Glutathione-mediated delivery and release using monolayer protected nanoparticle carriers.
We demonstrate here the effective delivery of a dye payload into cells using 2-nm core gold nanoparticles, with release occurring via place exchange of glutathione onto the particle surface. In vitro experiments demonstrate effective release of drug analogues upon addition of glutathione. Cell culture experiments show rapid uptake of nanoparticle and effective release of payload. The role of glutathione in the release process was demonstrated through improved payload release upon transient increase in glutathione levels achieved via introduction of glutathione ethyl ester into the cell.
Hong
Rui
R
Department of Chemistry, University of Massachusetts, Amherst, 01003, USA.
Han
Gang
G
Fernández
Joseph M
JM
Kim
Byoung-jin
BJ
Forbes
Neil S
NS
Rotello
Vincent M
VM
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005978:Glutathione / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006046:Gold; D005978:Glutathione