Characterization of Methylophaga sp. strain SK1 cytochrome cL expressed in Escherichia coli.
Methylophaga sp. strain SK1 is a new restricted facultative methanol-oxidizing bacterium that was isolated from seawater. The aim of this study was to characterize the electron carriers involved in the methanol oxidation process in Methylophaga sp. strain SK1. The gene encoding cytochrome c(L) (mxaG) was cloned and the recombinant gene was expressed in Escherichia coli DH5 under strict anaerobic conditions. The recombinant cytochrome c(L) had the same molecular weight and absorption spectra as the wild-type cytochrome c(L) both in the reduced and oxidized forms. The electron flow rate from methanol dehydrogenase (MDH) to the recombinant cytochrome c(L) was similar to that from MDH to the wild-type cytochrome c(L). These results suggest that recombinant cytochrome c(L) acts as a physiological primary electron acceptor for MDH.
Kim
Hee Gon
HG
Department of Biomaterials Engineering, Chosum University, Gwangju, Republic of Korea.
Phan
Trong Nhat
TN
Jang
Tae Sa
TS
Koh
Moonjoo
M
Kim
Si Wouk
SW
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000693:Anaerobiosis; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008970:Molecular Weight; D044147:Piscirickettsiaceae / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012623:Seawater / Q000382:microbiology; D013057:Spectrum Analysis; D014871:Water Microbiology
D003574:Cytochrome c Group; D011994:Recombinant Proteins; C058498:cytochrome C(L); D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)