[Study on differential proteomic expression in human liver cells stimulated by trichloroethylene with proteomics].
OBJECTIVE
To explore the differential proteomic expression in human liver cells L-02 induced by different dosages of trichloroethylene (TCE).


METHODS
Human liver cells L-02 were treated with different concentrations of TCE and the solvent control (dimethylsulfoxide). The total cellular proteins were separated using 2DE and visualized with silver staining after TCE treatment. The images were analyzed with Image Master 2D Platinum 5.0 analysis software. The differentially expressed protein spots were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-TOF-MS).


RESULTS
Fifteen protein spots with significant difference were found, and went upward or downward or disappeared after the stimulation of TCE with different dosages, which indicated that TCE induced the change of the proteomic expression in the liver cells. The mass spectrum identification and the IPI human database retrieval were used for identifying 9 proteins related to the L-02 Liver cells induced by TCE.


CONCLUSIONS
The result provides an insight to TCE-related molecular mechanism and which might be useful for further study of the TCE-associated proteins and molecular markers.
Liu
Jian-jun
JJ
Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
Huang
Hai-yan
HY
Zhuang
Zhi-xiong
ZX
Li
Xi-yi
XY
Yuan
Jian-hui
JH
Yang
Fan
F
Wei
Qin-zhi
QZ
D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014241:Trichloroethylene / Q000633:toxicity*
D014241:Trichloroethylene