title: Chromokinesin Xklp1 contributes to the regulation of microtubule density and organization during spindle assembly.
abstract: Xklp1 is a chromosome-associated kinesin required for Xenopus early embryonic cell division. Function blocking experiments in Xenopus egg extracts suggested that it is required for spindle assembly. We have reinvestigated Xklp1 function(s) by monitoring spindle assembly and microtubule behavior under a range of Xklp1 concentrations in egg extracts. We found that in the absence of Xklp1, bipolar spindles form with a reduced efficiency and display abnormalities associated with an increased microtubule mass. Likewise, centrosomal asters assembled in Xklp1-depleted extract show an increased microtubule mass. Conversely, addition of recombinant Xklp1 to the extract reduces the microtubule mass associated with spindles and asters. Our data suggest that Xklp1 affects microtubule polymerization during M-phase. We propose that these attributes, combined with Xklp1 plus-end directed motility, contribute to the assembly of a functional bipolar spindle.
authors.affiliation: Cell Biology and Biophysics Program, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.
authors.forename: Mirco
authors.initials: M
authors.lastname: Castoldi
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vernos
mesh_terms: D000818:Animals; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D016547:Kinesins / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000172:deficiency / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D010063:Ovum / Q000166:cytology; D011108:Polymers; D011994:Recombinant Proteins / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism; D029867:Xenopus Proteins / Q000172:deficiency / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D008869:Microtubule-Associated Proteins; C452196:NAT14 protein, Xenopus; D009687:Nuclear Proteins; D011108:Polymers; D011994:Recombinant Proteins; D014404:Tubulin; D029867:Xenopus Proteins; C092349:chromokinesin; D004247:DNA; D016547:Kinesins