title: High level expression of Prop-1 gene in gonadotropic cell lines.
abstract: Prop-1 acts as an upstream regulator for the Pit-1 gene to induce development of Pit-1 lineage pituitary cell lines, GH-, PRL-, and TSH-producing cells, in the early stage of pituitary organogenesis. Furthermore, Prop-1 is presumed to be involved in the function of FSH/LH-producing cells, gonadotropes, since the defective Prop-1 gene shows hypogonadism. Recently, we reported evidence that Prop-1 directly regulates expression of the porcine FSHbeta gene, thus providing a novel advance in understanding the function of Prop-1 in FSH/LH production and hypogonadism. This study was intended to demonstrate the expressions of Prop-1 gene in pituitary tumor-derived cell lines. RT-PCR analyses were conducted of Pit-1, glycoprotein alpha subunit (alphaGSU), GnRH receptor, and cyclophilin A (a ubiquitously expressing gene). We observed expression of the Pit-1 gene in alphaT1-1, TalphaT1, MtT/S, GH3, and TtT/GF cells, expression of the alphaGSU gene in alphaT1-1, alphaT3-1, LbetaT2, LbetaT4, TalphaT1, and GH3 cells, and expression of GnRH receptor gene in alphaT3-1, LbetaT2, LbetaT4, and GH3 cells, respectively. These expression profiles were in accord with their cell lineages, with only a few exceptions. To accurately measure the expression level of the Prop-1 gene, a quantitative analysis was performed using the real-time PCR method. This analysis demonstrated that the LbetaT2 and LbetaT4 gonadotrope cell lines, which express the FSHbeta gene, express the Prop-1 gene. Taken together with our previous observation that Prop-1 is present in the adult porcine pituitary gonadotropes, Prop-1 might also be involved in development of gonadotropes and hormone production.
authors.affiliation: Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University, Kawasaki, Kanagawa, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Aikawa
authors.forename: Takanobu
authors.initials: T
authors.lastname: Sato
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Ono
authors.forename: Takako
authors.initials: T
authors.lastname: Kato
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D019070:Cell Lineage; D006224:Cricetinae; D021984:Cyclophilin A / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D037201:Follicle Stimulating Hormone, beta Subunit / Q000378:metabolism; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000378:metabolism; D006062:Gonadotropins / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D006728:Hormones / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D010902:Pituitary Gland / Q000166:cytology*; D010911:Pituitary Neoplasms / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D037201:Follicle Stimulating Hormone, beta Subunit; D006023:Glycoproteins; D006062:Gonadotropins; D018398:Homeodomain Proteins; D006728:Hormones; C102134:Prophet of Pit-1 protein; D012313:RNA; D021984:Cyclophilin A