Measurement of respiration rates of Methylobacterium extorquens AM1 cultures by use of a phosphorescence-based sensor.
Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This method was tested with the facultative methylotroph Methylobacterium extorquens AM1 to demonstrate subtle mutant phenotypes.
Strovas
Tim J
TJ
Department of Bioengineering, Microscale Life Sciences Center, University of Washington, Seattle, Washington 98195-2180, USA.
Dragavon
Joe M
JM
Hankins
Tyler J
TJ
Callis
James B
JB
Burgess
Lloyd W
LW
Lidstrom
Mary E
ME
D004566:Electrodes; D042967:Electron Transport Complex I / Q000235:genetics; D005798:Genes, Bacterial; D007700:Kinetics; D008163:Luminescent Measurements; D020601:Methylobacterium extorquens / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010101:Oxygen Consumption; D010641:Phenotype; D011166:Porphyrins
D011166:Porphyrins; D042967:Electron Transport Complex I