Effects of proinflammatory cytokines on the expression of mineralization markers and heme oxygenase-1 in human pulp cells.
The roles of IL-1alpha and TNF-alpha in early pulp inflammation were investigated by determining the alkaline phosphatase (ALP) activity, the osteonectin (ON), osteocalcin (OC), bone sialoprotein (BSP), and heme oxygenase-1 (HO-1) expression using an immunoblot method. Primary cultured dental pulp cells were treated with IL-1alpha, TNF-alpha, or both for 3, 7, and 14 days. The pulp cells treated with IL-1alpha for 3 days showed elevated ALP activity and increased ON, OC, and HO-1 expression, whereas TNF-alpha treatment did not increase the ALP activity and no BSP was expressed until day 14. The pulp cells treated with both IL-1alpha and TNF-alpha for 3 days showed increased HO-1 expression compared with that of the control. These data suggest that IL-1alpha and TNF-alpha produced in the early inflammatory reaction have different functions in human pulp cells. IL-1alpha induces ALP, ON, and OC in tooth mineralization and it may play a role in the cytoprotection of pulp cells via HO-1 expression, while long-term treatment of TNF-alpha may inhibit the tooth mineralization.
Min
Kyung-San
KS
Department of Conservative Dentistry, Wonkwang University, Shinyoungdong 344-2, Iksan City, Jeonbu 570-749, South Korea.
Kwon
Young-Yim
YY
Lee
Hwa-Jeong
HJ
Lee
Sun-Kyung
SK
Kang
Kyung-Hwa
KH
Lee
Suk-Keun
SK
Kim
Eun-Cheol
EC
D000469:Alkaline Phosphatase / Q000378:metabolism; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000096:biosynthesis; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology*; D058574:Integrin-Binding Sialoprotein; D007375:Interleukin-1 / Q000494:pharmacology*; D008963:Molar; D015675:Osteocalcin / Q000096:biosynthesis; D015676:Osteonectin / Q000096:biosynthesis; D012795:Sialoglycoproteins / Q000096:biosynthesis; D014074:Tooth Calcification / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
C546871:IBSP protein, human; D018836:Inflammation Mediators; D058574:Integrin-Binding Sialoprotein; D007375:Interleukin-1; D015676:Osteonectin; D012795:Sialoglycoproteins; D014409:Tumor Necrosis Factor-alpha; D015675:Osteocalcin; D051547:Heme Oxygenase-1; D000469:Alkaline Phosphatase