Selenium species determination in selenium-enriched pumpkin (Cucurbita pepo L.) seeds by HPLC-UV-HG-AFS.
Pumpkins were treated by spraying the leaves in the flowering period with a water solution containing 1.5 mg Se per liter in the form of Na2SeO4. The average total selenium content of seeds was found to be 0.19 microg g(-1) in nontreated pumpkins and 1.1 microg g(-1) in exposed ones. For speciation analysis, enzymatic hydrolysis with different amounts of Protease XIV was carried out. Under optimal conditions of enzymatic hydrolysis, 90% of the total selenium was found in soluble forms. Separation of species was performed using HPLC on anion and cation exchange columns and for detection UVHG-AFS was applied. In enzymatic hydrolysis extracts, the main fraction of selenium was bound as selenomethionine (SeMet), representing on average of 81 +/- 8% of the total Se content in the sample.
Smrkolj
Polona
P
Jozef Stefan Institute, Jamova 39, 1111 Ljubljana, Slovenia.
Stibilj
Vekoslava
V
Kreft
Ivan
I
Kapolna
Emese
E
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D028464:Cucurbita / Q000737:chemistry*; D006868:Hydrolysis; D012643:Selenium / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D012643:Selenium