title: Crystal structure of a Cbf5-Nop10-Gar1 complex and implications in RNA-guided pseudouridylation and dyskeratosis congenita.
abstract: H/ACA RNA-protein complexes, comprised of four proteins and an H/ACA guide RNA, modify ribosomal and small nuclear RNAs. The H/ACA proteins are also essential components of telomerase in mammals. Cbf5 is the H/ACA protein that catalyzes isomerization of uridine to pseudouridine in target RNAs. Mutations in human Cbf5 (dyskerin) lead to dyskeratosis congenita. Here, we describe the 2.1 A crystal structure of a specific complex of three archaeal H/ACA proteins, Cbf5, Nop10, and Gar1. Cbf5 displays structural properties that are unique among known pseudouridine synthases and are consistent with its distinct function in RNA-guided pseudouridylation. We also describe the previously unknown structures of both Nop10 and Gar1 and the structural basis for their essential roles in pseudouridylation. By using information from related structures, we have modeled the entire ribonucleoprotein complex including both guide and substrate RNAs. We have also identified a dyskeratosis congenita mutation cluster site within a modeled dyskerin structure.
authors.affiliation: Department of Chemistry and Biochemistry, Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306, USA.
authors.forename: Rumana
authors.initials: R
authors.lastname: Rashid
authors.forename: Bo
authors.initials: B
authors.lastname: Liang
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Baker
authors.forename: Osama A
authors.initials: OA
authors.lastname: Youssef
authors.forename: Yang
authors.initials: Y
authors.lastname: He
authors.forename: Kathleen
authors.initials: K
authors.lastname: Phipps
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Terns
authors.forename: Michael P
authors.initials: MP
authors.lastname: Terns
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D019871:Dyskeratosis Congenita / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D006836:Hydro-Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D008958:Models, Molecular; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D009154:Mutation; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011560:Pseudouridine / Q000378:metabolism; D020027:Pyrococcus furiosus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020718:Ribonucleoproteins, Small Nucleolar / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D019843:Archaeal Proteins; D018797:Cell Cycle Proteins; C112148:DKC1 protein, human; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D020718:Ribonucleoproteins, Small Nucleolar; D011560:Pseudouridine; D012313:RNA; D006836:Hydro-Lyases; C022479:pseudouridylate synthetase