title: [Expression of integrin alpha5 and actin in the cells of intervertebral disc under cyclic hydrostatic pressure in vitro].
abstract: OBJECTIVE
  To investigate the expression of integrin alpha5 and actin in the cells of intervertebral disc under cyclic hydrostatic pressure in vitro.
  
  
  METHODS
  The porcine lumbar intervertebral disc cells were isolated and cultured in vitro, and the cells underwent cyclic hydrostatic loading. After that, the expression of integrin alpha5 and actin in intervertebral disc cells were studied by means of morphology observing, Western blot and immunohistochemistry staining.
  
  
  RESULTS
  The morphology of intervertebral disc cells were changed into smaller and flatten shape, and the expression of integrin alpha5 and actin were decreased after loading.
  
  
  CONCLUSIONS
  The expression of integrin alpha5 decreases under cyclic hydrostatic pressure, and the actin is affected at the same time when signals are transferred into the cells by integrin alpha5. That may be one of the important mechanisms of the mechanotransduction in the cells of intervertebral disc.
authors.affiliation: Department of Orthopaedics, Peking union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Sheng-ji
authors.initials: SJ
authors.lastname: Yu
authors.forename: Gui-xing
authors.initials: GX
authors.lastname: Qiu
authors.forename: Yang
authors.initials: Y
authors.lastname: Burton
authors.forename: Roth
authors.initials: R
authors.lastname: Sandra
authors.forename: Whyne
authors.initials: W
authors.lastname: Cari
authors.forename: Yee
authors.initials: Y
authors.lastname: Albert
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D006874:Hydrostatic Pressure / Q000009:adverse effects; D039482:Integrin alpha5 / Q000378:metabolism*; D007403:Intervertebral Disc / Q000166:cytology*; D040542:Mechanotransduction, Cellular; D013552:Swine
chemical_list: D000199:Actins; D039482:Integrin alpha5