title: Anaplasma marginale: lack of cross-protection between strains that share MSP1a variable region and MSP4.
abstract: In Mexico, there are no commercial alternatives for the immunoprophylaxis of bovine Anaplasmosis, a disease responsible for great economic losses. Blood derived Anaplasma marginale used for immunizing susceptible cattle has shown promising results for homologous protection and controversial results against unrelated strains. The present study examined, under controlled conditions, the cross-protective potential of an immunogen composed of blood derived A. marginale of three strains against challenge with strains not included in the immunogens. Groups 1 and 2 were immunized with blood derived Anaplasma from strains Mexico, Morelos and Yucatan, group 4 with strains Morelos, Veracruz and Yucatan, two more groups (2 and 5) of equal conditions were inoculated with an adjuvant alone. Groups 1, 4 and 5 were challenged with Mexico strain; groups 2 and 3 were challenge-inoculated with strain Veracruz; groups 3 and 5 with strains Veracruz and Mexico as controls. Only animals in group 1, immunized and challenged with strain Mexico showed adequate protection. Both groups challenged with strains not included in the immunogens developed poor protection, while all the controls had to be treated to prevent death.
authors.affiliation: Facultad de Ciencias Biológicas, Universidad Autónoma del Estado de Morelos, Morelos, Mexico.
authors.forename: Verónica
authors.initials: V
authors.lastname: Ocampo Espinoza
authors.forename: J Eduardo Salazar
authors.initials: JE
authors.lastname: Vázquez
authors.forename: Marina Durán
authors.initials: MD
authors.lastname: Aguilar
authors.forename: Miguel Angel García
authors.initials: MA
authors.lastname: Ortiz
authors.forename: Germinal J Cantó
authors.initials: GJ
authors.lastname: Alarcón
authors.forename: Sergio D
authors.initials: SD
authors.lastname: Rodríguez
mesh_terms: D042322:Anaplasma marginale / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D000712:Anaplasmosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D004912:Erythrocytes / Q000382:microbiology; D005260:Female; D008565:Membrane Proteins / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D008565:Membrane Proteins; C085498:Msp4 protein, Anaplasma marginale; C449089:major surface protein 1a, Anaplasma