[Observations on the use of TESA blot for the serological diagnosis of Chagas' disease].
TESA blot was compared with indirect hemagglutination, indirect immunofluorescence and ELISA tests. In sera from 30 participants infected with Trypanosoma cruzi, and in 30 non infected the four techniques produced entirely equivalent results, all positive and all negative, respectively. In cases admitted to be inconclusive or in visceral leishmaniasis, frequent false positives were detected. However, TESA blot contributed with the least proportion of them.
Amato Neto
Vicente
V
Laboratório de Investigação Médica-Parasitologia, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil. amatonet@usp.br
De Marchi
Cláudia Regina
CR
Ferreira
Cláudio Santos
CS
Ferreira
Antonio Walter
AW
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology; D014355:Chagas Disease / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D011237:Predictive Value of Tests; D014349:Trypanosoma cruzi / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan