[Inhibiting effects of recipe for dispersing stagnated liver qi to promote bile flow on cholesterol gallstone formation in guinea pigs].
OBJECTIVE
To explore the mechanism of the inhibiting effects of recipe for dispersing stagnated liver qi to promote bile flow (Danshijing Tablets) on cholesterol gallstone formation and provide experimental evidence for its clinical application.


METHODS
Eighty guinea pigs were randomly divided into 4 groups, which were normal control group, untreated group, ursodeoxycholic acid (UDCA)-treated group and Danshijing Tablets-treated group. The gallstones in guinea pigs were induced by high-cholesterol diet. UDCA and Danshijing Tablets were given orally to guinea pigs in the corresponding group respectively for seven weeks. Then the physical signs of the guinea pigs, the rates of gallstone formation and the histomorphological changes of the gallbladder were examined.


RESULTS
The behavior of guinea pigs in the Danshijing Tablets-treated group was obviously improved and the rate of gallstone formation was significantly decreased as compared with those in both untreated and UDCA-treated groups (P<0.05). The inflammation reaction of mucous membrane in gallbladder was evidently reduced in the Danshijing Tablets-treated group and its morphological appearance turned to be approximately normal.


CONCLUSIONS
Recipe for dispersing stagnated liver qi to promote bile flow may decrease gallstone formation. Its mechanism may be related to reducing pathologic changes in gallbladder tissues, which will reduce the damages of cholesterol to the smooth muscle in gallbladder and enhance the contractility of gallbladder.
Fang
Bang-Jiang
BJ
Institute of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032. fangbji@163.com
Zhu
Pei-Ting
PT
Zhang
Jing-Zhe
JZ
Pei
Xin-Jun
XJ
Yang
Ying-Xin
YX
Liang
Xiao-Qiang
XQ
D000818:Animals; D002756:Cholagogues and Choleretics / Q000627:therapeutic use*; D002769:Cholelithiasis / Q000737:chemistry / Q000517:prevention & control*; D002784:Cholesterol; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D005704:Gallbladder / Q000187:drug effects; D016473:Gallbladder Emptying / Q000187:drug effects; D006168:Guinea Pigs; D008297:Male; D008517:Phytotherapy; D011897:Random Allocation; D013607:Tablets
D002756:Cholagogues and Choleretics; D004365:Drugs, Chinese Herbal; D013607:Tablets; D002784:Cholesterol