A selective turn-on fluorescent sensor for imaging copper in living cells.
We present the synthesis, properties, and biological applications of Coppersensor-1 (CS1), a new water-soluble, turn-on fluorescent sensor for intracellular imaging of copper in living biological samples. CS1 utilizes a BODIPY reporter and thioether-rich receptor to provide high selectivity and sensitivity for Cu+ over other biologically relevant metal ions, including Cu2+, in aqueous solution. This BODIPY-based probe is the first Cu+-responsive sensor with visible excitation and emission profiles and gives a 10-fold turn-on response for detecting this ion. Confocal microscopy experiments further establish that CS1 is membrane-permeable and can successfully monitor intracellular Cu+ levels within living cells.
Zeng
Li
L
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Miller
Evan W
EW
Pralle
Arnd
A
Isacoff
Ehud Y
EY
Chang
Christopher J
CJ
D015374:Biosensing Techniques / Q000379:methods*; D001896:Boron Compounds / Q000737:chemistry; D002460:Cell Line; D003300:Copper / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D055095:Optics and Photonics; D013050:Spectrometry, Fluorescence
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D003300:Copper