Vital growth factors of Malassezia species on modified CHROMagar Candida.
A comparison of several media, i.e., potato dextrose agar with olive oil (Oil-PDA), modified Dixon agar (mDIX) and variations of Leeming and Notman agar (LNA) for the isolation and growth of Malassezia and Candida species was examined. Since LNA supported the highest growth of Malassezia species its key components, i.e., ox bile, glycerol monostearate, glycerol and Tween 60, were added to CHROMagar Candida. All 7 species of Malassezia grew well on this modified medium (LN-CHROM) after incubation for 4 days at 30 degrees C and development was equal to that observed on LNA. Colonies on LN-CHROM were smooth and from pink to dark purple in color. Furthermore, the use of LN-CHROM did not alter the colony characteristics of Candida species as compared to that found on CHROMagar Candida. The results of the present investigation indicate that the use of LN-CHROM would make possible the simultaneous isolation and identification of Malassezia and Candida species.
Kaneko
Takamasa
T
Kanto Chemical Co., Inc. Marusan BLDG. 11-5, Nihonbashi Honcho, 3-Chome, Chuo-ku, Tokyo 103-0023, Japan.
Makimura
Koichi
K
Onozaki
Masanobu
M
Ueda
Kumiko
K
Yamada
Yohko
Y
Nishiyama
Yayoi
Y
Yamaguchi
Hideyo
H
D001646:Bile / Q000502:physiology; D002175:Candida / Q000254:growth & development*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D005989:Glycerides / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D008290:Malassezia / Q000254:growth & development*; D009172:Mycology / Q000379:methods; D011136:Polysorbates / Q000494:pharmacology
D003470:Culture Media; D005989:Glycerides; D006133:Growth Substances; D011136:Polysorbates; C048159:glyceryl monostearate; D005990:Glycerol