Allele-specific deposition of macroH2A1 in imprinting control regions.
In the current study, we analyzed the deposition patterns of macroH2A1 at a number of different genomic loci located in X chromosome and autosomes. MacroH2A1 is preferentially deposited at methylated CpG-rich regions located close to promoters. The macroH2A1 deposition patterns at the methylated CpG islands of several imprinted domains, including the imprinting control regions (ICRs) of Xist, Peg3, H19/Igf2, Gtl2/Dlk1 and Gnas domains, show consistent allele-specificity towards inactive, methylated alleles. The macroH2A1 deposition levels at the ICRs and other differentially methylated regions of these domains are also either higher or comparable to those observed at the inactive X chromosome of female mammals. Overall, our results indicate that besides DNA methylation macroH2A1 is another epigenetic component in the chromatin of ICRs displaying differential association with two parental alleles.
Choo
Jung Ha
JH
Department of Biological Sciences, Center for BioModular Multi-scale Systems, Louisiana State University, Baton Rouge, LA 70803, USA.
Kim
Jeong Do
JD
Chung
Jae Hoon
JH
Stubbs
Lisa
L
Kim
Joomyeong
J
D000483:Alleles; D000818:Animals; D047369:Chromatin Immunoprecipitation; D018899:CpG Islands; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D018392:Genomic Imprinting; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014960:X Chromosome
D006657:Histones