Freezing induces biased results in the molecular detection of Flavobacterium columnare.
Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR. The detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish.
Suomalainen
Lotta-Riina
LR
Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35, FIN-40014 University of Jyväskylä, Finland.
Reunanen
Hilkka
H
Ijäs
Ritva
R
Valtonen
E Tellervo
ET
Tiirola
Marja
M
D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005188:False Negative Reactions; D005417:Flavobacterium / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D005615:Freezing; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial