Longitudinal analysis of cryptosporidium species-specific immunoglobulin G antibody responses in Peruvian children.
Cryptosporidium species are ubiquitous in the environment and are frequently detected in the stools of children who live where sanitation conditions are poor. To better characterize the immune response to these parasites, we monitored immunoglobulin G (IgG) antibody levels in a cohort of children from Lima, Peru. Two new enzyme-linked immunosorbent assays based on the C. parvum (bovine, subtype IIa) Iowa strain 17-kDa and 27-kDa antigens were used to measure IgG antibody levels in longitudinal serum samples. Antibody responses were detected during infections with C. parvum, C. felis, and C. meleagridis and with four different subtypes of C. hominis. We also noted that the magnitude of the antibody response was related to the number of previous infections and that older children generally had higher levels of antibodies to the two C. parvum antigens. Antibody responses were not associated with infections with either Cyclospora sp. or Giardia sp. We believe the antibody assays will be important tools for monitoring the success of future public health interventions.
Priest
Jeffrey W
JW
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA. jpriest@cdc.gov
Bern
Caryn
C
Xiao
Lihua
L
Roberts
Jacquelin M
JM
Kwon
James P
JP
Lescano
Andres G
AG
Checkley
William
W
Cabrera
Lilia
L
Moss
Delynn M
DM
Arrowood
Michael J
MJ
Sterling
Charles R
CR
Gilman
Robert H
RH
Lammie
Patrick J
PJ
D000367:Age Factors; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology; D002648:Child; D003457:Cryptosporidiosis / Q000175:diagnosis / Q000276:immunology*; D016785:Cryptosporidium parvum / Q000145:classification / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007231:Infant, Newborn; D008137:Longitudinal Studies; D010568:Peru; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G