Genetic diversity within a caste population of India as measured by Y-chromosome haplogroups and haplotypes: subcastes of the Golla of Andhra Pradesh.
The extent of population subdivision based on 15 Y-chromosome polymorphisms was studied in seven subcastes of the Golla (Karnam, Pokanati, Erra, Doddi, Punugu, Puja, and Kurava), who inhabit the Chittoor district of southern Andhra Pradesh, India. These Golla subcastes are traditionally pastoralists, culturally homogeneous and endogamous. DNA samples from 146 Golla males were scored for seven unique event polymorphisms (UEPs) and eight microsatellites, permitting allocation of each into haplogroups and haplotypes, respectively. Genetic diversity (D) was high (range, 0.9048-0.9921), and most of the genetic variance (>91%) was explained by intrapopulation differences. Median-joining network analysis of microsatellite haplotypes demonstrated an absence of any structure according to subcaste affiliation. Superimposition of UEPs on this phylogeny, however, did create some distinct clusters, indicating congruence between haplotype and haplogroup phylogenies. Our results suggest many male ancestors for the Golla as well as for each of the subcastes. Genetic distances among the seven subcastes, based on autosomal markers (short tandem repeats and human leukocyte antigens) as well as those on the chromosome Y, indicate that the Kurava may not be a true subcaste of the Golla. Although this finding is based on a very small Kurava sample, it is in accordance with ethnohistorical accounts related by community elders. The Punugu was the first to hive off the main Golla group, and the most recently separated subcastes (Karnam, Erra, Doddi, and Pokanati) fissioned from the Puja. This phylogeny receives support from the analysis of autosomal microsatellites as well as HLA loci in the same samples. In particular, there is a significant correlation (r = 0.8569; P = 0.0097) between Y-chromosome- and autosomal STR-based distances.
Mitchell
R John
RJ
Department of Genetics and Human Variation, School of Molecular Sciences, La Trobe University, Melbourne, Victoria 3086, Australia. john.mitchell@latrobe.edu.au
Reddy
B Mohan
BM
Campo
David
D
Infantino
Tania
T
Kaps
Maria
M
Crawford
Michael H
MH
D000704:Analysis of Variance; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D014644:Genetic Variation; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D007194:India; D008297:Male; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D012923:Social Class