Pentoxifylline inhibits mature burn scar fibroblasts in culture.
Fibroblasts are thought to be (in part) responsible for the persisting contractile forces that result in burn contractures. Using monolayer and fibroblast populated collagen lattice (FPCL) models we subjected burn scar fibroblasts to the anti-fibrinolytic agent Pentoxifylline (PFX) in an attempt to reduce proliferation and contraction of these cells. Fibroblasts were isolated from mature burn scars at reconstructive surgery. Fibroblasts were grown in monolayer or incorporated into FPCL's and exposed to PFX. Fibroblast numbers and FPCL surface areas were calculated using digital photography and image analysis. PFX showed a dose-dependent inhibition of contraction and reduced proliferation of burn scar fibroblasts. In monolayer, cell number proliferation was markedly reduced. FPCL's containing 0, 0.25, 0.5, 1, and 2 mg/ml of PFX had relative surface areas of 31, 40, 43, 59, and 85%, respectively. One and 2 mg/ml FPCL's contracted significantly less than controls (p < 0.0001). This is the first study to show the dose-dependent effects of Pentoxifylline on the proliferation and contraction of burn scar fibroblasts. This study suggests that Pentoxifylline has a direct effect on inhibiting burn scar fibroblasts. Further study of PFX on burn scars will provide opportunities to reduce burn scar contractures in vivo.
Rawlins
J M
JM
Plastic Surgery Department, Castle Hill Hospital, Cottingham, Hull HU16 5JQ, UK. J.M.Rawlins@Bradford.ac.uk
Lam
W L
WL
Karoo
R O
RO
Naylor
I L
IL
Sharpe
D T
DT
D002056:Burns / Q000150:complications*; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000517:prevention & control*; D003286:Contracture / Q000209:etiology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D006401:Hematologic Agents / Q000008:administration & dosage*; D006801:Humans; D010431:Pentoxifylline / Q000008:administration & dosage*; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects
D006401:Hematologic Agents; D010431:Pentoxifylline