Assay and functional analysis of dynamin-like protein 1 in peroxisome division.
Recent studies have demonstrated that peroxisome division requires at least one dynamin-like protein, Vps1p, in the yeast Saccharomyces cerevisiae and DLP1 (DRP1) in mammalian cells. Although the requirement for these proteins in peroxisome division is supported by many lines of evidence, their roles in peroxisome division have yet to be identified. Given the independence of peroxisomes from other organelle systems, the peroxisome system appears to have unique attributes for studying the function of dynamin-like proteins in organelle division. Here, we present methods that have been used for studying the role of DLP1 in peroxisome biogenesis and division.
Schrader
Michael
M
Department of Cell Biology and Cell Pathology, University of Marburg, Germany.
Gould
Stephen J
SJ
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D034281:Dynamins; D020558:GTP Phosphohydrolases / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D009154:Mutation; D020675:Peroxisomes / Q000502:physiology*; D034622:RNA Interference; D051381:Rats
D008869:Microtubule-Associated Proteins; D024101:Mitochondrial Proteins; D020558:GTP Phosphohydrolases; C110768:DNM1L protein, human; D034281:Dynamins