Transcriptional slippage in mxiE controls transcription and translation of the downstream mxiD gene, which encodes a component of the Shigella flexneri type III secretion apparatus.
The Shigella flexneri transcription activator MxiE is produced by transcriptional slippage from two overlapping open reading frames. By using plasmids encoding a mxiD-lacZ translational fusion, we showed that transcriptional slippage in mxiE influences both transcription and translation of the downstream mxiD gene encoding an essential component of the type III secretion apparatus.
Penno
Christophe
C
Unité de Pathogénie Microbienne Moléculaire, INSERM U389, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France.
Parsot
Claude
C
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D018965:Frameshifting, Ribosomal; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D014176:Protein Biosynthesis; D012763:Shigella flexneri / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014158:Transcription, Genetic
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C079723:MxiD protein, Shigella; C079724:MxiE protein, Shigella flexneri; D014157:Transcription Factors