Eu3+-doped Gd2O3 nanoparticles as reporters for optical detection and visualization of antibodies patterned by microcontact printing.
Lanthanide oxide nanoparticles are promising luminescent probes in bioanalysis, because of their unique spectral properties, photostability, and low-cost synthesis. We report for the first time the application of europium-doped gadolinium oxide (Eu:Gd2O3) nanoparticles to the optical imaging of antibody micropatterns. The nanoparticles were synthesized by spray pyrolysis and coated with antibody (IgG) molecules by physical adsorption. Our experiments showed that the Eu:Gd2O3 is a good biocompatible solid support for antibody immobilization. The antibodies (anti-rabbit IgG) immobilized on the nanoparticles had excellent biological activity in the specific recognition reaction with rabbit IgG patterned in line strips (10 micromx10 microm) on a glass substrate by use of a micro-contact printing technique. The specific immunoreaction was confirmed by two independent microscopic techniques-fluorescence and scanning electron microscopy (SEM). Both microscopic images revealed that the nanoparticles were organized into designated structures as defined by the microcontact printing process with negligible non-specific binding. The nanoparticles can be used as fluorescent markers in a variety of immunosensing applications in a microscale format.
Nichkova
Mikaela
M
Department of Entomology, University of California Davis, One Shields Avenue, Davis, CA 95616, USA. mnichkova@ucdavis.edu
Dosev
Dosi
D
Perron
Richard
R
Gee
Shirley J
SJ
Hammock
Bruce D
BD
Kennedy
Ian M
IM
D000327:Adsorption; D000906:Antibodies / Q000032:analysis / Q000737:chemistry*; D005063:Europium / Q000737:chemistry*; D005682:Gadolinium / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D055095:Optics and Photonics; D012680:Sensitivity and Specificity; D013499:Surface Properties
D000906:Antibodies; D005063:Europium; C030581:gadolinium oxide; D005682:Gadolinium