Assessment of commercial test kits for identification of spring viraemia of carp virus.
Two test kits for the identification of spring viraemia of carp virus (SVCV), one an enzyme-linked immunosorbent assay (ELISA) using a rabbit polyclonal antiserum, and the other an indirect fluorescent antibody test (IFAT) using a mouse monoclonal antibody, were assessed for specificity using a range of virus isolates. The test viruses were selected from 4 recently described genogroups of piscine rhabdoviruses: Genogroup I (SVCV), Genogroup II (grass carp rhabdovirus), Genogroup III (pike fry rhabdovirus) and Genogroup IV ('tench rhabdovirus'). The test viruses included SVCV isolates from all 4 subgroups of Genogroup I. The ELISA was non-specific for these viruses and did not distinguish between SVCV and isolates from the other 3 Genogroups. However, the IFAT was too specific and detected SVCV isolates from only 1 of the 4 SVCV subgroups. Reliance on these test kits alone could result in misidentification of this OIE notifiable disease.
Dixon
P F
PF
CEFAS Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK. p.f.dixon@cefas.co.uk
Longshaw
C B
CB
D000818:Animals; D002347:Carps; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000175:diagnosis* / Q000821:virology*; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D013045:Species Specificity; D018116:Vesiculovirus / Q000302:isolation & purification*