title: Identification of immunodominant excretory-secretory cysteine proteases of adult Paragonimus westermani by proteome analysis.
abstract: Paragonimus westermani causes inflammatory lung disease in humans. The parasite excretes a host of biologically active molecules, which are thought to be involved in pathophysiological and immunological events during infection. Analyses of the 2-DE protein profiles of the excretory-secretory products (ESP) of adult P. westermani revealed approximately 147 protein spots, at least 15 of which were identified as cysteine proteases (CPs), at pHs between 4.5 and 8.5, and molecular weights (MWs) between 27 and 35 kDa. An additional three CPs (designated as PwCP-3, -8 and -11) were newly recognized by TOF/TOF MS. Their molecular biological information, which shared a high level sequence homology, was elucidated. The majority of the CPs reacted strongly with sera from paragonimiasis patients. When we observed the chronological changes in the antibody responses of the respective CPs against canine sera collected serially at 1, 3, 5, 7, 11 and 14 wk after experimental infection, these molecules exhibited a multiplicity of distinct immune recognition patterns. Our results clearly showed that P. westermani adult ESP were principally composed of excretory-secretory CPs, and that these CPs may exert effects not only on host tissue degradation and nutrient uptake, but also on the immune-regulating cells via synergistic and independent interactions.
authors.affiliation: Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon, Korea.
authors.forename: Eung-Goo
authors.initials: EG
authors.lastname: Lee
authors.forename: Byoung-Kuk
authors.initials: BK
authors.lastname: Na
authors.forename: Young-An
authors.initials: YA
authors.lastname: Bae
authors.forename: Seon-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Je
authors.forename: Jeong-Won
authors.initials: JW
authors.lastname: Ju
authors.forename: Shin-Hyeong
authors.initials: SH
authors.lastname: Cho
authors.forename: Tong-Soo
authors.initials: TS
authors.lastname: Kim
authors.forename: Shin-Yong
authors.initials: SY
authors.lastname: Kang
authors.forename: Seung-Yull
authors.initials: SY
authors.lastname: Cho
authors.forename: Yoon
authors.initials: Y
authors.lastname: Kong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000276:immunology / Q000378:metabolism; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional; D015801:Helminth Proteins / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010237:Paragonimiasis / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism; D048492:Paragonimus westermani / Q000201:enzymology* / Q000254:growth & development / Q000276:immunology; D020543:Proteome / Q000032:analysis*; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D015801:Helminth Proteins; D020543:Proteome; D003546:Cysteine Endopeptidases