Enhanced immunogenicity of microencapsulated multiepitope DNA vaccine encoding T and B cell epitopes of foot-and-mouth disease virus in mice.
The role of poly(D,L-lactic-co-glycolic acid, PLGA) microparticles on enhancing immune responses of multiepitope DNA vaccines was investigated in vitro and in vivo. pcDNA-SG encoding T and B cell epitopes of foot-and-mouth disease virus (FMDV) was encapsulated into PLGA microparticles. PLGA microparticles could protect themselves from nuclease degradation in vitro. PLGA-pcDNA-SG microparticles could be uptaken by cells and expressed His-tagged SG immunogen in vitro and in vivo. A prolonged expression and presentation of SG immunogen were observed by confocal laser scanning microscopy in the lymphocytes from the mice incubated with PLGA-pcDNA-SG microparticles, compared with the mice immunized with naked pcDNA-SG. PLGA-pcDNA-SG microparticles displayed a significant stronger immunogenicity than naked DNA vaccines with a higher titer of virus-specific antibody, elevated IFN-gamma production and enhanced lymphocyte proliferation. PLGA-DNA microparticle could elicit augmented humoral and cellular responses with reduced amounts and times of immunization.
Wang
Fang
F
Department of Medical Genetics, The Second Millitary Medical University, No.800 Xiangyin Road, Yangpu District, Shanghai 200433, China.
He
Xiao-Wen
XW
Jiang
Lei
L
Ren
Ding
D
He
Ying
Y
Li
De-An
DA
Sun
Shu-Han
SH
D000818:Animals; D004339:Drug Compounding; D016503:Drug Delivery Systems; D018985:Epitopes, B-Lymphocyte / Q000235:genetics*; D018984:Epitopes, T-Lymphocyte / Q000235:genetics*; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics* / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects*; D051379:Mice; D049329:Nanostructures; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D019444:Vaccines, DNA; D014765:Viral Vaccines