Functional assay of EFA6A, a guanine nucleotide exchange factor for ADP-ribosylation factor 6 (ARF6), in dendritic formation of hippocampal neurons.
EFA6A is a guanine nucleotide exchange factor (GEF) that can activate ADP-ribosylation factor 6 (ARF6) in vitro, with prominent expression in the forebrain including the hippocampal formation. In this section, we describe the neuronal transfection method and show that the overexpression of a catalytically inactive mutant of EFA6A induces a prominent dendritic formation of the primary hippocampal neurons, suggesting the intimate involvement of EFA6A in the regulation of neuronal dendritic development. This reliable and consistent neuronal transfection method will also be applicable for the vector-based RNA interference method.
Sakagami
Hiroyuki
H
Department of Cell Biology, Tohoku University Graduate School of Medicine, Sendai, Japan.
Kamata
Akifumi
A
Fukunaga
Kohji
K
Kondo
Hisatake
H
D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D000818:Animals; D003712:Dendrites / Q000737:chemistry*; D004337:Drug Carriers; D005260:Female; D005333:Fetus / Q000166:cytology; D020662:Guanine Nucleotide Exchange Factors / Q000032:analysis*; D006624:Hippocampus / Q000166:cytology*; D008055:Lipids; D009474:Neurons / Q000737:chemistry*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection / Q000379:methods*
D000089982:ADP-Ribosylation Factor 6; D004337:Drug Carriers; D020662:Guanine Nucleotide Exchange Factors; D008055:Lipids; C086724:Lipofectamine; C504474:Psd protein, mouse; C504475:Psd protein, rat; D020727:ADP-Ribosylation Factors; C000715727:Arf6 protein, mouse; C000715349:Arf6 protein, rat