Skin-derived micro-organs induce angiogenesis in rabbits.
We have recently reported an alternative cell therapy approach to induce angiogenesis. The approach is based on small organ fragments--micro-organs (MOs)--whose geometry allows preservation of the natural epithelial/mesenchymal interactions and ensures appropriate diffusion of nutrients and gases to all cells. We have shown that lung-derived MOs, when implanted into hosts, transcribe a wide spectrum array of angiogenic factors and can induce an angiogenic response that can rescue experimentally induced ischemic regions in mice. From a clinical perspective, skin-derived MOs are particularly appealing as they could readily be obtained from a skin biopsy taken from the same target patient. In the present work we have investigated the angiogenesis-inducing capacity of rabbit and human skin-derived micro-organs in vitro and in vivo. Rabbit skin MOs were implanted into homologous adult rabbits and human skin MOs were encapsulated and implanted into xenogenic mice. Skin-derived MOs, as lung-derived MOs, were found to secrete a whole array of angiogenic factors and to induce a powerful angiogenic response when implanted back into animals. We believe the approach presented suggests a novel, efficacious and simple approach for therapeutic angiogenesis.
Hasson
Eilat
E
Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Gallula
Jeniffer
J
Shimoni
Yael
Y
Grad-Itach
Etty
E
Marikovsky
Moshe
M
Mitrani
Eduardo
E
D000328:Adult; D000818:Animals; D005260:Female; D006801:Humans; D051379:Mice; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D011817:Rabbits; D012867:Skin / Q000098:blood supply*; D016038:Skin Transplantation; D046509:Tissue Culture Techniques; D014183:Transplantation, Heterologous