[Study on anti-cancer components of Fig residues with supper critical fluid CO2 extracting technique].
OBJECTIVE
To optimize Supper Critical CO2 extracting technical (SFE-CO2) methods for extraction of anti-cancer active components of Fig Residues and to investigate the anti-cancer effect of the extract in vitro and in vivo.


METHODS
The anti-cancer activity of extracted compound was measured on U937,95D and AGS cancer cells in vitro by MTT method. The anti-cancer effect of the extraction of Fig Residues was studied on mice transplant liver cancer in vivo.


RESULTS
The SFE-CO2 condition for extraction of the anti-cancer components of Fig Residues was optimized as follows: granularity was 100, the extraction pressure was 30 MPa, the extraction temperature was 45 degrees C, the extraction time was 6 h and the CO2 flux was 12 L x h(-1); The IC50 of anti-cancer active components of Fig Residues on U937, 95D and AGS cells were 70.125 microg x mL(-1), 127.957 microg x mL(-1), 116.000 microg x mL(-1); The anti-cancer active components of Fig Residues inhibited 49.3% of the transplanted liver cancer in the mice.


CONCLUSIONS
The method for extracting the anticancer active components of Fig Residues is stable and reasonable, and the extract from Fig Residues is of the anticancer effect.
Wang
Zhen-Bin
ZB
Jiangsu University, School of Biological and Environmental Engineering, Zhenjiang 212013, China.
Ma
Hai-Le
HL
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D030681:Ficus / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D010946:Plants, Medicinal / Q000737:chemistry; D013274:Stomach Neoplasms / Q000473:pathology; D020298:U937 Cells / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal