Simultaneous separation of acid and basic bioactive peptides by electrodialysis with ultrafiltration membrane.
beta-Lactoglobulin (beta-lg), one of the major whey components, can release by enzymatic hydrolysis different bioactive peptidic sequences according to the enzyme used. However, these protein hydrolysates have to be fractionated to obtain peptides in a more purified form. The aim of the present work was to evaluate the feasibility of separating peptides from a beta-lg hydrolysate using an ultrafiltration (UF) membrane stacked in an electrodialysis (ED) cell and to study the effect of pH on the migration of basic/cationic and acid/anionic peptides in the ED configuration. Electrodialysis with ultrafiltration membrane (EDUF) appeared to be a selective method of separation since amongst a total of 40 peptides in the raw hydrolysate, only 13 were recovered in the separated adjacent solutions (KCl 1 and KCl 2). Amongst these 13 migrating peptides, 3 acid/anionic peptides migrated only in one compartment (KCl 1), while 3 basic/cationic peptides migrated only in the second compartment (KCl 2) and that whatever the pH conditions of the hydrolysate solution. Furthermore, the highest migration was obtained for the ACE-inhibitory peptide beta-lg 142-148, with a value of 10.75%. The integrity of the UF membrane was kept and EDUF would minimize the fouling of UF membrane.
Poulin
Jean-François
JF
Institute of Nutraceuticals and Functional Foods (INAF) and Dairy Research Centre (STELA), Department of Food Sciences and Nutrition, Laval University, Que., Canada G1K 7P4.
Amiot
Jean
J
Bazinet
Laurent
L
D000818:Animals; D000838:Anions; D002412:Cations; D002417:Cattle; D045424:Complex Mixtures / Q000302:isolation & purification; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D006863:Hydrogen-Ion Concentration; D007782:Lactoglobulins / Q000737:chemistry* / Q000302:isolation & purification*; D008567:Membranes, Artificial; D017551:Microdialysis / Q000295:instrumentation* / Q000379:methods; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011492:Protein Hydrolysates / Q000737:chemistry / Q000302:isolation & purification; D014462:Ultrafiltration / Q000295:instrumentation* / Q000379:methods
D000838:Anions; D002412:Cations; D045424:Complex Mixtures; D007782:Lactoglobulins; D008567:Membranes, Artificial; D010455:Peptides; D011492:Protein Hydrolysates