title: Peroxisome proliferation in liver of rats fed oxidized frying oil.
abstract: Oxidized frying oil (OFO) activates peroxisome proliferator-activated receptor a (PPAR alpha) in vitro and in vivo. As most PPARalpha activators are also peroxisome proliferators (PP), this study was aimed at exploring whether OFO induces peroxisome proliferation in the liver of rats. Four groups of male weanling Sprague-Dawley rats were fed the following diets for 6 wk: a basal diet containing 5 g/100 g fresh soybean oil (LSB), high-fat diets containing 20 g/100 g of fresh soybean oil (HSB as a control). OFO (HO) or fish oil (HF, as a positive control). Hepatomegaly and peroxisome proliferation in the liver of the HO group of rats were higher than those of the HF group. In addition, the acyl-CoA oxidase (ACO) activity, as well as cytochrome P450 4A (CYP4A) protein content in the livers of the HO group were 6 fold those of the HSB group, but were 2.5 fold in those of the HF group. These results indicated that dietary OFO induced typical responses to PPARalpha signaling. Moreover. as a dietary source, the OFO prepared under our frying conditions appears to be a more potent peroxisome proliferator than fish oil.
authors.affiliation: Department of Nutrition, China Medical University, Taichung, 404, Taiwan.
authors.forename: Pei-Min
authors.initials: PM
authors.lastname: Chao
authors.forename: Mei-Fang
authors.initials: MF
authors.lastname: Yang
authors.forename: Yu-Na
authors.initials: YN
authors.lastname: Tseng
authors.forename: Ko-Ming
authors.initials: KM
authors.lastname: Chang
authors.forename: Kuo-Shyan
authors.initials: KS
authors.lastname: Lu
authors.forename: Ching-Jang
authors.initials: CJ
authors.lastname: Huang
mesh_terms: D042962:Acyl-CoA Oxidase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D003296:Cooking; D042926:Cytochrome P-450 CYP4A / Q000235:genetics / Q000378:metabolism; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology; D015870:Gene Expression; D006651:Histocytochemistry; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D010084:Oxidation-Reduction; D047493:PPAR alpha / Q000378:metabolism; D020025:Peroxisome Proliferators / Q000494:pharmacology*; D020675:Peroxisomes / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013024:Soybean Oil / Q000494:pharmacology
chemical_list: D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D047493:PPAR alpha; D020025:Peroxisome Proliferators; D013024:Soybean Oil; D042926:Cytochrome P-450 CYP4A; D042962:Acyl-CoA Oxidase