title: Nosema chrysorrhoeae n. sp. (Microsporidia), isolated from browntail moth (Euproctis chrysorrhoea L.) (Lepidoptera, Lymantriidae) in Bulgaria: characterization and phylogenetic relationships.
abstract: A new microsporidian parasite Nosema chrysorrhoeae n. sp., isolated in Bulgaria from the browntail moth (Euproctis chrysorrhoea L.), is described. Its life cycle includes two sequential developmental cycles that are similar to the general developmental cycles of the Nosema-like microsporidia and are indistinguishable from those of two Nosema spp. from Lymantria dispar. The primary cycle takes place in the midgut tissues and produces binucleate primary spores. The secondary developmental cycle takes place exclusively in the silk glands and produces binucleate environmental spores. N. chrysorrhoeae is specific to the browntail moth. Phylogenetic analysis based on the ssu rRNA gene sequence places N. chrysorrhoeae in the Nosema/Vairimorpha clade, with the microsporidia from lymantriid and hymenopteran hosts. Partial sequences of the lsu rRNA gene and ITS of related species Nosema kovacevici (Purrini K., Weiser J., 1975. Natürliche Feinde des Goldafters, Euproctis chrysorrhoea L., im Gebiet von Kosovo, FSR Jugoslawien. Anzeiger fuer Schädlingskunde, Pflanzen-Umweltschutz, 48, 11-12), Nosema serbica Weiser, 1963 and Nosema sp. from Lymantria monacha was obtained and compared with N. chrysorrhoeae. The molecular data indicate the necessity of future taxonomic reevaluation of the genera Nosema and Vairimorpha.
authors.affiliation: Department of Parasitology and Laboratory of Electron Microscopy, Faculty of Science, Charles University, Prague, Czech Republic. mirekhylis@volny.cz
authors.forename: Miroslav
authors.initials: M
authors.lastname: Hylis
authors.forename: Daniela K
authors.initials: DK
authors.lastname: Pilarska
authors.forename: Miroslav
authors.initials: M
authors.lastname: Oborník
authors.forename: Jirí
authors.initials: J
authors.lastname: Vávra
authors.forename: Leellen F
authors.initials: LF
authors.lastname: Solter
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Weiser
authors.forename: Andreas
authors.initials: A
authors.lastname: Linde
authors.forename: Michael L
authors.initials: ML
authors.lastname: McManus
mesh_terms: D000818:Animals; D002031:Bulgaria; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D004847:Epithelial Cells / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D020459:Genes, rRNA / Q000235:genetics; D008018:Life Cycle Stages; D009036:Moths / Q000382:microbiology*; D009132:Muscles / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D016818:Nosema / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D010802:Phylogeny; D013045:Species Specificity
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal