[Inhibition of HCV replication by HCV specific U1 small nuclear RNA chimeric ribozyme in vivo].
OBJECTIVE
To determine the advantage of U1 small nuclear RNA as a ribozyme vector (U1-Rz) to inhibit HCV replication in vivo.


METHODS
The 3rd stem-loop was substituted by HCV core specific ribozyme to construct an U1-Rz eucaryotic expression plasmid. Then it was co-transfected with pCMV/T7-NCRC Delta-luc into Huh7 cell line mediated by lipofectin. The cell lysis supernatant was subjected to Western blot and lumenometer to determine the luciferase levels.


RESULTS
A U1 snRNA chimeric ribozyme was constructed successfully. Both Rz and U1-Rz inhibited luciferase expression in Huh7 by 48.64% and 87.46%, respectively.


CONCLUSIONS
Rz has more efficacy in cells when using U1 snRNA delivery system. U1 can be an efficient vector for HCV specific ribozyme.
Wang
Mei-xia
MX
Department of Infectious Diseases, First Hospital of Jilin University, Changchun 130021, China. wangmeixiad@163.com
Jin
Qing-long
QL
Pan
Yu
Y
Wang
Feng
F
Niu
Jun-qi
JQ
D001483:Base Sequence; D015316:Genetic Therapy; D005822:Genetic Vectors; D016174:Hepacivirus / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D016337:RNA, Catalytic / Q000235:genetics*; D012342:RNA, Small Nuclear / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics*
D016337:RNA, Catalytic; D012342:RNA, Small Nuclear; C405483:U1 small nuclear RNA; C427204:hammerhead ribozyme