[Study on the conditions of high density fermentation for the engineering bacterial strain with RGD spider silk protein gene].
Spider silk becomes the protein fibroin with excellent character in the natural world because of its unique mechanical properties. The genetic engineering technique is an effective method to procure the spider silk protein. In order to obtain RGD-spider silk protein gene recombinant engineering strain pNSR-16 on a regular scale, we determine the optimal conditions of the growth and expression of pNSR-16 through culture in laboratory, and based on these, study the fed-batch high density culture. We control the flow of carbon-nitrogen, the dissolved oxygen concentration and the cell specific growth rate, which make the final cell density and concentration of recombinant protein of total protein arrive at OD600 57.15 and 20.8%, respectively.
Li
Min
M
College of Bioengineering, Fujian Normal University, Fuzhou 350007, China. mli@fjnu.edu.cn
Tu
Guiyun
G
Huang
Zhihua
Z
Huang
Xi
X
D000818:Animals; D001419:Bacteria / Q000235:genetics; D003001:Cloning, Molecular; D005285:Fermentation; D005349:Fibroins / Q000096:biosynthesis* / Q000235:genetics; D005818:Genetic Engineering; D009842:Oligopeptides / Q000235:genetics; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics*
D009842:Oligopeptides; D011994:Recombinant Proteins; C047981:arginyl-glycyl-aspartic acid; D005349:Fibroins