Sequence variation in the Newcastle disease virus genome.
Full-length genome sequences of five virulent and five avirulent strains of Newcastle disease virus isolated between 1998 and 2002 in Victoria and New South Wales, Australia were determined. Comparisons between these strains revealed that coding sequence variability in the haemagglutinin-neuraminidase (HN), matrix (M) and phosphoprotein (P) gene sequences appeared to be more variable than in the fusion (F), nucleocapsid (N) and RNA dependent-RNA replicase (L) genes. Sequence analysis of a number of other isolates made during the recent virulent NDV outbreaks, also identified the presence of a number of variants with altered F gene cleavage sites, which resulted in altered biological properties of those viruses. Quasispecies analysis of a number of field isolates indicated the presence of virulent virus in one particular isolate. Gene sequence analysis of the progenitor virus isolated in 1998 showed very little sequence variation when compared to that of a progenitor-like virus isolated in 2001, demonstrating that in the field, viral genome sequence variation appears to be biologically restricted to that of a consensus sequence.
Kattenbelt
Jacqueline A
JA
CSIRO Livestock Industries, Australian Animal Health Laboratory, P.O. Bag 24, Geelong, Victoria 3220, Australia. j.kattenbelt@uq.edu.au
Stevens
Matthew P
MP
Gould
Allan R
AR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D014644:Genetic Variation; D016679:Genome, Viral; D015707:HN Protein / Q000235:genetics; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000235:genetics*; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017422:Sequence Analysis, DNA; D014760:Viral Fusion Proteins / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D015707:HN Protein; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; C055691:P phosphoprotein, Newcastle disease virus; D010750:Phosphoproteins; D012367:RNA, Viral; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C409209:nucleoprotein, Newcastle disease virus; D012324:RNA-Dependent RNA Polymerase