title: Site-specific DNA excision in transgenic rice with a cell-permeable cre recombinase.
abstract: The removal of selected marker genes from transgenic plants is necessary to address biosafety concerns and to carry out further experiments with transgenic organisms. In the present study, the 12-amino-acid membrane translocation sequence (MTS) from the Kaposi fibroblast growth factor (FGF)-4 was used as a carrier to deliver enzymatically active Cre proteins into living plant cells, and to produce a site-specific DNA excision in transgenic rice plants. The process, which made cells permeable to Cre recombinase-mediated DNA recombination, circumvented the need to express Cre under spatiotemporal control and was proved to be a simple and efficient system to achieve marker-free transgenic plants. The ultimate aim of the present study is to develop commercial rice cultivars free from selected marker genes to hasten public acceptance of transgenic crops.
authors.affiliation: National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, People's Republic of China.
authors.forename: Ming-Xia
authors.initials: MX
authors.lastname: Cao
authors.forename: Jian-Qiu
authors.initials: JQ
authors.lastname: Huang
authors.forename: Quan-Hong
authors.initials: QH
authors.lastname: Yao
authors.forename: Sheng-Jun
authors.initials: SJ
authors.lastname: Liu
authors.forename: Cheng-Long
authors.initials: CL
authors.lastname: Wang
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Wei
mesh_terms: D015139:Blotting, Southern; D002478:Cells, Cultured; D002934:Cinnamates / Q000494:pharmacology; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D051519:Fibroblast Growth Factor 4 / Q000235:genetics; D017353:Gene Deletion; D015870:Gene Expression / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D019913:Nuclear Localization Signals / Q000235:genetics; D012275:Oryza / Q000166:cytology / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D011995:Recombination, Genetic / Q000187:drug effects / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D002934:Cinnamates; D018744:DNA, Plant; D051519:Fibroblast Growth Factor 4; D019913:Nuclear Localization Signals; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D006921:Hygromycin B; C026273:hygromycin A; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase; C045073:Cre recombinase; D019426:Integrases; D005966:Glucuronidase