title: Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information on a hyperthermophilic archaeon, Sulfolobus tokodaii strain 7.
abstract: sn-Glycerol-1-phosphate dehydrogenase is responsible for the formation of sn-glycerol-1-phosphate, the backbone of membrane phospholipids of Archaea. This activity had never been detected in cell-free extract of Sulfolobus sp. Here we report the detection of this activity on the thermostable ST0344 protein of Sulfolobus tokodaii expressed in Escherichia coli, which was predicted from genomic information on S. tokodaii. This is another line of evidence for the general mechanism of sn-glycerol-1-phosphate formation by the enzyme.
authors.affiliation: Department of Chemistry, School of Medicine, University of Occupational and Environmental Health, Japan. kogay@med.uoeh-u.ac.jp
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Koga
authors.forename: Mami
authors.initials: M
authors.lastname: Ohga
authors.forename: Masanari
authors.initials: M
authors.lastname: Tsujimura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morii
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawarabayasi
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D004795:Enzyme Stability; D015870:Gene Expression; D020745:Genome, Archaeal / Q000235:genetics*; D005993:Glycerolphosphate Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D016944:Sulfolobus / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D013696:Temperature
chemical_list: D005994:Glycerophosphates; D011994:Recombinant Proteins; D009249:NADP; D005993:Glycerolphosphate Dehydrogenase; C098215:glycerol-1-phosphate dehydrogenase