Effect of calcitonin gene-related peptide on anastomotic healing in the presence of endotoxin.
BACKGROUND
Intestinal anastomotic healing is a complex procedure in which several mediators, cytokines and other substances play roles, as well as calcitonin gene-related peptide (CGRP). CGRP is capable of stimulating DNA synthesis and cell proliferation in endothelial cells by increasing vasodilatation and inflammatory response and promoting epithelial, vascular and mesothelial cell proliferation. This study was undertaken to investigate whether CGRP has a beneficial effect on intestinal anastomotic healing, even in septic conditions.


METHODS
Four groups of 10 rats were administered normal saline (0.5 mL), lipopolysaccharide (LPS) (0.5 mg/kg), CGRP (0.5 mL 6.5 x 10(-10) mol/L) and LPS + CGRP (0.5 mg/kg + 0.5 mL 6.5 x 10(-10) mol/L) via intraperitoneal route, respectively, 24 h prior to operation and postoperatively. All rats underwent ileo-ileal end-to-end anastomosis. Anastomotic bursting pressure and tissue hydroxyproline levels were measured on postoperative day 7.


RESULTS
Calcitonin gene-related peptide was found to have positive effects on both parameters of healing. The LPS-injected group showed intestinal anastomotic healing disorder suggesting impaired collagen production, which showed improvement after CGRP administration.


CONCLUSIONS
Calcitonin gene-related peptide increases anastomotic wound healing in experimental intestinal anastomosis in the presence of endotoxin.
Dogmuş
Mehmet
M
Department of Surgery, School of Medicine, Gazi University, Ankara, Turkey.
Ersoy
Emin
E
Anadol
A Ziya
AZ
Ozel
Ummühani
U
Bilgehan
Ayşe
A
D000714:Anastomosis, Surgical; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology / Q000502:physiology; D005260:Female; D006909:Hydroxyproline / Q000032:analysis; D008297:Male; D051381:Rats; D013718:Tensile Strength / Q000187:drug effects; D014945:Wound Healing / Q000502:physiology*
D015740:Calcitonin Gene-Related Peptide; D006909:Hydroxyproline