Study on the antifertility effects of the plasmid DNA vaccine expressing partial brLDH-C4'.
Partial cDNA sequence coding for Microtus brandti radde (Brandt's vole) testes-specific lactate dehydrogenase (brLDH-C4) was amplified by reverse transcription-polymerase chain reaction (RT-PCR). By inserting the product into the eukaryotic expression vector pCR3.1, pCR3.1-brLDH-C4' was obtained as the prototype of contraceptive DNA vaccine. Immunization with pCR3.1-brLDH-C4' in BALB/c mice generated antibodies specific to purified brLDH-C4' and native mouse LDH-C4 protein. The birth rate of the pCR3.1-brLDH-C4' immunized mice was found to be decreased significantly (80% lower than that of those immunized with pCR3.1). Functions of the elicited antibodies in sera from pCR3.1-brLDH-C4' inoculated mice were further explored. The results indicated that the antibodies from the mice injected with pCR3.1-brLDH-C4' could cause the agglutination of normal sperm suspension, while the ovarian structure and the development of ovarian follicles of these mice were not impaired, which gives a possible explanation for the immunocontraceptive effects of the pCR3.1-brLDH-C4' DNA vaccine.
Chang
Jian-Jun
JJ
State Key laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080, China.
Peng
Jing-Pian
JP
Yang
Ying
Y
Wang
Jing-Ling
JL
Xu
Li
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification / Q000494:pharmacology; D003411:Arvicolinae / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D019149:Bioreactors; D015153:Blotting, Western / Q000379:methods; D003269:Contraception, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli; D005260:Female; D005298:Fertility / Q000187:drug effects*; D005818:Genetic Engineering; D006367:HeLa Cells; D006801:Humans; D007114:Immunization; D007150:Immunohistochemistry / Q000379:methods; D007527:Isoenzymes / Q000235:genetics / Q000276:immunology; D007770:L-Lactate Dehydrogenase / Q000235:genetics* / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010957:Plasmids; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D016415:Sequence Alignment; D013073:Sperm Agglutination / Q000187:drug effects; D014162:Transfection / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D007770:L-Lactate Dehydrogenase; C409673:lactate dehydrogenase C4