title: Effects of single administration of diethylstilbestrol on murine langerhans cells.
abstract: Diethylstilbestrol (DES) is a synthetic compound with potent estrogenic actions useful in the treatment of prostate carcinoma despite the fact that it can also induce some forms of neoplasia. Both effects are thought to be related to its estrogenic actions and very little attention has been focused on the possible effect of DES on the immune response. Skin is the largest organ of the body and constitutes the first line of defense against xenobiotics. The Skin Immune System (SIS) has become the center of attention of research for the development of new therapeutic approaches for neoplasic diseases. Langerhans cells (LC), as an element of SIS, are "professional" antigen presenting cells resident in the skin that participate in the immune response associated with tolerance and acquired immunity to antigens. Hence in this work we studied the effect of DES on LC of murine skin as a model to analyze the possible effect of DES on the immune response. Male CD-1 mice (20 to 35 g body weight) were treated topically (TO) or subcutaneously (SC) with DES (10 and 100 mg/kg, dissolved in ethanol) and sacrificed at 12, 84 and 228 hr. LC were quantified in the ear skin of mice using both an enzymatic histochemical technique to demonstrate ATP-ase activity; and an indirect immunohistochemical assay for detecting class II molecules of the major histocompatibility complex (MHC-II). DES induced a significant time- and dose- dependent reduction in the number of LC (P < 0.05). Data presented here suggest that estrogens may exert a modulatory action on LC.
authors.affiliation: School of Medicine, National Autonomous University of Mexico.
authors.forename: Ana Martha
authors.initials: AM
authors.lastname: Hernandez-Segura
authors.forename: Isai
authors.initials: I
authors.lastname: Ayala-Garcia
authors.forename: S Judith
authors.initials: SJ
authors.lastname: Alvarez-Pérez
authors.forename: Andres E
authors.initials: AE
authors.lastname: Castell-Rodriguez
authors.forename: Beatriz
authors.initials: B
authors.lastname: Hernandez-Tellez
authors.forename: Juan Jose
authors.initials: JJ
authors.lastname: Mandoki
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Ramirez-Gonzalez
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects; D045166:Estradiol Congeners / Q000494:pharmacology*; D005802:Genes, MHC Class II; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D007801:Langerhans Cells / Q000187:drug effects*; D008297:Male; D051379:Mice; D009336:Necrosis; D015430:Weight Gain / Q000187:drug effects
chemical_list: D002273:Carcinogens; D045166:Estradiol Congeners; D004054:Diethylstilbestrol; D000251:Adenosine Triphosphatases