Effects of Scutellariae Radix on gene expression in HEK 293 cells using cDNA microarray.
The aim of the present study was to elucidate the molecular mechanisms underlying the anti-inflammatory effect of Scutellaria Radix (SR). The complementary DNA (cDNA) microarray method was used to survey the effects of SR on the changes of gene expression profile in HEK293 cells. Based on differential expression, 66 genes were selected for further analysis from 9,600 candidate genes in the microarray; 23 genes were validated by RT-PCR. The broad spectrum of the differentially expressed genes, including those associated with inflammation, immune response, energy metabolism, as well as others, such as ISGF3G, IL6ST, CD98, ATP5G2, PHKG2, YB-1 and SLC7A4, indicate overall cellular response to SR treatment. Our results suggest that the anti-inflammatory effect of SR may be related to IL6ST down-expression, and over-expression of CD98. Moreover, SR-related improvement in immune response may be related to the ISGF3G over-expression.
Chen
Chia-Sheng
CS
Institute of Chinese Medical Sciences, China Medical University, 91 Hsueh Shih Road, Taichung 404, Taiwan, ROC.
Chen
Nae-Jing
NJ
Lin
Li-Wei
LW
Hsieh
Chia-Chang
CC
Chen
Guang-Wei
GW
Hsieh
Ming-Tsuen
MT
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D050822:Cytokine Receptor gp130 / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D050834:Interferon-Stimulated Gene Factor 3, gamma Subunit / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D031364:Scutellaria baicalensis
D000893:Anti-Inflammatory Agents; C494199:IL6ST protein, human; C494227:IRF9 protein, human; D050834:Interferon-Stimulated Gene Factor 3, gamma Subunit; D010936:Plant Extracts; D050822:Cytokine Receptor gp130