title: Effects of the enamel matrix derivative and beta-tricalcium phosphate on bone augmentation within a titanium cap in rabbit calvarium.
abstract: In vitro studies suggest that enamel matrix derivative (EMD) affects the early stages of osteogenic maturation by stimulating bone cell proliferation. In the present study, we evaluated the effects of EMD and beta-tricalcium phosphate (beta-TCP) on bone augmentation within a titanium cap in rabbit calvaria, using 14 adult male Japanese white rabbits. The calvarium was exposed, a circular groove prepared, the marrow penetrated, and a standard hemispherical titanium cap placed in the groove. The cap was filled with a mixture of beta-TCP and EMD at the experimental site, and was filled with beta-TCP alone at the control site. At 1 and 3 months after cap implantation, animals were euthanized, and histological sections prepared. The sections were stained with basic fuchsin and methylene blue, and were examined using light microscopy. At 1 month, EMD tended to increase the amount of bone, but there was no significant difference in the amount of new tissue and mineralized bone between the experimental and control sites. The present findings indicate that the present mixture of EMD and beta-TCP does not accelerate bone formation, compared with beta-TCP alone.
authors.affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan. murai-m@dent.nihon-u.ac.jp
authors.forename: Masakazu
authors.initials: M
authors.lastname: Murai
authors.forename: Shuichi
authors.initials: S
authors.lastname: Sato
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Koshi
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ikeda
authors.forename: Masataka
authors.initials: M
authors.lastname: Narukawa
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Takayama
authors.forename: Naoto
authors.initials: N
authors.lastname: Yoshinuma
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001853:Bone Marrow / Q000473:pathology / Q000601:surgery; D001857:Bone Matrix / Q000187:drug effects / Q000473:pathology; D002113:Calcification, Physiologic / Q000187:drug effects; D002130:Calcium Phosphates / Q000627:therapeutic use*; D004396:Coloring Agents; D003746:Dental Enamel Proteins / Q000627:therapeutic use*; D005456:Fluorescent Dyes; D006253:Haversian System / Q000187:drug effects / Q000473:pathology; D008297:Male; D008751:Methylene Blue; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects*; D011817:Rabbits; D012394:Rosaniline Dyes; D012886:Skull / Q000473:pathology / Q000601:surgery*; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D004396:Coloring Agents; D003746:Dental Enamel Proteins; D005456:Fluorescent Dyes; D012394:Rosaniline Dyes; C485817:beta-tricalcium phosphate; C109069:enamel matrix proteins; C025485:basic fuchsin; D008751:Methylene Blue