An essential role for DeltaFosB in the nucleus accumbens in morphine action.
The transcription factor DeltaFosB is induced in the nucleus accumbens (NAc) and dorsal striatum by the repeated administration of drugs of abuse. Here, we investigated the role of DeltaFosB in the NAc in behavioral responses to opiates. We achieved overexpression of DeltaFosB by using a bitransgenic mouse line that inducibly expresses the protein in the NAc and dorsal striatum and by using viral-mediated gene transfer to specifically express the protein in the NAc. DeltaFosB overexpression in the NAc increased the sensitivity of the mice to the rewarding effects of morphine and led to exacerbated physical dependence, but also reduced their sensitivity to the analgesic effects of morphine and led to faster development of analgesic tolerance. The opioid peptide dynorphin seemed to be one target through which DeltaFosB produced this behavioral phenotype. Together, these experiments demonstrated that DeltaFosB in the NAc, partly through the repression of dynorphin expression, mediates several major features of opiate addiction.
Zachariou
Venetia
V
Department of Psychiatry and Center for Basic Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9070, USA.
Bolanos
Carlos A
CA
Selley
Dana E
DE
Theobald
David
D
Cassidy
Michael P
MP
Kelz
Max B
MB
Shaw-Lutchman
Tamara
T
Berton
Olivier
O
Sim-Selley
Laura J
LJ
Dileone
Ralph J
RJ
Kumar
Arvind
A
Nestler
Eric J
EJ
D000818:Animals; D015153:Blotting, Western; D004399:Dynorphins / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008822:Mice, Transgenic; D009020:Morphine / Q000494:pharmacology*; D009294:Narcotics / Q000494:pharmacology*; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000502:physiology; D009293:Opioid-Related Disorders / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D012201:Reward
C483819:Fosb protein, mouse; D009294:Narcotics; D016760:Proto-Oncogene Proteins c-fos; D004399:Dynorphins; D009020:Morphine