title: Light-activated destruction of cancer cell nuclei by platinum diazide complexes.
abstract: A possible way to avoid dose-limiting side effects of platinum anticancer drugs is to employ light to cause photochemical changes in nontoxic platinum prodrugs that release active antitumor agents. This strategy could be used in the treatment of localized cancers accessible to irradiation (e.g., bladder, lung, esophagus, and skin). We report here that nontoxic photolabile diam(m)ino platinum(IV) diazido complexes inhibit the growth of human bladder cancer cells upon irradiation with light, and are non-crossresistant to cisplatin. Their rate of photolysis closely parallels that of DNA platination, indicating that the photolysis products interact directly, and rapidly, with DNA. Photoactivation results in a dramatic shrinking of the cancer cells, loss of adhesion, packing of nuclear material, and eventual disintegration of their nuclei, indicating a different mechanism of action from cisplatin.
authors.affiliation: Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, University of Greifswald, Germany. bednarsk@uni-greifswald.de
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Bednarski
authors.forename: Renate
authors.initials: R
authors.lastname: Grünert
authors.forename: Michael
authors.initials: M
authors.lastname: Zielzki
authors.forename: Anja
authors.initials: A
authors.lastname: Wellner
authors.forename: Fiona S
authors.initials: FS
authors.lastname: Mackay
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Sadler
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D001386:Azides / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects* / Q000528:radiation effects*; D004247:DNA / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D008027:Light; D015394:Molecular Structure; D009369:Neoplasms / Q000473:pathology*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D010984:Platinum / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D013057:Spectrum Analysis; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D001386:Azides; D017319:Photosensitizing Agents; D010984:Platinum; D004247:DNA