title: Induction of neurite outgrowth by (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol from PC12 cells.
abstract: A lignan derivative, (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol (DHDA), was isolated from Kalopanax septemlobus L. and was observed to have neuritogenic activity. DHDA at 50 microM caused a marked induction of neurite outgrowth and an enhancement of nerve growth factor (NGF)-mediated neurite outgrowth from PC12 cells. However, it did not exhibit any neurotrophic action. At 50 microM, DHDA enhanced NGF-induced neurite-bearing activity. This activity was partially blocked by the mitogen-activated protein kinase (MAPK) inhibitor PD98059 and by GF109203X, a protein kinase C (PKC) inhibitor. These results suggest that DHDA can induce neurite outgrowth and enhance NGF-induced neurite outgrowth from PC12 cells by amplifying up-stream steps such as MAPK and PKC.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Korea.
authors.forename: Jung Soo
authors.initials: JS
authors.lastname: Shin
authors.forename: Yu Mi
authors.initials: YM
authors.lastname: Kim
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Hong
authors.forename: Ho Sang
authors.initials: HS
authors.lastname: Kang
authors.forename: Yoo Jung
authors.initials: YJ
authors.lastname: Yang
authors.forename: Don Koo
authors.initials: DK
authors.lastname: Lee
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
authors.forename: Jai Seup
authors.initials: JS
authors.lastname: Ro
authors.forename: Myung Koo
authors.initials: MK
authors.lastname: Lee
mesh_terms: D000818:Animals; D027481:Araliaceae; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D008031:Lignin / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008301:Maleimides / Q000494:pharmacology; D008518:Medicine, East Asian Traditional; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems; D010946:Plants, Medicinal; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats
chemical_list: D004791:Enzyme Inhibitors; D005419:Flavonoids; D007211:Indoles; D008301:Maleimides; C489718:pinonesinol; D008031:Lignin; D020932:Nerve Growth Factor; D011493:Protein Kinase C; D020929:Mitogen-Activated Protein Kinase Kinases; C070515:bisindolylmaleimide I; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one