title: [In vitro inhibitory effect of 23-HBA on angiogenesis].
abstract: OBJECTIVE
  To study the inhibitory effect of 23-HBA on angiogenesis in vitro.
  
  
  METHODS
  The effect of 23-hydroxy butulinic acid (23-HBA) on the in vitro proliferation of human microcapillary endothelial cells(HMECs) was examined by sulfonylrhodamine B (SRB) assay. The effect of 23-HBA on endothelial cell migration, and tubule formation on Matrigel was also observed. The CD31 expression in HMECs was dectected by immunohistochemical staining.
  
  
  RESULTS
  The proliferation of HMECs was inhibited significantly by 23-HBA with IC(50) being 40.44 mg/L. 23-HBA inhibited endothelial cell migration and tubule formation in a dose-dependent manner. The expression of CD31 in HMECs was reduced after treatment with 10 mg/L 23-HBA.
  
  
  CONCLUSIONS
  23-HBA can inhibit angiogenesis in vitro, which would become a promising antiangiogenic drug.
authors.affiliation: State Key Laboratory of Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China. jmj16888@yahoo.com.cn
authors.forename: Meng-jun
authors.initials: MJ
authors.lastname: Jiang
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Yao-yuan
authors.initials: YY
authors.lastname: Zhou
authors.forename: Rong-jun
authors.initials: RJ
authors.lastname: Zhang
authors.forename: Guo-xian
authors.initials: GX
authors.lastname: Cao
authors.forename: Gang-ming
authors.initials: GM
authors.lastname: Cai
authors.forename: Guang-ji
authors.initials: GJ
authors.lastname: Wang
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: C468651:23-hydroxybetulinic acid; D020533:Angiogenesis Inhibitors; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014315:Triterpenes