Transfection of CD40 in a human oral squamous cell carcinoma keratinocyte line upregulates immune potency and costimulatory molecules.
BACKGROUND
There is upregulation of class II molecules of the major histocompatibility complex (MHC) by keratinocytes in oral squamous cell carcinoma (OSCC) and inflammatory diseases such as lichen planus. The significance of this expression, or whether it is accompanied by upregulation of membrane-bound costimulatory molecules, is unknown.


OBJECTIVE
To compare the expression of CD40, CD80, CD86, MHC class II and intercellular adhesion molecule-1 (ICAM-1), and the ability to induce allogeneic T-lymphocyte proliferation in vitro, of a CD40- OSCC cell line, its CD40+ transfected derivative and null transfectants.


METHODS
OSCC cell lines and purified T lymphocytes were cocultured and T cell proliferation recorded. Phenotypes were analysed by flow cytometry.


RESULTS
After T lymphocyte proliferation, which all OSCC cell lines were able to induce, there was upregulation of MHC class II and ICAM-1. However, the CD40+ transfectants were the most immunologically potent and were the only cells to show increased expression of CD86 (as well as further upregulation of CD40 and a statistically insignificant rise in CD80). The effects of blocking antibodies on T-cell proliferation were only statistically significant with the CD40+ transfectants.


CONCLUSIONS
While not essential, expression of CD40 by OSCC cells is necessary for optimal induction of allogeneic T lymphocytes, possibly because of concurrent upregulation of other membrane-bound costimulatory molecules.
Villarroel Dorrego
M
M
Department of Oral Pathology, School of Clinical Dentistry, University of Sheffield, Sheffield S10 2TA, UK.
Whawell
S A
SA
Speight
P M
PM
Barrett
A W
AW
D015703:Antigens, CD / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D019013:CD40 Antigens / Q000235:genetics / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D049109:Cell Proliferation; D018920:Coculture Techniques; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D015603:Keratinocytes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D009062:Mouth Neoplasms / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000276:immunology
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D019013:CD40 Antigens; D000949:Histocompatibility Antigens Class II