title: Differing influence of sympathectomy on smooth muscle cells and fibroblasts in cerebral and peripheral muscular arteries.
abstract: In the present study, we examined the effect of sympathectomy on the distribution and the relative expression of cytoskeletal proteins used as markers of phenotypic modulation of vascular smooth muscle cells (SMCs) and myofibroblasts (MFBs) in rabbit femoral (FA) and basilar (BA) arteries. Adult rabbits were treated either with repeated 6-hydroxydopamine (6-OHDA) for sympathectomy or with vehicle for control. Cross sections taken from sympathectomized and control arteries 79 days later were immunolabelled for vimentin, desmin, alpha-smooth muscle actin (alpha-SM actin), beta-isoform of actin and h-caldesmon. The distribution of these proteins and the intensity of fluorescent labelled SMCs were examined under a confocal microscope. In the sympathectomized BA, there was no change for desmin, vimentin and h-caldesmon expression, but the expression of both alpha-SM actin and the beta-isoform was significantly higher (+19% and +30%, respectively). In the sympathectomized FA, the expression of the alpha- and beta-isoforms of actin remained unchanged, whereas those of desmin and vimentin were significantly higher (+35% and 17%, respectively) and h-caldesmon expression was lowered by 13%. In contrast to intact FAs, the external layers of sympathectomized FAs revealed migration of fibroblasts from the adventitia and death of SMCs. These results strongly suggest that sympathetic nerves intervene in the cytoskeletal protein remodelling through phenotypic modulation of both SMCs and MFBs during post-natal development, and in pathologies involving similar phenomena, such as atherosclerosis.
authors.affiliation: Laboratoire de Pathologies Vasculaires, Faculté des Sciences de Bizerte, 7021 Jarzouna, Bizerte, Tunisia. kamel.kacem@fsb.rnu.tn
authors.forename: K
authors.initials: K
authors.lastname: Kacem
authors.forename: R
authors.initials: R
authors.lastname: Sercombe
mesh_terms: D000818:Animals; D001158:Arteries / Q000166:cytology / Q000502:physiology; D001488:Basilar Artery / Q000166:cytology / Q000502:physiology; D002536:Cerebral Arteries / Q000166:cytology / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000098:blood supply / Q000166:cytology / Q000502:physiology*; D009130:Muscle, Smooth / Q000166:cytology / Q000502:physiology*; D016627:Oxidopamine; D010641:Phenotype; D011817:Rabbits; D012039:Regional Blood Flow / Q000502:physiology; D013563:Sympathectomy, Chemical; D013565:Sympatholytics
chemical_list: D009124:Muscle Proteins; D013565:Sympatholytics; D016627:Oxidopamine