title: Protein insertion and patterning of PEG bearing langmuir monolayers.
abstract: Protein adsorption to multicomponent lipid monolayers is presented as a means of inducing protein-specific binding pockets or imprints in membranes. Adsorption of the acidic protein ferritin to Langmuir monolayers of cationic dioctadecyldimethylammonium bromide (DOMA), nonionic methyl stearate (SME), and poly(ethylene glycol) (PEG) bearing phospholipids is investigated as a model system. The number, size, and distribution of protein binding pockets (domains of SME and DOMA in a PEG matrix) are defined by controlling the molar ratios, miscibility, and lateral mobility of the lipids. Protein patterning of binary SME:DOMA monolayers is limited by protein-protein interactions that hinder desorption to regenerate the imprint site. The incorporation of PEG bearing phospholipids as a third lipid component provides a successful approach to prevent protein surface aggregation during imprinting. Atomic force microscopy reveals a user-defined distribution of protein molecules where protein-protein interactions on the monolayer are eliminated, thus facilitating protein desorption and regeneration of the protein binding pockets.
authors.affiliation: Department of Biological, Utah State University,4105 Old Main Hill, Logan, Utah, 84322-4105, USA.
authors.forename: Harshil
authors.initials: H
authors.lastname: Dhruv
authors.forename: Revathi
authors.initials: R
authors.lastname: Pepalla
authors.forename: Mundeta
authors.initials: M
authors.lastname: Taveras
authors.forename: David W
authors.initials: DW
authors.lastname: Britt
mesh_terms: D000327:Adsorption; D001665:Binding Sites; D008563:Membrane Lipids / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D011092:Polyethylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D008563:Membrane Lipids; D011506:Proteins; D013501:Surface-Active Agents; D011092:Polyethylene Glycols