G-CSF loaded biodegradable PLGA nanoparticles prepared by a single oil-in-water emulsion method.
A new formulation method was developed for preparing poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles loaded with recombinant human granulocyte colony-stimulating factor (rhG-CSF). Lyophilized rhG-CSF powder and PLGA polymer were directly co-dissolved in a single organic phase, and the resulting solution was dispersed into an aqueous solution. PLGA nanoparticles encapsulating rhG-CSF were produced by a spontaneous emulsion/solvent diffusion method. In this manner, rhG-CSF was molecularly dissolved in the polymer phase. Release profile of rhG-CSF from PLGA nanoparticles was compared with those from two kinds of PLGA microparticles which were separately prepared by either single oil-in-water (O/W) or double water-in-oil-in-water (W/O/W) emulsion technique. The sizes of rhG-CSF loaded nanoparticles, O/W microparticles, and W/O/W microparticles were about 257 nm, 4.7 microm, and 4.3 microm, respectively. For rhG-CSF nanoparticles, about 90% of encapsulated rhG-CSF was released out in a sustained manner from PLGA nanoparticles over a 1 week period, but for rhG-CSF microparticles, only about 20% of rhG-CSF could be released out during the same period. Reversed phase and size exclusion chromatograms revealed that the structural integrity of released rhG-CSF from nanoparticles was nearly intact, compared to that of native rhG-CSF.
Choi
Seung Ho
SH
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea.
Park
Tae Gwan
TG
D002626:Chemistry, Pharmaceutical; D003692:Delayed-Action Preparations; D004355:Drug Stability; D004655:Emulsions; D016179:Granulocyte Colony-Stimulating Factor / Q000737:chemistry* / Q000235:genetics; D019344:Lactic Acid / Q000737:chemistry*; D049329:Nanostructures; D036103:Nanotechnology; D009821:Oils / Q000737:chemistry; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods; D013997:Time Factors; D014867:Water / Q000737:chemistry
D003692:Delayed-Action Preparations; D004655:Emulsions; D009821:Oils; D011108:Polymers; D011994:Recombinant Proteins; D014867:Water; D016179:Granulocyte Colony-Stimulating Factor; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid