Antibiotic principles from a Streptomyces species and their sub-acute toxicity studies on hepatic, renal and haemopoietic system of rats.
Two promising antibiotics, JF-A and JF-B were isolated from the chloroform extract of a Banglaeshi Streptomyces strain. The mean zones of inhibition produced by the chloroform extract (400 microg/disc), JF-A (200 microg/disc) and JF-B (200 microg/disc) against 19 pathogenic bacteria were found to be 9-50, 12-38 and 10-41 mm while those produced by a standard antibiotic, kanamycin were 11-40 mm at 30 microg/disc. MICs of JF-A and JF-B were determined to be 64 microg/ml against Bacillus subtilis and 64 and 128 microg/ml against Shigella sonnei, respectively. The extract and the antibiotics were also tested for cytotoxicity against Artemia salina nauplii and LC50 values of 23.26, 18.05 and 32.27 microg/ml were obtained. 90% mortality of shrimp nauplii was observed at 69.18, 50.12 and 110.91 microg/ml, respectively. In a potato disc bioassay, the chloroform extract at 25 microg/disc demonstrated 37.39% inhibition of crown gall tumour induced by G -ve Agrobacterium tumefaciens B6 and the result was statistically significant (P < 0.05). The sub-acute toxicity studies on the JF-A and JF-B reflected innocuous nature of these antibiotics on hepatic, renal and haemopoietic system of rats at 1 mg/kg b.w. on daily administration for 21 consecutive days. This is also confirmed by detailed histopathological studies. No mortality was observed in experimental animals.
Chowdhury
Rasheduzzaman
R
Microbiological Research Laboratory, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh. rzchy@dhaka.net
Musawwir
Farzana
F
Rashid
Rizwana Binte
RB
Mazid
Md Abdul
MA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D001156:Artemia; D001419:Bacteria / Q000187:drug effects; D006413:Hematopoietic System / Q000187:drug effects*; D007668:Kidney / Q000187:drug effects*; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects*; D008297:Male; D051381:Rats; D013302:Streptomyces / Q000378:metabolism
D000900:Anti-Bacterial Agents