Identification of target proteins of N-acetylglucosaminyl transferase V in human colon cancer and implications of protein tyrosine phosphatase kappa in enhanced cancer cell migration.
To gain a better understanding of the mechanism underlying colon cancer and to search for potential markers of colon cancer prognosis, a comparative proteomic analysis of colon cancer WiDr cells was conducted using 2-DE and lectin blot, followed by identification based on ESI-MS. Through these approaches 14 proteins were identified as candidate target proteins for N-acetylglucosaminyl transferase V (GnT-V) that would be expected to be implicated in the progression of colon cancer. We selected protein tyrosine phosphatase kappa (PTPkappa) as a model protein to validate this approach to the discovery of novel biomarkers in colon cancer. PTPkappa underwent an aberrant glycosylation in GnT-V-overexpressing WiDr cells, and the aberrantly glycosylated PTPkappa was vulnerable to proteolytic cleavage. The enhanced cleavage of PTPkappa in GnT-V-overexpressing cells was responsible for the mitigation of the homophilic binding capacity, resulting in an increase in cancer cell migration.
Kim
Yong-Sam
YS
Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, Korea.
Kang
Hye-Yeon
HY
Kim
Jin-Young
JY
Oh
Sejeong
S
Kim
Cheorl-Ho
CH
Ryu
Chun Jeih
CJ
Miyoshi
Eiji
E
Taniguchi
Naoyuki
N
Ko
Jeong Heon
JH
D002465:Cell Movement; D003110:Colonic Neoplasms / Q000201:enzymology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006031:Glycosylation; D006801:Humans; D037102:Lectins / Q000378:metabolism; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D010446:Peptide Fragments / Q000276:immunology; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2; D021241:Spectrometry, Mass, Electrospray Ionization; D014407:Tumor Cells, Cultured
D037102:Lectins; D010446:Peptide Fragments; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase; C489852:PTPRK protein, human; D017027:Protein Tyrosine Phosphatases; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2