title: [The effect on the differentiation and maturation of dendritic cells by lupane acetate of cortex periplocae].
abstract: OBJECTIVE
  To investigate the effect of lupane acetate of cortex periplocae (CPLA) on the differentiation, maturation and immune activity of human peripheral blood mononuclear cell (PBMC)-derived dendritic cells (DCs) in vitro.
  
  
  METHODS
  PBMC isolated from human peripheral blood was cultured with GM-CSF, IL-4 for 5 d and stimulated with TNF-alpha (as positive control) or CPLA to induce DCs. The morphological characteristics of DC were observed under inverted microscope and transmisson electron microscope. The expressions of CD1a, CD83, CD80 and CD86 were analyzed by flow cytometry. IL-12, IFN-gamma production in the culture supernatant of DCs was detected by ELISA. MTT method was used to determine the proliferation of T cells stimulated by DCs.
  
  
  RESULTS
  After 10-days culture with cytokines and CPLA, PBMC developed into mature DCs with typical morphological characteristics and high expressions of CD1a, CD83, CD80 and CD86 on the cellular surface (P<0.05). CPLA enhanced IL-12 and IFN-gamma production by DCs (P<0.05). CPLA-treated DCs markedly stimulated proliferation of T cells (P<0.05).
  
  
  CONCLUSIONS
  CPLA may induce the differentiation and maturation of DC, up-regulate cytokines production and increase the immune activity of DC.
authors.affiliation: Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Bao-en
authors.initials: BE
authors.lastname: Shan
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Rui-nian
authors.initials: RN
authors.lastname: Zhao
authors.forename: Qi-liang
authors.initials: QL
authors.lastname: Li
authors.forename: Jiang-hui
authors.initials: JH
authors.lastname: Liu
authors.forename: Qiao-min
authors.initials: QM
authors.lastname: Li
authors.forename: Run-qing
authors.initials: RQ
authors.lastname: Li
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D018949:Antigens, CD1 / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D019684:Magnoliopsida / Q000737:chemistry*; D008562:Membrane Glycoproteins / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D010946:Plants, Medicinal / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*; D000097802:CD83 Antigen; D053978:Pentacyclic Triterpenes
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D004365:Drugs, Chinese Herbal; D007136:Immunoglobulins; D018664:Interleukin-12; D008562:Membrane Glycoproteins; D014315:Triterpenes; D000097802:CD83 Antigen; C419393:CD1a antigen; C480546:lupane; D053978:Pentacyclic Triterpenes