title: Electroporation as a tool to transfer the plasmid pRL489 in Oscillatoria MKU 277.
abstract: To establish the gene transfer system in the cyanobacteria, we successfully introduced the shuttle plasmid pRL489 into Oscillatoria MKU 277 by electroporation with transformation frequencies of up to 102 cfu/mug of plasmid DNA. These findings suggest that Oscillatoria MKU 277 can be used as an experimental tool for genetic maneuvering.
authors.affiliation: Department of Pharmacology, M.C. 7764, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA. ravindran@uthscsa.edu
authors.forename: C R Marutha
authors.initials: CR
authors.lastname: Ravindran
authors.forename: S
authors.initials: S
authors.lastname: Suguna
authors.forename: S
authors.initials: S
authors.lastname: Shanmugasundaram
mesh_terms: D000458:Cyanobacteria / Q000235:genetics*; D018274:Electroporation; D018014:Gene Transfer Techniques; D010957:Plasmids / Q000235:genetics*; D014169:Transformation, Bacterial