Assessment of a bioactive compound for its potential antiinflammatory property by tight junction permeability.
Lactobacillus probiotic strains are proving to be abundant sources of bioactive components, including antiinflammatory components. Lifree was made of fruits fermented by Lactobacillus paracasei, Lactobacillus reuterrii and Saccharomyces cerevisiae. This study was designed to test these compounds in cell assays measuring epithelial barrier function and proliferation in the first instance. Cell proliferation was measured in mouse fibroblasts cells (3T3NIH) and rat intestinal epithelial cells (IEC-6), and tight junction activity in the kidney epithelial cell line (MDCK). Tight junction permeability was assessed by measuring transepithelial electrical resistance (TER) across confluent monolayers, following the addition of Lifree with or without a challenge with EGTA. Lifree promoted tight junction formation and recovery following loss of TER from challenge with EGTA. On the other hand, Lifree did not stimulate cell growth in either 3T3NIH and IEC-6 cells. Lifree stimulates tight junction maintenance and formation, suggesting it may have potential antiinflammatory properties.
Bai
Young Hoon
YH
Research Division of Biological Science, Chosun University Medical School, Gwangju, 501-759, Korea.
Pak
Sok Cheon
SC
Lee
Seung Hoo
SH
Bae
Chun Sik
CS
Prosser
Colin
C
Stelwagen
Kerst
K
Lee
Jin Hee
JH
Park
Soon Duck
SD
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D005285:Fermentation; D005638:Fruit; D007778:Lactobacillus; D051379:Mice; D041681:NIH 3T3 Cells; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D019108:Tight Junctions / Q000187:drug effects*
D000893:Anti-Inflammatory Agents; D001688:Biological Products; D010936:Plant Extracts