Expression of small hairpin RNAs for S100A9 used in the protein function research.
To study the function of S100A9 protein in HL-60 cells treated with all-trans retinoic acid (ATRA), we had designed and constructed retroviral vectors for expression of small hairpin RNAs (shRNAs), and silenced S100A9 expression in HL-60 cells treated with ATRA. The silence efficiency of siRNA was detected with RT-PCR and Western blotting. The differentiation of HL-60 was monitored by nitro blue tetrazolium (NBT) reduction experiment. Western blot showed that shRNAs remarkably reduce of S100A9 expression in HL-60 cells when they were induced to differentiation by ATRA. But NBT positive percentage of differentiated HL-60 cells was no remarked change with S100A9 expression. The data indicated S100A9 could be no important action during differentiation of HL-60 treated with ATRA.
Mou
Zhirong
Z
Institute of Immunology, PLA, Third Military Medical University, 30 Gaotanyan, Chongqing 400038, PR China.
Zhou
Jinran
J
Li
Jintao
J
Zou
Liyun
L
Zhou
Wei
W
Wu
Yuzhang
Y
D040502:Calgranulin B / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D020869:Gene Expression Profiling; D020868:Gene Silencing / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship; D014212:Tretinoin / Q000494:pharmacology
D040502:Calgranulin B; D034741:RNA, Small Interfering; D014212:Tretinoin