title: Determination of clopidogrel metabolite (SR26334) in human plasma by LC-MS.
abstract: A new, sensitive, specific and reproducible method for determination of clopidogrel metabolite (SR26334) in human plasma has been developed. After liquid-liquid extraction on Chem Elut cartridges with dichloromethane, samples were quantified using reversed-phase high performance liquid chromatography with mass detection. The determination was performed on a Luna C18, 3 microm (75 mmx4.6 mm i.d.) column with an acetonitrile-water-formic acid mixture (60:40:0.1, v/v/v) as a mobile phase. The flow rate was set at 0.2 mL/min. Repaglinide was chosen as an internal standard and the time of analysis was 12 min. For SR26334 the limits of detection and quantification were 7.5 ng/mL and 20 ng/mL, respectively, and the calibration curve was linear up to 3000 ng/mL. The extraction recovery of SR26334 from plasma was within the range of 85-90%. The method has been successfully used to study clopidogrel metabolite pharmacokinetics in healthy volunteers.
authors.affiliation: Department of Pharmacology, Pharmaceutical Research Institute, 8 Rydygiera Street, 01-793 Warsaw, Poland. hksycinska@op.pl
authors.forename: Hanna
authors.initials: H
authors.lastname: Ksycinska
authors.forename: Piotr
authors.initials: P
authors.lastname: Rudzki
authors.forename: Mirosława
authors.initials: M
authors.lastname: Bukowska-Kiliszek
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D006801:Humans; D013058:Mass Spectrometry; D010975:Platelet Aggregation Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013696:Temperature; D013988:Ticlopidine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
chemical_list: D010975:Platelet Aggregation Inhibitors; C521473:SR26334; D013988:Ticlopidine