title: Characterization of Methylophaga sp. strain SK1 cytochrome cL expressed in Escherichia coli.
abstract: Methylophaga sp. strain SK1 is a new restricted facultative methanol-oxidizing bacterium that was isolated from seawater. The aim of this study was to characterize the electron carriers involved in the methanol oxidation process in Methylophaga sp. strain SK1. The gene encoding cytochrome c(L) (mxaG) was cloned and the recombinant gene was expressed in Escherichia coli DH5 under strict anaerobic conditions. The recombinant cytochrome c(L) had the same molecular weight and absorption spectra as the wild-type cytochrome c(L) both in the reduced and oxidized forms. The electron flow rate from methanol dehydrogenase (MDH) to the recombinant cytochrome c(L) was similar to that from MDH to the wild-type cytochrome c(L). These results suggest that recombinant cytochrome c(L) acts as a physiological primary electron acceptor for MDH.
authors.affiliation: Department of Biomaterials Engineering, Chosum University, Gwangju, Republic of Korea.
authors.forename: Hee Gon
authors.initials: HG
authors.lastname: Kim
authors.forename: Trong Nhat
authors.initials: TN
authors.lastname: Phan
authors.forename: Tae Sa
authors.initials: TS
authors.lastname: Jang
authors.forename: Moonjoo
authors.initials: M
authors.lastname: Koh
authors.forename: Si Wouk
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000693:Anaerobiosis; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008970:Molecular Weight; D044147:Piscirickettsiaceae / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012623:Seawater / Q000382:microbiology; D013057:Spectrum Analysis; D014871:Water Microbiology
chemical_list: D003574:Cytochrome c Group; D011994:Recombinant Proteins; C058498:cytochrome C(L); D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)