[Effects of components isolated from Astragalus mongholicus on expression of p-selectin in shock wave induced kidney injury in rabbit model].
OBJECTIVE
To investigate the effective components of Astragalus mongholicus and their mechanisms in alleviating extracorporeal shock wave lithotripsy (ESWL) induced kidney injury.


METHODS
69 male rabbits were randomly assigned into control group, sham treatment group, Total Saponins of Astragalus (TSA) group, Total Flavonoids of Astragalus (TFA) group, and Total Polysaccharide of Astragalus group (TPA). The shock wave treated kidneys were observed for the expression of p-selectin and the change of cells ultrastructure pre and post ESWL. The concentration of Maleic Dialdehyde (MDA) as well as activity of superoxide dismutase (SOD) in the kidney tissues was also analyzed.


RESULTS
After application of shock wave treatment, p-selectin was expressed extensively in renal glomerulus, renal tubules and renal interstitium of the treated kidneys. It showed a significant increase of MDA levels and decrease of SOD activity in control group (P < 0.05) after ESWL. The comparison between controls and TSA group demonstrated that TSA could significantly reduce the positive rate of p-selectin P < 0.05) and alleviate the injuries of cells ultrastructure. The results also showed a significant decrease of MDA levels and increase of SOD activity in TSA group compared to controls (P < 0.05). The protective effects of TFA and TPA in alleviating kidney injury induced by shock wave were lower than those of TSA; they had no effects of the expression of P-selectin.


CONCLUSIONS
TSA is the main components of A. mongholicus in alleviating shock wave induced kidney injury not only by scavenging oxygen free radicals but also inhibiting the expression of p-selectin.
Li
Xiang
X
Institute of Urology, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi ' an Jiaotong University, Xi ' an 710061, China.
He
Da-lin
DL
Cheng
Xing-fa
XF
Zhang
Lin-lin
LL
Yu
Lin-hua
LH
Li
Jian-jun
JJ
D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007668:Kidney / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology; D007684:Kidney Tubules / Q000378:metabolism / Q000473:pathology; D008096:Lithotripsy / Q000009:adverse effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology; D011817:Rabbits; D011897:Random Allocation; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D005419:Flavonoids; D016166:Free Radical Scavengers; D019007:P-Selectin; D011134:Polysaccharides; D012503:Saponins; D008315:Malondialdehyde; D013482:Superoxide Dismutase