Standardization of a very specific and sensitive single PCR for detection of Plasmodium vivax in low parasitized individuals and its usefulness for screening blood donors.
Here, we describe the standardization of a very sensitive and specific single Plasmodium vivax polymerase chain reaction (PCR) and its usefulness for diagnosis and screening procedures when a Plasmodium falciparum PCR was also utilized. The P. vivax PCR sensitivity threshold was 0.019 parasites per microliter, and a PCR fragment was only detected when P. vivax DNA was present. Among the 11 febrile patients with negative parasitological examination that attended the malaria service of Fundação de Medicina Tropical do Amazonas, we diagnosed one P. vivax malaria by PCR. Among the 286 individuals considered suitable for blood donation, we also detected by PCR an individual with P. vivax malaria, and conversely, we did not detect any malaria infection in blood donor candidates considered unsuitable due to its past malaria history. We conclude that PCR is the method of choice for low-parasitized individuals and could therefore represent a complementary tool to safely rescue blood donor candidates considered unsuitable on the basis of malaria history.
Torres
Kátia Luz
KL
Department of Immunology, Instituto Oswaldo Cruz, FioCruz, Av Brasil 4365, Rio de Janeiro, 22045-900, Brazil.
Figueiredo
Daniel Vasquez
DV
Zalis
Mariano Gustavo
MG
Daniel-Ribeiro
Cláudio Tadeu
CT
Alecrim
Wilson
W
Ferreira-da-Cruz
Maria de Fátima
Mde F
D000818:Animals; D001782:Blood Donors; D005335:Fever of Unknown Origin / Q000469:parasitology; D006801:Humans; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000469:parasitology; D008403:Mass Screening / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000592:standards*; D010963:Plasmodium falciparum / Q000235:genetics / Q000302:isolation & purification; D010966:Plasmodium vivax / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012680:Sensitivity and Specificity