Exercise differentially regulates synaptic proteins associated to the function of BDNF.
We explored the capacity of exercise to impact select events comprising synaptic transmission under the direction of brain-derived neurotrophic factor (BDNF), which may be central to the events by which exercise potentiates synaptic function. We used a specific immunoadhesin chimera (TrkB-IgG) that mimics the BDNF receptor, TrkB, to selectively block BDNF in the hippocampus during 3 days of voluntary wheel running. We measured resultant synapsin I, synaptophysin, and syntaxin levels involved in vesicular pool formation, endocytosis, and exocytosis, respectively. Synapsin I is involved in vesicle pool formation and neurotransmitter release, synaptophysin, in the biogenesis of synaptic vesicles and budding, and syntaxin, in vesicle docking and fusion. Exercise preferentially increased synapsin I and synaptophysin levels, without affecting syntaxin. There was a positive correlation between synapsin I and synaptophysin in exercising rats and synapsin I with the amount of exercise. Blocking BDNF abrogated the exercise-induced increases in synapsin I and synatophysin, revealing that exercise regulates select properties of synaptic transmission under the direction of BDNF.
Vaynman
Shoshanna S
SS
Department of Physiological Science, UCLA, 621 Charles E. Young Drive, Los Angeles, CA 90095, USA.
Ying
Zhe
Z
Yin
Dali
D
Gomez-Pinilla
Fernando
F
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism; D007074:Immunoglobulin G / Q000235:genetics; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D013569:Synapses / Q000378:metabolism*; D016704:Synapsins / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology*; D013572:Synaptic Vesicles / Q000378:metabolism; D016708:Synaptophysin / Q000378:metabolism
D019208:Brain-Derived Neurotrophic Factor; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D016704:Synapsins; D016708:Synaptophysin; D020813:Receptor, trkB