Synthesis and photo-induced DNA cleaving activities of conjugates of halophenyl derivative and polyamide containing N-methylimidazoles.
Halophenyl derivatives were linked to different termini of polyamide containing three imidazole rings to give novel conjugates for examining the influence of the location of linking on DNA-cleavage efficiency. The DNA cleavage activities of the conjugates were compared on supercoiled DNA (pBluescript SK) under UV irradiation at 365 nm. It was found that bromophenyl-ImImImbetaDp (2), which is the conjugate of bromophenyl motif directly linked to the N-terminus of polyamide, had the highest photocleavage efficiency among six novel conjugates. In addition, it was found that the polyamide containing three N-methylimidazole rings could greatly enhance the photocleavage efficiency of these conjugates.
Liu
Dan
D
Department of Chemical Biology, The Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry, Peking University, Beijing 100871, PR China.
Yuan
Gu
G
Hu
Jiemiao
J
D004247:DNA / Q000737:chemistry / Q000528:radiation effects*; D006219:Halogens / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009757:Nylons / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D014466:Ultraviolet Rays
D006219:Halogens; D007093:Imidazoles; D009757:Nylons; D004247:DNA; C018100:1-methylimidazole