Assigning the phenotype of a natural regulatory T-cell to the human T-cell line, KARPAS-299.
The human CD30-positive anaplastic large (T-) cell lymphoma cell line, KARPAS-299 (DSM ACC31), was established from blast cells in the peripheral blood from a case of non-Hodgkin lymphoma in 1988. We describe the mRNA and surface expression in KARPAS-299 cells of a panel of markers highly restricted to human natural regulatory T-cells and associated with their suppressive activity, including FOXP3, CD25, IL-10, TGF-beta1, CD62L, and Lag-3. Results obtained from co-culturing human peripheral blood leukocytes with KARPAS-299 cells assigned a suppressive phenotype to the latter ones. In conclusion, KARPAS-299 cells show characteristics typical of natural regulatory T-cells and, thus, represent a valuable model for studying regulatory T-cell function, which may also facilitate drug development aimed at the modulation of regulatory T-cell activity for the pharmacological therapy of, for example, autoimmune diseases.
Wolke
Carmen
C
Institute of Experimental Internal Medicine, Otto-von-Guericke University, Magdeburg, Germany.
Tadje
Janine
J
Bukowska
Alicja
A
Täger
Michael
M
Bank
Ute
U
Ittenson
Annelore
A
Ansorge
Siegfried
S
Lendeckel
Uwe
U
D045744:Cell Line, Tumor; D018920:Coculture Techniques; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D053773:Transforming Growth Factor beta1
C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D012333:RNA, Messenger; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1