Transcription of human neuronal nitric oxide synthase mRNAs derived from different first exons is partly controlled by exon 1-specific promoter sequences.
The human neuronal nitric oxide synthase (NOS1) gene is subject to extensive splicing. A total of 12 NOS1 mRNA species have been identified. They differ in their 5' ends and are derived from 12 different first exons (termed exons 1a to 1l). Various cell lines whose NOS1 first exon expression patterns were representative of human brain, skin, and skeletal muscle were identified. These included A673 neuroepithelioma cells, SK-N-MC neuroblastoma cells, HaCaT keratinocyte-like cells, and C2C12 myocyte-like cells. In these cell lines, correlations were found between the exon 1 variants preferentially expressed and the promoter activities of their cognate 5' flanking sequences. These data demonstrate that expression of the different exon 1-related splice variants of NOS1 mRNA is controlled directly (at least in part) by the associated 5' flanking sequences.
Bros
Matthias
M
Department of Dermatology, Johannes Gutenberg University, D-55101 Mainz, Germany.
Boissel
Jean-Paul
JP
Gödtel-Armbrust
Ute
U
Förstermann
Ulrich
U
D024506:5' Flanking Region; D017398:Alternative Splicing; D001665:Binding Sites; D002460:Cell Line; D003001:Cloning, Molecular; D005091:Exons; D017930:Genes, Reporter; D014644:Genetic Variation; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D012333:RNA, Messenger; D014157:Transcription Factors; D008156:Luciferases; D052248:Nitric Oxide Synthase Type I