title: Cell-type-specific pathways of neurotensin endocytosis.
abstract: The neurotensin receptor subtype 1 (NTS1) is a G-protein-coupled receptor (GPCR) mediating a large number of central and peripheral effects of neurotensin. Upon stimulation, NTS1 is rapidly internalized and targeted to lysosomes. This process depends on the interaction of the phosphorylated receptor with beta-arrestin. Little is known about other accessory endocytic proteins potentially involved. Here, we investigated the involvement of dynamin, amphiphysin, and intersectin in the internalization of NTS1 receptor-ligand complexes in transfected COS-7 and HEK 293 cells, by using the transferrin receptor as an internal control for the constitutive endocytic pathway. We found that NTS1 endocytosis was not only arrestin-dependent, but also dynamin-dependent in both COS-7 and HEK 293 cells, whereas internalization of the transferrin receptor was independent of arrestin but required dynamin. Overexpression of the SH3 domain of amphiphysin II had no effect on receptor internalization in either cell type. By contrast, overexpression of full-length intersectin or of its SH3 domain (but not of its EH domain) inhibited NTS1 internalization in COS-7 but not in HEK 293 cells. This difference between COS-7 and HEK 293 cells was not attributable to differences in endogenous intersectin levels between the two cell lines. Indeed, the same constructs inhibited transferrin endocytosis equally well in COS-7 and HEK 293 cells. However, immunogold electron microscopy revealed that internalized NTS1 receptors were associated with clathrin-coated pits in COS-7 cells but with smooth vesicles in HEK 293 cells, suggesting that NTS1 internalization proceeds via different endocytic pathways in these two cell types.
authors.affiliation: Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montréal, Québec, Canada.
authors.forename: Cheryl
authors.initials: C
authors.lastname: Savdie
authors.forename: Stephen S G
authors.initials: SS
authors.lastname: Ferguson
authors.forename: J- P
authors.initials: J
authors.lastname: Vincent
authors.forename: Alain
authors.initials: A
authors.lastname: Beaudet
authors.forename: Thomas
authors.initials: T
authors.lastname: Stroh
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D000818:Animals; D019390:Arrestins / Q000235:genetics / Q000378:metabolism; D019556:COS Cells; D002522:Chlorocebus aethiops; D034281:Dynamins / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009496:Neurotensin / Q000378:metabolism*; D009928:Organ Specificity; D021381:Protein Transport; D018028:Receptors, Neurotensin / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection; D000071557:beta-Arrestins
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D019390:Arrestins; D009419:Nerve Tissue Proteins; D018028:Receptors, Neurotensin; D000071557:beta-Arrestins; C115919:intersectin 1; C114036:neurotensin type 1 receptor; D049452:Green Fluorescent Proteins; C075637:amphiphysin; D009496:Neurotensin; D034281:Dynamins