Effect of interleukin-18 on metastasis of mouse osteosarcoma cells.
The effect of interleukin-18 (IL-18) on metastasis of highly metastatic LM8 mouse osteosarcoma cells was investigated using nude mice treated with anti-asialo GM1 serum to exclude anti-tumor actions of IL-18 through activation of T and natural killer cells. Injection of LM8 cells which do not express IL-18 receptor beta into a tail vain resulted in the formation of pulmonary and hepatic metastatic foci. Daily injection of mice with IL-18 starting the fifth day from the cell injection had no significant effect on the number of metastatic foci, while five daily injections of IL-18 before and after the cell injection resulted in marked decreases. Culture of LM8 cells with IL-18 for 5 days before the injection into mice produced no significant effect on the number of pulmonary and hepatic metastatic foci. In contrast, pretreatment of mice with IL-18 for 5 days before the cell injection markedly decreased metastatic foci. The retention of LM8 cells in the lung 24 h after their injection was also reduced by the pretreatment of mice with IL-18. Serum obtained from mice pretreated with IL-18 for 5 days suppressed mobility of LM8 cells but IL-18 itself did not. These results suggest that IL-18 inhibits metastasis of LM8 cells partly by inducing a factor(s) in the host which suppresses cell mobility.
Nakamura
Yoshiteru
Y
Department of Pathology, Hyogo College of Medicine, 663-8501 Nishinomiya, Hyogo, Japan.
Yamada
Naoko
N
Ohyama
Hideki
H
Nakasho
Keiji
K
Nishizawa
Yasuko
Y
Okamoto
Takuya
T
Futani
Hiroyuki
H
Yoshiya
Shinichi
S
Okamura
Haruki
H
Terada
Nobuyuki
N
D000818:Animals; D017209:Apoptosis; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000276:immunology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004354:Drug Screening Assays, Antitumor; D007167:Immunotherapy / Q000379:methods; D020382:Interleukin-18 / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000556:secondary; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000556:secondary; D008297:Male; D051379:Mice; D008819:Mice, Nude; D012516:Osteosarcoma / Q000188:drug therapy* / Q000276:immunology
D020382:Interleukin-18