title: The effects of N-acetylcysteine on antioxidant enzyme activities in experimental testicular torsion.
abstract: Testicular torsion is a serious problem in male children and, if not treated at the right time, can lead to subfertility and infertility. The main reason for testicular damage is ischemia-reperfusion injury. A number of chemical substances have been used to protect testes against ischemia-reperfusion injury in experimental animals. The possible protective effect of N-acetylcysteine on testicular tissue after testicular detorsion was examined in the current study. Twenty-four rats were divided into four groups: sham operation, torsion, detorsion, and NAC + detorsion groups (n = 6 for each group). Excluding sham operation group, the rats were subjected to unilateral torsion (720-degree rotation in clockwise direction). After torsion (5 h) and detorsion (2 h), unilateral orchidectomy was performed. Malondialdehyde levels and superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activities were determined in testicular tissue. Administration of N-acetylcysteine caused a decrease in malondialdehyde levels and an increase in glutathione peroxidase levels compared to detorsion group. The results suggest that N-acetylcysteine may be a potential protective agent for preventing the negative biochemical changes related to oxidative stress in testicular injury caused by testis torsion.
authors.affiliation: Department of Pediatric Surgery, Karadeinz Technical Unversity, Trabzon, Turkey. dralicay@yahoo.com
authors.forename: Ali
authors.initials: A
authors.lastname: Cay
authors.forename: Ahmet
authors.initials: A
authors.lastname: Alver
authors.forename: Murat
authors.initials: M
authors.lastname: Küçük
authors.forename: Osman
authors.initials: O
authors.lastname: Işik
authors.forename: M Selçuk
authors.initials: MS
authors.lastname: Eminağaoğlu
authors.forename: S Caner
authors.initials: SC
authors.lastname: Karahan
authors.forename: Orhan
authors.initials: O
authors.lastname: Değer
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D013733:Testicular Diseases / Q000150:complications*; D014102:Torsion Abnormality / Q000150:complications
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D000111:Acetylcysteine