title: Effect of sodium fluoride on the expression of bcl-2 family and osteopontin in rat renal tubular cells.
abstract: Our earlier studies showed that the apoptosis of renal tubules can be induced by sodium fluoride (NaF). The present study was designed to estimated the effects of B-cell lymphoma/leukemia 2 (Bcl-2), Bcl-2-associated protein X (Bax), and osteopontin (OPN) on the apoptosis of renal tubular cells induced by NaF at different levels. The technique of reverse transcription-polymerase chain reaction and densitometer scanning volume density were used to evaluate the changes of Bcl-2, Bax, and OPN mRNA in tubular cells treated with different doses of NaF (0, 1, 5, 7.5, 12.5 mgF-/L) for 48 h. Compared to control, the level of Bax mRNA significantly increased at cells of the 7.5- and 12.5-mg F-/L groups and the expression of Bcl-2 mRNA obviously decreased at cells of the 5- and 7.5-mg F-/L groups. The NaF also enhanced the expression of OPN mRNA in a dose-dependent manner, but the strongest expression of OPN mRNA was observed at cells of the 7.5-mg F-/L group. The results suggested that NaF induces the apoptosis in renal tubules via activation of the Bax expression and Bcl-2 suppression; OPN probably acts as protective role against apoptosis in fluoride-treated renal cells.
authors.affiliation: Institute of Endemic Diseases, Jilin University, 828 Xinmin, ChangChun 130021, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Xiang-Qun
authors.initials: XQ
authors.lastname: Jin
authors.forename: Ling
authors.initials: L
authors.lastname: Jing
authors.forename: Guang-Sheng
authors.initials: GS
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D053495:Osteopontin; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000235:genetics; D012969:Sodium Fluoride / Q000633:toxicity*; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D051028:bcl-2-Associated X Protein; D053495:Osteopontin; D012969:Sodium Fluoride