Simultaneous determination of 16 sulfonamides in honey by liquid chromatography/tandem mass spectrometry.
A method is described for the determination of 16 sulfonamides in honey. Samples are dissolved in phosphoric acid solution (pH2), cleaned up with 2 solid-phase extraction (SPE) cartridges, an aromatic sulfonic cation-exchange cartridge and an Oasis HLB SPE cartridge, and analyzed both qualitatively and quantitatively by liquid chromatography/tandem mass spectrometry (LC/MS/MS) under the selected conditions. Without exception, calibration curves were linear (r = > 0.995), when sulfamethizole was between 1.0 and 25.0 microg/kg; sulfacetamide, sulfapyridine, sulfadiazine, sulfachloropyridazine, sulfamethoxazole, sulfamerazine, sulfisoxazole, sulfamonomethoxine, and sulfadoxine were between 2.0 and 50.0 microg/kg; sulfamethoxypyridazine, sulfadimethoxine, and sulfathiazole were between 4.0 and 100.0 microg/kg; sulfamethazine and sulfameter were between 8.0 and 200.0 microg/kg; and sulfaphenazole was between 12.0 and 300.0 microg/kg. Average recoveries at 4 fortification levels in the range of 1.0-300 microg/kg in honey were 70.9-102.5%, and relative standard deviations were 2.02-11.52%. The limits of quantitation for the 16 sulfonamides were between 1.0 and 12.0 microg/kg, with the LC/MS/MS method.
Pang
Guo-Fang
GF
Qinhuangdao Entry-Exit Inspection and Quarantine Bureau, 39 Haibin Rd, Qinhuangdao, Hebei, PC 066002, People's Republic of China.
Cao
Yan-Zhong
YZ
Zhang
Jin-Jie
JJ
Jia
Guang-Qun
GQ
Fan
Chun-Lin
CL
Li
Xue-Min
XM
Liu
Yong-Ming
YM
Li
Zeng-Yin
ZY
Shi
Yu-Qiu
YQ
D002021:Buffers; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D005453:Fluorescence; D005504:Food Analysis / Q000379:methods*; D006722:Honey / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013449:Sulfonamides / Q000032:analysis* / Q000145:classification*
D002021:Buffers; D013449:Sulfonamides