A comparative study of void volume markers in immobilized-artificial-membrane and reversed-phase liquid chromatography.
A comparative study of 10 void volume marker candidates on C(8), C(18), and IAM columns was described under several pH (3.0, 4.8, and 7.3) buffer conditions. Pycnometric analysis has been performed on the columns for comparison. In acidic conditions, small organic carboxylic acids suggested by the manufacturer did not necessarily give reliable and consistent estimates of the void volume for IAM column. However, changes in temperature and organic modifier composition led to small and predictable effects. The results presented here provide minimal but practical guidelines for the use of void volume markers in IAM chromatography.
Luo
Haibin
H
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
Cheng
Yuen-Kit
YK
D000096:Acetone / Q000302:isolation & purification; D001965:Bromides / Q000302:isolation & purification; D002264:Carboxylic Acids / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000379:methods*; D003596:Cytosine / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D009566:Nitrates / Q000302:isolation & purification; D017680:Potassium Compounds / Q000302:isolation & purification; D011193:Potassium Iodide / Q000302:isolation & purification; D013696:Temperature; D014498:Uracil / Q000302:isolation & purification
D001965:Bromides; D002264:Carboxylic Acids; D008567:Membranes, Artificial; D009566:Nitrates; D017680:Potassium Compounds; D000096:Acetone; D011193:Potassium Iodide; D014498:Uracil; D003596:Cytosine; C039004:potassium bromide