title: Zygocactus virus X-based expression vectors and formation of rod-shaped virus-like particles in plants by the expressed coat proteins of Beet necrotic yellow vein virus and Soil-borne cereal mosaic virus.
abstract: Expression vectors were constructed from 35S promoter-containing full-length cDNA clones of Zygocactus virus X (ZVX). The expression of foreign genes was driven by the ZVX coat protein (cp) subgenomic promoter. It was successful only when the variable region downstream of the conserved putative promoter region GSTTAAGTT(X(12-13))GAA was retained. Most of the ZVX cp gene, except for a short 3' part, was replaced by the corresponding sequence of the related Schlumbergera virus X (SVX) and its cp subgenomic promoter to enable encapsidation of the transcribed RNA by an SVX/ZVX hybrid cp. Vector-expressed cp of Beet necrotic yellow vein virus (BNYVV) assembled in Chenopodium quinoa, Tetragonia expansa and Beta vulgaris leaves into particles resembling true BNYVV particles. The virus produced from these constructs retained its ability to express BNYVV cp in local infections during successive passages on C. quinoa. This ability was lost, however, in the rarely occurring systemic infections.
authors.affiliation: Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, Messeweg 11, D-38104 Braunschweig, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Koenig
authors.affiliation: Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, Messeweg 11, D-38104 Braunschweig, Germany.
authors.forename: D-E
authors.initials: DE
authors.lastname: Lesemann
authors.affiliation: Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, Messeweg 11, D-38104 Braunschweig, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Loss
authors.affiliation: Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, Messeweg 11, D-38104 Braunschweig, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Engelmann
authors.affiliation: Rheinisch-Westfälische Technische Hochschule Aachen, Institut für Biologie VII, Molekulare Biotechnologie, Worringerweg 1, D-52074 Aachen, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Commandeur
authors.affiliation: Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, Messeweg 11, D-38104 Braunschweig, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Deml
authors.affiliation: Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, Messeweg 11, D-38104 Braunschweig, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Schiemann
authors.affiliation: Technical University Braunschweig, Institut für Mikrobiologie, Spielmann-Str. 7, D-38106 Braunschweig, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Aust
authors.affiliation: Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, Messeweg 11, D-38104 Braunschweig, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Burgermeister
mesh_terms: D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D017793:Luteovirus / Q000235:genetics / Q000378:metabolism*; D009029:Mosaic Viruses / Q000235:genetics / Q000378:metabolism*; D017863:Potexvirus / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D012988:Soil Microbiology; D014158:Transcription, Genetic; D014771:Virion
chemical_list: D036022:Capsid Proteins