title: Effects of Crotalus durissus collilineatus venom in the isolated rat kidney.
abstract: Ophidian accidents caused by the subspecies Crotalus durissus are responsible for high morbity and mortality rates. Acute renal failure is a common complication observed in these accidents. The aim of the present study was to investigate the renal effects promoted by the venom of C. d. collilineatus and its fractions, crotoxin and phospholipase A2. C. d. collilineatus (Cdc; 30 microg mL(-1)), crotoxin (CTX; 10 microg mL(-1)) and phospholipase A2 (PLA2; 10 microg mL(-1)) were tested in isolated rat kidney. The first 30 min of each experiment were used as an internal control and Cdc or its fractions, CTX and PLA2 were added to the system after this period. All experiments lasted 120 min. The venom of Cdc decreased perfusion pressure (PP; control120 = 110.3 +/- 3.69 mmHg; Cdc120 = 96.7+/-8.1 mmHg), renal vascular resistance (RVR; control120 = 6.42+/-0.78 mmHg mL g(-1) min(-1); Cdc120 = 4.8+/-0.56 mmHg/mL g(-1) min(-1)), urinary flow (UF; control120 = 0.19+/-0.03 mL g(-1) min(-1); Cdc120 = 0.12 +/- 0.01 mL g(-1) min(-1)), and glomerular filtration rate (GFR; control120 = 0.79 +/- 0.07 mL g(-1) min(-1); Cdc120 = 0.53 +/- 0.09 mL g(-1) min(-1)), but had no effect on the percent of sodium tubular transport (%TNa+), percent of chloride tubular transport (%TK+) and percent of potassium tubular transport (%TCl-). CTX and PLA2 reduced the GFR, while UF, PP and RVR remained stable during the full 120 min of perfusion. Crotoxin administration also diminished the %TK+ (control120 = 69.94 +/- 6.49; CTX120 = 33.28 +/- 4.78) and %TCl- (control120 = 79.53 +/- 2.67; CTX120 = 64.62 +/- 6.93). PLA2 reduced the %TK+, but exerted no effect on the %TNa+ or on that of TCl-. In conclusion, the C. d. collilineatus venom altered the renal functional parameters evaluated. We suggest that crotoxin and phospholipase A2 were involved in this process, since the renal effects observed would be due to the synergistic action of the components of the venom.
authors.affiliation: Department of Physiology and Pharmacology, Institute of Biomedicine and Clinical Research Unit, Federal University of Ceara, Fortaleza, Ceara, Brazil. daniela_amora@bol.com.br
authors.forename: Daniela N
authors.initials: DN
authors.lastname: Amora
authors.forename: Ticiana M
authors.initials: TM
authors.lastname: Sousa
authors.forename: Alice M C
authors.initials: AM
authors.lastname: Martins
authors.forename: Paulo S F
authors.initials: PS
authors.lastname: Barbosa
authors.forename: Marta R
authors.initials: MR
authors.lastname: Magalhães
authors.forename: Marcus H
authors.initials: MH
authors.lastname: Toyama
authors.forename: Manassés C
authors.initials: MC
authors.lastname: Fonteles
authors.forename: Dalgimar B
authors.initials: DB
authors.lastname: de Menezes
authors.forename: Helena S A
authors.initials: HS
authors.lastname: Monteiro
mesh_terms: D000818:Animals; D003435:Crotalid Venoms / Q000008:administration & dosage / Q000494:pharmacology*; D017839:Crotalus; D003439:Crotoxin / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007668:Kidney / Q000187:drug effects* / Q000502:physiology; D008297:Male; D010741:Phospholipases A / Q000008:administration & dosage / Q000494:pharmacology; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar; D014655:Vascular Resistance / Q000187:drug effects
chemical_list: D003435:Crotalid Venoms; D003439:Crotoxin; D010741:Phospholipases A; D054467:Phospholipases A2