title: Determination of five coumarins in Cnidii Fructus by micellar electrokinetic capillary chromatography.
abstract: A micellar electrokinetic capillary chromatography (MEKC) method for the separation and quantification of the coumarins in Cnidii Fructus was developed for the first time. Within 25 minutes, 5 major coumarins were separated using 18 mM borate, 12 mM phosphate and 50 mM SDS (pH 9.2) containing 20 % methanol. The relative standard deviations of migration times and peak areas were less than 5 %. The total contents of the five coumarins in plants from different habitats ranged from 13.6 to 44.6 mg/g, and recoveries ranged from 96.0 to 102.8 %. The effects of buffer concentration, SDS, pH value and organic modifier on the separation were investigated.
authors.affiliation: Key Laboratory of Modern Chinese Medicine, Ministry of Education, Nanjing, P.R. China.
authors.forename: Meng Hua
authors.initials: MH
authors.lastname: Wu
authors.forename: Lu Hua
authors.initials: LH
authors.lastname: Zhao
authors.forename: Yue
authors.initials: Y
authors.lastname: Song
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Bing Ren
authors.initials: BR
authors.lastname: Xiang
authors.forename: Ling Hua
authors.initials: LH
authors.lastname: Mei
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary; D031048:Cnidium / Q000737:chemistry*; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D003374:Coumarins