title: Rapid responses to thyroxine in the testis: active protein synthesis-independent pathway.
abstract: We investigated the involvement of protein synthesis in the stimulatory action of thyroid hormones on amino acid accumulation and characterized K(+) currents involved in the hyperpolarizing effect of thyroxine (T(4)) on Sertoli cells. Immature rat testes were incubated in Krebs Ringer-bicarbonate buffer (KRb) in the presence of [(14)C]methylaminoisobutyric acid with and without T(4), 3,5,3'-l-triiodothyronine (T(3)) and/or cycloheximide. Sertoli cells were monitored by intracellular recording in a chamber perfused with KRb with and without T(4), T(3) and/or blockers, and the membrane potential was monitored. T(4) and T(3) stimulated amino acid accumulation and protein synthesis. Treatment with cycloheximide diminished T(3) stimulatory actions on amino acid accumulation but had no effect on T(4) action. Both hormones elicited a hyperpolarization of the Sertoli cell membrane potential which involved K(+) channels, since TEA and apamin abolished this effect. These findings on rapid membrane actions of thyroid hormone in the testis suggest that some effects of T(4) are modulated by non-genomic mechanisms.
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis-Santa Catarina, CEP 88010-970, Brazil. danusa_menegaz@yahoo.com.br
authors.forename: D
authors.initials: D
authors.lastname: Menegaz
authors.forename: A
authors.initials: A
authors.lastname: Zamoner
authors.forename: C
authors.initials: C
authors.lastname: Royer
authors.forename: L D
authors.initials: LD
authors.lastname: Leite
authors.forename: Z A
authors.initials: ZA
authors.lastname: Bortolotto
authors.forename: F R M B
authors.initials: FR
authors.lastname: Silva
mesh_terms: D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004594:Electrophysiology; D008297:Male; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013737:Testis / Q000187:drug effects* / Q000502:physiology*; D013974:Thyroxine / Q000494:pharmacology*; D013997:Time Factors; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D011506:Proteins; D014284:Triiodothyronine; D003513:Cycloheximide; D013974:Thyroxine