Jannaschia seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea.
A slightly halophilic alphaproteobacterium, designated CL-SP26T, was isolated from hypersaline water of a solar saltern located in Seosin, Korea. 16S rRNA gene sequence analysis revealed an affiliation with the genus Jannaschia. Sequence similarities between CL-SP26T and type strains of members of the genus Jannaschia were 94.9-97.2 %. Cells were rod-shaped and motile with one or more monopolar flagella. Strain CL-SP26T grew on solid media as circular red colonies. It was able to grow in 3-10 % sea salt; however, no growth occurred in media containing NaCl as the only salt. Strain CL-SP26T grew at 5-35 degrees C and pH 7-10. The major fatty acids were 18 : 1omega7c (64 %) and 18 : 0 (12.0 %). Three fatty acids, 3-OH 14 : 0/iso 16 : 1 (3.6 %), 18 : 3omega6c (2.2 %) and 10-methyl 19 : 0 (1.9 %), found in minor quantities in CL-SP26(T), are unique among Jannaschia species. The DNA G+C content was 63 mol%. According to physiological data, fatty acid composition and 16S rRNA gene sequence analysis, CL-SP26T was assigned to the genus Jannaschia, but could be distinguished from recognized species of the genus. Strain CL-SP26T (= KCCM 42114T = JCM 13035T) therefore represents a novel species, for which the name Jannaschia seosinensis sp. nov. is proposed.
Choi
Dong Han
DH
School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Yi
Hana
H
Chun
Jongsik
J
Cho
Byung Cheol
BC
D020561:Alphaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D003470:Culture Media; D005227:Fatty Acids; D006875:Hydrotherapy; D007723:Korea; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts; D012623:Seawater / Q000382:microbiology*; D017421:Sequence Analysis; D013045:Species Specificity
D003470:Culture Media; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012492:Salts