title: Decreased keratinocyte motility in skin wound on mice lacking the epidermal fatty acid binding protein gene.
abstract: Fatty acids are shown to be important in various skin functions. Fatty acid binding protein (FABP) is postulated to serve as a lipid shuttle, solubilizing hydrophobic fatty acids and delivering them to the appropriate metabolic system. Among the FABP family proteins, epidermal-type FABP (E-FABP) is solely expressed in keratinocyte but its specific role in skin is not yet fully established. We found an elevated expression of E-FABP in regenerative keratinocytes of healing wounds. However, E-FABP null mice showed no marked differences compared to wild type mice in the process of wound closure, in vivo. On the other hand, in keratinocyte culture, E-FABP gene disruption decreased the cell motility, but did not affect the cell proliferation. E-FABP deletion may be compensated for in vivo by the microenvironment comprised of various cells such as fibroblasts and endothelial cells around the wound. Our analyses suggest that the E-FABP elevation may be necessary for the activation of cell motility within regenerative epidermis during wound healing.
authors.affiliation: Department of Dermatology, Tohoku University Graduate School of Medicine, Tohoku, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kusakari
authors.forename: Eisaku
authors.initials: E
authors.lastname: Ogawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Owada
authors.forename: Noriko
authors.initials: N
authors.lastname: Kitanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Michiyo
authors.initials: M
authors.lastname: Kimura
authors.forename: Hachiro
authors.initials: H
authors.lastname: Tagami
authors.forename: Hisatake
authors.initials: H
authors.lastname: Kondo
authors.forename: Setsuya
authors.initials: S
authors.lastname: Aiba
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Okuyama
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D004817:Epidermis / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015603:Keratinocytes / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D012867:Skin / Q000293:injuries* / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D050556:Fatty Acid-Binding Proteins