title: Differential expression pattern of coq-8 gene during development in Caenorhabditis elegans.
abstract: Coenzyme Q (Q) and the genes involved in its biosynthesis are involved in aging and development of Caenorhabditis elegans. Q is synthesized by at least eight highly conserved nuclear coq genes, but this biosynthesis pathway and its regulation is not known. The coq-8 gene sequence has homology to the ABC-1 family kinases and is the only known candidate for a possible regulation of this pathway. To study coq-8 expression pattern, we have developed a C. elegans transgenic strain expressing ubiquinone biosynthesis coq-8 gene promoter and GFP construct. We show here an age-dependent specific pattern from embryo to senescence for COQ-8 protein expression. Expression in embryo was triggered by a defined group of blastomers before morphogenesis. In elderly nematodes expression was only observed in nervous system, whilst expression in larvae was also detected in hypodermis, muscles and coelomocytes. Global expression provide a regulated pattern during life cycle of the nematode.
authors.affiliation: Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide, 41013 Sevilla, Spain.
authors.forename: Claudio
authors.initials: C
authors.lastname: Asencio
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Rodríguez-Aguilera
authors.forename: Rafael
authors.initials: R
authors.lastname: Vázquez
authors.forename: Howard
authors.initials: H
authors.lastname: Baylis
authors.forename: Juan
authors.initials: J
authors.lastname: Cabello
authors.forename: Ralf
authors.initials: R
authors.lastname: Schnabel
authors.forename: Angela
authors.initials: A
authors.lastname: Gavilán
authors.forename: Plácido
authors.initials: P
authors.lastname: Navas
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology*; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007814:Larva; D014018:Tissue Distribution; D014451:Ubiquinone / Q000235:genetics*
chemical_list: D014451:Ubiquinone; D049452:Green Fluorescent Proteins; C030778:ubiquinone 8