title: Molecular detection of Paracoccidioides brasiliensis in soil.
abstract: The objective of the present study was to evaluate different techniques for the detection of Paracoccidioides brasiliensis in soil, e.g., culture, animal inoculation and specific DNA amplification by Nested PCR. We designed species-specific inner primers derived from rDNA regions (ITS, 5.8S gene) and found their sensitivity to be higher than culture and animal inoculation. In addition, the sensitivity of these primers was higher than p27-gene primers developed for detection of P. brasiliensis in soil in a previous study. DNA from P. brasiliensis was detected in soil artificially seeded with the fungus (positive soil control) and from environmental samples collected in an armadillo burrow.
authors.affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, Botucatu, SP Brasil.
authors.forename: Raquel Cordeiro
authors.initials: RC
authors.lastname: Theodoro
authors.forename: João Manuel Grisi
authors.initials: JM
authors.lastname: Candeias
authors.forename: João Pessoa
authors.initials: JP
authors.lastname: Araújo
authors.forename: Sandra De Moraes Gimenes
authors.initials: Sde M
authors.lastname: Bosco
authors.forename: Severino Assis Da Graça
authors.initials: SA
authors.lastname: Macoris
authors.forename: Luis Otavio Junior
authors.initials: LO
authors.lastname: Padula
authors.forename: Marcello
authors.initials: M
authors.lastname: Franco
authors.forename: Eduardo
authors.initials: E
authors.lastname: Bagagli
mesh_terms: D001938:Brazil; D004271:DNA, Fungal / Q000032:analysis*; D021901:DNA, Intergenic / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D009172:Mycology / Q000379:methods; D010228:Paracoccidioides / Q000254:growth & development / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D012680:Sensitivity and Specificity; D012988:Soil Microbiology
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S