On the influence of oxygen and cell concentration in an SFPR whole cell biocatalytic Baeyer-Villiger oxidation process.
Efficient whole cell biotransformations, in particular microbial whole cell Baeyer-Villiger oxidation with molecular oxygen, demand comprehension and optimization of the process details involved. Optimal provision of oxygen and control of bioprocess parameters are pivotal for their success. The interrelation of cell density and oxygen supply in an in situ substrate feeding and product removal (SFPR) whole cell Baeyer-Villiger oxidation process was investigated in detail. Both parameters were optimized with respect to practical considerations. The outcome of this study supports a schematic process model, allows estimation of optimum process conditions and exploration of its limits.
Hilker
Iris
I
Department of Biochemistry, Fluka Group, Industriestrasse 25, CH-9470 Buchs, Switzerland.
Baldwin
Chris
C
Alphand
Véronique
V
Furstoss
Roland
R
Woodley
John
J
Wohlgemuth
Roland
R
D019149:Bioreactors / Q000382:microbiology*; D001711:Biotransformation; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000378:metabolism; D002455:Cell Division; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D007659:Ketones / Q000378:metabolism*; D007700:Kinetics; D007783:Lactones / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D010313:Partial Pressure
D019086:Bridged Bicyclo Compounds, Heterocyclic; D007659:Ketones; D007783:Lactones; D010105:Oxygenases; C019961:cyclohexanone oxygenase; D010100:Oxygen