title: [Molecular biological survey of tick-born arboviruses in southern part of Xinjiang].
abstract: BACKGROUND
  To disclose the species and distribution of tick-borne arboviruses in the southern part of Xinjiang.
  
  
  METHODS
  Totally 5045 ticks were collected from 36 collecting sites of 23 places in the southern Xinjiang, which were made into cDNA pools with pd(N)6 primer through RT-PCR method. Then PCR was used to detect viral nucleotide sequence from cDNA.
  
  
  RESULTS
  All 34 cDNAs showed negative to flavivirus and California serogroup virus primers; but nairovirus and primers derived from Xinjiang hemorrhagic fever virus had amplified and yielded some obvious bands corresponding to the nucleotide sequences of Xinjiang hemorrhagic fever virus. A phylogenetic analysis was done to the obtained partial sequences of L and S segments.
  
  
  CONCLUSIONS
  Nucleotide sequences of Neither flaviviruses nor California serogroup viruses were detected from the samples. However partial L segment sequence was first reported in China. Phylogenetic analysis of partial L and S segments disclosed the molecular characteristic of Xinjiang hemorrhagic fever virus.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Xin-jun
authors.initials: XJ
authors.lastname: Lv
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
authors.forename: Yu-ming
authors.initials: YM
authors.lastname: Feng
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhi
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Xu
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Shi-hong
authors.initials: SH
authors.lastname: Fu
authors.forename: Huan-yu
authors.initials: HY
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017282:Tick-Borne Diseases / Q000821:virology; D013987:Ticks / Q000821:virology*