Intein-mediated in vitro synthesis of lipidated Ras proteins.
Fully functional lipid-modified Ras proteins suitable for the study of Ras-membrane interactions and embodying exclusively native amide bonds can be synthesized in preparative amounts by means of Expressed Protein Ligation.
Gottlieb
D
D
Max Planck Institute of Molecular Physiology, Otto-Hahn-Str. 11, 44227, Dortmund, Germany.
Grunwald
C
C
Nowak
C
C
Kuhlmann
J
J
Waldmann
H
H
D000577:Amides / Q000737:chemistry; D001665:Binding Sites; D047668:Inteins / Q000502:physiology*; D008055:Lipids / Q000737:chemistry*; D008074:Lipoproteins / Q000138:chemical synthesis*; D008565:Membrane Proteins / Q000737:chemistry; D008956:Models, Chemical; D015202:Protein Engineering / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D018631:ras Proteins / Q000737:chemistry*
D000577:Amides; D008055:Lipids; D008074:Lipoproteins; D008565:Membrane Proteins; D018631:ras Proteins