Epidermal growth factor affects the synthesis and degradation of type I collagen in cultured human dermal fibroblasts.
EGF and type I collagen are known to play important roles in wound healing. In the present study, we demonstrated that EGF down-regulates the expression of type I procollagen protein as well as alpha2(I) collagen mRNA in cultured human dermal fibroblasts. EGF induced the degradation of type I procollagen protein in conditioned medium through the up-regulation of MMP-1 expression. EGF down-regulated alpha2(I) mRNA expression partially at the post-transcriptional level by reducing the mRNA stability. In contrast, EGF up-regulated MMP-1 mRNA expression mostly at the transcriptional level, in that it had a stimulatory effect on MMP-1 promoter activity, but no effect on MMP-1 mRNA stability. The MEK/ERK signaling pathway was shown to be involved in EGF-mediated type I collagen and MMP-1 expression.
Mimura
Yoshihiro
Y
Department of Dermatology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Ihn
Hironobu
H
Jinnin
Masatoshi
M
Asano
Yoshihide
Y
Yamane
Kenichi
K
Tamaki
Kunihiko
K
D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D020405:Dermis / Q000166:cytology*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D020935:MAP Kinase Signaling System; D011994:Recombinant Proteins / Q000494:pharmacology; D014945:Wound Healing
D024042:Collagen Type I; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor