[Effect of isorhmnetin on circadian rhythms of DNA synthesis and expression of c-myc gene in Eca-109 cells of human oesophageal cancer].
This study was focused on the circadian rhythms of DNA synthesis and the expression of c-myc gene in untreated and treated Eca-109 cells in human oesophageal cancer with isorhmnetin. The circadian rhythms of 3H-TdR incorporation and expression of c-myc gene in untreated and treated Eca-109 cells were measured by 3H-thymidine uptake assay and flow cytometry. The data collected were analyzed by ANOVA and Cosinor method. DNA synthesis and expression of c-myc gene in untreated group varied according to circadian time with statistical significance, the distribution curves of both DNA synthesis and the expression level of c-myc were fit for cosinor changes. The circadian rhythms of DNA synthesis and circadian parameters of c-myc expression in treated Eca-109 cells changed. The circadian parameters of DNA synthesis and expression level of c-myc varied after treatment by isorhmnetin. The effects of isorhmnetin on cell proliferation and c-myc expression reached the highest level from 20: 00 to 0: 00. The results provide a guidance for instituting the chemotherapy and chronotherapy of human tumors, when isorhmnetin is for use as anti-cancer agent.
Yang
Chunlei
C
Department of Medical Cell Biology, Life Science College, Sichun University, Chengdu 610041, China.
Peng
Tao
T
Qu
Yi
Y
Tao
Dachang
D
Wang
Zhengrong
Z
Zhu
Bin
B
D002940:Circadian Rhythm / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D044948:Flavonols / Q000494:pharmacology*; D006801:Humans; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis* / Q000235:genetics; D011794:Quercetin / Q000031:analogs & derivatives; D014407:Tumor Cells, Cultured
D044948:Flavonols; D016271:Proto-Oncogene Proteins c-myc; C047368:3-methylquercetin; D004247:DNA; D011794:Quercetin