title: [Protective effect of ischemic postconditioning on ischemic reperfusion injury of rat liver graft].
abstract: OBJECTIVE
  To observe the protective effect of ischemic postconditioning on ischemic reperfusion injury of rat liver graft and to investigate the possible mechanism.
  
  
  METHODS
  Male Sprague Dawley rats were used as donors and recipients of orthotopic liver transplantation, and the period of cold preservation and anhepatic phase were 100 min and 18 min, respectively. Sixty rats were randomly divided into three groups, twelve rats in control group, twenty-four rats in ischemic reperfusion injury group and ischemic postconditioning group respectively. Control group is sham operation group, only the ligaments around liver were cut off; donor livers in ischemic reperfusion injury group were infused through portal vein with heparinized saline before harvested; ischemic postconditioning group: at very onset of reperfusion after donor liver was implanted, several brief reperfusion-ischemia were given before persistent reperfusion of portal vein. Half recipients of ischemic reperfusion injury group and ischemic postconditioning group were taken blood samples and hepatic tissue samples after 2 hours of reperfusion of liver graft. Rest recipients were taken samples of hepatic tissue after 6 hours of reperfusion. Recipients of control group were taken blood and hepatic tissue samples at corresponding time after abdomen was sutured.
  
  
  RESULTS
  Compared with ischemic reperfusion injury group, liver functional parameters, cytokines and peroxidized products contents were lower in ischemic postconditioning group (P < 0.05); meanwhile, the antioxidases contents of hepatic tissue were higher in ischemic postconditioning group than those in ischemic reperfusion injury group (P < 0.05).
  
  
  CONCLUSIONS
  Ischemic postconditioning could relieve the ischemic reperfusion injury of rat liver graft. Through improving antioxidation capability and cutting down cytokines contents, ischemic postconditioning could apply its protective effect.
authors.affiliation: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. wangnandoc2002@yahoo.com.cn
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Qing-jiu
authors.initials: QJ
authors.lastname: Ma
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Lu
authors.forename: Yan-kui
authors.initials: YK
authors.lastname: Chu
authors.forename: Da-nian
authors.initials: DN
authors.lastname: Lai
mesh_terms: D000818:Animals; D005979:Glutathione Peroxidase / Q000378:metabolism; D019272:Leukocyte Elastase / Q000097:blood; D008099:Liver / Q000098:blood supply* / Q000378:metabolism; D016031:Liver Transplantation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D019272:Leukocyte Elastase