Effect of ionizing radiation on rat tissue: proteomic and biochemical analysis.
Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.
Park
Eui-Chul
EC
Department of Biochemistry and Protein Network Research Center, Yonsei University, Seoul, Korea.
Yoon
Jong-Bok
JB
Seong
Jin-Sil
JS
Choi
Kyoung-Soo
KS
Kong
Eung-Sik
ES
Kim
Yun-Jeong
YJ
Park
Young-Mee
YM
Park
Eun-Mi
EM
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005720:Gamma Rays; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000528:radiation effects; D008099:Liver / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D011506:Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015854:Up-Regulation / Q000528:radiation effects
D011506:Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D005978:Glutathione