title: Comparison of hydroxyl radical generation in patients undergoing coronary artery bypass grafting with and without cardiopulmonary bypass.
abstract: We measured the hydroxyl radical (.OH) generation in fourteen patients undergoing coronary artery bypass grafting (CABG), of whom seven patients underwent on-pump CABG with cardiopulmonary bypass (CPB) and seven patients underwent off-pump CABG without CPB. To detect .OH generation, we measured the urinary excretion of .OH products of creatinine (Cr), creatol (CTL; 5-hydroxycreatinine) and methylguanidine (MG) with HPLC using the one point sampling and collected urine during and after the operation. The urinary CTL value corrected urinary Cr value of on-pump CABG significantly increased about 3-5 times from the beginning of CPB to 4 h after operation compared to the baseline value before CPB in both the collected urine and the one point sampling urine. The urinary MG/Cr value in both groups did not change significantly. Significantly increased .OH generation was found during and soon after on-pump CABG.
authors.affiliation: Graduated School of Comprehensive Human Sciences, University of Tsukuba, and Tsukuba Medical Center Hospital, Department of Cardiovascular Surgery, Tsukuba, Japan.
authors.forename: Motoo
authors.initials: M
authors.lastname: Osaka
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Aoyagi
authors.forename: Akiko
authors.initials: A
authors.lastname: Hirakawa
authors.forename: Motoo
authors.initials: M
authors.lastname: Nakajima
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Jikuya
authors.forename: Osamu
authors.initials: O
authors.lastname: Shigeta
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Sakakibara
mesh_terms: D000368:Aged; D002315:Cardiopulmonary Bypass; D002851:Chromatography, High Pressure Liquid; D001026:Coronary Artery Bypass; D003404:Creatinine / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism*; D008297:Male; D008760:Methylguanidine / Q000652:urine
chemical_list: C064402:creatol; D017665:Hydroxyl Radical; D008760:Methylguanidine; D003404:Creatinine