Regulation of ILT3 gene expression by processing of precursor transcripts in human endothelial cells.
Immunoglobulin-like transcript (ILT)-3 is a transmembrane receptor, which belongs to the immunoglobulin superfamily. In previous studies, we showed that allospecific CD8+CD28- T suppressor cells (Ts) induce the expression of ILT3 in human endothelial cells (EC) rendering them tolerogenic. Using a polymerase chain reaction (PCR)-based approach, we now demonstrate by cell fractionation and sequencing studies that ILT3 precursor RNA is expressed and retained in nuclei of resting EC. Ts interaction with EC or exposure of EC to interleukin-10 (IL-10) and interferon alpha (IFN-alpha) triggers processing of ILT3 pre-mRNA. Western blot analysis showed that the expression of the mature ILT3 transcript is accompanied by production of ILT3 protein.
Kim-Schulze
S
S
Department of Pathology, Columbia University, New York, NY, USA.
Seki
T
T
Vlad
G
G
Scotto
L
L
Fan
J
J
Colombo
P C
PC
Liu
J
J
Cortesini
R
R
Suciu-Foca
N
N
D018106:CD28 Antigens / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D004730:Endothelium, Vascular / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008562:Membrane Glycoproteins; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D011971:Receptors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology; D014158:Transcription, Genetic
D018106:CD28 Antigens; C107907:LILRB4 protein, human; D008562:Membrane Glycoproteins; D012322:RNA Precursors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic