Cytokines produced by T cells in adenoid surface secretion are mainly downregulatory or of Th1 type.
CONCLUSIONS
T cells in adenoid surface secretion (AdSS) possess the property of cytokine production and are mainly downregulatory or of Th1 type. These results strengthen the hypothesis of an active immunological defense in AdSS.


OBJECTIVE
Recently, we demonstrated the presence of activated T cells in AdSS. The aims of this study were to elucidate the ability of these T cells to produce cytokines and to reveal their cytokine profile.


METHODS
Eight children subjected to adenoidectomy were enrolled. Samples of AdSS, adenoid tissue and peripheral blood were obtained, as well as a nasopharyngeal culture. T cells obtained from AdSS, adenoid tissue and peripheral blood were then cultured and stimulated with anti-CD3 and -CD28 for 5 days. The production of interferon (IFN)-gamma, tumor necrosis factor (TNF)-a and IL-2, -4, -5 and -10 was then analyzed using flow cytometry.


RESULTS
All children had T cells in AdSS capable of cytokine production. T cells in AdSS, adenoid tissue and peripheral blood samples from all children produced IFN-gamma. Of the cytokines analyzed, IFN-gamma was produced in the highest concentrations. In 6/8 children, T cells in AdSS also produced TNF-a and IL-10.
Ivarsson
M
M
Department of Otorhinolaryngology, Sahlgrenska University Hospital, Göteborg, Sweden. Magnus.Ivarsson@orlss.gu.se
Lundin
B S
BS
D000233:Adenoidectomy; D000234:Adenoids / Q000276:immunology* / Q000378:metabolism; D018929:Cell Culture Techniques; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis*; D015536:Down-Regulation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis; D016753:Interleukin-10 / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D009092:Mucous Membrane / Q000378:metabolism; D015508:Nasal Obstruction / Q000209:etiology / Q000601:surgery; D009305:Nasopharynx / Q000382:microbiology; D010034:Otitis Media with Effusion / Q000150:complications / Q000276:immunology / Q000601:surgery; D013601:T-Lymphocytes / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology
D016207:Cytokines; D016753:Interleukin-10; D007371:Interferon-gamma