[Periosteum construction in vitro by small intestinal submucosa combined with bone marrow mesenchymal stem cell].
OBJECTIVE
To investigate the practicability of periosteum construction in vitro by small intestinal submucosa (SIS) as a tissue scaffold with bone marrow mesenchymal stem cells (BMSC).


METHODS
The bone marrow mesenchymal stem cells were cultivated by the conventional method in vitro, and then co-cultivated with SIS. The morphological feature of the complex material, the extracellular matrices, the adhesion and proliferation of BMSC were observed by optical microscope, electronic microscope, scanning electronic microscope and histologic evaluation respectively.


RESULTS
BMSCs adhered and proliferated on SIS, secreted a great deal of extracellular matrices with active cellular function. BMSCs grew well there in layers, and the thickness became thicker as time passed, acting like a biological periosteum.


CONCLUSIONS
Combined culture of SIS and BMSC in vitro can construct a tissue engineering periosteum similar to the natural one, thus providing a base for further study of its osteogenic regeneration in vivo.
Zhang
Kai-gang
KG
Department of Orthopedic Surgery, the Sixth People's Hospital, Jiaotong University, Shanghai 200233, China.
Zeng
Bing-fang
BF
Zhang
Chang-qing
CQ
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D007413:Intestinal Mucosa / Q000033:anatomy & histology; D007421:Intestine, Small / Q000033:anatomy & histology; D008567:Membranes, Artificial; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010521:Periosteum; D011817:Rabbits; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
D008567:Membranes, Artificial