title: Cytotoxicity of a calcium aluminate cement in comparison with other dental cements and resin-based materials.
abstract: The objective of this study was to compare the cytotoxic effects of a calcium aluminate cement with several currently used direct restorative materials. Specimens of three composites (QuiXfil, Tetric Ceram, Filtek Supreme), one zinc phosphate cement (Harvard Cement), one glass ionomer cement (Ketac Molar), and one calcium aluminate cement (DoxaDent), were used fresh or after 7-days' preincubation in cell culture medium at 37 degrees C, pH 7.2. PVC strips for ISO 10993-5 cytotoxicity test were used as positive control and glass specimens as negative control. L-929 fibroblasts (5-ml aliquots, containing 3 x 10(4) cells/ml), cultivated in DMEM with 10% FCS, 1% glutamine, and 1% penicillin/streptomycin at 37 degrees C/5% CO2 and trypsinized, were exposed to the specimens for 72 h. The cells were harvested, centrifuged, and resuspended in 500 microl DMEM and then counted in 500 microl DMEM for 30 s with a flow cytometer at 488 nm. The analysis of variance comparing the six materials showed different influences on L-929 fibroblast cytotoxicity (p <0.0001). The cytotoxicity of all specimens diminished with increasing preincubation time (p <0.0001). Fresh DoxaDent exhibited the lowest cytotoxicity, followed by QuiXfil. Ketac Molar showed the highest cytotoxicity. After 7 days of preincubation, Harvard Cement and Filtek Supreme demonstrated more cytotoxicity than the other materials (p <0.005).
authors.affiliation: University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.
authors.forename: Alexander
authors.initials: A
authors.lastname: Franz
authors.forename: Katarina
authors.initials: K
authors.lastname: Konradsson
authors.forename: Franz
authors.initials: F
authors.lastname: König
authors.forename: Jan W V
authors.initials: JW
authors.lastname: Van Dijken
authors.forename: Andreas
authors.initials: A
authors.lastname: Schedle
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity*; D000704:Analysis of Variance; D000818:Animals; D017610:Calcium Compounds / Q000633:toxicity*; D003188:Composite Resins / Q000633:toxicity; D003738:Dental Cements / Q000633:toxicity*; D003793:Dental Restoration, Permanent / Q000379:methods; D005434:Flow Cytometry; D005899:Glass Ionomer Cements / Q000633:toxicity; D007739:L Cells / Q000187:drug effects; D051379:Mice; D019279:Resin Cements / Q000633:toxicity; D015036:Zinc Phosphate Cement / Q000633:toxicity
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D003188:Composite Resins; D003738:Dental Cements; C462459:DoxaDent; C474035:Filtek Supreme; D005899:Glass Ionomer Cements; C510379:QuiXfil; D019279:Resin Cements; C121818:Tetric ceram; C110712:ketac-molar; D015036:Zinc Phosphate Cement; C074226:Harvard Cement