Heterologous expression of an Entamoeba histolytica chitin synthase in Saccharomyces cerevisiae.
Chitin in the cyst wall of Entamoeba histolytica is made by two chitin synthases (Chs), one of which is unique (EhCHS-1) and one of which resembles those of insects and nematodes (EhCHS-2). EhCHS-1 is deposited chitin in the lateral wall of transformed Saccharomyces cerevisiae Chs mutants, independent of accessory proteins (Chs4p to Chs7p) required by yeast Chs3p.
Van Dellen
Katrina L
KL
Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 715 Albany St., Evans 426, Boston, Massachusetts 02118, USA.
Bulik
Dorota A
DA
Specht
Charles A
CA
Robbins
Phillips W
PW
Samuelson
John C
JC
D000818:Animals; D002384:Catalysis; D002686:Chitin / Q000378:metabolism; D002687:Chitin Synthase / Q000235:genetics* / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression; D009154:Mutation / Q000235:genetics; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D002686:Chitin; D002687:Chitin Synthase