Identification of basogranulin (BB1) as a novel immunohistochemical marker of basophils in normal bone marrow and patients with myeloproliferative disorders.
In myeloproliferative disorders (MPDs), basophils typically increase in number in the bone marrow (BM) and blood. In chronic myeloid leukemia (CML), basophilia is a diagnostic and prognostic variable. However, no reliable approach for routine detection and enumeration of basophils in BM sections is available. We applied the antibasogranulin antibody BB1 on paraffin-embedded BM sections in 21 control samples (normal BM), 45 patients with CML, 9 with chronic idiopathic myelofibrosis, 11 with polycythemia vera, 19 with essential thrombocythemia, and 7 with indolent systemic mastocytosis. As assessed by immunostaining of serial BM sections, BB1+ cells coexpressed myeloperoxidase, histidine decarboxylase, and leukosialin but did not express B- or T-cell-restricted antigens. BB1+ BM cells were found to be highly elevated in patients with CML compared with normal BM or other MPDs, with maximum counts found in accelerated phase CML (median, 160 cells/mm(2)). In summary, BB1 (basogranulin) is a new immunohistochemical basophil marker that should allow quantification of basophils in CML at diagnosis and during therapy.
Agis
Hermine
H
Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.
Krauth
Maria-Theresa
MT
Böhm
Alexandra
A
Mosberger
Isabella
I
Müllauer
Leonhard
L
Simonitsch-Klupp
Ingrid
I
Walls
Andrew F
AF
Horny
Hans-Peter
HP
Valent
Peter
P
D000328:Adult; D001491:Basophils / Q000737:chemistry*; D015415:Biomarkers; D001853:Bone Marrow / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000032:analysis*; D014157:Transcription Factors / Q000032:analysis*
D015415:Biomarkers; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D014157:Transcription Factors; C077914:BNC1 protein, human