Flow-injection determination of L-histidine with an immobilized histidine oxidase from Brevibacillus borstelensis KAIT-B-022 and chemiluminescence detection.
A chemiluminometric flow injection analytical system for the quantitation of L-histidine is described. Histidine oxidase (EC 1.4.3.-) from Brevibacillus borstelensis KAIT-B-022 was immobilized on tresylated poly(vinyl alcohol) beads and packed into a stainless-steel column. The hydrogen peroxide produced was detected chemiluminometrically by a flowthrough sensor containing immobilized peroxidase (EC 1.1 1.1.7). The maximum sample throughput was 10 h(-1). The calibration graph was linear from 0.05 to 5 mM; the detection limit (signal to noise ratio = 3) was 0.01 mM. The activity of immobilized histidine oxidase reduced to 65% of the initial value after 350 injections. The system was applied to the determination of L-histidine in fish meat, such as salmon, tunny, bonito, and mackerel.
Kiba
Nobutoshi
N
Department of Applied Chemistry, Faculty of Engineering, University of Yamanashi, Takeda 4-3-11, Kofu 400-8511, Japan.
Koga
Ayumi
A
Tachibana
Masaki
M
Tani
Kazue
K
Koizumi
Hitoshi
H
Koyama
Tetsuo
T
Yamamura
Akira
A
Matsumoto
Kunio
K
Okuda
Tohru
T
Yokotsuka
Koki
K
D001407:Bacillus / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis / Q000379:methods; D006639:Histidine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D010088:Oxidoreductases / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013696:Temperature
D004800:Enzymes, Immobilized; D006639:Histidine; D010088:Oxidoreductases