Cloning and sequencing of the aldehyde oxidase gene from Methylobacillus sp. KY4400.
The aldehyde oxidase genes (aods) from Methylobacillus sp. KY4400 were cloned, and sequenced. The sequences for small (aodS, 489 bp), medium (aodM, 993 bp), and large (aodL, 2,328 bp) subunit genes were determined. At least one additional ORF was indispensable for the expression of enzyme activity. The structural genes contained two [2Fe-2S] centers, an FAD binding site, and a molybdenum cofactor binding site.
Yasuhara
Akinori
A
BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd., Tokyo, Japan.
Akiba-Goto
Miho
M
Aisaka
Kazuo
K
D042931:Aldehyde Oxidase / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D030541:Databases, Genetic; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D020631:Methylobacillus / Q000201:enzymology* / Q000235:genetics*; D008970:Molecular Weight; D008982:Molybdenum / Q000378:metabolism; D020539:Sequence Analysis, Protein; D014969:Xanthine Oxidase / Q000737:chemistry / Q000235:genetics
D004269:DNA, Bacterial; D005182:Flavin-Adenine Dinucleotide; D008982:Molybdenum; D014969:Xanthine Oxidase; D042931:Aldehyde Oxidase