Reaction on D-glucal by an inverting phosphorylase to synthesize derivatives of 2-deoxy-beta-D-arabino-hexopyranosyl-(1-->4)-D-glucose (2II-deoxycellobiose).
Four derivatives of 2(II)-deoxycellobiose were synthesized from d-glucal and acceptor sugars (d-glucose, d-xylose, d-mannose, and 2-deoxy-d-arabino-hexose) using a cellobiose phosphorylase from Cellvibrio gilvus. The enzyme was found to be an effective catalyst to synthesize the beta-(1-->4) linkage of 2-deoxy-d-arabino-hexopyranoside. The acceptor specificity for the d-glucal reaction was identical to that for the alpha-d-glucose 1-phosphate reaction, but the activity of d-glucal was approximately 500 times less than that of alpha-d-glucose 1-phosphate, using 10mM substrates.
Kitaoka
Motomitsu
M
Enzyme Laboratory, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. mkitaoka@nfri.affrc.go.jp
Nomura
Satoru
S
Yoshida
Michiteru
M
Hayashi
Kiyoshi
K
D002125:Calcium Gluconate / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002475:Cellobiose / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry*; D020633:Cellvibrio / Q000201:enzymology; D005964:Glucosyltransferases / Q000737:chemistry*; D006027:Glycosides / Q000138:chemical synthesis* / Q000737:chemistry*
C510409:2-deoxy-arabino-hexopyranosyl-1-4-glucose; D006027:Glycosides; D002475:Cellobiose; D005964:Glucosyltransferases; C034714:cellobiose phosphorylase; D002125:Calcium Gluconate