Pathological mechanisms of alcohol-induced hepatic portal hypertension in early stage fibrosis rat model.
OBJECTIVE
To study the role of hepatic sinusoidal capillarization and perisinusoidal fibrosis in rats with alcohol-induced portal hypertension and to discuss the pathological mechanisms of alcohol-induced hepatic portal hypertension.


METHODS
Fifty SD rats were divided into control group (n=20) and model group (n=30). Alcoholic liver fibrosis rat model was induced by intragastric infusion of a mixture containing alcohol, corn oil and pyrazole (1 000:250:3). Fifteen rats in each group were killed at wk 16. The diameter and pressure of portal vein were measured. Plasma hyaluronic acid (HA), type IV collagen (CoIV) and laminin (LN) were determined by radioimmunoassay. Liver tissue was fixed in formalin (10%) and 6-mum thick sections were routinely stained with Mallory and Sirius Red. Liver tissue was treated with rabbit polyclonal antibody against LN and ColIV. Hepatic non-parenchymal cells were isolated, total protein was extracted and separated by SDS-PAGE. MMP-2 and TIMP-1 protein expression was estimated by Western blotting.


RESULTS
The diameter (2.207+/-0.096 vs 1.528+/-0.054 mm, P<0.01) and pressure (11.014+/-0.395 vs 8.533+/-0.274 mmHg, P<0.01) of portal vein were significantly higher in model group than those in the control group. Plasma HA (129.97+/-16.10 vs 73.09+/-2.38 ng/mL, P<0.01), ColIV (210.49+/-4.36 vs 89.65+/-4.42 ng/mL, P<0.01) and LN (105.00+/-7.29 vs 55.70+/-4.32 ng/mL, P<0.01) were upregulated in model group. Abundant collagen deposited around the central vein of lobules, hepatic sinusoids and hepatocytes in model group. ColI and ColIII increased remarkably and perisinusoids were almost surrounded by ColIII. Immunohistochemical staining showed that ColIV protein level (0.130+/-0.007 vs 0.032+/-0.004, P<0.01) and LN protein level (0.152+/-0.005 vs 0.029+/-0.005, P<0.01) were up-regulated remarkably in model group. MMP-2 protein expression (2.306+/-1.089 vs 0.612+/-0.081, P<0.01) and TIMP-1 protein expression (3.015+/-1.364 vs 0.446+/-0.009, P<0.01) in freshly isolated hepatic non-parenchymal cells were up-regulated in model group and TIMP-1 protein expression was evidently higher than MMP-2 protein expression (2.669+/-0.170 vs 1.695+/-0.008, P<0.05).


CONCLUSIONS
Hepatic sinusoidal capillarization and peri-sinusoidal fibrosis are responsible for alcohol-induced portal hypertension in rats.
Li
Jian
J
Cell Biochemistry Laboratory, Basic Medicine College, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Niu
Jian-Zhao
JZ
Wang
Ji-Feng
JF
Li
Yu
Y
Tao
Xiao-Hua
XH
D000818:Animals; D004195:Disease Models, Animal; D005355:Fibrosis; D006975:Hypertension, Portal / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology*; D008102:Liver Circulation; D008108:Liver Diseases, Alcoholic / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D008833:Microcirculation; D051381:Rats; D017207:Rats, Sprague-Dawley