Detection of cytotoxic activity of Psacalium palladium on normal mouse spleen cells and transformed human cells.
A decoction from roots and rhizomes of Psacalium palladium is used in Mexico for the treatment of tumors and for control of diabetes mellitus, among other ailments. Although hypoglycemic activity has been demonstrated in various experimental models and some compounds have been detected in the roots and rhizomes, such as alkaloids, glycosides and tannins, toxicity studies have not yet been performed. Moreover, since various species taxonomically related to P. palladium have pyrrolizidine alkaloids (hepatotoxic, carcinogenic and mutagenic agents) it is possible that these species also contain potentially toxic substances, which represents a risk for users. The aim of this investigation was study the cytotoxicity of P. palladium on mouse hematopoietic cells and transformed human cells, evaluating the cell proliferation by the technique of sulforhodamine B. An aqueous fraction (AS) reduced population of both types of cells by over 50%; meanwhile the other extracts did not alter cell number. Fraction AP was cytotoxic for DU-145 cells at 10 microg/mL, whereas the remaining extracts acted like cytostatic agents.
Velasco
Lezama Rodolfo
LR
Laboratorio de Hematología, Universidad Autonoma Metropolitana-Iztapalapa, México. velr@xanum.uam.mx
Pérez
Julio Cesar Almanza
JC
Aguilar
Rafaela Tapia
RT
Román Ramos
Rubén
R
Carrillo
Laura Vázquez
LV
Alarcón Aguilar
Francisco
F
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000473:pathology; D013154:Spleen / Q000166:cytology* / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts