Treatment with Met-RANTES decreases bacterial translocation in experimental colitis.
BACKGROUND
During colitis, epithelial function is impaired, leading to increased bacterial translocation. Recent studies have shown the important role of proinflammatory cytokines and chemokines, including RANTES (regulated on activation, normal T-cell expressed and secreted), in inflammatory bowel diseases (IBDs). In this study, we evaluated the role of Met-RANTES, an antagonist of the RANTES receptor, on the impairment of bacterial translocation in a rat model of colitis.


METHODS
Rats were randomly assigned to 3 groups. Group 1 = control, group 2 = experimental colitis, and group 3 = colitis plus Met-RANTES treatment. On day 7 after colitis was induced, plasma tumor necrosis factor-alpha colon tissue myeloperoxidase and portal blood endotoxin levels were measured. Lymph node, liver, and spleen culture quantified bacterial translocation.


RESULTS
Met-RANTES treatment resulted in significant decreases in colonic damage as well as bacterial translocation in experimental colitis.


CONCLUSIONS
These results suggest that chemokine receptor antagonists may potentially be useful in the treatment of IBDs.
Kucuk
Can
C
Department of General Surgery, Erciyes University School of Medicine, 38039, Kayseri, Turkey. cankucuk@erciyes.edu.tr
Sozuer
Erdogan
E
Gursoy
Sebnem
S
Canoz
Ozlem
O
Artis
Tarik
T
Akcan
Alper
A
Akyildiz
Hizir
H
Muhtaroglu
Sebahattin
S
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D018988:Bacterial Translocation / Q000187:drug effects*; D018946:Chemokine CCL5 / Q000031:analogs & derivatives* / Q000494:pharmacology; D003092:Colitis / Q000139:chemically induced / Q000382:microbiology / Q000503:physiopathology*; D003106:Colon / Q000187:drug effects*; D004195:Disease Models, Animal; D008297:Male; D009676:Noxae / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects
D000893:Anti-Inflammatory Agents; D018946:Chemokine CCL5; D009676:Noxae; C108335:RANTES, Met-; D014302:Trinitrobenzenesulfonic Acid