title: Calorimetric analysis of thermodynamic stability and aggregation for apo and holo amyotrophic lateral sclerosis-associated Gly-93 mutants of superoxide dismutase.
abstract: Differential scanning calorimetry was used to measure changes in thermodynamic stability and aggregation for glycine 93 mutants of human copper, zinc-superoxide dismutase (SOD). Glycine 93 is a conserved residue at position i + 3 of a tight turn and has been found to be a mutational hot spot in familial amyotrophic lateral sclerosis (fALS). The fALS-associated mutations, G93A, G93S, G93R, G93D, and G93V, were made in a pseudo wild-type background containing no free cysteines, which prevented the formation of aberrant disulfide bonds upon thermal unfolding, and enabled quantitative thermodynamic analysis of the effects of the mutations. Thermal unfolding was highly reversible for all the SODs in both the fully metallated (holo) and metal-free (apo) forms. The data for all the holo-SODs and for the apo-pseudo-wild-type SOD were well fit by a 2-state unfolding model for native dimer (N2) to two unfolded monomers (2U), N2 <--> 2U. The holo- and apo-forms of the mutants are significantly destabilized (by 1.5-3.5 kcal mol(-1) monomer) relative to the corresponding forms of pseudo wild-type, with the relative stabilities being correlated with statistical preferences for amino acids in this structural context. Although van't Hoff (DeltaHvH) to calorimetric (DeltaHcal) enthalpy ratios are close to unity for all the holo-SODs and for apo-pseudo-wild-type, consistent with a 2-state transition, DeltaHvH is considerably larger than DeltaHcal for all the apo-mutants. This suggests that the mutations cause apo-SOD to have an increased propensity to misfold or aggregate, which may be linked to increased toxic mutant SOD aggregation in fALS.
authors.affiliation: Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Stathopulos
authors.forename: Jessica A O
authors.initials: JA
authors.lastname: Rumfeldt
authors.forename: Farhad
authors.initials: F
authors.lastname: Karbassi
authors.forename: Clare A
authors.initials: CA
authors.lastname: Siddall
authors.forename: James R
authors.initials: JR
authors.lastname: Lepock
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Meiering
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics*; D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics* / Q000378:metabolism*; D001051:Apoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D019281:Dimerization; D004795:Enzyme Stability / Q000235:genetics; D005998:Glycine / Q000235:genetics* / Q000378:metabolism; D020035:Holoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008027:Light; D011489:Protein Denaturation; D017510:Protein Folding; D012542:Scattering, Radiation; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D001051:Apoenzymes; D020035:Holoenzymes; D013482:Superoxide Dismutase; D005998:Glycine