Serum-free hybridoma culture: ethical, scientific and safety considerations.
Despite considerable progress in the development of cell culture techniques, including the development of the serum- and protein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum (FBS) supplemented media are still commonly used: a practice that raises ethical, scientific and safety concerns. The use of FBS in hybridoma culture media is examined here, with regards to the development and production of monoclonal antibodies (mAbs), and it is our recommendation that researchers adopt serum-free cell culture methods to reduce animal use in this area.
Even
Megha S
MS
Physicians Committee for Responsible Medicine, 5100 Wisconsin Ave NW, Suite 400, Washington, DC 20016, USA. meven@pcrm.org
Sandusky
Chad B
CB
Barnard
Neal D
ND
D023401:Animal Use Alternatives / Q000941:ethics / Q000639:trends; D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000941:ethics* / Q000379:methods; D016895:Culture Media, Serum-Free; D006801:Humans; D006825:Hybridomas; D016272:Occupational Health
D016895:Culture Media, Serum-Free