Effect of copper and manganese ions on activities of laccase and peroxidases in three Pleurotus species grown on agricultural wastes.
Copper (Cu2+) and manganese (Mn2+) ions influenced laccase (Lac) and peroxidase production in Pleurotus eryngii, Pleurotus ostreatus, and Pleurotus pulmonarius. In P. eryngii, the optimum Cu2+ concentration for Lac production was 1 mM and for peroxidases 10 mM, and Mn2+ concentration of 5 mM led to peaks of Lac and peroxidase activity. In P. ostreatus HAI 493, the highest level of Lac activity was at Cu2+ concentrations of 1 and 10 mM and Mn2+ concentration of 1 mM, respectively. The absence of Cu2+ and Mn2+ caused the highest levels of peroxidase production. In P. ostreatus HAI 494, the highest level of Lac activity was at a Cu2+ concentration of 5 mM and at Mn2+ concentration of 1 mM, respectively. High levels of peroxidase activity were found in the medium without and with 1 mM Cu2+, and at 1 and 5 mM Mn2+, respectively. In P. pulmonarius, the highest Lac activity was found in the presence of 5 mM Cu2+ and 5 mM Mn2+, respectively. The absence of Cu2+ and Mn2+ as well as their presence at a concentration of 1 mM led to the peaks of peroxidase activities.
Stajic
Mirjana
M
Institute of Botany, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia and Montenegro. stajicm@bfbot.bg.ac.yu
Persky
Limor
L
Hadar
Yitzhak
Y
Friesem
Dana
D
Duletic-Lausevic
Sonja
S
Wasser
Solomon P
SP
Nevo
Eviatar
E
D002384:Catalysis; D002957:Citrus; D003300:Copper / Q000494:pharmacology*; D018556:Crops, Agricultural / Q000378:metabolism / Q000382:microbiology; D005285:Fermentation; D005638:Fruit / Q000378:metabolism / Q000382:microbiology; D042845:Laccase / Q000096:biosynthesis*; D008345:Manganese / Q000494:pharmacology*; D010544:Peroxidases / Q000096:biosynthesis*; D018547:Plant Stems / Q000378:metabolism / Q000382:microbiology; D020076:Pleurotus / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D027843:Vitis
D008345:Manganese; D003300:Copper; D042845:Laccase; D010544:Peroxidases