title: Bone formation-promoting effect of genistein on marrow mesenchymal cell culture.
abstract: The effect of genistein, a soybean isoflavone, on new bone formation by bone marrow cells from mature rats and humans was examined. Bone marrow cells were collected from the femoral diaphysis of 7-week-old Fisher rats, cultured in MEM containing fetal calf serum and then cultured with or without the addition of dexamethasone to the bone-forming medium. Genistein was added at concentrations of 10(-5),10(-6),10(-7) or 10(-8) M. Bone formation was examined 2 weeks after culture. After informed consent was obtained from a 55-year-old woman with lumbar spondylosis deformans, bone marrow cells were collected from her ilium for culture by the same process, and bone formation investigated. In both rats and humans, when dexamethasone was added to the bone-forming medium, genistein (10(-7) M and 10(-8) M) caused a significant increase in the levels of calcium, alkaline phosphatase, and DNA compared with cells not cultured in genistein. In conclusion, genistein was found to promote bone formation at lower concentrations across species, and thus may be useful as a bone formation-promoting factor.
authors.affiliation: Department of Diagnostic Pathology, Nara Medical University, Kashihara City, Nara 634-8522, Japan. o.noriko@tenriyorozu-hp.or.jp
authors.forename: Noriko
authors.initials: N
authors.lastname: Okumura
authors.forename: Takafumi
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Jin
authors.initials: J
authors.lastname: Iida
authors.forename: Akitaka
authors.initials: A
authors.lastname: Nonomura
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Takakura
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D019833:Genistein / Q000008:administration & dosage*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D019833:Genistein