title: Polymorphism of beta-lactoglobulin gene in Indian goats and its effect on milk yield.
abstract: Polymorphism in the beta-LG gene in the Indian goat was investigated by the SDS-PAGE and PCR-RFLP method. SDS-PAGE was carried out in 1098 samples belonging to 8 different breeds of Indian goats. The electrophoretic pattern in the beta-LG locus showed the presence of AA and AB genotypes with frequencies of 0.81 and 0.19, 0.89 and 0.11, 0.50 and 0.50, 0.80 and 0.20, 0.84 and 0.16, 1.00 and 0.00, 0.98 and 0.02 and 0.950 and 0.050 in Jamunapari, Barbari, Marwari, Sirohi, Jakhrana, Beetal, Local UP and Local MP goats, respectively. A total of 358 individuals belonging to 13 different genetic groups were analyzed by the PCR-RFLP method. The amplified product was observed as 426 bp and the restriction digestion with SacII revealed three genotypes, namely S1S1, S1S2 and S2S2 at the beta-LG locus. The frequency of the S2S2 genotype ranged from 0.42 to 1.00 in the population. An analysis was carried out in Jamunapari and Barbari goats to observe the effect of the beta-LG genotype on 90-day milk yield. Least squares analysis of data showed that beta-LG AA animals had higher milk yield than the beta-LG AB genotype in both breeds (P < 0.01).
authors.affiliation: Division of Genetics and Breeding, CIRG, Makhdoom, Farah, Mathura-281122, UP, India.
authors.forename: Ajay
authors.initials: A
authors.lastname: Kumar
authors.forename: Pramod K
authors.initials: PK
authors.lastname: Rout
authors.forename: Ramadhar
authors.initials: R
authors.lastname: Roy
mesh_terms: D000818:Animals; D018900:Dinucleotide Repeats / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D007194:India; D007774:Lactation / Q000235:genetics*; D007782:Lactoglobulins / Q000235:genetics*; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D007782:Lactoglobulins; D008894:Milk Proteins