title: Deoxyribozyme-based three-input logic gates and construction of a molecular full adder.
abstract: We have developed an array of seven deoxyribozyme-based molecular logic gates that behaves as a full adder in a single solution, with three oligonucleotides as inputs and two independent fluorogenic cleavage reactions as carry and sum outputs. The sum output consisted of four new deoxyribozyme-based logic gates: an ANDAND gate and three ANDNOTANDNOT gates. These gates required the design of a generic three-input deoxyribozyme-based logic gate that can use any three-way combination of activating or inactivating inputs. This generic gate design utilizes an additional inverting element that hybridizes to convert YES logic into NOT logic and vice versa. The system represents the first solution-phase, single test tube, enzymatic full adder and shows the complexity of control over molecular scale events that can be achieved with deoxyribozyme-based logic gates. Similar systems could be applied to control autonomous therapeutic and diagnostic devices.
authors.affiliation: Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, Box 84, 630 West 168th Street, New York, New York 10032, USA.
authors.forename: Harvey
authors.initials: H
authors.lastname: Lederman
authors.forename: Joanne
authors.initials: J
authors.lastname: Macdonald
authors.forename: Darko
authors.initials: D
authors.lastname: Stefanovic
authors.forename: Milan N
authors.initials: MN
authors.lastname: Stojanovic
mesh_terms: D001483:Base Sequence; D019295:Computational Biology; D021881:DNA, Catalytic / Q000737:chemistry*; D008128:Logic; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D013997:Time Factors
chemical_list: D021881:DNA, Catalytic; D009841:Oligonucleotides; D012996:Solutions