title: A proteomics approach to identify long-term molecular changes in rat medial prefrontal cortex resulting from sucrose self-administration.
abstract: The medial prefrontal cortex (mPFC) is involved in the processing and retrieval of reward-related information. Here, we investigated long-lasting changes in protein composition of the mPFC in rats with a history of sucrose self-administration. Protein levels were analyzed using 2-D PAGE and MALDI-TOF sequencing. From approximately 1500 spots, 28 regulated proteins were unambiguously identified and were involved in cytoskeleton organization, energy metabolism, oxidative stress, neurotransmission, and neuronal outgrowth and differentiation. For several proteins, this change was also found as a long-lasting alteration in gene expression. We show that self-administration of sucrose produces long-lasting molecular neuroadaptations in the mPFC that may be involved in reward-related information processing.
authors.affiliation: Department of Molecular and Cellular Neurobiology, Center for Neurogenomics & Cognitive Research, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
authors.forename: Michel C
authors.initials: MC
authors.lastname: Van den Oever
authors.forename: Sabine
authors.initials: S
authors.lastname: Spijker
authors.forename: Ka Wan
authors.initials: KW
authors.lastname: Li
authors.forename: Connie R
authors.initials: CR
authors.lastname: Jiménez
authors.forename: Eisuke
authors.initials: E
authors.lastname: Koya
authors.forename: Roel C
authors.initials: RC
authors.lastname: Van der Schors
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Gouwenberg
authors.forename: Rob
authors.initials: R
authors.lastname: Binnekade
authors.forename: Taco J
authors.initials: TJ
authors.lastname: De Vries
authors.forename: Anton N M
authors.initials: AN
authors.lastname: Schoffelmeer
authors.forename: August B
authors.initials: AB
authors.lastname: Smit
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D008297:Male; D017397:Prefrontal Cortex / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D012646:Self Administration; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013395:Sucrose / Q000008:administration & dosage*
chemical_list: D011506:Proteins; D013395:Sucrose