Identification and analysis of Ku70 and Ku80 homologs in the koji molds Aspergillus sojae and Aspergillus oryzae.
Ku genes play a key role in the non-homologous end-joining pathway. We have identified Ku70 and Ku80 homologs in the koji molds Aspergillus sojae and Aspergillus oryzae, and have constructed the disruption mutants of Ku70, Ku80, and Ku70-80 to characterize the phenotypic change in these mutants. Neither Ku70- nor Ku80-disrupted strains show hypersensitivity to the DNA damaging agents methylmethane sulfonate (MMS) and phleomycin. Moreover, undesirable phenotypes, such as poor growth or repressed conidiospore formation, were not observed in the Ku-disrupted A. sojae and A. oryzae.
Takahashi
Tadashi
T
Noda Institute for Scientific Research, Noda City, Chiba, Japan. ttakahashi@mail.kikkoman.co.jp
Masuda
Tsutomu
T
Koyama
Yasuji
Y
D000595:Amino Acid Sequence; D034961:Antigens, Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001230:Aspergillus / Q000737:chemistry / Q000378:metabolism*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005822:Genetic Vectors / Q000235:genetics; D000072200:Ku Autoantigen; D008954:Models, Biological; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D016415:Sequence Alignment
D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D000072200:Ku Autoantigen