In vitro monitoring of ciprofloxacin antacids interactions by UV & HPLC.
Ciprofloxacin or 1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl]-3-quinoline carboxylic acid is a fluorinated quinolone antibacterial agent extensively used worldwide. There are number of drug interactions reported for this antibacterial agent. In present studies, in vitro release of ciprofloxacin hydrochloride in presence of various antacids like sodium bicarbonate, calcium hydroxide, calcium carbonate, aluminum hydroxide, magnesium hydroxide, magnesium carbonate, magnesium trisilicate and magaldrate has been studied on BP 2002 dissolution test apparatus. Drug in each case was analyzed either by measuring the absorbance of aliquots at 278 and 316 nm on a UV/VIS spectrophotometer, or by reversed-phase high-performance liquid chromatographic (RP-HPLC) method. These studies were carried out in simulated gastric and intestinal juices for three hours at 37 degrees C. The availability of ciprofloxacin was found to be markedly retarded in presence of all the antacids studied.
Sultana
Najma
N
Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, University of Karachi, Karachi 75270, Pakistan.
Arayne
M Saeed
MS
Hussain
Fida
F
D000327:Adsorption; D000863:Antacids / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000737:chemistry*; D005750:Gastric Juice / Q000737:chemistry; D007700:Kinetics; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
D000863:Antacids; D000900:Anti-Bacterial Agents; D013607:Tablets; D002939:Ciprofloxacin