title: Detection of electrophysiological indicators of neurotoxicity in human and rat brain slices by a three-dimensional microelectrode array.
abstract: Electrophysiological techniques for the assessment of in vitro neurotoxicology have several advantages over other currently-used methods (for example, morphological techniques), including the ability to detect damage at a very early stage. Novel recording techniques based on microelectrode arrays are available, and could improve recording power. In this study, we investigated how a three-dimensional microelectrode array detects the electrophysiological endpoints of neurotoxicity. We conclude that electrophysiology sensitively reveals neurotoxic actions, and that three-dimensional microelectrode arrays could be proposed for use in neurotoxicology as recording tools that allow easy and sensitive multisite recording, from both rodent and human brain tissue.
authors.affiliation: Institute for Physiology, Westfalische Wilhelms-University, Münster, Germany.
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Köhling
authors.forename: Raffaella
authors.initials: R
authors.lastname: Melani
authors.forename: Uwe
authors.initials: U
authors.lastname: Koch
authors.forename: Erwin-Josef
authors.initials: EJ
authors.lastname: Speckmann
authors.forename: Milena
authors.initials: M
authors.lastname: Koudelka-Hep
authors.forename: Pierre
authors.initials: P
authors.lastname: Thiébaud
authors.forename: Maurizio
authors.initials: M
authors.lastname: Balestrino
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000503:physiopathology*; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D005071:Evoked Potentials / Q000187:drug effects; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity*; D006801:Humans; D007608:Kainic Acid / Q000633:toxicity*; D008297:Male; D008839:Microelectrodes; D019579:Neocortex / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D014298:Trimethyltin Compounds / Q000633:toxicity*
chemical_list: D018690:Excitatory Amino Acid Agonists; D014298:Trimethyltin Compounds; C046488:trimethyltin; D007608:Kainic Acid