Patterns of expression of TSC-22 protein in astrocytic gliomas.
OBJECTIVE
To evaluate expression patterns of protein product of putative tumor suppressor gene TSC-22 in human astrocytic tumors by immunohistochemical approach.


METHODS
Plasmid pET-23d-TSC22 was constructed for the expression of human TSC-22 protein in bacterial system, and polyclonal rabbit antibodies against recombinant TSC-22 were produced. Immunohistochemical analysis of TSC-22 and GFAP expression with the use of anti-human-TSC-22- and anti-human-GFAP-antibodies was performed on histological slides of astrocytic tumors.


RESULTS
Immunohistochemical analysis has shown that the number of cells expressing TSC-22 was significantly lower in glioblastoma tissues than that in diffuse astrocytoma. Double immunohistochemical staining of astrocytic tumors using anti-human-TSC-2- and anti-human-GFAP-antibodies showed that both TSC-22 and GFAP expression is co-localized in astrocytes.


CONCLUSIONS
TSC-22 protein is expressed in astrocytes, but not in macrophage/microglial cells. In more aggressive forms of astrocytic tumors decreased expression of TSC-22 mRNA correlates with its lowered expression on protein level.
Shostak
K O
KO
Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv, Ukraine.
Dmitrenko
V V
VV
Vudmaska
M I
MI
Naidenov
V G
VG
Beletskii
A V
AV
Malisheva
T A
TA
Semenova
V M
VM
Zozulya
Yu P
YP
Demotes-Mainard
J
J
Kavsan
V M
VM
D000595:Amino Acid Sequence; D001253:Astrocytes / Q000378:metabolism; D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D017628:Microglia / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics
D014408:Biomarkers, Tumor; D005904:Glial Fibrillary Acidic Protein; D011994:Recombinant Proteins; D012097:Repressor Proteins; C099726:TSC22D1 protein, human