title: Occurrence of co-infection by Leishmania (Leishmania) chagasi and Trypanosoma (Trypanozoon) evansi in a dog in the state of Mato Grosso do Sul, Brazil.
abstract: A natural case of co-infection by Leishmania and Trypanosoma is reported in a dog (Canis familiaris) in south- western state of Mato Grosso do Sul, Brazil. Both amastigote and trypomastigote forms were observed after Giemsa staining of cytological preparations of the dog's bone marrow aspirate. No parasite was detected using medium culture inoculation of the sample. DNA obtained from the bone marrow aspirate sample and from the blood buffy coat was submitted to polymerase chain reaction (PCR) with a set of rDNA-based primers S4/S12. The nucleotide sequence of the PCR product was identical to that of Trypanosoma (Trypanozoon) evansi. The S4/S12 PCR was then used as template in a nested-PCR using a specific Leishmania set S17/S18 as primers, to explain the amastigote forms. The nucleotide sequence of the new PCR product was identical to that of Leishmania (Leishmania) chagasi. This case, as far as we know, is the first report of a dog co-infected with these parasites, suggesting that besides L. (L.) chagasi, the natural transmission of T. (T.) evansi occurs in the area under study.
authors.affiliation: Laboratório de Zoonoses e Doenças Transmitidas por Vetores da Vigilância em Saúde, Prefeitura do Município de São Paulo, 02031-020 São Paulo, SP, Brazil. elisasanmar@yahoo.com.br
authors.forename: Elisa San Martin Mouriz
authors.initials: ES
authors.lastname: Savani
authors.forename: Vania Lúcia Brandão
authors.initials: VL
authors.lastname: Nunes
authors.forename: Eunice Aparecida Bianchi
authors.initials: EA
authors.lastname: Galati
authors.forename: Tiago Moreno
authors.initials: TM
authors.lastname: Castilho
authors.forename: Fernando Shiroma de
authors.initials: FS
authors.lastname: Araujo
authors.forename: Iêda Maria Novaes
authors.initials: IM
authors.lastname: Ilha
authors.forename: Maria Cecília Gibrail de Oliveira
authors.initials: MC
authors.lastname: Camargo
authors.forename: Sandra Regina Nicoletti
authors.initials: SR
authors.lastname: D'Auria
authors.forename: Lucile Maria
authors.initials: LM
authors.lastname: Floeter-Winter
mesh_terms: D000818:Animals; D001938:Brazil; D016054:DNA, Protozoan / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D018314:Leishmania infantum / Q000235:genetics / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D014345:Trypanosoma / Q000235:genetics / Q000276:immunology; D014352:Trypanosomiasis / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal