Occurrence of co-infection by Leishmania (Leishmania) chagasi and Trypanosoma (Trypanozoon) evansi in a dog in the state of Mato Grosso do Sul, Brazil.
A natural case of co-infection by Leishmania and Trypanosoma is reported in a dog (Canis familiaris) in south- western state of Mato Grosso do Sul, Brazil. Both amastigote and trypomastigote forms were observed after Giemsa staining of cytological preparations of the dog's bone marrow aspirate. No parasite was detected using medium culture inoculation of the sample. DNA obtained from the bone marrow aspirate sample and from the blood buffy coat was submitted to polymerase chain reaction (PCR) with a set of rDNA-based primers S4/S12. The nucleotide sequence of the PCR product was identical to that of Trypanosoma (Trypanozoon) evansi. The S4/S12 PCR was then used as template in a nested-PCR using a specific Leishmania set S17/S18 as primers, to explain the amastigote forms. The nucleotide sequence of the new PCR product was identical to that of Leishmania (Leishmania) chagasi. This case, as far as we know, is the first report of a dog co-infected with these parasites, suggesting that besides L. (L.) chagasi, the natural transmission of T. (T.) evansi occurs in the area under study.
Savani
Elisa San Martin Mouriz
ES
Laboratório de Zoonoses e Doenças Transmitidas por Vetores da Vigilância em Saúde, Prefeitura do Município de São Paulo, 02031-020 São Paulo, SP, Brazil. elisasanmar@yahoo.com.br
Nunes
Vania Lúcia Brandão
VL
Galati
Eunice Aparecida Bianchi
EA
Castilho
Tiago Moreno
TM
Araujo
Fernando Shiroma de
FS
Ilha
Iêda Maria Novaes
IM
Camargo
Maria Cecília Gibrail de Oliveira
MC
D'Auria
Sandra Regina Nicoletti
SR
Floeter-Winter
Lucile Maria
LM
D000818:Animals; D001938:Brazil; D016054:DNA, Protozoan / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D018314:Leishmania infantum / Q000235:genetics / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D014345:Trypanosoma / Q000235:genetics / Q000276:immunology; D014352:Trypanosomiasis / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*
D016054:DNA, Protozoan; D004275:DNA, Ribosomal