E. coli virulence factors in children with neurogenic bladder associated with bacteriuria.
The value of E. coli virulence factors in patients with neurogenic bladder has not been established. The aim of this study is to correlate E. coli virulence factors with asymptomatic and symptomatic UTI in children with neurogenic bladder. Fifty E. coli strains, which were collected in sequence, underwent analysis in relation to: the association to pyuria, serotype (O:H), the presence of genes and expression of fimbriae P, type 1, S and hemagglutinin Dr, the presence of the gene and production of hemolysins and cytotoxins (CNF1). We also analyzed the cell adherence capability and pattern and presence of usp (uropathogenic-specific protein). Pyuria was present in most of the positive urine cultures, with 86% AB and 97% UTI. Low rates of uropathogenic strains were observed in the two groups, with 18% AB and 21% UTI. Type 1 fimbria predominated in 44% of the E. coli strains. Of the bacteria studied, 30% (15 strains) exhibited papG genotypes (11 class II and 4 class III). Of these, 12/15 patients presented AB. Production of hemolysins was detected in 38% of the strains (16 AB and 3 UTI) and usp in only 18% of the strains, with 8 AB and 1 UTI. Adherence tests demonstrated the adhesive capacity in all samples analyzed. Neither group (AB or symptomatic UTI) presented a statistically significant difference in relation to the virulence factors studied. E. coli clones that caused symptomatic UTI in children with neurogenic bladder expressed few virulence factors, with no statistically significant difference in comparison to the AB group.
Guidoni
Eliana B M
EB
Department of Pediatrics Nephrology Unit, Irmandade da Santa Casa de Misericórdia de São Paulo, Brazil.
Dalpra
Vanessa A
VA
Figueiredo
Patrícia Maria
PM
da Silva Leite
Domingos
D
Mímica
Lycia Mara J
LM
Yano
Tomomasa
T
Blanco
Jesus E
JE
Toporovski
Julio
J
D001422:Bacterial Adhesion; D001437:Bacteriuria / Q000382:microbiology*; D002648:Child; D003603:Cytotoxins / Q000378:metabolism; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000150:complications / Q000382:microbiology*; D005260:Female; D010861:Fimbriae, Bacterial / Q000235:genetics; D006388:Hemagglutinins / Q000378:metabolism; D006460:Hemolysin Proteins / Q000378:metabolism; D006801:Humans; D008297:Male; D001750:Urinary Bladder, Neurogenic / Q000150:complications / Q000382:microbiology* / Q000652:urine; D014552:Urinary Tract Infections / Q000150:complications / Q000382:microbiology* / Q000652:urine; D037521:Virulence Factors / Q000378:metabolism*
D003603:Cytotoxins; D006388:Hemagglutinins; D006460:Hemolysin Proteins; D037521:Virulence Factors