A molecular framework for plant regeneration.
Plants and some animals have a profound capacity to regenerate organs from adult tissues. Molecular mechanisms for regeneration have, however, been largely unexplored. Here we investigate a local regeneration response in Arabidopsis roots. Laser-induced wounding disrupts the flow of auxin-a cell-fate-instructive plant hormone-in root tips, and we demonstrate that resulting cell-fate changes require the PLETHORA, SHORTROOT, and SCARECROW transcription factors. These transcription factors regulate the expression and polar position of PIN auxin efflux-facilitating membrane proteins to reconstitute auxin transport in renewed root tips. Thus, a regeneration mechanism using embryonic root stem-cell patterning factors first responds to and subsequently stabilizes a new hormone distribution.
Xu
Jian
J
Department of Molecular Genetics, Utrecht University, Padualaan 8, 3584CH Utrecht, Netherlands.
Hofhuis
Hugo
H
Heidstra
Renze
R
Sauer
Michael
M
Friml
Jirí
J
Scheres
Ben
B
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008954:Models, Biological; D010937:Plant Growth Regulators / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012038:Regeneration; D013234:Stem Cells / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D010937:Plant Growth Regulators; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C116363:PIN1 protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis; C451260:PIN4 protein, Arabidopsis; C493246:PLT1 protein, Arabidopsis; C100978:SCR protein, Arabidopsis; C410510:SHORT ROOT protein, Arabidopsis