title: EPR monitoring of the bioavailability of an organic xenobiotic (4-hydroxy-TEMPO) in model clay suspensions and pastes.
abstract: Electron paramagnetic resonance spectroscopy is used to monitor the bioavailability of a nitroxide spin probe, 4-hydroxy-Tempo or Tempol, in Ca-hectorite suspensions and pastes, to bacteria capable of degrading this probe co-metabolically. In nutrient solutions with an initial probe concentration of 1.2 mM and in the absence of hectorite, bacteria are able to denature Tempol and eliminate its paramagnetic signal within 48 h. In the presence of hectorite and after flocculation, the effect of bacteria is significantly delayed, but almost complete denaturation still occurs, after roughly 120 h. When hectorite is added but the bacterial/clay suspension is not centrifuged, Tempol denaturation levels off after about 24 h and reaches a plateau with approximately 45% of Tempol remaining. This plateau does not constitute evidence of limited bioavailability, as is widely assumed, since subsequent addition of nutrients causes the denaturation reaction to proceed to a second plateau, with merely 10% of Tempol remaining.
authors.affiliation: Laboratory of Geoenvironmental Science and Engineering, Bradfield Hall, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Alain
authors.initials: A
authors.lastname: Dumestre
authors.forename: Matteo
authors.initials: M
authors.lastname: Spagnuolo
authors.forename: Rebecca
authors.initials: R
authors.lastname: Bladon
authors.forename: Jacques
authors.initials: J
authors.lastname: Berthelin
authors.forename: Philippe
authors.initials: P
authors.lastname: Baveye
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry*; D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D001682:Biological Availability; D000077215:Clay; D003497:Cyclic N-Oxides / Q000032:analysis* / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004784:Environmental Monitoring / Q000379:methods*; D017640:Silicates / Q000494:pharmacology; D012989:Soil Pollutants / Q000032:analysis*; D013113:Spin Labels; D015262:Xenobiotics / Q000032:analysis* / Q000378:metabolism
chemical_list: D000538:Aluminum Silicates; D003497:Cyclic N-Oxides; D017640:Silicates; D012989:Soil Pollutants; D013113:Spin Labels; D015262:Xenobiotics; C013547:hectorite; D000077215:Clay; C001803:tempol