The first radiation hybrid map of a perch-like fish: the gilthead seabream (Sparus aurata L).
Among Teleosts, Perciformes are the largest order of fishes and include numerous species of commercial importance. Perciformes also comprise species of primary interest for evolutionary studies and analysis of the sex determination systems and sex chromosome plasticity. Unfortunately, genomics tools and resources for Perciformes remain to be developed. Here, we report the production of a seabream whole-genome radiation hybrid (RH) panel in which quality was ascertained by the construction of a 2-Mb-resolution RH map. The map encompasses 440 markers (288 microsatellites, 82 gene-based markers, and 70 STS) suitable for linkage analysis and comparative mapping studies. Achievement of a RH panel and a whole-genome RH map should contribute to establishing seabream as a fish model among the Perciformes and should be of importance in aquaculture for marker-assisted selection, improvement of growth performance, and disease management. Development of RH maps in a cost-effective manner for other fishes with the described methodology will offer a powerful approach in aquaculture and will provide extended capabilities for comparing vertebrate genome evolution.
Senger
Fabrice
F
CNRS UMR 6061 Génétique et Développement, Université de Rennes 1, Faculté de Médecine, 2 Avenue du Pr Léon Bernard, 35043 Rennes Cedex, France.
Priat
Catherine
C
Hitte
Christophe
C
Sarropoulou
Elena
E
Franch
Rafaella
R
Geisler
Robert
R
Bargelloni
Luca
L
Power
Deborah
D
Galibert
Francis
F
D000818:Animals; D005075:Biological Evolution; D005819:Genetic Markers; D066298:In Vitro Techniques; D008957:Models, Genetic; D010473:Perciformes / Q000145:classification / Q000235:genetics; D021101:Radiation Hybrid Mapping / Q000379:methods*; D021541:Sea Bream / Q000235:genetics*; D016324:Sequence Tagged Sites; D013045:Species Specificity
D005819:Genetic Markers