Differential gene expression in central nervous system tissues of sheep with natural scrapie.
The expression of nine genes was analyzed by real-time RT-PCR in the central nervous system in order to investigate the molecular pathogenesis of natural scrapie. An up-regulation of genes related to glial activation (GFAP) and apoptosis (CASP3) was detected in obex and cerebrum, indicating a reactive glia. Another glial activation-related gene (CTSS) was slightly up-regulated in obex, whereas constitutive expression was detected for SOD1, YWHAZ, PRNP, and the apoptosis-related genes BCL2, MCL1, and BAX. This differential gene expression might reflect a spatial-temporal and tissue-specific molecular pathogenesis of scrapie.
Garcia-Crespo
David
D
Department of Animal Health, Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER); Berreaga, 1. 48160 Derio, Bizkaia, Spain.
Juste
Ramón A
RA
Hurtado
Ana
A
D000682:Amyloid / Q000235:genetics / Q000378:metabolism; D000818:Animals; D053148:Caspase 3; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism* / Q000473:pathology; D051525:Fibroblast Growth Factor 9 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D011328:Prions; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012608:Scrapie / Q000378:metabolism*; D012756:Sheep; D018709:Statistics, Nonparametric; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1
D000682:Amyloid; D051525:Fibroblast Growth Factor 9; D005904:Glial Fibrillary Acidic Protein; D018832:Molecular Chaperones; D011328:Prions; D011498:Protein Precursors; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D053148:Caspase 3; D020169:Caspases