title: Functional analysis of EID1, an F-box protein involved in phytochrome A-dependent light signal transduction.
abstract: Empfindlicher im Dunkelroten Licht 1 (EID1) is an F-box protein that functions as a negative regulator in phytochrome A (phyA)-specific light signalling. F-box proteins are components of SCF ubiquitin ligase complexes that target proteins for degradation in the proteasome. Here we present further characterization of EID1 at the expression level, and show that it regulates photomorphogenesis in seedlings, rosette leaf development and flowering. Data on transcript expression patterns indicate that EID1 is expressed during all stages of Arabidopsis development and exhibits no light response. Microscope studies demonstrate that EID1 is localized to the nucleus, where it can form speckles under continuous far-red light that resemble clastosomes. To characterize the composition and formation of SCF(EID1) complexes further, we used two-hybrid and bridge assays in yeast and in planta. EID1 interacts specifically with several Arabidopsis Skp1-like (ASK) proteins and Cullin1 to form stable dimeric and trimeric complexes. Our results support a two-step association process in which the F-box protein binds first to the ASK adaptor, forming a unit which then associates with the catalytic core of the SCF complex. Finally, our data indicate that the EID1 target interaction domain is composed of two independent modules.
authors.affiliation: Albert-Ludwigs-Universität Freiburg i. Br., Institut für Biologie 2 - Botanik, Schänzlestr. 1, 79104 Freiburg i. Br., Germany.
authors.forename: Katia
authors.initials: K
authors.lastname: Marrocco
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Erik
authors.initials: E
authors.lastname: Bury
authors.forename: Monika
authors.initials: M
authors.lastname: Dieterle
authors.forename: Markus
authors.initials: M
authors.lastname: Funk
authors.forename: Pascal
authors.initials: P
authors.lastname: Genschik
authors.forename: Martina
authors.initials: M
authors.lastname: Krenz
authors.forename: Thorsten
authors.initials: T
authors.lastname: Stolpe
authors.forename: Thomas
authors.initials: T
authors.lastname: Kretsch
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D035264:Flowers / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D008027:Light; D008856:Microscopy, Fluorescence / Q000379:methods; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051942:Phytochrome A / Q000378:metabolism*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D036226:Seedlings / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C426104:EID1 protein, Arabidopsis; D044783:F-Box Proteins; D009687:Nuclear Proteins; C497973:PHYA protein, Arabidopsis; D051942:Phytochrome A; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins