Multiple-locus variable-number tandem repeat analysis for typing of Staphylococcus epidermidis.
We applied a high-resolution PCR-based typing method, multiple-locus variable-number tandem repeat analysis (MLVA), for discrimination of 30 multidrug-resistant clinical isolates of Staphylococcus epidermidis. The results of MLVA were congruent with results obtained by pulsed-field gel electrophoresis (PFGE). MLVA generated discrete character data, and its discriminatory capacity was comparable to that of PFGE.
Johansson
Anders
A
Department of Clinical Microbiology, Infectious Diseases, Umeå University, Sweden. anders.johansson@infdis.umu.se
Koskiniemi
Satu
S
Gottfridsson
Per
P
Wiström
Johan
J
Monsen
Tor
T
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D016680:Genome, Bacterial; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial