title: Kaposi's sarcoma-associated herpesvirus viremia is associated with the progression of classic and endemic Kaposi's sarcoma.
abstract: In order to gain further insight on the role of Kaposi's sarcoma-associated herpesvirus (KSHV) in classic and endemic Kaposi's sarcoma (KS) pathogenesis, we aimed to determine (i) whether KSHV is detectable in peripheral blood mononuclear cells (PBMCs), (ii) which PBMCs subpopulation harbor the virus, (iii) which clinical, histologic, and immunologic parameters are associated with KSHV viremia in a population of classic and endemic KS. KSHV viremia and various immunologic parameters were screened on 81 patients. KSHV viremia was positive in 58% of the patients. KSHV was detected in B cells, T cells, and monocytes. CD34+ cells depleted in circulating endothelial cells (CECs) were never infected and 50% of the patients tested had CECs infected by KSHV. We observed a significant increase of IL-2 and IFN-gamma production by CD4 T cells and an increase of IFN-gamma production by CD8 T cells compared to control patients. KS progression (P = 0.001) and KS staging (P = 0.03) were significantly and independently associated with positive KSHV viremia. Our results show that there is no specific immunosuppression in classic or endemic KS. We showed that KSHV can be detected within CECs and that KSHV viremia could be an indicator of circulating mature or precursor spindle cells.
authors.affiliation: Laboratory of Pharmacology, INSERM U716, Saint-Louis Hospital, Paris, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Pellet
authors.forename: Delphine
authors.initials: D
authors.lastname: Kerob
authors.forename: Alain
authors.initials: A
authors.lastname: Dupuy
authors.forename: Mary V
authors.initials: MV
authors.lastname: Carmagnat
authors.forename: Samia
authors.initials: S
authors.lastname: Mourah
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Podgorniak
authors.forename: Cecile
authors.initials: C
authors.lastname: Toledano
authors.forename: Patrice
authors.initials: P
authors.lastname: Morel
authors.forename: Olivier
authors.initials: O
authors.lastname: Vérola
authors.forename: Christine
authors.initials: C
authors.lastname: Dosquet
authors.forename: Yamina
authors.initials: Y
authors.lastname: Hamel
authors.forename: Fabien
authors.initials: F
authors.lastname: Calvo
authors.forename: Claire
authors.initials: C
authors.lastname: Rabian
authors.forename: Céleste
authors.initials: C
authors.lastname: Lebbé
mesh_terms: D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D018450:Disease Progression; D042783:Endothelial Cells / Q000821:virology*; D005260:Female; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D012514:Sarcoma, Kaposi / Q000276:immunology / Q000821:virology*; D019562:Viral Load; D014766:Viremia / Q000150:complications*
chemical_list: D007371:Interferon-gamma