Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information on a hyperthermophilic archaeon, Sulfolobus tokodaii strain 7.
sn-Glycerol-1-phosphate dehydrogenase is responsible for the formation of sn-glycerol-1-phosphate, the backbone of membrane phospholipids of Archaea. This activity had never been detected in cell-free extract of Sulfolobus sp. Here we report the detection of this activity on the thermostable ST0344 protein of Sulfolobus tokodaii expressed in Escherichia coli, which was predicted from genomic information on S. tokodaii. This is another line of evidence for the general mechanism of sn-glycerol-1-phosphate formation by the enzyme.
Koga
Yosuke
Y
Department of Chemistry, School of Medicine, University of Occupational and Environmental Health, Japan. kogay@med.uoeh-u.ac.jp
Ohga
Mami
M
Tsujimura
Masanari
M
Morii
Hiroyuki
H
Kawarabayasi
Yutaka
Y
D000595:Amino Acid Sequence; D017124:Conserved Sequence; D004795:Enzyme Stability; D015870:Gene Expression; D020745:Genome, Archaeal / Q000235:genetics*; D005993:Glycerolphosphate Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D016944:Sulfolobus / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D013696:Temperature
D005994:Glycerophosphates; D011994:Recombinant Proteins; D009249:NADP; D005993:Glycerolphosphate Dehydrogenase; C098215:glycerol-1-phosphate dehydrogenase