title: Protein microarrays: a new tool for profiling antibody cross-reactivity.
abstract: Antibody cross-reactivity can compromise interpretation of experiments and derail therapeutic antibody development. Standard techniques such as immunohistochemistry or Western analysis provide important but often inadequate approaches to assess antibody specificity. Protein microarrays are providing a new approach to rapidly characterize antibody cross-reactivity against 1,000s of proteins simultaneously. This review will focus on reported examples of antibody cross-reactivity, methods used to characterize them, and the recent development and use of protein microarrays for assessing antibody specificity.
authors.affiliation: Invitrogen Protein Microarray Center, 688 E Main St, Branford, CT 06405, USA. paul.predki@invitrogen.com
authors.forename: Paul F
authors.initials: PF
authors.lastname: Predki
authors.forename: Dawn
authors.initials: D
authors.lastname: Mattoon
authors.forename: Rhonda
authors.initials: R
authors.lastname: Bangham
authors.forename: Barry
authors.initials: B
authors.lastname: Schweitzer
authors.forename: Gregory
authors.initials: G
authors.lastname: Michaud
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D040081:Protein Array Analysis