A divergent external loop confers antagonistic activity on floral regulators FT and TFL1.
The Arabidopsis genes FT and TERMINAL FLOWER1 (TFL1) encode related proteins with similarity to human Raf kinase inhibitor protein. FT, and likely also TFL1, is recruited to the promoters of floral genes through interaction with FD, a bZIP transcription factor. FT, however, induces flowering, while TFL1 represses flowering. Residues responsible for the opposite activities of FT and TFL1 were mapped by examining plants that overexpress chimeric proteins. A region important in vivo localizes to a 14-amino-acid segment that evolves very rapidly in TFL1 orthologs, but is almost invariant in FT orthologs. Crystal structures show that this segment forms an external loop of variable conformation. The only residue unambiguously distinguishing the FT and TFL1 loops makes a hydrogen bond with a residue near the entrance of a potential ligand-binding pocket in TFL1, but not in FT. This pocket is contacted by a C-terminal peptide, which also contributes to the opposite FT and TFL1 activities. In combination, these results identify a molecular surface likely to be recognized by FT- and/or TFL1-specific interactors.
Ahn
Ji Hoon
JH
Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.
Miller
David
D
Winter
Victoria J
VJ
Banfield
Mark J
MJ
Lee
Jeong Hwan
JH
Yoo
So Yeon
SY
Henz
Stefan R
SR
Brady
Robert Leo
RL
Weigel
Detlef
D
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005091:Exons; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D010802:Phylogeny; D030821:Plants, Genetically Modified; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; C418905:FT protein, Arabidopsis; D010455:Peptides; D011993:Recombinant Fusion Proteins; C102864:TFL1 protein, Arabidopsis