title: Pro-atherogenic properties of lipopolysaccharide from the periodontal pathogen Actinobacillus actinomycetemcomitans.
abstract: An association between cardiovascular and periodontal disease may be due to lipopolysaccharide (LPS)-promoted release of inflammatory mediators, adverse alterations of the lipoprotein profile, and an imbalance in cholesterol homeostasis. Since periodontopathogenic potential differs between serotypes of a major periodontal pathogen, Actinobacillus actinomycetemcomitans, we studied the pro-atherogenic properties of LPS preparations from serotypes b and d strains on macrophages (RAW 264.7). A. actinomycetemcomitans LPS preparations induced a time-dependent release of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta). LPS induced foam cell formation and cholesteryl ester accumulation from native low density lipoprotein in the following order: A. actinomycetemcomitans strains JP2 (serotype b) > Y4 (serotype b) > IDH781 (serotype d). mRNA expression levels of scavenger receptor class B, type-I, and ATP-binding cassette transporter-1, receptors mediating cholesterol efflux from macrophages, were decreased by LPS preparations. The results suggest that the pro-atherogenic potential of A. actinomycetemcomitans LPS may depend on the infecting strain and correlate with the periodontopathogenic potential of the pathogen.
authors.affiliation: Institute of Dentistry, University of Helsinki, and Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital, Helsinki, Finland.
authors.forename: Laura
authors.initials: L
authors.lastname: Lakio
authors.forename: Markku
authors.initials: M
authors.lastname: Lehto
authors.forename: Anita M
authors.initials: AM
authors.lastname: Tuomainen
authors.forename: Matti
authors.initials: M
authors.lastname: Jauhiainen
authors.forename: Ernst
authors.initials: E
authors.lastname: Malle
authors.forename: Sirkka
authors.initials: S
authors.lastname: Asikainen
authors.forename: Pirkko J
authors.initials: PJ
authors.lastname: Pussinen
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D050197:Atherosclerosis / Q000139:chemically induced*; D002460:Cell Line; D008076:Cholesterol, HDL / Q000378:metabolism; D008078:Cholesterol, LDL / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D005487:Foam Cells / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010510:Periodontal Diseases / Q000382:microbiology*; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051122:Scavenger Receptors, Class B / Q000096:biosynthesis
chemical_list: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D016207:Cytokines; D008070:Lipopolysaccharides; D051122:Scavenger Receptors, Class B; D012313:RNA