Adaptive response induced by lipid peroxidation products in cell cultures.
The adaptive response induced by the lipid peroxidation products, such as phosphatidylcholine hydroperoxide, lysophosphatidylcholine (LysoPC), 15-deoxy-Delta(12,14)-prostaglandin J(2), 4-hydroxynonenal (4-HNE), hydroxyoctadecadienoic acid, 7-hydroxycholesterol, and cholesterol 5beta,6beta-epoxide, was investigated in this study. Although these products have been implicated in oxidative stress-related diseases, pretreatment with such compounds at sublethal concentrations significantly protected PC12 cells against subsequent oxidative stress induced by 6-hydroxydopamine. Moreover, 4-HNE and LysoPC also exhibited adaptive protection in human arterial endothelial cells. These findings suggest a general hormetic effect of such compounds in cell cultures and may lead to a reappraisal of the eventual role of reactive oxygen species and lipid peroxidation in organisms.
Chen
Zhi-Hua
ZH
Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology, Midorigaoka, Ikeda, Osaka 563-8577, Japan. zhihua-chen@aist.go.jp
Yoshida
Yasukazu
Y
Saito
Yoshiro
Y
Noguchi
Noriko
N
Niki
Etsuo
E
D018663:Adrenergic Agents / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D001158:Arteries / Q000033:anatomy & histology; D018929:Cell Culture Techniques; D015853:Cysteine Proteinase Inhibitors / Q000378:metabolism; D019610:Cytoprotection; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D015227:Lipid Peroxidation; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D018384:Oxidative Stress; D016627:Oxidopamine / Q000378:metabolism; D016716:PC12 Cells; D020011:Protective Agents / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D018663:Adrenergic Agents; D000447:Aldehydes; D015853:Cysteine Proteinase Inhibitors; D008244:Lysophosphatidylcholines; D020011:Protective Agents; D017382:Reactive Oxygen Species; D016627:Oxidopamine; C027576:4-hydroxy-2-nonenal