Challenges facing the biologist doing chemical genetics.
The goal of high-throughput screening (HTS) from the perspective of the biologist is to identify a highly specific small molecule that can be used to inhibit a protein in its normal biological context. Although several useful small molecules have been identified with HTS, there are many challenges to be considered when contemplating a screen, especially by those unfamiliar with chemical biology.
Soderholm
Jon
J
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA. jfs@berkeley.edu
Uehara-Bingen
Mike
M
Weis
Karsten
K
Heald
Rebecca
R
D001681:Biological Assay; D055598:Chemical Phenomena; D002621:Chemistry; D019295:Computational Biology; D015195:Drug Design; D004353:Drug Evaluation, Preclinical; D008967:Molecular Biology; D012984:Software