Intracellular localization and concentration as well as photodynamic effects of benzoporphyrin derivative monoacid ring A in four types of rodent tumor cells.
The relative sensitivities of different tumor cells to photodynamic therapy (PDT) with benzoporphyrin derivative monoacid ring A (BPD-MA) were compared in the four tumor cells. A good correlation was observed between the cell survival at 0.1 microg/ml of BPD-MA and sensitizer uptake/10(6) cells (r = -0.99) or the plating efficiency of cells (r = 0.99). At 3 h after the irradiation, a significant difference was observed in the proportion of apoptotic cells among the four tumor cells (p = 0.024). In conclusion, cell responses to PDT depend on the several factors such as the cell line, photosensitizer dose, and fluence.
Osaki
Tomohiro
T
Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan. ohchan@vetmed.hokudai.ac.jp
Takagi
Satoshi
S
Hoshino
Yuki
Y
Okumura
Masahiro
M
Fujinaga
Toru
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D001692:Biological Transport; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D011166:Porphyrins / Q000493:pharmacokinetics / Q000494:pharmacology*; D011419:Propidium / Q000737:chemistry; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D000077362:Verteporfin
D011166:Porphyrins; D000077362:Verteporfin; D011419:Propidium