title: Identification, cloning, and expression of the Scytalidium acidophilum XYL1 gene encoding for an acidophilic xylanase.
abstract: We cloned XYL1, a Scytalidium acidophilum gene encoding for an acidophilic family 11 xylanase. The XYL1p protein was expressed in Pichia pastoris using the pPICZalphaA expression plasmid. The secreted protein was purified by TAXI affinity column chromatography. The purified XYL1p showed an optimum activity at pH 3.2 and 56 degrees C. The Michaelis-Menten constants were determined.
authors.affiliation: Research Unit in Molecular Biology, University of Namur, Namur, Belgium.
authors.forename: Bassam
authors.initials: B
authors.lastname: Al Balaa
authors.forename: Johan
authors.initials: J
authors.lastname: Wouters
authors.forename: Sophie
authors.initials: S
authors.lastname: Dogne
authors.forename: Carlos
authors.initials: C
authors.lastname: Rossini
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Schaus
authors.forename: Eric
authors.initials: E
authors.lastname: Depiereux
authors.forename: Jean
authors.initials: J
authors.lastname: Vandenhaute
authors.forename: Isabelle
authors.initials: I
authors.lastname: Housen
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: D005656:Fungal Proteins