Structures of the core oligosaccharide and O-units in the R- and SR-type lipopolysaccharides of reference strains of Pseudomonas aeruginosa O-serogroups.
Highly phosphorylated core oligosaccharides and those substituted with one O-antigen repeating unit were obtained by mild acid degradation or strong alkaline hydrolysis of lipopolysaccharide samples from 23 reference strains representing all Pseudomonas aeruginosa O-serogroups. Studies by high-resolution electrospray ionization mass spectrometry and two-dimensional NMR spectroscopy revealed both conserved and variable structural features of the lipopolysaccharides of various O-serogroups. The upstream terminal saccharide of the O-antigen, which contributes most to the immunospecificity of the bacteria, was defined in 11 from a total of 13 O-serogroups. The data obtained link together the known biosynthesis pathways, genetics and serology of the P. aeruginosa lipopolysaccharide.
Bystrova
Olga V
OV
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. olgabv@ioc.ac.ru
Knirel
Yuriy A
YA
Lindner
Buko
B
Kocharova
Nina A
NA
Kondakova
Anna N
AN
Zähringer
Ulrich
U
Pier
Gerald B
GB
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D021241:Spectrometry, Mass, Electrospray Ionization
D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides