Systemic RNAi of the cockroach vitellogenin receptor results in a phenotype similar to that of the Drosophila yolkless mutant.
During vitellogenesis, one of the most tightly regulated processes in oviparous reproduction, vitellogenins are incorporated into the oocyte through vitellogenin receptor (VgR)-mediated endocytosis. In this paper, we report the cloning of the VgR cDNA from Blattella germanica, as well as the first functional analysis of VgR following an RNA interference (RNAi) approach. We characterized the VgR, VgR mRNA and protein expression patterns in pre-adult and adult stages of this cockroach, as well as VgR immunolocalization in ovarioles, belonging to the panoistic type. We then specifically disrupted VgR gene function using RNAi techniques. Knockdown of VgR expression led to a phenotype characterized by low yolk content in the ovary and high vitellogenin concentration in the haemolymph. This phenotype is equivalent to that of the yolkless mutant of Drosophila melanogaster, which have the yl (VgR) gene disrupted. The results additionally open the perspective that development genes can be functionally analyzed via systemic RNAi in this basal species.
Ciudad
Laura
L
Department of Physiology and Molecular Biodiversity. Institute of Molecular Biology of Barcelona, Barcelona, Spain.
Piulachs
Maria-Dolors
MD
Bellés
Xavier
X
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D003058:Cockroaches / Q000235:genetics*; D017931:DNA Primers; D004331:Drosophila melanogaster / Q000235:genetics*; D004527:Egg Proteins / Q000235:genetics*; D005260:Female; D020868:Gene Silencing; D008297:Male; D009154:Mutation; D010802:Phylogeny; D034622:RNA Interference; D011956:Receptors, Cell Surface / Q000235:genetics*
D017931:DNA Primers; D004527:Egg Proteins; D011956:Receptors, Cell Surface; C030474:vitellogenin receptor