Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies.
The aim of this study was to investigate the expression of osteocalcin (OCN) splicing variants in hematological malignancies. We analysed bone marrow obtained from two patients with chronic myeloid leukemia (CML), seven patients with other myeloproliferative diseases (MPD) and four patients with acute myeloid leukemia (AML). RT-PCR analyses were performed in order to assess and quantify spliced (OCNs) and unspliced (OCNu) mRNA, the associated transcription factors (AML1 and AML3) as well as c-KIT which is a marker for activated stem cells. Our data indicate that OCNs mRNA and OCN protein is expressed in c-KIT positive neoplastic stem cells in hematological malignancies.
Wihlidal
Peter
P
Ludwig Boltzmann Institute for Leukemia Research and Hematology, Hanusch Hospital, Vienna, Austria.
Varga
Franz
F
Pfeilstöcker
Michael
M
Karlic
Heidrun
H
D017398:Alternative Splicing; D001854:Bone Marrow Cells / Q000473:pathology; D017931:DNA Primers; D018450:Disease Progression; D019337:Hematologic Neoplasms / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D009196:Myeloproliferative Disorders / Q000235:genetics; D015675:Osteocalcin / Q000235:genetics*; D014176:Protein Biosynthesis; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000473:pathology; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D015675:Osteocalcin; D019009:Proto-Oncogene Proteins c-kit