High-throughput assay for small molecules that modulate zebrafish embryonic heart rate.
To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.
Burns
C Geoffrey
CG
Developmental Biology Laboratory, Cardiovascular Research Center, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts 02129, USA. gburns@cvrc.mgh.harvard.edu
Milan
David J
DJ
Grande
Eric J
EJ
Rottbauer
Wolfgang
W
MacRae
Calum A
CA
Fishman
Mark C
MC
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002317:Cardiovascular Agents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004625:Embryo, Nonmammalian / Q000502:physiology; D005240:Feasibility Studies; D049452:Green Fluorescent Proteins / Q000032:analysis*; D006321:Heart / Q000187:drug effects* / Q000196:embryology / Q000502:physiology*; D006339:Heart Rate / Q000187:drug effects* / Q000502:physiology; D009206:Myocardium / Q000378:metabolism; D013997:Time Factors; D015027:Zebrafish
D002317:Cardiovascular Agents; D049452:Green Fluorescent Proteins