[Cellular uptake behavior of antisense oligodeoxynucleotides polymethacrylate submicroparticles].
OBJECTIVE
To survey the uptake behavior and subcellular distribution of antisense oligodeoxynucleotide polymethacrylate submicroparticles (AS-ODN-SMP) and infer its mechanism in MGC cell lines.


METHODS
MGC cells were incubated at certain concentration of AS-ODN-SMP or AS-ODN for 8 h at 4 degrees C or 37 degrees C. Then the fluorescence oligodeoxynucleotide- labeled cells were counted by flow cytometer and the intracellular fluorescence intensity was determined after incubated with chloroquine for 2 h.


RESULTS
Cellular uptake of oligodeoxynucleotides was significantly increased following application of AS-ODN-SMP and total intracellular fluorescence intensity was enhanced by 683 folds with the vehicle concentration of 20 microg x mL(-1). AS-ODN-SMP entranced to cells profoundly with temperature-dependent manner. Rare cells took on fluorescence when incubated at 4 degrees C, while 37 degrees C they were significantly increased. But the intracellular fluorescence intensity appeared same level in present or absent of chloroquine.


CONCLUSIONS
With the help of polyacrylate submicroparticles, oligonucleotides efficiently entranced the cells via endocytosis and could successfully escape the degradation in lysosome.
Wang
Wen-Xi
WX
College of Pharmacy, Zhejiang University of Technology, Hangzhou 310014, China.
Liang
Wen-Quan
WQ
Song
Bi-Wei
BW
Chen
Hai-Liang
HL
D000818:Animals; D002460:Cell Line; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004705:Endocytosis / Q000187:drug effects*; D015726:Giant Cells / Q000166:cytology*; D008247:Lysosomes / Q000378:metabolism; D053758:Nanoparticles; D020319:Oligodeoxyribonucleotides, Antisense / Q000008:administration & dosage / Q000493:pharmacokinetics; D010316:Particle Size; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000494:pharmacology*; D013696:Temperature
D004337:Drug Carriers; D020319:Oligodeoxyribonucleotides, Antisense; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid