On tracks and locomotives: the long route of DNA to the nucleus.
Molecular motors have prominent functions in organelle transport, cytoskeletal organization, division and motility. The dyneins are one of the three families of cytoskeleton-based molecular motors and they travel along the cytoplasmic microtubule network towards the minus end of the microtubule. This directed movement is used by DNA viruses to deliver their infectious genome and proteins to the host cell nucleus. In recent studies, it has been hypothesized that Agrobacterium species use a similar pathway to deliver their infectious unit--a large complex between single-stranded DNA and proteins--to the host cell nucleus and that a karyophilic protein carrier that can deliver synthetic DNA to the nucleus is also driven by a dynein motor. These studies shed light on the mechanism of Agrobacterium-mediated genetic transformation and could lead to new methods for the efficient transfection of synthetic DNA.
Tzfira
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Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA. ttzfira@umich.edu
D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology*; D004279:DNA, Viral / Q000378:metabolism*; D004398:Dyneins / Q000502:physiology*; D005813:Genes, Synthetic; D008870:Microtubules / Q000502:physiology; D008954:Models, Biological; D012231:Rhizobium / Q000235:genetics*; D014162:Transfection / Q000379:methods
D004279:DNA, Viral; D004398:Dyneins