Butea monosperma and chemomodulation: protective role against thioacetamide-mediated hepatic alterations in Wistar rats.
The present study was carried out to study the effect of Butea monosperma, a known liver acting drug on the tumor promotion related events of carcinogenesis in rat liver. Thioacetamide (TAA) was used to induce tumor promotion response and oxidative stress and caused significant depletion in the detoxification and antioxidant enzyme armory with concomitant elevation in malondialdehyde (MDA) formation, hydrogen peroxide (H(2)O(2)) generation, ornithine decarboxylase (ODC) activity and unscheduled DNA synthesis. However, B. monosperma pretreatment at two different doses restored the levels of the above-said parameters (p < 0.001) in a dose-dependent manner. The alcoholic extract of B. monosperma used in the present study seems to offer dose-dependent protection and maintain the structural integrity of hepatic cells. This was evident from the significant reduction in TAA-induced serum GOT, GPT, Lactate dehydrogenase (LDH) and gamma-Glutamyl transpeptidase activity (GGT) activities (p < 0.001). These investigations validate the use of B. monosperma in liver disorders by Ayurvedic physicians. Overall results indicate that the methanolic extract of B. monosperma possesses hepatoprotective effects and also it might suppress the promotion stage via inhibition of oxidative stress and polyamine biosynthetic pathway.
Sehrawat
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Section of Chemoprevention and Nutrition Toxicology, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), New Delhi, India.
Khan
T H
TH
Prasad
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Sultana
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S
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D031290:Butea / Q000737:chemistry*; D002273:Carcinogens / Q000378:metabolism; D002599:Chalcone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D047188:Chalcones; D056486:Chemical and Drug Induced Liver Injury; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008107:Liver Diseases / Q000188:drug therapy; D008297:Male; D008515:Medicine, Ayurvedic; D009955:Ornithine Decarboxylase / Q000097:blood / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013853:Thioacetamide / Q000633:toxicity; D000637:Transaminases / Q000097:blood / Q000187:drug effects; D005723:gamma-Glutamyltransferase / Q000097:blood / Q000187:drug effects
D015415:Biomarkers; D002273:Carcinogens; D047188:Chalcones; D005419:Flavonoids; D010936:Plant Extracts; D013853:Thioacetamide; C049386:butrin; C049385:isobutrin; D002599:Chalcone; D004247:DNA; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D000637:Transaminases; D009955:Ornithine Decarboxylase; D005978:Glutathione