title: T-kininogen induces endothelial cell proliferation.
abstract: Basal proliferation of endothelial cells increases with age, and this might play a role in the etiology of age-related vascular diseases, as well as angiogenesis. Serum kininogen levels increase during aging in rats and humans, and T-kininogen (T-KG) can affect proliferative homeostasis in several cell models. Both kinins and kininogens have been shown previously to be angiogenic through activation of endothelial cell proliferation, and here we show that exposure of endothelial cells to T-KG results in vigorous cell proliferation, accompanied by ERK/AKT activation. In our experiments, the proliferative response requires B1 and B2 kinin receptors, even though kinins are not released from the precursor. We hypothesize that the age-related increase in T-KG could play a significant role in the age-related dysregulation of vascular physiology and function.
authors.affiliation: Instituto de Ciencias Biomédicas, Programa de Biología Celular y Molecular and Centro FONDAP de Estudios Moleculares de la Célula, Facultad de Medicina, Universidad de Chile, Independencia 1027, Santiago, Chile.
authors.forename: Viviana
authors.initials: V
authors.lastname: Pérez
authors.forename: Elías
authors.initials: E
authors.lastname: Leiva-Salcedo
authors.forename: Claudio
authors.initials: C
authors.lastname: Acuña-Castillo
authors.forename: Mauricio
authors.initials: M
authors.lastname: Aravena
authors.forename: Christian
authors.initials: C
authors.lastname: Gómez
authors.forename: Valeria
authors.initials: V
authors.lastname: Sabaj
authors.forename: Alicia
authors.initials: A
authors.lastname: Colombo
authors.forename: Sumiyo
authors.initials: S
authors.lastname: Nishimura
authors.forename: Claudio
authors.initials: C
authors.lastname: Pérez
authors.forename: Robin
authors.initials: R
authors.lastname: Walter
authors.forename: Felipe
authors.initials: F
authors.lastname: Sierra
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D007704:Kininogens / Q000378:metabolism / Q000502:physiology*; D051058:Oncogene Protein v-akt / Q000378:metabolism; D051381:Rats; D018000:Receptors, Peptide / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology
chemical_list: D007704:Kininogens; D018000:Receptors, Peptide; C044865:kininogen receptor; D051058:Oncogene Protein v-akt; D048049:Extracellular Signal-Regulated MAP Kinases