title: Immunomodulatory effects of in vitro exposure to organochlorines on T-cell proliferation in marine mammals and mice.
abstract: Marine mammals bioaccumulate various environmental contaminants such as organochlorines (OCs), which biomagnify via the food web. While the immunomodulatory effects of individual OCs have been studied, the effects of mixtures are not well understood. The immunomodulatory effects of polychlorinated biphenyl (PCB) 138, 153, 169, and 180 as well as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and all possible mixtures were examined in marine mammals and mice. Lymphocyte proliferation was significantly modulated by OCs in all species tested, mostly by non-coplanar PCBs, as shown using regression analyses. Correlation analyses showed significant correlations (interpreted as additive effects) between OCs in mice, killer whales, and Steller sea lions. Nonadditive synergistic and antagonistic interactions between OCs were detected in most of the species tested. Toxic equivalency (TEQ) values used for OC toxicity assessment failed to predict the immunomodulatory effects measured in mice and marine mammals. The commonly used mouse model failed to predict immunomodulatory effects in other species. Clustering data suggested that phylogeny does not predict toxicity of OCs. Overall, our data suggest the presence of species-specific sensitivities to different mixtures, in which OCs interactions may be complex and that may exert their effects through dioxinlike or dioxin-independent pathways. Lastly, lymphocyte proliferation, an important part of adaptive immunity, was significantly modulated in mice and marine mammals, suggesting the possibility of increased susceptibility to diseases. These findings will be useful to better characterize the risk associated with OC exposure and possibly lead to new conservation and management strategies.
authors.affiliation: Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, Connecticut 06269, USA. chiharu.mori@uconn.edu
authors.forename: Chiharu
authors.initials: C
authors.lastname: Mori
authors.forename: Brenda
authors.initials: B
authors.lastname: Morsey
authors.forename: Milton
authors.initials: M
authors.lastname: Levin
authors.forename: Prashant R
authors.initials: PR
authors.lastname: Nambiar
authors.forename: Sylvain
authors.initials: S
authors.lastname: De Guise
mesh_terms: D000818:Animals; D010872:Caniformia; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002591:Cetacea; D003208:Concanavalin A; D004347:Drug Interactions; D005260:Female; D007155:Immunologic Factors / Q000633:toxicity*; D051379:Mice; D008815:Mice, Inbred Strains; D010041:Otters; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D013045:Species Specificity; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
chemical_list: D007155:Immunologic Factors; D000072317:Polychlorinated Dibenzodioxins; D003208:Concanavalin A; D011078:Polychlorinated Biphenyls