title: [Effect of interleukin-8 in cell invasion and proliferation of human breast cancer].
abstract: OBJECTIVE
  To identify the effect of interleukin-8 in cell progression and invasion of human breast cancer.
  
  
  METHODS
  Human cytokine antibody arrays were applied to screen a panel of cytokine expression from 11 human breast cancer cell lines, and the mechanism of identified key factors involved in breast cancer progression was studied.
  
  
  RESULTS
  Profiling of cytokine expression showed the expression of interleukin-8 was related to estrogen receptor status, metastasis and vimentin status in the 11 human breast cancer cell lines. Elevated expression of interleukin-8 in breast cancer cells had positive correlation with breast cancer invasion. Neutralization of antibody against interleukin-8 specifically blocked interleukin-8-mediated cell invasion. However, anti-interleukin-8 antibody did not influence the proliferation of breast cancer cells.
  
  
  CONCLUSIONS
  Interleukin-8 may be the key factor involved in human breast cancer progression and invasion, and play an important role in cell invasion of breast cancer.
authors.affiliation: Center of Breast Disease, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.forename: Shen-ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Lü
authors.forename: Ruo-pan
authors.initials: RP
authors.lastname: Huang
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000096:biosynthesis / Q000502:physiology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D040081:Protein Array Analysis; D011960:Receptors, Estrogen / Q000378:metabolism; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D016209:Interleukin-8; D011960:Receptors, Estrogen; D016159:Tumor Suppressor Protein p53