Immunolocalization of androgen receptor in the epididymis of rats with dihydrotestosterone deficiency.
Dihydrotestosterone (DHT), 5alpha-reduced metabolite of testosterone, is the most potent androgen in the epididymis. The conversion of T into DHT is carried out by 5alpha-reductase. The activity of 5alpha-reductase type 2, preferentially expressed in the epididymis can be inhibited by a finasteride (a steroid-based specific inhibitor of 5alpha-reductase type 2) which results in DHT deficiency. The aim of the study was to examine the morphology of epididymis and the immunolocalization of an androgen receptor (AR) in the initial segment, caput and cauda epididymis of rats treated with finasteride for 56 days. There were no morphological changes in the morphology of epididymal epithelium in the experimental rats. Immunostainable AR was localized in nuclei of epithelial cells, smooth muscle cells and mainly in the cytoplasm of interstitial cells in the epididymis of control rats. In the epididymis of experimental rats, AR immunostaining was noticed mainly in the cytoplasm of epithelial cells and interstitial cells. The single cells of the initial segment epithelium, basal cells and smooth muscle cells of cauda epididymis showed nuclear AR staining. In conclusion, finasteride affected the expression of the AR in the rat epididymis without changing the morphology of epididymal epithelium. Altered AR expression reflected the hormonal status within the epididymis.
Trybek
Grzegorz
G
Department of Histology and Embryology, Pomeranian Medical University in Szczecin, Szczecin, Poland.
Kolasa
Agnieszka
A
Marchlewicz
Mariola
M
Wenda-Rózewicka
Lidia
L
Wiszniewska
Barbara
B
D058891:5-alpha Reductase Inhibitors; D000818:Animals; D013196:Dihydrotestosterone / Q000378:metabolism*; D004822:Epididymis / Q000166:cytology / Q000378:metabolism*; D018120:Finasteride / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000378:metabolism*
D058891:5-alpha Reductase Inhibitors; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D018120:Finasteride