title: Effect of the urease accessory genes on activation of the Helicobacter pylori urease apoprotein.
abstract: The roles that accessory gene products play in activating the Helicobacter pylori urease apoprotein were examined. The activity of the urease apoprotein increased in the following order when it was expressed with the accessory genes: ureG<ureGH<ureFGH<ureEFGH<ureIEFGH. Moreover, stepwise additions of ureE and ureI to ureFGH significantly increased urease activity. Urease apoproteins coexpressed with ureFGH, ureEFGH, and ureIEFGH had similar low chymotrypsin susceptibilities. In vivo and in vitro activation studies showed that the cooperative effect of the accessory proteins involved processes in which the UreFGH complex, UreE, and UreI were implicated. Thus, the UreFGH complex may serve to alter the conformation of the apoprotein into one that is more competent to assemble a stable metallocenter, and that facilitates cooperative effects.
authors.affiliation: Department of Microbiology, Gyeongsang National University College of Medicine, Jinju 660-701, Korea.
authors.forename: Jeong-Uck
authors.initials: JU
authors.lastname: Park
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.forename: Young-Cheol
authors.initials: YC
authors.lastname: Kwon
authors.forename: Mi-Ja
authors.initials: MJ
authors.lastname: Chung
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Jun
authors.forename: Jeong-Won
authors.initials: JW
authors.lastname: Park
authors.forename: Seung-Gyu
authors.initials: SG
authors.lastname: Park
authors.forename: Hyang-Ran
authors.initials: HR
authors.lastname: Hwang
authors.forename: Sang-Haeng
authors.initials: SH
authors.lastname: Choi
authors.forename: Seung-Chul
authors.initials: SC
authors.lastname: Baik
authors.forename: Hyung-Lyun
authors.initials: HL
authors.lastname: Kang
authors.forename: Hee-Shang
authors.initials: HS
authors.lastname: Youn
authors.forename: Won-Kon
authors.initials: WK
authors.lastname: Lee
authors.forename: Myung-Je
authors.initials: MJ
authors.lastname: Cho
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Rhee
mesh_terms: D001059:Apoproteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004789:Enzyme Activation; D015964:Gene Expression Regulation, Bacterial; D016480:Helicobacter pylori / Q000201:enzymology*; D015151:Immunoblotting; D028044:Phosphate-Binding Proteins; D010957:Plasmids; D014510:Urease / Q000235:genetics / Q000378:metabolism*
chemical_list: D001059:Apoproteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D028044:Phosphate-Binding Proteins; C086767:UreD protein, Bacteria; C090404:UreE protein, Bacteria; C082270:ureG protein, Bacteria; D014510:Urease