Identification of a polymeric Ig receptor binding phage-displayed peptide that exploits epithelial transcytosis without dimeric IgA competition.
The polymeric Ig receptor (pIgR), also called membrane secretory component (SC), mediates epithelial transcytosis of polymeric immunoglobulins (pIgs). J Chain-containing polymeric IgA (pIgA) and pentameric IgM bind pIgR at the basolateral epithelial surface. After transcytosis, the extracellular portion of the pIgR is cleaved at the apical side, either complexed with pIgs as bound SC or unoccupied as free SC. This transport pathway may be exploited to target bioactive molecules to the mucosal surface. To identify small peptide motifs with specific affinity to human pIgR, we used purified free SC and selection from randomized, cysteine-flanked 6- and 9-mer phage-display libraries. One of the selected phages, called C9A, displaying the peptide CVVWMGFQQVC, showed binding both to human free SC and SC complexed with pIgs. However, the pneumococcal surface protein SpsA (Streptococcus pneumoniae secretory IgA-binding protein), which binds human SC at a site distinct from the pIg binding site, competed with the C9A phage for binding to SC. The C9A phage showed greatly increased transport through polarized Madin-Darby canine kidney cells transfected with human pIgR. This transport was not affected by pIgA nor did it inhibit pIgR-mediated pIgA transcytosis. A free peptide of identical amino acid sequence as that displayed by the C9A phage inhibited phage interaction with SC. This implied that the C9A peptide sequence may be exploited for pIgR-mediated epithelial transport without interfering with secretory immunity.
Braathen
Ranveig
R
Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, University of Oslo, Rikshospitalet University Hospital, N-0027 Oslo, Norway. ranveig.braathen@medisin.uio.no
Sandvik
Anders
A
Berntzen
Gøril
G
Hammerschmidt
Sven
S
Fleckenstein
Burkhard
B
Sandlie
Inger
I
Brandtzaeg
Per
P
Johansen
Finn-Eirik
FE
Lauvrak
Vigdis
V
D000818:Animals; D001435:Bacteriophages / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D003545:Cysteine / Q000737:chemistry; D019281:Dimerization; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D007070:Immunoglobulin A / Q000737:chemistry*; D051379:Mice; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D019056:Receptors, Polymeric Immunoglobulin / Q000737:chemistry*; D013296:Streptococcus pneumoniae / Q000378:metabolism; D014162:Transfection
D007070:Immunoglobulin A; D019151:Peptide Library; D010455:Peptides; D019056:Receptors, Polymeric Immunoglobulin; D003545:Cysteine