title: The Vibrio harveyi GTPase CgtAV is essential and is associated with the 50S ribosomal subunit.
abstract: It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtAV is not essential. Here we show that cgtAV was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtAV did not result in cell elongation. CgtAV is associated with the large ribosomal particle. In light of our results, we predict that the V. harveyi CgtAV protein plays a similar essential role to that seen for Obg/CgtA proteins in other bacteria.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, MI 48109-1048, USA.
authors.forename: A E
authors.initials: AE
authors.lastname: Sikora
authors.forename: R
authors.initials: R
authors.lastname: Zielke
authors.forename: K
authors.initials: K
authors.lastname: Datta
authors.forename: J R
authors.initials: JR
authors.lastname: Maddock
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D014733:Vibrio / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D012269:Ribosomal Proteins; C027624:ribosomal protein L11; C108621:CgtA protein, bacteria; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020559:Monomeric GTP-Binding Proteins