Extracellular adenine nucleotides inhibit the release of major monocyte recruiters by human monocyte-derived dendritic cells.
Extracellular ATP is known to affect the maturation of monocyte-derived dendritic cells mainly by regulation of cytokines and costimulatory molecules. The present study describes the inhibition of MCP-1 (CCL2) and MIP-1alpha (CCL3) release by human monocyte-derived dendritic cells in response to adenine nucleotides. Our pharmacological data support the involvement of P2Y11 and P2Y1 purinergic receptors in the downregulation of these major monocyte recruiters. Migration assays have demonstrated that supernatants of dendritic cells treated with adenine nucleotides or anti-MCP-1/MIP-1alpha blocking antibodies display a strongly reduced capacity to attract monocytes and immature dendritic cells.
Horckmans
Michael
M
Institute of Interdisciplinary Research, IRIBHM - Faculty of Medicine, Université Libre de Bruxelles, 808 Route de Lennik, Campus Erasme, Building C 5th Floor, 1070 Brussels, Belgium.
Marcet
Brice
B
Marteau
Frédéric
F
Bulté
Frédéric
F
Maho
Arielle
A
Parmentier
Marc
M
Boeynaems
Jean-Marie
JM
Communi
Didier
D
D000227:Adenine Nucleotides / Q000276:immunology / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000276:immunology*; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D002633:Chemotaxis / Q000187:drug effects* / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D019402:Macrophage Inflammatory Proteins / Q000276:immunology*; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D018048:Receptors, Purinergic P2 / Q000276:immunology; D058472:Receptors, Purinergic P2Y1
D000227:Adenine Nucleotides; D000911:Antibodies, Monoclonal; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins; C546382:P2RY1 protein, human; C109734:P2RY11 protein, human; D018048:Receptors, Purinergic P2; D058472:Receptors, Purinergic P2Y1