Genetics of symbiosis in Lotus japonicus: recombinant inbred lines, comparative genetic maps, and map position of 35 symbiotic loci.
Development of molecular tools for the analysis of the plant genetic contribution to rhizobial and mycorrhizal symbiosis has provided major advances in our understanding of plant-microbe interactions, and several key symbiotic genes have been identified and characterized. In order to increase the efficiency of genetic analysis in the model legume Lotus japonicus, we present here a selection of improved genetic tools. The two genetic linkage maps previously developed from an interspecific cross between L. japonicus Gifu and L. filicaulis, and an intraspecific cross between the two ecotypes L. japonicus Gifu and L. japonicus MG-20, were aligned through a set of anchor markers. Regions of linkage groups, where genetic resolution is obtained preferentially using one or the other parental combination, are highlighted. Additional genetic resolution and stabilized mapping populations were obtained in recombinant inbred lines derived by a single seed descent from the two populations. For faster mapping of new loci, a selection of reliable markers spread over the chromosome arms provides a common framework for more efficient identification of new alleles and new symbiotic loci among uncharacterized mutant lines. Combining resources from the Lotus community, map positions of a large collection of symbiotic loci are provided together with alleles and closely linked molecular markers. Altogether, this establishes a common genetic resource for Lotus spp. A web-based version will enable this resource to be curated and updated regularly.
Sandal
Niels
N
Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej, Aarhus C, Denmark. nns@mb.au.dk
Petersen
Thomas Rørby
TR
Murray
Jeremy
J
Umehara
Yosuke
Y
Karas
Bogumil
B
Yano
Koji
K
Kumagai
Hirotaka
H
Yoshikawa
Makoto
M
Saito
Katsuharu
K
Hayashi
Masaki
M
Murakami
Yasuhiro
Y
Wang
Xinwang
X
Hakoyama
Tsuneo
T
Imaizumi-Anraku
Haruko
H
Sato
Shusei
S
Kato
Tomohiko
T
Chen
Wenli
W
Hossain
Md Shakhawat
MS
Shibata
Satoshi
S
Wang
Trevor L
TL
Yokota
Keisuke
K
Larsen
Knud
K
Kanamori
Norihito
N
Madsen
Esben
E
Radutoiu
Simona
S
Madsen
Lene H
LH
Radu
Talida Gratiela
TG
Krusell
Lene
L
Ooki
Yasuhiro
Y
Banba
Mari
M
Betti
Marco
M
Rispail
Nicolas
N
Skøt
Leif
L
Tuck
Elaine
E
Perry
Jillian
J
Yoshida
Satoko
S
Vickers
Kate
K
Pike
Jodie
J
Mulder
Lonneke
L
Charpentier
Myriam
M
Müller
Judith
J
Ohtomo
Ryo
R
Kojima
Tomoko
T
Ando
Shotaro
S
Marquez
Antonio J
AJ
Gresshoff
Peter M
PM
Harada
Kyuya
K
Webb
Judith
J
Hata
Shingo
S
Suganuma
Norio
N
Kouchi
Hiroshi
H
Kawasaki
Shinji
S
Tabata
Satoshi
S
Hayashi
Makoto
M
Parniske
Martin
M
Szczyglowski
Krzysztof
K
Kawaguchi
Masayoshi
M
Stougaard
Jens
J
D000483:Alleles; D002874:Chromosome Mapping; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D000070116:Lotus / Q000235:genetics*; D018895:Microsatellite Repeats; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D011995:Recombination, Genetic; D013559:Symbiosis / Q000235:genetics*
D005819:Genetic Markers