title: Antinociceptive properties of coumarins, steroid and dihydrostyryl-2-pyrones from Polygala sabulosa (Polygalaceae) in mice.
abstract: We have investigated the possible antinociceptive action of the extract, fractions and pure compounds obtained from the whole plant Polygala sabulosa A. W. Bennett (Polygalaceae) in acetic acid-induced visceral pain in mice. Intraperitoneal injection of animals with the hydroalcoholic extract and fractions (CH(2)Cl(2), EtOAc, n-BuOH, aqueous fraction) (1-100 mg kg(-1)) caused a dose-related and significant inhibition of the acetic acid-induced visceral nociceptive response. The CH(2)Cl(2), EtOAc and n-BuOH fractions were more potent than the hydroalcoholic extract and aqueous fraction. The isolated compounds dihydrostyryl-2-pyrones (1, 2, 3), styryl-2-pyrone (7), alpha-spinasterol (9), scopoletin (10) and two esters of the coumarin (scopoletin) obtained semisynthetically, acetylscopoletin (10a) and benzoylscopoletin (10b) (0.001-10 mg kg(-1)), exhibited significant and dose-related antinociceptive effects against acetic acid-induced visceral pain. The results distinguished, for the first time, the extract, fractions and pure compounds obtained from P. sabulosa that produced marked antinociception against the acetic acid-induced visceral nociceptive response, supporting the ethnomedical use of P. sabulosa.
authors.affiliation: Departamento de Química, Universidade Federal de Santa Maria, 97110-000, Santa Maria, RS- Brazil.
authors.forename: Flavia Carla
authors.initials: FC
authors.lastname: Meotti
authors.forename: Juliana V
authors.initials: JV
authors.lastname: Ardenghi
authors.forename: Juliana B
authors.initials: JB
authors.lastname: Pretto
authors.forename: Márcia M
authors.initials: MM
authors.lastname: Souza
authors.forename: Janaína
authors.initials: J
authors.lastname: d' Avila Moura
authors.forename: Anildo Cunha
authors.initials: AC
authors.lastname: Junior
authors.forename: Cristian
authors.initials: C
authors.lastname: Soldi
authors.forename: Moacir Geraldo
authors.initials: MG
authors.lastname: Pizzolatti
authors.forename: Adair R S
authors.initials: AR
authors.lastname: Santos
mesh_terms: D015746:Abdominal Pain / Q000139:chemically induced / Q000188:drug therapy; D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D003374:Coumarins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010147:Pain Measurement / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D031801:Polygala / Q000737:chemistry*; D011753:Pyrones / Q000494:pharmacology; D013265:Stigmasterol / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000700:Analgesics; D003374:Coumarins; D010936:Plant Extracts; D011753:Pyrones; C031312:spinasterol; D013265:Stigmasterol; D019342:Acetic Acid