Stability of the BK polyomavirus genome in renal-transplant patients without nephropathy.
To clarify the stability of the BK polyomavirus (BKPyV) genome in renal transplant (RT) recipients, three to five complete BKPyV genomes from each of six RT recipients with surviving renal allografts were molecularly cloned. The complete sequences of these clones were determined and compared in each patient. No nucleotide difference was detected among clones in two patients, and a few nucleotide variations were found among those in four patients. In each of these patients a parental sequence (usually the major sequence), from which variant sequences (usually minor sequences) with nucleotide substitutions would have been generated, were identified. A comparison between the parental and variant sequences in each patient identified a single nucleotide substitution in each variant sequence. From these findings, it was concluded that the genome of BKPyV is stable in RT recipients without nephropathy, with only minor nucleotide substitutions in the coding region.
Takasaka
Tomokazu
T
Department of Urology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Goya
Nobuyuki
N
Department of Urology, Tokyo Women's Medical University, Tokyo, Japan.
Ishida
Hideki
H
Department of Urology, Tokyo Women's Medical University, Tokyo, Japan.
Tanabe
Kazunari
K
Department of Urology, Tokyo Women's Medical University, Tokyo, Japan.
Toma
Hiroshi
H
Department of Urology, Tokyo Women's Medical University, Tokyo, Japan.
Fujioka
Tomoaki
T
Department of Urology, Iwate Medical University School of Medicine, Morioka, Japan.
Omori
So
S
Department of Urology, Iwate Medical University School of Medicine, Morioka, Japan.
Zheng
Huai-Ying
HY
Japanese Foundation for AIDS Prevention, Tokyo, Japan.|Department of Urology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Chen
Qin
Q
Department of Urology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Nukuzuma
Souichi
S
Kobe Institute of Health, Kobe, Japan.
Kitamura
Tadaichi
T
Department of Urology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Yogo
Yoshiaki
Y
Department of Urology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
D001739:BK Virus / Q000235:genetics* / Q000502:physiology; D036022:Capsid Proteins / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000150:complications; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D007674:Kidney Diseases / Q000821:virology; D016030:Kidney Transplantation; D008969:Molecular Sequence Data; D010802:Phylogeny
D036022:Capsid Proteins; C062481:VP1 protein, polyomavirus