Breakdown products on metabolic pathway of degradation of benz[a]anthracene by a ligninolytic fungus.
Cultures of the ligninolytic fungus Irpex lacteus incubated in a nutrient liquid medium degraded more than 70% of the initially applied benz[a]anthracene within 14 days. At the first step of metabolization, benz[a]anthracene was transformed via a typical pathway of ligninolytic fungi to benz[a]anthracene-7,12-dione (BaAQ). The product was further transformed by at least two ways, whereas one is complied with the anthracene metabolic pathway of I. lacteus. Benz[a]anthracene-7,12-dione was degraded to 1,2-naphthalenedicarboxylic acid and phthalic acid that was followed with production of 2-hydroxymethyl benzoic acid or monomethyl and dimethylesters of phthalic acid. Another degradation product of BaAQ was identified as 1-tetralone. Its transformation via 1,4-naphthalenedione, 1,4-naphthalenediol and 1,2,3,4-tetrahydro-1-hydroxynaphthalene resulted again in phthalic acid. None of the intermediates were identified as dead-end metabolites. Metabolites produced by ring cleavage of benz[a]anthracene using the ligninolytic fungus are firstly presented in this work.
Cajthaml
Tomás
T
Division of Ecology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, CZ-142 20 Prague 4, Czech Republic. cajthaml@biomed.cas.cz
Erbanová
Pavla
P
Sasek
Václav
V
Moeder
Monika
M
D001487:Basidiomycota / Q000254:growth & development*; D001551:Benz(a)Anthracenes / Q000032:analysis*; D001673:Biodegradation, Environmental; D015394:Molecular Structure; D010084:Oxidation-Reduction; D012989:Soil Pollutants / Q000032:analysis*
D001551:Benz(a)Anthracenes; D012989:Soil Pollutants; C030935:benz(a)anthracene