title: Polyenylphosphatidylcholine pretreatment protects rat liver from ischemia/reperfusion injury.
abstract: BACKGROUND
  Hepatic injury induced by ischemia/reperfusion following surgery, transplantation, or circulatory shock combined with resuscitation is a major clinical problem. Polyenylphosphatidylcholine (PPC) has strong antioxidant, cytoprotective and anti-inflammatory effects.
  
  
  OBJECTIVE
  In this study, the influence of PPC pretreatment on ischemia-reperfusion (I/R) injury of the liver was examined in rats.
  
  
  METHODS
  The animals were divided into three groups: control (n=10), I/R (n=15) and I/R+PPC (n=15). PPC was given 100mg/day for 7 days before experiment. Several parameters of hepatic damage, oxidative stress, neutrophil infiltration and nuclear factor kappa beta (NF-kappaB) expression were measured as well as microscopic examination.
  
  
  RESULTS
  We observed that a significant reduction in AST and ALT values in the PPC treated group when compared with the ischemic group. The increases in hepatic total NO(2)+NO(3) and MDA, and decreases in SOD and GSH levels after reperfusion were partially, but significantly, inhibited by PPC pretreatment. I/R induced increase in hepatic myeloperoxidase content and NF-kappaB expression were also lowered by PPC pretreatment. Animals pretreated with PPC presented minimal hemorrhage and reduced signs of liver injury.
  
  
  CONCLUSIONS
  PPC pretretament provided significant protection againts I/R injury to the liver. This treatment could be therapeutic in liver transplantation and other conditions associated with I/R injury.
authors.affiliation: Department of Pediatric Surgery, Medical School of Inonu University, Turkey.
authors.forename: Savaş
authors.initials: S
authors.lastname: Demirbilek
authors.forename: Abdurrahman
authors.initials: A
authors.lastname: Karaman
authors.forename: Kubilay
authors.initials: K
authors.lastname: Gürünlüoğlu
authors.forename: Erkan
authors.initials: E
authors.lastname: Taş
authors.forename: Melih
authors.initials: M
authors.lastname: Akın
authors.forename: Rauf Tuğrul
authors.initials: RT
authors.lastname: Aksoy
authors.forename: Emine
authors.initials: E
authors.lastname: Türkmen
authors.forename: Mehmet Naci
authors.initials: MN
authors.lastname: Edalı
authors.forename: Aysun
authors.initials: A
authors.lastname: Baykarabulut