title: Changes of skeletal muscle adiponectin content in diet-induced insulin resistant rats.
abstract: The current study examined the relationship between skeletal muscle levels of adiponectin and parameters of insulin sensitivity. A high fat/sucrose diet (HFD) for 20 weeks resulted in significant increases in body weight, serum insulin, triglycerides (TG), and free fatty acids (FFA) (all p < 0.01). Interestingly, this diet leads to a slight increase in serum adiponectin, but significant decreases in gastrocnemius muscle and white adipose adiponectin (all p < 0.05). HFD for 4 weeks also resulted in a significant decrease in muscle adiponectin, which correlated with serum insulin, TG, and FFA (all p < 0.05). Treatment of the 4-week HFD rats with a PPARgamma agonist GI262570 ameliorated the diet-induced hyperinsulinemia and dyslipidemia, and effectively restored muscle adiponectin (all p < 0.05). This study demonstrated that HFD-induced hyperinsulinemia and dyslipidemia appeared without changes in serum adiponectin, but were associated with decreased tissue adiponectin. This provides the first evidence for a connection between tissue adiponectin and diet-induced hyperinsulinemia and dyslipidemia.
authors.affiliation: GlaxoSmithKline, Research Triangle Park, NC 27709, USA. baichum.w.yang@gsk.com
authors.forename: Baichun
authors.initials: B
authors.lastname: Yang
authors.forename: Lihong
authors.initials: L
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Qian
authors.forename: James A
authors.initials: JA
authors.lastname: Triantafillou
authors.forename: Judi A
authors.initials: JA
authors.lastname: McNulty
authors.forename: Kevin
authors.initials: K
authors.lastname: Carrick
authors.forename: Lisa G
authors.initials: LG
authors.lastname: Clifton
authors.forename: Bajin
authors.initials: B
authors.lastname: Han
authors.forename: Robert
authors.initials: R
authors.lastname: Geske
authors.forename: Jay
authors.initials: J
authors.lastname: Strum
authors.forename: Kathleen K
authors.initials: KK
authors.lastname: Brown
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Stimpson
authors.forename: Greg
authors.initials: G
authors.lastname: Pahel
mesh_terms: D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D004041:Dietary Fats / Q000378:metabolism*; D019422:Dietary Sucrose / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D010080:Oxazoles / Q000008:administration & dosage; D047495:PPAR gamma / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014443:Tyrosine / Q000008:administration & dosage / Q000031:analogs & derivatives
chemical_list: D052242:Adiponectin; D004041:Dietary Fats; D019422:Dietary Sucrose; D010080:Oxazoles; D047495:PPAR gamma; C422415:farglitazar; D014443:Tyrosine