title: Production and processing of milk from transgenic goats expressing human lysozyme in the mammary gland.
abstract: The potential for applying biotechnology to benefit animal agriculture and food production has long been speculated. The addition of human milk components with intrinsic antimicrobial activity and positive charge to livestock milk by genetic engineering has the potential to benefit animal health, as well as food safety and production. We generated one line of transgenic goats as a model for the dairy cow designed to express human lysozyme in the mammary gland. Here we report the characterization of the milk from 5 transgenic females of this line expressing human lysozyme in their milk at 270 microg/mL or 68% of the level found in human milk. Milk from transgenic animals had a lower somatic cell count, but the overall component composition of the milk and milk production were not different from controls. Milk from transgenic animals had a shorter rennet clotting time and increased curd strength. Milk of such nature may be of benefit to the producer by influencing udder health and milk processing.
authors.affiliation: Department of Animal Science, University of California, Davis 95616, USA. eamaga@ucdavis.edu
authors.forename: E A
authors.initials: EA
authors.lastname: Maga
authors.forename: C F
authors.initials: CF
authors.lastname: Shoemaker
authors.forename: J D
authors.initials: JD
authors.lastname: Rowe
authors.forename: R H
authors.initials: RH
authors.lastname: Bondurant
authors.forename: G B
authors.initials: GB
authors.lastname: Anderson
authors.forename: J D
authors.initials: JD
authors.lastname: Murray
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics* / Q000502:physiology; D000890:Anti-Infective Agents; D002452:Cell Count; D012085:Chymosin / Q000378:metabolism; D005260:Female; D005511:Food Handling / Q000379:methods; D015870:Gene Expression; D006041:Goats / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007774:Lactation; D008321:Mammary Glands, Animal / Q000201:enzymology*; D008892:Milk / Q000737:chemistry* / Q000166:cytology; D009113:Muramidase / Q000235:genetics*
chemical_list: D000890:Anti-Infective Agents; C025350:rennet; D009113:Muramidase; D012085:Chymosin