The determination of nicotinic acid in plasma by mixed-mode liquid chromatography-tandem mass spectrometry following ion exchange solid phase extraction.
An assay for nicotinic acid in plasma samples has been developed using ion exchange solid phase extraction in 96-well format followed by mixed-mode ion exchange/reversed-phase liquid chromatography with positive ion tandem mass spectrometry detection. The assay avoids the need for time-consuming derivatisation procedures or involatile ion-pair chromatography reagents. The assay is linear over the wide range 0.05-20 microg/mL, based on a 100 microL sample (correlation coefficient>0.99). The assay is accurate and precise (bias and coefficient of variation<18%) over this calibration range.
Mallett
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DN
Drug Metabolism and Pharmacokinetics, Cardiovascular and Urogenital Centre of Excellence in Drug Discovery, GlaxoSmithKline R&D, Gunnels Wood Road, Stevenage, Herts, UK. dave.n.mallett@gsk.com
Dayal
S
S
Dear
G J
GJ
Pateman
A J
AJ
D000284:Administration, Oral; D000818:Animals; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods; D013058:Mass Spectrometry; D009525:Niacin / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results
D009525:Niacin