An auxin-driven polarized transport model for phyllotaxis.
Recent studies show that plant organ positioning may be mediated by localized concentrations of the plant hormone auxin. Auxin patterning in the shoot apical meristem is in turn brought about by the subcellular polar distribution of the putative auxin efflux mediator, PIN1. However, the question of what signals determine PIN1 polarization and how this gives rise to regular patterns of auxin concentration remains unknown. Here we address these questions by using mathematical modeling combined with confocal imaging. We propose a model that is based on the assumption that auxin influences the polarization of its own efflux within the meristem epidermis. We show that such a model is sufficient to create regular spatial patterns of auxin concentration on systems with static and dynamic cellular connectivities, the latter governed by a mechanical model. We also optimize parameter values for the PIN1 dynamics by using a detailed auxin transport model, for which parameter values are taken from experimental estimates, together with a template consisting of cell and wall compartments as well as PIN1 concentrations quantitatively extracted from confocal data. The model shows how polarized transport can drive the formation of regular patterns.
Jönsson
Henrik
H
Computational Biology and Biological Physics Group, Department of Theoretical Physics, Lund University, S-221 00 Lund, Sweden.
Heisler
Marcus G
MG
Shapiro
Bruce E
BE
Meyerowitz
Elliot M
EM
Mjolsness
Eric
E
D017360:Arabidopsis / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry; D001692:Biological Transport; D049109:Cell Proliferation; D002633:Chemotaxis; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007210:Indoleacetic Acids / Q000737:chemistry* / Q000378:metabolism; D026901:Membrane Transport Proteins; D018519:Meristem; D018613:Microscopy, Confocal; D008954:Models, Biological; D015233:Models, Statistical; D018521:Plant Physiological Phenomena; D013997:Time Factors
D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D049452:Green Fluorescent Proteins