title: HPLC retention behaviors of poly-aromatic-hydrocarbones on Cu(II)-octabromotetrakis(4-carboxyphenyl)porphine derivatives-immobilized aminopropyl silica gels in polar and non-polar eluents.
abstract: As one of approaches of developing novel HPLC stationary phases, we prepared Cu-octabromotetrakis(4-carboxyphenyl)porphine derivative-immobilized silica gels (Cu-OBTCPP(D)), and evaluated the availability of the resultant Cu-OBTCPP(D) as a stationary phase for separation of poly-aromatic-hydrocarbons (PAHs) and their related compounds. A Cu-OBTCPP(D) column was revealed to have an ability to separate simple PAHs and be useful as a stationary phase in both polar and non-polar eluents. The retention property of the Cu-OBTCPP(D) column was evaluated in various comparative experiments using commercially available columns. In comparison with 2-(1-pyrenyl)ethyl dimetylsilyl silica gel column (PYE column) regarding the retention behavior for PAHs etc., the Cu-OBTCPP(D) column showed stronger interactions involving pi electron in non-polar eluent than PYE column. In comparison with a pentabromobenzyloxy propylsilyl silica gel column (PBB column) regarding the influence of bromination, the Cu-OBTCPP(D) column was affected differently from the PBB column. In comparison with nitrophenylethyl silica gel column (NPE column) regarding the retention behavior for compounds having a dipole in a non-polar eluent, the Cu-OBTCPP(D) column showed electrostatic interactions such as dipole-dipole interaction equivalent to or larger than the NPE column.
authors.affiliation: Department of Pharmaceutical Sciences, Graduate School of Medicine and Dentistry and Pharmaceutical Sciences, Okayama University, Tsushima-Naka, Japan. mifune@pharm.okayama-u.ac.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Mifune
authors.forename: Kenji
authors.initials: K
authors.lastname: Kawata
authors.forename: Kurumi
authors.initials: K
authors.lastname: Tanaka
authors.forename: Youji
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Ikuko
authors.initials: I
authors.lastname: Tsukamoto
authors.forename: Madoka
authors.initials: M
authors.lastname: Saito
authors.forename: Jun
authors.initials: J
authors.lastname: Haginaka
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Saito
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004563:Electrochemistry; D007202:Indicators and Reagents; D008665:Metalloporphyrins / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry; D058428:Silica Gel; D012822:Silicon Dioxide
chemical_list: C508583:Cu(II)-octabromotetrakis(4-carboxyphenyl)porphine; D007202:Indicators and Reagents; D008665:Metalloporphyrins; D011084:Polycyclic Aromatic Hydrocarbons; D058428:Silica Gel; D012822:Silicon Dioxide