title: Diversity of the dsrAB (dissimilatory sulfite reductase) gene sequences retrieved from two contrasting mudflats of the Seine estuary, France.
abstract: The diversity of sulfate-reducing microorganisms was investigated in two contrasting mudflats of the Seine estuary, by PCR amplification, cloning and sequencing of the genes coding for parts of the alpha and beta subunits of dissimilatory sulfite reductase (dsrAB). One site is located in the mixing-zone and shows marine characteristics, with high salinity and sulfate concentration, whereas the other site shows freshwater characteristics, with low salinity and sulfate concentration. Diversity and abundance of dsrAB genes differed between the two sites. In the mixing-zone sediments, most of the dsrAB sequences were affiliated to those of marine Gram-negative bacteria belonging to the order of Desulfobacterales, whereas in the freshwater sediments, a majority of dsrAB sequences was related to those of the Gram-positive bacteria belonging to the genus Desulfotomaculum. It is speculated that this is related to the salinity and the sulfate concentration in the two mudflats.
authors.affiliation: Laboratoire de Microbiologie Du Froid, Groupe Biodiversité et Environnement, UPRES-2123, Université de Rouen, Mont Saint Aignan, France. jleloup@mpi-bremen.de
authors.forename: Julie
authors.initials: J
authors.lastname: Leloup
authors.forename: Laurent
authors.initials: L
authors.lastname: Quillet
authors.forename: Thierry
authors.initials: T
authors.lastname: Berthe
authors.forename: Fabienne
authors.initials: F
authors.lastname: Petit
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D045859:Desulfotomaculum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005602:France; D014644:Genetic Variation; D019015:Geologic Sediments / Q000382:microbiology*; D050885:Hydrogensulfite Reductase / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D050885:Hydrogensulfite Reductase