Development of a faster determination of 10 anabolic steroids residues in animal muscle tissues by liquid chromatography tandem mass spectrometry.
A method had been developed for determination of residues of 10 anabolic steroids (ASs) in animal muscle tissues by liquid chromatography tandem mass spectrometry (LC/MS/MS). After enzymolysis, the sample was extracted with tert-butyl methyl ether, cleaned up through reverse solid-phase extraction and further determined by LC/MS/MS under multiple reaction monitoring (MRM) mode. The limits of detection (LOD) of LC/MS/MS method used for testing epitestosterone (ETS), nandrolone (17 beta-NT), 17 alpha-methyl-testosterone (MTS), testosterone 17-propionate (PTS), medroxyprogesterone (MED), progesterone (PG), estrone (ESN), 17 beta-estradiol (17 beta-ES), 17alpha-ethynylestradiol (EES) and estriol (EST) in animal muscle ranged from 0.06 to 0.22 microg/kg, and the limits of quantification (LOQ) were from 0.12 to 0.54 microg/kg. Experiments on spiked samples of pork, beef, chicken and fish showed that at addition level of 1.0 microg/kg, the average recoveries of the ASs ranged from 64% to 77%, and coefficients of variation from 7.1% to 20.3%, while at addition level of 2.0 microg/kg, the average recoveries ranged from 70% to 89%, and coefficient of variation from 7.1% to 19.1%.
Xu
Chuan Lai
CL
School of Food Science and Technology, Southern Yangtaze University, 214036 WuXi, JiangSu Province, China. xcl@sytu.edu.cn
Chu
Xiao Gang
XG
Peng
Chi Fang
CF
Jin
Zheng Yu
ZY
Wang
Li Ying
LY
D045930:Anabolic Agents / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D004845:Epitestosterone / Q000032:analysis; D005506:Food Contamination / Q000517:prevention & control; D005513:Food Inspection / Q000379:methods*; D008460:Meat / Q000032:analysis*; D008777:Methyltestosterone; D009132:Muscles / Q000737:chemistry*; D009277:Nandrolone / Q000032:analysis; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine
D045930:Anabolic Agents; D004845:Epitestosterone; D009277:Nandrolone; D008777:Methyltestosterone