title: The quorum-sensing negative regulator RsaL of Pseudomonas aeruginosa binds to the lasI promoter.
abstract: A mutation in the rsaL gene of Pseudomonas aeruginosa produces dramatically higher amounts of N-acyl homoserine lactone with respect to the wild type, highlighting the key role of this negative regulator in controlling quorum sensing (QS) in this opportunistic pathogen. The DNA binding site of the RsaL protein on the rsaL-lasI bidirectional promoter partially overlaps the binding site of the LasR protein, consistent with the hypothesis that RsaL and LasR could be in binding competition on this promoter. This is the first direct demonstration that RsaL acts as a QS negative regulator by binding to the lasI promoter.
authors.affiliation: Department of Biology, University Roma Tre, Viale G. Marconi 446, 00146, Rome, Italy.
authors.forename: Giordano
authors.initials: G
authors.lastname: Rampioni
authors.forename: Iris
authors.initials: I
authors.lastname: Bertani
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Zennaro
authors.forename: Fabio
authors.initials: F
authors.lastname: Polticelli
authors.forename: Vittorio
authors.initials: V
authors.lastname: Venturi
authors.forename: Livia
authors.initials: L
authors.lastname: Leoni
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D021901:DNA, Intergenic / Q000235:genetics; D015536:Down-Regulation; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000502:physiology*; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D021901:DNA, Intergenic; C081237:LasI protein, Pseudomonas aeruginosa; D012097:Repressor Proteins; C118377:rsaL protein, Pseudomonas aeruginosa