Generic bioaffinity silicone surfaces.
Synthetic polymer surfaces require surface modification to improve biocompatibility. A generic route to biocompatible silicone elastomers is described involving high yield surface functionalization of standard silicones with hydrosilanes, hydrosilylation using asymmetric, allyl-, NSC-terminated PEO of narrow molecular weight, and covalent modification in one step with amine-containing biological molecules including oligopeptides (YIGSR, RGDS), proteins (EGF, albumin, fibrinogen, mucin), and glycosaminoglycans (heparin). Efficient, high-density binding (e.g., 0.2 EGF molecules/nm2) was demonstrated using radiolabeling studies. The resulting surfaces were demonstrated to be biocompatible by further reaction with biomolecules, for example, thrombosis suppression on surfaces modified by heparin + ATIII, and the formation of confluent corneal epithelial cell layers on EGF, RGDS, or YIGSR surfaces.
Chen
Hong
H
Department of Chemical Engineering, McMaster University, 1280 Main Street W., Hamilton ON Canada, L8S 4M1.
Brook
Michael A
MA
Sheardown
Heather D
HD
Chen
Yang
Y
Klenkler
Bettina
B
D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002254:Carbonates / Q000737:chemistry*; D002448:Cell Adhesion; D002478:Cells, Cultured; D003315:Cornea; D004847:Epithelial Cells / Q000502:physiology; D006493:Heparin / Q000737:chemistry; D006801:Humans; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D012826:Silicone Elastomers / Q000138:chemical synthesis / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry*
D001672:Biocompatible Materials; D002254:Carbonates; D009842:Oligopeptides; D011506:Proteins; D012826:Silicone Elastomers; D013388:Succinimides; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; C085786:succinimidyl carbonate; D011092:Polyethylene Glycols; D006493:Heparin; C046617:arginyl-glycyl-aspartyl-serine