title: Molecular characterization of the capsule locus from non-typeable Staphylococcus aureus.
abstract: Most Staphylococcus aureus express a serotype 5 or 8 capsular polysaccharide (CP). However, 20-25% of human isolates and up to 86% of bovine strains of S. aureus are non-typeable (NT), i.e. non-reactive with antibodies to CP types 1, 2, 5 or 8. A vaccine that targets the S. aureus CP would not protect against NT strains. The aim of this study was to characterize NT S. aureus isolates at the molecular level to explain their lack of type 5 or 8 capsule production. The cap5(8) locus was present in all 22 NT clinical isolates from humans, eight of 21 bovine isolates, and in all eight sequenced strains. NT strains positive for the cap5(8) transcript had mutations within essential capsule genes and could be complemented in trans. S. aureus strains with reduced cap5(8) transcript had mutations within the cap5A promoter, decreased RNAIII levels, or a truncated arlR gene product. More than one mutation was identified in several isolates. The cap5(8) locus was replaced by IS257 in 13 of 21 NT bovine isolates of S. aureus. Lack of capsule expression in NT S. aureus can be explained by multiple mechanisms, and the data argue against the existence of capsule serotypes other than 1, 2, 5 and 8.
authors.affiliation: Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors.forename: Jordan L
authors.initials: JL
authors.lastname: Cocchiaro
authors.forename: Marisa I
authors.initials: MI
authors.lastname: Gomez
authors.forename: Allison
authors.initials: A
authors.lastname: Risley
authors.forename: Robert
authors.initials: R
authors.lastname: Solinga
authors.forename: Daniel O
authors.initials: DO
authors.lastname: Sordelli
authors.forename: Jean C
authors.initials: JC
authors.lastname: Lee
mesh_terms: D000818:Animals; D016667:Bacterial Capsules / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002417:Cattle; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D011135:Polysaccharides, Bacterial / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: C411334:ArlR protein, Staphylococcus aureus; D001426:Bacterial Proteins; C107803:Cap5 protein, Staphylococcus; D011135:Polysaccharides, Bacterial; C121798:pneumococcal polysaccharide, type 8