Modulation of ERK and JNK activity by transient forebrain ischemia in rats.
The mitogen-activated protein (MAP) kinase families of ERK and JNK participate in numerous intracellular signaling pathways and are abundantly expressed in the CNS. Activation of ERK and JNK during reperfusion of ischemic tissue is implicated in promoting cell death, insofar as inhibition of either pathway reduces neuronal cell death. However, ERK or JNK activation provides protection in other neuronal injury models. In this study, we monitored the concurrent modulation of ERK and JNK activity in the hippocampus, neocortex, and striatum during ischemia and immediately upon reperfusion in a rat model of transient global ischemia. All three regions incur a similar reduction in blood flow during occlusion but show different extents and temporal patterns of injury following reperfusion. ERK and JNK were active in the normal rat forebrain, and phosphorylation was reduced by ischemia. Upon reperfusion, ERK was rapidly activated in the hippocampus, neocortex, and striatum, whereas JNK phosphorylation increased in the hippocampus and striatum but not in the neocortex. The response of JNK vs. ERK more closely reflects the susceptibility of these regions. JNK1 was the predominant phosphorylated isoform. A minor pool of phosphorylated JNK3 increased above the control level after reperfusion in hippocampal but not in neocortical particulate fractions. In addition, a novel 32-35-kDa c-Jun kinase activity was detected in the hippocampus, neocortex, and striatum. The results show that ERK and JNK activities are rapidly, but not identically, modulated by ischemia and reperfusion and indicate that the MAP kinase pathways contribute to regulating the response to acute CNS injury.
Shackelford
Deborah A
DA
Department of Neurosciences, University of California at San Diego, La Jolla, CA 92093-0624, USA. dshackelford@ucsd.edu
Yeh
Richard Y
RY
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D003600:Cytosol / Q000378:metabolism; D004195:Disease Models, Animal; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000502:physiology; D002546:Ischemic Attack, Transient / Q000201:enzymology* / Q000473:pathology; D048670:MAP Kinase Kinase 4 / Q000378:metabolism*; D009474:Neurons / Q000166:cytology; D010766:Phosphorylation; D016548:Prosencephalon / Q000201:enzymology* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion / Q000379:methods; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors
D048049:Extracellular Signal-Regulated MAP Kinases; D048670:MAP Kinase Kinase 4