title: Caenorhabditis elegans survives atmospheric breakup of STS-107, space shuttle Columbia.
abstract: The nematode Caenorhabditis elegans, a popular organism for biological studies, is being developed as a model system for space biology. The chemically defined liquid medium, C. elegans Maintenance Medium (CeMM), allows axenic cultivation and automation of experiments that are critical for spaceflight research. To validate CeMM for use during spaceflight, we grew animals using CeMM and standard laboratory conditions onboard STS-107, space shuttle Columbia. Tragically, the Columbia was destroyed while reentering the Earth's atmosphere. During the massive recovery effort, hardware that contained our experiment was found. Live animals were observed in four of the five recovered canisters, which had survived on both types of media. These data demonstrate that CeMM is capable of supporting C. elegans during spaceflight. They also demonstrate that animals can survive a relatively unprotected reentry into the Earth's atmosphere, which has implications with regard to the packaging of living material during space flight, planetary protection, and the interplanetary transfer of life.
authors.affiliation: NASA Ames Research Center, Moffett Field, CA 94035-1000, USA.
authors.forename: Nathaniel J
authors.initials: NJ
authors.lastname: Szewczyk
authors.forename: Rocco L
authors.initials: RL
authors.lastname: Mancinelli
authors.forename: William
authors.initials: W
authors.lastname: McLamb
authors.forename: David
authors.initials: D
authors.lastname: Reed
authors.forename: Baruch S
authors.initials: BS
authors.lastname: Blumberg
authors.forename: Catharine A
authors.initials: CA
authors.lastname: Conley
mesh_terms: D000054:Acceleration / Q000009:adverse effects; D000818:Animals; D001272:Atmosphere; D017173:Caenorhabditis elegans / Q000254:growth & development; D003470:Culture Media; D005856:Germ-Free Life; D006358:Hot Temperature / Q000009:adverse effects; D008021:Life Support Systems; D019064:Product Packaging; D013026:Space Flight
chemical_list: D003470:Culture Media