Modulatory role of lipoic acid on adriamycin-induced testicular injury.
The present study investigated the protective efficacy of dl-alpha-lipoic acid (LA) on adriamycin (ADR)-induced oxidative damage in rat testis. Adult male albino rats of Wistar strain were administered ADR (1 mg/kg body weight, i.v.), once a week for 10 weeks. ADR injected rats showed increased oxidative stress with a concomitant decrease in cellular thiols. The mRNA level for phospholipid hydroperoxide glutathione peroxidase (PHGPx) was also significantly decreased by ADR administration. Transmission electron microscopic (TEM) observations of testicular germ cells revealed abnormal ultrastructural changes in ADR treated rats. Treatment with lipoic acid (35 mg/kg body weight, i.p.) 1 day prior to ADR administration, effectively reverted these abnormal changes towards normalcy. These findings indicate a cytoprotective role of LA in this experimental model of testicular toxicity.
Prahalathan
Chidambaram
C
Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600113, India.
Selvakumar
Elangovan
E
Varalakshmi
Palaninathan
P
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D004317:Doxorubicin / Q000633:toxicity*; D004336:Drug Antagonism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D008297:Male; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress / Q000187:drug effects; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013090:Spermatocytes / Q000187:drug effects / Q000648:ultrastructure; D013733:Testicular Diseases / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D008063:Thioctic Acid / Q000494:pharmacology*
D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D012333:RNA, Messenger; D008063:Thioctic Acid; D004317:Doxorubicin; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase