title: Phylogenetic relationships of Brazilian bovine respiratory syncytial virus isolates and molecular homology modeling of attachment glycoprotein.
abstract: Bovine respiratory syncytial virus (BRSV) causes lower respiratory tract disease in young cattle. Recently, it was possible to determine the sequence of the G protein gene, which plays a role in the attachment of BRSV particles to the cells, from three distinct Brazilian isolates. The phylogenetic analysis conducted here using those sequences compared to other worldwide distributed isolates of BRSV allow us to allocate Brazilian strains within the subgroup B, which was no longer found in the world since the 1970s. One of the Brazilian strains has a major mutation between amino acid residues 173 and 178, within the central hydrophobic conserved region, exactly on the site of two of the four cysteine-noose forming cysteine residues. Homology modeling with the previously determined NMR structure of this protein domain was made to check whether these mutations altered the three-dimensional conformation of this immunodominant region. Possible consequences on the biological effects induced by such mutation on the G protein are discussed.
authors.affiliation: Laboratório de Virologia Animal, Instituto de Biologia, UNICAMP, P.O. Box 6109, 13084-970 Campinas, Brazil.
authors.forename: Fernando Rosado
authors.initials: FR
authors.lastname: Spilki
authors.forename: Renata Servan
authors.initials: RS
authors.lastname: Almeida
authors.forename: Helena Gallichio
authors.initials: HG
authors.lastname: Domingues
authors.forename: Regina Celia Freitas
authors.initials: RC
authors.lastname: D'Arce
authors.forename: Helena Lage
authors.initials: HL
authors.lastname: Ferreira
authors.forename: Jaqueline
authors.initials: J
authors.lastname: Campalans
authors.forename: Sandra Cecília Botelho
authors.initials: SC
authors.lastname: Costa
authors.forename: Clarice Weis
authors.initials: CW
authors.lastname: Arns
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001938:Brazil; D002417:Cattle; D019295:Computational Biology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D018357:Respiratory Syncytial Virus Infections / Q000662:veterinary / Q000821:virology; D018112:Respiratory Syncytial Virus, Bovine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics*
chemical_list: C066028:G protein, Bovine respiratory syncytial virus; D014759:Viral Envelope Proteins