title: Killer immunoglobulin-like receptor and human leukocyte antigen expression as immunodiagnostic parameters for pelvic endometriosis.
abstract: OBJECTIVE
  We investigated host immunologic responses to endometriosis by comparing immune cell surface antigens in peripheral blood (PB) and peritoneal fluid (PF) from women with endometriosis with those in PB and PF from other patients.
  
  
  METHODS
  Japanese women with endometriosis (n = 56) were compared with controls with other laparoscopic diagnoses (n = 68). PB and PF were collected at the time of laparoscopy for flow cytometry.
  
  
  RESULTS
  No significant difference in phenotypic parameters of T cells (CD3, CD4, and CD8), B cells (CD19), natural killer (NK) cells (CD56), or monocytes/macrophages (CD14) was seen between women with and without endometriosis. However, increased killer immunoglobulin-like receptor (CD158a) expression by NK cells and decreased human leukocyte antigen (HLA)-ABC and -DR expression by macrophages, all suggesting decreased functional activation were found in endometriosis. These markers showed significant association with endometriosis by odds ratio, logistic regression, and decision tree analyses.
  
  
  CONCLUSIONS
  Increased CD158a(+) NK cells in PB and PF indicated decreased NK cell cytotoxicity in endometriosis, while decreased HLA expression on PF macrophages suggested impaired antigen presentation. Thus, aberrant immune responses by NK cells and macrophages may represent risk factors for endometriosis.
authors.affiliation: Department of Biology, Jiamusi University, HeiLong Jiang, China.
authors.forename: Chunbin
authors.initials: C
authors.lastname: Zhang
authors.forename: Nagamasa
authors.initials: N
authors.lastname: Maeda
authors.forename: Chiaki
authors.initials: C
authors.lastname: Izumiya
authors.forename: Yorito
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Kusume
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Oguri
authors.forename: Chika
authors.initials: C
authors.lastname: Yamashita
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Nishimori
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Hayashi
authors.forename: Jiabin
authors.initials: J
authors.lastname: Luo
authors.forename: Takao
authors.initials: T
authors.lastname: Fukaya
mesh_terms: D015415:Biomarkers; D016022:Case-Control Studies; D004198:Disease Susceptibility; D004715:Endometriosis / Q000175:diagnosis*; D005260:Female; D006680:HLA Antigens / Q000378:metabolism*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D010388:Pelvis; D011971:Receptors, Immunologic / Q000378:metabolism*; D054340:Receptors, KIR; D054342:Receptors, KIR2DL1
chemical_list: D015415:Biomarkers; D006680:HLA Antigens; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054342:Receptors, KIR2DL1