title: Measurement of respiration rates of Methylobacterium extorquens AM1 cultures by use of a phosphorescence-based sensor.
abstract: Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This method was tested with the facultative methylotroph Methylobacterium extorquens AM1 to demonstrate subtle mutant phenotypes.
authors.affiliation: Department of Bioengineering, Microscale Life Sciences Center, University of Washington, Seattle, Washington 98195-2180, USA.
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Strovas
authors.forename: Joe M
authors.initials: JM
authors.lastname: Dragavon
authors.forename: Tyler J
authors.initials: TJ
authors.lastname: Hankins
authors.forename: James B
authors.initials: JB
authors.lastname: Callis
authors.forename: Lloyd W
authors.initials: LW
authors.lastname: Burgess
authors.forename: Mary E
authors.initials: ME
authors.lastname: Lidstrom
mesh_terms: D004566:Electrodes; D042967:Electron Transport Complex I / Q000235:genetics; D005798:Genes, Bacterial; D007700:Kinetics; D008163:Luminescent Measurements; D020601:Methylobacterium extorquens / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010101:Oxygen Consumption; D010641:Phenotype; D011166:Porphyrins
chemical_list: D011166:Porphyrins; D042967:Electron Transport Complex I