title: Release kinetics of ampicillin, characterization and bioactivity of TiO2/PCL hybrid materials synthesized by sol-gel processing.
abstract: Poly(epsilon-caprolactone) (PCL 6, 12, and 24 wt %) and titanium (TiO2) organic-inorganic hybrid materials have been synthesized by the sol-gel method from a multicomponent solution containing titanium butoxide, poly(epsilon-caprolactone) (PCL), water, and chloroform (CHCl3). Sodium ampicillin was incorporated in the hybrid material to verify the effect as local controlled drug delivery system. The structure of a hybrid materials interpenetrating network is realized by hydrogen bonds between Ti-OH group (H-donator) in the sol-gel intermediate species and carboxylic group (H-acceptor) in the repeating units of the polymer. The presence of hydrogen bonds between organic/inorganic components of the hybrid materials was proved by FTIR analysis. The morphology of the hybrid materials was studied by scanning electron microscope (SEM). The structure of a molecular level dispersion has been disclosed by atomic force microscope (AFM), pore size distribution and surface measurements. The bioactivity of the synthesized hybrid materials has been showed by the formation of a layer of hydroxyapatite on the surface of TiO2/PCL samples soaked in a fluid simulating the composition of the human blood plasma. The amount of sodium ampicillin released has been detected by UV-vis spectroscopy and SEM. The release kinetics seems to occur in more than one stage. HPLC analysis has also been taken to ensure the integrity of ampicillin after the synthetic treatment.
authors.affiliation: Department of Aerospace and Mechanical Engineering, Second University of Naples, Via Roma no. 21, 81031 Aversa (CE), Italy. michelina.catauro@unina2.it
authors.forename: M
authors.initials: M
authors.lastname: Catauro
authors.forename: M G
authors.initials: MG
authors.lastname: Raucci
authors.forename: D
authors.initials: D
authors.lastname: de Marco
authors.forename: L
authors.initials: L
authors.lastname: Ambrosio
mesh_terms: D000667:Ampicillin / Q000493:pharmacokinetics*; D001672:Biocompatible Materials / Q000737:chemistry / Q000493:pharmacokinetics*; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D005782:Gels / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D011091:Polyesters / Q000737:chemistry / Q000493:pharmacokinetics*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D005782:Gels; D011091:Polyesters; C009495:titanium dioxide; C016240:polycaprolactone; D000667:Ampicillin; D014025:Titanium