title: Tolerance induction by third-party "off-the-shelf" CD4+CD25+ Treg cells.
abstract: OBJECTIVE
  Recent reports have shown that donor or host CD4(+)CD25(+) Treg cells can be used to control GVHD or graft rejection following allogeneic BMT in mice. In the present study we investigated the potential of third-party Treg cells compared to donor-type cells to facilitate BM allografting.
  
  
  METHODS
  Graft rejection is assessed in a mouse model of T cell-mediated BM allograft rejection. Lethally irradiated C3H mice are transplanted at day 2 after irradiation with T cell-depleted Balb/Nude BM. Graft rejection is induced by purified host-type T cells infused one day prior to BMT. Cells tested for their facilitating activity are added to the T cell-depleted BM allograft.
  
  
  RESULTS
  Naïve or ex vivo-expanded third-party Treg cells can effectively enhance engraftment of T cell-depleted BM allografts, exhibiting reactivity in vitro and in vivo similar to that found for donor-type Treg cells.
  
  
  CONCLUSIONS
  The use of third-party Treg cells in contrast to donor-type cells could allow advanced preparation of a large bank of Treg cells (off-the-shelf), with all the appropriate quality controls required for cell therapy.
authors.affiliation: Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: David
authors.initials: D
authors.lastname: Steiner
authors.forename: Noga
authors.initials: N
authors.lastname: Brunicki
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Blazar
authors.forename: Esther
authors.initials: E
authors.lastname: Bachar-Lustig
authors.forename: Yair
authors.initials: Y
authors.lastname: Reisner
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D006084:Graft Rejection / Q000276:immunology; D006086:Graft vs Host Disease / Q000276:immunology; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D020123:Sirolimus / Q000008:administration & dosage; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D018183:Transplantation Chimera; D014181:Transplantation Immunology / Q000276:immunology; D023001:Transplantation Tolerance / Q000187:drug effects / Q000528:radiation effects; D014184:Transplantation, Homologous / Q000009:adverse effects
chemical_list: D020123:Sirolimus