title: Association of interleukin-1beta gene polymorphisms with precancerous gastric lesions in African Americans and Caucasians.
abstract: OBJECTIVE
  Interleukin-1beta plays an important role in inflammation and gastric physiology. Polymorphisms of the IL1B gene have been associated with gastric atrophy and increased cancer risk, especially in Helicobacter pylori-infected subjects. The aim of this study was to evaluate the relationship between IL1B and IL1 receptor antagonist gene polymorphisms and the risk of multifocal atrophic gastritis in African Americans and Caucasians.
  
  
  METHODS
  Genomic DNA was extracted from gastric biopsies of 269 adult outpatients (172 African Americans and 97 Caucasians) undergoing diagnostic upper gastrointestinal endoscopy. Histological diagnosis was evaluated according to the updated Sydney System and H. pylori status was assessed by Steiner silver stain. Polymorphisms of the IL1B gene (-511, -31, and +3954) and the IL1 receptor antagonist were investigated by PCR-RFLP. Logistic regression models were used to identify variables associated with multifocal atrophic gastritis in terms of odds ratios and 95% confidence intervals.
  
  
  RESULTS
  Considering subjects with normal histology and nonatrophic gastritis as controls, a significant association was found between IL1B+3954T carrier and multiatrophic gastritis (OR 2.23, 95% CI 1.28, 3.88). Analyses stratified by ethnic group demonstrated similar associations in both African Americans (OR 2.23, 95% CI 1.14, 4.37) and Caucasians (OR 2.04, 95% CI 0.74, 5.65). A positive but not significant association was found between the allele 2 of the IL1RN and the presence of multifocal atrophic gastritis. The remaining proinflammatory polymorphisms were not associated with this precancerous lesion.
  
  
  CONCLUSIONS
  Our results suggest that the presence of IL1B+3954T allele is a risk marker for multifocal atrophic gastritis in the population studied.
authors.affiliation: Department of Pathology, Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA.
authors.forename: Jovanny
authors.initials: J
authors.lastname: Zabaleta
authors.forename: M Constanza
authors.initials: MC
authors.lastname: Camargo
authors.forename: M Blanca
authors.initials: MB
authors.lastname: Piazuelo
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Fontham
authors.forename: Barbara G
authors.initials: BG
authors.lastname: Schneider
authors.forename: Liviu A
authors.initials: LA
authors.lastname: Sicinschi
authors.forename: William
authors.initials: W
authors.lastname: Ferrante
authors.forename: Luis
authors.initials: L
authors.lastname: Balart
authors.forename: Pelayo
authors.initials: P
authors.lastname: Correa
authors.forename: Augusto C
authors.initials: AC
authors.lastname: Ochoa
mesh_terms: D000328:Adult; D001741:Black or African American / Q000235:genetics; D000368:Aged; D001707:Biopsy, Needle; D015331:Cohort Studies; D005260:Female; D005757:Gastritis, Atrophic / Q000208:ethnology* / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease / Q000208:ethnology*; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology; D011336:Probability; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D044465:White People / Q000235:genetics
chemical_list: D007375:Interleukin-1