[Induction of EIAV-specific cellular immune response by attenuated EIAV vaccine].
OBJECTIVE
To elucidate cellular immune protective mechanism of EIAV.


METHODS
Four horses were immunized with (DLV) by subcutaneous injection and 2 horses with 0.85% sodium chloride as the negative control. Rectal temperatures and clinical features were recorded daily. Whole blood samples were collected, from which PBMC were separated and used for CTL assay and lymphocyte proliferation assay.


RESULTS
The target cells were activated by PWM and treated with DLV or recombinant vaccinia vectors expressing DLV and LN Gag or Env separately. The percentage of EIAV-specific CTL lysis was under 5% in the negative control group, however, that was 20% to 30% in the group of immunized horses. The stimulation index (SI) from proliferation assay was 3 in the group immunized with attenuated DLV vaccine.


CONCLUSIONS
The results indicated that EIAV-specific cellular immune-response induced by DLV immunization may contribute to the immune protective effect against EIAV virus infection.
Zhang
Xiao-yan
XY
National Center for AIDS/STD Control and Prevention, China CDC, Beijing 100050, China. zhangxy@chinaids.org.cn
Li
Hong-mei
HM
Liang
Hua
H
Shen
Tao
T
Ma
Yan
Y
Xiang
Wen-hua
WH
Shen
Rong-xian
RX
Shao
Yi-ming
YM
D000818:Animals; D006736:Horses; D007111:Immunity, Cellular / Q000276:immunology*; D004860:Infectious Anemia Virus, Equine / Q000276:immunology*; D007267:Injections; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D014613:Vaccines, Attenuated; D014765:Viral Vaccines