Triage test and HPLC assay for analysis of vegetamin, an antipsychotic agent, using gastric contents and blood specimens.
Vegetamin is an antipsychotic agent composed of phenobarbital, promethazine and chlorpromazine. While phenobarbital can be detected by the Triage kit for screening drugs of abuse, the other two components cannot. We describe here the detection of promethazine and chlorpromazine using either a high performance liquid chromatography (HPLC) unit equipped with an ultraviolet, a photodiode array (PDA), or an electrochemical detector (ECD), or gas chromatograph-mass spectrometry (GC-MS). The absorption and mass spectra of promethazine and chlorpromazine were nonspecific, and thus the ECD-HPLC system was best equipped to quantitate these components. Comparison of the chromatograms of blood and gastric contents aids the identification of drug metabolites.
Yoshida
Manabu
M
Department of Legal Medicine, Kansai Medical University, Moriguchi, Japan. yoshidm@takii.kmu.ac.jp
Akane
Atsushi
A
Nishikawa
Mayumi
M
Tsuchihashi
Hitoshi
H
D014150:Antipsychotic Agents / Q000032:analysis*; D002746:Chlorpromazine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D005766:Gastrointestinal Contents / Q000737:chemistry*; D006801:Humans; D010634:Phenobarbital / Q000032:analysis*; D011398:Promethazine / Q000032:analysis*; D015813:Substance Abuse Detection / Q000379:methods
D014150:Antipsychotic Agents; D004338:Drug Combinations; C515390:vegetamin; D011398:Promethazine; D002746:Chlorpromazine; D010634:Phenobarbital