title: Real-time sizing of nanoparticles in microfluidic channels using confocal correlation spectroscopy.
abstract: This Communication reports real-time sizing of nanoparticles in microfluidic systems using confocal correlation spectroscopy (CCS). CCS can be used to measure the size of both fluorescent and nonfluorescent particles at low concentrations (<nanomolar) and in small volumes (<femtoliters). With advances in nonfluorescent modes of single-particle and single-molecule detection, we anticipate CCS will find broad use in the real-time measurements of nanoparticles and macromolecules.
authors.affiliation: Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Kuyper
authors.forename: Kristi L
authors.initials: KL
authors.lastname: Budzinski
authors.forename: Robert M
authors.initials: RM
authors.lastname: Lorenz
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Chiu
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes