The role of Ca2+ in (-)-menthol-induced human promyelocytic leukemia HL-60 cell death.
A human promyelocytic leukemia HL-60 cell line was selected to examine the effect of (-)-Menthol on cell death. Based on the results from morphological changes and the percentage of viable cells in HL-60 cells after treatment with various concentrations of (-)-Menthol, it was shown that (-)-Menthol induced cell death through necrosis, not apoptosis. No cell cycle arrest was found in HL-60 cells examined by flow cytometry analysis. Also, the DNA gel electrophoresis method showed that (-)-Menthol did not induce apoptosis in HL-60 cells. However, it was found that (-)-Menthol induced the production of Ca2+ in these examined cells, dose-dependently. When HL-60 cells were pretreated with the chelator (BAPTA) of Ca2+ for 3 hours before addition of (-)-Menthol to the culture, a decrease of Ca2+ production was observed. Under the same conditions, the percentage of viable HL-60 cells was increased. Apparently Ca2+ production is associated with the induction of (-)-Menthol-induced cell death.
Lu
Hsu-Feng
HF
Department of Clinical Pathology, Cheng Hsin Rehabilitation Medical Center, Taipei, Taiwan, ROC.
Hsueh
Shu-Ching
SC
Yu
Fu-Shun
FS
Yang
Jai-Sing
JS
Tang
Nou-Ying
NY
Chen
Ssu-Ching
SC
Chung
Jing-Gung
JG
D000107:Acetylation; D002118:Calcium / Q000502:physiology*; D016923:Cell Death / Q000187:drug effects* / Q000502:physiology*; D053938:DNA Fragmentation; D004533:Egtazic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives; D004586:Electrophoresis; D005449:Fluorenes / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D008610:Menthol / Q000494:pharmacology*
D005449:Fluorenes; D008610:Menthol; C012177:2-aminofluorene; D004533:Egtazic Acid; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium