The effect of DNA priming-protein boosting on enhancing humoral immunity and protecting mice against lethal HSV infections.
Herpes simplex virus produces primary and latent infections with periodic recurrency. The prime-boost immunization strategies were studied using a DNA vaccine carrying the full-length glycoprotein D-1 gene and a baculovirus-derived recombinant glycoprotein D, both expressing herpes simplex virus glycoprotein D-1 protein. Immunization with recombinant DNAs encoding antigenic proteins could induce cellular and humoral responses by providing antigen expression in vivo. Higher immune response, however, occurred when the recombinant proteins followed DNA inoculation. While all groups of the immunized mice and positive control group could resist virus challenge, a higher virus neutralizing antibody level was detected in the animals receiving recombinant protein following DNA vaccination.
Soleimanjahi
Hoorieh
H
Department of Virology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran. soleim_h@modares.ac.ir
Roostaee
Mohammad Hassan
MH
Rasaee
Mohammad Javad
MJ
Mahboudi
Fereidoon
F
Kazemnejad
Anooshirvan
A
Bamdad
Taravat
T
Zandi
Keivan
K
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation / Q000187:drug effects; D019556:COS Cells; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000276:immunology* / Q000494:pharmacology; D005260:Female; D006561:Herpes Simplex / Q000517:prevention & control*; D007117:Immunization, Secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D018139:Simplexvirus / Q000235:genetics / Q000276:immunology*; D019444:Vaccines, DNA / Q000627:therapeutic use*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014765:Viral Vaccines / Q000627:therapeutic use*; D014779:Virus Replication
D000914:Antibodies, Viral; D004279:DNA, Viral; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C039447:glycoprotein D, Human herpesvirus 1