title: Potential for alternative intron-exon pairings in group II intron RmInt1 from Sinorhizobium meliloti and its relatives.
abstract: Ribozyme constructs derived from group II intron RmInt1 of Sinorhizobium meliloti self-splice in vitro when incubated under permissive conditions, but exon ligation is unusually inefficient when the 5' exon is truncated close to the IBS2 intron-binding site. One plausible explanation for this observation is the presence of an alternative intron-exon pairing between an intron segment that overlaps with the EBS2 exon-binding site and a 5' exon site located just distal of IBS2 relative to the splice junction. Strikingly, the existence of this pairing is supported by comparative sequence analysis of introns related to RmInt1.
authors.forename: María
authors.initials: M
authors.lastname: Costa
authors.forename: François
authors.initials: F
authors.lastname: Michel
authors.forename: Nicolás
authors.initials: N
authors.lastname: Toro
mesh_terms: D017398:Alternative Splicing; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D005091:Exons; D007438:Introns; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000201:enzymology / Q000235:genetics*
chemical_list: D012329:RNA, Bacterial; D016337:RNA, Catalytic