title: Survival and proliferative ability of various living cell types after laser-induced forward transfer.
abstract: The survival, proliferation, and differentiation of freshly isolated and cultured cells were studied after absorbing film-assisted laser-induced forward transfer. Rat Schwann and astroglial cells and pig lens epithelial cells were used for transfer and the cells were cultured for 2 weeks after laser-pulsed transfer. All three cell types survived, proliferated, and differentiated under cell culture conditions and regained their original phenotype a few days after cell transfer. Time resolution studies have shown that the time required to accelerate the jets and droplets containing the cells was less than 1 micros and that the estimated minimum average acceleration of those ejected cells that reached a constant velocity was approximately 10(7) x g. This suggests that the majority of studied cells tolerated the extremely high acceleration at the beginning of the ejection and the deceleration during impact on the acceptor plate without significant damage to the original phenotype. These results suggest that the absorbing film-assisted laser-induced forward transfer technique appears to be suitable for several potential applications in tissue engineering and the biomedical tissue repair technologies.
authors.affiliation: Research Group on Laser Physics, Hungarian Academy of Sciences and University of Szeged, Szeged, Hungary. bhopp@physx.u-szeged.hu
authors.forename: Béla
authors.initials: B
authors.lastname: Hopp
authors.forename: Tomi
authors.initials: T
authors.lastname: Smausz
authors.forename: Norbert
authors.initials: N
authors.lastname: Kresz
authors.forename: Norbert
authors.initials: N
authors.lastname: Barna
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Bor
authors.forename: Lajos
authors.initials: L
authors.lastname: Kolozsvári
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Chrisey
authors.forename: András
authors.initials: A
authors.lastname: Szabó
authors.forename: Antal
authors.initials: A
authors.lastname: Nógrádi
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D007834:Lasers; D007908:Lens, Crystalline / Q000166:cytology / Q000502:physiology*; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000502:physiology*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods