[Experimental research on immunological rejection in neural stem cells allograft].
OBJECTIVE
To investigate the occurrence of immunological rejection in brain transplantation of neural stem cells (NSCs) in the rat of Parkinson's disease model.


METHODS
NSCs derived from the brain of E14.5d SD rat were cultured in vitro. Single cell suspensions were grafted into the striatum of the rat model of Parkinson's disease. Surviving animals were sacrificed at 10, 21, 35 and 60 d after transplantation, and the brain tissue was stained with HE and immunocytochemically to detect the expression of tyrosine hydroxylase (TH), CD4, CD8 and major histocompatibility complex class II antigens (MHC-II).


RESULTS
The expression of elicited CD4, CD8 and MHC-II was detected within and around the allografts in the graft groups at 10 and 21 d after grafting and was subsiding at 35 d. At 60 d only very occasional immunopositive cells were present. The number of TH positive neurons was low at 10, 21 d, but increased at 35 d and 60 d. There was no significant difference between negative and positive control groups at different time points. The rotation behavior of PD was decreased 35 d after transplantation.


CONCLUSIONS
Intracerebral transplantation of NSCs did not cause remarkable immunological rejection.
Zeng
Shui-lin
SL
Neurobiology Institute, Southeast University, Nanjing 210009, China. ZSL@seu.edu.cn
Han
Yang
Y
Wang
Lei
L
Lei
Zhi-nian
ZN
Zhu
Jian-bao
JB
Li
Tao
T
D000818:Animals; D001522:Behavior, Animal; D016380:Brain Tissue Transplantation / Q000276:immunology*; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000276:immunology* / Q000637:transplantation*; D010300:Parkinson Disease / Q000473:pathology / Q000628:therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D014184:Transplantation, Homologous / Q000276:immunology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D000949:Histocompatibility Antigens Class II