title: Analysis of protein redox modification by hypoxia.
abstract: We examined hypoxia-induced changes in global thiol proteome profile in human prostate cancer cells using a BIAM-based display method. We analyzed the kinetics of protein thiol modification by using a pattern recognition algorithm, self-organizing maps (SOM) clustering, and identified the BIAM-labeled proteins by MALDI-TOF and ESI-tandem mass spectrometry. We found 99 out of 215 of total BIAM-labeled proteins were affected by hypoxia treatment and, yet, with diverse patterns and kinetics of redox modification. Our study proved that proteomics analysis employing the BIAM-labeling method can provide valuable information pertaining to global changes in the redox status of proteins in response to hypoxia.
authors.affiliation: Department of Cellular Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
authors.forename: Kyoung-Soo
authors.initials: KS
authors.lastname: Choi
authors.forename: Soo-Yeon
authors.initials: SY
authors.lastname: Park
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Baek
authors.forename: Rama
authors.initials: R
authors.lastname: Dey-Rao
authors.forename: Young-Mee
authors.initials: YM
authors.lastname: Park
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhang
authors.forename: Clement
authors.initials: C
authors.lastname: Ip
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Park
authors.forename: Yeul Hong
authors.initials: YH
authors.lastname: Kim
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Park
mesh_terms: D017209:Apoptosis / Q000502:physiology; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D020169:Caspases / Q000378:metabolism; D015687:Cell Hypoxia / Q000502:physiology; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005029:Ethylenediamines / Q000737:chemistry; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D010084:Oxidation-Reduction; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005029:Ethylenediamines; C093128:N-biotinoyl-N'-iodoacetylethylenediamine; D011506:Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D001710:Biotin; D011065:Poly(ADP-ribose) Polymerases; D048031:JNK Mitogen-Activated Protein Kinases; D020169:Caspases