Multiplexed measurement of serum antibodies using an array biosensor.
The array biosensor provides the capability for simultaneously measuring titers of antibody against multiple antigens. Human antibodies against four different targets, tetanus toxin, diphtheria toxin, staphylococcal enterotoxin B (SEB) and hepatitis B, were measured simultaneously in sera from eight different donors in a single assay and titers were determined. The assays could measure amounts of bound antibody as low as approximately 100 fg. Each individual serum exhibited a different pattern of reactivity against the four target antigens. Applications of this biosensor capability include monitoring for exposure to pathogens and for efficacy of vaccination.
Moreno-Bondi
Maria C
MC
Department Química Analítica, Facultad de Química, Universidad Complutense, 28040 Madrid, Spain.
Taitt
Chris Rowe
CR
Shriver-Lake
Lisa C
LC
Ligler
Frances S
FS
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D015374:Biosensing Techniques; D004167:Diphtheria Toxin / Q000097:blood; D004768:Enterotoxins / Q000097:blood; D006041:Goats; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D040081:Protein Array Analysis; D013744:Tetanus Toxin / Q000097:blood
D000907:Antibodies, Bacterial; D004167:Diphtheria Toxin; D004768:Enterotoxins; D007074:Immunoglobulin G; D013744:Tetanus Toxin; C031818:enterotoxin B, staphylococcal