TLR9 activation is defective in common variable immune deficiency.
Common variable immune deficiency (CVID) is a primary immune deficiency characterized by low levels of serum immune globulins, lack of Ab, and reduced numbers of CD27+ memory B cells. Although T, B, and dendritic cell defects have been described, for the great majority, genetic causes have not been identified. In these experiments, we investigated B cell and plasmacytoid dendritic cell activation induced via TLR9, an intracellular recognition receptor that detects DNA-containing CpG motifs from viruses and bacteria. CpG-DNA activates normal B cells by the constitutively expressed TLR9, resulting in cytokine secretion, IgG class switch, immune globulin production, and potentially, the preservation of long-lived memory B cells. We found that CpG-DNA did not up-regulate expression of CD86 on CVID B cells, even when costimulated by the BCR, or induce production of IL-6 or IL-10 as it does for normal B cells. TLR9, found intracytoplasmically and on the surface of oligodeoxynucleotide-activated normal B cells, was deficient in CVID B cells, as was TLR9 mRNA. TLR9 B cell defects were not related to proportions of CD27+ memory B cells. CpG-activated CVID plasmacytoid dendritic cells did not produce IFN-alpha in normal amounts, even though these cells contained abundant intracytoplasmic TLR9. No mutations or polymorphisms of TLR9 were found. These data show that there are broad TLR9 activation defects in CVID which would prevent CpG-DNA-initiated innate immune responses; these defects may lead to impaired responses of plasmacytoid dendritic cells and loss of B cell function.
Cunningham-Rundles
Charlotte
C
Department of Medicine and the Immunobiology Center, Mount Sinai Medical Center, New York, NY 10029, USA. Charlotte.Cunningham-Rundles@MSSM.edu
Radigan
Lin
L
Knight
Adina K
AK
Zhang
Li
L
Bauer
Laura
L
Nakazawa
Atsushi
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D002462:Cell Membrane / Q000378:metabolism; D017074:Common Variable Immunodeficiency / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D018899:CpG Islands; D003713:Dendritic Cells; D005260:Female; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D016898:Interferon-alpha / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008875:Middle Aged; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000502:physiology; D051217:Toll-Like Receptor 9 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*
D016898:Interferon-alpha; D015850:Interleukin-6; D008565:Membrane Proteins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011947:Receptors, Antigen, B-Cell; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9; D016753:Interleukin-10