Diagnostic oligonucleotide microarray fingerprinting of Bacillus isolates.
A genome-independent microarray and new statistical techniques were used to genotype Bacillus strains and quantitatively compare DNA fingerprints with the known taxonomy of the genus. A synthetic DNA standard was used to understand process level variability and lead to recommended standard operating procedures for microbial forensics and clinical diagnostics.
Chandler
Darrell P
DP
Argonne National Laboratory, IL 60439, USA. dchandler@anl.gov
Alferov
Oleg
O
Chernov
Boris
B
Daly
Don S
DS
Golova
Julia
J
Perov
Alexander
A
Protic
Miroslava
M
Robison
Richard
R
Schipma
Matthew
M
White
Amanda
A
Willse
Alan
A
D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods; D016680:Genome, Bacterial; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010802:Phylogeny