Cell dynamics in the correct control of bone metabolism using natural treatments.
The present study was undertaken in order to assess the efficacy of a commercial product containing calcium and silicon (Osteosil-Calcium) on cell metabolism. MG-63 osteblast-like cells were cultured in the presence of three different drug concentrations (10, 5 and 2.5 microg/mL). Either serum-free culture and standard culture with serum were investigated. Morpho-functional tests (MTT and ALP), scanning electron microscopy (SEM), microanalysis (EDAX) and time-lapse video microscopy were performed. Cell actin cytoskeletal modification with fluorescence phalloidin staining was also tested. Our data show the in vitro functional efficacy of Osteosil-Calcium on MG63 cell viability and ALP production. This study demonstrates its positive effect on the metabolism of the single cell and suggests wider uses of this drug in health protection and or in Regenerative Medicine therapies which are currently applied to the elderly.
Mattioli-Belmonte
M
M
Institute of Normal Human Morphology, Universitàa'Politecnica delle Marche, Ancona, Italy. m.mattioli@univpm.it
Kyriakidou
K
K
Lucarini
G
G
Gorrieri
O
O
Giavaresi
G
G
Fini
M
M
Giardino
R
R
Amati
S
S
Suffritti
G
G
Biagini
G
G
D000704:Analysis of Variance; D002118:Calcium / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D010006:Osteoblasts / Q000187:drug effects* / Q000648:ultrastructure; D012825:Silicon / Q000494:pharmacology*; D013997:Time Factors
D002118:Calcium; D012825:Silicon