Transformation of Actinidia eriantha: a potential species for functional genomics studies in Actinidia.
Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on lower axillary branches and the species flowers prolifically in greenhouse conditions. Flowering and fruiting of transgenic A. eriantha plants was obtained within 2 years of transformation in a containment greenhouse. GUS (beta-glucuronidase) activity indicating stable expression of the uidA gene was observed in leaf, stem, root, petal and fruit tissues. Molecular evidence for incorporation of transgenes into the A. eriantha genome was obtained by PCR and DNA gel blot analysis. Inheritance of transgenic phenotypes was demonstrated in seedling progeny. Functional genomic studies in kiwifruit have been initiated using transgenic A. eriantha plants.
Wang
Tianchi
T
The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand. tianchi.wang@hortresearch.co.nz
Ran
Yidong
Y
Atkinson
Ross G
RG
Gleave
Andrew P
AP
Cohen
Dan
D
D029042:Actinidia / Q000235:genetics* / Q000378:metabolism; D035264:Flowers / Q000235:genetics / Q000502:physiology; D005638:Fruit / Q000235:genetics / Q000502:physiology; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
D005966:Glucuronidase