title: Crosslinked poly(epsilon-caprolactone/D,L-lactide)/bioactive glass composite scaffolds for bone tissue engineering.
abstract: A series of elastic polymer and composite scaffolds for bone tissue engineering applications were designed. Two crosslinked copolymer matrices with 90/10 and 30/70 mol % of epsilon-caprolactone (CL) and D,L-lactide (DLLA) were prepared with porosities from 45 to 85 vol % and their mechanical and degradation properties were tested. Corresponding composite scaffolds with 20-50 wt % of particulate bioactive glass (BAG) were also characterized. Compressive modulus of polymer scaffolds ranged from 190+/-10 to 900+/-90 kPa. Lactide rich scaffolds absorbed up to 290 wt % of water in 4 weeks and mainly lost their mechanical properties. Caprolactone rich scaffolds absorbed no more than 110 wt % of water in 12 weeks and kept their mechanical integrity. Polymer and composite scaffolds prepared with P(CL/DLLA 90/10) matrix and 60 vol % porosity were further analyzed in simulated body fluid and in osteoblast culture. Cell growth was compromised inside the 2 mm thick three-dimensional scaffold specimens as a static culture model was used. However, composite scaffolds with BAG showed increased osteoblast adhesion and mineralization when compared to neat polymer scaffolds.
authors.affiliation: Department of Prosthetic Dentistry and Biomaterials Science, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, FI-20520 Turku, Finland. ville.meretoja@utu.fi
authors.forename: V V
authors.initials: VV
authors.lastname: Meretoja
authors.forename: A O
authors.initials: AO
authors.lastname: Helminen
authors.forename: J J
authors.initials: JJ
authors.lastname: Korventausta
authors.forename: V
authors.initials: V
authors.lastname: Haapa-aho
authors.forename: J V
authors.initials: JV
authors.lastname: Seppälä
authors.forename: T O
authors.initials: TO
authors.lastname: Närhi
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001826:Body Fluids / Q000737:chemistry; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D018786:Bone Substitutes; D003432:Cross-Linking Reagents; D011091:Polyesters; C505727:poly(epsilon-caprolactone-co-lactide)