title: Quartz crystal microbalance bioaffinity sensor for rapid identification of glycosyldisulfide lectin inhibitors from a dynamic combinatorial library.
abstract: Carbohydrate-lectin interactions were probed with dynamic combinatorial libraries, using the plant lectin Concanavalin A as target species. The dynamic combinatorial libraries were generated from a pool of thiol components through reversible thiol-disulfide interchange, and screened using a simple and efficient method based on a quartz crystal microbalance setup. It was found that dimers based on 1-thio- and 6-thio-mannose analogues were the most active inhibitors. Furthermore, the results clearly show that the 6-thio-mannose possess unique characteristics compared to its oxygen-containing counterpart.
authors.affiliation: KTH-Royal Institute of Technology, Department of Chemistry, Teknikringen 30, S-10044 Stockholm, Sweden.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Pei
authors.forename: Rikard
authors.initials: R
authors.lastname: Larsson
authors.forename: Teodor
authors.initials: T
authors.lastname: Aastrup
authors.forename: Henrik
authors.initials: H
authors.lastname: Anderson
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Lehn
authors.forename: Olof
authors.initials: O
authors.lastname: Ramström
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D020650:Combinatorial Chemistry Techniques; D037102:Lectins / Q000037:antagonists & inhibitors*
chemical_list: D037102:Lectins