title: Defective postnatal development of the male reproductive tract in LGR4 knockout mice.
abstract: The final outcome of tube elongation and branching is to maximize the epithelial exchange surfaces in tubular organs. The molecular and cellular basis of these processes is actively studied in model organs such as mammary glands, liver and kidney, but they remain almost unexplored in the male reproductive tract. Here, we report that the orphan G protein-coupled receptor LGR4/GPR48 plays a role in the postnatal tissue remodeling needed for elongation and convolution of the efferent ducts and epididymis. In LGR4 knockout male mice, tube elongation fails, resulting in a hypoplastic and poorly convoluted tract. Cell proliferation is dramatically reduced in KO affected tissues, providing an explanation to the observed phenotype. Detailed analysis showed that LGR4 inactivation manifests differently in the affected organs. In efferent ducts, immune cells infiltrate the epithelium and reach the lumen, blocking the transit of sperm and testicular fluid. In addition, the hypoplasia and low convolution result in a reduction of the epithelial area involved in liquid reabsorption. Both phenomena contribute in tissue swelling upstream the blockade due to liquid and sperm accumulation, with secondary damaging effects on the germinal epithelium. In the epididymis, the thin and highly convoluted duct is replaced by a large cystic tube which is surrounded by a thick condensation of mesenchymal cells. The abnormal organization of the cellular compartments in and around the ducts suggests that LGR4 might play a role in epithelial-mesenchymal interactions. Altogether, our data identify LGR4 as an important signaling molecule implicated in the tube morphogenesis of the male reproductive tract.
authors.affiliation: Institut de Recherche en Biologie Humaine et Moléculaire (IRIBHM), University of Brussels (ULB), Campus Erasme, 808 Route de Lennik, B-1070 Brussels, Belgium.
authors.forename: Fernando
authors.initials: F
authors.lastname: Mendive
authors.forename: Patrick
authors.initials: P
authors.lastname: Laurent
authors.forename: Grégory
authors.initials: G
authors.lastname: Van Schoore
authors.forename: William
authors.initials: W
authors.lastname: Skarnes
authors.forename: Roland
authors.initials: R
authors.lastname: Pochet
authors.forename: Gilbert
authors.initials: G
authors.lastname: Vassart
mesh_terms: D000483:Alleles; D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism; D001692:Biological Transport; D001835:Body Weight; D001973:Bromodeoxyuridine / Q000494:pharmacology; D049109:Cell Proliferation; D004822:Epididymis / Q000196:embryology / Q000378:metabolism; D004848:Epithelium / Q000196:embryology; D047628:Estrogen Receptor alpha / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005822:Genetic Vectors; D005837:Genitalia, Male / Q000196:embryology* / Q000473:pathology; D005838:Genotype; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D016296:Mutagenesis; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D015398:Signal Transduction; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000196:embryology / Q000378:metabolism; D014343:Trypan Blue / Q000494:pharmacology
chemical_list: D047628:Estrogen Receptor alpha; C496200:LGR4 protein, mouse; D043562:Receptors, G-Protein-Coupled; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers; D051398:Aquaporin 1; D012313:RNA; D001973:Bromodeoxyuridine; D014343:Trypan Blue