Structure and mobility of lipid membranes on a thermoplastic substrate.
Supported lipid membranes constitute one of the most important model systems for cell membranes. The properties of lipid membranes supported by the hydrophobic solid polymer cyclic olefin copolymer (COC) were investigated. Lipid layers consisting of varying amounts of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP, cationic) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, neutral) prepared by vesicle fusion and solvent exchange were compared. All lipid mixtures coated the COC surface homogeneously forming a fluid membrane as verified by fluorescence microscopy and fluorescence recovery after photobleaching (FRAP). The exact structure of the supported membranes was determined by synchrotron reflectivity experiments using a microfluidic chamber. The X-ray data are in agreement with a compressed (head-to-head distance = 29 angstroms) and less densely packed bilayer.
Hochrein
Marion B
MB
Department für Physik, Ludwig-Maximilians-Universität München, Geschwister-Scholl-Platz 1, 80539 München, Germany. marion.hochrein@physik.uni-muenchen.de
Reich
Christian
C
Krause
Bärbel
B
Rädler
Joachim O
JO
Nickel
Bert
B
D002412:Cations / Q000737:chemistry; D003516:Cycloparaffins / Q000737:chemistry*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008561:Membrane Fusion; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques; D008856:Microscopy, Fluorescence; D044367:Phase Transition; D010713:Phosphatidylcholines / Q000737:chemistry; D038761:Photobleaching; D011108:Polymers / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties; D014961:X-Ray Diffraction
D002412:Cations; D003516:Cycloparaffins; D005229:Fatty Acids, Monounsaturated; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D012997:Solvents; C017251:1,2-oleoylphosphatidylcholine; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane