title: Autorepression of RctB, an initiator of Vibrio cholerae chromosome II replication.
abstract: The RctB protein binds to the origin of replication of Vibrio cholerae chromosome II (chrII) and is required for oriCIIVc-based replication. Here, we found that RctB acts as an autorepressor, inhibiting rctB transcription. Integration host factor promotes rctB transcription, while Dam and DnaA, factors required for replication of both V. cholerae chromosomes, influence RctB autorepression. Thus, RctB appears to regulate chrII replication as both an initiator and a transcription repressor, and its synthesis is modulated by factors that govern replication of both chromosomes.
authors.affiliation: Genetics Program and Department of Molecular Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Ave., Boston, MA 02111, USA.
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Egan
authors.forename: Stephane
authors.initials: S
authors.lastname: Duigou
authors.forename: Matthew K
authors.initials: MK
authors.lastname: Waldor
mesh_terms: D002876:Chromosomes, Bacterial / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D018741:Replication Origin; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors; D024363:Transcription Initiation Site; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: D012097:Repressor Proteins; D014157:Transcription Factors