An improved method of 90% pancreatectomy using a low dose of streptozotocin at the pancreaticoduodenal artery results in a rapid diabetic stage in dogs.
An improved method of inducing diabetes in dogs was developed. This method included 90% pancreatectomy, 2 mg/kg streptozotocin (STZ) perfused into pancreaticoduodenal artery, and the fixation suture of the duodenum to the costo-abdominal wall. Vasopressin injection administered to the animals before surgery reduced bleeding. All dogs used in this procedure survived and became diabetic. One month after the procedure the pancreatic islets were reduced in volume and the number compared with pancreas tissue obtained during the surgery. Acinar tissue remained with a normal histology, and exocrine function maintained the physiological parameters, except for a soft faecal consistency. We conclude that this procedure is effective in inducing experimental diabetes in dogs.
Morales
A Pimentel
AP
Center of Biomedical Research (CIBIOR), Mexican Institute of Social Security, Km. 4.5 Carretera Atlixco-Metepec, Mexico.
Conde
E Gómez
EG
López
M G Delgado
MG
Valle
M I Jiménez
MI
Díaz
J F Guerrero
JF
Jáuregui
P Hernández
PH
D000818:Animals; D001158:Arteries / Q000601:surgery*; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D004285:Dogs; D007261:Infusions, Intra-Arterial; D007515:Islets of Langerhans / Q000166:cytology; D010179:Pancreas / Q000098:blood supply; D010180:Pancreatectomy / Q000379:methods*; D013311:Streptozocin / Q000008:administration & dosage
D013311:Streptozocin