[Dynamic expression of hepatitis B virus covalently closed circular DNA in 2.2.15 cell].
BACKGROUND
To determine the presence of covalently closed circular DNA (cccDNA), and to investigate the expression kinetics of HBV DNA, HBsAg and HBeAg in 2.2.15 cell.


METHODS
HBV cccDNA was assessed by polymerase chain reaction, HBV DNA was measured by Taqman quantitative PCR and HBsAg and HBeAg was measured by EIA.


RESULTS
HBV cccDNA was found in both intracellular and extracellular space. There was a good correlation between HBsAg, HBeAg and HBV DNA in the supernatant of 2.2.15 cell (r= 0.833, P < 0.05 and r= 0.939, P < 0.01 for HBsAg and HBeAg, respectively), whereas there was no significant correlation between intracellular HBV DNA levels and virus antigen levels (r= 0.024, P= 0.955 and r= 0.177; P= 0.625 for HBsAg and HBeAg, respectively).


CONCLUSIONS
HBV cccDNA was detectable in the culture medium and intracellularly in 2.2.15 cells, and these data provided an indication of HBV replication in 2.2.15 cell.
Liu
Mao-chang
MC
Department of Infectious Diseases, Peking University First Hospital, Beijing 100034, China.
Wang
Gui-qiang
GQ
Piao
Wen-hua
WH
Xi
Hong-li
HL
Lu
Hai-ying
HY
Wang
Yan
Y
Wang
Qin-huan
QH
D045744:Cell Line, Tumor; D004270:DNA, Circular / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006513:Hepatitis B e Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
D004270:DNA, Circular; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens