Mechanisms for inducing nasal mucosal tolerance in experimental autoimmune uveoretinitis.
Delivering soluble (auto) antigenic peptides via the naso-respiratory route induces tolerance to that peptide and suppression of experimental models of autoimmune disease. In the normal lung, respiratory tract dendritic cells (RTDCs) efficiently endocytose soluble antigens, migrate to regional lymph nodes and present peptide to T cells that subsequently become tolerant. This article describes protocols for inducing tolerance via the naso-respiratory tract in experimental autoimmune uveoretinitis (EAU); for the isolation of RTDCs to facilitate definition of, and conditions for, maturation and activation of cells; and to test RTDC ability to induce tolerance in murine EAU when adoptively transferred.
Calder
Claudia J
CJ
Department of Pathology and Microbiology, University of Bristol, UK.
Nicholson
Lindsay B
LB
Dick
Andrew D
AD
D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D001327:Autoimmune Diseases / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000637:transplantation; D005128:Eye Diseases / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D005136:Eye Proteins / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007108:Immune Tolerance / Q000276:immunology*; D018928:Immunity, Mucosal / Q000276:immunology; D007167:Immunotherapy / Q000379:methods*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009297:Nasal Mucosa / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology / Q000494:pharmacology; D012137:Respiratory System / Q000166:cytology / Q000276:immunology; D012173:Retinitis / Q000139:chemically induced / Q000473:pathology / Q000628:therapy; D012177:Retinol-Binding Proteins / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D014605:Uveitis / Q000139:chemically induced / Q000473:pathology / Q000628:therapy; D000099214:Retinol-Binding Proteins, Interstitial
D000954:Antigens, Surface; D016207:Cytokines; D005136:Eye Proteins; D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial