title: [Ultrastructural changes in the cells of human embryo lung fibroblasts in the reproduction of the coronavirus HCoV/SPb/01/03].
abstract: The reproduction of the new coronavirus HCoV/SPb/01/03 in the cultured human embryo lung fibroblasts (HELFBs) was electron microscopically studied. The virus was shown to replicate in the cultured HELFBs, by using for this a cell membrane system and causing profound changes in its morphology. After 24 hours of cell infection, there were mature and defective HCoVISPb/01/03 virions were detected in the vacuoles near the peripheral cisterns of the Golgi apparatus. Some of the vacuoles contained folded membranous structures along with virions.
authors.forename: A P
authors.initials: AP
authors.lastname: Prochukhanova
authors.forename: A K
authors.initials: AK
authors.lastname: Golovanova
authors.forename: A A
authors.initials: AA
authors.lastname: Sominina
authors.forename: T D
authors.initials: TD
authors.lastname: Smirnova
authors.forename: A K
authors.initials: AK
authors.lastname: Sirotkin
authors.forename: V V
authors.initials: VV
authors.lastname: Zarubaev
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure / Q000821:virology; D017934:Coronavirus / Q000502:physiology* / Q000648:ultrastructure; D018352:Coronavirus Infections / Q000821:virology; D005347:Fibroblasts / Q000648:ultrastructure* / Q000821:virology*; D006056:Golgi Apparatus / Q000648:ultrastructure / Q000821:virology; D006801:Humans; D008854:Microscopy, Electron; D014617:Vacuoles / Q000648:ultrastructure / Q000821:virology; D014779:Virus Replication