title: Determining the 3D structure of human ASC2 protein involved in apoptosis and inflammation.
abstract: ASC2 structure has been well defined by 1141 NOE experimental restraints. The model consists of five alpha helices. alpha-Helices are connected by short random structure loops. The sole exception is the loop connecting helices 2 and 3, which has a 20-residue length. Folding generally agrees with the folding of recently published death domain structures in which alpha-helix structures have been reported. In spite of structural similarity, amino acid sequence homology with the most similar protein (ASC1) is just 64%. DD, DED, and CASP protein structures present six helices along their sequences; ASC2 presents 5 well-defined helices due to long distance restraints. However, a helical fragment was observed between amino acids 38 and 42 (representing helix 3) in the death domains when constructing the model.
authors.affiliation: Fundación Instituto de Inmunologia de Colombia (FIDIC). Cra 50 N. 26-00 Bogotá, Colombia. fabiola_espejo@fidic.org
authors.forename: Fabiola
authors.initials: F
authors.lastname: Espejo
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Patarroyo
mesh_terms: D027182:Amino Acid Transport System y+ / Q000737:chemistry; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry*; D002942:Circular Dichroism; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007249:Inflammation; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010957:Plasmids / Q000378:metabolism; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D012261:Ribonucleoproteins / Q000737:chemistry*; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D027182:Amino Acid Transport System y+; D051017:Apoptosis Regulatory Proteins; C490350:POP1 protein, human; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; C498799:SLC7A10 protein, human