PEG-derivative effectively modifies the characteristics of indomethacin-PLGA microspheres destined to intra-articular administration.
The aim of this study was to obtain biodegradable indomethacin microspheres for intra-articular administration in rheumatoid arthritis, where angiogenic processes are involved. Indomethacin concentrations to achieve an anti-angiogenic effect would be five-times higher than an anti-inflammatory. Microspheres were prepared by solvent evaporation using PLGA. Indomethacin is a poor water-soluble drug with it being possible that dissolved and non-dissolved drug co-exist within the polymeric matrix resulting in rapid release. To control this release, an oil-PEG-derivative was incorporated, producing changes in morphology, crystallinity and indomethacin release. To minimize the amount of microspheres administered, a two-factor five-level central rotable composite 2(2)+star design was employed with two independent variables: indomethacin percentage and PEG-derivative percentage. The optimum formulation showed mean encapsulation efficiency of 94.3+/-2.2% and released 7.99+/-0.25 microg indomethacin/day/mg microspheres for 21 days. A dose of 20-50 mg of this formulation could be appropriate to achieve both anti-angiogenic and anti-inflammatory effects. Preliminary cytotoxicity studies performed in rat splenocytes showed an adequate cell viability.
Puebla
Patricia
P
Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Pastoriza
Pilar
P
Barcia
Emilia
E
Fernández-Carballido
Ana
A
D018501:Antirheumatic Agents / Q000008:administration & dosage; D004334:Drug Administration Schedule; D004339:Drug Compounding / Q000379:methods; D007213:Indomethacin / Q000008:administration & dosage; D007270:Injections, Intra-Articular; D019344:Lactic Acid; D008863:Microspheres; D011092:Polyethylene Glycols; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers
D018501:Antirheumatic Agents; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; D007213:Indomethacin