Vitamin E inhibits hemolysis induced by hemin as a membrane stabilizer.
Hemin is a potential cytolytic agent. To test the effect of vitamin E on hemin-mediated permeability in cell membranes, sheep erythrocytes were chosen as an appropriate model to study hemolysis induced by hemin. Hemin-induced hemolysis but did not elicit lipid peroxidation in sheep erythrocytes. Vitamin E was effective in inhibiting hemin-mediated hemolysis. Both chromanol ring and the isoprenoid side chain of tocopherols were essential for inhibition of hemin-induced hemolysis. There was a strong correlation between the inhibitory effects of tocopherols on hemin-induced erythrocyte hemolysis and their effects on fluorescence anisotropy of cell membranes. Our results suggested that, in contrast to its antioxidant activity, vitamin E inhibits hemolysis induced by hemin as a membrane stabilizing agent.
Wang
Fang
F
School of Life Sciences, Yunnan University, Kunming, Yunnan 650091, China.
Wang
Tinghua
T
Lai
Jianhua
J
Li
Ming
M
Zou
Chenggang
C
D000818:Animals; D016880:Anisotropy; D000975:Antioxidants / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D004912:Erythrocytes / Q000187:drug effects* / Q000473:pathology; D005454:Fluorescence Polarization; D006427:Hemin / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D008560:Membrane Fluidity / Q000187:drug effects; D012756:Sheep; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D014810:Vitamin E; D006427:Hemin