Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale.
Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99% for both tests) and specificities (100% for both tests) were confirmed with sera from cattle positive or negative for A. marginale antibodies, respectively, by immunofluorescent antibody test. By the analysis of 583 sera from cattle of three regions of the state of Pernambuco, Brazil, the agreement between both tests was high, with a kappa index of 0.89. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.
Araújo
Flábio R
FR
Embrapa Gado de Corte, Rodovia BR 262, 79002-970 Campo Grande, MS, Brazil. flabio@cnpgc.embrapa.br
Melo
Valeska S P
VS
Ramos
Carlos A N
CA
Madruga
Claudio R
CR
Soares
Cleber O
CO
Kessler
Raul H
RH
Almeida
Nalvo F
NF
Araújo
Graziela S
GS
Alves
Leucio C
LC
Torres Júnior
Roberto A A
RA
Fragoso
Stênio P
SP
Arauco
Paulo R C
PR
Bacanelli
Gisele
G
Oliveira
Maristeli B
MB
Santos
Lenita R
LR
D042322:Anaplasma marginale / Q000276:immunology*; D000712:Anaplasmosis / Q000175:diagnosis*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005455:Fluorescent Antibody Technique; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins; C449089:major surface protein 1a, Anaplasma; C103348:msp2 protein, Anaplasma marginale