Identification of the mstE gene encoding a glucose-inducible, low affinity glucose transporter in Aspergillus nidulans.
The mstE gene encoding a low affinity glucose transporter active during the germination of Aspergillus nidulans conidia on glucose medium has been identified. mstE expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor CreA. The expression of MstE and its subcellular distribution have been studied using a MstE-sGFP fusion protein. Concordant with data on mstE expression, MstE-sGFP is synthesized in the presence of repressing carbon sources, and fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane. Deletion of mstE has no morphological phenotype but results in the absence of low affinity glucose uptake kinetics, the latter being substituted by a high affinity system.
Forment
Josep V
JV
Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Apartado de Correos 73, Burjassot, 46100 Valencia, Spain.
Flipphi
Michel
M
Ramón
Daniel
D
Ventura
Luisa
L
Maccabe
Andrew P
AP
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics*; D015152:Blotting, Northern; D002462:Cell Membrane / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D016208:Databases, Factual; D020224:Expressed Sequence Tags; D005656:Fungal Proteins; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005947:Glucose / Q000493:pharmacokinetics / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D025301:Hyphae / Q000378:metabolism; D007700:Kinetics; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D013172:Spores, Fungal / Q000378:metabolism / Q000502:physiology; D013379:Substrate Specificity
D018076:DNA, Complementary; D005656:Fungal Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D049452:Green Fluorescent Proteins; D005947:Glucose