[High-throughput screening assay for groups of effective components extracted from Xiaoxuming Recipe].
OBJECTIVE
To present a new approach for the research of effective components extracted from compounds of traditional Chinese medicine and their action mechanisms by high-throughput screening assay.


METHODS
We observed the anti-oxidation activities of 240 sequential components (L1-L120 and A1-A120) extracted from Xiaoxuming Recipe, the effects of these components on SH-SY5Y cells with H(2)O(2)-induced and L-glutamic acid-induced damages and the levels of resting calcium ion in neurocytes of rats.


RESULTS
Some components (L1-L40, A100-A120) extracted from Xiaoxuming Recipe had the corresponding effects listed above. The combination of these components was regarded as the groups of effective components of Xiaoxuming Recipe in treating sequelae resulting from brain ischemia.


CONCLUSIONS
Xiaoxuming Recipe has protective effect on brain-ischemia-induced damages through the actions of multiple components with multiple targets.
Wang
Yue-Hua
YH
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Zhang
Hai-Xia
HX
Li
Qi
Q
Ding
Yi
Y
Hu
Juan-Juan
JJ
Du
Guan-Hua
GH
D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002545:Brain Ischemia / Q000150:complications; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology*; D009474:Neurons / Q000473:pathology; D051381:Rats; D015427:Reperfusion Injury / Q000517:prevention & control*; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D004365:Drugs, Chinese Herbal