title: Analysis of genetic polymorphism of deoxyribonuclease I in Ovambo and Turk populations using a genotyping method.
abstract: Deoxyribonuclease I (DNase I) polymorphism has been used as a valuable marker in genetic and clinical investigations. Six codominant alleles are known for DNase I, DNASE1*1, *2, *3, *4, and the recently discovered alleles *5 and *6. To detect these two new alleles, we added a new DNase I genotyping method based on both an allele-specific amplification and mismatched polymerase chain reaction (PCR). These methods were used to examine the distribution of DNase I genotypes in unrelated individuals from bloodstains of Ovambo and Turkish populations. The DNASE1*1 allele was found to be most dominant in the Ovambos. In contrast, Turks showed the highest allele frequency for DNASE1*2. This study is the first to demonstrate that there is a certain genetic heterogeneity in the worldwide distribution of DNase I polymorphism using the genotyping method of human DNase I polymorphism with PCR.
authors.affiliation: Department of Legal Medicine, Shimane University School of Medicine, Izumo, Shimane 693-8501, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Fujihara
authors.forename: Yoko
authors.initials: Y
authors.lastname: Hieda
authors.forename: Koji
authors.initials: K
authors.lastname: Takayama
authors.forename: Yuying
authors.initials: Y
authors.lastname: Xue
authors.forename: Norihito
authors.initials: N
authors.lastname: Nakagami
authors.forename: Shinji
authors.initials: S
authors.lastname: Imamura
authors.forename: Kaori
authors.initials: K
authors.lastname: Kataoka
authors.forename: Haruo
authors.initials: H
authors.lastname: Takeshita
mesh_terms: D044466:Asian People / Q000235:genetics*; D044383:Black People / Q000235:genetics*; D017931:DNA Primers; D003850:Deoxyribonuclease I / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D009276:Namibia; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D014421:Turkey
chemical_list: D017931:DNA Primers; D003850:Deoxyribonuclease I