title: Modulatory effect of dietary factors on iron-induced in vivo lipid peroxidation in liver and serum of albino rats.
abstract: Effect of a computed diet based on cereals and spices incorporated with either crude palm oil (CPO)/soybean oil (SBO)/cod liver oil (CLO) at 10% level in the diet in modulating iron-induced in vivo lipid peroxidation was carried out during a 12 week study in albino rats. Three groups of rats, each divided into three sets were fed diets based on casein/ragi/jowar incorporated with CPO or SBO or CLO. The casein group of rats did not receive any spice mixture, while the ragi and jowar groups received spice mixture at 2.5% level. Serum lipid analysis showed significant increase in cholesterol, LDL-c + VLDL-c and decrease in HDL-c levels in all the iron treated group of animals. In liver, non-significant increase in total cholesterol triglyceride and decrease in phospholipid levels were noted. Fatty acid profile of liver tissue exhibited low 18:2 levels in various experimental groups due to peroxidation of membrane lipids. Histopathological examination of liver tissue in particular showed mild cytoplasmic vacuolation in control group of rats fed ragi/jowar and moderate vacuolation in all the iron-treated groups. The results demonstrate that different dietary components can beneficially modulate free radical mediated oxidative stress induced by lipid peroxidation.
authors.affiliation: Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore 570 011, India. drr_chandran@yahoo.com
authors.forename: H D
authors.initials: HD
authors.lastname: Ramachandran
authors.forename: R
authors.initials: R
authors.lastname: Kusum
authors.forename: P L
authors.initials: PL
authors.lastname: Raina
mesh_terms: D000818:Animals; D004032:Diet; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage; D007501:Iron / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004042:Dietary Fats, Unsaturated; D008055:Lipids; D007501:Iron