In vitro re-mineralization of demineralized bone matrix in human serum.
The aim of this study was to develop an in vitro re-mineralization model in human serum. For this purpose a commercially produced demineralized human bone matrix (DBM) was incubated in samples of human serum pools obtained from two physiologically different groups. The first group consisted of young males and the second of older females. After incubation periods of 4 and 7 days at 37 degrees C, changes in the levels of calcium and inorganic phosphate content of the serum and DBM samples were measured. The results of the study showed that the change in mineral content of serum and DBM samples in both study groups was statistically significant. The decrease in serum calcium content and increase in DBM inorganic phosphate content were significant in the young group for longer incubation times. In the older group, both serum calcium and inorganic phosphate decreased and DBM mineral content increased for the same incubation time. When the two physiological groups were compared, statistically significant differences were identified for changes in mineral levels in both serum and the DBM samples. These data indicate that the mineral content of human serum decreases and that of DBM increases when these two materials are incubated together. These changes provide evidence for the re-mineralization of DBM. The model described here could also detect a difference in re-mineralization capability between two different groups of human sera.
Eskandari
Metin Manouchehr
MM
Department of Orthopedics and Traumatology, University of Mersin, School of Medicine, Turkey.
Gulcin Eskandari
Hatice
H
Aktas
Melih
M
Atik
Ugur
U
D000328:Adult; D001857:Bone Matrix / Q000502:physiology*; D002113:Calcification, Physiologic / Q000502:physiology*; D002118:Calcium / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D010758:Phosphorus / Q000097:blood; D044967:Serum / Q000737:chemistry*
D010758:Phosphorus; D002118:Calcium