title: Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: thermodynamics data correlated with X-ray structures.
abstract: The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose and D-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with L-galactose and Met-beta-D-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.
authors.affiliation: CERMAV-CNRS (affiliated with Université Joseph Fourier), 601 rue de la Chimie, Grenoble BP53, F-38041 Grenoble cedex 09, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Sabin
authors.forename: Edward P
authors.initials: EP
authors.lastname: Mitchell
authors.forename: Martina
authors.initials: M
authors.lastname: Pokorná
authors.forename: Catherine
authors.initials: C
authors.lastname: Gautier
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Utille
authors.forename: Michaela
authors.initials: M
authors.lastname: Wimmerová
authors.forename: Anne
authors.initials: A
authors.lastname: Imberty
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites / Q000502:physiology; D018441:Biofilms / Q000254:growth & development; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D009005:Monosaccharides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000502:physiology; D013816:Thermodynamics
chemical_list: D018829:Adhesins, Bacterial; D037102:Lectins; D009005:Monosaccharides; C038692:adhesin, Pseudomonas