title: Gene expression profiling identifies potential relevant genes in alveolar rhabdomyosarcoma pathogenesis and discriminates PAX3-FKHR positive and negative tumors.
abstract: We analyzed the expression signatures of 14 tumor biopsies from children affected by alveolar rhabdomyosarcoma (ARMS) to identify genes correlating to biological features of this tumor. Seven of these patients were positive for the PAX3-FKHR fusion gene and 7 were negative. We used a cDNA platform containing a large majority of probes derived from muscle tissues. The comparison of transcription profiles of tumor samples with fetal skeletal muscle identified 171 differentially expressed genes common to all ARMS patients. The functional classification analysis of altered genes led to the identification of a group of transcripts (LGALS1, BIN1) that may be relevant for the tumorigenic processes. The muscle-specific microarray platform was able to distinguish PAX3-FKHR positive and negative ARMS through the expression pattern of a limited number of genes (RAC1, CFL1, CCND1, IGFBP2) that might be biologically relevant for the different clinical behavior and aggressiveness of the 2 ARMS subtypes. Expression levels for selected candidate genes were validated by quantitative real-time reverse-transcription PCR.
authors.affiliation: CRIBI Biotechnology Centre and Dipartimento di Biologia, Università degli Studi di Padova, Padova, Italy.
authors.forename: Cristiano
authors.initials: C
authors.lastname: De Pittà
authors.forename: Lucia
authors.initials: L
authors.lastname: Tombolan
authors.forename: Giada
authors.initials: G
authors.lastname: Albiero
authors.forename: Francesca
authors.initials: F
authors.lastname: Sartori
authors.forename: Chiara
authors.initials: C
authors.lastname: Romualdi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Jurman
authors.forename: Modesto
authors.initials: M
authors.lastname: Carli
authors.forename: Cesare
authors.initials: C
authors.lastname: Furlanello
authors.forename: Gerolamo
authors.initials: G
authors.lastname: Lanfranchi
authors.forename: Angelo
authors.initials: A
authors.lastname: Rosolen
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000032:analysis / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D007223:Infant; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors / Q000032:analysis / Q000235:genetics*; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018232:Rhabdomyosarcoma, Alveolar / Q000235:genetics* / Q000503:physiopathology*
chemical_list: C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D000072041:PAX3 Transcription Factor; C489409:PAX3 protein, human; D051761:Paired Box Transcription Factors