Immunomodulatory effects of beta-glucan on neutrophil function in fathead minnows (Pimephales promelas Rafinesque, 1820).
Stimulatory effects of yeast beta-1,3-1,6-glucans on neutrophils have long been recognized, but effects of glucans on degranulation of primary granules in fish neutrophils have not been previously reported. Neutrophil function was monitored during in vitro and in vivo application of glucans to non- (NS), acute- (AS) and chronically stressed (CS) fish. beta-Glucan proved to be a strong and quick (80%, 2 min) stimulant of degranulation. Dietary glucan increased degranulation in NS fish, and prevented a decrease in AS fish. Degranulation in CS fish returned to NS levels 3 days after the glucan diet was fed. Fathead minnows appear to be a useful model to investigate neutrophil degranulation in fish exposed to different environmental conditions and immunomodulators. Use of beta-glucans in fish diets prior to AS and during chronic stress can enhance neutrophil function, potentially increasing disease resistance and survival rates after transportation or exposure to poor water quality.
Palić
Dusan
D
Department of Natural Resource, Ecology and Management, College of Agriculture, Iowa State University, 339 Science 2, Ames, IA 50011, USA. dulep@iastate.edu
Andreasen
Claire B
CB
Herolt
Dawn M
DM
Menzel
Bruce W
BW
Roth
James A
JA
D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects / Q000276:immunology; D003530:Cyprinidae / Q000276:immunology*; D007155:Immunologic Factors; D018375:Neutrophil Activation / Q000187:drug effects / Q000276:immunology*; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D009195:Peroxidase / Q000276:immunology / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects / Q000276:immunology; D013312:Stress, Physiological / Q000276:immunology / Q000662:veterinary; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D047071:beta-Glucans / Q000494:pharmacology*
D007155:Immunologic Factors; D047071:beta-Glucans; D009195:Peroxidase; D013755:Tetradecanoylphorbol Acetate