Effect of landomycin E on expression of mRNA coding for transforming growth factor beta ligands and specific receptors in MCF-7 cells.
The aim of the present study was to investigate regulation of mRNA, coding for TGFbeta ligands and specific receptors, by novel antitumor antibiotic landomycin E (LE) in human breast adenocarcinoma cells of MCF-7 line, sensitive and resistant to anti-cancer agent doxorubicin.


METHODS
Semi-quantitative analysis of mRNA expression was estimated using RT-PCR and DNA gel-electrophoresis methods. For comparison, another anti-cancer drug doxorubicin (adriamycin) was utilized. THE RESULTS obtained indicate that LE induces up-regulation of mRNA coding for TGFbeta receptors type I and type II in MCF-7 cells, as well as in their doxorubicin-resistant sub-line.


CONCLUSIONS
Anti-tumor action of LE may be mediated by TGFbeta, and a signaling pathway of this cytokine is not affected by the development of tumor cell resistance to doxorubicin.
Panchuk
R
R
Institute of Cell Biology, NAS of Ukraine, Lviv 79005, Ukraine.
Korynevska
A
A
Stoika
R
R
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism*; D000617:Aminoglycosides / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008024:Ligands; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects; D018125:Receptors, Transforming Growth Factor beta / Q000187:drug effects / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000187:drug effects / Q000235:genetics*
D000617:Aminoglycosides; D000970:Antineoplastic Agents; D008024:Ligands; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; C113593:landomycin E; D004317:Doxorubicin