title: Protective effect of donepezil on retinal ganglion cells in vitro and in vivo.
abstract: The neuroprotective effect of donepezil, an acetylcholinesterase inhibitor on retinal ganglion cells (RGCs), was examined. The survival of purified RGCs after exposure to glutamate with or without donepezil (10(- 7) M to 10(- 5) M) was measured after 3 days in culture. In vivo, the neuroprotective effect of donepezil was examined by quantifying the number of viable RGCs 7 days after axotomy in adult rats. In vitro, donepezil dose-dependently reduced RGC death caused by glutamate toxicity. Oral administration of donepezil (10 mg kg(-1) day(-1)) significantly reduced RGC death after axotomy. In conclusion, donepezil exerts a protective effect on RGCs both in vitro and in vivo.
authors.affiliation: Department of Ophthalmology and Visual Science, Osaka University Medical School, Suita, Osaka, Japan.
authors.forename: Atsuya
authors.initials: A
authors.lastname: Miki
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Otori
authors.forename: Takeshi
authors.initials: T
authors.lastname: Morimoto
authors.forename: Masaki
authors.initials: M
authors.lastname: Okada
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tano
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019771:Axotomy; D001578:Benzopyrans / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D018680:Cholinergic Antagonists / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D019610:Cytoprotection; D000077265:Donepezil; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D018698:Glutamic Acid / Q000633:toxicity; D007189:Indans / Q000494:pharmacology*; D008464:Mecamylamine / Q000494:pharmacology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009570:Nitriles / Q000494:pharmacology; D020221:Optic Nerve Injuries / Q000473:pathology; D010880:Piperidines / Q000494:pharmacology*; D051381:Rats; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000473:pathology; D012601:Scopolamine / Q000494:pharmacology
chemical_list: D001578:Benzopyrans; D018680:Cholinergic Antagonists; D002800:Cholinesterase Inhibitors; D004791:Enzyme Inhibitors; D007189:Indans; D018696:Neuroprotective Agents; D009570:Nitriles; D010880:Piperidines; C456800:ethyl 2-amino-6-bromo-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate; D018698:Glutamic Acid; D008464:Mecamylamine; D000077265:Donepezil; D012601:Scopolamine