Imaging platforms for measurement of membrane trafficking.
In this chapter we describe automated imaging methods used to measure the transport of an established membrane transport marker from the endoplasmic reticulum to the plasma membrane. The method is fast and significantly robust to be applied in systematic studies on a large scale such as genome-wide screening projects. We further describe the use of software macros and plugins in Image J that allow the quantification of the kinetics of membrane transport intermediates in fluorescence microscopy time-lapse sequences.
Pepperkok
Rainer
R
Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany.
Simpson
Jeremy C
JC
Rietdorf
Jens
J
Cetin
Cihan
C
Liebel
Urban
U
Terjung
Stefan
S
Zimmermann
Timo
T
D000256:Adenoviridae / Q000235:genetics; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods*; D021381:Protein Transport / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics; D014764:Viral Proteins / Q000378:metabolism
D006023:Glycoproteins; D034741:RNA, Small Interfering; D014764:Viral Proteins