[Regulation of expression of pituitary tumor transforming gene 1 (PTTG1) by androgen in prostate cancer].
OBJECTIVE
To identify the androgen-responsive genes in prostate and screen the molecular targets for further studying human prostate cancer.


METHODS
The potential androgen-responsive gene pituitary tumor transforming gene 1 (PTTG1) was selected which had been previously screened by cDNA microarray in rat prostate and its mRNA level was detected by Northern blot in the castrated rat prostate with and without replacement of Mibolerone. Immunohistochemistry was performed to determine the expression and location of PTTG1 in human prostate tissues. Then human androgen-dependent prostate cancer cells LNCaP were used as a model to study the regulation of PTTG1 by Mibolerone.


RESULTS
PTTG1 mRNA was hardly detectable in the prostate of 7-day castrated rats, while it was up-regulated dramatically in the prostate of 7-day castrated rats treated with Mibolerone for 2 days. It was showed that high expression of PTTG1 was localized to the epithelial cells of human prostate cancer but not to the stromal cells with Immunohistochemistry. Northern blot analysis indicated that LNCaP cells treated with 0.1 nmol/L Mibolerone for 2 days led to the high PTTG1 mRNA expression. The basic expression of PTTG1 in human androgen-independent prostate cancer cell lines PC3 or DU145 was even higher than that in the human androgen-dependent prostate cancer cells LNCaP treated with Mibolerone.


CONCLUSIONS
Androgen can up-regulate the PTTG1 expression in castrated rat prostate and human prostate cancer cell LNCaP. It suggests that PTTG1 is potential to play an important role in human prostate cancer progression.
Xin
Dian-qi
DQ
Department of Urology, Peking University First Hospital; Institute of Urology, Peking University, Beijing 100034, China.
Zhu
Xu-hui
XH
Lai
Yong-qing
YQ
You
Ran
R
Na
Yan-qun
YQ
Guo
Ying-lu
YL
Mao
Ze-bin
ZB
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000818:Animals; D015152:Blotting, Northern; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009919:Orchiectomy; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D064249:Securin; D045165:Testosterone Congeners / Q000494:pharmacology
D009363:Neoplasm Proteins; D012333:RNA, Messenger; D064249:Securin; D045165:Testosterone Congeners; C578002:pituitary tumor-transforming protein 1, human; D009277:Nandrolone; C100075:mibolerone