Myceligenerans crystallogenes sp. nov., isolated from Roman catacombs.
Three xylan-degrading actinobacterial strains were isolated from different sampling sites in the Roman catacombs of Domitilla and San Callisto. The organisms showed morphological and chemotaxonomic properties such as peptidoglycan type A4alpha, L-lys-L-thr-D-Glu; whole-cell sugars (glucose, mannose and galactose); octa-, hexa- and tetrahydrogenated menaquinones with nine isoprene units; phosphatidylglycerol and diphosphatidylglycerol as the major phospholipids; anteiso-C(15 : 0), iso-C(15 : 0) and iso-C(16 : 0) as the predominant fatty acids; and a DNA G+C content of 72 mol%. These features are consistent with affiliation of these isolates to the genus Myceligenerans. The three isolates shared a 16S rRNA gene similarity of 99.9 % and were most closely related to Myceligenerans xiligouense DSM 15700T (97.9 % sequence similarity). The low level of DNA-DNA relatedness (about 14 %) and the differences in phenotypic characteristics between the novel strains and M. xiligouense DSM 15700T justify the proposal of a novel species of the genus Myceligenerans, Myceligenerans crystallogenes sp. nov., with CD12E2-27T (= HKI 0369T = DSM 17134T = NCIMB 14061T = VTT E-032285T) as the type strain.
Groth
Ingrid
I
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V., Hans-Knöll-Institut, Beutenbergstrasse 11a, 07745 Jena, Germany. Ingrid.Groth@hki-jena.de
Schumann
Peter
P
Schütze
Barbara
B
Gonzalez
Juan M
JM
Laiz
Leonila
L
Suihko
Maija-Liisa
ML
Stackebrandt
Erko
E
D039903:Actinobacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D001106:Archaeology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001483:Base Sequence; D001673:Biodegradation, Environmental; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012384:Rome; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013045:Species Specificity; D014990:Xylans / Q000378:metabolism
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014990:Xylans