title: A preliminary proteomic survey of the in vitro excretory/secretory products of fourth-stage larval and adult Teladorsagia circumcincta.
abstract: The nature of the proteins which comprise the in vitro excretory/secretory products (ES) of the fourth-stage larva (L4) and adult Teladorsagia circumcincta are largely undefined, despite the fact that this nematode induces profound changes, in part related to parasite ES, in the cellular architecture of the glands lining the abomasal surface of infected sheep and goats. In this study, the protein components of L4 and adult ES were fractionated using 1D gel electrophoresis and the major protein bands, detected by Coomassie blue staining, excised from the gel and subjected to tryptic digest and subsequent mass spectrometric analysis. The resultant peptide mass fingerprints were used to identify 15 L4 and 13 adult ES proteins. Several proteins, such as globin and some metabolic enzymes, were present in both ES. L4 ES alone contained thioredoxin peroxidase, an enzyme that can detoxify free radicals resulting from host inflammatory responses to the parasite, a cysteine proteinase which may aid penetration of the gastric mucosa and 2 different galectins which may influence cell differentiation and morphogenesis. Adult ES contained a nucleoside diphosphate kinase homologue, an enzyme which has been linked to cellular changes and can affect liquid secretion and goblet cell degranulation.
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, Scotland EH 26 0PZ, UK.
authors.forename: H
authors.initials: H
authors.lastname: Craig
authors.forename: J M
authors.initials: JM
authors.lastname: Wastling
authors.forename: D P
authors.initials: DP
authors.lastname: Knox
mesh_terms: D000818:Animals; D046508:Culture Techniques; D015801:Helminth Proteins / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism; D007814:Larva / Q000737:chemistry / Q000502:physiology; D013058:Mass Spectrometry / Q000662:veterinary; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000662:veterinary; D012756:Sheep; D014251:Trichostrongyloidea / Q000737:chemistry / Q000254:growth & development / Q000502:physiology*
chemical_list: D015801:Helminth Proteins