title: [Preparation of monoclonal antibodies to the Fc-fragment of human IgG and the use of their based immunoenzyme conjugates].
abstract: An original kit containing 17 clones of hybridomas, the producers of monoclonal antibodies (MCAb) against the Fc-fragments of human IgG, has been obtained. The possibility of using horseradish peroxidase conjugates of obtained MCAb as a part of ELISA test systems (Diaproph-Med, Ukraine) was comparatively studied. The application of peroxidase conjugates of two monoclonal antibodies (156C10 and 153H11) as part of five diagnostic kits was shown to provide a high specificity and a high sensitivity.
authors.forename: I V
authors.initials: IV
authors.lastname: Nikolaenko
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Galkin
authors.forename: G E
authors.initials: GE
authors.lastname: Raevskaia
authors.forename: T V
authors.initials: TV
authors.lastname: Kas'ianenko
authors.forename: M I
authors.initials: MI
authors.lastname: Nereshchenko
authors.forename: E S
authors.initials: ES
authors.lastname: Donskaia
authors.forename: N Ia
authors.initials: NIa
authors.lastname: Spivak
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007141:Immunoglobulin Fc Fragments / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic; D006735:Horseradish Peroxidase