title: [Effects of inhibiting SDF-1 expression by RNA interference on adhesion and drug sensitivity of Jurkat cells co-cultured with bone marrow stromal cells].
abstract: OBJECTIVE
  To observe the effects of inhibiting stromal cell derived factor-1 (SDF-1) expression by RNA interference (RNAi) on adhesion and drug sensitivity of Jurkat cells co-cultured with bone marrow stromal cells.
  
  
  METHODS
  SDF-1 specific short hairpin RNA (shRNA) expressing plasmid was transferred into cultured human acute leukemic bone marrow stromal cells, positive clones were isolated by screening G418 resistance (Group A) , SDF-1 protein level in culture supernatant was detected by enzyme-linked immunosorbent assay (ELISA). The adhesion rates to bone marrow stromal cells layer and the drug sensitivity to doxorubicin of co-cultured Jurkat cells were detected by cell counting and MTT assay, respectively. The un-transfected bone marrow stromal cells of acute leukemia patient (Group B) or normal subject (Group C) were taken as control.
  
  
  RESULTS
  The level of secreted SDF-1 protein (pg/10(5) cells/week) in the supernatants of Group A, B and C were 1920 +/- 205, 12,370 +/- 1355 and 6620 +/- 770, respectively. Of co-cultured Jurkat cells in Group A, B and C, the adhesion rates after 24 h co-culturing were (28.8 +/- 2.6)%, (57.4 +/- 3.8)% and (45.2 +/- 4.0)%, respectively, and the IC50 values of doxorubicin were 585, 6162 and 1758 nmol/L, respectively.
  
  
  CONCLUSIONS
  Down-regulating SDF-1 expression of bone marrow stromal cells by RNAi reduces adhesion rates and enhances drug sensitivity to doxorubicin of their co-cultured Jurkat cells.
authors.affiliation: Department of Hematology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Yang
authors.forename: Pei-Yan
authors.initials: PY
authors.lastname: Kong
authors.forename: Cheng
authors.initials: C
authors.lastname: Chang
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.forename: Dong-Feng
authors.initials: DF
authors.lastname: Zeng
authors.forename: Xian-Gui
authors.initials: XG
authors.lastname: Peng
authors.forename: Zhan-Zhong
authors.initials: ZZ
authors.lastname: Shi
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Wang
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism*; D002448:Cell Adhesion; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics* / Q000378:metabolism; D018920:Coculture Techniques; D019008:Drug Resistance, Neoplasm; D015870:Gene Expression; D006801:Humans; D019169:Jurkat Cells; D034622:RNA Interference; D017154:Stromal Cells / Q000378:metabolism*
chemical_list: D054377:Chemokine CXCL12