A critical role for the type V myosin, Myo52, in septum deposition and cell fission during cytokinesis in Schizosaccharomyces pombe.
Cytokinesis in fission yeast involves the coordination of septum deposition with the contraction of a cytokinetic actomyosin ring. We have examined the role of the type V myosin Myo52 in the coupling of these two events by the construction of a series of deletion mutants of the Myo52 tail and a further mutant within the ATP binding domain of the head. Each mutant protein was ectopically expressed in fission yeast cells. Each truncation was assayed for the ability to localize to the cell poles and septum (the normal cellular locations of Myo52) and to rescue the morphology defects and temperature sensitivity of a myo52Delta strain. A region within the Myo52 tail (amino acids 1320-1503), with a high degree of similarity to the vesicle-binding domain of the budding yeast type V myosin Myo2p, was essential for Myo52's role in the maintenance of growth polarity and cell division. A separate region (amino acids 1180-1320) was required for Myo52 foci to move throughout the cytoplasm; however, constructs lacking this region, but which retained the ability to dimerize still associated with actin at sites of cell growth. Not all of the Myo52 truncations which localized rescued the morphological defects of myo52Delta, demonstrating that loss of function was not simply brought about by an inability of mutant proteins to target the correct cellular location. By contrast, Myo52 motor activity was required for both localization and cellular function. myo52Delta cells were unable to efficiently localize the beta-1,3-glucan synthase, Bgs1, either at the cell poles or at the division septum, regions of cell wall deposition. Bgs1 and Myo52 localized to vesicle-like dots at the poles in interphase and these moved together to the septum at division. These data have led to the formulation of a model in which Myo52 is responsible for the delivery of Bgs1 and associated molecules to polar cell growth regions during interphase. On the commencement of septum formation, Myo52 transports Bgs1 to the cell equator, thus ensuring the accurate deposition of beta-1,3-glucan at the leading edge of the primary septum.
Mulvihill
Daniel P
DP
Department of Biology, University College London, London WC1E 6BT, United Kingdom. d.p.mulvihill@kent.ac.uk
Edwards
Suzanne R
SR
Hyams
Jeremy S
JS
D000595:Amino Acid Sequence; D002473:Cell Wall / Q000378:metabolism*; D048749:Cytokinesis / Q000502:physiology*; D005964:Glucosyltransferases; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D024701:Myosin Type V / Q000235:genetics / Q000502:physiology*; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000235:genetics; D011509:Proteoglycans; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017384:Sequence Deletion; D047071:beta-Glucans / Q000378:metabolism
D008565:Membrane Proteins; D011509:Proteoglycans; D029702:Schizosaccharomyces pombe Proteins; D047071:beta-Glucans; C010770:polysaccharide-K; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase; D024701:Myosin Type V