title: Characterization of polyclonal antibodies raised against a linear peptide determinant of desmoglein-3.
abstract: Our labs are pursueing the goal of exactly defining the qualities of peptide antigenicity, immunogenicity and pathogenicity in order to develop safe specific immunotherapies by utilizing specific portions of disease-associated-proteins (DAPs). Using as a model the Pemphigus vulgaris antigen (PVA) desmoglein 3 (Dsg3), we have studied the murine humoral response against the Dsg3 amino acid 49-60 peptide sequence, previously characterized as sequence having low similarity to the mouse proteome. The results show that the low-similarity Dsg3(49-60)REWVKFAKPCRE peptide does not elicit pathogenic antibodies.
authors.affiliation: Department of Internal Medicine, Immunology and Infectious Diseases, Dermatology Section, Faculty of Medicine, University of Bari, P.za G. Cesare 11, 70124 Bari, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Angelini
authors.forename: Domenico
authors.initials: D
authors.lastname: Bonamonte
authors.forename: Mong-Shang
authors.initials: MS
authors.lastname: Lin
authors.forename: Alberta
authors.initials: A
authors.lastname: Lucchese
authors.forename: Abraham
authors.initials: A
authors.lastname: Mittelman
authors.forename: Rosario
authors.initials: R
authors.lastname: Serpico
authors.forename: Simone
authors.initials: S
authors.lastname: Simone
authors.forename: Animesh A
authors.initials: AA
authors.lastname: Sinha
authors.forename: Darja
authors.initials: D
authors.lastname: Kanduc
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology*; D051182:Desmogleins / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D010257:Paraneoplastic Syndromes / Q000276:immunology / Q000473:pathology; D010392:Pemphigus / Q000276:immunology* / Q000473:pathology; D012867:Skin / Q000473:pathology
chemical_list: D000906:Antibodies; D051182:Desmogleins