title: Aldosterone increases voltage-gated sodium current in ventricular myocytes.
abstract: The role of aldosterone in the pathogenesis of heart failure (HF) is still poorly understood. Recently, aldosterone has been shown to modulate the function of cardiac Ca(2+) and K(+) channels, thus playing a role in the electrical remodeling process. The goal of this work was to investigate the role of aldosterone on the cardiac Na(+) current (I(Na)). We analyzed the effects of aldosterone on I(Na) in isolated adult mouse ventricular myocytes, using the whole cell patch-clamp technique. After 24 h incubation with 1 microM aldosterone, the I(Na) density was significantly increased (+55%), without alteration of the biophysical properties and the cell membrane capacitance. Aldosterone (10 nM) increased the I(Na) by 23%. In 24-h coincubation experiments, with the use of actinomycin D, cycloheximide, or brefeldin A, the effect of aldosterone on I(Na) was abolished. Spironolactone (mineralocorticoid receptor antagonist, 10 microM) prevented the 1 microM aldosterone-dependent I(Na) increase, whereas RU-38486 (glucocorticoid receptor antagonist, 10 microM) did not. The action potential duration (APD) was longer in aldosterone-treated (APD(90): +53%) than in control myocytes. In addition, the L-type Ca(2+) current was also upregulated (+48%). We performed quantitative RT-PCR measurements and Western blots to quantify the mRNA and protein levels of Na(v)1.5 and Ca(v)1.2 (main channels mediating cardiac I(Na) and I(Ca)), but no significant difference was found. In conclusion, this study shows that aldosterone upregulates the cardiac I(Na) and suggest that this phenomenon may contribute to the HF-induced electrical remodeling process that may be reversed by spironolactone.
authors.affiliation: Department of Pharmacology and Toxicology, University of Lausanne, Bugnon 27, 1005 Lausanne, Switzerland.
authors.forename: Christophe
authors.initials: C
authors.lastname: Boixel
authors.forename: Bruno
authors.initials: B
authors.lastname: Gavillet
authors.forename: Jean-Sébastien
authors.initials: JS
authors.lastname: Rougier
authors.forename: Hugues
authors.initials: H
authors.lastname: Abriel
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D002120:Calcium Channel Agonists / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects; D015640:Ion Channel Gating / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D062687:Sodium Channel Agonists; D015222:Sodium Channels / Q000096:biosynthesis
chemical_list: D002120:Calcium Channel Agonists; D020746:Calcium Channels, L-Type; D000451:Mineralocorticoid Receptor Antagonists; D012333:RNA, Messenger; D062687:Sodium Channel Agonists; D015222:Sodium Channels; D000450:Aldosterone