Quantitation of O6-methylguanine-DNA methyltransferase gene messenger RNA in gliomas by means of real-time RT-PCR and clinical response to nitrosoureas.
1. O6-methylguanine-DNA methyltransferase (MGMT) mRNA was measured in 50 malignant gliomas that had received 1-(4-amino-2-methyl-5-pyrimidynyl) methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride (ACNU) after the resection of the tumor by real-time reverse transcription-polymerase chain reaction (RT-PCR) using TaqMan probe. 2. The mean absolute value of MGMTmRNA normalized to the level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for 50 tumors was 1.29 x 10(4)+/- 1.28 x 10(4) copy/microg RNA (mean +/- SD). The amount of MGMTmRNA less than 6 x 10(3) copy/microg RNA was the most significant factor in predicting the initial effect of treatment with ACNU by multi-variant regression analysis (p = 0.0157). 3. These results suggest that quantitation of MGMTmRNA is the excellent method for predicting for the effect of ACNU in glioma therapy.
Tanaka
Satoshi
S
Department of Neurosurgery, The Kitasato Institute Medical Center Hospital, 6-100 Arai, Kitamoto, Saitama, 364-8501, Japan. tanaka-s@kitasato.or.jp
Oka
Hidehiro
H
Fujii
Kiyotaka
K
Watanabe
Kaoru
K
Nagao
Kumi
K
Kakimoto
Atsushi
A
D000328:Adult; D001932:Brain Neoplasms / Q000188:drug therapy / Q000201:enzymology; D005910:Glioma / Q000188:drug therapy / Q000201:enzymology; D006801:Humans; D008875:Middle Aged; D009607:Nitrosourea Compounds / Q000627:therapeutic use*; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism*
D009607:Nitrosourea Compounds; D012333:RNA, Messenger; D019853:O(6)-Methylguanine-DNA Methyltransferase