Interfacing myoglobin to graphite electrode with an electrodeposited nanoporous ZnO film.
A biocompatible, nanoporous ZnO film was prepared on graphite electrode by the simple electrodeposition method. Based on the film's strong adsorption ability and friendly microenvironment, it can be used as a good matrix to immobilize myoglobin (Mb) through simple adsorption. Moreover, the entrapped Mb realized direct electron transfer with the electrode and displayed an elegant catalytic activity toward the reduction of hydrogen peroxide, nitrite, and trichloroacetic acid, by which the mediator-free biosensors could be fabricated. Atomic force microscopy, UV-Vis spectra, electrochemical impedance spectroscopy, and cyclic voltammetry, etc. were used to characterize the nanoporous ZnO film and Mb-modified ZnO film. Compared to other methods, electrodeposition of porous material supplied a more simple and convenient approach to prepare biocompatible materials for biosensors.
Zhao
Ge
G
The Key Lab of Analytical Chemistry for Life Science (Education Ministry of China), Department of Chemistry, Nanjing University, People's Republic of China.
Xu
Jing-Juan
JJ
Chen
Hong-Yuan
HY
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D004595:Electroplating / Q000379:methods*; D006108:Graphite; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D009211:Myoglobin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D015034:Zinc Oxide / Q000737:chemistry*
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D009211:Myoglobin; D006108:Graphite; D015034:Zinc Oxide