D3 dopamine receptor mRNA is elevated in T cells of schizophrenic patients whereas D4 dopamine receptor mRNA is reduced in CD4+ -T cells.
The expression of dopamine receptors was examined in purified human neutrophils, monocytes, B cells, natural killer cells and CD4+ - and CD8+ -T lymphocytes by RT-PCR. In healthy subjects, D1 and D2 receptors were not expressed in leukocytes. Real Time PCR for dopamine receptors D3 and D4 disclosed that D3 receptors are expressed in T cells and natural killer cells and D4 receptors in CD4+ -T cells. The comparison of schizophrenic patients with sex- and age-matched controls revealed a significantly higher expression of D3 receptor mRNA in T cells of schizophrenic patients, whereas D4 receptor mRNA in CD4+ -T cells was downregulated.
Boneberg
Eva-Maria
EM
Biotechnology Institute Thurgau, Konstanzer Strasse 19, 8274 Taegerwilen, Switzerland. Eva.Maria.Boneberg@bitg.ch
von Seydlitz
Elisabeth
E
Pröpster
Karl
K
Watzl
Hans
H
Rockstroh
Brigitte
B
Illges
Harald
H
D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D050637:Receptors, Dopamine D3 / Q000276:immunology / Q000378:metabolism*; D050638:Receptors, Dopamine D4 / Q000276:immunology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012559:Schizophrenia / Q000276:immunology* / Q000378:metabolism
D012333:RNA, Messenger; D050637:Receptors, Dopamine D3; D050638:Receptors, Dopamine D4