Recognition of chromosomal DNA in human cells by peptide nucleic acids and small duplex RNAs.
Inhibition of translation by duplex RNA (siRNA) complementary to mRNA is a powerful approach to silencing genes in mammalian cells and RNA interference (RNAi) is an important natural biological mechanism for controlling gene expression. Anti-mRNA duplexes are widely used for laboratory studies, target validation, and therapeutic development. Endogenously expressed duplex RNAs (microRNAs, miRNAs) have been shown to target mRNA and be natural regulators of expression. Recently, we have shown that peptide nucleic acids (PNAs) or duplex antigene RNAs (agRNAs) that target DNA sequences can also inhibit gene transcription. These findings extend gene silencing to targets within chromosomal DNA.
Corey
David R
DR
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9041, USA. david.corey@utsouthwestern.edu
D017398:Alternative Splicing; D004247:DNA / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature; D014158:Transcription, Genetic
D020135:Peptide Nucleic Acids; D012333:RNA, Messenger; D012313:RNA; D004247:DNA