title: Gene expression profiles in acute myeloid leukemia with common translocations using SAGE.
abstract: Identification of the specific cytogenetic abnormality is one of the critical steps for classification of acute myeloblastic leukemia (AML) which influences the selection of appropriate therapy and provides information about disease prognosis. However at present, the genetic complexity of AML is only partially understood. To obtain a comprehensive, unbiased, quantitative measure, we performed serial analysis of gene expression (SAGE) on CD15(+) myeloid progenitor cells from 22 AML patients who had four of the most common translocations, namely t(8;21), t(15;17), t(9;11), and inv(16). The quantitative data provide clear evidence that the major change in all these translocation-carrying leukemias is a decrease in expression of the majority of transcripts compared with normal CD15(+) cells. From a total of 1,247,535 SAGE tags, we identified 2,604 transcripts whose expression was significantly altered in these leukemias compared with normal myeloid progenitor cells. The gene ontology of the 1,110 transcripts that matched known genes revealed that each translocation had a uniquely altered profile in various functional categories including regulation of transcription, cell cycle, protein synthesis, and apoptosis. Our global analysis of gene expression of common translocations in AML can focus attention on the function of the genes with altered expression for future biological studies as well as highlight genes/pathways for more specifically targeted therapy.
authors.affiliation: Department of Medicine, University of Chicago, IL 60637, USA.
authors.forename: Sanggyu
authors.initials: S
authors.lastname: Lee
authors.forename: Jianjun
authors.initials: J
authors.lastname: Chen
authors.forename: Guolin
authors.initials: G
authors.lastname: Zhou
authors.forename: Run Zhang
authors.initials: RZ
authors.lastname: Shi
authors.forename: Gerard G
authors.initials: GG
authors.lastname: Bouffard
authors.forename: Masha
authors.initials: M
authors.lastname: Kocherginsky
authors.forename: Xijin
authors.initials: X
authors.lastname: Ge
authors.forename: Miao
authors.initials: M
authors.lastname: Sun
authors.forename: Nimanthi
authors.initials: N
authors.lastname: Jayathilaka
authors.forename: Yeong Cheol
authors.initials: YC
authors.lastname: Kim
authors.forename: Neelmini
authors.initials: N
authors.lastname: Emmanuel
authors.forename: Stefan K
authors.initials: SK
authors.lastname: Bohlander
authors.forename: Mark
authors.initials: M
authors.lastname: Minden
authors.forename: Justin
authors.initials: J
authors.lastname: Kline
authors.forename: Ozden
authors.initials: O
authors.lastname: Ozer
authors.forename: Richard A
authors.initials: RA
authors.lastname: Larson
authors.forename: Michelle M
authors.initials: MM
authors.lastname: LeBeau
authors.forename: Eric D
authors.initials: ED
authors.lastname: Green
authors.forename: Jeffery
authors.initials: J
authors.lastname: Trent
authors.forename: Theodore
authors.initials: T
authors.lastname: Karrison
authors.forename: Piu Paul
authors.initials: PP
authors.lastname: Liu
authors.forename: San Ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Janet D
authors.initials: JD
authors.lastname: Rowley
mesh_terms: D017209:Apoptosis; D002454:Cell Differentiation; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D019295:Computational Biology; D018076:DNA, Complementary / Q000378:metabolism; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D016256:Lewis X Antigen / Q000096:biosynthesis; D023461:Myeloid Progenitor Cells / Q000166:cytology; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014178:Translocation, Genetic
chemical_list: D018076:DNA, Complementary; D016256:Lewis X Antigen; D012333:RNA, Messenger; D012313:RNA