Effect of interleukin-8 on histamine release from totally isolated vascularly perfused rat stomach.
BACKGROUND
Recent studies have demonstrated relationships between cytokines and gastric acid secretion. The present study was performed in rats to elucidate the effects of interleukin-8 (IL-8) on gastric acid secretion through an increase in histamine release from the stomach.


METHODS
The experiments were performed in gastric lumen-perfused rats for the study of acid secretion and in totally isolated vascularly perfused rat stomach preparations for the study of histamine release. The histamine in the effluent was determined by radioimmunoassay.


RESULTS
IL-8 (500 ng) significantly enhanced gastrin-stimulated acid secretion. IL-8, at a concentration of 500 ng/20 ml per 10 min, did not alter basal histamine release, but at 100 ng/20 ml and 500 ng/20 ml it dose-dependently increased gastrin-stimulated histamine release.


CONCLUSIONS
IL-8 enhances gastrin-stimulated acid secretion and histamine release from the rat stomach, which may explain the enhancing effect of IL-8 on gastric acid secretion.
Yakabi
Koji
K
The First Department of Internal Medicine, Saitama Medical Center, Saitama Medical School, 1981 Kamoda Tsujido-machi, Kawagoe 350-8550, Japan.
Ro
Shoki
S
Miura
Seiichi
S
Tanaka
Teruyuki
T
Ohno
Shino
S
Kawashima
Junichi
J
Kurosawa
Susumu
S
Nakamura
Takashi
T
D000818:Animals; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D005755:Gastrins / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects / Q000502:physiology*; D016209:Interleukin-8 / Q000494:pharmacology / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005755:Gastrins; D016209:Interleukin-8