Sporadic methicillin resistance in community acquired Staphylococcus aureus in Mozambique.
This study reports the drug resistance and clonal relationship of 24 Staphylococcus aureus community acquired isolates from patients attending Maputo Central Hospital, Mozambique, during one year (2002-2003). All the isolates produced beta-lactamase, six strains were resistant to tetracycline alone, three were resistant to erythromycin alone and one was resistant to trimethoprim-sulfamethoxazole; 11 were susceptible to all other drugs tested. Only one strain showed a multiple resistance pattern, including methicillin resistance. To investigate the clonal relationships we applied the ERIC AP-PCR and the SmaI PFGE RFLP methods. Overlapping drug resistances with these two molecular profiles, no significant correlation was obtained. The emergence of methicillin resistance in a multiple resistant strain is of great concern for resistance spreading surveillance in Mozambique.
Ceccarelli
Daniela
D
Dipartimento di Biologia Cellulare e Sviluppo, Università di Roma, La Sapienza, Italy.
Mondlane
José
J
Sale
Marco
M
Salvia
Anna M
AM
Folgosa
Elena
E
Cappuccinelli
Piero
P
Colombo
Mauro M
MM
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017714:Community-Acquired Infections / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D024901:Drug Resistance, Multiple, Bacterial; D004917:Erythromycin / Q000494:pharmacology; D006801:Humans; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance; D017720:Molecular Epidemiology; D009073:Mozambique; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D013420:Sulfamethoxazole / Q000494:pharmacology; D013753:Tetracycline Resistance; D014296:Trimethoprim Resistance; D001618:beta-Lactamases / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004917:Erythromycin; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D001618:beta-Lactamases; D013420:Sulfamethoxazole; D008712:Methicillin