Fat-specific transgenic expression of resistin in the spontaneously hypertensive rat impairs fatty acid re-esterification.
OBJECTIVE
To investigate the mechanism by which fat-specific transgenic expression of resistin affects fatty acid metabolism in the spontaneously hypertensive rat (SHR).


METHODS
Basal- and adrenaline-stimulated lipolysis, basal- and insulin-stimulated lipogenesis as well as the site (glycerol versus acyl moiety) of glucose incorporated into triglycerides were determined in adipose tissue isolated from SHR-Resistin transgenic and SHR control rats.


RESULTS
A moderate expression of transgenic resistin in adipose tissue was associated with significant increase in the FFA/glycerol ratio during adrenaline-stimulated lipolysis in the SHR-Resistin transgenic rats (3.27+/-0.26) compared to SHR controls (2.11+/-0.10, P=0.0005). Transgenic SHR also exhibited a significant decrease in FFA re-esterification in adipose tissue (approximately by 23%).


CONCLUSIONS
These findings raise the possibility that the prodiabetic effects of transgenic resistin may be partly mediated by increased FFA release from adipose tissue due to impaired FFA re-esterification in adipocytes.
Pravenec
M
M
Institute of Physiology, Czech Academy of Sciences and Center for Applied Genomics, Prague, Czech Republic. pravenec@biomed.cas.cz
Kazdová
L
L
Cahová
M
M
Landa
V
V
Zídek
V
V
Mlejnek
P
P
Simáková
M
M
Wang
J
J
Qi
N
N
Kurtz
T W
TW
D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D004837:Epinephrine / Q000494:pharmacology; D004951:Esterification; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011918:Rats, Inbred SHR; D052243:Resistin / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D005230:Fatty Acids, Nonesterified; D052243:Resistin; D005947:Glucose; D005990:Glycerol; D004837:Epinephrine