title: 6H,13H-Pyrazino[1,2-a;4,5-a']diindole analogs: probing the pharmacophore for allosteric ligands of muscarinic M2 receptors.
abstract: A series of 6H,13H-pyrazino[1,2-a;4,5-a']diindole analogs was synthesized in order to probe the pharmacophore hypothesis for allosteric ligands of muscarinic M(2) receptors. The 3D structure of the novel ring system was determined by means of NMR spectroscopy and X-ray diffraction revealing a totally flat geometry. Low binding affinities for the [(3)H]N-methylscopolamine-occupied M(2) receptors (reflected by EC(50,diss)) indicated that the spatial arrangement of the pharmacophore elements (two aromatic rings flanked by two cationic centers) incorporated in the bisquaternary analogs 5 and 6 is unfavorable for strong ligand-receptor interactions. Due to the structural similarity of the novel compounds to neuromuscular-blocking agents, their affinities (reflected by K(i)) to the muscle type of nicotinic acetylcholine receptors were also determined. The dimethyl and diallyl analogs 5 and 6 exhibited rather high affinities to the muscle type of nicotinic acetylcholine receptors, suggesting a pronounced neuromuscular-blocking activity. Compound 5 showed a 34-fold higher affinity for the muscle type nAChR than for the allosteric site of M(2) receptors.
authors.affiliation: Pharmaceutical Institute, University of Würzburg, Am Hubland, 97074 Würzburg, Germany. zlotos@pzlc.uni-wuerzburg.de
authors.forename: Darius P
authors.initials: DP
authors.lastname: Zlotos
authors.forename: Christian
authors.initials: C
authors.lastname: Tränkle
authors.forename: Aliaa
authors.initials: A
authors.lastname: Abdelrahman
authors.forename: Daniela
authors.initials: D
authors.lastname: Gündisch
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Radacki
authors.forename: Holger
authors.initials: H
authors.lastname: Braunschweig
authors.forename: Klaus
authors.initials: K
authors.lastname: Mohr
mesh_terms: D000494:Allosteric Regulation; D000495:Allosteric Site / Q000187:drug effects*; D000818:Animals; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D009132:Muscles; D009466:Neuromuscular Blocking Agents; D011719:Pyrazines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011869:Radioligand Assay; D043585:Receptor, Muscarinic M2 / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014101:Torpedo; D014961:X-Ray Diffraction
chemical_list: D007211:Indoles; D008024:Ligands; D009466:Neuromuscular Blocking Agents; D011719:Pyrazines; D043585:Receptor, Muscarinic M2