Development of a guinea pig immune response-related microarray and its use to define the host response following Mycobacterium bovis BCG vaccination.
Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive animals. Eleven genes were significantly (P < 0.05) up-regulated following vaccination, indicating a Th1-type response. These results show that microarrays can be used to more fully define immune profiles of guinea pigs.
Tree
Julia A
JA
Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury, Wiltshire SP4 0JG, United Kingdom. julia.tree@hpa.org.uk
Elmore
Michael J
MJ
Javed
Sajid
S
Williams
Ann
A
Marsh
Philip D
PD
D000818:Animals; D021461:Animals, Outbred Strains; D001500:BCG Vaccine / Q000008:administration & dosage* / Q000276:immunology*; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006168:Guinea Pigs; D008213:Lymphocyte Activation; D009163:Mycobacterium bovis / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D014373:Tuberculin / Q000008:administration & dosage / Q000276:immunology; D014376:Tuberculosis / Q000276:immunology* / Q000517:prevention & control; D014611:Vaccination
D001500:BCG Vaccine; D018925:Chemokines; D016207:Cytokines; D014373:Tuberculin