title: Effects of prostagladin E(2) on gel-forming mucin secretion in normal human nasal epithelial cells.
abstract: CONCLUSIONS
  The findings of this study indicate that prostaglandin E(2) (PGE(2)) induces MUC5AC gene expression and mucin secretion via the EP4 receptor in cultured normal human nasal epithelial cells.
  
  
  OBJECTIVE
  Recently, PGE(2) was found to induce MUC5AC production via an agonist of EP2/EP4, but not EP1/EP3, in normal human airway epithelium. However, the receptor that mediates MUC5AC has not been determined. This study aimed to investigate the MUC5AC mucin gene and mucin secretion by PGE(2) and its receptors in cultured normal human nasal epithelial cells.
  
  
  METHODS
  After treatment with PGE(2) and/or PGE(2) antagonist, gel-forming mucin mRNA expression was determined by reverse transcription-polymerase chain reaction. Total mucin and MUC5AC mucin levels were measured using an immuno-dot blotting assay.
  
  
  RESULTS
  PGE(2) increased only MUC5AC gene expression and MUC5AC mucin, but not expression of other gel-forming mucin genes. An EP2 receptor antagonist (AH 6809) did not suppress the PGE(2)-induced MUC5AC gene expression or MUC5AC mucin. However, an EP4 receptor antagonist (AH23848) significantly suppressed the level of PGE(2)-induced MUC5AC gene expression and MUC5AC mucin.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Konkuk University College of Medicine, Seoul, Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Kook Kim
authors.forename: Chang
authors.initials: C
authors.lastname: Hoon Kim
authors.forename: Kyubo
authors.initials: K
authors.lastname: Kim
authors.forename: Hyun
authors.initials: H
authors.lastname: Jong Jang
authors.forename: Hyun
authors.initials: H
authors.lastname: Jik Kim
authors.forename: Joo-Heon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D018929:Cell Culture Techniques; D015232:Dinoprostone / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007150:Immunohistochemistry; D055271:Mucin 5AC; D009077:Mucins / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018078:Receptors, Prostaglandin E / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C524678:MUC5AC protein, human; D055271:Mucin 5AC; D009077:Mucins; C545644:PTGER2 protein, human; C545650:PTGER4 protein, human; D012333:RNA, Messenger; D018078:Receptors, Prostaglandin E; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D015232:Dinoprostone