title: Variations of dopamine, serotonin, and amino acid concentrations in Noda epileptic rat (NER) retina.
abstract: Noda epileptic rats (NER) exhibit frequent spontaneous tonic-clonic convulsions which represent a valuable model of human epilepsy. If implication of brain neurotransmitters was largely reported, little is known about retina. However, it has been reported that human epilepsy syndrome varies not only with the location of seizure foci but also according to rhythmic patterns, for which retina has a major role in the transmission of external light-dark cycle information. The purpose of this work was to evaluate dopamine (DA), DA metabolites, serotonin (5-HT), and amino acid [glutamate, aspartate, glycine, gamma aminobutyric acid (GABA), and taurine] level variations in retina from NER, at two different nycthemeral periods (11 a.m. and 11 p.m.) and at different ages (2, 6, and 12 months). In NER, retinal dopaminergic function was decreased as soon as 2 months, whereas GABA levels were increased, even if no differences among the different ages could be distinguished. These variations were associated to a slight increase in 5-HT. Other amino acids tested were not affected by epilepsy, whereas taurine decreased with aging in NER as well as in control rats. Retinal 5-HT occurs principally as a precursor of melatonin (MEL). A triangular interaction may be hypothesized: MEL could decrease DA synthesis or release by enhancing GABA activity. Taken together, these results suggest that the retinal physiology is affected by the epileptic status and that information transmitted from retina to the brain should be affected by epilepsy in NER.
authors.affiliation: Laboratoire de Pharmacologie, Faculté de Pharmacie, 92296 Châtenay-Malabry, France. evelyne.chanut@wanadoo.fr
authors.forename: Evelyne
authors.initials: E
authors.lastname: Chanut
authors.forename: Benoît
authors.initials: B
authors.lastname: Labarthe
authors.forename: Brigitte
authors.initials: B
authors.lastname: Lacroix
authors.forename: Atsuhi
authors.initials: A
authors.lastname: Noda
authors.forename: Sylvie
authors.initials: S
authors.lastname: Gasdeblay
authors.forename: Jean-Robert
authors.initials: JR
authors.lastname: Bondier
authors.forename: Claudine
authors.initials: C
authors.lastname: Versaux-Botteri
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000375:Aging / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D015306:Biogenic Monoamines / Q000378:metabolism; D002940:Circadian Rhythm; D004298:Dopamine / Q000031:analogs & derivatives / Q000378:metabolism*; D004827:Epilepsy / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D006719:Homovanillic Acid / Q000378:metabolism; D008297:Male; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar; D012160:Retina / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D015306:Biogenic Monoamines; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; C001746:3-methoxytyramine; D004298:Dopamine; D006719:Homovanillic Acid