New latent fluorophore for DT diaphorase.
[reaction: see text] This study describes the design and synthesis of a novel latent fluorophore 3 for DT diaphorase based on the trimethyl lock effect and characterization of its enzymatic kinetics. Fluorophore 3 is also a sensitive fluorimetric reagent for detecting glucose when coupled with DTD and glucose dehydrogenase.
Huang
Sheng-Tung
ST
College of Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taiwan, ROC. ws75624@ntut.edu.tw
Lin
Yuh-Ling
YL
D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D005947:Glucose / Q000032:analysis; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism; D015394:Molecular Structure; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000032:analysis* / Q000378:metabolism; D012235:Rhodamines / Q000737:chemistry
D005456:Fluorescent Dyes; D012235:Rhodamines; D042843:Glucose 1-Dehydrogenase; D016660:NAD(P)H Dehydrogenase (Quinone); D005947:Glucose