[Determination of fleroxacin by photochemical fluorescence of zinc-fleroxacin complex].
Based on the complex formed by Zn2+ which can strengthen the relative fluorescence intensity of fleroxacin evidently, a novel photochemical fluorescence method was developed. The effects of the acidity, the concentration ratio of Zn2+ to fleroxacin, and the time for illumination were studied. Under the optimum experiment conditions, the linear range of the determination was 5.00 x 10(-8) - 5.00 x 10(-6) mol x L(-1). The detection limit was 4.2 x 10(-8) mol x L(-1). The relative standard deviation of the determination of fleroxacin (5.0 x 10(-7) mol x L(-1)) was 1.7% (n = 20). The method was successfully applied to the determination of fleroxacin in specimens, and the recoveries were in the range of 95.0%-105%. The mechanism of this system is proposed.
Feng
Rui-qin
RQ
Department of Chemistry, Beijing Normal University, Beijing 100875, China.
Ding
Fen
F
Liu
Yu
Y
D016576:Fleroxacin / Q000032:analysis* / Q000737:chemistry; D005453:Fluorescence; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry; D055668:Photochemical Processes; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000737:chemistry*
D009942:Organometallic Compounds; D015032:Zinc; D016576:Fleroxacin