[Study on the extraction and purification for total flavonoids in Lonicera japonica Thunb. and Chrysanthemum morifolium Tzvel].
OBJECTIVE
To optimize the extraction and purification conditions for total flavonoids in Lonicera japonica Thunb. and Chrysanthemum morifolium Tzvel.


METHODS
Comparision of several extraction processes, the L9 (3(4)) orthogonal design and macroreticular absorption resin D101 purification technique were performed.


RESULTS
The optimum extraction conditions were 8 volume of 55% ethanol, 1 h refluence at 100 degrees C , water bath for 2 times. The extracted fluid was absorbed with the resin D101. The elutions were water, 30% enthanol, 70% enthanol. The obtained elution of 70% enthanol was evaporated and the content of total flavonoids was 62. 7%.


CONCLUSIONS
The method is simple, approved and fit for industrial production.
Yang
Jun
J
Jinling Pharmaceutical Co. Ltd, Nanjing, Jiangsu.
Zhang
Xiaojing
X
Tian
Lijuan
L
Wang
Xingwang
X
D000704:Analysis of Variance; D031202:Chrysanthemum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D035264:Flowers / Q000737:chemistry; D031239:Lonicera / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D014867:Water
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D014867:Water; D000431:Ethanol