title: Jannaschia seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea.
abstract: A slightly halophilic alphaproteobacterium, designated CL-SP26T, was isolated from hypersaline water of a solar saltern located in Seosin, Korea. 16S rRNA gene sequence analysis revealed an affiliation with the genus Jannaschia. Sequence similarities between CL-SP26T and type strains of members of the genus Jannaschia were 94.9-97.2 %. Cells were rod-shaped and motile with one or more monopolar flagella. Strain CL-SP26T grew on solid media as circular red colonies. It was able to grow in 3-10 % sea salt; however, no growth occurred in media containing NaCl as the only salt. Strain CL-SP26T grew at 5-35 degrees C and pH 7-10. The major fatty acids were 18 : 1omega7c (64 %) and 18 : 0 (12.0 %). Three fatty acids, 3-OH 14 : 0/iso 16 : 1 (3.6 %), 18 : 3omega6c (2.2 %) and 10-methyl 19 : 0 (1.9 %), found in minor quantities in CL-SP26(T), are unique among Jannaschia species. The DNA G+C content was 63 mol%. According to physiological data, fatty acid composition and 16S rRNA gene sequence analysis, CL-SP26T was assigned to the genus Jannaschia, but could be distinguished from recognized species of the genus. Strain CL-SP26T (= KCCM 42114T = JCM 13035T) therefore represents a novel species, for which the name Jannaschia seosinensis sp. nov. is proposed.
authors.affiliation: School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Dong Han
authors.initials: DH
authors.lastname: Choi
authors.forename: Hana
authors.initials: H
authors.lastname: Yi
authors.forename: Jongsik
authors.initials: J
authors.lastname: Chun
authors.forename: Byung Cheol
authors.initials: BC
authors.lastname: Cho
mesh_terms: D020561:Alphaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D003470:Culture Media; D005227:Fatty Acids; D006875:Hydrotherapy; D007723:Korea; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts; D012623:Seawater / Q000382:microbiology*; D017421:Sequence Analysis; D013045:Species Specificity
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012492:Salts