title: Secretion of human epidermal growth factor by Corynebacterium glutamicum.
abstract: OBJECTIVE
  To examine the secretion of human epidermal growth factor (hEGF) by Corynebacterium glutamicum.
  
  
  RESULTS
  We recently showed that a novel protein-secretion system in C. glutamicum could produce Streptomyces mobaraensis transglutaminase. In the present study, the industrially important protein hEGF was secreted into the culture medium in a fully active form by C. glutamicum and accumulated at a rate of up to 156 mg l(-1) day(-1).
  
  
  CONCLUSIONS
  These results demonstrated that the hEGF protein could be secreted in an active form by C. glutamicum.
  
  
  CONCLUSIONS
  Our data confirmed that the pharmaceutically important human protein hEGF could be efficiently secreted in an active form by the C. glutamicum protein-expression system. Moreover, we demonstrated that this bacterium has potential as a host for the industrial-scale production of human proteins.
authors.affiliation: Institute of Life Sciences, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Date
authors.forename: H
authors.initials: H
authors.lastname: Itaya
authors.forename: H
authors.initials: H
authors.lastname: Matsui
authors.forename: Y
authors.initials: Y
authors.lastname: Kikuchi
mesh_terms: D015153:Blotting, Western / Q000379:methods; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D010957:Plasmids / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004815:Epidermal Growth Factor