Daidzein activates choline acetyltransferase from MC-IXC cells and improves drug-induced amnesia.
The choline acetyltransferase (ChAT) activator, which enhances cholinergic transmission via an augmentation of the enzymatic production of acetylcholine (ACh), is an important factor in the treatment of Alzheimer's disease (AD). Methanolic extracts from Pueraria thunbergiana exhibited an activation effect (46%) on ChAT in vitro. Via the sequential isolation of Pueraria thunbergiana, the active component was ultimately identified as daidzein (4',7-dihydroxy-isoflavone). In order to investigate the effects of daidzein from Pueraria thunbergiana on scopolamine-induced impairments of learning and memory, we conducted a series of in vivo tests. Administration of daidzein (4.5 mg/kg body weight) to mice was shown significantly to reverse scopolamine-induced amnesia, according to the results of a Y-maze test. Injections of scopolamine into mice resulted in impaired performance on Y-maze tests (a 37% decreases in alternation behavior). By way of contrast, mice treated with daidzein prior to the scopolamine injections were noticeably protected from this performance impairment (an approximately 12%-21% decrease in alternation behavior). These results indicate that daidzein might play a role in acetylcholine biosynthesis as a ChAT activator, and that it also ameliorates scopolamine-induced amnesia.
Heo
Ho Jin
HJ
Jeonnam Innovation Agency, Jeonnam, Korea.
Suh
Young-Min
YM
Kim
Mi-Jeong
MJ
Choi
Soo-Jung
SJ
Mun
Nam Shik
NS
Kim
Hye-Kyung
HK
Kim
Eunki
E
Kim
Chang-Ju
CJ
Cho
Hong-Yon
HY
Kim
Young Jun
YJ
Shin
Dong-Hoon
DH
D000647:Amnesia / Q000139:chemically induced* / Q000188:drug therapy* / Q000201:enzymology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002460:Cell Line; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology; D029908:Pueraria / Q000737:chemistry; D012601:Scopolamine / Q000494:pharmacology; D013662:Tea / Q000737:chemistry
D007529:Isoflavones; D010936:Plant Extracts; D013662:Tea; C004742:daidzein; D012601:Scopolamine; D002795:Choline O-Acetyltransferase