[Arnebia root oil promotes wound healing and expression of basic fibroblast growth factor on the wound surface in rabbits].
OBJECTIVE
To observe the promoting effect of arnebia root oils on expression of basic fibroblast growth factor (bFGF) in skin wound of rabbits and the histomorphological changes in the wound surface, and to discuss its mechanism.


METHODS
Bilateral round skin wounds were made on the back of 15 rabbits. The three wounds on one side of the back of each rabbit were treated with arnebia root oils, while the three wounds on the other side were treated with vaseline in order to promote the wound healing. The histomorphology and ultrastructure under electron microscopy of the wounds, and the rate of wound healing were examined at different time. Western blotting assay was used to detect the expression of bFGF in the wound surface.


RESULTS
The healing rate of the arnebia root oils-treated wounds was evidently higher than that of the vaseline-treated wounds (P<0.05). The quantities of fibroblast, collagen and capillary in the arnebia root oils-treated wounds were much more than those in the vaseline-treated wounds, and the expression of endogenous bFGF in the arnebia root oils-treated wounds was enhanced obviously as compared with that in the vaseline-treated wounds in different period of wound healing. There existed a parallel correlation between the expression level of bFGF and the rate of wound healing.


CONCLUSIONS
The promoting effect of arnebia root oils on wound healing may be related to increasing the expression level of basic fibroblast growth factor in the skin wound.
Pei
Xian-Wu
XW
Department of Orthopedics, Second Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710004, China. peixianwu@126.com
Wang
Kun-Zheng
KZ
Dang
Xiao-Qian
XQ
Song
Jin-Hui
JH
Shi
Zhi-Bin
ZB
Gao
Deng-Feng
DF
D000818:Animals; D028082:Boraginaceae / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009821:Oils; D008517:Phytotherapy; D018517:Plant Roots / Q000737:chemistry; D011817:Rabbits; D012867:Skin / Q000293:injuries / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D009821:Oils; D016222:Fibroblast Growth Factor 2