title: T-lymphocyte subset analysis using the automated hematology analyser CELL-DYN 4000 for patients with hematological disorders.
abstract: We evaluated the performance of a fully-automated hematology analyser CELL-DYN 4000 (CD4000; Abbott Laboratories, CA, USA) for T-lymphocyte subset analysis using the samples from 58 patients with hematological disorders. A flow cytometer (CytoronAbsolute; Ortho Diagnostics, NJ, USA) was used for comparison. Good correlations were shown between CD4000 and CytoronAbsolute for the of CD3(+), CD4(+), and CD8(+) T-lymphocyte counts (for each of correlations, r = 0.992, 0.992, and 0.969, respectively). This good correlation was sustained even in 24 cases in which the CD4(+) T-lymphocyte counts were less than 200/microl. In this group the correlation coefficients (r-values) for the comparisons between CD3(+), CD4(+), and CD8(+) T-lymphocytes measured by CD4000 and CytoronAbsolute were 0.991, 0.976, and 0.990, respectively. The CD4000 T-lymphocyte assay is a completely automated method, and does not require opening of the sample tube, which reduces exposure to any biohazards such as HIV. Furthermore, the method is rapid and requires only 7 min for completion of the analysis.
authors.affiliation: Clinical Hematology and Clinical Diagnostics, Graduate School of Medicine, Osaka City University, Osaka, Japan. yamane@msic.med.osaka-cu.ac.jp
authors.forename: Takahisa
authors.initials: T
authors.lastname: Yamane
authors.forename: Kiyoyuki
authors.initials: K
authors.lastname: Hagihara
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Nakamae
authors.forename: Taro
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hino
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology; D004867:Equipment Design; D005434:Flow Cytometry / Q000295:instrumentation*; D006402:Hematologic Diseases / Q000175:diagnosis* / Q000473:pathology; D006403:Hematologic Tests / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D018655:Lymphocyte Count; D012680:Sensitivity and Specificity; D016176:T-Lymphocyte Subsets / Q000473:pathology*