Fourier transform infrared and raman spectroscopy for characterization of Listeria monocytogenes strains.
The purpose of this study was to characterize the variation in biochemical composition of 89 strains of Listeria monocytogenes with different susceptibilities towards sakacin P, using Fourier transform infrared (FTIR) spectroscopy and Raman spectroscopy. The strains were also analyzed using amplified fragment length polymorphism (AFLP) analysis. Based on their susceptibilities to sakacin P, the 89 strains have previously been divided into two groups. Using the FTIR spectra and AFLP data, the strains were basically differentiated into the same two groups. Analyses of the FTIR and Raman spectra revealed that the strains in the two groups contained differences in the compositions of carbohydrates and fatty acids. The relevance of the variation in the composition of carbohydrates with respect to the variation in the susceptibility towards sakacin P for the L. monocytogenes strains is discussed.
Oust
Astrid
A
Matforsk AS, Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway. astrid.oust@matforsk.no
Møretrø
Trond
T
Naterstad
Kristine
K
Sockalingum
Ganesh D
GD
Adt
Isabelle
I
Manfait
Michel
M
Kohler
Achim
A
D015373:Bacterial Typing Techniques; D001430:Bacteriocins / Q000494:pharmacology*; D002241:Carbohydrates / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008089:Listeria monocytogenes / Q000737:chemistry* / Q000145:classification* / Q000187:drug effects; D012150:Polymorphism, Restriction Fragment Length; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
D001430:Bacteriocins; D002241:Carbohydrates; D005227:Fatty Acids