A single nucleotide polymorphism in CHAT influences response to acetylcholinesterase inhibitors in Alzheimer's disease.
BACKGROUND
Alzheimer's disease (AD) is a devastating neurodegeneration with a characteristic deficit in cholinergic neurotransmission. Treatment with acetylcholinesterase (AChE) inhibitors aims to reverse this deficit and does ameliorate the decline in cognition in some AD patients, although response is variable.


OBJECTIVE
To examine whether sequence variation in the gene encoding choline acetyltransferase (CHAT), which encodes the major catalytic enzyme of the cholinergic pathway, predicts response to AChE inhibitors.


METHODS
Alzheimer's disease patients (121) were treated with cholinesterase inhibitors and the effect of treatment on cognition was measured using the Mini Mental State Examination (MMSE). Six polymorphisms in CHAT were analysed for association with change in MMSE score.


RESULTS
After correction for multiple testing, we found one SNP, rs733722, in a promoter region of CHAT, is associated with response of AD patients to cholinesterase inhibitors (P = 0.03) and accounts for 6% of the variance in response to AChE inhibitors.


CONCLUSIONS
Rs733722 represents a putative marker of response to AChE inhibitors in AD patients.
Harold
Denise
D
Department of Psychological Medicine, Cardiff University, Cardiff, UK.
Macgregor
Stuart
S
Patterson
Cathryn E
CE
Hollingworth
Paul
P
Moore
Pamela
P
Owen
Michael J
MJ
Williams
Julie
J
O'Donovan
Michael
M
Passmore
Peter
P
McIlroy
Stephen
S
Jones
Lesley
L
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology* / Q000235:genetics*; D002384:Catalysis; D002795:Choline O-Acetyltransferase / Q000037:antagonists & inhibitors* / Q000235:genetics*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D010597:Pharmacogenetics / Q000379:methods*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide
D002800:Cholinesterase Inhibitors; D002795:Choline O-Acetyltransferase