title: Detection of cdtA, cdtB, and cdtC genes in Campylobacter jejuni by multiplex PCR.
abstract: A multiplex PCR was developed for simultaneous detection of the cytolethal distending toxin (cdt) genes of Campylobacter jejuni. Three primer pairs targeting each one of the cdtA, cdtB and cdtC genes were designed and combined in the same PCR reaction. The assay was evaluated with 100 C. jejuni strains recovered from humans and animals and it was found to be rapid and specific. Two isolates presented several deletions affecting both cdtA and cdtB genes. High prevalence (98%) of the three cdt genes was found among isolates of different geographic origins.
authors.affiliation: Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV-EHU), Paseo de la Universidad, 7 1, E-01006 Vitoria-Gasteiz, Spain.
authors.forename: Irati
authors.initials: I
authors.lastname: Martínez
authors.forename: Estibaliz
authors.initials: E
authors.lastname: Mateo
authors.forename: Estibaliz
authors.initials: E
authors.lastname: Churruca
authors.forename: Cecilia
authors.initials: C
authors.lastname: Girbau
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Alonso
authors.forename: Aurora
authors.initials: A
authors.lastname: Fernández-Astorga
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002169:Campylobacter Infections / Q000382:microbiology; D016123:Campylobacter jejuni / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; C067044:cytolethal distending toxin