title: Precipitation of silver-thiosulfate complex and immobilization of silver by Cupriavidus metallidurans CH34.
abstract: Cupriavidus metallidurans CH34 is a facultative chemolithotrophic bacterium that possesses two megaplasmids (pMOL28 and pMOL30) that confer resistance to eleven metals. The ability of Cupriavidus metallidurans CH34 to resist silver is described here. Electronic microscopy, energy-dispersive X-ray (EDX) and X-ray diffractometry (DRX) observations revealed that C. metallidurans CH34 strongly associated silver with the outer membrane, under chloride chemical form. Using derivate strains of C. metallidurans CH34, which carried only one or no megaplasmid, we show that this resistance seems to be carried by pMOL30.
authors.affiliation: IUT Thionville-Yutz, Laboratoire d'Immunologie - Microbiologie (ESE-CNRS, UMR 7146), 1 Impasse Alfred Kastler, 57970, Yutz, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Ledrich
authors.forename: Sébastien
authors.initials: S
authors.lastname: Stemmler
authors.forename: Philippe
authors.initials: P
authors.lastname: Laval-Gilly
authors.forename: Laurent
authors.initials: L
authors.lastname: Foucaud
authors.forename: Jaïro
authors.initials: J
authors.lastname: Falla
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D020118:Cupriavidus necator / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D004351:Drug Resistance; D007700:Kinetics; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D013045:Species Specificity; D013885:Thiosulfates / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D013997:Time Factors; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D013885:Thiosulfates; D012834:Silver; C017717:sodium thiosulfate