title: No relationship between IL-1B gene polymorphism and gastric acid secretion in younger healthy volunteers.
abstract: OBJECTIVE
  To investigate the influence of IL-1B-511 gene polymorphism on IL-1B mRNA expression and gastric acid output in individual with or without Helicobacter pylori (H pylori) infection.
  
  
  METHODS
  IL-1B mRNA expression and gastric acid secretion in 117 health volunteers were assayed using semi-quantitative RT-PCR and gastric juice assay, respectively. Pepsinogen (PG) I and II of 255 subjects (including 117 health volunteers) were also examined.
  
  
  RESULTS
  T/T genotype individuals with H pylori infection had a more decreased PG I/II ratio. In gastric antrum mucosa, the individuals with H pylori infection had higher IL-1B expression than those without H pylori infection, but there was no obvious difference among each genotype. In gastric corpus, the individuals with H pylori infection had a significantly higher IL-1B expression than those without H pylori infection. IL-1B-511T/T genotype was markedly higher as compared with the other two genotypes. Both maximal acid output and basic acid output were similar among each genotype in IL-1B-511 gene locus, regardless of H pylori infection.
  
  
  CONCLUSIONS
  IL-1B-511 T allele does not decrease gastric acid output, although it has a stimulated influence on IL-1B expression. Consequently, the pathway, through which IL-1B plays a central role in gastric cancer development, might not depend on low acid, but on the other regulation mechanisms.
authors.affiliation: Institute of Cancer, the Cancer Hospital of Hubei Province, Wuhan 430079, Hubei Province, China. ehusmn@yahoo.com.cn
authors.forename: Sheng
authors.initials: S
authors.lastname: Hu
authors.forename: Qi-Bing
authors.initials: QB
authors.lastname: Song
authors.forename: Ping-Fang
authors.initials: PF
authors.lastname: Yao
authors.forename: Qing-Long
authors.initials: QL
authors.lastname: Hu
authors.forename: Ping-Jin
authors.initials: PJ
authors.lastname: Hu
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Zeng
authors.forename: Rui-Ping
authors.initials: RP
authors.lastname: Pang
mesh_terms: D000328:Adult; D005260:Female; D005744:Gastric Acid / Q000378:metabolism*; D016481:Helicobacter Infections / Q000378:metabolism / Q000503:physiopathology*; D016480:Helicobacter pylori; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D013270:Stomach / Q000382:microbiology / Q000502:physiology*
chemical_list: D007375:Interleukin-1