Analysis on the molecular species and concentration of circulating ADAMTS13 in Blood.
ADAMTS13 is the metalloprotease responsible for the proteolytic degradation of von Willebrand factor (VWF). A severe deficiency of this VWF-cleaving protease activity causes thrombotic thrombocytopenic purpura. This protease, comprising 1,427 amino acid residues, is composed of multiple domains, i.e., a preproregion, a metalloprotease domain, a disintegrin-like domain, a thrombospondin type-1 motif (Tsp1), a cysteine-rich domain, a spacer domain, seven Tsp1 repeats, and two CUB domains. We prepared one polyclonal and seven monoclonal antibodies recognizing distinct epitopes spanning the entire ADAMTS13 molecule. Of these antibodies, two of the monoclonal ones, which recognize the disintegrin-like and cysteine-rich/spacer domains, respectively, abolished the hydrolytic activity of ADAMTS13 toward both a synthetic substrate, FRETS-VWF73, and the natural substrate, VWF. In addition, these antibodies blocked the binding of ADAMTS13 to VWF. These results revealed that the region between the disintegrin-like and cysteine-rich/spacer domains interacts with VWF. Employing these established polyclonal and monoclonal antibodies, we examined the molecular species of ADAMTS13 circulating in the blood by immunoprecipitation followed by Western blot analysis, and estimated the plasma concentration of ADAMTS13 by enzyme-linked immunosorbent assay. These studies indicated that the major fraction of ADAMTS13 in blood plasma consisted of the full-length form. The concentration of ADAMTS13 in normal plasma was approximately 0.5-1 microg/ml.
Soejima
Kenji
K
Research Department 1, The Chemo-Sero-Therapeutic Research Institute, Kyokushikawabe, Kikuchi, Kumamoto 869-1298. soejima@kaketsuken.or.jp
Nakamura
Hitomi
H
Hirashima
Masaki
M
Morikawa
Wataru
W
Nozaki
Chikateru
C
Nakagaki
Tomohiro
T
D051722:ADAM Proteins / Q000097:blood* / Q000276:immunology; D000071120:ADAMTS13 Protein; D015153:Blotting, Western; D017077:Culture Media, Conditioned; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D006801:Humans; D047468:Immunoprecipitation; D009500:Neutralization Tests
D017077:Culture Media, Conditioned; D000939:Epitopes; D051722:ADAM Proteins; D000071120:ADAMTS13 Protein; C099604:ADAMTS13 protein, human