title: Lack of effect of metyrapone and exogenous cortisol on early porcine conceptus development.
abstract: A study was conducted to evaluate the influence of maternal cortisol on early conceptus development in pigs (Sus scrofa). The corticosteroid synthesis inhibitor metyrapone was injected daily during days 14-19 of pregnancy, without (n = 6) and with commensurate administration of cortisol (n = 6). Blood samples were taken via an indwelling jugular catheter on days 14 and 18, and conceptuses were harvested during surgery on day 20. Compared with vehicle-injected control dams (n = 7) plasma cortisol and aldosterone concentrations were decreased (P < 0.01) by 52 and 29%, respectively, by metyrapone treatment. Cortisol administration reversed decreases in plasma cortisol by day 18. There were no treatment-associated effects on conceptus survival or size. Nor were there treatment-associated effects on allantoic fluid volume or content. Trophodermal glucocorticoid receptor (GR) mRNA expression decreased by 34% (P < 0.05) in metyrapone-treated pigs, and was not further influenced by concomitant administration of cortisol, thereby suggesting an influence of aldosterone on GR mRNA expression. Also, when all pigs were considered, there were treatment-independent second-order polynomial regressions (P < 0.05) between maternal plasma cortisol concentrations and embryonic weight, allantoic size and allantoic glucose concentrations, and between plasma aldosterone concentrations and trophodermal GR mRNA expression. Such biphasic corticosteroid concentration versus tissue parameter curves are noteworthy, but difficult to interpret validly. They may suggest that an appropriate corticosteroid environment is necessary for optimal porcine embryonic development during this stage of gestation, but cannot overshadow the absence of treatment effects on the porcine embryonic measures evaluated.
authors.affiliation: USDA-ARS, Roman L. Hruska US Meat Animal Research Center, Clay Center, NE 68933, USA. harold.klemcke@amedd.army.mil
authors.forename: H G
authors.initials: HG
authors.lastname: Klemcke
authors.forename: J L
authors.initials: JL
authors.lastname: Vallet
authors.forename: R K
authors.initials: RK
authors.lastname: Christenson
mesh_terms: D000450:Aldosterone / Q000097:blood; D000818:Animals; D004338:Drug Combinations; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology; D005260:Female; D006854:Hydrocortisone / Q000008:administration & dosage* / Q000097:blood*; D008431:Maternal-Fetal Exchange / Q000187:drug effects / Q000502:physiology*; D008797:Metyrapone / Q000008:administration & dosage*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000097:blood*; D013552:Swine / Q000097:blood* / Q000196:embryology*
chemical_list: D004338:Drug Combinations; D000450:Aldosterone; D006854:Hydrocortisone; D008797:Metyrapone