title: An outbreak of dengue fever in periurban slums of Chandigarh, India, with special reference to entomological and climatic factors.
abstract: BACKGROUND
  Dengue viral infection is one of the most important public health problem in tropical countries.
  
  
  OBJECTIVE
  An outbreak of dengue fever was investigated in a periurban slum area of Chandigarh, India, during September to December, 2002.
  
  
  METHODS
  Blood samples from 218 patients and 30 apparently healthy contacts were tested for dengue-specific immunoglobulin M (IgM) and IgG antibodies including 80 acute samples collected within 5 days of illness were subjected for virus isolation in newborn mice. The average temperature, rainfall, and humidity of the epidemic year were compared with the number of dengue cases.
  
  
  METHODS
  statistical significance was found out using c2-test.
  
  
  RESULTS
  A total of 76 cases were positive by either dengue IgM capture assay (n = 57) or virus isolation (n = 17) or both (n = 2). Fifteen of nineteen viral isolates subjected for typing by type-specific multiplex reverse transcription-polymerase chain reaction were found to be of dengue virus. High rainfall and humidity with the temperature range from 21 degrees C to 33 degrees C during the months of August and September might have favored the breeding of mosquitoes, thus leading to an increase in the number of dengue cases in October and November, 2002.
  
  
  CONCLUSIONS
  The present outbreak thus emphasizes the need for continuous sero epidemiological and entomological surveillance for the timely implementation of effective dengue control programme.
authors.affiliation: Department of Virology, Post Graduate Institute of Medical Education & Research, General Hospital, Sector-16, Chandigarh., India. rathopgi@yahoo.com
authors.forename: R K
authors.initials: RK
authors.lastname: Ratho
authors.forename: B
authors.initials: B
authors.lastname: Mishra
authors.forename: J
authors.initials: J
authors.lastname: Kaur
authors.forename: N
authors.initials: N
authors.lastname: Kakkar
authors.forename: K
authors.initials: K
authors.lastname: Sharma
mesh_terms: D000293:Adolescent; D000328:Adult; D000330:Aedes; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016009:Chi-Square Distribution; D002648:Child; D002980:Climate; D003715:Dengue / Q000453:epidemiology*; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D051379:Mice; D008875:Middle Aged; D011204:Poverty Areas; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012698:Serologic Tests; D014505:Urban Population
chemical_list: D000914:Antibodies, Viral