title: Expressed proteome analysis of human hepatocellular carcinoma in nude mice (LCI-D20) with high metastasis potential.
abstract: We report for the first time an expressed proteome for human hepatocellular carcinoma (HCC) in nude mice model. Most cases of human liver cancer are HCC with highly metastatic ability. Therefore, the early prediction or diagnosis and effective treatment are the key points of research. We have previously successfully established a human HCC nude mice model (LCI-D20) with high metastasis potential. To understand better the tumor biology of HCC it is worth to explore the relativity of all expressed protein profiles in the LCI-D20 HCC nude mice model. With advanced proteomics technologies, we have carried out a proteomic analysis with following stages: protein sample preparation of cancer tissue, including total cellular extraction and sequential fractionation, 2-DE and 2-D LC separation, ESI/MALDI-MS/MS identification, as well as data-dependent bioinformatics. The identified proteins were classified bioinformatically respective to their function, biological process and intracellular localization. Some important proteins found in HCC, e.g. metabolism enzymes, proteins regulating cell motility, signaling proteins, and heat shock proteins, are discussed in terms of their metastasis.
authors.affiliation: Institutes for Biomedical Sciences, Fudan University, Shanghai, P R China.
authors.forename: Huali
authors.initials: H
authors.lastname: Shen
authors.forename: Gang
authors.initials: G
authors.lastname: Cheng
authors.forename: Huizhi
authors.initials: H
authors.lastname: Fan
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
authors.forename: Haojie
authors.initials: H
authors.lastname: Lu
authors.forename: Chunli
authors.initials: C
authors.lastname: Liu
authors.forename: Fengxia
authors.initials: F
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Xu
authors.forename: Guobin
authors.initials: G
authors.lastname: Xu
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.forename: Caiyun
authors.initials: C
authors.lastname: Fang
authors.forename: Huimin
authors.initials: H
authors.lastname: Bao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Zhong
authors.forename: Ziniu
authors.initials: Z
authors.lastname: Yu
authors.forename: Yinkun
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhaoyou
authors.initials: Z
authors.lastname: Tang
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000556:secondary; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000378:metabolism*; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured
chemical_list: D009363:Neoplasm Proteins; D020543:Proteome