title: [Recipient lymphopenia state enhances the expansion and anti-leukemia effect of leukemia specific cytotoxic T lymphocytes].
abstract: OBJECTIVE
  To determine the role of recipient lymphopenia state in the expansion and function of leukemia specific cytotoxic T lymphocytes (CTLs).
  
  
  METHODS
  C57BL/6 mice were induced to lymphopenia with 6 Gy total body irradiation. Spleen T cells or leukemia specific T cells from EGFP+ transgenic C57BL/6-EGFP mice were adoptively transferred by intravenous injection. The mice were challenged subcutaneously with 1 x 10(6) FBL3 leukemic cells at day 2 after irradiation. The peripheral WBC count, percentage of EGFP+ cells, subsets of T cells and tumor sizes were monitored.
  
  
  RESULTS
  Both of the spleen T cell and leukemia specific CTL proliferated efficiently with the percentage of EGFP+ cells of 28. 81% and 42.24%, respectively, after infused into lymphopenic recipients. However, spleen T cells had no anti-leukemia effect regardless of its expansion. In contrast, leukemia specific CTLs showed a more rapid and extensive expansion under the condition of lymphopenia and a enhanced anti-leukemia immunity.
  
  
  CONCLUSIONS
  Transfusion of leukemia specific CTLs under lymphopenia state could be a feasible strategy to expand leukemia specific CTLs and generate favorable anti-leukemia effect in vivo.
authors.affiliation: Department of Hematology, Changhai Hospital of Second Military Medical College, Shanghai, China.
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Lou
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Xin-Yan
authors.initials: XY
authors.lastname: Jia
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zhang
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Wang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007938:Leukemia / Q000276:immunology*; D008231:Lymphopenia / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*