title: Characterization of immunoaffinity purified peptidoglycan-associated lipoprotein of Actinobacillus actinomycetemcomitans.
abstract: Peptidoglycan-associated lipoprotein (PAL) is a highly conserved structural outer membrane protein among Gram-negative bacteria. In some species, it is proinflammatory and released extracellularly. We purified a newly identified PAL (AaPAL) of a periodontal pathogen Actinobacillus actinomycetemcomitans by using AaPAL antipeptide antibodies coupled to immunoaffinity chromatography column. No protein impurities originating in A. actinomycetemcomitans were found in the final product. Sera from patients infected by A. actinomycetemcomitans recognized the purified AaPAL. The present purification method seems to be suitable for isolation of AaPAL and probably PALs of other bacterial species, and applicable in studies investigating proinflammatory mechanisms of A. actinomycetemcomitans.
authors.affiliation: Division of Oral Microbiology, Umeå University, Umeå SE-90185, Sweden.
authors.forename: Riikka
authors.initials: R
authors.lastname: Ihalin
authors.forename: Maribasappa
authors.initials: M
authors.lastname: Karched
authors.forename: Kjell
authors.initials: K
authors.lastname: Eneslätt
authors.forename: Sirkka
authors.initials: S
authors.lastname: Asikainen
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry* / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D015151:Immunoblotting; D008074:Lipoproteins / Q000302:isolation & purification*; D010457:Peptidoglycan / Q000302:isolation & purification*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins; D010457:Peptidoglycan