title: Analysis of human plasma proteome by 2DE- and 2D nanoLC-based mass spectrometry.
abstract: We compared the 2DE coupled to MALDI-TOF-MS and ESI-MS/MS analysis (2DE-MS) and the on-line 2D nanoLC, followed by nanoESI-MS/MS analysis (2DLC-MS), for the separation and identification of proteins in high abundance protein-depleted human plasma. Identification of proteins in the plasma by the two methods demonstrated that the majority of the identified protein set was unique to each method. Therefore, if a comprehensive coverage of the proteome identification is desired, it is ideal to apply both methods. The 2DE-MS method is amenable to protein spot-based quantitation, whereas the 2DLC-MS method may provide an advantage of the high throughput application.
authors.affiliation: Department of Cellular Stress Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
authors.forename: Kyoung-Soo
authors.initials: KS
authors.lastname: Choi
authors.forename: Liguo
authors.initials: L
authors.lastname: Song
authors.forename: Young-Mee
authors.initials: YM
authors.lastname: Park
authors.forename: James
authors.initials: J
authors.lastname: Marshall
authors.forename: Anders L
authors.initials: AL
authors.lastname: Lund
authors.forename: Henry
authors.initials: H
authors.lastname: Shion
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Park
authors.forename: Ho-Zoon
authors.initials: HZ
authors.lastname: Chae
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Park
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001798:Blood Proteins; D020543:Proteome