Expression of lectin-like oxidized LDL receptor-1 in smooth muscle cells after vascular injury.
Lectin-like oxidized LDL receptor-1 (LOX-1) is an oxidized LDL receptor, and its role in restenosis after angioplasty remains unknown. We used a balloon-injury model of rabbit aorta, and reverse transcription-polymerase chain reaction revealed that LOX-1 mRNA expression was modest in the non-injured aorta, reached a peak level 2 days after injury, and remained elevated until 24 weeks after injury. Immunohistochemistry and in situ hybridization showed that LOX-1 was not detected in the media of non-injured aorta but expressed in both medial and neointimal smooth muscle cells (SMC) at 2 and 24 weeks after injury. Low concentrations of ox-LDL (10 microg/mL) stimulated the cultured SMC proliferation, which was inhibited by antisense oligonucleotides of LOX-1 mRNA. Double immunofluorescence staining showed the colocalization of LOX-1 and proliferating cell nuclear antigen in human restenotic lesion. These results suggest that LOX-1 mediates ox-LDL-induced SMC proliferation and plays a role in neointimal formation after vascular injury.
Eto
Hideyuki
H
Department of Cardiovascular, Respiratory and Metabolic Medicine, Graduate School of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520, Japan.
Miyata
Masaaki
M
Kume
Noriaki
N
Minami
Manabu
M
Itabe
Hiroyuki
H
Orihara
Koji
K
Hamasaki
Shuichi
S
Biro
Sadatoshi
S
Otsuji
Yutaka
Y
Kita
Toru
T
Tei
Chuwa
C
D000818:Animals; D001011:Aorta / Q000378:metabolism; D017073:Atherectomy; D015153:Blotting, Western; D049109:Cell Proliferation; D002478:Cells, Cultured; D023903:Coronary Restenosis / Q000473:pathology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000293:injuries; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D037102:Lectins / Q000737:chemistry*; D008297:Male; D008856:Microscopy, Fluorescence; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051127:Scavenger Receptors, Class E / Q000096:biosynthesis* / Q000737:chemistry; D013997:Time Factors
D037102:Lectins; C495138:Olr1 protein, mouse; D012333:RNA, Messenger; D051127:Scavenger Receptors, Class E