[Study on the changes in the distribution and chemical states of the hepatic intra-and extra-cellular sodium ion in the rats with severe burns at early stage].
OBJECTIVE
To investigate the changes in the distribution and chemical states of the hepatic intra- and extra-cellular sodium ion in the rats with severe burns, so as to provide guidance for fluid resuscitation at early postburn stage.


METHODS
Nineteen adult male Sprague-Dawley (SD) rats were employed in the study and were randomly divided into control (n = 12) and burn (n = 7) groups. The changes in the longitudinal (T1) and transverse (T2) relaxation times of hepatic intra-cellular and extra-cellular sodium in the two groups were studied with 23Na NMR spectroscopy and a shift reagent.


RESULTS
After infusion of the shift reagent,the extra-cellular sodium content in rat liver decreased by 17%, with obvious increase in fast T2 component (P < 0.01), indicating an increase in the fraction of Na+ binding sites in the extra-cellular space. The characteristics of relaxation of intra-cellular sodium remained unchanged despite a 57% increment in intra-cellular sodium content.


CONCLUSIONS
The deficiency of sodium as a permeable molecule might be related to the postburn movement of hypertonic sodium from extra-cellular to intra-cellular space. The results indicated that it is reasonable to administer high concentration of sodium in fluid resuscitation during the first 24 postburn hours.
Xia
Zhao-fan
ZF
Burn Center of PLA, Changhai Hospital, Second Military Medical University, Shanghai 200433, P. R. China.
Tian
Jian-guang
JG
Xiong
Jie
J
Ma
Bing
B
Wei
We
W
Wei
Duo
D
D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000503:physiopathology; D002412:Cations / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism*
D002412:Cations; D012964:Sodium