title: Identification of basogranulin (BB1) as a novel immunohistochemical marker of basophils in normal bone marrow and patients with myeloproliferative disorders.
abstract: In myeloproliferative disorders (MPDs), basophils typically increase in number in the bone marrow (BM) and blood. In chronic myeloid leukemia (CML), basophilia is a diagnostic and prognostic variable. However, no reliable approach for routine detection and enumeration of basophils in BM sections is available. We applied the antibasogranulin antibody BB1 on paraffin-embedded BM sections in 21 control samples (normal BM), 45 patients with CML, 9 with chronic idiopathic myelofibrosis, 11 with polycythemia vera, 19 with essential thrombocythemia, and 7 with indolent systemic mastocytosis. As assessed by immunostaining of serial BM sections, BB1+ cells coexpressed myeloperoxidase, histidine decarboxylase, and leukosialin but did not express B- or T-cell-restricted antigens. BB1+ BM cells were found to be highly elevated in patients with CML compared with normal BM or other MPDs, with maximum counts found in accelerated phase CML (median, 160 cells/mm(2)). In summary, BB1 (basogranulin) is a new immunohistochemical basophil marker that should allow quantification of basophils in CML at diagnosis and during therapy.
authors.affiliation: Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.
authors.forename: Hermine
authors.initials: H
authors.lastname: Agis
authors.forename: Maria-Theresa
authors.initials: MT
authors.lastname: Krauth
authors.forename: Alexandra
authors.initials: A
authors.lastname: Böhm
authors.forename: Isabella
authors.initials: I
authors.lastname: Mosberger
authors.forename: Leonhard
authors.initials: L
authors.lastname: Müllauer
authors.forename: Ingrid
authors.initials: I
authors.lastname: Simonitsch-Klupp
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Walls
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Horny
authors.forename: Peter
authors.initials: P
authors.lastname: Valent
mesh_terms: D000328:Adult; D001491:Basophils / Q000737:chemistry*; D015415:Biomarkers; D001853:Bone Marrow / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000032:analysis*; D014157:Transcription Factors / Q000032:analysis*
chemical_list: D015415:Biomarkers; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D014157:Transcription Factors; C077914:BNC1 protein, human