title: Role of LrpC from Bacillus subtilis in DNA transactions during DNA repair and recombination.
abstract: Bacillus subtilis LrpC is a sequence-independent DNA-binding and DNA-bending protein, which binds both single-stranded (ss) and double-stranded (ds) DNA and facilitates the formation of higher order protein-DNA complexes in vitro. LrpC binds at different sites within the same DNA molecule promoting intramolecular ligation. When bound to separate molecules, it promotes intermolecular ligation, and joint molecule formation between a circular ssDNA and a homologous ssDNA-tailed linear dsDNA. LrpC binding showed a higher affinity for 4-way (Holliday) junctions in their open conformation, when compared with curved dsDNA. Consistent with these biochemical activities, an lrpC null mutant strain rendered cells sensitive to DNA damaging agents such as methyl methanesulfonate and 4-nitroquinoline-1-oxide, and showed a segregation defect. These findings collectively suggest that LrpC may be involved in DNA transactions during DNA repair and recombination.
authors.affiliation: Department of Microbial Biotechnology, Centro Nacional Biotecnología, CSIC Darwin 3, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: López-Torrejón
authors.forename: María I
authors.initials: MI
authors.lastname: Martínez-Jiménez
authors.forename: Silvia
authors.initials: S
authors.lastname: Ayora
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001665:Binding Sites; D002467:Cell Nucleus / Q000648:ultrastructure; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D045566:DNA, Cruciform / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D011995:Recombination, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D045566:DNA, Cruciform; D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins