Fabrication of antibody arrays using thermally responsive elastin fusion proteins.
A general method has been developed to immobilize antibodies onto an array surface by employing fusion proteins consisting of an elastin domain with tunable hydrophobic properties and an antibody-binding domain with high binding affinity and specificity for antibodies. Antibodies conjugated with the elastin fusion proteins can be directly printed on a self-assembled monolayer-modified glass slide in a functionally active orientation with a spatially defined pattern. An antibody array sensor for detection of tumor markers was fabricated to demonstrate the utility of the method. We expect that the method presented here could be a simple and universal platform to immobilize antibodies for the fabrication of a variety of antibody array sensors.
Gao
Di
D
Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, USA.
McBean
Nicole
N
Schultz
Jerome S
JS
Yan
Yushan
Y
Mulchandani
Ashok
A
Chen
Wilfred
W
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry; D004549:Elastin / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D009419:Nerve Tissue Proteins / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013205:Staphylococcal Protein A / Q000737:chemistry
D000906:Antibodies; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C029152:G-substrate; C116593:L-protein, Peptococcus magnus; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; D004549:Elastin