title: Nitrate reductase gene involvement in hexachlorobiphenyl dechlorination by Phanerochaete chrysosporium.
abstract: Polychlorobiphenyl (PCB) degradation usually occurs through reductive dechlorination under anaerobic conditions and phenolic ring cleavage under aerobic conditions. In this paper, we provide evidence of nitrate reductase (NaR) mediated dechlorination of hexachlorobiphenyl (PCB-153) in Phanerochaete chrysosporium under non-ligninolytic condition and the gene involved. The NaR enzyme and its cofactor, molybdenum (Mo), were found to mediate reductive dechlorination of PCBs even in aerobic condition. Tungsten (W), a competitive inhibitor of this enzyme, was found to suppress this dechlorination. Chlorine release assay provided further evidence of this nitrate reductase mediated dechlorination. Commercially available pure NaR enzyme from Aspergillus was used to confirm these results. Through homology search using TBLASTN program, NaR gene was identified, primers were designed and the RT-PCR product was sequenced. The NaR gene was then annotated in the P. chrysosporium genome (GenBank accession no. AY700576). This is the first report regarding the presence of nitrate reductase gene in this fungus with the explanation why this fungus can dechlorinate PCBs even in aerobic condition. These fungal inoculums are used commercially as pellets in sawdust for enhanced bioremediation of PCBs at the risk of depleting soil nitrates. Hence, the addition of nitrates to the pellets will reduce this risk as well as enhance its activity.
authors.affiliation: Department of Biology, Howard University, Washington, DC 20059, USA.
authors.forename: Supriyo
authors.initials: S
authors.lastname: De
authors.forename: Michael
authors.initials: M
authors.lastname: Perkins
authors.forename: Sisir K
authors.initials: SK
authors.lastname: Dutta
mesh_terms: D001483:Base Sequence; D002713:Chlorine / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D050901:Nitrate Reductase / Q000235:genetics* / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics; D011078:Polychlorinated Biphenyls / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D002713:Chlorine; D011078:Polychlorinated Biphenyls; D050901:Nitrate Reductase