[Effect of GM-CSF on expansion and differentiation of CD34+ megakaryocyte progenitor cells from cord blood in vitro].
To study the effect of GM-CSF on in vitro expansion of megakaryocyte progenitor cells from cord blood, CD34(+) cells isolated by magnetic cell sorting system (MACS) were cultured in serum-free medium containing TPO, IL-3, SCF and with or without various concentrations of GM-CSF (5, 20, 100 ng/ml). The numbers of MNC, proportion of CD34(+)CD41(+) cells and CFU-MK were measured at 6, 10 and 14 days. The results showed that the expansion of MNC and proportion of CD41(+) cells was accelerated distinctly by various concentrations of GM-CSF after 14 days, while 20 and 100 ng/ml GM-CSF exhibited higher expansion effect than that of 5 ng/ml. TPO + IL-3 + SCF with 5 ng/ml or 20 ng/ml GM-CSF could stimulate the formation of CFU-MK, while TPO + IL-3 + SCF with 100 ng/ml GM-CSF could inhibit it. It is concluded that GM-CSF can accelerate the expansion of megakaryocyte progenitor cells from CD34(+) cells in cord blood in the serum-free medium containing TPO + IL-3 + SCF.
Chen
Shu
S
Institute of Blood Transfusion, Blood Center of Zhejiang Province, Hangzhou 310006, China.
Zhu
Fa-Ming
FM
He
Ji
J
Liu
Jin-Hui
JH
Qin
Fei
F
Yan
Li-Xing
LX
D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
D018952:Antigens, CD34; D016178:Granulocyte-Macrophage Colony-Stimulating Factor