Mycobacterium avium restriction fragment length polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains.
Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 AIDS inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns except for one band.
Sequeira
Patrícia Carvalho de
PC
Departamento de Micobacterioses, Instituto Oswaldo Cruz-Fiocruz, 21045-900 Rio de Janeiro, RJ, Brazil.
Fonseca
Leila de Souza
Lde S
Silva
Marlei Gomes da
MG
Saad
Maria Helena Féres
MH
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D001938:Brazil; D002645:Chickens / Q000382:microbiology; D005516:Food Microbiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D009162:Mycobacterium avium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000382:microbiology; D014675:Vegetables / Q000382:microbiology