Collection of hyperconcentrated platelets with Trima Accel.
OBJECTIVE
Lowering the plasma content in single-donor platelet (PLT) concentrates well below 30% implies the need to collect platelets at very high concentrations. Trima Accel (TA) is validated for collection below 4000 x 10(3) PLTs/microl. We evaluated its performance at 5000 x 10(3) PLTs/microl.


METHODS
Twenty blood donors underwent apheresis with TA twice collecting either a hyperconcentrated or a standard single-donor platelet concentrate with a target platelet concentration of 5000 or 1200 x 10(3) PLTs/microl, respectively. We analysed the collection efficiency, the collection rate and the quality of the collected by-plasma.


RESULTS
We collected 20 hyperconcentrated and 20 standard units containing 2.56 +/- 0.5 and 3.39 +/- 0.4 x 10(11) PLTs at a concentration of 4518 +/- 978 and 1374 +/- 166 x 10(3) PLTs/microl in 45 +/- 8 and 39 +/- 6 min resulting in a collection efficiency of 47.5 +/- 10.0 and 70.7 +/- 7.9% and a collection rate of 5.9 +/- 1.4 and 8.8 +/- 1.5 x 10(9) PLTs/min, respectively (all results expressed as mean +/- standard deviation). The collected by-plasma showed a very high grade of cell purity and a satisfactory recovery of the clotting factors.


CONCLUSIONS
Although TA is a suitable device for PLT collection at very high concentrations, improvements are desirable to further increase the productivity above its currently validated upper collect concentration limit.
Ringwald
J
J
Department of Transfusion Medicine and Haemostaseology, University Hospital of Erlangen, Erlangen, Germany. juergen.ringwald@trans.imed.uni-erlangen.de
Duerler
T
T
Frankow
O
O
Zimmermann
R
R
Zingsem
J
J
Strasser
E
E
Antoon
M
M
Eckstein
R
R
D006801:Humans; D010976:Platelet Count; D010983:Plateletpheresis / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D012984:Software