Simulated moving bed technology with a simplified approach for protein purification. Separation of lactoperoxidase and lactoferrin from whey protein concentrate.
Simulated moving bed (SMB) technology is a continuous chromatographic technique proven to have many advantages compared to conventional batch chromatography, such as: raised productivity and product concentration, reduced buffer consumption as well as more efficient use of raw material. In this study a 20 column SMB process for the separation of lactoperoxidase and lactoferrin from whey protein concentrate (WPC) was developed. A simplified approach with data from a single column experiment was used when designing the process. The SMB process data were compared to a theoretical scale-up of the breakthrough experiment reflecting the same 20 column set-up run in non-moving bed mode. The outcome of the comparison is a 48% raise in productivity, a 4.3 times decrease in buffer consumption, 6.5 times raise in target protein concentration with a raw material utilization which is slightly better for the SMB process.
Andersson
Jonatan
J
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-22100 Lund, Sweden.
Mattiasson
Bo
B
D002852:Chromatography, Ion Exchange / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D007781:Lactoferrin / Q000302:isolation & purification*; D007784:Lactoperoxidase / Q000302:isolation & purification*; D008894:Milk Proteins / Q000737:chemistry*; D000067816:Whey Proteins
D008894:Milk Proteins; D000067816:Whey Proteins; D007784:Lactoperoxidase; D007781:Lactoferrin