title: Effect of antispermatogenic agents on cell marker enzymes of rat Sertoli cells in vitro.
abstract: OBJECTIVE
  To examine the role of Sertoli cells in the antispermatogenic action of two nonsteroidal male contraceptive compounds (CDRI-84/35 and gossypol) by evaluating their effect on some key parameters of Sertoli cell function in vitro.
  
  
  METHODS
  Primary cultures of Sertoli cell were established from 18-day-old rat testis and treated on day 5 with different concentrations (1.0, 0.1, 0.01, and 0.001 mM) of either CDRI-84/35 or gossypol in vitro. Lactate (secretion), along with beta-glucuronidase, gamma-glutamyl transpeptidase, lactate dehydrogenase (LDH) and aromatase activities, was measured in these cells to examine the functions targeted by antispermatogenic agents in Sertoli cells.
  
  
  RESULTS
  CDRI-84/35 significantly affected Sertoli cell parameters (stimulation in most of the cases) that are important for germ cell development like lactate secretion, LDH activity, aromatase activity (estradiol secretion) and so on. Gossypol in comparison to CDRI-84/35 had a more severe effect on Sertoli cells with complete inhibition of enzyme activities at higher concentrations.
  
  
  CONCLUSIONS
  It is probable that the antispermatogenic action of CDRI-84/35 and gossypol is routed through Sertoli cells by disruption of important cell functions that support spermatogenesis in vivo. However, the two compounds appear to have different course of action in Sertoli cells, ultimately leading to spermatogenic failure.
authors.affiliation: Division of Endocrinology, Central Drug Research Institute, Lucknow-226 001, India.
authors.forename: Priti
authors.initials: P
authors.lastname: Ojha
authors.forename: Janak Dulari
authors.initials: JD
authors.lastname: Dhar
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Dwivedi
authors.forename: Ram Lakhan
authors.initials: RL
authors.lastname: Singh
authors.forename: Gopal
authors.initials: G
authors.lastname: Gupta
mesh_terms: D000818:Animals; D000988:Antispermatogenic Agents / Q000494:pharmacology*; D001141:Aromatase / Q000378:metabolism; D002478:Cells, Cultured; D005966:Glucuronidase / Q000378:metabolism; D006072:Gossypol / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000187:drug effects* / Q000201:enzymology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: C111137:1-formyl-4-dichloroacetylpiperazine; D000988:Antispermatogenic Agents; D010879:Piperazines; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D001141:Aromatase; D005723:gamma-Glutamyltransferase; D005966:Glucuronidase; D006072:Gossypol