title: Vaccination with DNA encoding cysteine proteinase confers protective immune response to rats infected with Clonorchis sinensis.
abstract: Cysteine proteinases of C. sinensis are important virulence factors that induce pathological changes associated with larval migration and localized biliary epithelial destruction. This study investigated the immunogenicity and protective efficacy of a DNA vaccine encoding Clonorchis sinensis cysteine proteinase (CsCP). The CsCP cDNA sequence displays significant homology to the mammalian or trematode cathepsin L. Plasmid DNA carrying the CsCP gene (pcDNA3.1-CsCP) was injected into Sprague-Dawley (SD) rats intradermally. Animals injected with pcDNA3.1-CsCP developed CsCP-specific antibodies, which exhibited an IgG2a dominance in sera. In addition, the DNA vaccine elicited the production of IFN-gamma, but not IL-4 in splenocytes, suggesting the induction of a typical Th-1 dominated immune response in rats. The pcDNA3.1-CsCP induced a significant level of protection (31.5%, p<0.05) in SD rats challenged with C. sinensis metacercariae. These results indicate that pcDNA3.1-CsCP induces both humoral and cellular immune responses. The CsCP gene may be a good candidate for use in future studies of vaccination against clonorchiasis.
authors.affiliation: Department of Biology, Daejeon University, Daejeon 300-716, South Korea.
authors.forename: Ji-Sook
authors.initials: JS
authors.lastname: Lee
authors.forename: In Sik
authors.initials: IS
authors.lastname: Kim
authors.forename: Woon-Mok
authors.initials: WM
authors.lastname: Sohn
authors.forename: Jongweon
authors.initials: J
authors.lastname: Lee
authors.forename: Tai-Soon
authors.initials: TS
authors.lastname: Yong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000920:Antibody-Dependent Cell Cytotoxicity; D003003:Clonorchiasis / Q000517:prevention & control*; D003004:Clonorchis sinensis / Q000276:immunology*; D003429:Cross Reactions; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000276:immunology; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D017422:Sequence Analysis, DNA; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D007371:Interferon-gamma; D003546:Cysteine Endopeptidases