A distinct type of cyclin D, CYCD4;2, involved in the activation of cell division in Arabidopsis.
The Arabidopsis genome encodes 10 D-type cyclins (CYCD); however, their differential role in cell cycle control is not well known. Among them, CYCD4;2 is unique in the amino acid sequence; namely, it lacks the Rb-binding motif and the PEST sequence that are conserved in CYCDs. Here, we have shown that CYCD4;2 suppressed G1 cyclin mutations in yeast and formed a kinase complex with CDKA;1, an ortholog of yeast Cdc28, in insect cells. Hypocotyl explants of CYCD4;2 over-expressing plants showed faster induction of calli than wild-type explants on a medium containing lower concentration of auxin. These results suggest that CYCD4;2 has a promotive function in cell division by interacting with CDKA;1 regardless of the unusual primary sequence.
Kono
Atsushi
A
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Tokyo 113-0032, Japan.
Ohno
Ryoko
R
Umeda-Hara
Chikage
C
Uchimiya
Hirofumi
H
Umeda
Masaaki
M
D000818:Animals; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D017489:CDC28 Protein Kinase, S cerevisiae / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D056741:Cyclin D; D018844:Cyclin-Dependent Kinases / Q000502:physiology; D016213:Cyclins / Q000235:genetics / Q000502:physiology*; D018546:Hypocotyl / Q000235:genetics / Q000502:physiology; D007313:Insecta / Q000166:cytology; D011485:Protein Binding; D015003:Yeasts / Q000235:genetics / Q000502:physiology
D029681:Arabidopsis Proteins; D056741:Cyclin D; D016213:Cyclins; D017489:CDC28 Protein Kinase, S cerevisiae; C434472:CDKA1 protein, Arabidopsis; D018844:Cyclin-Dependent Kinases