[Effects of bushen huoxue recipe on proliferation, differentiation and mineralization of osteoblasts in rats].
OBJECTIVE
To observe the effects of bushen huoxue recipe (BHR) on cell proliferation, differentiation, maturation and mineralization in osteoblasts of rats cultured in vitro.


METHODS
Osteoblasts from cranium of newborn SD rats were cultured by collagenase method. MTT, PNPP and ARS were used to observe the proliferation, activity of alkaline phosphatase (ALP) and the formation of mineral node of cultured osteoblasts effected by different concentrations of BHR at different time points (24, 48, 72 hrs after medication).


RESULTS
Different concentrations of BHR could enhance the cell proliferation rate and ALP activity, the effects of BHR in high and moderate concentration were more significant in enhancing the cell proliferation rate (P < 0.01). And the number of mineral node of cultured osteoblasts treated by high and moderate concentration BHR was much more than that of the untreated osteoblasts (P < 0.01).


CONCLUSIONS
BHR could promote the proliferation, differentiation, and mineralization of the osteoblasts.
Zhang
Hong-min
HM
Department of Orthopedics, Second Hospital of Xi' an Jiaotong University, Xi' an 710044. zhm197@163.com
Chen
Jun-chang
JC
Liu
Yi-heng
YH
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002113:Calcification, Physiologic / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D000469:Alkaline Phosphatase; D002118:Calcium