Interfacial behavior of fatty-acylated sericin prepared by lipase-catalyzed solid-phase synthesis.
Fatty-acylated sericin {1:0.7 molar ratio of sericin (Mr 18,700) to oleic acid} was prepared by lipase-catalyzed solid-phase synthesis in n-hexane containing oleic acid to endow sericin with interfacial properties. Acylation with oleic acid was confirmed by 1H-NMR. The fatty-acylated sericin exhibited superior emulsifying activity index and emulsion stability in the presence of 0-0.5 M NaCl, in a temperature range of 30-80 degrees C and pH range of 2-7, as compared with the control sericin. The fatty-acylated sericin (1:0.4 molar ratio) prepared by using low-molecular-weight sericin (Mr 5,000) also exhibited superior emulsifying properties. The affinity of the fatty-acylated sericin to a hydrophobic surface as evaluated by a biomolecular interaction analyzer was about twice as much as that of the control sericin. The fatty-acylated sericin showed retarded water vaporization, similar to the control sericin, indicating good retention of moistness, and was adsorbed four times as much to defatted wool with little desorption as compared with the control sericin.
Ogino
Masato
M
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Tanaka
Rie
R
Hattori
Makoto
M
Yoshida
Tadashi
T
Yokote
Yoshiko
Y
Takahashi
Koji
K
D000215:Acylation; D000818:Animals; D012831:Bombyx / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002384:Catalysis; D004655:Emulsions / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010316:Particle Size; D017433:Protein Structure, Secondary; D047030:Sericins / Q000737:chemistry* / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014867:Water; D014935:Wool / Q000737:chemistry
D004655:Emulsions; D004800:Enzymes, Immobilized; D005227:Fatty Acids; D047030:Sericins; D014867:Water; D012965:Sodium Chloride; D008049:Lipase