Quantitative determination of puerarin in dog plasma by HPLC and study on the relative bioavailability of sustained release tablets.
To evaluate the bioavailability of puerarin sustained release tablet (SR-Tab.) and Yufengningxin tablet (YU-Tab.), a liquid chromatography method was developed and validated to determine puerarin in dog plasma. Chromatographic separation was performed on Diamonsil C18 column using a mixture of methanol-acetic acid-water (25:6:69, v/v/v) delivered at a flow rate of 1.0 ml/min and detected by UV. 4-Hydroxybenzaldehyde was used as the internal standard. The linear range for puerarin was from 60 to 1800 ng/ml (r=0.9991) with a limit of quantitation of 60 ng/ml. Within-day accuracy and precision ranged from -3.0 to 2.2% and from 1.2 to 4.3%, between-day accuracy and precision ranged from -4.1 to 2.6% and from 1.3 to 5.7%, respectively. The mean extraction recoveries of puerarin determined over the three concentrations were (90.3+/-5.2)%, (95.7+/-1.4)% and (93.1+/-3.5)%. A significant difference was observed in main pharmacokinetic parameters of Tmax, Cmax and AUC0-infinity between puerarin SR-Tab. and YU-Tab. in dogs. The smoother plasma concentrations were obtained from SR-Tab. in dogs and the results were as expected.
Ren
Fuzheng
F
Department of Pharmaceutical Engineering, School of Chemistry and Pharmaceutics, East China University of Science and Technology, Shanghai 200237, China.
Jing
Qiufang
Q
Shen
Yongjia
Y
Ma
Hongmei
H
Cui
Jingbin
J
D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003692:Delayed-Action Preparations; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics; D007529:Isoflavones / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics
D003692:Delayed-Action Preparations; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D013607:Tablets; D014665:Vasodilator Agents; C033607:puerarin