title: Advances in proteomics data analysis and display using an accurate mass and time tag approach.
abstract: Proteomics has recently demonstrated utility for increasing the understanding of cellular processes on the molecular level as a component of systems biology approaches and for identifying potential biomarkers of various disease states. The large amount of data generated by utilizing high efficiency (e.g., chromatographic) separations coupled with high mass accuracy mass spectrometry for high-throughput proteomics analyses presents challenges related to data processing, analysis, and display. This review focuses on recent advances in nanoLC-FTICR-MS-based proteomics approaches and the accompanying data processing tools that have been developed to display and interpret the large volumes of data being produced.
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
authors.forename: Jennifer S D
authors.initials: JS
authors.lastname: Zimmer
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Monroe
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Qian
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
mesh_terms: D000818:Animals; D015415:Biomarkers; D001330:Electronic Data Processing / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D036103:Nanotechnology; D020543:Proteome; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D012984:Software; D013997:Time Factors
chemical_list: D015415:Biomarkers; D020543:Proteome