Oral vaccination against Helicobacter pylori infection is not effective in mice with Fas ligand deficiency.
The aim of this study was to delineate the role of the Fas pathway in vaccination against Helicobacter pylori. C57BL/6 and Fas ligand-deficient (gld) mice were divided into 3 groups: control, H. pylori infected, and orally vaccinated (H. pylori whole cell sonicate and cholera toxin adjuvant). Oral vaccination prevented H. pylori colonization in 78% of C57BL/6 mice compared to only 18% of gld mice. Vaccination did not alter the degree of apoptosis in either strain of mice. Vaccination led to significant increase in interleukin (IL)-5 and IL-10 in C57BL/6 but not gld mice. H. pylori infection increased interferon (IFN)-gamma levels in C57BL/6 but not in gld mice while vaccination had no effect on IFN-gamma levels in either strain. Oral vaccination is not effective in Fas ligand-deficient mice likely owing to lack of effective cytokine responses. This indicates that the Fas pathway plays a critical role in promoting an appropriate effector response following H. pylori vaccination.
Avitzur
Yaron
Y
Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.
Galindo-Mata
Esther
E
Jones
Nicola L
NL
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology; D001428:Bacterial Vaccines / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D053222:Fas Ligand Protein; D016481:Helicobacter Infections / Q000276:immunology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000276:immunology*; D007378:Interleukins / Q000032:analysis / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000172:deficiency* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D012016:Reference Values; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D017211:Treatment Failure; D048069:Tumor Necrosis Factors / Q000172:deficiency* / Q000276:immunology; D014611:Vaccination / Q000379:methods
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D007378:Interleukins; D008562:Membrane Glycoproteins; D048069:Tumor Necrosis Factors