title: Targeted DNA transfection into the mouse central nervous system using laser-induced stress waves.
abstract: We investigated the feasibility of gene transfer into the mouse central nervous system (CNS) by applying nanosecond pulsed laser-induced stress waves (LISWs). Intraventricular or hippocampal injection of a reporter gene [enhanced green fluorescent protein (EGFP)] followed by application of LISWs showed this method to be efficient in the CNS of newborn and adult mice. Cells expressing EGFP reside at least 3.5 mm from the surface of the tissue, while no apparent damage was detected. Additionally, expression of EGFP was limited to the area that was exposed to LISWs. Using this method, the formulation of plasmid DNA by cationic transfer reagent polyethylenimine proved to be effective for improving transfer efficiency into the CNS.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Satoh
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Kanda
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Terakawa
authors.forename: Minoru
authors.initials: M
authors.lastname: Obara
authors.forename: Katsushige
authors.initials: K
authors.lastname: Mizuno
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Shogo
authors.initials: S
authors.lastname: Endo
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Ooigawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nawashiro
authors.forename: Shunichi
authors.initials: S
authors.lastname: Sato
authors.forename: Kunio
authors.initials: K
authors.lastname: Takishima
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism* / Q000528:radiation effects; D005240:Feasibility Studies; D018390:Gene Targeting / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism*; D007834:Lasers; D051379:Mice; D010957:Plasmids / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D014162:Transfection / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins