The mobilization of hematopoietic progenitors to peripheral blood is predictive of the hematopoietic syndrome after total or partial body irradiation of mice.
OBJECTIVE
In previous studies we showed that administration of mobilizing growth factors (MGFs) to mice previously exposed to total body irradiation mobilizes to peripheral blood (PB) a number of progenitors that correlates with the total reserve of progenitors surviving the exposure. Now we have tested whether this finding is independent of the radiosensitivity of the mice and of the homogeneity of the radiation exposure. Also we have investigated whether numbers of mobilized progenitors predict the hematopoietic syndrome after irradiation.


METHODS
Mice were subjected to partial or total body irradiation and treated with MGFs. Thereafter, the number of colony-forming units granulocyte-macrophage progenitors in PB was correlated with the total reserve of surviving progenitors and with the nadir of leukocytes after the irradiation.


RESULTS
The number of progenitors mobilized to PB after irradiation of normal and radiosensitive mice showed the same correlation with respect to the reserve of bone marrow progenitors surviving the exposure. Additionally, the number of mobilized progenitors correlated with the leukocytes' nadir after the irradiation, regardless of homogeneous or inhomogeneous exposures.


CONCLUSIONS
In a mouse experimental model, the number of hematopoietic progenitors mobilized to PB by MGFs is a good predictor of the hematopoietic syndrome occurring after total or partial body irradiation.
Grande
Teresa
T
Hematopoiesis and Gene Therapy Division, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas, Madrid, Spain.
Bueren
Juan A
JA
D000818:Animals; D001853:Bone Marrow / Q000737:chemistry; D002452:Cell Count; D002470:Cell Survival; D005260:Female; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000528:radiation effects*; D007958:Leukocyte Count; D007962:Leukocytes / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D011836:Radiation Tolerance; D011994:Recombinant Proteins; D013577:Syndrome; D013926:Thrombopoietin / Q000494:pharmacology; D014916:Whole-Body Irradiation
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013926:Thrombopoietin; D000069585:Filgrastim