[Analysis of the factors which influence allograft calcification after aorta transplantation of allogenetic rat].
OBJECTIVE
To explore the factors which influence the calcification of homograft after aorta transplantation of allogenetic rat.


METHODS
The research was devided into 2 groups: allogene group and isogenenic group. Allogene group: SD -->Wistar. Isogenenic group: Wistar to Wistar. Aortic valve homograft was heterotopically allografted onto abdominal aorta. The rats were sacrificed in batches at 2, 4, 8, 12 and 16 weeks postoperatively. Blood samples were obtained for accessing the expression of CD25, CD71, and AVH was obtained for accessing the calcium level and the expression of CD40. At the same time, the change of endotheliocyte and smooth muscle cells were observed with transmission electron microscope.


RESULTS
(1)Compared with the isogene group, the expression of CD40, CD25 and CD71 in allogene group was much higher at each time point and reached peak at 2-4 weeks after operation. (2)The calcium level in allogene group increased at 4 weeks after operation and reached the peak at 12 weeks after operation. No significant difference in calcium level was found in isogenenic group over 5 different periods. (3)Exfoliation of endotheliacytes as well as necrosis of smooth muscle cells were observed in the graft in allogene group.


CONCLUSIONS
The calcium level of homograft had a relation with immunological rejection. The calcification began at 4 weeks postoperation; the calcium level of homogaft increased gradually and reached the peak at 12 weeks postoperation and then maintained on a stable level.
Chang
Qing
Q
Department of Cardiovascular Surgery, Qilu Hospital, Shandong University, Jinan 250012, China. changqing20671@163.com
Li
Shou-xian
SX
Xu
Ping
P
Lu
Zhen-hua
ZH
Gong
Xing-jun
XJ
Liu
Xiang-ping
XP
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001011:Aorta / Q000637:transplantation* / Q000648:ultrastructure; D019013:CD40 Antigens / Q000378:metabolism; D002114:Calcinosis / Q000097:blood / Q000276:immunology*; D002118:Calcium / Q000097:blood; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D011990:Receptors, Transferrin / Q000378:metabolism; D014184:Transplantation, Homologous / Q000276:immunology*; D014185:Transplantation, Isogeneic / Q000276:immunology*
D015703:Antigens, CD; D019013:CD40 Antigens; C068126:CD71 antigen; D053645:Interleukin-2 Receptor alpha Subunit; D011990:Receptors, Transferrin; D002118:Calcium