The effect of dietary supplementation with blueberry, alpha-tocopherol or astaxanthin on oxidative stability of Arctic char (Salvelinus alpinus) semen.
The objective was to determine the oxidative stability of Arctic char (Salvelinus alpinus) semen following dietary supplementation with lowbush blueberry (Vaccinium angustifolium) product, alpha-tocopherol, alpha-tocopherol+blueberry product, or alpha-tocopherol+astaxanthin. Sperm lipid peroxidation was initiated by challenging with ferrous sulphate/ascorbic acid (Fe(++)/Asc) at level of 0.04/0.2 mmol/L. Addition of blueberry, alpha-tocopherol, or both to char diets inhibited semen lipid peroxidation by: (a) decreasing the rate of sperm lipid peroxidation, an effect which was more pronounced with alpha-tocopherol treatments; and (b) increasing the antioxidant potential of seminal plasma, based on the lipid peroxidation process of sperm and an in vitro chicken brain tissue model. Dietary supplementation with astaxanthin and alpha-tocopherol had the same effect as the supplementation with alpha-tocopherol alone on inhibiting the lipid peroxidation process of sperm and chicken brain. Catalase-like activity increased significantly in sperm of fish fed alpha-tocopherol, blueberry, or both. There was a negative correlation (r= -0.397, P < 0.05) between catalase-like activity in sperm cells and the rate of sperm lipid peroxidation. Seminal plasma alpha-tocopherol levels increased significantly in fish supplemented with alpha-tocopherol alone or in combination with blueberry or astaxanthin. There were negative correlations between seminal plasma alpha-tocopherol levels and lipid peroxidation rates of sperm cells (r= -0.625, P < 0.01) and brain tissue (r= -0.606, P < 0.01). In conclusion, dietary supplementation of blueberry product or alpha-tocopherol inhibited lipid peroxidation in Arctic char semen. Further experiments are needed to test the effect of dietary blueberry and antioxidants on Arctic char semen quality during liquid and cryopreserved storage.
Mansour
Nabil
N
Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PEI, C1A 4P3, Canada. nabilman99@yahoo.com
McNiven
Mary A
MA
Richardson
Gavin F
GF
D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002645:Chickens; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D005638:Fruit / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D010084:Oxidation-Reduction; D012661:Semen / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D014337:Trout / Q000502:physiology*; D024341:Xanthophylls / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000494:pharmacology
D000975:Antioxidants; D004338:Drug Combinations; D024341:Xanthophylls; C005948:astaxanthine; D002374:Catalase; D024502:alpha-Tocopherol