Molecular weight determination of peptides and proteins by ESI and MALDI.
Several topics are covered, namely, general aspects important for mass determination of peptides and proteins, sample preparation for both ESI and MALDI, and various mass analyzers coupled to these ionization techniques. Finally, the discussion is carried out on peptide and protein mass analysis as related to accuracy and precision of mass determination for both ESI-MS and MALDI-MS.
Strupat
Kerstin
K
Thermo Electron GmbH (Bremen), Germany.
D000818:Animals; D002138:Calibration; D004798:Enzymes / Q000737:chemistry; D006801:Humans; D007554:Isotopes; D013058:Mass Spectrometry; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D014466:Ultraviolet Rays
D004798:Enzymes; D007554:Isotopes; D010455:Peptides; D011506:Proteins