Safety assessment of potential lactic acid bacteria Bifidobacterium longum SPM1205 isolated from healthy Koreans.
The safety assessment of Bifidobacterium longum SPM1205 isolated from healthy Koreans and this strain's inhibitory effects on fecal harmful enzymes of intestinal microflora were investigated. The overall safety of this strain was investigated during a feeding trial. Groups of SD rats were orally administered a test strain or commercial reference strain B. longum 1 x 10(9) CFU/kg body weight/day for four weeks. Throughout this time, their feed intake, water intake and live body weight were monitored. Fecal samples were periodically collected to test harmful enzyme activities of intestinal microflora. At the end of the four-week observation period, samples of blood, liver, spleen, kidney, and gut tissues were collected to determine for hematological parameters and histological differences. The results obtained in this experiment demonstrated that four weeks of consumption of this Bifidobacterium strain had no adverse effects on rat's general health status, blood biochemical parameters or histology. Therefore, it is likely to be safe for human use. Fecal harmful enzymes such as beta-glucosidase, beta-glucuronidase, tryptophanase and urease, were effectively inhibited during the administration of the B. longum SPM1205. These results suggested that this B. longum SPM 1205 could be used for humans as a probiotic strain.
Choi
Sung Sook
SS
Department of Food Sciencs, Sahmyook College, Gyeonggi-do, Republic of Korea.
Kang
Byung Yong
BY
Chung
Myung Jun
MJ
Kim
Soo Dong
SD
Park
So Hee
SH
Kim
Jung Soo
JS
Kang
Chin Yang
CY
Ha
Nam Joo
NJ
D000328:Adult; D000818:Animals; D001483:Base Sequence; D001644:Bifidobacterium / Q000302:isolation & purification* / Q000502:physiology*; D001773:Blood Cells; D001774:Blood Chemical Analysis; D001835:Body Weight; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D004326:Drinking; D004435:Eating; D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000382:microbiology; D005966:Glucuronidase / Q000037:antagonists & inhibitors; D006801:Humans; D007723:Korea; D008969:Molecular Sequence Data; D019936:Probiotics / Q000008:administration & dosage* / Q000009:adverse effects*; D051381:Rats; D014368:Tryptophanase / Q000037:antagonists & inhibitors; D014510:Urease / Q000037:antagonists & inhibitors; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D001617:beta-Glucosidase; D005966:Glucuronidase; D014510:Urease; D014368:Tryptophanase