title: Hemorheological abnormalities in lipoprotein lipase deficient mice with severe hypertriglyceridemia.
abstract: Severe hypertriglyceridemia (HTG) is a metabolic disturbance often seen in clinical practice. It is known to induce life-threatening acute pancreatitis, but its role in atherogenesis remains elusive. Hemorheological abnormality was thought to play an important role in pathogenesis of both pancreatitis and atherosclerosis. However, hemorheology in severe HTG was not well investigated. Recently, we established a severe HTG mouse model deficient in lipoprotein lipase (LPL) in which severe HTG was observed to cause a significant increase in plasma viscosity. Disturbances of erythrocytes were also documented, including decreased deformability, electrophoresis rate, and membrane fluidity, and increased osmotic fragility. Scanning electron microscopy demonstrated that most erythrocytes of LPL deficient mice deformed with protrusions, irregular appearances or indistinct concaves. Analysis of erythrocyte membrane lipids showed decreased cholesterol (Ch) and phospholipid (PL) contents but unaltered Ch/PL ratio. The changes of membrane lipids may be partially responsible for the hemorheological and morphologic abnormalities of erythrocytes. This study indicated that severe HTG could lead to significant impairment of hemorheology and this model may be useful in delineating the role of severe HTG in the pathogenesis of hyperlipidemic pancreatitis and atherosclerosis.
authors.affiliation: Institute of Cardiovascular Sciences, Health Science Center, Peking University, Beijing 100083, China.
authors.forename: Tieqiang
authors.initials: T
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Xunde
authors.initials: X
authors.lastname: Xian
authors.forename: Colin J D
authors.initials: CJ
authors.lastname: Ross
authors.forename: Michael R
authors.initials: MR
authors.lastname: Hayden
authors.forename: Zongyao
authors.initials: Z
authors.lastname: Wen
authors.forename: George
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D001809:Blood Viscosity; D004910:Erythrocyte Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000473:pathology; D018056:Hemorheology; D015228:Hypertriglyceridemia / Q000097:blood*; D008071:Lipoprotein Lipase / Q000172:deficiency*; D008563:Membrane Lipids / Q000097:blood; D051379:Mice; D009996:Osmotic Fragility
chemical_list: D008563:Membrane Lipids; D008071:Lipoprotein Lipase