Determination of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) using LC-ESI-MS method and application to hemolyzed blood.
Rapid and reliable methods for identification of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) in antiseptic and hemolyzed blood using electrospray ionization mass spectrometry (ESI-MS) were developed. Fragmental analysis provides accurate evidence for the presence of CHD in the samples. For the determination of CHD in hemolyzed blood, the method was also developed using LC-ESI-MS. Linearity of calibration curve was obtained over the concentration range of 0.1-11 microg/mL with residuals from -4.3 to 6.7%. We applied the methods to the case of suicidal injection of antiseptic and successfully detected CHD and NPEOn from hemolyzed blood. The CHD concentration was 352 microg/mL.
Usui
Kiyotaka
K
Division of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
Hishinuma
Takanori
T
Yamaguchi
Hiroaki
H
Tachiiri
Naoki
N
Goto
Junichi
J
D000328:Adult; D000891:Anti-Infective Agents, Local / Q000097:blood; D002710:Chlorhexidine / Q000097:blood* / Q000506:poisoning; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005026:Ethylene Glycols / Q000097:blood* / Q000506:poisoning; D005260:Female; D006461:Hemolysis; D006801:Humans; D007262:Infusions, Intravenous; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013405:Suicide; D013501:Surface-Active Agents / Q000032:analysis
D000891:Anti-Infective Agents, Local; D005026:Ethylene Glycols; D013501:Surface-Active Agents; C021754:terics; D002710:Chlorhexidine