College of Pharmacy, Chungbuk National University, Cheongju Korea.
Hong
Seong Su
SS
Hwang
Ji Sang
JS
Jeong
Seon Hwa
SH
Hwang
Ji Hye
JH
Lee
Min Hee
MH
Lee
Myung Koo
MK
Lee
Dongho
D
Ro
Jai Seup
JS
Hwang
Bang Yeon
BY
D000818:Animals; D001921:Brain / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007700:Kinetics; D031624:Maclura; D008752:Methylene Chloride / Q000737:chemistry; D051379:Mice; D008995:Monoamine Oxidase / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry
C507553:4'-O-methylalpinumisoflavone; D007529:Isoflavones; D008996:Monoamine Oxidase Inhibitors; D010936:Plant Extracts; C507552:gancaonin A; D008752:Methylene Chloride; C000154:alpinumisoflavone; D008995:Monoamine Oxidase