title: The efficiency of interference of Potato virus X infection depends on the target gene.
abstract: RNA silencing is a natural defense response against viral infection. This phenomenon has been used to interfere with viral infections by exploiting fragments of viral genomes as sources of RNA silencing. Agrobacterium-mediated transient expression of a hairpin RNA derived from the TGBp1 gene of Potato virus X (PVX) induced RNA silencing of the TGBp1 gene and resulted in interference of PVX infection. The interference was induced in the infiltrated leaves but not in the upper non-infiltrated leaves. Transient expression of a CP hairpin RNA also induced interference of PVX. The TGBp1 hairpin RNA showed more efficient interference of PVX infection than the CP hairpin RNA.
authors.affiliation: Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Takahashi
authors.forename: Ken
authors.initials: K
authors.lastname: Komatsu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kagiwada
authors.forename: Johji
authors.initials: J
authors.lastname: Ozeki
authors.forename: Takuma
authors.initials: T
authors.lastname: Mori
authors.forename: Hisae
authors.initials: H
authors.lastname: Hirata
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Yamaji
authors.forename: Masashi
authors.initials: M
authors.lastname: Ugaki
authors.forename: Shigetou
authors.initials: S
authors.lastname: Namba
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D017863:Potexvirus / Q000235:genetics / Q000254:growth & development*; D034622:RNA Interference; D012367:RNA, Viral / Q000096:biosynthesis; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins