title: Neural network-based analysis of thiol proteomics data in identifying potential selenium targets.
abstract: Generation of a monomethylated selenium metabolite is critical for the anticancer activity of selenium. Because of its strong nucleophilicity, the metabolite can react directly with protein thiols to cause redox modification. Here, we report a neural network-based analysis to identify potential selenium targets. A reactive thiol specific reagent, BIAM, was used to monitor thiol proteome changes on 2D gel. We constructed a dynamic model and evaluated the relative importance of proteins mediating the cellular responses to selenium. Information from this study will provide new clues to unravel mechanisms of anticancer action of selenium. High impact selenium targets could also serve as biomarkers to gauge the efficacy of selenium chemoprevention.
authors.affiliation: School of Computer Science & Engineering, Inha University, Korea.
authors.forename: Jong-Sik
authors.initials: JS
authors.lastname: Lee
authors.forename: Yong-Beom
authors.initials: YB
authors.lastname: Ma
authors.forename: Kyoung-Soo
authors.initials: KS
authors.lastname: Choi
authors.forename: Soo-Yeon
authors.initials: SY
authors.lastname: Park
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Baek
authors.forename: Young-Mee
authors.initials: YM
authors.lastname: Park
authors.forename: Ke
authors.initials: K
authors.lastname: Zu
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhang
authors.forename: Clement
authors.initials: C
authors.lastname: Ip
authors.forename: Yeul Hong
authors.initials: YH
authors.lastname: Kim
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Park
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D005029:Ethylenediamines / Q000737:chemistry; D006801:Humans; D008297:Male; D016571:Neural Networks, Computer; D016566:Organoselenium Compounds / Q000378:metabolism / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012643:Selenium / Q000378:metabolism / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D005029:Ethylenediamines; C093128:N-biotinoyl-N'-iodoacetylethylenediamine; D016566:Organoselenium Compounds; D011506:Proteins; D020543:Proteome; D013438:Sulfhydryl Compounds; D001710:Biotin; C008493:methylselenic acid; D012643:Selenium