A new phenanthrene with a spirolactone from Dendrobium chrysanthum and its anti-inflammatory activities.
Investigation of phenolic patterns from the stems of Dendrobium chrysanthum by HPLC-PDA-MS has led to the isolation of a new phenanthrene derivative with a spirolactone ring, dendrochrysanene (1), that proved to suppress the mRNA level of TNF-alpha, IL8, IL10, and iNOS in murine peritoneal macrophages. The structure of 1 was characterized on the basis of various NMR (1H, 13C, 1H-1H COSY, HMQC, and HMBC), mass spectrometry, and X-ray crystal diffraction data.
Yang
Li
L
Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Zhangjiang Hi-tech Park, Shanghai 201203, PR China.
Qin
Lin-Hua
LH
Bligh
S W Annie
SW
Bashall
A
A
Zhang
Chao-Feng
CF
Zhang
Mian
M
Wang
Zheng-Tao
ZT
Xu
Luo-Shan
LS
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D018360:Crystallography, X-Ray; D031667:Dendrobium / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D007378:Interleukins / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010616:Phenanthrenes / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D013141:Spiro Compounds / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D013148:Spironolactone / Q000737:chemistry*; D048069:Tumor Necrosis Factors / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D007378:Interleukins; D010616:Phenanthrenes; D013141:Spiro Compounds; D048069:Tumor Necrosis Factors; C512279:dendrochrysanene; D013148:Spironolactone