Proteomic analysis of pathogenic bacterium Vibrio vulnificus.
In this study we have constructed a proteome reference map of the pathogenic bacterium Vibrio vulnificus. From the reference map, we identified several virulence-related proteins, such as ToxR and ToxS, as well as numerous proteins involved in diverse cellular functions. To search for additional virulence-related proteins, we compared the whole proteomes from the wild-type and toxR mutant of V. vulnificus and found that several proteins were up- or down-regulated in the toxR mutant. We suggest that these differentially regulated proteins whose expression is coordinately controlled by a virulence regulator ToxR, some of which are already implicated in virulence, play roles in the pathogenesis of V. vulnificus.
Lee
Ah Young
AY
Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong-gu, Daejeon, Korea.
Park
Sung Goo
SG
Jang
Mi
M
Cho
Sayeon
S
Myung
Pyung Keun
PK
Kim
Young Ran
YR
Rhee
Joon Haeng
JH
Lee
Do Hee
DH
Park
Byoung Chul
BC
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014157:Transcription Factors / Q000235:genetics / Q000494:pharmacology*; D041261:Vibrio vulnificus / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence
D001426:Bacterial Proteins; D015415:Biomarkers; D004268:DNA-Binding Proteins; D020543:Proteome; D014157:Transcription Factors; C066719:toxR protein, bacteria