title: Pbcrk-1, the Plasmodium berghei orthologue of P. falciparum cdc-2 related kinase-1 (Pfcrk-1), is essential for completion of the intraerythrocytic asexual cycle.
abstract: The molecular mechanisms underlying gametocytogenesis in malaria parasites are not understood. Plasmodium falciparum cdc2-related kinase 1 (pfcrk-1), a gene that is expressed predominantly in gametocytes, bears homology to the PITSLRE subfamily of cyclin-dependent kinases and has been hypothesized to function as a negative regulator of the cell cycle. We attempted to knock-out pbcrk-1, the P. berghei orthologue of pfcrk-1, but were unable to recover P. berghei parasites with a disrupted pbcrk-1 locus. In contrast, an integration event at this locus that did not result in a loss-of-function of the pbcrk-1 gene was readily observed. This strongly suggests that a functional pbcrk-1 gene product is essential to intraerythrocytic asexual multiplication.
authors.affiliation: Department of Immunology and Infectious diseases, Harvard School of Public Health, Boston, MA 02115, USA.
authors.forename: Radha
authors.initials: R
authors.lastname: Rangarajan
authors.forename: Amy
authors.initials: A
authors.lastname: Bei
authors.forename: Natohya
authors.initials: N
authors.lastname: Henry
authors.forename: Marylin
authors.initials: M
authors.lastname: Madamet
authors.forename: Daniel
authors.initials: D
authors.lastname: Parzy
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Nivez
authors.forename: Christian
authors.initials: C
authors.lastname: Doerig
authors.forename: Ali
authors.initials: A
authors.lastname: Sultan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D042846:CDC2-CDC28 Kinases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D004912:Erythrocytes / Q000469:parasitology*; D017353:Gene Deletion; D008969:Molecular Sequence Data; D010962:Plasmodium berghei / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D016053:RNA, Protozoan / Q000032:analysis; D051381:Rats; D012100:Reproduction, Asexual / Q000502:physiology; D016415:Sequence Alignment
chemical_list: D016053:RNA, Protozoan; D042846:CDC2-CDC28 Kinases