Transcriptional regulation of the Neurospora circadian clock gene wc-1 affects the phase of circadian output.
WHITE COLLAR-1 (WC-1) is the limiting component of the White Collar Complex (WCC) controlling expression of the Neurospora circadian clock protein Frequency (FRQ). Accumulation of WC-1 is supported by FRQ on a post-transcriptional level. Here, we show that transcription of wc-1 is organized in a complex way. Three promoters drive transcription of wc-1. Pdist is dependent on WCC. Pprox is independent of WCC in darkness, but inducible by light in a WCC-dependent manner. A third promoter, Pint, is located in the wc-1 open reading frame and promotes expression of an amino-terminally truncated WC-1 isoform of unknown function. Expression of wc-1 by Pdist or Pprox alone, or by a heterologous promoter, affects the entrained phase of circadian conidiation and the response of Neurospora to light. Our results indicate that transcriptional regulation of wc-1 is required to modulate the circadian phase of clock output.
Káldi
Krisztina
K
Biochemie-Zentrum der Universität Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.
González
Beatriz Herreros
BH
Brunner
Michael
M
D000595:Amino Acid Sequence; D001683:Biological Clocks; D015153:Blotting, Western; D002940:Circadian Rhythm / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D025461:Feedback, Physiological; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D008156:Luciferases / Q000378:metabolism; D008969:Molecular Sequence Data; D009491:Neurospora / Q000235:genetics* / Q000502:physiology*; D012331:RNA, Fungal / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; C087405:FRQ protein, Neurospora crassa; D005656:Fungal Proteins; D012331:RNA, Fungal; D014157:Transcription Factors; C105589:wc-1 protein, Neurospora crassa; D008156:Luciferases