[Proliferation and differentiation characteristics of human bone marrow mesenchymal stem cells during ex-vivo expansion].
OBJECTIVE
To investigate the effects of ex-vivo expansion on proliferative ability, pluripotentiality and other biologic characteristics of human bone marrow mesenchymal stem cells(MSCs).


METHODS
MSCs were isolated from human costal bone and passaged under the same culture conditions. At each passage, the characteristics of proliferation kinetics, osteogenic, chondrogenic, adipogenic differentiation potential were analyzed, and cell morphology, surface markers and cell cycle were investigated as well.


RESULTS
The proliferative ability and osteogenic, adipogenic differentiation potential decreased during culture expansion, while chondrogenic differentiation potential had no significant changes. MSCs maintained their multiple differentiation potential during their life-span. For each passage, the positive ratio of CD29, CD44, CD105 were all above 90% and the negative ratio of CD14, CD34, CD45 were below 4%.


CONCLUSIONS
Culture expansion causes MSCs to gradually lose their stem cell properties. During ex-vivo expansion of hMSCs, the osteogenic and adipogenic differentiation potential are more likely to lose than chondrogenic differentiation potential. Multiple differentiation potential is conserved longer than self-renewal capacity. MSCs before 7th passage can be a valuable subject for basic research and clinical application.
Hu
Jing-bo
JB
The State Key Laboratory of Bioreactor Engineering, East China University of Sciences and Technology, Shanghai 200237, China. jingbo_hu@yahoo.com.cn
Zhou
Yan
Y
Jiang
Dan-dan
DD
Tan
Wen-song
WS
D050156:Adipogenesis / Q000502:physiology; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D000071063:Endoglin; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism
D015703:Antigens, CD; D018952:Antigens, CD34; C064206:ENG protein, human; D000071063:Endoglin; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D018950:Lipopolysaccharide Receptors; D011956:Receptors, Cell Surface; D017493:Leukocyte Common Antigens