title: Genomic analysis of a 1 Mb region near the telomere of Hessian fly chromosome X2 and avirulence gene vH13.
abstract: BACKGROUND
  To have an insight into the Mayetiola destructor (Hessian fly) genome, we performed an in silico comparative genomic analysis utilizing genetic mapping, genomic sequence and EST sequence data along with data available from public databases.
  
  
  RESULTS
  Chromosome walking and FISH were utilized to identify a contig of 50 BAC clones near the telomere of the short arm of Hessian fly chromosome X2 and near the avirulence gene vH13. These clones enabled us to correlate physical and genetic distance in this region of the Hessian fly genome. Sequence data from these BAC ends encompassing a 760 kb region, and a fully sequenced and assembled 42.6 kb BAC clone, was utilized to perform a comparative genomic study. In silico gene prediction combined with BLAST analyses was used to determine putative orthology to the sequenced dipteran genomes of the fruit fly, Drosophila melanogaster, and the malaria mosquito, Anopheles gambiae, and to infer evolutionary relationships.
  
  
  CONCLUSIONS
  This initial effort enables us to advance our understanding of the structure, composition and evolution of the genome of this important agricultural pest and is an invaluable tool for a whole genome sequencing effort.
authors.affiliation: Indiana Center for Insect Genomics, University of Notre Dame, Notre Dame, Indiana 46556, USA. nlobo@nd.edu
authors.forename: Neil F
authors.initials: NF
authors.lastname: Lobo
authors.forename: Susanta K
authors.initials: SK
authors.lastname: Behura
authors.forename: Rajat
authors.initials: R
authors.lastname: Aggarwal
authors.forename: Ming-Shun
authors.initials: MS
authors.lastname: Chen
authors.forename: Frank H
authors.initials: FH
authors.lastname: Collins
authors.forename: Jeff J
authors.initials: JJ
authors.lastname: Stuart
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D004175:Diptera / Q000235:genetics* / Q000472:pathogenicity; D005260:Female; D049750:Genome, Insect; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D016615:Telomere / Q000235:genetics*; D014908:Triticum / Q000469:parasitology; D014960:X Chromosome