Human cytomegalovirus immediate-early 2 protein IE86 blocks virus-induced chemokine expression.
The effect of human cytomegalovirus (HCMV) gene expression on cytokine (beta interferon) and chemokine (RANTES, MIG, MCP-2, MIP-1alpha, and interleukin-8) expression was examined. We demonstrate that HCMV gene expression is required to suppress the transcriptional induction of these cytokines and that the HCMV immediate-early 2 gene product IE86 can effectively block the expression of cytokines and proinflammatory chemokines during HCMV and Sendai virus infection. Additionally, we present data on viral mutants and ectopic protein expression which demonstrate that pp65, another identified HCMV cytokine antagonist, is not involved in regulating these proinflammatory cytokines. This is the first report to demonstrate that IE86 can act to suppress virus-induced proinflammatory cytokine transcript expression, extending the antiviral properties of this multifunctional viral protein.
Taylor
R Travis
RT
Department of Microbiology, University of Minnesota, 1060 Mayo Building, MMC196, Minneapolis, MN 55455, USA.
Bresnahan
Wade A
WA
D002478:Cells, Cultured; D018925:Chemokines / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000276:immunology / Q000821:virology*; D015870:Gene Expression; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D007113:Immunity, Innate; D009154:Mutation; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D018925:Chemokines; C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D015534:Trans-Activators