Penicillium coffeae, a new endophytic species isolated from a coffee plant and its phylogenetic relationship to P. fellutanum, P. thiersii and P. brocae based on parsimony analysis of multilocus DNA sequences.
Penicillium coffeae is described as a novel endophyte isolated from a Coffea arabica L. plant in Hawaii. The species is slow growing with short, vesiculate, monoverticillate conidiophores. Phylogenetic analysis using three loci shows that P. coffeae forms a strongly supported clade with P. fellutanum, P. charlesii, P. chermesinum, P. indicum, P. phoeniceum and P. brocae. Phenotypic ally these species are quite similar but can be distinguished. The EF-1alpha gene from P. fellutanum, P. charlesii, P. chermesinum and P. indicum lack introns, P. coffeae and P. phoeniceum have a previously unknown intron at codon 20 and P. brocae and P. thiersii isolates have a single intron at codon 26. The most parsimonious interpretation of intron changes on the strongly supported phylogenetic tree requires the gain of a novel intron at position 20 and loss of intron 26 to arrive at the current distribution of introns in this gene. This is one of only a few examples of intron gain in genes.
Peterson
Stephen W
SW
Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, USA. peterssw@ncaur.usda.gov
Vega
Fernando E
FE
Posada
Francisco
F
Nagai
Chifumi
C
D040503:Coffea / Q000382:microbiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D006254:Hawaii; D007438:Introns / Q000235:genetics; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010407:Penicillium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013172:Spores, Fungal / Q000166:cytology
D004271:DNA, Fungal; D020648:Peptide Elongation Factor 1