title: Is protein overlap in two-dimensional gels a serious practical problem?
abstract: In a recently published article, Campostrini et al. [Proteomics 2005, 5, 2385-2395] raised questions regarding the utility of 2-D gels in proteomics research. We believe that the authors have overlooked several key issues including the dynamic range of protein expression and the sensitivity of the analytical methods used to explore a proteome. We argue that 2-D gels have and will continue to provide meaningful quantitative data when applied to proteomic analysis and that the practical significance of spot overlap has been overstated.
authors.affiliation: Section of Pulmonary Medicine, Department of Pediatrics, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA.
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Hunsucker
authors.forename: Mark W
authors.initials: MW
authors.lastname: Duncan
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D011506:Proteins / Q000032:analysis*; D040901:Proteomics
chemical_list: D011506:Proteins