Quantification of alpha1-adrenoceptor subtypes by real-time RT-PCR and correlation with age and prostate volume in benign prostatic hyperplasia patients.
BACKGROUND
We used a real-time reverse transcriptase polymerase chain reaction (RT-PCR) method for quantification of each alpha(1)-adrenoceptor (alpha(1)-AR) subtype expression level, and examined whether age and prostate volume influence human prostate alpha(1)-AR subtype expression.


METHODS
Enrolled in our study were 75 men with lower urinary tract symptoms (LUTS) secondary to untreated benign prostatic hyperplasia (BPH). Real-time RT-PCR was performed using prostate biopsy specimens to quantify the expression level of each alpha(1)-AR subtype.


RESULTS
The median expression level (interquartile range) was 1.24 (0.66-2.32), 0.16 (0.10-0.33), and 1.11 (0.75-2.27) x 1,000 copies/beta-actin for alpha(1a)-, alpha(1b)-, and alpha(1d)-AR mRNA, respectively. The expression levels differed with the individual. The expression levels of alpha(1a)-AR, alpha(1d)-AR, and total alpha(1)-AR mRNA showed a significant positive correlation with patient age, but did not correlate with prostate volume.


CONCLUSIONS
The difference in the expression of the alpha(1)-AR subtype with the patient may be the cause of the difference in the effectiveness of several subtype-selective alpha(1)-AR antagonists from patient to patient. The increase of alpha(1)-AR mRNA expression level with age could be an important factor in the pathogenesis of clinically significant BPH.
Kojima
Yoshiyuki
Y
Department of Nephro-Urology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. ykojima@med.nagoya-cu.ac.jp
Sasaki
Shoichi
S
Shinoura
Hitomi
H
Hayashi
Yutaro
Y
Tsujimoto
Gozoh
G
Kohri
Kenjiro
K
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology / Q000627:therapeutic use; D000367:Age Factors; D000368:Aged; D006801:Humans; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000033:anatomy & histology*; D011470:Prostatic Hyperplasia / Q000235:genetics* / Q000473:pathology*; D018340:Receptors, Adrenergic, alpha-1 / Q000032:analysis* / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000317:Adrenergic alpha-Antagonists; D018340:Receptors, Adrenergic, alpha-1