[Building immune microsphere against tumor necrosis factor-alpha (TNF-alpha)].
We have constructed the immune microsphere against tumor necrosis factor-alpha (TNF-alpha) prospectively, hoping to establish the experiment groundwork in more researches which could be used in specific elimination of the TNF-alpha by blood purification method for the future. The recombinant human tumor necrosis factor-alpha monoclonal antibody (rHTNF-alpha McAb) was wrapped on the polystyrene microsphere (PSM) carrier connecting poly-L-lysine (PLL) beforehand. They were earmarked by the fluorescein isothiocyanate (FITC) respectively. The packing conditions were examined using the inversted and fluorescence microscopes and the spectrophotometer. The results showed that the best conditions for wrapping were 20 degrees C, pH9.5 and 60 minutes. The PLL content was not changed in the washing fluid after coating, which indicated the wrapping was quite firm. At the same temperature and same coating time, the rHTNF-alpha McAb coated on the PLL was obviously substantial when the concentration of glutaraldehyde solution was 0.2%. The findings demonstrated that the built immune microsphers can be used as a novel adsorption material. This method is simple and economic, and it offers a new approach to the related studies.
Wang
Qin
Q
Division of Kidney, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
Wu
Xiongfei
X
Wang
Junxia
J
Liu
Hong
H
Li
Lian
L
Jin
Xiyu
X
D000911:Antibodies, Monoclonal / Q000276:immunology*; D019793:Fluorescein / Q000737:chemistry; D006801:Humans; D008863:Microspheres; D011107:Polylysine / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D011994:Recombinant Proteins / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011137:Polystyrenes; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D011107:Polylysine; D019793:Fluorescein