Mouse model of respiratory Chlamydia pneumoniae infection for a genomic screen of subunit vaccine candidates.
An inbred A/J mouse respiratory challenge model was validated for vaccine testing against Chlamydia (C.) pneumoniae and used to screen the C. pneumoniae genome for vaccine candidates by expression library immunization (ELI). Biolistic delivery of genetic vaccine constructs elicited Th2-like immunity that was associated with inefficient elimination of C. pneumoniae. Delivery by injection elicited protective Th1-like responses. Since biolistic delivery of pools of ORFs was used in first round screening, the screen presumably selected against potent immunogens. Nevertheless, it was sufficiently accurate to identify three weakly protective antigens among all putative C. pneumoniae ORFs. The results suggest ELI discovery of highly protective C. pneumoniae vaccine candidates requires tight control of the Th1 immunity elicited by the genetically delivered library of test antigens.
Li
Dan
D
Department of Pathobiology, College of Veterinary Medicine, Auburn University, 270 Greene Hall, Auburn, AL 36849-5519, USA.
Borovkov
Alexander
A
Vaglenov
Alexander
A
Wang
Chengming
C
Kim
Teayoun
T
Gao
Dongya
D
Sykes
Kathryn F
KF
Kaltenboeck
Bernhard
B
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D019470:Biolistics; D023521:Chlamydophila Infections / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D004195:Disease Models, Animal; D005260:Female; D050990:GATA3 Transcription Factor / Q000235:genetics; D015870:Gene Expression; D000072597:Hepatitis A Virus Cellular Receptor 2; D008168:Lung / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018410:Pneumonia, Bacterial / Q000276:immunology* / Q000517:prevention & control; D012333:RNA, Messenger / Q000032:analysis; D011991:Receptors, Virus / Q000235:genetics; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; C442241:Havcr2 protein, mouse; D000072597:Hepatitis A Virus Cellular Receptor 2; D012333:RNA, Messenger; D011991:Receptors, Virus