title: Vitamin E inhibits hemolysis induced by hemin as a membrane stabilizer.
abstract: Hemin is a potential cytolytic agent. To test the effect of vitamin E on hemin-mediated permeability in cell membranes, sheep erythrocytes were chosen as an appropriate model to study hemolysis induced by hemin. Hemin-induced hemolysis but did not elicit lipid peroxidation in sheep erythrocytes. Vitamin E was effective in inhibiting hemin-mediated hemolysis. Both chromanol ring and the isoprenoid side chain of tocopherols were essential for inhibition of hemin-induced hemolysis. There was a strong correlation between the inhibitory effects of tocopherols on hemin-induced erythrocyte hemolysis and their effects on fluorescence anisotropy of cell membranes. Our results suggested that, in contrast to its antioxidant activity, vitamin E inhibits hemolysis induced by hemin as a membrane stabilizing agent.
authors.affiliation: School of Life Sciences, Yunnan University, Kunming, Yunnan 650091, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Tinghua
authors.initials: T
authors.lastname: Wang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Lai
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Chenggang
authors.initials: C
authors.lastname: Zou
mesh_terms: D000818:Animals; D016880:Anisotropy; D000975:Antioxidants / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D004912:Erythrocytes / Q000187:drug effects* / Q000473:pathology; D005454:Fluorescence Polarization; D006427:Hemin / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D008560:Membrane Fluidity / Q000187:drug effects; D012756:Sheep; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D006427:Hemin