The effects of oxidative stress on outcomes of assisted reproductive techniques.
OBJECTIVE
To investigate the impact of oxidative stress on pregnancy success by monitoring malondialdehyde levels in follicular fluid.


METHODS
Forty five couples were enrolled in this prospective study. Following long protocol of GnRH analogues and r-FSH treatment, oocyte retrieval and intracytoplasmic sperm injection were performed. Malondialdehyde levels were assayed by thiobarbutiric acid reacting substances test. Student's t-test and chi(2) test were used for statistical analysis.


RESULTS
Patients were divided into two groups; group I (pregnancy positive, n = 20), group II (pregnancy negative, n = 25). There was no statistical significant difference in terms of age, infertility period, FSH levels on the third day, number of oocytes retrieved and fertilization rates between the two groups. Pregnancy rates were found to be decreasing in higher malondialdehyde levels.


CONCLUSIONS
Malondialdehyde can be used as a marker of oxidative stress and a potential marker in predicting assisted reproductive techniques outcome.
Oral
Ozay
O
Zeynep Kamil Training and Research Hospital, Assisted Reproductive Techniques Department, Istanbul, Turkey.
Kutlu
Tayfun
T
Aksoy
Evrim
E
Fiçicioğlu
Cem
C
Uslu
Hüsamettin
H
Tuğrul
Semih
S
D000328:Adult; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D027724:Reproductive Techniques, Assisted
D008315:Malondialdehyde