title: Effect of ionizing radiation on rat tissue: proteomic and biochemical analysis.
abstract: Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.
authors.affiliation: Department of Biochemistry and Protein Network Research Center, Yonsei University, Seoul, Korea.
authors.forename: Eui-Chul
authors.initials: EC
authors.lastname: Park
authors.forename: Jong-Bok
authors.initials: JB
authors.lastname: Yoon
authors.forename: Jin-Sil
authors.initials: JS
authors.lastname: Seong
authors.forename: Kyoung-Soo
authors.initials: KS
authors.lastname: Choi
authors.forename: Eung-Sik
authors.initials: ES
authors.lastname: Kong
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Kim
authors.forename: Young-Mee
authors.initials: YM
authors.lastname: Park
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Park
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005720:Gamma Rays; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000528:radiation effects; D008099:Liver / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D011506:Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015854:Up-Regulation / Q000528:radiation effects
chemical_list: D011506:Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D005978:Glutathione