Epstein-Barr virus (EBV) infection and p53 protein expression in gastric carcinoma.
In the presented studies p53 protein expression was evaluated in samples of gastric carcinoma originating from 32 selected adult patients (with documented diagnosis of adenocarcinoma of the stomach and without the presence of Helicobacter pylori infection). Among the patients 14 individuals carried EBV-positive gastric carcinoma (group 1) while the 18 remaining patients carried EBV-negative gastric carcinoma (group 2). EBV infection was detected testing the tissue material for the presence of EBER by RNA in situ hybridization (ISH) and testing sera of the patients for EBV-specific antibodies. Expression of p53 protein was analysed using immunohistochemistry. Presence of p53 protein was noted in 9 (64.3%) cases of EBV-positive gastric cancer (group 1) and in 10 (55.5%) cases of EBV-negative gastric cancer (group 2). No significant differences were detected in the frequencies of p53 protein expression in the two studied groups. The results permit to conclude that abnormalities in p53 in gastric cancer are independent of EBV infection, even if EBV may participate in development of the tumour.
Szkaradkiewicz
Andrzej
A
University of Medical Sciences in Poznań, Wieniawskiego 3, 61-712 Poznań, Poland. szkaradkiewicza@poczta.onet.pl
Majewski
Wacław
W
Wal
Marzena
M
Czyzak
Mirosław
M
Majewski
Przemysław
P
Bierła
Joanna
J
Kuch
Alicja
A
D000230:Adenocarcinoma / Q000821:virology*; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D020031:Epstein-Barr Virus Infections / Q000150:complications*; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis; D013274:Stomach Neoplasms / Q000821:virology*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
D000914:Antibodies, Viral; C099537:Epstein-Barr virus encoded RNA 1; D012367:RNA, Viral; D016159:Tumor Suppressor Protein p53