title: Calcium calmodulin-stimulated adenylyl cyclases contribute to activation of extracellular signal-regulated kinase in spinal dorsal horn neurons in adult rats and mice.
abstract: The extracellular signal-regulated kinase (Erk) cascades are suggested to contribute to excitatory synaptic plasticity in the CNS, including the spinal cord dorsal horn. However, many of their upstream signaling pathways remain to be investigated. Here, we demonstrate that glutamate and substance P (SP), two principal mediators of sensory information between primary afferent fibers and the spinal cord, activate Erk in dorsal horn neurons of both adult rat and mouse spinal cord. In genetic knock-out mice of calcium calmodulin-stimulated adenylyl cyclase subtypes 1 (AC1) and 8 (AC8), activation of Erk in dorsal horn neurons were significantly reduced or blocked, either after peripheral tissue inflammation or by glutamate or SP in spinal cord slices. Our studies suggest that AC1 and AC8 act upstream from Erk activation in spinal dorsal horn neurons and the calcium-AC1/AC8-dependent Erk signaling pathways may contribute to spinal sensitization, an underlying mechanism for the development of persistent pain after injury.
authors.affiliation: Department of Biomedical Sciences, University of Maryland Dental School, Baltimore, Maryland 21201, USA. fwei@umaryland.edu
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.forename: Kunjumon I
authors.initials: KI
authors.lastname: Vadakkan
authors.forename: Hiroki
authors.initials: H
authors.lastname: Toyoda
authors.forename: Long-Jun
authors.initials: LJ
authors.lastname: Wu
authors.forename: Ming-Gao
authors.initials: MG
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Fanny W F
authors.initials: FW
authors.lastname: Shum
authors.forename: Yong Heng
authors.initials: YH
authors.lastname: Jia
authors.forename: Min
authors.initials: M
authors.lastname: Zhuo
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism* / Q000502:physiology; D000367:Age Factors; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006358:Hot Temperature; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020671:Posterior Horn Cells / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004791:Enzyme Inhibitors; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; D000262:Adenylyl Cyclases