title: The cytoplasmic domain of the F protein of Human respiratory syncytial virus is not required for cell fusion.
abstract: The cytoplasmic domains of the fusion proteins encoded by several viruses play a role in cell fusion and contain sites for palmitoylation associated with viral protein trafficking and virus assembly. The fusion (F) protein of Human respiratory syncytial virus (HRSV) has a predicted cytoplasmic domain of 26 residues containing a single palmitoylated cysteine residue that is conserved in bovine RSV F protein, but not in the F proteins of other pneumoviruses such as pneumonia virus of mice, human metapneumovirus and avian pneumovirus. The cytoplasmic domains in other paramyxovirus fusion proteins such as Newcastle disease virus F protein play a role in fusion. In this study, it was shown that deletion of the entire cytoplasmic domain or mutation of the single cysteine residue (C550S) of the HRSV F protein had no effect on protein processing, cell-surface expression or fusion.
authors.affiliation: Department of Infectious Diseases Research, Centocor Inc., 145 King of Prussia Road, Radnor, PA 19087, USA.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Branigan
authors.affiliation: Department of Infectious Diseases Research, Centocor Inc., 145 King of Prussia Road, Radnor, PA 19087, USA.
authors.forename: Nicole D
authors.initials: ND
authors.lastname: Day
authors.affiliation: Department of Infectious Diseases Research, Centocor Inc., 145 King of Prussia Road, Radnor, PA 19087, USA.
authors.forename: Changbao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Infectious Diseases Research, Centocor Inc., 145 King of Prussia Road, Radnor, PA 19087, USA.
authors.forename: Lester L
authors.initials: LL
authors.lastname: Gutshall
authors.affiliation: Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain.
authors.forename: José A
authors.initials: JA
authors.lastname: Melero
authors.affiliation: Department of Infectious Diseases Research, Centocor Inc., 145 King of Prussia Road, Radnor, PA 19087, USA.
authors.forename: Robert T
authors.initials: RT
authors.lastname: Sarisky
authors.affiliation: Department of Infectious Diseases Research, Centocor Inc., 145 King of Prussia Road, Radnor, PA 19087, USA.
authors.forename: Alfred M Del
authors.initials: AMD
authors.lastname: Vecchio
mesh_terms: D002459:Cell Fusion; D002460:Cell Line; D006801:Humans; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D014760:Viral Fusion Proteins