title: Multiplexed measurement of serum antibodies using an array biosensor.
abstract: The array biosensor provides the capability for simultaneously measuring titers of antibody against multiple antigens. Human antibodies against four different targets, tetanus toxin, diphtheria toxin, staphylococcal enterotoxin B (SEB) and hepatitis B, were measured simultaneously in sera from eight different donors in a single assay and titers were determined. The assays could measure amounts of bound antibody as low as approximately 100 fg. Each individual serum exhibited a different pattern of reactivity against the four target antigens. Applications of this biosensor capability include monitoring for exposure to pathogens and for efficacy of vaccination.
authors.affiliation: Department Química Analítica, Facultad de Química, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Moreno-Bondi
authors.forename: Chris Rowe
authors.initials: CR
authors.lastname: Taitt
authors.forename: Lisa C
authors.initials: LC
authors.lastname: Shriver-Lake
authors.forename: Frances S
authors.initials: FS
authors.lastname: Ligler
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D015374:Biosensing Techniques; D004167:Diphtheria Toxin / Q000097:blood; D004768:Enterotoxins / Q000097:blood; D006041:Goats; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D040081:Protein Array Analysis; D013744:Tetanus Toxin / Q000097:blood
chemical_list: D000907:Antibodies, Bacterial; D004167:Diphtheria Toxin; D004768:Enterotoxins; D007074:Immunoglobulin G; D013744:Tetanus Toxin; C031818:enterotoxin B, staphylococcal