Antioxidant activity of novel chitin derivative.
Novel water-soluble chitin derivative was prepared by chemical modification to evaluate antioxidant activities by free radical scavenging potential using electron spin resonance spin trapping technique. Aminoethyl-chitin (AEC) exhibited free radical scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, superoxide, and peroxyl radicals. AEC quenched DPPH and peroxyl radical over 55% and 59% at 4mg/mL, and also suppressed superoixde radical over 58% at 2mg/mL. Especially, AEC was more active against hydroxyl radical, and scavenging ratio was 92.2% at 0.12mg/mL. These results suggested that free amino group in the -CH(2)CH(2)NH(2) plays an important role in the free radical scavenging activity. In addition, cytotoxic effect of AEC was assessed using human lung fibroblast (MRC-5) cell line, and AEC showed less toxic against MRC-5.
Je
Jae-Young
JY
Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
Kim
Se-Kwon
SK
D000975:Antioxidants / Q000494:pharmacology*; D002686:Chitin / Q000737:chemistry / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D004797:Enzyme-Linked Immunosorbent Assay; D009682:Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared
D000975:Antioxidants; D002686:Chitin