title: Simultaneous quantitative LC-ESI-MS/MS analyses of salicylic acid and jasmonic acid in crude extracts of Cucumis sativus under biotic stress.
abstract: Salicylic acid (SA) and jasmonic acid (JA) are plant hormones involved in basal resistance against plant pathogens and also in induced resistance. The aim of this study is to develop a fast and sensitive method to determine simultaneously the levels of both these hormones. The present paper proposes a method that includes hormone extraction with MeOH-H(2)O-HOAc (90:9:1, v/v), evaporation of the extracts, and injection into the liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) system in multiple reaction monitoring (MRM). Endogenous SA and JA levels in noninfested control cucumber cotyledons were 30.96 and 0.73ngg(-1) fresh weight, respectively. In roots, the levels were 8.31 and 15.82ngg(-1) FW, respectively. In plants treated with the biological control agent Trichoderma asperellum strain T-34, the levels of SA and JA did not differ from control plants. Rhizoctonia solani-diseased cucumber plants showed higher levels of SA and JA compared to noninfested controls (up to 2 and 13-fold higher, respectively). Detection limits for SA and JA were 0.45 and 0.47ngg(-1) fresh weight, respectively. The results of our research include the development of a method that is both fast and highly sensitive in the simultaneous quantitation of SA and JA from crude cucumber plant extracts, avoiding any purification and derivatization steps.
authors.affiliation: Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Catalonia, Spain. gsegarra@ub.edu
authors.forename: Guillem
authors.initials: G
authors.lastname: Segarra
authors.forename: Olga
authors.initials: O
authors.lastname: Jáuregui
authors.forename: Eva
authors.initials: E
authors.lastname: Casanova
authors.forename: Isabel
authors.initials: I
authors.lastname: Trillas
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D018548:Cotyledon / Q000378:metabolism; D018553:Cucumis sativus / Q000737:chemistry* / Q000502:physiology; D003517:Cyclopentanes / Q000032:analysis*; D054883:Oxylipins; D010935:Plant Diseases / Q000382:microbiology; D010936:Plant Extracts / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D012232:Rhizoctonia / Q000378:metabolism; D020156:Salicylic Acid / Q000032:analysis*; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014242:Trichoderma / Q000378:metabolism
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010936:Plant Extracts; D012997:Solvents; C011006:jasmonic acid; D020156:Salicylic Acid