Evaluation of the antioxidant properties and bioavailability of free and bound phenolic acids from Trichilia emetica Vahl.
Trichilia emetica Vahl. is commonly used in folk medicine of Mali for the treatment of various diseases. In this study, the content and the antioxidant activity of phenolic acids from Trichilia emetica root were evaluated. Free phenolic acids were extracted with a mixture of methanol and 10% acetic acid. Bound phenolic acids were released using first alkaline and then acid hydrolysis. All fractions were quantified separately by HPLC. After alkaline hydrolysis, a remarkable increase in caffeic acid, ferulic acid, p-coumaric acid, syringic acid, vanillic acid, protocathecuic acid and gallic acid content was observed, showing that most of phenolic acids in the drug are present as bound forms. Moreover, the extracts submitted to alkaline hydrolysis showed high antioxidant properties in two in vitro assays: autooxidation of methyl linoleate (MeLo) and ascorbate/Fe(2+)-mediated lipid peroxidation in rat microsomes. An in vivo study was also performed to investigate the intestinal absorption of phenolic acids after oral administration of Trichilia emetica extracts. Results showed high levels of phenolic acids, free or conjugated to glucuronide, in the plasma of rats treated with the hydrolyzed extract. Due to the absence of chlorogenic acid in plasma samples, the presence of caffeic acid seems to be derived from hydrolysis of chlorogenic acid in the gastrointestinal tract.
Germanò
M P
MP
Pharmaco-Biological Department, School of Pharmacy, University of Messina, Vill. SS. Annunziata, 98168 Messina, Italy. germamp@pharma.unime.it
D'Angelo
V
V
Biasini
T
T
Sanogo
R
R
De Pasquale
R
R
Catania
S
S
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001682:Biological Availability; D002109:Caffeic Acids / Q000493:pharmacokinetics / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000493:pharmacokinetics / Q000494:pharmacology; D005707:Gallic Acid / Q000493:pharmacokinetics / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000493:pharmacokinetics / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D029582:Meliaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011422:Propionates; D051381:Rats; D017208:Rats, Wistar; D014641:Vanillic Acid / Q000493:pharmacokinetics / Q000494:pharmacology
D000975:Antioxidants; D002109:Caffeic Acids; D003373:Coumaric Acids; D062385:Hydroxybenzoates; D010936:Plant Extracts; D011422:Propionates; D005707:Gallic Acid; D014641:Vanillic Acid; C495469:p-coumaric acid; C040048:caffeic acid