Protein microarrays: a new tool for profiling antibody cross-reactivity.
Antibody cross-reactivity can compromise interpretation of experiments and derail therapeutic antibody development. Standard techniques such as immunohistochemistry or Western analysis provide important but often inadequate approaches to assess antibody specificity. Protein microarrays are providing a new approach to rapidly characterize antibody cross-reactivity against 1,000s of proteins simultaneously. This review will focus on reported examples of antibody cross-reactivity, methods used to characterize them, and the recent development and use of protein microarrays for assessing antibody specificity.
Predki
Paul F
PF
Invitrogen Protein Microarray Center, 688 E Main St, Branford, CT 06405, USA. paul.predki@invitrogen.com
Mattoon
Dawn
D
Bangham
Rhonda
R
Schweitzer
Barry
B
Michaud
Gregory
G
D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D040081:Protein Array Analysis