Effect of polychlorinated biphenyls (PCBs) on basal and OT-stimulated calcium concentrations in myometrial cells in cows.
We investigated the effect of PCB-77, -126 or -153 (10 or 100 ng/ml) on free intracellular calcium concentrations([Ca2+]i) in bovine myometrial cells from days 1-5 of the estrous cycle. Cells were incubated with or without PCBs for 48 h (38 degrees C, aerated atmosphere) and thereafter [Ca2+]i was measured by means of fluorescent calcium indicator Fura-2. PCBs increased basal concentrations of [Ca2+]i measured before oxytocin (OT) challenge. The increase in [Ca2+]i in cells incubated with PCBs and challenged with OT was inhibited or delayed when compared to control OT-stimulated cells (p<0.05). The applied doses of PCBs did not affect viability of myometrial cells. In conclusion, the influence of PCBs upon intracellular calcium mobilization in myometrial cells impaired the bovine uterus contractility.
Wróbel
Michał
M
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-718 Olsztyn-Kortowo, Poland.
Kotwica
Jan
J
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D030762:Estrous Cycle; D005260:Female; D009215:Myometrium / Q000166:cytology / Q000187:drug effects*; D010121:Oxytocin; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D014590:Uterine Contraction
D010121:Oxytocin; D011078:Polychlorinated Biphenyls; D002118:Calcium