Analysis of protein redox modification by hypoxia.
We examined hypoxia-induced changes in global thiol proteome profile in human prostate cancer cells using a BIAM-based display method. We analyzed the kinetics of protein thiol modification by using a pattern recognition algorithm, self-organizing maps (SOM) clustering, and identified the BIAM-labeled proteins by MALDI-TOF and ESI-tandem mass spectrometry. We found 99 out of 215 of total BIAM-labeled proteins were affected by hypoxia treatment and, yet, with diverse patterns and kinetics of redox modification. Our study proved that proteomics analysis employing the BIAM-labeling method can provide valuable information pertaining to global changes in the redox status of proteins in response to hypoxia.
Choi
Kyoung-Soo
KS
Department of Cellular Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Park
Soo-Yeon
SY
Baek
Sun-Hee
SH
Dey-Rao
Rama
R
Park
Young-Mee
YM
Zhang
Haitao
H
Ip
Clement
C
Park
Eun-Mi
EM
Kim
Yeul Hong
YH
Park
Jong Hoon
JH
D017209:Apoptosis / Q000502:physiology; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D020169:Caspases / Q000378:metabolism; D015687:Cell Hypoxia / Q000502:physiology; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005029:Ethylenediamines / Q000737:chemistry; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D010084:Oxidation-Reduction; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005029:Ethylenediamines; C093128:N-biotinoyl-N'-iodoacetylethylenediamine; D011506:Proteins; D020543:Proteome; D017382:Reactive Oxygen Species; D001710:Biotin; D011065:Poly(ADP-ribose) Polymerases; D048031:JNK Mitogen-Activated Protein Kinases; D020169:Caspases