title: Separation and determination of enkephalin-related peptides using capillary electrophoresis.
abstract: CZE with UV-absorption detection has been used for the separation and determination of enkephalin-related peptides. The experimental conditions, such as pH and concentration of running buffer, applied voltage, injection method, and time, were investigated in detail. Excellent separation efficiency could be obtained for ten enkephalin-related peptides with a 50 microm (ID) x 58 cm capillary using sodium dihydrogen phosphate as the running buffer (pH 3.11) when 20 kV of applied voltage was used. The concentration detection limits were found to be in the range of 0.31-1.94 microg/mL (defined as S/N = 3). The proposed method has been applied to analyze the spiked cerebrospinal fluid (CSF) sample, and the results showed that CZE is a powerful technique for separation and detection of the above biological peptides.
authors.affiliation: Department of Chemistry, Fuzhou University, Fuzhou, Fujian, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Jianping
authors.initials: J
authors.lastname: Duan
authors.forename: Xiuyan
authors.initials: X
authors.lastname: Jiang
authors.forename: Hongqing
authors.initials: H
authors.lastname: Chen
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D002555:Cerebrospinal Fluid / Q000378:metabolism; D002621:Chemistry / Q000379:methods*; D004586:Electrophoresis / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004745:Enkephalins / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D010756:Phosphoric Acids / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000032:analysis
chemical_list: D004745:Enkephalins; D010455:Peptides; D010756:Phosphoric Acids; D014867:Water; C030242:phosphoric acid