Regeneration of plants from alginate-encapsulated shoot tips of Withania somnifera (L.) Dunal, a medicinally important plant species.
A protocol was developed for plant regeneration from encapsulated shoot tips collected from in vitro proliferated shoots of Withania somnifera. The best gel composition was achieved using 3% sodium alginate and 75 mM CaCl2.2H2O. The maximum percentage response (87%) for conversion of encapsulated shoot tips into plantlets was achieved on MS medium supplemented with 0.5 mg/l IBA after 5 weeks of culture. The conversion of encapsulated shoot tips into plantlets also occurred when calcium alginate beads having entrapped propagules were directly sown in autoclaved soilrite moistened with 14-MS salts.
Singh
Ajay K
AK
National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi-110 012, India.
Varshney
Rachna
R
Sharma
Manu
M
Agarwal
Shyam S
SS
Bansal
Kailash C
KC
D000464:Alginates; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D018520:Plant Shoots / Q000502:physiology*; D010946:Plants, Medicinal; D012038:Regeneration / Q000502:physiology*; D014021:Tissue Preservation / Q000379:methods*; D032341:Withania / Q000254:growth & development*
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid