title: On-line identification of the constituents of Buyang Huanwu decoction in pig serum using combined HPLC-DAD-MS techniques.
abstract: Buyang Huanwu decoction (BYHWD) is a widely used Chinese traditional compound medicine that has proved effective in treating cerebrovascular illnesses; however, its active substances have remained unknown. In this paper, serum chemistry and combined high-performance liquid chromatography (HPLC), photodiode-array detection and mass-spectrometry techniques were used to study the constituents of BYHWD from pig serum after oral administration. A total of 45 characteristic HPLC peaks were detected from serum containing drug. The chemical structures of nine of the peaks were tentatively elucidated as 7,3'-dihydroxy-4'-methoxyisoflavone-7-O-glucuronide (P1), 7-hydroxy-4'-methoxyisoflavone-7-O-glucuronide (P2), 7,2',4'-trihydroxy-3'-methoxyisoflavane-7-O-sulphate (P3), 3-hydroxy-9,10-dimethoxypterocarpan-3-O-glucuronide (P4), 7,2'-dihydroxy-3',4'-dimethoxyisoflavane-7-O-glucuronide (P5), 3-hydroxy-9,10-dimethoxypterocarpane-3-O-sulphate (P6), 4(1H)-quinolinone (P7 or P8), 4-hydroxyquinoline (P8 or P7) and oleic acid (P9). All of the identified peaks, with the exception of P9, were metabolites of the constituents of BYHWD in vivo.
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, PR China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Yang
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Cai
authors.forename: Hongyu
authors.initials: H
authors.lastname: Liu
authors.forename: Xinxin
authors.initials: X
authors.lastname: Guo
authors.forename: Changling
authors.initials: C
authors.lastname: Li
authors.forename: Mingying
authors.initials: M
authors.lastname: Shang
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Yuying
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D047309:Flavones / Q000032:analysis; D008297:Male; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine; D013556:Swine, Miniature / Q000097:blood*
chemical_list: D004365:Drugs, Chinese Herbal; D047309:Flavones; C460102:buyang huanwu