Postsynaptic TrkB signaling has distinct roles in spine maintenance in adult visual cortex and hippocampus.
In adult primary visual cortex (V1), dendritic spines are more persistent than during development. Brain-derived neurotrophic factor (BDNF) increases synaptic strength, and its levels rise during cortical development. We therefore asked whether postsynaptic BDNF signaling through its receptor TrkB regulates spine persistence in adult V1. This question has been difficult to address because most methods used to alter TrkB signaling in vivo affect cortical development or cannot distinguish between pre- and postsynaptic mechanisms. We circumvented these problems by employing transgenic mice expressing a dominant negative TrkB-EGFP fusion protein in sparse pyramidal neurons of the adult neocortex and hippocampus, producing a Golgi-staining-like pattern. In adult V1, expression of dominant negative TrkB-EGFP resulted in reduced mushroom spine maintenance and synaptic efficacy, accompanied by an increase in long and thin spines and filopodia. In contrast, mushroom spine maintenance was unaffected in CA1, indicating that TrkB plays fundamentally different roles in structural plasticity in these brain areas.
Chakravarthy
Sridhara
S
Department of Molecular Visual Plasticity, Netherlands Ophthalmic Research Institute, Royal Netherlands Academy of Arts and Sciences, Meibergdreef 47, 1105 BA Amsterdam, The Netherlands.
Saiepour
M Hadi
MH
Bence
Matthew
M
Perry
Sean
S
Hartman
Robin
R
Couey
Jonathan J
JJ
Mansvelder
Huibert D
HD
Levelt
Christiaan N
CN
D000818:Animals; D001921:Brain / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002468:Cell Physiological Phenomena; D004247:DNA / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology; D049229:Dendritic Spines; D004594:Electrophysiology; D005799:Genes, Dominant; D006056:Golgi Apparatus / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009473:Neuronal Plasticity; D009474:Neurons / Q000378:metabolism; D020813:Receptor, trkB / Q000378:metabolism*; D011995:Recombination, Genetic; D015398:Signal Transduction; D013569:Synapses / Q000378:metabolism; D009435:Synaptic Transmission; D013997:Time Factors; D014793:Visual Cortex / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D049452:Green Fluorescent Proteins; D004247:DNA; D020813:Receptor, trkB