title: Packaging of gold particles in viral capsids.
abstract: In-vitro self-assembly conditions known to result in generating infectious virions have been used in vitro to reassemble bromovirus capsid proteins around negatively charged gold nanoparticles cores. We discuss here the optical properties (elastic light scattering) and the influence of the core size and of the functional moiety on the resulting virus-like particles. Our results indicate that the formation of a closed shell, as opposed to an amorphous protein coat, does occur and that the shell/core interactions can be tuned using different coatings on the nanoparticle core. Such studies may lead to real-time monitoring of viral traffic on the scale of a single virus, as well as to the possibility of chemical sensing along the intracellular and intercellular viral pathways and contribute to a better understanding of the virus transport and cellular compartmentalization.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Eun-Soo
authors.initials: ES
authors.lastname: Kwak
authors.forename: Barry
authors.initials: B
authors.lastname: Stein
authors.forename: C Cheng
authors.initials: CC
authors.lastname: Kao
authors.forename: Bogdan
authors.initials: B
authors.lastname: Dragnea
mesh_terms: D017795:Bromovirus / Q000737:chemistry* / Q000648:ultrastructure; D002213:Capsid / Q000737:chemistry* / Q000648:ultrastructure; D006046:Gold; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology
chemical_list: D006046:Gold