Effect of granulocyte macrophage-colony stimulating factor (GM-CSF) on 5-FU-induced ulcerative mucositis in hamster buccal pouches.
The present study was performed to investigate the protective effects of granulocyte macrophage-colony stimulating factor (GM-CSF) against ulcerative mucositis in hamster buccal pouch. GM-CSF was topically administered to the buccal pouches of hamsters with two different doses of 5 and 20 microg/ml. The treatment of GM-CSF led to rapid healing effects in gross and histopathological findings. It decreased expression of pro-inflammatory cytokine mRNA levels in the mucosal tissue of buccal pouches. Also GM-CSF-treated animals showed high numbers of Ki-67 positive cells in basal cell layer. These results suggest that GM-CSF provided excellent healing effects to ulcerative mucositis in the buccal pouch of hamster.
Cho
Sun-A
SA
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul 151-742, Republic of Korea.
Park
Jong-Hwan
JH
Seok
Seung-Hyeok
SH
Juhn
Jae-Hyeon
JH
Kim
Seong-Jin
SJ
Ji
Hyi-Jeong
HJ
Choo
Youn-Sung
YS
Park
Jae-Hak
JH
D000287:Administration, Topical; D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D006224:Cricetinae; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005472:Fluorouracil / Q000633:toxicity*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000627:therapeutic use*; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019226:Oral Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D011994:Recombinant Proteins; D013280:Stomatitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology
D000897:Anti-Ulcer Agents; D015415:Biomarkers; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005472:Fluorouracil