Determination of vincristine in infant plasma by liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy.
An LC-MS method using APCI has been developed and validated for the determination of the anticancer drug vincristine in human plasma, using vinblastine as internal standard. Following solid-phase extraction (SPE) of the sample, the lower limit of quantitation (LLOQ) was 0.18 ng/ml, the lower limit of detection was 0.09 ng/ml, and the linear calibration range was 0.18-180 ng/ml. This method has been used to measure plasma concentrations of vincristine from 0.08 to 24 h post bolus in 29 infants as part of a pharmacokinetic study. Concentrations of vincristine at 24 h were 0.2-1.36 ng/ml.
Schmidt
Mark S
MS
College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA.
Huang
Rong
R
Classon
Robert J
RJ
Murry
Daryl J
DJ
D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D014747:Vinblastine / Q000031:analogs & derivatives / Q000097:blood; D014750:Vincristine / Q000097:blood* / Q000493:pharmacokinetics; D000077235:Vinorelbine
D000972:Antineoplastic Agents, Phytogenic; D014750:Vincristine; D014747:Vinblastine; D000077235:Vinorelbine