Rapid and sensitive liquid chromatography-tandem mass spectrometry method for the estimation of nicorandil in human plasma.
A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the estimation of nicorandil in human plasma. Nicorandil was extracted from human plasma using solid-phase extraction technique. Imipramine was used as the internal standard. A Betasil C18 column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves a rapid solid-phase extraction from plasma, simple isocratic chromatography conditions and mass spectrometric detection that enables detection at nanogram levels. The proposed method has been validated for a linear range of 1.0-500.0 ng/mL with a correlation coefficient of > or =0.9993. The intra-run and inter-run precision and accuracy was within 10.0%. The overall recovery for nicorandil was 63.81%. The total run time was just 3.0 min.
Bhatt
Jignesh
J
Torrent Research Centre, Gandhinagar-382428, Gujarat, India. jigu_baruda@rediffmail.com
Jangid
Arvind
A
Shah
Bhavin
B
Shetty
Raghavendra
R
Kambli
Sandeep
S
Subbaiah
Gunta
G
Singh
Sadhana
S
D006801:Humans; D020108:Nicorandil / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D014665:Vasodilator Agents / Q000097:blood*
D014665:Vasodilator Agents; D020108:Nicorandil