Release of mitochondrial DNA fragments from brain mitochondria of irradiated mice.
Recently, we demonstrated that the release of mtDNA fragments from mitochondria occurs as a result of the opening of a non-specific pore in the inner mitochondrial membrane. Here, we show that irradiation of mice stimulates the appearance of mtDNA fragments in the cytosolic fractions of the brain. The fragments of mtDNA were found as early as 1h after irradiation, when no observable alteration of mitochondrial functioning occurred. The involvement of mitochondrial permeability transition in this process is discussed.
Patrushev
Maxim
M
Institute of Theoretical and Experimental Biophysics, Pushchino 142290, Russian Federation.
Kasymov
Vitaliy
V
Patrusheva
Valeria
V
Ushakova
Tatiana
T
Gogvadze
Vladimir
V
Gaziev
Azhub I
AI
D000818:Animals; D001921:Brain / Q000528:radiation effects*; D001923:Brain Chemistry; D003600:Cytosol / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000032:analysis / Q000528:radiation effects*; D005720:Gamma Rays; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000235:genetics / Q000528:radiation effects*
D004272:DNA, Mitochondrial