title: [Dynamic expression of hepatitis B virus covalently closed circular DNA in 2.2.15 cell].
abstract: BACKGROUND
  To determine the presence of covalently closed circular DNA (cccDNA), and to investigate the expression kinetics of HBV DNA, HBsAg and HBeAg in 2.2.15 cell.
  
  
  METHODS
  HBV cccDNA was assessed by polymerase chain reaction, HBV DNA was measured by Taqman quantitative PCR and HBsAg and HBeAg was measured by EIA.
  
  
  RESULTS
  HBV cccDNA was found in both intracellular and extracellular space. There was a good correlation between HBsAg, HBeAg and HBV DNA in the supernatant of 2.2.15 cell (r= 0.833, P < 0.05 and r= 0.939, P < 0.01 for HBsAg and HBeAg, respectively), whereas there was no significant correlation between intracellular HBV DNA levels and virus antigen levels (r= 0.024, P= 0.955 and r= 0.177; P= 0.625 for HBsAg and HBeAg, respectively).
  
  
  CONCLUSIONS
  HBV cccDNA was detectable in the culture medium and intracellularly in 2.2.15 cells, and these data provided an indication of HBV replication in 2.2.15 cell.
authors.affiliation: Department of Infectious Diseases, Peking University First Hospital, Beijing 100034, China.
authors.forename: Mao-chang
authors.initials: MC
authors.lastname: Liu
authors.forename: Gui-qiang
authors.initials: GQ
authors.lastname: Wang
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Piao
authors.forename: Hong-li
authors.initials: HL
authors.lastname: Xi
authors.forename: Hai-ying
authors.initials: HY
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Qin-huan
authors.initials: QH
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor; D004270:DNA, Circular / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006513:Hepatitis B e Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens