title: [Research on Culex tritaeniorhynchus and Culex pipiens quinquefasciatus intrathoracically infected with attenuated Japanese encephalitis virus SA14-14-2 vaccine strain].
abstract: BACKGROUND
  To determine if the attenuated Japanese encephalitis (JE) virus SA14-14-2 vaccine strain interacts efficiently with Culex tritaeniorhynchus and Culex pipiens quinquefasciatus, and further to acquire a new knowledge of its characteristics and safety for human beings.
  
  
  METHODS
  Laboratory colonies of the two species of mosquitoes were set up and were inoculated intrathoracically with the attenuated vaccine virus and wild JE virus (Nak), both of which were used with different dilution from 10(-1) to 10(-9). Subsequently, the virus titers in the mosquitoes were detected by the plaque assay.
  
  
  RESULTS
  Inoculated with the vaccine strain, two species of mosquitoes were infected with the titers ranged from 10(0)-10(-3), and the maximum titers in Culex tritaeniorhynchus and Culex pipiens quinquefasciatus were 4.48 logPFU/ml and 5.63 logPFU/ml, respectively. Inoculated with wild JE virus, Culex pipiens quinquefasciatus was infected with titers ranged from 10(0)-10(-5), and the maximum titer in the mosquitoes was 6.59; Culex tritaeniorhynchus was infected with titers ranged from 10(0)-10(-4) and the maximum titer was 5.74 logPFU/ml.
  
  
  CONCLUSIONS
  By intrathoracic infection, the attenuated JE virus SA14-14-2 vaccine strain can replicate in both species of mosquitoes.
authors.affiliation: Yunnan Institute of Endemic Diseases Control and Prevention, Dali 671000, China.
authors.forename: Yu-zheng
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Hai-lin
authors.initials: HL
authors.lastname: Zhang
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Yu
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.forename: Guan-mu
authors.initials: GM
authors.lastname: Dong
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Yang
authors.forename: Li-li
authors.initials: LL
authors.lastname: Jia
authors.forename: Ya-fu
authors.initials: YF
authors.lastname: Yao
mesh_terms: D000818:Animals; D003465:Culex / Q000145:classification / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D004672:Encephalitis, Japanese / Q000821:virology; D006801:Humans; D007303:Insect Vectors / Q000821:virology*; D022321:Japanese Encephalitis Vaccines / Q000276:immunology; D013045:Species Specificity; D014613:Vaccines, Attenuated / Q000276:immunology; D010948:Viral Plaque Assay
chemical_list: D022321:Japanese Encephalitis Vaccines; D014613:Vaccines, Attenuated