Dynamic localization and functional implications of Aurora-C kinase during male mouse meiosis.
Aurora-C was first identified during screening for kinases expressed in mouse sperm and eggs. Herein, we report for the first time the precise subcellular localization of endogenous Aurora-C during male meiotic division. The localization of Aurora-C was analyzed by immunofluorescence staining on chromosome spreads of mouse spermatocytes or in squashed seminiferous tubules. Aurora-C was first detected at clusters of chromocenters in diplotene spermatocytes and was concentrated at centromeres in metaphase I and II. Interestingly, Aurora-C was also found along the chromosome axes, including both the regions of centromeres and the chromosome arms in diakinesis. During the anaphase I/telophase I and anaphase II/telophase II transitions, Aurora-C was relocalized to the spindle midzone and midbody. A similar distribution pattern was also observed for Aurora-B during male meiotic divisions. Surprisingly, we detected no Aurora-C in mitotic spermatogonia. Furthermore, immunoprecipitation analyses revealed that INCENP associated with Aurora-C in the male testis. We propose that INCENP recruits Aurora-C (or some other factor(s) recruit INCENP and Aurora-C) to meiotic chromosomes, while Aurora-C may either work alone or cooperate with Aurora-B to regulate chromosome segregation during male meiosis.
Tang
Chieh-Ju C
CJ
Institute of Biomedical Sciences, Academia Sinica, 128 Yen-Chiu-Yuan Rd., Sec. 2, Taipei 11529, Taiwan.
Lin
Chun-Yi
CY
Tang
Tang K
TK
D000705:Anaphase; D000818:Animals; D064107:Aurora Kinase B; D064108:Aurora Kinase C; D064127:Aurora Kinases; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology; D002875:Chromosomes / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D047468:Immunoprecipitation; D008297:Male; D008540:Meiosis; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000378:metabolism; D012671:Seminiferous Tubules / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013093:Spermatogonia / Q000378:metabolism; D008941:Spindle Apparatus; D013692:Telophase; D013737:Testis / Q000378:metabolism
D002868:Chromosomal Proteins, Non-Histone; C513185:Incenp protein, mouse; C576924:Aurkb protein, mouse; C576928:Aurkc protein, mouse; D064107:Aurora Kinase B; D064108:Aurora Kinase C; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases