Production of cloned pigs by nuclear transfer of preadipocytes established from adult mature adipocytes.
The aim of the present study was to determine whether porcine preadipocytes can be efficient donor cells for somatic cell nuclear transfer (SCNT) in pigs. Primary culture of porcine preadipocytes was established by de-differentiating mature fat cells taken from an adult pig. The cell cycle of the preadipocytes could be synchronized by serum starvation for 1 day, with a higher efficiency than control fetal fibroblasts. Incidence of premature chromosome condensation following nuclear transfer (NT) of preadipocytes was as high as that observed after NT with fetal fibroblasts. In vitro developmental rate of the NT embryos reconstructed with preadipocyte was equivalent to that of the fetal fibroblast derived embryos. Transfer of 732 NT embryos with preadipocytes to five recipients gave rise to five cloned piglets. These data demonstrate that preadipocyites collected from an adult pig are promising nuclear donor cells for pig cloning.
Tomii
Ryo
R
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University, Kawasaki, Kanagawa, Japan.
Kurome
Mayuko
M
Ochiai
Takashi
T
Wako
Naohiro
N
Ueda
Hideto
H
Hirakawa
Kazumasa
K
Kano
Koichiro
K
Nagashima
Hiroshi
H
D017667:Adipocytes / Q000166:cytology / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation; D019976:Cloning, Organism / Q000379:methods; D004624:Embryo Transfer; D005260:Female; D005333:Fetus / Q000166:cytology / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D013552:Swine / Q000502:physiology