Neural network-based analysis of thiol proteomics data in identifying potential selenium targets.
Generation of a monomethylated selenium metabolite is critical for the anticancer activity of selenium. Because of its strong nucleophilicity, the metabolite can react directly with protein thiols to cause redox modification. Here, we report a neural network-based analysis to identify potential selenium targets. A reactive thiol specific reagent, BIAM, was used to monitor thiol proteome changes on 2D gel. We constructed a dynamic model and evaluated the relative importance of proteins mediating the cellular responses to selenium. Information from this study will provide new clues to unravel mechanisms of anticancer action of selenium. High impact selenium targets could also serve as biomarkers to gauge the efficacy of selenium chemoprevention.
Lee
Jong-Sik
JS
School of Computer Science & Engineering, Inha University, Korea.
Ma
Yong-Beom
YB
Choi
Kyoung-Soo
KS
Park
Soo-Yeon
SY
Baek
Sun-Hee
SH
Park
Young-Mee
YM
Zu
Ke
K
Zhang
Haitao
H
Ip
Clement
C
Kim
Yeul Hong
YH
Park
Eun-Mi
EM
D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D005029:Ethylenediamines / Q000737:chemistry; D006801:Humans; D008297:Male; D016571:Neural Networks, Computer; D016566:Organoselenium Compounds / Q000378:metabolism / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012643:Selenium / Q000378:metabolism / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
D000970:Antineoplastic Agents; D005029:Ethylenediamines; C093128:N-biotinoyl-N'-iodoacetylethylenediamine; D016566:Organoselenium Compounds; D011506:Proteins; D020543:Proteome; D013438:Sulfhydryl Compounds; D001710:Biotin; C008493:methylselenic acid; D012643:Selenium