title: The Structural Biology Center 19ID undulator beamline: facility specifications and protein crystallographic results.
abstract: The 19ID undulator beamline of the Structure Biology Center has been designed and built to take full advantage of the high flux, brilliance and quality of X-ray beams delivered by the Advanced Photon Source. The beamline optics are capable of delivering monochromatic X-rays with photon energies from 3.5 to 20 keV (3.5-0.6 A wavelength) with fluxes up to 8-18 x 10(12) photons s(-1) (depending on photon energy) onto cryogenically cooled crystal samples. The size of the beam (full width at half-maximum) at the sample position can be varied from 2.2 mm x 1.0 mm (horizontal x vertical, unfocused) to 0.083 mm x 0.020 mm in its fully focused configuration. Specimen-to-detector distances of between 100 mm and 1500 mm can be used. The high flexibility, inherent in the design of the optics, coupled with a kappa-geometry goniometer and beamline control software allows optimal strategies to be adopted in protein crystallographic experiments, thus maximizing the chances of their success. A large-area mosaic 3 x 3 CCD detector allows high-quality diffraction data to be measured rapidly to the crystal diffraction limits. The beamline layout and the X-ray optical and endstation components are described in detail, and the results of representative crystallographic experiments are presented.
authors.affiliation: Structural Biology Center, Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: Gerd
authors.initials: G
authors.lastname: Rosenbaum
authors.forename: Randy W
authors.initials: RW
authors.lastname: Alkire
authors.forename: Gwyndaf
authors.initials: G
authors.lastname: Evans
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Rotella
authors.forename: Krzystof
authors.initials: K
authors.lastname: Lazarski
authors.forename: Rong Guang
authors.initials: RG
authors.lastname: Zhang
authors.forename: Stephan L
authors.initials: SL
authors.lastname: Ginell
authors.forename: Norma
authors.initials: N
authors.lastname: Duke
authors.forename: Istvan
authors.initials: I
authors.lastname: Naday
authors.forename: Jack
authors.initials: J
authors.lastname: Lazarz
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Molitsky
authors.forename: Lisa
authors.initials: L
authors.lastname: Keefe
authors.forename: John
authors.initials: J
authors.lastname: Gonczy
authors.forename: Larry
authors.initials: L
authors.lastname: Rock
authors.forename: Ruslan
authors.initials: R
authors.lastname: Sanishvili
authors.forename: Martin A
authors.initials: MA
authors.lastname: Walsh
authors.forename: Edwin
authors.initials: E
authors.lastname: Westbrook
authors.forename: Andrzej
authors.initials: A
authors.lastname: Joachimiak
mesh_terms: D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007087:Illinois; D008967:Molecular Biology / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics / Q000295:instrumentation*; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D017356:Synchrotrons / Q000295:instrumentation*; D014584:User-Computer Interface
chemical_list: D011506:Proteins