New neurons follow the flow of cerebrospinal fluid in the adult brain.
In the adult brain, neuroblasts born in the subventricular zone migrate from the walls of the lateral ventricles to the olfactory bulb. How do these cells orient over such a long distance and through complex territories? Here we show that neuroblast migration parallels cerebrospinal fluid (CSF) flow. Beating of ependymal cilia is required for normal CSF flow, concentration gradient formation of CSF guidance molecules, and directional migration of neuroblasts. Results suggest that polarized epithelial cells contribute important vectorial information for guidance of young, migrating neurons.
Sawamoto
Kazunobu
K
Department of Neurological Surgery and Developmental and Stem Cell Biology Program, University of California San Francisco, San Francisco, CA 94143, USA. sawamoto@sc.itc.keio.ac.jp
Wichterle
Hynek
H
Gonzalez-Perez
Oscar
O
Cholfin
Jeremy A
JA
Yamada
Masayuki
M
Spassky
Nathalie
N
Murcia
Noel S
NS
Garcia-Verdugo
Jose Manuel
JM
Marin
Oscar
O
Rubenstein
John L R
JL
Tessier-Lavigne
Marc
M
Okano
Hideyuki
H
Alvarez-Buylla
Arturo
A
D000818:Animals; D016380:Brain Tissue Transplantation; D002465:Cell Movement; D016764:Cell Polarity; D002552:Cerebral Ventricles / Q000166:cytology / Q000502:physiology; D002555:Cerebrospinal Fluid / Q000502:physiology*; D002831:Choroid Plexus / Q000378:metabolism; D002923:Cilia / Q000502:physiology; D004805:Ependyma / Q000166:cytology / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000134:cerebrospinal fluid; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000134:cerebrospinal fluid; D000098552:Slit Homolog 2 Protein
D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D000098552:Slit Homolog 2 Protein