Optimization of growth for the hyperthermophilic archaeon Aeropyrum pernix on a small-batch scale.
Growth of Aeropyrum pernix, the first reported aerobic neutrophilic hyperthermophilic archaeon, was investigated under different cultivation parameters. Different sources of seawater, pH, and the cultivation methods were tested with the aim to improve the biomass production. A 1-L glass flask fitted with a condenser and air diffuser was used as a bioreactor. The optimum conditions for maximizing A. pernix biomass were obtained when Na2S2O3.5H2O (1 g/L) with added marine broth 2216 at pH 7.0 (20 mmol HEPES buffer/L) was used as a growing medium in a 1-L flask. The biomass production was 0.45 g dry cell mass/L in 40 h under the optimum conditions, which is more than the 0.42 g dry cell mass/L in 60 h previously obtained.
Milek
Igor
I
Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Slovenia.
Cigic
Blaz
B
Skrt
Mihaela
M
Kaletunç
Gönül
G
Ulrih
Natasa Poklar
NP
D041522:Aeropyrum / Q000254:growth & development*; D018533:Biomass; D003470:Culture Media; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008828:Microbiological Techniques / Q000295:instrumentation / Q000379:methods; D012623:Seawater; D013696:Temperature
D003470:Culture Media