title: Sporadic methicillin resistance in community acquired Staphylococcus aureus in Mozambique.
abstract: This study reports the drug resistance and clonal relationship of 24 Staphylococcus aureus community acquired isolates from patients attending Maputo Central Hospital, Mozambique, during one year (2002-2003). All the isolates produced beta-lactamase, six strains were resistant to tetracycline alone, three were resistant to erythromycin alone and one was resistant to trimethoprim-sulfamethoxazole; 11 were susceptible to all other drugs tested. Only one strain showed a multiple resistance pattern, including methicillin resistance. To investigate the clonal relationships we applied the ERIC AP-PCR and the SmaI PFGE RFLP methods. Overlapping drug resistances with these two molecular profiles, no significant correlation was obtained. The emergence of methicillin resistance in a multiple resistant strain is of great concern for resistance spreading surveillance in Mozambique.
authors.affiliation: Dipartimento di Biologia Cellulare e Sviluppo, Università di Roma, La Sapienza, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Ceccarelli
authors.forename: José
authors.initials: J
authors.lastname: Mondlane
authors.forename: Marco
authors.initials: M
authors.lastname: Sale
authors.forename: Anna M
authors.initials: AM
authors.lastname: Salvia
authors.forename: Elena
authors.initials: E
authors.lastname: Folgosa
authors.forename: Piero
authors.initials: P
authors.lastname: Cappuccinelli
authors.forename: Mauro M
authors.initials: MM
authors.lastname: Colombo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017714:Community-Acquired Infections / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D024901:Drug Resistance, Multiple, Bacterial; D004917:Erythromycin / Q000494:pharmacology; D006801:Humans; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance; D017720:Molecular Epidemiology; D009073:Mozambique; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D013420:Sulfamethoxazole / Q000494:pharmacology; D013753:Tetracycline Resistance; D014296:Trimethoprim Resistance; D001618:beta-Lactamases / Q000096:biosynthesis
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004917:Erythromycin; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D001618:beta-Lactamases; D013420:Sulfamethoxazole; D008712:Methicillin