[Effect of phenolic alkaloids of Menispermum dauricum on thrombosis and platelet aggregation].
OBJECTIVE
To observe the effect of phenolic alkaloids of Menispermum dauricum (PAMD) on thrombosis and platelet aggregation, and to explore its mechanism of action.


METHODS
Thrombosis was observed with arteriovenous shunt thrombus model in rat; platelet aggregation was determined by Born's method; ultrastructure of platelet was observed by transmission electron microscope; TXB2 or 6-keto-PGF1alpha levels were assessed by radioimmunoassay; and NO was determined by colorimetric method.


RESULTS
PAMD dose-dependently inhibited experimental thrombus formation, platelet aggregation induced by ADP, AA and THR in vivo and ultrastructure changes stimulated by THR; PAMD increased the generation of 6-keto-PGF1alpha in thoracic aortae and NO level in plasma; and had no influence on TXB2 release (P > 0.05).


CONCLUSIONS
PAMD inhibited thrombosis and platelet aggregation, and its mechanism might be due to the increase of PGI2 and NO level.
Kong
Xiang-Ying
XY
Department of Pharmacology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. kong0051@hotmail.com
Gong
Pei-Li
PL
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000470:Alkaloids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D044182:Benzylisoquinolines / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D001792:Blood Platelets / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D011464:Epoprostenol / Q000378:metabolism; D008297:Male; D031603:Menispermum / Q000737:chemistry; D009569:Nitric Oxide / Q000097:blood; D010946:Plants, Medicinal / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry; D044005:Tetrahydroisoquinolines / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D013927:Thrombosis / Q000378:metabolism*; D013929:Thromboxane B2 / Q000378:metabolism
D000470:Alkaloids; D044182:Benzylisoquinolines; D044005:Tetrahydroisoquinolines; D009569:Nitric Oxide; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C023968:daurisoline; C035934:dauricine; D011464:Epoprostenol