Quantification of subendocardial and subepicardial blood flow using 15O-labeled water and PET: experimental validation.
The purpose of this study was to assess the feasibility and accuracy of quantifying subendocardial and subepicardial myocardial blood flow (MBF) and the relative coronary flow reserves (CFR) using (15)O-labeled water (H(2)(15)O) and 3-dimensional-only PET.


METHODS
Eight pigs were scanned with H(2)(15)O and (15)O-labeled carbon monoxide (C(15)O) after partially occluding the circumflex (n = 3) or the left anterior descending (n = 5) coronary artery, both at rest and during hyperemia induced by intravenous dipyridamole. Radioactive microspheres were injected during each of the H(2)(15)O scans.


RESULTS
In a total of 256 paired measurements of MBF, ranging from 0.30 to 4.46 mL.g(-1).min(-1), microsphere and PET MBF were fairly well correlated. The mean difference between the 2 methods was -0.01 +/- 0.52 mL.g(-1).min(-1) with 95% of the differences lying between the limits of agreement of -1.02 and 1.01 mL.g(-1).min(-1). CFR was significantly reduced (P < 0.05) in the ischemic subendocardium (PET = 1.12 +/- 0.45; microspheres = 1.09 +/- 0.50; P = 0.86) and subepicardium (PET = 1.2 +/- 0.35; microspheres = 1.32 +/- 0.5; P = 0.39) in comparison with remote subendocardium (PET = 1.7 +/- 0.62; microspheres = 1.64 +/- 0.61; P = 0.68) and subepicardium (PET = 1.79 +/- 0.73; microspheres = 2.19 +/- 0.86; P = 0.06).


CONCLUSIONS
Dynamic measurements using H(2)(15)O and a 3-dimensional-only PET tomograph allow regional estimates of the transmural distribution of MBF over a wide flow range, although transmural flow differences were underestimated because of the partial-volume effect. PET subendocardial and subepicardial CFR were in good agreement with the microsphere values.
Rimoldi
Ornella
O
Medical Research Council Clinical Sciences Centre, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Hammersmith Hospital, London, United Kingdom. ornella.rimoldi@csc.mrc.ac.uk
Schäfers
Klaus P
KP
Boellaard
Ronald
R
Turkheimer
Federico
F
Stegger
Lars
L
Law
Marilyn P
MP
Lammerstma
Adriaan A
AA
Camici
Paolo G
PG
D000465:Algorithms; D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D003326:Coronary Circulation / Q000502:physiology*; D004699:Endocardium / Q000000981:diagnostic imaging*; D005240:Feasibility Studies; D006352:Heart Ventricles / Q000000981:diagnostic imaging; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D010104:Oxygen Radioisotopes; D010496:Pericardium / Q000000981:diagnostic imaging*; D049268:Positron-Emission Tomography / Q000379:methods; D019275:Radiopharmaceuticals; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D014867:Water
D010104:Oxygen Radioisotopes; D019275:Radiopharmaceuticals; D014867:Water