title: Cloning, characterization and expression of a Lateral suppressor-like gene from chrysanthemum (Dendranthema grandiflorum Kitamura).
abstract: Conventionally, the lateral shoots of chrysanthemum are removed manually, which is time consuming and uneconomical. The development of branchless chrysanthemum will economize its commercial cultivation. To investigate the regulatory mechanism of branchlessness, we undertook cloning of Dendranthema grandiflorum Kitamura Lateral suppressor-like (DgLsL) gene for development of lateral shoot in chrysanthemum. A full-length cDNA of DgLsL gene was isolated by screening cDNA library and performing Rapid Amplification of cDNA Ends (RACE) PCR. Phylogenetic analysis showed that the DgLsL gene is closely related to Lateral suppressor that encodes transcriptional regulator proteins belonging to GRAS family of known transcription factor. Southern blot analysis revealed that DgLsL gene in chrysanthemum genome has one copy. DgLsL expression was apparently up-regulated by ethephon treatment. The expression patterns revealed that DgLsL transcripts were detected in all organs, but showed their highest level in stems.
authors.affiliation: Department of Horticulture, College of Agriculture and Life Science, Chungnam National University, 220 Kung-Dong, Yusong-Gu, Daejeon 305-764, Korea.
authors.forename: Dae Hwa
authors.initials: DH
authors.lastname: Yang
authors.forename: Pil-Yong
authors.initials: PY
authors.lastname: Yun
authors.forename: So Young
authors.initials: SY
authors.lastname: Park
authors.forename: Prikshit
authors.initials: P
authors.lastname: Plaha
authors.forename: Dong Sook
authors.initials: DS
authors.lastname: Lee
authors.forename: In Sok
authors.initials: IS
authors.lastname: Lee
authors.forename: Yong Su
authors.initials: YS
authors.lastname: Hwang
authors.forename: Young A
authors.initials: YA
authors.lastname: Kim
authors.forename: Jong Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Bong Hee
authors.initials: BH
authors.lastname: Han
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Suh
authors.forename: Yong Pyo
authors.initials: YP
authors.lastname: Lim
mesh_terms: D000595:Amino Acid Sequence; D015139:Blotting, Southern; D031202:Chrysanthemum / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D015723:Gene Library; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
chemical_list: D009943:Organophosphorus Compounds; D010937:Plant Growth Regulators; C005073:ethephon