Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering.
The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFlFFF-MALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (d(H)) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum d(H) was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.
Luo
Jian
J
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, PR China.
Leeman
Mats
M
Ballagi
Andras
A
Elfwing
Anders
A
Su
Zhiguo
Z
Janson
Jan-Christer
JC
Wahlund
Karl-Gustav
KG
D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D031521:Fractionation, Field Flow / Q000295:instrumentation / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D002479:Inclusion Bodies / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008027:Light; D008970:Molecular Weight; D012542:Scattering, Radiation; D013997:Time Factors
D049452:Green Fluorescent Proteins; D007544:Isopropyl Thiogalactoside