title: Two new methods monitoring kinetics of hydrolysis of acetylcholine and acetylthiocholine.
abstract: Hydroxylamine and HPLC methods, measuring in vitro kinetics of enzymatic hydrolysis of acetylcholine or acetylthiocholine by cholinesterases, are described. The hydroxylamine method determines the dependence of substrate concentration vs. time, the HPLC method is able to measure simultaneously the time dependences of substrate and both primary products, choline or thiocholine, and acetic acid. Practical determinations are shown, comparison with known (above all Ellman's and pH-stat) methods, advantages and disadvantages are discussed.
authors.affiliation: Faculty of Chemical Technology, University of Pardubice, Department of Physical Chemistry, Czech Republic.
authors.forename: Sarka
authors.initials: S
authors.lastname: Stepánková
authors.forename: Martina
authors.initials: M
authors.lastname: Vrańová
authors.forename: Pavla
authors.initials: P
authors.lastname: Zdrazilová
authors.forename: Karel
authors.initials: K
authors.lastname: Komers
authors.forename: Alena
authors.initials: A
authors.lastname: Komersová
authors.forename: Alexander
authors.initials: A
authors.lastname: Cegan
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000122:Acetylthiocholine / Q000378:metabolism*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D016903:Drug Monitoring / Q000379:methods; D006898:Hydroxylamines; D007202:Indicators and Reagents; D007700:Kinetics
chemical_list: D006898:Hydroxylamines; D007202:Indicators and Reagents; D000122:Acetylthiocholine; D000109:Acetylcholine