title: Effect of bioaugmentation and supplementary carbon sources on degradation of polycyclic aromatic hydrocarbons by a soil-derived culture.
abstract: The degradation of polycyclic aromatic hydrocarbons (PAHs) by an undefined culture obtained from a PAH-polluted soil and the same culture bioaugmented with three PAH-degrading strains was studied in carbon-limited chemostat cultures. The PAHs were degraded efficiently by the soil culture and bioaugmentation did not significantly improve the PAH degrading performance. The presence of PAHs did, however, influence the bacterial composition of the bioaugmented and non-bioaugmented soil cultures, resulting in the increase in cell concentration of sphingomonad strains. the initial enhancement of the degradation of the PAHs by biostimulation gradually disappeared and only the presence of salicylate in the additional carbon sources had a lasting slightly stimulating effect on the degradation of phenanthrene. The results suggest that bioaugmentation and biostimulation have limited potential to enhance PAH bioremediation by culture already proficient in the degradation of such contaminants.
authors.affiliation: Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, the Netherlands.
authors.forename: René
authors.initials: R
authors.lastname: van Herwijnen
authors.forename: Boris
authors.initials: B
authors.lastname: Joffe
authors.forename: Annemie
authors.initials: A
authors.lastname: Ryngaert
authors.forename: Martina
authors.initials: M
authors.lastname: Hausner
authors.forename: Dirk
authors.initials: D
authors.lastname: Springael
authors.forename: Harrie A J
authors.initials: HA
authors.lastname: Govers
authors.forename: Stefan
authors.initials: S
authors.lastname: Wuertz
authors.forename: John R
authors.initials: JR
authors.lastname: Parsons
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D004586:Electrophoresis; D004784:Environmental Monitoring; D017404:In Situ Hybridization, Fluorescence; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification; D010616:Phenanthrenes / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012459:Salicylates; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D020578:Sphingomonas / Q000235:genetics / Q000302:isolation & purification; D018505:Waste Management / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D012459:Salicylates; D012989:Soil Pollutants; C031181:phenanthrene