Behaviors of enzyme immobilization onto functional microspheres.
Micron-grade monodisperse PMMA microspheres, whose surfaces were modified with functional groups by co-polymerisation using functional monomer, were prepared via dispersion polymerisation. Characterized by their large specific surface area, high adsorption ability, favourable biocompatibility, these monodisperse micron-sized PMMA microspheres were employed as the supporting material in the enzyme immobilization in present work. The influential factors on the activity of immobilized enzyme including pH, temperature, time etc were preliminarily investigated. The results concluded from the experiments indicated that the immobilization procedure could promote the resistance of enzyme against temperature, pH shift and some other tough reaction conditions meanwhile prolong the enzymatic lifetime for storage.
Wu
Shaogui
S
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, China.
Liu
Bailing
B
Li
Songjun
S
D004800:Enzymes, Immobilized / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008863:Microspheres; D013696:Temperature; D013997:Time Factors; D014357:Trypsin / Q000502:physiology*
D004800:Enzymes, Immobilized; D014357:Trypsin