title: Patterns of latex allergen recognition in children sensitized to natural rubber latex.
abstract: Single recombinant latex allergens permit the study of the pattern of sensitization to individual allergens. We aimed to quantify the IgE-response to individual latex allergens in children sensitized to latex. The study group included 31 latex-sensitized children: 26 operated at least twice, 20 of them with spina bifida; two children with one operation and three atopic non-operated children. IgE antibodies to rHev b 1, rHev b 3, rHev b 5, rHev b 6.01, rHev b 7.02 and rHev b 8, coupled to ImmunoCAPs, were measured in each serum. IgE responses to rHev b 1, rHev b 5 and rHev b 6.01 were found in 17 children each, and their mean +/- s.d. levels were 5 +/- 7.4, 16.8 +/- 14 and 10 +/- 18 kU/l, respectively. IgE responses to rHev b 3 (4 +/- 5.4 kU/l) were found in eight children. Two children had IgE to rHev b 7 (1.7 and 3.2 kU/l), and none to rHev b 8. Four sera were negative to all tested recombinant allergens. We divided the patients in three groups: sensitized only to rHev b 1, sensitized only to rHev b 5 and/or rHev b 6.01, and sensitized to both rHev b 1 and to rHev b 5 and/or rHev b 6.01. The three groups had the same profile of clinical features. Hev b 5 induces the quantitatively higher IgE responses in children with multiple surgeries sensitized to latex. Responses to Hev b 6.01 equal those of Hev b 1.
authors.affiliation: Pediatric Allergy Unit, Children's Hospital La Fe, Av. Campanar 21, 46009 Valencia, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Pamies
authors.forename: Fernando
authors.initials: F
authors.lastname: Oliver
authors.forename: Monika
authors.initials: M
authors.lastname: Raulf-Heimsoth
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Rihs
authors.forename: Domingo
authors.initials: D
authors.lastname: Barber
authors.forename: Manuel
authors.initials: M
authors.lastname: Boquete
authors.forename: Antonio
authors.initials: A
authors.lastname: Nieto
authors.forename: Angel
authors.initials: A
authors.lastname: Mazón
mesh_terms: D000293:Adolescent; D000485:Allergens / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007223:Infant; D020315:Latex Hypersensitivity / Q000276:immunology*; D008297:Male; D012408:Rubber; D016135:Spinal Dysraphism / Q000276:immunology
chemical_list: D000485:Allergens; D007073:Immunoglobulin E; D012408:Rubber