Isoelectric point determination of proteins and other macromolecules: oscillating method.
A program written in Visual Basic has been developed to calculate the isoelectric point of proteins and other macromolecules bearing acid-basic residues. The pI value can be theoretically calculated with the precision required. The computer automatically supplies a representation of the charge of the protein versus pH values. The corresponding values can also be obtained, on command, in the form of table.
Sillero
Antonio
A
Facultad de Medicina, Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC/UAM, Madrid, Spain. antonio.sillero@uam.es
Maldonado
Andres
A
D003198:Computer Simulation; D007526:Isoelectric Point; D046911:Macromolecular Substances / Q000737:chemistry*; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry*; D012984:Software; D012985:Software Design
D046911:Macromolecular Substances; D011506:Proteins