title: Evaluation of the assurance GDS for E. coli O157:H7 method and assurance GDS for shigatoxin genes method in selected foods: collaborative study.
abstract: A multilaboratory collaborative study was conducted to compare the Assurance GDS for E. coli 0157:H7 method and the reference culture methods for the detection of E. coli 0157:H7 in orange juice, raw ground beef, and fresh lettuce. A separate companion assay, the Assurance GDS for Shigatoxin Genes method was also evaluated with the same test portions. Fifteen laboratories participated in the study. A Chi square analysis of each of the 3 food types at the high, low, and uninoculated control levels was performed. For all foods, the Assurance GDS for E. coli O157:H7 method and the Assurance GDS for Shigatoxin Genes method were equivalent to or better than the reference methods.
authors.affiliation: BioControl Systems, Inc., 12822 SE 32nd St, Bellevue, WA 98005, USA. ptf@biocontrolsys.com
authors.forename: Philip T
authors.initials: PT
authors.lastname: Feldsine
authors.forename: Shannon T
authors.initials: ST
authors.lastname: Green
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Lienau
authors.forename: James
authors.initials: J
authors.lastname: Stephens
authors.forename: Markus T
authors.initials: MT
authors.lastname: Jucker
authors.forename: David E
authors.initials: DE
authors.lastname: Kerr
mesh_terms: D000818:Animals; D001628:Beverages / Q000032:analysis; D002957:Citrus; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005504:Food Analysis / Q000379:methods*; D005516:Food Microbiology; D018545:Lactuca; D008460:Meat / Q000032:analysis; D022621:Shiga Toxin / Q000032:analysis* / Q000235:genetics
chemical_list: D022621:Shiga Toxin