Effect of DSS-induced colitis on visceral sensitivity to colorectal distension in mice.
The present study aimed at evaluating the effect of dextran sodium sulphate (DSS)-induced colitis on visceral sensitivity, measured as the visceromotor response (VMR) to colorectal distension (CRD) in BALB/c and C57Bl/6 male mice. Inflammation was induced by the addition of 4% DSS to the drinking water for 5 (C57Bl/6) or 6-7 days (BALB/c). Parallel groups were used to monitor histopathological changes and visceral sensitivity. Pseudo-affective visceral pain responses were evoked using an increasing phasic CRD paradigm (10-60 mmHg) in conscious mice on predetermined days (pretreatment controls, 12, 16, 20, 30, 40 and 51). In both mouse strains, significant histopathological changes developed between days 2 and 5 of DSS treatment, and persisted until day 12 (P < 0.05). On day 15, inflammatory scores were reduced by about 50%. Despite evidence of inflammation in DSS-treated mice, no differences could be shown in the VMR to CRD between DSS-treated mice and controls at any time point tested. In addition, no differences were seen before and after DSS treatment in the same group of mice. In conclusion, these data suggest that DSS-induced colonic inflammation does not affect the visceral sensitivity to CRD, neither at short or long term, in BALB/c or C57Bl/6 male mice.
Larsson
M H
MH
Department of Integrative Pharmacology, Gastrointestinal Biology, AstraZeneca R&D Mölndal, Mölndal, Sweden. marie.h.larsson@astrazeneca.com
Rapp
L
L
Lindström
E
E
D000818:Animals; D000998:Antiviral Agents / Q000633:toxicity*; D003092:Colitis / Q000139:chemically induced / Q000150:complications* / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity*; D004106:Dilatation; D004576:Electromyography; D005769:Gastrointestinal Motility / Q000502:physiology; D006930:Hyperalgesia / Q000209:etiology / Q000503:physiopathology*; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D017288:Pain Threshold / Q000502:physiology*; D014781:Viscera / Q000187:drug effects / Q000502:physiology
D000998:Antiviral Agents; D016264:Dextran Sulfate