title: Parvovirus antibodies in vaccinated gilts in field conditions--results with HI and ELISA tests.
abstract: This study was conducted to determine the antibody response for porcine parvovirus (PPV) of 39 gilts in field conditions after vaccination. Gilts from four herds endemically infected with PPV were injected twice with a commercial vaccine of inactivated PPV and Erysipelothrix rhusiopathiae. The PPV antibodies were analysed both with haemagglutination inhibition (HI) and enzyme-linked immunosorbent assay (ELISA) in order to study the agreement between these methods. The possible association between high-antibody titres and reproductive failure (repeat breeding, culling for infertility, < or = 6 piglets born alive) was also investigated. In these study herds, endemically infected by PPV, most gilts (84.6%) had not seroconverted by the age of 6 months. On-field vaccination resulted in a consistent increase of humoral immunity not exceeding the antibody level of 1 : 512 in the majority of gilts in all herds examined. The agreement between ELISA and HI tests was moderate (Spearman's rho = 0.87, kappa = 0.63). The seroconversion over the level >1:512 by mid-pregnancy was not associated with reproductive failure.
authors.affiliation: Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Saari Unit, Helsinki, Finland. jonna.oravainen@helsinki.fi
authors.forename: J
authors.initials: J
authors.lastname: Oravainen
authors.forename: M
authors.initials: M
authors.lastname: Hakala
authors.forename: E
authors.initials: E
authors.lastname: Rautiainen
authors.forename: P
authors.initials: P
authors.lastname: Veijalainen
authors.forename: M
authors.initials: M
authors.lastname: Heinonen
authors.forename: A
authors.initials: A
authors.lastname: Tast
authors.forename: J V
authors.initials: JV
authors.lastname: Virolainen
authors.forename: O A T
authors.initials: OA
authors.lastname: Peltoniemi
mesh_terms: D000372:Agglutination Tests / Q000379:methods / Q000592:standards / Q000662:veterinary*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D005260:Female; D010322:Parvoviridae Infections / Q000517:prevention & control / Q000662:veterinary; D028323:Parvovirus, Porcine / Q000276:immunology*; D015203:Reproducibility of Results; D012098:Reproduction / Q000276:immunology / Q000502:physiology*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines