Stereological evaluation of the seminiferous tubules of rats after maternal undernutrition during the lactation period.
The goal of this study is to evaluate, through stereological methods, some structural aspects of offspring testes whose dams were submitted to protein and energy-restricted diets during the lactation period. At birth, dams were separated into 3 groups: control group (C), receiving a diet with 23% protein; protein-restricted group (PR), receiving a diet with 8% protein; energy-restricted group (ER), receiving a diet with 23% protein in restricted quantities. At weaning, the offspring was anesthetized and perfused with formalin solution. Then, the testes were excised and processed using routine histological methods. Compared to the C group, both PR and ER groups had a significant reduction in the testis weight (PR = 65%, ER = 60%, p < 0.01), in the total area (PR = 23%, ER = 32%, p < 0.01), in the luminal area (PR = 30%, ER = 36%, p < 0.01), in the epithelial area (PR = 21%, ER = 27%, p < 0.01), and in the epithelial height (PR = 17%, ER = 23%, p < 0.01) of the seminiferous tubule. We conclude that maternal malnutrition during lactation leads to structural changes in the testis that could be responsible for future alterations in this organ physiology.
Ramos
Cristiane da Fonte
Cda F
Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil. cramos_uerj@yahoo.com.br
da Silva
Alexandra Moreira
AM
Costa
Waldemar Silva
WS
Sampaio
Francisco J B
FJ
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000831:Animals, Newborn; D007774:Lactation; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D009929:Organ Size; D011502:Protein-Energy Malnutrition; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000473:pathology*; D013737:Testis / Q000473:pathology