title: [Effect of phenolic alkaloids of Menispermum dauricum on thrombosis and platelet aggregation].
abstract: OBJECTIVE
  To observe the effect of phenolic alkaloids of Menispermum dauricum (PAMD) on thrombosis and platelet aggregation, and to explore its mechanism of action.
  
  
  METHODS
  Thrombosis was observed with arteriovenous shunt thrombus model in rat; platelet aggregation was determined by Born's method; ultrastructure of platelet was observed by transmission electron microscope; TXB2 or 6-keto-PGF1alpha levels were assessed by radioimmunoassay; and NO was determined by colorimetric method.
  
  
  RESULTS
  PAMD dose-dependently inhibited experimental thrombus formation, platelet aggregation induced by ADP, AA and THR in vivo and ultrastructure changes stimulated by THR; PAMD increased the generation of 6-keto-PGF1alpha in thoracic aortae and NO level in plasma; and had no influence on TXB2 release (P > 0.05).
  
  
  CONCLUSIONS
  PAMD inhibited thrombosis and platelet aggregation, and its mechanism might be due to the increase of PGI2 and NO level.
authors.affiliation: Department of Pharmacology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. kong0051@hotmail.com
authors.forename: Xiang-Ying
authors.initials: XY
authors.lastname: Kong
authors.forename: Pei-Li
authors.initials: PL
authors.lastname: Gong
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000470:Alkaloids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D044182:Benzylisoquinolines / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D001792:Blood Platelets / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D011464:Epoprostenol / Q000378:metabolism; D008297:Male; D031603:Menispermum / Q000737:chemistry; D009569:Nitric Oxide / Q000097:blood; D010946:Plants, Medicinal / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry; D044005:Tetrahydroisoquinolines / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D013927:Thrombosis / Q000378:metabolism*; D013929:Thromboxane B2 / Q000378:metabolism
chemical_list: D000470:Alkaloids; D044182:Benzylisoquinolines; D044005:Tetrahydroisoquinolines; D009569:Nitric Oxide; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C023968:daurisoline; C035934:dauricine; D011464:Epoprostenol