Influence of aconitine-containing herbal extract BC1 on proliferative and electrokinetic characteristics of endothelial cells.
OBJECTIVE
To evaluate the influence of new antitumor preparation BC1 produced on the base of Aconitum species on viability and electrokinetic properties of endothelial cells for estimation of mechanisms of its antitumor and antimetastatic activity.


METHODS
Cytotoxic/cytostatic action of BC1 toward murine aorta endothelial cells (MAEC) and tumor LLC/R9 cells was studied using MTT-test. Apoptotic rate of MAEC was performed by flow cytometry. Electrokinetic properties of MAEC were determined by linear rate of their migration in electric field with the voltage of 20 V/cm.


RESULTS
After 24 h of incubation with BC1, IC50 for actively proliferating was 0.95 -/+ 0.06 microg/ml and was 9-fold and 14-fold lower (p < 0.05) than that index for confluent endotheliocytes and LLC/R9 cells respectively. The ability of BC1 to alter electrokinetic characteristics of MAEC and to induce apoptosis has been demonstrated. At the concentration of IC50/10, 1 caused 2-fold decrease of zeta-potential and surface density of charge of compared to the control (p < 0.05) whilst at the concentration of IC50/20 - inversion of surface charge of the majority (80%) of the cells in association with BC1-induced apoptosis.


CONCLUSIONS
High sensitivity of actively proliferating MAEC to the action of BC1 was revealed as well as the ability of that preparation to cause apoptosis and inversion of surface charge of endothelial cells.
Garmanchouk
L V
LV
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine.
Pyaskovskaya
O N
ON
Yanish
Yu V
YV
Shlyakhovenko
V A
VA
Dasyukevich
O I
OI
Solyanik
G I
GI
D000157:Aconitine / Q000494:pharmacology*; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D042783:Endothelial Cells / Q000187:drug effects*; D051379:Mice
D000276:Adjuvants, Immunologic; D000972:Antineoplastic Agents, Phytogenic; D000157:Aconitine