Dopamine neurons derived from embryonic stem cells efficiently induce behavioral recovery in a Parkinsonian rat model.
To test the in vivo effect of a high yield of dopaminergic (DA) neurons (90% of total neurons) which had been generated from a genetically modified mouse embryonic stem cell line, N2, the cells were transplanted into a rat model of Parkinson's disease (PD). The PD animals grafted with N2-derived cells showed significant behavior improvements compared with sham controls from 2 weeks posttransplantation, whereas animals with naïve D3-derived cells ( approximately 28% DA neurons of total neurons) showed only a modest recovery. Furthermore, hyperactivity observed in the subthalamic nucleus, pedunculopontine nucleus, and substantia nigra pars reticulata of PD rat models was dramatically reduced by the grafting of N2-derived cells. The number of DA neurons in the striatum which originated from N2 grafting was much higher compared to that from D3 grafting, and the neurons efficiently released DA in the brain, showing a good correlation with behavioral recovery.
Cho
Yoon Hee
YH
Brain Korea 21 Project for Medical Science and Department of Neurosurgery, Yonsei University College of Medicine, Seoul, Republic of Korea.
Kim
Dae-Sung
DS
Kim
Peter Geon
PG
Hwang
Yong Sup
YS
Cho
Myung Soo
MS
Moon
Shin Yong
SY
Kim
Dong-Wook
DW
Chang
Jin Woo
JW
D000818:Animals; D002454:Cell Differentiation; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism*; D008297:Male; D001523:Mental Disorders / Q000175:diagnosis / Q000517:prevention & control*; D009474:Neurons / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation*; D020734:Parkinsonian Disorders / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology*; D016896:Treatment Outcome
D004298:Dopamine