title: A comparative study of nitric oxide, glutathione, and glutathione peroxidase activities in cerebrospinal fluid from children with convulsive diseases/children with aseptic meningitis.
abstract: It has been reported that active oxygen and/or free radicals are produced in the central nervous system (CNS) compartment in patients with bacterial meningitis, so it is supposed that the levels of endogenous antioxidative scavengers in the cerebrospinal fluid (CSF) are elevated as an adaptive reaction to bacterial meningitis, which exerts severe stress on the human body. We assumed that they are also elevated in patients with convulsive diseases. Nitric oxide (NO) and endogenous antioxidative scavengers (glutathione (GSH), glutathione peroxidase (GPX), (total) superoxide dismutase (T-SOD), manganese superoxide dismutase (Mn-SOD), and catalase) were measured in CSF from a group of child patients with various neurological diseases and a control group. NO, GSH, and GPX activities in CSF from the patients with convulsive diseases were significantly higher than in those with aseptic meningitis or in the controls. Furthermore, all parameters in CSF from patients with bacterial meningitis were significantly higher than in any other group. The present study suggests that oxidative stress may be associated with the pathophysiology of convulsion and that its clinical attenuation will lead to improvement in the prognosis for convulsive diseases.
authors.affiliation: Department of Pediatrics, Ageo Central General Hospital, 1-10-10, Kashiwaza, Ageo, Saitama 362-8588, Japan. kawakami@nms.ac.jp
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Manami
authors.initials: M
authors.lastname: Monobe
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kuwabara
authors.forename: Takehisa
authors.initials: T
authors.lastname: Fujita
authors.forename: Miho
authors.initials: M
authors.lastname: Maeda
authors.forename: Osamu
authors.initials: O
authors.lastname: Fujino
authors.forename: Shuji
authors.initials: S
authors.lastname: Kojima
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Fukunaga
mesh_terms: D001921:Brain / Q000378:metabolism* / Q000503:physiopathology; D002374:Catalase / Q000134:cerebrospinal fluid; D002675:Child, Preschool; D004827:Epilepsy / Q000134:cerebrospinal fluid* / Q000503:physiopathology; D005260:Female; D016166:Free Radical Scavengers / Q000134:cerebrospinal fluid*; D005978:Glutathione / Q000134:cerebrospinal fluid; D005979:Glutathione Peroxidase / Q000134:cerebrospinal fluid; D006801:Humans; D007223:Infant; D008297:Male; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid* / Q000503:physiopathology; D009569:Nitric Oxide / Q000134:cerebrospinal fluid; D018384:Oxidative Stress / Q000502:physiology*; D003294:Seizures, Febrile / Q000134:cerebrospinal fluid* / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000134:cerebrospinal fluid
chemical_list: D016166:Free Radical Scavengers; D009569:Nitric Oxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione