Papillomavirus E1 protein binds to and stimulates human topoisomerase I.
The papillomavirus (PV) E1 helicase plays a direct role in recruiting cellular DNA replication factors, such as replication protein A or polymerase alpha-primase, to replicate PV genomes. Here, E1 is shown to bind to human topoisomerase I and stimulate its relaxation activity up to sevenfold. The interaction between E1 and topoisomerase I was mapped to the E1 DNA binding domain and C terminus. These findings imply a mechanism for the recruitment of topoisomerase I to PV DNA replication forks and for stimulating topoisomerase I to allow for efficient relaxation of the torsional stress induced by replication fork progression.
Clower
Randolph V
RV
Department of Microbiology & Immunology, The School of Medicine and Biomedical Sciences, University at Buffalo, 213 Biomedical Research Building, 3435 Main Street, Buffalo, NY 14214, USA.
Fisk
John C
JC
Melendy
Thomas
T
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D004261:DNA Replication; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D052140:Human papillomavirus 11 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C069814:E1 protein, Bovine papillomavirus; C084304:E1 protein, Human papillomavirus type 11; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D004264:DNA Topoisomerases, Type I