title: [Studies on the changes of 5-hydroxymethyl furfuraldehyde content in Radix Rehmanniae steamed for different time].
abstract: OBJECTIVE
  To find the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Radix Rehmanniae steamed for different time.
  
  
  METHODS
  HPLC method was used, with C18 column (6 mm x 200 mm, 5 microm), a mixture of methanol-water (15: 85) as the mobile phase. The flow rate was 1.0 mL x min(-1). The UV detection was setat 284 nm and the column temperature was set at 24 degrees C.
  
  
  RESULTS
  The linear range was from 0.01 to 0.08 microg, r = 0.999 9 (n = 5). The average recovery was 100.2% and RSD was 2.7% (n = 5).
  
  
  CONCLUSIONS
  The 5-HMF content increases as the processing time prolongs in a certain time rang.
authors.affiliation: Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China. lijun@hactcm.edu.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Zhen-Ling
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D006358:Hot Temperature; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D032267:Rehmannia / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors
chemical_list: C008046:5-hydroxymethylfurfural; D005662:Furaldehyde