A general two-step strategy to synthesize lariat RNAs.
We describe a general and efficient two-step strategy for lariat RNA synthesis. In the first step, a deoxyribozyme synthesizes 2',5'-branched RNA. In the second step, T4 RNA ligase closes the loop that completes the lariat. The loop-closure reaction can form either a natural or unnatural lariat isomer, depending on which of the two 3'-termini of the branched RNA reacts with the lone 5'-end. We demonstrate two approaches to control formation of either lariat isomer. In conjunction with other routes for lariat RNA synthesis, the two-step strategy described here will facilitate biochemical studies that require lariat RNAs of varying nucleotide sequence.
Wang
Yangming
Y
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA.
Silverman
Scott K
SK
D001483:Base Sequence; D001671:Biochemistry / Q000379:methods*; D007438:Introns; D008969:Molecular Sequence Data; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry; D011130:RNA Ligase (ATP) / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing; D000079962:RNA, Circular
D000079962:RNA, Circular; D012313:RNA; D011130:RNA Ligase (ATP)