Changes of skeletal muscle adiponectin content in diet-induced insulin resistant rats.
The current study examined the relationship between skeletal muscle levels of adiponectin and parameters of insulin sensitivity. A high fat/sucrose diet (HFD) for 20 weeks resulted in significant increases in body weight, serum insulin, triglycerides (TG), and free fatty acids (FFA) (all p < 0.01). Interestingly, this diet leads to a slight increase in serum adiponectin, but significant decreases in gastrocnemius muscle and white adipose adiponectin (all p < 0.05). HFD for 4 weeks also resulted in a significant decrease in muscle adiponectin, which correlated with serum insulin, TG, and FFA (all p < 0.05). Treatment of the 4-week HFD rats with a PPARgamma agonist GI262570 ameliorated the diet-induced hyperinsulinemia and dyslipidemia, and effectively restored muscle adiponectin (all p < 0.05). This study demonstrated that HFD-induced hyperinsulinemia and dyslipidemia appeared without changes in serum adiponectin, but were associated with decreased tissue adiponectin. This provides the first evidence for a connection between tissue adiponectin and diet-induced hyperinsulinemia and dyslipidemia.
Yang
Baichun
B
GlaxoSmithKline, Research Triangle Park, NC 27709, USA. baichum.w.yang@gsk.com
Chen
Lihong
L
Qian
Ying
Y
Triantafillou
James A
JA
McNulty
Judi A
JA
Carrick
Kevin
K
Clifton
Lisa G
LG
Han
Bajin
B
Geske
Robert
R
Strum
Jay
J
Brown
Kathleen K
KK
Stimpson
Stephen A
SA
Pahel
Greg
G
D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D004041:Dietary Fats / Q000378:metabolism*; D019422:Dietary Sucrose / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D010080:Oxazoles / Q000008:administration & dosage; D047495:PPAR gamma / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014443:Tyrosine / Q000008:administration & dosage / Q000031:analogs & derivatives
D052242:Adiponectin; D004041:Dietary Fats; D019422:Dietary Sucrose; D010080:Oxazoles; D047495:PPAR gamma; C422415:farglitazar; D014443:Tyrosine