title: [Genetic polymorphism of Malassezia spp. yeast isolates from individuals with and without dermatological lesions].
abstract: BACKGROUND
  Malassezia spp. yeasts are opportunistic and newly emergent diseases. One or more species have been isolated in association with dermatological pathology and systemic disease. Their pathological role has not been fully elucidated since Malassezia spp. are common in normal skin flora.
  
  
  OBJECTIVE
  In the current study, a search was undertaken for genetic markers in the Malassezia spp. isolates that correlate with each type of dermatologic lesion.
  
  
  METHODS
  A total of 103 strains of Malassezia spp were isolated from patients with pytiriasis versicolor, seborrheic dermatitis, seborrheic dermatitis HIV (+), and atopic dermatitis, as well as from healthy individuals. Eight isolates from CBS Centraalbureau voor Schimmelcultures (Netherlands) were used as controls. Fingerprinting was done using random amplification of polymorphic DNA technique (RAPD) with three primers (OPA2, OPA4, OPA13). The data were analyzed with Diversity Database and SYN-TAX-PC programs.
  
  
  RESULTS
  Intraspecies genetic heterogeneity in M. furfur, M. globosa, M. restricta, M. slooffiae, M. obtusa was observed, whereas M. sympodialis showed the greatest homogeneity.
  
  
  CONCLUSIONS
  The dermatological disease caused by these different species was not associated with distinctive RAPD fingerprints.
authors.affiliation: Centro de Investigaciones Microbiológicas, Universidad de los Andes, Bogota, DC, Colombia.
authors.forename: Adriana M
authors.initials: AM
authors.lastname: Celis
authors.forename: María Caridad
authors.initials: MC
authors.lastname: Cepero de García
mesh_terms: D016022:Case-Control Studies; D004271:DNA, Fungal / Q000032:analysis; D006801:Humans; D008290:Malassezia / Q000235:genetics*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D014010:Tinea Versicolor / Q000209:etiology
chemical_list: D004271:DNA, Fungal