[Effects of enamel matrix proteins on the attachment,spreading and proliferation of porcine bone marrow stromal cells in vitro].
OBJECTIVE
The purpose of this study is to investigate the influence of enamel matrix proteins(EMPs) on the attachment, spreading and proliferation of cultured porcine bone marrow stromal cells (BMSCs).


METHODS
BMSCs were obtained from porcine bone marrow aspiration and cultured in DMEM medium with 10% FBS.The third passage cells were exposed to various concentrations of EMPs (25,50,100 and 200microg /ml).Controls were BMSCs cultured in DMEM medium without EMPs. The cell attachment was analyzed by a colorimetric assay and cell spreading rates were performed at culture time of 1h, 3.5h and 6.5h by analysis of micrographs taken at predetermined sites of each wells. BMSCs proliferation rates were carried out over a 9-day period and assessed by an MTT assay. A parametric one-way analysis of variance (ANOVA) and multiple comparisons were used to test the treatments based on the Stument-Newman- Keuls test.


RESULTS
It was shown that BMSCs well attached and spread on EMPs-coated substrata,however, there were no significant differences between the control group and experimental groups with various concentrations of EMPs. The proliferation of BMSCs was significantly stimulated by EMPs in a dose- and time- dependent manner,and EMPs at a concentration of 200microg/ml significantly enhanced BMSCs proliferation from day 3 over the experiment.


CONCLUSIONS
The results demonstrate that EMPs stimulate BMSCs proliferation, whereas this factor does not affect the attachment and spreading of these cells. These findings lend theoretical support to the combined application of EMPs and BMSCs in repairing periodontal defects.
Song
Ai-mei
AM
Department of Oral Medicine, School of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Shu
Rong
R
Jiang
Xin-quan
XQ
Zhang
Xiu-li
XL
Liu
Xiao-feng
XF
Zhang
Wen
W
D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D013552:Swine
D003746:Dental Enamel Proteins; C109069:enamel matrix proteins