Engineered membrane superchannel improves bioremediation potential of dioxin-degrading bacteria.
Sphingomonas sp. A1 possesses specialized membrane structures termed 'superchannels' that enable the direct incorporation of macromolecules into the cell. We have engineered two related sphingomonads, the dioxin-degrading S. wittichii RW1 and the polypropylene glycol-degrading S. subarctica IFO 16058(T), to incorporate this superchannel into their cell membranes. In both cases the bioremediation capability of the organisms was substantially increased pointing at the potential of this approach as a general strategy to improve bacterial degradation of hazardous compounds in the environment.
Aso
Yuji
Y
Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
Miyamoto
Yukiko
Y
Harada
Karen Mine
KM
Momma
Keiko
K
Kawai
Shigeyuki
S
Hashimoto
Wataru
W
Mikami
Bunzo
B
Murata
Kousaku
K
D001673:Biodegradation, Environmental; D004147:Dioxins / Q000302:isolation & purification / Q000493:pharmacokinetics*; D024861:Genetic Enhancement / Q000379:methods*; D007220:Industrial Waste / Q000517:prevention & control; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D020578:Sphingomonas / Q000235:genetics* / Q000378:metabolism*
D004147:Dioxins; D007220:Industrial Waste; D007473:Ion Channels; D011994:Recombinant Proteins