[A new culture method for primordial germ cell].
To investigate the new method that the primordial germ cells (PGCs) can long time propagate, grow and keep potency of differentiation in vitro. PGCs were co-cultured with Sertoli cells (SCs) or with homologue fibroblasts from genital ridge. When co-cultured with SCs, PGCs colonies and times of transfer of culture were greater than those co-cultured with homologue fibroblasts. At present PGCs of co-cultured with SCs have succeeded in subculturing to the 51 generation. SCs can availably enhance propagating ability as well as a long period of time maintain the characteristic of PGCs.
Lu
Xin
X
Reproductive Medical Center,Capital University of Medical Sciences, Beijing 100069.
Xu
Qing
Q
Shi
Xiao Lin
XL
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005854:Germ Cells / Q000166:cytology* / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D012708:Sertoli Cells / Q000166:cytology / Q000502:physiology