title: In vivo imaging of tumor response to therapy using a dual-modality imaging strategy.
abstract: In vivo assessment of the outcome of cancer therapy is hampered by the paucity of imaging probes that target tumors specifically and noninvasively. The importance of such probes increases with the continuous development of chemotherapeutics and the necessity to evaluate their effectiveness in a clinical setting. We have recently reported on a dual-modality imaging probe specifically targeting the underglycosylated mucin-1 tumor-specific antigen (uMUC-1), which is one of the early hallmarks of tumorigenesis in a wide variety of tumors. This probe consists of crosslinked superparamagnetic iron oxide nanoparticles (CLIO) for MR imaging, modified with Cy5.5 dye (for near infrared optical fluorescence imaging (NIRF)), and has peptides (EPPT), specifically recognizing uMUC-1, attached to the nanoparticle's dextran coat. In the present study, we demonstrated that this probe could not only detect orthotopically implanted preclinical models of adenocarcinomas but could also track tumor response to chemotherapy in vivo in real time. Considering the high cost associated with the development and testing of new cancer therapeutics and the need for accurate, noninvasive assessment of their effectiveness, we believe that the developed probe represents a valuable research tool relevant to clinical discovery.
authors.affiliation: Molecular Imaging Laboratory, MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA.
authors.forename: Zdravka
authors.initials: Z
authors.lastname: Medarova
authors.forename: Wellington
authors.initials: W
authors.lastname: Pham
authors.forename: Young
authors.initials: Y
authors.lastname: Kim
authors.forename: Guangping
authors.initials: G
authors.lastname: Dai
authors.forename: Anna
authors.initials: A
authors.lastname: Moore
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000188:drug therapy; D000818:Animals; D000951:Antigens, Neoplasm; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D015415:Biomarkers / Q000032:analysis; D005260:Female; D005453:Fluorescence; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D007501:Iron; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D016513:Mice, SCID; D018396:Mucin-1; D009077:Mucins; D055095:Optics and Photonics; D010087:Oxides; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000188:drug therapy; D010446:Peptide Fragments; D016896:Treatment Outcome
chemical_list: D000951:Antigens, Neoplasm; D000964:Antimetabolites, Antineoplastic; D015415:Biomarkers; C060192:MUC1 protein, human; D018396:Mucin-1; D009077:Mucins; D010087:Oxides; D010446:Peptide Fragments; C094094:carboxydextran-coated magnetic iron oxide particles; D007501:Iron; D005472:Fluorouracil