title: Periodic direct current does not promote wound closure in an in vitro dynamic model of cell migration.
abstract: OBJECTIVE
  A prevailing paradigm is that electrical fields can promote cell migration and tissue healing. To further validate this paradigm, we tested the hypothesis that periodic direct current (DC) can enhance wound closure using an in vitro dynamic model of cell migration.
  
  
  RESULTS
  Layers of primary fibroblasts were wounded and treated with DC under various voltages. Repair area, cell velocity, and directionality as well as lamellipodium area were evaluated at different times. Direct current had no beneficial effect on cell migration. Moreover, prolonged stimulation under the highest voltage led to significant reduction in wound closure and cell velocity. The reduction of membrane protusions in stimulated cells may be associated with the deleterious effect of DC.
  
  
  CONCLUSIONS
  Contrary to the authors' expectations, they found that periodic DC did not promote wound closure, a finding that emphasizes the need to clarify the complex effects of electrical fields on migrating cells.
authors.affiliation: Department of Rehabilitation, Laval University, Quebec City, Canada G1V 4G2.
authors.forename: Charles
authors.initials: C
authors.lastname: Godbout
authors.forename: Jérôme
authors.initials: J
authors.lastname: Frenette
mesh_terms: D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D004558:Electric Stimulation; D005347:Fibroblasts / Q000502:physiology* / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D008853:Microscopy; D008954:Models, Biological; D051381:Rats; D014741:Video Recording; D014945:Wound Healing / Q000502:physiology*