Potentiation of antifungal activity of amphotericin B by essential oil from Cinnamomum cassia.
The antifungal activity of the essential oil from Cinnamomum cassia, alone or combined with amphotericin B, a drug widely used for most indications despite side-effects was investigated. The composition of the oil was analysed by GC/MS and characterized by its very high content of cinnamaldehyde (92.2%). The minimal inhibitory concentration (MIC 80%), used to evaluate the antifungal activity against Candida albicans, was determined by a macrobroth dilution method followed by a modelling of fungal growth. The essential oil of Cinnamomum cassia exhibited strong antifungal effect (MIC 80% = 0.169 microL/mL and K(aff) = 18,544 microL/mL). A decrease of the MIC 80% of amphotericin B was obtained when the culture medium contained essential oil concentrations ranging from 0.08 to 0.1 microL/mL. The strongest decrease (70%) was obtained when the medium contained 0.1 microL/mL of essential oil. This potentiation of amphotericin B obtained in vitro may show promise for the development of less toxic and more effective therapies especially for the treatment of HIV infection.
Giordani
R
R
Laboratoire de Botanique, Cryptogamie et Biologie Cellulaire, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France. rglatplus@aol.com
Regli
P
P
Kaloustian
J
J
Portugal
H
H
D000171:Acrolein / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D002177:Candidiasis / Q000188:drug therapy; D002934:Cinnamates / Q000302:isolation & purification; D032904:Cinnamomum aromaticum / Q000737:chemistry*; D004357:Drug Synergism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D012044:Regression Analysis
D000935:Antifungal Agents; D002934:Cinnamates; D009822:Oils, Volatile; D010936:Plant Extracts; C017910:2-methoxycinnamaldehyde; D000171:Acrolein; D000666:Amphotericin B; C012843:cinnamaldehyde