title: Differential contributions of mammalian Rad54 paralogs to recombination, DNA damage repair, and meiosis.
abstract: Homologous recombination is a versatile DNA damage repair pathway requiring Rad51 and Rad54. Here we show that a mammalian Rad54 paralog, Rad54B, displays physical and functional interactions with Rad51 and DNA that are similar to those of Rad54. While ablation of Rad54 in mouse embryonic stem (ES) cells leads to a mild reduction in homologous recombination efficiency, the absence of Rad54B has little effect. However, the absence of both Rad54 and Rad54B dramatically reduces homologous recombination efficiency. Furthermore, we show that Rad54B protects ES cells from ionizing radiation and the interstrand DNA cross-linking agent mitomycin C. Interestingly, at the ES cell level the paralogs do not display an additive or synergic interaction with respect to mitomycin C sensitivity, yet animals lacking both Rad54 and Rad54B are dramatically sensitized to mitomycin C compared to either single mutant. This suggests that the paralogs possibly function in a tissue-specific manner. Finally, we show that Rad54, but not Rad54B, is needed for a normal distribution of Rad51 on meiotic chromosomes. Thus, even though the paralogs have similar biochemical properties, genetic analysis in mice uncovered their nonoverlapping roles.
authors.affiliation: Department of Cell Biology and Genetics, Erasmus MC, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands.
authors.forename: Joanna
authors.initials: J
authors.lastname: Wesoly
authors.forename: Sheba
authors.initials: S
authors.lastname: Agarwal
authors.forename: Stefan
authors.initials: S
authors.lastname: Sigurdsson
authors.forename: Wendy
authors.initials: W
authors.lastname: Bussen
authors.forename: Stephen
authors.initials: S
authors.lastname: Van Komen
authors.forename: Jian
authors.initials: J
authors.lastname: Qin
authors.forename: Harry
authors.initials: H
authors.lastname: van Steeg
authors.forename: Jan
authors.initials: J
authors.lastname: van Benthem
authors.forename: Evelyne
authors.initials: E
authors.lastname: Wassenaar
authors.forename: Willy M
authors.initials: WM
authors.lastname: Baarends
authors.forename: Mehrnaz
authors.initials: M
authors.lastname: Ghazvini
authors.forename: Agnieszka A
authors.initials: AA
authors.lastname: Tafel
authors.forename: Helen
authors.initials: H
authors.lastname: Heath
authors.forename: Niels
authors.initials: N
authors.lastname: Galjart
authors.forename: Jeroen
authors.initials: J
authors.lastname: Essers
authors.forename: J Anton
authors.initials: JA
authors.lastname: Grootegoed
authors.forename: Norman
authors.initials: N
authors.lastname: Arnheim
authors.forename: Olga
authors.initials: O
authors.lastname: Bezzubova
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Buerstedde
authors.forename: Patrick
authors.initials: P
authors.lastname: Sung
authors.forename: Roland
authors.initials: R
authors.lastname: Kanaar
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000737:chemistry; D004249:DNA Damage; D004265:DNA Helicases / Q000235:genetics / Q000502:physiology*; D004260:DNA Repair; D004268:DNA-Binding Proteins; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D006801:Humans; D008540:Meiosis; D051379:Mice; D008817:Mice, Mutant Strains; D016685:Mitomycin / Q000494:pharmacology; D009687:Nuclear Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051135:Rad51 Recombinase / Q000032:analysis / Q000378:metabolism; D011836:Radiation Tolerance / Q000235:genetics; D011995:Recombination, Genetic; D013234:Stem Cells / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects
chemical_list: D000903:Antibiotics, Antineoplastic; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C491221:RAD54B protein, human; D016685:Mitomycin; C495153:RAD51 protein, human; D051135:Rad51 Recombinase; D004265:DNA Helicases; C103532:RAD54L protein, human; C103534:Rad54l protein, mouse