Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms.
The genetic variability of 61 Trypanosoma cruzi isolates from 47 chronic chagasic patients of Minas Gerais state was analyzed by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) using M13-40, lambdagt11-F, and L15996 primers. Cluster analysis by unweighted pair group method analysis was applied to RAPD profiles, and cluster analysis used to verify a possible correlation among different clinical forms of the disease from these patients. The T. cruzi isolates showed distinct grouping on tree topology, with the isolates not being possible to establish a correlation to the clinical forms of Chagas' disease. These data showed that the T. cruzi isolates from these patients would compose a group of populations well correlated genetically.
D'Avila
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DA
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 Caixa Postal 486, 31270-901, Belo Horizonte, Minas Gerais, Brazil. galvao@icb.ufmg.br
Gontijo
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ED
Lages-Silva
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E
Meira
W S F
WS
Chiari
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E
Galvão
L M C
LM
D000328:Adult; D000818:Animals; D001938:Brazil; D014355:Chagas Disease / Q000469:parasitology* / Q000503:physiopathology*; D002648:Child; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D016054:DNA, Protozoan