Soft lithographic patterning of supported lipid bilayers onto a surface and inside microfluidic channels.
We present simple soft lithographic methods for patterning supported lipid bilayer (SLB) membranes onto a surface and inside microfluidic channels. Micropatterns of polyethylene glycol (PEG)-based polymers were fabricated on glass substrates by microcontact printing or capillary moulding. The patterned PEG surfaces have shown 97 +/- 0.5% reduction in lipid adsorption onto two dimensional surfaces and 95 +/- 1.2% reduction inside microfluidic channels in comparison to glass control. Atomic force microscopy measurements indicated that the deposition of lipid vesicles led to the formation of SLB membranes by vesicle fusion due to hydrophilic interactions with the exposed substrate. Furthermore, the functionality of the patterned SLBs was tested by measuring the binding interactions between biotin (ligand)-labeled lipid bilayer and streptavidin (receptor). SLB arrays were fabricated with spatial resolution down to approximately 500 nm on flat substrate and approximately 1 microm inside microfluidic channels, respectively.
Kim
Pilnam
P
School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151-742, Korea.
Lee
Sang Eun
SE
Jung
Ho Sup
HS
Lee
Hea Yeon
HY
Kawai
Tomoji
T
Suh
Kahp Y
KY
D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008422:Materials Testing; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D013499:Surface Properties
D004129:Dimethylpolysiloxanes; D008051:Lipid Bilayers