title: Age-dependent interactive changes in serotonergic and noradrenergic cortical axon terminals in F344 rats.
abstract: In the frontal cortex of aging rats, we found an increase in sprouting of the noradrenergic (NA) axons originated from the locus coeruleus (LC). The serotonergic (5-HT) axons originating from the dorsal raphe (DR) share the same cortical area and their age-dependent changes and interactions with NA axons were still unclear. To compare quantitatively the extent of axonal sprouting of DR and LC neurons in the frontal cortex, we extracellularly recorded from both DR and LC neurons in the same animals and antidromically stimulated 32 cortical sites (a pair of stimulating electrodes was moved at 100-mum intervals from 500 to 2000 microm in depth). In addition, to examine the effects of degeneration of 5-HT axons on NA axons, and vice versa, we used specific neurotoxins for 5-HT (PCA) or NA (DSP-4) axons. We also used noradrenaline uptake inhibitor (maprotiline) to verify the effects of NA on degeneration of 5-HT axons. Results suggested that 5-HT axons sprouted between 15 and 17 months of age and noradrenaline accelerated the age-dependent change of 5-HT axons.
authors.affiliation: Department of Basic Gerontology, National Center for Geriatrics and Gerontology, 36-3 Gengo, Morioka, Obu, Aichi 474-8522, Japan.
authors.forename: Sadamu
authors.initials: S
authors.lastname: Nakai
authors.forename: Wataru
authors.initials: W
authors.lastname: Matsunaga
authors.forename: Kenichi
authors.initials: K
authors.lastname: Isobe
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Shirokawa
mesh_terms: D018759:Adrenergic Uptake Inhibitors / Q000494:pharmacology; D000375:Aging; D000818:Animals; D001596:Benzylamines / Q000494:pharmacology; D005625:Frontal Lobe / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008376:Maprotiline / Q000494:pharmacology; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D012701:Serotonin / Q000378:metabolism*
chemical_list: D018759:Adrenergic Uptake Inhibitors; D001596:Benzylamines; D014179:Neurotransmitter Uptake Inhibitors; D008376:Maprotiline; D012701:Serotonin; C012102:DSP 4; D009638:Norepinephrine