Evaluation of column bleed by using an ultraviolet and a charged aerosol detector coupled to a high-temperature liquid chromatographic system.
In this study, five different HPLC columns were heated to 200 degrees C using a homemade heating system which can be operated in temperature programmed mode. The column bleed as an indicator of induced degradation of the stationary phase material was evaluated using a charged aerosol detector (CAD) and an ultraviolet diode array detector (UV-DAD) at different wavelengths. The silica based C-18 stationary phase gave the highest bleed, and the carbon clad titanium dioxide column the lowest bleed. This was independent of both the detection technique and the wavelength.
Teutenberg
T
T
Institute of Energy and Environmental Technology (IUTA), Duisburg, Germany. teutenberg@iuta.de
Tuerk
J
J
Holzhauser
M
M
Kiffmeyer
T K
TK
D000336:Aerosols; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D009862:Online Systems; D014466:Ultraviolet Rays
D000336:Aerosols