Preoperative oral immune-enhancing nutritional supplementation corrects TH1/TH2 imbalance in patients undergoing elective surgery for colorectal cancer.
OBJECTIVE
Recent studies have shown that the type 1/2 CD4+ T cell (Th1/Th2) balance shifts toward Th2 dominance in cancer-bearing state or by surgical stress. Perioperative immunonutrition is reported to improve the outcome in patients with gastrointestinal cancer. This study was designed to investigate whether preoperative immunonutrition corrects the impaired Th1/Th2 balance in the perioperative period.


METHODS
Thirty-six patients with colorectal cancer were prospectively divided into two groups as follows: preoperative oral intake supplementation with a formula enriched with arginine, omega-3 fatty acids and ribonucleic acid for five days (supplemented group; n = 19); and (control group; n = 17). Blood sampling was performed before supplementation, on the morning of surgery, and 3, 7, and 14 days postoperatively. The proportions of CD4+ T cells producing intracellular cytokines (interferon-gamma and interleukin-4) were measured by flow cytometry.


RESULTS
In the preoperative period, the proportions of CD4+ T cells producing interleukin-4 significantly decreased and Th1/Th2 ratio significantly increased on the morning of surgery compared with those before supplementation. In the postoperative period, the proportions of CD4+ T cells producing interferon-gamma in both groups maintained the preoperative level. The proportions of CD4+ T cells producing interleukin-4 in the control group showed a gradual increase from the preoperative level, which implies Th2 dominant shift. In contrast, the supplemented group maintained the preoperative level of Th1/Th2 ratio.


CONCLUSIONS
Preoperative immunonutrition corrects impaired Th1/Th2 balance in both cancer-bearing state and the postoperative period. This correction may be one of the important determinants of the clinical benefits of immunonutrition.
Matsuda
Akihisa
A
Surgery for Organ Function and Biologic Regulation (Department of Surgery 1), Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo, Japan. a-matsu@nms.ac.jp
Furukawa
Kiyonori
K
Takasaki
Hideaki
H
Suzuki
Hideyuki
H
Kan
Hayato
H
Tsuruta
Hiroyuki
H
Shinji
Seiichi
S
Tajiri
Takashi
T
D000284:Administration, Oral; D000368:Aged; D015179:Colorectal Neoplasms / Q000097:blood / Q000276:immunology* / Q000601:surgery*; D016207:Cytokines / Q000097:blood; D019587:Dietary Supplements; D017558:Elective Surgical Procedures; D005260:Female; D006801:Humans; D007167:Immunotherapy; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D011300:Preoperative Care; D018417:Th1 Cells; D018418:Th2 Cells; D016896:Treatment Outcome
D016207:Cytokines