title: An aqueous-organic two-phase bioprocess for efficient production of the natural aroma chemicals 2-phenylethanol and 2-phenylethylacetate with yeast.
abstract: The natural aroma chemicals 2-phenylethanol (2-PE) and 2-phenylethylacetate (2-PEAc) are of high industrial relevance and can be produced from L-phenylalanine in a yeast-based process with growth-associated product formation. Due to product inhibition, in situ product removal is mandatory to obtain economically interesting concentrations. A fed-batch approach using polypropylene glycol 1200 as in situ extractant and the precursor in a saturated concentration led to the highest 2-PE productivity reported for a bioprocess so far. With Kluyveromyces marxianus CBS 600, 26.5 g/l 2-PE and 6.1 g/l 2-PEAc in the organic phase were obtained, corresponding to space-time yields of 0.33 and 0.08 g/l h, respectively.
authors.affiliation: Dechema e.V, Karl-Winnacker-Institut, Biochemical Engineering Group, Theodor-Heuss-Allee 25, 60486, Frankfurt am Main, Germany.
authors.forename: M M W
authors.initials: MM
authors.lastname: Etschmann
authors.forename: J
authors.initials: J
authors.lastname: Schrader
mesh_terms: D000085:Acetates / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D007716:Kluyveromyces / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry
chemical_list: D000085:Acetates; D011108:Polymers; D011409:Propylene Glycols; C012504:polypropylene glycol; D010649:Phenylalanine; C054590:2-phenylethyl acetate; D010626:Phenylethyl Alcohol