Chemiluminescence enzyme immunoassay (CLEIA) for the determination of chloramphenicol residues in aquatic tissues.
A competitive indirect chemiluminescence enzyme immunoassay (ic-CLEIA) for chloramphenicol (CAP) residues in shrimp has been developed. After optimization (incubation time, concentration of Tween-20, concentration of PBS and pH), the method gave a limit of detection of 0.01 ng/mL and a detection range of 0.03-23.7 ng/mL, with an ED(50) of 0.47 ng/mL. The method has been validated on spiked shrimp samples in terms of precision (intra- and interassay coefficient variations of less than 10% and 15%, respectively) and accuracy (mean recovery 95-123%). The assay performance is better than the ELISA method which is widely used to detect chloramphenicol and indicates that the CLEIA method can be used to test aquatic samples instead of ELISA.
Chuanlai
Xu
X
School of Food Science and Technology, Southern Yangtze University, Wuxi, Jiang Su 214036, People's Republic of China. xcl@sytu.edu.cn
Cifang
Peng
P
Kai
Hao
H
Zhengyu
Jin
J
Wukang
Wang
W
D000818:Animals; D002701:Chloramphenicol / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D033561:Penaeidae / Q000737:chemistry*; D012680:Sensitivity and Specificity
D002701:Chloramphenicol