Batch introduction techniques.
Mass spectrometry (MS) is widely used as a rapid tool for peptide profiling and protein identification. However, the success of the method is compromised by dirty and contaminated samples. Moreover, analysis from a small sample volume with a relative high concentration is usually required. In this chapter, different microscale sample preparation methods are discussed for off-line, matrix-assisted laser desorption/ionization (MALDI) and nanoelectrospray ionization (nanoESI) MS analysis.
Jiménez
Connie R
CR
Mass Spectrometry Resource, Department of Pharmaceutical Chemistry, University of California, San Francisco, USA.
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods*; D009479:Neuropeptides / Q000737:chemistry; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry; D010902:Pituitary Gland / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000737:chemistry; D051381:Rats; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013997:Time Factors
D009479:Neuropeptides; D010455:Peptides; D011506:Proteins; D012997:Solvents