title: Extended lifespan and long telomeres in rectal fibroblasts from late-onset ulcerative colitis patients.
abstract: OBJECTIVE
  Ulcerative colitis (UC) is characterized by damage to the intestinal epithelium and connective tissue. The causes of this damage could include changes in the ability of colonic fibroblasts to heal wounds and maintain epithelial cell proliferation. Telomeres shorten with each cell division and eventually signal senescence. The aim of this study is to investigate whether the impaired function of rectal fibroblasts in UC is due to accelerated telomere shortening, oxidative stress and premature senescence.
  
  
  METHODS
  We isolated rectal fibroblasts from eight UC patients and nine non-colitis controls, and recorded their in-vitro lifespans. Telomere lengths and superoxide dismutase mRNA expression were also measured by real-time polymerase chain reaction and peroxide levels were measured by flow cytometry.
  
  
  RESULTS
  The fibroblast lifespan decreased as patient age increased (R2=0.68, P=0.003) in control patients, but this relationship was absent in UC fibroblasts. We identified a group of patients who were diagnosed later in life than a second group (59 versus 35 years, P=0.002). Fibroblasts from these late-onset UC patients underwent significantly more population doublings before senescence than age-matched controls (25 versus 15, P=0.02). Slower in-vitro telomere shortening rates (32 versus 344, P=0.006) and trends towards longer telomeres at explant were also observed in late-onset UC fibroblasts. Peroxide levels correlated positively with telomere shortening rate (r=0.581, P=0.078).
  
  
  CONCLUSIONS
  Some UC-predisposed individuals may have more efficient antioxidant systems that protect the telomeres from oxidative damage. This may allow their rectal fibroblasts to live longer, function better and thus delay the onset of the disease until later life.
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry, UK.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Getliffe
authors.forename: Carmen
authors.initials: C
authors.lastname: Martin Ruiz
authors.forename: João F
authors.initials: JF
authors.lastname: Passos
authors.forename: Thomas
authors.initials: T
authors.lastname: von Zglinicki
authors.forename: Chuka U
authors.initials: CU
authors.lastname: Nwokolo
mesh_terms: D000328:Adult; D017668:Age of Onset; D000368:Aged; D002470:Cell Survival; D002478:Cells, Cultured; D003093:Colitis, Ulcerative / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012007:Rectum / Q000201:enzymology / Q000473:pathology*; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics; D019098:Telomerase / Q000096:biosynthesis / Q000235:genetics; D016615:Telomere / Q000473:pathology*
chemical_list: D004268:DNA-Binding Proteins; D012333:RNA, Messenger; C422952:telomerase RNA; D012313:RNA; C441642:SOD3 protein, human; D013482:Superoxide Dismutase; D019098:Telomerase