A dynamic model of Rubisco turnover in cereal leaves.
A simple, improved model of Rubisco synthesis and degradation in cereal leaves is developed using data obtained over the leaf lifespan to return maximum likelihood values for Rubisco proteolysis and biosynthesis. It assumes that the time course of leaf Rubisco content can be described using a log-normal curve, and degradation of the Rubisco pool occurs exponentially. Curve parameters give an insight into how Rubisco dynamics differ among treatments or genotypes; also, statistical analyses can be performed more easily, requiring fewer data and allowing more flexibility in sampling than previous studies. Predicted patterns of synthesis correlate well with independent rbc gene transcript data. Rubisco degradation is a first-order decay process, and biosynthesis correlates with leaf elongation rates. As Rubisco degradation takes place according to first-order kinetics, control of leaf Rubisco concentration must be exerted by adjustment of biosynthetic, rather than proteolytic, rates.
Irving
Louis John
LJ
School of Biological Sciences, University of Aberdeen, Cruickshank Building, St Machar Drive, Aberdeen AB24 3UU, UK. L.J.Irving@massey.ac.nz
Robinson
David
D
D003627:Data Interpretation, Statistical; D002523:Edible Grain / Q000201:enzymology* / Q000254:growth & development; D005838:Genotype; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology* / Q000254:growth & development; D012333:RNA, Messenger / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*
D012333:RNA, Messenger; D012273:Ribulose-Bisphosphate Carboxylase; D009584:Nitrogen