Mitochondrial abnormalities in inclusion-body myositis.
Mitochondrial changes are frequently encountered in sporadic inclusion-body myositis (s-IBM). Cytochrome c oxidase (COX)-deficient muscle fibers and large-scale mitochondrial DNA (mtDNA) deletions are more frequent in s-IBM than in age-matched controls. COX deficient muscle fibers are due to clonal expansion of mtDNA deletions and point mutations in segments of muscle fibers. Such segments range from 75 microm to more than 1,000 microm in length. Clonal expansion of the 4977 bp "common deletion" is a frequent cause of COX deficient muscle fiber segments, but many other deletions also occur. The deletion breakpoints cluster in a few regions that are similar to what is found in human mtDNA deletions in general. Analysis in s-IBM patients of three nuclear genes associated with multiple mtDNA deletions, POLG1, ANT1 and C10orf2, failed to demonstrate any mutations. In s-IBM patients with high number of COX-deficient fibers, the impaired mitochondrial function probably contribute to muscle weakness and wasting. Treatment that has positive effects in mitochondrial myopathies may be tried also in s-IBM.
Oldfors
A
A
Department of Pathology, Sahlgrenska University Hospital, Göteborg, Sweden. anders.oldfors@pathology.gu.se
Moslemi
A R
AR
Jonasson
L
L
Ohlsson
M
M
Kollberg
G
G
Lindberg
C
C
D000375:Aging / Q000235:genetics; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000032:analysis; D006801:Humans; D008931:Mitochondria, Muscle / Q000201:enzymology / Q000473:pathology* / Q000502:physiology; D017240:Mitochondrial Myopathies / Q000235:genetics; D008969:Molecular Sequence Data; D018485:Muscle Fibers, Skeletal / Q000201:enzymology / Q000473:pathology; D009154:Mutation; D018979:Myositis, Inclusion Body / Q000378:metabolism / Q000473:pathology*; D010085:Oxidative Phosphorylation; D012343:RNA, Transfer / Q000235:genetics; D017384:Sequence Deletion
D004272:DNA, Mitochondrial; D012343:RNA, Transfer; D003576:Electron Transport Complex IV