title: Phytotoxic components produced by pathogenic Fusarium against morning glory.
abstract: A pathogenic isolate of Fusarium, F. oxysporum f. sp. batatas O-17 (PF), causes wilt disease in leaf etiolation in sweet potato (Ipomoea batatas) and morning glory (Ipomoea tricolor). Extracts from PF cultures were screened for phytotoxic components using a growth inhibition assay with morning glory seedlings. The extracts were fractionated using differential solvent extraction and two active compounds, ergosterol and fusalanipyrone, were isolated from the less-polar fraction. Growth inhibition of morning glory seedlings showed a sigmoidal dose-response relationship, with fusalanipyrone exhibiting a two order of magnitude higher EC50 value than ergosterol (18 nM and 1.6 microM, respectively). Both compounds showed lower growth inhibition activity towards lettuce seedlings (Lactuca sativa). This study provides information on the phytotoxic components of PF and discusses the mechanism behind PFf-induced phytotoxicity.
authors.affiliation: Graduate School of Agriculture, Kyoto University, Japan. bsimz@scl.kyoto-u.ac.jp
authors.forename: Bun-ichi
authors.initials: B
authors.lastname: Shimizu
authors.forename: Fukuko
authors.initials: F
authors.lastname: Saito
authors.forename: Hisahi
authors.initials: H
authors.lastname: Miyagawa
authors.forename: Ken
authors.initials: K
authors.lastname: Watanabe
authors.forename: Tamio
authors.initials: T
authors.lastname: Ueno
authors.forename: Kanzo
authors.initials: K
authors.lastname: Sakata
authors.forename: Kei
authors.initials: K
authors.lastname: Ogawa
mesh_terms: D004875:Ergosterol / Q000032:analysis / Q000494:pharmacology; D005670:Fusarium / Q000472:pathogenicity*; D027741:Ipomoea / Q000187:drug effects / Q000382:microbiology*; D009183:Mycotoxins / Q000032:analysis* / Q000494:pharmacology; D010935:Plant Diseases / Q000382:microbiology*
chemical_list: D009183:Mycotoxins; D004875:Ergosterol