[Immortalized human odontoblast-like cell line expressed dentin extracellular matrix in vitro].
OBJECTIVE
To determine whether dentin matrix proteins were expressed by the human odontoblast-like cell line hTERT-hOd-1 in vitro.


METHODS
Collagen type I, bone sialoprotein (BSP), dentin matrix protein 1 (DMP1) and the marker for odontoblast, dentin sialophosphoprotein (DSPP) and dentin sialoprotein (DSP) were detected in these cells by immunohistochemistry, RT-PCR and in situ hybridization. During being cultured in mineralizing medium for 5 weeks, the secretion of OC and activity of ALP were measured once a week.


RESULTS
DSPP, DMP1, BSP and collagen type I were expressed in hTERT-hOd-1 either at mRNA or protein level. Under the induction of mineralizing medium, the cells showed higher activity of ALP and increased secretion of OC.


CONCLUSIONS
hTERT-hOd-1 expressed dentin extracellular matrix in vitro, which means the cell line has the potential of mineralization.
Wang
Han-guo
HG
Dept of Operative Dentistry and Endodontics, College of Stomatology, The Fourth Military Medical University, Xi'an, China.
Xiao
Ming-zhen
MZ
Zhao
Shou-liang
SL
Hao
Jian-jun
JJ
Zhang
Min
M
D002460:Cell Line; D024042:Collagen Type I; D003804:Dentin; D005109:Extracellular Matrix; D016326:Extracellular Matrix Proteins; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D009804:Odontoblasts; D010750:Phosphoproteins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein
D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein