[Preparation of monoclonal antibodies to the Fc-fragment of human IgG and the use of their based immunoenzyme conjugates].
An original kit containing 17 clones of hybridomas, the producers of monoclonal antibodies (MCAb) against the Fc-fragments of human IgG, has been obtained. The possibility of using horseradish peroxidase conjugates of obtained MCAb as a part of ELISA test systems (Diaproph-Med, Ukraine) was comparatively studied. The application of peroxidase conjugates of two monoclonal antibodies (156C10 and 153H11) as part of five diagnostic kits was shown to provide a high specificity and a high sensitivity.
Nikolaenko
I V
IV
Galkin
A Iu
AIu
Raevskaia
G E
GE
Kas'ianenko
T V
TV
Nereshchenko
M I
MI
Donskaia
E S
ES
Spivak
N Ia
NIa
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007141:Immunoglobulin Fc Fragments / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic; D006735:Horseradish Peroxidase