title: Sonic hedgehog promotes mouse inner ear progenitor cell proliferation and hair cell generation in vitro.
abstract: Sonic hedgehog (Shh) signaling is essential for auditory cell fate determination and inner ear dorsal/ventral patterning during development. Here, we show that Shh accelerates inner ear progenitor cell proliferation, and the inhibitor of Shh signaling cyclopamine reduces mitotic growth of otocyst cells in vitro. The number of hair cells in cultures of inner ear progenitor cells that were treated with Shh was significantly higher than that in control cultures. When Shh signaling was blocked with cyclopamine, hair cell generation was largely inhibited. Our results suggest that Shh may be a regulator of inner ear progenitor cell growth and hair cell generation.
authors.affiliation: Otology Skull Base Surgery Department, Hearing Research Institute, PR China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhengmin
authors.initials: Z
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Yunzheng
authors.initials: Y
authors.lastname: Shen
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Stefan
authors.initials: S
authors.lastname: Heller
authors.forename: Huawei
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D007758:Ear, Inner / Q000166:cytology*; D004622:Embryo, Mammalian; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000502:physiology*; D053823:Hedgehog Proteins; D007150:Immunohistochemistry / Q000379:methods; D066298:In Vitro Techniques; D007211:Indoles; D051379:Mice; D008810:Mice, Inbred C57BL; D024507:Nonmuscle Myosin Type IIA / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology*; D015534:Trans-Activators / Q000494:pharmacology / Q000502:physiology*; D014704:Veratrum Alkaloids / Q000494:pharmacology
chemical_list: D053823:Hedgehog Proteins; D007211:Indoles; C465570:Shh protein, mouse; D015534:Trans-Activators; D014704:Veratrum Alkaloids; C007293:DAPI; D024507:Nonmuscle Myosin Type IIA; D001973:Bromodeoxyuridine; C000541:cyclopamine