Methacryloylamidoglutamic acid having porous magnetic beads as a stationary phase in metal chelate affinity chromatography.
We have prepared a novel magnetic metal-chelate adsorbent utilizing methacryloylamidoglutamic acid (MAGA) as a metal-chelating ligand. MAGA was synthesized by using methacryloyl chloride and L-glutamic acid dihydrochloride. Magnetic beads with an average diameter of 50-100 microm were produced by suspension polymerization of 2-hydroxyethyl methacrylate (HEMA) and MAGA in the presence of Fe3O4 particles carried out in an aqueous dispersion medium. Magnetic beads were charged with the Cu2+ ions directly via MAGA for the adsorption of cytochrome c (cyt c) from aqueous solutions. The maximum cyt c adsorption capacity of the Cu2+-chelated beads (0.86 mmol/g Cu2+ loading) was found to be 37 mg/g at pH 8.0 in phosphate buffer. Cyt c adsorption on the poly(HEMA-MAGA) beads was 15.4 mg/g. Cu2+ charging increased the cyt c adsorption significantly (37 mg/g). Cyt c adsorption decreased with increasing temperature. Cyt c molecules could be adsorbed and desorbed five times with these adsorbents without noticeable loss in their cyt c adsorption capacity. The resulting magnetic chelator beads posses excellent long term storage stability.
Altintaş
Evrim Banu
EB
Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey.
Yavuz
Handan
H
Say
Ridvan
R
Denizli
Adil
A
D000818:Animals; D002413:Cations, Divalent / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D045304:Cytochromes c / Q000302:isolation & purification; D005971:Glutamates / Q000737:chemistry*; D006736:Horses; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D016062:Porosity; D013696:Temperature
D002413:Cations, Divalent; D002614:Chelating Agents; D005971:Glutamates; D008689:Methacrylates; C514131:methacryloylamidoglutamic acid; D003300:Copper; D045304:Cytochromes c