title: Analysis of micrometastatic disease in sentinel lymph nodes from resectable colon cancer: results of Cancer and Leukemia Group B Trial 80001.
abstract: OBJECTIVE
  To determine whether sentinel lymph node (LN) sampling (SLNS) could reduce the number of nodes required to characterize micrometastatic disease (MMD) in patients with potentially curable colon cancer.
  
  
  METHODS
  Cancer and Leukemia Group B 80001 was a study to determine whether SLNS could identify a subset of LNs that predicted the status of the nodal basin for resectable colon cancer and, therefore, could be extensively evaluated for the presence of micrometastases. Patients enrolled onto this study underwent SLNS after injection of 1% isosulfan blue, and both sentinel nodes (SNs) and non-SNs obtained during primary tumor resection were sectioned at multiple levels and stained using anti-carcinoembryonic antigen and anticytokeratin antibodies.
  
  
  RESULTS
  Using standard histopathology, SNs failed to predict the presence of nodal disease in 13 (54%) of 24 node-positive patients. Immunostains were performed for patients whose LNs were negative by standard histopathology. Depending on the immunohistochemical criteria used to assign LN positivity, SN examination resulted in either an unacceptably high false-positive rate (20%) or a low sensitivity for detection of MMD (40%).
  
  
  CONCLUSIONS
  By examining both SNs and non-SNs, this multi-institutional study showed that SNs did not accurately predict the presence of either conventionally defined nodal metastases or MMD. As a result, SLNS is not a useful technique for the study of MMD in patients with colon cancer.
authors.affiliation: Brigham and Women's Hospital, Boston, MA 02115, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Redston
authors.forename: Carolyn C
authors.initials: CC
authors.lastname: Compton
authors.forename: Brent W
authors.initials: BW
authors.lastname: Miedema
authors.forename: Donna
authors.initials: D
authors.lastname: Niedzwiecki
authors.forename: Jeannette M
authors.initials: JM
authors.lastname: Dowell
authors.forename: Scott D
authors.initials: SD
authors.lastname: Jewell
authors.forename: James M
authors.initials: JM
authors.lastname: Fleshman
authors.forename: Jiri
authors.initials: J
authors.lastname: Bem
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Mayer
authors.forename: Monica M
authors.initials: MM
authors.lastname: Bertagnolli
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D003110:Colonic Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000601:surgery*; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008198:Lymph Nodes / Q000473:pathology* / Q000601:surgery*; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D021701:Sentinel Lymph Node Biopsy
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D007633:Keratins