Oral single pulse exposure of flounder Platichthys flesus to 4-tert-octylphenol: Relations between tissue levels and estrogenic effects.
The accumulation of 4-tert-octylphenol and the associated estrogenic effects were studied after a single pulse exposure to flounder Platichthys flesus. 4-tert-octylphenol was administered orally in a single dose of 50 mg kg(-1) and tissue (liver, muscle and testis) and plasma concentrations of 4-tert-octylphenol as well as plasma vitellogenin were measured 3, 6, 12, 18, 24, 48, 72, 144 and 216 h after administration of the dose. Concentrations of 4-tert-octylphenol in plasma and tissues were determined by Liquid Chromatography Mass Spectrometry (LC-MS). 4-tert-octylphenol was detectable in liver, testis, muscle and plasma 3h post administration and an accumulation was observed in liver, muscle and plasma up to 12 h and in testis 18 h post administration, respectively. The maximum concentrations of 4-tert-octylphenol in liver, muscle and testis were 67, 3.2 and 6.8 microg g(-1), respectively. An increase in plasma vitellogenin levels was seen 48 h post administration and the vitellogenin level continued to increase (from <100 ng ml(-1) to 1.4 mg ml(-1)) until the end of the experiment 9 days after the administration of 4-tert-octylphenol.
Madsen
Louise L
LL
Institute of Biology, University of Southern Denmark, Odense, Campusvej 55, DK-5230 Odense M, Denmark. louisem@biology.sdu.dk
Korsgaard
Bodil
B
Bjerregaard
Poul
P
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D004967:Estrogens / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D005432:Flounder / Q000378:metabolism*; D008099:Liver / Q000737:chemistry; D008297:Male; D009132:Muscles / Q000737:chemistry; D010636:Phenols / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D013737:Testis / Q000737:chemistry; D013997:Time Factors; D014819:Vitellogenins / Q000096:biosynthesis / Q000097:blood*
D004967:Estrogens; D010636:Phenols; D014819:Vitellogenins; C105260:4-tert-octylphenol