title: Axonal growth and guidance defects in Frizzled3 knock-out mice: a comparison of diffusion tensor magnetic resonance imaging, neurofilament staining, and genetically directed cell labeling.
abstract: Previous work has identified axonal outgrowth and/or guidance defects in the brain and spinal cord of prenatal Frizzled3 (Fz3)(-/-) mice. To systematically explore the axonal defects in Fz3(-/-) mice and to compare techniques for the global assessment of axon tracts in the developing mouse, we have analyzed wild-type and Fz3(-/-) brains using (1) diffusion tensor magnetic resonance imaging (muDTI), (2) neurofilament staining, and (3) two genetically directed neuronal labeling methods. Confirming and extending the previous work of Wang et al. (2002), we find that the following structures/tracts are absent or greatly reduced in the Fz3(-/-) brain: the anterior commissure, cerebral peduncle (corticospinal tract), corpus callosum, fornix, internal capsule (thalamocortical and corticothalamic tracts), stria medullaris, stria terminalis, and hippocampal commissure. An aberrant U-shaped fiber bundle immediately caudal to the optic tract connects the left and right sides of the Fz3(-/-) thalamus and likely represents a default pathway for thalamic axons that failed to enter the internal capsule. At embryonic day 18, labeling of cortical pyramidal cells with a yellow fluorescent protein reporter reveals widespread fragmentation of axons with no apparent loss of pyramidal cell bodies. Fragmentation likely represents one stage in the process that normally eliminates stalled or mistargeted axons. This work demonstrates the usefulness of muDTI and genetically directed neuronal labeling for the analysis of nervous system defects in the mouse.
authors.affiliation: Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: Yanshu
authors.initials: Y
authors.lastname: Wang
authors.forename: Jiangyang
authors.initials: J
authors.lastname: Zhang
authors.forename: Susumu
authors.initials: S
authors.lastname: Mori
authors.forename: Jeremy
authors.initials: J
authors.lastname: Nathans
mesh_terms: D000015:Abnormalities, Multiple; D000469:Alkaline Phosphatase; D000818:Animals; D001369:Axons / Q000502:physiology*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D001921:Brain / Q000002:abnormalities* / Q000648:ultrastructure; D038524:Diffusion Magnetic Resonance Imaging; D051157:Frizzled Receptors; D058851:GPI-Linked Proteins; D005804:Genes, Lethal; D017930:Genes, Reporter; D019426:Integrases / Q000378:metabolism; D007382:Intermediate Filaments / Q000737:chemistry*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009024:Morphogenesis; D009412:Nerve Fibers / Q000737:chemistry / Q000648:ultrastructure; D010641:Phenotype; D043562:Receptors, G-Protein-Coupled / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013194:Staining and Labeling / Q000379:methods*; D013623:Tail / Q000002:abnormalities
chemical_list: D001426:Bacterial Proteins; D051157:Frizzled Receptors; C486339:Fzd3 protein, mouse; D058851:GPI-Linked Proteins; D007527:Isoenzymes; D008164:Luminescent Proteins; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; C054562:yellow fluorescent protein, Bacteria; C045073:Cre recombinase; D019426:Integrases; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental