BEAMing up for detection and quantification of rare sequence variants.
BEAMing allows the one-to-one conversion of a population of DNA fragments into a population of beads. We used rolling circle amplification to increase the number of copies bound to such beads by more than 100-fold. This allowed enumeration of mutant and wild-type sequences even when they were present at ratios less than 1:10,000 and was sensitive enough to directly quantify the error rate of DNA polymerases used for PCR.
Li
Meng
M
The Sidney Kimmel Comprehensive Cancer Center, Howard Hughes Medical Institute, and Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 1650 Orleans Street, Baltimore, Maryland 21231, USA.
Diehl
Frank
F
Dressman
Devin
D
Vogelstein
Bert
B
Kinzler
Kenneth W
KW
D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004252:DNA Mutational Analysis / Q000379:methods*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004655:Emulsions / Q000737:chemistry; D005091:Exons / Q000235:genetics; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008863:Microspheres; D009154:Mutation / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009821:Oils / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014867:Water / Q000737:chemistry
D004655:Emulsions; D005456:Fluorescent Dyes; D009821:Oils; D016159:Tumor Suppressor Protein p53; D014867:Water; D004247:DNA; D004259:DNA-Directed DNA Polymerase