title: Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus.
abstract: Development of reliable and eco-friendly process for synthesis of metallic nanoparticles is an important step in the filed of application of nanotechnology. One of the options to achieve this objective is to use natural processes such as use of biological systems. In this work we have investigated extracellular biosynthesis of silver nanoparticles using Aspergillus fumigatus. The synthesis process was quite fast and silver nanoparticles were formed within minutes of silver ion coming in contact with the cell filtrate. UV-visible spectrum of the aqueous medium containing silver ion showed a peak at 420 nm corresponding to the plasmon absorbance of silver nanoparticles. Transmission electron microscopy (TEM) micrograph showed formation of well-dispersed silver nanoparticles in the range of 5-25 nm. X-ray diffraction (XRD)-spectrum of the silver nanoparticles exhibited 2theta values corresponding to the silver nanocrystal. The process of reduction being extracellular and fast may lead to the development of an easy bioprocess for synthesis of silver nanoparticles.
authors.affiliation: Enzyme and Microbial Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
authors.forename: Kuber C
authors.initials: KC
authors.lastname: Bhainsa
authors.forename: S F
authors.initials: SF
authors.lastname: D'Souza
mesh_terms: D001232:Aspergillus fumigatus / Q000254:growth & development / Q000378:metabolism*; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism*; D008567:Membranes, Artificial; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry; D012834:Silver / Q000737:chemistry* / Q000378:metabolism*; D013499:Surface Properties; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D008567:Membranes, Artificial; D012834:Silver; D012825:Silicon