title: Engineered membrane superchannel improves bioremediation potential of dioxin-degrading bacteria.
abstract: Sphingomonas sp. A1 possesses specialized membrane structures termed 'superchannels' that enable the direct incorporation of macromolecules into the cell. We have engineered two related sphingomonads, the dioxin-degrading S. wittichii RW1 and the polypropylene glycol-degrading S. subarctica IFO 16058(T), to incorporate this superchannel into their cell membranes. In both cases the bioremediation capability of the organisms was substantially increased pointing at the potential of this approach as a general strategy to improve bacterial degradation of hazardous compounds in the environment.
authors.affiliation: Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Aso
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: Karen Mine
authors.initials: KM
authors.lastname: Harada
authors.forename: Keiko
authors.initials: K
authors.lastname: Momma
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kawai
authors.forename: Wataru
authors.initials: W
authors.lastname: Hashimoto
authors.forename: Bunzo
authors.initials: B
authors.lastname: Mikami
authors.forename: Kousaku
authors.initials: K
authors.lastname: Murata
mesh_terms: D001673:Biodegradation, Environmental; D004147:Dioxins / Q000302:isolation & purification / Q000493:pharmacokinetics*; D024861:Genetic Enhancement / Q000379:methods*; D007220:Industrial Waste / Q000517:prevention & control; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D020578:Sphingomonas / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004147:Dioxins; D007220:Industrial Waste; D007473:Ion Channels; D011994:Recombinant Proteins