[Establishment of high-throughput drug screening model targeting retinoic acid receptor in cells].
OBJECTIVE
To screen new drug for the treatment of acute promyelocytic leukemia, psoriasis and acne, high-throughput drug screening cell models marked by green fluorescent protein (GFP) have been established.


METHODS
Eight repeats of retinoic acid response element (RARE) were synthesized and cloned into a GFP expression vector. This construct was stably transfected into cells in vitro. Stable and sensitive cell clones with high copy numbers of RARE were selected by retinoic acid (RA) using fluorescence-activated cell sorting (FACS).


RESULTS
A cell line has been chosen to be high-throughput drug screening cell model. This model was shown with low background, high sensitive and good reproducibility, and was convenient and inexpensive.


CONCLUSIONS
This drug screening cell model can be used for retinoic acid receptor target high-throughput drug screening.
Guo
Feng
F
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China.
Liu
Yan-Xin
YX
Zheng
De-Xian
DX
Jiang
Cheng-Yu
CY
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004622:Embryo, Mammalian; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D010957:Plasmids; D018168:Receptors, Retinoic Acid / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D014162:Transfection; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology*
D000970:Antineoplastic Agents; D018168:Receptors, Retinoic Acid; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D014212:Tretinoin