title: Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta).
abstract: To preserve genetic variability and minimize genetic subdivision among captive Macaca mulatta at each of the U.S. National Institutes of Health (NIH)-sponsored regional research colonies, the genetic structure of each colony must be characterized. To compare population genetic and demographic parameters across colonies and generations, one standard panel of highly informative genetic markers is required. We assembled a core marker set of four multiplex polymerase chain reaction (PCR) panels comprising 15 autosomal short tandem repeat (STR) loci with high information content selected from existing panels of well-characterized markers that are currently used for parentage assessment and genetic management of rhesus macaques. We then assessed the effectiveness of these loci for providing high probabilities of individual identification and parentage resolution, and for estimating population genetic parameters that are useful for genetic management.
authors.affiliation: Veterinary Genetics Laboratory, School of Veterinary Medicine, California National Primate Research Center, University of California-Davis, California 95616, USA. skanthaswamy@ucdavis.edu
authors.forename: Sreetharan
authors.initials: S
authors.lastname: Kanthaswamy
authors.forename: Andrea
authors.initials: A
authors.lastname: von Dollen
authors.forename: Jennifer D
authors.initials: JD
authors.lastname: Kurushima
authors.forename: Ona
authors.initials: O
authors.lastname: Alminas
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Rogers
authors.forename: Betsy
authors.initials: B
authors.lastname: Ferguson
authors.forename: Nicholas W
authors.initials: NW
authors.lastname: Lerche
authors.forename: Philip C
authors.initials: PC
authors.lastname: Allen
authors.forename: David Glenn
authors.initials: DG
authors.lastname: Smith
mesh_terms: D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D001947:Breeding / Q000379:methods; D017931:DNA Primers; D019075:Electrophoresis, Capillary / Q000379:methods / Q000662:veterinary; D005787:Gene Frequency / Q000235:genetics; D014644:Genetic Variation; D006579:Heterozygote; D008253:Macaca mulatta / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards / Q000662:veterinary; D011110:Polymorphism, Genetic; D013047:Specific Pathogen-Free Organisms
chemical_list: D017931:DNA Primers