title: Non-structural proteins of dengue 2 virus offer limited protection to interferon-deficient mice after dengue 2 virus challenge.
abstract: Chimeric (D2/WN) viruses containing the pre-membrane (prM) and envelope (E) proteins of West Nile virus (WN virus) and the capsid (C) and non-structural proteins of dengue 2 (DEN2) virus were used to evaluate the protective immunity elicited by either the flaviviral E protein or non-structural proteins. AG129 interferon-deficient mice, previously shown to be protected against lethal DEN1 or DEN2 viral infection after vaccination with a wild-type or candidate vaccine strain of DEN1 or DEN2 virus, respectively, were immunized with chimeric D2/WN virus and then challenged with DEN2 virus. D2/WN chimeric viruses were non-pathogenic in AG129 mice. These viruses elicited little anti-DEN E antibody, high levels of anti-DEN NS1 antibody and no or very low levels of DEN2 virus-neutralizing antibodies. Only 15% of D2/WN-immunized mice survived challenge with DEN2 virus. However, their mean survival time increased by 11-14 days over non-immunized controls. These results suggest that, whilst the non-structural proteins were able to enhance mean survival times of AG129 mice, this protection was not as effective as protection mediated by the E protein.
authors.affiliation: Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention (CDC), Public Health Service, US Department of Health and Human Services, PO Box 2087, Fort Collins, CO 80522, USA.
authors.forename: Amanda E
authors.initials: AE
authors.lastname: Calvert
authors.affiliation: Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention (CDC), Public Health Service, US Department of Health and Human Services, PO Box 2087, Fort Collins, CO 80522, USA.
authors.forename: Claire Y-H
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention (CDC), Public Health Service, US Department of Health and Human Services, PO Box 2087, Fort Collins, CO 80522, USA.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Kinney
authors.affiliation: Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention (CDC), Public Health Service, US Department of Health and Human Services, PO Box 2087, Fort Collins, CO 80522, USA.
authors.forename: John T
authors.initials: JT
authors.lastname: Roehrig
mesh_terms: D000818:Animals; D002678:Chimera; D002522:Chlorocebus aethiops; D003715:Dengue / Q000517:prevention & control*; D003716:Dengue Virus / Q000737:chemistry*; D016679:Genome, Viral; D007372:Interferons / Q000172:deficiency* / Q000378:metabolism; D051379:Mice; D014614:Vaccines, Synthetic / Q000276:immunology*; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000008:administration & dosage* / Q000235:genetics; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D014614:Vaccines, Synthetic; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines; D007372:Interferons