title: A critical role for myosin IIb in dendritic spine morphology and synaptic function.
abstract: Dendritic spines show rapid motility and plastic morphology, which may mediate information storage in the brain. It is presently believed that polymerization/depolymerization of actin is the primary determinant of spine motility and morphogenesis. Here, we show that myosin IIB, a molecular motor that binds and contracts actin filaments, is essential for normal spine morphology and dynamics and represents a distinct biophysical pathway to control spine size and shape. Myosin IIB is enriched in the postsynaptic density (PSD) of neurons. Pharmacologic or genetic inhibition of myosin IIB alters protrusive motility of spines, destabilizes their classical mushroom-head morphology, and impairs excitatory synaptic transmission. Thus, the structure and function of spines is regulated by an actin-based motor in addition to the polymerization state of actin.
authors.affiliation: The Picower Institute for Learning and Memory, RIKEN-MIT Neuroscience Research Center, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors.forename: Jubin
authors.initials: J
authors.lastname: Ryu
authors.forename: Lidong
authors.initials: L
authors.lastname: Liu
authors.forename: Tak Pan
authors.initials: TP
authors.lastname: Wong
authors.forename: Dong Chuan
authors.initials: DC
authors.lastname: Wu
authors.forename: Alain
authors.initials: A
authors.lastname: Burette
authors.forename: Richard
authors.initials: R
authors.lastname: Weinberg
authors.forename: Yu Tian
authors.initials: YT
authors.lastname: Wang
authors.forename: Morgan
authors.initials: M
authors.lastname: Sheng
mesh_terms: D000818:Animals; D048429:Cell Size; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D003712:Dendrites / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure*; D004594:Electrophysiology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D024501:Nonmuscle Myosin Type IIB / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000502:physiology; D013569:Synapses / Q000378:metabolism / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D006576:Heterocyclic Compounds, 4 or More Rings; C472645:blebbistatin; D004247:DNA; D024501:Nonmuscle Myosin Type IIB