The establishment of a HeLa cell demonstrating rapid mitogen-activated protein kinase phosphorylation in response to 1alpha,25-dihydroxyvitamin D3 by stable transfection of a chick skeletal muscle cDNA library.
1alpha,25-dihydroxyvitamin D3 [1,25-(OH)2D3] phosphorylates the extracellular signal-regulated kinase (ERK), a member of the mitogen-activated protein kinase (MAPK) family, within 30 sec in primary cultured chick skeletal muscle cells. MAPK of HeLa cell lines, which had been stably transfected with a cDNA library derived from mRNA of chick skeletal muscle cells, was also rapidly phosphorylated by 1,25-(OH)2D3. These cell lines have the potential to be a good tool for further investigation of rapid non-genomic mechanism activated by 1,25-(OH)2D3.
Yamauchi
Jun
J
Division of Applied Food Research, National Institute of Health and Nutrition, Tokyo, Japan. junyama@nih.go.jp
D000818:Animals; D002645:Chickens; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D018167:Receptors, Calcitriol / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology
D018167:Receptors, Calcitriol; C118756:dihydroxy-vitamin D3; D014807:Vitamin D; D020928:Mitogen-Activated Protein Kinases