Gene expression profiling of early follicular development in primordial, primary, and secondary follicles.
OBJECTIVE
To study global gene expression profiles of early folliculogenesis in primordial, primary, and secondary follicles.


METHODS
A cDNA microarray study using amplified RNAs from isolated follicles.


METHODS
Experimental animal study.


METHODS
Female ICR strain mice (12 days old).


METHODS
Isolation of follicles at each stage, RNA isolation and amplification, microarray hybridization, and statistical analysis for microarray.


METHODS
Gene lists of various functional groups with an estimated false discovery rate of 5%. Among them, platelet-derived growth factors (PDGFs) and receptors were localized by immunohistochemistry in mouse ovaries.


RESULTS
We analyzed a list of genes according to function, such as apoptosis, cell cycle, cell proliferation and maintenance, cytoskeleton, extracellular matrix, and signal transduction, as well as according to frequency. Among the list of genes, we found all PDGFs (A, B, C, and D) and receptors (alpha and beta) are expressed with differential expression patterns in the oocytes and ovarian cells according to stage of follicular development.


CONCLUSIONS
The present report suggests that genome-wide expression profiling using microarray after RNA amplification may become a useful tool to better understand the molecular mechanism(s) involved in early ovarian folliculogenesis.
Yoon
Se-Jin
SJ
CHA Research Institute, Infertility Medical Center, CHA General Hospital, Seoul, Korea.
Kim
Kyeoung-Hwa
KH
Chung
Hyung-Min
HM
Choi
Dong-Hee
DH
Lee
Woo-Sik
WS
Cha
Kwang-Yul
KY
Lee
Kyung-Ah
KA
D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D051379:Mice; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development* / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics
D010982:Platelet-Derived Growth Factor; D017479:Receptors, Platelet-Derived Growth Factor