title: Identification and quantitative determination of morphine in the urine of heroin dependents.
abstract: Morphine in the urine of heroin addicts was identified by TLC using methanol, water, benzene and glacial acetic acid (80:15:15:5) and ethyl acetate, methanol and ammonium hydroxide (25:10:5) as solvent systems and potassium iodoplatinate and iodine as the developing reagents. Potassium iodate in alkaline medium gives a yellowish golden colour having maximum absorbance at 450 nm. By this reaction the minimum limit of identification and quantitative determination of morphine is 2 microg/ml. It provides a basis for the spectrophotometric determination of morphine in the urine of heroin addicts.
authors.affiliation: Faculty of Pharmacy, University of the Punjab, Lahore, Pakistan.
authors.forename: A
authors.initials: A
authors.lastname: Rasheed
authors.forename: S A
authors.initials: SA
authors.lastname: Lali
authors.forename: H R
authors.initials: HR
authors.lastname: Chaudhry