title: Killing of Enterococcus faecalis by MTAD and chlorhexidine digluconate with or without cetrimide in the presence or absence of dentine powder or BSA.
abstract: The antibacterial efficacy of irrigating solutions and local disinfectants used in endodontics appears poorer in vivo than in vitro. One explanation may be inactivation by compounds present in the root canal. MTAD (a mixture of tetracycline isomer, acid, and detergent) is a new root canal irrigation solution with antibacterial activity. The aim of this study was to investigate the antibacterial activity of MTAD and chlorhexidine towards two strains of Enterococcus faecalis and the inhibitory effects of dentine and bovine serum albumin on the antibacterial activity. Survival of bacteria exposed to the medicaments in the presence or absence of inhibitors was monitored in an in vitro model. Full concentration (100%) MTAD and 0.2% chlorhexidine rapidly killed both strains. Combining chlorhexidine with cetrimide further reduced the time required for killing. The presence of dentine or BSA caused a marked delay in killing by both medicaments. The two E. faecalis strains tested showed minor differences in their susceptibility to the disinfectants.
authors.affiliation: Department of Endodontics, Dental Faculty, University of Oslo, Oslo, Norway.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Portenier
authors.forename: Tuomas
authors.initials: T
authors.lastname: Waltimo
authors.forename: Dag
authors.initials: D
authors.lastname: Ørstavik
authors.forename: Markus
authors.initials: M
authors.lastname: Haapasalo
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D019343:Citric Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003804:Dentin / Q000737:chemistry; D004318:Doxycycline / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D011136:Polysorbates / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology
chemical_list: D000891:Anti-Infective Agents, Local; D002593:Cetrimonium Compounds; D004338:Drug Combinations; C475043:MTAD (intracanal irrigant); D011136:Polysorbates; D012388:Root Canal Irrigants; D012710:Serum Albumin, Bovine; D019343:Citric Acid; C010882:chlorhexidine gluconate; D004318:Doxycycline; D002710:Chlorhexidine; D000077286:Cetrimonium