Mutation in the Sp1 motif of the bovine leptin gene affects its expression.
Leptin is expressed mainly by adipocytes and plays a crucial role in the regulation of energy expenditure, food intake, and adiposity. Using PCR-heteroduplex analysis and sequencing, we investigated a C/G substitution in the promoter region of the bovine leptin gene. Application of the electrophoretic mobility shift assay showed that the C-->G transversion decreased the leptin gene promoter binding capacity for nuclear proteins. With real-time PCR and Western blotting, we showed that the leptin expression level was higher in cattle with the CC than with the GG genotype.
Adamowicz
Tatiana
T
Department of Genetics and Animal Breeding, Agricultural University of Poznan, Poznan, Poland.
Flisikowski
Krzysztof
K
Starzyński
Rafał
R
Zwierzchowski
Lech
L
Switoński
Marek
M
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D015342:DNA Probes; D005786:Gene Expression Regulation / Q000235:genetics*; D020738:Leptin / Q000235:genetics*; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor / Q000235:genetics*
D017931:DNA Primers; D015342:DNA Probes; D020738:Leptin; D016329:Sp1 Transcription Factor