title: Protective effects of guarana (Paullinia cupana Mart. var. Sorbilis) against DEN-induced DNA damage on mouse liver.
abstract: Guarana (Paullinia cupana Mart. var. Sorbilis) is a plant originally from Brazil, which is rich in tannins. Some tannins are known to present protective effects against DNA damage. This study was performed to investigate the anti-genotoxic/cytotoxic properties of guarana in hepatocytes of mice injected with N-nitrosodiethylamine (DEN). The protective effect of guarana was evaluated both by comet assay and DNA smear fragmentation technique in two month-old female BALB/c mice. These were treated previously with 2.0 mg/g bw of guarana for 16 days and then injected with DEN (160 microg/g body weight) to induce DNA damage. The DEN-only treated group presented higher comet image length than the guarana plus DEN and untreated groups (116.06+/-5.0 microm, 104.09+/-3.3 microm and 93.28+/-14.4 microm, respectively; p<0.01). Guarana treatment presented a 52.54% reduction in comet image length when animals were exposed to DEN (p<0.05). DNA samples from the guarana plus DEN group clearly showed less EtBr fluorescence intensity when compared to the DEN-only group, reinforcing the comet assay data. These results show, for the first time, that guarana has a protective effect against DEN-induced DNA damage in mouse liver.
authors.affiliation: Laboratory of Experimental Oncology, Department of Pathology, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, CEP 05508-900 São Paulo, SP, Brazil.
authors.forename: H
authors.initials: H
authors.lastname: Fukumasu
authors.forename: J L
authors.initials: JL
authors.lastname: Avanzo
authors.forename: R
authors.initials: R
authors.lastname: Heidor
authors.forename: T C
authors.initials: TC
authors.lastname: Silva
authors.forename: A
authors.initials: A
authors.lastname: Atroch
authors.forename: F S
authors.initials: FS
authors.lastname: Moreno
authors.forename: M L Z
authors.initials: ML
authors.lastname: Dagli
mesh_terms: D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D053938:DNA Fragmentation; D004032:Diet; D004052:Diethylnitrosamine / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D032126:Paullinia / Q000737:chemistry; D013634:Tannins / Q000494:pharmacology
chemical_list: D000477:Alkylating Agents; D013634:Tannins; D004052:Diethylnitrosamine