title: Voltammetric behavior of the transfer of mono- and polyammonium ions across a phospholipid monolayer at the nitrobenzene/water interface.
abstract: The influence of a phospholipid, dipalmitoyl phosphatidylcholine, layer at a nitrobenzenelwater interface on the transfer of tetraethylammonium ion and a polyammonium anti-fungus agent, poly[(dimethylimino)(2-oxo-1,2-ethanediyl)imino1,6-hexanediylimino (1-oxo-1,2-ethanediyl)(dimethylimino)-1,6-hexanediyl] ion, across the interface was studied by normal pulse voltammetry. When the phospholipid was adsorbed to form a monolayer at the nitrobenzenelwater interface by its addition to the organic phase, the half-wave potential in the current vs. potential curves for the transfer of tetraethylammonium ion did not change, but the limiting current was significantly decreased at certain sampling times, indicating a retarding effect of the layer on the ion-transfer. On the other hand, in the current vs. potential curves for the transfer of the polyammonium ion, no significant change in either the half-wave potential or the limiting current was observed upon adding the phospholipid, indicating that the polyammonium ion can easily permeate through the phospholipid layer. The results suggest a new application of the voltammetric technique to the study of cell membrane permeability to polyionic bioactive compounds.
authors.affiliation: Department of Bioscience, Fukui Prefectural University, Matsuoka, Fukui 910-1195, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Katano
authors.forename: Yuji
authors.initials: Y
authors.lastname: Murayama
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Tatsumi
authors.forename: Takao
authors.initials: T
authors.lastname: Hibi
authors.forename: Tokuji
authors.initials: T
authors.lastname: Ikeda
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kameoka
authors.forename: Toshihide
authors.initials: T
authors.lastname: Tsukatani