Adiabatic JHSQC for 13C isotopomer analysis.
A previous implementation of JHSQC enhanced the detection limits for 13C isotopomer analysis, but owing to experimental instabilities required extensive calibration prior to data collection. The addition of adiabatic pulses as well as a double spin-echo during the heteronuclear transfer periods has dramatically increased the precision of the experiment and done away with the need for calibration prior to running each sample. This new experiment allows the sensitivity gains from inverse detection to be realized in a time-efficient manner for the experimentalist.
Merritt
Matthew E
ME
University of Texas Southwestern Medical Center, Advanced Imaging Research Center, 2201 Inwood Road, NE 4.204, Dallas, TX 775390-8568, USA. matthew.merritt@utsouthwestern.edu
Burgess
Shawn C
SC
Spitzer
Timothy D
TD
D002247:Carbon Isotopes / Q000032:analysis* / Q000737:chemistry; D002952:Citric Acid Cycle / Q000502:physiology; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D018698:Glutamic Acid / Q000032:analysis / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002247:Carbon Isotopes; D018698:Glutamic Acid; D005947:Glucose