title: Immune responses induced in cattle by vaccination with a recombinant adenovirus expressing Mycobacterial antigen 85A and Mycobacterium bovis BCG.
abstract: Cattle were vaccinated with an adenovirus expressing the mycobacterial antigen 85A (rAd85A), with Mycobacterium bovis BCG followed by rAd85A heterologous boosting, or with rAd85A followed by BCG boosting. BCG/rAd85A resulted in the highest direct gamma interferon responses. Cultured enzyme-linked immunospot assay analysis demonstrated that memory responses were induced by all three protocols but were strongest after BCG/rAd85A and rAd85A/BCG vaccination.
authors.affiliation: TB Research Group, VLA-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. m.vordermeier@vla.defra.gsi.gov.uk
authors.forename: H Martin
authors.initials: HM
authors.lastname: Vordermeier
authors.forename: Kris
authors.initials: K
authors.lastname: Huygen
authors.forename: Mahavir
authors.initials: M
authors.lastname: Singh
authors.forename: R Glyn
authors.initials: RG
authors.lastname: Hewinson
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xing
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D001500:BCG Vaccine / Q000008:administration & dosage* / Q000276:immunology*; D002417:Cattle; D007115:Immunization Schedule; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D009163:Mycobacterium bovis / Q000276:immunology*; D014380:Tuberculosis, Bovine / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D001500:BCG Vaccine; C065036:antigen 85, Mycobacterium bovis; D007371:Interferon-gamma