Microviscoelastic moduli of biomimetic cell envelopes.
Bioanalogue models of composite cell envelopes were designed by electrostatically driven self-assembly of actin shells inside giant vesicles. Viscoelastic relaxation moduli were measured between 0.03 and 20 s as a function of actin density by magnetic bead microrheometry. The shear relaxation spectra exhibited by the composite shells compare well with those of natural cell envelopes and bulk entangled actin networks. Absolute value of the shear modulus was measured for the first time by deformation field mapping. Shear and bending moduli agree well with values obtained by bead fluctuations analysis.
Limozin
Laurent
L
E22-Biophysik. Physik Department, Technical University of Munich, James Franck Strasse, D-85748 Garching, Germany. limozin@marseille.inserm.fr
Roth
Alexander
A
Sackmann
Erich
E
D000199:Actins / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry; D004548:Elasticity; D008051:Lipid Bilayers / Q000737:chemistry*; D008567:Membranes, Artificial; D014783:Viscosity
D000199:Actins; D008051:Lipid Bilayers; D008567:Membranes, Artificial