title: Genome sequence of the thermostable Newcastle disease virus (strain I-2) reveals a possible phenotypic locus.
abstract: The complete genome sequence of the Australian I-2 heat-tolerant Newcastle disease virus (NDV) vaccine (master seed stocks) was determined and compared to the sequence of the parent virus from which it had been derived after exposure of the parent stock at 56 degrees C for 30 min. Nucleotide changes were observed at a number of positions with synonymous mutations being greater than those observed for non-synonymous mutations. Sequence data for the HN gene of a parental culture of V4 and two heat-tolerant variants of V4 were obtained. These were compared with the data for the I-2 viruses and with published sequences for parental V4 and for a number of ND vaccine strains. Sequence analyses did not reveal the ARG(303) deletion in the HN protein, previously claimed to be responsible for the thermostable phenotype. No consistent changes were detected that would indicate involvement of the HN protein in heat resistance. The majority of alterations were observed in the L protein of the virus and it is proposed that these alterations were responsible for the heat-tolerant phenotype of the I-2 NDV vaccine.
authors.affiliation: CSIRO Livestock Industries, Australian Animal Health Laboratory, P.O. Bag 24, Geelong 3220, Australia. j.kattenbelt@uq.edu.au
authors.forename: J A
authors.initials: JA
authors.lastname: Kattenbelt
authors.forename: J
authors.initials: J
authors.lastname: Meers
authors.forename: A R
authors.initials: AR
authors.lastname: Gould
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D017931:DNA Primers / Q000737:chemistry; D014644:Genetic Variation / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D015707:HN Protein / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000517:prevention & control; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D011201:Poultry Diseases / Q000517:prevention & control / Q000821:virology; D016415:Sequence Alignment; D014765:Viral Vaccines / Q000737:chemistry / Q000235:genetics
chemical_list: D017931:DNA Primers; D015707:HN Protein; D014765:Viral Vaccines