Cannabinoids and dopamine receptors' action on calcium current in rat neurons.
OBJECTIVE
To study the effects of cannabinoid, glutamate, and dopamine agonists and antagonists on the calcium current rat sympathetic neurons.


METHODS
Calcium current was recorded using the whole-cell variant of the patch-clamp technique. After expression in neuronal membranes of the cannabinoid CB1, glutamate mGluR2, or dopamine D1 receptor (by microinjection of the levant receptor's cDNA into the neuron's nucleus) agonists' and antagonists' effects were observed.


RESULTS
Applications of agonists of the expressed receptor (0.1-10 microM) decreased the calcium current. The calcium current was increased after application of cannabinoid antagonists (AM251 and AM630); these compounds thus act as inverse agonists in this preparation. Glutamate and dopamine antagonists had no effects on the calcium current by themselves. Combined application of cannabinoids and dopamine, but not glutamate, agonists produced a decrement in the calcium current that was bigger than either of the effects seen when one agonist was applied alone.


CONCLUSIONS
These results suggest that cannabinoid with dopamine receptors have an interactive inhibitory effect on the calcium current in this preparation, indicating that within the nervous system, receptor interactions may be important in the regulation of ion-channel functions.
Vásquez
C
C
Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colonia Villa de San Sebastián, Colima, CP, Mexico.
Navarro-Polanco
R
R
Hernández
G
G
Ruiz
J
J
Guerra
D G
DG
Baltazar
L M
LM
Huerta
M
M
Trujillo
X
X
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D043884:Receptor, Cannabinoid, CB1 / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017447:Receptors, Dopamine D1 / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017783:Superior Cervical Ganglion / Q000166:cytology / Q000378:metabolism
D043884:Receptor, Cannabinoid, CB1; D017447:Receptors, Dopamine D1; D018094:Receptors, Metabotropic Glutamate; C108822:metabotropic glutamate receptor 2; D002118:Calcium