Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells.
The damaged DNA-binding protein (DDB) complex consists of a heterodimer of p127 (DDB1) and p48 (DDB2) subunits and is believed to have a role in nucleotide excision repair (NER). We used the GAL4-UAS targeted expression system to knock down DDB1 in wing imaginal discs of Drosophila. The knock-down was achieved in transgenic flies using over-expression of inverted repeat RNA of the D-DDB1 gene [UAS-D-DDB1(650)-dsRNA]. As a consequence of RNA interference (RNAi), the fly had a shrunken wing phenotype. The wing spot test showed induced genome instability in transgenic flies with RNAi knock-down of D-DDB1 in wing imaginal discs. When Drosophila larvae with RNAi knock-down of D-DDB1 in wing imaginal discs were treated with the chemical mutagen methyl methanesulfonate (MMS), the frequency of flies with a severely shrunken wing phenotype increased compared to non-treated transgenic flies. These results suggested that DDB1 plays a role in the response to DNA damaged with MMS and in genome stability in Drosophila somatic cells.
Shimanouchi
Kaori
K
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510.
Takata
Kei-Ichi
K
Yamaguchi
Masamitsu
M
Murakami
Shizuka
S
Ishikawa
Gen
G
Takeuchi
Ryo
R
Kanai
Yoshihiro
Y
Ruike
Tatsushi
T
Nakamura
Ryoichi
R
Abe
Yoko
Y
Sakaguchi
Kengo
K
D000818:Animals; D030801:Animals, Genetically Modified; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000502:physiology*; D004330:Drosophila; D005260:Female; D042822:Genomic Instability; D008297:Male; D014921:Wings, Animal / Q000254:growth & development
D004268:DNA-Binding Proteins