title: [Human recombinant antibodies to variola virus].
abstract: Eight specific antibodies to live variola virus (VV), Ind-3a strain, and 7 antibodies to VV, Butler strain, were selected from the synthetic combinatorial phage display library on single-chain (scFv) human antibodies. Indirect solid-phase enzyme immunoassay showed the ability of these antibodies to bind the VV strains Ind-3a, Butler, Brazil-131, Kuw-5, and Congo-2. Moreover, earlier selected human scFv antibodies were also tested in the reaction of binding to the above VV strains. The experiments could reveal the antibodies that bound alastrim strains more effectively that did other VV strains. The nucleotide sequences encoding for the selected scFv antibodies were determined.
authors.forename: N V
authors.initials: NV
authors.lastname: Tikunova
authors.forename: E I
authors.initials: EI
authors.lastname: Bovshik
authors.forename: T E
authors.initials: TE
authors.lastname: Iun
authors.forename: E V
authors.initials: EV
authors.lastname: Zhirakovskaia
authors.forename: V V
authors.initials: VV
authors.lastname: Morozova
authors.forename: T A
authors.initials: TA
authors.lastname: Batanova
authors.forename: A A
authors.initials: AA
authors.lastname: Gus'kov
authors.forename: E B
authors.initials: EB
authors.lastname: Sokunova
authors.forename: A A
authors.initials: AA
authors.lastname: Il'ichev
authors.forename: L S
authors.initials: LS
authors.lastname: Sandakhchiev
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D020650:Combinatorial Chemistry Techniques; D003429:Cross Reactions; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D019151:Peptide Library; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012901:Variola virus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D011994:Recombinant Proteins