title: pH triggered release of protective poly(ethylene glycol)-b-polycation copolymers from liposomes.
abstract: Triggered release of adsorbed polymers from liposomes enables protection against immune recognition during circulation and subsequent intracellular delivery of DNA. Polycationic blocks, poly[2-(dimethylamino) ethyl methacrylate] (DMAEMA) (0.8, 3.1, 4.9, or 9.8 kg/mol) or polylysine (K) (3 kg/mol), act as anchors for poly(ethylene glycol) (PEG) (2 or 5 kg/mol) protective blocks. In addition, a copolymer with 15 strictly alternating blocks of PEG (2 kg/mol) and cationic amine sites was evaluated as a protective coating. Incorporation of 1,2-dioleoyl-3-dimethylammonium-propane, a titratable lipid with a pKa of approximately 6.7, allows the liposome's net charge to increase as the pH shifts from 7.4 in the bloodstream to 5.5 in the endosome. The increased net liposome cationicity results in decreased cationic polymer adsorption. The EMPEG113-DMAEMA31 and EMPEG113-DMAEMA62 association constants decrease from 3.1 and 6.2 (mg/m(2))/(mg/ml) at pH 7.4 to 1.7 and 3.2 (mg/m(2))/(mg/ml) at pH 5.5, respectively. However, EMPEG45-DMAEMA5, EMPEG45-DMAEMA20, and EMPEG45-N-DP15 did not show a strong response to changes in pH. Cationic polymer adsorption exceeds calculated values for liposome neutralization, resulting in adsorption profiles in the brush regime.
authors.affiliation: Department of Chemical Engineering, Princeton University, Engineering Quadrangle, Princeton, NJ 08544, USA.
authors.forename: Debra T
authors.initials: DT
authors.lastname: Auguste
authors.forename: Steven P
authors.initials: SP
authors.lastname: Armes
authors.forename: Krystyna R
authors.initials: KR
authors.lastname: Brzezinska
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Deming
authors.forename: Joachim
authors.initials: J
authors.lastname: Kohn
authors.forename: Robert K
authors.initials: RK
authors.lastname: Prud'homme
mesh_terms: D000327:Adsorption; D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D018014:Gene Transfer Techniques; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D002412:Cations; D008081:Liposomes; D008689:Methacrylates; D011092:Polyethylene Glycols; D004247:DNA; C016439:ethyl methacrylate monomer