title: 9alpha,11beta-PGF2 and its stereoisomer PGF2alpha are novel agonists of the chemoattractant receptor, CRTH2.
abstract: CRTH2 is a recently described chemoattractant receptor for the prostaglandin, PGD(2), expressed by Th2 cells, eosinophils and basophils, and believed to play a role in allergic inflammation. Here we describe the potency of several PGD(2) metabolites at the receptor to induce cell migration and activation. We report for the first time that the PGD(2) metabolite, 9alpha,11beta-PGF(2), and its stereoisomer, PGF(2alpha), are CRTH2 agonists. 9alpha,11beta-PGF(2) is a major metabolite produced in vivo following allergen challenge, whilst PGF(2alpha) is generated independently of PGD synthetase, with implications for CRTH2 signalling in the presence or absence of PGD(2) production.
authors.affiliation: Leukocyte Biology Section, NHLI Division, Imperial College Faculty of Medicine, South Kensington, London SW7 2AZ, UK.
authors.forename: Hilary
authors.initials: H
authors.lastname: Sandig
authors.forename: David
authors.initials: D
authors.lastname: Andrew
authors.forename: Ashley A
authors.initials: AA
authors.lastname: Barnes
authors.forename: Ian
authors.initials: I
authors.lastname: Sabroe
authors.forename: James
authors.initials: J
authors.lastname: Pease
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D015237:Dinoprost / Q000737:chemistry / Q000378:metabolism*; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D010120:Oxytocics / Q000737:chemistry / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000819:agonists* / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000819:agonists* / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D010120:Oxytocics; D011971:Receptors, Immunologic; D011982:Receptors, Prostaglandin; D015237:Dinoprost; C036293:prostaglandin D2 receptor