Transient versus prolonged hyperlocomotion following lateral fluid percussion injury in mongolian gerbils.
Posttraumatic hyperactivity is a neurobehavioral symptom commonly seen in patients after traumatic brain injury (TBI). No useful animal model has yet been established for evaluation of this important symptom. We induced either mild (MILD, 0.7-0.9 atm) or moderate (MOD, 1.3-1.6 atm) lateral fluid percussion injury (LFPI) in Mongolian gerbils. Open-field and T-maze tests were used during a 7-day period after the trauma. All animals were perfusion fixed for histopathological examinations. Transient locomotor hyperactivity was found with a peak at 6 hr after injury in the MILD animals, whereas MOD animals showed prolonged and severe hyperlocomotion throughout the 7-day posttrauma period (P < 0.0001). Interestingly, the temporal profile of the posttraumatic hyperactivity was similar to that of the working memory deficit in both injury groups. Histological examination revealed significant neural tissue damages, including cortical necrosis, white matter rarefaction, and neuronal loss in the hippocampus in the ipsilateral hemisphere of the MOD animals, vs. only negligible changes in the MILD animals. Correlation analysis revealed that the volume of white matter lesions was significantly correlated with both posttraumatic hyperactivity (r = 0.591, P < 0.01) and working memory deficit (r = -0.859, P < 0.0001). Taken together, our findings confirm the successful reproduction of posttraumatic hyperactivity following experimental TBI. The posttraumatic hyperlocomotion probably shared pathomechanisms common to those of cognitive dysfunction caused by LFPI, supporting the speculation from previous studies that some neurobehavioral abnormities intimately correlate with TBI-induced cognitive dysfunction. Histopathologically, significant involvement of white matter damage in the posttraumatic functional deficits was indicated.
Li
Shihong
S
Department of Neurosurgery, Graduate School of Medicine, Tokyo Medical andDental University, Tokyo, Japan.
Kuroiwa
Toshihiko
T
Katsumata
Noriko
N
Ishibashi
Satoru
S
Sun
Li Yuan
LY
Endo
Shu
S
Ohno
Kikuo
K
D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal; D001794:Blood Pressure / Q000502:physiology; D001930:Brain Injuries / Q000150:complications* / Q000473:pathology; D002555:Cerebrospinal Fluid / Q000502:physiology; D004195:Disease Models, Animal; D005106:Exploratory Behavior / Q000502:physiology; D007839:Functional Laterality; D005849:Gerbillinae; D020547:Lateral Ventricles / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D018782:Maze Learning / Q000502:physiology; D009069:Movement Disorders / Q000209:etiology* / Q000473:pathology; D010474:Percussion / Q000379:methods*; D010808:Physical Examination / Q000379:methods; D013223:Statistics as Topic; D013997:Time Factors