title: Quorum-sensing signal synthesis by the Yersinia pestis acyl-homoserine lactone synthase YspI.
abstract: The acyl-homoserine lactone molecular species (AHLs) produced by the Yersinia pestis AHL synthase YspI were identified by biochemical and physical/chemical techniques. Bioassays of extracts from culture supernatants of the recombinant YspI and wild-type Yersinia pestis showed similar profiles of AHLs. Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-L-homoserine lactone and N-3-oxo-hexanoyl-L-homoserine lactone.
authors.affiliation: Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, P.O. Box 8511 MS8303, Aurora, CO 80045, USA.
authors.forename: J Paul
authors.initials: JP
authors.lastname: Kirwan
authors.forename: Ty A
authors.initials: TA
authors.lastname: Gould
authors.forename: Herbert P
authors.initials: HP
authors.lastname: Schweizer
authors.forename: Scott W
authors.initials: SW
authors.lastname: Bearden
authors.forename: Robert C
authors.initials: RC
authors.lastname: Murphy
authors.forename: Mair E A
authors.initials: ME
authors.lastname: Churchill
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D015010:Yersinia pestis / Q000201:enzymology / Q000502:physiology*
chemical_list: D011994:Recombinant Proteins; C088386:homoserine lactone; C030654:N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone; D008025:Ligases; C449816:N-acylhomoserine lactone synthase; D015107:4-Butyrolactone