title: A bacteroides conjugative transposon, CTnERL, can transfer a portion of itself by conjugation without excising from the chromosome.
abstract: CTnERL, a Bacteroides conjugative transposon, transferred DNA by an Hfr-type mechanism during conjugation when it was excision deficient due to an insertion in the integrase gene. Rescue of the conjugative transposon sequences required the recipient to be RecA proficient and to contain an integrated CTnERL. The transfer efficiency was only 10- to 30-fold lower than the normal element transfer efficiency, and the direction of transfer from the oriT gene showed that the integrase end was transferred first and that the transfer genes were transferred last.
authors.affiliation: Department of Microbiology, 601 S. Goodwin Ave., University of Illinois, Urbana, IL 61801, USA.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Whittle
authors.forename: Nathan
authors.initials: N
authors.lastname: Hamburger
authors.forename: Nadja B
authors.initials: NB
authors.lastname: Shoemaker
authors.forename: Abigail A
authors.initials: AA
authors.lastname: Salyers
mesh_terms: D001439:Bacteroides / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003227:Conjugation, Genetic / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial