Immunohistochemical analysis of the biological potential of odontogenic keratocysts.
BACKGROUND
The aim of this study was to analyse the usefulness of detecting important apoptosis and proliferation markers in assessing the biological potential of odontogenic keratocysts (OKC) and thus selecting the optimal diagnostic algorithm for these lesions.


METHODS
Indirect immunohistochemistry and relevant statistical methods were used for analysis of formalin-fixed and paraffin-embedded samples from 98 patients.


RESULTS
Nevoid basal cell carcinoma syndrome (NBCCS) keratocysts were characterized by higher expression of Bcl-2, p27Kip1 and c-erbB-2 as well as by lower proliferative activity measured by Ki-67 in basal cell epithelium and by a lower inflammatory response in comparison with sporadic keratocysts. Dentigerous, radicular and non-specified odontogenic cysts differed from both NBCCS and sporadic keratocysts in a wide spectrum of apoptosis and/or cell cycle-related protein expressions, higher proliferation in the basal cell layer, and vice versa, lower proliferation in the suprabasal cell layer.


CONCLUSIONS
The NBCCS keratocysts have a different immunophenotype from sporadic keratocysts and both types are distinguishable from dentigerous, radicular and non-specified odontogenic cysts. These findings confirm the separate biological potential of these lesions and the results of the immunohistochemical analysis have diagnostic and prognostic implications.
Kolár
Zdenek
Z
Institute of Pathology and Laboratory of Molecular Pathology, Faculty of Medicine, Palacký University, Olomouc, Czech Republic. kolarz@tunw.upol.cz
Geierová
Marie
M
Bouchal
Jan
J
Pazdera
Jindrich
J
Zboril
Vítezslav
V
Tvrdý
Peter
P
D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000032:analysis; D001478:Basal Cell Nevus Syndrome / Q000378:metabolism / Q000473:pathology; D001695:Biology; D015415:Biomarkers / Q000032:analysis; D018797:Cell Cycle Proteins / Q000032:analysis; D049109:Cell Proliferation; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000032:analysis; D003803:Dentigerous Cyst / Q000737:chemistry / Q000473:pathology; D003937:Diagnosis, Differential; D004848:Epithelium / Q000737:chemistry / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D019394:Ki-67 Antigen / Q000032:analysis; D009807:Odontogenic Cysts / Q000737:chemistry / Q000473:pathology*; D011379:Prognosis; D047428:Protein Kinase Inhibitors / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D011842:Radicular Cyst / Q000737:chemistry / Q000473:pathology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis
D051017:Apoptosis Regulatory Proteins; D015415:Biomarkers; D018797:Cell Cycle Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D019394:Ki-67 Antigen; D047428:Protein Kinase Inhibitors; D019253:Proto-Oncogene Proteins c-bcl-2; D018719:Erb-b2 Receptor Tyrosine Kinases