A new lignan from the Jian-er syrup and its content determination by RP-HPLC.
The Jian-er syrup is a commonly used traditional Chinese medicinal preparation refined from two herbs, Metaplexis japonica (Thunb.) Makino and Justicia procumbens L. A new lignan, named 6'-hydroxy-Justicidin B, was isolated from the ethyl acetate extract of the Jian-er syrup and its structure was established on the basis of spectral analysis. The content of 6'-hydroxy-Justicidin B in the Jian-er syrup was determined by RP-HPLC for the first time. Chromatographic separation was achieved on an Agilent Zorbax SB-C18 column (150 mmx4.6 mm i.d., 5 microm) at 25 degrees C and the mobile phase was a mixture of acetonitrile and water (31:69 v/v) detected at 256 nm at a flow rate of 1.0 ml/min. This method was validated in terms of selectivity, linearity, precision, accuracy, limit of detection, limit of quantitation and solution stability. It can be used to control the quality of this preparation effectively.
Yang
Meihua
M
Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100094, PR China. yangmeihua15@hotmail.com
Wu
Jun
J
Xu
Xudong
X
Jin
Yue
Y
Guo
Yi
Y
Chen
Jianmin
J
D029062:Apocynaceae; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004148:Dioxolanes / Q000032:analysis* / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D017705:Lignans / Q000032:analysis* / Q000302:isolation & purification; D015394:Molecular Structure; D010946:Plants, Medicinal; D015203:Reproducibility of Results
D004148:Dioxolanes; D004365:Drugs, Chinese Herbal; C521474:Jian-er; D017705:Lignans; C049719:justicidins