Actin content and actin polymerization in hepatoma Morris 5123 tumor bearing rats after treatment with cysteine protease inhibitor and vitamin E.
OBJECTIVE
Actin microfilaments are components of cytoskeleton, the structure involved in many cellular functions, including cell motility, and morphology, its necessary for tumor progression and metastasis. We investigated the effects of vitamin E and human placetal cysteine protease inhibitor (CPI) on actin content and polymerization after implantation the hepatoma Morris 5123 tumor in Buffalo rat.


METHODS
We measured the size and survival animals treated with human placental cysteine protease inhibitor (CPI) plus vitamin E and none treated rats. Also measured the actin content and polymerization in the tumor and liver tissues by the inhibition of DNase I from bovine pancreas under standard assay conditions


RESULTS
We observed that the combination 10 mg of vitamin E plus 200 microg CPI obtained the best results than authors. In those cases the animals survived for longer than 4 weeks. In numerous cases the 70 % of tumors disappeared following CPI and vitamin E application. The number of complete tumor responses was higher after combination 10 mg of vitamin E plus CPI 200 microg i.e. 5/7 rats than others group. We showed statistically significant decrease of monomeric (G), filamentous (F) and total actin as well as the F:G ratio level in tumor tissues after the rats was treated with CPI plus vitamin E in comparison with control animals.


CONCLUSIONS
The new therapy may be the way for therapeutic intervention, aimed at stopping and possibly reversing the process of metastatic growth, with the use of drugs affecting actin polymerization.
Sebzda
Tadeusz
T
Department of Pathophysiology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Saleh
Yousif
Y
Malicka-Blaszkiewicz
Maria
M
Nowak
Dorota
D
Siewinski
Maciej
M
Ziolkowski
Piotr
P
Kopec
Wieslaw
W
D000199:Actins / Q000378:metabolism*; D000818:Animals; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D009368:Neoplasm Transplantation; D010920:Placenta / Q000201:enzymology; D011108:Polymers; D011247:Pregnancy; D051381:Rats; D011915:Rats, Inbred BUF; D014810:Vitamin E / Q000494:pharmacology*; D014815:Vitamins / Q000494:pharmacology*
D000199:Actins; D015853:Cysteine Proteinase Inhibitors; D011108:Polymers; D014815:Vitamins; D014810:Vitamin E