title: Quantitative studies of liver atrophy after portacaval shunt in the rat.
abstract: BACKGROUND
  It is well known that portacaval shunting ultimately leads to a decrease in liver volume and hepatic function, but the mechanism is uncertain. The aim of the present study was to evaluate the effect of portacaval shunting (PCS) upon the morphological changes that occur in the liver in rats after port caval anastomosis.
  
  
  METHODS
  Sixty-six male rats underwent either PCS (n = 35) or sham operations (n = 31). Hormone levels were determined in blood samples taken just before removal and weighing of the livers. Hematoxylin and eosin-stained sections were used for quantitative morphometric analysis. Apoptosis, mitosis, and cellular organelles also were assessed quantitatively.
  
  
  RESULTS
  There was a significant reduction in the liver mass together with testosterone levels in PCS rats in comparison with sham rats. The distance between presinusoidal and postsinusoidal vessels was reduced from 500 mum in the sham rats to 299 mum in the PCS rats (P = 0.000001). Within the same group, there was a significant reduction in the area of hepatocyte nuclei in zone 3 in comparison with zone 1. Electron microscopy revealed a highly significant (P = 0.0007) reduction in the membrane-bound cytoplasmic organelles of zone 3 hepatocytes in PCS rats in comparison with the sham rats. Apoptosis was increased in zone 3 in PCS rats (P = 0.00001), whereas in zone 1 of the same group, there was an associated increased in mitosis (P = 0.000001). Overall, the degree of apoptosis was in excess of mitosis, resulting in a general loss of liver mass.
  
  
  CONCLUSIONS
  Morphometric analysis at cellular and subcellular levels confirms the morphological findings of liver atrophy in PCS rats. The mechanism of atrophy is a complex one. Portacaval shunting leads to hepatic atrophy that, in turn, results in microcirculatory and hormonal changes that further contribute to liver cell loss in this animal model.
authors.affiliation: Department of Histopathology, Queen's Medical Centre, University Hospital, Nottingham, United Kingdom. abed.zaitoun@qmc.nhs.uk
authors.forename: Abed Almuti
authors.initials: AA
authors.lastname: Zaitoun
authors.forename: Gustav
authors.initials: G
authors.lastname: Apelqvist
authors.forename: Hanan
authors.initials: H
authors.lastname: Al-Mardini
authors.forename: Trevor
authors.initials: T
authors.lastname: Gray
authors.forename: Finn
authors.initials: F
authors.lastname: Bengtsson
authors.forename: Christopher Oswold
authors.initials: CO
authors.lastname: Record
mesh_terms: D000818:Animals; D017209:Apoptosis; D001284:Atrophy; D008099:Liver / Q000098:blood supply / Q000473:pathology*; D008297:Male; D008833:Microcirculation; D008938:Mitosis; D011167:Portacaval Shunt, Surgical / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow; D013739:Testosterone / Q000097:blood
chemical_list: D013739:Testosterone