title: Transduction of nondividing human macrophages with gammaretrovirus-derived vectors.
abstract: It is commonly accepted that infection of nondividing cells by gammaretroviruses such as the murine leukemia viruses is inefficient due to their inability to cross the nuclear envelope barrier. Challenging this notion, we now show that human nondividing macrophages display a specific window of susceptibility to transduction with a Friend murine leukemia virus (F-MLV)-derived vector during their differentiation from monocytes. This finding suggests that factors other than the nuclear membrane govern permissiveness to gammaretroviral infection and raises the possibility of using the macrophage tropism of F-MLV in gene therapy.
authors.affiliation: LaboRetro, INSERM U412, Ecole Normale Supérieure de Lyon, IFR 128 BioSciences Lyon-Gerland, 46 Allée d'Italie, 69364 Lyon, France.
authors.forename: Loraine
authors.initials: L
authors.lastname: Jarrosson-Wuilleme
authors.forename: Caroline
authors.initials: C
authors.lastname: Goujon
authors.forename: Jeanine
authors.initials: J
authors.lastname: Bernaud
authors.forename: Dominique
authors.initials: D
authors.lastname: Rigal
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Darlix
authors.forename: Andrea
authors.initials: A
authors.lastname: Cimarelli
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D049109:Cell Proliferation; D004279:DNA, Viral / Q000235:genetics; D005622:Friend murine leukemia virus / Q000235:genetics*; D015316:Genetic Therapy; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000821:virology*; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D009685:Nuclear Envelope / Q000821:virology; D011994:Recombinant Proteins; D014161:Transduction, Genetic
chemical_list: D004279:DNA, Viral; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor