title: T-cell recognition of a prostate specific antigen is not sufficient to induce prostate tissue destruction.
abstract: METHODS
  The ability of CD8(+) T-cells to induce prostate inflammation was examined using a prostate ovalbumin expressing transgenic mouse (POET) and/or adoptive transfer of T-cell receptor (TCR) transgenic T-cells (OT-I) that specifically recognize ovalbumin. Localization of inflammatory cells to prostate tissue was examined following T-cell activation via endogenous prostatic antigen, recombinant type 5 adenovirus carrying the gene coding ovalbumin (Ad5-mOVA), or adoptive transfer of in vitro antigen stimulated OT-I cells.
  
  
  RESULTS
  Ovalbumin specific OT-I cells were activated by autologous prostate antigen and trafficked to the prostate, but did not induce inflammation unless present in overwhelming numbers ( approximately 65% of CD8(+) T-cells). Activation of antigen specific CD8(+) T-cells in vitro (peptide pulsed antigen presenting cells) or in vivo (Ad5-mOVA) induced transitory prostate inflammation, without induction of prostate pathology, regardless of CD4(+) T-cell availability. Inflammation also was observed in OT-I x POET mice but again, pathological effects were not observed.
  
  
  CONCLUSIONS
  T lymphocytes specific for a prostate antigen are capable of inducing inflammatory infiltration of prostatic tissue rapidly following activation, but do not produce pathological prostate injury.
authors.affiliation: Interdisciplinary Program in Immunology, University of Iowa, Iowa City, Iowa 52242, USA.
authors.forename: Jason R
authors.initials: JR
authors.lastname: Lees
authors.forename: Bridget
authors.initials: B
authors.lastname: Charbonneau
authors.forename: John D
authors.initials: JD
authors.lastname: Hayball
authors.forename: Kerrilyn
authors.initials: K
authors.lastname: Diener
authors.forename: Michael
authors.initials: M
authors.lastname: Brown
authors.forename: Robert
authors.initials: R
authors.lastname: Matusik
authors.forename: Michael B
authors.initials: MB
authors.lastname: Cohen
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Ratliff
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D017931:DNA Primers; D007150:Immunohistochemistry; D007249:Inflammation; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000235:genetics*; D011467:Prostate / Q000276:immunology / Q000473:pathology*; D017430:Prostate-Specific Antigen / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013950:Thymus Gland / Q000276:immunology; D001743:Urinary Bladder / Q000276:immunology / Q000473:pathology
chemical_list: D016207:Cytokines; D017931:DNA Primers; D010047:Ovalbumin; D017430:Prostate-Specific Antigen