title: [Induction of EIAV-specific cellular immune response by attenuated EIAV vaccine].
abstract: OBJECTIVE
  To elucidate cellular immune protective mechanism of EIAV.
  
  
  METHODS
  Four horses were immunized with (DLV) by subcutaneous injection and 2 horses with 0.85% sodium chloride as the negative control. Rectal temperatures and clinical features were recorded daily. Whole blood samples were collected, from which PBMC were separated and used for CTL assay and lymphocyte proliferation assay.
  
  
  RESULTS
  The target cells were activated by PWM and treated with DLV or recombinant vaccinia vectors expressing DLV and LN Gag or Env separately. The percentage of EIAV-specific CTL lysis was under 5% in the negative control group, however, that was 20% to 30% in the group of immunized horses. The stimulation index (SI) from proliferation assay was 3 in the group immunized with attenuated DLV vaccine.
  
  
  CONCLUSIONS
  The results indicated that EIAV-specific cellular immune-response induced by DLV immunization may contribute to the immune protective effect against EIAV virus infection.
authors.affiliation: National Center for AIDS/STD Control and Prevention, China CDC, Beijing 100050, China. zhangxy@chinaids.org.cn
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Zhang
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Liang
authors.forename: Tao
authors.initials: T
authors.lastname: Shen
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Xiang
authors.forename: Rong-xian
authors.initials: RX
authors.lastname: Shen
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Shao
mesh_terms: D000818:Animals; D006736:Horses; D007111:Immunity, Cellular / Q000276:immunology*; D004860:Infectious Anemia Virus, Equine / Q000276:immunology*; D007267:Injections; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines