Rapid determination of valsartan in human plasma by protein precipitation and high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method for the determination of valsartan in human plasma is reported. The assay is based on protein precipitation with methanol and reversed-phase chromatography with fluorimetric detection. The preparation of a batch of 24 samples takes 20 min. The liquid chromatography was performed on an octadecylsilica column (50 mm x 4 mm, 5 microm particles), the mobile phase consisted of acetonitrile -15 mM dihydrogenpotassium phosphate, pH 2.0 (45:55, v/v). The run time was 2.8 min. The fluorimetric detector was operated at 234/374 nm (excitation/emission wavelength). The limit of quantitation was 98 ng/ml using 0.2 ml of plasma. Within-day and between-day precision expressed by relative standard deviation was less than 5% and inaccuracy did not exceed 8%. The assay was applied to the analysis of samples from a pharmacokinetic study.
Macek
J
J
Pharmakl s.r.o., Seydlerova 2451, CZ-15800 Prague 13, Czech Republic. macek@pharmkl.cz
Klíma
J
J
Ptácek
P
P
D057911:Angiotensin Receptor Antagonists; D001798:Blood Proteins / Q000302:isolation & purification*; D002138:Calibration; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013777:Tetrazoles / Q000097:blood* / Q000493:pharmacokinetics; D014633:Valine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D000068756:Valsartan
D057911:Angiotensin Receptor Antagonists; D001798:Blood Proteins; D013777:Tetrazoles; D000068756:Valsartan; D014633:Valine