Lack of correlation between the vitamin D receptor Fokl start codon polymorphism and bone mineral density in patients with Crohn's disease.
BACKGROUND
We have examined the association of bone mineral density of patients with inflammatory bowel disease with a polymorphism in the gene encoding the vitamin D receptor. The thymine/cytosine (T/C) polymorphism in the first of two start codons can be defined by a restriction fragment length polymorphism using the restriction endonuclease FokI. Vitamin D receptor alleles containing the polymorphism have been denoted by f and alleles lacking the site by F.


METHODS
We report on an association analysis of a basic population of 244 caucasian patients with Crohn's disease. We have genotyped the FokI polymorphism of the VDR in these patients and associated the genotype with the bone mineral density of the lumbar spine and the femoral neck.


RESULTS
In the cohort 42% of the patients were scored FF homozygous, 43.7% Ff heterozygous, and 14.3% ff homozygous. 14.4% of the FF patients, 18.8% of the Ff patients, and 9.7% of the ff patients had osteoporosis of the lumbar spine and 21.25% of the FF patients, 25.3% of the Ff patients, and 18.5% of the ff patients had osteoporosis of the femoral neck. In this cohort no association between the genotype and the bone mineral density in the group as a whole nor when separated according to sex or age was found.


CONCLUSIONS
In summary in our cohort no association of the FokI polymorphism and the BMD of the lumbar spine and femoral neck in patients with inflammatory bowel disease was found.
Bregenzer
N
N
Department of Internal Medicine I, University of Regensburg, Regensburg, Germany. nicole.bregenzer@klinik.uni-regensburg.de
Schäffler
A
A
Gelbmann
C M
CM
Steinkamp
M
M
Reinshagen
M
M
Schölmerich
J
J
Andus
T
T
D015519:Bone Density; D002574:Cervical Vertebrae / Q000503:physiopathology; D018387:Codon, Initiator; D003424:Crohn Disease / Q000235:genetics* / Q000503:physiopathology; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005260:Female; D005838:Genotype; D006801:Humans; D008159:Lumbar Vertebrae / Q000503:physiopathology; D008297:Male; D020641:Polymorphism, Single Nucleotide; D018167:Receptors, Calcitriol / Q000235:genetics*
D018387:Codon, Initiator; D018167:Receptors, Calcitriol; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific