Biochemical stress response in freshwater fish Channa punctatus induced by aqueous extracts of Euphorbia tirucalli plant.
Piscicidal activities of aqueous extracts of Euphorbia tirucalli were very well established, but their ultimate mode of action on fish metabolism was not yet known. Exposure of fishes over 24h or 96h to sub-lethal doses (40% and 80% of LC(50)) of aqueous extract of E. tirucalli stem-bark and latex, significantly (P<0.05) altered the level of total protein, total free amino acids, nucleic acids, glycogen, pyruvate, lactate and activity of protease, alanine aminotransferase, aspartate aminotransferase, acetylcholinesterase and cytochrome oxidase enzyme in liver and muscle tissues of freshwater fish Channa punctatus. The alterations in all these biochemical parameters were significantly (P<0.05) time- and dose-dependent. After 7d of withdrawal of treatment of 80% of LC(50) of E. tirucalli extracts shows that there was a partial recovery in the levels of glycogen but nearly complete recovery in total protein, total free amino acids, pyruvate, lactate, nucleic acids level and activity of protease, aspartate aminotransferase, alanine aminotransferase, acetylcholinesterase and cytochrome oxidase enzyme in both the tissues of fish. Thus aqueous extracts of E. tirucalli adversely affect respiratory pathway of fish and cause energy crisis during stress by suppressing ATP production. The reversibility of the action of the aqueous extracts would be an additional advantage in their use.
Tiwari
Sudhanshu
S
Natural Products Laboratory, Research Block, Department of Zoology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur 273009, Uttar Pradesh, India. sudhansh4@rediffmail.com
Singh
Ajay
A
D000110:Acetylcholinesterase / Q000378:metabolism; D000410:Alanine Transaminase / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D004247:DNA / Q000032:analysis; D003576:Electron Transport Complex IV / Q000378:metabolism; D028481:Euphorbia / Q000737:chemistry*; D006003:Glycogen / Q000032:analysis; D019344:Lactic Acid / Q000032:analysis; D007840:Latex / Q000737:chemistry; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010473:Perciformes / Q000378:metabolism*; D010936:Plant Extracts / Q000633:toxicity*; D018547:Plant Stems / Q000737:chemistry; D011506:Proteins / Q000032:analysis; D019289:Pyruvic Acid / Q000032:analysis; D012313:RNA / Q000032:analysis; D014867:Water / Q000737:chemistry
D000596:Amino Acids; D007840:Latex; D010936:Plant Extracts; D011506:Proteins; D014867:Water; D019344:Lactic Acid; D012313:RNA; D019289:Pyruvic Acid; D006003:Glycogen; D004247:DNA; D003576:Electron Transport Complex IV; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000110:Acetylcholinesterase; D010447:Peptide Hydrolases