Tissue engineering approach to the treatment of bone tumors: three cases of cultured bone grafts derived from patients' mesenchymal stem cells.
A novel approach to the treatment of bone tumors using tissue-engineered implants is reported in this study. The number of mesenchymal stem cells (MSCs) obtained from each patient's bone marrow cells was first increased, and the MSCs were forced to differentiate into osteoblasts followed by bone matrix formation on hydroxyapatite (HA) ceramics. The strong osteogenic ability of the implants, as evidenced by high osteoblastic activity, was confirmed. Consequently, the HA surface was covered with the patient's derived cultured osteoblast/bone matrix. The tissue-engineered HA was used to fill the patient's bone cavity after tumor curettage. Immediate healing potential was found by serial plain radiographs and computed tomograhy images, and no adverse reactions were noted in these patients. The results indicate that tissue-engineered osteogenic ceramics might be an alternative to autologous bone grafts.
Morishita
Toru
T
Department of Orthopedic Surgery, National Hospital Organization Nara Medical Center, Nara, Japan. 5213web1@hosp.go.jp
Honoki
Kanya
K
Ohgushi
Hajime
H
Kotobuki
Noriko
N
Matsushima
Asako
A
Takakura
Yoshinori
Y
D000293:Adolescent; D001672:Biocompatible Materials; D001857:Bone Matrix / Q000637:transplantation*; D001859:Bone Neoplasms / Q000628:therapy*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002648:Child; D017886:Durapatite; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D008875:Middle Aged; D010006:Osteoblasts / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D016896:Treatment Outcome
D001672:Biocompatible Materials; D017886:Durapatite