Transcranial imaging of audiogenic epileptic foci in the cortex of DBA/2J mice.
Exposure to intense sound stimuli induces audiogenic seizures in DBA/2J mice. We investigated cortical activities during sound stimulation using flavoprotein fluorescence imaging. Most DBA/2J mice had seizures during intense sound stimulation, with more than half surviving after seizures. Surviving mice were anesthetized with urethane (1.6 g/kg, intraperitoneal), and the skull was exposed and then covered with clear resin. More than 3 days after surgery, the mice were lightly anesthetized with urethane (0.8 g/kg) and cortical activities during intense sound stimulation were visualized. Focal responses appeared near the somatosensory cortex together with spike activities localized in the response area. These findings indicate that epileptic foci of audiogenic seizure are formed in the cortex of DBA/2J mice.
Takao
Tetsuro
T
Department of Neurophysiology, Brain Research Institute, Niigata University, Niigata, Japan.
Murakami
Hiroatsu
H
Fukuda
Masafumi
M
Kawaguchi
Tadashi
T
Kakita
Akiyoshi
A
Takahashi
Hitoshi
H
Kudoh
Masaharu
M
Tanaka
Ryuichi
R
Shibuki
Katsuei
K
D000161:Acoustic Stimulation / Q000009:adverse effects*; D000818:Animals; D001931:Brain Mapping; D003952:Diagnostic Imaging / Q000379:methods*; D020195:Epilepsy, Reflex / Q000209:etiology* / Q000503:physiopathology; D005072:Evoked Potentials, Auditory / Q000502:physiology*; D005420:Flavoproteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013003:Somatosensory Cortex / Q000503:physiopathology*; D013045:Species Specificity; D013997:Time Factors
D005420:Flavoproteins