title: Mutation in the Sp1 motif of the bovine leptin gene affects its expression.
abstract: Leptin is expressed mainly by adipocytes and plays a crucial role in the regulation of energy expenditure, food intake, and adiposity. Using PCR-heteroduplex analysis and sequencing, we investigated a C/G substitution in the promoter region of the bovine leptin gene. Application of the electrophoretic mobility shift assay showed that the C-->G transversion decreased the leptin gene promoter binding capacity for nuclear proteins. With real-time PCR and Western blotting, we showed that the leptin expression level was higher in cattle with the CC than with the GG genotype.
authors.affiliation: Department of Genetics and Animal Breeding, Agricultural University of Poznan, Poznan, Poland.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Adamowicz
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Flisikowski
authors.forename: Rafał
authors.initials: R
authors.lastname: Starzyński
authors.forename: Lech
authors.initials: L
authors.lastname: Zwierzchowski
authors.forename: Marek
authors.initials: M
authors.lastname: Switoński
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D015342:DNA Probes; D005786:Gene Expression Regulation / Q000235:genetics*; D020738:Leptin / Q000235:genetics*; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor / Q000235:genetics*
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D020738:Leptin; D016329:Sp1 Transcription Factor