title: The DD-carboxypeptidase activity encoded by pbp4B is not essential for the cell growth of Escherichia coli.
abstract: The gene (pbp4B) encoding a putative DD-carboxypeptidase has been deleted in Escherichia coli and it is shown to be not essential for cell division. Disruption of the gene in a genetic background where all putative activities of DD-carboxypeptidases and/or DD-endopeptidases had been eliminated indicates that these activities are not required for cell growth in enterobacteria. The penicillin-binding capacity and a low DD-carboxypeptidase activity of PBP4B are demonstrated.
authors.affiliation: Centro de Biología Molecular Severo Ochoa CSIC-UAM, Campus de Cantoblanco, 28049, Madrid, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Vega
authors.forename: Juan A
authors.initials: JA
authors.lastname: Ayala
mesh_terms: D004150:Dipeptidases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D017353:Gene Deletion; D046915:Penicillin-Binding Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism
chemical_list: D046915:Penicillin-Binding Proteins; D004150:Dipeptidases; C019989:DD-carboxypeptidase-endopeptidase