Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta).
To preserve genetic variability and minimize genetic subdivision among captive Macaca mulatta at each of the U.S. National Institutes of Health (NIH)-sponsored regional research colonies, the genetic structure of each colony must be characterized. To compare population genetic and demographic parameters across colonies and generations, one standard panel of highly informative genetic markers is required. We assembled a core marker set of four multiplex polymerase chain reaction (PCR) panels comprising 15 autosomal short tandem repeat (STR) loci with high information content selected from existing panels of well-characterized markers that are currently used for parentage assessment and genetic management of rhesus macaques. We then assessed the effectiveness of these loci for providing high probabilities of individual identification and parentage resolution, and for estimating population genetic parameters that are useful for genetic management.
Kanthaswamy
Sreetharan
S
Veterinary Genetics Laboratory, School of Veterinary Medicine, California National Primate Research Center, University of California-Davis, California 95616, USA. skanthaswamy@ucdavis.edu
von Dollen
Andrea
A
Kurushima
Jennifer D
JD
Alminas
Ona
O
Rogers
Jeffrey
J
Ferguson
Betsy
B
Lerche
Nicholas W
NW
Allen
Philip C
PC
Smith
David Glenn
DG
D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D001947:Breeding / Q000379:methods; D017931:DNA Primers; D019075:Electrophoresis, Capillary / Q000379:methods / Q000662:veterinary; D005787:Gene Frequency / Q000235:genetics; D014644:Genetic Variation; D006579:Heterozygote; D008253:Macaca mulatta / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards / Q000662:veterinary; D011110:Polymorphism, Genetic; D013047:Specific Pathogen-Free Organisms
D017931:DNA Primers