A novel test for identifying genes involved in aldehyde detoxification in the yeast. Increased sensitivity of superoxide-deficient yeast to aldehydes and their metabolic precursors.
A novel test for the identification of genes involved in aldehyde metabolism is proposed, based on detection of altered sensitivity of the yeast to corresponding alcohols, metabolic precursors of the aldehydes. This attitude enabled to an unexpected detection increased sensitivity of mutants devoid of CuZn-superoxide dismutase (CuZnSOD) to allyl alcohol (precursor of acrolein) and nonenol. We interpret this finding as due to inactivation of some important element of aldehyde detoxification by increased flux of superoxide in DeltaCuZnSOD mutants.
Biliński
Tomasz
T
Department of Biochemistry and Cell Biology, University of Rzeszów, Poland. bilinski@univ.rzeszow.pl
Kwolek
Magdalena
M
Sas
Ewelina
E
Krynicka
Marta
M
Koziol
Sabina
S
Owsiak-Teleon
Alina
A
Krzepilko
Anna
A
Bartosz
Grzegorz
G
D000171:Acrolein / Q000494:pharmacology; D000447:Aldehydes / Q000378:metabolism* / Q000494:pharmacology*; D005978:Glutathione / Q000502:physiology; D008658:Inactivation, Metabolic; D009154:Mutation; D020005:Propanols / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000502:physiology; D000072105:Superoxide Dismutase-1; D013481:Superoxides / Q000378:metabolism*
D000447:Aldehydes; D020005:Propanols; D013481:Superoxides; C006463:allyl alcohol; D000171:Acrolein; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D005978:Glutathione