Development of an improved heated interface for coupling solid-phase microextraction to high-performance liquid chromatography.
The aim of the study described in this report has been the development and the evaluation of a new improved interface to be operated under continuous heating, for on-line coupling solid-phase microextraction (SPME) to high-performance liquid chromatography (HPLC). Heating is desirable to increase desorption rate and decrease carryover. The results obtained have been compared with that obtained by off-line desorption and online desorption without heating. The SPME-HPLC interface described here has an inner volume of 60 microL, fixation for infinite points and a novel leak less sealing system. When the heating system was used, the area values were almost 10-fold higher than that obtained using the off-line mode. The obtained chromatograms showed an increasing of the area and height of chromatographic peaks and proved the excellent performance and reproducibility of the interface developed in this work.
Rodrigues
José Carlos
JC
University of São Paulo, Institute of Chemistry at São Carlos, 13566-590 São Carlos, SP, Brazil.
Neto
Alvaro J Santos
AJ
Fernandes
Christian
C
Alves
Claudete
C
Contadori
Alex S
AS
Lanças
Fernando M
FM
D005591:Chemical Fractionation / Q000295:instrumentation* / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D005473:Fluoxetine / Q000097:blood / Q000302:isolation & purification*; D006358:Hot Temperature; D015203:Reproducibility of Results
D005473:Fluoxetine