title: Structures of the core oligosaccharide and O-units in the R- and SR-type lipopolysaccharides of reference strains of Pseudomonas aeruginosa O-serogroups.
abstract: Highly phosphorylated core oligosaccharides and those substituted with one O-antigen repeating unit were obtained by mild acid degradation or strong alkaline hydrolysis of lipopolysaccharide samples from 23 reference strains representing all Pseudomonas aeruginosa O-serogroups. Studies by high-resolution electrospray ionization mass spectrometry and two-dimensional NMR spectroscopy revealed both conserved and variable structural features of the lipopolysaccharides of various O-serogroups. The upstream terminal saccharide of the O-antigen, which contributes most to the immunospecificity of the bacteria, was defined in 11 from a total of 13 O-serogroups. The data obtained link together the known biosynthesis pathways, genetics and serology of the P. aeruginosa lipopolysaccharide.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. olgabv@ioc.ac.ru
authors.forename: Olga V
authors.initials: OV
authors.lastname: Bystrova
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Buko
authors.initials: B
authors.lastname: Lindner
authors.forename: Nina A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: Anna N
authors.initials: AN
authors.lastname: Kondakova
authors.forename: Ulrich
authors.initials: U
authors.lastname: Zähringer
authors.forename: Gerald B
authors.initials: GB
authors.lastname: Pier
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides