Lupane-type saponins from leaves of Acanthopanax sessiliflorus and their inhibitory activity on pancreatic lipase.
Three known saponins, chiisanoside, 11-deoxyisochiisanoside, and isochiisanoside, and one novel saponin, 3,4-seco-4(23),20(29)-lupadiene-3,28-dioic acid 28-O-alpha-l-rhamnopyranosyl (1-->4)-beta-d-glucopyranosyl (1-->6)-beta-d-glucopyranoside, referred to as sessiloside, were isolated from a hot water extract of Acanthopanax sessiliflorus leaves. All of these saponins were lupane-type triterpene triglycosides, and their concentrations were 4.1, 1.0, 0.5, and 0.4% (w/w) of the total extract, respectively. Sessiloside and chiisanoside inhibited pancreatic lipase activity in vitro, and addition of the saponin-rich fraction to a high-fat diet suppressed the body weight gain of mice. The possibility of application of the lupane-type saponins from A. sessiliflorus leaves to the treatment of obesity is discussed.
Yoshizumi
Kazuma
K
Central Research Laboratory, Fancl Corporation, 12-13 Kamishinano, Totsuka-ku, Yokohama, Kanagawa 244-0806, Japan.
Hirano
Kaoru
K
Ando
Hidehiro
H
Hirai
Yasuaki
Y
Ida
Yoshiteru
Y
Tsuji
Tomoko
T
Tanaka
Tamotsu
T
Satouchi
Kiyoshi
K
Terao
Junji
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004041:Dietary Fats / Q000008:administration & dosage; D028001:Eleutherococcus / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006461:Hemolysis; D006358:Hot Temperature; D008049:Lipase / Q000037:antagonists & inhibitors*; D051379:Mice; D008813:Mice, Inbred ICR; D010179:Pancreas / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D014867:Water; D015430:Weight Gain / Q000187:drug effects; D053978:Pentacyclic Triterpenes
D004041:Dietary Fats; D004791:Enzyme Inhibitors; D008049:Lipase; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; D014867:Water; C480546:lupane; D053978:Pentacyclic Triterpenes