title: Dynamic ultrasound-assisted extraction of polyphenols in tobacco.
abstract: A novel extraction method, namely dynamic ultrasound-assisted extraction, is investigated. This technique is efficient with respect to both time and solvent consumption because it utilizes ultrasonic energy in dynamic mode during extraction. Polyphenols (chlorogenic acid, esculetin, rutin, scopoletin, and quercitrin) are extracted from a tobacco (Nicotina tobaccum L.) sample for 10 min with 6 mL of solvent. Fresh solvent is continuously pumped through the sample, with which the analytes can be rapidly extracted, and the possibility of degradation efficiently avoided. Methanol involving 0.5% w/v ascorbic acid was used as extraction solvent; optimal flow rate and extraction time were investigated. The extract was cleaned up by C18 disposable cartridge. The spiked and nonspiked tobacco samples were used for the evaluation of the proposed method. Recoveries obtained were varied from 96 to 108% and RSDs from 2.0 to 4.6%. This extraction technique was revealed to recover larger amounts of polyphenols from tobacco, compared to the static ultrasound-assisted extraction method.
authors.affiliation: Department of Chemistry, University of Science & Technology of China, Hefei, China.
authors.forename: Xungang
authors.initials: X
authors.lastname: Gu
authors.forename: Jibao
authors.initials: J
authors.lastname: Cai
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Zhu
authors.forename: Qingde
authors.initials: Q
authors.lastname: Su
mesh_terms: D002244:Carbon / Q000737:chemistry; D002621:Chemistry / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D000432:Methanol / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D059808:Polyphenols; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D014026:Nicotiana / Q000737:chemistry*; D014465:Ultrasonics
chemical_list: D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D012997:Solvents; D002244:Carbon; D000432:Methanol