Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end- joining-deficient genetic background.
The akuA gene encoding the Ku70 component of the nonhomologous end-joining machinery was deleted in the opportunistic pathogen Aspergillus fumigatus. No obvious phenotype could be assessed for the corresponding mutant strain but relative frequencies of homologous recombination were increased as deduced from targeting the laccase-encoding abr2 gene.
Krappmann
Sven
S
Institute of Microbiology & Genetics, Department of Molecular Microbiology and Genetics, Grisebachstr. 8, D-37077 Göttingen, Germany. skrappm@gwdg.de
Sasse
Christoph
C
Braus
Gerhard H
GH
D034961:Antigens, Nuclear / Q000235:genetics*; D001232:Aspergillus fumigatus / Q000166:cytology / Q000235:genetics* / Q000472:pathogenicity; D004268:DNA-Binding Proteins / Q000235:genetics*; D017353:Gene Deletion; D018390:Gene Targeting / Q000379:methods*; D005800:Genes, Fungal / Q000235:genetics; D000072200:Ku Autoantigen; D011995:Recombination, Genetic / Q000235:genetics*
D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D000072200:Ku Autoantigen