Growth in Stewart's medium is a simple, rapid and inexpensive screening tool for the identification of Burkholderia cepacia complex.
Ninety-one percent of Burkholderia cepacia complex reference strains (66 out of 72) displayed a yellow slope-green butt colour reaction after growth in Stewart's medium indicating the oxidation of glucose and the absence of an arginine dihydrolase system. This same colour reaction was observed for Burkholderia gladioli and several Ralstonia species, but not for Pseudomonas aeruginosa, Stenotrophomonas, Achromobacter, Pandoraea and several other Gram-negative non-fermenting bacilli. We therefore consider growth in Stewart's medium a useful, simple, rapid and inexpensive screening test to reduce the number of false positive isolates from B. cepacia complex selective media.
Vanlaere
Elke
E
Laboratory of Microbiology, Gent University, 9000 Gent, Belgium.
Hansraj
Faiza
F
Vandamme
Peter A R
PA
Govan
John R W
JR
D015373:Bacterial Typing Techniques; D019121:Burkholderia Infections / Q000382:microbiology*; D042602:Burkholderia cepacia complex / Q000145:classification* / Q000254:growth & development; D003470:Culture Media; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D007202:Indicators and Reagents
D003470:Culture Media; D007202:Indicators and Reagents