[Inhibiting effect of adenosine on platelet activation in vitro].
This study was aimed to investigate the inhibiting effects of adenosine on platelet in vitro in order to select functional protectants for platelets before lyophilization. Platelet membrane surface CD62P expression was assayed by flow cytometer (FCM). Platelet aggregations induced by restocetin, thrombin, ADP and propyl gallate were detected by APACT-2. The results showed that platelets membrane surface CD62P expression increased significantly after pre-treating of freeze-drying. 0.75 mmol/L adenosine could inhibit CD62P expression in a dose-dependent manner. Adenosine could inhibit platelet aggregation induced by propyl gallate, but no action on restocetin. When adenosine concentration was 1.0 mmol/L or higher, the aggregation induced by thrombin was significantly restrained. When concentration of adenosine was 0.75 mmol/L, platelet activation resulted from retreating could be inhibited and platelet aggregation induced by restocetin and thrombin were not affected markedly. It is concluded that adenosine can be one of the functional protectants and activation inhibitors in vitro for platelet cryo-preservation.
Wang
Dong-Mei
DM
Department of Blood Transfusion, Urumqi General Hospital, Lanzhou Military Area, Urumqi 830000, China.
Liu
Jing-Han
JH
Zhou
Jun
J
Ouyang
Xi-Lin
XL
Xing
Yan-Chao
YC
D000241:Adenosine / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D001793:Blood Preservation; D015925:Cryopreservation; D005434:Flow Cytometry; D006801:Humans; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*
D019007:P-Selectin; D010975:Platelet Aggregation Inhibitors; D000241:Adenosine