title: Cryopreservation of canine ovaries by vitrification.
abstract: The cryopreservation of ovarian tissues is a technology with significant potential for the preservation of the genetic resource materials of working dogs, including guide dogs for the blind. However, no attempt has been reported on cryopreservation of the canine ovary. Thus, we evaluated a vitrification method for cryopreservation of canine ovaries and determined the potential functionality of vitrified-warmed canine ovaries by means of transplantation into non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mice. All ovarian tissues cryopreserved by vitrification were morphologically normal in terms of histology. Cryopreserved ovaries were transplanted into the ovarian bursa of the NOD-SCID mice, and the xenografts were recovered from 23 of 23 mice (100%) 4 weeks after the operation. The transplanted canine tissue was tightly adhered to the mouse ovary. Although antral follicle formation did not occur after grafting, proliferating cell nuclear antigen immunoreactivity was detectable in many of the granulosa cells in the primary follicles of the grafts. These results indicate that cryopreservation of the canine ovary by vitrification appears to have the potential to restore endocrine function and ovulation potential.
authors.affiliation: Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Ishijima
authors.forename: Yoshiyasu
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Dong-Soo
authors.initials: DS
authors.lastname: Lee
authors.forename: Yoshiko Yanagimoto
authors.initials: YY
authors.lastname: Ueta
authors.forename: Motozumi
authors.initials: M
authors.lastname: Matsui
authors.forename: Jung-Youn
authors.initials: JY
authors.lastname: Lee
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Suwa
authors.forename: Kazuro
authors.initials: K
authors.lastname: Miyahara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D004285:Dogs; D004624:Embryo Transfer; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D006080:Ovarian Follicle / Q000473:pathology; D010053:Ovary / Q000378:metabolism* / Q000473:pathology; D014021:Tissue Preservation
chemical_list: D003451:Cryoprotective Agents; D018076:DNA, Complementary