Wound repair and proliferation of bronchial epithelial cells enhanced by bombesin receptor subtype 3 activation.
The present study was designed to investigate the role of bombesin receptor subtype 3 (BRS-3) in airway wound repair. The results showed that: (1) There was few expression of BRS-3 mRNA in the control group. In contrast, the expression of BRS-3 mRNA was gradually increased in the early 2 days, and peaked on the fourth day, and then decreased in the ozone-stressed AHR animal. BRS-3 mRNA was distributed in the ciliated columnar epithelium, monolayer columnar epithelium cells, scattered mesenchymal cells and Type II alveolar cells; (2) The wound repair and proliferation of bronchial epithelial cells (BECs) were accelerated in a concentration-dependent manner by BRS-3 activation with P3513, which could be inhibited by PKA inhibitor H89. The study demostrated that activation of BRS-3 may play an important role in wound repair of AHR.
Tan
Yu-Rong
YR
Xiangya School of Medicine, Central South University, Changsha 410078, Hunan, China.
Qi
Ming-Ming
MM
Qin
Xiao-Qun
XQ
Xiang
Yang
Y
Li
Xiang
X
Wang
Yue
Y
Qu
Fei
F
Liu
Hui-Jun
HJ
Zhang
Jian-Song
JS
D000818:Animals; D001980:Bronchi / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology*; D007546:Isoquinolines / Q000494:pharmacology; D008648:Mesoderm / Q000378:metabolism; D018715:Microscopy, Video; D011817:Rabbits; D018004:Receptors, Bombesin / Q000737:chemistry* / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology; D014945:Wound Healing
D004791:Enzyme Inhibitors; D007546:Isoquinolines; D018004:Receptors, Bombesin; D013449:Sulfonamides; C107120:bombesin receptor subtype 3; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide