title: Study on the antiinflammatory activity of methanol extract from seagrass Zostera japonica.
abstract: Methanolic extracts from the seagrass Zostera japonica were extracted successively using n-hexane (n-C(6)H(14)), dichloromethane (CH(2)Cl(2)), ethyl acetate (EtOAc), and water to give the n-C(6)H(14) (16.8%), CH(2)Cl(2) (40.6%), EtOAc (34.1%), and H(2)O (8.5%) soluble fractions, respectively. We have demonstrated that the hexane fraction has the highest capacity to inhibit proIL-1beta expression as compared to other fractions in lipopolysaccharides (LPS)-stimulated J774A.1 murine macrophages. Further analysis of the composition and antiinflammatory activity of the subfraction H5 from hexane fraction showed that it had the best antiinflammatory capacity and that it's major constituents were fatty acids, including palmitic acid methyl ester (21.5%), palmitic acid (24.02%), linoleic acid methyl ester (13.09%), oleic acid methyl ester (8.41%), and linoleic acid (7.93%), respectively. H5 inhibited LPS-induced TNFalpha, IL-1beta, and IL-6 in a dose-dependent manner, suggesting that H5 is bioactive in antiinflammation in vitro. This study is the first to report the antiinflammatory activity of extracts obtained from the seagrass Z. japonica.
authors.affiliation: Faculty of Biotechnology and Laboratory Science in Medicine, Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei 112, Taiwan.
authors.forename: Kuo-Feng
authors.initials: KF
authors.lastname: Hua
authors.forename: Hsien-Yeh
authors.initials: HY
authors.lastname: Hsu
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Su
authors.forename: I-Fan
authors.initials: IF
authors.lastname: Lin
authors.forename: Su-Sing
authors.initials: SS
authors.lastname: Yang
authors.forename: Ya-Mai
authors.initials: YM
authors.lastname: Chen
authors.forename: Louis Kuoping
authors.initials: LK
authors.lastname: Chao
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002460:Cell Line; D008401:Gas Chromatography-Mass Spectrometry; D006586:Hexanes; D007375:Interleukin-1 / Q000032:analysis / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D000432:Methanol; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D035962:Zosteraceae / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D006586:Hexanes; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000432:Methanol