title: Measurement of monomolecular binding constants of neutral phenols into the beta-cyclodextrin by continuous frontal analysis in capillary and microchip electrophoresis via a competitive assay.
abstract: Measurement of binding constant by chip electrophoresis is a very promising technique for the high throughput screening of non-covalent interactions. Among the different electrophoretic methods available that yield the binding parameters, continuous frontal analysis is the most appropriate for a transposition from capillary electrophoresis (CE) to microchip electrophoresis. Implementation of this methodology in microchip was exemplified by the measurement of inclusion constants of 2-naphtalenesulfonate and neutral phenols (phenol, 4-chlorophenol and 4-nitrophenol) into beta-cyclodextrin by competitive assays. The issue of competitor choice is discussed in relation to its appropriateness for proper monitoring of the interaction.
authors.affiliation: Graduate School of Material Science, University of Hyogo, Kamigori, Hyogo 697-1297 Japan. Thomas.LeSaux@ens.fr
authors.forename: Thomas
authors.initials: T
authors.lastname: Le Saux
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hisamoto
authors.forename: Shigeru
authors.initials: S
authors.lastname: Terabe
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D010636:Phenols / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D010636:Phenols; D047392:beta-Cyclodextrins; C031215:betadex