An endophytic fungus from Nothapodytes foetida that produces camptothecin.
A fungal endophytic isolate, camptothecin, has been isolated from the inner bark of the plant Nothapodytes foetidafrom the Western coast of India. The fungus, which belongs to the family Phycomycetes, produced the anticancer drug lead compound camptothecin (1) when grown in a synthetic liquid medium (Sabouraud broth) under shake flask and bench scale fermentation conditions. Compound 1 was identified by means of chromatographic and spectroscopic methods. It was also compared with an authentic example for its biological activity against a number of human cancer cell lines. Isolation of an organism producing 1 and its fermentation may, in the future, provide an easily accessible source for the production of this anticancer drug precursor molecule.
Puri
Satish Chander
SC
Regional Research Laboratory (CSIR), Canal Road, Jammu Tawi 180001, India.
Verma
Vijeshwar
V
Amna
Touseef
T
Qazi
Ghulam Nabi
GN
Spiteller
Michael
M
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002166:Camptothecin / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004271:DNA, Fungal / Q000302:isolation & purification; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007194:India; D015394:Molecular Structure; D010800:Phycomyces / Q000737:chemistry*; D024301:Plant Bark
D000970:Antineoplastic Agents; D004271:DNA, Fungal; D002166:Camptothecin