title: Linkage mapping of a dominant male sterility gene Ms-cd1 in Brassica oleracea.
abstract: The dominant male sterility gene Ms-cd1 (c, cabbage; d, dominant) was identified as a spontaneous mutation in the spring cabbage line 79-399-3. The Ms-cd1 gene is successfully applied in hybrid seed production of several Brassica oleracea cultivars in China. Amplified fragment length polymorphism (AFLP) technology was used to identify markers linked to the Ms-cd1 gene in bulks of male-sterile and male-fertile individuals of a segregating BC3 population and in a near-isogenic population of 25 male-sterile plants. Twelve markers within a 20-cM interval proximal to the Ms-cd1 gene were identified, 5 of which can be used to select homozygous male-sterile Ms-cd1/ Ms-cd1 plants. Three AFLP markers and 3 sequence characterized amplified region markers that were linked to MS-cd1 mapped onto linkage group O9, corresponding to chromosome 3 of B. oleracea. This region corresponds to the top of chromosome 5 in Arabidopsis thaliana.
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. wangxw@mail.caas.net.cn
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Lou
authors.forename: Guusje
authors.initials: G
authors.lastname: Bonnema
authors.forename: Baojun
authors.initials: B
authors.lastname: Yang
authors.forename: Hangjun
authors.initials: H
authors.lastname: He
authors.forename: Yanguo
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Fang
mesh_terms: D001937:Brassica / Q000235:genetics*; D002874:Chromosome Mapping; D005799:Genes, Dominant; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers; D006720:Homozygote; D051479:Plant Infertility / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D005819:Genetic Markers