title: Inorganic particles in the skin of inhabitants of volcanic areas of Central America: their possible immunomodulatory influence in leishmaniasis and leprosy.
abstract: We have evaluated biopsies from patients with atypical nodular and typical ulcerated lesions of cutaneous leishmaniasis, from leishmanin reactions and skin from normal individuals from Nicaragua, Honduras and Guatemala for the presence of inorganic particles using confocal microscopy with a polarised light source and conventional histopathological techniques. Analysis by semiquantitative confocal microscopy permitted the demonstration of significantly larger numbers of particles in atypical lesions. Silica and aluminium, important components of these particles, were less abundant in particles from normal skin. The histology of these atypical lesions, characterised by 'naked' sarcoidal granulomas with epithelioid differentiation but very few lymphocytes, was very similar to the histological reaction observed after 14 days in persisting inflammation at leishmanin skin test sites. The presence of these unusual lesions in areas of Central American countries characterised by the presence of large amounts of volcanic ash, as well the unexpectedly low prevalence of leprosy in Central America, suggest that environmental factors may contribute significantly to the frequency and clinical manifestations of these infections. Among possible environmental features, the presence of inorganic particles with immunomodulatory properties in the skin may be a significant factor.
authors.affiliation: Instituto de Biomedicina, Universidad Central de Venezuela/Ministerio de Salud y Desarrollo Social, Apartado 4043, Caracas 1010A, Venezuela. jconvi@telcel.net.ve
authors.forename: J
authors.initials: J
authors.lastname: Convit
authors.forename: M
authors.initials: M
authors.lastname: Ulrich
authors.forename: J
authors.initials: J
authors.lastname: Castillo
authors.forename: H
authors.initials: H
authors.lastname: De Lima
authors.forename: M
authors.initials: M
authors.lastname: Pérez
authors.forename: N
authors.initials: N
authors.lastname: Caballero
authors.forename: J
authors.initials: J
authors.lastname: Hung
authors.forename: B
authors.initials: B
authors.lastname: Arana
authors.forename: P
authors.initials: P
authors.lastname: Pérez
mesh_terms: D000535:Aluminum / Q000032:analysis; D002489:Central America / Q000453:epidemiology; D004781:Environmental Exposure / Q000009:adverse effects / Q000032:analysis; D005547:Foreign Bodies / Q000175:diagnosis* / Q000276:immunology; D015745:Granuloma, Foreign-Body / Q000209:etiology / Q000473:pathology; D006801:Humans; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology / Q000276:immunology*; D007918:Leprosy / Q000453:epidemiology / Q000276:immunology*; D012822:Silicon Dioxide / Q000032:analysis; D012867:Skin; D018707:Volcanic Eruptions / Q000009:adverse effects / Q000032:analysis
chemical_list: D012822:Silicon Dioxide; D000535:Aluminum