title: Inhibition of trypsin-induced mast cell activation by acanthoic acid.
abstract: Acanthoic acid (AA) is a pimaradiene diterpene isolated from the Korean medicinal plant, Acanthopanax koreanum (Araliaceae). In the present study, we examined whether AA has the inhibitory effect on the production of inflammatory mediators and activating signals induced in trypsin-treated human leukemic mast cell-1 (HMC-1). HMC-1 cells were stimulated with trypsin (100 nM) in the presence or absence of AA (1, 10, and 100 microg/ml). We assessed the production of TNF-alpha and tryptase by enzyme-linked immunosorbent assay (ELISA) or reverse transcription-PCR, ERK activation by Western blot, and NF-kappaB activation by gel shift assay. AA (10 and 100 microg/ml) significantly inhibited production of both TNF-alpha and tryptase in a dose-dependent manner in trypsin-stimulated HMC-1. Furthermore, AA inhibited ERK phosphorylation and NF-kappaB activation induced by trypsin treatment without blocking of trypsin activity even with 100 microg/ml. These results suggest that AA may inhibit the production of inflammatory mediators through inhibition of ERK phosphorylation and NF-kappaB activation pathway in human mast cells. It supports the evidence that AA may be used to blocks the development of inflammation caused from mast cells.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea.
authors.forename: Ok-Hwa
authors.initials: OH
authors.lastname: Kang
authors.forename: Yeon-A
authors.initials: YA
authors.lastname: Choi
authors.forename: Hye-Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Chon-Sik
authors.initials: CS
authors.lastname: Kang
authors.forename: Bong-Seok
authors.initials: BS
authors.lastname: Song
authors.forename: Suck-Chei
authors.initials: SC
authors.lastname: Choi
authors.forename: Yong-Ho
authors.initials: YH
authors.lastname: Nah
authors.forename: Ki-Jung
authors.initials: KJ
authors.lastname: Yun
authors.forename: Xing-Fu
authors.initials: XF
authors.lastname: Cai
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Ki-Hwan
authors.initials: KH
authors.lastname: Bae
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
mesh_terms: D002478:Cells, Cultured; D004224:Diterpenes / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D008407:Mast Cells / Q000187:drug effects*; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis; D014361:Trypsin Inhibitors / Q000494:pharmacology*; D053802:Tryptases / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: D004224:Diterpenes; D016328:NF-kappa B; D012333:RNA, Messenger; D014361:Trypsin Inhibitors; D014409:Tumor Necrosis Factor-alpha; C099958:acanthoic acid; D048049:Extracellular Signal-Regulated MAP Kinases; D053802:Tryptases