title: Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms.
abstract: The genetic variability of 61 Trypanosoma cruzi isolates from 47 chronic chagasic patients of Minas Gerais state was analyzed by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) using M13-40, lambdagt11-F, and L15996 primers. Cluster analysis by unweighted pair group method analysis was applied to RAPD profiles, and cluster analysis used to verify a possible correlation among different clinical forms of the disease from these patients. The T. cruzi isolates showed distinct grouping on tree topology, with the isolates not being possible to establish a correlation to the clinical forms of Chagas' disease. These data showed that the T. cruzi isolates from these patients would compose a group of populations well correlated genetically.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 Caixa Postal 486, 31270-901, Belo Horizonte, Minas Gerais, Brazil. galvao@icb.ufmg.br
authors.forename: D A
authors.initials: DA
authors.lastname: D'Avila
authors.forename: E D
authors.initials: ED
authors.lastname: Gontijo
authors.forename: E
authors.initials: E
authors.lastname: Lages-Silva
authors.forename: W S F
authors.initials: WS
authors.lastname: Meira
authors.forename: E
authors.initials: E
authors.lastname: Chiari
authors.forename: L M C
authors.initials: LM
authors.lastname: Galvão
mesh_terms: D000328:Adult; D000818:Animals; D001938:Brazil; D014355:Chagas Disease / Q000469:parasitology* / Q000503:physiopathology*; D002648:Child; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D016054:DNA, Protozoan