title: Fabrication of antibody arrays using thermally responsive elastin fusion proteins.
abstract: A general method has been developed to immobilize antibodies onto an array surface by employing fusion proteins consisting of an elastin domain with tunable hydrophobic properties and an antibody-binding domain with high binding affinity and specificity for antibodies. Antibodies conjugated with the elastin fusion proteins can be directly printed on a self-assembled monolayer-modified glass slide in a functionally active orientation with a spatially defined pattern. An antibody array sensor for detection of tumor markers was fabricated to demonstrate the utility of the method. We expect that the method presented here could be a simple and universal platform to immobilize antibodies for the fabrication of a variety of antibody array sensors.
authors.affiliation: Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, USA.
authors.forename: Di
authors.initials: D
authors.lastname: Gao
authors.forename: Nicole
authors.initials: N
authors.lastname: McBean
authors.forename: Jerome S
authors.initials: JS
authors.lastname: Schultz
authors.forename: Yushan
authors.initials: Y
authors.lastname: Yan
authors.forename: Ashok
authors.initials: A
authors.lastname: Mulchandani
authors.forename: Wilfred
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry; D004549:Elastin / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D009419:Nerve Tissue Proteins / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013205:Staphylococcal Protein A / Q000737:chemistry
chemical_list: D000906:Antibodies; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C029152:G-substrate; C116593:L-protein, Peptococcus magnus; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; D004549:Elastin