title: The usefulness of immunostaining in washed cell cytological smears for differential diagnosis of B cell type malignant lymphoma.
abstract: We investigated the usefulness of immunostaining on washed cell cytological smears for the differential diagnosis of B-cell type malignant lymphoma. Twenty-eight cases with possible malignant lymphoma were examined. The tissues were squashed in a test tube of isotonic saline. The cell suspensions were then washed to remove non-specific soluble immmunoglobulin, and were smeared. Immunocytochemical staining for IgG, IgA, IgM, kappa and lambda was performed on the smears. As seen in the results, all cases of B cell lymphoma have a positive rate of 20% or over for kappa or lambda, and a kappa/lambda ratio of over 4.5 or a ratio of over 2.0 lambda/kappa, in the washed cell smears. Thus, it is possible to distinguish B cell lymphoma from the others by means of the results use as cut off scores. In addition, the rapid staining protocol in the present study was able to provide the results within 15 min. Therefore, it might be the most suitable method for the detection of immunoglobulin monoclonality in general laboratories.
authors.affiliation: Department of Pathology, Tsuchiura Kyodo General Hospital, Tsuchiura 300-0053.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ikeda
authors.forename: Naoya
authors.initials: N
authors.lastname: Funakoshi
authors.forename: Toshikatsu
authors.initials: T
authors.lastname: Shibata
mesh_terms: D003937:Diagnosis, Differential; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000276:immunology*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D007136:Immunoglobulins