title: Isolation of Helicobacter pylori from the intestinal mucosa of patients with Crohn's disease.
abstract: BACKGROUND
  Helicobacter species are associated with inflammatory bowel disease in rodents and in nonhuman primates. Therefore, we prospectively investigated the presence of Helicobacter species in the intestinal mucosa of patients with and without Crohn's disease by culture and polymerase chain reaction (PCR) assays.
  
  
  METHODS
  Mucosal fragments were obtained from the ileum, different colon regions, and rectum of 43 patients with Crohn's disease and of 74 patients without inflammatory bowel disease.
  
  
  RESULTS
  Helicobacter pylori strains, identified by 16S rRNA gene sequencing, were more frequently isolated and PCR-detected in the intestinal mucosa of patients with ulcerative colitis-like Crohn's disease than in intestinal mucosa of the control group. Otherwise, anti-H. pylori immunoglobulin G levels were significantly lower in fibrostenosing and fistulating Crohn's disease subgroups. No other Helicobacter species were found in the intestinal mucosa of the patients.
  
  
  CONCLUSIONS
  Although our results suggest an association between the presence of H. pylori in the intestine and ulcerative colitis-like phenotype of Crohn's disease, H. pylori infection in the actual causality of Crohn's disease is still to be determined.
authors.affiliation: Laboratory of Research in Bacteriology and Department of Microbiology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Adriana Gonçalves
authors.initials: AG
authors.lastname: Oliveira
authors.forename: Gifone Aguiar
authors.initials: GA
authors.lastname: Rocha
authors.forename: Andreia Maria Camargos
authors.initials: AM
authors.lastname: Rocha
authors.forename: Maria das Graças Pimenta
authors.initials: Md
authors.lastname: Sanna
authors.forename: Sílvia Beleza
authors.initials: SB
authors.lastname: Moura
authors.forename: Renato
authors.initials: R
authors.lastname: Dani
authors.forename: Frederico Passos
authors.initials: FP
authors.lastname: Marinho
authors.forename: Liano Sia
authors.initials: LS
authors.lastname: Moreira
authors.forename: Maria de Lourdes Abreu
authors.initials: Mde L
authors.lastname: Ferrari
authors.forename: Lúcia Porto Fonseca
authors.initials: LP
authors.lastname: Castro
authors.forename: Dulciene Maria Magalhães
authors.initials: DM
authors.lastname: Queiroz
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003424:Crohn Disease / Q000209:etiology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007413:Intestinal Mucosa / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D007074:Immunoglobulin G; D012336:RNA, Ribosomal, 16S