Hemoperfusion with an immobilized polymyxin B column reduces the blood level of neutrophil elastase.
BACKGROUND
We investigated whether direct hemoperfusion with an immobilized polymyxin B column (DHP with PMX) could reduce the blood level of neutrophil elastase.


METHODS
20 sepsis patients were enrolled in the study. DHP with PMX was performed twice within a 24-hour period. Neutrophil elastase was measured 7 times.


RESULTS
Neutrophil elastase was 468 +/- 75.1 microg/l, while it was 1,531 +/- 201.7 microg/l immediately after the first session, declined to 351 +/- 73.9 microg/l before the second session of DHP with PMX, and increased again to 599.3 +/- 112.7 microg/l immediately after the second session, 328 +/- 73.7 microg/l at 24 h, 264 +/- 39.3 microg/l at 48 h, and 230 +/- 36.1 microg/l at 72 h after DHP with PMX. The levels from 48 h onwards were significantly lower compared with that before treatment.


CONCLUSIONS
DHP with PMX has an overall effect that reduces circulating neutrophil elastase levels.
Kushi
Hidehiko
H
Department of Emergency and Critical Care Medicine, Nihon University School of Medicine, Tokyo, Japan. hkushi@med.nihon-u.ac.jp
Miki
Takahiro
T
Nakahara
Jun
J
Okamoto
Kazuhiko
K
Saito
Takeshi
T
Tanjoh
Katsuhisa
K
D000327:Adsorption; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006464:Hemoperfusion / Q000379:methods*; D006801:Humans; D019272:Leukocyte Elastase / Q000097:blood* / Q000737:chemistry*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D011112:Polymyxin B / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
D008567:Membranes, Artificial; D019272:Leukocyte Elastase; D011112:Polymyxin B