title: Preliminary study of tissue-engineered odontogenesis in the canine jaw.
abstract: OBJECTIVE
  We previously reported a method for the development of tissue-engineered tooth. However, 1 drawback of the procedure was the inability to determine whether the tooth would function when transplanted in the jaw because it was formed in the omentum of the abdomen. Therefore, the present study was designed to evaluate whether transplantation of dissociated odontogenic cells could induce tissue-engineered odontogenesis in the canine jaw.
  
  
  METHODS
  Cells were harvested from canine first molar tooth buds and the resulting heterogeneous cell population was seeded on a biodegradable polymer. These constructs were then transplanted into the same sockets after extracting the tooth buds. After transplantation, we evaluated the transplanted constructs using dental x-ray, micro-computed tomography, histology, and immunohistochemistry.
  
  
  RESULTS
  After 24 weeks, micro-x-ray computed tomography showed regenerated hard tissues in the jaw, and hematoxylin and eosin staining showed tubular dentin and bone. In the regenerated tissue, osteopontin, osteonectin, and osteocalcin antibodies stained the dentinal matrix. However, enamel tissue and dental-root formation were not observed.
  
  
  CONCLUSIONS
  These data show for the first time the formation of dentin and bone from dissociated odontogenic cells in the canine jaw.
authors.affiliation: Tooth Regeneration, Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. honda-m@ims.u-tokyo.ac.jp
authors.forename: Masaki J
authors.initials: MJ
authors.lastname: Honda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ohara
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sumita
authors.forename: Takunori
authors.initials: T
authors.lastname: Ogaeri
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kagami
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueda
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D017690:Cell Transplantation / Q000379:methods*; D003804:Dentin / Q000254:growth & development; D004285:Dogs; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000601:surgery; D008962:Models, Theoretical; D008963:Molar / Q000000981:diagnostic imaging / Q000601:surgery; D009805:Odontogenesis; D011859:Radiography; D023822:Tissue Engineering / Q000379:methods*; D014083:Tooth Germ / Q000637:transplantation*; D014092:Tooth Root / Q000254:growth & development