Cytotoxicity, haematotoxicity and genotoxicity of high molecular mass arborescent polyoxyethylene polymers with polyglycidol-block-containing shells.
We have examined the impact on biological systems of some newly synthesised polyoxyethylene polymers using in vitro assays. Toxicity was tested by the 3-[4,5-dimethylthiazole-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay, haemolysis was assessed, and an ethidium bromide (EB) assay was used to study interactions between the polymers and DNA. All the assay data showed that the polymers are biocompatible. No differences were found between generations (i.e. macromolecule sizes). The results encourage continuing studies on the clinical use of these molecules as drug carriers.
Klajnert
Barbara
B
Department of General Biophysics, University of Lodz, 12/16 Banacha St., 90-237 Lodz, Poland.
Walach
Wojciech
W
Bryszewska
Maria
M
Dworak
Andrzej
A
Shcharbin
Dzmitry
D
D000818:Animals; D016466:CHO Cells; D016923:Cell Death / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004996:Ethidium; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008970:Molecular Weight; D009153:Mutagens / Q000633:toxicity*; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000633:toxicity*; D011108:Polymers / Q000737:chemistry / Q000633:toxicity*; D011409:Propylene Glycols / Q000737:chemistry* / Q000633:toxicity; D017186:Titrimetry
D009153:Mutagens; D011108:Polymers; D011409:Propylene Glycols; C502907:polyglycidol; D011092:Polyethylene Glycols; D004996:Ethidium