title: In vitro effects of Ag+ on planktonic and adhered cells of fluconazole-resistant and susceptible strains of Candida albicans, C. glabrata and C. krusei.
abstract: Planktonic and attached cells of strains of Candida albicans, C. glabrata and C. krusei with varied susceptibilities to fluconazole (FCZ) were compared for their relative susceptibilities to Ag+ via cell recovery and flow cytometric analyses. All strains lost membrane permeability and were non-recoverable upon culture after 1h exposure in morpholino-ethanesulfonic acid (MES) buffer fortified with <or= 2.0 microg/ml Ag+. Cells attached to silicone over a 2-h period demonstrated enhanced tolerance to FCZ and to a lesser degree to Ag+. Minimal inhibitory concentrations of Ag+ in defined media increased in the order C. glabrata, C. krusei, C. albicans. Susceptibilities to Ag+ did not correlate with tolerance or resistance to FCZ.
authors.affiliation: Biology Department, Georgia State University, Kell Hall, 24 Peachtree Center Avenue, Atlanta, GA 30303, USA. zhangshangtong@yahoo.com
authors.forename: Shangtong
authors.initials: S
authors.lastname: Zhang
authors.forename: Donald G
authors.initials: DG
authors.lastname: Ahearn
authors.forename: Carolina
authors.initials: C
authors.lastname: Mateus
authors.forename: Sidney A
authors.initials: SA
authors.lastname: Crow
mesh_terms: D000935:Antifungal Agents / Q000008:administration & dosage; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002175:Candida / Q000166:cytology* / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D025141:Drug Resistance, Fungal / Q000502:physiology; D004361:Drug Tolerance / Q000502:physiology; D015725:Fluconazole / Q000008:administration & dosage*; D012834:Silver / Q000008:administration & dosage*; D013045:Species Specificity
chemical_list: D000935:Antifungal Agents; D012834:Silver; D015725:Fluconazole