title: Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene.
abstract: BACKGROUND
  The expansion of specific trinucleotide repeats results in certain genetic disorders.
  
  
  METHODS
  Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)(n) alleles in the androgen receptor gene.
  
  
  RESULTS
  The difference in melting temperature (T(m)) between the most common normal and expanded alleles was approximately 1 degrees C.
  
  
  CONCLUSIONS
  Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.
authors.affiliation: Genetics Unit, Department of Obstetrics and Gynaecology, Ioannina University School of Medicine, Ioannina, Greece.
authors.forename: Anthoula
authors.initials: A
authors.lastname: Chatzikyriakidou
authors.forename: Christos
authors.initials: C
authors.lastname: Yapijakis
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Sofikitis
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Vassilopoulos
authors.forename: Ioannis
authors.initials: I
authors.lastname: Georgiou
mesh_terms: D000483:Alleles; D041321:Chromosomes, Human, X; D005260:Female; D006801:Humans; D008297:Male; D020966:Muscular Disorders, Atrophic / Q000235:genetics; D011944:Receptors, Androgen / Q000235:genetics*; D044366:Transition Temperature; D019680:Trinucleotide Repeat Expansion
chemical_list: C410798:AR protein, human; D011944:Receptors, Androgen