title: An immunocytometric assay based on dengue infection via DC-SIGN permits rapid measurement of anti-dengue neutralizing antibodies.
abstract: Dengue remains a global public health threat and development of a safe and effective vaccine is a principal public health goal. The primary correlate of immunity is thought to be neutralizing antibodies. Currently, the plaque reduction neutralization test (PRNT) is the gold standard measure of dengue neutralizing antibody responses, but this test is limited by time-consuming performance. In addition, some feel that use of viral strains adapted to grow in Vero or BHK cells may not accurately reflect protective responses. A human cell line transfected to express a putative natural dengue receptor, DC-SIGN (CD209), was used to measure antibody-mediated dengue neutralization. Using neutralizing monoclonal antibodies, immune sera, and laboratory adapted dengue viruses, serotype-specific neutralizing activity was demonstrated similar to that seen in the Vero PRNT. Importantly, serotype-specific neutralizing activity against recently isolated dengue strains with less heterotypic cross-neutralization than laboratory adapted viruses was also demonstrated.
authors.affiliation: Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Martin
authors.forename: Jorge
authors.initials: J
authors.lastname: Pardo
authors.forename: Monika
authors.initials: M
authors.lastname: Simmons
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Tjaden
authors.forename: Susana
authors.initials: S
authors.lastname: Widjaja
authors.forename: Mary A
authors.initials: MA
authors.lastname: Marovich
authors.forename: Wellington
authors.initials: W
authors.lastname: Sun
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Porter
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Burgess
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D015815:Cell Adhesion Molecules / Q000378:metabolism; D045744:Cell Line, Tumor / Q000378:metabolism; D003715:Dengue / Q000097:blood*; D003716:Dengue Virus / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism; D051379:Mice; D009500:Neutralization Tests / Q000379:methods*; D011956:Receptors, Cell Surface / Q000378:metabolism; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin