An alloplasmic male-sterile line of Brassica oleracea harboring the mitochondria from Diplotaxis muralis expresses a novel chimeric open reading frame, orf72.
Nuclear so-called fertility-restorer genes reverse the pollen sterility of cytoplasmic male-sterile (CMS) plants caused by disturbed mitochondrial-nuclear interactions. We identified a CMS-associated chimeric mitochondrial gene in an alloplasmic CMS line of Brassica oleracea in the 'mur' system. This novel chimeric gene, orf72, was found in the mitochondrial genome of donor cytoplasm. It was located downstream of normal rps7 and contained part of atp9 (atp9-b). It was expressed specifically on the nuclear background of CMS B. oleracea, partially suppressed in the fertility-restored line and entirely suppressed in the cytoplasmic donor.
Shinada
Tomotaka
T
Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555 Japan.
Kikuchi
Yosuke
Y
Fujimoto
Ryo
R
Kishitani
Sachie
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000145:classification / Q000235:genetics*; D019607:Brassicaceae / Q000145:classification / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D050259:Genes, Mitochondrial; D017343:Genes, Plant; D018745:Genome, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis
D004272:DNA, Mitochondrial; D018744:DNA, Plant; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins