Effect of the urease accessory genes on activation of the Helicobacter pylori urease apoprotein.
The roles that accessory gene products play in activating the Helicobacter pylori urease apoprotein were examined. The activity of the urease apoprotein increased in the following order when it was expressed with the accessory genes: ureG<ureGH<ureFGH<ureEFGH<ureIEFGH. Moreover, stepwise additions of ureE and ureI to ureFGH significantly increased urease activity. Urease apoproteins coexpressed with ureFGH, ureEFGH, and ureIEFGH had similar low chymotrypsin susceptibilities. In vivo and in vitro activation studies showed that the cooperative effect of the accessory proteins involved processes in which the UreFGH complex, UreE, and UreI were implicated. Thus, the UreFGH complex may serve to alter the conformation of the apoprotein into one that is more competent to assemble a stable metallocenter, and that facilitates cooperative effects.
Park
Jeong-Uck
JU
Department of Microbiology, Gyeongsang National University College of Medicine, Jinju 660-701, Korea.
Song
Jae-Young
JY
Kwon
Young-Cheol
YC
Chung
Mi-Ja
MJ
Jun
Jin-Su
JS
Park
Jeong-Won
JW
Park
Seung-Gyu
SG
Hwang
Hyang-Ran
HR
Choi
Sang-Haeng
SH
Baik
Seung-Chul
SC
Kang
Hyung-Lyun
HL
Youn
Hee-Shang
HS
Lee
Won-Kon
WK
Cho
Myung-Je
MJ
Rhee
Kwang-Ho
KH
D001059:Apoproteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004789:Enzyme Activation; D015964:Gene Expression Regulation, Bacterial; D016480:Helicobacter pylori / Q000201:enzymology*; D015151:Immunoblotting; D028044:Phosphate-Binding Proteins; D010957:Plasmids; D014510:Urease / Q000235:genetics / Q000378:metabolism*
D001059:Apoproteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D028044:Phosphate-Binding Proteins; C086767:UreD protein, Bacteria; C090404:UreE protein, Bacteria; C082270:ureG protein, Bacteria; D014510:Urease