title: Calcium-regulated type III secretion of Yop proteins by an Escherichia coli hha mutant carrying a Yersinia pestis pCD1 virulence plasmid.
abstract: A series of four large deletions that removed a total of ca. 36 kb of DNA from the ca. 70-kb Yersinia pestis pCD1 virulence plasmid were constructed using lambda Red-mediated recombination. Escherichia coli hha deletion mutants carrying the virulence plasmid with the deletions expressed a functional calcium-regulated type III secretion system. The E. coli hha/pCD1 system should facilitate molecular studies of the type III secretion process.
authors.affiliation: Department of Microbiology and Immunology, University of Miami Miller School of Medicine, P.O. Box 016960 (R-138), Miami, FL 33101, USA.
authors.forename: Sara Schesser
authors.initials: SS
authors.lastname: Bartra
authors.forename: Michael W
authors.initials: MW
authors.lastname: Jackson
authors.forename: Julia A
authors.initials: JA
authors.lastname: Ross
authors.forename: Gregory V
authors.initials: GV
authors.lastname: Plano
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005821:Genetic Techniques; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D014774:Virulence; D015010:Yersinia pestis / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C079209:hha protein, E coli; D002118:Calcium