Smad3-null mice lack interstitial cells of Cajal in the colonic wall.
BACKGROUND
Transforming growth factor-beta (TGF-beta)/Smad's signalling pathway plays a pivotal role in organogenesis, oncogenesis, inflammation, repair and fibrosis. The aim of this study was to evaluate the morphology of muscle layers and the density and distribution of interstitial cells of Cajal (ICC) in the colon of Smad3 knockout mice.


METHODS
Eighteen Smad3 wild-type mice and 12 null mice were sacrificed at age 4 months and the colons were collected for histology (Haematoxilin-Eosin, Masson thrichrome and Gomori silver staining), morphometry and immunohistochemistry (IHC) analysis. For IHC we used the c-Kit, alpha-smooth muscle actine (alpha-SMA), vimentin, desmin and neuronal cocktail (S-100, NSE, neurofilament 200) antibodies.


RESULTS
When sacrificed, 40% of the null mice showed different degrees of colon dilatation when compared with the wild-type. Histological and morphometric evaluation revealed a significant reduction in muscle layer thickness of the colon in all the null mice when compared with the wild-type. Immunohistochemistry evaluation showed a marked reduction, or even absence, of c-Kit immunoreactivity, which identifies ICC, in the colon of all the null mice, compared with the wild-type.


CONCLUSIONS
Smad3 null mice showed a marked reduction, or even absence, of ICC in the colon together with a concomitant reduction of intestinal smooth muscle layer thickness. This data could account for the colonic dilation observed in approximately 40% of the Smad3 null mice. Alteration of intestinal smooth muscle layers and ICC could also be involved in the resistance of the Smad3 null mice to develop colonic fibrosis.
Vetuschi
A
A
Università degli Studi di L'Aquila, L'Aquila, Italy.
Sferra
R
R
Latella
G
G
D'Angelo
A
A
Catitti
V
V
Zanninelli
G
G
Continenza
M A
MA
Gaudio
E
E
D000818:Animals; D020541:Coiled Bodies / Q000378:metabolism / Q000473:pathology*; D003106:Colon / Q000378:metabolism / Q000473:pathology*; D004108:Dilatation, Pathologic / Q000378:metabolism / Q000473:pathology; D007124:Immunoenzyme Techniques; D051379:Mice; D018345:Mice, Knockout; D009124:Muscle Proteins / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism / Q000473:pathology*; D010641:Phenotype; D015398:Signal Transduction; D051900:Smad3 Protein / Q000235:genetics / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000502:physiology
D009124:Muscle Proteins; D051900:Smad3 Protein; D016212:Transforming Growth Factor beta