Dihydroxynonene mercapturic acid, a urinary metabolite of 4-hydroxynonenal, as a biomarker of lipid peroxidation.
The objective of our study was to compare the information obtained through the use of three different urinary biomarkers of lipoperoxidation during the time course of a bromotrichloromethane (BrCCl3) induced oxidative stress in rats. These biomarkers were malondialdehyde (MDA) measured by LC/MS after derivatization, the isoprostane 8-iso-PGF2alpha measured by enzyme immunoassay and 1,4-dihydroxynonene mercapturic acid (DHN-MA), the major 4-hydroxynonenal urinary metabolite [1], measured by LC-MS. Male Wistar rats received a single dose of 100 microL/kg BrCCl3 per os and lipid peroxidation was estimated every day for a 4-day-period after treatment. MDA, 8-iso-PGF2alpha and DHN-MA significantly increased in response to BrCCl3 treatment for this period of time, and DHN-MA showed the main increase during the 24-48 h period after treatment.
Peiro
Géraldine
G
UMR 1089-Xénobiotiques, INRA/ENVT, BP 3, 31931, Toulouse Cedex 9, France.
Alary
Jacques
J
Cravedi
Jean-Pierre
JP
Rathahao
Estelle
E
Steghens
Jean-Paul
JP
Guéraud
Françoise
F
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D000447:Aldehydes / Q000652:urine*; D000818:Animals; D015415:Biomarkers / Q000652:urine*; D001975:Bromotrichloromethane / Q000008:administration & dosage; D002853:Chromatography, Liquid; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D007700:Kinetics; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000652:urine; D013058:Mass Spectrometry; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar
C501116:1,4-dihydroxynonene mercapturic acid; D000447:Aldehydes; D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D008315:Malondialdehyde; D015237:Dinoprost; D001975:Bromotrichloromethane; C027576:4-hydroxy-2-nonenal; D000111:Acetylcysteine