The REFOLD database: a tool for the optimization of protein expression and refolding.
A large proportion of proteins expressed in Escherichia coli form inclusion bodies and thus require renaturation to attain a functional conformation for analysis. In this process, identifying and optimizing the refolding conditions and methodology is often rate limiting. In order to address this problem, we have developed REFOLD, a web-accessible relational database containing the published methods employed in the refolding of recombinant proteins. Currently, REFOLD contains >300 entries, which are heavily annotated such that the database can be searched via multiple parameters. We anticipate that REFOLD will continue to grow and eventually become a powerful tool for the optimization of protein renaturation. REFOLD is freely available at http://refold.med.monash.edu.au.
Chow
Michelle K M
MK
Department of Biochemistry and Molecular Biology, PO Box 13D, Monash University, Victoria 3800, Australia.
Amin
Abdullah A
AA
Fulton
Kate F
KF
Fernando
Thushan
T
Kamau
Lawrence
L
Batty
Chris
C
Louca
Michael
M
Ho
Storm
S
Whisstock
James C
JC
Bottomley
Stephen P
SP
Buckle
Ashley M
AM
D030562:Databases, Protein; D015870:Gene Expression; D020407:Internet; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism*; D014584:User-Computer Interface
D011994:Recombinant Proteins