title: Inositol triphosphate and ryanodine receptors in the control of the cholinosensitivity of common snail neurons by the Na,K pump during habituation.
abstract: The effects of the Na,K pump inhibitor ouabain on habituation of the common snail to tactile stimulation were identical to the ouabain-induced modification of the decrease in the cholinosensitivity of defensive behavior command neurons in the common snail in a cellular model of habituation. Studies addressed the effects of intracellularly delivered ligands of two types of Ca2+ depot receptors--inositol triphosphate (IP3) receptors and ryanodine receptors--on the action of ouabain in the cellular analog of habituation. The IP3 receptor antagonist heparin (0.1 mM), the IP3 receptor agonist inositol triphosphate (0.1 mM), and the ryanodine-dependent Ca2+ mobilization inhibitor dantrolene (0.1 mM) prevented ouabain from modifying the depression of the evoked acetylcholine current. The ryanodine agonist/antagonist ryanodine was used at two concentrations (0.1 and 1 mM) and neither had any effect on the action of ouabain. It is concluded that Ca2+ mobilized from intracellular Ca2+ depots via IP3 receptors is involved in the neuronal mechanism of regulation of the habitation of the common snail to tactile stimulation by the Na,K pump.
authors.affiliation: Department of Higher Nervous Activity, M. V. Lomonosov Moscow State University.
authors.forename: V L
authors.initials: VL
authors.lastname: Nistratova
authors.forename: A S
authors.initials: AS
authors.lastname: Pivovarov
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D001522:Behavior, Animal; D015220:Calcium Channels / Q000378:metabolism*; D003620:Dantrolene / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004924:Escape Reaction / Q000187:drug effects / Q000502:physiology; D006185:Habituation, Psychophysiologic / Q000502:physiology*; D006493:Heparin / Q000494:pharmacology; D053496:Inositol 1,4,5-Trisphosphate Receptors; D000072181:Inositol Polyphosphate 5-Phosphatases; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D009475:Neurons, Afferent / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D010042:Ouabain / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000494:pharmacology; D010812:Physical Stimulation / Q000379:methods; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D012433:Ryanodine / Q000494:pharmacology; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*; D012908:Snails; D000254:Sodium-Potassium-Exchanging ATPase / Q000502:physiology*
chemical_list: D015220:Calcium Channels; D004791:Enzyme Inhibitors; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D019837:Ryanodine Receptor Calcium Release Channel; D012433:Ryanodine; D010042:Ouabain; D006493:Heparin; D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases; D000254:Sodium-Potassium-Exchanging ATPase; D003620:Dantrolene; D000109:Acetylcholine