T-kininogen induces endothelial cell proliferation.
Basal proliferation of endothelial cells increases with age, and this might play a role in the etiology of age-related vascular diseases, as well as angiogenesis. Serum kininogen levels increase during aging in rats and humans, and T-kininogen (T-KG) can affect proliferative homeostasis in several cell models. Both kinins and kininogens have been shown previously to be angiogenic through activation of endothelial cell proliferation, and here we show that exposure of endothelial cells to T-KG results in vigorous cell proliferation, accompanied by ERK/AKT activation. In our experiments, the proliferative response requires B1 and B2 kinin receptors, even though kinins are not released from the precursor. We hypothesize that the age-related increase in T-KG could play a significant role in the age-related dysregulation of vascular physiology and function.
Pérez
Viviana
V
Instituto de Ciencias Biomédicas, Programa de Biología Celular y Molecular and Centro FONDAP de Estudios Moleculares de la Célula, Facultad de Medicina, Universidad de Chile, Independencia 1027, Santiago, Chile.
Leiva-Salcedo
Elías
E
Acuña-Castillo
Claudio
C
Aravena
Mauricio
M
Gómez
Christian
C
Sabaj
Valeria
V
Colombo
Alicia
A
Nishimura
Sumiyo
S
Pérez
Claudio
C
Walter
Robin
R
Sierra
Felipe
F
D000375:Aging / Q000502:physiology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D007704:Kininogens / Q000378:metabolism / Q000502:physiology*; D051058:Oncogene Protein v-akt / Q000378:metabolism; D051381:Rats; D018000:Receptors, Peptide / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology
D007704:Kininogens; D018000:Receptors, Peptide; C044865:kininogen receptor; D051058:Oncogene Protein v-akt; D048049:Extracellular Signal-Regulated MAP Kinases