Application of magnetic resonance diffusion anisotropy imaging for the assessment neuroprotecting effects of MPEP, a selective mGluR5 antagonist, on the rat spinal cord injury in vivo.
Magnetic resonance diffusion anisotropy imaging (DAI) of the rat spinal cord after contusion using weight-drop method was used to study the neuroprotecting effect of 2-methyl-6-(phenylethynyl)-pyridine (MPEP), an mGluR5 receptor antagonist. Eighteen rats were used, divided into 3 groups of 6 animals: a reference group without any operation, a control group with injury and a test group with injury and MPEP. DAI was performed at 4.7 T at 1 h, 24 h, 48 h and 7 day after the injury. Locomotor function was evaluated using Basso, Beattie and Bresnahan (BBB) open field locomotor activity test each day starting one day after the injury. DAI results confirm positive effect of MPEP on the limitation of secondary excitotoxic injury in the spinal cord.
Banasik
Tomasz
T
H. Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, PL 31-342 Kraków, Poland.
Jasiński
Andrzej
A
Pilc
Andrzej
A
Majcher
Katarzyna
K
Brzegowy
Paweł
P
D000818:Animals; D016880:Anisotropy; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods; D004195:Disease Models, Animal; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D011725:Pyridines / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
C578937:Grm5 protein, rat; D018696:Neuroprotective Agents; D011725:Pyridines; D064529:Receptor, Metabotropic Glutamate 5; D018094:Receptors, Metabotropic Glutamate; C121465:6-methyl-2-(phenylethynyl)pyridine