title: Phosphorylation of tyrosine 1248-ERBB2 measured by chemiluminescence-linked immunoassay is an independent predictor of poor prognosis in primary breast cancer patients.
abstract: ERBB2 (HER2/Neu) gene amplification and overexpression is associated with increased risk of metastases and shorter survival in breast cancer. Tyrosine 1248 is a major phosphorylation site of ERBB2 and reflects the activation status of the receptor. The aim of this study was to investigate the relationships between quantitative levels of pY1248-ERBB2 (p-ERBB2) and the expression of epidermal growth factor receptor (EGFR)-family members, and whether p-ERBB2 could provide additional prognostic value compared with established prognostic markers. For this purpose we developed a highly sensitive chemiluminescence-linked immunoassay (CLISA) and detected p-ERBB2 levels in 70 primary breast cancer biopsies. Phosphorylated ERBB2 correlated with EGFR and ERBB2, and inversely with oestrogen receptor (ER), progesterone receptor (PgR) and ERBB4 expression levels. Additionally, p-ERBB2 was associated with poor clinical outcome in univariate and multivariate Cox regression analysis. Further studies are needed to evaluate the predictive value of p-ERBB2.
authors.affiliation: Stiftung Tumorbank Basel, Lörracherstrasse 50, CH-4125 Riehen, Switzerland.
authors.forename: Jonas
authors.initials: J
authors.lastname: Cicenas
authors.forename: Patrick
authors.initials: P
authors.lastname: Urban
authors.forename: Willy
authors.initials: W
authors.lastname: Küng
authors.forename: Vincent
authors.initials: V
authors.lastname: Vuaroqueaux
authors.forename: Martin
authors.initials: M
authors.lastname: Labuhn
authors.forename: Edward
authors.initials: E
authors.lastname: Wight
authors.forename: Urs
authors.initials: U
authors.lastname: Eppenberger
authors.forename: Serenella
authors.initials: S
authors.lastname: Eppenberger-Castori
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements / Q000379:methods; D010766:Phosphorylation; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; D014443:Tyrosine