Identification and characterization of two internal cleavage and polyadenylation sites of parvovirus B19 RNA.
Polyadenylation of B19 pre-mRNAs at the major internal site, (pA)p1, is programmed by the nonconsensus core cleavage and polyadenylation specificity factor-binding hexanucleotide AUUAAA. Efficient use of this element requires both downstream and upstream cis-acting elements and is further influenced by an adjacent AAUAAC motif. The primary hexanucleotide element must be nonconsensus to allow efficient readthrough of P6-generated pre-mRNAs into the capsid-coding region. An additional cleavage and polyadenylation site, (pA)p2, 296 nucleotides downstream of (pA)p1 was shown to be used following both B19 infection and transfection of a genomic clone. RNAs polyadenylated at (pA)p2 comprise approximately 10% of B19 RNAs that are polyadenylated internally.
Yoto
Yuko
Y
Department of Molecular Microbiology and Immunology, 471f Life Sciences Center, University of Missouri-Columbia, 1201 E. Rollins Rd., Columbia, MO 65211-7310, USA.
Qiu
Jianming
J
Pintel
David J
DJ
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D019556:COS Cells; D002522:Chlorocebus aethiops; D006801:Humans; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000378:metabolism*; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
D012322:RNA Precursors; D012367:RNA, Viral