Haemolytic assay for the detection of adenylate cyclase toxin of Bordetella pertussis.
The aim of this study was to device a simple, sensitive and convenient assay for the detection of toxicity of the Adenylate cyclase toxin (ACT), a newly recognized toxin of Bordetella pertussis. Haemolytic activity of ACT was analysed as an assay and sheep RBCs were found to be suitable to detect the toxic effect down to the dose of 6.25 nmol cAMP/min/ml enzymic activity applied. The haemolytic activity was found to be dose-dependent.
Kazi
Y F
YF
Department of Microbiology, Shah Abdul Latif University, Khairpur Mirs, Pakistan.
Parton
R
R
Memon
B A
BA