Gene expression profiles in acute myeloid leukemia with common translocations using SAGE.
Identification of the specific cytogenetic abnormality is one of the critical steps for classification of acute myeloblastic leukemia (AML) which influences the selection of appropriate therapy and provides information about disease prognosis. However at present, the genetic complexity of AML is only partially understood. To obtain a comprehensive, unbiased, quantitative measure, we performed serial analysis of gene expression (SAGE) on CD15(+) myeloid progenitor cells from 22 AML patients who had four of the most common translocations, namely t(8;21), t(15;17), t(9;11), and inv(16). The quantitative data provide clear evidence that the major change in all these translocation-carrying leukemias is a decrease in expression of the majority of transcripts compared with normal CD15(+) cells. From a total of 1,247,535 SAGE tags, we identified 2,604 transcripts whose expression was significantly altered in these leukemias compared with normal myeloid progenitor cells. The gene ontology of the 1,110 transcripts that matched known genes revealed that each translocation had a uniquely altered profile in various functional categories including regulation of transcription, cell cycle, protein synthesis, and apoptosis. Our global analysis of gene expression of common translocations in AML can focus attention on the function of the genes with altered expression for future biological studies as well as highlight genes/pathways for more specifically targeted therapy.
Lee
Sanggyu
S
Department of Medicine, University of Chicago, IL 60637, USA.
Chen
Jianjun
J
Zhou
Guolin
G
Shi
Run Zhang
RZ
Bouffard
Gerard G
GG
Kocherginsky
Masha
M
Ge
Xijin
X
Sun
Miao
M
Jayathilaka
Nimanthi
N
Kim
Yeong Cheol
YC
Emmanuel
Neelmini
N
Bohlander
Stefan K
SK
Minden
Mark
M
Kline
Justin
J
Ozer
Ozden
O
Larson
Richard A
RA
LeBeau
Michelle M
MM
Green
Eric D
ED
Trent
Jeffery
J
Karrison
Theodore
T
Liu
Piu Paul
PP
Wang
San Ming
SM
Rowley
Janet D
JD
D017209:Apoptosis; D002454:Cell Differentiation; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D019295:Computational Biology; D018076:DNA, Complementary / Q000378:metabolism; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D016256:Lewis X Antigen / Q000096:biosynthesis; D023461:Myeloid Progenitor Cells / Q000166:cytology; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014178:Translocation, Genetic
D018076:DNA, Complementary; D016256:Lewis X Antigen; D012333:RNA, Messenger; D012313:RNA