title: Anucleate cell blue assay: a useful tool for identifying novel type II topoisomerase inhibitors.
abstract: About 95,000 compounds were screened by the anucleate cell blue assay. Fifty-one of the hit compounds had various structures and showed inhibitory activity against DNA gyrase and/or topoisomerase IV. Moreover, the compounds exhibited antibacterial activity against a fluoroquinolone- and novobiocin-resistant strain of Staphylococcus aureus. The anucleate cell blue assay is therefore a useful tool for finding novel type II topoisomerase inhibitors.
authors.affiliation: Pharmacology & Microbiology Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Enoki 33-94, Suita, Osaka 564-0053, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Oyamada
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ito
authors.forename: Mika
authors.initials: M
authors.lastname: Fujimoto-Nakamura
authors.forename: Akihiko
authors.initials: A
authors.lastname: Tanitame
authors.forename: Noritaka
authors.initials: N
authors.lastname: Iwai
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nagai
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Yamagishi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Wachi
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000201:enzymology*; D001681:Biological Assay / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D024841:Fluoroquinolones; D059005:Topoisomerase II Inhibitors
chemical_list: D004396:Coloring Agents; D004791:Enzyme Inhibitors; D024841:Fluoroquinolones; D059005:Topoisomerase II Inhibitors