Dynamic process of phospholipid-protein interaction studied by capillary isoelectric focusing with whole-column imaging detection.
Liposomes are vesicles formed by the aggregation of amphiphilic phospholipid molecules, which can mimic natural cell membranes. Interaction between liposome and protein is important for the structure and function of natural cell membranes. In this study, a CIEF method with whole-column imaging detection was developed for monitoring the dynamic process of phospholipid-protein interactions. The CIEF profiles at successive interaction times clearly displayed the formation of the different conjugates between phospholipid and protein at different stages. Due to the diversity of the chemical and physical properties of targeted proteins in this study (trypsin inhibitor, beta-lactoglobulin B, phosphorylase b, and trypsinogen), different dynamic processes of phospholipid-protein interactions were exhibited. The type of phospholipids played an important role in the dynamic process of phospholipid-protein interaction, as noted by the use of zwitterionic phospholipid (phosphatidylcholine) and acidic phospholipid (phosphatidylserine). Mechanisms involved in these interactions were discussed by monitoring the dynamic processes in this study.
Bo
Tao
T
Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada.
Pawliszyn
Janusz
J
D019075:Electrophoresis, Capillary; D007525:Isoelectric Focusing; D007782:Lactoglobulins / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*; D010718:Phosphatidylserines / Q000737:chemistry*; D010763:Phosphorylase b / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D014361:Trypsin Inhibitors / Q000737:chemistry; D014362:Trypsinogen / Q000737:chemistry
D007782:Lactoglobulins; D008081:Liposomes; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D011506:Proteins; D014361:Trypsin Inhibitors; D014362:Trypsinogen; D010763:Phosphorylase b