Isolation and purification of clemastanin B and indigoticoside A from Radix Isatidis by high-speed counter-current chromatography.
A preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of ethyl acetate-n-butanol-water (2:7:9, v/v/v) was successfully performed to isolate and separate clemastanin B and indigoticoside A from the plant of Radix Isatidis, a traditional Chinese medicine. A total of 59.2 mg clemastanin B and 66.1 mg indigoticoside A with purities of 94.6% and 99.0% determined by high performance liquid chromatography (HPLC) were obtained in one-step elution from 250 mg crude extract, which contained clemastanin B 24.8% and indigoticoside A 28.4%, and the recoveries of clemastanin B and indigoticoside A were 90.3% and 92.2%, respectively. The chemical structure was identified by IR, MS, 1H NMR and 13C NMR.
Peng
Jinyong
J
Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China.
Fan
Guorong
G
Wu
Yutian
Y
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D005663:Furans; D010936:Plant Extracts; D012997:Solvents; C507644:clemastanin B; C507645:indigoticoside A