[RDP1258 on proliferation of rat splenocytes in vivo].
OBJECTIVE
To explore the immunosuppressive effects of a novel HLA-derived peptide, RDP1258, on rat splenocyte proliferation in vivo and its underlying mechanisms.


METHODS
RDP1258 was synthesized through artificial solid-phase synthesis method. Its effects on proliferative reaction of rat splenocytes in vivo were observed by (3)H-TDR incorporation method. And its effects on the heme oxygenase(HO) enzyme activity were analyzed by enzyme chemistry method.


RESULTS
The results showed that the synthetic HLA-derived peptide RDP1258 obviously inhibited the proliferation of rat splenocytes stimulated by isoantigen and mitogen. In vivo administration of RDP1258 peptide to rats resulted in upregulation of splenic HO activity and rapid upregulation of HO-1 protein expression.


CONCLUSIONS
HO-1 might participate in RDP1258 inhibiting the proliferation of rat splenocytes induced by mitogen and isoantigen in vivo.
Yi
Shan-hong
SH
Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. yshh881@sohu.com
Ye
Gang
G
Feng
Quan
Q
Wang
Ze-hou
ZH
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism; D007150:Immunohistochemistry; D007519:Isoantigens / Q000494:pharmacology; D008934:Mitogens / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000166:cytology* / Q000378:metabolism
D007519:Isoantigens; D008934:Mitogens; D009842:Oligopeptides; C122045:RDP 1258; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1