Plectin rodless isoform expression and its detection in mouse brain.
The widely expressed cytolinker protein plectin shows extensive isoform diversity both at the N-terminus and in the central part of the molecule. Judged on mRNA data, plectin variants lacking the central rod domain are expressed at a approximately 20-fold lower level than full-length proteins and their detection on the protein level can be difficult. Here we present data on the expression of plectin rodless isoforms in mouse brain and in rat glioma C6 cells on RNA and protein levels. Our data indicate that among the rodless variants expressed in neuronal tissues, those starting with exon 1c (plectin 1c) seem to be the most prominent ones. In addition, we show that similar to other monoclonal antibodies reported in the literature, the widely used mAb 7A8 recognizes an epitope within plectin's rod domain and therefore is unsuited to detect rodless variants of plectin.
Fuchs
Peter
P
Department of Molecular Cell Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
Spazierer
Daniel
D
Wiche
Gerhard
G
D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D001681:Biological Assay; D001921:Brain / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D005910:Glioma / Q000235:genetics; D051379:Mice; D051190:Plectin / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism
D018076:DNA, Complementary; D051190:Plectin; D020033:Protein Isoforms; D012333:RNA, Messenger; D012260:Ribonucleases