title: Stability of the BK polyomavirus genome in renal-transplant patients without nephropathy.
abstract: To clarify the stability of the BK polyomavirus (BKPyV) genome in renal transplant (RT) recipients, three to five complete BKPyV genomes from each of six RT recipients with surviving renal allografts were molecularly cloned. The complete sequences of these clones were determined and compared in each patient. No nucleotide difference was detected among clones in two patients, and a few nucleotide variations were found among those in four patients. In each of these patients a parental sequence (usually the major sequence), from which variant sequences (usually minor sequences) with nucleotide substitutions would have been generated, were identified. A comparison between the parental and variant sequences in each patient identified a single nucleotide substitution in each variant sequence. From these findings, it was concluded that the genome of BKPyV is stable in RT recipients without nephropathy, with only minor nucleotide substitutions in the coding region.
authors.affiliation: Department of Urology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Takasaka
authors.affiliation: Department of Urology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Goya
authors.affiliation: Department of Urology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Ishida
authors.affiliation: Department of Urology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Tanabe
authors.affiliation: Department of Urology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Toma
authors.affiliation: Department of Urology, Iwate Medical University School of Medicine, Morioka, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Fujioka
authors.affiliation: Department of Urology, Iwate Medical University School of Medicine, Morioka, Japan.
authors.forename: So
authors.initials: S
authors.lastname: Omori
authors.affiliation: Japanese Foundation for AIDS Prevention, Tokyo, Japan.|Department of Urology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Huai-Ying
authors.initials: HY
authors.lastname: Zheng
authors.affiliation: Department of Urology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Kobe Institute of Health, Kobe, Japan.
authors.forename: Souichi
authors.initials: S
authors.lastname: Nukuzuma
authors.affiliation: Department of Urology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Tadaichi
authors.initials: T
authors.lastname: Kitamura
authors.affiliation: Department of Urology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yogo
mesh_terms: D001739:BK Virus / Q000235:genetics* / Q000502:physiology; D036022:Capsid Proteins / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000150:complications; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D007674:Kidney Diseases / Q000821:virology; D016030:Kidney Transplantation; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D036022:Capsid Proteins; C062481:VP1 protein, polyomavirus