title: Towards functional glycomics by localization of binding sites for tissue lectins: lectin histochemical reactivity for galectins during diethylstilbestrol-induced kidney tumorigenesis in male Syrian hamster.
abstract: Endogenous lectins act as effectors of cellular activities such as growth regulation, migration, and adhesion. Following their immunohistochemical localization in our previous study (Saussez et al. in Histochem Cell Biol 123:29-41, 2005) we purified several galectins and used them as tools for monitoring accessible binding sites. Herein, we report the use of galectin histochemistry for the analysis of diethylstilbestrol (DES)-induced renal tumors in male Syrian hamster kidney (SHKT). Sections of normal kidney and DES-treated kidney were analyzed with biotinylated galectins-1, -3 (full-length and truncated), and -7. Accessible binding sites were detected, localization was predominantly extracellular and confined to medium-sized and large tumors. Monitoring the SHKT-derived HKT-1097 line, processed in vitro or as xenograft material, cytoplasmic and nuclear staining for galectins-1, -3, and -3tr could be observed. Adaptation of SHKT cells to long-term growth in culture is thus associated with emergence of this signal. Our data set illustrates the feasibility to complement immunohistochemical data by application of the tissue lectins as probes, and to detect regulation of galectin reactivity with differential characteristics within tumor progression in vivo and unique features of the tumor cell line in vitro and in vivo.
authors.affiliation: Laboratory of Histology, Faculty of Medicine and Pharmacy, University of Mons-Hainaut, Avenue du Champ de Mars, 6-Pentagone 1B, 7000, Mons, Belgium. sven.saussez@umh.ac.be
authors.forename: Sven
authors.initials: S
authors.lastname: Saussez
authors.forename: Francois
authors.initials: F
authors.lastname: Lorfevre
authors.forename: Denis
authors.initials: D
authors.lastname: Nonclercq
authors.forename: Guy
authors.initials: G
authors.lastname: Laurent
authors.forename: Sabine
authors.initials: S
authors.lastname: André
authors.forename: Fabrice
authors.initials: F
authors.lastname: Journé
authors.forename: Robert
authors.initials: R
authors.lastname: Kiss
authors.forename: Gérard
authors.initials: G
authors.lastname: Toubeau
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Gabius
mesh_terms: D000818:Animals; D001665:Binding Sites; D001710:Biotin / Q000378:metabolism; D002273:Carcinogens; D002460:Cell Line; D045744:Cell Line, Tumor; D006224:Cricetinae; D004054:Diethylstilbestrol; D005455:Fluorescent Antibody Technique; D037483:Galectin 1 / Q000378:metabolism; D037502:Galectin 3 / Q000378:metabolism; D037161:Galectins / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D037102:Lectins; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
chemical_list: D002273:Carcinogens; D037483:Galectin 1; D037502:Galectin 3; D037161:Galectins; D037102:Lectins; D001710:Biotin; D004054:Diethylstilbestrol