T-cell recognition of a prostate specific antigen is not sufficient to induce prostate tissue destruction.
METHODS
The ability of CD8(+) T-cells to induce prostate inflammation was examined using a prostate ovalbumin expressing transgenic mouse (POET) and/or adoptive transfer of T-cell receptor (TCR) transgenic T-cells (OT-I) that specifically recognize ovalbumin. Localization of inflammatory cells to prostate tissue was examined following T-cell activation via endogenous prostatic antigen, recombinant type 5 adenovirus carrying the gene coding ovalbumin (Ad5-mOVA), or adoptive transfer of in vitro antigen stimulated OT-I cells.


RESULTS
Ovalbumin specific OT-I cells were activated by autologous prostate antigen and trafficked to the prostate, but did not induce inflammation unless present in overwhelming numbers ( approximately 65% of CD8(+) T-cells). Activation of antigen specific CD8(+) T-cells in vitro (peptide pulsed antigen presenting cells) or in vivo (Ad5-mOVA) induced transitory prostate inflammation, without induction of prostate pathology, regardless of CD4(+) T-cell availability. Inflammation also was observed in OT-I x POET mice but again, pathological effects were not observed.


CONCLUSIONS
T lymphocytes specific for a prostate antigen are capable of inducing inflammatory infiltration of prostatic tissue rapidly following activation, but do not produce pathological prostate injury.
Lees
Jason R
JR
Interdisciplinary Program in Immunology, University of Iowa, Iowa City, Iowa 52242, USA.
Charbonneau
Bridget
B
Hayball
John D
JD
Diener
Kerrilyn
K
Brown
Michael
M
Matusik
Robert
R
Cohen
Michael B
MB
Ratliff
Timothy L
TL
D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D017931:DNA Primers; D007150:Immunohistochemistry; D007249:Inflammation; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000235:genetics*; D011467:Prostate / Q000276:immunology / Q000473:pathology*; D017430:Prostate-Specific Antigen / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013950:Thymus Gland / Q000276:immunology; D001743:Urinary Bladder / Q000276:immunology / Q000473:pathology
D016207:Cytokines; D017931:DNA Primers; D010047:Ovalbumin; D017430:Prostate-Specific Antigen