An empirical approach for detecting nucleotide-binding sites on proteins.
Protein structure data in the PDB (Protein Data Bank) were used to construct empirical scores of nucleotide-protein interactions. A simple strategy to evaluate the spatial distribution of protein atoms around the base moieties of nucleotides was applied to categorize adenine, guanine, nicotinamide and flavin nucleotide-binding sites. In addition to the known nucleotide-binding motifs, the empirical scores detected several other features that were shared among proteins with different folds. The empirical scores were also used to predict the binding sites on protein molecules and a comprehensive test of the prediction system was performed. As a result, adenine, guanine, nicotinamide and flavin sites were detected with efficiencies of 31, 29, 32 and 40%, respectively. The predictions were judged to be successful if the predicted base with the best score was located within a 3.0 A r.m.s.d. from the known ligand positions.
Saito
Mihoko
M
Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Go
Mitiko
M
Shirai
Tsuyoshi
T
D020816:Amino Acid Motifs; D001665:Binding Sites; D003198:Computer Simulation; D030562:Databases, Protein; D008024:Ligands; D008956:Models, Chemical; D008958:Models, Molecular; D009711:Nucleotides / Q000378:metabolism*; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
D008024:Ligands; D009711:Nucleotides; D011506:Proteins