Noninvasive measurement of cell volume changes by negative staining.
To maintain the intracellular concentration of ions and small molecules on osmotic challenges, nature has developed highly sophisticated transport systems for regulating water and ion content. An ideal measurement technique for volume changes of cells during osmotic challenges has to fulfil two requirements: it has to be osmotically inert, and it should allow online monitoring of cell volume changes. Here, a simple fluorescence microscopy-based approach is presented. Using fluorescein as a negative stain, it is possible to monitor cell volume changes without affecting the functionality of cell membranes and cell osmolarity. Measurement of Madine-Darby canine kidney (MDCK) cells after hypo- and hyperosmotic challenges reveals the main advantages of this approach: besides providing precise and reproducible quantitative data on reversible cell volume changes, the viability of the cells can be assessed directly by the appearance of stain in the cytoplasm. This becomes evident especially after hypo-osmotic challenge of glutaraldehyde-treated cells, which become leaky after fixation, followed by a massive volume change. This new approach represents a very sensitive measurement technique for cell volume changes resulting from water or ion flux, and thus seems to be an ideal tool for studying cell volume regulatory processes.
Droste
Miriam S
MS
Beiersdorf AG, Research Microscopy, Hamburg, Germany and FB Naturwissenschaftliche Technik, Hochschule für Angewandte Wissenschaften, Hamburg, Germany.
Biel
Stefan S
SS
Terstegen
Lara
L
Wittern
Klaus-Peter
KP
Wenck
Horst
H
Wepf
Roger
R
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D048429:Cell Size; D004285:Dogs; D005434:Flow Cytometry / Q000379:methods; D019793:Fluorescein; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007668:Kidney / Q000166:cytology* / Q000502:physiology*; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009997:Osmotic Pressure; D013194:Staining and Labeling / Q000379:methods; D014882:Water-Electrolyte Balance / Q000502:physiology*
D019793:Fluorescein