title: Cofilin mediates ATP depletion-induced endothelial cell actin alterations.
abstract: Ischemia and sepsis lead to endothelial cell damage, resulting in compromised microvascular flow in many organs. Much remains to be determined regarding the intracellular structural events that lead to endothelial cell dysfunction. To investigate potential actin cytoskeletal-related mechanisms, ATP depletion was induced in mouse pancreatic microvascular endothelial cells (MS1). Fluorescent imaging and biochemical studies demonstrated a rapid and progressive increase in F-actin along with a decrease in G-actin at 60 min. Confocal microscopic analysis showed ATP depletion resulted in destruction of actin stress fibers and accumulation of F-actin aggregates. We hypothesized these actin alterations were secondary to dephosphorylation/activation of actin-depolymerizing factor (ADF)/cofilin proteins. Cofilin, the predominant isoform expressed in MS1 cells, was rapidly dephosphorylated/activated during ATP depletion. To directly investigate the role of cofilin activation on the actin cytoskeleton during ischemia, MS1 cells were infected with adenoviruses containing the cDNAs for wild-type Xenopus laevis ADF/cofilin green fluorescent protein [XAC(wt)-GFP], GFP, and the constitutively active and inactive isoforms XAC(S3A)-GFP and XAC(S3E)-GFP. The rate and extent of cortical actin destruction and actin aggregate formation were increased in ATP-depleted XAC(wt)-GFP- and XAC(S3A)-GFP-expressing cells, whereas increased actin stress fibers were observed in XAC(S3E)-GFP-expressing cells. To investigate the upstream signaling pathway of ADF/cofilin, LIM kinase 1-GFP (LIMK1-GFP) was expressed in MS1 cells. Cells expressing LIMK1-GFP protein had higher levels of phosphorylated ADF/cofilin, increased stress fibers, and delayed F-actin cytoskeleton destruction during ATP depletion. These results strongly support the importance of cofilin regulation in ischemia-induced endothelial cell actin cytoskeleton alterations leading to cell damage and microvascular dysfunction.
authors.affiliation: Div. of Nephrology, Indiana Univ. School of Medicine, Indianapolis, IN 46202-5116, USA.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Suurna
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Ashworth
authors.forename: Melanie
authors.initials: M
authors.lastname: Hosford
authors.forename: Ruben M
authors.initials: RM
authors.lastname: Sandoval
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Wean
authors.forename: Bijal M
authors.initials: BM
authors.lastname: Shah
authors.forename: James R
authors.initials: JR
authors.lastname: Bamburg
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Molitoris
mesh_terms: D051339:Actin Depolymerizing Factors / Q000235:genetics / Q000502:physiology*; D000199:Actins / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000172:deficiency*; D000818:Animals; D002460:Cell Line; D042783:Endothelial Cells / Q000737:chemistry*; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics; D054461:Lim Kinases; D051379:Mice; D008833:Microcirculation / Q000166:cytology; D010179:Pancreas / Q000098:blood supply; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins; D014162:Transfection; D014982:Xenopus laevis / Q000235:genetics
chemical_list: D051339:Actin Depolymerizing Factors; D000199:Actins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D054461:Lim Kinases; C515893:Limk1 protein, mouse