Radiation driven collapse of protein crystals.
During coherent X-ray diffraction measurements on crystals of ferritin at room temperature using monochromatic undulator radiation from the Advanced Photon Source, a sudden lattice contraction was observed following a characteristic latent period and ultimately leading to the collapse of the crystal. The progression of this collapse is analysed using a two-state Hendricks-Teller model. It reveals that 55% of the layers collapse by 1.6% before the crystal completely stops diffracting.
Boutet
Sébastien
S
Department of Physics, University of Illinois, Urbana, IL 61801, USA.
Robinson
Ian K
IK
D003198:Computer Simulation; D003460:Crystallization / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D005293:Ferritins / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D008956:Models, Chemical; D046912:Multiprotein Complexes / Q000737:chemistry / Q000528:radiation effects / Q000648:ultrastructure; D011487:Protein Conformation / Q000528:radiation effects; D011829:Radiation Dosage; D014961:X-Ray Diffraction; D014965:X-Rays
D046912:Multiprotein Complexes; D005293:Ferritins