Activation of protein kinase C enhances 1-methyl-4-phenylpyridinium ion (MPP+)-induced hydroxylradical generation in rat striatum.
The present study examined the effect of chelerlythrine, a protein kinase C (PKC) inhibitor, on 1-methyl-4-phenylpyridine (MPP+)-induced hydroxyl radicals (*OH) in rat striatum. Rats were anesthetized, and sodium salicylate (0.5 mM or 0.5 nmol/microl/min) was infused through a microdialysis probe to detect the *OH generation as reflected by the non-enzymatic formation of 2,3-dihydroxybenzoic acid (2,3-DHBA) in the striatum. Dopamine (DA)-selective neurotoxin, MPP+, infusion into the striatum of rats induces *OH formation, trapped as 2,3-DHBA. The application of chelerythrine, a potent and selective protein kinase C (PKC) inhibitor, suppressed MPP+ -induced *OH formation. The results in the present study suggests the protective effect of chelerythrine on *OH generation induced by MPP+.
Obata
Toshio
T
Department of Analytical Chemistry, Ohu University School of Pharmaceutical Sciences, Koriyama, Fukushima 963-8611, Japan. t-obata@pha.ohu-u.ac.jp
D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology*; D000470:Alkaloids; D000818:Animals; D053119:Benzophenanthridines; D002396:Catechols / Q000378:metabolism; D002414:Cations, Monovalent; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004789:Enzyme Activation; D062385:Hydroxybenzoates; D017665:Hydroxyl Radical / Q000378:metabolism*; D008297:Male; D010617:Phenanthridines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000470:Alkaloids; D053119:Benzophenanthridines; D002396:Catechols; D002414:Cations, Monovalent; D062385:Hydroxybenzoates; D010617:Phenanthridines; D017665:Hydroxyl Radical; C009135:2,3-dihydroxybenzoic acid; C016299:chelerythrine; D011493:Protein Kinase C; D015655:1-Methyl-4-phenylpyridinium