Partial feed restriction decreases growth hormone receptor 1A mRNA expression in postpartum dairy cows.
Uncoupling of the growth hormone (GH) axis in early postpartum dairy cows is correlated with a decrease in liver GH receptor (GHR) 1A mRNA and a decrease in liver GH receptor protein. Postpartum recoupling of the GH axis is also correlated with GHR 1A mRNA and GHR protein. We hypothesized that dry matter intake (DMI) partially controls the increase in GHR 1A mRNA postpartum. Prepartum Holstein dairy cows (n = 11) were offered feed ad libitum. After calving, 6 cows were fed 70% of their expected DMI (feed restriction) for 14 d and 5 cows were fed ad libitum (control). Both groups were fed ad libitum after d 14. Liver was biopsied prepartum and on d 1, 7, 14, and 21 postpartum; blood was sampled throughout the experimental period. Rate of increase in postpartum milk production was less for feed-restricted cows. The GHR 1A mRNA decreased from prepartum to d 1 postpartum and subsequently increased. Rate of postpartum increase in GHR 1A mRNA was less in feed-restricted cows. Diminished GHR 1A persisted for at least 7 d after feed-restricted cows returned to ad libitum feeding. Liver insulin-like growth factor-I mRNA concentrations decreased from prepartum to d 1 as well, but were similar for feed restricted and control thereafter. We concluded that DMI partially controls GHR 1A mRNA expression in early postpartum dairy cows and that the decrease in GHR 1A in response to feed restriction persisted for at least 1 wk after ad libitum feeding was restored.
Radcliff
R P
RP
Division of Animal Sciences, University of Missouri, Columbia 65211, USA.
McCormack
B L
BL
Keisler
D H
DH
Crooker
B A
BA
Lucy
M C
MC
D020155:3-Hydroxybutyric Acid / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D002417:Cattle / Q000378:metabolism*; D021983:Cyclophilins / Q000235:genetics; D004032:Diet; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005508:Food Deprivation; D013006:Growth Hormone / Q000097:blood; D007328:Insulin / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000235:genetics; D007774:Lactation; D008099:Liver / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D049590:Postpartum Period; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis*; D011986:Receptors, Somatotropin / Q000235:genetics*
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D012333:RNA, Messenger; D011986:Receptors, Somatotropin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D021983:Cyclophilins; D020155:3-Hydroxybutyric Acid