title: Determination of 1-nitropyrene metabolites by high-performance liquid chromatography with chemiluminescence detection.
abstract: Three metabolites of 1-nitropyrene, i.e., 3-hydoxy-1-nitropyrene (3-OHNP), 6-hydoxy-1-nitropyrene (6-OHNP) and 8-hydoxy-1-nitropyrene (8-OHNP), were sensitively and selectively detected by HPLC with peroxyoxalate chemiluminescence (CL) detection. In the system, the three OHNPs were reduced to their corresponding amino derivatives through a reduction column packed with Pt/Rh-coated alumina and then concentrated on a concentrator column. By rotating a switching valve, the analytes were eluted into a separator column (ODS), separated and then detected by the bis(2,4,6-trichlorphenyl)oxalate-H(2)O(2) CL system. The detection limits (signal-to-noise ratio=3) were in the range from 5fmol (6-OHNP and 8-OHNP) to 12fmol (3-OHNP) per injection. To demonstrate the proposed method, it was used to determine three compounds in the incubation mixture of 1-nitropyrene and rat S9 mix.
authors.affiliation: Graduate School of Natural Science and Technology, Faculty of Pharmaceutical Sciences, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.forename: Satoko
authors.initials: S
authors.lastname: Mizukami
authors.forename: Akira
authors.initials: A
authors.lastname: Toriba
authors.forename: Ning
authors.initials: N
authors.lastname: Tang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008163:Luminescent Measurements; D011721:Pyrenes / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D011721:Pyrenes; C032668:1-nitropyrene