Optically sectioned fluorescence lifetime imaging using a Nipkow disk microscope and a tunable ultrafast continuum excitation source.
We demonstrate an optically sectioned fluorescence lifetime imaging microscope with a wide-field detector, using a convenient, continuously tunable (435-1150 nm) ultrafast source for fluorescence imaging applications that is derived from a visible supercontinuum generated in a microstructured fiber.
Grant
D M
DM
Physics Department, Imperial College London, Prince Consort Road, London SW7 2BW, UK.
Elson
D S
DS
Schimpf
D
D
Dunsby
C
C
Requejo-Isidro
J
J
Auksorius
E
E
Munro
I
I
Neil
M A A
MA
French
P M W
PM
Nye
E
E
Stamp
G
G
Courtney
P
P