title: [Effects of bushen huoxue recipe on proliferation, differentiation and mineralization of osteoblasts in rats].
abstract: OBJECTIVE
  To observe the effects of bushen huoxue recipe (BHR) on cell proliferation, differentiation, maturation and mineralization in osteoblasts of rats cultured in vitro.
  
  
  METHODS
  Osteoblasts from cranium of newborn SD rats were cultured by collagenase method. MTT, PNPP and ARS were used to observe the proliferation, activity of alkaline phosphatase (ALP) and the formation of mineral node of cultured osteoblasts effected by different concentrations of BHR at different time points (24, 48, 72 hrs after medication).
  
  
  RESULTS
  Different concentrations of BHR could enhance the cell proliferation rate and ALP activity, the effects of BHR in high and moderate concentration were more significant in enhancing the cell proliferation rate (P < 0.01). And the number of mineral node of cultured osteoblasts treated by high and moderate concentration BHR was much more than that of the untreated osteoblasts (P < 0.01).
  
  
  CONCLUSIONS
  BHR could promote the proliferation, differentiation, and mineralization of the osteoblasts.
authors.affiliation: Department of Orthopedics, Second Hospital of Xi' an Jiaotong University, Xi' an 710044. zhm197@163.com
authors.forename: Hong-min
authors.initials: HM
authors.lastname: Zhang
authors.forename: Jun-chang
authors.initials: JC
authors.lastname: Chen
authors.forename: Yi-heng
authors.initials: YH
authors.lastname: Liu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002113:Calcification, Physiologic / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D000469:Alkaline Phosphatase; D002118:Calcium