title: A new method of wet scanning electron microscopy for the analysis of myelination in EAE mouse model of multiple sclerosis.
abstract: Development of effective therapies for multiple sclerosis (MS) is dependent on the advancement of improved tools for evaluation of progression of this disease in animal models. We present a novel technique utilizing scanning electron microscopy (SEM) for imaging wet biological specimens thus enabling rapid and high-resolution imaging of myelin in mouse spinal cord (SC). We demonstrate the advantages of using the wet SEM technique to image myelin in a murine model of MS, experimental autoimmune encephalomyelitis (EAE) induced in the Biozzi (antibody-high) mouse, by sensitization with spinal cord homogenate (SCH) in adjuvant. Our studies show that the methodology allows easy identification of normal and pathological components with great clarity, which is then correlated with light microscopy (LM) and validated thereby. Furthermore, we demonstrate gold immunolabeling of specific epitopes. We conclude that the new technique provides a quick, accurate, and detailed structural evaluation of the SC that can be applied to advance the research of MS.
authors.affiliation: Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health, Research Triangle Park, NC, USA. anyska@bezeqint.net
authors.forename: Abraham
authors.initials: A
authors.lastname: Nyska
authors.forename: Miri
authors.initials: M
authors.lastname: Horowitz
authors.forename: Debbie
authors.initials: D
authors.lastname: Anaby
authors.forename: Alon
authors.initials: A
authors.lastname: Sabban
authors.forename: Ilit
authors.initials: I
authors.lastname: Leizerman
authors.forename: Eran
authors.initials: E
authors.lastname: Blaugrund
authors.forename: Adi
authors.initials: A
authors.lastname: Mayk
authors.forename: Vered
authors.initials: V
authors.lastname: Behar
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000473:pathology*; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009103:Multiple Sclerosis / Q000473:pathology*; D009186:Myelin Sheath / Q000648:ultrastructure*; D015203:Reproducibility of Results; D013116:Spinal Cord / Q000648:ultrastructure*