Antioxidant and heme oxygenase-1 (HO-1)-induced effects of selected Taiwanese plants.
Recent studies have shown biological effects of heme oxygenase-1 (HO-1) induction and antioxidation in cardiovascular disorders. The ethanol extracts of leaves of 12 selected indigenous Taiwanese plants were investigated for their antioxidant activities, evaluated using assays of 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and superoxide radicals scavenging and reducing power activities as well as the induction of heme oxygenase-1 (HO-1). Acer albopurpurascens, Cinnamomum kanehirai, Diospyros discolor, Excoecaria kawakamii, Koelreuteria henryi, and Syzygium formosanum showed better DPPH-scavenging activities than the other plants. IC(50) values ranged from 1.7 to 8.7 microg/mL. Excepting Millettia pulchra var. microphylla and Pittosporum moluccanum, the extracts displayed hydroxyl-scavenging activities (IC(50) of 0.16-0.67 microg/mL). A. albopurpurascens, D. discolor, K. henryi, and S. formosanum also showed good superoxide anion radical scavenging activities and IC(50) values ranged from 12.9 to 28.5 microg/mL. D. discolor, K. henryi, and S. formosanum showed potent reducing power and M. pulchra var. microphylla and S. formosanum exhibited potent HO-1 induced activity. These active plant extracts also contained abundant phenolic constituents. The present results provide candidates to isolate the active constituents and develop natural antioxidants.
Lee
Mei-Hsien
MH
Graduate Institute of Pharmacognosy, Taipei Medical University, Taiwan. Lmh@tmu.edu.tw
Jiang
Cheun-Bin
CB
Juan
Shu-Hui
SH
Lin
Rong-Dih
RD
Hou
Wen-Chi
WC
D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds; D004790:Enzyme Induction / Q000187:drug effects; D000431:Ethanol / Q000737:chemistry; D051547:Heme Oxygenase-1 / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D017665:Hydroxyl Radical / Q000032:analysis; D020128:Inhibitory Concentration 50; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry* / Q000502:physiology; D010946:Plants, Medicinal / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D019163:Reducing Agents / Q000032:analysis; D013481:Superoxides / Q000032:analysis; D013624:Taiwan
D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D011994:Recombinant Proteins; D019163:Reducing Agents; D013481:Superoxides; D017665:Hydroxyl Radical; D000431:Ethanol; C004931:1,1-diphenyl-2-picrylhydrazyl; D008156:Luciferases; D051547:Heme Oxygenase-1