[Study on the collecting method of constituents absorbed into the blood from anti-V capsule].
OBJECTIVE
Aim To establish the condition and parameters for collecting the effective constituents of Anti-V capsule.


METHODS
Based on HPLC, using chlorogenic acid and baicalin which are the main constituents absorbed in the blood as the marker substance, the best technical process of macropore absorbed resin was evaluated by the quantity of two compounds in the collected part.


RESULTS
After the abstraction of the capsule, the collection method of NKA-2 macropore absorbed resin as sorbent and 5 bed volume 50% ethanol as mobile phase was definited as the best craftwork. In the collected part, the content of chlorogenic acid and baicalin was five times than in the capsule.


CONCLUSIONS
In guide of serum pharmacochemistry, macropore absorbed resin is the effective method to collect the constituents absorbed in blood of Anti-V capsule. At the same time, this method provides a way to research other Compound medicine.
Wang
Xi-jun
XJ
Pharmacy Department, Heilongjiang University of Chinese Medicine, Harbin 150040, China. xijunw@sina.com
Zhang
Ningi
N
Sun
Hui
H
Cao
Hong-xin
HX
Sun
Wen-jun
WJ
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification; D002214:Capsules; D002726:Chlorogenic Acid / Q000097:blood / Q000302:isolation & purification*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005260:Female; D005419:Flavonoids / Q000097:blood / Q000302:isolation & purification*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012117:Resins, Synthetic; D013678:Technology, Pharmaceutical / Q000379:methods*
D000998:Antiviral Agents; D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012117:Resins, Synthetic; D002726:Chlorogenic Acid; C038044:baicalin