Self-association and lipid binding properties of the lipoprotein initiating domain of apolipoprotein B.
The amino-terminal 20.1% of apolipoprotein B (apoB20.1; residues 1-912) is sufficient to initiate and direct the formation of nascent apoB-containing lipoprotein particles. To investigate the mechanism of initial lipid acquisition by apoB, we examined the lipid binding and interfacial properties of a carboxyl-terminal His6-tagged form of apoB20.1 (apoB20.1H). ApoB20.1H was expressed in Sf9 cells and purified by nickel affinity chromatography. ApoB20.1H was produced in a folded state as characterized by formation of intramolecular disulfide bonds and resistance to chemical reduction. Dynamic light scattering in physiological buffer indicated that purified apoB20.1H formed multimers, which were readily dissociable upon the addition of nonionic detergent (0.1% Triton X-100). ApoB20.1H was incapable of binding dimyristoylphosphatidylcholine multilamellar vesicles, unless its multimeric structure was first disrupted by guanidine hydrochloride. However, apoB20.1H multimers spontaneously dissociated and bound to the interface of naked and phospholipid-coated triolein droplets. These data reveal that the initiating domain of apoB contains solvent-accessible hydrophobic sequences, which, in the absence of a hydrophobic lipid interface or detergent, engage in self-association. The high affinity of apoB20.1H for neutral lipid is consistent with the membrane binding and desorption model of apoB-containing lipoprotein assembly.
Ledford
Aubrey S
AS
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157-1040, USA.
Weinberg
Richard B
RB
Cook
Victoria R
VR
Hantgan
Roy R
RR
Shelness
Gregory S
GS
D000596:Amino Acids; D000818:Animals; D001055:Apolipoproteins B / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001665:Binding Sites; D002021:Buffers; D003902:Detergents / Q000494:pharmacology; D004134:Dimyristoylphosphatidylcholine; D004220:Disulfides / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D019791:Guanidine / Q000494:pharmacology; D006639:Histidine / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008027:Light; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D013058:Mass Spectrometry; D008970:Molecular Weight; D017830:Octoxynol / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012542:Scattering, Radiation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018411:Spodoptera / Q000166:cytology / Q000378:metabolism; D014162:Transfection; D014304:Triolein / Q000378:metabolism
D000596:Amino Acids; D001055:Apolipoproteins B; D002021:Buffers; D003902:Detergents; D004220:Disulfides; D008074:Lipoproteins; D008081:Liposomes; D011994:Recombinant Proteins; D014304:Triolein; D006639:Histidine; D017830:Octoxynol; D019791:Guanidine; D004134:Dimyristoylphosphatidylcholine