title: Electrochemical detection of scDNA cleavage in the presence of macrocyclic hexaaza-copper(II) complex.
abstract: The hexaaza macrocyclic copper(II) complex Cu(II)L(L=1,8-Dihydroxyethyl-1,3,6,8,10,13-hexaazacyclotetradecane), which has octahedral structure similar to some natural complexes, is synthesized and purified. In this study, oxidative breakage DNA by the reaction of Cu(II)L with H2O2 and ascorbate has been investigated by gel electrophoresis experiments. In electrochemical experiments, the on scDNA-modified glassy carbon electrode(GCE) is cleaved by the Cu(II)L and redox changing of the metal catalyst without adding any other reagents. Above all, the need for concentration of scDNA is much lower than that of gel electrophoresis experiments and the process of the performance is easy. Furthermore, Cyclic Voltammetry (CV) and A.C. Impedance, which are performed to monitor scDNA cleavage at the scDNA-modified glassy carbon electrode (GCE), are fast, simple and highly efficient. The mechanism of the damage can be suggested: Fenton.
authors.affiliation: College Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, P.R. China. jwkang@nwnu.edu.cn
authors.forename: Jingwan
authors.initials: J
authors.lastname: Kang
authors.forename: Shuqing
authors.initials: S
authors.lastname: Dong
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Lu
authors.forename: Biquan
authors.initials: B
authors.lastname: Su
authors.forename: Haixia
authors.initials: H
authors.lastname: Wu
authors.forename: Kun
authors.initials: K
authors.lastname: Sun
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002384:Catalysis; D003300:Copper / Q000737:chemistry*; D004249:DNA Damage; D004278:DNA, Superhelical / Q000737:chemistry* / Q000187:drug effects; D004563:Electrochemistry; D004566:Electrodes; D006861:Hydrogen Peroxide / Q000737:chemistry; D047028:Macrocyclic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D010084:Oxidation-Reduction
chemical_list: C514681:1,8-dihydroxyethyl-1,3,6,8,10,13-hexaazacyclotetradecanecopper(II); D004278:DNA, Superhelical; D047028:Macrocyclic Compounds; D009942:Organometallic Compounds; D002244:Carbon; D003300:Copper; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid