title: A comprehensive description of the transcriptome of the hypothalamoneurohypophyseal system in euhydrated and dehydrated rats.
abstract: The hypothalamoneurohypophyseal system (HNS) consists of the large peptidergic magnocellular neurons of the supraoptic hypo thalamic nucleus (SON) and the paraventricular hypothalamic nucleus (PVN), the axons of which course through the internal zone of the median eminence and terminate at blood capillaries of the posterior lobe of the pituitary gland. The HNS is a specialized brain neurosecretory apparatus responsible for the production of the antidiuretic peptide hormone vasopressin (VP). VP maintains water balance by promoting water conservation at the level of the kidney. Dehydration evokes a massive increase in the regulated release of VP from magnocellular neuron axon terminals in the posterior pituitary, which is accompanied by a plethora of changes in the morphology, electrophysiological properties, and biosynthetic and secretory activity of the HNS. We wish to understand this functional plasticity in terms of the differential expression of genes. We have therefore used microarrays to comprehensively catalog the genes expressed in the PVN, the SON and the neurointermediate lobe of the pituitary gland of control and dehydrated rats. Comparison of these gene lists has enabled us to identify transcripts that are regulated as a consequence of dehydration as well as RNAs that are enriched in the PVN or the SON. We suggest that these differentially expressed genes represent candidate regulators and effectors of HNS activity and remodeling.
authors.affiliation: The Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, England.
authors.forename: Charles
authors.initials: C
authors.lastname: Hindmarch
authors.forename: Song
authors.initials: S
authors.lastname: Yao
authors.forename: Gemma
authors.initials: G
authors.lastname: Beighton
authors.forename: Julian
authors.initials: J
authors.lastname: Paton
authors.forename: David
authors.initials: D
authors.lastname: Murphy
mesh_terms: D000818:Animals; D001369:Axons; D001921:Brain / Q000378:metabolism; D001931:Brain Mapping / Q000379:methods; D003681:Dehydration; D015536:Down-Regulation; D004594:Electrophysiology; D005786:Gene Expression Regulation; D007031:Hypothalamus / Q000378:metabolism / Q000473:pathology*; D007668:Kidney / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010904:Pituitary Gland, Posterior / Q000378:metabolism / Q000473:pathology*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D015854:Up-Regulation; D014667:Vasopressins / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D014867:Water; D014667:Vasopressins; D012313:RNA