Allele distribution of five X-chromosome short tandem repeat loci in Ewenke population of north China.
OBJECTIVE
To study the allele genetic polymorphism of five short tandem repeat (STR) loci on X-chromosome in Ewenke population of north China and to provide basic data for forensic identification.


METHODS
Genomic DNA was extracted from EDTA-whole blood of Ewenke population by Chelex-100. The DNA samples were amplified by PCR and were analyzed by polyacrylamide gel electrophoresis and silver staining. The sequence length variations of DXS6799, DXS8378, DXS101, HPRTB, and DXS6789 loci on X-chromosome in 98 unrelated Ewenke individuals were investigated.


RESULTS
All five loci analyzed showed high polymorphism and genetic stability. The data of the five X-chromosome STR loci in Ewenke ethnic group of China was in accordance with Hardy-Weinberg equilibrium by Chi-square test.


CONCLUSIONS
Allele polymorphism of five X-chromosome STR loci can be used as a genetic marker for forensic identification and population genetic research.
Gu
Shan-zhi
SZ
Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi'an Jiaotong University, Xi'an 710061.
Chen
Teng
T
Liu
Qing-bo
QB
Yu
Bing
B
Li
Sheng-bin
SB
D044466:Asian People; D002681:China / Q000208:ethnology; D041321:Chromosomes, Human, X / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D005554:Forensic Medicine; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D005819:Genetic Markers; D004247:DNA