A differentially methylated region of the DAZ1 gene in spermatic and somatic cells.
OBJECTIVE
To investigate the methylation status of the deleted in azoospermia 1 (DAZ1) gene promoter region in different cell types.


METHODS
Using CpG island Searcher software, a CpG island was found in the promoter region of the DAZ1 gene. The methylation status of this region was analyzed in sperm and leukocytes by bisulfited sequencing.


RESULTS
The methylation status of the CpG island in the DAZ1 gene promoter region differed in leukocytes and sperm: it was methylated in leukocytes, but unmethylated in sperm.


CONCLUSIONS
A differentially methylated region of the DAZ1 gene exists in spermatic and somatic cells, suggesting that methylation of this region may regulate DAZ1 gene expression in different tissues.
Li
Zuo-Xiang
ZX
Department of Genetics, Peking Union Medical College, Beijing 100730, China.
Ma
Xu
X
Wang
Zhao-Hui
ZH
D001483:Base Sequence; D018899:CpG Islands; D004247:DNA / Q000737:chemistry; D019175:DNA Methylation; D000075364:Deleted in Azoospermia 1 Protein; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins / Q000235:genetics*; D013091:Spermatogenesis / Q000235:genetics; D013094:Spermatozoa / Q000737:chemistry / Q000378:metabolism*
C095668:DAZ1 protein, human; D000075364:Deleted in Azoospermia 1 Protein; D016601:RNA-Binding Proteins; D004247:DNA