title: Characterization of an antibody that can detect the Kai1/CD82 murine metastasis suppressor.
abstract: BACKGROUND
  Kai1, also known as CD82, is a member of the tetraspanin family (TM4SF). The human homolog, KAI1, is an activation antigen of T-cells and is a metastasis suppressor for prostate and other cancers. Little is known about the mouse protein because of the lack of antibody reagents.
  
  
  METHODS
  Peptide immunized rabbits were used to generate polyclonal antibody to Kai1. The antibody was analyzed using immunoblotting, flow cytometry, and immunohistochemistry.
  
  
  RESULTS
  This antibody specifically recognizes murine Kai1 protein, crossreacts with rat Kai1 but not with human KAI1. The normal tissue distribution of this protein in mice is shown to be similar to that of the human homolog. Interestingly, mouse prostatic epithelium showed differential expression within the lobes.
  
  
  CONCLUSIONS
  This antibody, the first described that can specifically detect murine Kai1/CD82, should be very useful in addressing the mechanism of action of Kai1 in metastatic suppression.
authors.affiliation: Laboratory of Biosystems and Cancer, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. custema@mail.nih.gov
authors.forename: Mary C
authors.initials: MC
authors.lastname: Custer
authors.forename: John I
authors.initials: JI
authors.lastname: Risinger
authors.forename: Shelley
authors.initials: S
authors.lastname: Hoover
authors.forename: R Mark
authors.initials: RM
authors.lastname: Simpson
authors.forename: Tricia
authors.initials: T
authors.lastname: Patterson
authors.forename: J Carl
authors.initials: JC
authors.lastname: Barrett
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D045744:Cell Line, Tumor; D017931:DNA Primers; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D051861:Kangai-1 Protein / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D010446:Peptide Fragments / Q000276:immunology; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000276:immunology; D014162:Transfection
chemical_list: D000906:Antibodies; C497688:CD82 protein, human; D017931:DNA Primers; D051861:Kangai-1 Protein; D010446:Peptide Fragments