title: Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model.
abstract: To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90% of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with naïve D3-derived cells ( approximately 28% DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.
authors.affiliation: Brain Korea 21 Project for Medical Science and Department of Neurosurgery, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Cho
authors.forename: Dae-Sung
authors.initials: DS
authors.lastname: Kim
authors.forename: Peter Geon
authors.initials: PG
authors.lastname: Kim
authors.forename: Yong Sup
authors.initials: YS
authors.lastname: Hwang
authors.forename: Myung Soo
authors.initials: MS
authors.lastname: Cho
authors.forename: Shin Yong
authors.initials: SY
authors.lastname: Moon
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Kim
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Chang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism*; D008297:Male; D001523:Mental Disorders / Q000175:diagnosis / Q000517:prevention & control*; D009474:Neurons / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation*; D020734:Parkinsonian Disorders / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology*; D016896:Treatment Outcome
chemical_list: D004298:Dopamine