Human herpesvirus 6 envelope cholesterol is required for virus entry.
In this study, the role of cholesterol in the envelope of human herpesvirus 6 (HHV-6) was examined by using methyl-beta-cyclodextrin (MbetaCD) depletion. When cholesterol was removed from HHV-6 virions with MbetaCD, infectivity was abolished, but it could be rescued by the addition of exogenous cholesterol. HHV-6 binding was affected slightly by MbetaCD treatment. In contrast, envelope cholesterol depletion markedly affected HHV-6 infectivity and HHV-6-induced cell fusion. These results suggest that the cholesterol present in the HHV-6 envelope plays a prominent role in the fusion process and is a key component in viral entry.
Huang
Honglan
H
Department of Microbiology, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Li
Yongmei
Y
Department of Microbiology, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Sadaoka
Tomohiko
T
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
Tang
Huanmin
H
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
Yamamoto
Takahito
T
The Research Foundation for Microbial Diseases of Osaka University, 2-9-41 Yahata-Cho, Kanonji, Kagawa 768-0061, Japan.
Yamanishi
Koichi
K
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.|Department of Microbiology, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Mori
Yasuko
Y
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.|Department of Microbiology, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
D002784:Cholesterol / Q000502:physiology*; D015654:Herpesvirus 6, Human / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism; D014771:Virion / Q000378:metabolism; D047392:beta-Cyclodextrins / Q000494:pharmacology
D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D002784:Cholesterol