Isolation of poly-3-hydroxybutyrate metabolism genes from complex microbial communities by phenotypic complementation of bacterial mutants.
The goal of this study was to initiate investigation of the genetics of bacterial poly-3-hydroxybutyrate (PHB) metabolism at the community level. We constructed metagenome libraries from activated sludge and soil microbial communities in the broad-host-range IncP cosmid pRK7813. Several unique clones were isolated from these libraries by functional heterologous complementation of a Sinorhizobium meliloti bdhA mutant, which is unable to grow on the PHB cycle intermediate D-3-hydroxybutyrate due to absence of the enzyme D-3-hydroxybutyrate dehydrogenase activity. Clones that conferred D-3-hydroxybutyrate utilization on Escherichia coli were also isolated. Although many of the S. meliloti bdhA mutant complementing clones restored D-3-hydroxybutyrate dehydrogenase activity to the mutant host, for some of the clones this activity was not detectable. This was also the case for almost all of the clones isolated in the E. coli selection. Further analysis was carried out on clones isolated in the S. meliloti complementation. Transposon mutagenesis to locate the complementing genes, followed by DNA sequence analysis of three of the genes, revealed coding sequences that were broadly divergent but lay within the diversity of known short-chain dehydrogenase/reductase encoding genes. In some cases, the amino acid sequence identity between pairs of deduced BdhA proteins was <35%, a level at which detection by nucleic acid hybridization based methods would probably not be successful.
Wang
Chunxia
C
Department of Biology, University of Waterloo, 200 University Ave. West, Waterloo, ON N2L 3G1, Canada.
Meek
David J
DJ
Panchal
Priya
P
Boruvka
Natalie
N
Archibald
Frederick S
FS
Driscoll
Brian T
BT
Charles
Trevor C
TC
D003001:Cloning, Molecular; D003589:Cytophaga / Q000235:genetics; D015723:Gene Library; D005816:Genetic Complementation Test; D006090:Gram-Negative Bacteria / Q000235:genetics; D006884:Hydroxybutyrate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D011091:Polyesters / Q000378:metabolism*; D020560:Proteobacteria / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D016962:Sinorhizobium meliloti / Q000201:enzymology / Q000235:genetics; D012988:Soil Microbiology; D000094122:Polyhydroxybutyrates
D006884:Hydroxybutyrate Dehydrogenase; D006885:Hydroxybutyrates; D011091:Polyesters; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates