Mapping quantitative trait loci in noninbred mosquito crosses.
The identification of genes that affect quantitative traits has been of great interest to geneticists for many decades, and many statistical methods have been developed to map quantitative trait loci (QTL). Most QTL mapping studies in experimental organisms use purely inbred lines, where the two homologous chromosomes in each individual are identical. As a result, many existing QTL mapping methods developed for experimental organisms are applicable only to genetic crosses between inbred lines. However, it may be difficult to obtain inbred lines for certain organisms, e.g., mosquitoes. Although statistical methods for QTL mapping in outbred populations, e.g., humans, can be applied for such crosses, these methods may not fully take advantage of the uniqueness of these crosses. For example, we can generally assume that the two grandparental lines are homozygous at the QTL of interest, but such information is not be utilized through methods developed for outbred populations. In addition, mating types and phases can be relatively easy to establish through the analysis of adjacent markers due to the large number of offspring that can be collected, substantially simplifying the computational need. In this article, motivated by a mosquito intercross experiment involving two selected lines that are not genetically homozygous across the genome, we develop statistical methods for QTL mapping for genetic crosses involving noninbred lines. In our procedure, we first infer parental mating types and use likelihood-based methods to infer phases in each parent on the basis of genotypes of offspring and one parent. A hidden Markov model is then employed to estimate the number of high-risk alleles at marker positions and putative QTL positions between markers in each offspring, and QTL mapping is finally conducted through the inferred QTL configuration across all offspring in all crosses. The performance of the proposed methods is assessed through simulation studies, and the usefulness of this method is demonstrated through its application to a mosquito data set.
Wang
Shuang
S
Department of Biostatistics, Mailman School of Public Health, Columbia University, New York, New York 10032, USA.
Huang
Song
S
Zheng
Liangbiao
L
Zhao
Hongyu
H
D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D009033:Culicidae / Q000235:genetics*; D005260:Female; D005821:Genetic Techniques; D006720:Homozygote; D008297:Male; D008390:Markov Chains; D008957:Models, Genetic; D015233:Models, Statistical; D011336:Probability; D040641:Quantitative Trait Loci / Q000235:genetics*