In vitro and in vivo anti-inflammatory activity of a seed preparation containing phenethylisothiocyanate.
Winter cress (Barbarea verna) seed preparations rich in phenethylisothiocyanate (PEITC) had strong in vivo and in vitro anti-inflammatory activity, significantly reducing the size of carrageenan-induced rat paw edema. This in vivo effect was comparable with that of the nonsteroidal anti-inflammatory drug aspirin. The seed preparation, in a concentration-dependent manner, reduced the mRNA levels of inflammation-related genes such as the inducible forms of cyclooxygenase and nitric-oxide synthase and the proinflammatory cytokine interleukin in lipopolysaccharide-stimulated mouse macrophage cell line RAW 264.7. Activity of the seed preparation was similar to that of the synthetic PEITC. PEITC was the most active of five different forms of isothiocyanate tested for their effects on in vitro proinflammatory gene expression. In vitro activity of the seed preparation was also compared with that of two known anti-inflammatory drugs. We conclude that Barbarea verna seed preparation may function as a potent anti-inflammatory agent, interfering with the transcription of proinflammatory genes.
Dey
Moul
M
Biotech Center, Rutgers University, New Brunswick, NJ 08901, USA.
Ribnicky
David
D
Kurmukov
Anvar G
AG
Raskin
Ilya
I
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D031216:Barbarea / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000188:drug therapy*; D015870:Gene Expression / Q000187:drug effects; D017879:Isothiocyanates / Q000302:isolation & purification / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D028321:Plant Preparations / Q000302:isolation & purification / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012639:Seeds / Q000737:chemistry
D000894:Anti-Inflammatory Agents, Non-Steroidal; D017879:Isothiocyanates; D028321:Plant Preparations; D012333:RNA, Messenger; C058305:phenethyl isothiocyanate