[Effect of the Yifuning soft gelatin capsules on estrogen receptor and nitric oxide levels in ovariectomized rats].
OBJECTIVE
To investigate the effects of the Yifuning soft gelatin capsules(YFN)on estrogen receptor (ER) and nitric oxide (NO) levels in ovariectomized rats.


METHODS
Fifty female mature sprague-dawley rats were randomly divided into 5 groups: normal control; model control; diethylstilbestrol tablets (DT); YFN (high dose and low dose). After 4 weeks' treatment, the serum E2 levels were detected by radioimmunoassay. The estrogen receptor (ER) levels of uterus and artery were detected with method of radioligand binding assay of receptor (RBAR). The uterus pathologic changes were investigated with light microscope. NO and NOS levels of the artery and the uterus index were detected too.


RESULTS
YFN can obviously improve serum E2, increase index and ER of uterus (P < 0.01 or P < 0.05), and ameliorate the uterus' pathologic changes in OVX rats. It also can increase the artery' ER, NO and NOS levels.


CONCLUSIONS
YFN can cure the climacteric syndrome and prevent the cardiovascular disease after post-menopause.
Xiao
Wei
W
Zhu Jiang Hospital, Southern Medicine University, Guangzhou 510282, China. myxw1977@fim-mu.com
Ma
Yun
Y
Deng
Hong-zhu
HZ
Wei
Lian-bo
LB
D000818:Animals; D001011:Aorta / Q000378:metabolism; D002214:Capsules; D030024:Curcuma / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D004958:Estradiol / Q000097:blood; D005260:Female; D008419:Materia Medica / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010052:Ovariectomy; D010057:Oviducts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011896:Rana temporaria; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism / Q000473:pathology*
D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D008419:Materia Medica; D011960:Receptors, Estrogen; D009569:Nitric Oxide; D004958:Estradiol; D019001:Nitric Oxide Synthase