Identification of single eukaryotic cells with micro-Raman spectroscopy.
For a fast identification of eukaryotic cells such as yeast species without a cultivation step it should be possible to perform the investigation on only one single cell. Since yeasts as eukaryotes are heterogeneous and their Raman spectra are therefore dependent on the measuring position, one Raman spectra is not representative of the whole cell. In this contribution we demonstrate the application of average Raman spectra of a line scan over single yeast cells. These average spectra are used for classification with the help of a support vector machine.
Rösch
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Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, 07743 Jena, Germany.
Harz
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Peschke
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Ronneberger
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O
Burkhardt
H
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Popp
J
J
D002175:Candida / Q000737:chemistry / Q000145:classification / Q000166:cytology; D005057:Eukaryotic Cells / Q000737:chemistry / Q000145:classification* / Q000166:cytology; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000145:classification / Q000166:cytology; D013059:Spectrum Analysis, Raman / Q000379:methods*; D015003:Yeasts / Q000737:chemistry / Q000145:classification / Q000166:cytology