Cre reporter system to monitor the translocation of type III secreted proteins into host cells.
Central to the study of type III secretion systems is the availability of reporter systems to monitor bacterial protein translocation into host cells. We report here the development of a bacteriophage P1 Cre recombinase-based system to monitor the translocation of bacterial proteins into mammalian cells. Bacteriophage P1 Cre recombinase fused to the secretion and translocation signals of Salmonella enterica serovar Typhimurium of the type III secreted protein SopE was secreted in a type III secretion system-dependent fashion. More importantly, the SopE-Cre chimera was translocated into host cells via the type III secretion system and activated the expression of luciferase and green fluorescent protein reporters of Cre recombinase activity.
Briones
Gabriel
G
Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536, USA.
Hofreuter
Dirk
D
Galán
Jorge E
JE
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D017101:Bacteriophage P1 / Q000201:enzymology* / Q000235:genetics; D019556:COS Cells; D002522:Chlorocebus aethiops; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010957:Plasmids; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; C103987:SopE protein, Salmonella; C419942:Spi1 protein, Salmonella; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D008156:Luciferases; C045073:Cre recombinase; D019426:Integrases