title: Fabrication of biological nanostructures by scanning near-field photolithography of chloromethylphenylsiloxane monolayers.
abstract: We demonstrate the fabrication of sub-100-nm DNA surface patterns by scanning near-field optical lithography using a near-field scanning optical microscope coupled to a UV laser and a chloromethylphenylsiloxane (CMPS) self-assembled monolayer (SAM). The process involves 244-nm exposure of the CMPS SAM to create nanoscale patterns of surface carboxylic acid functional groups, followed by their conversion to the N-hydroxysuccinimidyl ester and reaction of the active ester with DNA to spatially control DNA grafting with high selectivity.
authors.affiliation: Department of Chemistry, University of Sheffield, UK.
authors.forename: Shuqing
authors.initials: S
authors.lastname: Sun
authors.forename: Matthew
authors.initials: M
authors.lastname: Montague
authors.forename: Kevin
authors.initials: K
authors.lastname: Critchley
authors.forename: Mu-San
authors.initials: MS
authors.lastname: Chen
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Dressick
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Evans
authors.forename: Graham J
authors.initials: GJ
authors.lastname: Leggett
mesh_terms: D000818:Animals; D002264:Carboxylic Acids / Q000737:chemistry; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D007834:Lasers; D018625:Microscopy, Atomic Force; D049329:Nanostructures; D012833:Siloxanes / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry; D013499:Surface Properties
chemical_list: D002264:Carboxylic Acids; D012833:Siloxanes; D013388:Succinimides; D004247:DNA; C427203:calf thymus DNA