TP53 gene mutations are rare in nondysplastic Barrett's esophagus.
In search of potential prognostic markers, we analyzed a large series of tissues of Barrett's esophagus and samples of adenocarcinomas arising in the terrain of Barrett's esophagus for TP53 gene mutations by direct sequencing of exons 5 to 9 of the TP53 gene. While 9 of 21 adenocarcinomas tested (42.9%) contained a TP53 mutation, none of 24 samples from Barrett's esophagus were mutated. This observation suggests that TP53 gene mutation may be a relatively late event in the progression from nondysplastic Barrett's esophagus to adenocarcinoma of esophagus. Therefore, TP53 gene mutations alone are not likely to represent a widely useful prognostic marker of the risk of progression to malignancy, at least not in Barrett's esophagus without dysplasia.
Novotna
Kamila
K
Institute of Biology and Medical Genetics, Charles University Second Medical School, 15006, Prague 5, Czech Republic. kamila.novotna@lfmotol.cuni.cz
Trkova
Marie
M
Pazdro
Alexandr
A
Smejkal
Milan
M
Soukupova
Alzbeta
A
Kodetova
Daniela
D
Smejkal
Premysl
P
Sedlacek
Zdenek
Z
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001471:Barrett Esophagus / Q000235:genetics* / Q000473:pathology; D001706:Biopsy; D004247:DNA / Q000235:genetics*; D018450:Disease Progression; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology; D005091:Exons; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology; D012189:Retrospective Studies; D012307:Risk Factors
D005819:Genetic Markers; D004247:DNA