Blood oxidative stress markers are unreliable markers of hepatic steatosis.
BACKGROUND
Non-alcoholic fatty liver disease (NAFLD) and viral hepatitis are associated with hepatic oxidative stress, which is partially dependent on the amount of hepatic fat.


OBJECTIVE
To determine whether the circulating lipid and oxidative stress parameters could be non-invasive markers of hepatic steatosis.


METHODS
Sixty-four patients with NAFLD or viral hepatitis were tested for lipid peroxidation products and antioxidant defence systems, lipid parameters and liver function tests.


RESULTS
Hepatic steatosis was correlated with lipids, gamma-glutamyltranspeptidase, thiobarbituric acid-reactive substances, superoxide dismutase and superoxide dismutase/erythrocyte glutathione peroxidase ratio. gamma-Glutamyltranspeptidase, triglycerides and low-density lipoprotein cholesterol were significantly higher in the presence of steatosis. No difference in blood oxidative stress markers was observed according to the presence or absence of steatosis except for the superoxide dismutase/erythrocyte glutathione peroxidase ratio. Total cholesterol, triglycerides and low-density lipoprotein cholesterol were significantly higher in the NAFLD group (n = 17, 60% mean steatosis grade) than in the viral hepatitis group (n = 20, 13% mean steatosis grade). Only superoxide dismutase was lower and vitamin E higher in NAFLD than in viral hepatitis patients.


CONCLUSIONS
Standard blood oxidative stress markers do not predict the extent of hepatic steatosis as they probably do not accurately reflect intrahepatic oxidative stress. Serum lipid levels were best correlated with hepatic steatosis.
Bonnefont-Rousselot
D
D
Laboratoire des Lipides, Groupe Hosptalier Pitie-Salpetriere (AP-HP), Paris, France. dominique.rousselot@psl.aphp.fr
Ratziu
V
V
Giral
P
P
Charlotte
F
F
Beucler
I
I
Poynard
T
T
D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D002784:Cholesterol / Q000097:blood; D005234:Fatty Liver / Q000378:metabolism*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D019698:Hepatitis C, Chronic / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012643:Selenium / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D014810:Vitamin E / Q000097:blood
D015415:Biomarkers; D014810:Vitamin E; D002784:Cholesterol; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium