Concentrations of gastric mucosal cytokines in children with food allergy and Helicobacter pylori infection.
OBJECTIVE
To measure the concentrations of chosen cytokines in the antrum mucosa depending on the kind of harmful pathogenic factors and to compare the concentrations with the values of controls without allergy and coexisting Helicobacter pylori (H pylori) infection.


METHODS
The patients (97 children) were divided into three groups according to the data obtained from the case history, to the main cause of the disease and to the dominant clinical symptoms. Group I: children with food allergy (Fa); group II: children infected with H pylori; group III (control group): children with functional disorders of the alimentary tract (without Fa and Hp infection). H pylori infection was determined by the presence of anti-Hp antibodies in serum (ELISA method) and urease test performed during endoscopic examination. Cytokine concentration in homogenates of gastric mucosa was detected by ELISA method.


RESULTS
The IL-2 concentration in gastric mucosa bioptates was the highest in children with Hp infection (116.5+/-179.5 pg/mg of the protein) and Fa and Hp infection (98.1+/-101.0 pg/mg), while decreased in children with Fa (44.8+/-50.3 pg/mg) and controls (45.7+/-23.5 pg/mg). The lowest mean concentration of IFN-gamma was observed in children with Hp infection (18.9+/-16.4 pg/mg), with Fa and Hp infection (25.5+/-27.7 pg/mg), with Fa (40.6+/-39.7 pg/mg) and controls (49.9+/-33.4 pg/mg). The highest IL-4 concentrations were observed in children with Hp infection (35.3+/-52.8 pg/mg) and in children with Fa and Hp infection (37.2+/-51.7 pg/mg), while lower IL-4 concentration (23.6+/-35.8 pg/mg) was found in children with Fa compared to the controls (22.7+/-13.8 pg/mg). The analysis of IL-4 concentrations in children with Hp infection regarding the intensity of gastritis showed the highest value (62.2+/-61.2 pg/mg) in mild and moderate gastritis. The concentrations of IL-5 in the gastric mucosa of children with or without Fa did not differ significantly and were comparable to the control group. The highest mean IL-8 value was observed in Hp-infected children with or without Fa. The highest concentration of mucosal IL-10 was detected in children with Hp infection (79.3+/-41.2 pg/mg) and decreased in children with Fa and Hp infection (50.1+/-18.8 pg/mg) and in children with Fa (39.9+/-35.5 pg/mg). The intensity and activity of the inflammation did not affect IL-10 concentrations in the gastric mucosa. In children with Hp infection, TNF-alpha concentration was the highest (45.9+/-49.3 pg/mg) and in children with Fa and Hp infection was low (45.3+/-32.6 pg/mg), whereas decreased in children with Fa (21.7+/-34.2 pg/mg) and in controls (31.6+/-14.5 pg/mg).


CONCLUSIONS
The morphological changes of the gastric mucosa in children with Hp infection are comparable to those in children with Fa and coexisting Hp infection. Cytokine concentration in children with Fa and Hp infection is significantly different in IFN-gamma, IL-2, IL-8, and TNF-alpha.
Maciorkowska
Elzbieta
E
Department of Pediatric Nursing, Medical University of Bialystok, Waszyngtona Str. 15, 15-274 Bialystok, Poland. emaciorkowska@o2.pl
Panasiuk
Anatol
A
Kaczmarsk
Maciej
M
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000276:immunology*; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D005753:Gastric Mucosa / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D005756:Gastritis / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008297:Male; D011706:Pyloric Antrum / Q000276:immunology / Q000382:microbiology / Q000473:pathology
D016207:Cytokines