title: Functional interplay between the jaw domain of bacterial RNA polymerase and allele-specific residues in the product RNA-binding pocket.
abstract: Bacterial RNA polymerase (RNAP) is a complex molecular machine in which the network of interacting parts and their movements, including contacts to nascent RNA and the DNA template, are at best partially understood. The jaw domain is a part of RNAP that makes a key contact to duplex DNA as it enters the enzyme from downstream and also contacts two other parts of RNAP, the trigger loop, which lies in the RNAP secondary channel, and a sequence insertion in the Escherichia coli RNAP trigger loop that forms an external domain and also contacts downstream DNA. Deletion of the jaw domain causes defects in transcriptional pausing and in bacterial growth. We report here that these defects can be partially corrected by a limited set of substitutions in a distant part of RNAP, the product RNA-binding pocket. The product RNA-binding pocket binds nascent RNA upstream of the active site and is the binding site for the RNAP inhibitor rifampicin when RNA is absent. These substitutions have little effect on transcript elongation between pause sites and actually exacerbate jaw-deletion defects in transcription initiation, suggesting that the pausing defects may be principally responsible for the in vivo phenotype of the jaw deletion. We suggest that the counteracting effects on pausing of the alterations in the jaw and the product RNA binding site may be mediated either by effects on translocation or via allosteric communication to the RNAP active site.
authors.affiliation: Department of Genetics Microbiology & Toxicology, Stockholm University, SE-10691 Stockholm, Sweden.
authors.forename: Josefine
authors.initials: J
authors.lastname: Ederth
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Mooney
authors.forename: Leif A
authors.initials: LA
authors.lastname: Isaksson
authors.forename: Robert
authors.initials: R
authors.lastname: Landick
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010641:Phenotype; D011487:Protein Conformation; D012313:RNA / Q000378:metabolism*; D016415:Sequence Alignment; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases