title: Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H+ -ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe cichoracearum.
abstract: Little is known at the molecular level about the transporters involved in nutrient transfer in the plant/powdery mildew interaction. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for an isoform of the plasma membrane H+ -ATPase (EcPMA1) in the biotrophic pathogenic fungus Erysiphe cichoracearum. Southern analysis suggests that EcPMA1 exists as a single-copy gene. Sequence analysis indicated a high similarity of EcPMA1 to other fungal H+ -ATPases. Expression of EcPMA1 increases in infected Arabidopsis leaves as the disease progresses, correlating with the growth of the pathogen.
authors.affiliation: School of Biological Sciences, University of Southampton, Biomedical Sciences Building, Bassett Crescent East, Southampton SO16 7PX, UK.
authors.forename: Vasileios
authors.initials: V
authors.lastname: Fotopoulos
authors.forename: Robert
authors.initials: R
authors.lastname: Holmes
authors.forename: J L
authors.initials: JL
authors.lastname: Hall
authors.forename: Lorraine E
authors.initials: LE
authors.lastname: Williams
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D002462:Cell Membrane; D003001:Cloning, Molecular; D015870:Gene Expression; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D006180:Proton-Translocating ATPases