The effect of anti-human CD134 monoclonal antibody on phytohemagglutinin-induced mRNA expression of perforin in peripheral blood mononuclear cells.
CD134, a member of the tumor necrosis factor receptor superfamily, plays a crucial role in T cell survival. In this study, peripheral blood mononuclear cells (PBMCs) stimulated with phytohemagglutinin (PHA, 50 microg/ml) were treated by CD134 mAb (1 microg/ml, 5 microg/ml, 10 microg/ml) for 6 h, 12 h, 24 h and 48 h. The level of perforin mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR) technique. Our data showed that the expression of perforin mRNA in PBMCs was down-regulated by CD134 mAb in a dose-dependent manner in range of 1 microg/ml to 5 microg/ml and dropped down to its minimum on 24 h (p<0.05). The level of perforin mRNA reached a plateau when the concentration of CD134 mAb exceeded 5 microg/ml. In conclusion, CD134 mAb can inhibit perforin expression, which may enhance the ability of T cells for survival.
Li
Ming
M
Department of Rheumatology and Immunology, Shandong Provincial Hospital, Shandong University, Jinan 250021, China.
Zhang
Yuanchao
Y
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics*; D054353:Perforin; D010835:Phytohemagglutinins / Q000494:pharmacology*; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053262:Receptors, OX40; D018124:Receptors, Tumor Necrosis Factor / Q000276:immunology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D010835:Phytohemagglutinins; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger; D053262:Receptors, OX40; D018124:Receptors, Tumor Necrosis Factor; C505716:TNFRSF4 protein, human; D054353:Perforin