title: Proteome survey reveals modularity of the yeast cell machinery.
abstract: Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. Here we report the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry. Through systematic tagging of open reading frames (ORFs), the majority of complexes were purified several times, suggesting screen saturation. The richness of the data set enabled a de novo characterization of the composition and organization of the cellular machinery. The ensemble of cellular proteins partitions into 491 complexes, of which 257 are novel, that differentially combine with additional attachment proteins or protein modules to enable a diversification of potential functions. Support for this modular organization of the proteome comes from integration with available data on expression, localization, function, evolutionary conservation, protein structure and binary interactions. This study provides the largest collection of physically determined eukaryotic cellular machines so far and a platform for biological data integration and modelling.
authors.affiliation: Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors.forename: Anne-Claude
authors.initials: AC
authors.lastname: Gavin
authors.forename: Patrick
authors.initials: P
authors.lastname: Aloy
authors.forename: Paola
authors.initials: P
authors.lastname: Grandi
authors.forename: Roland
authors.initials: R
authors.lastname: Krause
authors.forename: Markus
authors.initials: M
authors.lastname: Boesche
authors.forename: Martina
authors.initials: M
authors.lastname: Marzioch
authors.forename: Christina
authors.initials: C
authors.lastname: Rau
authors.forename: Lars Juhl
authors.initials: LJ
authors.lastname: Jensen
authors.forename: Sonja
authors.initials: S
authors.lastname: Bastuck
authors.forename: Birgit
authors.initials: B
authors.lastname: Dümpelfeld
authors.forename: Angela
authors.initials: A
authors.lastname: Edelmann
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Heurtier
authors.forename: Verena
authors.initials: V
authors.lastname: Hoffman
authors.forename: Christian
authors.initials: C
authors.lastname: Hoefert
authors.forename: Karin
authors.initials: K
authors.lastname: Klein
authors.forename: Manuela
authors.initials: M
authors.lastname: Hudak
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Michon
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Schelder
authors.forename: Markus
authors.initials: M
authors.lastname: Schirle
authors.forename: Marita
authors.initials: M
authors.lastname: Remor
authors.forename: Tatjana
authors.initials: T
authors.lastname: Rudi
authors.forename: Sean
authors.initials: S
authors.lastname: Hooper
authors.forename: Andreas
authors.initials: A
authors.lastname: Bauer
authors.forename: Tewis
authors.initials: T
authors.lastname: Bouwmeester
authors.forename: Georg
authors.initials: G
authors.lastname: Casari
authors.forename: Gerard
authors.initials: G
authors.lastname: Drewes
authors.forename: Gitte
authors.initials: G
authors.lastname: Neubauer
authors.forename: Jens M
authors.initials: JM
authors.lastname: Rick
authors.forename: Bernhard
authors.initials: B
authors.lastname: Kuster
authors.forename: Peer
authors.initials: P
authors.lastname: Bork
authors.forename: Robert B
authors.initials: RB
authors.lastname: Russell
authors.forename: Giulio
authors.initials: G
authors.lastname: Superti-Furga
mesh_terms: D016681:Genome, Fungal; D046912:Multiprotein Complexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D046912:Multiprotein Complexes; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins