title: Non-glucan attached proteins of Candida albicans biofilm formed on various surfaces.
abstract: Non-glucan attached proteins of the cell surface and extracellular matrix of Candida albicans biofilms formed on two catheter surfaces and denture acrylic were examined. The SDS-PAGE protein profiles of these proteins compared with that obtained from planktonic yeast cells and germ tubes were generally similar. This observation suggested that this class of biofilm surface proteins is not composed of a unique set of extracellular proteins or that one or a few proteins dominate the non-glucan attached proteins of biofilm. However, differences were observed in the proteins obtained from biofilm formed on one catheter surface and two proteins, Grp2p and ORF19.822p, identified by mass spectrometry following two-dimensional separation. These proteins have previously been associated with drug resistance and their presence or abundance appeared to be influenced by the surface on which the biofilm was formed.
authors.affiliation: Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA.
authors.forename: Govindsamy
authors.initials: G
authors.lastname: Vediyappan
authors.forename: W Lajean
authors.initials: WL
authors.lastname: Chaffin
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D015153:Blotting, Western; D002176:Candida albicans / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D002404:Catheterization; D003778:Dentures; D004351:Drug Resistance; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000502:physiology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D016459:Prosthesis-Related Infections / Q000382:microbiology*
chemical_list: D005656:Fungal Proteins