Electrohydrodynamic jetting of mouse neuronal cells.
CAD (Cath.a-differentiated) cells, a mouse neuronal cell line, were subjected to electrohydrodynamic jetting at a field strength of 0.47-0.67 kV/mm, corresponding to an applied voltage of 7-10 kV. After jetting, the cells appeared normal and continued to divide at rates similar to those shown by control samples. Jetted cells, when placed in serum-free medium, underwent differentiation that was sustained for at least 1 month. Some of the droplets produced by jetting contained only a few cells. These results indicate that the process of jetting does not significantly perturb neuronal cells and that this novel approach might in the future be a useful way to deposit small numbers of living nerve cells on to surfaces.
Eagles
Peter A M
PA
Kings College London, University of London, Randall Division of Cell and Molecular Biophysics, New Hunt's House, St. Thomas Street, London SE1 1UL, UK. peter.eagles@kcl.ac.uk
Qureshi
Amer N
AN
Jayasinghe
Suwan N
SN
D000818:Animals; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008954:Models, Biological; D009474:Neurons / Q000166:cytology*; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*