title: N-acetyltransferase 2 polymorphism in patients with diabetes mellitus.
abstract: The arylamine N-acetyltransferases (NATs) are a unique family of enzymes that catalyse the transfer of an acetyl group from acetyl-CoA to the terminal nitrogen of hydrazine and arylamine drugs and carcinogens. Human arylamine NATs are known to exist as two isoenzymes, NAT1 and NAT2. The objective of this study was to identify whether the genetic polymorphism of NAT2 plays a role in susceptibility to Diabetes Mellitus (DM). Ninety-seven patients with DM and 104 healthy controls were enrolled in the study. NAT2*5A, NAT2*6A, NAT2*7A/B and NAT2*14A polymorphisms were detected by using real time PCR with LightCycler (Roche Diagnostics GmbH, Mannheim, Germany). According to our data, the NAT2*5A and NAT2*6A mutant genotypes and NAT2*14A heterozygous genotype were associated with an increased risk of development of DM (OR = 47.06; 95%CI: 10.55-209.77 for NAT 2*5A, OR = 18.48; 95%CI: 3.83-89.11 for NAT2*6A and OR = 18.22; 95%CI: 6.29-52.76 for NAT2*14A). However, the NAT2*7A/B gene polymorphism carried no increased risk for developing DM disease. After grouping according to phenotypes as either slow or fast acetylators, NAT2*6A slow acetylator was found to be a significant risk factor for DM (OR = 6.09; 95%CI: 1.99-18.6, p = 0.02). The results indicate that NAT2 slow acetylator genotypes may be an important genetic determinant for DM in the Turkish population.
authors.affiliation: Mersin University Pharmacy School, Department of Biochemistry, Mersin, Turkey. syalin01@hotmail.com
authors.forename: Serap
authors.initials: S
authors.lastname: Yalin
authors.forename: Rezan
authors.initials: R
authors.lastname: Hatungil
authors.forename: Lulufer
authors.initials: L
authors.lastname: Tamer
authors.forename: Nurcan Aras
authors.initials: NA
authors.lastname: Ates
authors.forename: Nil
authors.initials: N
authors.lastname: Dogruer
authors.forename: Hatice
authors.initials: H
authors.lastname: Yildirim
authors.forename: Sevim
authors.initials: S
authors.lastname: Karakas
authors.forename: Ugur
authors.initials: U
authors.lastname: Atik
mesh_terms: D000328:Adult; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D003920:Diabetes Mellitus / Q000201:enzymology* / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011110:Polymorphism, Genetic
chemical_list: D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human