Derivation of human embryonic stem cells in defined conditions.
We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
Ludwig
Tenneille E
TE
WiCell Research Institute, PO Box 7365, Madison, Wisconsin 53707-7365, USA.
Levenstein
Mark E
ME
Jones
Jeffrey M
JM
Berggren
W Travis
WT
Mitchen
Erika R
ER
Frane
Jennifer L
JL
Crandall
Leann J
LJ
Daigh
Christine A
CA
Conard
Kevin R
KR
Piekarczyk
Marian S
MS
Llanas
Rachel A
RA
Thomson
James A
JA
D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
D003470:Culture Media