Transposition of Regulatory Elements by P-Element-Mediated Rearrangements in Drosophila melanogaster.
Previously we described highly unstable mutations in the yellow locus, induced by the chimeric element and consisting of sequences from a distally located 1A unique genomic region, flanked by identical copies of an internally deleted 1.2-kb P element. Here we show that a sequence, which is part of the yellow 1A region, can be transmitted to the AS-C by successive inversion and reinversion generated by yellow- and AS-C-located P elements. The chimeric element contains a regulatory element from the 1A region that specifically blocks yellow wing and body enhancers and simultaneously stimulates yellow expression in bristles. These results suggest that P-element-generated chimeric elements may play a certain role in rapid changes of regulatory regions of genes during evolution.
Pomerantseva
Ekaterina
E
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Biryukova
Inna
I
Silicheva
Rita
R
Savitskaya
Ekaterina
E
Golovnin
Anton
A
Georgiev
Pavel
P
D000483:Alleles; D000818:Animals; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D019143:Evolution, Molecular; D018095:Germ-Line Mutation; D019476:Insect Proteins; D008957:Models, Genetic; D016254:Mutagenesis, Insertional; D010641:Phenotype; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D019476:Insect Proteins; D011993:Recombinant Fusion Proteins; D004247:DNA