title: Convenient methods for the synthesis of P1-farnesyl-P2-indicator diphosphates.
abstract: To access P1-farnesyl-P2-indicator diphosphates, more efficient methods for the synthesis of farnesyl-phosphate and diphosphates were developed. The procedures reported here provide more flexible conditions than the conventional imidazolide and morpholidate coupling methods. Milder conditions for the synthesis of sensitive allylic diphosphates and greatly improved reaction efficiencies provide access to novel reagents for analysis of diphosphate-based enzymatic reactions.
authors.affiliation: Department of Chemistry, University of California-Irvine, 516 Rowland Hall, Irvine, CA 92697-2025, USA.
authors.forename: Birte K
authors.initials: BK
authors.lastname: Feld
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Weiss
mesh_terms: D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D015394:Molecular Structure; D011106:Polyisoprenyl Phosphates / Q000138:chemical synthesis* / Q000737:chemistry; D012717:Sesquiterpenes
chemical_list: D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; C004808:farnesyl pyrophosphate