title: Optical detection of DNA conformational polymorphism on single-walled carbon nanotubes.
abstract: The transition of DNA secondary structure from an analogous B to Z conformation modulates the dielectric environment of the single-walled carbon nanotube (SWNT) around which it is adsorbed. The SWNT band-gap fluorescence undergoes a red shift when an encapsulating 30-nucleotide oligomer is exposed to counter ions that screen the charged backbone. The transition is thermodynamically identical for DNA on and off the nanotube, except that the propagation length of the former is shorter by five-sixths. The magnitude of the energy shift is described by using an effective medium model and the DNA geometry on the nanotube sidewall. We demonstrate the detection of the B-Z change in whole blood, tissue, and from within living mammalian cells.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Heller
authors.forename: Esther S
authors.initials: ES
authors.lastname: Jeng
authors.forename: Tsun-Kwan
authors.initials: TK
authors.lastname: Yeung
authors.forename: Brittany M
authors.initials: BM
authors.lastname: Martinez
authors.forename: Anthonie E
authors.initials: AE
authors.lastname: Moll
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Gastala
authors.forename: Michael S
authors.initials: MS
authors.lastname: Strano
mesh_terms: D016475:3T3 Cells; D000042:Absorption; D000327:Adsorption; D000818:Animals; D002413:Cations, Divalent / Q000737:chemistry; D002645:Chickens; D002942:Circular Dichroism; D004247:DNA / Q000097:blood / Q000737:chemistry*; D043542:DNA, Z-Form / Q000097:blood / Q000737:chemistry*; D005453:Fluorescence; D008433:Mathematics; D008628:Mercury / Q000032:analysis; D051379:Mice; D008958:Models, Molecular; D018482:Muscle, Skeletal / Q000737:chemistry; D037742:Nanotubes, Carbon; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D002413:Cations, Divalent; D043542:DNA, Z-Form; D037742:Nanotubes, Carbon; D009838:Oligodeoxyribonucleotides; D004247:DNA; D008628:Mercury