title: Functional mapping in pea, as an aid to the candidate gene selection and for investigating synteny with the model legume Medicago truncatula.
abstract: The identification of the molecular polymorphisms giving rise to phenotypic trait variability-both quantitative and qualitative-is a major goal of the present agronomic research. Various approaches such as positional cloning or transposon tagging, as well as the candidate gene strategy have been used to discover the genes underlying this variation in plants. The construction of functional maps, i.e. composed of genes of known function, is an important component of the candidate gene approach. In the present paper we report the development of 63 single nucleotide polymorphism markers and 15 single-stranded conformation polymorphism markers for genes encoding enzymes mainly involved in primary metabolism, and their genetic mapping on a composite map using two pea recombinant inbred line populations. The complete genetic map covers 1,458 cM and comprises 363 loci, including a total of 111 gene-anchored markers: 77 gene-anchored markers described in this study, 7 microsatellites located in gene sequences, 16 flowering time genes, the Tri gene, 5 morphological markers, and 5 other genes. The mean spacing between adjacent markers is 4 cM and 90% of the markers are closer than 10 cM to their neighbours. We also report the genetic mapping of 21 of these genes in Medicago truncatula and add 41 new links between the pea and M. truncatula maps. We discuss the use of this new composite functional map for future candidate gene approaches in pea.
authors.affiliation: Unité de recherche en génétique et écophysiologie des légumineuses, URLEG, INRA, Domaine d'Epoisses, 21110 Bretenières, France.
authors.forename: G
authors.initials: G
authors.lastname: Aubert
authors.forename: J
authors.initials: J
authors.lastname: Morin
authors.forename: F
authors.initials: F
authors.lastname: Jacquin
authors.forename: K
authors.initials: K
authors.lastname: Loridon
authors.forename: M C
authors.initials: MC
authors.lastname: Quillet
authors.forename: A
authors.initials: A
authors.lastname: Petit
authors.forename: C
authors.initials: C
authors.lastname: Rameau
authors.forename: I
authors.initials: I
authors.lastname: Lejeune-Hénaut
authors.forename: T
authors.initials: T
authors.lastname: Huguet
authors.forename: J
authors.initials: J
authors.lastname: Burstin
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics; D030541:Databases, Genetic; D035264:Flowers / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D046913:Medicago truncatula / Q000235:genetics* / Q000254:growth & development; D018895:Microsatellite Repeats; D008954:Models, Biological; D018532:Pisum sativum / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D012641:Selection, Genetic; D026801:Synteny
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers