title: Noninvasive measurement of ablation crater size and thermal injury after CO2 laser in the vocal cord with optical coherence tomography.
abstract: OBJECTIVE
  To characterize tissue destruction after CO(2) laser-ablation of the vocal cords with the use of optical coherence tomography (OCT).
  
  
  METHODS
  OCT was used to image fresh porcine vocal cords after laser ablation. OCT and histology estimates of the ablation crater dimensions and the depth of thermal injury were obtained.
  
  
  RESULTS
  The vocal cord substructures up to 2.29 mm in depth at 10 microm resolution, and the thermal disruption after laser ablation were identified by OCT. OCT and histology estimates of the lesion dimensions showed no significant differences. Crater depth is directly proportional to laser power, whereas crater width and the zone of thermal injury appear to be unrelated to laser power.
  
  
  CONCLUSIONS
  OCT may be used to accurately characterize the native states and the laser-induced thermal injury of laryngeal mucosa, within the inherent limitation in its depth of penetration. OCT may be a useful diagnostic and monitoring tool in an otolaryngology practice.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Beckman Laser Institute, University of California-Irvine, 1002 Health Sciences Road, Irvine, CA 92612, USA.
authors.forename: Behrooz A
authors.initials: BA
authors.lastname: Torkian
authors.forename: Shuguang
authors.initials: S
authors.lastname: Guo
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Jahng
authors.forename: Lih-Huei L
authors.initials: LH
authors.lastname: Liaw
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Chen
authors.forename: Brian J F
authors.initials: BJ
authors.lastname: Wong
mesh_terms: D000818:Animals; D002056:Burns / Q000000981:diagnostic imaging* / Q000209:etiology / Q000473:pathology; D007820:Laryngeal Mucosa / Q000000981:diagnostic imaging / Q000293:injuries / Q000473:pathology; D007834:Lasers; D011859:Radiography; D015203:Reproducibility of Results; D013552:Swine; D046509:Tissue Culture Techniques; D041623:Tomography, Optical Coherence; D014827:Vocal Cords / Q000000981:diagnostic imaging* / Q000293:injuries* / Q000473:pathology