Detection of Epstein-Barr virus infection and gene expression in human tumors by microarray analysis.
Epstein-Barr virus (EBV) genome-chips are employed to determine the EBV infection rate and to reveal the gene expression patterns of EBV in tumor biopsies. These chips are produced with 71 consecutive PCR-amplified EBV DNA fragments of 1-3 kbp covering the entire EBV genome. The specificity of the EBV-chips is determined by hybridizing the DNA on the chips with biotin-labeled cDNA probes reverse transcribed from the mRNA of P3HR1 cells, which were B-cell infected latently by EBV. Hybridization results revealed only the expression of EBNA1, EBNA2, EBER1 and EBER2 in these cells. On the other hand, EBV lytic genes are expressed after the cells are treated with 12-O-tetradecanoylphorbol-13-acetate and sodium butyrate to induce the EBV lytic cycle. Fourty-four tumor biopsies from different organs are assayed with these chips, which showed many defined and interesting EBV gene expression patterns. This study demonstrates that the EBV-chip is useful for screening infection with EBV in tumors, which may lead to insights into tumorigenesis associated with this virus.
Li
Ching
C
Department of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan, ROC.
Chen
Ruey-Shyang
RS
Hung
Shang-Kai
SK
Lee
Yuan-Ti
YT
Yen
Ching-Yu
CY
Lai
Yi-Wen
YW
Teng
Ru-Hsiu
RH
Huang
Jia-Yan
JY
Tang
Yung-Cheng
YC
Tung
Chao-Ping
CP
Wei
Ta-Tung
TT
Shieh
Biehuoy
B
Liu
Shih-Tung
ST
D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000821:virology; D002051:Burkitt Lymphoma / Q000473:pathology; D002087:Butyrates / Q000494:pharmacology; D045744:Cell Line, Tumor; D004279:DNA, Viral / Q000235:genetics; D020031:Epstein-Barr Virus Infections / Q000821:virology*; D005069:Evaluation Studies as Topic; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D009369:Neoplasms / Q000821:virology*; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002087:Butyrates; D004279:DNA, Viral; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate