title: VP1 sequences of German porcine parvovirus isolates define two genetic lineages.
abstract: In order to evaluate the genetic variability of Porcine parvovirus (PPV), the complete capsid protein sequences (VP1/VP2) from seven recent field isolates from Germany, one isolate from the UK and one German vaccine strain were sequenced and analysed, along with two American (NADL-2 and Kresse), three Asian and 22 Brazilian partial PPV sequences retrieved from GenBank. The analysis revealed a high degree of diversity: 1.2-2.6% at the nucleotide level and 1.2-6.8% at the amino acid level. Phylogenetic analysis defined two German clusters: one formed by four German isolates and the English, Asian and American sequences; and the second, distinct cluster formed by the other three of the seven German isolates examined. The latter cluster was still observed when the 22 partial sequences (853 nt of the 3' terminus of the VP2 gene) from the Brazilian isolates were included in the analyses, indicating that the VP2 sequence determines the phylogeny.
authors.affiliation: Institut für Mikrobiologie der Bundeswehr, Neuherbergstraße 11, 80937 München, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Zimmermann
authors.affiliation: Klinik für Schweine, Tierärztliche Fakultät, Ludwig-Maximilians-Universität München, Sonnenstraße 16, 85764 Oberschleißheim, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Ritzmann
authors.affiliation: Impfstoffwerke Dessau-Tornau GmbH, PF 400214, 06855 Rosslau, Germany.
authors.forename: H-J
authors.initials: HJ
authors.lastname: Selbitz
authors.affiliation: Klinik für Schweine, Tierärztliche Fakultät, Ludwig-Maximilians-Universität München, Sonnenstraße 16, 85764 Oberschleißheim, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Heinritzi
authors.affiliation: Institut für Tierhygiene und Öffentliches Veterinärwesen, Veterinärmedizinische Fakultät Universität Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Truyen
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D014644:Genetic Variation; D005858:Germany; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections; D028323:Parvovirus, Porcine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D013552:Swine; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D015678:Viral Structural Proteins; C095547:capsid protein VP1, parvovirus B19