title: Aged garlic extract induces proliferation and ameliorates gentamicin-induced toxicity in LLC-PK1 cells.
abstract: Gentamicin (GM)-induced nephrotoxicity limits the use of this antibiotic. It has been shown that aged garlic extract (AGE) and S-allylcysteine (SAC), the most abundant organosulfur compound in AGE, ameliorate GM-induced nephrotoxicity in rats. The present communication evaluated the effect of AGE and SAC on proliferation and on GM-induced toxicity and genotoxicity of porcine kidney epithelial cell line (LLC-PK1 cells). The cells were preincubated with different concentrations of AGE or SAC for 12 h before incubation with 8 mm GM for an additional 72 h. At the end of this time, cell viability, genotoxicity and proliferation were evaluated. AGE stimulated cell proliferation and protected LLC-PK1 cells from GM-mediated toxicity and genotoxicity. SAC partially prevented only GM-induced genotoxicity. These results suggest that the stimulation of cell proliferation could possibly be one of the mechanisms involved in the in vitro protective effect of AGE in GM-induced toxicity of LLC-PK1 cells.
authors.affiliation: Departamento de Farmacología, Facultad de Medicina, UNAM, Mexico City, Mexico.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Velasco-Velázquez
authors.forename: Perla D
authors.initials: PD
authors.lastname: Maldonado
authors.forename: Diana
authors.initials: D
authors.lastname: Barrera
authors.forename: Víctor
authors.initials: V
authors.lastname: Torres
authors.forename: Alejandro
authors.initials: A
authors.lastname: Zentella-Dehesa
authors.forename: José
authors.initials: J
authors.lastname: Pedraza-Chaverrí
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004797:Enzyme-Linked Immunosorbent Assay; D005737:Garlic / Q000737:chemistry; D005839:Gentamicins / Q000633:toxicity*; D018374:LLC-PK1 Cells; D009152:Mutagenicity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D018675:Toxicity Tests
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D010936:Plant Extracts; D020011:Protective Agents; C065299:S-allylcysteine; D001973:Bromodeoxyuridine; D003545:Cysteine