Daboia russellii and Naja kaouthia venom neutralization by lupeol acetate isolated from the root extract of Indian sarsaparilla Hemidesmus indicus R.Br.
The present study reports the isolation and purification of lupeol acetate from the methanolic root extract of Indian medicinal plant Hemidesmus indicus (L.) R.Br. (family: Asclepiadaceae) which could neutralize venom induced action of Daboia russellii and Naja kaouthia on experimental animals. Lupeol acetate could significantly neutralize lethality, haemorrhage, defibrinogenation, edema, PLA(2) activity induced by Daboia russellii venom. It also neutralized Naja kaouthia venom induced lethality, cardiotoxicity, neurotoxicity and respiratory changes in experimental animals. Lupeol acetate potentiated the protection by snake venom antiserum action against Daboia russellii venom induced lethality in male albino mice. Venom induced changes in lipid peroxidation and super oxide dismutase activity was antagonized by lupeol acetate. Snake venom neutralization by lupeol acetate and its possible mechanism of action has been discussed.
Chatterjee
Ipshita
I
Laboratory of Toxinology and Experimental Pharmacodynamics, Department of Physiology, University of Calcutta, Calcutta 700009, India.
Chakravarty
A K
AK
Gomes
A
A
D000818:Animals; D000997:Antivenins / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D004546:Elapid Venoms / Q000037:antagonists & inhibitors* / Q000633:toxicity; D031102:Hemidesmus / Q000737:chemistry; D006470:Hemorrhage / Q000139:chemically induced / Q000188:drug therapy; D007194:India; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D009500:Neutralization Tests; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D032281:Smilax / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D014315:Triterpenes / Q000627:therapeutic use*; D014757:Viper Venoms / Q000037:antagonists & inhibitors* / Q000633:toxicity; D000094042:Lupanes
D000997:Antivenins; D004546:Elapid Venoms; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D013482:Superoxide Dismutase; D014315:Triterpenes; D014757:Viper Venoms; C010480:lupeol; D000094042:Lupanes