title: Diverse biomodulatory effects of glucomannan from Candida utilis.
abstract: Using four experimental model systems, it was demonstrated that glucomannan (GM) isolated from the cell wall of the industrial yeast Candida utilis revealed a broad range of protective activities. This effect depended on the nature and mode of action of the counteracting genotoxic compound as well as on the experimental model system used. In the Saccharomyces bioprotectivity assay, GM increased resistance towards ofloxacin-induced toxicity in the wild type and recombination repair-deficient yeast strains significantly enhancing survival of the cells. In the chromosomal aberration assay, GM exerted anticlastogenic effect against maleic hydrazide induced clastogenicity in Vicia faba L. In the DNA-topology assay, GM protected plasmid DNA from the breaks induced by Fe(2+) ions, but enhanced damage induced by bleomycin and hydrogen peroxide. In the cell-revitalization assay, it enhanced cytotoxic/cytostatic effect of teniposide applied to mouse leukemia cells. Thus, depending on the experimental model, GM acted as antimutagen, anticlastogen, DNA breaks inhibitor or inducer, and as cytotoxic/cytostatic effect enhancer. Several possible mechanisms of bioprotective action underlying the observed activities are suggested including iron chelation and free radical scavenging. The results imply that GM is a polysaccharide with marked biological activities and suggest its potential biomedical application, especially in combination with other bioactive compounds.
authors.affiliation: Department of Genetics, Faculty of Sciences, Comenius University, 84215 Bratislava, Slovakia.
authors.forename: Eva
authors.initials: E
authors.lastname: Miadoková
authors.forename: Sona
authors.initials: S
authors.lastname: Svidová
authors.forename: Viera
authors.initials: V
authors.lastname: Vlcková
authors.forename: Viola
authors.initials: V
authors.lastname: Dúhová
authors.forename: Slavomíra
authors.initials: S
authors.lastname: Nad'ová
authors.forename: Peter
authors.initials: P
authors.lastname: Rauko
authors.forename: Grigorij
authors.initials: G
authors.lastname: Kogan
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000494:pharmacology; D002175:Candida / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D004249:DNA Damage; D007941:Leukemia P388 / Q000473:pathology; D008351:Mannans / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D012441:Saccharomyces cerevisiae / Q000187:drug effects
chemical_list: D008351:Mannans; D001761:Bleomycin; C022901:(1-6)-alpha-glucomannan