Fewer PrPc myeloid-based cells in sheep with the prion-resistant genotype.
Numerous sheep with the prion gene (PRNP) that encodes for 171QQ develop scrapie, a neurodegenerative prion disease of sheep. Relatively few cases of scrapie-affected sheep with PRNP genetics that encode for 171RR, however, have been found. Using flow cytometric analysis, statistically fewer PrPc-positive microglia and monocytes were observed from sheep with 171RR PRNP genetics than from sheep with 171QQ PRNP genetics (P<0.05). One possibility for the lack of PrP(Sc) accumulation in brains and lymph nodes of scrapie-exposed sheep with 171RR PRNP genetics is because of fewer PrPc-positive myeloid-derived cells available for conversion of PrPc to PrP(Sc).
Herrmann
Lynn M
LM
Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, USA. lherrman@vetmed.wsu.edu
Baszler
Timothy V
TV
O'Rourke
Katherine I
KI
Suarez
Carlos E
CE
Bakko
Marlene
M
Alverson
Janet
J
Knowles
Donald P
DP
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005838:Genotype; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D017628:Microglia / Q000473:pathology / Q000648:ultrastructure; D009000:Monocytes / Q000378:metabolism; D022423:Myeloid Cells / Q000378:metabolism* / Q000648:ultrastructure; D037121:Plant Lectins / Q000378:metabolism; D018621:PrPC Proteins / Q000378:metabolism* / Q000472:pathogenicity; D011328:Prions / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012608:Scrapie / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D012756:Sheep
D005904:Glial Fibrillary Acidic Protein; D037121:Plant Lectins; D018621:PrPC Proteins; D011328:Prions; D012333:RNA, Messenger; C017707:Ricinus communis agglutinin-1