title: Inhibitory effect of Thai plant extracts on P-glycoprotein mediated efflux.
abstract: Curcuminoids from Curcuma longa L. and extracts of Psidium guajava L., Andrographis paniculata (Burm. f.) Nees, Phyllanthus emblica L. and Solanum trilobatum L. were investigated for their inhibitory effect on P-glycoprotein (P-gp) on the efflux transport of rhodamine 123 (Rho-123 ) in Caco-2 cells and rat ileum. Of the five tested samples, curcuminoids and an extract of P. guajava showed the highest inhibitory effect on P-gp mediated efflux of Rho-123 in Caco-2 cells. Additionally, they were found to have equal potential in inhibiting Rho-123 efflux transport from serosal to mucosal surfaces of the rat ileum.
authors.affiliation: Department of Pharmacy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayutthaya, Rajathavee, Bangkok 10400, Thailand. pyvbp@mahidol.ac.th
authors.forename: Varaporn Buraphacheep
authors.initials: VB
authors.lastname: Junyaprasert
authors.forename: Noppamas
authors.initials: N
authors.lastname: Soonthornchareonnon
authors.forename: Suchitra
authors.initials: S
authors.lastname: Thongpraditchote
authors.forename: Teruo
authors.initials: T
authors.lastname: Murakami
authors.forename: Mikihisa
authors.initials: M
authors.lastname: Takano
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000187:drug effects / Q000378:metabolism*; D035685:Andrographis / Q000737:chemistry; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D018938:Caco-2 Cells; D030024:Curcuma / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D008297:Male; D026901:Membrane Transport Proteins / Q000187:drug effects*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D029842:Phyllanthus emblica / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D059808:Polyphenols; D031647:Psidium / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D020112:Rhodamine 123 / Q000378:metabolism; D032322:Solanum / Q000737:chemistry; D013785:Thailand
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D005419:Flavonoids; D026901:Membrane Transport Proteins; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D020112:Rhodamine 123