title: Isolation of Rickettsia felis in the mosquito cell line C6/36.
abstract: We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene).
authors.affiliation: Faculty of Veterinary Medicine, University of Sao Paulo, São Paulo, SP 05508-270, Brazil.
authors.forename: Maurício C
authors.initials: MC
authors.lastname: Horta
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Labruna
authors.forename: Edison L
authors.initials: EL
authors.lastname: Durigon
authors.forename: Teresinha T S
authors.initials: TT
authors.lastname: Schumaker
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001431:Bacteriological Techniques; D002460:Cell Line; D005798:Genes, Bacterial; D041161:Rickettsia felis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D001425:Bacterial Outer Membrane Proteins