[Effect of interleukin-8 in cell invasion and proliferation of human breast cancer].
OBJECTIVE
To identify the effect of interleukin-8 in cell progression and invasion of human breast cancer.


METHODS
Human cytokine antibody arrays were applied to screen a panel of cytokine expression from 11 human breast cancer cell lines, and the mechanism of identified key factors involved in breast cancer progression was studied.


RESULTS
Profiling of cytokine expression showed the expression of interleukin-8 was related to estrogen receptor status, metastasis and vimentin status in the 11 human breast cancer cell lines. Elevated expression of interleukin-8 in breast cancer cells had positive correlation with breast cancer invasion. Neutralization of antibody against interleukin-8 specifically blocked interleukin-8-mediated cell invasion. However, anti-interleukin-8 antibody did not influence the proliferation of breast cancer cells.


CONCLUSIONS
Interleukin-8 may be the key factor involved in human breast cancer progression and invasion, and play an important role in cell invasion of breast cancer.
Lin
Ying
Y
Center of Breast Disease, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Wang
Shen-ming
SM
Lü
Wei-ming
WM
Huang
Ruo-pan
RP
D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000096:biosynthesis / Q000502:physiology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D040081:Protein Array Analysis; D011960:Receptors, Estrogen / Q000378:metabolism; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D016209:Interleukin-8; D011960:Receptors, Estrogen; D016159:Tumor Suppressor Protein p53