Synthesis and characterization of a head-tail type polycation block copolymer as a nonviral gene vector.
A head-tail type polycation block copolymer, which is composed of the polyamidoamine (PAMAM) dendron and poly(L-lysine) (PLL) blocks, was newly designed as a nonviral gene vector in this study. This block copolymer (PAMAM dendron-PLL) was successfully synthesized in two steps: the synthesis of the PAMAM dendron block and the polymerization of the PLL block from the PAMAM dendron block. PAMAM dendron and PLL blocks in block copolymer showed independent deprotonation behavior, and their pK(a) were determined to be 6.8 and 9.0, respectively. The complexation with pDNA was evaluated by gel retardation assay and dye exclusion assay, and both assays indicated that pDNA was selectively complexed with PLL block of block copolymer. Also, the PAMAM dendron-PLL poplyplexes showed 10(2) fold higher transfection efficiency to HeLa cells as that for PLL polyplexes. This might be due to the buffering effect of the PAMAM dendron block. This block copolymer could produce a function share in each block, i.e., tail block complexed with pDNA and head block showed a buffering effect. This molecular design of the head-tail type block copolymer might provide a new approach for realizing in vivo gene therapy.
Harada
Atsushi
A
Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan. harada@chem.osakafu-u.ac.jp
Kawamura
Masanori
M
Matsuo
Takashi
T
Takahashi
Toshinari
T
Kono
Kenji
K
D004247:DNA / Q000008:administration & dosage / Q000378:metabolism; D050091:Dendrimers; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000138:chemical synthesis*; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011073:Polyamines / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis*; D014162:Transfection / Q000379:methods*
D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines; D011108:Polymers; D011107:Polylysine; D004247:DNA; D008156:Luciferases