title: In vivo magnetic resonance volumetric and spectroscopic analysis of mouse prostate cancer models.
abstract: BACKGROUND
  Mouse prostate cancer modeling presents unique obstacles to the study of spontaneous tumor initiation and progression due to the anatomical location of the tissue.
  
  
  RESULTS
  High resolution (130 microm(x) x 130 microm(y) x 300 microm(z)), three-dimensional MRI allowed for the visualization, segmentation, and volumetric measurement of the prostate from normal and genetically engineered animals, in vivo. Additionally, MRS performed on the prostate epithelia of probasin-ErbB-2Delta x Pten(+/-) mice identified changes in the relative concentrations of the metabolites choline and citrate, which was not observed in TRAMP mice.
  
  
  METHODS
  T1-weighted MRI was performed on normal, TRAMP, probasin-ErbB-2/Her2/Neu (probasin-ErbB-2Delta), and probasin-ErbB-2Delta in the context of decreased Pten activity (probasin-ErbB-2Delta x Pten(+/-)) mice. Volume-localized single-voxel proton magnetic resonance spectroscopy (SVS (1)H MRS) was also performed.
  
  
  CONCLUSIONS
  The data presented supports the use of combined MRI and MRS for the measurement of biochemical and morphometric alterations in mouse models of prostate cancer.
authors.affiliation: Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA.
authors.forename: Stanley T
authors.initials: ST
authors.lastname: Fricke
authors.forename: Olga
authors.initials: O
authors.lastname: Rodriguez
authors.forename: John
authors.initials: J
authors.lastname: Vanmeter
authors.forename: Luis E
authors.initials: LE
authors.lastname: Dettin
authors.forename: Mathew
authors.initials: M
authors.lastname: Casimiro
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Chien
authors.forename: Tionanatasha
authors.initials: T
authors.lastname: Newell
authors.forename: Kevin
authors.initials: K
authors.lastname: Johnson
authors.forename: Lilia
authors.initials: L
authors.lastname: Ileva
authors.forename: John
authors.initials: J
authors.lastname: Ojeifo
authors.forename: Michael D
authors.initials: MD
authors.lastname: Johnson
authors.forename: Chris
authors.initials: C
authors.lastname: Albanese
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D021621:Imaging, Three-Dimensional; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000662:veterinary*