Induction of neurite outgrowth by (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol from PC12 cells.
A lignan derivative, (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol (DHDA), was isolated from Kalopanax septemlobus L. and was observed to have neuritogenic activity. DHDA at 50 microM caused a marked induction of neurite outgrowth and an enhancement of nerve growth factor (NGF)-mediated neurite outgrowth from PC12 cells. However, it did not exhibit any neurotrophic action. At 50 microM, DHDA enhanced NGF-induced neurite-bearing activity. This activity was partially blocked by the mitogen-activated protein kinase (MAPK) inhibitor PD98059 and by GF109203X, a protein kinase C (PKC) inhibitor. These results suggest that DHDA can induce neurite outgrowth and enhance NGF-induced neurite outgrowth from PC12 cells by amplifying up-stream steps such as MAPK and PKC.
Shin
Jung Soo
JS
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Kim
Yu Mi
YM
Hong
Seong Su
SS
Kang
Ho Sang
HS
Yang
Yoo Jung
YJ
Lee
Don Koo
DK
Hwang
Bang Yeon
BY
Ro
Jai Seup
JS
Lee
Myung Koo
MK
D000818:Animals; D027481:Araliaceae; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D008031:Lignin / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008301:Maleimides / Q000494:pharmacology; D008518:Medicine, East Asian Traditional; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems; D010946:Plants, Medicinal; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats
D004791:Enzyme Inhibitors; D005419:Flavonoids; D007211:Indoles; D008301:Maleimides; C489718:pinonesinol; D008031:Lignin; D020932:Nerve Growth Factor; D011493:Protein Kinase C; D020929:Mitogen-Activated Protein Kinase Kinases; C070515:bisindolylmaleimide I; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one