Involvement of intestinal alkaline phosphatase in serum apolipoprotein B-48 level and its association with ABO and secretor blood group types.
Serum levels of intestinal alkaline phosphatase (IAP), a protein implicated in transcellular transport of chylomicrons, vary among ABO blood groups. In rat enterocytes, IAP is associated with chylomicron secretion, but the rat expresses only blood group A. It is not known whether chylomicron secretion may be affected in humans who express multiple blood group types. Serum samples from 40 healthy subjects were obtained after overnight fast and 3h after a high-fat meal, and assayed for IAP and apolipoprotein B-48 (apoB-48), both proteins exclusive to intestine, although only apoB-48 is found in chylomicrons. The two proteins were greater in subjects without blood antigen A (B and O) than in those with this antigen (A and AB); 2.4- and 4.7-fold for IAP and 1.5- and 2.0-fold for apoB-48 before and after the meal, respectively. Moreover, IAP and apoB-48 levels were strongly correlated in the subjects with the secretor phenotype (r > 0.81). These results indicate that IAP is strongly involved in chylomicron formation and fatty acid metabolism might change among ABO blood type. In addition, ABO blood type classification in apoB-48 measurement would improve the diagnostic value in the evaluation of metabolic syndrome.
Nakano
Takanari
T
Department of Biochemistry, Saitama Medical School, Japan.
Shimanuki
Tomoko
T
Matsushita
Makoto
M
Koyama
Iwao
I
Inoue
Ikuo
I
Katayama
Shigehiro
S
Alpers
David H
DH
Komoda
Tsugikazu
T
D000017:ABO Blood-Group System / Q000097:blood*; D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood*; D000951:Antigens, Neoplasm / Q000097:blood*; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B / Q000097:blood*; D004041:Dietary Fats / Q000378:metabolism*; D005260:Female; D058851:GPI-Linked Proteins; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007983:Lewis Blood Group Antigens / Q000097:blood*; D008297:Male; D013223:Statistics as Topic
D000017:ABO Blood-Group System; D000951:Antigens, Neoplasm; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D004041:Dietary Fats; D058851:GPI-Linked Proteins; D007983:Lewis Blood Group Antigens; C063088:ALPI protein, human; D000469:Alkaline Phosphatase