title: Evaluation of image processing programs for accurate measurement of budding and fission yeast morphology.
abstract: To study the cellular functions of gene products, various yeast morphological mutants have been investigated. To describe yeast morphology objectively, we have developed image processing programs for budding and fission yeast. The programs, named CalMorph for budding yeast and F-CalMorph for fission yeast, directly process microscopic images and generate quantitative data about yeast cell shape, nuclear shape and location, and actin distribution. Using CalMorph, we can easily and quickly obtain various quantitative data reproducibly. To study the utility and reliability of CalMorph, we evaluated its data in three ways: (1) The programs extracted three-dimensional bud information from two-dimensional digital images with a low error rate (<1%). (2) The absolute values of the diameters of manufactured fluorescent beads calculated with CalMorph were very close to those given in the manufacturer's data sheet. (3) The programs generated reproducible data consistent with that obtained by hand. Based on these results, we determined that CalMorph could monitor yeast morphological changes accompanied by the progression of the cell cycle. We discuss the potential of the CalMorph series as a novel tool for the analysis of yeast cell morphology.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bldg. FSB-101, 5-1-5 Kashiwanoha, 277-8562, Kashiwa, Chiba, Japan.
authors.forename: Genjiro
authors.initials: G
authors.lastname: Suzuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sawai
authors.forename: Miwaka
authors.initials: M
authors.lastname: Ohtani
authors.forename: Satoru
authors.initials: S
authors.lastname: Nogami
authors.forename: Fumi
authors.initials: F
authors.lastname: Sano-Kumagai
authors.forename: Ayaka
authors.initials: A
authors.lastname: Saka
authors.forename: Masashi
authors.initials: M
authors.lastname: Yukawa
authors.forename: Taro L
authors.initials: TL
authors.lastname: Saito
authors.forename: Jun
authors.initials: J
authors.lastname: Sese
authors.forename: Dai
authors.initials: D
authors.lastname: Hirata
authors.forename: Shinichi
authors.initials: S
authors.lastname: Morishita
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ohya
mesh_terms: D002455:Cell Division; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D012984:Software