title: Porphyromonas gingivalis heat shock protein vaccine reduces the alveolar bone loss induced by multiple periodontopathogenic bacteria.
abstract: OBJECTIVE
  Heat shock protein (HSP) can be utilized as a vaccine to cross-protect against multiple pathogenic species. The present study was performed to evaluate Porphyromonas gingivalis heat shock protein 60 (HSP60) as a vaccine candidate to inhibit multiple bacteria-induced alveolar bone loss.
  
  
  METHODS
  Recombinant P. gingivalis HSP60 was produced and purified from P. gingivalis GroEL gene. Rats were immunized with P. gingivalis HSP60, and experimental alveolar bone loss was induced by infection with multiple periodontopathogenic bacteria.
  
  
  RESULTS
  There was a very strong inverse relationship between postimmune anti-P. gingivalis HSP immunoglobulin G (IgG) levels and the amount of alveolar bone loss induced by either P. gingivalis or multiple bacterial infection (p=0.007). Polymerase chain reaction data indicated that the vaccine successfully eradicated the multiple pathogenic species.
  
  
  CONCLUSIONS
  We concluded that P. gingivalis HSP60 could potentially be developed as a vaccine to inhibit periodontal disease induced by multiple pathogenic bacteria.
authors.affiliation: Department of Periodontology, School of Dentistry and Research Institute for Oral Biotechnology Pusan National University, Pusan, Korea.
authors.forename: Ju-Youn
authors.initials: JY
authors.lastname: Lee
authors.forename: Ni-Na
authors.initials: NN
authors.lastname: Yi
authors.forename: Ueon-Suk
authors.initials: US
authors.lastname: Kim
authors.forename: Jeoung-Soon
authors.initials: JS
authors.lastname: Choi
authors.forename: Sung-Jo
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jeom-Il
authors.initials: JI
authors.lastname: Choi
mesh_terms: D000189:Actinobacillus Infections / Q000517:prevention & control; D016976:Aggregatibacter actinomycetemcomitans; D016301:Alveolar Bone Loss / Q000382:microbiology / Q000517:prevention & control*; D000818:Animals; D001428:Bacterial Vaccines; D016866:Bacteroidaceae Infections / Q000517:prevention & control; D001442:Bacteroides Infections / Q000517:prevention & control; D018834:Chaperonin 60 / Q000276:immunology / Q000627:therapeutic use*; D005674:Fusobacterium Infections / Q000517:prevention & control; D016967:Fusobacterium nucleatum; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000517:prevention & control*; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D016966:Porphyromonas gingivalis / Q000276:immunology*; D018713:Prevotella intermedia; D051381:Rats; D017207:Rats, Sprague-Dawley; D048373:Treponema denticola; D014211:Treponemal Infections / Q000517:prevention & control
chemical_list: D001428:Bacterial Vaccines; D018834:Chaperonin 60; D007074:Immunoglobulin G