title: Expression of Pin1, a peptidyl-prolyl isomerase, in the ovaries of eCG/hCG-treated immature female mice.
abstract: Protein phosphorylation on certain serine or threonine residues preceding proline (Ser/Thr-Pro) is a pivotal signaling mechanism in diverse cellular processes. Pin1 is a highly conserved enzyme that isomerizes only the phosphorylated Ser/Thr-Pro bonds in certain proteins, thereby inducing conformational changes. Although much protein is phosphorylated in the ovary, the role of Pin1 in the ovary is still unknown. The purpose of this study is to investigate the effects of gonadotropins on protein and mRNA expression of Pin1 in mice ovaries. Quantitative PCR analysis showed that the expression of Pin1 mRNA significantly increased in the ovaries of equine chorionic gonadotropin (eCG)-treated mice compared with those of untreated mice (P<0.05). However, human chorionic gonadotropin (hCG) attenuated the expression of Pin1 mRNA increased by eCG. The protein level of Pin1 showed the same tendency as the expression of mRNA. The mRNA expression of E2F transcription factor, which controlled the expression of Pin1, was significantly decreased in the eCG-treated ovaries compared with the controls (P<0.05). These observations suggest that gonadotropins may regulate the expression of Pin1 without E2F transcription factor, indicating that Pin1 might be an important factor for protein signal transduction during follicular development.
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Japan. shimizut@obihiro.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Hirotada
authors.initials: H
authors.lastname: Akiyama
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Abe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sasada
authors.forename: Eimei
authors.initials: E
authors.lastname: Sato
authors.forename: Akio
authors.initials: A
authors.lastname: Miyamoto
authors.forename: Takafumi
authors.initials: T
authors.lastname: Uchida
mesh_terms: D000818:Animals; D015153:Blotting, Western; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D050684:E2F Transcription Factors / Q000378:metabolism; D005260:Female; D015292:Glycoprotein Hormones, alpha Subunit / Q000494:pharmacology*; D006062:Gonadotropins / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D015233:Models, Statistical; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D010053:Ovary / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012694:Serine / Q000737:chemistry; D015398:Signal Transduction; D013912:Threonine / Q000737:chemistry; D014158:Transcription, Genetic
chemical_list: D006063:Chorionic Gonadotropin; D050684:E2F Transcription Factors; D015292:Glycoprotein Hormones, alpha Subunit; D006062:Gonadotropins; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D012333:RNA, Messenger; D013912:Threonine; D012694:Serine; D012313:RNA; C000606846:PIN1 protein, human; D019696:Peptidylprolyl Isomerase; C000606850:Pin1 protein, mouse