CD 14+ cell collection in non-cytokine-stimulated donors with the COM.TEC cell separator.
BACKGROUND
The COM.TEC cell separator (Fresenius Hemocare), equipped with the MNC program (single-stage chamber) and the PBSC-LYM program (dual-stage chamber), was evaluated for CD 14+ cell collection regarding cell yields, collection efficiencies (CEs), and the content of residual cells in harvests.


METHODS
Twenty-four non-cytokine-stimulated donors underwent 5-L mononuclear cell (MNC) collections on the COM.TEC device to compare both programs. Two software versions (v02.03.05 vs. v 03.00.04) were investigated for optimization of the CD 14+ cell collection process. Blood counts of donors and products were analyzed for CD 14+ cells by flow cytometry and for platelets (PLTs), white blood cells, and red blood cells (RBCs) by a blood cell counter.


RESULTS
In 5-L collections, the MNC program resulted in high CEs (83+/- 23%) and yields (1.2 x 10(9)+/- 0.6 x 10(9) per unit) of CD 14+ cells, but the products showed high residual PLTs. The use of a dual-stage chamber in the PBSC-LYM program produced a low content of residual PLTs (0.7 x 10(11) +/- 0.3 x 10(11) per unit) and RBCs but failed to reach a target of 1 x 10(9) CD 14+ cells. Modulated light to stabilize the buffy-coat detection by the interface monitor significantly improved CD 14+ cell enrichment. By use of the PBSC-LYM program, higher centrifuge velocity (1700 rpm [382 x g] vs. 1500 rpm [297 x g]) improved significantly CD 14+ cell yields (0.7 x 10(9) vs. 0.5 x 10(9) cells).


CONCLUSIONS
A pure CD 14+ cell product with low numbers of residual cells was obtained by the PBSC-LYM program, which could be useful for good manufacturing practice-conformed production within closed systems. The MNC program offers the collection of high CD 14+ cell yields with excellent CEs but also high residual PLT counts.
Strasser
Erwin F
EF
Department of Transfusion Medicine and Hemostaseology, University Hospital, FAU Erlangen-Nürnberg, Germany. erwin.strasser@tras.imed.uni-erlangen.de
Hendelmeier
Martin
M
Weisbach
Volker
V
Zimmermann
Robert
R
Ringwald
Jürgen
J
Juntke
Ronald
R
Sauer
Gudrun
G
Zingsem
Jürgen
J
Eckstein
Reinhold
R
D001782:Blood Donors; D005260:Female; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation / Q000379:methods; D007963:Leukocytes, Mononuclear; D018950:Lipopolysaccharide Receptors; D008297:Male; D012984:Software
D018950:Lipopolysaccharide Receptors