Study of recombinant human osteogenic protein-1 expressed in prokaryocyte on the repair of extracted socket in rabbits.
The study investigated the effect of recombinant human osteogenic protein-1 (rhOP-1) expressed in prokaryocyte, to promote the healing of alveolar socket. A model of rabbit extracted socket into which the composites of rhOP-1 and gelatin sponge was immediately implanted was created and the osteoinduction of rhOP-1 was assessed by histological method, quantitative measurement of calcium content and alkaline phosphatase (ALP) activity. The result of histology showed that bone healing in rhOP-1 side is 4-6 weeks earlier than that of the control side. ALP activity and calcium content in rhOP-1 side were significantly high compared with that of the control side. rhOP-1 has a satisfactory osteoinduction ability to promote the healing of extracted socket.
Liu
Yi
Y
Key Laboratory for Biotech-Drugs Ministry of Health, Shandong Medicinal Biotechnology Center, Jinan 250062, People's Republic of China.
Han
Jin-Xiang
JX
Xiao
Shui-Qing
SQ
Wang
Shi-Li
SL
Wang
Min
M
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism*; D001861:Bone Regeneration / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D017102:Fracture Healing; D050723:Fractures, Bone; D006801:Humans; D008338:Mandibular Injuries / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D011817:Rabbits; D011897:Random Allocation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D020390:Tooth Socket / Q000166:cytology / Q000473:pathology / Q000502:physiology
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D000469:Alkaline Phosphatase; D002118:Calcium