title: Derivation of human embryonic stem cells in defined conditions.
abstract: We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
authors.affiliation: WiCell Research Institute, PO Box 7365, Madison, Wisconsin 53707-7365, USA.
authors.forename: Tenneille E
authors.initials: TE
authors.lastname: Ludwig
authors.forename: Mark E
authors.initials: ME
authors.lastname: Levenstein
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Jones
authors.forename: W Travis
authors.initials: WT
authors.lastname: Berggren
authors.forename: Erika R
authors.initials: ER
authors.lastname: Mitchen
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Frane
authors.forename: Leann J
authors.initials: LJ
authors.lastname: Crandall
authors.forename: Christine A
authors.initials: CA
authors.lastname: Daigh
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Conard
authors.forename: Marian S
authors.initials: MS
authors.lastname: Piekarczyk
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Llanas
authors.forename: James A
authors.initials: JA
authors.lastname: Thomson
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D003470:Culture Media