Plant sulfite oxidase as novel producer of H2O2: combination of enzyme catalysis with a subsequent non-enzymatic reaction step.
Sulfite oxidase (EC 1.8.3.1) from the plant Arabidopsis thaliana is the smallest eukaryotic molybdenum enzyme consisting of a molybdenum cofactor-binding domain but lacking the heme domain that is known from vertebrate sulfite oxidase. While vertebrate sulfite oxidase is a mitochondrial enzyme with cytochrome c as the physiological electron acceptor, plant sulfite oxidase is localized in peroxisomes and does not react with cytochrome c. Here we describe results that identified oxygen as the terminal electron acceptor for plant sulfite oxidase and hydrogen peroxide as the product of this reaction in addition to sulfate. The latter finding might explain the peroxisomal localization of plant sulfite oxidase. 18O labeling experiments and the use of catalase provided evidence that plant sulfite oxidase combines its catalytic reaction with a subsequent non-enzymatic step where its reaction product hydrogen peroxide oxidizes another molecule of sulfite. In vitro, for each catalytic cycle plant SO will bring about the oxidation of two molecules of sulfite by one molecule of oxygen. In the plant, sulfite oxidase could be responsible for removing sulfite as a toxic metabolite, which might represent a means to protect the cell against excess of sulfite derived from SO2 gas in the atmosphere (acid rain) or during the decomposition of sulfur-containing amino acids. Finally we present a model for the metabolic interaction between sulfite and catalase in the peroxisome.
Hänsch
Robert
R
Department of Plant Biology, Technical University of Braunschweig, 38023 Braunschweig, Germany.
Lang
Christina
C
Riebeseel
Erik
E
Lindigkeit
Rainer
R
Gessler
Arthur
A
Rennenberg
Heinz
H
Mendel
Ralf R
RR
D015258:Acid Rain; D000603:Amino Acids, Sulfur / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000502:physiology*; D002384:Catalysis; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology; D018517:Plant Roots / Q000201:enzymology; D050876:Sulfite Oxidase / Q000737:chemistry* / Q000502:physiology*; D013447:Sulfites / Q000378:metabolism; D013458:Sulfur Dioxide / Q000378:metabolism
D015258:Acid Rain; D000603:Amino Acids, Sulfur; D029681:Arabidopsis Proteins; D013447:Sulfites; D013458:Sulfur Dioxide; D006861:Hydrogen Peroxide; D050876:Sulfite Oxidase; D010100:Oxygen