Developmental and amyloid plaque-related changes in cerebral cortical capillaries in transgenic Tg2576 Alzheimer mice.
There is experimental evidence that cerebral perfusion is decreased during aging and in Alzheimer's disease. To characterize the temporal relationship between amyloid deposition, plaque size and cerebrovascular abnormalities, a semiquantitative immunohistochemical study was performed in transgenic Tg2576 mice that express the Swedish double mutation of human amyloid precursor protein (APP) and progressively develop Alzheimer-like beta-amyloid deposits. Cortical cryocut sections, obtained from mice at ages ranging between 4 and 18 months, were immunostained to label glucose transporter type 1 (GLUT1), a marker of vascular endothelial cells, and thioflavine-S to visualize plaques. Regardless of age and transgene, a laminar distribution of capillaries was observed being highest in cortical layers IV and V. The density of microvessels estimated in cortical regions with high plaque load was found to be significantly lower as compared to areas with low plaque load. Around large thioflavine-S-positive senile plaques the capillary density was low, while diffuse plaques demonstrated a close association of capillaries with no signs of any damage. The data suggest that amyloid plaque deposition differentially affects the cerebrovascular system in an age- and plaque type-related manner, and provide further evidence that beta-amyloid, in addition to its well-described neurotoxic effects, may also contribute to neuronal dysfunction through its actions on the cerebrovasculature.
Kouznetsova
Elena
E
Paul Flechsig Institute for Brain Research, University of Leipzig, Department of Neurochemistry, Jahnallee 59, D-04109 Leipzig, Germany.
Klingner
Margrit
M
Sorger
Dietlind
D
Sabri
Osama
O
Grossmann
Udo
U
Steinbach
Jörg
J
Scheunemann
Matthias
M
Schliebs
Reinhard
R
D000367:Age Factors; D000544:Alzheimer Disease / Q000235:genetics / Q000473:pathology*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002196:Capillaries / Q000254:growth & development / Q000378:metabolism / Q000473:pathology; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000379:methods; D058225:Plaque, Amyloid / Q000378:metabolism*; D018709:Statistics, Nonparametric
D016564:Amyloid beta-Protein Precursor; D051272:Glucose Transporter Type 1