title: Genetic diversity in Hemileia vastatrix based on RAPD markers.
abstract: Random amplified polymorphic DNA (RAPD) was used to assess the genetic structure of Hemileia vastatrix populations. Forty-five rust isolates with different virulence spectra and from different hosts and geographical regions were analyzed. Out of 45 bands, generated with three RAPD primers, 35 (78%) were polymorphic and scored as molecular markers. Cluster analysis exhibits unstructured variability of this pathogen with regard to physiological race, geographical origin or host. The genotypic diversity (H') inferred from Shannon's index was higher than gene diversity (Ht), suggesting that diversity is distributed among clonal lineages. Estimates of gene diversity in Africa and Asia populations were higher in total (Ht) as compared to within population diversity (Hs). Genetic differentiation was considerable among coffee rust isolates from Africa (Gst = 0.865) and Asia (Gst = 0.768) but not among isolates from South America (Gst = 0.266). We concluded that genetic diversity in H. vastatrix was moderately low and that the genetic differentiation among populations shows that asexual reproduction is likely to play an important role in the population biology of this fungus. This should be taken into account for the development of breeding programs.
authors.affiliation: Centro de Investigação das Ferrugens do Cafeeiro, Instituto de Investigação Científica Tropical, Quinta do Marqués, 2784-505 Oeiras, Portugal. mgouveia@uma.pt
authors.forename: M Manuela C
authors.initials: MM
authors.lastname: Gouveia
authors.forename: Ana
authors.initials: A
authors.lastname: Ribeiro
authors.forename: Vítor M P
authors.initials: VM
authors.lastname: Várzea
authors.forename: Carlos J
authors.initials: CJ
authors.lastname: Rodrigues
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D040503:Coffea / Q000382:microbiology; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis / Q000378:metabolism; D014644:Genetic Variation; D005843:Geography; D010935:Plant Diseases / Q000382:microbiology; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004271:DNA, Fungal