title: Stability of Delta(9)-tetrahydrocannabinol (THC) in oral fluid using the Quantisal collection device.
abstract: This article details the stability of Delta(9)-tetrahydrocannabinol (THC) in oral fluid during collection, extraction and storage. Oral fluid is being increasingly used as the specimen of choice for the detection of drug use in various applications. Studies to determine the extraction efficiency of THC from the collection buffer and stability under various laboratory storage conditions were carried out. THC was extracted from the collection pad and buffer with an average efficiency over 80% and was stable in Quantisal oral fluid extraction buffer when stored at refrigerated temperatures. Fluorescent lighting caused THC losses of over 50%, however the presence of the pad reduced the loss. In the dark, the loss of THC at room temperature was approximately 20% over 14 days. When stored with the serum separators in place, THC losses were significant. After 3 days, THC concentration was reduced by almost 30%, and after 14 days, 60% of the drug was lost and the losses were not concentration dependent.
authors.affiliation: Immunalysis Corporation, Pomona, CA 91767, USA. cmoore@immunalysis.com
authors.forename: C
authors.initials: C
authors.lastname: Moore
authors.forename: M
authors.initials: M
authors.lastname: Vincent
authors.forename: S
authors.initials: S
authors.lastname: Rana
authors.forename: C
authors.initials: C
authors.lastname: Coulter
authors.forename: A
authors.initials: A
authors.lastname: Agrawal
authors.forename: J
authors.initials: J
authors.lastname: Soares
mesh_terms: D002021:Buffers; D003624:Darkness; D013759:Dronabinol / Q000032:analysis / Q000493:pharmacokinetics*; D004355:Drug Stability; D005453:Fluorescence; D053593:Forensic Toxicology; D006213:Hallucinogens / Q000032:analysis / Q000493:pharmacokinetics*; D006801:Humans; D012463:Saliva / Q000737:chemistry*; D013048:Specimen Handling / Q000295:instrumentation*; D015813:Substance Abuse Detection; D013696:Temperature
chemical_list: D002021:Buffers; D006213:Hallucinogens; D013759:Dronabinol