The Bifidobacterium longum NCIMB 702259T ctr gene codes for a novel cholate transporter.
Preexposure of Bifidobacterium longum NCIMB 702259T to cholate caused increased resistance to cholate, chloramphenicol, and erythromycin. The B. longum ctr gene, encoding a cholate efflux transporter, was transformed into the efflux-negative mutant Escherichia coli KAM3, conferring resistance to bile salts and other antimicrobial compounds and causing the efflux of [14C]cholate.
Price
Claire E
CE
Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, 7701 Cape Town, South Africa.
Reid
Sharon J
SJ
Driessen
Arnold J M
AJ
Abratt
Valerie R
VR
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001644:Bifidobacterium / Q000187:drug effects* / Q000235:genetics; D001647:Bile Acids and Salts / Q000494:pharmacology; D002250:Carbon Radioisotopes / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D020355:Cholates / Q000378:metabolism* / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001647:Bile Acids and Salts; D002250:Carbon Radioisotopes; D020355:Cholates; D026901:Membrane Transport Proteins; D004917:Erythromycin; D002701:Chloramphenicol