Differential effects of cooked beans and cooked lentils on protein metabolism in intestine and muscle in growing rats.
OBJECTIVE
The effect of diets based on cooked beans or lentils on protein metabolism in intestines and muscles was studied in rats.


METHODS
The cooked seeds were used as the unique protein source in balanced diets (containing 229 and 190 g of crude protein per kg dry matter) fed to young growing rats for 20 days. Their effects were compared with those of the control casein diet in pair-fed rats. Protein synthesis rates in small and large intestines and in gastrocnemius and soleus muscles were determined in vivo, in a fed state, by the flooding dose method, using 13C-valine.


RESULTS
In the small and large intestine tissues of the legume fed groups, protein, RNA relative masses (mg.100 g BM(-1)) and protein synthesis rates (FSR and ASR) were higher than in the control rats (p < 0.05). In gastrocnemius and soleus muscles,protein and RNA contents (in mg) and protein synthesis rates were significantly (p < 0.05) lower in the legume-fed groups than in the control rats.


CONCLUSIONS
The chronic intake of cooked legumes increased protein synthesis rates in intestinal tissues and decreased them in muscles. This effect was greater for beans than for lentils in the large intestine and in gastrocnemius muscle.
Pirman
Tatjana
T
Chair of Nutrition, Zootechnical Department, Biotechnical Faculty, University of Ljubljana, Domzale, Slovenia. tatjana.pirman@bfro.uni-lj.si
Combe
Etiennette
E
Ribeyre
Marie Claude
MC
Prugnaud
Jacques
J
Stekar
Jasna
J
Patureau Mirand
Philippe
P
D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D002247:Carbon Isotopes; D003296:Cooking / Q000379:methods; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000378:metabolism*; D007887:Fabaceae / Q000378:metabolism*; D007420:Intestine, Large / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D027806:Lens Plant / Q000378:metabolism*; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D009928:Organ Specificity; D014176:Protein Biosynthesis / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D002247:Carbon Isotopes; D004044:Dietary Proteins; D009124:Muscle Proteins