N49 phospholipase A2, a unique subgroup of snake venom group II phospholipase A2.
A novel phospholipase A2 (PLA2) with Asn at its site 49 was purified from the snake venom of Protobothrops mucrosquamatus by using SP-Sephadex C25, Superdex 75, Heparin-Sepharose (FF) and HPLC reverse-phage C18 chromatography and designated as TM-N49. It showed a molecular mass of 13.875 kDa on MALDI-TOF. TM-N49 does not possess enzymatic, hemolytic and hemorrhagic activities. It fails to induce platelet aggregation by itself, and does not inhibit the platelet aggregation induced by ADP. However, it exhibits potent myotoxic activity causing inflammatory cell infiltration, severe myoedema, myonecrosis and myolysis in the gastrocnemius muscles of BALB/c mice. Phylogenetic analysis found that that TM-N49 combined with two phospholipase A2s from Trimeresurus stejnegeri, TsR6 and CTs-R6 cluster into one group. Structural and functional analysis indicated that these phospholipase A2s are distinct from the other subgroups (D49 PLA2, S49 PLA2 and K49 PLA2) and represent a unique subgroup of snake venom group II PLA2, named N49 PLA2 subgroup.
Wei
Ji-Fu
JF
Allergy and Inflammation Research Institute, the Shantou University Medical College, Shantou, Guangdong, 515031, China.
Wei
Xiao-Long
XL
Chen
Qiu-Yu
QY
Huang
Tian
T
Qiao
Li-Ya
LY
Wang
Wan-Yu
WY
Xiong
Yu-Liang
YL
He
Shao-Heng
SH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003435:Crotalid Venoms / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D054501:Group II Phospholipases A2; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000187:drug effects; D010741:Phospholipases A / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D054467:Phospholipases A2; D010802:Phylogeny; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D030162:Reptilian Proteins; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017841:Trimeresurus
D003435:Crotalid Venoms; D030162:Reptilian Proteins; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; C510097:TM-N49 protein, Protobothrops mucrosquamatus