Segmenting fluid effect on PCR reactions in microfluidic platforms.
This paper evaluates the compatibility of segmenting fluids for two phase flow applications in biomedical microdevices. The evaluated fluids are chosen due to the variations in fluid properties and cost, while also reflecting their use in the recent literature. These segmenting fluids are examined to determine their compatibility with the Polymerase Chain Reaction (PCR), through controlled experiments. The results are the first to provide a quantitative measure of segmenting fluid compatibility with PCR.
Walsh
E J
EJ
Stokes Research Institute, University of Limerick, Castletroy, Ireland.
King
C
C
Grimes
R
R
Gonzalez
A
A
D005591:Chemical Fractionation / Q000379:methods*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D017022:Flow Injection Analysis / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*
D004247:DNA