Estimation of p-coumaric acid as metabolite of E-6-O-p-coumaroyl scandoside methyl ester in rat plasma by HPLC and its application to a pharmacokinetic study.
A rapid and simple high-performance liquid chromatographic (HPLC) method has been developed for the determination of p-coumaric acid in rat plasma and applied to a pharmacokinetic study in rats after administration of a prodrug, E-6-O-p-coumaroyl scandoside methyl ester, isolated from Hedyotis diffusa (Willd.). Sample preparation involved protein precipitation with acetonitrile. The supernatant was then injected onto a Diamonsil C(18) column (250 mm x 4.6mm i.d., 5 microm). The mobile phase consisted of acetonitrile-water (21:79, v/v) with 1% glacial acetic acid. The UV detector was set at 310 nm. The lower limit of quantification of p-coumaric acid in rat plasma was 0.02 microg/mL. The calibration curves were linear over the concentration range 0.02-5 microg/mL with correlations greater than 0.999. The assay procedure was applied to the study of the metabolite pharmacokinetics of E-6-O-p-coumaroyl scandoside methyl ester in rat.
Liu
Ke
K
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province 110016, PR China.
Yan
Linqi
L
Yao
Guocan
G
Guo
Xinjie
X
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000097:blood* / Q000493:pharmacokinetics*; D004952:Esters / Q000493:pharmacokinetics*; D011355:Prodrugs / Q000493:pharmacokinetics; D011422:Propionates; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
D003373:Coumaric Acids; C509054:E-6-O-p-coumaroyl scandoside methyl ester; D004952:Esters; D011355:Prodrugs; D011422:Propionates; C495469:p-coumaric acid