title: Induction of immune responses against glycosphingolipid antigens: comparison of antibody responses in mice immunized with antigen associated with liposomes prepared from various phospholipids.
abstract: The immune responses of mice against glycosphingolipid (GSL) antigens and the effect of the phospholipid composition of liposomes on the immunogenicity in mice of liposome-associated GSL antigens were examined. The immunization with GSL antigen alone was unable to induce any detectable anti-GSL antibody responses. On the other hand, the immune responses against GSL antigens were detected after immunization with liposomes composed of dipalmitoylphosphatidylcholine (DPPC) (0.5 micromol), cholesterol (Chol) (0.5 micromol), Salmonella minnesota R595 lipopolysaccharides (LPS) (10 microg) and GSL (0.05 micromol) (DPPC-liposome). However, the administration with liposome composed of dimyristoylphosphatidylcholine (DMPC) (0.5 micromol), Chol (0.5 micromol), S. minnesota R595 LPS (10 microg) and GSL (0.05 micromol) and with liposomes composed of distearylphosphatidylcholine (DSPC) (0.5 micromol), Chol (0.5 micromol), and S. minnesota R595 LPS (10 microg) and GSL (0.05 micromol) was ineffective for the induction of the immune responses against GSL antigens. These results suggest that DPPC-liposome would serve effectively as a delivery vehicle for inducing immune responses against GSL antigen.
authors.affiliation: Laboratory of Veterinary Immunology, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Uemura
authors.forename: Shinobu
authors.initials: S
authors.lastname: Watarai
authors.forename: Tadashi
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kodama
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000008:administration & dosage* / Q000276:immunology; D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D000954:Antigens, Surface / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D005732:Gangliosides / Q000008:administration & dosage* / Q000276:immunology; D006028:Glycosphingolipids / Q000276:immunology*; D008070:Lipopolysaccharides / Q000008:administration & dosage* / Q000276:immunology; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D010713:Phosphatidylcholines / Q000008:administration & dosage* / Q000276:immunology; D013050:Spectrometry, Fluorescence
chemical_list: D000954:Antigens, Surface; D005732:Gangliosides; D006028:Glycosphingolipids; D008070:Lipopolysaccharides; D008081:Liposomes; D010713:Phosphatidylcholines; C038777:chol-1 antigen; D015060:1,2-Dipalmitoylphosphatidylcholine