Crystal structure of PilF: functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa.
PilF is a requisite protein involved in the type 4 pilus biogenesis system from the Gram-negative human pathogenic bacteria, Pseudomonas aeruginosa. We determined the PilF structure at a 2.2A resolution; this includes six tandem tetratrico peptide repeat (TPR) units forming right-handed superhelix. PilF structure was similar to the heat shock protein organizing protein, which interacts with the C-terminal peptide of Hsp90 and Hsp70 via a concave Asn ladder in the inner groove of TPR superhelix. After simulated screening, the C-terminal pentapeptides of PilG, PilU, PilY, and PilZ proved to be a likely candidate binding to PilF, which are ones of 25 necessary components involved in the type 4 pilus biogenesis system. We proposed that PilF would be critical as a bridgehead in protein-protein interaction and thereby, PilF may bind a necessary molecule in type 4 pilus biogenesis system such as PilG, PilU, PilY, and PilZ.
Kim
Kyunggon
K
Division of Molecular Genomic Medicine, College of Medicine, Seoul National University, Yongon-Dong, Seoul 110-799, Republic of Korea.
Oh
Jongkil
J
Han
Dohyun
D
Kim
Eunice EunKyeong
EE
Lee
Byungil
B
Kim
Youngsoo
Y
D000595:Amino Acid Sequence; D001665:Binding Sites; D003198:Computer Simulation; D003461:Crystallography; D019281:Dimerization; D036781:Fimbriae Proteins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*; D010861:Fimbriae, Bacterial / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000737:chemistry / Q000648:ultrastructure; D011485:Protein Binding; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure; D013329:Structure-Activity Relationship
D046912:Multiprotein Complexes; C102919:PilF protein, Pseudomonas; D036781:Fimbriae Proteins