title: Gold nanoparticles as carriers for efficient transmucosal insulin delivery.
abstract: Nanomaterials have gained tremendous importance in biology and medicine because they can be used as carriers for delivering small molecules such as drugs, proteins, and genes. We report herein the binding of the hormone insulin to gold nanoparticles and its application in transmucosal delivery for the therapeutic treatment of diabetes mellitus. Insulin was loaded onto bare gold nanoparticles and aspartic acid-capped gold nanoparticles and delivered in diabetic Wistar rats by both oral and intranasal (transmucosal) routes. Our principle observations are that there is a significant reduction of blood glucose levels (postprandial hyperglycemia) when insulin is delivered using gold nanoparticles as carriers by the transmucosal route in diabetic rats. Furthermore, control of postprandial hyperglycemia by the intranasal delivery protocol is comparable to that achieved using the standard subcutaneous administration used for type I diabetes mellitus, thus showing considerable promise for further development.
authors.affiliation: Nanoscience Group, Materials Chemistry Division, National Chemical Laboratory, Pune 411 008, India.
authors.forename: Hrushikesh M
authors.initials: HM
authors.lastname: Joshi
authors.forename: Devika R
authors.initials: DR
authors.lastname: Bhumkar
authors.forename: Kalpana
authors.initials: K
authors.lastname: Joshi
authors.forename: Varsha
authors.initials: V
authors.lastname: Pokharkar
authors.forename: Murali
authors.initials: M
authors.lastname: Sastry
mesh_terms: D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D002417:Cattle; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy; D004337:Drug Carriers; D005260:Female; D006046:Gold; D007328:Insulin / Q000008:administration & dosage*; D008297:Male; D053768:Metal Nanoparticles / Q000648:ultrastructure; D008854:Microscopy, Electron; D036103:Nanotechnology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004337:Drug Carriers; D007328:Insulin; D006046:Gold