[Molecular biological identification of Batai virus isolated in China].
BACKGROUND
To study the molecular characteristics of YN92-4 strain isolated from mosquitoes in Yunnan Province and define its classification.


METHODS
The S segment of YN92-4 strain was amplified and sequenced by 2 different sets of primers. The phylogenic tree of S fragment was constructed by Phylip bio-software. The amino acid sequences of N and NSs proteins were also studied.


RESULTS
YN92-4 strain could be amplified by 2 sets of primers respectively, S segment showed a highest homology with Batai virus (X73464), reached 96.4%, the homology of protein N and NSs amio-acid sequence with Batai virus was 99.1% and 98% respectively.


CONCLUSIONS
The YN92-4 strain belongs to Batai virus, this is the first report of molecular biological identification of Batai virus in China.
Fu
Shi-hong
SH
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Sun
Xiao-hong
XH
Wang
Huan-yu
HY
Cao
Yu-xi
YX
Wang
Huan-qin
HQ
Liu
Wei-bin
WB
Tao
San-ju
SJ
Liang
Guo-dong
GD
D000595:Amino Acid Sequence; D000818:Animals; D002042:Bunyamwera virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D002681:China; D009033:Culicidae / Q000821:virology*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D012367:RNA, Viral