Alterations of renal phenotype and gene expression profiles due to protein overload in NOD-related mouse strains.
BACKGROUND
Despite multiple causes, Chronic Kidney Disease is commonly associated with proteinuria. A previous study on Non Obese Diabetic mice (NOD), which spontaneously develop type 1 diabetes, described histological and gene expression changes incurred by diabetes in the kidney. Because proteinuria is coincident to diabetes, the effects of proteinuria are difficult to distinguish from those of other factors such as hyperglycemia. Proteinuria can nevertheless be induced in mice by peritoneal injection of Bovine Serum Albumin (BSA). To gain more information on the specific effects of proteinuria, this study addresses renal changes in diabetes resistant NOD-related mouse strains (NON and NOD.B10) that were made to develop proteinuria by BSA overload.


METHODS
Proteinuria was induced by protein overload on NON and NOD.B10 mouse strains and histology and microarray technology were used to follow the kidney response. The effects of proteinuria were assessed and subsequently compared to changes that were observed in a prior study on NOD diabetic nephropathy.


RESULTS
Overload treatment significantly modified the renal phenotype and out of 5760 clones screened, 21 and 7 kidney transcripts were respectively altered in the NON and NOD.B10. Upregulated transcripts encoded signal transduction genes, as well as markers for inflammation (Calmodulin kinase beta). Down-regulated transcripts included FKBP52 which was also down-regulated in diabetic NOD kidney. Comparison of transcripts altered by proteinuria to those altered by diabetes identified mannosidase 2 alpha 1 as being more specifically induced by proteinuria.


CONCLUSIONS
By simulating a component of diabetes, and looking at the global response on mice resistant to the disease, by virtue of a small genetic difference, we were able to identify key factors in disease progression. This suggests the power of this approach in unraveling multifactorial disease processes.
Wilson
Karen H S
KH
Center for Biotechnology and Genomic Medicine, Medical College of Georgia, PV6B108, Augusta, GA 30912-2400, USA. Karen.wilson@kmf.gu.se
McIndoe
Richard A
RA
Eckenrode
Sarah
S
Morel
Laurence
L
Agarwal
Anupam
A
Croker
Byron P
BP
She
Jin-Xiong
JX
D000818:Animals; D003920:Diabetes Mellitus / Q000209:etiology; D003928:Diabetic Nephropathies / Q000150:complications; D004198:Disease Susceptibility; D015870:Gene Expression; D020869:Gene Expression Profiling; D007668:Kidney / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D051379:Mice; D016688:Mice, Inbred NOD; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011507:Proteinuria / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D012710:Serum Albumin, Bovine
D012710:Serum Albumin, Bovine