Pseudozyma hubeiensis sp. nov. and Pseudozyma shanxiensis sp. nov., novel ustilaginomycetous anamorphic yeast species from plant leaves.
Among basidiomycetous yeast strains isolated from wilting leaves of various plants in China, two groups of Pseudozyma strains were distinguished from the others by morphological and physiological characterization. Molecular taxonomic analysis based on sequencing of the large subunit (26S) rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region confirmed that the two groups represent two novel species. They are proposed as Pseudozyma hubeiensis sp. nov. (type strain WS 6.4T = AS 2.2493T = CBS 10077T) and Pseudozyma shanxiensis sp. nov. (type strain SH 64T = AS 2.2523T = CBS 10075T). The phylogenetic relationships of the novel species with described Pseudozyma species and related ustilagomycetous teleomorphs were analysed based on the combined sequences of ITS and D1/D2. The phenotypic diagnosis of Pseudozyma was emended because of the negative inositol assimilation reaction of P. hubeiensis sp. nov., which was closely related to the type species of the genus, Pseudozyma prolifica.
Wang
Qi-Ming
QM
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Jia
Jian-Hua
JH
Bai
Feng-Yan
FY
D002681:China; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000382:microbiology; D010944:Plants / Q000382:microbiology*; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014587:Ustilaginales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S; C065096:RNA, ribosomal, 26S