Reduction in channel catfish hepatic growth hormone receptor expression in response to food deprivation and exogenous cortisol.
The objective of this study was to assess the effects of food deprivation and exogenous cortisol administration on somatic growth of channel catfish, Ictalurus punctatus, and examine the resultant changes in circulating insulin-like growth factor-I (IGF-I) concentrations and growth hormone receptor (GHR) gene expression. Integral to this objective, we report the isolation, sequence, and characterization of channel catfish GHR. Sequence analysis and characterization results indicate sequence identity and tissue distribution similar to GHRs in other teleost fish and several functional characteristics conserved in known vertebrate GHRs. The effects of food deprivation and dietary exogenous cortisol administration were assessed as part of a 4-week study. Growth was significantly reduced after 4 weeks in cortisol-fed fish compared to fed-control fish, and fasting resulted in weight loss. At the end of the 4-week study, both IGF-I plasma concentrations and hepatic GHR mRNA abundance were significantly reduced in fasted and cortisol-fed catfish. Levels of hepatic GHR mRNA were positively correlated to circulating IGF-I levels. These results suggest that a reduction in hepatic GHR gene expression might serve as a mechanism for the reduction of circulating IGF-I and growth in channel catfish during periods of food deprivation and stress.
Small
Brian C
BC
USDA/ARS, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, P.O. Box 38, Stoneville, MS 38776, USA. bsmall@ars.usda.gov
Murdock
Christopher A
CA
Waldbieser
Geoffrey C
GC
Peterson
Brian C
BC
D000595:Amino Acid Sequence; D000818:Animals; D017756:Aquaculture; D001483:Base Sequence; D002397:Catfishes / Q000254:growth & development / Q000502:physiology*; D005508:Food Deprivation / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006854:Hydrocortisone / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D011986:Receptors, Somatotropin / Q000235:genetics* / Q000378:metabolism; D015431:Weight Loss / Q000187:drug effects / Q000502:physiology
D011986:Receptors, Somatotropin; D007334:Insulin-Like Growth Factor I; D006854:Hydrocortisone