Multiple antibiotic resistance among gram negative bacteria isolated from milk in Karachi.
A total of 100 Gram negative bacteria isolated from milk in Karachi were screened for their resistance to eleven commonly used antibiotics. The concentration of antibiotics used was 100 microg/ml. About 46% were found to resist antibiotics at the concentration of 100 microg/ml giving different patterns. The resistant bacteria were tested for the presence of R plasmids, both transferable and non-transferable, by conjugation, spontaneous segregation and curing with acridine orange and ethidium bromide. Among the resistant bacteria tested, eight were found to carry non-transferable R plasmids.
Ahmed
A
A
Department of Microbiology, University of Karachi, Karachi-75270, Pakistan.
Jameel
N
N
Ansari
F A
FA
Khatoon
H
H