title: Genetic complexity and gametocyte production of Plasmodium falciparum in Fulani and Mossi communities in Burkina Faso.
abstract: We have examined Plasmodium falciparum gametocyte prevalence, density and their genetic complexity among children of 2 sympatric ethnic groups (Mossi and Fulani) in villages in Burkina Faso. The 2 groups are known to have distinct differences in their susceptibility and immune responses to malaria. We used RT-PCR and sequence-specific probes to detect and type RNA of the gametocyte-specific protein Pfs48/45. There were no differences in detection rates of asexual forms and gametocytes among the 2 groups, using PCR and RT-PCR, respectively. However, there were significant differences in densities of asexual forms and gametocytes, which were both higher among Mossi than Fulani. Both asexual forms and gametocyte densities were influenced by age and ethnicity. Multiple-clone infections with more than 1 gametocyte genotype were equally prevalent among Fulani and Mossi. These differences can most probably be attributed to genetic differences in malaria susceptibility in the 2 ethnic groups.
authors.affiliation: Institute of Infection and Immunology Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK.
authors.forename: G M
authors.initials: GM
authors.lastname: Paganotti
authors.forename: C
authors.initials: C
authors.lastname: Palladino
authors.forename: D
authors.initials: D
authors.lastname: Modiano
authors.forename: B S
authors.initials: BS
authors.lastname: Sirima
authors.forename: L
authors.initials: L
authors.lastname: Råberg
authors.forename: A
authors.initials: A
authors.lastname: Diarra
authors.forename: A
authors.initials: A
authors.lastname: Konaté
authors.forename: M
authors.initials: M
authors.lastname: Coluzzi
authors.forename: D
authors.initials: D
authors.lastname: Walliker
authors.forename: H A
authors.initials: HA
authors.lastname: Babiker
mesh_terms: D000367:Age Factors; D000818:Animals; D000852:Anopheles / Q000469:parasitology / Q000502:physiology; D002050:Burkina Faso / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004198:Disease Susceptibility / Q000469:parasitology*; D005006:Ethnicity; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006801:Humans; D007223:Infant; D007299:Insect Bites and Stings / Q000453:epidemiology; D007303:Insect Vectors / Q000469:parasitology / Q000502:physiology; D016778:Malaria, Falciparum / Q000453:epidemiology* / Q000235:genetics / Q000469:parasitology; D008562:Membrane Glycoproteins / Q000235:genetics*; D010961:Plasmodium / Q000145:classification; D010963:Plasmodium falciparum / Q000235:genetics* / Q000502:physiology*; D011156:Population Density; D015800:Protozoan Proteins / Q000235:genetics*
chemical_list: D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; C084847:pfs48-45 protein, Plasmodium falciparum