Phenotypic characterization of telomerase-immortalized primary non-malignant and malignant tumor-derived human prostate epithelial cell lines.
In vitro human prostate cell culture models are critical for clarifying the mechanism of prostate cancer progression and for testing preventive and therapeutic agents. Cell lines ideal for the study of human primary prostate tumors would be those derived from spontaneously immortalized tumor cells; unfortunately, explanted primary prostate cells survive only short-term in culture, and rarely immortalize spontaneously. Therefore, we recently have generated five immortal human prostate epithelial cell cultures derived from both the benign and malignant tissues of prostate cancer patients with telomerase, a gene that prevents cellular senescence. Examination of these cell lines for their morphologies and proliferative capacities, their abilities to grow in low serum, to respond to androgen stimulation, to grow above the agar layer, to form tumors in SCID mice, suggests that they may serve as valid, useful tools for the elucidation of early events in prostate tumorigenesis. Furthermore, the chromosome alterations observed in these immortalized cell lines expressing aspects of the malignant phenotypes imply that these cell lines accurately recapitulate the genetic composition of primary tumors. These novel in vitro models may offer unique models for the study of prostate carcinogenesis and also provide the means for testing both chemopreventive and chemotherapeutic agents.
Gu
Yongpeng
Y
Center for Prostate Disease Research, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
Li
Hongzhen
H
Miki
Jun
J
Kim
Kee-Hong
KH
Furusato
Bungo
B
Sesterhenn
Isabell A
IA
Chu
Wei-Sing
WS
McLeod
David G
DG
Srivastava
Shiv
S
Ewing
Charles M
CM
Isaacs
William B
WB
Rhim
Johng S
JS
D000328:Adult; D000368:Aged; D000818:Animals; D002449:Cell Aggregation / Q000502:physiology; D049109:Cell Proliferation; D002877:Chromosomes, Human / Q000235:genetics; D020732:Cytogenetic Analysis; D004268:DNA-Binding Proteins / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000494:pharmacology; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000378:metabolism; D010641:Phenotype; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D019998:Racemases and Epimerases / Q000235:genetics; D011944:Receptors, Androgen / Q000187:drug effects / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
C410798:AR protein, human; D004268:DNA-Binding Proteins; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D019098:Telomerase; D019998:Racemases and Epimerases; C108352:alpha-methylacyl-CoA racemase