Relationship between SUMO-1 modification of caspase-7 and its nuclear localization in human neuronal cells.
The aim of this study was to elucidate the role of SUMO-1 modification in caspase-7 in neuronal cells. We have previously demonstrated that procaspase-2 could be a possible target for SUMO-1 modification. In the present study, we attempted to investigate whether other caspases also interact with Ubc9/SUMO-1. The specific binding of SUMO-1 with caspase-7 was observed in mammalian cells. Deletion mutant analysis revealed that a SUMO-1 modification site may be located in at least N-terminal p20 subunit of caspase-7. Furthermore, SUMO-1-modified caspase-7 appeared as a dot-like structure in nuclear localization. These findings suggest that SUMO-1 modification in caspase-7 may be linked to specific its localization in the nucleus and may therefore contribute to the cleavage of nuclear substrates during neuronal apoptosis.
Hayashi
Naoko
N
Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
Shirakura
Hiromi
H
Uehara
Takashi
T
Nomura
Yasuyuki
Y
D015153:Blotting, Western / Q000379:methods; D053179:Caspase 7; D020169:Caspases / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005455:Fluorescent Antibody Technique / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000502:physiology; D025842:SUMO-1 Protein / Q000378:metabolism*; D019311:Staurosporine / Q000494:pharmacology; D014162:Transfection / Q000379:methods
D004791:Enzyme Inhibitors; D025842:SUMO-1 Protein; D049452:Green Fluorescent Proteins; C505520:CASP7 protein, human; D053179:Caspase 7; D020169:Caspases; D019311:Staurosporine