Expression of fibroblast growth factor 1 (FGF1) and FGF7 in mature follicles during the periovulatory period after GnRH in the cow.
The aim of this study was to evaluate the expression pattern of mRNA for fibroblast growth factor 1 (FGF1), FGF7, and their receptor variants (FGFR2IIIb) in time-defined follicle classes before LH surge, between LH surge and ovulation, and in the early corpus luteum (CL) in the cow. The ovaries were collected by transvaginal ovariectomy (n=5 cows/group), and the follicles (n=5, one follicle/cow) were classified into the following groups: before GnRH administration (before LH surge); 3-5 h after GnRH (during LH surge); 10 h after GnRH; 20 h after GnRH; 25 h after GnRH (periovulation), and early CL (Days 2-3). The mRNA expression was analyzed by quantitative real-time PCR (RotorGene 3000). The mRNA expression of FGF1 showed no significant differences in the follicle groups examined, but increased significantly at the early CL phase. A transient increase in FGF7 mRNA expression was observed 3-5 h after GnRH and again in the early CL phase. In contrast, the expression of FGFR2IIIb was constant throughout the period from the final growth of the follicle to early CL formation. The results of this study suggest that FGF1 and FGF7 may be involved differently in the process of follicle maturation and CL formation, which is strongly dependent on angiogenesis.
Berisha
Bajram
B
Institute of Physiology Weihenstephan, Technical University of Munich, Freising-Weihenstephan, Germany. physio@wzw.tum.de
Welter
Harald
H
Shimizu
Takashi
T
Miyamoto
Akio
A
Meyer
Heinrich H D
HH
Schams
Dieter
D
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D003338:Corpus Luteum / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism*; D051523:Fibroblast Growth Factor 7 / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D015233:Models, Statistical; D018919:Neovascularization, Physiologic; D006080:Ovarian Follicle / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D010060:Ovulation; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000378:metabolism*; D013997:Time Factors
D017931:DNA Primers; D012333:RNA, Messenger; D016220:Fibroblast Growth Factor 1; D051523:Fibroblast Growth Factor 7; D007987:Gonadotropin-Releasing Hormone; D012313:RNA; D051497:Receptor, Fibroblast Growth Factor, Type 2