Does the rate of rewarming from profound hypothermic arrest influence the outcome in a swine model of lethal hemorrhage?
BACKGROUND
Rapid induction of profound hypothermic arrest (suspended animation) can provide valuable time for the repair of complex injuries and improve survival. The optimal rate for re-warming from a state of profound hypothermia is unknown. This experiment was designed to test the impact of different warming rates on outcome in a swine model of lethal hemorrhage from complex vascular injuries.


METHODS
Uncontrolled lethal hemorrhage was induced in 40 swine (80-120 lbs) by creating an iliac artery and vein injury, followed 30 minutes later (simulating transport time) by laceration of the descending thoracic aorta. Through a thoracotomy approach, a catheter was placed in the aorta and hyperkalemic organ preservation solution was infused on cardiopulmonary bypass to rapidly (2 degrees C/min) induce profound (10 degrees C) hypothermia. Vascular injuries were repaired during 60 minutes of hypothermic arrest. The 4 groups (n = 10/group) included normothermic controls (NC) where core temperature was maintained between 36 to 37 degrees C, and re-warming from profound hypothermia at rates of: 0.25 degrees C/min (slow), 0.5 degrees C/min (medium), or 1 degrees C/min (fast). Hyperkalemia was reversed during the hypothermic arrest period, and blood was infused for resuscitation during re-warming. After discontinuation of cardiopulmonary bypass, the animals were recovered and monitored for 6 weeks for neurologic deficits, cognitive function (learning new skills), and organ dysfunction. Detailed examination of brains was performed at 6 weeks.


RESULTS
All the normothermic animals died, whereas survival rates for slow, medium and fast re-warming from hypothermic arrest were 50, 90, and 30%, respectively (p < 0.05 slow and medium warming versus normothermic control, p < 0.05 medium versus fast re-warming). All the surviving animals were neurologically intact, displayed normal learning capacity, and had no long-term organ dysfunction.


CONCLUSIONS
Rapid induction of hypothermic arrest maintains viability of brain during repair of lethal vascular injuries. Long-term survival is influenced by the rate of reversal of hypothermia.
Alam
Hasan B
HB
Trauma Research and Readiness Institute for Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. hbalam@partners.org
Rhee
Peter
P
Honma
Kaneatsu
K
Chen
Huazhen
H
Ayuste
Eduardo C
EC
Lin
Tom
T
Toruno
Kevin
K
Mehrani
Tina
T
Engel
Caroline
C
Chen
Zheng
Z
D000818:Animals; D015415:Biomarkers / Q000097:blood; D050792:Circulatory Arrest, Deep Hypothermia Induced; D003072:Cognition Disorders / Q000097:blood / Q000209:etiology; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D017694:Rewarming / Q000379:methods* / Q000523:psychology; D012771:Shock, Hemorrhagic / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D017695:Soft Tissue Injuries / Q000097:blood / Q000150:complications / Q000601:surgery*; D013552:Swine; D013997:Time Factors; D016896:Treatment Outcome
D015415:Biomarkers; D016207:Cytokines