Selective extraction of derivates of p-hydroxy-benzoic acid from plant material by using a molecularly imprinted polymer.
Selective SPE of derivates of p-hydroxybenzoic acid (pHBA) from plant extract of Melissa officinalis is presented using a molecularly imprinted polymer (MIP) made with protocatechuic acid (PA) as template molecule. MIP was prepared with acrylamide as functional monomer, ethylene glycol dimethacrylate as crosslinking monomer and ACN as porogen. MIP was evaluated towards six phenolic acids: PA, gallic acid, pHBA, vanillic acid (VA), gentisic acid (GeA) and syringic acid (SyrA), and then steps of molecularly imprinted SPE (MISPE) procedure were optimized. The best specific binding capacity of MIP was obtained for PA in ACN (34.7 microg/g of MIP). Other tested acids were also bound on MIP if they were dissolved in this solvent. ACN was chosen as solvent for sample application. M. officinalis was extracted into methanol/water (4:1, v/v), the extract was then evaporated to dryness and dissolved in ACN before application on MIP. Water and ACN were used as washing solvents and elution of benzoic acids was performed by means of a mixture methanol/acetic acid (9:1, v/v). pHBA, GA, PA and VA were extracted with recoveries of 56.3-82.1% using this MISPE method. GeA was not determined in plant extract.
Karasová
Gabriela
G
Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republic.
Lehotay
Jozef
J
Sádecká
Jana
J
Skacáni
Ivan
I
Lachová
Miroslava
M
D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D062385:Hydroxybenzoates / Q000737:chemistry; D031334:Melissa / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D010226:Parabens / Q000032:analysis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis*; D010944:Plants / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry*; D012997:Solvents / Q000494:pharmacology
D000097:Acetonitriles; D062385:Hydroxybenzoates; D010226:Parabens; D010936:Plant Extracts; D011108:Polymers; D012997:Solvents; C017616:phenolic acid; C038193:4-hydroxybenzoic acid; D019342:Acetic Acid; D000432:Methanol; C032159:acetonitrile