title: Granulocyte colony-stimulating factor treatment enhances the efficacy of cellular cardiomyoplasty with transplantation of embryonic stem cell-derived cardiomyocytes in infarcted myocardium.
abstract: We tested the hypothesis that granulocyte colony-stimulating factor (G-CSF) administration would enhance the efficacy of cellular cardiomyoplasty with embryonic stem (ES) cell-derived cardiomyocytes in infarcted myocardium. Three weeks after myocardial infarction by cryoinjury, Sprague-Dawley rats were randomized to receive either an injection of medium, ES cell-derived cardiomyocyte transplantation, G-CSF administration, or a combination of G-CSF administration and ES cell-derived cardiomyocyte transplantation. Eight weeks after treatment, the cardiac tissue formation, neovascularization, and apoptotic activity in the infarct regions were evaluated by histology and immunohistochemistry. The left ventricular (LV) dimensions and function of the treated heart were evaluated by echocardiography. Transplanted ES cell-derived cardiomyocytes survived and participated in the myocardial regeneration in the infarcted heart. A combination of G-CSF treatment and ES cell-derived cardiomyocyte transplantation significantly promoted angiogenesis and reduced the infarct area and cell apoptosis in the infarcted myocardium compared with ES cell-derived cardiomyocyte transplantation alone. The combination therapy also attenuated LV dilation, as compared with ES cell-derived cardiomyocyte transplantation alone. G-CSF treatment can enhance the efficacy of cellular cardiomyoplasty by ES cell-derived cardiomyocyte transplantation to treat myocardial infarction.
authors.affiliation: Department of Bioengineering, Hanyang University, Seoul 133-791, Republic of Korea.
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: So-Jung
authors.initials: SJ
authors.lastname: Gwak
authors.forename: Il-Kwon
authors.initials: IK
authors.lastname: Kim
authors.forename: Myeong-Chan
authors.initials: MC
authors.lastname: Cho
authors.forename: Kyung-Kuk
authors.initials: KK
authors.lastname: Hwang
authors.forename: Jin-Sook
authors.initials: JS
authors.lastname: Kwon
authors.forename: Cha Yong
authors.initials: CY
authors.lastname: Choi
authors.forename: Kyung Jong
authors.initials: KJ
authors.lastname: Yoo
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000818:Animals; D018421:Cardiomyoplasty; D002478:Cells, Cultured; D003131:Combined Modality Therapy; D004622:Embryo, Mammalian / Q000166:cytology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D051379:Mice; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000473:pathology / Q000601:surgery*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation
chemical_list: D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor