A new RP-HPLC method for analysis of meloxicam in tablets.
A simple and rapid high-performance liquid chromatographic (HPLC) method for the determination and quantification of meloxicam has been developed. The chromatographic system consisted of a Shimadzu LC-10 AT VP pump, SPD-10 AV VP UV/ visible detector, and a CBM-102 Bus Module integrator. Separation was achieved on the micro Bondapak 125 A C18 10 microm column at room temperature. The sample was introduced through an injector valve with a 10 microl sample loop. Methanol:water (70:30 v/v) was used as mobile phase, with flow rate 2 ml/minutes. pH was adjusted to 2.6 with phosphoric acid. U.V detection was performed at 230 nm. The results obtained showed a good agreement with the declared content. Recovery values of meloxicam in tablets (in Melfax 15 mg tablets) were from 99.27 % to 100.06 %. Piroxicam was used as an internal standard. The proposed method is rapid, accurate and selective; it may be used for the quantitative analysis of meloxicam from raw materials, in bulk drugs and other dosage formulations.
Arayne
M Saeed
MS
Department of Chemistry, University of Karachi, Karachi-75270, Pakistan.
Sultana
Najma
N
Siddiqui
Farhan Ahmed
FA
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000077239:Meloxicam; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets; D013843:Thiazines / Q000032:analysis* / Q000737:chemistry; D013844:Thiazoles / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D013607:Tablets; D013843:Thiazines; D013844:Thiazoles; D000077239:Meloxicam