title: Dimensional assessment of X-bearing and Y-bearing haploid and disomic human sperm with the use of fluorescence in situ hybridization and objective morphometry.
abstract: OBJECTIVE
  To explore the dimensional attributes of haploid and disomic X-bearing and Y-bearing spermatozoa.
  
  
  METHODS
  Morphometric evaluation of more than 2,000 X-bearing and Y-bearing spermatozoa after identification of the genotype with fluorescence in situ hybridization.
  
  
  METHODS
  Academic clinical and research andrology laboratory.
  
  
  METHODS
  Sperm head area, perimeter, long axis, short axis, shape factor, elliptical form factor (long axis/short axis), and tail length.
  
  
  RESULTS
  We found no differences in dimensions or dimensional distributions between X-bearing and Y-bearing spermatozoa, whether in the native or the decondensed state, or in oligozoospermic or normozoospermic men. There were inconsistent differences and a 70% overlap in the dimensions of haploid and disomic spermatozoa. The other 30% of sperm with disomic nuclei were either smaller or larger compared to haploid spermatozoa.
  
  
  CONCLUSIONS
  There are no differences, or distinguishing characteristics, in dimensions or dimensional distributions between X-bearing and Y-bearing spermatozoa. Dimensional attributes do not discriminate between dysomic and haploid spermatozoa.
authors.affiliation: Department of Obstetrics and Gynecology, Szent-Györgi University School of Medicine, Szeged, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Zavaczki
authors.forename: Ciler
authors.initials: C
authors.lastname: Celik-Ozenci
authors.forename: Laszlo
authors.initials: L
authors.lastname: Ovari
authors.forename: Attila
authors.initials: A
authors.lastname: Jakab
authors.forename: G Leyla
authors.initials: GL
authors.lastname: Sati
authors.forename: David C
authors.initials: DC
authors.lastname: Ward
authors.forename: Gabor
authors.initials: G
authors.lastname: Huszar
mesh_terms: D048430:Cell Shape; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D005838:Genotype; D006238:Haploidy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008858:Microscopy, Phase-Contrast; D009845:Oligospermia / Q000473:pathology; D013077:Sperm Head; D013082:Sperm Tail; D013094:Spermatozoa / Q000648:ultrastructure*; D024182:Uniparental Disomy