Chiral high-performance liquid chromatographic separation of the enantiomers of tetrahydropalmatine and tetrahydroberberine, isolated from Corydalis yanhusuo.
HPLC methods have been developed for chiral resolution of the enantiomers of dl-tetrahydropalmatine (THP) and dl-tetrahydroberberine (THB), two active constituents of Corydalis yanhusuo W.T. Wang. On the analytical scale, good baseline separation of the enantiomers was achieved using cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phases in both normal-phase and polar organic modes. Validation of the analytical methods, including linearity, limits of detection, recovery, and precision, and semipreparative resolution of dl-THP and dl-THB, were achieved with methanol as mobile phase, without any basic additives, in polar organic mode using cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phases. On the semipreparative scale, small quantities of the individual enantiomers of THP and THB were isolated for study of the chiroptical properties of the individual enantiomers.
Zhai
Zong-De
ZD
Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, The Graduate School of Chinese Academy of Sciences, Lanzhou, 730000, PR China.
Shi
Yan-Ping
YP
Wu
Xiao-Mei
XM
Luo
Xing-Ping
XP
D001599:Berberine / Q000031:analogs & derivatives* / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031311:Corydalis / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D013237:Stereoisomerism
D001600:Berberine Alkaloids; D001599:Berberine; C014215:tetrahydropalmatine; C004645:canadine