Sensitive and specific detection of Xanthomonas axonopodis pv. citri by PCR using pathovar specific primers based on hrpW gene sequences.
A sensitive and specific assay was developed to detect citrus bacterial canker caused by Xanthomonas axonopodis pv. citri, in leaves and fruits of citrus. Primers XACF and XACR from hrpW homologous to pectate lyase, modifying the structure of pectin in plants, were used to amplify a 561 bp DNA fragment. PCR technique was applied to detect the pathogen in naturally or artificially infected leaves of citrus. The PCR product was only produced from X. axonopodis pv. citri among 26 isolates of Xanthomonas strains, Escherichia coli (O157:H7), Pectobacterium carotovorum subsp. carotovorum, and other reference microbes.
Park
Dong Suk
DS
National Institute of Agricultural Biotechnology, Rural Development Administration, 441-707 Suwon, Republic of Korea. dspark@rda.go.kr
Hyun
Jae Wook
JW
Park
Young Jin
YJ
Kim
Jung Sun
JS
Kang
Hee Wan
HW
Hahn
Jang Ho
JH
Go
Seung Joo
SJ
D002957:Citrus; D017931:DNA Primers / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015151:Immunoblotting; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014974:Xanthomonas / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers; D004271:DNA, Fungal