title: Are sonochemically prepared alpha-amylase protein microspheres biologically active?
abstract: Using high-intensity ultrasound, we have synthesized alpha-amylase microspheres. The paper presented characterization as well as catalytic experiments of the sonochemically-produced microspheres. It also provided an estimate of the efficiency of the sonochemical process in converting the native protein to microspheres. These microspheres showed a very good enzymatic activity compared with the native alpha-amylase. The enzymatic activity of the amylase microspheres was 27% of that of the native protein after a short reaction time (3 min), while over a longer reaction time (1 h) it reached 56% of the activity of the native protein.
authors.affiliation: Department of Chemistry and Kanbar Laboratory for Nanomaterials at the Bar-Ilan University Center for Advanced Materials and Nanotechnology, Bar-Ilan University, Ramat-Gan 52900, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Avivi Levi
authors.forename: A
authors.initials: A
authors.lastname: Gedanken
mesh_terms: D004789:Enzyme Activation / Q000528:radiation effects; D004800:Enzymes, Immobilized / Q000032:analysis / Q000737:chemistry / Q000528:radiation effects*; D008863:Microspheres; D013010:Sonication; D000516:alpha-Amylases / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects*
chemical_list: D004800:Enzymes, Immobilized; D000516:alpha-Amylases