title: Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX prophage.
abstract: Vibrio cholerae O1 isolates belonging to the Ogawa serotype, El Tor biotype, harbouring the classical CTX prophage were first isolated in Mozambique in 2004. Multilocus sequence typing (MLST) analysis using nine genetic loci showed that the Mozambique isolates have the same sequence type (ST) as O1 El Tor N16961, a representative of the current seventh cholera pandemic. Analysis of the CTX prophage in the Mozambique isolates indicated that there is one type of rstR in these isolates: the classical CTX prophage. It was also found that the ctxB-rstR-rstA-rstB-phs-cep fragment was PCR-amplified from these isolates, which indicates the presence of a tandem repeat of the classical CTX prophage in the genome of the Mozambique isolates. The possible origin of these isolates and the presence of the tandem repeat of the classical prophage in them implicate the presence of the classical CTX phage.
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, 151-742, Republic of Korea.|International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
authors.forename: Je Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Han
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, 151-742, Republic of Korea.|International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
authors.forename: Seon Young
authors.initials: SY
authors.lastname: Choi
authors.affiliation: Ministry of Health, Maputo, Mozambique.
authors.forename: Marcelino E S
authors.initials: MES
authors.lastname: Lucas
authors.affiliation: Centro De Higiene Ambiental E Exames Medicos, Rua Correia De Brito N° 1815 Sofala - Beira, Mozambique.
authors.forename: C
authors.initials: C
authors.lastname: Mondlane
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
authors.forename: M
authors.initials: M
authors.lastname: Ansaruzzaman
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
authors.forename: G Balakrish
authors.initials: GB
authors.lastname: Nair
authors.affiliation: International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
authors.forename: David A
authors.initials: DA
authors.lastname: Sack
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
authors.forename: Lorenz
authors.initials: L
authors.lastname: von Seidlein
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
authors.forename: John D
authors.initials: JD
authors.lastname: Clemens
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
authors.forename: Manki
authors.initials: M
authors.lastname: Song
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, 151-742, Republic of Korea.|International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
authors.forename: Jongsik
authors.initials: J
authors.lastname: Chun
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
authors.lastname: The Mozambique Cholera Vaccine Demonstration Project Coordination Group
authors.affiliation: International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul, 151-818, Republic of Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics; D014644:Genetic Variation; D016680:Genome, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009073:Mozambique; D039002:Prophages / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020080:Tandem Repeat Sequences / Q000235:genetics; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; C107871:RstR protein, Vibrio cholerae