title: Celsior preserved cardiac mechanoenergetics better than popular solutions in canine hearts.
abstract: BACKGROUND
  Better protective effects of Celsior on cardiac function than the other conventional solutions have been reported in acute experiments and in clinical trials for at-risk patients. However, no study has yet precisely elucidated how these preservation solutions affect cardiac mechanoenergetics. Therefore, we evaluated the effects of St. Thomas' Hospital solution No. 2, University of Wisconsin solution, and Celsior on left ventricular contractility (Emax: end-systolic pressure-volume ratio) and oxygen consumption.
  
  
  METHODS
  We used 32 canine excised cross-circulated hearts. Twenty-three hearts served as donor hearts after hypothermic ischemia with one of the three solutions, and the remaining 9 served as controls. After arrest with each solution, the hearts were preserved for 4 hours at 4 degrees C. Then, we measured left ventricular pressure, volume, and oxygen consumption to obtain Emax and the relation between ventricular pressure-volume area (a measure of total mechanical energy) and oxygen consumption. We also evaluated the oxygen cost of Emax by changing Emax with calcium administration.
  
  
  RESULTS
  Celsior did not significantly affect E(max) (6.3 +/- 2.4 in control versus 5.3 +/- 1.3 mm Hg.mL(-1).100 g with Celsior) nor the oxygen cost of Emax (1.2 +/- 0.6 versus 1.6 +/- 0.5 mL O2.mL.mm Hg(-1).beat(-1).100 g(-2), respectively). In contrast, St. Thomas' Hospital and University of Wisconsin solutions significantly decreased Emax (4.5 +/- 1.1 and 3.5 +/- 0.9 mm Hg.mL(-1).100 g, respectively) and increased the oxygen cost of Emax (2.5 +/- 0.8 and 2.4 +/- 0.9 mL O2.mL.mm Hg(-1).beat(-1).100 g(-2), respectively) compared with control and Celsior-preserved hearts. The slope and intercept of the oxygen consumption versus pressure-volume area relation showed no significant difference among the four groups.
  
  
  CONCLUSIONS
  Celsior showed better protective effects on cardiac mechanoenergetics than St. Thomas' Hospital and University of Wisconsin solutions in the acute phase of heart transplantation.
authors.affiliation: Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Oshima
authors.forename: Satoshi
authors.initials: S
authors.lastname: Mohri
authors.forename: Juichiro
authors.initials: J
authors.lastname: Shimizu
authors.forename: Gentaro
authors.initials: G
authors.lastname: Iribe
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imaoka
authors.forename: Waso
authors.initials: W
authors.lastname: Fujinaka
authors.forename: Takahiko
authors.initials: T
authors.lastname: Kiyooka
authors.forename: Kozo
authors.initials: K
authors.lastname: Ishino
authors.forename: Shunji
authors.initials: S
authors.lastname: Sano
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kajiya
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Suga
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D004187:Disaccharides / Q000494:pharmacology; D004285:Dogs; D004573:Electrolytes / Q000494:pharmacology; D004734:Energy Metabolism; D005971:Glutamates / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006321:Heart / Q000502:physiology*; D016027:Heart Transplantation / Q000379:methods*; D006639:Histidine / Q000494:pharmacology; D008353:Mannitol / Q000494:pharmacology; D009200:Myocardial Contraction; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010101:Oxygen Consumption
chemical_list: C088453:Celsior; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D019188:Organ Preservation Solutions; D008353:Mannitol; D006639:Histidine; D005978:Glutathione