Quantitative proteomic analysis of drug-induced changes in mycobacteria.
A new approach for qualitative and quantitative proteomic analysis using capillary liquid chromatography and mass spectrometry to study the protein expression response in mycobacteria following isoniazid treatment is discussed. In keeping with known effects on the fatty acid synthase II pathway, proteins encoded by the kas operon (AcpM, KasA, KasB, Accd6) were significantly overexpressed, as were those involved in iron metabolism and cell division suggesting a complex interplay of metabolic events leading to cell death.
Hughes
Minerva A
MA
Department of Chemistry, The Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA.
Silva
Jeffrey C
JC
Geromanos
Scott J
SJ
Townsend
Craig A
CA
D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000032:analysis; D002473:Cell Wall / Q000378:metabolism; D002853:Chromatography, Liquid; D007538:Isoniazid / Q000494:pharmacology*; D050356:Lipid Metabolism; D013058:Mass Spectrometry; D009163:Mycobacterium bovis / Q000187:drug effects* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000187:drug effects; D040901:Proteomics / Q000379:methods*
D000995:Antitubercular Agents; D001426:Bacterial Proteins; D020543:Proteome; D007538:Isoniazid