title: Betanodavirus induces phosphatidylserine exposure and loss of mitochondrial membrane potential in secondary necrotic cells, both of which are blocked by bongkrekic acid.
abstract: In this study, we show how the red spotted grouper nervous necrosis virus (RGNNV) causes loss of mitochondrial membrane potential and promotes host secondary apoptotic necrosis. RGNNV viral proteins such as protein alpha (42 kDa) and protein A (110 kDa) were quickly expressed between 12 h and 24 h postinfection (p.i.) in GL-av cells. Annexin V staining revealed that the NNV infection of GL-av cells induced phosphatidylserine (PS) externalization and development of bulb-like vesicles (bleb formation) at 24 h p.i. NNV infection also induced DNA fragmentation detectable by TUNEL assay between 12 h (8%) and 72 h (32%) p.i. Bongkrekic acid (1.6 microM; BKA) blocked permeability of the mitochondrial permeability transition pore, but cyclosporine A (CsA) did not block secondary necrosis. Finally, secondary necrotic cells were not engulfed by neighboring cells. Our data suggest that RGNNV induces apoptotic death via opening the mitochondrial permeability transition pore thereby triggering secondary necrosis in the mid-apoptotic phase.
authors.affiliation: Laboratory of Molecular Virology and Biotechnology, Institute of Biotechnology, National Cheng-Kung University, Tainan 701, Taiwan, ROC.
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Chen
authors.forename: Huey-Lang
authors.initials: HL
authors.lastname: Yang
authors.forename: Guor Mour
authors.initials: GM
authors.lastname: Her
authors.forename: Han-You
authors.initials: HY
authors.lastname: Lin
authors.forename: Mei-Fen
authors.initials: MF
authors.lastname: Jeng
authors.forename: Jen-Leih
authors.initials: JL
authors.lastname: Wu
authors.forename: Jiann-Ruey
authors.initials: JR
authors.lastname: Hong
mesh_terms: D017209:Apoptosis / Q000502:physiology; D001865:Bongkrekic Acid / Q000494:pharmacology*; D002460:Cell Line; D016572:Cyclosporine / Q000494:pharmacology; D005393:Fish Diseases / Q000821:virology*; D008564:Membrane Potentials / Q000187:drug effects* / Q000502:physiology; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D008930:Mitochondria, Liver / Q000187:drug effects; D009336:Necrosis; D030381:Nodaviridae / Q000472:pathogenicity*; D010718:Phosphatidylserines / Q000378:metabolism*
chemical_list: D010718:Phosphatidylserines; D001865:Bongkrekic Acid; D016572:Cyclosporine