Succinate semialdehyde dehydrogenase deficiency does not down-regulate gamma-hydroxybutyric acid binding sites in the mouse brain.
We investigated whether succinate semialdehyde dehydrogenase deficiency alters gamma-hydroxybutyric acid (GHB) receptor characteristics due to elevation of GHB levels in the mouse brain. The membrane homogenate binding and quantitative autoradiography using [3H]NCS-382 revealed no significant changes in the affinity (Kd), receptor density (Bmax), or displacement potency (IC50) in various brain regions of Aldh5a1-/- vs. Aldh5a1+/+ mice.
Mehta
Ashok K
AK
Department of Pharmacology, MC 7764, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA.
Gould
Georgianna G
GG
Gupta
Maneesh
M
Carter
Lawrence P
LP
Gibson
K Michael
KM
Ticku
Maharaj K
MK
D000818:Animals; D001567:Benzocycloheptenes / Q000378:metabolism; D001921:Brain / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism; D015536:Down-Regulation; D006624:Hippocampus / Q000378:metabolism; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism*; D050644:Succinate-Semialdehyde Dehydrogenase / Q000172:deficiency*
C039403:4-hydroxybutyric acid receptor; D001567:Benzocycloheptenes; D011956:Receptors, Cell Surface; C066471:NCS 382; D050644:Succinate-Semialdehyde Dehydrogenase