The effects of anti-P-selectin antibody on leucocyte activity related to cigarette smoke in rats.
Nicotine-induced expression of P-selectin is implicated in endothelial cell damage related to smoking. Thirty male Sprague-Dawley rats were divided into three groups: two experimental and one control. Both experimental groups were exposed to cigarette smoke for four weeks, but one group was also given anti-P-selectin antibody (100 microg IgG per 100 g body weight) intravenously. Rolling and adhesion leucocytes within the microcirculation of the cremaster muscle were measured. The urine cotinine concentrations of rats exposed to smoke were 612 ng/ml higher than those of non-smokers. Both rolling and adherent leucocytes were highest and steady in the group given anti-P-selectin antibody at about 50 and 10, respectively. There was a significant drop in both rolling and adherent leucocytes (31 to 4 and 5 to 2) after the anti-P-selectin antibody had been given. However, this effect was short-lived as both increased above those at 35 minutes by 120 minutes (p<0.001). The increase in leucocyte rolling and adherence caused by smoking can be lowered by giving anti-P-selectin antibody. The effective period in rats was two hours, with the maximal effect one hour after injection.
Chen
Samuel Huan-Tang
SH
Department of Plastic and Reconstructive Surgery, Chang Gung University and Memorial Hospital, College of Medicine, 199 Tung Hwa North Road, Taipei, Taiwan. ma117@adm.cgmh.org.tw
Chang
Ting-Chang
TC
Chen
Lily Meng-Ju
LM
Hou-Hsun Chen
Patrick
P
Huang
Chen-Yi
CY
Wei
Fu-Chan
FC
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D003367:Cotinine / Q000652:urine; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008833:Microcirculation / Q000276:immunology; D019007:P-Selectin / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014652:Vascular Diseases / Q000276:immunology
D000906:Antibodies; D019007:P-Selectin; D014028:Tobacco Smoke Pollution; D003367:Cotinine