[RNA isolation from human embryonic tissues].
To investigate the method of RNA isolation from human embryonic tissues and the factors influencing the quality of RNA, the RNA from human embryonic tissues obtained with drug-induced labor or non-drug induced labor were isolated by using grind with liquid nitrogen or homogenizer without liquid nitrogen. The results showed that the positive rates of RNA integrity in grind with liquid nitrogen group and in homogenizer without liquid nitrogen group were 68.42% and 29.79% respectively, and there was significant difference between these two groups; however, there was no statistic difference in positive rate of RNA integrity, OD(260)/OD(280) ratio and beta-actin gene expression level between the drug-induced labor group and non-drug induced labor group. It is concluded that pulverize of tissue in liquid nitrogen remains the integrity of RNA isolated and may be applied for RNA isolation from human embryonic tissues. The quality of RNA is not affected by different methods of induction of maternal labor.
He
Ying-Yi
YY
Department of Pediatrics, The First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, China.
He
Xin-Rong
XR
Ye
Tie-Zhen
TZ
D004622:Embryo, Mammalian / Q000378:metabolism*; D005615:Freezing; D006801:Humans; D009584:Nitrogen / Q000494:pharmacology; D012313:RNA / Q000302:isolation & purification*; D020871:RNA Stability / Q000187:drug effects
D012313:RNA; D009584:Nitrogen