title: Immunolocalization of a candidate pheromone receptor in the antenna of the male moth, Heliothis virescens.
abstract: Pheromone recognition in insects is thought to involve distinct receptor proteins in the dendritic membrane of antennal sensory neurons. We have generated antibodies directed against a peptide derived from the sequence of the candidate pheromone receptor HR13 from Heliothis virescens. The antibodies specifically labelled the cell bodies of a distinct neuron population housed in male-specific pheromone-sensitive sensilla. Combining antibody staining with in situ hybridization the reactive cells were found to express the HR13 gene. In addition, dendrites projecting into sensilla hairs as well as the axonal processes of immunoreactive cells were labelled. Labelling of axons has allowed visualization of their fasciculation within antennal segments and permits tracking of axons as they merge into the antennal nerve. The HR13 protein was first detected 1 day before eclosion. Thus, the distribution of HR13 protein in the antennal neurons of the male moth strongly suggests a role of the HR13 receptor in recognition of pheromones.
authors.affiliation: Institute of Physiology (230), University of Hohenheim, Garbenstrasse 30, 70599, Stuttgart, Germany
authors.forename: Thomas
authors.initials: T
authors.lastname: Gohl
authors.forename: Jürgen
authors.initials: J
authors.lastname: Krieger
mesh_terms: D000818:Animals; D005260:Female; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D018613:Microscopy, Confocal; D009036:Moths / Q000502:physiology*; D009434:Neural Pathways / Q000648:ultrastructure*; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D043563:Receptors, Pheromone / Q000235:genetics / Q000378:metabolism*; D012726:Sexual Behavior, Animal / Q000502:physiology
chemical_list: D043563:Receptors, Pheromone