title: [Genetics subtypes and distribution of Seoul virus in Henan].
abstract: OBJECTIVE
  To investigate the sub-genotypes and distribution ot Seoul virus in Henan.
  
  
  METHODS
  Rodents were collected in the major epidemic areas and rats lungs were studied by indirect immunofluorescence assay. Partial M and S segments were amplified with nested reverse transcription-polymerase chain reaction using Hantavirus genotype-specific primers, sequenced, analyzed and compared with other known sequences.
  
  
  RESULTS
  The Hantavirus carried by Rattus norvegicus, Rattus flavipectus and Mus musculus were all belonged to Seoul virus in the main epidemic areas of Henan. We constructed two phylogenetic tree based on the partial M and S segment sequences while phylogenetic analysis distinguished three genetic subtypes (S1, S2 and S3). S1 and S3 were found main subtypes in Henan.
  
  
  CONCLUSIONS
  The results indicated that the genetic subtypes of Hantavirus were complicated and widely distributed in Henan.
authors.affiliation: College of Liffe Science, Xinjiang Shihezi University, Shihezi 823003, China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Yong-zhen
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Lin-hong
authors.initials: LH
authors.lastname: Li
authors.forename: Yan-ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Ai-mei
authors.initials: AM
authors.lastname: Zhang
authors.forename: Zong-yu
authors.initials: ZY
authors.lastname: Hao
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Sun
authors.forename: Hua-xin
authors.initials: HX
authors.lastname: Chen
mesh_terms: D000818:Animals; D002681:China; D010802:Phylogeny; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029263:Seoul virus / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA