title: Multiple antibiotic resistance among gram negative bacteria isolated from milk in Karachi.
abstract: A total of 100 Gram negative bacteria isolated from milk in Karachi were screened for their resistance to eleven commonly used antibiotics. The concentration of antibiotics used was 100 microg/ml. About 46% were found to resist antibiotics at the concentration of 100 microg/ml giving different patterns. The resistant bacteria were tested for the presence of R plasmids, both transferable and non-transferable, by conjugation, spontaneous segregation and curing with acridine orange and ethidium bromide. Among the resistant bacteria tested, eight were found to carry non-transferable R plasmids.
authors.affiliation: Department of Microbiology, University of Karachi, Karachi-75270, Pakistan.
authors.forename: A
authors.initials: A
authors.lastname: Ahmed
authors.forename: N
authors.initials: N
authors.lastname: Jameel
authors.forename: F A
authors.initials: FA
authors.lastname: Ansari
authors.forename: H
authors.initials: H
authors.lastname: Khatoon