Solubilization and quantification of lycopene in aqueous media in the form of cyclodextrin binary systems.
An optimized kneading method for the preparation of lycopene-cyclodextrin binary systems was developed leading to solubilization of lycopene in water and 5% (w/v) dextrose solution. Lycopene quantification in the prepared binary systems was performed by a developed spectrometric method that followed a successful single-step extraction with dichloromethane. Storage stability characteristics of the binary systems were studied at 4 degrees C in solution and at -20 degrees C in the lyophilized products. Lycopene content was monitored at lambda(max)=482 nm, the limit of detection was 0.41 microg/ml and relative standard deviation was less than 3.1%. The results obtained with the spectrometric method were confirmed by a HPLC method. In the presence of cyclodextrins, lycopene concentration in water was 8.0+/-1.0, 27.1+/-3.2 and 16.0+/-2.2 microg/ml for beta-CD, HP-beta-CD and Me-beta-CD, respectively. In 5% (w/v) aqueous dextrose solutions the corresponding values were 16.0+/-1.8, 48.0+/-5.1 and 4.0+/-0.5 microg/ml, respectively. At 4 degrees C, storage stability of lycopene-cyclodextrin binary systems in water or 5% (w/v) aqueous dextrose solutions, was limited (t(1/2)=1-4 days). Addition of the antioxidant sodium metabisulfite increased the stability of lycopene-HP-beta-CD binary system in water. At -20 degrees C, the lyophilized lycopene-cyclodextrin binary systems were stable for at least 2 weeks.
Vertzoni
Maria
M
Laboratory of Biopharmaceutics and Pharmacokinetics, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis, 15771 Athens, Greece.
Kartezini
Theodora
T
Reppas
Christos
C
Archontaki
Helen
H
Valsami
Georgia
G
D000975:Antioxidants / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D002338:Carotenoids / Q000032:analysis* / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D005612:Freeze Drying; D005947:Glucose / Q000737:chemistry; D000077276:Lycopene; D012995:Solubility; D013053:Spectrophotometry; D013447:Sulfites / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D013997:Time Factors; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000975:Antioxidants; D013447:Sulfites; D047392:beta-Cyclodextrins; D014867:Water; D002338:Carotenoids; C005200:sodium metabisulfite; D005947:Glucose; D000077276:Lycopene