title: Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2.
abstract: Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) are two major inflammatory mediators. Here we show that iNOS specifically binds to COX-2 and S-nitrosylates it, enhancing COX-2 catalytic activity. Selectively disrupting iNOS-COX-2 binding prevented NO-mediated activation of COX-2. This synergistic molecular interaction between two inflammatory systems may inform the development of anti-inflammatory drugs.
authors.affiliation: Department of Neuroscience, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.
authors.forename: Sangwon F
authors.initials: SF
authors.lastname: Kim
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Huri
authors.forename: Solomon H
authors.initials: SH
authors.lastname: Snyder
mesh_terms: D000818:Animals; D001710:Biotin / Q000378:metabolism; D002384:Catalysis; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D051379:Mice; D020030:Nitric Oxide Donors / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D009603:Nitroso Compounds / Q000378:metabolism*; D011485:Protein Binding; D026422:S-Nitrosoglutathione / Q000378:metabolism
chemical_list: D020030:Nitric Oxide Donors; D009603:Nitroso Compounds; D026422:S-Nitrosoglutathione; D001710:Biotin; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D003545:Cysteine