Equine CCL11 induces eosinophil cytoskeletal reorganization and activation.
OBJECTIVE
To assess the biological effects of purified recombinant equine CCL11 on equine eosinophil function.


METHODS
Following stimulation of eosinophils from normal horses, the polymerised form of actin was measured by flow cytometry using fluorescently labelled phalloidin. Migration was determined in a 96 well plate chemotaxis assay using 8 microm pore membranes, and adherence of eosinophils to serum-coated plastic was assessed using a colorimetric assay for eosinophil peroxidase. Superoxide generation was measured by the reduction of cytochrome C in a colorimetric assay.


RESULTS
Equine CCL11 induced significant (p < 0.001), concentration-dependent actin polymerisation and migration of equine eosinophils. Stimulation with CCL11 did not induce significant adherence to serum coated plastic, or superoxide production.


CONCLUSIONS
Equine CCL11 stimulates cytoskeletal reorganization and migration of equine eosinophils, suggesting that it may be involved in the regulation of eosinophil trafficking in horses.
Weston
M C
MC
Department of Veterinary Basic Sciences, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA, UK. mweston@rvc.ac.uk
Collins
M E
ME
Cunningham
F M
FM
D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D054413:Chemokine CCL11; D019742:Chemokines, CC / Q000096:biosynthesis* / Q000502:physiology*; D002631:Chemotactic Factors, Eosinophil / Q000378:metabolism; D002633:Chemotaxis; D003599:Cytoskeleton / Q000378:metabolism*; D004804:Eosinophils / Q000378:metabolism*; D005434:Flow Cytometry; D006736:Horses; D006801:Humans; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
D000199:Actins; C515456:CCL11 protein, human; D054413:Chemokine CCL11; D019742:Chemokines, CC; D002631:Chemotactic Factors, Eosinophil; D013481:Superoxides