title: Flow-injection determination of L-histidine with an immobilized histidine oxidase from Brevibacillus borstelensis KAIT-B-022 and chemiluminescence detection.
abstract: A chemiluminometric flow injection analytical system for the quantitation of L-histidine is described. Histidine oxidase (EC 1.4.3.-) from Brevibacillus borstelensis KAIT-B-022 was immobilized on tresylated poly(vinyl alcohol) beads and packed into a stainless-steel column. The hydrogen peroxide produced was detected chemiluminometrically by a flowthrough sensor containing immobilized peroxidase (EC 1.1 1.1.7). The maximum sample throughput was 10 h(-1). The calibration graph was linear from 0.05 to 5 mM; the detection limit (signal to noise ratio = 3) was 0.01 mM. The activity of immobilized histidine oxidase reduced to 65% of the initial value after 350 injections. The system was applied to the determination of L-histidine in fish meat, such as salmon, tunny, bonito, and mackerel.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, University of Yamanashi, Takeda 4-3-11, Kofu 400-8511, Japan.
authors.forename: Nobutoshi
authors.initials: N
authors.lastname: Kiba
authors.forename: Ayumi
authors.initials: A
authors.lastname: Koga
authors.forename: Masaki
authors.initials: M
authors.lastname: Tachibana
authors.forename: Kazue
authors.initials: K
authors.lastname: Tani
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Koizumi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Koyama
authors.forename: Akira
authors.initials: A
authors.lastname: Yamamura
authors.forename: Kunio
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Tohru
authors.initials: T
authors.lastname: Okuda
authors.forename: Koki
authors.initials: K
authors.lastname: Yokotsuka
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis / Q000379:methods; D006639:Histidine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D010088:Oxidoreductases / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D006639:Histidine; D010088:Oxidoreductases