title: A recombinant Yellow Fever 17D vaccine expressing Lassa virus glycoproteins.
abstract: The Yellow Fever Vaccine 17D (YFV17D) has been used as a vector for the Lassa virus glycoprotein precursor (LASV-GPC) resulting in construction of YFV17D/LASV-GPC recombinant virus. The virus was replication-competent and processed the LASV-GPC in cell cultures. The recombinant replicated poorly in guinea pigs but still elicited specific antibodies against LASV and YFV17D antigens. A single subcutaneous injection of the recombinant vaccine protected strain 13 guinea pigs against fatal Lassa Fever. This study demonstrates the potential to develop an YFV17D-based bivalent vaccine against two viruses that are endemic in the same area of Africa.
authors.affiliation: Department of Medical Microbiology, Center for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Bredenbeek
authors.forename: Richard
authors.initials: R
authors.lastname: Molenkamp
authors.forename: Willy J M
authors.initials: WJ
authors.lastname: Spaan
authors.forename: Vincent
authors.initials: V
authors.lastname: Deubel
authors.forename: Phillippe
authors.initials: P
authors.lastname: Marianneau
authors.forename: Maria S
authors.initials: MS
authors.lastname: Salvato
authors.forename: Dmitry
authors.initials: D
authors.lastname: Moshkoff
authors.forename: Juan
authors.initials: J
authors.lastname: Zapata
authors.forename: Ilia
authors.initials: I
authors.lastname: Tikhonov
authors.forename: Jean
authors.initials: J
authors.lastname: Patterson
authors.forename: Ricardo
authors.initials: R
authors.lastname: Carrion
authors.forename: Anysha
authors.initials: A
authors.lastname: Ticer
authors.forename: Kathleen
authors.initials: K
authors.lastname: Brasky
authors.forename: Igor S
authors.initials: IS
authors.lastname: Lukashevich
mesh_terms: D000818:Animals; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D007835:Lassa Fever / Q000517:prevention & control*; D007836:Lassa virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D015004:Yellow Fever / Q000517:prevention & control; D022341:Yellow Fever Vaccine / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D015005:Yellow fever virus / Q000276:immunology
chemical_list: D006023:Glycoproteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D022341:Yellow Fever Vaccine