The efficiency of interference of Potato virus X infection depends on the target gene.
RNA silencing is a natural defense response against viral infection. This phenomenon has been used to interfere with viral infections by exploiting fragments of viral genomes as sources of RNA silencing. Agrobacterium-mediated transient expression of a hairpin RNA derived from the TGBp1 gene of Potato virus X (PVX) induced RNA silencing of the TGBp1 gene and resulted in interference of PVX infection. The interference was induced in the infiltrated leaves but not in the upper non-infiltrated leaves. Transient expression of a CP hairpin RNA also induced interference of PVX. The TGBp1 hairpin RNA showed more efficient interference of PVX infection than the CP hairpin RNA.
Takahashi
Shuichiro
S
Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
Komatsu
Ken
K
Kagiwada
Satoshi
S
Ozeki
Johji
J
Mori
Takuma
T
Hirata
Hisae
H
Yamaji
Yasuyuki
Y
Ugaki
Masashi
M
Namba
Shigetou
S
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D017863:Potexvirus / Q000235:genetics / Q000254:growth & development*; D034622:RNA Interference; D012367:RNA, Viral / Q000096:biosynthesis; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics*
D012367:RNA, Viral; D014764:Viral Proteins