Multilocus sequence typing of Porphyromonas gingivalis strains from different geographic origins.
Porphyromonas gingivalis is an important periodontal pathogen that can be isolated from both active and inactive periodontal lesions. Apparently, differences in virulence between P. gingivalis strains exist, but the mechanisms underlying these differences are not yet fully understood. To obtain more information about pathogenicity and virulence of P. gingivalis, it is relevant to assess the genetic population structure of the species and to examine the occurrence of putative virulence factors against the genetic background. Presently, multilocus sequence typing (MLST) is the best method for analyzing bacterial population structures. Forty P. gingivalis strains from worldwide sources were analyzed by MLST. Internal 310- to 420-bp DNA fragments of the eight ubiquitous chromosomal genes, ftsQ, hagB, gdpxJ, pepO, mcmA, recA, pga, and nah, were amplified by PCR and then sequenced. The number of alleles at individual loci ranged from 2 to 19, and a total of 33 allelic profiles, or sequence types (STs), were identified. Nucleotide variation between alleles was located at one or a few sites. Identical or similar STs were found in isolates from different geographic regions. Our results showed signs of a clonal population structure with a level of recombination not as high as that previously suggested for the species. We also found that P. gingivalis isolates from individual patients were genetically heterogeneous.
Enersen
Morten
M
Institute of Oral Biology, Dental Faculty, University of Oslo, Norway. morteene@odont.uio.no
Olsen
Ingar
I
van Winkelhoff
Arie J
AJ
Caugant
Dominique A
DA
D000483:Alleles; D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D006801:Humans; D010802:Phylogeny; D016966:Porphyromonas gingivalis / Q000145:classification* / Q000235:genetics; D011995:Recombination, Genetic
D004269:DNA, Bacterial