Stenothermobacter spongiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a marine sponge in the Bahamas, and emended description of Nonlabens tegetincola.
A bacterial strain, UST030701-156T, was isolated from a marine sponge in the Bahamas. Strain UST030701-156T was orange-pigmented, Gram-negative, rod-shaped with tapered ends, slowly motile by gliding and strictly aerobic. The predominant fatty acids were a15 : 0, i15 : 0, i15 : 0 3-OH, i17 : 0 3-OH, i17 : 1omega9c and summed feature 3, comprising i15 : 0 2-OH and/or 16 : 1omega7c. MK-6 was the only respiratory quinone. Flexirubin-type pigments were not produced. Phylogenetic analysis based on 16S rRNA gene sequences placed UST030701-156T within a distinct lineage in the family Flavobacteriaceae, with 93.3 % sequence similarity to the nearest neighbour, Nonlabens tegetincola. The DNA G+C content of UST030701-156T was 41.0 mol% and was much higher than that of N. tegetincola (33.6 mol%). Strain UST030701-156T can be distinguished from other members of the Flavobacteriaceae by means of a number of chemotaxonomic and phenotypic characteristics. It is proposed, therefore, that UST030701-156T represents a novel taxon designated Stenothermobacter spongiae gen. nov., sp. nov. The type strain is UST030701-156T (= NRRL B-41138T = JCM 13191T). Carbon-source utilization by N. tegetincola was re-examined and an emended description is therefore included.
Lau
Stanley C K
SC
Coastal Marine Laboratory/Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR.
Tsoi
Mandy M Y
MM
Li
Xiancui
X
Plakhotnikova
Ioulia
I
Dobretsov
Sergey
S
Wu
Madeline
M
Wong
Po-Keung
PK
Pawlik
Joseph R
JR
Qian
Pei-Yuan
PY
D000332:Aerobiosis; D000818:Animals; D001444:Bahamas; D001482:Base Composition; D002244:Carbon / Q000378:metabolism; D005227:Fatty Acids; D041965:Flavobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D011161:Porifera / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D024482:Vitamin K 2 / Q000031:analogs & derivatives
D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6; D002244:Carbon