title: In vitro evaluation of apical sealing in root apex treated with demineralization agents and retrofiled with mineral trioxide aggregate through marginal dye leakage.
abstract: The purpose of this study was to evaluate the apical seal in root apex treated with different demineralization agents and retrofilled with mineral trioxide aggregate (MTA) using marginal dye leakage. Fifty-six, human single-rooted teeth were instrumented, filled, resected and had retrofilling cavities prepared with ultrasonic tips. Demineralizing agents were applied before the apical cavities were retrofilled with Pro Root MTA. The specimens were assigned to 4 groups (n=14), as follows: group 1 (no demineralizing agent); group 2 (35% phosphoric acid, for 15 s); group 3 (17% EDTA solution, pH 7, for 3 min); and group 4 (24% EDTA gel, pH 7, for 4 min). The extension of dye (2% rhodamine B, at 37 degrees C, for 24 h) penetration was measured in millimeters using a stereomicroscope. Results were statistically analyzed by ANOVA and Tukey's test at 5% significance level. Among the experimental groups, the least extension of dye penetration was observed in group 1 (1.89 mm), followed by groups 2 (2.18 mm), 4 (2.54 mm) and 3 (2.64 mm). No statistically significant differences (p<0.05) were found in marginal microleakage among groups 1, 2 and 4 and groups 2, 3 and 4. Based on the results obtained in this study, it may be concluded that the application of demineralizing agents cannot be recommended when MTA is used in periradicular surgeries.
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry of São José dos Campos, UNESP, São José do Campos, SP, Brazil. chkubo1@ig.com.br
authors.forename: Claudio Hideki
authors.initials: CH
authors.lastname: Kubo
authors.forename: Ana Paula Martins
authors.initials: AP
authors.lastname: Gomes
authors.forename: Maria Nadir Gasparoto
authors.initials: MN
authors.lastname: Mancini
mesh_terms: D017607:Aluminum Compounds / Q000627:therapeutic use*; D001047:Apicoectomy; D017610:Calcium Compounds / Q000627:therapeutic use*; D001840:Dental Bonding; D003763:Dental Leakage / Q000145:classification*; D004338:Drug Combinations; D004492:Edetic Acid / Q000627:therapeutic use; D005456:Fluorescent Dyes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D010087:Oxides / Q000627:therapeutic use*; D010756:Phosphoric Acids / Q000627:therapeutic use; D012184:Retrograde Obturation / Q000379:methods*; D012235:Rhodamines; D012387:Root Canal Filling Materials / Q000627:therapeutic use*; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D017640:Silicates / Q000627:therapeutic use*; D013997:Time Factors; D019227:Tooth Apex / Q000187:drug effects* / Q000473:pathology; D014464:Ultrasonic Therapy / Q000295:instrumentation
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D005456:Fluorescent Dyes; D010087:Oxides; D010756:Phosphoric Acids; D012235:Rhodamines; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D017640:Silicates; C086631:mineral trioxide aggregate; D004492:Edetic Acid; C030242:phosphoric acid; C029773:rhodamine B