Modeled structure of the whole regulator G-protein signaling-2.
There is an increasing interest towards the mechanism by which regulators of G-protein signaling regulate signals of G-protein-coupled receptors. RGS2 is a regulator of Gq protein signaling (RGS), the N-terminal region of which is known to contain determinants for G protein-coupled receptor recognition, but its structure is still unknown. To understand the molecular basis for this recognition, the three-dimensional model of RGS2, including N-terminal region and RGS box, was modeled. For this, RGS4 box structure and data from circular dichroism study of RGS2 N-terminal region were used. Then, membrane-targeting activity of the RGS2 amphipathic helix contained in the N-terminal region was investigated. Furthermore, in cellulo study provided first evidence that an internal sequence within the N-terminal region of RGS2 is involved in RGS2 regulation of cholecystokinin receptor-2 signal. RGS2 modeled structure can now serve to study molecular recognition of RGS2 by signaling molecules.
Tikhonova
Irina G
IG
INSERM, Institut National de la Santé et de la Recherche Médicale, Unit 531, Institut Fédératif de recherche, Toulouse, France.
Boulègue
Cyril
C
Langer
Ingrid
I
Fourmy
Daniel
D
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D002942:Circular Dichroism; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D020710:RGS Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D020710:RGS Proteins