title: Detection of recombinant epoetin and darbepoetin alpha after subcutaneous administration in the horse.
abstract: A direct detection method for anti-doping control of recombinant human erythropoietin (rHuEPO) abuse in racehorses is proposed. This method involves screening of plasma (or serum) by an enzyme-linked immunosorbent assay specific for human EPO and confirmation in urine samples by characterization of the urinary EPO isoelectric profile. This method was tested on horses that were administered epoetin alpha (rHuEPO) and the hyper-glycosylated form of this drug (darbepoetin alpha).
authors.affiliation: Laboratoire National de Dépistage du Dopage, Châtenay-Malabry, France. f.lasne@lndd.com
authors.forename: Françoise
authors.initials: F
authors.lastname: Lasne
authors.forename: Marie-Agnes
authors.initials: MA
authors.lastname: Popot
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Varlet-Marie
authors.forename: Laurent
authors.initials: L
authors.lastname: Martin
authors.forename: Jean-Antoine
authors.initials: JA
authors.lastname: Martin
authors.forename: Yves
authors.initials: Y
authors.lastname: Bonnaire
authors.forename: Michel
authors.initials: M
authors.lastname: Audran
authors.forename: Jacques
authors.initials: J
authors.lastname: de Ceaurriz
mesh_terms: D000818:Animals; D000068256:Darbepoetin alfa; D004797:Enzyme-Linked Immunosorbent Assay; D000068817:Epoetin Alfa; D004921:Erythropoietin / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine; D006736:Horses; D007279:Injections, Subcutaneous; D011994:Recombinant Proteins
chemical_list: D011994:Recombinant Proteins; D004921:Erythropoietin; D000068256:Darbepoetin alfa; D000068817:Epoetin Alfa