Influence of seasons, extenders, slow and rapid freezing on seminal characters in Korean native bucks.
The success of AI technology is based on both semen quality and freezing process. In order to establish the semen freezing techniques in Korean native bucks, factors affecting the success were evaluated in the present study. Semen collected by electro-ejaculation from bucks during four distinct seasons was evaluated for semen volume and pH, sperm motility and survivability. The semen volume, concentration and total cell were higher in spring, summer and less in winter. Yet, there were no seasonal differences in the proportional data of live sperm, motility score and pH of semen among seasons. The percentage of live sperm after thawing was found to increase with increased concentration of lactose in Tris-Egg yolk-glycerol (TY-G), being highest in TY-G supplemented with 180 mm lactose (TYL180-G), but did not differ between TY-G and TYL120-G. Sperm motility was enhanced by employing 2.0 h equilibration time with rapid freezing method. In conclusion, semen could be frozen with high success rates for further use of AI in breeding techniques and to preserve the Korean native bucks.
Choe
Chang-Yong
CY
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Gazwa, Chinju, Republic of Korea.
Kim
Jung-Gon
JG
Cho
Sang-Rae
SR
Son
Dong-Soo
DS
Kim
Young-Keun
YK
Balasubramanian
S
S
Choe
Sang-Yong
SY
Rho
Gyu-Jin
GJ
D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D006041:Goats / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D012621:Seasons; D012662:Semen Preservation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D013076:Sperm Count / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology*; D013997:Time Factors
D003451:Cryoprotective Agents