Liquid chromatography/mass spectrometry and liquid chromatography/nuclear magnetic resonance as complementary analytical techniques for unambiguous identification of polymethoxylated flavones in residues from molecular distillation of orange peel oils (Citrus sinensis).
Liquid chromatography/mass spectrometry and liquid chromatography/nuclear magnetic resonance techniques with ultraviolet/diode array detection were used as complementary analytical tools for the reliable identification of polymethoxylated flavones in residues from molecular distillation of cold-pressed peel oils of Citrus sinensis. After development of a liquid chromatographic separation procedure, the presence of several polymethoxy flavones such as sinensetin, nobiletin, tangeretin, quercetogetin, heptamethoxyflavone, and other derivatives was unambiguously confirmed. In addition, proceranone, an acetylated tetranortriterpenoid with limonoid structure, was identified for the first time in citrus.
Weber
Berthold
B
Symrise GmbH and Co. KG, Corporate Research Division, 37601 Holzminden, Germany. berthold.weber@symrise.com
Hartmann
Beate
B
Stöckigt
Detlef
D
Schreiber
Klaus
K
Roloff
Michael
M
Bertram
Heinz-Jürgen
HJ
Schmidt
Claus O
CO
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032084:Citrus sinensis / Q000737:chemistry*; D047309:Flavones / Q000032:analysis* / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010938:Plant Oils / Q000737:chemistry*
D047309:Flavones; D010938:Plant Oils