title: [Study on the collecting method of constituents absorbed into the blood from anti-V capsule].
abstract: OBJECTIVE
  Aim To establish the condition and parameters for collecting the effective constituents of Anti-V capsule.
  
  
  METHODS
  Based on HPLC, using chlorogenic acid and baicalin which are the main constituents absorbed in the blood as the marker substance, the best technical process of macropore absorbed resin was evaluated by the quantity of two compounds in the collected part.
  
  
  RESULTS
  After the abstraction of the capsule, the collection method of NKA-2 macropore absorbed resin as sorbent and 5 bed volume 50% ethanol as mobile phase was definited as the best craftwork. In the collected part, the content of chlorogenic acid and baicalin was five times than in the capsule.
  
  
  CONCLUSIONS
  In guide of serum pharmacochemistry, macropore absorbed resin is the effective method to collect the constituents absorbed in blood of Anti-V capsule. At the same time, this method provides a way to research other Compound medicine.
authors.affiliation: Pharmacy Department, Heilongjiang University of Chinese Medicine, Harbin 150040, China. xijunw@sina.com
authors.forename: Xi-jun
authors.initials: XJ
authors.lastname: Wang
authors.forename: Ningi
authors.initials: N
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Hong-xin
authors.initials: HX
authors.lastname: Cao
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Sun
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification; D002214:Capsules; D002726:Chlorogenic Acid / Q000097:blood / Q000302:isolation & purification*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005260:Female; D005419:Flavonoids / Q000097:blood / Q000302:isolation & purification*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012117:Resins, Synthetic; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000998:Antiviral Agents; D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012117:Resins, Synthetic; D002726:Chlorogenic Acid; C038044:baicalin