Changes in composition and content of mycolic acids in glutamate-overproducing Corynebacterium glutamicum.
Overproduction of glutamate by Corynebacterium glutamicum is induced by biotin limitation or by the supplementation of specific detergents, sublethal amounts of penicillin, or cerulenin. But, it remains unclear why these different treatments, which have different sites of primary action, produce similar effects. In this study, it was found that the cellular content of mycolic acids--characteristic constituents of Corynebacterineae that are synthesized from fatty acids and form a cell surface layer--decreased under all conditions that induced glutamate overproduction. Furthermore, short mycolic acids increased under conditions of biotin limitation and cerulenin supplementation. These results suggest that different treatments produce the same effect that causes defects in the mycolic acid layer. This is perhaps one of the key factors in overproduction of glutamate by C. glutamicum.
Hashimoto
Ken-ichi
K
Department of Environmental Materials Science, Tokyo Denki University, Japan.
Kawasaki
Hisashi
H
Akazawa
Kouki
K
Nakamura
Jun
J
Asakura
Youko
Y
Kudo
Takuji
T
Sakuradani
Eiji
E
Shimizu
Sakayu
S
Nakamatsu
Tsuyoshi
T
D002569:Cerulenin / Q000494:pharmacology; D002849:Chromatography, Gas; D048230:Corynebacterium glutamicum / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D004952:Esters / Q000737:chemistry; D005971:Glutamates / Q000096:biosynthesis*; D013058:Mass Spectrometry; D008745:Methylation; D015394:Molecular Structure; D009171:Mycolic Acids / Q000737:chemistry* / Q000378:metabolism*
D004952:Esters; D005971:Glutamates; D009171:Mycolic Acids; D002569:Cerulenin