title: Cecal spirochetosis caused by Brachyspira pilosicoli in commercial turkeys.
abstract: Spirochetes that were identified as Brachyspira pilosicoli were present in the ceca of 7.5- to 18-wk-old turkeys with cecal spirochetosis and typhlitis. The identity of B. pilosicoli was confirmed on the basis of ultrastructural morphology of the cecal epithelium adherent microbes, immunohistochemical staining with a Brachyspira genus-specific monoclonal antibody, and amplification of a B. pilosicoli species-specific 16S ribosomal RNA (rrs gene) sequence by using the polymerase chain reaction and DNA obtained by laser-capture microdissection of the epithelium-adherent microbial fringe. To the author's knowledge, this is the first report of B. pilosicoli in the ceca of turkeys.
authors.affiliation: California Animal Health and Food Safety Laboratory System, Fresno Branch University of California, Davis, 2789 S. Orange Avenue, Fresno, CA 93725, USA.
authors.forename: H L
authors.initials: HL
authors.lastname: Shivaprasad
authors.forename: G E
authors.initials: GE
authors.lastname: Duhamel
mesh_terms: D000818:Animals; D001483:Base Sequence; D002432:Cecum / Q000382:microbiology / Q000473:pathology; D005798:Genes, Bacterial; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011201:Poultry Diseases / Q000382:microbiology* / Q000473:pathology; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013144:Spirochaetales / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D013145:Spirochaetales Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D014422:Turkeys / Q000382:microbiology*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S