title: Cell cycle alterations in biopsied olfactory neuroepithelium in schizophrenia and bipolar I disorder using cell culture and gene expression analyses.
abstract: We previously demonstrated that olfactory cultures from individuals with schizophrenia had increased cell proliferation compared to cultures from healthy controls. The aims of this study were to (a) replicate this observation in a new group of individuals with schizophrenia, (b) examine the specificity of these findings by including individuals with bipolar I disorder and (c) explore gene expression differences that may underlie cell cycle differences in these diseases. Compared to controls (n = 10), there was significantly more mitosis in schizophrenia patient cultures (n = 8) and significantly more cell death in the bipolar I disorder patient cultures (n = 8). Microarray data showed alterations to the cell cycle and phosphatidylinositol signalling pathways in schizophrenia and bipolar I disorder, respectively. Whilst caution is required in the interpretation of the array results, the study provides evidence indicating that cell proliferation and cell death in olfactory neuroepithelial cultures is differentially altered in schizophrenia and bipolar disorder.
authors.affiliation: Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, QLD 4111, Australia. richard_mccurdy@camh.net
authors.forename: Richard D
authors.initials: RD
authors.lastname: McCurdy
authors.forename: François
authors.initials: F
authors.lastname: Féron
authors.forename: Chris
authors.initials: C
authors.lastname: Perry
authors.forename: David C
authors.initials: DC
authors.lastname: Chant
authors.forename: Duncan
authors.initials: D
authors.lastname: McLean
authors.forename: Nick
authors.initials: N
authors.lastname: Matigian
authors.forename: Nicholas K
authors.initials: NK
authors.lastname: Hayward
authors.forename: John J
authors.initials: JJ
authors.lastname: McGrath
authors.forename: Alan
authors.initials: A
authors.lastname: Mackay-Sim
mesh_terms: D000328:Adult; D001706:Biopsy; D001714:Bipolar Disorder / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics*; D016923:Cell Death / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D008940:Mitotic Index; D009474:Neurons / Q000473:pathology; D009831:Olfactory Mucosa / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D010716:Phosphatidylinositols / Q000378:metabolism; D012016:Reference Values; D012559:Schizophrenia / Q000235:genetics* / Q000473:pathology; D015398:Signal Transduction / Q000235:genetics
chemical_list: D010716:Phosphatidylinositols