title: Some observations on the adaptation of Eimeria tenella (local isolates) sporozoites on chicken embryos through chorioallantoic membrane.
abstract: Eimeria (E.) tenella (local isolate) sporozoites were adapted on the chorioallantoic membrane (CAM) of 10-12 days chicken embryos and completed its life cycle in 6-7 days at 39 degrees C and 70 per cent humidity. Only 23 embryos (4.6%) were found dead from 1-4 day post inoculation of sporozoites with mild lesions on CAM with no gametocytes but few sporozoites in chorioallantoic fluid (CAF). On 5-7 day post inoculation, 432 embryos (86.4%) were found dead with severe haemorrhages on CAM and CAF contained uncountable number of gametocytes. After seven days post inoculation, 45 embryos (9%) were found to be alive. Some oocysts were also detected in the CAF on 6-7 days post inoculation. In the histological sections of the CAM, there were abundant small dark colored rounded bodies of gametes; distributed extensively in tissues of CAM on 5-7 days post inoculation of sporozoites. In some cases, cluster of small mature and immature relatively large bodies were seen in increasing numbers on 5-6 days post inoculation.
authors.affiliation: Immunoparasitology Laboratory, Department of Veterinary Parasitology, University of Agriculture, Faisalabad-38040, Pakistan.
authors.forename: M Abdul
authors.initials: MA
authors.lastname: Hafeez
authors.forename: Masood
authors.initials: M
authors.lastname: Akhtar
authors.forename: M Mazhar
authors.initials: MM
authors.lastname: Ayaz
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D016786:Eimeria tenella / Q000254:growth & development*; D006651:Histocytochemistry; D011201:Poultry Diseases / Q000469:parasitology*