title: Sensitive column-switching high-performance liquid chromatography method for determination of propiverine in human plasma.
abstract: A sensitive column-switching high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection was developed for the determination of propiverine in human plasma. Propiverine and internal standard, oxybutynin, were extracted from human plasma that had been made basic with 5N sodium hydroxide into methyl tert-butyl ether. The extracted plasma sample was injected onto the HPLC system consisting of a pretreatment column, a concentrating column, and an analytical column, which were connected with a six-port switching valve. The assay was linear in concentration ranges of 2-200 ng/ml for propiverine in human plasma. This method showed excellent sensitivity (a limit of detection of 0.5 ng/ml), good precision and accuracy. This method is suitable for bioequivalence studies following single dose in healthy volunteers.
authors.affiliation: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Eunmi
authors.initials: E
authors.lastname: Ban
authors.forename: Jeong-Eun
authors.initials: JE
authors.lastname: Maeng
authors.forename: Jong Soo
authors.initials: JS
authors.lastname: Woo
authors.forename: Chong-Kook
authors.initials: CK
authors.lastname: Kim
mesh_terms: D001561:Benzilates / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001561:Benzilates; C015586:propiverine