title: Human cytomegalovirus UL131A, UL130 and UL128 genes are highly conserved among field isolates.
abstract: Coding sequences of the UL131A, UL130, and UL128 genes of human cytomegalovirus (HCMV) were found to be highly conserved among 34 field isolates from pregnant women with primary HCMV infection and their fetuses or newborns, as well as from solid organ transplant recipients and patients with AIDS. No strain clustering was observed. In contrast, sequencing of UL55 (gB coding gene) allowed the 34 isolates to be clustered into 4 genotypes. The conservation of the UL131A-UL128 locus is consistent with the conclusion that the three encoded proteins are all essential for growth of HCMV in endothelial cells and virus transfer to leukocytes.
authors.affiliation: Servizio di Virologia, IRCCS Policlinico San Matteo, Pavia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Baldanti
authors.forename: S
authors.initials: S
authors.lastname: Paolucci
authors.forename: G
authors.initials: G
authors.lastname: Campanini
authors.forename: A
authors.initials: A
authors.lastname: Sarasini
authors.forename: E
authors.initials: E
authors.lastname: Percivalle
authors.forename: M G
authors.initials: MG
authors.lastname: Revello
authors.forename: G
authors.initials: G
authors.lastname: Gerna
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000821:virology; D000595:Amino Acid Sequence; D016000:Cluster Analysis; D017124:Conserved Sequence; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D005814:Genes, Viral; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016377:Organ Transplantation; D010802:Phylogeny; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D008562:Membrane Glycoproteins; C502890:UL130 protein, human cytomegalovirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins