title: Morphology and gelation of thermosensitive xyloglucan hydrogels.
abstract: Galactose modified xyloglucan is a thermally reversible hydrogel that is increasingly used in the biomedical field due to the ease of altering the gelation time and temperature by modifying the galactose removal ratio. However there is little information concerning the morphology and rheological properties of the hydrogel under physiological conditions. Differential scanning microcalorimetry (DSmicroC) showed the thermal gelation process to occur over a broad temperature range (5-50 degrees C). The rheological properties of the hydrogels were investigated as a function of concentration, temperature and ionic strength. The final elastic moduli of the hydrogels increased with increases in concentration. Isothermal rheology suggests that the gelation occurred in two distinct stages, which was influenced by the solution media. Scanning electron microscopy (SEM) was used to characterize the morphology of the xyloglucan which were thermally gelled at 37 degrees C. The resultant morphology was strongly dependent on the concentration of the hydrogel. Strong hydrogels were only obtained at 3 wt.% at 37 degrees C, and the morphology characterized by an open 3-dimensional network, comprised of thin membranes. It is proposed that the first stage of the isothermal gelation is the formation and growth of the thin membranes, followed by the formation of a three dimensional network.
authors.affiliation: Department of Materials Engineering, Monash University, Wellington Rd, Clayton, VIC 3800, Australia.
authors.forename: D R
authors.initials: DR
authors.lastname: Nisbet
authors.forename: K E
authors.initials: KE
authors.lastname: Crompton
authors.forename: S D
authors.initials: SD
authors.lastname: Hamilton
authors.forename: S
authors.initials: S
authors.lastname: Shirakawa
authors.forename: R J
authors.initials: RJ
authors.lastname: Prankerd
authors.forename: D I
authors.initials: DI
authors.lastname: Finkelstein
authors.forename: M K
authors.initials: MK
authors.lastname: Horne
authors.forename: J S
authors.initials: JS
authors.lastname: Forsythe
mesh_terms: D002152:Calorimetry, Differential Scanning; D005936:Glucans / Q000737:chemistry* / Q000648:ultrastructure; D020100:Hydrogels / Q000737:chemistry*; D015394:Molecular Structure; D012212:Rheology; D013696:Temperature; D014990:Xylans / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D005936:Glucans; D020100:Hydrogels; D014990:Xylans; C029353:xyloglucan