title: Electrodes modified with the phase transition polymer and heme peptide: biocatalysis and biosensing with tunable activity and dynamic range.
abstract: An electrode was modified with a phase transition polymer, poly(N-isopropylacrylamide), and the polymer was further modified with a peroxidase model compound, heme peptide (HP). As the polymer layer shrank at temperatures above 30-40 degrees C, the catalytic activity of the HP molecules for H(2)O(2) reduction improved, and simultaneously, the number of HP molecules that can communicate electrochemically with the electrode increased. As a result, the catalytic current for H(2)O(2) reduction in the shrunken state was 4 times larger than that in the swollen state. This reversible change was exploited for tuning the sensitivity and dynamic range of the HP electrode in H(2)O(2) biosensing. The dynamic range in inhibition-based biosensing of imidazole derivatives was also tunable.
authors.affiliation: Institute of Industrial Science, University of Tokyo, Meguro-ku, Tokyo 153-8505, Japan.
authors.forename: Kikuo
authors.initials: K
authors.lastname: Komori
authors.forename: Kazutake
authors.initials: K
authors.lastname: Takada
authors.forename: Tetsu
authors.initials: T
authors.lastname: Tatsuma
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D015374:Biosensing Techniques; D002384:Catalysis; D004566:Electrodes; D006420:Hemeproteins / Q000037:antagonists & inhibitors / Q000737:chemistry*; D066298:In Vitro Techniques; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D000180:Acrylic Resins; D006420:Hemeproteins; D010446:Peptide Fragments; C052970:poly-N-isopropylacrylamide