Suppression of porcine arterivirus replication by baculovirus-delivered shRNA targeting nucleoprotein.
The ability to synthesize shRNAs from DNA templates driven by RNA polymerase III promoters has made it possible to apply virus-derived vectors as delivery vehicles for double-strand RNA-mediated interference. Baculovirus emerges as a promising vector for in vivo gene therapy most recently. To investigate its potential as a delivery vector for anti-virus shRNA targeting arterivirus porcine reproductive and respiratory syndrome virus (PRRSV), we constructed recombinant baculovirus vectors bearing a shRNA-synthesizing cassette driven by U6 promoter with enhanced transduction efficiency by displaying vesicular stomatitis virus glycoprotein on viral envelope. Transduction of Marc145 cells with a recombinant baculovirus delivering egfp gene-specific shRNA dramatically suppressed the expression of EGFP in this cell line; and transduction of Marc145 cells with a baculovirus delivering shRNA specific for the C-terminal nucleoprotein coding region of PRRSV genome resulted in inhibition of viral replication. Our data highlight the recombinant baculovirus as an alternative vehicle for anti-virus shRNA delivery.
Lu
Liqun
L
Animal Health Biotechnology Unit, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore.
Ho
Yuenfern
Y
Kwang
Jimmy
J
D016367:Baculoviridae / Q000235:genetics*; D020868:Gene Silencing; D018390:Gene Targeting / Q000379:methods*; D009698:Nucleoproteins / Q000235:genetics*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000254:growth & development*; D012367:RNA, Viral / Q000235:genetics*; D013489:Suppression, Genetic / Q000235:genetics; D014162:Transfection / Q000379:methods; D014779:Virus Replication / Q000235:genetics*
D009698:Nucleoproteins; D012367:RNA, Viral