Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX prophage.
Vibrio cholerae O1 isolates belonging to the Ogawa serotype, El Tor biotype, harbouring the classical CTX prophage were first isolated in Mozambique in 2004. Multilocus sequence typing (MLST) analysis using nine genetic loci showed that the Mozambique isolates have the same sequence type (ST) as O1 El Tor N16961, a representative of the current seventh cholera pandemic. Analysis of the CTX prophage in the Mozambique isolates indicated that there is one type of rstR in these isolates: the classical CTX prophage. It was also found that the ctxB-rstR-rstA-rstB-phs-cep fragment was PCR-amplified from these isolates, which indicates the presence of a tandem repeat of the classical CTX prophage in the genome of the Mozambique isolates. The possible origin of these isolates and the presence of the tandem repeat of the classical prophage in them implicate the presence of the classical CTX phage.
Lee
Je Hee
JH
School of Biological Sciences, Seoul National University, Seoul, 151-742, Republic of Korea.|International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
Han
Kyung Ho
KH
International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
Choi
Seon Young
SY
School of Biological Sciences, Seoul National University, Seoul, 151-742, Republic of Korea.|International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
Lucas
Marcelino E S
MES
Ministry of Health, Maputo, Mozambique.
Mondlane
C
C
Centro De Higiene Ambiental E Exames Medicos, Rua Correia De Brito N° 1815 Sofala - Beira, Mozambique.
Ansaruzzaman
M
M
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
Nair
G Balakrish
GB
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
Sack
David A
DA
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
von Seidlein
Lorenz
L
International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
Clemens
John D
JD
International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
Song
Manki
M
International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
Chun
Jongsik
J
School of Biological Sciences, Seoul National University, Seoul, 151-742, Republic of Korea.|International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
The Mozambique Cholera Vaccine Demonstration Project Coordination Group
International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
Kim
Dong Wook
DW
International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
D001426:Bacterial Proteins / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics; D014644:Genetic Variation; D016680:Genome, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009073:Mozambique; D039002:Prophages / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020080:Tandem Repeat Sequences / Q000235:genetics; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000235:genetics
D001426:Bacterial Proteins; D012097:Repressor Proteins; C107871:RstR protein, Vibrio cholerae