Influence of orally administered CpG-ODNs on the humoral response to bovine serum albumin (BSA) in chickens.
Synthetic oligodeoxynucleotides containing CpG motifs (CpG-ODNs) have been reported to be effective mucosal adjuvants in mice when given orally. Studies on their effectiveness in chickens are currently very limited. This study investigated whether CpG-ODNs could adjuvant the immune response to BSA when given orally to a commercial line of SCWL chickens. In two experiments, performed over time, chickens were given selected concentrations of CpG-ODNs with BSA followed by 6 consecutive days of ad libitum access to drinking water containing 1.4 mg/ml BSA. Serum responses, and in some cases intestinal specific antibodies, were measured out to 33 days post-immunization. Birds receiving a single dose of CpG-ODN had consistently higher IgG, IgM, and IgA titers in the serum, dependent upon dose, and in specific areas of the intestine when compared to the non-immunized and BSA only groups. These findings suggest that a single oral CpG-ODN administration can accelerate the kinetics of antigen specific antibodies of all three isotypes in commercial-strain chickens immunized via the drinking water using common protein antigen.
Ameiss
K A
KA
Poultry Health Research Laboratory, Texas A&M University, College Station, TX 77843-2472, USA.
El Attrache
J
J
Barri
A
A
McElroy
A P
AP
Caldwell
D J
DJ
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D000284:Administration, Oral; D000818:Animals; D000906:Antibodies / Q000187:drug effects* / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007422:Intestines / Q000187:drug effects / Q000276:immunology; D008297:Male; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage* / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000276:immunology*; D013997:Time Factors
D000276:Adjuvants, Immunologic; D000906:Antibodies; C408982:CPG-oligonucleotide; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009838:Oligodeoxyribonucleotides; D012710:Serum Albumin, Bovine