Differential proteomic serum pattern of low molecular weight proteins expressed by adenocarcinoma lung cancer patients.
Based on the assumption that proteins can emanate from tumour to serum, we investigated whether serum low molecular weight proteins (LMW) can discriminate lung cancer patients from healthy donors. Pooled sera from 20 lung cancer patients matched in sex (men), histological type (adenocarcinoma) and stage (IIIB and IV) and from 20 healthy donors (men) were submitted to 2-DE coupled to MALDI-TOF peptide mass fingerprinting. Results of 2D-E/ MALDI-TOF showed five up regulated proteins (immunoglobulin lambda chain, transthyretin monomer, haptoglobin-alfa 2 and two isoforms of serum amyloid protein) and one down regulated (fragment of apolipoprotein A-I) in patients. All differentially expressed proteins, except immunoglobulin, may be acting as a non-specific sign of inflammation in cancer and transthyretin monomer may act as a possible blood marker to CSF barrier disruption that occurs, e.g., in cerebral metastasis. In conclusion, this work shows the usefulness of 2D-gel electrophoresis and mass spectrometry proteomic techniques for the identification of up- and down-regulated proteins in serum from adenocarcinoma lung cancer patients.
Maciel
Carla Mendes
CM
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco C, Ilha do Fundão, CEP: 21941-900, Rio de Janeiro-RJ, Brazil.
Junqueira
Magno
M
Paschoal
Marcos Eduardo Machado
ME
Kawamura
Marcia Tie
MT
Duarte
Ricardo Luiz Menezes
RL
Carvalho
Maria da Glória da Costa
Mda G
Domont
Gilberto Barbosa
GB
D000230:Adenocarcinoma / Q000378:metabolism*; D000328:Adult; D001798:Blood Proteins / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006868:Hydrolysis; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D008970:Molecular Weight; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000737:chemistry
D001798:Blood Proteins; D014357:Trypsin