title: Crystallization and preliminary X-ray crystallographic analysis of PAS factor from Vibrio vulnificus.
abstract: Plasmid Achromobacter secretion (PAS) factor is a putative secretion factor that induces the secretion of periplasmic proteins. PAS factor from Vibrio vulnificus was crystallized at 294 K by the hanging drop vapor-diffusion method. It was isolated as a monomer during the purification procedures. The native crystal belongs to the F222 space group with unit cell parameters a=56.1, b=74.4, c=80.0 A, a=b=g=90 degrees. The crystal was soaked in cryoprotectant containing 1 M NaBr for 1 h for MAD phasing. The diffraction limit of the Br-MAD data set was 1.9 A using synchrotron X-ray irradiation at beam line BL-18B at the Photon Factory, Japan.
authors.affiliation: Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
authors.forename: Jun Hyuck
authors.initials: JH
authors.lastname: Lee
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Seong-Hwan
authors.initials: SH
authors.lastname: Rho
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Im
authors.forename: Young Ran
authors.initials: YR
authors.lastname: Kim
authors.forename: Mun-Kyoung
authors.initials: MK
authors.lastname: Kim
authors.forename: Gil Bu
authors.initials: GB
authors.lastname: Kang
authors.forename: Joon Haeng
authors.initials: JH
authors.lastname: Rhee
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Eom
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D041261:Vibrio vulnificus / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins