title: Gene expression profiling of early follicular development in primordial, primary, and secondary follicles.
abstract: OBJECTIVE
  To study global gene expression profiles of early folliculogenesis in primordial, primary, and secondary follicles.
  
  
  METHODS
  A cDNA microarray study using amplified RNAs from isolated follicles.
  
  
  METHODS
  Experimental animal study.
  
  
  METHODS
  Female ICR strain mice (12 days old).
  
  
  METHODS
  Isolation of follicles at each stage, RNA isolation and amplification, microarray hybridization, and statistical analysis for microarray.
  
  
  METHODS
  Gene lists of various functional groups with an estimated false discovery rate of 5%. Among them, platelet-derived growth factors (PDGFs) and receptors were localized by immunohistochemistry in mouse ovaries.
  
  
  RESULTS
  We analyzed a list of genes according to function, such as apoptosis, cell cycle, cell proliferation and maintenance, cytoskeleton, extracellular matrix, and signal transduction, as well as according to frequency. Among the list of genes, we found all PDGFs (A, B, C, and D) and receptors (alpha and beta) are expressed with differential expression patterns in the oocytes and ovarian cells according to stage of follicular development.
  
  
  CONCLUSIONS
  The present report suggests that genome-wide expression profiling using microarray after RNA amplification may become a useful tool to better understand the molecular mechanism(s) involved in early ovarian folliculogenesis.
authors.affiliation: CHA Research Institute, Infertility Medical Center, CHA General Hospital, Seoul, Korea.
authors.forename: Se-Jin
authors.initials: SJ
authors.lastname: Yoon
authors.forename: Kyeoung-Hwa
authors.initials: KH
authors.lastname: Kim
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Chung
authors.forename: Dong-Hee
authors.initials: DH
authors.lastname: Choi
authors.forename: Woo-Sik
authors.initials: WS
authors.lastname: Lee
authors.forename: Kwang-Yul
authors.initials: KY
authors.lastname: Cha
authors.forename: Kyung-Ah
authors.initials: KA
authors.lastname: Lee
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D051379:Mice; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development* / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics
chemical_list: D010982:Platelet-Derived Growth Factor; D017479:Receptors, Platelet-Derived Growth Factor