Analysis of fatty aldehyde composition, including 12-methyltridecanal, in plasmalogens from longissimus muscle of concentrate- and pasture-fed bulls.
In a large study, 64 German Holstein and German Simmental bulls were randomly allocated to either an indoor concentrate system or periods of pasture feeding followed by a finishing period on a concentrate containing linseed to enhance the contents of beneficial fatty acids in beef. This paper reports the diet effects on the concentration of 12-methyltridecanal (12-MT) and further fatty aldehydes released from plasmalogens in the phospholipids of longissimus muscle of the bulls. Because of the trace level of the important odorant 12-MT in beef, the determination of fatty aldehydes in phospholipids was done by acidic hydrolysis and the reaction of the aldehydes with 2,4-DNPH followed by high-pressure liquid chromatography (HPLC) analysis. The diet affected the 12-MT concentrations in the muscle phospholipids of both breeds. Pasture feeding significantly increased the 12-MT concentrations up to 350 microg/100 g fresh muscle in the muscle phospholipids of German Holstein and German Simmental bulls as compared with the concentrate-fed bulls. Furthermore, pasture feeding resulted in a significant increase of n-octadecanal in the muscle phospholipids of both breeds up to 39.5 mg/100 g fresh muscle. The concentration of n-hexadecanal was not affected by the diet. Pasture feeding as compared to concentrate feeding significantly decreased the concentration of n-octadec-9-enal in the muscle phospholipids. Summarizing, pasture feeding increased the 12-MT concentration, which can be associated with meat of more intensive aroma and better taste.
Dannenberger
Dirk
D
Department of Muscle Biology and Growth, Research Institute for Biology of Farm Animals, Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, Germany.
Lorenz
Stephanie
S
Nuernberg
Gerd
G
Scollan
Nigel
N
Ender
Klaus
K
Nuernberg
Karin
K
D000447:Aldehydes / Q000032:analysis*; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004032:Diet; D005227:Fatty Acids / Q000032:analysis*; D008297:Male; D008460:Meat / Q000032:analysis; D018482:Muscle, Skeletal / Q000737:chemistry*; D009812:Odorants / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010955:Plasmalogens / Q000737:chemistry*
D000447:Aldehydes; D005227:Fatty Acids; D010743:Phospholipids; D010955:Plasmalogens; C001634:fatty aldehyde; C095952:12-methyltridecanal