Assay and functional analysis of dynamin-like Mx proteins.
Mx proteins are interferon-induced large guanosine triphosphatases (GTPases) that share structural and functional properties with dynamin and dynamin-like proteins, such as self-assembly and association with intracellular membranes. A unique property of some Mx proteins is their antiviral activity against a range of RNA viruses, including influenza viruses and members of the bunyavirus family. These viruses are inhibited at an early stage in their life cycle, soon after host cell entry and before genome amplification. The association of the human MxA GTPase with membranes of the endoplasmic reticulum seems to support its antiviral function by providing an interaction platform that facilitates viral target recognition, MxA oligomerization, and missorting of the resulting multiprotein complex into large intracellular aggregates.
Kochs
Georg
G
Department of Virology, University of Freiburg, Germany.
Reichelt
Mike
M
Danino
Dganit
D
Hinshaw
Jenny E
JE
Haller
Otto
O
D016475:3T3 Cells; D000818:Animals; D002522:Chlorocebus aethiops; D020285:Cryoelectron Microscopy; D034281:Dynamins / Q000032:analysis* / Q000502:physiology; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000502:physiology; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000502:physiology; D051379:Mice; D046529:Microscopy, Electron, Transmission; D064547:Myxovirus Resistance Proteins; D020836:Protein Structure, Quaternary; D014709:Vero Cells
C579071:MX1 protein, human; D008565:Membrane Proteins; C579070:Mx1 protein, mouse; D064547:Myxovirus Resistance Proteins; D019204:GTP-Binding Proteins; D034281:Dynamins