Expression of cystatin C in clinical human colorectal cancer tissues.
We studied the relation between the antipapain activity of cysteine proteinase inhibitors (CPI) and immunohistochemical staining for cystatin C, using anti-chicken cystatin antibodies, in the colorectal cancer tissues. In primary tumour tissues immuno-peroxidase reactivity was present in the cytoplasm and on the cell surface membranes. Sections of non malignant tissues showed no staining. The percentages of positive staining were greater for adenocarcinoma than carcinoma,100% and 77% respectively. Antipapain activity which was increased in malignant tissues in comparison to control, rose successively from well differentiated carcinomas through moderately to poor differentiated. Invasive adenocarcinomas had higher antipapain activity than noninvasive ones. The results indicated that immunohistochemical detection of cystatin using anti-chicken cystatin antibodies could be useful in studying the prognostic significance of cystatin C expression in colorectal cancer.
Saleh
Yousif
Y
Department of Forensic Medicine, Molecular Technical Unit, Wroclaw Medical University, Curie-Sklodowskiej 52, 50-369 Wroclaw, Poland. biolcancer@op.pl
Sebzda
Tadeusz
T
Warwas
Maria
M
Kopec
Wieslaw
W
Ziólkowska
Jolanta
J
Siewinski
Maciej
M
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000818:Animals; D000976:Antipain / Q000097:blood; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D002645:Chickens / Q000276:immunology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D055316:Cystatin C; D015891:Cystatins / Q000096:biosynthesis*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis
C525030:CST3 protein, human; D055316:Cystatin C; D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D000976:Antipain