Efficient resolution of Pneumocystis murina infection in surfactant protein A-deficient mice following withdrawal of corticosteroid-induced immunosuppression.
Following withdrawal of immunosuppression, surfactant protein A (SP-A)-deficient and wild-type mice cleared Pneumocystis murina infection in a similar manner, but exhibited significant differences in lymphocyte populations, interleukin (IL)-6 levels and chemokine expression levels. A higher percentage of lymphocytes were detected in lung lavage fluid from SP-A-deficient mice, but more CD4+ T cells were isolated from lung tissue of wild-type mice. Higher concentrations of IL-6 were detected in lavage fluid and enhanced expression of lymphotactin and RANTES were detected in the lungs of wild-type mice. Equal levels of surfactant protein D were detected in SP-A-deficient and wild-type mice and no differences were detected in markers of lung injury between the two strains of mice. Thus, SP-A does not enhance organism clearance, but does modulate the host immune response during resolution of P. murina infection.
Linke
Michael
M
University of Cincinnati College of Medicine, Department of Internal Medicine, Division of Infectious Diseases, 231 Bethesda, Cincinnati, OH 45221, USA.|Department of Veterans Affairs Medical Center, Research Service, 3200 Vine St, Cincinnati, OH 45220, USA.
Ashbaugh
Alan
A
University of Cincinnati College of Medicine, Department of Internal Medicine, Division of Infectious Diseases, 231 Bethesda, Cincinnati, OH 45221, USA.
Koch
Judith
J
University of Cincinnati College of Medicine, Department of Internal Medicine, Division of Infectious Diseases, 231 Bethesda, Cincinnati, OH 45221, USA.
Tanaka
Reiko
R
University of Cincinnati College of Medicine, Department of Internal Medicine, Division of Infectious Diseases, 231 Bethesda, Cincinnati, OH 45221, USA.
Walzer
Peter
P
University of Cincinnati College of Medicine, Department of Internal Medicine, Division of Infectious Diseases, 231 Bethesda, Cincinnati, OH 45221, USA.|Department of Veterans Affairs Medical Center, Research Service, 3200 Vine St, Cincinnati, OH 45220, USA.
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002452:Cell Count; D018946:Chemokine CCL5 / Q000032:analysis; D003907:Dexamethasone / Q000008:administration & dosage; D016867:Immunocompromised Host; D015850:Interleukin-6 / Q000032:analysis; D008168:Lung / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008222:Lymphokines / Q000032:analysis; D051379:Mice; D008809:Mice, Inbred C3H; D018345:Mice, Knockout; D011010:Pneumocystis; D011020:Pneumonia, Pneumocystis / Q000276:immunology*; D037662:Pulmonary Surfactant-Associated Protein A / Q000172:deficiency* / Q000235:genetics*; D012795:Sialoglycoproteins / Q000032:analysis
D018946:Chemokine CCL5; D015850:Interleukin-6; D008222:Lymphokines; D037662:Pulmonary Surfactant-Associated Protein A; D012795:Sialoglycoproteins; C018997:lymphotactin; D003907:Dexamethasone