title: LOCATE: a mouse protein subcellular localization database.
abstract: We present here LOCATE, a curated, web-accessible database that houses data describing the membrane organization and subcellular localization of proteins from the FANTOM3 Isoform Protein Sequence set. Membrane organization is predicted by the high-throughput, computational pipeline MemO. The subcellular locations of selected proteins from this set were determined by a high-throughput, immunofluorescence-based assay and by manually reviewing >1700 peer-reviewed publications. LOCATE represents the first effort to catalogue the experimentally verified subcellular location and membrane organization of mammalian proteins using a high-throughput approach and provides localization data for approximately 40% of the mouse proteome. It is available at http://locate.imb.uq.edu.au.
authors.affiliation: ARC Centre in Bioinformatics, University of Queensland, St Lucia, Queensland 4072, Australia. l.fink@imb.uq.edu.au
authors.forename: J Lynn
authors.initials: JL
authors.lastname: Fink
authors.forename: Rajith N
authors.initials: RN
authors.lastname: Aturaliya
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Davis
authors.forename: Fasheng
authors.initials: F
authors.lastname: Zhang
authors.forename: Kelly
authors.initials: K
authors.lastname: Hanson
authors.forename: Melvena S
authors.initials: MS
authors.lastname: Teasdale
authors.forename: Chikatoshi
authors.initials: C
authors.lastname: Kai
authors.forename: Jun
authors.initials: J
authors.lastname: Kawai
authors.forename: Piero
authors.initials: P
authors.lastname: Carninci
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Rohan D
authors.initials: RD
authors.lastname: Teasdale
mesh_terms: D000818:Animals; D030562:Databases, Protein; D020407:Internet; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D014584:User-Computer Interface
chemical_list: D008565:Membrane Proteins; D020033:Protein Isoforms; D020543:Proteome