title: Human KLF17 is a new member of the Sp/KLF family of transcription factors.
abstract: The Sp/KLF transcription factors perform a variety of biological functions, but are related in that they bind GC-box and CACCC-box sequences in DNA via a highly conserved DNA-binding domain. A database homology search, using the zinc finger DNA-binding domain characteristic of the family, has identified human KLF17 as a new family member that is most closely related to KLFs 1-8 and 12. KLF17 appears to be the human orthologue of the previously reported mouse gene, zinc finger protein 393 (Zfp393), although it has diverged significantly. The DNA-binding domain is the most conserved region, suggesting that both the murine and the human forms recognize the same binding sites in DNA and may retain similar functions. We show that human KLF17 can bind G/C-rich sites via its zinc fingers and is able to activate transcription from CACCC-box elements. This is the first report of the DNA-binding characteristics and transactivation activity of human KLF17, which, together with the homology it displays to other KLF proteins, put it in the Sp/KLF family.
authors.affiliation: School of Molecular and Microbial Biosciences, G08, University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Jane
authors.initials: J
authors.lastname: van Vliet
authors.forename: Linda A
authors.initials: LA
authors.lastname: Crofts
authors.forename: Kate G R
authors.initials: KG
authors.lastname: Quinlan
authors.forename: Robert
authors.initials: R
authors.lastname: Czolij
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Perkins
authors.forename: Merlin
authors.initials: M
authors.lastname: Crossley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D019295:Computational Biology; D017124:Conserved Sequence; D016208:Databases, Factual; D024202:Electrophoretic Mobility Shift Assay; D020224:Expressed Sequence Tags; D015870:Gene Expression; D006801:Humans; D007313:Insecta / Q000166:cytology; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D016329:Sp1 Transcription Factor / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015032:Zinc / Q000737:chemistry; D016335:Zinc Fingers / Q000235:genetics
chemical_list: C510638:KLF17 protein, human; D051741:Kruppel-Like Transcription Factors; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D015032:Zinc