A new method of wet scanning electron microscopy for the analysis of myelination in EAE mouse model of multiple sclerosis.
Development of effective therapies for multiple sclerosis (MS) is dependent on the advancement of improved tools for evaluation of progression of this disease in animal models. We present a novel technique utilizing scanning electron microscopy (SEM) for imaging wet biological specimens thus enabling rapid and high-resolution imaging of myelin in mouse spinal cord (SC). We demonstrate the advantages of using the wet SEM technique to image myelin in a murine model of MS, experimental autoimmune encephalomyelitis (EAE) induced in the Biozzi (antibody-high) mouse, by sensitization with spinal cord homogenate (SCH) in adjuvant. Our studies show that the methodology allows easy identification of normal and pathological components with great clarity, which is then correlated with light microscopy (LM) and validated thereby. Furthermore, we demonstrate gold immunolabeling of specific epitopes. We conclude that the new technique provides a quick, accurate, and detailed structural evaluation of the SC that can be applied to advance the research of MS.
Nyska
Abraham
A
Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health, Research Triangle Park, NC, USA. anyska@bezeqint.net
Horowitz
Miri
M
Anaby
Debbie
D
Sabban
Alon
A
Leizerman
Ilit
I
Blaugrund
Eran
E
Mayk
Adi
A
Behar
Vered
V
D000818:Animals; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000473:pathology*; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009103:Multiple Sclerosis / Q000473:pathology*; D009186:Myelin Sheath / Q000648:ultrastructure*; D015203:Reproducibility of Results; D013116:Spinal Cord / Q000648:ultrastructure*