Arrays of transfected mammalian cells for high content screening microscopy.
In this chapter we describe protocols for reverse transfection to generate mammalian cell arrays for systematic gene knock-downs by RNAi or knock-ins by ectopic cDNA expression. The method is suitable for high content screening microscopy at a high spatial and temporal resolution allowing even time-lapse analysis of hundreds of samples in parallel.
Erfle
Holger
H
Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany.
Pepperkok
Rainer
R
D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D018390:Gene Targeting; D006801:Humans; D020527:Microscopy, Scanning Probe; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection / Q000379:methods*
D018076:DNA, Complementary; D034741:RNA, Small Interfering