title: [Study on the reaction of proteinase from Bacillus subtilis with alizarin complex].
abstract: The interaction of bacillus subtilis proteinase(BSP) and Alizarin Complex (ALC) was investigated by spectrophotometric method in acidic solution(pH 4.20). When BSP was added into ALC, a red color was observed, which indicated the formation of BSP-ALC associated compound. The maximum absorption of the red color coordination compound was obtained at 510 nm. It was found that the red shift of maximum absorption of the complex was 85 nm. According to balance percolation method, molar ratio method and double wavelengh method, the apparent molar absorptivity epsilonB = 6.68 x 10(3) L x mol(-1) x cm(-1). Conditional constants were defined, the maximum binding number n = 10, and the apparent binding constant K = 7. 25 x 10(6) L x mol(-1). It was found that Scatchard model is appropriate in the treatment of data obtained here.
authors.affiliation: Department of Chemistry and Biology Engineering, Southwest University of Science and Technology, Mianyang 621010, China.
authors.forename: Xing-ming
authors.initials: XM
authors.lastname: Wang
authors.forename: Fa-qing
authors.initials: FQ
authors.lastname: Dong
authors.forename: Li-sheng
authors.initials: LS
authors.lastname: Ding
authors.forename: Ding-ming
authors.initials: DM
authors.lastname: Yang
authors.forename: Rong-min
authors.initials: RM
authors.lastname: Shi
mesh_terms: D000465:Algorithms; D000880:Anthraquinones / Q000737:chemistry* / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D008956:Models, Chemical; D015394:Molecular Structure; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013053:Spectrophotometry
chemical_list: D000880:Anthraquinones; D001426:Bacterial Proteins; C010078:alizarin; D010447:Peptide Hydrolases