title: [Study on the extraction and purification for total flavonoids in Lonicera japonica Thunb. and Chrysanthemum morifolium Tzvel].
abstract: OBJECTIVE
  To optimize the extraction and purification conditions for total flavonoids in Lonicera japonica Thunb. and Chrysanthemum morifolium Tzvel.
  
  
  METHODS
  Comparision of several extraction processes, the L9 (3(4)) orthogonal design and macroreticular absorption resin D101 purification technique were performed.
  
  
  RESULTS
  The optimum extraction conditions were 8 volume of 55% ethanol, 1 h refluence at 100 degrees C , water bath for 2 times. The extracted fluid was absorbed with the resin D101. The elutions were water, 30% enthanol, 70% enthanol. The obtained elution of 70% enthanol was evaporated and the content of total flavonoids was 62. 7%.
  
  
  CONCLUSIONS
  The method is simple, approved and fit for industrial production.
authors.affiliation: Jinling Pharmaceutical Co. Ltd, Nanjing, Jiangsu.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Tian
authors.forename: Xingwang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D031202:Chrysanthemum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D035264:Flowers / Q000737:chemistry; D031239:Lonicera / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D014867:Water
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D014867:Water; D000431:Ethanol