[In vitro inhibitory effect of 23-HBA on angiogenesis].
OBJECTIVE
To study the inhibitory effect of 23-HBA on angiogenesis in vitro.


METHODS
The effect of 23-hydroxy butulinic acid (23-HBA) on the in vitro proliferation of human microcapillary endothelial cells(HMECs) was examined by sulfonylrhodamine B (SRB) assay. The effect of 23-HBA on endothelial cell migration, and tubule formation on Matrigel was also observed. The CD31 expression in HMECs was dectected by immunohistochemical staining.


RESULTS
The proliferation of HMECs was inhibited significantly by 23-HBA with IC(50) being 40.44 mg/L. 23-HBA inhibited endothelial cell migration and tubule formation in a dose-dependent manner. The expression of CD31 in HMECs was reduced after treatment with 10 mg/L 23-HBA.


CONCLUSIONS
23-HBA can inhibit angiogenesis in vitro, which would become a promising antiangiogenic drug.
Jiang
Meng-jun
MJ
State Key Laboratory of Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China. jmj16888@yahoo.com.cn
Yang
Min
M
Zhou
Yao-yuan
YY
Zhang
Rong-jun
RJ
Cao
Guo-xian
GX
Cai
Gang-ming
GM
Wang
Guang-ji
GJ
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology*
C468651:23-hydroxybetulinic acid; D020533:Angiogenesis Inhibitors; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014315:Triterpenes