title: High-throughput assay for small molecules that modulate zebrafish embryonic heart rate.
abstract: To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.
authors.affiliation: Developmental Biology Laboratory, Cardiovascular Research Center, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts 02129, USA. gburns@cvrc.mgh.harvard.edu
authors.forename: C Geoffrey
authors.initials: CG
authors.lastname: Burns
authors.forename: David J
authors.initials: DJ
authors.lastname: Milan
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Grande
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Rottbauer
authors.forename: Calum A
authors.initials: CA
authors.lastname: MacRae
authors.forename: Mark C
authors.initials: MC
authors.lastname: Fishman
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002317:Cardiovascular Agents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004625:Embryo, Nonmammalian / Q000502:physiology; D005240:Feasibility Studies; D049452:Green Fluorescent Proteins / Q000032:analysis*; D006321:Heart / Q000187:drug effects* / Q000196:embryology / Q000502:physiology*; D006339:Heart Rate / Q000187:drug effects* / Q000502:physiology; D009206:Myocardium / Q000378:metabolism; D013997:Time Factors; D015027:Zebrafish
chemical_list: D002317:Cardiovascular Agents; D049452:Green Fluorescent Proteins