Dietary isothiocyanates modify mitochondrial functions through their electrophilic reaction.
We found that both benzyl isothiocyanate (ITC) and phenyl ITC inhibited respiration in the mitochondria in an electrophilic reaction-dependent manner. ITC-induced mitochondrial swelling and cytochrome c release were prevented by cyclosporin A, indicating that they are mediated through the ITC moiety-dependent reaction to critical thiol groups for the opening of membrane permeability transition-dependent pores.
Kawakami
Makiko
M
Department of Biochemistry, School of Medicine, Fujita Health University, Toyoake, Japan.
Harada
Nobuhiro
N
Hiratsuka
Mio
M
Kawai
Kiyoshi
K
Nakamura
Yoshimasa
Y
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology; D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism; D004032:Diet; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D017879:Isothiocyanates / Q000494:pharmacology*; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008933:Mitochondrial Swelling / Q000187:drug effects; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010539:Permeability / Q000187:drug effects; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D013859:Thiocarbamates / Q000494:pharmacology; D013861:Thiocyanates / Q000494:pharmacology
D017879:Isothiocyanates; D013859:Thiocarbamates; D013861:Thiocyanates; C005441:phenylisothiocyanate; D016572:Cyclosporine; C031403:benzyl isothiocyanate; D045304:Cytochromes c