[Molecular identification of species within the Mycobacterium tuberculosis complex using regions of difference].
The differentiation within the Mycobacterium tuberculosis complex (MTBC) based on phenotypic methods is long and does not give an unambiguous result in every case whereas the advance in genetic knowledge leads to new views. Thus, regions of difference (RD), that seem to characterize the species of the MTBC, have been identified. Amplification methods, targeted on these zones, have been developed then. The study of four regions (RD1, RD5, RD9, RD10) has been done on 64 isolates formerly identified thanks to phenotypic methods. Genotypic results confirm phenotypic identifications except in one case. This strain initially identified as M. tuberculosis and isolated from a Gabonese patient, corresponds, according to genotypic identification, to M. africanum. Since phenotypic characterization of M. africanum is difficult, this method would allow to accurately determine the true prevalence of this specie. Moreover, the study of RD10 doesn't seem to be informative. The amplification of only two RD, RD1 and RD9 carries out the identification of all M. tuberculosis and M. bovis BCG isolates. M. bovis and M. africanum will be then identified thanks to RD5. Thus, this easy and rapid method of identification of the major species of MTBC seems to be appropriated for a routine use.
Gaudrat
F
F
Laboratoire de bactériologie, Hôpital Bretonneau, CHRU de Tours.
Sauget
A S
AS
Cottencin
I
I
Giraudet
L
L
Morange
V
V
Mereghetti
L
L
Goudeau
A
A
Lanotte
P
P
D000995:Antitubercular Agents / Q000627:therapeutic use*; D001483:Base Sequence; D017931:DNA Primers; D005784:Gene Amplification; D005838:Genotype; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000187:drug effects / Q000235:genetics*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy*
D000995:Antitubercular Agents; D017931:DNA Primers