title: High performance liquid chromatographic determination of isoniazid, pyrazinamide and rifampicin in pharmaceutical preparations.
abstract: lsoniazid (IS) individually or in the presence of pyrazinamide and rifampicin has been determined by high performance liquid chromatography (HPLC) after derivatization with 6-methyl-2-pyridinecarboxaldehyde. Pyrazinamide and rifampicin separate completely from isoniazid derivative and are determined simultaneously. The chromatography is carried out from YMC-ODS column with elution with methanol: water: isopropanol: acetonitrile: sodium acetate (1 mM) 51:42:3:2:2: v/v/v/v/v/) with flow rate 1.7 ml/min and UV detection at 333 nm. The method is applied for the analysis of Isoniazid B.P Rambuzid and Myrene-p tablets.
authors.affiliation: Institute of Chemistry, University of Sindh, Jamshoro.
authors.forename: M Y
authors.initials: MY
authors.lastname: Khuhawar
authors.forename: F M
authors.initials: FM
authors.lastname: Rind