title: Cross-reactivity between IgE-binding proteins from Anisakis simplex and Dermatophagoides pteronyssinus.
abstract: An association was found between Anisakis simplex (As) and Dermatophagoides pteronyssinus (Dp) sensitization. One recent study shows a cross-reactivity between As and Dp and tropomyosin (tr)is suspected as being one of the proteins responsible of this cross-reaction. The aim of our study was: 1) to confirm the cross-reactivity between Dp and As; 2) to determine the importance of tr in this cross reaction. SDS-PAGE analysis of Dp and As (metabolic and somatic) extracts was carried out. Then an IgE immunoblotting test using serum from a patient who had specific IgE only to Dp and As and immunoblotting inhibition experiments using Dp extract and tr as inhibitors were performed. We found that patients serum reacted: 1) against larval As antigens with a molecular weight (mw) of 25 kilodalton (kD) and a mw > than 100 kD, 2) against various metabolic As antigens with a mw > than 100 kD, a mw ranging approximately from 35 to 50 kD, and a mw around 20 kD, and 3) against Dp proteins with mw between 35 and 55 kD. Preincubation of patient's serum with Dp extract caused the disappearance of reactivity against antigens with a mw > than 100 kD in both larval and metabolic As extracts and against proteins with mw ranging approximately from 35 to 50 kD in the metabolic As extract. Preincubation of patients serum with As extract caused the disappearance of reactivity against antigens with mw between 35 and 55 kD in the Dp extract. Pre-incubation of patients serum with tr did not induce any change in the immunoblotting profile. The results show that 1) cross-reactive components between Dp and As are some proteins with a mw ranging approximately from 35 to 50 kD and with a mw > than 100 kD, and 2) tr is not involved in cross-reactivity between As and Dp.
authors.affiliation: Pediatric Allergy and Pulmonary Center, Anna Meyer Children's Hospital, Department of Pediatrics, University of Florence, Italy. r.bernardini@meyer.it
authors.forename: R
authors.initials: R
authors.lastname: Bernardini
authors.forename: G
authors.initials: G
authors.lastname: Mistrello
authors.forename: E
authors.initials: E
authors.lastname: Novembre
authors.forename: D
authors.initials: D
authors.lastname: Roncarolo
authors.forename: S
authors.initials: S
authors.lastname: Zanotta
authors.forename: E
authors.initials: E
authors.lastname: Lombardi
authors.forename: A
authors.initials: A
authors.lastname: Cianferoni
authors.forename: N
authors.initials: N
authors.lastname: Pucci
authors.forename: M
authors.initials: M
authors.lastname: De Martino
authors.forename: A
authors.initials: A
authors.lastname: Vierucci
mesh_terms: D000328:Adult; D000485:Allergens / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D000818:Animals; D017163:Anisakis / Q000737:chemistry / Q000378:metabolism*; D000918:Antibody Specificity; D001249:Asthma / Q000276:immunology; D002648:Child; D003429:Cross Reactions; D040002:Dermatophagoides pteronyssinus / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D037502:Galectin 3 / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000032:analysis / Q000737:chemistry; D007814:Larva / Q000737:chemistry / Q000276:immunology; D008970:Molecular Weight
chemical_list: D000485:Allergens; D037502:Galectin 3; D007073:Immunoglobulin E