title: Changes in composition and content of mycolic acids in glutamate-overproducing Corynebacterium glutamicum.
abstract: Overproduction of glutamate by Corynebacterium glutamicum is induced by biotin limitation or by the supplementation of specific detergents, sublethal amounts of penicillin, or cerulenin. But, it remains unclear why these different treatments, which have different sites of primary action, produce similar effects. In this study, it was found that the cellular content of mycolic acids--characteristic constituents of Corynebacterineae that are synthesized from fatty acids and form a cell surface layer--decreased under all conditions that induced glutamate overproduction. Furthermore, short mycolic acids increased under conditions of biotin limitation and cerulenin supplementation. These results suggest that different treatments produce the same effect that causes defects in the mycolic acid layer. This is perhaps one of the key factors in overproduction of glutamate by C. glutamicum.
authors.affiliation: Department of Environmental Materials Science, Tokyo Denki University, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Kouki
authors.initials: K
authors.lastname: Akazawa
authors.forename: Jun
authors.initials: J
authors.lastname: Nakamura
authors.forename: Youko
authors.initials: Y
authors.lastname: Asakura
authors.forename: Takuji
authors.initials: T
authors.lastname: Kudo
authors.forename: Eiji
authors.initials: E
authors.lastname: Sakuradani
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakamatsu
mesh_terms: D002569:Cerulenin / Q000494:pharmacology; D002849:Chromatography, Gas; D048230:Corynebacterium glutamicum / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D004952:Esters / Q000737:chemistry; D005971:Glutamates / Q000096:biosynthesis*; D013058:Mass Spectrometry; D008745:Methylation; D015394:Molecular Structure; D009171:Mycolic Acids / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D004952:Esters; D005971:Glutamates; D009171:Mycolic Acids; D002569:Cerulenin