title: The determination of psilocin and psilocybin in hallucinogenic mushrooms by HPLC utilizing a dual reagent acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection system.
abstract: This paper describes a procedure for the determination of psilocin and psilocybin in mushroom extracts using high-performance liquid chromatography with postcolumn chemiluminescence detection. A number of extraction methods for psilocin and psilocybin in hallucinogenic mushrooms were investigated, with a simple methanolic extraction being found to be most effective. Psilocin and psilocybin were extracted from a variety of hallucinogenic mushrooms using methanol. The analytes were separated on a C12 column using a (95:5% v/v) methanol:10 mM ammonium formate, pH 3.5 mobile phase with a run time of 5 min. Detection was realized through a dual reagent chemiluminescence detection system of acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II). The chemiluminescence detection system gave improved detectability when compared with UV absorption at 269 nm, with detection limits of 1.2 x 10(-8) and 3.5 x 10(-9) mol/L being obtained for psilocin and psilocybin, respectively. The procedure was applied to the determination of psilocin and psilocybin in three Australian species of hallucinogenic mushroom.
authors.affiliation: School of Biological and Chemical Sciences, Deakin University, Geelong, VIC 3217, Australia.
authors.forename: Nicole
authors.initials: N
authors.lastname: Anastos
authors.forename: Simon W
authors.initials: SW
authors.lastname: Lewis
authors.forename: Neil W
authors.initials: NW
authors.lastname: Barnett
authors.forename: D Noel
authors.initials: DN
authors.lastname: Sims
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006213:Hallucinogens / Q000032:analysis*; D007202:Indicators and Reagents; D008163:Luminescent Measurements / Q000379:methods*; D009942:Organometallic Compounds; D011196:Potassium Permanganate; D011562:Psilocybin / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: D006213:Hallucinogens; D007202:Indicators and Reagents; D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D011196:Potassium Permanganate; D011562:Psilocybin; C009105:psilocin