title: Sarpogrelate hydrochloride, an antagonist of 5-hydroxytryptamine receptor, improves skin flap survival and suppresses thrombus formation in injured microvessels of rabbits.
abstract: The authors carried out two studies to examine the effects of the 5-hydroxytryptamine receptor antagonist, sarpogrelate hydrochloride (SH), on flap necrosis. The first study measured survival rates and included histologic examination of random-pattern skin flaps in rabbits. The second study assessed the time to complete obstruction of blood flow with the artificial thrombus formation method, and the time for leukocytes to adhere to the endothelium of microvessels in a rabbit-ear chamber. The treatment groups were injected with SH. The survival rates of skin flaps in the SH-treated group were increased significantly. Histologic examination of vessels in the control group revealed that vessels in the deep dermis were obstructed completely by thrombi, whereas such vessels were not obstructed in the SH-treated group. Thrombus formation time and leukocyte adhesion time in the SH-treated group were prolonged and decreased, respectively. Thus, SH maintained flap circulation and promoted flap survival by preventing thrombus formation.
authors.affiliation: Department of Plastic and Aesthetic Surgery, School of Medicine, University of Tokushima, Tokushima, Japan.
authors.forename: Maki Toda
authors.initials: MT
authors.lastname: Takase
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hashimoto
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakaya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Harada
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Sedo
authors.forename: Ikuno
authors.initials: I
authors.lastname: Kanda
mesh_terms: D000818:Animals; D001160:Arterioles / Q000187:drug effects / Q000293:injuries / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D004423:Ear / Q000098:blood supply; D006085:Graft Survival / Q000187:drug effects*; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D011817:Rabbits; D012702:Serotonin Antagonists / Q000494:pharmacology*; D013386:Succinates / Q000494:pharmacology*; D013524:Surgical Flaps; D013927:Thrombosis / Q000517:prevention & control*; D014654:Vascular Patency / Q000187:drug effects; D014699:Venules / Q000187:drug effects / Q000293:injuries / Q000473:pathology
chemical_list: D012702:Serotonin Antagonists; D013386:Succinates; C064294:sarpogrelate