Rotaviral RNA found in wastewaters from hospital laundry.
The aim of this prospective study was to determine the presence of rotaviral RNA in water from a hospital laundry. Since rotaviruses are known as major causal agents of diarrhoea in humans, it is necessary that laundering hospital textiles results in efficient chemo-thermal disinfection, thus minimizing the possibility of transmission of rotaviruses to immune-compromised patients in hospitals. RT-PCR and second round PCR for gene amplification using specific primers, succeeding ultra-filtration and RNA isolation, was used to determine the presence of rotaviral RNA in water samples. The results show that rotaviral RNA was found in wastewater after the washing process, thus confirming an inadequate disinfecting effect of the examined laundering procedures.
Fijan
Sabina
S
Institute of Textiles, Faculty of Mechanical Engineering, University of Maribor, Smetanova ulica 17, SI-2000 Maribor, Slovenia. sabina.fijan@uni-mb.si
Poljsak-Prijatelj
Mateja
M
Steyer
Andrej
A
Koren
Srecko
S
Cencic
Avrelija
A
Sostar-Turk
Sonja
S
D001512:Bedding and Linens; D004203:Disinfection; D006801:Humans; D007847:Laundry Service, Hospital; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000635:transmission; D014866:Waste Products
D012367:RNA, Viral; D014866:Waste Products