title: Assessment of a bioactive compound for its potential antiinflammatory property by tight junction permeability.
abstract: Lactobacillus probiotic strains are proving to be abundant sources of bioactive components, including antiinflammatory components. Lifree was made of fruits fermented by Lactobacillus paracasei, Lactobacillus reuterrii and Saccharomyces cerevisiae. This study was designed to test these compounds in cell assays measuring epithelial barrier function and proliferation in the first instance. Cell proliferation was measured in mouse fibroblasts cells (3T3NIH) and rat intestinal epithelial cells (IEC-6), and tight junction activity in the kidney epithelial cell line (MDCK). Tight junction permeability was assessed by measuring transepithelial electrical resistance (TER) across confluent monolayers, following the addition of Lifree with or without a challenge with EGTA. Lifree promoted tight junction formation and recovery following loss of TER from challenge with EGTA. On the other hand, Lifree did not stimulate cell growth in either 3T3NIH and IEC-6 cells. Lifree stimulates tight junction maintenance and formation, suggesting it may have potential antiinflammatory properties.
authors.affiliation: Research Division of Biological Science, Chosun University Medical School, Gwangju, 501-759, Korea.
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Bai
authors.forename: Sok Cheon
authors.initials: SC
authors.lastname: Pak
authors.forename: Seung Hoo
authors.initials: SH
authors.lastname: Lee
authors.forename: Chun Sik
authors.initials: CS
authors.lastname: Bae
authors.forename: Colin
authors.initials: C
authors.lastname: Prosser
authors.forename: Kerst
authors.initials: K
authors.lastname: Stelwagen
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Soon Duck
authors.initials: SD
authors.lastname: Park
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D005285:Fermentation; D005638:Fruit; D007778:Lactobacillus; D051379:Mice; D041681:NIH 3T3 Cells; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D019108:Tight Junctions / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D001688:Biological Products; D010936:Plant Extracts