Patients with congenital hypothyroidism demonstrate different altered expression of plasma fibrinogen and haptoglobin polypeptide chains.
OBJECTIVE
To compare the plasma protein profiles of treated and untreated congenital hypothyroidism (CH) patients with those of normal control infants.


METHODS
Plasma samples were subjected to two-dimensional gel electrophoresis and silver staining or lectin detection. Resolved protein spots were analyzed by using computerized densitometry software.


RESULTS
The significant enhanced expression of the fibrinogen gamma-chain and reduced expression of haptoglobin beta-chain were demonstrated in the profiles of untreated CH patients using both silver staining and lectin detection methods. Lectin detection resolved only a single cluster of the haptoglobin beta-chain for the untreated patients, in contrast to five isoform clusters detected in the controls' and treated CH patients' profiles.


CONCLUSIONS
Plasma from untreated CH patients demonstrated different altered expression of fibrinogen and haptoglobin polypeptide chains, which was normalized when patients were treated. Our data also suggest differences in structures of the N-glycans of haptoglobin beta-chain of the untreated CH patients.
Yong
Phaik-Har
PH
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Junit
Sarni M
SM
Harun
Fatimah
F
Hashim
Onn H
OH
D002648:Child; D002675:Child, Preschool; D003409:Congenital Hypothyroidism / Q000097:blood* / Q000188:drug therapy; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D005340:Fibrinogen / Q000378:metabolism*; D006031:Glycosylation; D006242:Haptoglobins / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D037102:Lectins / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D012834:Silver; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013974:Thyroxine / Q000627:therapeutic use*
D006242:Haptoglobins; D037102:Lectins; D010455:Peptides; D012834:Silver; D005340:Fibrinogen; D013974:Thyroxine