Study on the effects of Guiqi Oral Liquid in promoting recovery of hematopoiesis in acute irradiation injured mice.
OBJECTIVE
To explore the effects and possible mechanisms of Guiqi Oral Liquid (GQOL) on the recovery of hematopoiesis in acute irradiation injured mice.


METHODS
The acute irradiation injured mice were randomly divided into 2 groups: the treated group and the control group, and also a normal control group was set up with 6 mice in it receiving no treatment. After the mice in the former two groups were irradiated by 6.0 Gy (60)Co gamma-ray, every one of them was given 0.4 ml GQOL or saline in equal volume through a gastric tube twice a day for 14 days. On the 4th, 8th and 14th day after irradiation, the bone marrow mononuclear cells (BMMNC) and megakaryocytes in bone marrow tissues of the mice were counted, the proportion of hematopoietic tissues (by area) was measured, and the expression of adhesion molecules, CD44 and CD54, in bone marrow were estimated by immunochemistry. The colony forming unit of spleen (CFU-S) in the mice were counted on the 8th day after irradiation.


RESULTS
On the 4th, 8th, 14th day after irradiation, the count of BMMNC and megakaryocyte, and the proportion of hematopoietic tissues in the treated group were higher than those in the control group (P < 0.01 or P < 0.05). CD44 and CD54 expression in the treated group were higher than those in the control group on the 4th and 8th day (P < 0.01), but near normal on the 14th day (P < 0.01). On the 8th day, CFU-S count in the treated group was higher than that in the control group (P < 0.01).


CONCLUSIONS
GQOL can regulate the expression of adhesion molecules, CD44 and CD54, in the bone marrow of the acute irradiation injured mice, which may be one of the mechanisms of GQOL in accelerating the early phase hematopoiesis recovery of mice.
Zheng
Miao
M
Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030. zmzk@sina.com
Liu
Wen-li
WL
Sun
Han-ying
HY
Zhou
Jian-feng
JF
D029971:Angelica sinensis; D000818:Animals; D027883:Astragalus Plant; D001854:Bone Marrow Cells / Q000166:cytology; D002452:Cell Count; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D018960:Hyaluronan Receptors / Q000032:analysis; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008533:Megakaryocytes / Q000166:cytology; D051379:Mice; D008517:Phytotherapy; D011833:Radiation Injuries, Experimental / Q000178:diet therapy* / Q000503:physiopathology; D011897:Random Allocation
D004365:Drugs, Chinese Herbal; D018960:Hyaluronan Receptors; C510022:guiqi oral liquid; D018799:Intercellular Adhesion Molecule-1