title: The effect of machine perfusion on the arteries of porcine kidneys.
abstract: OBJECTIVE
  Machine perfusion is an excellent method of assessing the viability of a kidney graft and can also potentially improve the quality of an equivocal kidney. Several authors have expressed concerns that machine perfusion can potentially damage the vessels of the kidney but until now, no studies have been performed to clarify this issue. We aimed to examine the effect of machine perfusion on the renal arteries of porcine kidneys.
  
  
  METHODS
  Eight pairs of kidneys were removed from pigs in the abattoir. One kidney of each pair was preserved on ice for 24 hours. The other kidney from the same animal was initially stored on ice until arrival at the laboratory when it was perfused on the RM3 machine for 4 hours and then stored again on ice for the remainder of the 24 hours. After 24 hours, since the retrieval and initial storage on ice at the abattoir, tissue samples were obtained from all renal arteries at 3 different sites. These samples were sent for histologic evaluation.
  
  
  RESULTS
  Machine perfusion caused more damage at a statistically significant level compared with simple cold storage only for the first sample site, which was the part of the renal artery closest to the perfusion cannula.
  
  
  CONCLUSIONS
  Our experiments suggest that machine perfusion, even when it is done lege artis, can damage the part of the renal artery closest to the adaptor, which can potentially result in a higher incidence of posttransplant arterial thrombosis. Therefore, excision of the first part of the renal artery should be considered prior to transplantation, and modifications of the perfusion technique must be developed to minimize damage to the renal arteries.
authors.affiliation: The West London Renal and Transplant Centre, Hammersmith Hospital, London, and Imperial College of Science, Technology and Medicine, University of London, UK. Inge.Peerlinck@cancer.org.uk
authors.forename: Inge D L
authors.initials: ID
authors.lastname: Peerlinck
authors.forename: Alexander
authors.initials: A
authors.lastname: Barlas
authors.forename: Andreas
authors.initials: A
authors.lastname: Karameris
authors.forename: Vassilios E
authors.initials: VE
authors.lastname: Papalois
mesh_terms: D000818:Animals; D015925:Cryopreservation; D007668:Kidney / Q000098:blood supply*; D016030:Kidney Transplantation; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000009:adverse effects*; D012077:Renal Artery / Q000293:injuries*; D013552:Swine