Nearly neutral secretory vesicles in Drosophila nerve terminals.
The acidity of mammalian secretory vesicles drives concentration and processing of their contents. Here, pH-sensitive green fluorescent protein (GFP) variants show that the > or =30-fold (H+) difference between secretory vesicles (pH < or = 5.7) and the cytoplasm (pH = 7.2) in mammalian cells is not present in peptidergic and small synaptic vesicles of the Drosophila neuromuscular junction. First, we find that fluorescence from Topaz-tagged atrial natriuretic factor, a peptidergic vesicle pH indicator, is only modestly affected by collapsing the H+ gradient in type III synaptic boutons. Quantitation shows that peptidergic vesicles are nearly neutral (pH = 6.74 +/- 0.05), even when temperature is elevated. Furthermore, small synaptic vesicles in glutamatergic synaptic boutons, studied with synaptophluorin, are as alkaline as peptidergic vesicles. Finally, yellow fluorescent protein measurements show that cytoplasmic pH is only slightly different than in mammals (pH = 7.4). Thus, the marked acidity of mammalian secretory vesicles is not conserved in evolution, and a modest vesicular H+ gradient is sufficient for supporting neurotransmission.
Sturman
David A
DA
Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Shakiryanova
Dinara
D
Hewes
Randall S
RS
Deitcher
David L
DL
Levitan
Edwin S
ES
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000737:chemistry*; D002478:Cells, Cultured; D004331:Drosophila melanogaster / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D017729:Presynaptic Terminals / Q000737:chemistry*; D022142:Secretory Vesicles / Q000737:chemistry*; D013696:Temperature
D009320:Atrial Natriuretic Factor