Lipocalin-type prostaglandin D synthase (beta-trace) is upregulated in the alphaB-crystallin-positive oligodendrocytes and astrocytes in the chronic multiple sclerosis.
Lipocalin-type prostaglandin D synthase (L-PGDS), which is mainly synthesized in leptomeningeal cells and oligodendrocytes (OLs) in rodents and humans, is secreted into the human cerebrospinal fluid (CSF) as beta-trace. L-PGDS protects OLs and neurones against apoptosis in twitcher mice, a murine model of Krabbe's disease, and is the second only to a stress protein, alphaB-crystallin, as the most abundant gene product upregulated in the demyelinating focus of multiple sclerosis (MS). Here we report that although the CSF level of L-PGDS is not increased in MS patients, L-PGDS is increased in the white matter of MS patients, especially in the shadow plaque as compared with the normal white matter. L-PGDS immunoreactivity was intensely expressed in OLs within the shadow plaques and in hypertrophied astrocytes within the chronic plaques of MS patients. Both L-PGDS-positive OLs and astrocytes expressed a stress protein, alphaB-crystallin. These results suggest that the upregulation of L-PGDS occurs in OLs and astrocytes as a stress reaction.
Kagitani-Shimono
K
K
Department of Developmental Medicine , Pediatrics, D-5 Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Mohri
I
I
Oda
H
H
Ozono
K
K
Suzuki
K
K
Urade
Y
Y
Taniike
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001253:Astrocytes / Q000378:metabolism*; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000096:biosynthesis*; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis*; D054834:Lipocalins; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000378:metabolism* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009836:Oligodendroglia / Q000378:metabolism*; D011494:Protein Kinases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D038203:alpha-Crystallin B Chain
C059215:CRYAB protein, human; D007381:Intermediate Filament Proteins; D054834:Lipocalins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D038203:alpha-Crystallin B Chain; D011494:Protein Kinases; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase