title: Comprehensive gene expression profiling following DNA vaccination of rainbow trout against infectious hematopoietic necrosis virus.
abstract: The DNA vaccine based on the glycoprotein gene of Infectious hematopoietic necrosis virus induces a non-specific anti-viral immune response and long-term specific immunity against IHNV. This study characterized gene expression responses associated with the early anti-viral response. Homozygous rainbow trout were injected intra-muscularly (I.M.) with vector DNA or the IHNV DNA vaccine. Gene expression in muscle tissue (I.M. site) was evaluated using a 16,008 feature salmon cDNA microarray. Eighty different genes were significantly modulated in the vector DNA group while 910 genes were modulated in the IHNV DNA vaccinate group relative to control group. Quantitative reverse-transcriptase PCR was used to examine expression of selected immune genes at the I.M. site and in other secondary tissues. In the localized response (I.M. site), the magnitudes of gene expression changes were much greater in the vaccinate group relative to the vector DNA group for the majority of genes analyzed. At secondary systemic sites (e.g. gill, kidney and spleen), type I IFN-related genes were up-regulated in only the IHNV DNA vaccinated group. The results presented here suggest that the IHNV DNA vaccine induces up-regulation of the type I IFN system across multiple tissues, which is the functional basis of early anti-viral immunity.
authors.affiliation: School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA 98195, USA. maureenp@u.washington.edu
authors.forename: Maureen K
authors.initials: MK
authors.lastname: Purcell
authors.forename: Krista M
authors.initials: KM
authors.lastname: Nichols
authors.forename: James R
authors.initials: JR
authors.lastname: Winton
authors.forename: Gael
authors.initials: G
authors.lastname: Kurath
authors.forename: Gary H
authors.initials: GH
authors.lastname: Thorgaard
authors.forename: Paul
authors.initials: P
authors.lastname: Wheeler
authors.forename: John D
authors.initials: JD
authors.lastname: Hansen
authors.forename: Russell P
authors.initials: RP
authors.lastname: Herwig
authors.forename: Linda K
authors.initials: LK
authors.lastname: Park
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D020869:Gene Expression Profiling; D029142:Infectious hematopoietic necrosis virus / Q000276:immunology; D018482:Muscle, Skeletal / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology; D009928:Organ Specificity / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018353:Rhabdoviridae Infections / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology / Q000494:pharmacology*; D014765:Viral Vaccines / Q000276:immunology / Q000494:pharmacology*
chemical_list: D019444:Vaccines, DNA; D014765:Viral Vaccines