Apoptosis induction by Epican Forte in HTLV-1 positive and negative malignant T-cells.
The effects of a novel nutrient formulation Epican Forte (EF) were evaluated on proliferation and induction of apoptosis using non-cytotoxic concentrations against HTLV-1 positive (HuT-102 & C91-PL) and negative (CEM & Jurkat) cells. EF showed anti-proliferative effect as determined by MTT assay and TGF mRNA protein expression using RT-PCR. EF resulted in the down-regulation of TGF-alpha and an up-regulation in TGF-beta2. EF caused a significant increase in apoptotic cells in the preG1 phase. These results were confirmed using Cell Death ELISA and Annexin V-FITC. Induction of apoptosis was caused by an up-regulation of p53, p21 and Bax protein levels and a down-regulation of Bcl-2alpha protein expression level.
Harakeh
S
S
Department of Biology, American University of Beirut, Beirut, Lebanon. sharakeh@gmail.com
Diab-Assaf
M
M
Niedzwiecki
A
A
Khalife
J
J
Abu-El-Ardat
K
K
Rath
M
M
D000111:Acetylcysteine / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001120:Arginine / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003300:Copper / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D015368:Human T-lymphotropic virus 1 / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008239:Lysine / Q000494:pharmacology*; D008345:Manganese / Q000494:pharmacology*; D011392:Proline / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012643:Selenium / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000187:drug effects* / Q000821:virology; D015291:Transforming Growth Factors / Q000235:genetics
D000970:Antineoplastic Agents; D008345:Manganese; D015291:Transforming Growth Factors; D003300:Copper; D002392:Catechin; D004247:DNA; D001120:Arginine; D011392:Proline; C045651:epigallocatechin gallate; D012643:Selenium; D008239:Lysine; D001205:Ascorbic Acid; D000111:Acetylcysteine