Evaluation of the INOVA diagnostics enzyme-linked immunosorbent assay kits for measuring serum immunoglobulin G (IgG) and IgA to deamidated gliadin peptides.
New assays for antibodies to deamidated gliadin peptides (DGP) expressing celiac disease-specific epitopes were evaluated using 154 sera previously tested for endomysial immunoglobulin A (IgA) (EMA), transglutaminase IgA (TGA), and conventional gliadin antibodies. DGP antibody results showed 97% concordance with EMA and TGA results. Of 56 sera negative for EMA and TGA but positive for conventional gliadin antibodies, 54 (96%) were negative for DGP antibodies.
Prince
Harry E
HE
Focus Diagnostics, Cypress, California 90630, USA. hprince@focusdx.com
D015703:Antigens, CD / Q000378:metabolism; D002446:Celiac Disease / Q000175:diagnosis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005903:Gliadin / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D010455:Peptides / Q000276:immunology*; D011933:Reagent Kits, Diagnostic
D015703:Antigens, CD; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D010455:Peptides; D011933:Reagent Kits, Diagnostic; D005903:Gliadin