title: A laser flash-induced kinetic analysis of in vivo photosystem I reduction by site-directed mutants of plastocyanin and cytochrome c6 in Synechocystis sp. PCC 6803.
abstract: In cyanobacteria, plastocyanin and cytochrome c6 are two soluble metalloproteins which can alternately serve as electron donors to photosystem I. From site-directed mutagenesis studies in vitro, it is well-established that both hydrophobic and electrostatic forces are involved in the interaction between the donor proteins and photosystem I. Hence, two isofunctional areas, a hydrophobic one in the north and an acidic one in the east, have been described on the surface of both electron donors. In this work, we have tested the relevance of such kinds of interactions in the photosystem I reduction inside the cell. Several plastocyanin and cytochrome c6 site-directed mutant strains affecting both the acidic and hydrophobic regions of the two metalloproteins, which were previously characterized in vitro, have been constructed. The photosystem I reduction kinetics of the different mutants have been analyzed by laser flash absorption spectroscopy. Relevant differences have been found between the in vitro and in vivo results, mainly regarding the role played by the electrostatic interactions. Adding positive electrostatic charges to the acidic patch of plastocyanin and cytochrome c6 promotes an enhanced interaction with photosystem I in vitro but yields the opposite effect in vivo. These discrepancies are discussed in view of the different environmental conditions, in vitro and in vivo, for the reaction mechanism of photosystem I reduction, namely, differential interaction of the electron donors with the thylakoidal membrane and kinetics of donor exchange.
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Universidad de Sevilla y Consejo Superior de Investigaciones Científicas, Sevilla, Spain.
authors.forename: Raúl V
authors.initials: RV
authors.lastname: Durán
authors.forename: Manuel
authors.initials: M
authors.lastname: Hervás
authors.forename: Berta
authors.initials: B
authors.lastname: De la Cerda
authors.forename: Miguel A
authors.initials: MA
authors.lastname: De la Rosa
authors.forename: José A
authors.initials: JA
authors.lastname: Navarro
mesh_terms: D045365:Cytochromes c6 / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D007834:Lasers; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010084:Oxidation-Reduction / Q000528:radiation effects; D045331:Photosystem I Protein Complex / Q000378:metabolism* / Q000528:radiation effects*; D010970:Plastocyanin / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D046939:Synechocystis / Q000201:enzymology* / Q000528:radiation effects; D013816:Thermodynamics
chemical_list: D045365:Cytochromes c6; D045331:Photosystem I Protein Complex; D010970:Plastocyanin