title: Microstructured liposome array.
abstract: Conversion of a DNA chip to a nanocapsule array was performed by grafting on a liposome an oligonucleotide complementary to an oligonucleotide bound to the array. Each liposome may be loaded by a soluble molecule or may present a hydrophobic or amphiphilic molecule inserted in its wall. To detect liposomes on the chip, we used fluorescent dyes encapsulated in the liposome internal volume or fluorescent lipids. We observed that an oligonucleotide-grafted liposome containing a defined dye specifically accumulated on the area where its complementary oligonucleotide had been spotted on the array. The virtually unlimited amount of addresses allows the specific binding of large amounts of liposomes in one single batch.
authors.affiliation: Institut de Pharmacologie et de Biologie Structurale, 205, route de Narbonne 31077 Toulouse Cedex, France.
authors.forename: Barnabé
authors.initials: B
authors.lastname: Chaize
authors.forename: Michel
authors.initials: M
authors.lastname: Nguyen
authors.forename: Tristan
authors.initials: T
authors.lastname: Ruysschaert
authors.forename: Véronique
authors.initials: V
authors.lastname: le Berre
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Trévisiol
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Caminade
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Majoral
authors.forename: Geneviève
authors.initials: G
authors.lastname: Pratviel
authors.forename: Bernard
authors.initials: B
authors.lastname: Meunier
authors.forename: Mathias
authors.initials: M
authors.lastname: Winterhalter
authors.forename: Didier
authors.initials: D
authors.lastname: Fournier
mesh_terms: D008081:Liposomes / Q000737:chemistry*; D036103:Nanotechnology; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D008081:Liposomes; D009841:Oligonucleotides