YHV-protease dsRNA inhibits YHV replication in Penaeus monodon and prevents mortality.
Yellow head virus infects cultured shrimps and causes severe mortality resulting in a great economic loss. Haemolymph injection of dsRNA(pro) corresponding to the protease motif of YHV genome resulted in a complete inhibition of YHV replication. The effect of dsRNA lasted for at least 5 days. Injecting sequence-unrelated dsRNA(gfp) or dsRNA(TSV-pol) also resulted in an inhibition of YHV replication but at a comparatively much less extent. Shrimp mortality was monitored for 10 days when more than 90% shrimps receiving no dsRNA died within 8 dpi. However, those receiving dsRNA(pro) showed no mortality. A partial mortality was observed among the shrimps receiving dsRNA(gfp) or dsRNA(TSV-pol). Thus, Penaeus monodon possesses the sequence-specific protection to YHV infection, most likely through the RNAi pathway, in addition to sequence-independent protection. It gives a new notion that dsRNA induction of antiviral immunity in shrimp goes through two pathways, sequence-independent and sequence-dependent.
Yodmuang
Supansa
S
The Institute of Molecular Biology and Genetics, Mahidol University, Salaya, Nakornpathom 73170, Thailand.
Tirasophon
Witoon
W
Roshorm
Yaowaluck
Y
Chinnirunvong
Wanlop
W
Panyim
Sakol
S
D000818:Animals; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D006458:Hemolymph / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics* / Q000821:virology; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046828:Roniviridae / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014779:Virus Replication
D012330:RNA, Double-Stranded; D011994:Recombinant Proteins