Characterization of polyclonal antibodies raised against a linear peptide determinant of desmoglein-3.
Our labs are pursueing the goal of exactly defining the qualities of peptide antigenicity, immunogenicity and pathogenicity in order to develop safe specific immunotherapies by utilizing specific portions of disease-associated-proteins (DAPs). Using as a model the Pemphigus vulgaris antigen (PVA) desmoglein 3 (Dsg3), we have studied the murine humoral response against the Dsg3 amino acid 49-60 peptide sequence, previously characterized as sequence having low similarity to the mouse proteome. The results show that the low-similarity Dsg3(49-60)REWVKFAKPCRE peptide does not elicit pathogenic antibodies.
Angelini
Giovanni
G
Department of Internal Medicine, Immunology and Infectious Diseases, Dermatology Section, Faculty of Medicine, University of Bari, P.za G. Cesare 11, 70124 Bari, Italy.
Bonamonte
Domenico
D
Lin
Mong-Shang
MS
Lucchese
Alberta
A
Mittelman
Abraham
A
Serpico
Rosario
R
Simone
Simone
S
Sinha
Animesh A
AA
Kanduc
Darja
D
D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology*; D051182:Desmogleins / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D010257:Paraneoplastic Syndromes / Q000276:immunology / Q000473:pathology; D010392:Pemphigus / Q000276:immunology* / Q000473:pathology; D012867:Skin / Q000473:pathology
D000906:Antibodies; D051182:Desmogleins