Conversion of 2-(4-carboxyphenyl)-6-nitrobenzothiazole to 4-(6-amino-5-hydroxybenzothiazol-2-yl)benzoic acid by a recombinant E. coli strain.
E. coli C43(DE3)pNbzAHabA, expressing nitroreductase and mutase enzymes, converts 2-(4-carboxyphenyl)-6-nitrobenzothiazole to 4-(6-amino-5-hydroxybenzothiazol-2-yl)benzoic acid.
Nadeau
Lloyd J
LJ
AFRL/MLQL, Tyndall AFB, 139 Barnes Dr., Building 1117, FL 32403, USA. lloyd.nadeau@tyndall.af.mil
Spain
Jim C
JC
Kannan
Ramamurthi
R
Tan
Loon-Seng
LS
D001565:Benzoates / Q000138:chemical synthesis* / Q000378:metabolism; D052160:Benzothiazoles / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D019751:Intramolecular Transferases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009601:Nitroreductases / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
C519052:2-(4-carboxyphenyl)-6-nitrobenzothiazole; C519051:4-(6-amino-5-hydroxybenzothiazol-2-yl)benzoic acid; D001565:Benzoates; D052160:Benzothiazoles; D011994:Recombinant Proteins; D009601:Nitroreductases; D019751:Intramolecular Transferases