Serum sphinganine and the sphinganine to sphingosine ratio as a biomarker of dietary fumonisins during chronic exposure in ducks.
Sphinganine concentration (Sa) and sphinganine to sphingosine ratio (Sa/So) are sensitive biomarkers of fumonisin B1 (FB1) exposure in animals and have been proposed to reveal FB1 exposure in humans. They correlate with liver and kidney toxicity and often precede signs of toxicity. However, the use of Sa and Sa/So is confusing during chronic exposure. Indeed, some authors report altered sphingolipids metabolism, whereas others fail to demonstrate significant effect. The aim of this study was to investigate the kinetics of Sa and Sa/So in the serum of ducks over a 77-day exposure to 0, 2, 8, 32 and 128 mg FB1/kg feeds. Serum biochemistry was also investigated to reveal hepatotoxicity. The results obtained indicate that the kinetics of sphingolipids and serum biochemistry are closely linked with the duration of the exposure. After a strong and rapid increase Sa and Sa/So decrease then stabilize. The lowest investigated dose able to determine a detectable effect is 2 mg/kg feeds, the Sa/So ratio being the most sensitive biomarker of FB1 exposure.
Tran
S T
ST
Department of Mycotoxicology, National Veterinary School of Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France.
Tardieu
D
D
Auvergne
A
A
Bailly
J D
JD
Babilé
R
R
Durand
S
S
Benard
G
G
Guerre
P
P
D000818:Animals; D015415:Biomarkers / Q000097:blood; D002274:Carcinogens, Environmental / Q000493:pharmacokinetics* / Q000633:toxicity; D019963:Clinical Chemistry Tests; D004032:Diet; D004305:Dose-Response Relationship, Drug; D004372:Ducks; D037341:Fumonisins / Q000493:pharmacokinetics* / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008111:Liver Function Tests; D009183:Mycotoxins; D009929:Organ Size / Q000187:drug effects; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000097:blood*; D018675:Toxicity Tests / Q000379:methods
D015415:Biomarkers; D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1; D013110:Sphingosine; C005682:safingol