fgf20 is essential for initiating zebrafish fin regeneration.
Epimorphic regeneration requires the presence or creation of pluripotent cells capable of reproducing lost organs. Zebrafish fin regeneration is mediated by the creation of blastema cells. Here, we characterize the devoid of blastema (dob) mutant that fails fin regeneration during initial steps, forms abnormal regeneration epithelium, and does not form blastema. This mutation has no impact on embryonic survival. Dob results from an fgf20a null mutation, Y148S. Fgf20a is expressed during initiation of fin regeneration at the epithelial-mesenchymal boundary and later overlaps with the blastema marker msxb. Thus, fgf20a has a regeneration-specific requirement, initiating fin regeneration, and controlling blastema formation.
Whitehead
Geoffrey G
GG
Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Department of Cardiology, Children's Hospital, Boston, MA 02115, USA.
Makino
Shinji
S
Lien
Ching-Ling
CL
Keating
Mark T
MT
D000818:Animals; D005121:Extremities; D005346:Fibroblast Growth Factors / Q000502:physiology*; D018398:Homeodomain Proteins / Q000096:biosynthesis; D008297:Male; D008648:Mesoderm; D009154:Mutation; D012038:Regeneration / Q000235:genetics / Q000502:physiology*; D013696:Temperature; D014945:Wound Healing; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000096:biosynthesis / Q000502:physiology*
D018398:Homeodomain Proteins; D029961:Zebrafish Proteins; C503561:fibroblast growth factor 20, zebrafish; C460082:msx1b protein, zebrafish; D005346:Fibroblast Growth Factors