Identification of nitrated proteins in Alzheimer's disease brain using a redox proteomics approach.
Nitric oxide (NO) has been implicated in the pathophysiology of a number of neurodegenerative diseases including Alzheimer's disease (AD). In the present study, using a proteomics approach, we identified enolase, glyceraldehyde-3-phosphate dehydrogenase, ATP synthase alpha chain, carbonic anhydrase-II, and voltage-dependent anion channel-protein as the targets of nitration in AD hippocampus, a region that shows a extensive deposition of amyloid beta-peptide, compared with the age-matched control brains. Immunoprecipitation and Western blotting techniques were used to validate the correct identification of these proteins. Our results are discussed in context of the role of oxidative stress as one of the important mechanisms of neurodegeneration in AD.
Sultana
Rukhsana
R
Department of Chemistry, University of Kentucky, Lexington, KY 40506, USA.
Poon
H Fai
HF
Cai
Jian
J
Pierce
William M
WM
Merchant
Michael
M
Klein
Jon B
JB
Markesbery
William R
WR
Butterfield
D Allan
DA
D025181:ATP Synthetase Complexes / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D024402:Carbonic Anhydrase II / Q000378:metabolism; D005260:Female; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D008297:Male; D009410:Nerve Degeneration / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods*; D014443:Tyrosine / Q000378:metabolism; D050995:Voltage-Dependent Anion Channel 1 / Q000378:metabolism
D009419:Nerve Tissue Proteins; D009566:Nitrates; C494762:VDAC1 protein, human; D014443:Tyrosine; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); D050995:Voltage-Dependent Anion Channel 1; D025181:ATP Synthetase Complexes; D024402:Carbonic Anhydrase II; D010751:Phosphopyruvate Hydratase