Hepatocyte growth factor-regulated genes in differentiated RAW 264.7 osteoclast and undifferentiated cells.
Hepatocyte Growth Factor (HGF) and its protooncogene receptor c-Met regulate osteoclast function by activating pp60(c-Src) kinase and alpha(v)beta3 integrin. HGF causes transcription yet in osteoclast cells, this gene regulation is currently unknown. To begin characterization of HGF-regulated gene expression in osteoclast cells, we used a well characterized model of osteoclast cells. Using microarray, relative RT-PCR, and Western blot analyses, we have identified and confirmed differentially expressed genes in RAW 264.7 osteoclast cells in response to HGF. HGF regulation of transcription of these genes was concordant with microarray results. We report that HGF downregulates transcription factors, Distal-less 5 (Dlx-5), Distal-less 6 (Dlx-6) and Aristaless 4 (Alx-4), in RAW 264.7 osteoclast cells but has an inverse effect in undifferentiated RAW 264.7 cells.
Gaasch
Julie A
JA
Department of Pharmaceutical Sciences, Texas Tech University Health Science Center School of Pharmacy, Amarillo, TX 79106, USA.
Bolwahnn
Alanna B
AB
Lindsey
J Suzanne
JS
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002454:Cell Differentiation; D002460:Cell Line; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000502:physiology*; D005801:Genes, Homeobox; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics; D017228:Hepatocyte Growth Factor / Q000502:physiology*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; C494642:Dnajb3 protein, mouse; D050956:HSP40 Heat-Shock Proteins; D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met