Methylobacterium extorquens AM1 produces a novel type of acyl-homoserine lactone with a double unsaturated side chain under methylotrophic growth conditions.
Acyl-homoserine lactones (acyl-HSLs) have emerged as important regulatory molecules for many gram-negative bacteria. We have found that Methylobacterium extorquens AM1, a member of the pink-pigmented facultative methylotrophs commonly present on plant surfaces, produces several acyl-HSLs depending upon the carbon source. A novel HSL was discovered with a double unsaturated carbon chain (N-(tetradecenoyl)) (C14:2) and characterized by MS and proton NMR. This long-chain acyl-HSL is synthesized by MlaI that also directs synthesis of C14:1-HSL. The Alphaproteobacterium also produces N-hexanoyl-HSL (C6-HSL) and N-octanoyl-HSL (C8-HSL) via MsaI.
Nieto Penalver
Carlos G
CG
Laboratoire des Interaction Plantes Micro-organismes, INRA/CNRS, BP52627, 31326 Castanet-Tolosan, France.
Morin
Danièle
D
Cantet
Franck
F
Saurel
Olivier
O
Milon
Alain
A
Vorholt
Julia A
JA
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D000432:Methanol / Q000378:metabolism; D020601:Methylobacterium extorquens / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D019802:Succinic Acid / Q000378:metabolism
C088386:homoserine lactone; D002244:Carbon; D019802:Succinic Acid; D015107:4-Butyrolactone; D000432:Methanol