Arachidonic acid induces specific membrane permeability increase in heart mitochondria.
Micromolar concentrations of arachidonic acid cause in Ca2+ loaded heart mitochondria matrix swelling and Ca2+ release. These effects appear to be unrelated to the classical membrane permeability transition (MPT), as they are CsA insensitive, membrane potential independent and can also be activated by Sr2+. Atractyloside potentiated and ATP inhibited the arachidonic acid induced swelling. These observations suggest that the ATP/ADP translocator (ANT) may be involved in the AA induced, CsA insensitive membrane permeability increase. Under the same experimental conditions used for heart mitochondria, arachidonic acid induced the classical CsA sensitive, ADP inhibitable MPT in liver mitochondria.
Di Paola
Marco
M
Institute of Biomembranes and Bioenergetics - Consiglio Nazionale delle Ricerche - Bari, Italy.
Zaccagnino
Patrizia
P
Oliveros-Celis
Constanza
C
Lorusso
Michele
M
D000818:Animals; D016718:Arachidonic Acid / Q000493:pharmacokinetics* / Q000494:pharmacology; D001278:Atractyloside / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D016572:Cyclosporine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008929:Mitochondria, Heart / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism; D000226:Mitochondrial ADP, ATP Translocases / Q000037:antagonists & inhibitors; D051336:Mitochondrial Membranes / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D004791:Enzyme Inhibitors; D001278:Atractyloside; D016718:Arachidonic Acid; D016572:Cyclosporine; D000226:Mitochondrial ADP, ATP Translocases; D002118:Calcium