title: Antibody pretargeting advances cancer radioimmunodetection and radioimmunotherapy.
abstract: This article reviews the methods of pretargeting, which involve separating the targeting antibody from the subsequent delivery of an imaging or therapeutic agent that binds to the tumor-localized antibody. This provides enhanced tumor:background ratios and the delivery of a higher therapeutic dose than when antibodies are directly conjugated with radionuclides, as currently practiced in cancer radioimmunotherapy. We describe initial promising clinical results using streptavidin-antibody constructs with biotin-radionuclide conjugates in the treatment of patients with malignant gliomas, and of bispecific antibodies with hapten-radionuclides in the therapy of tumors expressing carcinoembryonic antigen, such as medullary thyroid and small-cell lung cancers.
authors.affiliation: Garden State Cancer Center, Center for Molecular Medicine and Immunology, Belleville, NJ 07109, USA. dmg.gscancer@att.net
authors.forename: David M
authors.initials: DM
authors.lastname: Goldenberg
authors.forename: Robert M
authors.initials: RM
authors.lastname: Sharkey
authors.forename: Giovanni
authors.initials: G
authors.lastname: Paganelli
authors.forename: Jacques
authors.initials: J
authors.lastname: Barbet
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Chatal
mesh_terms: D000912:Antibodies, Neoplasm / Q000276:immunology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000000981:diagnostic imaging / Q000628:therapy; D002986:Clinical Trials as Topic; D005910:Glioma / Q000000981:diagnostic imaging / Q000628:therapy; D006241:Haptens; D006801:Humans; D007202:Indicators and Reagents; D008175:Lung Neoplasms / Q000000981:diagnostic imaging / Q000628:therapy; D016719:Radioimmunodetection / Q000379:methods*; D016499:Radioimmunotherapy / Q000379:methods*; D019809:Streptavidin; D013964:Thyroid Neoplasms / Q000000981:diagnostic imaging / Q000628:therapy
chemical_list: D000912:Antibodies, Neoplasm; D006241:Haptens; D007202:Indicators and Reagents; D019809:Streptavidin