Cadmium-induced genotoxicity, cytotoxicity and lipid peroxidation in Allium sativum and Vicia faba.
Cadmium (Cd) is one of the most toxic environmental pollutants affecting cytogenetically the various organisms. The cytogenetic damage in root tip cells exposed to cadmium nitrate (CdNO3) solutions at four different concentrations (1, 10, 100 and 200 microM) was evaluated with biological tests based on micronucleus (MN) assay in two plant species, Allium sativum and Vicia faba. Additionally to the cytogenetic analysis, lipid peroxidation analyses were performed in both A.sativum and V.faba roots. Cd enhanced the MN frequency in both A.sativum and V.faba root tip cells, but no dose-dependent. Induction of MN is not depending on CdNO3 concentrations. Besides, high concentrations of Cd decreased the mitotic index and caused the delay in mitosis stages in both plants, mainly in V.faba. On the other hand, lipid peroxidation was significantly enhanced with external Cd in V.faba. The results clearly indicate that high concentrations of cadmium induce the lipid peroxidation resulting in oxidative stress that may contribute to the genotoxicity and cytotoxicity of Cd ions.
Unyayar
Serpil
S
Department of Biology, Faculty of Science and Art, Mersin University, 33342 Mersin, Turkey.
Celik
Ayla
A
Cekiç
F Ozlem
FO
Gözel
Aysin
A
D002104:Cadmium / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects*; D005737:Garlic / Q000187:drug effects* / Q000235:genetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D015162:Micronucleus Tests; D008938:Mitosis / Q000187:drug effects; D018384:Oxidative Stress; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D031307:Vicia faba / Q000187:drug effects* / Q000235:genetics
D002104:Cadmium