DNA barcoding of blastocystis.
We have developed a simple method for subtyping the intestinal protistan parasite Blastocystis using an approach equivalent to DNA barcoding in animals. Amplification of a 600 bp region of the small subunit ribosomal RNA gene followed by single primer sequencing of the PCR product provides enough data to assign isolates to specific subtypes unambiguously. We believe that this approach will prove useful in future epidemiological studies.
Scicluna
Stephanie M
SM
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
Tawari
Blessing
B
Clark
C Graham
CG
D000818:Animals; D016844:Blastocystis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016776:Blastocystis Infections / Q000469:parasitology*; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis* / Q000235:genetics; D020459:Genes, rRNA / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal