title: MORT1/FADD is involved in liver regeneration.
abstract: OBJECTIVE
  To explore the role of the adaptor molecule in liver regeneration after partial hepatectomy (PH).
  
  
  METHODS
  We used transgenic mice expressing an N-terminal truncated form of MORT1/FADD under the control of the albumin promoter. As previously shown, this transgenic protein abrogated CD95- and CD120a-mediated apoptosis in the liver. Cyclin A expression was detected using Western blotting. ELISA and RT-PCR were used to detect IL-6 and IL-6 mRNA, respectively. DNA synthesis in liver tissue was measured by BrdU staining.
  
  
  RESULTS
  Resection of 70% of the liver was followed by a reduced early regenerative response in the transgenic group at 36 h. Accordingly, 36 h after hepatectomy, cyclin A expression was only detectable in wild-type animals. Consequently, the onset of liver mass restoration was retarded as measured by MRI volumetry and mortality was significantly higher in the transgenic group.
  
  
  CONCLUSIONS
  Our data demonstrate for the first time an involvement of the death receptor molecule MORT1/FADD in liver regeneration, beyond its well described role as part of the intracellular death signaling pathway.
authors.affiliation: Department of Medicine University of Mainz, Langenbeckstr. 1, Mainz 55101, Germany. schuchm@mail.uni-mainz.de
authors.forename: Marcus
authors.initials: M
authors.lastname: Schuchmann
authors.forename: Felix
authors.initials: F
authors.lastname: Ruckert
authors.forename: Jose F
authors.initials: JF
authors.lastname: Garcia-Lazaro
authors.forename: Andrea
authors.initials: A
authors.lastname: Karg
authors.forename: Jurgen
authors.initials: J
authors.lastname: Burg
authors.forename: Natalia
authors.initials: N
authors.lastname: Knorr
authors.forename: Jurgen
authors.initials: J
authors.lastname: Siebler
authors.forename: Eugene E
authors.initials: EE
authors.lastname: Varfolomeev
authors.forename: David
authors.initials: D
authors.lastname: Wallach
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schreiber
authors.forename: Ansgar W
authors.initials: AW
authors.lastname: Lohse
authors.forename: Peter R
authors.initials: PR
authors.lastname: Galle
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000502:physiology*; D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D019925:Cyclin A / Q000378:metabolism; D053200:Fas-Associated Death Domain Protein; D006498:Hepatectomy; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000235:genetics / Q000502:physiology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D019925:Cyclin A; C505534:Fadd protein, mouse; D053200:Fas-Associated Death Domain Protein; D015850:Interleukin-6; D010446:Peptide Fragments