Real-time sizing of nanoparticles in microfluidic channels using confocal correlation spectroscopy.
This Communication reports real-time sizing of nanoparticles in microfluidic systems using confocal correlation spectroscopy (CCS). CCS can be used to measure the size of both fluorescent and nonfluorescent particles at low concentrations (<nanomolar) and in small volumes (<femtoliters). With advances in nonfluorescent modes of single-particle and single-molecule detection, we anticipate CCS will find broad use in the real-time measurements of nanoparticles and macromolecules.
Kuyper
Christopher L
CL
Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA.
Budzinski
Kristi L
KL
Lorenz
Robert M
RM
Chiu
Daniel T
DT
D005456:Fluorescent Dyes / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes