A novel transposon for functional expression of DNA libraries.
Environmental DNA libraries are important sources for novel biocatalyst genes but activity screening for relevant enzymes is often inefficient. Therefore, we have constructed the transposon MuExpress which randomly integrates in vitro into existing bacterial artificial chromosome (BAC) or cosmid libraries and permits the inducible expression of its flanking regions in both directions. Furthermore, this transposon allows the bidirectional sequencing of the respective clones starting from unique primer binding sites.
Leggewie
Christian
C
Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Duesseldorf, Forschungszentrum Juelich, D-52426 Juelich, Germany.
Henning
Helge
H
Schmeisser
Christel
C
Streit
Wolfgang R
WR
Jaeger
Karl-Erich
KE
D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015723:Gene Library; D005818:Genetic Engineering / Q000379:methods*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial