title: Passive immunization using purified IgYs against infectious bursal disease of chickens in Pakistan.
abstract: Infectious bursal disease (IBD) is an acute and highly contagious disease of young chickens caused by Birnavirus. Mortality of infected birds can be best prevented if injected with antibodies. The present study was an attempt to raise specific hyper-immune polyclonal antibodies against IBD virus in Pakistan. Commercial layers divided into four groups were injected with IBD vaccine subcutaneously according to four different treatment regimens. Eggs were collected daily and antibodies were purified from yolk with dextran sulphate. Titers of antibodies in serum and yolk were evaluated with enzyme linked immunosorbant assay and agar gel precipitation test. Antibody titers were significantly higher in yolk than serum. Eggs collected at 28 days post-vaccination had maximum antibody titers. Of treatment regimens, T3 was found to be most effective for hyperimmunization. Lyophilized antibodies stored at 4 degrees C did not lose their activity till the end of experiment. IBD virus infected birds were injected with purified antibodies which induced 92% recovery as compared to control birds. The study implicates that the purified antibodies may be useful as a therapeutic agent to cure IBD infected birds.
authors.affiliation: Department of Biological Sciences, Quaid-i-Azam University, Islamabad P.O. Box 45320, Pakistan.
authors.forename: Muhammad Wasif
authors.initials: MW
authors.lastname: Malik
authors.forename: Najma
authors.initials: N
authors.lastname: Ayub
authors.forename: Irfan Zia
authors.initials: IZ
authors.lastname: Qureshi
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018175:Birnaviridae Infections / Q000276:immunology / Q000628:therapy* / Q000662:veterinary* / Q000821:virology; D002645:Chickens; D004530:Egg Yolk / Q000276:immunology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007114:Immunization / Q000379:methods / Q000662:veterinary*; D007136:Immunoglobulins / Q000276:immunology*; D007167:Immunotherapy / Q000379:methods / Q000662:veterinary; D007243:Infectious bursal disease virus / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology / Q000628:therapy* / Q000821:virology*; D011233:Precipitin Tests / Q000662:veterinary; D014765:Viral Vaccines / Q000276:immunology* / Q000627:therapeutic use
chemical_list: D000914:Antibodies, Viral; C016512:IgY; D007136:Immunoglobulins; D014765:Viral Vaccines