title: Identification of cutaneous lymphocyte-associated antigen as sialyl 6-sulfo Lewis X, a selectin ligand expressed on a subset of skin-homing helper memory T cells.
abstract: We previously identified the carbohydrate determinant sialyl 6-sulfo Lewis X (Le(x)) as the major L-selectin ligand on high endothelial venules of peripheral lymph nodes. In this study, we examined the distribution of the sialyl 6-sulfo Le(x) determinant among peripheral lymphocytes. The determinant was expressed on a subset of helper memory T and NK cells. The helper memory T cells expressing sialyl 6-sulfo Le(x) were CD45RO(bright+) PSGL-1(high+) CCR4+ L-selectin+ CCR7+ but did not express alpha4beta7 integrin or CCR9, indicating that they were the skin-homing population of central memory T cells. The T-cell subset significantly expressed mRNA for 6-sulfotransferase HEC-GlcNAc6ST and fucosyltransferase Fuc-T VII, responsible for the synthesis of sialyl 6-sulfo Le(x). Characteristics of the T-cell population were similar to those previously described for cutaneous lymphocyte-associated antigen (CLA)-positive T cells defined by the HECA-452 or 2F3 antibody. The binding of the T-cell subset with the specific anti-sialyl 6-sulfo Le(x) antibody G152 was almost completely abrogated by HECA-452 or 2F3. Binding of recombinant E-, P-, and L-selectins to the T-cell subset was significantly inhibited by G152 and by HECA-452 antibodies. We propose that CLA, which is expressed without any activation stimuli on peripheral skin-homing helper memory T cells in healthy persons, is at least partly the sialyl 6-sulfo Le(x) determinant.
authors.affiliation: Department of Clinical Pathology, Kyoto University School of Medicine, Japan.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Ohmori
authors.forename: Fumiyo
authors.initials: F
authors.lastname: Fukui
authors.forename: Makoto
authors.initials: M
authors.lastname: Kiso
authors.forename: Toshio
authors.initials: T
authors.lastname: Imai
authors.forename: Osamu
authors.initials: O
authors.lastname: Yoshie
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Kouji
authors.initials: K
authors.lastname: Matsushima
authors.forename: Reiji
authors.initials: R
authors.lastname: Kannagi
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D005647:Fucosyltransferases / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D039441:Integrin alpha4 / Q000276:immunology; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology; D019041:L-Selectin / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000276:immunology; D008024:Ligands; D008562:Membrane Glycoproteins / Q000276:immunology; D009844:Oligosaccharides / Q000276:immunology*; D054388:Receptors, CCR; D019707:Receptors, Chemokine / Q000276:immunology; D000080506:Sialyl Lewis X Antigen; D012867:Skin / Q000166:cytology / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; C119309:CC chemokine receptor 9; D008024:Ligands; D008562:Membrane Glycoproteins; D009844:Oligosaccharides; C084882:P-selectin ligand protein; D054388:Receptors, CCR; D019707:Receptors, Chemokine; D000080506:Sialyl Lewis X Antigen; D019041:L-Selectin; D039441:Integrin alpha4; D005647:Fucosyltransferases; D017493:Leukocyte Common Antigens