[Determination of ABO blood group genotypes with one tube PCR reaction].
This study was aimed to establish one tube PCR reaction technique to determine ABO blood group genotypes. Salting-out method was adopted to extract genomic DNA; one tube polymerase chain reaction with GeneScan technique was used to identify ABO genotypes. The results showed that the ABO genotypes of 132 samples were in accordance with the phenotypes determined by serological technique. The frequencies of A, B and O were 0.205, 0.159 and 0.636 respectively. AA, AO, AB, BB, BO and OO genotypes were 8 (6.1%), 31 (23.5%), 7 (5.3%), 6 (4.5%), 23 (17.4%), and 57 (43.2%) respectively. It is concluded that one tube polymerase chain reaction with GeneScan technique can determine the genotypes of ABO blood group.
Qin
Fei
F
Blood Center of Zhejiang Province, Hangzhou 310006, China.
He
Ji
J
Zhu
Fa-Ming
FM
Liu
Jin-Hui
JH
Chen
Shu
S
Fu
Qi-Hua
QH
Yan
Li-Xing
LX
D000017:ABO Blood-Group System / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
D000017:ABO Blood-Group System