title: Increased expression of Toll like receptor 4 on peripheral-blood mononuclear cells in patients with coronary arteriosclerosis disease.
abstract: The family of Toll-like receptors (TLRs) initiates innate immune responses, and Toll-like receptor 4 (TLR4) was considered to be an important player in the initiation and progression of atherosclerotic disease. The aim of the study was to investigate the expression of TLR4 on peripheral-blood mononuclear cells (PBMCs) in patients with coronary arteriosclerosis disease (CAD). We have examined the expression of TLR4 protein and mRNA by flow cytometry (FCM) and real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). In addition, the levels of plasma lipids were determined by automatic biochemistry analyzer. The results showed that the positive rates and the mean mRNA copy number of TLR4 in CAD group were significantly higher than that in controls. But no significant difference was found in the positive rate and the mean mRNA copy number of TLR4 between CAD group with normal level of plasma lipids and the CAD group with abnormal level of plasma lipids. We suggest that expression level of TLR4 on peripheral-blood mononuclear cells is increased in atherosclerotic, but the differential expression of TLR4 has no correlation with the level of plasma lipids.
authors.affiliation: Department of Laboratory Diagnosis, Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors.forename: H-L
authors.initials: HL
authors.lastname: Geng
authors.forename: H-Q
authors.initials: HQ
authors.lastname: Lu
authors.forename: L-Z
authors.initials: LZ
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: R-Q
authors.initials: RQ
authors.lastname: Zhong
mesh_terms: D000368:Aged; D002478:Cells, Cultured; D003324:Coronary Artery Disease / Q000097:blood / Q000276:immunology*; D018076:DNA, Complementary / Q000276:immunology; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008055:Lipids / Q000097:blood; D008297:Male; D010957:Plasmids / Q000276:immunology; D012333:RNA, Messenger / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D051197:Toll-Like Receptor 4 / Q000276:immunology*
chemical_list: D018076:DNA, Complementary; D008055:Lipids; D012333:RNA, Messenger; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4