title: Immobilization of aminoglycosidic aminocyclitols antibiotic onto soap-free poly(MMA-EA-AA) latex particles.
abstract: Monodispersed soap-free poly(MMA-EA-AA) latex particles with surface carboxyl groups were synthesized by emulsion polymerization of methyl methacrylate (MMA), ethyl acrylate (EA) and acrylic acid (AA) in aqueous medium, and streptomycin sulfate (SMS) was immobilized onto these particles using three different methods. A model experiment was designed to test the feasibility of the reaction between the carboxyl groups of polymer and the amino groups of the medicine. The covalent coupling between the latex particles and the medicine was confirmed by XPS. Results showed that the medicine molecules were located on the particle surface after immobilization, and the coupling efficiency of SMS in pre-adsorption method was higher than that in direct method. The highest coupling efficiency of this medicine was achieved using the spacer-arm method. It was demonstrated that the immobilized medicine had similar antimicrobial activity as the free form using Escherichia coli as an evaluating organism.
authors.affiliation: Department of Chemical Engineering, School of Materials Science and Engineering, Tsinghua University, Beijing, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Kang
authors.forename: Chengyou
authors.initials: C
authors.lastname: Kan
authors.forename: Yi
authors.initials: Y
authors.lastname: Du
authors.forename: Deshan
authors.initials: D
authors.lastname: Liu
authors.forename: Anthony
authors.initials: A
authors.lastname: Yeung
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000617:Aminoglycosides / Q000737:chemistry* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D003500:Cyclization; D003902:Detergents; D004926:Escherichia coli / Q000187:drug effects; D007840:Latex / Q000737:chemistry*; D020366:Methylmethacrylate / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D008956:Models, Chemical; D015394:Molecular Structure; D013057:Spectrum Analysis
chemical_list: D000179:Acrylates; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D003902:Detergents; D007840:Latex; D020366:Methylmethacrylate; C040833:ethyl acrylate; C036658:acrylic acid