title: A bromoenol lactone suicide substrate inactivates group VIA phospholipase A2 by generating a diffusible bromomethyl keto acid that alkylates cysteine thiols.
abstract: Phospholipases A2 (PLA2) comprise a superfamily of enzymes that hydrolyze phospholipids to a free fatty acid, e.g., arachidonate, and a 2-lysophospholipid. Dissecting their individual functions has relied in large part on pharmacological inhibitors that discriminate among PLA2. Group VIA PLA2 (iPLA2beta) has a GTSTG serine lipase consensus sequence, and studies with a bromoenol lactone (BEL) suicide substrate inhibitor have been taken to suggest that iPLA2beta participates in a wide variety of biological processes. Such conclusions presume inhibitor specificity. Inhibition by BEL requires its hydrolysis by and results in uncharacterized covalent modification(s) of iPLA2beta. We performed mass spectrometric analyses of proteolytic digests of BEL-treated iPLA2beta to identify modifications associated with loss of activity. The GTSTG active site and large flanking regions of sequence are not modified by BEL treatment, but most iPLA2beta Cys residues are alkylated at various BEL concentrations to form a thioether linkage to a BEL keto acid hydrolysis product. Synthetic Cys-containing peptides are alkylated when incubated with iPLA2beta and BEL, which reflects iPLA2beta-catalyzed BEL hydrolysis to a diffusible bromomethyl keto acid product that reacts with distant thiols. The BEL concentration dependence of Cys651 alkylation closely parallels that of loss of iPLA2beta activity. No amino acid residues other than Cys were found to be modified, suggesting that Cys alkylation is the covalent modification of iPLA2beta responsible for loss of activity, and the alkylating species appears to be a diffusible hydrolysis product of BEL rather than a tethered acyl-enzyme intermediate.
authors.affiliation: Medicine Department Mass Spectrometry Facility, Division of Endocrinology, Metabolism, and Lipid Research, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
authors.forename: Haowei
authors.initials: H
authors.lastname: Song
authors.forename: Sasanka
authors.initials: S
authors.lastname: Ramanadham
authors.forename: Shunzhong
authors.initials: S
authors.lastname: Bao
authors.forename: Fong-Fu
authors.initials: FF
authors.lastname: Hsu
authors.forename: John
authors.initials: J
authors.lastname: Turk
mesh_terms: D000595:Amino Acid Sequence; D016367:Baculoviridae / Q000235:genetics; D002478:Cells, Cultured; D003545:Cysteine / Q000737:chemistry*; D004058:Diffusion; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D054501:Group II Phospholipases A2; D006842:Hydrocarbons, Brominated / Q000737:chemistry*; D007651:Keto Acids / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000378:metabolism*; D010741:Phospholipases A / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D054467:Phospholipases A2; D011753:Pyrones / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D006842:Hydrocarbons, Brominated; D007651:Keto Acids; D009281:Naphthalenes; D011753:Pyrones; D011994:Recombinant Proteins; D013438:Sulfhydryl Compounds; C068314:6-(bromomethylene)tetrahydro-3-(1-naphthaleneyl)-2H-pyran-2-one; C005218:methyl bromide; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; D003545:Cysteine