Differences in Hsp70 expression in the sporozoites of the original strain and precocious lines of Eimeria tenella.
The levels of expression of Heat shock protein 70 (Hsp 70) in sporozoites of a wild-type parent strain and 2 precocious lines of Eimeria tenella, were compared to investigate the relationship between the heat shock proteins expressed by the parasite and virulence of the strain. Hsp70 expression was analyzed in sporozoites by immunohistochemical techniques, immunoblot, and flow cytometric analyses. One band of 70 kDa was identified and the variation of the Hsp70 expression levels was quantified by optical densitometric analyses. The results showed a significant gradual decrease in the Hsp70 expression in sporozoites of E. tenella as attenuation progressed, suggesting that the Hsp70 expressed in the excysted sporozoites of E. tenella might be involved in parasite pathogenicity. In addition, the cytoplasmic distribution of the Hsp70, which was observed in the entire sporozoites of the wild strain, was reduced to the anterior portion in the precocious lines.
del Cacho
E
E
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Spain. edelcach@unizar.es
Gallego
M
M
López-Bernad
F
F
Quílez
J
J
Sánchez-Acedo
C
C
D000818:Animals; D002645:Chickens / Q000469:parasitology; D003048:Coccidiosis / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary; D003720:Densitometry / Q000662:veterinary; D016786:Eimeria tenella / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry / Q000662:veterinary; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000276:immunology; D015151:Immunoblotting / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D011201:Poultry Diseases / Q000469:parasitology / Q000517:prevention & control; D016052:Protozoan Vaccines; D034101:Sporozoites / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014613:Vaccines, Attenuated
D018840:HSP70 Heat-Shock Proteins; D016052:Protozoan Vaccines; D014613:Vaccines, Attenuated