title: Epithelial-specific transcription factor ESE-3 is involved in the development of monocyte-derived DCs.
abstract: Dendritic cells (DCs) are recognized as the most potent antigen-presenting cells of the immune system with the unique ability to initiate and maintain primary immune responses. In order to better characterize the functional and phenotypic features of DCs, a subtractive cDNA library to identify differentially expressed genes in monocyte-derived DCs (MDCs) was constructed. Using this approach, we found that the epithelial transcription factor ESE-3, which was previously shown to be exclusively expressed in cells of epithelial origin, is differentially expressed in MDCs. This was further confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analyses. The expression of ESE-3 is up-regulated upon maturation of MDCs and inhibited by treating the cells with IL-10 or IFN-gamma. Knockdown experiments using siRNA suggest that ESE-3 plays an important role during MDC development. Our results might help to improve the phenotypic characterization of DCs and lead to a better understanding of the cellular mechanisms involved in antigen presentation and T-cell stimulation.
authors.affiliation: Department of Hematology, Oncology and Immunology, University of Tübingen, Otfried-Müller Str 10, D-72076 Tübingen, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Appel
authors.forename: Anita
authors.initials: A
authors.lastname: Bringmann
authors.forename: Frank
authors.initials: F
authors.lastname: Grünebach
authors.forename: Markus M
authors.initials: MM
authors.lastname: Weck
authors.forename: Jürgen
authors.initials: J
authors.lastname: Bauer
authors.forename: Peter
authors.initials: P
authors.lastname: Brossart
mesh_terms: D017951:Antigen Presentation / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology*; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D009928:Organ Specificity / Q000235:genetics / Q000276:immunology; D034741:RNA, Small Interfering / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology*
chemical_list: C403306:EHF protein, human; D034741:RNA, Small Interfering; D014157:Transcription Factors