Electronic sorting and recovery of single live cells from microlitre sized samples.
Sorting and recovering specific live cells from samples containing less than a few thousand cells have become major hurdles in rare cell exploration such as stem cell research, cell therapy and cell based diagnostics. We describe here a new technology based on a microelectronic chip integrating an array of over 100,000 independent electrodes and sensors which allow individual and parallel single cell manipulation of up to 10,000 cells while maintaining viability and proliferation capabilities. Manipulation is carried out using dynamic dielectrophoretic traps controlled by an electronic interface. We also demonstrate the capabilities of the chip by sorting and recovering individual live fluorescent cells from an unlabeled population.
Fuchs
Alexandra B
AB
BioChip Lab/Laboratoire Biopuces - CEA, 17 rue des Martyrs, 38054, Grenoble cedex 9, France. alexandra.fuchs@cea.fr
Romani
Aldo
A
Freida
Delphine
D
Medoro
Gianni
G
Abonnenc
Mélanie
M
Altomare
Luigi
L
Chartier
Isabelle
I
Guergour
Dorra
D
Villiers
Christian
C
Marche
Patrice N
PN
Tartagni
Marco
M
Guerrieri
Roberto
R
Chatelain
Francois
F
Manaresi
Nicolo
N
D049109:Cell Proliferation; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D002470:Cell Survival; D046209:Electrophoresis, Microchip / Q000379:methods*; D018401:Sample Size