Standardization of a novel blood-sampling method through the jugular vein for use in the quantified [14C] 2-deoxyglucose method.
In the traditional [14C] deoxyglucose (2DG) method for the measurement of local cerebral glucose utilization (LCGU), blood samples are collected from the femoral artery. However, the placement of a femoral catheter can affect locomotor activity of the animal. We wanted to develop a new technique for blood sampling that would not interfere with the ongoing behavior. Therefore, the present report establishes a method of collecting blood samples for the 2DG method through the jugular vein. To calibrate this method, catheters were inserted in both the femoral artery and jugular vein of adult male Sprague Dawley rats. The next day, rats were injected with 2DG (125 microCi/kg) through the jugular vein. To quantify 14C in plasma, the standard method of blood collection was used for the femoral artery while syringes were used to extract blood samples from the jugular vein. We calculated the integrated specific activity of the plasma and final tissue 2DG concentrations based on Sokoloff's original equation using blood samples derived from both vessels. LCGU determined in selected brain regions was equivalent using both sampling methods. In conclusion, sampling from the jugular vein is appropriate for the quantified 2DG method and does not disrupt locomotor activity of the rat.
Torres-Reverón
Annelyn
A
Program in Neural and Behavioral Sciences, 450 Clarkson Ave, Box 29, Brooklyn, NY 11209, USA.
Melnick
Susan M
SM
Stephenson
Stacy I
SI
Dow-Edwards
Diana L
DL
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002247:Carbon Isotopes; D002408:Catheters, Indwelling; D003847:Deoxyglucose / Q000097:blood / Q000378:metabolism*; D005263:Femoral Artery; D006403:Hematologic Tests / Q000379:methods*; D007601:Jugular Veins; D008297:Male; D009043:Motor Activity; D051381:Rats; D017207:Rats, Sprague-Dawley
D002247:Carbon Isotopes; D003847:Deoxyglucose