Modulation of costimulatory molecules CD80/CD86 on B cells and macrophages by stress proteins GroEL, GroES and DnaK.
The aim of this study was to evaluate the effect of the Heat Shock Proteins GroES, GroEL and DnaK on the expression of the costimulatory molecules CD80/CD86 in B cells and macrophages. The interactions among these molecules are able to highly influence the immune response through the regulation of cytokine liberation which, on their own, are able to regulate the immunological response by a feedback mechanism. Our results showed that, on B cells, GroES and GroEL stimulated the expression of CD86 but did not induce the increase of the CD80 expression. CD86 peak expression showed a peak after 24-48 h of culture and decreased 60h after the stimulation. GroES and GroEL also stimulated the expression of CD80 and CD86 on macrophages. The same HSPs did not modify the expression of CD80 and CD86 on cells having characteristics of activated macrophages, the A-THP-1 cell line. DnaK did not induce any increase in the expression of CD80 and CD86 on lymphocytes or macrophages.
Galdiero
M
M
Dipartimento di Medicina Sperimentale, Sezione di Microbiologia e Microbiologia Clinica, Seconda Universitá degli Studi di Napoli, Italy. marilena.galdiero@unina2.it
Pisciotta
M G
MG
Gorga
F
F
Petrillo
G
G
Marinelli
A
A
Galdiero
E
E
D000328:Adult; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D018122:B7-1 Antigen / Q000096:biosynthesis*; D051940:B7-2 Antigen / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000494:pharmacology*; D018835:Chaperonin 10 / Q000494:pharmacology*; D018834:Chaperonin 60 / Q000494:pharmacology*; D005434:Flow Cytometry; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology*; D006801:Humans; D007155:Immunologic Factors; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
D018122:B7-1 Antigen; D051940:B7-2 Antigen; D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D007155:Immunologic Factors; D007202:Indicators and Reagents