[Molecular biological survey of tick-born arboviruses in southern part of Xinjiang].
BACKGROUND
To disclose the species and distribution of tick-borne arboviruses in the southern part of Xinjiang.


METHODS
Totally 5045 ticks were collected from 36 collecting sites of 23 places in the southern Xinjiang, which were made into cDNA pools with pd(N)6 primer through RT-PCR method. Then PCR was used to detect viral nucleotide sequence from cDNA.


RESULTS
All 34 cDNAs showed negative to flavivirus and California serogroup virus primers; but nairovirus and primers derived from Xinjiang hemorrhagic fever virus had amplified and yielded some obvious bands corresponding to the nucleotide sequences of Xinjiang hemorrhagic fever virus. A phylogenetic analysis was done to the obtained partial sequences of L and S segments.


CONCLUSIONS
Nucleotide sequences of Neither flaviviruses nor California serogroup viruses were detected from the samples. However partial L segment sequence was first reported in China. Phylogenetic analysis of partial L and S segments disclosed the molecular characteristic of Xinjiang hemorrhagic fever virus.
Lv
Xin-jun
XJ
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Tang
Qing
Q
Feng
Yu-ming
YM
Zhi
Qi
Q
Wang
Cheng
C
Xu
Li-hong
LH
Li
Hao
H
Fu
Shi-hong
SH
Wang
Huan-yu
HY
Jin
Xin
X
Liang
Guo-dong
GD
D000818:Animals; D001103:Arboviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017282:Tick-Borne Diseases / Q000821:virology; D013987:Ticks / Q000821:virology*