[Effects of dahuang zhechong pill on expression and activity of matrix metalloproteinase in rats' hepatic stellate cells].
OBJECTIVE
To observe the effects of Dahuang Zhechong Pill (DZP) on the gene expression of matrix metalloproteinase-1 (MMP-1) in rats' hepatic stellate cells (HSC) and activity of matrix metalloproteinase-2 (MMP-2) that secreted into the culture base.


METHODS
HSC were isolated from the liver of normal rats and incubated with DZP-contained drug serum. The expression of MMP-1 in the HSC was detected by quantitative reverse-transcription polymerase chain reaction (RT-PCR). The activity of MMP-2 was examined by zymography.


RESULTS
DZP-contained drug serum could obviously promote the gene expression of MMP-1 in HSC. In the meantime, it could obviously increase the content and activity of MMP-2 synthesized by HSC (P < 0.05).


CONCLUSIONS
The anti-fibrosis action of DZP was correlated to the promotion of HSC's gene expression of MMP-1 and increasing of the contents and activity of MMP-2.
Pan
Zhi-heng
ZH
Department of Traditional Chinese Medicine, The Third Affiliated Hospital of Zhongshan University, Guangzhou 510630. stevenp2@21cn.com
Xie
Yao
Y
He
Hong-wen
HW
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
C521634:Dahuang Zhechong pill; D004365:Drugs, Chinese Herbal; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1