title: Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells.
abstract: The damaged DNA-binding protein (DDB) complex consists of a heterodimer of p127 (DDB1) and p48 (DDB2) subunits and is believed to have a role in nucleotide excision repair (NER). We used the GAL4-UAS targeted expression system to knock down DDB1 in wing imaginal discs of Drosophila. The knock-down was achieved in transgenic flies using over-expression of inverted repeat RNA of the D-DDB1 gene [UAS-D-DDB1(650)-dsRNA]. As a consequence of RNA interference (RNAi), the fly had a shrunken wing phenotype. The wing spot test showed induced genome instability in transgenic flies with RNAi knock-down of D-DDB1 in wing imaginal discs. When Drosophila larvae with RNAi knock-down of D-DDB1 in wing imaginal discs were treated with the chemical mutagen methyl methanesulfonate (MMS), the frequency of flies with a severely shrunken wing phenotype increased compared to non-treated transgenic flies. These results suggested that DDB1 plays a role in the response to DNA damaged with MMS and in genome stability in Drosophila somatic cells.
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510.
authors.forename: Kaori
authors.initials: K
authors.lastname: Shimanouchi
authors.forename: Kei-Ichi
authors.initials: K
authors.lastname: Takata
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Shizuka
authors.initials: S
authors.lastname: Murakami
authors.forename: Gen
authors.initials: G
authors.lastname: Ishikawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Takeuchi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kanai
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Ruike
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Nakamura
authors.forename: Yoko
authors.initials: Y
authors.lastname: Abe
authors.forename: Kengo
authors.initials: K
authors.lastname: Sakaguchi
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000502:physiology*; D004330:Drosophila; D005260:Female; D042822:Genomic Instability; D008297:Male; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D004268:DNA-Binding Proteins