title: Inhibition of intestinal glucose uptake by aporphines and secoaporphines.
abstract: The effects of aporphines and secoaporphines on glucose uptake by isolated intestinal brush-border membrane vesicles (BBMV) or basolateral membrane vesicles (BLMV) and glucose absorption during in situ intestinal perfusion were studied. Of the tested compounds, N-allylsecoboldine was the most potent glucose uptake inhibitor, with IC50 values of 159 microM and 121 microM, respectively, for uptake by BBMV and BLMV. While thaliporphine competitively inhibited glucose uptake by both membrane preparations, inhibition by N-allylsecoboldine was competitive using BBMV and noncompetitive using BLMV. In addition, N-allylsecoboldine significantly reduced both glucose absorption during in situ intestinal perfusion and blood glucose levels in the oral glucose tolerance test. The results demonstrate that levels of both aporphines and secoaporphines achievable by oral administration have an inhibitory effect on intestinal glucose uptake and suggest that the hypoglycemic effects of these compounds merit attention.
authors.affiliation: School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Lin
authors.forename: Chia-Hao
authors.initials: CH
authors.lastname: Chen
authors.forename: Fu-Wen
authors.initials: FW
authors.lastname: Liu
authors.forename: Jaw-Jou
authors.initials: JJ
authors.lastname: Kang
authors.forename: Chien-Kuang
authors.initials: CK
authors.lastname: Chen
authors.forename: Su-Lin
authors.initials: SL
authors.lastname: Lee
authors.forename: Shoei-Sheng
authors.initials: SS
authors.lastname: Lee
mesh_terms: D000465:Algorithms; D000818:Animals; D001060:Aporphines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism*; D005951:Glucose Tolerance Test; D051273:Glucose Transporter Type 2 / Q000187:drug effects / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D022142:Secretory Vesicles / Q000378:metabolism; D051269:Sodium-Glucose Transporter 1 / Q000378:metabolism
chemical_list: D001060:Aporphines; D051273:Glucose Transporter Type 2; D051269:Sodium-Glucose Transporter 1; D005947:Glucose