Comparison of Campylobacter isolated from humans and food-producing animals in Japan.
OBJECTIVE
To compare the antimicrobial resistance, serotypes and flagellin gene types of Campylobacter isolated from humans and food-producing animals and thereby facilitate elucidation of the origin of Campylobacter causing human infection in Japan.


RESULTS
The MIC values of ampicillin, dihydrostreptomycin, gentamicin, erythromycin, oxytetracycline, nalidixic acid and enrofloxacin for Campylobacter isolated from humans (134 isolates), cattle (38 isolates), pigs (69 isolates), layers (84 isolates) and broilers (51 isolates) were compared. The MIC(90) values of ampicillin for Campylobacter jejuni isolates from poultry were higher than those from humans and cattle. Campylobacter coli that was resistant to dihydrostreptomycin and erythromycin was observed at a higher frequency in humans and pigs than in poultry. The restriction fragment profiles of flaA of human, bovine and broiler isolates were analysed by clustering, and the isolates were classified into five clusters. Cluster I contained only human and bovine isolates. Clusters III, IV and V contained human, bovine and broiler isolates.


CONCLUSIONS
Campylobacter isolates from humans included isolates that exhibited characteristics identical to those of the bovine, porcine and poultry isolates.


CONCLUSIONS
In addition to poultry, cattle and pigs are believed to be sources of campylobacteriosis in Japan.
Ishihara
K
K
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Japan. kanako_ishihara@nm.maff.go.jp
Yamamoto
T
T
Satake
S
S
Takayama
S
S
Kubota
S
S
Negishi
H
H
Kojima
A
A
Asai
T
T
Sawada
T
T
Takahashi
T
T
Tamura
Y
Y
D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D002167:Campylobacter / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D017000:Campylobacter coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D016000:Cluster Analysis; D004096:Dihydrostreptomycin Sulfate / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D000077422:Enrofloxacin; D004917:Erythromycin / Q000494:pharmacology; D005408:Flagellin / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology; D005516:Food Microbiology; D005798:Genes, Bacterial / Q000235:genetics; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009268:Nalidixic Acid / Q000494:pharmacology; D010118:Oxytetracycline / Q000494:pharmacology; D011200:Poultry / Q000382:microbiology; D012703:Serotyping / Q000379:methods; D013552:Swine / Q000382:microbiology
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D005839:Gentamicins; D005408:Flagellin; D009268:Nalidixic Acid; D000077422:Enrofloxacin; D004917:Erythromycin; D000667:Ampicillin; D004096:Dihydrostreptomycin Sulfate; D010118:Oxytetracycline