Study of the experimental conditions for the lipase production by a newly isolated strain of Pseudomonas aeruginosa for the enantioselective hydrolysis of (+/-)-methyl trans-3(4-methoxyphenyl) glycidate.
A lipase producing bacterium has been isolated from the soil to enantiospecifically hydrolyze the (+/-)-methyl trans-3(4-methoxyphenyl) glycidate (MPGM), an intermediate in the synthesis of cardiovascular drug, diltiazem. This hydrolysis provided the desired (-)-MPGM in 44% yield with 99% enantiomeric excess. The organism was identified and confirmed as Pseudomonas aeruginosa by 16S rRNA sequencing. The various physiochemical parameters have been optimized for the maximum production of lipase in shake flask. Beef extract was found to be the best nitrogen source for lipase production. The optimized cultivation conditions were 30 degrees C with an initial medium pH 8 in shake flask. Both inoculum age and inoculum concentration have positive effect on the lipase production and (+/-)-MPGM (3 mM) was found to be the optimal inducer.
Singh
Sawraj
S
Biocatalysis Laboratory, Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research (NIPER), 160 062, S. A. S. Nagar, Punjab, India.
Kaur
Gurmeet
G
Chakraborti
Asit K
AK
Jain
Rakesh K
RK
Banerjee
Uttam C
UC
D004789:Enzyme Activation; D004852:Epoxy Compounds / Q000737:chemistry*; D006868:Hydrolysis; D007536:Isomerism; D008049:Lipase / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D011422:Propionates / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity
D004852:Epoxy Compounds; D011422:Propionates; C007869:glycidic acid; D008049:Lipase