[Study on BS-12 as a new probe for the determination of DNA by resonance light scattering].
A new method has been developed for the determination of DNA by resonance light scattering with dodecyl dimethyl betaine (BS-12) in aqueous solution as a new probe. At pH 9.3, the interactions of BS-12 and DNA gave strong RLS signals at 388.0 nm. Linear relationships were found between the enhanced intensity of RLS and the concentration of DNAs in the range 0.25-12.0 microg x mL(-1) for fsDNA and 0.25-11.0 microg x mL(-1) for ctDNA. The limits of detection were 0.15 ng x mL(-1) and 0.16 ng x mL(-1) for fsDNA and ctDNA, respectively. The method was applied to the determination of synthetic samples with satisfactory results.
Chen
Zhan-guang
ZG
Science College, Shantou University, Shantou 515063, China.
Liao
Xu-hong
XH
Han
Ya-li
YL
Yang
Ying-yi
YY
Lu
Feng
F
Ding
Wei-feng
WF
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods*
D000644:Quaternary Ammonium Compounds; C034730:zwittergent 3-12; D004247:DNA; C427203:calf thymus DNA