Selective immunoclean-up followed by liquid chromatography for the monitoring of a biomarker of exposure to polycyclic aromatic hydrocarbons in urine at the ng l-1 level.
A selective clean-up procedure using an immunosorbent (IS) was developed for the trace-level determination, in water and urine samples, of 3-benzo(a)pyrene-glucuronide (3-BP-G), a biomarker of exposure to carcinogenic polycyclic aromatic hydrocarbons (PAHs). First, three sorbents used for the immobilization of antibodies were evaluated for their ability to limit the risk of non-specific interactions and to provide a high bonding density. The best sorbent, i.e. sepharose, was used for the immobilization of two different monoclonal antibodies. The most specific antibody for 3-BP-G was applied to the selective extraction from urine providing a clean extract, an easy and reliable quantification by comparison with a classical SPE process. The sensitivity of the fluorescence associated with the selectivity of the IS provides a limit of detection up to 1.2 ng l(-1) in urine for 3-BP-G.
Maisonnette
Carine
C
Laboratoire Environnement et Chimie Analytique, LECA, UMR CNRS 7121, Ecole Supérieure de Physique et Chimie Industrielles, ESPCI, 10, rue Vauquelin 75231 Paris Cedex 05, France.
Simon
Patrice
P
Hennion
Marie-Claire
MC
Pichon
Valérie
V
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001580:Benzopyrenes / Q000032:analysis / Q000737:chemistry; D015415:Biomarkers / Q000737:chemistry / Q000652:urine*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007163:Immunosorbent Techniques / Q000295:instrumentation; D015394:Molecular Structure; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000652:urine*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000906:Antibodies; D001580:Benzopyrenes; D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; C039301:benzo(a)pyrene-3-O-glucuronide