Biomarker discovery from the plasma proteome using multidimensional fractionation proteomics.
Because biomarkers are typically low in abundance, the crucial step of biomarker discovery is to efficiently separate clinically relevant sets of proteins that might define disease stages and/or predict disease development. It is anticipated that a multi-dimensional fractionation system (MDFS) will provide an efficient means of separating low abundance proteins from plasma proteins, resulting in the extension of the detection limit. However, when using an MDFS to analyze the plasma proteome it is important to consider how sample processing, yield, resolution and throughput potential may influence the detection limit. This review evaluates the recent advances in MDFS research with respect to '4RS criterion' (4R: resolution, reproducibility, recovery, and robustness; 4S: simplicity, speed, selectivity and sensitivity) and discusses perspectives for future plasma-derived biomarker discovery.
Lee
Hyoung-Joo
HJ
Department of Biochemistry, Yonsei Proteome Research Center, and Biomedical Proteome Research Center, Yonsei University, Seoul, South Korea, 120-749.
Lee
Eun-Young
EY
Kwon
Min-Seok
MS
Paik
Young-Ki
YK
D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity
D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome