The anti-obesity agent Orlistat is associated to increase in colonic preneoplastic markers in rats treated with a chemical carcinogen.
Orlistat is an anti-obesity agent that increases the fecal fat excretion, which promotes colon carcinogenesis. Therefore, the present study was designed to verify the effects of Orlistat on the formation of rat colonic aberrant crypt foci (ACF) and cell proliferation evaluated by the PCNA method. Male Wistar rats received either a standard diet or a high fat diet (HFD), supplemented or not with Orlistat (200mg/kg chow) and two doses of the carcinogen dimethyl-hydrazine (25mg/Kg). After 30 days, Orlistat was associated to a significant increase in the number of colonic ACFs and cell proliferation in DMH-treated animals, independently of the HFD.
Garcia
Sérgio Britto
SB
Department of Pathology, Ribeirão Preto Medical School, University of São Paulo, USP, Avenida Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brazil. sbgarcia@fmrp.usp.br
Barros
Luane Taísa da Costa
LT
Turatti
Aline
A
Martinello
Flávia
F
Modiano
Patrícia
P
Ribeiro-Silva
Alfredo
A
Vespúcio
Marcelo Vinícius de Oliveira
MV
Uyemura
Sérgio Akira
SA
D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D000818:Animals; D019440:Anti-Obesity Agents / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects; D007783:Lactones / Q000633:toxicity*; D008049:Lipase / Q000037:antagonists & inhibitors; D008297:Male; D000077403:Orlistat; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D019440:Anti-Obesity Agents; D002273:Carcinogens; D007783:Lactones; D018809:Proliferating Cell Nuclear Antigen; D000077403:Orlistat; D008049:Lipase; D019813:1,2-Dimethylhydrazine