title: Expression of the testis-specific gene, TSGA10, in Iranian patients with acute lymphoblastic leukemia (ALL).
abstract: Testis-specific gene antigen (TSGA10) is expressed in fetus, testis and frequently in human solid cancers and acute leukemias, making it a candidate for immunotherapy and for detection of minimal residual disease (MRD). This gene is considered as a member of cancer-testis (CT) genes. We previously demonstrated TSGA10 expression during spermatogenesis. There is also evidence for potential TSGA10 involvement in cell proliferation. TSGA10 expression has been observed in a wide spectrum of cancers but not in hematopoietic neoplasm. Here we demonstrated expression of TSGA10 by semi-quantitative RT-PCR in 44 (84.6%) out of 52 bone marrow samples and all peripheral blood samples from patients with acute lymphoblastic leukemia (ALL). Twenty-seven (52%) cases had high level of gene expression and 16 (30.7%) cases had a lower expression level of the gene in the patients bone marrow. Presence of TSGA10 expression in ALL may open a window to functional study of mitotic checkpoint proteins in leukemia. RT-PCR of TSGA10 may help in detection of residual clonal cells leading to early diagnosis and better prognostic qualification of the disease.
authors.affiliation: Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam Beigom
authors.initials: MB
authors.lastname: Mobasheri
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Modarressi
authors.forename: Mahdi
authors.initials: M
authors.lastname: Shabani
authors.forename: Hossein
authors.initials: H
authors.lastname: Asgarian
authors.forename: Ramezan-Ali
authors.initials: RA
authors.lastname: Sharifian
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Vossough
authors.forename: Fazel
authors.initials: F
authors.lastname: Shokri
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D003598:Cytoskeletal Proteins; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic / Q000235:genetics*; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000453:epidemiology / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D003598:Cytoskeletal Proteins; D011506:Proteins; C429756:TSGA10 protein, human