title: Reverse transcriptase domain sequences from Mungbean (Vigna radiata) LTR retrotransposons: sequence characterization and phylogenetic analysis.
abstract: The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were amplified from mungbean (Vigna radiata) genome using degenerate primers, cloned and sequenced. Among these 34% and 65% of respective clones of copia and gypsy RT sequences possessed stop codons or frame-shifts or both. The RT sequences corresponding to both the groups exhibit significant levels of heterogeneity. Presence of mungbean copia and gypsy RT sequences in other papilionoid legumes of the same (Phaseoleae) and different lineages (Loteae, Trifoleae, Cicereae) indicates existence of these elements prior to the radiation of papilionoid legumes and also supports the recent interpretations of close relationship between Phaseoleae and Loteae tribes of Papilionoideae subfamily. On the other hand significant homologies of some mungbean copia as well as gypsy RT sequences with those of unrelated plant species suggest their origin from different plant lineages and also that heterogeneous population of related elements were already existed throughout (even before the divergence of monocot and dicot) the evolution of these genera from their common ancestor.
authors.affiliation: Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, Suwon, Kyunggi 441-707, Republic of Korea.
authors.forename: Anupam
authors.initials: A
authors.lastname: Dixit
authors.forename: Kyung-Ho
authors.initials: KH
authors.lastname: Ma
authors.forename: Jae-Woong
authors.initials: JW
authors.lastname: Yu
authors.forename: Eun-Gi
authors.initials: EG
authors.lastname: Cho
authors.forename: Yong-Jin
authors.initials: YJ
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D019143:Evolution, Molecular; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics*; D018626:Retroelements / Q000235:genetics*; D020079:Terminal Repeat Sequences / Q000235:genetics*
chemical_list: D018626:Retroelements; D012194:RNA-Directed DNA Polymerase