[Mast cells as a source of nitric oxide in the human placenta--morphometric analysis in preeclampsia complicated pregnancy].
The aim of the study was to test hypothesis, that in placenta mast cells are significant source of iNOS.


METHODS
Placentas were collected after term delivery from healthy (control, n=13) and preeclamptic (n=11) women. Mast cells and iNOS expression were detected in obtained samples with monoclonal anti-iNOS and anti-tryptase antibodies. Next, morphometric analysis were performed. There were no significant difference between iNOS expression and number of iNOS-positive cells in normal and preeclamptic placentas. There was a significant increase in mast cells number in preeclamptic placentas (8.32 +/- 1.3 SD vs 5.14 +/- 1.2 SD in control) and decrease in percentage of iNOS positive cells among them (68.1% vs 23.6%). We conclude, that in uncomplicated pregnancies, mast cells are the main source of iNOS in placenta while in preeclampsia they loss their potential to synthetase iNOS.
Szewczyk
Grzegorz
G
Katedra i Zakład Patologii Ogólnej i Doświadczalnej AM w Warszawie.
Szukiewicz
Dariusz
D
Smiertka
Wacław
W
Szewczyk
Agnieszka
A
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D008407:Mast Cells / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D010920:Placenta / Q000737:chemistry* / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy
D052248:Nitric Oxide Synthase Type I