Quantitative determination of beta-asarone in calamus by high-performance thin-layer chromatography.
A quantitative high-performance thin-layer chromatographic method for determination of beta-asarone in Calamus rhizome was developed and validated. The method is suitable for proper identification of Acorus calamus. Through the use of caffeine-modified silica gel as the stationary phase and toluene-ethyl acetate (93 + 7, v/v) as the mobile phase, beta-asarone is baseline separated from its isomer alpha-asarone. Scanning densitometry with absorption measurement at 313 nm allows specific, accurate, and precise quantification of beta-asarone. The working range of 40 to 200 ng absolute of the target substance is sufficient to establish whether a given sample passes the limit test of 0.5% maximum as required by the Swiss Pharmacopoeia.
Widmer
Valeria
V
CAMAG Laboratory Services, Muttenz, Switzerland.
Schibli
Anne
A
Reich
Eike
E
D000081248:Allylbenzene Derivatives; D000840:Anisoles / Q000032:analysis*; D031081:Calamus / Q000737:chemistry*; D002855:Chromatography, Thin Layer / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000081248:Allylbenzene Derivatives; D000840:Anisoles; C012195:asarone