Synthesis of DnaK and GroEL in Escherichia coli cells exposed to different magnetic field signals.
The effects of extremely low frequency magnetic field (ELF-MF)(1 mT, 50 Hz) on the heat shock protein (HSP) synthesis in Escherichia coli were investigated. Two magnetic field signals were studied: sinusoidal (SMF) and pulsed square wave (PMF). It was found that bacteria exposed to SMF showed a significantly higher level of DnaK and GroEL proteins as compared to sham-exposed bacteria as revealed by Western blot, whereas a lower level was observed after PMF exposure. Similar results were obtained when bacterial cells were exposed to heat shock (HS) after ELF-MF exposure: again SMF and PMF resulted in an increase and in a reduction of HSP amount in comparison with sham control, respectively. In conclusion, the MF influences the synthesis of HSPs in E. coli in a way that critically depends on the signal characteristics.
Del Re
Brunella
B
Department of Evolutionary Experimental Biology, University of Bologna, via Selmi 3, 40126 Bologna, Italy.
Bersani
Ferdinando
F
Mesirca
Pietro
P
Giorgi
Gianfranco
G
D018834:Chaperonin 60 / Q000096:biosynthesis*; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D029968:Escherichia coli Proteins / Q000096:biosynthesis*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*
D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; C063842:dnaK protein, E coli