title: Secreted antigens of Mycobacterium avium subspecies paratuberculosis as prominent immune targets.
abstract: We here describe the identification and characterization of three novel secreted Mycobacterium avium subsp. paratuberculosis antigens of 9, 15 and 34 kDa (Map2609, Map2942c and Map0210c, respectively) by screening a genomic expression library with a serum of a naturally infected clinical cow. The 9, 15 and 34 kDa antigens display strong homology to previously described M. tuberculosis antigens, TB8.4, MPT53 and Erp, respectively. Furthermore, these antigens were shown to be recognized by antibodies from infected cattle, when tested with a limited number of sera from subclinical (n=7) and clinical (n=3) infected cattle.
authors.affiliation: Department of Bacteriology & TSEs, Central Institute for Animal Disease Control, CIDC-Lelystad, P.O. Box 2004, Edelhertweg 15, 8203 AA Lelystad, The Netherlands. peter.willemsen@wur.nl
authors.forename: Peter T J
authors.initials: PT
authors.lastname: Willemsen
authors.forename: José
authors.initials: J
authors.lastname: Westerveen
authors.forename: Annemieke
authors.initials: A
authors.lastname: Dinkla
authors.forename: Douwe
authors.initials: D
authors.lastname: Bakker
authors.forename: Fred G
authors.initials: FG
authors.lastname: van Zijderveld
authors.forename: Jelle E R
authors.initials: JE
authors.lastname: Thole
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology* / Q000378:metabolism; D010283:Paratuberculosis / Q000276:immunology* / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial