Comparative analysis of Trichinella spiralis and Trichinella nativa proteins by two-dimensional gel electrophoresis.
Trichinella spiralis and Trichinella nativa are both common wildlife parasites in Finland. However, they differ substantially in their resistance to below 0 degrees C temperatures in their natural hosts. T. nativa can live in frozen fox meat for years, whereas T. spiralis dies when frozen. In mouse muscle, the difference is not as evident; even T. nativa cannot maintain infectivity when kept at -20 degrees C for 1 week. Crude larval protein extracts of these two parasite species were analyzed by two-dimensional gel electrophoresis (2DE). The protein patterns showed clear differences, but matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) peptide mass fingerprint followed by database searches failed to identify these proteins, suggesting that they may still be uncharacterized. The patterns compared after freezing treatment at -20 degrees C revealed changes in the intensity of some protein spots. The antigenic differences of the species were analyzed with two-dimensional Western blots, which showed T. spiralis-specific proteins.
Näreaho
A
A
Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland. anu.nareaho@helsinki.fi
Ravanko
K
K
Hölttä
E
E
Sukura
A
A
D000818:Animals; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D015801:Helminth Proteins / Q000737:chemistry*; D007814:Larva / Q000737:chemistry; D051379:Mice / Q000469:parasitology; D010273:Parasitic Diseases, Animal / Q000469:parasitology; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D011817:Rabbits; D046031:Raccoon Dogs / Q000469:parasitology; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine / Q000469:parasitology; D014234:Trichinella / Q000737:chemistry*
D015801:Helminth Proteins; D010446:Peptide Fragments