title: Distribution of chondroitin sulfate in human endometrium.
abstract: Sulfated glycosaminoglycan (GAG) composition was characterized in the human endometrium during proliferative and secretory phases of the menstrual cycle. Sulfated GAGs were analyzed in endometrium tissue using metachromatic staining, biochemical analysis including electrophoresis before and after specific enzymatic or chemical degradations, and immunostaining with an antibody against chondroitin sulfate (CS). Our results showed that CS was the main sulfated GAG species detected, accompanied by small amounts of heparan sulfate and dermatan sulfate. CS was distributed overall the connective stroma, around arteriole vessels and glands, and there was no important difference in the immunostaining between the proliferative and secretory endometrium phases. Our findings extend previous observations on the GAG composition in the human endometrium providing new information regarding the tissue distribution and location of endometrial CS.
authors.affiliation: Departamento de Histologia e Embriologia, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Cidade Universitária-Ilha do Fundão, 21941-590 Rio de Janeiro, RJ Brazil. nasciutt@ufrj.br
authors.forename: Luiz E
authors.initials: LE
authors.lastname: Nasciutti
authors.forename: Renato
authors.initials: R
authors.lastname: Ferrari
authors.forename: Plínio T
authors.initials: PT
authors.lastname: Berardo
authors.forename: Maísa L S
authors.initials: ML
authors.lastname: Souza
authors.forename: Christina M
authors.initials: CM
authors.lastname: Takiya
authors.forename: Radovan
authors.initials: R
authors.lastname: Borojevic
authors.forename: Maurício S
authors.initials: MS
authors.lastname: Abrão
authors.forename: Luiz-Claudio F
authors.initials: LC
authors.lastname: Silva
mesh_terms: D000328:Adult; D002809:Chondroitin Sulfates / Q000378:metabolism*; D004717:Endometrium / Q000033:anatomy & histology* / Q000378:metabolism*; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008597:Menstrual Cycle / Q000378:metabolism; D008875:Middle Aged; D014018:Tissue Distribution
chemical_list: D006025:Glycosaminoglycans; D002809:Chondroitin Sulfates