Changes in microRNA expression profiles in HIV-1-transfected human cells.
MicroRNAs (miRNAs) are small RNAs of 18-25 nucleotides (nt) in length that play important roles in regulating a variety of biological processes. Recent studies suggest that cellular miRNAs may serve to control the replication of viruses in cells. If such is the case, viruses might be expected to evolve the ability to modulate the expression of cellular miRNAs. To ask if expression of HIV-1 genes changes the miRNA profiles in human cells, we employed a high throughput microarray method, termed the RNA-primed Array-based Klenow Enzyme (RAKE) assay. Here, we describe the optimization of this assay to quantify the expression of miRNAs in HIV-1 transfected human cells. We report distinct differences in miRNA profiles in mock-transfected HeLa cells versus HeLa cells transfected with an infectious HIV-1 molecular clone, pNL4-3.
Yeung
Man Lung
ML
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health Bethesda, Maryland 20892-0460, USA. yeungm@niaid.nih.gov
Bennasser
Yamina
Y
Myers
Timothy G
TG
Jiang
Guojian
G
Benkirane
Monsef
M
Jeang
Kuan-Teh
KT
D020869:Gene Expression Profiling; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D016133:Polymerase Chain Reaction; D014162:Transfection
D035683:MicroRNAs