Chemo-enzymatic synthesis of allyl penta-N-acetyl-chitopentaose.
Cell density cultivation of recombinant Escherichia coli strains harboring the nodC gene (encoding chitooligosaccharide synthase) from Azorhizobium caulinodans has been previously described as a practical method for the preparation of gram-scale quantities of penta-N-acetyl-chitopentaose. We have now extended this method to the production of allylated derivative of penta-N-acetyl-chitopentaose by using allyl 2-acetamido-2-deoxy-beta-d-glucopyranoside (2) as the initial acceptor for the synthesis of target pentaoside in vivo.
Huang
Gang-Liang
GL
School of Life Science, Shandong University, Jinan City 250100, China. hgl226@126.com
Zhang
Da-Wei
DW
Zhao
Hong-Juan
HJ
Zhang
Hou-Cheng
HC
Wang
Peng-George
PG
D002240:Carbohydrate Sequence; D002686:Chitin / Q000031:analogs & derivatives / Q000138:chemical synthesis*; D004926:Escherichia coli / Q000235:genetics; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data
D002686:Chitin; D008025:Ligases