title: Expression of the Gas7 gene and Oct4 in embryonic stem cells of mice.
abstract: Oct4 is a stem cell expression marker, and persistence of its expression retains pluripotency in embryonic stem (ES) cells. Our results indicate a pattern of gradual decrease in Oct4 expression, which is prominent in the blastocyst and markedly reduced in the gastrula and neurula. The presence of POU transcription factor-like domain in Gas7 prompted us to look for its expression in the early embryonic cells. We have observed high expression of the Gas7 protein in blastocysts that gradually decreased in the neurula stages. The localization of Gas7 was initially seen throughout the blastocyst, but was later confined to dorsal ectodermal regions of the neurula, conforming with its role in neuronal differentiation. Our data reveal that Gas7 might play a role in cellular migration and cell protection in the ES cells of mouse embryo.
authors.affiliation: Unit of Biochemistry, Department of Zoology, University of Madras, Chennai-600 025, Tamil Nadu, India.
authors.forename: P P
authors.initials: PP
authors.lastname: Moorthy
authors.forename: A Anand
authors.initials: AA
authors.lastname: Kumar
authors.forename: H
authors.initials: H
authors.lastname: Devaraj
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002454:Cell Differentiation; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D047108:Embryonic Development; D005775:Gastrula / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism*; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D013234:Stem Cells / Q000502:physiology*
chemical_list: C485435:Gas7 protein, mouse; D009419:Nerve Tissue Proteins; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D020033:Protein Isoforms