Microstructured liposome array.
Conversion of a DNA chip to a nanocapsule array was performed by grafting on a liposome an oligonucleotide complementary to an oligonucleotide bound to the array. Each liposome may be loaded by a soluble molecule or may present a hydrophobic or amphiphilic molecule inserted in its wall. To detect liposomes on the chip, we used fluorescent dyes encapsulated in the liposome internal volume or fluorescent lipids. We observed that an oligonucleotide-grafted liposome containing a defined dye specifically accumulated on the area where its complementary oligonucleotide had been spotted on the array. The virtually unlimited amount of addresses allows the specific binding of large amounts of liposomes in one single batch.
Chaize
Barnabé
B
Institut de Pharmacologie et de Biologie Structurale, 205, route de Narbonne 31077 Toulouse Cedex, France.
Nguyen
Michel
M
Ruysschaert
Tristan
T
le Berre
Véronique
V
Trévisiol
Emmanuelle
E
Caminade
Anne-Marie
AM
Majoral
Jean Pierre
JP
Pratviel
Geneviève
G
Meunier
Bernard
B
Winterhalter
Mathias
M
Fournier
Didier
D
D008081:Liposomes / Q000737:chemistry*; D036103:Nanotechnology; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry*
D008081:Liposomes; D009841:Oligonucleotides