Ginkgo biloba extract protects against ionizing radiation-induced oxidative organ damage in rats.
The present study was designed to determine the possible protective effects of Ginkgo biloba extract (EGb) against oxidative organ damage induced by irradiation (IR). Sprague-Dawley rats were exposed to whole-body IR (800 cGy) after a 15-day pretreatment with either saline or EGb (50 mg/kg/day), intraperitoneally, and treatments were repeated immediately after the IR. Then the rats were decapitated at either 6 h or 72 h after IR, where EGb or saline injections were repeated once daily. Lung, liver, kidney and ileum samples were obtained for the determination of malondialdehyde, glutathione levels, myeloperoxidase activity and collagen contents, while oxidant-induced DNA fragmentation was evaluated in the ileal tissues. All tissues were also examined microscopically and assayed for the production of reactive oxidants using chemiluminescence (CL). Lactate dehydrogenase (LDH)-an indicator of tissue damage and TNF-alpha were assayed in serum samples. In the saline-treated irradiation groups, glutathione levels were decreased significantly, while the malondialdehyde levels, myeloperoxidase activity and collagen content were increased in the tissues (p < 0.01-0.001), which were in parallel with the increases in luminol and lucigenin CL values. In the EGb treated-IR groups, all of these oxidant responses were prevented significantly (p < 0.05-0.01). LDH and TNF-alpha levels, which were increased significantly (p < 0.01-0.001) following IR, were decreased (p < 0.05-0.001) with EGb treatment. In conclusion, the present data demonstrate that EGb, through its free radical scavenging and antioxidant properties, attenuates irradiation-induced oxidative organ injury, suggesting that EGb may have a potential benefit in enhancing the success of radiotherapy.
Sener
Göksel
G
Marmara University, School of Pharmacy, Department of Pharmacology, Tibbiye Cad. 34668 Istanbul, Turkey. gsener@marmara.edu.tr
Kabasakal
Levent
L
Atasoy
Beste Melek
BM
Erzik
Can
C
Velioğlu-Oğünç
Ayliz
A
Cetinel
Sule
S
Gedik
Nursal
N
Yeğen
Berrak C
BC
D000818:Animals; D017209:Apoptosis; D003094:Collagen / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020441:Ginkgo biloba; D005978:Glutathione / Q000378:metabolism; D007082:Ileum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014916:Whole-Body Irradiation
D016166:Free Radical Scavengers; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D003094:Collagen; D007770:L-Lactate Dehydrogenase; D005978:Glutathione