Inhibition of intestinal glucose uptake by aporphines and secoaporphines.
The effects of aporphines and secoaporphines on glucose uptake by isolated intestinal brush-border membrane vesicles (BBMV) or basolateral membrane vesicles (BLMV) and glucose absorption during in situ intestinal perfusion were studied. Of the tested compounds, N-allylsecoboldine was the most potent glucose uptake inhibitor, with IC50 values of 159 microM and 121 microM, respectively, for uptake by BBMV and BLMV. While thaliporphine competitively inhibited glucose uptake by both membrane preparations, inhibition by N-allylsecoboldine was competitive using BBMV and noncompetitive using BLMV. In addition, N-allylsecoboldine significantly reduced both glucose absorption during in situ intestinal perfusion and blood glucose levels in the oral glucose tolerance test. The results demonstrate that levels of both aporphines and secoaporphines achievable by oral administration have an inhibitory effect on intestinal glucose uptake and suggest that the hypoglycemic effects of these compounds merit attention.
Lin
Chun-Jung
CJ
School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
Chen
Chia-Hao
CH
Liu
Fu-Wen
FW
Kang
Jaw-Jou
JJ
Chen
Chien-Kuang
CK
Lee
Su-Lin
SL
Lee
Shoei-Sheng
SS
D000465:Algorithms; D000818:Animals; D001060:Aporphines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism*; D005951:Glucose Tolerance Test; D051273:Glucose Transporter Type 2 / Q000187:drug effects / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D022142:Secretory Vesicles / Q000378:metabolism; D051269:Sodium-Glucose Transporter 1 / Q000378:metabolism
D001060:Aporphines; D051273:Glucose Transporter Type 2; D051269:Sodium-Glucose Transporter 1; D005947:Glucose