Characterisation of potentially probiotic vaginal lactobacilli isolated from Argentinean women.
This study aims to evaluate potentially beneficial properties of 20 strains of vaginal lactobacilli isolated from women in Tucumán, Argentina, by determining acid and hydrogen peroxide production and auto-aggregation ability. The microorganisms were characterised genetically by amplified ribosomal 16S-DNA restriction analysis (ARDRA). Lactobacillus gasseri and L. rhamnosus were the predominant species identified among the 20 vaginal lactobacilli strains. Most achieved low pH values after 12 h incubation at 37 degrees C and produced hydrogen peroxide in static culture. However, pH decrease and semi-quantitative hydrogen peroxide production of most homofermentative lactobacilli were significantly higher than those of heterofermentative lactobacilli. Of the 20 strains studied, only three demonstrated remarkable auto-aggregation patterns. Four strains were selected for possible use in a probiotic product for vaginal application; however, further in vitro study of other potentially probiotic characteristics is required before attempting clinical trials.
Juárez Tomás
M S
MS
Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco, Tucumán, Argentina.
Zonenschain
D
D
Morelli
L
L
Nader-Macías
M E
ME
D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014621:Vagina / Q000382:microbiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D006861:Hydrogen Peroxide