[Platelet aggregation in modulation of the pathway L-arginine-NO in rats with cerebral ischemia].
Platelet aggregation in the plasma of rats with brain ischemia and modulation of L-arginine-NO pathway were studied in experiments (Born method using analyzer AP 21103AO SOLAR). We used L-arginine and inhibitors of NO-synthase: nonselective inhibitor Nomega-nitro-L-arginine methyl ester, selective inhibitors of neuronal NO-synthase-7-nitro-indazole and selective inhibitor of inducible NO-synthase-S-methylisothiourea. We found that an increase in platelet aggregation in rat plasma observed in both periods is mediated via NO: in an early period of brain ischemia it depends on neuronal NO-synthase and in a late period--on inducible NO-synthase. Moreover, a nonselective inhibitor of all isoforms of NO-synthase (including endothelial NO-synthase) Nomega-nitro-L-arginine methyl ester possesses proaggregant properties. On the other hand, L-arginine in combination with 7-nitro-indazole and S-methylisothiourea induces maximal antiaggregatory effect. These data allows us to suppose that endothelium-derived NO decreases platelet aggregation in brain ischemia-reperfusion in rats.
Maksimovich
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D000818:Animals; D001120:Arginine / Q000378:metabolism* / Q000494:pharmacology; D002545:Brain Ischemia / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007191:Indazoles / Q000494:pharmacology; D007550:Isothiuronium / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*; D051381:Rats
D004791:Enzyme Inhibitors; D007191:Indazoles; D007550:Isothiuronium; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; C027744:S-methylisothiopseudouronium; C080122:7-nitroindazole; D019331:NG-Nitroarginine Methyl Ester