[Effect of vitamin C antioxidative protection on human red blood cells].
In order to investigate the effect of antioxidants on human blood, vitamin C was selected and added into plastic blood storage bags with CPD, and stored at 25 degrees C. During 6 days of storage, some indexes as ATP, SOD, MDA, K(+) concentration and superoxide radicals were detected and were compared with control group, The results showed that ATP and SOD activity in whole blood with vitamin C during 6 days of storage was higher then that in control group (P < 0.05, P < 0.01), the MDA and plasma K(+) concentrations in stored whole blood with vitamin C during 6 days of storage were lower than that in control group (P < 0.05, P < 0.01), the superoxide radical concentrations in stored whole blood with vitamin C decreased lower than that in control group (30%). The conclusion was made that vitamin C increases activities of ATP and SOD, decreases concentrations of MDA, plasma K(+) and superoxide radicals during blood preservation.
Zan
Tong
T
Blood Center of Jiangsu Province, Nanjing 210042, China.
Tao
Jing
J
Tang
Rong-Cai
RC
Liu
Yan-Chun
YC
Liu
Yi
Y
Huang
Bin
B
Zhou
Jing-Yu
JY
Wu
Min-Hui
MH
Liu
Hong-Li
HL
D000255:Adenosine Triphosphate / Q000097:blood; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D001793:Blood Preservation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008315:Malondialdehyde / Q000097:blood; D011188:Potassium / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors
D000975:Antioxidants; D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D013482:Superoxide Dismutase; D001205:Ascorbic Acid; D011188:Potassium