title: Characterization, occurrence, and molecular cloning of a lectin from Grifola frondosa: jacalin-related lectin of fungal origin.
abstract: A lectin named GFL was isolated from the fruiting body of the basidiomycete mushroom Grifola frondosa, which belongs to Aphyllophorales. The lectin had a molecular mass of 24 kDa on SDS-PAGE. The hemagglutinating activity of GFL was not inhibited by any monosaccharide, and inhibited only by porcine stomach mucin so far as tested. The occurrence of GFL was studied at three stages during fruiting body formation. The largest quantity of hemagglutinating activity was found in the fruiting body, and lesser amounts in the mycelial mat and the primordium. The 24-kDa band of GFL was found at all three stages, and the band-intensity corresponded to the level of activity in each sample. By cloning and sequencing the GFL-cDNA, the primary structure of this lectin was determined. GFL is composed of 181 amino acids, having no signal peptide. The amino acid sequence was found to be homologous to those of so-called jacalin-related plant lectins, suggesting that GFL is the first example of a jacalin-related lectin of fungal origin.
authors.affiliation: Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University, Matsudo, Japan. ynagata@faculty.chiba-u.jp
authors.forename: Yoshiho
authors.initials: Y
authors.lastname: Nagata
authors.forename: Masako
authors.initials: M
authors.lastname: Yamashita
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Honda
authors.forename: Junko
authors.initials: J
authors.lastname: Akabane
authors.forename: Koichi
authors.initials: K
authors.lastname: Uehara
authors.forename: Akihiro
authors.initials: A
authors.lastname: Saito
authors.forename: Futoshi
authors.initials: F
authors.lastname: Sumisa
authors.forename: Kozo
authors.initials: K
authors.lastname: Nishibori
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Oodaira
mesh_terms: D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000737:chemistry / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D030541:Databases, Genetic; D004591:Electrophoresis, Polyacrylamide Gel; D048691:Grifola / Q000737:chemistry*; D006386:Hemagglutination Tests; D037102:Lectins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D037121:Plant Lectins / Q000378:metabolism*; D017385:Sequence Homology
chemical_list: D018076:DNA, Complementary; D004271:DNA, Fungal; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin