title: Cadmium toxicity related to cysteine metabolism and glutathione levels in frog Rana ridibunda tissues.
abstract: The level of glutathione and sulfane sulfur and sulfurtransferases activity in adult frogs Rana ridibunda were investigated after the exposure to 40 mg or 80 mg CdCl(2) L(-1) for 96 h or 240 h. Cd accumulation in the liver, kidneys and testes was confirmed, and the highest Cd level was found in the testes. In the liver, the exposure to Cd resulted in an increase of GSH level and the activity of rhodanese, while the activity of 3-mercaptopyruvate sulfurtransferase and cystathionase decreased. The kidneys and brain showed the elevated level of GSH and the activity of all investigated sulfurtransferases, as well as sulfane sulfur especially in brain. In such tissues as the testes, muscles and heart, the level of GSH and the activity of 3-mercaptopyruvate sulfurtransferase were significantly diminished. The increased level of sulfane sulfur was determined in the testes and muscles and the increased activity of rhodanese in the testes and the heart. These findings suggest the possible role of sulfane sulfur and/or sulfurtransferases in the antioxidation processes, which can be generated in cells by cadmium.
authors.affiliation: Department of Human Developmental Biology, Collegium Medicum, Jagiellonian University, Kraków, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Sura
authors.forename: Natalia
authors.initials: N
authors.lastname: Ristic
authors.forename: Patrycja
authors.initials: P
authors.lastname: Bronowicka
authors.forename: Maria
authors.initials: M
authors.lastname: Wróbel
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D003542:Cystathionine gamma-Lyase / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism*; D004220:Disulfides / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011895:Rana ridibunda / Q000378:metabolism*; D013466:Sulfurtransferases / Q000378:metabolism*; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D013884:Thiosulfate Sulfurtransferase / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D004220:Disulfides; D002104:Cadmium; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase; C021550:3-mercaptopyruvate sulphurtransferase; D003542:Cystathionine gamma-Lyase; D005978:Glutathione; D003545:Cysteine