A new type of anti-ganglioside antibodies present in neurological patients.
High titers of anti-GA1 antibodies have been associated with neurological syndromes. In most cases, these antibodies cross-react with the structurally related glycolipids GM1 and GD1b, although specific anti-GA1 antibodies have also been reported. The role of specific anti-GA1 antibodies is uncertain since the presence of GA1 in the human nervous system has not been clarified. A rabbit was immunized with GD1a and its sera were screened for antibody reactivity by standard immunoassay methods (HPTLC-immunostaining and ELISA). Anti-GD1a antibodies were not detected but, unexpectedly, anti-GA1 IgG-antibodies were found. Antibody binding to GA1 was inhibited by soluble GA1 but also by GD1a. These results indicate that the rabbit produced antibodies that recognize epitopes present on the glycolipids, that are absent or not exposed on solid phase adsorbed GD1a. We investigated the presence of these unusual anti-ganglioside antibodies in normal and neurological patient sera. Approximately, 10% of normal human sera contained low titer of specific anti-GA1 IgG-antibodies but none of them recognized soluble GD1a. High titers of IgG-antibodies reacting only with GA1 were detected in 12 patient sera out of 325 analyzed. Of these, 6 sera showed binding that was inhibited by soluble GD1a and four of them also by GM1. This new type of anti-ganglioside antibodies should be considered important elements for understanding of the pathogenesis of these diseases as well as their diagnosis.
Lopez
Pablo H H
PH
Departamento de Química Biológica Dr. Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba and CIQUIBIC, CONICET, Ciudad Universitaria, Cordóba-5000, Argentina.
Comín
Romina
R
Villa
Andres M
AM
Di Egidio
Mariana
M
Saizar
Roberto D
RD
Sica
Roberto E P
RE
Nores
Gustavo A
GA
D000818:Animals; D000906:Antibodies / Q000097:blood*; D005732:Gangliosides / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D008958:Models, Molecular; D009422:Nervous System Diseases / Q000097:blood / Q000276:immunology; D011487:Protein Conformation; D011817:Rabbits
D000906:Antibodies; D005732:Gangliosides