Phagocytosis by Lymnaea stagnalis haemocytes: a potential role for phosphatidylinositol 3-kinase but not protein kinase A.
The molecular events that regulate phagocytosis, an important innate immune response, in invertebrate defence cells (haemocytes) are poorly understood. Lymnaea stagnalis haemocytes were used as a model to elucidate the role of cell signalling pathways in phagocytosis by molluscan defence cells. The phosphatidylinositol 3-kinase (PI3-K) inhibitor, LY294002, significantly impaired haemocyte phagocytic activity in a dose-responsive manner with 10 microM LY294002 reducing internalization of fluorescent-conjugated Escherichia coli by 62% (P < or = 0.001). In contrast, the protein kinase A (PKA) inhibitor KT5720 was without effect. Therefore, PI3-K, but not PKA, appears to control phagocytosis by haemocytes in these gastropod molluscs.
Plows
Louise D
LD
School of Life Sciences, Kingston University, Kingston-upon-Thames, Surrey KT1 2EE, UK.
Cook
Richard T
RT
Davies
Angela J
AJ
Walker
Anthony J
AJ
D000818:Animals; D002227:Carbazoles / Q000494:pharmacology; D002867:Chromones / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006434:Hemocytes / Q000187:drug effects / Q000201:enzymology*; D007211:Indoles / Q000494:pharmacology; D008195:Lymnaea / Q000502:physiology*; D009025:Morpholines / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases / Q000187:drug effects / Q000378:metabolism*; D011758:Pyrroles / Q000494:pharmacology
D002227:Carbazoles; D002867:Chromones; D004791:Enzyme Inhibitors; D007211:Indoles; D009025:Morpholines; D011758:Pyrroles; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; C057416:KT 5720; D019869:Phosphatidylinositol 3-Kinases; D017868:Cyclic AMP-Dependent Protein Kinases