title: Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil.
abstract: The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.
authors.affiliation: Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, SP, Brazil.
authors.forename: Cristina Pires de
authors.initials: CP
authors.lastname: Araújo
authors.forename: Clarice Queico Fugimura
authors.initials: CQ
authors.lastname: Leite
authors.forename: Karina Andrade de
authors.initials: KA
authors.lastname: Prince
authors.forename: Klaudia dos Santos Gonçalves
authors.initials: Kdos S
authors.lastname: Jorge
authors.forename: Ana Luiza Alves Rosa
authors.initials: AL
authors.lastname: Osório
mesh_terms: D000003:Abattoirs; D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D001938:Brazil; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D008460:Meat / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014380:Tuberculosis, Bovine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial