Temperature increase results in oxidative stress in goldfish tissues. 2. Antioxidant and associated enzymes.
Activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GR), and glucose-6-phophate dehydrogenase (G6PDH) were measured in four tissues of goldfish, Carassius auratus L., over 1-12 h of high temperature (35 degrees C) exposure followed by 4 or 24 h of lower temperature (21 degrees C) recovery. SOD activity was strongly affected by heat shock, increasing 4-fold in brain, liver, and kidney, but was mainly reversed at recovery. In some tissues, activities of SOD, catalase, GPx, and G6PDH decreased significantly after 1 h heat shock exposure suggesting that thermal inactivation possibly occurred, but were renewed at further exposure. In many cases, 4 h of return to the initial temperature decreased enzyme activities. High correlation coefficients between SOD activities and levels of lipid peroxidation products suggest that these products might be involved in up-regulation of antioxidant defense. Several enzymes (SOD, GST, GR) responded to stress in coordinated manner.
Lushchak
Volodymyr I
VI
Department of Biochemistry, Precarpathian National University named after Vassyl Stefanyk, 57 Shevchenko Str., 76025, Ivano-Frankivsk, Ukraine. lushchak@pu.if.ua
Bagnyukova
Tetyana V
TV
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002374:Catalase / Q000032:analysis; D004798:Enzymes / Q000032:analysis*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D005979:Glutathione Peroxidase / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis; D005982:Glutathione Transferase / Q000032:analysis; D006054:Goldfish / Q000378:metabolism*; D018869:Heat-Shock Response; D006358:Hot Temperature; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000032:analysis
D000975:Antioxidants; D004798:Enzymes; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase