Immunohistochemical analysis of histone H3 acetylation and methylation--evidence for altered epigenetic signaling following traumatic brain injury in immature rats.
Posttranslational modifications (PTMs) of histone proteins may result in altered epigenetic signaling after pediatric traumatic brain injury (TBI). Hippocampal histone H3 acetylation and methylation in immature rats after moderate TBI were measured and decreased only in CA3 at 6 h and 24 h with persistent methylation decreases up to 72 h after injury. Decreased histone H3 acetylation and methylation suggest altered hippocampal CA3 epigenetic signaling during the first hours to days after TBI.
Gao
Wei-Min
WM
Neurological Surgery, U. Pittsburgh, Pittsburgh, PA 15260, USA.
Chadha
Mandeep S
MS
Kline
Anthony E
AE
Clark
Robert S B
RS
Kochanek
Patrick M
PM
Dixon
C Edward
CE
Jenkins
Larry W
LW
D000107:Acetylation; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001930:Brain Injuries / Q000235:genetics* / Q000378:metabolism*; D044127:Epigenesis, Genetic; D006624:Hippocampus / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008745:Methylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013997:Time Factors
D006657:Histones