Restriction of foamy viruses by APOBEC cytidine deaminases.
Foamy viruses (FVs) are nonpathogenic retroviruses infecting many species of mammals, notably primates, cattle, and cats. We have examined whether members of the apolipoprotein B-editing catalytic polypeptide-like subunit (APOBEC) family of antiviral cytidine deaminases restrict replication of simian FV. We show that human APOBEC3G is a potent inhibitor of FV infectivity in cell culture experiments. This antiviral activity is associated with cytidine editing of the viral genome. Both molecular FV clones and primary uncloned viruses were susceptible to APOBEC3G, and viral infectivity was also inhibited by murine and simian APOBEC3G homologues, as well as by human APOBEC3F. Wild-type and bet-deleted viruses were similarly sensitive to this antiviral activity, suggesting that Bet does not significantly counteract APOBEC proteins. Moreover, we did not detect FV sequences that may have been targeted by APOBEC in naturally infected macaques, but we observed a few G-to-A substitutions in humans that have been accidentally contaminated by simian FV. In infected hosts, the persistence strategy employed by FV might be based on low levels of replication, as well as avoidance of cells expressing large amounts of active cytidine deaminases.
Delebecque
Frédéric
F
Virus and Immunity Group, URA CNRS 1930, Institut Pasteur, 75724 Paris cedex 15, France.
Suspène
Rodolphe
R
Calattini
Sara
S
Casartelli
Nicoletta
N
Saïb
Ali
A
Froment
Alain
A
Wain-Hobson
Simon
S
Gessain
Antoine
A
Vartanian
Jean-Pierre
JP
Schwartz
Olivier
O
D000071480:APOBEC-3G Deaminase; D000225:Adenine; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003564:Cytidine Deaminase / Q000737:chemistry / Q000378:metabolism*; D016679:Genome, Viral / Q000235:genetics; D006147:Guanine; D006801:Humans; D008251:Macaca; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009700:Nucleoside Deaminases / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D013045:Species Specificity; D016092:Spumavirus / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
D012097:Repressor Proteins; D006147:Guanine; D009700:Nucleoside Deaminases; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase; D000225:Adenine