Detection of Helicobacter pylori using nested polymerase chain reaction and rapid urease test in gastric biopsy samples.
OBJECTIVE
The purpose of this study was to compare nested polymerase chain reaction and rapid urease test findings in the diagnosis of Helicobacter pylori infection.


METHODS
Two gastric biopsy specimens were obtained from each of 64 patients and polymerase chain reaction and rapid urease test were performed. DNA extraction was followed by amplification with two primer pairs from the urease A gene of Helicobacter pylori genome.


RESULTS
Fourty two patients (65.6%) had a positive rapid urease test result while 22 (34.4%) had a negative result for Helicobacter pylori. Sixty of 64 patients (93.8 %) had a positive result with the nested polymerase chain reaction method. Four patients with negative nested polymerase chain reaction results also had negative rapid urease test results. All of the eighteen patients with rapid urease test-negative results were positive with nested polymerase chain reaction. The number of nested polymerase chain reaction-positive patients (93.8%) was significantly higher than rapid urease test-positive (65.6%) and first round polymerase chain reaction-positive (53.1%) patients (P<0.001).


CONCLUSIONS
Our results indicate that the nested polymerase chain reaction is more specific and sensitive than the rapid urease test for detecting Helicobacter pylori in gastric biopsy samples.
Dönmez-Altuntaş
Hamiyet
H
Erciyes University School of Medicine, Department of Medical Biology, Kayseri. med-biol@erciyes.edu.tr
Güven
Kadri
K