[Isolation and identification of arboviruses from mosquito pools in Yunnan Province].
BACKGROUND
To survey arboviruses in Yunnan province.


METHODS
Mosquitoes were collected from Yunnan Province in 2002 and 2004. Virus strains were isolated by the inoculation of homogenates of the mosquitoes onto BHK cell line. The isolated strains and their molecular biological characteristics were identified by real-time PCR, reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescent antibody technique.


RESULTS
Twelve strains of viruses producing CPE in BHK cells were isolated from 4810 mosquitoes. All the 12 isolates were identified to be Japanese encephalitis viruses. Genotype analysis showed the new virus (DL-0437 strain) belonged to genotype III.


CONCLUSIONS
Twelve strains of Japanese encephalitis viruses were isolated from mosquito pools collected in Yunnan. It was the first isolation of genotype III Japanese encephalitis viruses in Yunnan Province in recent years.
Sun
Xiao-hong
XH
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Fu
Shi-hong
SH
Zhang
Hai-lin
HL
Wang
Huan-yu
HY
He
Ying
Y
Liu
Wei-bin
WB
Yang
Wei-hong
WH
Feng
Yun
Y
Min
Ji-guang
JG
Han
Rui-hong
RH
Liang
Guo-dong
GD
D000818:Animals; D000831:Animals, Newborn; D001103:Arboviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002460:Cell Line; D002681:China; D006224:Cricetinae; D009033:Culicidae / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000821:virology; D005838:Genotype; D008856:Microscopy, Fluorescence; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics
D014759:Viral Envelope Proteins; C074932:prM protein, Flavivirus