Cockayne syndrome group B protein has novel strand annealing and exchange activities.
Cockayne syndrome (CS) is a rare inherited human genetic disorder characterized by UV sensitivity, severe neurological abnormalities and prageroid symptoms. The CS complementation group B (CSB) protein is involved in UV-induced transcription coupled repair (TCR), base excision repair and general transcription. CSB also has a DNA-dependent ATPase activity that may play a role in remodeling chromatin in vivo. This study reports the novel finding that CSB catalyzes the annealing of complementary single-stranded DNA (ssDNA) molecules with high efficiency, and has strand exchange activity. The rate of CSB-catalyzed annealing of complementary ssDNA is 25-fold faster than the rate of spontaneous ssDNA annealing under identical in vitro conditions and the reaction occurs with a high specificity in the presence of excess non-homologous ssDNA. The specificity and intrinsic nature of the reaction is also confirmed by the observation that it is stimulated by dephosphorylation of CSB, which occurs after UV-induced DNA damage, and is inhibited in the presence of ATPgammaS. Potential roles of CSB in cooperation with strand annealing and exchange activities for TCR and homologous recombination are discussed.
Muftuoglu
Meltem
M
Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, MD 21224, USA.
Sharma
Sudha
S
Thorslund
Tina
T
Stevnsner
Tinna
T
Soerensen
Martin M
MM
Brosh
Robert M
RM
Bohr
Vilhelm A
VA
D000255:Adenosine Triphosphate / Q000378:metabolism; D002384:Catalysis; D004265:DNA Helicases / Q000378:metabolism*; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000378:metabolism*; D010766:Phosphorylation; D011995:Recombination, Genetic; D051716:Replication Protein A / Q000378:metabolism
D004277:DNA, Single-Stranded; D051716:Replication Protein A; D000255:Adenosine Triphosphate; D004265:DNA Helicases