Selective decrease of Na+/k+ -ATPase activity in the brain of hypothyroid rats.
The present work was performed in order to know if mild hypothyroidism in rats modifies the activity of the Na+/K+ -ATPase in different regions of the brain. Male Wistar rats (300-350 g) were randomly divided into three groups: (1) control group (n=8) drank tap water. (2) hypothyroid group (n=8) treated with 60 mg/kg of methimazole in drinking water; and (3) replaced group (n=8) treated with 60 mg/kg of methimazole plus 35 microg/kg of thyroid hormone (T3) in drinking water. After four weeks of treatment, the rats of all groups were sacrificed by decapitation. The cortex, amygdala, hippocampus and cerebellum were dissected and frozen at -70 degrees C until assay. For enzymatic assay, the tissues were homogenized. The Na+/K+ -ATPase activity was determined by quantifying inorganic phosphate after the samples were incubated with ATP in the presence and absence of 1 mM ouabain. The Na+/K+ -ATPase activity is expressed as pmoles Pi/hr/mg protein. The results showed that the Na+/K+ -ATPase activity in the cortex, amygdala and hippocampus, but not in cerebellum, was lower in hypothyroid group than in control group (p<0.05). The co-administration of methimazole and T3 avoided the decrease of Na+/K+ -ATPase activity, except in amygdala. According to the results obtained we concluded that methimazole treatment decreased the Na+/K+- ATPase activity in the brain's regions which are related to seizures onset. That decrement in enzyme activity was avoided with the coadministration of thyroid hormone.
Pacheco-Rosado
Jorge
J
Depto. de Fisiología Mauricio Russek, Escuela Nacional de Ciencias Biológicas, I.P.N., Carpio y Plan de Ayala, México, D.F., 11340. Jpacheco@encb.ipn.mx
Arias-Citalán
G
G
Ortiz-Butrón
R
R
Rodríquez-Páez
L
L
D000818:Animals; D013956:Antithyroid Agents; D001831:Body Temperature / Q000502:physiology; D001921:Brain / Q000201:enzymology*; D007037:Hypothyroidism / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D008713:Methimazole; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013963:Thyroid Hormones / Q000502:physiology
D013956:Antithyroid Agents; D013963:Thyroid Hormones; D008713:Methimazole; D000254:Sodium-Potassium-Exchanging ATPase