title: Safety assessment of potential lactic acid bacteria Bifidobacterium longum SPM1205 isolated from healthy Koreans.
abstract: The safety assessment of Bifidobacterium longum SPM1205 isolated from healthy Koreans and this strain's inhibitory effects on fecal harmful enzymes of intestinal microflora were investigated. The overall safety of this strain was investigated during a feeding trial. Groups of SD rats were orally administered a test strain or commercial reference strain B. longum 1 x 10(9) CFU/kg body weight/day for four weeks. Throughout this time, their feed intake, water intake and live body weight were monitored. Fecal samples were periodically collected to test harmful enzyme activities of intestinal microflora. At the end of the four-week observation period, samples of blood, liver, spleen, kidney, and gut tissues were collected to determine for hematological parameters and histological differences. The results obtained in this experiment demonstrated that four weeks of consumption of this Bifidobacterium strain had no adverse effects on rat's general health status, blood biochemical parameters or histology. Therefore, it is likely to be safe for human use. Fecal harmful enzymes such as beta-glucosidase, beta-glucuronidase, tryptophanase and urease, were effectively inhibited during the administration of the B. longum SPM1205. These results suggested that this B. longum SPM 1205 could be used for humans as a probiotic strain.
authors.affiliation: Department of Food Sciencs, Sahmyook College, Gyeonggi-do, Republic of Korea.
authors.forename: Sung Sook
authors.initials: SS
authors.lastname: Choi
authors.forename: Byung Yong
authors.initials: BY
authors.lastname: Kang
authors.forename: Myung Jun
authors.initials: MJ
authors.lastname: Chung
authors.forename: Soo Dong
authors.initials: SD
authors.lastname: Kim
authors.forename: So Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Jung Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Chin Yang
authors.initials: CY
authors.lastname: Kang
authors.forename: Nam Joo
authors.initials: NJ
authors.lastname: Ha
mesh_terms: D000328:Adult; D000818:Animals; D001483:Base Sequence; D001644:Bifidobacterium / Q000302:isolation & purification* / Q000502:physiology*; D001773:Blood Cells; D001774:Blood Chemical Analysis; D001835:Body Weight; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D004326:Drinking; D004435:Eating; D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000382:microbiology; D005966:Glucuronidase / Q000037:antagonists & inhibitors; D006801:Humans; D007723:Korea; D008969:Molecular Sequence Data; D019936:Probiotics / Q000008:administration & dosage* / Q000009:adverse effects*; D051381:Rats; D014368:Tryptophanase / Q000037:antagonists & inhibitors; D014510:Urease / Q000037:antagonists & inhibitors; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D001617:beta-Glucosidase; D005966:Glucuronidase; D014510:Urease; D014368:Tryptophanase