Synthesis of monodisperse poly(chloromethylstyrene-co-divinylbenzene) beads and their application in separation of biopolymers.
The monodisperse, porous poly(chloromethylstyrene-co-divinylbenzene) beads of 7.9 microm were prepared by a single-step swelling and polymerization method. The seed particles prepared by dispersion polymerization exhibited good absorption of the monomer phase. Based on this media, a weak cation-exchange (WCX) stationary phase for HPLC was synthesized by a new chemically modified method. The prepared resin has advantages of biopolymer separation, high column efficiency, low column backpressure, high protein mass recovery, and good resolution for proteins. The dynamic protein-loading capacity of the synthesized WCX packings was 18.2 mg/g. Five proteins were separated in 3.0 min using the synthesized WCX stationary phase. The experimental results show that the obtained WCX resin has very weak hydrophobicity. The WCX resin was also used for the rapid separation and purification of lysozyme from egg white in 5.0 min with only one step. The purity and specific bioactivity of the purified lysozyme were found to be more than 93% and 70 245 U/mg, respectively.
Gong
Bolin
B
Key Laboratory of Energy and Chemical Engineering, Ningxia University, Yinchuan 750021, P. R. China. gongbl@nxu.edu.cn
Li
Long
L
Zhu
Jinxia
J
Qiang
Kejuan
K
Ren
Li
L
D000818:Animals; D001704:Biopolymers / Q000032:analysis*; D002411:Cation Exchange Resins; D002412:Cations; D002621:Chemistry / Q000379:methods*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004529:Egg White; D008863:Microspheres; D008956:Models, Chemical; D009113:Muramidase / Q000378:metabolism; D011108:Polymers / Q000032:analysis* / Q000737:chemistry; D011137:Polystyrenes / Q000032:analysis* / Q000737:chemistry; D011145:Polyvinyls / Q000032:analysis* / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013997:Time Factors
D001704:Biopolymers; D002411:Cation Exchange Resins; D002412:Cations; D011108:Polymers; D011137:Polystyrenes; D011145:Polyvinyls; D011506:Proteins; C442400:poly(chloromethylstyrene-co-divinylbenzene); D009113:Muramidase