Spectroscopic characterization of 2-isopropylmalic acid-aluminum(III) complex.
Spectroscopic elucidation of a 2-isopropylmalic acid (2-iPMA)-aluminum(III) complex has been carried out using (1)H, (13)C and (27)Al NMR spectroscopy, diffusion-ordered NMR spectroscopy (DOSY) and electrospray ionization mass spectrometry (ESI-MS). 2-iPMA is secreted by Saccharomyces cerevisiae and can dissolve Al(III) in the culture medium. The (1)H chemical shift perturbation and (1)H DOSY clearly indicated the formation of the 2-iPMA-Al(III) complex. The measurements of (13)C and (27)Al NMR spectroscopy and ESI-MS demonstrated that the major form of a complex is comprised four 2-iPMA and two Al(III) species. This compound is expected to possess strong Al(III)-detoxification capability.
Tashiro
Mitsuru
M
Department of Chemistry, College of Science and Technology, Meisei University, Hino, Tokyo 191-8506, Japan.
Fujimoto
Takashi
T
Suzuki
Taisuke
T
Furihata
Kazuo
K
Machinami
Tomoya
T
Yoshimura
Etsuro
E
D017607:Aluminum Compounds / Q000737:chemistry*; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D008293:Malates / Q000737:chemistry*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
C502920:2-isopropylmalic acid; D017607:Aluminum Compounds; D002247:Carbon Isotopes; D008293:Malates; D009942:Organometallic Compounds