title: FR177391, a new anti-hyperlipidemic agent from Serratia. III. Microbial conversion of FR177391 and synthesis of FR177391 derivatives for its target protein screening by chemical genetic approaches.
abstract: FR177391 produced by Serratia liquefaciens No. 1821 enhances differentiation of mouse 3T3-L1 fibroblasts to adipocytes and reduces the circulating levels of triglyceride in C57BL/KsJ-db/bd mice, an obese non-insulin-dependent diabetes mellitus animal model, although its mechanism of actions remained to be unknown. Its active derivative, 20-hydroxy FR177391, and its inactive derivative, 3-hydroxy FR177391 were produced by microbial conversion of FR177391, and biotin-labeled FR177391 was synthesized from 20-hydroxy FR177391 as an active affinity ligand to identify target molecules of FR177391 by chemical genetic approaches.
authors.affiliation: Exploratory Research Laboratories, Fujisawa Pharmaceutical Co Ltd, Tsukuba, Ibaraki, Japan. motoo.kobayashi@jp.astellas.com
authors.forename: Motoo
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Sato
authors.forename: Seiji
authors.initials: S
authors.lastname: Yoshimura
authors.forename: Makiko
authors.initials: M
authors.lastname: Yamaoka
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Takase
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Ohkubo
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujii
authors.forename: Hidenori
authors.initials: H
authors.lastname: Nakajima
mesh_terms: D000960:Hypolipidemic Agents / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D012705:Serratia / Q000737:chemistry*
chemical_list: D000960:Hypolipidemic Agents