Detection of human MCP-4/CCL13 isoforms by SELDI immunoaffinity capture.
Monocyte Chemoattractant Proteins 4 (MCP-4/CCL13) is a member of a distinct, structurally-related subclass of CC chemokines mainly involved in recruitment of eosinphils to inflammatory sites. Recent evidence demonstrates that serum level of this protein strongly increases following high dose IL-2 immunotherapy. The physiological form of human MCP-4/CCL13 has yet to be purified. Therefore, the primary structure of the biologically relevant (mature) form has not been established. By using SELDI immunoaffinity capture technology we describe two mature isoforms both present in serum before and after high-dose IL-2 immunotherapy.
Rossi
Leonardo
L
Department of Human Morphology and Applied Biology, University of Pisa, 56126 Pisa, Italy. leoros@biomed.unipi.it
Moharram
Ramy
R
Martin
Brian M
BM
White
Richard L
RL
Panelli
Monica C
MC