Cloning and genomic characterization of sytdep, a new synaptotagmin XIV-related gene.
We have identified a new human gene coined sytdep (synaptotagmin XIV-derived protein) in human neutrophils. Sytdep encodes a 188-amino acid sequence with a 21.435kDa deduced molecular mass, showing 75% identity to human synaptotagmin (syt) XIV. Human neutrophils express sytdep, but not syt XIV. Sytdep was upregulated during HL-60 neutrophil differentiation. Sytdep gene is located in human chromosome 4 and contains a unique exon, whereas syt XIV gene, located in chromosome 1, comprises 10 exons with 9 introns. Mouse genome did not contain sytdep. The N-terminal region of sytdep shows no homology with any known protein and, unlike synaptotagmin XIV isoforms, sytdep shows a unique C-terminal C2B domain. Polyclonal antibodies against the C2B domain of syt XIV recognized sytdep as a 27-kDa protein in human neutrophils. Genomic analyses suggest that human sytdep could derive from a retrotranslocation of a syt XIV transcript into chromosome 4.
Herrero-Turrión
M Javier
MJ
Centro de Investigación del Cáncer, Instituto de Biología Molecular y Celular del Cáncer, Consejo Superior de Investigaciones Científicas (C.S.I.C.)-Universidad de Salamanca, Campus Miguel de Unamuno, E-37007 Salamanca, Spain.
Fukuda
Mitsunori
M
Mollinedo
Faustino
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular / Q000379:methods; D015894:Genome, Human; D018922:HL-60 Cells; D006801:Humans; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000737:chemistry / Q000378:metabolism; D017422:Sequence Analysis, DNA; D050857:Synaptotagmins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014178:Translocation, Genetic
D002135:Calcium-Binding Proteins; C508557:SYT14P1 protein, human; D050857:Synaptotagmins