title: Characterisation of a DM domain-containing transcription factor from Trichostrongylus vitrinus (Nematoda: Strongylida).
abstract: The sequence and expression profile of Tv-mab-23, a gene encoding a DM domain-containing protein from the parasitic nematode Trichostrongylus vitrinus, were investigated. The gene, containing an open reading frame (ORF) of 537 bp, encoded a predicted protein which had an overall amino acid identity of 45% to the MAB-23 molecule of the free-living nematode Caenorhabditis elegans. High levels of conservation were particularly predominant at the amino terminus of the proteins, with 86% identity over the first 58 amino acid residues in a region containing the highly conserved zinc module of the DM domain and conserved regions of the recognition helix of DM domain-containing transcription factors. Tv-mab-23 was expressed in a stage-specific manner in T. vitrinus. The highest levels of expression in fourth stage larvae coincide with the period in which profound gender-specific alterations in physiology occur in both the parasitic and free-living nematodes.
authors.affiliation: Parasitology Division, Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, Scotland, United Kingdom. nisba@mri.sari.ac.uk
authors.forename: Alasdair J
authors.initials: AJ
authors.lastname: Nisbet
authors.forename: Peter
authors.initials: P
authors.lastname: Geldhof
authors.forename: Mark
authors.initials: M
authors.lastname: Pellegrino
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology; D018509:DNA, Helminth / Q000737:chemistry; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012727:Sex Characteristics; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014254:Trichostrongylus / Q000235:genetics* / Q000502:physiology*; D016335:Zinc Fingers
chemical_list: D018509:DNA, Helminth; D014157:Transcription Factors