title: Genetic diversity among silkworm (Bombyx mori L., Lep., Bombycidae) germplasms revealed by microsatellites.
abstract: To determine genetic relationships among strains of silkworm, Bombyx mori L., 31 strains with different origins, number of generations per year, number of molts per generation, and morphological characters were studied using simple sequence repeat (SSR) markers. Twenty-six primer pairs flanking microsatellite sequences in the silkworm genome were assayed. All were polymorphic and unambiguously separated silkworm strains from each other. A total of 188 alleles were detected with a mean value of 7.2 alleles/locus (range 2-17). The average heterozygosity value for each SSR locus ranged from 0 to 0.60, and the highest one was 0.96 (Fl0516 in 4013). The mean polymorphism index content (PIC) was 0.66 (range 0.12-0.89). Unweighted pair group method with arithmetic means (UPGMA) cluster analysis of Nei's genetic distance grouped silkworm strains based on their origin. Seven major ecotypic silkworm groups were analyzed. Principal components analysis (PCA) for SSR data support their UPGMA clustering. The results indicated that SSR markers are an efficient tool for fingerprinting cultivars and conducting genetic-diversity studies in the silkworm.
authors.affiliation: Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, P. R. China.
authors.forename: Muwang
authors.initials: M
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Anying
authors.initials: A
authors.lastname: Xu
authors.forename: Xuexia
authors.initials: X
authors.lastname: Miao
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Hou
authors.forename: Pingjiang
authors.initials: P
authors.lastname: Sun
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yongping
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000483:Alleles; D000818:Animals; D012831:Bombyx / Q000145:classification* / Q000235:genetics*; D016000:Cluster Analysis; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D006579:Heterozygote; D018598:Minisatellite Repeats / Q000235:genetics*; D010802:Phylogeny
chemical_list: D005819:Genetic Markers