Magnesium lithospermate B ameliorates renal cortical microperfusion in rats.
OBJECTIVE
To investigate the effects of magnesium lithospermate B (MLB) isolated from Salviae miltiorrhizae on renal microcirculation, and renal and systemic hemodynamics in Sprague-Dawley rats.


METHODS
MLB (10, 30, and 60 mg/kg) was injected intravenously and renal blood flow (RBF), renal cortical microperfusion (RCM), and systemic hemodynamic function parameters including heart rate (HR), mean arterial pressure (MAP), left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), and maximal velocity of pressure increase (dp/dt(max)) were measured for 45 min after administration.


RESULTS
Intravenous MLB at doses of 10, 30, and 60 mg/kg increased RCM significantly, but had no obvious effects on RBF or systemic hemodynamics. The effect of MLB on RCM reached its peak 15 min after injection and returned to baseline after 45 min. Up to 60 mg/kg MLB increased RCM by 62.4%+/-20.2% (changes from baseline, P<0.01), whereas RBF (3.7%+/-9.7% vs baseline) and renal vascular resistance (-1.4%+/-9.1% vs baseline) did not obviously change.


CONCLUSIONS
These results indicate that MLB ameliorates renal microcirculation in a dose-dependent manner, which may be related to the renoprotective effects of MLB.
Chen
Chun-guang
CG
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.
Wang
Yi-ping
YP
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects*; D007275:Injections, Intravenous; D007672:Kidney Cortex / Q000098:blood supply* / Q000502:physiology; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012079:Renal Circulation / Q000187:drug effects*; D027561:Salvia miltiorrhiza / Q000737:chemistry; D014655:Vascular Resistance / Q000187:drug effects
D004365:Drugs, Chinese Herbal; D020011:Protective Agents; C061091:lithospermate B