Amelogenin-mediated regulation of osteoclastogenesis, and periodontal cell proliferation and migration.
We previously reported that amelogenin isoforms M180 and leucine-rich amelogenin peptide (LRAP) are expressed in the periodontal region, and that their absence is associated with increased cementum defects in amelogenin-knockout (KO) mice. The aim of the present study was to characterize the functions of these isoforms in osteoclastogenesis and in the proliferation and migration of cementoblast/periodontal ligament cells. The co-cultures of wild-type (WT) osteoclast progenitor and KO cementoblast/periodontal ligament cells displayed more tartrate-resistant acid phosphatase (TRAP)-positive cells than the co-cultures of WT cells. The addition of LRAP to both co-cultures significantly reduced RANKL expression and the TRAP-positive cells. Proliferation and migration rates of the KO cementoblast/periodontal ligament cells were lower than those of WT cells and increased with the addition of either LRAP or P172 (a porcine homolog of mouse M180). Thus, we demonstrate the regulation of osteoclastogenesis by LRAP, and the proliferation and migration of cementoblast/periodontal ligament cells by LRAP and P172.
Hatakeyama
J
J
Functional Genomics Section, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD 20892, USA.
Philp
D
D
Hatakeyama
Y
Y
Haruyama
N
N
Shum
L
L
Aragon
M A
MA
Yuan
Z
Z
Gibson
C W
CW
Sreenath
T
T
Kleinman
H K
HK
Kulkarni
A B
AB
D053523:Amelogenin; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D003739:Dental Cementum / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D003746:Dental Enamel Proteins / Q000494:pharmacology / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D018345:Mice, Knockout; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D020033:Protein Isoforms / Q000494:pharmacology / Q000502:physiology; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D011994:Recombinant Proteins / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D013552:Swine
D053523:Amelogenin; C507200:Amelx protein, mouse; D002352:Carrier Proteins; D003746:Dental Enamel Proteins; D008562:Membrane Glycoproteins; D020033:Protein Isoforms; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D011994:Recombinant Proteins; C505687:Tnfrsf11a protein, mouse; C505689:Tnfsf11 protein, mouse; C067432:leucine-rich amelogenin peptide