title: Structure of DraD invasin from uropathogenic Escherichia coli: a dimer with swapped beta-tails.
abstract: The dra gene cluster of uropathogenic strains of Escherichia coli produces proteins involved in bacterial attachment to and invasion of the eukaryotic host tissues. The crystal structure of a construct of E. coli DraD possessing an additional C-terminal extension of 13 amino acids, including a His6 tag, has been solved at a resolution of 1.05 angstroms. The protein forms symmetric dimers through the exchange of the C-terminal beta-strands, which participate in the immunoglobulin-like beta-sandwich fold of each subunit. This structure confirms that DraD is able to act as an acceptor in the donor-strand complementation mechanism of fiber formation but, in contrast to DraE adhesin, its native sequence does not have a donor strand; therefore, DraD can only be located at the tip of the fiber.
authors.affiliation: Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Jedrzejczak
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Dauter
authors.forename: Mirosława
authors.initials: M
authors.lastname: Dauter
authors.forename: Rafał
authors.initials: R
authors.lastname: Piatek
authors.forename: Beata
authors.initials: B
authors.lastname: Zalewska
authors.forename: Marta
authors.initials: M
authors.lastname: Mróz
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Bury
authors.forename: Bogdan
authors.initials: B
authors.lastname: Nowicki
authors.forename: Józef
authors.initials: J
authors.lastname: Kur
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry*; D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D019281:Dimerization; D004926:Escherichia coli / Q000737:chemistry* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D005816:Genetic Complementation Test; D006639:Histidine / Q000737:chemistry; D007136:Immunoglobulins / Q000737:chemistry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry; D011487:Protein Conformation
chemical_list: D018830:Adhesins, Escherichia coli; D029968:Escherichia coli Proteins; C471213:His-His-His-His-His-His; D007136:Immunoglobulins; D009842:Oligopeptides; D006639:Histidine