Determination of acrylamide and acrylic acid by isocratic liquid chromatography with pulsed electrochemical detection.
The electrochemical behaviour of the polycrystalline platinum electrode towards the oxidation/reduction of short-chain unsaturated aliphatic molecules such as acrylamide and acrylic acid was investigated in acidic solutions. Analytes were separated by reverse phase liquid chromatographic and quantified using a pulsed amperometric detection. A new two-step waveform, is introduced for detection of acrylamide and acrylic acid. Detection limits (LOD) of 20 nM (1. 4 microg/kg) and 45 nM (3.2 microg/kg) were determined in water solutions containing acrylamide and acrylic acid, respectively. Compared to the classical three-step waveform, the proposed two-step waveform shows favourable analytical performance in terms of LOD, linear range, precision and improved long-term reproducibility. The proposed analytical method combined with clean-up procedure accomplished by Carrez clearing reagent and subsequent extraction with a strong cation exchanger cartridges (SPE), was successfully used for the quantification of low concentrations of acrylamide in foodstuffs such as coffee and potato fries.
Casella
Innocenzo G
IG
Dipartimento di Chimica dell' Universita', Via N. Sauro 85, 85100 Potenza, Italy. casella@unibas.it
Pierri
Marianna
M
Contursi
Michela
M
D020106:Acrylamide / Q000032:analysis*; D000179:Acrylates / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D012680:Sensitivity and Specificity
D000179:Acrylates; D020106:Acrylamide; C036658:acrylic acid