[Studies on the seed embryo germination and propagation of Dendrobium candidum in vitro].
OBJECTIVE
To determine optimum culture conditions for the seed embryo culture and rapid propagation of Dendrobium candidum.


METHODS
Seed embryos of D. candidum were incubated in the medium containing a combination of 6-benzylaminopurine (BA) and 1-naphthaleneacetic acid (NAA), potato extract, banana extract and activated carbon in order to induce seed embryo germination, protocorm differentiation, plantlet propagation and plantlet rooting.


CONCLUSIONS
The maximum embryo germination percentage was obtained in the 1/2 MS media supplemented with 20% potato extract. The 1/2 MS medium supplemented with 1.0 mg x L(-1) BA and 0.1 mg x L(-1) NAA was very beneficial to the protocorm differentiation and propagation of D. candidum. The highest protocorm propagation index was obtained from the medium containing the activated carbon. The highest root numbers and length were observed in plants growing in 1/2 MS medium containing 0.5 mg x L(-1) NAA.
Tang
Gui-xiang
GX
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China. tanggx@zju.edu.cn
Wang
Fu-deng
FD
Zhou
Wei-jun
WJ
D001593:Benzyl Compounds; D002244:Carbon / Q000494:pharmacology; D003470:Culture Media; D031667:Dendrobium / Q000254:growth & development*; D018525:Germination / Q000187:drug effects*; D007701:Kinetin / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000254:growth & development*; D011687:Purines; D012639:Seeds / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
D001593:Benzyl Compounds; D003470:Culture Media; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D002244:Carbon; C480551:benzylaminopurine; D007701:Kinetin