title: Short-term bioaccumulation of vanadium when ingested with a tea decoction in streptozotocin-induced diabetic rats.
abstract: Sodium orthovanadate suspended in a lichee black tea decoction effectively regulates blood glucose levels in rats with insulin-dependent, streptozotocin (STZ)-induced diabetes. The primary advantage of vanadate delivery with the tea decoction over conventional systems that use water suspensions of vanadate is a significant reduction in the toxic side effects of vanadate. It is unknown if the tea alters the bioavailability of vanadate. Male Sprague-Dawley rats were administered an intravenous injection of STZ to induce diabetes. Four days later, the diabetic rats were treated by oral gavage with 40 mg of Na-orthovanadate suspended in double-distilled, deionized water (V/H2O), tea/vanadate (TV) decoction, or were treated with the tea decoction alone. Vanadium concentrations were measured in blood and various tissues at 1 to 24 hours posttreatment using graphite furnace atomic absorption spectrophotometry. With the exception of bone, maximal vanadium concentration in plasma and tissue samples were observed 2 hours after ingestion, but steadily decreased after that. Plasma vanadium levels continued to decrease until 16 hours. In contrast, vanadium steadily accumulated in bone over the 24-hour period. Overall, rats treated with V/H2O contained similar or significantly higher concentrations of vanadium in all tissues compared with TV treatment. The pattern of vanadium accumulation was also similar over time in both treatment groups. Vanadium levels were highest in bone > kidney > liver > pancreas > lung > heart > muscle > brain in both TV- and V/H2O-treated animals. This study demonstrates that the accumulation of vanadium in diabetic rats is reduced when coadministered with a black tea decoction in comparison to administration of vanadium in water. However, this effect is unlikely to be of a magnitude to explain the full capacity of TV to reduce the toxic side effects of vanadate.
authors.affiliation: National Centre for Agri-food Research in Medicine, St Boniface General Hospital Research Centre, Winnipeg, Manitoba, Canada R2H 2A6.
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Edel
authors.forename: Melanie
authors.initials: M
authors.lastname: Kopilas
authors.forename: Tod A
authors.initials: TA
authors.lastname: Clark
authors.forename: Floribeth
authors.initials: F
authors.lastname: Aguilar
authors.forename: Pallub K
authors.initials: PK
authors.lastname: Ganguly
authors.forename: Clayton E
authors.initials: CE
authors.lastname: Heyliger
authors.forename: Grant N
authors.initials: GN
authors.lastname: Pierce
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013054:Spectrophotometry, Atomic; D013662:Tea; D014018:Tissue Distribution; D014638:Vanadates / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology
chemical_list: D001786:Blood Glucose; D013662:Tea; D014638:Vanadates