Phylogenetic relationships of Brazilian bovine respiratory syncytial virus isolates and molecular homology modeling of attachment glycoprotein.
Bovine respiratory syncytial virus (BRSV) causes lower respiratory tract disease in young cattle. Recently, it was possible to determine the sequence of the G protein gene, which plays a role in the attachment of BRSV particles to the cells, from three distinct Brazilian isolates. The phylogenetic analysis conducted here using those sequences compared to other worldwide distributed isolates of BRSV allow us to allocate Brazilian strains within the subgroup B, which was no longer found in the world since the 1970s. One of the Brazilian strains has a major mutation between amino acid residues 173 and 178, within the central hydrophobic conserved region, exactly on the site of two of the four cysteine-noose forming cysteine residues. Homology modeling with the previously determined NMR structure of this protein domain was made to check whether these mutations altered the three-dimensional conformation of this immunodominant region. Possible consequences on the biological effects induced by such mutation on the G protein are discussed.
Spilki
Fernando Rosado
FR
Laboratório de Virologia Animal, Instituto de Biologia, UNICAMP, P.O. Box 6109, 13084-970 Campinas, Brazil.
Almeida
Renata Servan
RS
Domingues
Helena Gallichio
HG
D'Arce
Regina Celia Freitas
RC
Ferreira
Helena Lage
HL
Campalans
Jaqueline
J
Costa
Sandra Cecília Botelho
SC
Arns
Clarice Weis
CW
D000595:Amino Acid Sequence; D000818:Animals; D001938:Brazil; D002417:Cattle; D019295:Computational Biology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D018357:Respiratory Syncytial Virus Infections / Q000662:veterinary / Q000821:virology; D018112:Respiratory Syncytial Virus, Bovine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics*
C066028:G protein, Bovine respiratory syncytial virus; D014759:Viral Envelope Proteins