title: [Effects of electroacupuncture treatment on nitrergic neurotransmitter in bladder neck and detrusor of rats with unstable bladder].
abstract: OBJECTIVE
  To observe the effects of electroacupuncture treatment on nitrergic neurotransmitter in bladder neck and detrusor of rats with unstable bladder.
  
  
  METHODS
  Rat models of unstable bladder were established by operation to induce urethral obstruction. Electroacupuncture treatment was given by acupuncturing Huiyang and Zhonglushu points for a week. Then the neuronal nitric oxide synthase (nNOS)-, endothelial nitric oxide synthase (eNOS)- and inducible nitric oxide synthase (iNOS)-positive cells in bladder neck and detrusor of the rats were observed.
  
  
  RESULTS
  The nitrergic neurotransmitter in bladder neck and detrusor were obviously decreased in rats with unstable bladder. The electroacupuncture treatment could significantly increase the contains of NOS in bladder tissue.
  
  
  CONCLUSIONS
  To promote the synthesis and secretion of nitrergic neurotransmitter in bladder tissue may be one of the mechanisms of acupuncture in adjusting bladder function.
authors.affiliation: Department of Acupuncture and Moxibustion, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China. chenyuelai@163.com
authors.forename: Yue-Lai
authors.initials: YL
authors.lastname: Chen
authors.forename: Jue
authors.initials: J
authors.lastname: Cen
authors.forename: Wen-Guang
authors.initials: WG
authors.lastname: Hou
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Gao
authors.forename: Xian-Min
authors.initials: XM
authors.lastname: Yu
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Ma
mesh_terms: D000818:Animals; D015671:Electroacupuncture; D008297:Male; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D001750:Urinary Bladder, Neurogenic / Q000201:enzymology / Q000503:physiopathology / Q000628:therapy*; D014549:Urinary Incontinence / Q000201:enzymology / Q000503:physiopathology / Q000628:therapy*; D014554:Urination / Q000502:physiology*
chemical_list: D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III