[Determination of hedychenone in Hedychium yunnanense].
OBJECTIVE
To establish an HPLC method for determination of hedychenone in Hedychium yunnanense.


METHODS
C18 Chromatographic column was used, acetonitrile-water (9:1) as mobile phase, at flow rate of 1.0 mL x min(-1): The wavelength for detection was 235 nm.


RESULTS
The linear range of hedychenone was 5.92-29.6 microg x mL(-1)(r = 0.9999). The average recovery was 99.0%, RSD of precision was less than 2%.


CONCLUSIONS
The method is simple, effective and feasible, and can be used to evaluate the quality of the herb.
Yan
Qi-xin
QX
School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510000, China. yanqixin@eyou.com
Ye
Xiao-wen
XW
Zhao
Qing
Q
Zhao
Jin-hua
JH
Lin
Yong-cheng
YC
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004224:Diterpenes / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D030521:Zingiberaceae / Q000737:chemistry*
D004224:Diterpenes; C517485:hedychenone