title: [Studies on the seed embryo germination and propagation of Dendrobium candidum in vitro].
abstract: OBJECTIVE
  To determine optimum culture conditions for the seed embryo culture and rapid propagation of Dendrobium candidum.
  
  
  METHODS
  Seed embryos of D. candidum were incubated in the medium containing a combination of 6-benzylaminopurine (BA) and 1-naphthaleneacetic acid (NAA), potato extract, banana extract and activated carbon in order to induce seed embryo germination, protocorm differentiation, plantlet propagation and plantlet rooting.
  
  
  CONCLUSIONS
  The maximum embryo germination percentage was obtained in the 1/2 MS media supplemented with 20% potato extract. The 1/2 MS medium supplemented with 1.0 mg x L(-1) BA and 0.1 mg x L(-1) NAA was very beneficial to the protocorm differentiation and propagation of D. candidum. The highest protocorm propagation index was obtained from the medium containing the activated carbon. The highest root numbers and length were observed in plants growing in 1/2 MS medium containing 0.5 mg x L(-1) NAA.
authors.affiliation: Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China. tanggx@zju.edu.cn
authors.forename: Gui-xiang
authors.initials: GX
authors.lastname: Tang
authors.forename: Fu-deng
authors.initials: FD
authors.lastname: Wang
authors.forename: Wei-jun
authors.initials: WJ
authors.lastname: Zhou
mesh_terms: D001593:Benzyl Compounds; D002244:Carbon / Q000494:pharmacology; D003470:Culture Media; D031667:Dendrobium / Q000254:growth & development*; D018525:Germination / Q000187:drug effects*; D007701:Kinetin / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000254:growth & development*; D011687:Purines; D012639:Seeds / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D001593:Benzyl Compounds; D003470:Culture Media; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D002244:Carbon; C480551:benzylaminopurine; D007701:Kinetin