Ultrasensitive human immunodeficiency virus type 1 p24 antigen assay modified for use on dried whole-blood spots as a reliable, affordable test for infant diagnosis.
The ultrasensitive human immunodeficiency virus (HIV) p24 antigen assay was modified for use on pediatric dried whole-blood spots on Whatman no. 1 filter paper. The modified assay was found to be reliable and accurate, making it an affordable tool for pediatric HIV diagnosis in developing countries.
Patton
Janet C
JC
Wits Paediatric HIV Unit, Wits Health Consortium, University of the Witwatersrand, 7 York Rd., Parktown 2193, Johannesburg, South Africa. janet.patton@nhls.ac.za
Sherman
Gayle G
GG
Coovadia
Ashraf H
AH
Stevens
Wendy S
WS
Meyers
Tammy M
TM
D001774:Blood Chemical Analysis; D003890:Desiccation; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics* / Q000379:methods; D016655:HIV Core Protein p24 / Q000097:blood*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D015997:Neonatal Screening; D012680:Sensitivity and Specificity
D016655:HIV Core Protein p24