Antiviral property and mechanisms of a sulphated polysaccharide from the brown alga Sargassum patens against Herpes simplex virus type 1.
A sulphated polysaccharide (SP-2a) from the brown alga Sargassum patens (Kütz.) Agardh (Sargassaceae) was found to significantly inhibit the in vitro replication of both the acyclovir (ACV)-sensitive and -resistant strains of Herpes simplex virus type 1 (HSV-1), in dose-dependent manners, with 50% inhibitions occurring with 1.5-5.3 microg/ml of the polysaccharide. SP-2a exhibited extracellular virucidal activity only against the ACV-sensitive strains, but not the resistant strain, at the concentration of 100 microg/ml. The strongest antiviral activities against the different strains of HSV-1 were observed when this polysaccharide was present during and after adsorption of the virus to host cells. The inhibitory effect of SP-2a on virus adsorption occurred dose-dependently in all the HSV-1 strains tested, and the adsorption of the ACV-resistant DM2.1 strain was reduced by 81.9% (relative to control) with 4 microg/ml of the polysaccharide. This study clearly demonstrated that the antiviral mode of action of SP-2a is mediated mainly by inhibiting virus attachment to host cells, and this sulphated polysaccharide might have different modes of action against the ACV-sensitive and -resistant strains of HSV-1.
Zhu
W
W
Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, and Laboratory of Lung Cancer Molecular Biology, Cancer Center, West China Hospital, Sichuan University, 37 Guoxue Lane, Sichuan 610041, China.
Chiu
L C M
LC
Ooi
V E C
VE
Chan
P K S
PK
Ang
P O
PO
D000212:Acyclovir / Q000494:pharmacology; D000327:Adsorption / Q000187:drug effects; D000998:Antiviral Agents / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D024882:Drug Resistance, Viral; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D011134:Polysaccharides / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D011134:Polysaccharides; D000212:Acyclovir