title: Using urea dilution to standardise cellular and non-cellular components of pleural and bronchoalveolar lavage (BAL) fluids in the cat.
abstract: A technique to standardise the analysis of cellular and non-cellular components in epithelial lining fluid (ELF) collected during saline lavage of pulmonary and pleural cavities was developed using the urea dilution method. Bronchoalveolar lavage (BAL) and pleural lavage (PL) fluids were collected from 12 clinically healthy cats. Total and differential cell counts in BAL fluid were within normal ranges for the cat, while cell counts in PL fluid were assumed to be normal based on clinical health during examination, auscultation and lactate dehydrogenase (LDH) activities being comparable with other species. The major clinical implication of this study was that nucleated cell counts within feline ELF could not be predicted from analysis of lavage fluid which suggests that calculation of the proportion of ELF in lavage fluid by the urea dilution method may be necessary to avoid misdiagnosis of health or disease in pulmonary or pleural cavities.
authors.affiliation: School of Veterinary Science, University of Queensland, Brisbane, Australia. p.mills@uq.edu.au
authors.forename: Paul C
authors.initials: PC
authors.lastname: Mills
authors.forename: Annette
authors.initials: A
authors.lastname: Litster
mesh_terms: D000818:Animals; D001772:Blood Cell Count / Q000379:methods / Q000662:veterinary*; D001826:Body Fluids / Q000166:cytology*; D018893:Bronchoalveolar Lavage / Q000592:standards / Q000662:veterinary*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology*; D002415:Cats; D003951:Diagnostic Errors / Q000517:prevention & control / Q000662:veterinary; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D008297:Male; D010994:Pleura / Q000737:chemistry; D011650:Pulmonary Alveoli / Q000166:cytology*; D012016:Reference Values; D012140:Respiratory Tract Diseases / Q000175:diagnosis / Q000662:veterinary; D007507:Therapeutic Irrigation / Q000662:veterinary; D014508:Urea / Q000032:analysis* / Q000737:chemistry
chemical_list: D014508:Urea