Fatty acid alterations in liver peroxisomes from n-3-deficient mice.
A diet deficient in n-3 fatty acids dramatically reduces docosahexaenoic acid (4.8-fold) and 20:5n-3 content in murine total peroxisomal phospholipids, and conversely increases 22:5n-6 (17.1-fold) and also, to a lesser extent, 20:4n-6. This was also found in purified phosphatidylethanolamine and phosphatidylcholine. After changing the non-deficient diet (containing alpha-linolenic acid, ALA) to a deficient one (deficient in ALA), it took a very long time for docosahexaenoic acid concentration in peroxisomes to decline (>5 months). In contrast, after changing the deficient to a non-deficient diet, time to complete recovery was more rapid (3 weeks). Changes in 20:5n-3, 22:6n-3 and 20:4n-6 were generally stabilized within 2-4 weeks. Dietary n-3 fatty acids control the fatty acid composition of peroxisomal membranes, and thus possibly affect some of their functions.
Bourre
Jean-Marie
JM
INSERM U705, CNRS UMR 7157, Universités Paris 7 et 5, Hôpital Fernand Widal, Paris, France. jean-marie.bourre@fwidal.inserm.fr
Dumont
Odile
O
Clément
Michel
M
D000409:Alanine / Q000008:administration & dosage / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000378:metabolism*; D051379:Mice; D020675:Peroxisomes / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000378:metabolism; D011897:Random Allocation; D013997:Time Factors; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000378:metabolism
D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D017962:alpha-Linolenic Acid; D004281:Docosahexaenoic Acids; C483858:phosphatidylethanolamine; D000409:Alanine