Serum proteomic signature for cystic fibrosis using an antibody microarray platform.
Antibody microarrays are a new proteomic technology, which we have developed as a platform for identifying a cystic fibrosis (CF)-specific serum proteomic signature. Serum samples from CF patients have been pooled and compared with equivalent pools of control sera in order to identify patterns of protein expression unique to CF. We find that the set of significantly differentially expressed proteins is enriched in protein mediators of inflammation from the NFkappaB signaling pathway, and in proteins that may be selectively expressed in CF-affected tissues such as lung and intestine. In several instances, we validate the data from the antibody microarrays by quantitative analysis with Reverse Capture Protein Microarrays. We conclude that antibody microarray technology is sensitive, quantitative, and robust, and can be useful as a proteomic platform to discriminate between sera from CF and control patients.
Srivastava
Meera
M
Department of Anatomy, Physiology and Genetics, Uniformed Services University School of Medicine, USUHS, Bethesda, MD 20814, USA.
Eidelman
Ofer
O
Jozwik
Catherine
C
Paweletz
Cloud
C
Huang
Wei
W
Zeitlin
Pamela L
PL
Pollard
Harvey B
HB
D000293:Adolescent; D000328:Adult; D000906:Antibodies; D002648:Child; D003550:Cystic Fibrosis / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis*; D044967:Serum
D000906:Antibodies; D020543:Proteome