Biological determinants of and reference values for plasma interleukin-8, monocyte chemoattractant protein-1, epidermal growth factor, and vascular endothelial growth factor: Results from the STANISLAS cohort.
BACKGROUND
Interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF) are known to be involved in various diseases related to inflammation, vascular remodeling, or growth deregulation. In addition, increases in plasma concentrations of these cytokines appear to provide useful diagnostic and prognostic information. We therefore investigated which factors most strongly influence the biological variations of plasma IL-8, MCP-1, EGF, and VEGF concentrations.


METHODS
We used the Evidence biochip array analyzer to quantify plasma IL-8, MCP-1, EGF, and VEGF concentrations in a subsample of 304 children (age range, 4-17 years) and 540 adults (age range, 18-55 years) from the STANISLAS family study. We also calculated reference intervals for the 4 cytokines.


RESULTS
We found the following associations with plasma marker concentrations: Age, neutrophil count, and glucose concentration were positively associated with IL-8 concentrations in children and adults, as were smoking and platelet count in adults. MCP-1 concentrations were associated with age and smoking in both children and adults, monocyte count in children, and sex and hematocrit in adults. EGF concentrations were associated with platelet count in children and monocyte count and glucose in adults. VEGF concentrations were associated with age in children and adults and platelet count and alanine aminotransferase activity in adults.


CONCLUSIONS
Our results for IL-8, MCP-1, EGF, and VEGF may be useful for interpretation of patients' laboratory results and for understanding the regulation of concentrations of these cytokines in physiologic conditions.
Berrahmoune
Hind
H
INSERM U525, Nancy, France.
Lamont
John V
JV
Herbeth
Bernard
B
FitzGerald
Peter S
PS
Visvikis-Siest
Sophie
S
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D018932:Chemokine CCL2 / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004815:Epidermal Growth Factor / Q000097:blood*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010949:Plasma; D012016:Reference Values; D012044:Regression Analysis; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
D015415:Biomarkers; D018932:Chemokine CCL2; D016209:Interleukin-8; D042461:Vascular Endothelial Growth Factor A; D004815:Epidermal Growth Factor