Antimicrobial activity of glycosidase inhibitory protein isolated from Cyphomandra betacea Sendt. fruit.
Broad-spectrum antimicrobial activity of an invertase inhibitory protein (IIP) isolated from Cyphomandra betacea ripe fruits is documented. Minimal inhibitory concentration (MIC) values were determined by agar macrodilution and broth microdilution assays. This IIP inhibited the growth of xylophagous and phytopatogenic fungi (Ganoderma applanatum, Schizophyllum commune, Lenzites elegans, Pycnoporus sanguineous, Penicillium notatum, Aspergillus niger, Phomopsis sojae and Fusarium mango) and phytopathogenic bacteria (Xanthomonas campestris pvar vesicatoria CECT 792, Pseudomonas solanacearum CECT 125, Pseudomonas corrugata CECT 124, Pseudomonas syringae pv. syringae and Erwinia carotovora var carotovora). The IIP concentration required to completely inhibit the growth of all studied fungi ranged from 7.8 to 62.5 microg/ml. Phytopatogenic bacteria were the most sensitive, with MIC values between 7.8 and 31.25 microg/ml. Antifungal and antibacterial activities can be associated with their ability to inhibit hydrolytic enzymes. Our results indicate the possible participation of IIP in the plant defense mechanism and its potential application as a biocontrol agent against phytopathogenic fungi and bacteria.
Ordóñez
Roxana M
RM
Cátedra de Fitoquímica, Instituto de Estudios Vegetales Dr. Antonio R. Sampietro, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 461, 4000-San Miguel de Tucumán, Tucumán, Argentina.
Ordóñez
Adriana A L
AA
Sayago
Jorge E
JE
Nieva Moreno
María I
MI
Isla
María I
MI
D000890:Anti-Infective Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000378:metabolism; D005638:Fruit / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors*; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry
D000890:Anti-Infective Agents; D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D010455:Peptides; D010940:Plant Proteins; D006026:Glycoside Hydrolases