Suppression of glucosylceramide synthase by RNA interference reverses multidrug resistance in human breast cancer cells.
Glucosylceramide synthase (GCS), the enzyme that converts ceramide to glucosylceramide, induce multidrug resistance (MDR) in cancer cells. Recently, RNA interference (RNAi) is a powerful strategy for gene therapy by introducing double-stranded RNA and leading to the sequence-specific destruction. We have designed two different short hairpin RNAs (shRNAs) targeting GCS and introduced them into adriamycin- resistant human breast cancer cells (MCF-7/AdrR cells) to inhibit GCS expression. The results demonstrated that the shRNAs targeting GCS decreased GCS mRNA, abolished GCS protein levels and restored the sensitivity of MCF-7/AdrR cells to several antineoplastic drugs. This study revealed that this approach can reverse MDR effectively and it may be applicable to cancer patients as a specific means to restore the sensitivity to chemotherapy.
Sun
Y-L
YL
Department of Pathology, School of Medicine, Shandong University, 250012 Jinan, China. yanlinsunny@yahoo.com.cn
Zhou
G-Y
GY
Li
K-N
KN
Gao
P
P
Zhang
Q-H
QH
Zhen
J-H
JH
Bai
Y-H
YH
Zhang
X-F
XF
D000970:Antineoplastic Agents / Q000627:therapeutic use; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000188:drug therapy / Q000201:enzymology*; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple / Q000502:physiology*; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D005260:Female; D020868:Gene Silencing; D015316:Genetic Therapy; D005964:Glucosyltransferases / Q000378:metabolism*; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D000970:Antineoplastic Agents; D012333:RNA, Messenger; D005964:Glucosyltransferases; C023246:ceramide glucosyltransferase