Reduction kinetics of As (V) to As (III) by a dissimilatory arsenate-reducing bacterium, Bacillus sp. SF-1.
This study proposes a kinetic model that accounts for the toxicity of both arsenate and arsenite and characterizes the arsenate reduction ability of a dissimilatory arsenate-reducing bacterium, Bacillus sp. SF-1 as a bioremediation agent. The model results correlated well with a series of batch reduction experiments conducted anaerobically in serum bottles with initial arsenate concentrations of 360, 735, and 1,500 mg-As/L. The reduction rate was expressed by the Haldane equation that describes the inhibitory effect of high concentrations of arsenate. The reduction rate constant k(r), half saturation constant K(S), and inhibition constant K(I) were estimated respectively as 1.2 x 10(9) mg-As/cells/h, 1.5 x 10(2) mg-As/L, and 4.2 x 10(2) mg-As/L. Lethal effects of arsenite that is accumulated as the end-product of arsenate reduction were expressed by the first-order term with a lethal constant of 2.7 x 10(-4) L/mg-As/h. The yield for the bacterial cells by arsenate respiration was estimated at 4.0 x 10(8) cells/mg-As.
Soda
Satoshi O
SO
Department of Global Architecture, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan. soda@ga.eng.osaka-u.ac.jp
Yamamura
Shigeki
S
Zhou
Hong
H
Ike
Michihiko
M
Fujita
Masanori
M
D001149:Arsenates / Q000378:metabolism* / Q000633:toxicity; D001151:Arsenic; D018053:Arsenites / Q000633:toxicity; D001407:Bacillus / Q000187:drug effects / Q000378:metabolism*; D001673:Biodegradation, Environmental; D007700:Kinetics; D008954:Models, Biological; D010084:Oxidation-Reduction
D001149:Arsenates; D018053:Arsenites; C015001:arsenite; D001151:Arsenic; C025657:arsenic acid