[Effect of Kanglaite injection oncyclooxygenase activity in lung carcinoma A549 cell].
OBJECTIVE
To study the effect of Kanglaite injection on cyclooxygenase activity in lung carcinoma A549 cells.


METHODS
The expression of mRNA of COX-1 and COX-2 were measured by RT-PCR. The expression of COX-2 protein was measured by Western-blot.


RESULTS
Kanglaite injection could selectively decreased COX-2 mRNA expression and protein expression while COX-1 mRNA expression was unchanged.


CONCLUSIONS
Kanglaite injection is selectively inhibitory agent of COX-2, and possess inhibitory effects on cyclooxygenase 2.
Dong
Qing-hua
QH
The 2nd Affiliated Hospital of Zhejiang University, Cancer Institute, Zhejiang University, Hangzhou 310016, China.
Zhong
Xian
X
Zheng
Shu
S
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology / Q000473:pathology; D045744:Cell Line, Tumor; D031742:Coix / Q000737:chemistry; D051545:Cyclooxygenase 1 / Q000096:biosynthesis / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007267:Injections; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology; D010938:Plant Oils / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D010938:Plant Oils; D012333:RNA, Messenger; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2