title: Total hydroxyoctadecadienoic acid as a marker for lipid peroxidation in vivo.
abstract: An improved method for the measurement of lipid peroxidation in vivo has been recently developed, where total hydroxyoctadecadienoic acid (HODE) and 7-hydroxycholesterol (FCOH) were determined by GC/MS analysis from physiological samples after reduction with sodium borohydride and saponification by potassium hydroxide. In this method, both free and ester forms of hydroperoxides and ketones as well as hydroxides of linoleic acid and cholesterol are measured as HODE and FCOH, respectively. The ratio of stereo-isomer, (Z, E)-HODE/(E, E)-HODE, could be also measured. In the present study, in order to examine the effect of continuous, slow flux of free radicals in vivo, a water-soluble radical generator was administered to rats and mice and the amounts of HODE and 8-isoprostane in plasma and liver were measured. It was found that the administration of free radical-generating azo compound increased the level of HODE and decreased the (Z, E)-HODE/(E, E)-HODE ratio in both plasma and liver. The level of HODE was much higher than 8-isoprostane.
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Human Stress Signal Research Center, Ikeda 563-8577, Japan. yoshida-ya@aist.go.jp
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Mieko
authors.initials: M
authors.lastname: Hayakawa
authors.forename: Etsuo
authors.initials: E
authors.lastname: Niki
mesh_terms: D000578:Amidines; D000818:Animals; D001391:Azo Compounds / Q000008:administration & dosage; D015415:Biomarkers / Q000032:analysis* / Q000097:blood; D015237:Dinoprost / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood; D004326:Drinking; D005231:Fatty Acids, Unsaturated / Q000032:analysis* / Q000097:blood; D005609:Free Radicals / Q000008:administration & dosage; D008401:Gas Chromatography-Mass Spectrometry; D006888:Hydroxycholesterols / Q000032:analysis / Q000097:blood; D007093:Imidazoles / Q000008:administration & dosage; D015227:Lipid Peroxidation; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D051381:Rats; D013047:Specific Pathogen-Free Organisms
chemical_list: D000578:Amidines; D001391:Azo Compounds; D015415:Biomarkers; D005231:Fatty Acids, Unsaturated; D005609:Free Radicals; D006888:Hydroxycholesterols; D007093:Imidazoles; C430118:VA 061; C495366:hydroxyoctadecadienoic acid; C075750:8-epi-prostaglandin F2alpha; C046728:2,2'-azobis(2-amidinopropane); D015237:Dinoprost