title: HPLC quantitation of the four stereoisomers of benzoxathiepin derivatives with cellulose phenyl type chiral stationary phase and circular dichroism detection.
abstract: The chiral separation of a new antianginal agent has been investigated on a chiral cellulose column with UV and circular dichroism (CD) detection. This benzoxathiepin derivative under development has two stereogenic centers whose (R,S) stereoisomer shows an interesting antianginal activity. After optimisation of the mobile phase composition, a baseline-resolved separation of the four stereoisomers was achieved on a Chiralcel OJ-H chiral column by using methanol-ethanol-diethylamine (25:75:0.1, v/v/v) as mobile phase. The CD detection system allowed quantitation and a linear response was observed within a 10-200 microg mL-1 concentration range (r2=0.9966) and limit of quantification down to 2 microg mL-1 was achieved.
authors.affiliation: Institut de Chimie Organique et Analytique (ICOA), CNRS FR 2708, UMR 6005, Université d'Orléans, BP 6759, 45067 Orléans cedex 2, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Beaufour
authors.forename: Philippe
authors.initials: P
authors.lastname: Morin
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Ribet
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Maurizot
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002942:Circular Dichroism; D015203:Reproducibility of Results; D012702:Serotonin Antagonists / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013846:Thiepins / Q000032:analysis* / Q000737:chemistry
chemical_list: D012702:Serotonin Antagonists; D013846:Thiepins; C070231:Chiracel OJ; D002482:Cellulose