Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site.
The septins are GTP-binding, filament-forming proteins that are involved in cytokinesis and other processes. In the yeast Saccharomyces cerevisiae, the septins are recruited to the presumptive bud site at the cell cortex, where they form a ring through which the bud emerges. We report here that in wild-type cells, the septins typically become detectable in the vicinity of the bud site several minutes before ring formation, but the ring itself is the first distinct structure that forms. Septin recruitment depends on activated Cdc42p but not on the normal pathway for bud-site selection. Recruitment occurs in the absence of F-actin, but ring formation is delayed. Mutant phenotypes and suppression data suggest that the Cdc42p effectors Gic1p and Gic2p, previously implicated in polarization of the actin cytoskeleton, also function in septin recruitment. Two-hybrid, in vitro protein binding, and coimmunoprecipitation data indicate that this role involves a direct interaction of the Gic proteins with the septin Cdc12p.
Iwase
Masayuki
M
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6058, USA.
Luo
Jianying
J
Nagaraj
Satish
S
Longtine
Mark
M
Kim
Hyong Bai
HB
Haarer
Brian K
BK
Caruso
Carlo
C
Tong
Zongtian
Z
Pringle
John R
JR
Bi
Erfei
E
D000199:Actins / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013489:Suppression, Genetic; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / Q000172:deficiency / Q000378:metabolism*
D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; C073594:CDC24 protein, S cerevisiae; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C109252:GIC1 protein, S cerevisiae; C109253:GIC2 protein, S cerevisiae; D020662:Guanine Nucleotide Exchange Factors; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae