Comparison of the SYBR Green and the hybridization probe format for real-time PCR detection of HHV-6.
A comparative study was conducted of a novel real-time quantitative PCR test (LightCycler System) with FastStart DNA Master(PLUS) SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence resonance energy transfer method, and with those of a single qualitative PCR test. The detection limit of the test with SYBR Green I dye was 20 copies of the virus, similar to that of the other two tests. The reproducibility was satisfactory. The new test has the same advantages as real-time PCR with HP formats and offers a greater versatility at lower cost.
Fernández
F
F
Department of Microbiology, Service of Immunology, University of Granada, Virgen de las Nieves University Hospital, Granada, Spain.
Gutiérrez
J
J
Sorlózano
A
A
Romero
J M
JM
Soto
M J
MJ
Ruiz-Cabello
F
F
D052160:Benzothiazoles; D015342:DNA Probes; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D003959:Diamines; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009930:Organic Chemicals / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines; D019349:Roseolovirus Infections / Q000821:virology*
D052160:Benzothiazoles; D015342:DNA Probes; D004279:DNA, Viral; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I