title: Use of recombinant gonadotropin-releasing hormone antigens for immunosterilization of beef heifers.
abstract: The objectives of this study were to evaluate the effects of immunization against recombinant GnRH fusion proteins and growth promotants on onset of puberty, feedlot performance, and carcass characteristics of beef heifers. Heifers were immunized against an ovalbumin fusion protein containing 7 GnRH peptides (oGnRH, n = 12), a thioredoxin fusion protein containing 7 GnRH peptides (tGnRH, n = 12), a combination of oGnRH plus tGnRH (otGnRH, n = 12), or a combination of ovalbumin and thioredoxin (control, n = 11). Each heifer received a primary immunization containing 1 mg of protein in 1 mL of adjuvant injected into the mammary gland at wk 0 (mean age = 38 wk) and booster immunizations at wk 6 and 12. Six heifers within each treatment received Synovex H implants at wk -2. Weekly blood samples were collected from wk -2 to 26 for determination of serum progesterone concentrations and GnRH antibody titers. In GnRH-immunized heifers, GnRH antibody titers increased after the first booster injection, peaked after the second booster injection, and remained elevated through the end of the study (P < 0.01). Heifers immunized against oGnRH achieved greater (P < 0.05) GnRH antibody titers than tGnRH heifers but did not differ (P = 0.20) from otGnRH heifers. During the 26-wk study, ovulation was prevented (P < 0.05) in 10 out of 12, 12 out of 12, 11 out of 12, and 0 out of 11 tGnRH, oGnRH, otGnRH, and control heifers, respectively. At slaughter, uterine weights were lighter (P < 0.01) for GnRH-immunized heifers than control heifers. Synovex H-implanted heifers had greater (P < 0.05) ADG from wk -2 to 26, greater LM area, and lesser percentages of KPH, yield grade, and quality grade than nonimplanted heifers, regardless of the immunization treatment. Immunization against GnRH fusion proteins resulted in production of antibodies against GnRH that prevented ovulation in 92% of the heifers without affecting feedlot or carcass performance. Implanting heifers with Synovex H improved ADG, LM area, and yield grade. Improvements in delivery of the oGnRH vaccine may provide a feasible alternative to surgical spaying of heifers.
authors.affiliation: USDA-ARS, Fort Keogh Livestock and Range Research Laboratory, Miles City, MT 59301, USA. tom@larrl.ars.usda.gov
authors.forename: T W
authors.initials: TW
authors.lastname: Geary
authors.forename: E E
authors.initials: EE
authors.lastname: Grings
authors.forename: M D
authors.initials: MD
authors.lastname: MacNeil
authors.forename: D M
authors.initials: DM
authors.lastname: de Avila
authors.forename: J J
authors.initials: JJ
authors.lastname: Reeves
mesh_terms: D000818:Animals; D000858:Anovulation / Q000276:immunology; D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology*; D002417:Cattle / Q000502:physiology*; D004338:Drug Combinations; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology; D030762:Estrous Cycle / Q000276:immunology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology; D010053:Ovary / Q000187:drug effects; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013245:Sterilization, Reproductive / Q000379:methods / Q000662:veterinary*; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology; D013879:Thioredoxins / Q000276:immunology; D013997:Time Factors; D014599:Uterus / Q000187:drug effects; D022642:Vaccines, Contraceptive / Q000276:immunology*
chemical_list: D000906:Antibodies; D000941:Antigens; D004338:Drug Combinations; D011993:Recombinant Fusion Proteins; D022642:Vaccines, Contraceptive; C046521:estradiol benzoate, testosterone propionate drug combination; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D004958:Estradiol; D013879:Thioredoxins; D010047:Ovalbumin