Proteomic analysis of growth phase-dependent proteins of Streptococcus pneumoniae.
Streptococcus pneumoniae is an important human pathogen that causes a variety of diseases, such as pneumonia, bacteremia, meningitis, otitis media, and sinusitis, in both adults and children. The global pattern of growth phase-dependent protein expression of S. pneumoniae during in vitro culture was analyzed using 2-DE combined with MALDI-TOF MS and LC/ESI-MS/MS. Several protein production patterns were observed at four time points throughout the growth stage, although some protein levels did not change significantly. We focused on the switch in protein expression at the transition from log growth phase to stationary phase. Proteins that were significantly induced or repressed at this point are likely to be involved in central intermediary metabolism, amino acid synthesis, nucleotide, and fatty acid metabolism, cell wall synthesis, protein degradation, and stress responses. This global expression profiling approach has revealed previously unrecognized relationships between proteins in the life of this pathogen.
Lee
Kwang-Jun
KJ
Division of Bacterial Respiratory Infections, Department of Microbiology, National Institute of Health, Korea Center for Diseases Control and Prevention, Eunpyung-Ku, Seoul, Korea.
Bae
Song-Mee
SM
Lee
Myoung-Ro
MR
Yeon
Sun-Mi
SM
Lee
Young-Hee
YH
Kim
Ki-Sang
KS
D001426:Bacterial Proteins / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013296:Streptococcus pneumoniae / Q000254:growth & development / Q000378:metabolism
D001426:Bacterial Proteins; D015415:Biomarkers; D020543:Proteome