[Study on identification, colonization and reorganization of rice endophytic bacteria].
Endophytic SR-15, SR-25 and SL-37 strains screened from rice hybrid D you 527 in Sichuan were analyzed. Through penetration and microscopic test, the strains were found be mainly located in the cell gap, vacuole and cytoplasm. PUC18 transferring and ERIC-PCR showed that the recombination strain SR-15 could grow in the plant stably. The strain was identified as Bacillus halmapalus based on its chemical components of cell wall, physiological and biochemical characters. It was also shown that the strain was not injurious to rice plant, instead, it promoted rice plant growth by penetration. The Cry1Ac gene was transferred into the stain and verified by Southern analysis. Cry1Ac-transferred SR-15 was toxic to the Chilo suppressalis, brought about death ratio as high as 84.2%.
Zheng
Ai Ping
AP
Sichuan Provincial Center For Agri-Biotech Research.
Sun
Hui Qing
HQ
Li
Ping
P
Tan
Fu Rong
FR
Zheng
Xiu Li
XL
Li
Zhuang
Z
D001407:Bacillus / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure; D000083722:Bacillus thuringiensis Toxins; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics; D015139:Blotting, Southern; D003593:Cytoplasm / Q000382:microbiology / Q000648:ultrastructure; D004731:Endotoxins / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D012275:Oryza / Q000382:microbiology* / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D014617:Vacuoles / Q000382:microbiology / Q000648:ultrastructure
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis