[Apoptosis and its molecular mechanism in vestibular hair cell after gentamycin toxicity].
OBJECTIVE
To investigate the character of damaged vestibular hair cells caused by gentamycin, and to identify whether apoptosis and phosphorylation of JNK occurs in the damaged cells.


METHODS
Healthy guinea pigs were divided into experimental group and control group randomly with 15 in each group, animals in experimental and control group received systemic injection of gentamycin [100 mg/(kg x d)] and saline respectively for 7 consequent days. All the animals were sacrificed on the 8th day, the crista vestibular of each were observed by preparation and frozen dissection. The apoptosis of hair cell were detected by TUNEL method and phosphorylation of JNK were detected by immunochemistry.


RESULTS
The lesion and apoptosis of hair cells could be seen in the experimental group,and the phosphorylation could be observed too; the hair cells of control group showed no sign of apoptosis or phosphorylation of JNK.


CONCLUSIONS
Gentamycin cause vestibular hair lesion by inducing apoptosis and activation of JNK plays an important role in the procedure of apoptosis.
Jiang
Xuejun
X
Department of Otolaryngology, Affiliated First Hospital of China Medical College, Shenyang, 110001, China. djiangxj@163.com
Li
Wei
W
Zang
Hongrui
H
Wang
Jin
J
Guan
Chao
C
Yang
Ning
N
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D005839:Gentamicins / Q000009:adverse effects*; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D048670:MAP Kinase Kinase 4 / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D014722:Vestibule, Labyrinth / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D005839:Gentamicins; D048670:MAP Kinase Kinase 4