Respiratory nitrate and nitrite pathway in the denitrifier haloarchaeon Haloferax mediterranei.
Haloferax mediterranei cells are able to use high nitrate or nitrite concentrations as electron acceptors under anoxic conditions. The nar operon, which has eight open reading frames, has been sequenced and its regulation has been characterized at the transcriptional level. The narG and narH genes encode the Nar (respiratory nitrate reductase) catalytic subunit (NarG) and the electron transfer Nar subunit (NarH) respectively. Nar has been purified and characterized in vitro. This characterization has included protein-film voltammetry and preliminary EPR studies.
Martínez-Espinosa
R M
RM
División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Campus de San Vicente del Raspeig, 03080 Alicante, Spain.
Richardson
D J
DJ
Butt
J N
JN
Bonete
M J
MJ
D019069:Cell Respiration / Q000502:physiology*; D004563:Electrochemistry; D004583:Electrons; D019623:Haloferax mediterranei / Q000235:genetics / Q000378:metabolism*; D050901:Nitrate Reductase / Q000235:genetics / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D009876:Operon; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism
D009566:Nitrates; D009573:Nitrites; D021122:Protein Subunits; D050901:Nitrate Reductase