Synthetic pyrethroid cypermethrin induced cellular damage in reproductive tissues of Drosophila melanogaster: Hsp70 as a marker of cellular damage.
We tested a working hypothesis of whether the synthetic pyrethroid cypermethrin, used worldwide for insecticidal purpose, causes adverse effects on reproduction in Drosophila melanogaster. Freshly eclosed first instar larvae of a transgenic strain of Drosophila melanogaster, Bg9, transgenic for hsp70 (hsp70-lacZ), were transferred to different dietary concentrations of the test chemical (0.002, 0.02, 0.2, 0.5, and 50.0 ppm). Larval mortality was observed at the higher dosed groups (0.2, 0.5, and 50.0 ppm). Following pair mating of virgin flies emerging from the treatment groups, a significant (p<0.05) effect on reproduction was observed in the lowest two dietary concentrations of the test chemical as compared to control. The test chemical exhibited a hazardous effect on the reproductive organs of the exposed organism as evident by Hsp70 expression and tissue damage. The impact of damage was comparatively more prominent in male flies than in females. Hsp70 expression was restricted only within the testis lobes of male, while ovary in the female fly did not exhibit any Hsp70 expression. Interestingly, the accessory glands of male flies in these treatment groups reflected intense tissue damage as evident by Trypan Blue staining. This was further corroborated by ultrastructural changes like higher vacuolization and disorganized filamentous bodies in the accessory glands of these groups. The present study indicates a profound effect on reproduction by cypermethrin and suggests the protective role of hsp70.
Mukhopadhyay
Indranil
I
Embryotoxicology Section, Industrial Toxicology Research Centre, P.O. Box No. 80, M.G. Marg, Lucknow, 226 001, Uttar Pradesh, India.
Siddique
Hifzur Rahman
HR
Bajpai
Virendra Kumar
VK
Saxena
Daya Krishna
DK
Chowdhuri
Debapratim Kar
DK
D000818:Animals; D015415:Biomarkers; D004331:Drosophila melanogaster; D005260:Female; D005836:Genitalia, Female / Q000187:drug effects* / Q000473:pathology; D005837:Genitalia, Male / Q000187:drug effects* / Q000473:pathology; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis* / Q000502:physiology; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D008854:Microscopy, Electron; D011722:Pyrethrins / Q000633:toxicity*
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D007306:Insecticides; D011722:Pyrethrins; C017160:cypermethrin