Molecular taxonomy and ecology of Pseudallescheria, Petriella and Scedosporium prolificans (Microascaceae) containing opportunistic agents on humans.
The main purpose of the present paper is to establish the connection between phylogenetic and morphological data and ecological features of strains of Pseudallescheria, Petriella, and Scedosporium. For the phylogenetic analysis sequences of the ITS region and the large subunit (partial sequences) of the rDNA were used. Cultural characteristics were observed on MEA 2 % and Weitzman-Silva Hutner Agar. Results showed, that three major groups could be differentiated, corresponding to Pseudallescheria, Petriella and S. prolificans. Among Petriella species only Pe. setifera is reasonably delimited. Pe. musispora was found to be synonymous with Pe. setifera. S. prolificans proved to be a homogenous species on the basis of ITS-sequences. Morphologically, Pseudallescheria and Petriella are distinguished by ostiolate vs non-ostiolate ascomata, a bipartition reflected also in ITS sequence data. We hypothesise a secondary loss of the ostiole of Pseudallescheria due to its ecological preferences. Infraspecific grouping within the highly variable species P. boydii is consistent for at least one clade in the ITS tree. The evolution of lineages with increased virulence within P. boydii is discussed.
Rainer
Johannes
J
Institute of Microbiology, Leopold Franzens University, Technikerstrasse 25, A-6020 Innsbruck, Austria. j.rainer@uibk.ac.at
De Hoog
G Sybren
GS
D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D017753:Ecosystem; D006801:Humans; D008969:Molecular Sequence Data; D009894:Opportunistic Infections / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011541:Pseudallescheria / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021681:Scedosporium / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D016415:Sequence Alignment
D004271:DNA, Fungal; D021901:DNA, Intergenic; D012335:RNA, Ribosomal