[Expression of integrin alpha5 and actin in the cells of intervertebral disc under cyclic hydrostatic pressure in vitro].
OBJECTIVE
To investigate the expression of integrin alpha5 and actin in the cells of intervertebral disc under cyclic hydrostatic pressure in vitro.


METHODS
The porcine lumbar intervertebral disc cells were isolated and cultured in vitro, and the cells underwent cyclic hydrostatic loading. After that, the expression of integrin alpha5 and actin in intervertebral disc cells were studied by means of morphology observing, Western blot and immunohistochemistry staining.


RESULTS
The morphology of intervertebral disc cells were changed into smaller and flatten shape, and the expression of integrin alpha5 and actin were decreased after loading.


CONCLUSIONS
The expression of integrin alpha5 decreases under cyclic hydrostatic pressure, and the actin is affected at the same time when signals are transferred into the cells by integrin alpha5. That may be one of the important mechanisms of the mechanotransduction in the cells of intervertebral disc.
Yu
Sheng-ji
SJ
Department of Orthopaedics, Peking union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Qiu
Gui-xing
GX
Burton
Yang
Y
Sandra
Roth
R
Cari
Whyne
W
Albert
Yee
Y
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D006874:Hydrostatic Pressure / Q000009:adverse effects; D039482:Integrin alpha5 / Q000378:metabolism*; D007403:Intervertebral Disc / Q000166:cytology*; D040542:Mechanotransduction, Cellular; D013552:Swine
D000199:Actins; D039482:Integrin alpha5