Molecular detection of Paracoccidioides brasiliensis in soil.
The objective of the present study was to evaluate different techniques for the detection of Paracoccidioides brasiliensis in soil, e.g., culture, animal inoculation and specific DNA amplification by Nested PCR. We designed species-specific inner primers derived from rDNA regions (ITS, 5.8S gene) and found their sensitivity to be higher than culture and animal inoculation. In addition, the sensitivity of these primers was higher than p27-gene primers developed for detection of P. brasiliensis in soil in a previous study. DNA from P. brasiliensis was detected in soil artificially seeded with the fungus (positive soil control) and from environmental samples collected in an armadillo burrow.
Theodoro
Raquel Cordeiro
RC
Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, Botucatu, SP Brasil.
Candeias
João Manuel Grisi
JM
Araújo
João Pessoa
JP
Bosco
Sandra De Moraes Gimenes
Sde M
Macoris
Severino Assis Da Graça
SA
Padula
Luis Otavio Junior
LO
Franco
Marcello
M
Bagagli
Eduardo
E
D001938:Brazil; D004271:DNA, Fungal / Q000032:analysis*; D021901:DNA, Intergenic / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D009172:Mycology / Q000379:methods; D010228:Paracoccidioides / Q000254:growth & development / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D012680:Sensitivity and Specificity; D012988:Soil Microbiology
D004271:DNA, Fungal; D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S