Fabrication and application of neoglycolipid arrays in a microtiter plate.
The work presented herein is a new noncovalent glycoarray assembly method for microplates created by simply mixing together a carbohydrate and a tetradecylamine. Alpha-mannose was utilized in the model study and product formation was detected by lectin binding. The method can be further extended to array complex carbohydrates.
Huang
Gang-Liang
GL
School of Life Science, Shandong University, Jinan City, Jinan 250100, China. hgl226@126.com
Zhang
Hou-Cheng
HC
Wang
Peng-George
PG
D002240:Carbohydrate Sequence; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate; D006017:Glycolipids / Q000737:chemistry*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D008969:Molecular Sequence Data
D006017:Glycolipids; D007075:Immunoglobulin M; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate