Identification, cloning, and expression of the Scytalidium acidophilum XYL1 gene encoding for an acidophilic xylanase.
We cloned XYL1, a Scytalidium acidophilum gene encoding for an acidophilic family 11 xylanase. The XYL1p protein was expressed in Pichia pastoris using the pPICZalphaA expression plasmid. The secreted protein was purified by TAXI affinity column chromatography. The purified XYL1p showed an optimum activity at pH 3.2 and 56 degrees C. The Michaelis-Menten constants were determined.
Al Balaa
Bassam
B
Research Unit in Molecular Biology, University of Namur, Namur, Belgium.
Wouters
Johan
J
Dogne
Sophie
S
Rossini
Carlos
C
Schaus
Jean-Michel
JM
Depiereux
Eric
E
Vandenhaute
Jean
J
Housen
Isabelle
I
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data
D005656:Fungal Proteins