title: Functional expression of transient receptor potential melastatin- and vanilloid-related channels in pulmonary arterial and aortic smooth muscle.
abstract: Transient receptor potential melastatin- (TRPM) and vanilloid-related (TRPV) channels are nonselective cation channels pertinent to diverse physiological functions. Multiple TRPM and TRPV channel subtypes have been identified and cloned in different tissues. However, their information in vascular tissue is scant. In this study, we sought to identify TRPM and TRPV channel subtypes expressed in rat deendothelialized intralobar pulmonary arteries (PAs) and aorta. With RT-PCR, mRNA of TRPM2, TRPM3, TRPM4, TRPM7, and TRPM8 of TRPM family and TRPV1, TRPV2, TRPV3, and TRPV4 of TRPV family were detected in both PAs and aorta. Quantitative real-time RT-PCR showed that TRPM8 and TRPV4 were the most abundantly expressed TRPM and TRPV subtypes, respectively. Moreover, Western blot analysis verified expression of TRPM2, TRPM8, TRPV1, and TRPV4 proteins in both types of vascular tissue. To examine the functional activities of these channels, we monitored intracellular Ca(2+) transients ([Ca(2+)](i)) in pulmonary arterial smooth muscle cells (PASMCs) and aortic smooth muscle cells (ASMCs). The TRPM8 agonist menthol (300 muM) and the TRPV4 agonist 4alpha-phorbol 12,13-didecanoate (1 muM) evoked significant increases in [Ca(2+)](i) in PASMCs and ASMCs. These Ca(2+) responses were abolished in the absence of extracellular Ca(2+) or the presence of 300 muM Ni(2+) but were unaffected by 1 muM nifedipine, suggesting Ca(2+) influx via nonselective cation channels. Hence, for the first time, our results indicate that multiple functional TRPM and TRPV channels are coexpressed in rat intralobar PAs and aorta. These novel Ca(2+) entry pathways may play important roles in the regulation of pulmonary and systemic circulation.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA.
authors.forename: Xiao-Ru
authors.initials: XR
authors.lastname: Yang
authors.forename: Mo-Jun
authors.initials: MJ
authors.lastname: Lin
authors.forename: Lionel S
authors.initials: LS
authors.lastname: McIntosh
authors.forename: James S K
authors.initials: JS
authors.lastname: Sham
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic; D001483:Base Sequence; D020013:Calcium Signaling / Q000502:physiology; D017931:DNA Primers; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D011651:Pulmonary Artery; D051381:Rats; D017208:Rats, Wistar; D050053:TRPM Cation Channels / Q000235:genetics*; D050916:TRPV Cation Channels / Q000235:genetics*
chemical_list: D017931:DNA Primers; D050053:TRPM Cation Channels; D050916:TRPV Cation Channels; C505767:TRPV3 protein, rat; C493922:Trpv1 protein, rat; C476722:Trpv2 protein, rat; C486577:Trpv4 protein, rat