title: A modified HIV challenge assay in mice by using luciferase-expressing vaccinia virus.
abstract: In this study, we developed a simple and sensitive assay in mice for a challenge experiment by using a recombinant vaccinia virus dual-expressing antigen (HIV Env gp160) and firefly luciferase. This assay can detect the vaccine effect at real-time in vivo by using a small amount of mouse serum. The luciferase activity in mouse serum was in agreement with the viral titer in the ovary. This assay would be applicable as a challenge model for infectious diseases.
authors.affiliation: Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Shinoda
authors.forename: Ke-Qin
authors.initials: KQ
authors.lastname: Xin
authors.forename: Kenji
authors.initials: K
authors.lastname: Okuda
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D017930:Genes, Reporter; D006678:HIV / Q000235:genetics*; D019302:HIV Envelope Protein gp160 / Q000235:genetics* / Q000276:immunology; D015658:HIV Infections; D008156:Luciferases / Q000097:blood / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D010053:Ovary / Q000821:virology; D012680:Sensitivity and Specificity; D044967:Serum / Q000821:virology; D013194:Staining and Labeling / Q000379:methods; D014616:Vaccinia virus / Q000235:genetics* / Q000502:physiology
chemical_list: D016915:AIDS Vaccines; D019302:HIV Envelope Protein gp160; D008156:Luciferases