title: Studies on the bile acids of the gall bladder bile of a mud-puppy, Salamander necturus.
abstract: The major bile acids of gall bladder bile of salamander necturus have been reported and shown to be taurine conjugates. The bile acid conjugates were hydrolysed and the free bile acids were separated by thin layer chromatography and identified through gas liquid chromatography and mass spectrometry (GC-MS) as methyl esters and methyl-ester trimethyl silyl ethers. About 56.3% of the total bus acid was present as allocholic acid whereas 3alpha, 7alpha, l2alpha-dihydroxy-5alpha-cholestanoic acid and cholic acid represented 30.4 and 12.3% respectively. Dihydroxy bile acids such as deoxycholic, allodeoxycholic and allochenodeoxycholic were present in less than 0.5% of the total bile acids.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Karachi, Karachi-75270, Pakistan.
authors.forename: S S
authors.initials: SS
authors.lastname: Ali