An aqueous polysaccharide extract from the edible mushroom Pleurotus ostreatus induces anti-proliferative and pro-apoptotic effects on HT-29 colon cancer cells.
Anti-proliferative and pro-apoptotic activities of fractions of Pleurotus ostreatus were examined using HT-29 colon cancer cells in vitro. A hot-water-soluble fraction of the mycelium of the liquid cultured mushroom was partially isolated and chemically characterized as a low-molecular-weight alpha-glucan. HT-29 cells were exposed to the different isolates and significant inhibition of proliferation was obtained in a dose-dependent manner. Proliferation inhibition was shown to be the result of apoptotic induction because the pro-apoptotic molecules Bax and cytosolic cytochrome-c were upregulated. Fluorescence-activated cell sorter analyses of polysaccharide-treated HT-29 cells showed a high percentage of Annexin-positive cells. Here, we describe a newly identified low-molecular-weight alpha-glucan with promising anti-tumorigenic properties, and demonstrate its direct effect on colon cancer cell proliferation via induction of programmed cell death.
Lavi
Iris
I
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel.
Friesem
Dana
D
Geresh
Shimona
S
Hadar
Yitzhak
Y
Schwartz
Betty
B
D017304:Annexin A5 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D045304:Cytochromes c / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D005936:Glucans / Q000494:pharmacology*; D019073:HT29 Cells / Q000187:drug effects; D006801:Humans; D008970:Molecular Weight; D010936:Plant Extracts / Q000494:pharmacology*; D020076:Pleurotus / Q000737:chemistry*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D017304:Annexin A5; D000970:Antineoplastic Agents; D005936:Glucans; D010936:Plant Extracts; D051028:bcl-2-Associated X Protein; D045304:Cytochromes c