Crystallization and preliminary X-ray crystallographic analysis of PAS factor from Vibrio vulnificus.
Plasmid Achromobacter secretion (PAS) factor is a putative secretion factor that induces the secretion of periplasmic proteins. PAS factor from Vibrio vulnificus was crystallized at 294 K by the hanging drop vapor-diffusion method. It was isolated as a monomer during the purification procedures. The native crystal belongs to the F222 space group with unit cell parameters a=56.1, b=74.4, c=80.0 A, a=b=g=90 degrees. The crystal was soaked in cryoprotectant containing 1 M NaBr for 1 h for MAD phasing. The diffraction limit of the Br-MAD data set was 1.9 A using synchrotron X-ray irradiation at beam line BL-18B at the Photon Factory, Japan.
Lee
Jun Hyuck
JH
Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
Kim
Soo Young
SY
Rho
Seong-Hwan
SH
Im
Young Jun
YJ
Kim
Young Ran
YR
Kim
Mun-Kyoung
MK
Kang
Gil Bu
GB
Rhee
Joon Haeng
JH
Eom
Soo Hyun
SH
D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D041261:Vibrio vulnificus / Q000737:chemistry*
D001426:Bacterial Proteins