title: Polymorphic and differential expression of the Trypanosoma cruzi alleles containing universal minicircle binding protein.
abstract: DNA replication mechanisms are poorly understood in most of trypanosomatids, in particular the replication of the peculiar mitochondrial DNA, the kinetoplast DNA (kDNA). To contribute to the knowledge on the mechanism of kDNA replication in Trypanosoma cruzi, we have previously characterized the Universal Minicircle Sequence Binding Protein of this parasite (TcUMSBP), which was first called PDZ5 [E.R. Coelho, T.P. Urmenyi, J. Franco da Silveira, E. Rondinelli, R. Silva, Identification of PDZ5, a candidate universal minicircle sequence binding protein of Trypanosoma cruzi, Int. J. Parasitol. 33 (2003) 853-858]. In this work, we describe two highly polymorphic alleles of the TcUMSBP locus in the T. cruzi reference clone CL Brener and the differential expression pattern of these alleles. A 62 bp sequence in the TcUMSBP upstream intergenic region in one of its alleles affects the efficiency of polycistronic RNA processing and the polyadenylation sites, and therefore regulates the differential expression of TcUMSBP alleles of this locus.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, CCS, Bloco G, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21949-900, Brazil.
authors.forename: Elielton R
authors.initials: ER
authors.lastname: Coelho
authors.forename: Deivid de Carvalho
authors.initials: Dde C
authors.lastname: Rodrigues
authors.forename: Turán P
authors.initials: TP
authors.lastname: Urményi
authors.forename: Edson
authors.initials: E
authors.lastname: Rondinelli
authors.forename: Rosane
authors.initials: R
authors.lastname: Silva
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D003001:Cloning, Molecular; D004261:DNA Replication; D018105:DNA, Kinetoplast / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018274:Electroporation; D005786:Gene Expression Regulation; D016678:Genome; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011110:Polymorphism, Genetic; D011485:Protein Binding; D015800:Protozoan Proteins / Q000096:biosynthesis* / Q000235:genetics*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014162:Transfection; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D018105:DNA, Kinetoplast; D004268:DNA-Binding Proteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; C095676:universal minicircle sequence binding protein, protozoan; D012313:RNA; D015500:Chloramphenicol O-Acetyltransferase