title: Canine adenovirus type 2 infection in four puppies with neurological signs.
abstract: Four nine- to 11-week-old puppies developed respiratory and neurological signs due to an infection with canine adenovirus type 2 (cav-2); three of these were euthanased. They had moderate, diffuse pneumonia but there were no histological abnormalities in the central nervous system. Adenovirus-specific nucleic acid was detected by pcr in samples of lung and brain and the amplified product was 99.8 per cent homologous with the cav-2 reference strain Toronto a26/61. The positive pcr result was confirmed by in situ hybridisation in samples of lung, liver and spleen, but not in brain, and cav was isolated in cell culture from lung material; pcrs for canine distemper virus and canine herpesvirus-specific nucleic acids were negative, but large amounts of Bordetella bronchiseptica were isolated from lung material.
authors.affiliation: Clinical Virology Group, Institute of Virology, University of Veterinary Medicine, A-1210 Vienna, Austria.
authors.forename: V
authors.initials: V
authors.lastname: Benetka
authors.forename: H
authors.initials: H
authors.lastname: Weissenböck
authors.forename: I
authors.initials: I
authors.lastname: Kudielka
authors.forename: C
authors.initials: C
authors.lastname: Pallan
authors.forename: G
authors.initials: G
authors.lastname: Rothmüller
authors.forename: K
authors.initials: K
authors.lastname: Möstl
mesh_terms: D006516:Adenoviruses, Canine / Q000302:isolation & purification*; D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D017809:Fatal Outcome; D006522:Hepatitis, Infectious Canine / Q000175:diagnosis* / Q000473:pathology; D017403:In Situ Hybridization / Q000662:veterinary; D008099:Liver / Q000821:virology; D008168:Lung / Q000821:virology; D011024:Pneumonia, Viral / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D013154:Spleen / Q000821:virology