title: [Isolation and identification of arboviruses from mosquito pools in Yunnan Province].
abstract: BACKGROUND
  To survey arboviruses in Yunnan province.
  
  
  METHODS
  Mosquitoes were collected from Yunnan Province in 2002 and 2004. Virus strains were isolated by the inoculation of homogenates of the mosquitoes onto BHK cell line. The isolated strains and their molecular biological characteristics were identified by real-time PCR, reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescent antibody technique.
  
  
  RESULTS
  Twelve strains of viruses producing CPE in BHK cells were isolated from 4810 mosquitoes. All the 12 isolates were identified to be Japanese encephalitis viruses. Genotype analysis showed the new virus (DL-0437 strain) belonged to genotype III.
  
  
  CONCLUSIONS
  Twelve strains of Japanese encephalitis viruses were isolated from mosquito pools collected in Yunnan. It was the first isolation of genotype III Japanese encephalitis viruses in Yunnan Province in recent years.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Sun
authors.forename: Shi-hong
authors.initials: SH
authors.lastname: Fu
authors.forename: Hai-lin
authors.initials: HL
authors.lastname: Zhang
authors.forename: Huan-yu
authors.initials: HY
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.forename: Wei-bin
authors.initials: WB
authors.lastname: Liu
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.forename: Ji-guang
authors.initials: JG
authors.lastname: Min
authors.forename: Rui-hong
authors.initials: RH
authors.lastname: Han
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001103:Arboviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002460:Cell Line; D002681:China; D006224:Cricetinae; D009033:Culicidae / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000821:virology; D005838:Genotype; D008856:Microscopy, Fluorescence; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C074932:prM protein, Flavivirus