title: Double-stranded RNA viral infection in Cuban Trichomonas vaginalis isolates.
abstract: Trichomonas vaginalis can be infected with double-stranded RNA (dsRNA) viruses designated T. vaginalis virus (TVV), which may have important implications for trichomonal virulence and disease pathogenesis. We tested for TVV in 40 fresh T. vaginalis isolates from Cuban patients by total extraction of nucleic acids (DNA and RNA). TVV was detected in 22 (55%) of the 40 T. vaginalis isolates. This gives an estimate of the infection rate of Cuban T. vaginalis isolates by the dsRNA virus. Future research should focus on the association between trichomonosis symptoms and the presence of TVV.
authors.affiliation: Department of Parasitology, Institute of Tropical Medicine, Havana, Cuba. fraga@ipk.sld.cu
authors.forename: Jorge
authors.initials: J
authors.lastname: Fraga
authors.forename: Lázara
authors.initials: L
authors.lastname: Rojas
authors.forename: Idalia
authors.initials: I
authors.lastname: Sariego
authors.forename: Aymé
authors.initials: A
authors.lastname: Fernández-Calienes
mesh_terms: D000293:Adolescent; D000818:Animals; D003462:Cuba; D004279:DNA, Viral / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis; D014247:Trichomonas Vaginitis / Q000821:virology*; D014246:Trichomonas vaginalis / Q000302:isolation & purification / Q000472:pathogenicity / Q000821:virology*
chemical_list: D004279:DNA, Viral; D012330:RNA, Double-Stranded; D012367:RNA, Viral