title: Regulation of sympathetic and parasympathetic nerve activities by BIT/SHPS-1.
abstract: The hypothalamus plays a central role in the homeostatic regulation of internal physiological conditions such as body temperature and energy balance. We have previously shown that cold exposure enhances tyrosine phosphorylation of BIT/SHPS-1 (brain immunoglobulin-like molecule with tyrosine-based activation motifs/SHP substrate-1) in hypothalamic nuclei including the suprachiasmatic nucleus. In order to elucidate the function of BIT/SHPS-1 in the hypothalamus, we stimulated BIT/SHPS-1 in vivo by using the anti-BIT monoclonal antibody (mAb) 1D4, which reacts with the extracellular domain of BIT/SHPS-1 and induces its tyrosine phosphorylation. Administration of mAb 1D4 into the third cerebral ventricle enhanced the electrical activity of the renal sympathetic nerves, while it suppressed that of the gastric parasympathetic nerves. Similarly, blood pressure increased in response to the mAb 1D4 injection, and additionally, temperatures of the abdomen and brown adipose tissue increased. These results indicate that BIT/SHPS-1 is involved in the hypothalamic regulation of thermogenesis via the autonomic nervous system.
authors.affiliation: Laboratory of Proteins Involved in Homeostatic Integration, Division of Integrated Protein Functions, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Mamoru
authors.initials: M
authors.lastname: Tanida
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Okumura
authors.forename: Juri
authors.initials: J
authors.lastname: Hamada
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Sano
authors.forename: Katsuya
authors.initials: K
authors.lastname: Nagai
mesh_terms: D000005:Abdomen; D002001:Adipose Tissue, Brown; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D001794:Blood Pressure; D001831:Body Temperature; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004594:Electrophysiology; D004622:Embryo, Mammalian / Q000166:cytology; D007031:Hypothalamus / Q000378:metabolism / Q000502:physiology; D007276:Injections, Intraventricular; D007668:Kidney / Q000294:innervation; D008297:Male; D010275:Parasympathetic Nervous System / Q000502:physiology*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D011971:Receptors, Immunologic / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D013270:Stomach / Q000294:innervation; D013564:Sympathetic Nervous System / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism; D014630:Vagus Nerve
chemical_list: D000911:Antibodies, Monoclonal; D011971:Receptors, Immunologic; C501924:Sirpa protein, rat; D014443:Tyrosine