Rapid, noninvasive diagnosis of visceral leishmaniasis in India: comparison of two immunochromatographic strip tests for detection of anti-K39 antibody.
Used with blood or serum, a new anti-K39 antibody immunochromatographic strip test (IT-Leish; DiaMed AG) proved sensitive (range, 99 to 100%) and specific (range, 95 to 100%) for the noninvasive serodiagnosis of visceral leishmaniasis in India. Used with serum, the IT-Leish test and the existing Kalazar Detect test (InBios International, Inc.) yielded comparable results for symptomatic infection and identified apparent subclinical infection in 15 to 32% of healthy residents in a region where visceral leishmaniasis is highly endemic.
Sundar
Shyam
S
Kala-Azar Medical Research Center, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India. shyam_vns@sify.com
Maurya
Radheshyam
R
Singh
Rakesh K
RK
Bharti
K
K
Chakravarty
Jaya
J
Parekh
Ashish
A
Rai
Madhukar
M
Kumar
Kailash
K
Murray
Henry W
HW
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D007194:India; D007891:Leishmania / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis*; D010274:Parasitology / Q000379:methods; D015800:Protozoan Proteins / Q000276:immunology*; D011934:Reagent Strips; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D011934:Reagent Strips; C079253:K39 antigen, Leishmania