title: The determination of nicotinic acid in plasma by mixed-mode liquid chromatography-tandem mass spectrometry following ion exchange solid phase extraction.
abstract: An assay for nicotinic acid in plasma samples has been developed using ion exchange solid phase extraction in 96-well format followed by mixed-mode ion exchange/reversed-phase liquid chromatography with positive ion tandem mass spectrometry detection. The assay avoids the need for time-consuming derivatisation procedures or involatile ion-pair chromatography reagents. The assay is linear over the wide range 0.05-20 microg/mL, based on a 100 microL sample (correlation coefficient>0.99). The assay is accurate and precise (bias and coefficient of variation<18%) over this calibration range.
authors.affiliation: Drug Metabolism and Pharmacokinetics, Cardiovascular and Urogenital Centre of Excellence in Drug Discovery, GlaxoSmithKline R&D, Gunnels Wood Road, Stevenage, Herts, UK. dave.n.mallett@gsk.com
authors.forename: D N
authors.initials: DN
authors.lastname: Mallett
authors.forename: S
authors.initials: S
authors.lastname: Dayal
authors.forename: G J
authors.initials: GJ
authors.lastname: Dear
authors.forename: A J
authors.initials: AJ
authors.lastname: Pateman
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods; D013058:Mass Spectrometry; D009525:Niacin / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results
chemical_list: D009525:Niacin