Immunization of rhesus macaques with a polyvalent DNA prime/protein boost human immunodeficiency virus type 1 vaccine elicits protective antibody response against simian human immunodeficiency virus of R5 phenotype.
The immunogenicity of a poylvalent HIV-1 vaccine comprised of Env antigens from primary R5 isolates was evaluated in rhesus macaques. DNA vaccines encoding four Env antigens from multiple HIV-1 subtypes and HIV-1 Gag antigen from a single subtype elicited a persistent level of binding antibodies to gp120 from multiple HIV-1 isolates that were markedly enhanced following boosting with homologous gp120 proteins in QS-21 adjuvant irrespective of the route of DNA immunization. These sera neutralized homologous and, to a lesser degree, heterologous HIV-1 isolates. Four of the six immunized animals were completely protected following rectal challenge with a SHIV encoding Env from HIV-1(Ba-L), whereas the virus load was reduced in the remaining animals compared to naïve controls. Hence priming with DNA encoding Env antigens from multiple HIV-1 clades followed by boosting with homologous Env proteins elicits anti-HIV-1 immune responses capable of protecting macaques against mucosal transmission of R5 tropic SHIV isolate.
Pal
Ranajit
R
Department of Cell Biology, Advanced BioScience Laboratories, Kensington, MD 20895, USA. Ranajit.Pal@ablinc.com
Kalyanaraman
V S
VS
Nair
B C
BC
Whitney
Stephen
S
Keen
Timothy
T
Hocker
Lindsey
L
Hudacik
Lauren
L
Rose
Nicolas
N
Mboudjeka
Innocent
I
Shen
Siyuan
S
Wu-Chou
Te-Hui
TH
Montefiori
David
D
Mascola
John
J
Markham
Philip
P
Lu
Shan
S
D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D015683:Gene Products, gag; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D007115:Immunization Schedule; D007117:Immunization, Secondary; D008253:Macaca mulatta; D009500:Neutralization Tests; D010641:Phenotype; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control; D015302:Simian Immunodeficiency Virus / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000235:genetics
D016915:AIDS Vaccines; D000914:Antibodies, Viral; D015683:Gene Products, gag; D015699:HIV Envelope Protein gp120; D019444:Vaccines, DNA; D022223:Vaccines, Subunit