title: Difference in adhesion molecule expression (ICAM-1 and VCAM-1) in juvenile and adult dermatomyositis, polymyositis and inclusion body myositis.
abstract: To assess the differential expression of adhesion molecules ICAM-1 and VCAM-1 in vessels and muscle fibers in acquired inflammatory myopathy, a series comprising thirty-seven muscle biopsy specimens from patients with JDM, fifteen with DM, fifteen with PM and seven with IBM was studied. Histochemical and immunohistochemical tests (StreptABCcomplex/HRP) for ICAM-1 and VCAM-1 (Dakopatts) were performed in serial frozen sections. ICAM-1 expression in vessels was significantly (p<0.0001) more present in JDM than PM, DM or IBM. However, in muscle fibers, ICAM-1 expression was absent in both JDM and IBM, but present in 33.4% and 40% in PM and DM respectively (p<0.0001). VCAM-1 expression in vessels was significantly more present in PM and DM than JDM and IBM (p<0.0001) while VCAM-1 expression in muscle fibers was almost absent in the four groups (p=0.2632). These findings emphasize the importance of adhesion molecules in the pathophysiology of the inflammatory myopathies, mainly the marked ICAM-1 expression in vessels in JDM, corroborating the microvascular involvement in this disease. In contrast, VCAM-1 seems not to play a major role in JDM, as previously described in PM, DM and IBM. Adhesion molecule expression in JDM presents a differential characteristic when compared to PM, DM and IBM.
authors.affiliation: Department of Pediatrics--University of São Paulo Medical School, Brazil. adrianasallum@ig.com.br
authors.forename: Adriana M E
authors.initials: AM
authors.lastname: Sallum
authors.forename: Maria H B
authors.initials: MH
authors.lastname: Kiss
authors.forename: Clovis A A
authors.initials: CA
authors.lastname: Silva
authors.forename: Alda
authors.initials: A
authors.lastname: Wakamatsu
authors.forename: Maria A A G
authors.initials: MA
authors.lastname: Vianna
authors.forename: Silvana
authors.initials: S
authors.lastname: Sachetti
authors.forename: Suely K N
authors.initials: SK
authors.lastname: Marie
mesh_terms: D000328:Adult; D001808:Blood Vessels / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D002648:Child; D003882:Dermatomyositis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D009220:Myositis / Q000378:metabolism* / Q000473:pathology; D018979:Myositis, Inclusion Body / Q000378:metabolism / Q000473:pathology; D017285:Polymyositis / Q000378:metabolism / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000378:metabolism*
chemical_list: D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1