Sulforaphane inhibits osteoclastogenesis by inhibiting nuclear factor-kappaB.
We show that sulforaphane inhibits osteoclastogenesis in the presence of macrophage colony-stimulating factor (M-CSF) and receptor for activation of nuclear factor-kB ligand (RANKL) in osteoclast (OC) precursors. Sulforaphane, an aliphatic isothiocyanate, is a known cancer chemo-preventative agent with anti-oxidative properties. Nuclear factor-kB (NF-kB) is a critical transcription factor in RANKL-induced osteoclastogenesis, and electrophoretic mobility shift assays (EMSAs) and assay of NF-kB-mediated secreted alkaline phosphatase (SEAP) revealed that sulforaphane selectively inhibited NF-kappaB activation induced by RANKL. Inhibition may involve interaction of sulforaphane with thiol groups, since it was prevented by reducing agents.
Kim
Soo-Jin
SJ
Department of Biological Sciences, and Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Korea.
Kang
So-Young
SY
Shin
Hyun-Hee
HH
Choi
Hye-Seon
HS
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D002352:Carrier Proteins / Q000494:pharmacology; D002478:Cells, Cultured; D024202:Electrophoretic Mobility Shift Assay; D017879:Isothiocyanates; D008562:Membrane Glycoproteins / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D010010:Osteoclasts / Q000187:drug effects* / Q000378:metabolism; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013454:Sulfoxides; D013861:Thiocyanates / Q000494:pharmacology*
D002352:Carrier Proteins; D017879:Isothiocyanates; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D013438:Sulfhydryl Compounds; D013454:Sulfoxides; D013861:Thiocyanates; C505687:Tnfrsf11a protein, mouse; C505689:Tnfsf11 protein, mouse; D000469:Alkaline Phosphatase; C016766:sulforaphane