Compaction of single-chain DNA by histone-inspired nanoparticles.
We elaborated a versatile experimental model of chromatin which consists of a single chain of long duplex DNA that interacts with well-defined cationic nanoparticles of various sizes. We found that the DNA compaction by nanoparticles is stepwise and progressive at the single-chain level. It is controlled by the ability of DNA to wrap nanoparticles, which is more efficient for larger particles and, similar to DNA-histone interaction, is optimal at a physiological salt concentration.
Zinchenko
Anatoly A
AA
Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Yoshikawa
Kenichi
K
Baigl
Damien
D
D002843:Chromatin / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D008961:Models, Structural; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D010316:Particle Size
D002843:Chromatin; D006657:Histones; D004247:DNA