New host plants of Erwinia amylovora in Bulgaria.
Nine strains of Erwinia amylovora were isolated from new host plants in Bulgaria--chokeberry and strawberry. The strains were characterized morphologically and biochemically using the API 20E and BIOLOG system. It was established that they showed three different API 20E metabolic profiles, not found by previous studies of E. amylovora. All strains were identified as E. amylovora due to their metabolic fingerprint patterns obtained by the BIOLOG system. The identification was confirmed by PCR amplification of a specific region of plasmid pEA29 and genome ams-region. This study is the first characterization and identification of E. amylovora strains isolated from chokeberry and strawberry by the API 20E and BIOLOG system and by polymerase chain reaction.
Atanasova
Iliana
I
Department of General and Industrial Microbiology, Faculty of Biology, The Sofia University, Bulgaria.
Kabadjova
Petia
P
Bogatzevska
Nevena
N
Moncheva
Penka
P
D002031:Bulgaria; D017931:DNA Primers; D044084:Erwinia amylovora / Q000145:classification / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D031985:Fragaria / Q000382:microbiology*; D005843:Geography; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D027861:Prunus / Q000382:microbiology*
D017931:DNA Primers