Inhibitory effect of Thai plant extracts on P-glycoprotein mediated efflux.
Curcuminoids from Curcuma longa L. and extracts of Psidium guajava L., Andrographis paniculata (Burm. f.) Nees, Phyllanthus emblica L. and Solanum trilobatum L. were investigated for their inhibitory effect on P-glycoprotein (P-gp) on the efflux transport of rhodamine 123 (Rho-123 ) in Caco-2 cells and rat ileum. Of the five tested samples, curcuminoids and an extract of P. guajava showed the highest inhibitory effect on P-gp mediated efflux of Rho-123 in Caco-2 cells. Additionally, they were found to have equal potential in inhibiting Rho-123 efflux transport from serosal to mucosal surfaces of the rat ileum.
Junyaprasert
Varaporn Buraphacheep
VB
Department of Pharmacy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayutthaya, Rajathavee, Bangkok 10400, Thailand. pyvbp@mahidol.ac.th
Soonthornchareonnon
Noppamas
N
Thongpraditchote
Suchitra
S
Murakami
Teruo
T
Takano
Mikihisa
M
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000187:drug effects / Q000378:metabolism*; D035685:Andrographis / Q000737:chemistry; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D018938:Caco-2 Cells; D030024:Curcuma / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D008297:Male; D026901:Membrane Transport Proteins / Q000187:drug effects*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D029842:Phyllanthus emblica / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D059808:Polyphenols; D031647:Psidium / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D020112:Rhodamine 123 / Q000378:metabolism; D032322:Solanum / Q000737:chemistry; D013785:Thailand
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D005419:Flavonoids; D026901:Membrane Transport Proteins; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D020112:Rhodamine 123