Altered growth and polyamine catabolism following exposure of the chocolate spot pathogen Botrytis fabae to the essential oil of Ocimum basilicum.
Biomass of the fungal pathogen Botrytis fabae in liquid culture amended with two chemotypes of the essential oil of basil, Ocimum basilicum, was reduced significantly at concentrations of 50 ppm or less. The methyl chavicol chemotype oil increased the activity of the polyamine biosynthetic enzyme S-adenosylmethionine decarboxylase (AdoMetDC), but polyamine concentrations were not significantly altered. In contrast, the linalol chemotype oil decreased AdoMetDC activity in B. fabae, although again polyamine concentrations were not altered significantly. However activities of the polyamine catabolic enzymes diamine oxidase (DAO) and polyamine oxidase (PAO) were increased significantly in B. fabae grown in the presence of the essential oil of the two chemotypes. It is suggested that the elevated activities of DAO and PAO may be responsible, in part, for the antifungal effects of the basil oil, possibly via the generation of hydrogen peroxide and the subsequent triggering of programmed cell death.
Oxenham
Senga K
SK
Scottish Agricultural College, Ayr Campus, Auchincruive Estate, Ayr KA6 5HW, UK.
Svoboda
Katja P
KP
Walters
Dale R
DR
D012437:Adenosylmethionine Decarboxylase / Q000032:analysis; D000081248:Allylbenzene Derivatives; D006631:Amine Oxidase (Copper-Containing) / Q000032:analysis; D000840:Anisoles / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D017209:Apoptosis; D018533:Biomass; D020171:Botrytis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D018649:Ocimum basilicum / Q000737:chemistry*; D009822:Oils, Volatile / Q000494:pharmacology*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D011073:Polyamines / Q000378:metabolism*; D000097667:Polyamine Oxidase
D012437:Adenosylmethionine Decarboxylase; D000081248:Allylbenzene Derivatives; D006631:Amine Oxidase (Copper-Containing); D000840:Anisoles; D000935:Antifungal Agents; D006861:Hydrogen Peroxide; D009822:Oils, Volatile; D000587:Oxidoreductases Acting on CH-NH Group Donors; D010936:Plant Extracts; D011073:Polyamines; D000097667:Polyamine Oxidase; C007633:estragole