Effect of antispermatogenic agents on cell marker enzymes of rat Sertoli cells in vitro.
OBJECTIVE
To examine the role of Sertoli cells in the antispermatogenic action of two nonsteroidal male contraceptive compounds (CDRI-84/35 and gossypol) by evaluating their effect on some key parameters of Sertoli cell function in vitro.


METHODS
Primary cultures of Sertoli cell were established from 18-day-old rat testis and treated on day 5 with different concentrations (1.0, 0.1, 0.01, and 0.001 mM) of either CDRI-84/35 or gossypol in vitro. Lactate (secretion), along with beta-glucuronidase, gamma-glutamyl transpeptidase, lactate dehydrogenase (LDH) and aromatase activities, was measured in these cells to examine the functions targeted by antispermatogenic agents in Sertoli cells.


RESULTS
CDRI-84/35 significantly affected Sertoli cell parameters (stimulation in most of the cases) that are important for germ cell development like lactate secretion, LDH activity, aromatase activity (estradiol secretion) and so on. Gossypol in comparison to CDRI-84/35 had a more severe effect on Sertoli cells with complete inhibition of enzyme activities at higher concentrations.


CONCLUSIONS
It is probable that the antispermatogenic action of CDRI-84/35 and gossypol is routed through Sertoli cells by disruption of important cell functions that support spermatogenesis in vivo. However, the two compounds appear to have different course of action in Sertoli cells, ultimately leading to spermatogenic failure.
Ojha
Priti
P
Division of Endocrinology, Central Drug Research Institute, Lucknow-226 001, India.
Dhar
Janak Dulari
JD
Dwivedi
Anil Kumar
AK
Singh
Ram Lakhan
RL
Gupta
Gopal
G
D000818:Animals; D000988:Antispermatogenic Agents / Q000494:pharmacology*; D001141:Aromatase / Q000378:metabolism; D002478:Cells, Cultured; D005966:Glucuronidase / Q000378:metabolism; D006072:Gossypol / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000187:drug effects* / Q000201:enzymology*; D005723:gamma-Glutamyltransferase / Q000378:metabolism
C111137:1-formyl-4-dichloroacetylpiperazine; D000988:Antispermatogenic Agents; D010879:Piperazines; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D001141:Aromatase; D005723:gamma-Glutamyltransferase; D005966:Glucuronidase; D006072:Gossypol