Antinociceptive properties of coumarins, steroid and dihydrostyryl-2-pyrones from Polygala sabulosa (Polygalaceae) in mice.
We have investigated the possible antinociceptive action of the extract, fractions and pure compounds obtained from the whole plant Polygala sabulosa A. W. Bennett (Polygalaceae) in acetic acid-induced visceral pain in mice. Intraperitoneal injection of animals with the hydroalcoholic extract and fractions (CH(2)Cl(2), EtOAc, n-BuOH, aqueous fraction) (1-100 mg kg(-1)) caused a dose-related and significant inhibition of the acetic acid-induced visceral nociceptive response. The CH(2)Cl(2), EtOAc and n-BuOH fractions were more potent than the hydroalcoholic extract and aqueous fraction. The isolated compounds dihydrostyryl-2-pyrones (1, 2, 3), styryl-2-pyrone (7), alpha-spinasterol (9), scopoletin (10) and two esters of the coumarin (scopoletin) obtained semisynthetically, acetylscopoletin (10a) and benzoylscopoletin (10b) (0.001-10 mg kg(-1)), exhibited significant and dose-related antinociceptive effects against acetic acid-induced visceral pain. The results distinguished, for the first time, the extract, fractions and pure compounds obtained from P. sabulosa that produced marked antinociception against the acetic acid-induced visceral nociceptive response, supporting the ethnomedical use of P. sabulosa.
Meotti
Flavia Carla
FC
Departamento de Química, Universidade Federal de Santa Maria, 97110-000, Santa Maria, RS- Brazil.
Ardenghi
Juliana V
JV
Pretto
Juliana B
JB
Souza
Márcia M
MM
d' Avila Moura
Janaína
J
Junior
Anildo Cunha
AC
Soldi
Cristian
C
Pizzolatti
Moacir Geraldo
MG
Santos
Adair R S
AR
D015746:Abdominal Pain / Q000139:chemically induced / Q000188:drug therapy; D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D003374:Coumarins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010147:Pain Measurement / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D031801:Polygala / Q000737:chemistry*; D011753:Pyrones / Q000494:pharmacology; D013265:Stigmasterol / Q000031:analogs & derivatives* / Q000494:pharmacology
D000700:Analgesics; D003374:Coumarins; D010936:Plant Extracts; D011753:Pyrones; C031312:spinasterol; D013265:Stigmasterol; D019342:Acetic Acid