[The dynamic study between EBV-DNA with nasopharyngeal carcinoma].
OBJECTIVE
To study the varies of blood plasma Epstein-Barr virus (EBV) DNA before and after radiotherapy in nasopharyngeal carcinoma and the relationship between it with the tumor growth and decline, residual and relapse as well as metastasis.


METHODS
Polymerase chain reaction (PCR) with restriction enzyme technique was used to detect EBV-DNA.


RESULTS
Seventy-one out of 74 specimens were EBV-DNA positive, the positive rate was 95.9% (71/74). In the time of radiation dose 50 Gy/5 week, the primary nasopharynx tumors and lymph node of neck in 23 cases disappeared, the positive rate was 13.0% (3/23). In the group of tumors remained, the positive rate was 62.7% (32/51). In the time of radiation dose 70 Gy/7 week, 7 out of 74 existed residual tumor after radiotherapy, 5 out of 7 cases were positive, the positive rate was 71.4% (5/7). In the cases the tumors disappeared, EBV-DNA was negative. Eleven out of 12 cases with relapse after radiotherapy EBV-DNA fragments detected were positive. All of 8 patients with metastasis after radiotherapy, their EBV-DNA fragments detected were positive.


CONCLUSIONS
The detection of blood plasma EBV-DNA can reflect the tumors growth and decline well. It is an important and sensitive index in diagnosing the residual and relapse as well as the metastasis of nasopharyngeal carcinoma.
Jiang
Wuzhong
W
Department of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, China. jwz903@sohu.com
Liao
Yuping
Y
D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000000981:diagnostic imaging / Q000821:virology*; D011859:Radiography
D004279:DNA, Viral