title: Mitotic activity of Sertoli cells in adult human testis: an immunohistochemical study to characterize Sertoli cells in testicular cords from patients showing testicular dysgenesis syndrome.
abstract: During puberty, normal somatic Sertoli cells undergo dramatic morphological changes due to the differentiation of immature pre-Sertoli cells in functionally active adult Sertoli cells. Sertoli cell maturation is accompanied with loss of their mitotic activity before onset of spermatogenesis and loss of pre-pubertal and occurrence of adult immunohistochemical Sertoli cell differentiation markers. Testes of infertile adult patients often exhibit numerous histological signs of testicular dysgenesis syndrome (TDS) such as microliths, Sertoli cell only (SCO) tubules, tubules containing carcinoma in situ and immature seminiferous tubules (Sertoli cell nodules). Sertoli cell tumours, however, are very rare neoplasms possibly due to the fact that the mechanism and temporal origin of neoplastic Sertoli cells underlying Sertoli cell tumourigenesis still remain unknown. To clarify the state of Sertoli cell differentiation in both immature seminiferous tubules of adult patients with TDS and Sertoli cell tumour, we compared the expression of the Sertoli cell differentiation markers vimentin, inhibin-alpha, anti-Muellerian-hormone, cytokeratin 18, M2A-antigen, androgen receptor and connexin43 with that of SCO tubules with hyperplasia. In addition, we demonstrated for the first time the existence of proliferating Sertoli cells by Ki67- and PCNA-immunostaining in Sertoli cell nodules of the adult human testis. Our data indicate that mitotically active Sertoli cells in Sertoli cell nodules will be arrested prior to puberty and, contrary to dogma, do not represent foetal or neonatal cells. Since all markers in Sertoli cell nodules revealed a staining pattern identical to that in neoplastic Sertoli cells, but different to that in Sertoli cells of SCO tubules with hyperplasia, it may be speculated that Sertoli cell tumours in adult men may originate from Sertoli cell nodules.
authors.affiliation: Institute of Veterinary Anatomy, Histology and Embryology, University of Giessen, Giessen, Germany. Ralph.H.Brehm@vetmed.uni-giessen.de
authors.forename: Ralph
authors.initials: R
authors.lastname: Brehm
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Rey
authors.forename: Sabine
authors.initials: S
authors.lastname: Kliesch
authors.forename: Klaus
authors.initials: K
authors.lastname: Steger
authors.forename: Alexander
authors.initials: A
authors.lastname: Marks
authors.forename: Martin
authors.initials: M
authors.lastname: Bergmann
mesh_terms: D000328:Adult; D002648:Child; D006059:Gonadal Dysgenesis / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008938:Mitosis / Q000502:physiology*; D012707:Sertoli Cell Tumor / Q000473:pathology; D012708:Sertoli Cells / Q000166:cytology* / Q000473:pathology; D013085:Spermatic Cord / Q000166:cytology* / Q000473:pathology; D013577:Syndrome; D013737:Testis / Q000166:cytology* / Q000473:pathology