[Evaluation of the effects of glycosylation on in vivo survival of cold-storage human platelets by using rabbit model].
To study the effects of glycosylation on survival of cold-storage human platelets by using rabbit model. (51)Cr-labeling platelets were used to detect the platelet storage survival. The human platelets (2.0 x 10(12)/L) treated with 5 g/L uridine diphosphate galactose (UDP-Gal) were stored in 4 degrees C refrigeratory up to 10 days. The survival of human platelets in rabbits whose reticuloendothelial system was inhibited by the administration of ethyl palmitate was monitored in blood drawn at various times after the platelet transfusion. The results showed that the survival rate of platelets was significantly increased in cold-storage human platelets by UDP-Gal treatment. The survival rates of platelets at 2 hours after transfusion into rabbits in groups of fresh platelets group, UDP-Gal + cold platelets group and cold platelets group were (68.9 +/- 8.5)%, (65.4 +/- 8.0)% and (5.0 +/- 2.6)%, respectively. Compared with cold platelets group, significant differences were seen among all groups (P < 0.01). UDP-Gal + cold platelets group had no significant differences compared with fresh platelets group (P > 0.05). It is concluded that UDG-Gal can provide the protective effect on cold-storage human platelets and prolong the survival time of refrigerated human platelets in rabbit model.
Tang
Rong-Cai
RC
Blood Center of Jiangsu Province, Nanjing 210042, China. xyzx@jswst.gov.cn
Huang
Cheng-Yin
CY
Pei
Xiao-Ping
XP
Chen
Bao-An
BA
Li
Cui-Ping
CP
Shi
Guang-Yao
GY
D000818:Animals; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D001793:Blood Preservation; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D023421:Models, Animal; D017713:Platelet Transfusion; D011817:Rabbits; D014531:Uridine Diphosphate Galactose / Q000494:pharmacology
D014531:Uridine Diphosphate Galactose