title: Dual effect of flurbiprofen on cell proliferation and agonist-induced Ca(2+) movement in human osteosarcoma cells.
abstract: In human MG63 osteosarcoma cells, the effect of flurbiprofen on intracellular Ca(2+) concentrations ([Ca(2+)](i)) and proliferation was explored. The proliferation was enhanced by 20-120 microM flurbiprofen, and was decreased by 140-200 microM flurbiprofen. The effect of flurbiprofen on the increases in cytosolic free Ca(2+) levels ([Ca(2+)](i)) induced by ATP, bradykinin, histamine and thapsigargin (an inhibitor of the endoplasmic reticulum Ca(2+) ATPase), was examined. In cell preincubated with 20 or 80 microM flurbiprofen, the [Ca(2+)](i) increases induced by all agonists were attenuated. In the presence of 20 microM flurbiprofen, the decreased [Ca(2+)](i) responses with the agonists were attributed to a defective Ca(2+) influx because this decrease was unobserved in agonists-induced [Ca(2+)](i) increases in the absence of extracellular Ca(2+). In the presence of 80 microM flurbiprofen, both the Ca(2+) influx component and the Ca(2+) releasing (from organelles) component were defective. These results suggest that flurbiprofen could alter proliferation and inhibit [Ca(2+)](i) increases.
authors.affiliation: Department of Dentistry, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan 813.
authors.forename: Li-Ling
authors.initials: LL
authors.lastname: Tseng
authors.forename: He-Hsiung
authors.initials: HH
authors.lastname: Cheng
authors.forename: Chun-Jen
authors.initials: CJ
authors.lastname: Huang
authors.forename: Shiuh-Inn
authors.initials: SI
authors.lastname: Liu
authors.forename: Chun-Chi
authors.initials: CC
authors.lastname: Kuo
authors.forename: Wei-Chuan
authors.initials: WC
authors.lastname: Chen
authors.forename: Jong-Khing
authors.initials: JK
authors.lastname: Huang
authors.forename: Shu-Shong
authors.initials: SS
authors.lastname: Hsu
authors.forename: Hong-Tai
authors.initials: HT
authors.lastname: Chang
authors.forename: Cheng-Hsing
authors.initials: CH
authors.lastname: Kao
authors.forename: Chin-Man
authors.initials: CM
authors.lastname: Ho
authors.forename: Chung-Ren
authors.initials: CR
authors.lastname: Jan
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001920:Bradykinin / Q000494:pharmacology; D002118:Calcium / Q000819:agonists / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005480:Flurbiprofen / Q000494:pharmacology*; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D012516:Osteosarcoma; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005480:Flurbiprofen; D019284:Thapsigargin; D006632:Histamine; D000255:Adenosine Triphosphate; D001920:Bradykinin; D002118:Calcium