title: A fibronectin-binding protein from Streptococcus equi binds collagen and modulates cell-mediated collagen gel contraction.
abstract: The N-terminal fragment (FNZN) of the fibronectin-binding protein FNZ from Streptococcus equi subspecies zooepidemicus was investigated as to effects on murine cell interactions with extracellular matrix proteins. FNZN bound to immobilized fibronectin (FN) and native, but not denatured, collagen type I. FNZN had no effect on primary adhesion of cells from the murine myoblastic C2C12 cell line to immobilized fibronectin. C2C12 cells adhered to immobilized FNZN, a process that was not inhibited by anti-human FN IgG or by an inhibitor of integrin alphaVbeta3. C2C12 cells lack collagen-binding beta1 integrins and neither adhere to native collagen nor mediate contraction of three-dimensional collagen gels. FNZN stimulated collagen gel contraction by C2C12 cells but not adhesion of C2C12 cells to collagen. Experiments with an alphaVbeta3-inhibitor suggested that FNZN promoted contraction by a process requiring alphaVbeta3. Our data suggest that FNZN by binding to cells, collagen, and FN modulate complex adhesive processes mediated by the alphaVbeta3 integrin. Since alphaVbeta3-mediated contractile events function to counteract edema formation during inflammation, it is possible that FNZN and its secreted homologue FNE modulate edema responses in infected tissues.
authors.affiliation: Department of Medical Biochemistry and Microbiology, University of Uppsala, BMC Box 582, SE-751 23 Uppsala, Sweden.
authors.forename: Asa
authors.initials: A
authors.lastname: Lidén
authors.forename: Asa
authors.initials: A
authors.lastname: Karlström
authors.forename: Jonas
authors.initials: J
authors.lastname: Lannergård
authors.forename: Sebastian
authors.initials: S
authors.lastname: Kalamajski
authors.forename: Bengt
authors.initials: B
authors.lastname: Guss
authors.forename: Kristofer
authors.initials: K
authors.lastname: Rubin
authors.forename: Cecilia
authors.initials: C
authors.lastname: Rydén
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D003094:Collagen / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005782:Gels / Q000378:metabolism; D006801:Humans; D051379:Mice; D032446:Myoblasts / Q000378:metabolism; D018502:Streptococcus equi / Q000737:chemistry / Q000378:metabolism*; D044366:Transition Temperature
chemical_list: D018829:Adhesins, Bacterial; D016326:Extracellular Matrix Proteins; D005782:Gels; C078635:fibronectin-binding proteins, bacterial; D003094:Collagen