Molecular markers detecting an ectomycorrhizal Suillus collinitus strain on Pinus halepensis roots suggest successful inoculation and persistence in Mediterranean nursery and plantation.
Survival of the ectomycorrhizal fungal strain Suillus collinitus Sc-32 on Pinus halepensis after inoculation and outplanting was monitored in a Mediterranean plantation. Three molecular fingerprints were developed: RFLP of the internal transcribed spacer ribosomal DNA, intersimple sequence repeat, and a specific sequence-characterized amplified region marker. The inoculant was demonstrated to survive on inoculated seedlings 4 years after outplanting (56 months after inoculation), although S. collinitus was not fruiting. The designed markers set allows reliable and inexpensive monitoring of inoculated seedlings and suggests that S. collinitus is suitable for inoculation of Mediterranean Pinus. These data are discussed in the framework of suilloid population ecology.
El Karkouri
Khalid
K
UMR INRA-Agro.M Rhizosphère & Symbiose, Montpellier Cedex 1, France. khalid.2@wanadoo.fr
Selosse
Marc-André
MA
Mousain
Daniel
D
D000383:Agriculture; D015415:Biomarkers / Q000032:analysis; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004784:Environmental Monitoring; D019083:Mediterranean Region; D038821:Mycorrhizae / Q000235:genetics / Q000302:isolation & purification*; D028223:Pinus / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D036226:Seedlings / Q000382:microbiology; D013045:Species Specificity
D015415:Biomarkers; D017931:DNA Primers; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer