Adsorption and fluctuations of giant liposomes studied by electrochemical impedance measurements.
The present study describes a novel approach based on electrochemical impedance measurements to follow the adsorption of giant liposomes on protein-coated solid surfaces with a time resolution in the order of seconds. The technical key features are circular gold-film electrodes as small as a few hundred micrometers in diameter and measurements of the electrode capacitance using AC signals in the kilohertz regime. Using Monte Carlo simulations, we were able to support the experiments and extract the rate constant of liposome adsorption. Besides monitoring the adsorption of liposomes on protein-coated surfaces, we also applied this technique to study shape fluctuations of the adsorbed vesicles and compared the corresponding power spectra with those recorded for hard particles and living animal cells.
Sapper
Angelika
A
Institut für Biochemie, Westfälische Wilhelms-Universität Münster, Wilhelm-Klemm-Strasse 2, 48149 Münster, Germany.
Reiss
Björn
B
Janshoff
Andreas
A
Wegener
Joachim
J
D000327:Adsorption; D000818:Animals; D002477:Cells / Q000166:cytology; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003198:Computer Simulation; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008422:Materials Testing; D009010:Monte Carlo Method; D011506:Proteins / Q000737:chemistry*; D013057:Spectrum Analysis; D013499:Surface Properties; D013997:Time Factors
D020099:Coated Materials, Biocompatible; D008081:Liposomes; D011506:Proteins; D006046:Gold