Lab assembly of a low-cost, robust SYBR green buffer system for quantitative real-time polymerase chain reaction.
Pellissier
François
F
Department of Biochemistry, University of Geneva, 30 Quai Ernest Ansermet, Sciences II, 1211 Geneva 4, Switzerland.
Glogowski
Carolina M
CM
Heinemann
Stephen F
SF
Ballivet
Marc
M
Ossipow
Vincent
V
D052160:Benzothiazoles; D002021:Buffers; D003959:Diamines; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000191:economics; D005559:Formamides; D009930:Organic Chemicals / Q000737:chemistry* / Q000191:economics; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D011804:Quinolines; D011933:Reagent Kits, Diagnostic / Q000191:economics; D012680:Sensitivity and Specificity; D014199:Trehalose
D052160:Benzothiazoles; D002021:Buffers; D003959:Diamines; D005456:Fluorescent Dyes; D005559:Formamides; D009930:Organic Chemicals; D011804:Quinolines; D011933:Reagent Kits, Diagnostic; C098022:SYBR Green I; C031066:formamide; D014199:Trehalose