title: Protein and genomic organisation of vertebrate MyoR and Capsulin genes and their expression during avian development.
abstract: The related bHLH transcription factors MyoR and Capsulin control craniofacial myogenesis and the development of a number of mesoderm-derived organs in the mouse. However, their molecular function as regulators of differentiation processes is conversely debated. One approach to clarify the roles of these genes is to comparatively analyse their biological and molecular function in various vertebrate models. For this, a prerequisite is the determination of their similarity and their expression patterns. Here we show that vertebrate MyoR and Capsulin are paralogous genes with a high level of conservation regarding their protein sequence, their cDNA sequence and their chromosomal organisation. In the chick, both genes are co-expressed in the developing branchiomeric muscles, the anterior heart field and the splanchnopleura lining the foregut. However, both genes show unique expression domains in trunk skeletal muscle precursors, in the lateral and intermediate mesoderm.
authors.affiliation: Department of Craniofacial Development, King's College London, Floor 27 Guy's Tower, Guy's Hospital, London Bridge, London SE1 9RT, UK.
authors.forename: Gudrun
authors.initials: G
authors.lastname: von Scheven
authors.forename: Ingo
authors.initials: I
authors.lastname: Bothe
authors.forename: Mohi U
authors.initials: MU
authors.lastname: Ahmed
authors.forename: Lúcia E
authors.initials: LE
authors.lastname: Alvares
authors.forename: Susanne
authors.initials: S
authors.lastname: Dietrich
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002642:Chick Embryo; D019295:Computational Biology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D016678:Genome; D017403:In Situ Hybridization; D018006:Myogenic Regulatory Factors / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D018006:Myogenic Regulatory Factors; D011506:Proteins; D014157:Transcription Factors