title: Calpain-3 mutations in Turkey.
abstract: Autosomal recessive limb-girdle muscular dystrophies (LGMD2s) are a clinically and genetically heterogeneous group of disorders, characterized by progressive involvement of the proximal limb girdle muscles; the group includes at least 10 different genetic entities. The calpainopathies (LGMD2A), a subgroup of LGMD2s, are estimated to be the most common forms of LGMD2 in all populations so far investigated. LGMD2A is usually characterized by symmetrical and selective atrophy of pelvic, scapular and trunk muscles and a moderate to gross elevation of serum CK. However, the course is highly variable. It is caused by mutations in the CAPN3 gene, which encodes for the calpain-3 protein. Until now, 161 pathogenic mutations have been found in the CAPN3 gene. In the present study, through screening of 93 unrelated LGMD2 families, we identified 29 families with LGMD2A, 21 (22.6%) of which were identified as having CAPN3 gene mutations. We detected six novel (p.K211N, p.D230G, p.Y322H, p.R698S, p.Q738X, c.2257delGinsAA) and nine previously reported mutations (c.550delA, c.19_23del, c.1746-20C>G, p.R49H, p.R490Q, p.Y336N, p.A702V, p.Y537X, p.R541Q) in the CAPN3 gene. There may be a wide variety of mutations, but clustering of specific mutations (c.550delA: 40%, p.R490Q: 10%) could be used in the diagnostic scheme in Turkey.
authors.affiliation: Faculty of Medicine, Department of Medical Biology, Hacettepe University, 6th floor, (06100), Sihhiye, Ankara, Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Balci
authors.forename: Stefania
authors.initials: S
authors.lastname: Aurino
authors.forename: Göknur
authors.initials: G
authors.lastname: Haliloglu
authors.forename: Beril
authors.initials: B
authors.lastname: Talim
authors.forename: Sevim
authors.initials: S
authors.lastname: Erdem
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Akcören
authors.forename: Ersin
authors.initials: E
authors.lastname: Tan
authors.forename: Melda
authors.initials: M
authors.lastname: Caglar
authors.forename: Isabelle
authors.initials: I
authors.lastname: Richard
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Nigro
authors.forename: Haluk
authors.initials: H
authors.lastname: Topaloglu
authors.forename: Pervin
authors.initials: P
authors.lastname: Dincer
mesh_terms: D000293:Adolescent; D000328:Adult; D002154:Calpain / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D008040:Genetic Linkage; D005820:Genetic Testing; D006801:Humans; D009124:Muscle Proteins / Q000235:genetics*; D049288:Muscular Dystrophies, Limb-Girdle / Q000175:diagnosis / Q000235:genetics*; D009154:Mutation; D014421:Turkey
chemical_list: D009124:Muscle Proteins; C105884:CAPN3 protein, human; D002154:Calpain