Detection of rat parvovirus type 1 and rat minute virus type 1 by polymerase chain reaction.
Two newly recognized parvovirus species, rat parvovirus 1 (RPV-1) and rat minute virus 1 (RMV-1), were recently identified in naturally infected rats. In this study, two polymerase chain reaction (PCR) assays were developed to specifically detect RPV-1 and RMV-1. The RPV-1 PCR assay amplified the expected 487-bp deoxyribonucleic acid (DNA) fragment only in the presence of RPV-1 DNA; the RMV-1 PCR assay amplified the expected 843-bp product only from RMV-1 DNA, not from other rodent parvoviruses. The RPV-1 and the RMV-1 PCR assays detected approximately 18 and 70 copies of DNA template, respectively. These two PCR assays were shown to be sensitive, specific and rapid methods for detecting RPV-1 and RMV-1 infections in rats. These assays may also be valuable for evaluation of biological specimens for parvovirus contamination.
Wan
C-H
CH
Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO 65211, USA. chwan@ntu.edu.tw
Bauer
B A
BA
Pintel
D J
DJ
Riley
L K
LK
D000818:Animals; D000831:Animals, Newborn; D001681:Biological Assay; D006224:Cricetinae; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D051379:Mice; D008915:Minute Virus of Mice / Q000235:genetics / Q000302:isolation & purification; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017992:Parvovirus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D051381:Rats; D012376:Rodent Diseases / Q000175:diagnosis* / Q000821:virology; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004279:DNA, Viral