Methylobacter tundripaludum sp. nov., a methane-oxidizing bacterium from Arctic wetland soil on the Svalbard islands, Norway (78 degrees N).
A Gram-negative, rod-shaped, non-motile, non-spore forming bacterium (SV96T) was isolated from wetland soil near Ny-Alesund, Svalbard. On the basis of 16S rRNA gene sequence similarity, strain SV96T was shown to belong to the Gammaproteobacteria, related to Methylobacter psychrophilus Z-0021T (99.1 %), Methylobacter luteus ATCC 49878T (97.3 %), Methylobacter marinus A45T (97.0 %) and Methylobacter whittenburyi ATCC 51738T (95.8 %); the closest related species within the genus Methylomicrobium with a validly published name was Methylomicrobium album ATCC 33003T (95.0 %). Chemotaxonomic data (including the major fatty acids: 16 : 1omega8, 16 : 1omega7 and 16 : 1omega5t) supported the affiliation of strain SV96T to the genus Methylobacter. The results of DNA-DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain SV96T from the four Methylobacter species mentioned above. Strain SV96T therefore represents a novel species, for which the name Methylobacter tundripaludum sp. nov. is proposed (type strain SV96T = DSM 17260T = ATCC BAA-1195T).
Wartiainen
Ingvild
I
Department of Biology, Faculty of Science, University of Tromsø, N-9037 Tromsø, Norway.
Hestnes
Anne Grethe
AG
McDonald
Ian R
IR
Svenning
Mette M
MM
D001110:Arctic Regions; D005227:Fatty Acids; D008772:Methylococcaceae / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D009664:Norway; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013045:Species Specificity
D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S