title: UV photodissociation of phospho-seryl-containing peptides: laser stabilization of the phospho-seryl bond with multistage mass spectrometry.
abstract: Protonated precursor ions of phosphorylated peptides containing a tyrosyl residue have been subjected to UV laser-induced dissociation (LID) at a wavelength of 220 nm and to collision-induced dissociation (CID) in an ion trap. As expected, neutral loss of the phosphate group is one of the predominant fragmentation channels during CID together with H2O elimination. In contrast, LID leads mainly to the homolytic cleavage of the tyrosyl side chain and a restrained loss of the phosphate group. Interestingly, the intensity of the dephosphorylated fragment ion is greatly minimized when CID is carried out next on the radical precursor ion of the singly and doubly charged species.
authors.affiliation: Sciences Analytiques, UMR 5180 (Université Lyon I et CNRS), 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France. jerome.lemoine@univ-lyon1.fr
authors.forename: Jérôme
authors.initials: J
authors.lastname: Lemoine
authors.forename: Thibault
authors.initials: T
authors.lastname: Tabarin
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Antoine
authors.forename: Michel
authors.initials: M
authors.lastname: Broyer
authors.forename: Philippe
authors.initials: P
authors.lastname: Dugourd
mesh_terms: D000595:Amino Acid Sequence; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000737:chemistry* / Q000528:radiation effects*; D010766:Phosphorylation / Q000528:radiation effects; D010768:Phosphoserine / Q000737:chemistry*; D010777:Photochemistry; D014466:Ultraviolet Rays
chemical_list: D010748:Phosphopeptides; D010768:Phosphoserine