Clathrin-coated vesicle formation from isolated plasma membranes.
Endocytic clathrin-coated vesicle (CCV) formation is a complex process involving a large number of proteins and lipids. The minimum machinery and the hierarchy of the events involved in CCV formation have yet to be defined. Here we describe an in vitro assay for CCV formation from highly purified rat liver plasma membranes. This rapid and easy assay can be used to quantitatively evaluate the different protein requirements for different endocytic receptors.
Miwako
Ishido
I
The Scripps Research Institute, Department of Cell Biology, La Jolla, California, USA.
Schmid
Sandra L
SL
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000648:ultrastructure*; D022163:Clathrin-Coated Vesicles / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology*; D026561:Low Density Lipoprotein Receptor-Related Protein-2 / Q000187:drug effects / Q000378:metabolism; D051381:Rats
D026561:Low Density Lipoprotein Receptor-Related Protein-2