The immunogenicity of human adipose-derived cells: temporal changes in vitro.
Regenerative medical techniques will require an abundant source of human adult stem cells that can be readily available at the point of care. The ability to use unmatched allogeneic stem cells will help achieve this goal. Since adipose tissue represents an untapped reservoir of human cells, we have compared the immunogenic properties of freshly isolated, collagenase-digested human adipose tissue-derived stromal vascular fraction cells (SVFs) relative to passaged, plastic-adherent adipose-derived stem cells (ASCs). Parallel studies have shown that adherence to plastic and subsequent expansion of human adipose-derived cells selects for a relatively homogeneous cell population based on immunophenotype. Consistent with these findings, the presence of hematopoietic-associated markers (CD11a, CD14, CD45, CD86, and histocompatible locus antigen-DR [HLA-DR]) detected on the heterogeneous SVF cell population decreased upon subsequent passage of the ASCs. In mixed lymphocyte reactions (MLRs), SVFs, and early passage ASCs stimulated proliferation by allogeneic responder T cells. In contrast, the ASCs beyond passage P1 failed to elicit a response from T cells. Indeed, late passage ASCs actually suppressed the MLR response. Although these results support the feasibility of allogeneic human ASC transplantation, confirmatory in vivo animal studies will be required.
McIntosh
Kevin
K
Cognate Therapeutics, Inc., Baltimore, Maryland, USA.
Zvonic
Sanjin
S
Garrett
Sara
S
Mitchell
James B
JB
Floyd
Z Elizabeth
ZE
Hammill
Lora
L
Kloster
Amy
A
Di Halvorsen
Yuan
Y
Ting
Jenny P
JP
Storms
Robert W
RW
Goh
Brian
B
Kilroy
Gail
G
Wu
Xiying
X
Gimble
Jeffrey M
JM
D017667:Adipocytes / Q000166:cytology / Q000276:immunology*; D000273:Adipose Tissue / Q000166:cytology* / Q000276:immunology; D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D002469:Cell Separation; D002478:Cells, Cultured; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D017154:Stromal Cells / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D015703:Antigens, CD