Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil.
The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.
Araújo
Cristina Pires de
CP
Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, SP, Brazil.
Leite
Clarice Queico Fugimura
CQ
Prince
Karina Andrade de
KA
Jorge
Klaudia dos Santos Gonçalves
Kdos S
Osório
Ana Luiza Alves Rosa
AL
D000003:Abattoirs; D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D001938:Brazil; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D008460:Meat / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014380:Tuberculosis, Bovine / Q000382:microbiology*
D004269:DNA, Bacterial