Hepatoprotective constituents of the edible brown alga Ecklonia stolonifera on tacrine-induced cytotoxicity in Hep G2 cells.
In this study, ethanolic extracts from 18 seaweed variants were assessed for hepatoprotective activity against tacrine-induced cytotoxicity in Hep G2 cells. Only one of these, Ecklonia stolonifera Okamura (Laminariaceae), a member of the brown algae, exhibited promising hepatoprotective activity. Bioassay-guided fractionation of the active ethyl acetate (EtOAc) soluble fraction obtained from the ethanolic extract of E. stolonifera, resulted in the isolation of several phlorotannins [phloroglucinol (1), eckstolonol (2), eckol (3), phlorofucofuroeckol A (4), and dieckol (5)]. Compounds 2 and 4 were determined to protect Hep G2 cells against the cytotoxic effects of tacrine, with EC50 values of 62.0 and 79.2 microg/mL, respectively. Silybin, a well characterized hepatoprotective agent, was used as a positive control, and exhibited an EC50 value of 50.0 microg/mL. It has been suggested that the phlorotannins derived from marine brown algae might prove useful sources in the development of novel hepatoprotective agents.
Kim
Youn Chul
YC
College of Pharmacy, Wonkwang University, Iksan, Korea.
An
Ren Bo
RB
Yoon
Na Young
NY
Nam
Taek Jeong
TJ
Choi
Jae Sue
JS
D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005591:Chemical Fractionation / Q000379:methods; D004146:Dioxanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004147:Dioxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D000459:Phaeophyceae / Q000737:chemistry*; D010696:Phloroglucinol / Q000737:chemistry / Q000494:pharmacology; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000077385:Silybin; D012838:Silymarin; D013619:Tacrine / Q000494:pharmacology; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D001572:Benzofurans; D004146:Dioxanes; D004147:Dioxins; D006576:Heterocyclic Compounds, 4 or More Rings; D020011:Protective Agents; D012838:Silymarin; D013634:Tannins; C507565:eckstolonol; C064093:phlorofucofuroeckol A; D000431:Ethanol; D000077385:Silybin; D013619:Tacrine; D010696:Phloroglucinol