Secreted antigens of Mycobacterium avium subspecies paratuberculosis as prominent immune targets.
We here describe the identification and characterization of three novel secreted Mycobacterium avium subsp. paratuberculosis antigens of 9, 15 and 34 kDa (Map2609, Map2942c and Map0210c, respectively) by screening a genomic expression library with a serum of a naturally infected clinical cow. The 9, 15 and 34 kDa antigens display strong homology to previously described M. tuberculosis antigens, TB8.4, MPT53 and Erp, respectively. Furthermore, these antigens were shown to be recognized by antibodies from infected cattle, when tested with a limited number of sera from subclinical (n=7) and clinical (n=3) infected cattle.
Willemsen
Peter T J
PT
Department of Bacteriology & TSEs, Central Institute for Animal Disease Control, CIDC-Lelystad, P.O. Box 2004, Edelhertweg 15, 8203 AA Lelystad, The Netherlands. peter.willemsen@wur.nl
Westerveen
José
J
Dinkla
Annemieke
A
Bakker
Douwe
D
van Zijderveld
Fred G
FG
Thole
Jelle E R
JE
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology* / Q000378:metabolism; D010283:Paratuberculosis / Q000276:immunology* / Q000382:microbiology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial