title: Unique NS5b hepatitis C virus gene sequence consensus database is essential for standardization of genotype determinations in multicenter epidemiological studies.
abstract: A multicenter study of NS5b hepatitis C virus (HCV) genotype determination involving 12 laboratories demonstrates that any laboratory with expertise in sequencing techniques would be able to provide a reliable HCV genotype for clinical and epidemiological purposes as long as they are provided a consensus reference sequence database.
authors.affiliation: Institut National de la Transfusion Sanguine, 6 rue Alexandre-Cabanel, 75015 Paris, France.
authors.forename: Syria
authors.initials: S
authors.lastname: Laperche
authors.forename: Karine
authors.initials: K
authors.lastname: Saune
authors.forename: Paul
authors.initials: P
authors.lastname: Dény
authors.forename: Gilles
authors.initials: G
authors.lastname: Duverlie
authors.forename: Sophie
authors.initials: S
authors.lastname: Alain
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Chaix
authors.forename: Catherine
authors.initials: C
authors.lastname: Gaudy
authors.forename: Françoise
authors.initials: F
authors.lastname: Lunel
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Pawlotsky
authors.forename: Christopher
authors.initials: C
authors.lastname: Payan
authors.forename: Bruno
authors.initials: B
authors.lastname: Pozzetto
authors.forename: Catherine
authors.initials: C
authors.lastname: Tamalet
authors.forename: Vincent
authors.initials: V
authors.lastname: Thibault
authors.forename: Sophie
authors.initials: S
authors.lastname: Vallet
authors.forename: Françoise
authors.initials: F
authors.lastname: Bouchardeau
authors.forename: Jacques
authors.initials: J
authors.lastname: Izopet
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Lefrère
mesh_terms: D016384:Consensus Sequence; D030541:Databases, Genetic; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics; D006526:Hepatitis C / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D007753:Laboratories / Q000592:standards*; D016133:Polymerase Chain Reaction; D012015:Reference Standards; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase
chemical_list: D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase