[Study on Haemophilus influenzae type b: data from autopsy of community acquired pneumonia among children].
OBJECTIVE
To evaluate the status of Haemophilus influenza type b(Hib) on death cases of children from community-acquired pneumonia (CAP) and to estimate the value of direct in-situ polymerase chain reaction (ISPCR) on diagnosis of children CAP, pathogenically.


METHODS
Ordinary PCR, Southern blot and direct ISPCR were applied and compared in detecting Hib in 100 paraffin-embedded lung tissues of autopsy children died of CAP.


RESULTS
No major difference on the detection rate of Hib between 50-60s and 80s-2002 was found. The detection rate of Hib by direct ISPCR was higher than the other two methods. By Southern blot, Hib was identified from 8 out of 100 samples (8%), including 4 out of 56 in 1950-60s (7.1%) and 4 out of 44 (9.1%) (chi2 = 0.084, P>0.05) in 1980s-2002. By ISPCR, Hib was identified from 17 out of 100 samples (17%), including 8 out of 56 in 1950-60s (14.3%) and 9 out of 44 (20.5%) with chi2 = 0.665, P > 0.05, in 1980s-2002. Positive cases diagnosed by both Southern blot and ISPCR were 7%.


CONCLUSIONS
Hib was one of the main bacterial pathogens causing CAP and deaths among children. Direct ISPCR was prefertable to be used in pathogenic diagnosis on children pneumonia, in terms of its sensitivity, specificity and localization.
Hu
Hui-li
HL
Laboratory of Microbiology and Immunology, Beijing Children's Hospital Affiliated to Capital University of Medical Science, Beijing 100045, China.
Hu
Yi-yun
YY
He
Le-jian
LJ
Yu
Sang-jie
SJ
Gao
Wei
W
Yang
Yong-hong
YH
D000367:Age Factors; D001344:Autopsy; D015139:Blotting, Southern; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000382:microbiology / Q000473:pathology; D005260:Female; D019741:Haemophilus influenzae type b / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007223:Infant; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008297:Male; D011014:Pneumonia / Q000382:microbiology* / Q000473:pathology*; D016133:Polymerase Chain Reaction