title: Mycobacterium avium restriction fragment length polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains.
abstract: Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 AIDS inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns except for one band.
authors.affiliation: Departamento de Micobacterioses, Instituto Oswaldo Cruz-Fiocruz, 21045-900 Rio de Janeiro, RJ, Brazil.
authors.forename: Patrícia Carvalho de
authors.initials: PC
authors.lastname: Sequeira
authors.forename: Leila de Souza
authors.initials: Lde S
authors.lastname: Fonseca
authors.forename: Marlei Gomes da
authors.initials: MG
authors.lastname: Silva
authors.forename: Maria Helena Féres
authors.initials: MH
authors.lastname: Saad
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D001938:Brazil; D002645:Chickens / Q000382:microbiology; D005516:Food Microbiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D009162:Mycobacterium avium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000382:microbiology; D014675:Vegetables / Q000382:microbiology