Plant stanol ascorbate esters reduce body weight gain through decreased energy absorption in hamsters.
OBJECTIVE
The objective of this study was to determine the effects of disodium ascorbyl phytostanyl phosphate (DAPP), a novel hydrophilic phytostanol analogue, on energy homeostasis, including body weight and intestinal energy absorption, and plasma triglyceride concentrations, in hamsters.


METHODS
Male Golden Syrian hamsters (n = 50) were fed for 5 weeks with experimental diets varying in cholesterol and phytostanol content. Diets included (i) non-cholesterol (semipurified diet without added cholesterol), (ii) cholesterol-control (semipurified diet with 0.25% cholesterol), (iii) stanol (cholesterol-control with 1% free phytostanols), (iv) DAPP 0.7% (cholesterol-control with 0.71% DAPP) or (v) DAPP 1.4% (cholesterol-control with 1.43% DAPP). Fecal samples were collected continuously for 3 days on week 3, and fecal energy output was measured by bomb calorimetry.


RESULTS
Hamsters fed 1.4% DAPP gained less (P<0.05) weight than hamsters fed non-cholesterol and stanol diets. Diets had no effect on total food consumption or gross energy intake after 5 weeks, but lower (P<0.05) weekly food consumptions in hamsters fed 1.4% DAPP were observed at weeks 1 and 2 of the experiment in comparison to animals fed the non-cholesterol diet. In comparison to non-cholesterol and cholesterol-control diets, DAPP 1.4% increased (P<0.01) fecal energy output by 47 and 46%, respectively. In hamsters supplemented with 1.4% DAPP, plasma triglyceride concentrations were 45% lower (P<0.05) than in cholesterol-control fed hamsters. Furthermore, plasma triglyceride levels in the DAPP 1.4% group was 49% lower (P<0.01) than in the stanol group, despite the fact that both diets contained equivalent amounts of phytostanols. The lower concentration of DAPP (0.7%) also reduced plasma triglycerides (P<0.05) compared with the stanol diet.


CONCLUSIONS
Stanol-ascorbate decreases body weight gain in hamsters, likely due to lower energy absorption at the intestinal level. In addition to its previously observed powerful cholesterol-lowering effect, DAPP has a hypotriglyceridemic function in hamsters.
Ebine
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School of Dietetics and Human Nutrition, McGill University, Macdonald Campus, 21,111 Lakeshore, Ste-Anne-de-Bellevue, QC, Canada.
Demonty
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I
Jia
X
X
Jones
P J H
PJ
D000818:Animals; D002151:Calorimetry; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D006224:Cricetinae; D004038:Diet, Reducing; D004435:Eating / Q000187:drug effects; D004734:Energy Metabolism; D005243:Feces / Q000737:chemistry; D000960:Hypolipidemic Agents / Q000627:therapeutic use*; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism; D010840:Phytosterols / Q000627:therapeutic use; D011897:Random Allocation; D012855:Sitosterols / Q000627:therapeutic use*; D014280:Triglycerides / Q000097:blood
D002791:Cholesterol, Dietary; D000960:Hypolipidemic Agents; D010840:Phytosterols; D012855:Sitosterols; D014280:Triglycerides; C407042:plant stanol ester