title: The pH-dependent unfolding mechanism of P2 myelin protein: an experimental and computational study.
abstract: The P2 protein is a small, extrinsic protein of the myelin membrane in the peripheral nervous system that structurally belongs to the fatty acid binding proteins (FABPs) family, sharing with them a 10 strands beta-barrel structure. FABPs appear to be involved in cellular fatty acid transport, but very little is known about the role of P2 in the metabolism of peripheral myelin lipids. Study of protein conformation at different pHs is a useful tool for the characterization of the unfolding mechanisms and the intrinsic conformational properties of the protein, and may give insight into factors that guide protein folding pathways. In particular, low pH conditions have been shown to induce partially folded states in several proteins. In this paper, the acidic unfolding of purified P2 protein was studied with both spectroscopic techniques and molecular dynamics simulation. Both experimental and computational results indicate the presence of a partly folded state at low pH, which shows structural changes mainly involving the lid that is formed by the helix-turn-helix domain. The opening of the lid, together with a barrel relaxation, could regulate the ligand exchanges near the cell membrane, supporting the hypothesis that the P2 protein may transport fatty acids between Schwann cells and peripheral myelin.
authors.affiliation: Dipartimento di Fisica, CNR-INFM and CNISM, Università di Parma, Parco Area delle Scienze, 7/A, 43100 Parma, Italy. eugenia.polverini@fis.unipr.it
authors.forename: Eugenia
authors.initials: E
authors.lastname: Polverini
authors.forename: Micaela
authors.initials: M
authors.lastname: Fornabaio
authors.forename: Anna
authors.initials: A
authors.lastname: Fasano
authors.forename: Giulia
authors.initials: G
authors.lastname: Carlone
authors.forename: Paolo
authors.initials: P
authors.lastname: Riccio
authors.forename: Paolo
authors.initials: P
authors.lastname: Cavatorta
mesh_terms: D000818:Animals; D019295:Computational Biology; D006863:Hydrogen-Ion Concentration; D018996:Myelin P2 Protein / Q000737:chemistry*; D011487:Protein Conformation; D017510:Protein Folding
chemical_list: D018996:Myelin P2 Protein