Organic solvent-selective domain of the resistance-nodulation-division-type xenobiotic-antibiotic transporters of Pseudomonas aeruginosa.
The hydrophobic substrate-selective domain of the resistance-nodulation-division-type xenobiotic transporter of Pseudomonas aeruginosa was assigned based on the different organic-solvent selectivities of MexB and MexY. The MexB-MexY hybrid protein consisting of two large periplasmic domains of MexB and the transmembrane domains of MexY showed MexB-type organic solvent selectivity. The results imply that the resistance-nodulation-division-type xenobiotic transporters recognize hydrophobic substrates such as organic solvents by their periplasmic domains and expel them to the external milieu. This is an elegant way to protect the cytoplasmic membrane from membrane-deteriorating agents.
Li
Xiaohong
X
Institute of Medical Science, Tokai University, Isehara, Kanagawa 259-1193, Japan.
Eda
Shima
S
Nakae
Taiji
T
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000502:physiology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000502:physiology*; D001692:Biological Transport; D024881:Drug Resistance, Bacterial; D057927:Hydrophobic and Hydrophilic Interactions; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000502:physiology*; D009930:Organic Chemicals / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000502:physiology*; D012997:Solvents / Q000378:metabolism; D013379:Substrate Specificity; D015262:Xenobiotics / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; C118597:MexB protein, Pseudomonas aeruginosa; C402791:MexXY protein, Pseudomonas aeruginosa; D009930:Organic Chemicals; D012997:Solvents; D015262:Xenobiotics