title: Heterologous expression of an Entamoeba histolytica chitin synthase in Saccharomyces cerevisiae.
abstract: Chitin in the cyst wall of Entamoeba histolytica is made by two chitin synthases (Chs), one of which is unique (EhCHS-1) and one of which resembles those of insects and nematodes (EhCHS-2). EhCHS-1 is deposited chitin in the lateral wall of transformed Saccharomyces cerevisiae Chs mutants, independent of accessory proteins (Chs4p to Chs7p) required by yeast Chs3p.
authors.affiliation: Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 715 Albany St., Evans 426, Boston, Massachusetts 02118, USA.
authors.forename: Katrina L
authors.initials: KL
authors.lastname: Van Dellen
authors.forename: Dorota A
authors.initials: DA
authors.lastname: Bulik
authors.forename: Charles A
authors.initials: CA
authors.lastname: Specht
authors.forename: Phillips W
authors.initials: PW
authors.lastname: Robbins
authors.forename: John C
authors.initials: JC
authors.lastname: Samuelson
mesh_terms: D000818:Animals; D002384:Catalysis; D002686:Chitin / Q000378:metabolism; D002687:Chitin Synthase / Q000235:genetics* / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression; D009154:Mutation / Q000235:genetics; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D002686:Chitin; D002687:Chitin Synthase