title: GLR1 plays an essential role in the homeodynamics of glutathione and the regulation of H2S production during respiratory oscillation of Saccharomyces cerevisiae.
abstract: The role of glutathione (GSH) and its homeodynamics during respiratory oscillation of Saccharomyces cerevisiae were investigated. Pulse injection of thiol redox modifying agents, such as diethylmaleate, N-ethylmaleimide, DL-butione-[S,R]-sulfoxamine, or 5-nitro-2-furaldehyde into the culture perturbed oscillation, although the degree of perturbation varied. Analysis of the expression profiles of GSH1 and GLR1, the activities of glutathione reductase, oscillations in cysteine and GSH concentrations, and the chemostat culture of the GLR1 disruptant indicated that GLR1 plays an essential role in the homeodynamics of GSH and the regulation of H(2)S production.
authors.affiliation: Department of Food and Nutrition, Andong National University, Korea. hysohn@andong.ac.kr
authors.forename: Ho-Yong
authors.initials: HY
authors.lastname: Sohn
authors.forename: Eun-Joo
authors.initials: EJ
authors.lastname: Kum
authors.forename: Gi-Seok
authors.initials: GS
authors.lastname: Kwon
authors.forename: Ingnyol
authors.initials: I
authors.lastname: Jin
authors.forename: Claire A
authors.initials: CA
authors.lastname: Adams
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kuriyama
mesh_terms: D017931:DNA Primers; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000502:physiology; D005978:Glutathione / Q000378:metabolism*; D006862:Hydrogen Sulfide / Q000378:metabolism*; D010084:Oxidation-Reduction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; C508746:GLR1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C490862:GSH1 protein, S cerevisiae; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione; D006862:Hydrogen Sulfide