title: Influence of vitamin C and E supplementation on oxidative stress induced by hyperbaric oxygen in healthy men.
abstract: OBJECTIVE
  To investigate the effect of a 4-week vitamin C and E supplementation on oxidative stress induced by hyperbaric oxygen (HBO).
  
  
  METHODS
  19 healthy men were exposed to 3 sequential protocols, i.e. HBO (100% O2, 2.4 bar, 131 min) before (T1) and after 4 weeks of daily supplementation with 500 mg slow-release vitamin C and 272 IU vitamin E (T2). A normoatmospheric protocol (21% O2, 1.0 bar, 131 min) served as control treatment (nonexposed). Blood samples were taken before (B) and immediately after (A) treatment. Plasma levels of vitamin A, C, E, beta-carotene, reduced glutathione and malondialdehyde were measured by HPLC. Antioxidative capacity and lipid peroxides in plasma were analyzed by ELISA.
  
  
  RESULTS
  HBO decreased vitamin C and antioxidative capacity (T1). At T1, Delta A - B of vitamin C and lipid peroxides was different from nonexposed. Vitamin supplementation increased plasma levels of vitamin C and E by 28 and 37%, respectively. Vitamin supplementation led to decreased concentrations of lipid peroxides and reduced glutathione. After supplementation, HBO decreased vitamin C and reduced glutathione. At T2, Delta A - B of vitamin C and lipid peroxides was significantly different from nonexposed.
  
  
  CONCLUSIONS
  In humans, oxidative stress decreased plasma levels of vitamin C and antioxidative capacity and increased plasma lipid peroxides. Supplementation with vitamin C and E did not prevent these effects.
authors.affiliation: Institute of Human Nutrition and Food Science, Christian-Albrechts-Universitat zu Kiel, Kiel, Germany.
authors.forename: Nicolle
authors.initials: N
authors.lastname: Bader
authors.forename: Anja
authors.initials: A
authors.lastname: Bosy-Westphal
authors.forename: Andreas
authors.initials: A
authors.lastname: Koch
authors.forename: Manfred J
authors.initials: MJ
authors.lastname: Mueller
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000097:blood / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D018592:Cross-Over Studies; D019587:Dietary Supplements; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005978:Glutathione / Q000097:blood; D006801:Humans; D006931:Hyperbaric Oxygenation; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D014801:Vitamin A / Q000097:blood; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology*; D019207:beta Carotene / Q000097:blood
chemical_list: D000975:Antioxidants; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D008315:Malondialdehyde; D005978:Glutathione; D001205:Ascorbic Acid