PCR detection of toxic shock syndrome toxin of Staphylococcus aureus from Tripoli, Libya.
Sixty-three Staphylococcus aureus strains (40 from clinical sources and 23 from food sources) were examined for toxic shock syndrome toxin-1 (TSST-1) using PCR, phage typed using the international phage set (IPS) and tested for their susceptibility to antibiotics. Only three strains (all from clinical sources) were positive for the TSST-1 gene (tst). The majority of S. aureus strains that were typeable by IPS belonged to group II. Resistance to one or more antibiotics was detected in 47.5 and 73.9 % of clinical and food strains, respectively. This is the first time that PCR detection of tst in S. aureus has been reported from Libya, and further studies are needed on the occurrence of toxic shock syndrome in the community and the role of TSST-1-producing S. aureus in this disease in Libya.
El-Ghodban
Abdulmula
A
Department of Microbiology, Faculty of Science, Etövös Loránd University, Budapest, Hungary.
Ghenghesh
Khalifa Sifaw
KS
Márialigeti
Károly
K
Department of Microbiology, Faculty of Science, Etövös Loránd University, Budapest, Hungary.
Esahli
Hamida
H
Tawil
Abdurrahman
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D001434:Bacteriophage Typing; D024881:Drug Resistance, Bacterial; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D005516:Food Microbiology; D006801:Humans; D008002:Libya; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology; D013204:Staphylococcus Phages / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D018089:Superantigens / Q000096:biosynthesis* / Q000235:genetics
D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal