Growth characteristics of Bacillus anthracis compared to other Bacillus spp. on the selective nutrient media Anthrax Blood Agar and Cereus Ident Agar.
Anthrax Blood Agar (ABA) and Cereus Ident Agar (CEI) were evaluated as selective growth media for the isolation of Bacillus anthracis using 92 B. anthracis and 132 other Bacillus strains from 30 species. The positive predictive values for the identification of B. anthracis on ABA, CEI, and the combination of both were 72%, 71%, and 90%, respectively. Thus, less than 10% of all species were misidentified using both nutrient media. Species which might be misidentified as B. anthracis were B. cereus, B. mycoides, and B. thuringiensis. Particularly, 30% of B. weihenstephanensis strains were misidentified as B. anthracis.
Tomaso
Herbert
H
Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany. HerbertTomaso@bundeswehr.org
Bartling
Carsten
C
Al Dahouk
Sascha
S
Hagen
Ralf M
RM
Scholz
Holger C
HC
Beyer
Wolfgang
W
Neubauer
Heinrich
H
D000362:Agar; D000881:Anthrax / Q000382:microbiology; D001408:Bacillus anthracis / Q000254:growth & development* / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000379:methods; D003470:Culture Media; D006801:Humans
D003470:Culture Media; D000362:Agar