title: Preparation and characterization of recombinant golgin tethers.
abstract: Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: Ayano
authors.initials: A
authors.lastname: Satoh
authors.forename: Matthew
authors.initials: M
authors.lastname: Beard
authors.forename: Graham
authors.initials: G
authors.lastname: Warren
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000302:isolation & purification*; D003598:Cytoskeletal Proteins / Q000302:isolation & purification*; D004268:DNA-Binding Proteins; D006056:Golgi Apparatus / Q000378:metabolism*; D000077614:Golgi Matrix Proteins; D020662:Guanine Nucleotide Exchange Factors / Q000302:isolation & purification*; D008565:Membrane Proteins / Q000302:isolation & purification*; D017434:Protein Structure, Tertiary; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification; D064067:Rho Guanine Nucleotide Exchange Factors; D014157:Transcription Factors; D033921:Vesicular Transport Proteins
chemical_list: D001324:Autoantigens; C088424:Cux1 protein, rat; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; C060042:GOLGA5 protein, human; D000077614:Golgi Matrix Proteins; C097741:Golgin subfamily A member 2; D020662:Guanine Nucleotide Exchange Factors; D008565:Membrane Proteins; D011994:Recombinant Proteins; D064067:Rho Guanine Nucleotide Exchange Factors; D014157:Transcription Factors; D033921:Vesicular Transport Proteins; C087574:macrogolgin