title: Association of antipituitary antibody and type 2 iodothyronine deiodinase antibody in patients with autoimmune thyroid disease.
abstract: Antipituitary antibody (APA) has been reported to be detected in patients with autoimmune thyroid disease. Type 2 iodothyronine deiodinase (D2) is expressed in both pituitary gland and thyroid gland. We studied the association of APA and D2 peptide antibody in patients with autoimmune thyroid disease. Rat pituitary gland homogenate and D2 peptide were used as antigens in the present study. APA and D2 peptide antibodies were measured by enzyme-linked immunosorbent assay (ELISA) in sera obtained from 42 patients with Hashimoto's disease, 26 patients with Graves' disease and 70 healthy control subjects. Moreover, D2 activity precipitation assay was performed in some patients with Hashimoto's disease. APA and D2 peptide antibody were elevated in patients with Hashimoto's disease and patients with Graves' disease, compared with control subjects. APA was positive in 32.4% (22/68), D2 peptide antibody was positive in 26.5% (18/68) of patients with autoimmune thyroid disease. APA was positive in 31.0% (13/42) of patients with Hashimoto's disease and 34.6% (9/26) of patients with Graves' disease. D2 peptide antibody was positive in 26.2% (11/42) of patients with Hashimoto's disease and 26.9% (7/26) of patients with Graves' disease. D2 peptide antibody was correlated with APA in patients with autoimmune thyroid disease. Moreover, precipitation of D2 activity was increased in some patients with Hashimoto's disease including a patient who also had idiopathic diabetes insipidus, and was correlated with D2 peptide antibody. These results suggest that D2 antibody may be associated with APA in patients with autoimmune thyroid disease.
authors.affiliation: Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi.
authors.forename: Rieko
authors.initials: R
authors.lastname: Nakahara
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Tsunekawa
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yabe
authors.forename: Misa
authors.initials: M
authors.lastname: Nara
authors.forename: Koji
authors.initials: K
authors.lastname: Seki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kasahara
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ogiwara
authors.forename: Michio
authors.initials: M
authors.lastname: Nishino
authors.forename: Isao
authors.initials: I
authors.lastname: Kobayashi
authors.forename: Masami
authors.initials: M
authors.lastname: Murakami
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006111:Graves Disease / Q000097:blood / Q000276:immunology*; D050031:Hashimoto Disease / Q000097:blood / Q000276:immunology*; D006801:Humans; D047468:Immunoprecipitation; D007453:Iodide Peroxidase / Q000032:analysis / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010902:Pituitary Gland / Q000201:enzymology / Q000276:immunology*; D013961:Thyroid Gland / Q000201:enzymology / Q000276:immunology*; D000097564:Iodothyronine Deiodinase Type II
chemical_list: D001323:Autoantibodies; D007453:Iodide Peroxidase; D000097564:Iodothyronine Deiodinase Type II