Detection of TLS/FUS-CHOP fusion transcripts in a case of oral liposarcoma.
OBJECTIVE
The aim of this study was to detect the chromosomal translocation t(12;16)(q13;p11) that leads to a gene fusion encoding a FUS-CHOP chimeric protein and has been shown to be highly characteristic of myxoid and round cell subtypes of liposarcoma, in a case of oral myxoid liposarcoma.


METHODS
Nested reverse transcriptase-polymerase chain reaction to detect the TLS/FUS-CHOP fusion gene transcript was performed. A case of inflammatory fibrous hyperplasia and a case of oral lipoma were included as negative controls.


RESULTS
Only the myxoid oral liposarcoma showed a 103-base pair product, specific of TLS/FUS-CHOP fusion type II transcript.


CONCLUSIONS
The identification of FUS-CHOP transcript is potentially useful in the diagnosis and research of oral liposarcomas.
Rivero
Elena Riet Correa
ER
Department of Oral Pathology, School of Dentistry, University of São Paulo, 05508-900 São Paulo, Brazil.
Mesquita
Ricardo Alves
RA
de Sousa
Suzana Cantanhede Orsini Machado
SC
Nunes
Fabio Daumas
FD
D002881:Chromosomes, Human, Pair 12; D002885:Chromosomes, Human, Pair 16; D006801:Humans; D008080:Liposarcoma / Q000235:genetics* / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis; D034702:RNA-Binding Protein FUS / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012983:Soft Tissue Neoplasms / Q000235:genetics* / Q000473:pathology; D051742:Transcription Factor CHOP / Q000235:genetics* / Q000378:metabolism; D014178:Translocation, Genetic
D015514:Oncogene Proteins, Fusion; D012334:RNA, Neoplasm; D034702:RNA-Binding Protein FUS; C106030:TLS-CHOP fusion protein, human; D051742:Transcription Factor CHOP