High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel.
Simple and reliable thin-layer chromatography-densitometry methods for determination of antimycotics (bifonazole, clotrimazole, and miconazole) and preservatives (benzyl alcohol and benzoic acid) were developed. The pairs bifonazole/benzyl alcohol, clotrimazole/benzyl alcohol, and miconazole/benzoic acid were determined simultaneously. The following mobile phases were used: ethyl acetate-n-heptane-methanoldiethylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for bifonazole and benzyl alcohol; n-butyl acetate-n-heptane-methanol-dietylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for clotrimazole and benzyl alcohol; and n-butyl acetate-carbon tetrachloride-methanol-diethylamine (3 + 6 + 2.5 + 0.5, v/v/v/v) for miconazole and benzoic acid. The chromatographic zones on silica gel plates were scanned in the reflectance/absorbance mode at 230 nm (bifonazole, benzyl alcohol, miconazole, and benzoic acid) and 210 nm (clotrimazole and benzyl alcohol). The recovery for all substances ranged from 98.7 to 100.7%. The limits of detection and quantitation were 0.03 to 0.2 microg and 0.1 to 0.5 microg/spot, respectively. The proposed methods were applied for determination of antimycotics and preservatives in commercially available pharmaceuticals.
Cakar
Mira
M
University of Belgrade, Faculty of Pharmacy, Department of General and Inorganic Chemistry, Vojvode Stepe 450, P.O. Box 146, 11000 Belgrade, Serbia and Montenegro.
Popović
Gordana
G
Agbaba
Danica
D
D019817:Benzoic Acid / Q000032:analysis; D019905:Benzyl Alcohol / Q000032:analysis; D002138:Calibration; D002855:Chromatography, Thin Layer / Q000379:methods*; D005782:Gels / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007093:Imidazoles / Q000032:analysis*; D009824:Ointments / Q000032:analysis* / Q000737:chemistry; D011310:Preservatives, Pharmaceutical / Q000032:analysis*; D012015:Reference Standards; D012680:Sensitivity and Specificity
D005782:Gels; D007093:Imidazoles; D009824:Ointments; D011310:Preservatives, Pharmaceutical; D019817:Benzoic Acid; D019905:Benzyl Alcohol