Cloning and distribution of the bullfrog type 1 and type 2 corticotropin-releasing factor receptors.
The molecular cloning of bullfrog cDNAs encoding receptors for corticotropin-releasing factor (CRF) was undertaken as the first step in our investigation of the action of CRF at the receptor level. The specific cDNAs (630 bp) were amplified by reverse-transcribed PCR of a total RNA isolated from hypothalamus of the bullfrog, Rana catesbeiana. With the partial cDNAs as probes, two types of full-length CRF receptor cDNAs (type 1: 2275 bp, type 2: 1600 bp) were obtained from a hypothalamic cDNA library. The deduced amino acid sequences of CRF receptor types 1 (CRFR1) and CRF receptor type 2 (CRFR2) showed 79-94% and 81-92% identity with CRFR1 and CRFR2 of other vertebrates, respectively. The distribution of mRNAs of the two CRF receptors was studied by RT-PCR analysis. CRFR1 mRNA was detected almost exclusively in brain and pituitary, and to a very minor degree in testis, whereas CRFR2 was found in the peripheral tissues as well as in the brain and pituitary.
Ito
Yoichi
Y
Department of Biology, School of Education, Waseda University, Nishiwaseda 1-6-1, Shinjuku-ku, Tokyo 169-8050, Japan.
Okada
Reiko
R
Noriyuki
Takahashi
T
Kikuyama
Sakaé
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D007031:Hypothalamus / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D011892:Rana catesbeiana / Q000235:genetics*; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics*; D014018:Tissue Distribution; D000099052:CRF Receptor, Type 1
C101531:CRF receptor type 2; D018019:Receptors, Corticotropin-Releasing Hormone; D000099052:CRF Receptor, Type 1