title: Oral vaccination against Helicobacter pylori infection is not effective in mice with Fas ligand deficiency.
abstract: The aim of this study was to delineate the role of the Fas pathway in vaccination against Helicobacter pylori. C57BL/6 and Fas ligand-deficient (gld) mice were divided into 3 groups: control, H. pylori infected, and orally vaccinated (H. pylori whole cell sonicate and cholera toxin adjuvant). Oral vaccination prevented H. pylori colonization in 78% of C57BL/6 mice compared to only 18% of gld mice. Vaccination did not alter the degree of apoptosis in either strain of mice. Vaccination led to significant increase in interleukin (IL)-5 and IL-10 in C57BL/6 but not gld mice. H. pylori infection increased interferon (IFN)-gamma levels in C57BL/6 but not in gld mice while vaccination had no effect on IFN-gamma levels in either strain. Oral vaccination is not effective in Fas ligand-deficient mice likely owing to lack of effective cytokine responses. This indicates that the Fas pathway plays a critical role in promoting an appropriate effector response following H. pylori vaccination.
authors.affiliation: Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Yaron
authors.initials: Y
authors.lastname: Avitzur
authors.forename: Esther
authors.initials: E
authors.lastname: Galindo-Mata
authors.forename: Nicola L
authors.initials: NL
authors.lastname: Jones
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology; D001428:Bacterial Vaccines / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D053222:Fas Ligand Protein; D016481:Helicobacter Infections / Q000276:immunology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000276:immunology*; D007378:Interleukins / Q000032:analysis / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000172:deficiency* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D012016:Reference Values; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D017211:Treatment Failure; D048069:Tumor Necrosis Factors / Q000172:deficiency* / Q000276:immunology; D014611:Vaccination / Q000379:methods
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D007378:Interleukins; D008562:Membrane Glycoproteins; D048069:Tumor Necrosis Factors