Analysis of six new genes encoding lysis proteins and coat proteins in Escherichia coli group A RNA phages.
Group A RNA phages consist of four genes-maturation protein, coat protein, lysis protein and replicase genes. We analyzed six plasmids containing lysis protein genes and coat protein genes of Escherichia coli group A RNA phages and compared their amino acid sequences with the known proteins of E. coli(group A), Pseudomonas aeruginosa(PP7) RNA phages and Rg-lysis protein from Qbeta phage. The size of lysis proteins was different by the groups but the coat proteins were almost the same size among phages. The phylogenetic analysis shows that the sub-groups A-I and A-II of E. coli RNA phages were clearly dispersed into two clusters.
Nishihara
Tohru
T
Department of Biochemistry, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan. tnishihara@ma.0038.net
Fujisaki
Shingo
S
Nishimura
Yukinobu
Y
Minami
Yoshiko
Y
Yubisui
Toshitsugu
T
D017908:Allolevivirus / Q000235:genetics; D000595:Amino Acid Sequence; D001433:Bacteriolysis / Q000235:genetics*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D003090:Coliphages / Q000235:genetics*; D004926:Escherichia coli / Q000821:virology*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D017105:Pseudomonas Phages / Q000235:genetics; D011550:Pseudomonas aeruginosa; D012317:RNA Phages / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D036022:Capsid Proteins