title: The effect of in vitro recombinant factor VIIA on coagulation parameters for blood taken during liver transplantation.
abstract: Recombinant factor VIIa (rFVIIa) has been utilized in pilot studies in orthotopic liver transplantation (OLT) when administered to patients at doses of 68.37 microg/kg and 80 microg/kg. Although some effectiveness in normalizing measurements of coagulation has been demonstrated, the optimal dose for patients undergoing OLT has not been established. This study evaluated the effects of an in vitro equivalent dose of 120 microg/kg of rFVIIa on coagulation parameters when applied to the blood drawn from patients undergoing OLT. Coagulation function was assessed in 10 patients at four points during OLT. These time points were baseline, 5 minutes prior to reperfusion, 10 minutes after reperfusion, and 70 minutes after reperfusion. These patients did not receive rFVIIa perioperatively. At each of these four time points, a native sample was analyzed for prothrombin time (PT) and thromboelastogram. The rFVIIa (6.1 microg/kg or the approximate equivalent dose of 120 microg/kg for a 70 kg patient) was added to a second sample from the same patient. This second sample was also analyzed for PT and thromboelastogram. There was a statistically significant difference in baseline PT between native versus rFVIIa supplemented samples (15.8 +/- 3.21 vs 13.6 +/- 2.36 seconds, P < .02). The maximum amplitude of the thromboelastogram was larger in the native samples at 5 minutes prior to reperfusion (53.5 mm vs 39 mm, P < .02). No significant differences existed in the variables at any of the other sampling times. This study failed to demonstrate a consistent in vitro effect of rFVIIa on the blood taken from patients during OLT.
authors.affiliation: Anesthesiology Department, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA. gali.bhargavi@mayo.edu
authors.forename: B
authors.initials: B
authors.lastname: Gali
authors.forename: S R
authors.initials: SR
authors.lastname: Rettke
authors.forename: D J
authors.initials: DJ
authors.lastname: Plevak
authors.forename: G A
authors.initials: GA
authors.lastname: Nuttall
authors.forename: P J
authors.initials: PJ
authors.lastname: Santrach
authors.forename: D R
authors.initials: DR
authors.lastname: Schroeder
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects / Q000502:physiology*; D001803:Blood Transfusion; D005169:Factor VIII / Q000627:therapeutic use*; D006801:Humans; D017114:Liver Failure, Acute / Q000601:surgery; D016031:Liver Transplantation / Q000379:methods*; D016343:Monitoring, Intraoperative; D011994:Recombinant Proteins / Q000627:therapeutic use
chemical_list: D011994:Recombinant Proteins; D005169:Factor VIII