title: Two-dimensional mass spectrometric mapping.
abstract: The identification and relative quantification of proteins in closely related biological samples is the backbone for many investigations in systems biology and for the discovery of biomarkers. While two-dimensional gel-based methodologies are still widely used for comparative proteomic studies, the recent advent of gel-free methodologies may allow the analysis of a larger number of samples in an automated fashion. Most of the technologies presented in this review require a chemical modification of proteins before analysis, and rely on the relative intensities of mass spectrometry signals for protein quantification. In particular, two-dimensional mass spectrometric mapping methodologies provide a visual representation of mass spectrometric data, thus facilitating the identification of differences in relative protein abundance.
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, Zürich, Wolfgang-Pauli-Str. 10, ETH-Hönggerberg, 8093 Zürich, Switzerland.
authors.forename: Christoph
authors.initials: C
authors.lastname: Roesli
authors.forename: Giuliano
authors.initials: G
authors.lastname: Elia
authors.forename: Dario
authors.initials: D
authors.lastname: Neri
mesh_terms: D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods; D012984:Software
chemical_list: D010455:Peptides; D011506:Proteins