Proliferative senescence in hematopoietic stem cells during ex-vivo expansion.
The good outcome of hematopoietic stem cell (HSC) transplantation is hampered by low doses of CD34+ cell infusion. Transplanted HSCs undergo a replicative stress that causes accelerated senescence due to rapid telomere shortening. The expansion of human cord blood HSCs is instrumental in obtaining a large number of "good quality" cells, in terms of telomere length and telomerase activity compared to adult HSCs.
Piacibello
Wanda
W
Laboratory of Medical Oncology, Department of Oncological Sciences, IRCC Institute for Cancer Research and Treatment, University of Turin Medical School, Candiolo, Turin, Italy. wanda.piacibello@ircc.it
Gammaitoni
Loretta
L
Pignochino
Ymera
Y
D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology; D049109:Cell Proliferation; D016922:Cellular Senescence / Q000235:genetics / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D019098:Telomerase / Q000502:physiology; D016615:Telomere / Q000502:physiology
D018952:Antigens, CD34; D019098:Telomerase