title: Role of matrix metalloproteinase-7 in the modulation of a Chlamydia trachomatis infection.
abstract: To determine the role of matrix metalloproteinase-7 (MMP-7) in the pathogenesis of chlamydial infection, C57BL/6 wild-type (WT) and MMP-7 knockout (KO) mice were infected intravaginally with Chlamydia trachomatis mouse pneumonitis (MoPn). Over a period of 6 weeks postinfection, various organs were cultured for C. trachomatis. Other infected animals were mated to assess their fertility status. No significant differences were observed between WT and KO mice in the number of animals with positive vaginal cultures, length of time of C. trachomatis shedding, or the number of C. trachomatis inclusion-forming units (IFU) recovered from their genital tracts. Likewise, the number of animals with hydrosalpinx, and the fertility rates and the number of embryos per mouse, were similar in WT and KO mice. Cultures from the spleen, lungs, kidneys and large intestine yielded similar numbers of IFU from WT and KO mice. However, the number of C. trachomatis IFU recovered from the small intestine of KO mice was significantly higher than that recovered from the small intestine of WT mice at 2 weeks postinfection. Because MMP-7 KO mice are deficient in active intestinal alpha-defensins, the results suggest that these components play a role in regulating colonization of the gastrointestinal tract by Chlamydia. By contrast, MMP-7 is dispensable in the progression and resolution of the genital tract infection.
authors.affiliation: Department of Pathology, Medical Sciences I, University of California, Irvine, 92697, USA.
authors.forename: Sukumar
authors.initials: S
authors.lastname: Pal
authors.forename: Amy P
authors.initials: AP
authors.lastname: Schmidt
authors.forename: Ellena M
authors.initials: EM
authors.lastname: Peterson
authors.forename: Carole L
authors.initials: CL
authors.lastname: Wilson
authors.forename: Luis M
authors.initials: LM
authors.lastname: de la Maza
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D002690:Chlamydia Infections / Q000201:enzymology* / Q000276:immunology; D002692:Chlamydia trachomatis / Q000276:immunology / Q000302:isolation & purification*; D018450:Disease Progression; D005260:Female; D005831:Genital Diseases, Female / Q000201:enzymology* / Q000276:immunology; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007247:Infertility, Female / Q000276:immunology / Q000382:microbiology; D020783:Matrix Metalloproteinase 7 / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009924:Organ Culture Techniques; D014621:Vagina / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D020783:Matrix Metalloproteinase 7