title: Characterization of a novel PepF-like oligopeptidase secreted by Bacillus amyloliquefaciens 23-7A.
abstract: An oligopeptidase from Bacillus amyloliquefaciens 23-7A was characterized along with its biochemical activities and structural gene. The protein's amino acid sequence and enzymatic activities were similar to those of other bacterial PepFs, which belong to metallopeptidase family M3. While most bacterial PepFs are cytoplasmic endopeptidases, the identified PepFBa oligopeptidase is a secreted protein and may facilitate the process of sporulation.
authors.affiliation: Institute of Biochemistry, National Yang-Ming University, 155, Sec. 2, Li-Nong Street, Pei-Tou, Taipei 11221, Taiwan.
authors.forename: Shiou-Huei
authors.initials: SH
authors.lastname: Chao
authors.forename: Tzu-Hao
authors.initials: TH
authors.lastname: Cheng
authors.forename: Chin-Ying
authors.initials: CY
authors.lastname: Shaw
authors.forename: Meng-Hwan
authors.initials: MH
authors.lastname: Lee
authors.forename: Yuan-Hsun
authors.initials: YH
authors.lastname: Hsu
authors.forename: Ying-Chieh
authors.initials: YC
authors.lastname: Tsai
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008666:Metalloendopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D008666:Metalloendopeptidases; C091036:Oligoendopeptidase F