title: Pectic polysaccharide isolated from Angelica gigas Nakai inhibits melanoma cell metastasis and growth by directly preventing cell adhesion and activating host immune functions.
abstract: The pectic polysaccharide (angelan) of Angelica gigas Nakai is an immunostimulator that activates the immune functions of B cells and macrophages. Here we investigated the effect of angelan on tumor growth and metastasis. Angelan was found to significantly prolong the survival rate of B16F10-implanted mice and to reduce the frequency of pulmonary metastasis of B16F10 melanoma. Moreover, the combined treatment of angelan and doxorubicin (a cytotoxic anticancer agent) more effectively inhibited tumor growth and metastasis than either compound alone. In the present study, we found that angelan directly inhibited cancer cell adhesion and invasion through the extracellular matrix, in addition to activating the immune functions of B cells and macrophages. These results suggest that angelan can inhibit tumor growth and metastasis by stimulating host immunity and directly inhibiting cancer cell adhesion.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 52 Oundong, Yusong, Taejon 305-333, South Korea.
authors.forename: Sang-Bae
authors.initials: SB
authors.lastname: Han
authors.forename: Chang Woo
authors.initials: CW
authors.lastname: Lee
authors.forename: Moo Rim
authors.initials: MR
authors.lastname: Kang
authors.forename: Yeo Dae
authors.initials: YD
authors.lastname: Yoon
authors.forename: Jong Soon
authors.initials: JS
authors.lastname: Kang
authors.forename: Ki Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Won Kee
authors.initials: WK
authors.lastname: Yoon
authors.forename: Kiho
authors.initials: K
authors.lastname: Lee
authors.forename: Song-Kyu
authors.initials: SK
authors.lastname: Park
authors.forename: Hwan Mook
authors.initials: HM
authors.lastname: Kim
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D007274:Injections, Intraperitoneal; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008546:Melanoma, Experimental / Q000401:mortality / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D028321:Plant Preparations / Q000008:administration & dosage / Q000494:pharmacology; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D015996:Survival Rate; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D028321:Plant Preparations; D011134:Polysaccharides; C515883:angelan; D004317:Doxorubicin