Effect of photodynamic therapy on tumor angiogenesis and metastasis in mice bearing Lewis lung carcinoma.
OBJECTIVE
To investigate the influence of photodynamic therapy (PDT) on the tumor angiogenesis and metastasis.


METHODS
The effect of PDT with a photosensitizer 5-aminolaevulinic acid (ALA) on tumor metastasis and angiogenesis was studied on the model of subcutaneously implanted Lewis lung carcinoma (3LL) in C57Bl/6 mice. VEGF level in blood serum of animals was evaluated by ELISA.


RESULTS
It has been shown that application of ALA-PDT resulted in the lowered rate of metastatic spreading and decreased VEGF level in blood serum of 3LL-bearing mice, and morphologic alterations of vascular system in tumor tissue.


CONCLUSIONS
The antitumor action of PDT using ALA as a photosensitizer is caused partly by antimetastatic and photodamaging effect mediated by vascularization disturbances in tumor tissue.
Lisnjak
I O
IO
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine.
Kutsenok
V V
VV
Polyschuk
L Z
LZ
Gorobets
O B
OB
Gamaleia
N F
NF
D000622:Aminolevulinic Acid / Q000627:therapeutic use; D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000098:blood supply / Q000628:therapy*; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D017319:Photosensitizing Agents / Q000627:therapeutic use; D010789:Phototherapy; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
D017319:Photosensitizing Agents; D042461:Vascular Endothelial Growth Factor A; D000622:Aminolevulinic Acid