[Alternation of ion transport of airway epithelium by eicosanoids].
BACKGROUND
Airway surface liquid is thought to be regulated by the ion transport processes across the airway epithelium in a vectorial manner. Short circuit current (Isc) measured by Ussing chamber is an indication of electric permeability because of mutually related transportation of amount of ion between apical and basolateral membrane.


METHODS
Guinea pig trachea was mounted on Ussing chamber and short-circuited current was determined continuously. After basolateral application of eicosanoids such as PGD2 and LTC4/LTD4/LTE4, Ramatroban, CRTH2 receptor antagonist and Pranlucast, LT receptor antagonist was added from basolateral side. A typical Cl-channel blocker, NPPB, was added from apical side.


RESULTS
Isc were increased after basolateral application of eicosanoids. These effects were partly inhibited by each antagonist. Cl- channel blocker suppressed effects of each eicosanoids.


CONCLUSIONS
Eicosanoids alter the ion transport mechanism via chloride channels on the airway epithelium that can be blocked by those antagonists.
Nakajima
Noriyuki
N
Department of Oto-rhino-laryngology, Dokkyo Medical University, Koshigaya Hospital. i-tobita@tulip.sannet.ne.jp
Miwa
Masato
M
Watanabe
Kensuke
K
D000818:Animals; D018118:Chloride Channels / Q000037:antagonists & inhibitors; D015194:Diffusion Chambers, Culture; D015777:Eicosanoids / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D017136:Ion Transport / Q000187:drug effects*; D008297:Male; D010539:Permeability; D015230:Prostaglandin D2 / Q000494:pharmacology; D020545:Respiratory Mucosa / Q000187:drug effects*; D013189:SRS-A / Q000494:pharmacology; D014132:Trachea / Q000502:physiology
D018118:Chloride Channels; D015777:Eicosanoids; D013189:SRS-A; D015230:Prostaglandin D2