title: Mitomycin C augmented glaucoma surgery: evolution of filtering bleb avascularity, transconjunctival oozing, and leaks.
abstract: OBJECTIVE
  To prospectively study the evolution of possible high risk features of conjunctival filtration blebs like avascularity, transconjunctival oozing (TCO), and leaks after mitomycin C (MMC) enhanced glaucoma surgery.
  
  
  METHODS
  Single observer, 2 year prospective study on bleb characteristics of 125 eyes of 125 consecutive patients who had MMC augmented glaucoma surgery with initially successful filtration. MMC (0.2 mg/ml for 2 minutes in most cases) was applied on the area of the scleral flap before dissection. Glaucoma surgeries included were trabeculectomy, deep sclerectomy, and combined procedures. A dry fluorescein strip was applied on the avascular part of the bleb and observed for aqueous egress with flow (point leak, PL) or without (TCO).
  
  
  RESULTS
  The mean time (95% CI) for observing bleb avascularity, TCO, and bleb leaks were 106 days (69 to 143), 208 days (155 to 261), and 609 days (559 to 659), respectively. Bleb leaks were observed in 17 eyes (13.6%)-15 (24.6%) in the trabeculectomy group and two (3.1%) in the deep sclerectomy group (p = 0.003). Kaplan-Meier survival analyses showed that the probability of observing bleb avascularity at sixth, 12th, and 24th month after surgery was 56%, 71%, and 73%, respectively. In eyes with avascular blebs, the probability of developing TCO and leaks was 77% and 1% at 6 months, 81% and 12% at 12 months, and 95% and 26% at 24 months, respectively. Cox's regression analyses and log rank tests showed that eyes with larger avascular blebs (>4 mm) were more likely to develop TCO (hazard ratio 3.77, p = 0.001) and delayed bleb leaks were more likely to be seen in eyes of the trabeculectomy group rather than the deep sclerectomy group (hazard ratio 0.06, p = 0.0006).
  
  
  CONCLUSIONS
  MMC application over the area of scleral flap dissection during glaucoma surgery is associated with a high incidence of bleb avascularity, TCO, and delayed bleb leaks. Most eyes developed bleb avascularity within the first year after surgery. TCO will eventually be seen in all eyes with avascular blebs and the incidence of leaks gradually increases with time. This study shows that patients with eyes undergoing glaucoma surgery with MMC and avascular blebs should be monitored indefinitely.
authors.affiliation: Department of Ophthalmology, Huddersfield Royal Infirmary, Lindley, Huddersfield HD3 3EA, UK. nitin.anand@lycos.co.uk
authors.forename: N
authors.initials: N
authors.lastname: Anand
authors.forename: S
authors.initials: S
authors.lastname: Arora
authors.forename: M
authors.initials: M
authors.lastname: Clowes
mesh_terms: D000368:Aged; D001768:Blister / Q000139:chemically induced* / Q000473:pathology; D003228:Conjunctiva / Q000098:blood supply; D003229:Conjunctival Diseases / Q000139:chemically induced* / Q000473:pathology; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage; D005901:Glaucoma / Q000473:pathology / Q000601:surgery*; D006801:Humans; D007429:Intraocular Pressure / Q000187:drug effects; D008297:Male; D016685:Mitomycin / Q000009:adverse effects*; D011183:Postoperative Complications; D011446:Prospective Studies; D012590:Sclera / Q000601:surgery; D016019:Survival Analysis; D013997:Time Factors; D014130:Trabeculectomy / Q000379:methods; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects
chemical_list: D016685:Mitomycin; D005472:Fluorouracil