Use of microsatellite markers to detect quantitative trait loci in Yorkshire pigs.
To identify genetic markers associated with economic traits in pigs, 157 microsatellite markers were examined in Yorkshire pigs. Thirty eight female Yorkshire pigs were initially examined and six of them were selected as progenitors; half were more than 1.5 standard deviations (SD) above the mean for average daily gain (ADG) and backfat thickness (BFT), and the remaining half were more than 1.5 SD below the mean. These pigs were then mated to male Duroc pigs, and 200 F2 pig offspring were examined for the association of specific alleles with ADG and BFT. To confirm the specific markers identified in the initial analysis, associations of significant markers with economic traits were further examined in 228 additional performance-tested purebred pigs. Twenty-five microsatellite markers were significantly associated with either ADG or BFT, and among these, 17 were associated with both traits. The markers with the highest association to ADG were also associated with BFT. Our study reveals that specific markers could be used to predict economic significance, and confirms several quantitative trait loci (QTL) identified in previous studies. However, further analysis with more closely-spaced microsatellite markers is required to refine predictive values for economic traits and positions of QTL that are reliable for actual phenotypic prediction.
Kim
Chul Wook
CW
Department of Animal Resources Technology, Jinju National University, Gyeongnam, Korea. cwkim@jinju.ac.kr
Hong
Yeon Hee
YH
Yun
Sung-Il
SI
Lee
Sang-Rae
SR
Kim
Young Hyun
YH
Kim
Myeong-Su
MS
Chung
Ki Hwa
KH
Jung
Won Youg
WY
Kwon
Eun Jung
EJ
Hwang
Sun Sook
SS
Park
Da Hye
DH
Cho
Kwang Keun
KK
Lee
Jung Gyu
JG
Kim
Byeong Woo
BW
Kim
Jae Woo
JW
Kang
Yang Su
YS
Yeo
Jung Sou
JS
Chang
Kyu-Tae
KT
D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D005260:Female; D005819:Genetic Markers; D005821:Genetic Techniques; D016678:Genome; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats; D008957:Models, Genetic; D015233:Models, Statistical; D010641:Phenotype; D016133:Polymerase Chain Reaction; D040641:Quantitative Trait Loci; D013552:Swine
D005819:Genetic Markers