Fructose-1,6 diphosphate as a protective agent for experimental ischemic acute renal failure.
Cold ischemia time is a risk factor for the development of acute renal failure in the immediate post-transplant period. In this study, we aimed to determine if intravenous fructose-1,6-diphosphate (FDP), given before nephrectomy, attenuates renal cell injury in a cold ischemia model. Male adult Wistar rats were subjected to infusion of either FDP 350 mg/kg (group F, n=6), an equal volume of 0.9% NaCl (group S, n=6), an equal volume/osmolality of mannitol (group M, n=6) or no infusion (group C, n=7). Kidneys were then perfused in situ with Collins solution and nephrectomy was performed. Other kidney slices were stored in Collins solution at 4 degrees C. Adenosine triphosphate (ATP) levels and lactate dehydrogenase (LDH) release were examined at 0, 24, 48 and 72 h. Other slices, obtained after 50 min immersion in Collins solution at 37 degrees C, were frozen for characterization of cytoskeletal preservation using phalloidin-FITC staining. Apical fluorescence intensity of proximal tubule cells, indicative of the F-actin concentration, was measured in a fluorescence microscope interfaced with computer image analysis system. Adenosine triphosphate levels, after up to 72 h of tissue incubation, were higher (P<0.05) in the FDP group when compared to other groups. In addition, LDH release was smaller (P<0.0001) in the FDP group. The F-actin concentration of proximal tubule cells cells was greater in the FDP group (P<0.0001). Results indicate that FDP is a useful tool to increase tissue viability in a rat kidney subjected to cold ischemia, by maintaining ATP cell content, decreasing LDH release and preventing microfilament disruption of proximal tubule cells.
Antunes
N
N
Universidade Federal do Rio de Janeiro: Nefrologica, HUCFF, Rio de Janeiro, Brazil.
Martinusso
C A
CA
Takiya
C M
CM
da Silva
A J R
AJ
de Ornellas
J F R
JF
Elias
P R
PR
Leite
M
M
Cardoso
L R
LR
D058186:Acute Kidney Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D005635:Fructosediphosphates / Q000627:therapeutic use*; D007511:Ischemia / Q000150:complications*; D007668:Kidney / Q000098:blood supply*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005635:Fructosediphosphates; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; C029063:fructose-1,6-diphosphate