Electroporation as a tool to transfer the plasmid pRL489 in Oscillatoria MKU 277.
To establish the gene transfer system in the cyanobacteria, we successfully introduced the shuttle plasmid pRL489 into Oscillatoria MKU 277 by electroporation with transformation frequencies of up to 102 cfu/mug of plasmid DNA. These findings suggest that Oscillatoria MKU 277 can be used as an experimental tool for genetic maneuvering.
Ravindran
C R Marutha
CR
Department of Pharmacology, M.C. 7764, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA. ravindran@uthscsa.edu
Suguna
S
S
Shanmugasundaram
S
S
D000458:Cyanobacteria / Q000235:genetics*; D018274:Electroporation; D018014:Gene Transfer Techniques; D010957:Plasmids / Q000235:genetics*; D014169:Transformation, Bacterial