Proteomic analysis of the heat shock response in Synechocystis PCC6803 and a thermally tolerant knockout strain lacking the histidine kinase 34 gene.
Proteomic analysis of the heat shock response of wild type and a mutant of the histidine kinase 34 gene (Deltahik34), which shows increased thermal tolerance, has been performed in the cyanobacterium Synechocystis sp. PCC6803. In vivo radioactive labelling demonstrates that major proteomic changes occur within 1 h of heat shock. 2-D DIGE and MS have been used to quantify changes in specific proteins following heat shock in the wild type and the mutant. Over 100 spots, corresponding to 65 different proteins alter following heat shock. Changes occur not only in the classical heat shock proteins but also in the protein biosynthetic machinery, amino acid biosynthetic enzymes, components of the light and dark acts of photosynthesis and energy metabolism. The Deltahik34 cells have elevated levels of heat shock proteins under both non-heat shock and heat shock conditions, in comparison to the wild type, consistent with Hik34, or a down stream component, being a negative regulator of heat shock-responsive genes.
Slabas
Antoni R
AR
School of Biological and Biomedical Sciences, University of Durham, Durham, UK. a.r.slabas@durham.ac.uk
Suzuki
Iwane
I
Murata
Norio
N
Simon
William J
WJ
Hall
John J
JJ
D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000235:genetics; D018869:Heat-Shock Response / Q000502:physiology*; D000071677:Histidine Kinase; D009154:Mutation; D011494:Protein Kinases / Q000172:deficiency*; D040901:Proteomics; D017384:Sequence Deletion; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D046939:Synechocystis / Q000737:chemistry* / Q000201:enzymology
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D011494:Protein Kinases; D000071677:Histidine Kinase