title: Bovine leukemia virus high tax molecular clone experimentally induces leukemia/lymphoma in sheep.
abstract: Sheep were inoculated with high tax coded pBLV-IF (H group, Nos.1-5) of bovine leukemia virus (BLV), wild tax coded pBLV-IF (W group, Nos. 6-11), or control plasmid (C group, Nos. 12-14). During the observation period (4 to 46 months), 5 of 5 cases in H group and 3 of 6 cases (Nos. 6, 7, 9) in W group became positive for gp 51. Only 1 case in H group became leukemic, and one case each of H and W groups developed lymphoma. In No. 3, lesions were found in multiple organs including the lymph nodes, gastrointestinal tract following abomasum, and heart. In No. 6, lesions of lymphoma were found only in the jejunum and heart. Morphologically, small to middle-sized lymphocytic neoplastic (NP) cells were found in both cases, but lymphoblastic NP cells were found only in No. 3. By immunohistochemical examination, the phenotypes of NP cells were determined as CD1-, CD4-, CD5- -, CD8alpha-, sIgM+, lambda light chain+, B-B4+, MHC class II+ in both case. The results of this study indicate that inoculation of pBLV-IF can induce lymphocytic and lymphoblastic leukemia/lymphoma in sheep. Additionally, it is suggested that the expression rate of tax gene is not associated with the development of leukemia/lymphoma in sheep experimentally inoculated with pBLV-IF.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Iwate, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Okada
authors.forename: Norihiro
authors.initials: N
authors.lastname: Nakae
authors.forename: Konomi
authors.initials: K
authors.lastname: Kuramochi
authors.forename: Shan-ai
authors.initials: SA
authors.lastname: Yin
authors.forename: Manabu
authors.initials: M
authors.lastname: Ikeda
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Takami
authors.forename: Tou-ichi
authors.initials: T
authors.lastname: Hirata
authors.forename: Masanobu
authors.initials: M
authors.lastname: Goryo
authors.forename: Shigeru
authors.initials: S
authors.lastname: Numakunai
authors.forename: Shin-nosuke
authors.initials: SN
authors.lastname: Takeshima
authors.forename: Masahiko
authors.initials: M
authors.lastname: Takahashi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tajima
authors.forename: Satoru
authors.initials: S
authors.lastname: Konnai
authors.forename: Misao
authors.initials: M
authors.lastname: Onuma
authors.forename: Yoko
authors.initials: Y
authors.lastname: Aida
mesh_terms: D000818:Animals; D041981:Gastrointestinal Tract / Q000473:pathology; D016356:Gene Products, tax / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D007150:Immunohistochemistry / Q000662:veterinary; D016130:Immunophenotyping / Q000662:veterinary; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000378:metabolism*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D008198:Lymph Nodes / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D012756:Sheep; D012757:Sheep Diseases / Q000473:pathology* / Q000821:virology*
chemical_list: D016356:Gene Products, tax; D006023:Glycoproteins; C119449:glycoprotein GP51