title: Phylogenetic analysis of the glucose-6-phosphate isomerase gene in Trypanosoma cruzi.
abstract: Trypanosoma cruzi, the agent of Chagas disease, has a basically clonal population structure with rare hybridization events. The species is subdivided into six "Discrete Typing Units" called DTUs I, IIa-e, distributed into two major phylogenetic lineages, T. cruzi I and II (TC I and II). The glucose-6-phosphate isomerase (Gpi) is a specific isoenzymic locus that presents homozygous profiles for DTUs I, IIa-c, and typical heterozygous patterns, for DTUs IId and IIe. The gene was sequenced in 12 T. cruzi stocks and in three stocks pertaining to related species. The phylogenetic relationships observed confirm that the DTUs I, IIa-c do constitute monophyletic groups. Nevertheless, the phylogenetic hierarchy of the DTUs is not clearly resolved with the GPI gene. The hybrid status of DTUs IId and IIe was clearly supported. Sequence analysis revealed that the allele 4 present in both DTUs IIa and IIc, previously considered as unique, displayed in fact two distinct sequences, specific for each DTU. The level of recombination between alleles has been investigated.
authors.affiliation: Institut de Recherche pour le Développement (IRD), Génétique des Maladies Infectieuses, Unité de Recherche IRD no 165/Unité Mixte de Recherche CNRS (Centre National de la Recherche Scientifique, 34394 Montpellier cedex 5, France. broutin@mpl.ird.fr
authors.forename: Hélène
authors.initials: H
authors.lastname: Broutin
authors.forename: Frédérique
authors.initials: F
authors.lastname: Tarrieu
authors.forename: Michel
authors.initials: M
authors.lastname: Tibayrenc
authors.forename: Bruno
authors.initials: B
authors.lastname: Oury
authors.forename: Christian
authors.initials: C
authors.lastname: Barnabé
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D014355:Chagas Disease / Q000469:parasitology; D005838:Genotype; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D006801:Humans; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D016415:Sequence Alignment; D014349:Trypanosoma cruzi / Q000145:classification / Q000201:enzymology* / Q000235:genetics*
chemical_list: D005956:Glucose-6-Phosphate Isomerase