[A method for Rhesus box test].
To study the method for Rhesus box test and its significance, the sequence specific primers of upstream, downstream and hybrid Rhesus boxes were designed according to RhD gene sequence; the upstream, downstream and hybrid Rhesus boxes were determined by PCP-SSP and mismatched PCR. The results showed that this method was confirmed by DNA Standard test. It was shown that in unrelative RhD positive individuals RHD(+)/RHD(-), RHD(+)/RHD(+) genotype accounted for 9.00%, 91.00% respectively, and in RhD negative individuals RHD(+)/RHD(-), RHD(+)/RHD(+), RHD(-)/RHD(-) genotype were 26.14%, 3.92%, 69.94% respectively. It is concluded that the method of Rhesus box test was confirmed to be reliable and can be used for the identification of RhD haplotype gene structure, as well as for study on inheritance, clinical transfusion and neonatal hemolytic diseases.
Lan
Jiong-Cai
JC
Department of Blood Transfusion, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. gzlanjc@tom.com
Zhou
Hua-You
HY
Xia
Rong
R
Cao
Qiong
Q
Xing
Yan-Chao
YC
Pang
Gui-Zhi
GZ
Wu
Can
C
Yang
Quan-Ke
QK
D001483:Base Sequence; D006239:Haplotypes; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012204:Rh-Hr Blood-Group System / Q000032:analysis / Q000235:genetics*
D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen