Molecular weight and subunit composition of the sensitive to GABA-ergic ligands Cl-/HCO3(-)-stimulated Mg2+-ATPase from plasma membrane of rat brain.
The molecular weight and subunit composition of Cl-,HCO3(-)- and picrotoxin-stimulated Mg2+-ATPase from rat brain plasma membrane solubilized in sodium deoxycholate were studied by gel filtration chromatography. The enzyme activity eluted from a Sephacryl S-300 column in a single peak associated with a protein of molecular weight approximately 300 kD and a Stokes radius of 5.4 nm. The enzyme-enriched fraction, concentrated and denatured by SDS, migrated through a Sephacryl S-200 column as three peaks with molecular weights of approximately 57, 53, and 45 kD. SDS-PAGE also showed three major protein bands with molecular weights of about 57, 53, and 48 kD. The molecular weight and subunit composition of the Cl- and HCO3(-)-stimulated Mg2+-ATPase from neuronal membrane of rat brain are similar with the molecular properties of GABA(A)-benzodiazepine receptor complex from mammalian brain but are different from those of P-type transport ATPases.
Menzikov
S A
SA
Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, 125315, Russia. menzikov@lansp.ru
Menzikova
O V
OV
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D001640:Bicuculline / Q000494:pharmacology; D001921:Brain / Q000201:enzymology*; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D002462:Cell Membrane / Q000201:enzymology; D002712:Chlorides / Q000494:pharmacology; D002850:Chromatography, Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D008970:Molecular Weight; D010852:Picrotoxin / Q000494:pharmacology; D021122:Protein Subunits / Q000737:chemistry*; D051381:Rats; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D001639:Bicarbonates; D001640:Bicuculline; D017301:Ca(2+) Mg(2+)-ATPase; D002712:Chlorides; D010852:Picrotoxin; D021122:Protein Subunits; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid