The LRR and Ig domain-containing membrane protein SST273 is expressed on motoneurons.
We performed signal sequence trap cDNA cloning using a cDNA library of enriched chicken embryonic spinal motoneurons and identified a novel transmembrane molecule, SST273 (Signal Sequence Trap clone 273). SST273 has five leucine-rich repeats (LRRs) and one immunoglobulin (Ig) domain in its extracellular domain. The human (KIAA1465) and mouse (BC059068) homologues of SST273 were already cloned, but had not yet been analyzed. The amino acid homologies between chick SST273 and human or mouse homologue are 52.1 and 51.5%, respectively. SST273 mRNA and its protein product were uniquely expressed in the embryonic spinal and cranial motoneurons at early developmental stages.
Gejima
Ryu
R
Division of Developmental Neurobiology and 21st Century COE, Kumamoto University Graduate School of Medical Sciences, Honjo1-1-1, Kumamoto 860-8556, Japan.
Okafuji
Tatsuya
T
Tanaka
Hideaki
H
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015153:Blotting, Western; D019556:COS Cells; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D015723:Gene Library; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry*; D007930:Leucine / Q000032:analysis*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000737:chemistry / Q000378:metabolism*; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000737:chemistry / Q000196:embryology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D008565:Membrane Proteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D007930:Leucine