Homogeneity of the 16S rDNA sequence among geographically disparate isolates of Taylorella equigenitalis.
BACKGROUND
At present, six accessible sequences of 16S rDNA from Taylorella equigenitalis (T. equigenitalis) are available, whose sequence differences occur at a few nucleotide positions. Thus it is important to determine these sequences from additional strains in other countries, if possible, in order to clarify any anomalies regarding 16S rDNA sequence heterogeneity. Here, we clone and sequence the approximate full-length 16S rDNA from additional strains of T. equigenitalis isolated in Japan, Australia and France and compare these sequences to the existing published sequences.


RESULTS
Clarification of any anomalies regarding 16S rDNA sequence heterogeneity of T. equigenitalis was carried out. When cloning, sequencing and comparison of the approximate full-length 16S rDNA from 17 strains of T. equigenitalis isolated in Japan, Australia and France, nucleotide sequence differences were demonstrated at the six loci in the 1,469 nucleotide sequence. Moreover, 12 polymorphic sites occurred among 23 sequences of the 16S rDNA, including the six reference sequences.


CONCLUSIONS
High sequence similarity (99.5% or more) was observed throughout, except from nucleotide positions 138 to 501 where substitutions and deletions were noted.
Matsuda
M
M
Department of Bacteriology, Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, UK. matsuda@azabu-u.ac.jp
Tazumi
A
A
Kagawa
S
S
Sekizuka
T
T
Murayama
O
O
Moore
J E
JE
Millar
B C
BC
D000818:Animals; D001315:Australia / Q000453:epidemiology; D005602:France / Q000453:epidemiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D006734:Horse Diseases / Q000453:epidemiology / Q000382:microbiology*; D006736:Horses; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D020625:Taylorella equigenitalis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D012336:RNA, Ribosomal, 16S