[Changes in the electromechanical activity in the course of tetanic contraction].
The functioning of excitation-contraction coupling during tetanic contraction was investigated on frog skeletal muscle. The effect of the calcium release blocker dantrolene was tested on electrically evoked twitches and tetanic contractions. It was shown that the first: developmental stage of tetanus is inhibited by dantrolene as well as a twitch contraction, and does not influenced by calcium-free medium. This substantiates it as based on "voltage dependent Ca-release" (VDCR) mechanism of activation, when depolarization directly opens the rhyanodin receptor calcium channels. The next stage: the long lasting plateau of tetanic contraction, is directly dependent on external Ca2+ entry and also inhibited by dantrolene, and therefore may be described as "calcium-induced Ca-release" (CICR) activation mechanism. It is proposed that such change in ECC mechanism taking place during tetanic contraction, can occur also in conditions of natural muscle activity, because of its rhythmical nature.
Nasledov
G A
GA
Katina
I E
IE
Zhitnikova
Iu V
IuV
D000818:Animals; D002118:Calcium / Q000502:physiology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D003620:Dantrolene / Q000494:pharmacology; D004594:Electrophysiology; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009125:Muscle Relaxants, Central / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000187:drug effects / Q000502:physiology*; D011896:Rana temporaria; D019837:Ryanodine Receptor Calcium Release Channel / Q000187:drug effects; D012519:Sarcoplasmic Reticulum / Q000502:physiology*; D019284:Thapsigargin / Q000494:pharmacology
D002121:Calcium Channel Blockers; D009125:Muscle Relaxants, Central; D019837:Ryanodine Receptor Calcium Release Channel; D019284:Thapsigargin; D000252:Calcium-Transporting ATPases; D003620:Dantrolene; D002118:Calcium