Elasticity and adhesion of resting and lipopolysaccharide-stimulated macrophages.
Colloidal Force Microscopy was employed to study the viscoelastic and adhesive properties of macrophages upon stimulation with lipopolysaccharide (LPS). Force vs. distance measurements were performed. The adhesion of LPS-stimulated cells (separation force=37+/-3 nN) was almost twice as high as that of resting macrophages (16+/-1 nN). Upon retraction pulling of membrane tethers was observed. Tether lengths and forces at which rupture take place did not depend on stimulation. The reduced Young's modulus K, a measure of cytoskeleton elasticity, was three times lower than that of the control. The data show that LPS has profound effects on cytomechanical and adhesion properties of macrophages.
Leporatti
Stefano
S
Institute of Medical Physics and Biophysics, University of Leipzig, D-04107 Leipzig, Saxony, Germany. leps@medizin.uni-leipzig.de
Gerth
Anja
A
Köhler
Guido
G
Kohlstrunk
Bernd
B
Hauschildt
Sunna
S
Donath
Edwin
E
D002448:Cell Adhesion / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism; D004548:Elasticity; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D020527:Microscopy, Scanning Probe / Q000379:methods; D013314:Stress, Mechanical
D008070:Lipopolysaccharides