[Research on Culex tritaeniorhynchus and Culex pipiens quinquefasciatus intrathoracically infected with attenuated Japanese encephalitis virus SA14-14-2 vaccine strain].
BACKGROUND
To determine if the attenuated Japanese encephalitis (JE) virus SA14-14-2 vaccine strain interacts efficiently with Culex tritaeniorhynchus and Culex pipiens quinquefasciatus, and further to acquire a new knowledge of its characteristics and safety for human beings.


METHODS
Laboratory colonies of the two species of mosquitoes were set up and were inoculated intrathoracically with the attenuated vaccine virus and wild JE virus (Nak), both of which were used with different dilution from 10(-1) to 10(-9). Subsequently, the virus titers in the mosquitoes were detected by the plaque assay.


RESULTS
Inoculated with the vaccine strain, two species of mosquitoes were infected with the titers ranged from 10(0)-10(-3), and the maximum titers in Culex tritaeniorhynchus and Culex pipiens quinquefasciatus were 4.48 logPFU/ml and 5.63 logPFU/ml, respectively. Inoculated with wild JE virus, Culex pipiens quinquefasciatus was infected with titers ranged from 10(0)-10(-5), and the maximum titer in the mosquitoes was 6.59; Culex tritaeniorhynchus was infected with titers ranged from 10(0)-10(-4) and the maximum titer was 5.74 logPFU/ml.


CONCLUSIONS
By intrathoracic infection, the attenuated JE virus SA14-14-2 vaccine strain can replicate in both species of mosquitoes.
Zhang
Yu-zheng
YZ
Yunnan Institute of Endemic Diseases Control and Prevention, Dali 671000, China.
Zhang
Hai-lin
HL
Yu
Yong-xin
YX
Feng
Yun
Y
Dong
Guan-mu
GM
Yang
Wei-hong
WH
Jia
Li-li
LL
Yao
Ya-fu
YF
D000818:Animals; D003465:Culex / Q000145:classification / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D004672:Encephalitis, Japanese / Q000821:virology; D006801:Humans; D007303:Insect Vectors / Q000821:virology*; D022321:Japanese Encephalitis Vaccines / Q000276:immunology; D013045:Species Specificity; D014613:Vaccines, Attenuated / Q000276:immunology; D010948:Viral Plaque Assay
D022321:Japanese Encephalitis Vaccines; D014613:Vaccines, Attenuated