title: The endophytic fungus Trametes hirsuta as a novel alternative source of podophyllotoxin and related aryl tetralin lignans.
abstract: The aryl tetralin lignans are synthesized by Podophyllum sps. and are in great demand worldwide due to their use in synthesis of topoisomerase inhibitors. However, the sustained production of these aryl tetralin lignans requires large-scale harvesting from the natural environments, which has resulted in the plant-endangered status. In view of the difficulties in their total chemical synthesis, cultivation and failure of metabolic engineering approaches, there is a need to search for alternative sources of production of aryl tetralin lignans. We unequivocally established the methodology for isolation, identification, and characterization of a novel fungal endophyte (Trametes hirsuta) that produces aryl tetralin lignans consistently as shown by HPLC, LC-MS, LC/MS-MS and (1)H NMR. The lignans produced by the microorganism are biologically active, and exhibit potent antioxidant, anticancer and radioprotective properties. This strategy promises to improve the production of these therapeutically important compounds at lower costs.
authors.affiliation: Natural Products Chemistry Division, Regional Research Laboratory (CSIR), Jammu 180001, India. rks@inmas.org
authors.forename: Satish Chandra
authors.initials: SC
authors.lastname: Puri
authors.forename: Asiya
authors.initials: A
authors.lastname: Nazir
authors.forename: Raman
authors.initials: R
authors.lastname: Chawla
authors.forename: Rajesh
authors.initials: R
authors.lastname: Arora
authors.forename: S
authors.initials: S
authors.lastname: Riyaz-Ul-Hasan
authors.forename: Touseef
authors.initials: T
authors.lastname: Amna
authors.forename: Bilal
authors.initials: B
authors.lastname: Ahmed
authors.forename: Vijeshwar
authors.initials: V
authors.lastname: Verma
authors.forename: Shikha
authors.initials: S
authors.lastname: Singh
authors.forename: Ravinder
authors.initials: R
authors.lastname: Sagar
authors.forename: Ashok
authors.initials: A
authors.lastname: Sharma
authors.forename: Raj
authors.initials: R
authors.lastname: Kumar
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Sharma
authors.forename: Ghulam Nabi
authors.initials: GN
authors.lastname: Qazi
mesh_terms: D002458:Cell Fractionation; D002851:Chromatography, High Pressure Liquid; D005658:Fungi / Q000166:cytology / Q000302:isolation & purification*; D007700:Kinetics; D017705:Lignans / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000528:radiation effects; D008954:Models, Biological; D059828:Plant Cells; D018514:Plant Structures / Q000166:cytology / Q000378:metabolism; D010944:Plants / Q000378:metabolism; D011034:Podophyllotoxin / Q000737:chemistry* / Q000378:metabolism; D011035:Podophyllum / Q000166:cytology / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013764:Tetrahydronaphthalenes / Q000737:chemistry*
chemical_list: D017705:Lignans; D013764:Tetrahydronaphthalenes; C095210:tetralin; D011034:Podophyllotoxin