Failure to genotype Human Cytomegalovirus by PCR-RFLP method due to sequence variation within the primer binding site.
Polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) is one of the rapid methods for genotyping Human Cytomegalovirus (HCMV). When genotyping clinical samples by a sensitive nested PCR-based RFLP method for the glycoprotein B (gB) gene of HCMV, it was found that some of the clinical specimens did not give an amplification signal. Analysis of the prototype sequences of the different genotypes showed base pair mismatches over the primer binding site. An alternative assay is suggested for genotyping of HCMV.
Sowmya
P
P
L and T Microbiology Research Centre, Vision Research Foundation, 18, College Road, Chennai 600006, India.
Madhavan
H N
HN
Therese
K L
KL
D001665:Binding Sites / Q000235:genetics; D003587:Cytomegalovirus / Q000145:classification / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000821:virology*; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000235:genetics
D017931:DNA Primers; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus