Single-molecule detection and probe strategies for rapid and ultrasensitive genomic detection.
This paper reviews the current state-of-the-art development of single-molecule detection (SMD)-based methods for ultrasensitive and specific analysis of genomic sequences. We first discuss several newly devised single fluorescent probe strategies that allow separation-free detection of low-abundance DNA sequences, such as quantum dot (QD)-mediated fluorescence resonance energy transfer (FRET) technology and dual-color fluorescence coincidence and colocalization analysis. Various schemes toward single DNA sizing and sequencing in solutions or on surfaces are also reviewed. In the end, we summarize the different microfluidic approaches developed for use with SMD to facilitate rapid, low-volume and quantitative analysis, such as electrokinetic and hydrodynamic single-molecule manipulation techniques.
Yeh
Hsin-Chih
HC
Department of Mechanical Engineering and Whitaker Biomedical Engineering Institute, The Johns Hopkins University, Baltimore, Maryland 21218, USA.
Chao
Shu-Yi
SY
Ho
Yi-Ping
YP
Wang
Tza-Huei
TH
D015342:DNA Probes / Q000235:genetics*; D031541:Fluorescence Resonance Energy Transfer; D008956:Models, Chemical; D045663:Quantum Dots; D015347:RNA Probes / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013050:Spectrometry, Fluorescence
D015342:DNA Probes; D015347:RNA Probes