Production of saccharogenic and dextrinogenic amylases by Rhizomucor pusillus A 13.36.
A newly-isolated thermophilic strain of the zygomycete fungus Rhizomucor pusillus 13.36 produced highly active dextrinogenic and saccharogenic enzymes. Cassava pulp was a good alternative substrate for amylase production. Dextrinogenic and saccharogenic amylases exhibited optimum activities at a pH of 4.0-4.5 and 5.0 respectively and at a temperature of 75 degrees C. The enzymes were highly thermostable, with no detectable loss of saccharogenic or dextrinogenic activity after 1 h and 6 h at 60 degrees C, respectively. The saccharogenic activity was inhibited by Ca(2+) while the dextrinogenic was indifferent to this ion. Both activities were inhibited by Fe(2+) and Cu(2+) Hydrolysis of soluble starch by the crude enzyme yielded 66% glucose, 19.5% maltose, 7.7% maltotriose and 6.6% oligosaccharides.
Silva
Tony M
TM
Laboratorio de Bioquimica e Microbiologia, IBILCE-Instituto de Instituto de Biociencias, Letras e Ciencias Exatas, UNESP- Universidade Estadual Paulista., Sao Jose do Rio Preto- SP, Brazil.
Attili-Angeli
Derlene
D
Carvalho
Ana Flavia Azeved
AF
Da Silva
Roberto
R
Boscolo
Mauricio
M
Gomes
Eleni
E
D000681:Amylases / Q000096:biosynthesis*; D002118:Calcium / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology; D008320:Maltose / Q000378:metabolism; D002365:Manihot / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D020103:Rhizomucor / Q000201:enzymology*; D013213:Starch / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014312:Trisaccharides / Q000378:metabolism
D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D014312:Trisaccharides; D013395:Sucrose; C008317:maltotriose; D008320:Maltose; D003300:Copper; D013213:Starch; D007501:Iron; D000681:Amylases; D005947:Glucose; D002118:Calcium