title: In vitro evaluation of Haemonetics MCS+ apheresis platelet concentrates treated with photochemical pathogen inactivation following plasma volume reduction using the INTERCEPT Preparation Set.
abstract: Pathogen inactivation using the INTERCEPT Blood System requires platelet resuspension in InterSol and reduced plasma. Platelets in plasma collected on the Haemonetics MCS+ were processed on the INTERCEPT Preparation Set for plasma volume reduction and addition of InterSol. The use of the Preparation Set resulted in a mean platelet loss of 5.6 +/- 3.4%. Subsequent photochemical treatment (PCT) with amotosalen and ultraviolet A light, and 7 days of storage, resulted in acceptable changes for platelet swirling, lactate, lactate dehydrogenase (LDH), platelet factor-4 (PF4), p-selectin, glycoprotein V (GpV), pO2, pCO2, tumour necrosis factor-alpha (TNF-alpha) and interleukin-8 (IL-8). All platelet units processed with the Preparation Set and PCT met European requirements for leucoreduction and pH values.
authors.affiliation: EFS-Alsace, Strasbourg, France.
authors.forename: H
authors.initials: H
authors.lastname: Isola
authors.forename: D
authors.initials: D
authors.lastname: Kientz
authors.forename: B
authors.initials: B
authors.lastname: Aleil
authors.forename: P
authors.initials: P
authors.lastname: Laeuffer
authors.forename: J
authors.initials: J
authors.lastname: Weil
authors.forename: M-L
authors.initials: ML
authors.lastname: Wiesel
authors.forename: M
authors.initials: M
authors.lastname: Laforêt
authors.forename: L
authors.initials: L
authors.lastname: Lin
authors.forename: V
authors.initials: V
authors.lastname: Mayaudon
authors.forename: J-P
authors.initials: JP
authors.lastname: Cazenave
mesh_terms: D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D017848:Blood-Borne Pathogens / Q000528:radiation effects; D011564:Furocoumarins; D006801:Humans; D066298:In Vitro Techniques; D010777:Photochemistry; D017319:Photosensitizing Agents; D010953:Plasma Volume; D010983:Plateletpheresis / Q000295:instrumentation* / Q000379:methods; D012996:Solutions; D014466:Ultraviolet Rays
chemical_list: D011564:Furocoumarins; D017319:Photosensitizing Agents; D012996:Solutions; C118577:amotosalen