Salt enhances calmodulin-target interaction.
Calmodulin (CaM) operates as a Ca(2+) sensor and is known to interact with and regulate hundreds of proteins involved in a great many aspects of cellular function. It is of considerable interest to understand the balance of forces in complex formation of CaM with its target proteins. Here we have studied the importance of electrostatic interactions in the complex between CaM and a peptide derived from smooth-muscle myosin light-chain kinase by experimental methods and Monte Carlo simulations of electrostatic interactions. We show by Monte Carlo simulations that, in agreement with experimental data, the binding affinity between CaM and highly charged peptides is surprisingly insensitive to changes in the net charge of both the protein and peptide. We observe an increase in the binding affinity between oppositely charged partners with increasing salt concentration from zero to 100 mM, showing that formation of globular CaM-kinase type complexes is facilitated at physiological ionic strength. We conclude that ionic interactions in complex formation are optimized at pH and saline similar to the cell environment, which probably overrules the electrostatic repulsion between the negatively charged Ca(2+)-binding domains of CaM. We propose a conceivable rationalization of CaM electrostatics associated with interdomain repulsion.
André
Ingemar
I
Department of Biophysical Chemistry, Lund University, Chemical Centre, Lund, Sweden.
Kesvatera
Tõnu
T
Jönsson
Bo
B
Linse
Sara
S
D000818:Animals; D002147:Calmodulin / Q000737:chemistry* / Q000235:genetics; D002942:Circular Dichroism; D003198:Computer Simulation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D009010:Monte Carlo Method; D009154:Mutation; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry* / Q000235:genetics; D019906:Nuclear Magnetic Resonance, Biomolecular; D009994:Osmolar Concentration; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012492:Salts / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D055672:Static Electricity
D002147:Calmodulin; D010455:Peptides; D011994:Recombinant Proteins; D012492:Salts; D009219:Myosin-Light-Chain Kinase