title: Effect of nitric oxide and peroxynitrite on mucociliary transport function of experimental otitis media.
abstract: OBJECTIVE
  To determine the role of nitric oxide (NO) and peroxynitrite on mucociliary activity in experimental otitis media with effusion (OME).
  
  
  METHODS
  Twenty guinea pigs were divided into 1 control and 3 experimental groups; lipopolysaccharide (LPS), N(G)-nitro-L-arginine methyl ester (L-NAME), and uric acid (UA) groups. Ten ears were used in each group. OME was induced by transtympanic injection of LPS in experimental groups. Twenty-four hours after the transtympanic injection, dye transfer time (DTT) was measured and the temporal bone was taken for histopathologic examination. Expression of peroxynitrite was determined by immunohistochemical stain for 3-nitrotyrosine (3-NT).
  
  
  RESULTS
  Dye transfer time was significantly delayed in LPS group compared to control group; by contrast it was significantly reduced in L-NAME or UA treated groups (P < 0.01). Histopathologic examination showed reduced inflammation and mucosal thickening in the treatment groups when compared to LPS group. These findings, however, were not statistically significant. Immunoreactivity to 3-NT was intense in LPS group and decreased in the treatment groups (P < 0.05).
  
  
  CONCLUSIONS
  It is suggested that LPS induced mucociliary dysfunction in the middle ear by NO and peroxynitrite-mediated pathways.
authors.affiliation: Department of Otolaryngology-HNS, College of Medicine, The Catholic University of Korea, Our Lady of Mercy Hospital, 665 Bupyung-dong, Bupyung-gu, Incheon 403-720, Korea.
authors.forename: Eun-ju
authors.initials: EJ
authors.lastname: Jeon
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Park
authors.forename: Seung Kyun
authors.initials: SK
authors.lastname: Lee
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Yeo
authors.forename: Shi Nae
authors.initials: SN
authors.lastname: Park
authors.forename: Ki-Hong
authors.initials: KH
authors.lastname: Chang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000494:pharmacology; D006168:Guinea Pigs; D008070:Lipopolysaccharides / Q000494:pharmacology; D009079:Mucociliary Clearance / Q000187:drug effects / Q000502:physiology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010034:Otitis Media with Effusion / Q000503:physiopathology*; D030421:Peroxynitrous Acid / Q000502:physiology*; D011550:Pseudomonas aeruginosa; D014527:Uric Acid / Q000494:pharmacology
chemical_list: D016166:Free Radical Scavengers; D008070:Lipopolysaccharides; D030421:Peroxynitrous Acid; D014527:Uric Acid; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester