An immunocytometric assay based on dengue infection via DC-SIGN permits rapid measurement of anti-dengue neutralizing antibodies.
Dengue remains a global public health threat and development of a safe and effective vaccine is a principal public health goal. The primary correlate of immunity is thought to be neutralizing antibodies. Currently, the plaque reduction neutralization test (PRNT) is the gold standard measure of dengue neutralizing antibody responses, but this test is limited by time-consuming performance. In addition, some feel that use of viral strains adapted to grow in Vero or BHK cells may not accurately reflect protective responses. A human cell line transfected to express a putative natural dengue receptor, DC-SIGN (CD209), was used to measure antibody-mediated dengue neutralization. Using neutralizing monoclonal antibodies, immune sera, and laboratory adapted dengue viruses, serotype-specific neutralizing activity was demonstrated similar to that seen in the Vero PRNT. Importantly, serotype-specific neutralizing activity against recently isolated dengue strains with less heterotypic cross-neutralization than laboratory adapted viruses was also demonstrated.
Martin
Nicole C
NC
Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
Pardo
Jorge
J
Simmons
Monika
M
Tjaden
Jeffrey A
JA
Widjaja
Susana
S
Marovich
Mary A
MA
Sun
Wellington
W
Porter
Kevin R
KR
Burgess
Timothy H
TH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D015815:Cell Adhesion Molecules / Q000378:metabolism; D045744:Cell Line, Tumor / Q000378:metabolism; D003715:Dengue / Q000097:blood*; D003716:Dengue Virus / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism; D051379:Mice; D009500:Neutralization Tests / Q000379:methods*; D011956:Receptors, Cell Surface / Q000378:metabolism; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin