Municipal landfill leachate induces cytogenetic damage in root tips of Hordeum vulgare.
With increasing use of municipal solid waste landfills for solid waste disposal, municipal landfill leachate has become a serious concern for aquatic environments. Therefore, it is important to study its toxic effects on plants, animals, and humans. In the present study, the genotoxicity of leachate was investigated with the Hordeum vulgare root-tip cytogenetic bioassay, using Chemical oxygen demand (COD(Cr)) as a measure of leachate concentration. Leachate decreased the mitotic index (MI) in a concentration-dependent and a time-dependent manner. Compared with the negative control, reductions of MI of 37%, 56%, and 70% were detected for the highest leachate concentration (COD(Cr) 800 mg/L) in root tips treated for 24, 48, and 72 h, respectively. Leachate caused significant increases of micronucleus (MCN) frequencies in a concentration-dependent and a time-dependent manner. Among the exposure groups, COD(Cr) 40 mg/L leachate induced MCN formation after 24, 48, and 72 h of exposure, and the frequencies of MCN significantly increased with increasing concentrations of leachate from COD(Cr) 40 to 640 mg/L. In addition, pycnotic cells (PNC) occurred in root tips at all leachate concentrations tested after 24, 48, and 72 h of exposure, and PNC frequencies had a positive relation with the treatment concentration and the exposure time. The results also indicate that the concentration required to produce cytogenetic damage was lower in cells that had a longer exposure time. The results confirm that components of leachate may be genotoxic in plant cells and imply that long exposure to leachate at low concentrations in the aquatic environment may pose a potential genotoxic risk to organisms. The results suggest that the H. vulgare cytogenetic bioassay is efficient, simple, and reproducible in genotoxicity studies of leachate, and that there appears to be a correlation between the genotoxicity in a plant system and the COD(Cr) of leachate.
Sang
Nan
N
Department of Environmental Science, College of Environment and Resource, Shanxi University, Taiyuan, Shanxi 030006, People's Republic of China.
Li
Guangke
G
Xin
Xiaoyun
X
D002453:Cell Cycle / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology; D001467:Hordeum / Q000166:cytology / Q000187:drug effects*; D007220:Industrial Waste / Q000009:adverse effects*; D015162:Micronucleus Tests; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index; D009153:Mutagens / Q000633:toxicity*; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects*; D012989:Soil Pollutants / Q000633:toxicity*
D007220:Industrial Waste; D009153:Mutagens; D012989:Soil Pollutants