Disappearance of antigenic factor 6 in Candida glabrata IFO 0622 strain cells cultured at high temperature.
The Candida glabrata IFO 0622 strain cells obtained after cultivation at 27 degrees C and at 37 degrees C and then at 27 degrees C (37-27 degrees C) for 48 h in yeast extract-added Sabouraud liquid medium (YSLM) showed the same agglutination patterns against factor sera 1, 4, 6, and 34 in the commercially available factor serum kit 'Candida Check'. On the other hand, the cells of the strain cultured at 37 degrees C had completely lost its reactivity against the factor serum 6. The enzyme-linked immunosorbent assay (ELISA) of the cell wall mannans obtained from the strain cells showed the same reactivity with the agglutination patterns against the factor sera. The 1H-nuclear magnetic resonance (NMR) pattern of the mannan obtained from the strain cells cultured at 37 degrees C showed that the mannan had completely lost the non-reducing beta-1,2-linked mannopyranose unit in the mannotetraose Manbeta1-2Manalpha1-2Manalpha1-2Man, corresponding to the serum factor 6.
Okawa
Yoshio
Y
Second Department of Hygienic Chemistry, Tohoku Pharmaceutical University, Miyagi, Japan. okawa@tohoku-pharm.ac.jp
Goto
Kougi
K
D000371:Agglutination; D000946:Antigens, Fungal / Q000378:metabolism*; D001831:Body Temperature / Q000502:physiology; D041221:Candida glabrata / Q000378:metabolism*; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry
D000946:Antigens, Fungal; D003470:Culture Media; D008351:Mannans