title: [Expression of nestin and neurogenin 3 in the human fetal pancreas].
abstract: OBJECTIVE
  To examine the expression of nestin and neurogenin 3 (Ngn3), the markers of pancreatic stem cells, in the human fetal pancreas.
  
  
  METHODS
  The human fetal pancreas tissue of 12 and 14 weeks were examined for the expression of nestin and Ngn3 using the techniques of immunofluorescence dye and RT-PCR.
  
  
  RESULTS
  Both nestin and Ngn3 expressed widely in 12 and 14 weeks before in human fetal pancreatic tissue. In these positive cells there was no co-expressing insulin or glucagon. There were nestin and Ngn3 co-expressing cells in ducts but not in the islets. The results of RT-PCR also indicated the expression of nestin and Ngn3.
  
  
  CONCLUSIONS
  There was no expression of the markers of mature endocrine cells in the nestin and Ngn3 positive cells, and they were the marks of no-differentiation cells in the human fetal pancreatic tissue.
authors.affiliation: Department of Plastic Surgery, Peking University Third Hospital, Beijing 100083, China.
authors.forename: Zong-mei
authors.initials: ZM
authors.lastname: Zheng
authors.forename: Dong-ming
authors.initials: DM
authors.lastname: Chen
authors.forename: Ling-song
authors.initials: LS
authors.lastname: Li
authors.forename: Jian-ning
authors.initials: JN
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Ai-li
authors.initials: AL
authors.lastname: Lu
authors.forename: Shu-ling
authors.initials: SL
authors.lastname: Wang
authors.forename: Wei-han
authors.initials: WH
authors.lastname: Bao
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis* / Q000235:genetics; D015200:Fluoroimmunoassay; D006801:Humans; D066298:In Vitro Techniques; D007381:Intermediate Filament Proteins / Q000096:biosynthesis* / Q000235:genetics; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D064231:Nestin; D010179:Pancreas / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin; C577484:NES protein, human; C498695:NEUROG3 protein, human