Determination of 1-nitropyrene metabolites by high-performance liquid chromatography with chemiluminescence detection.
Three metabolites of 1-nitropyrene, i.e., 3-hydoxy-1-nitropyrene (3-OHNP), 6-hydoxy-1-nitropyrene (6-OHNP) and 8-hydoxy-1-nitropyrene (8-OHNP), were sensitively and selectively detected by HPLC with peroxyoxalate chemiluminescence (CL) detection. In the system, the three OHNPs were reduced to their corresponding amino derivatives through a reduction column packed with Pt/Rh-coated alumina and then concentrated on a concentrator column. By rotating a switching valve, the analytes were eluted into a separator column (ODS), separated and then detected by the bis(2,4,6-trichlorphenyl)oxalate-H(2)O(2) CL system. The detection limits (signal-to-noise ratio=3) were in the range from 5fmol (6-OHNP and 8-OHNP) to 12fmol (3-OHNP) per injection. To demonstrate the proposed method, it was used to determine three compounds in the incubation mixture of 1-nitropyrene and rat S9 mix.
Hayakawa
Kazuichi
K
Graduate School of Natural Science and Technology, Faculty of Pharmaceutical Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
Lu
Chao
C
Mizukami
Satoko
S
Toriba
Akira
A
Tang
Ning
N
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008163:Luminescent Measurements; D011721:Pyrenes / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D011721:Pyrenes; C032668:1-nitropyrene