Cloning and characterization of a fur homologue from Azospirillum brasilense Sp7.
A homologue of the ferric uptake regulator gene, fur, was identified from a Azospirillum brasilense Sp7 genomic DNA clone. Experiments performed with transcriptional lacZ fusions demonstrated that the A. brasilense fur homologue regulated the expression of two fur regulated Escherichia coli genes: fiu (ferric iron uptake) and fhuF (ferric hydroxamate uptake). A differential regulation by the cognate Fur and the heterologous Fur homologue in response to the iron status of the growth medium was also observed.
Alahari
Anuradha
A
Unité Interactions Plantes-Microorganismes et Santé végétale, CNRS-INRA-Université de Nice-Sophia Antipolis, France.
Tripathi
Anil Kumar
AK
Le Rudulier
Daniel
D
D000595:Amino Acid Sequence; D015806:Azospirillum brasilense / Q000201:enzymology / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism*; D033862:Iron-Binding Proteins; D008969:Molecular Sequence Data; D033902:Periplasmic Binding Proteins; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014169:Transformation, Bacterial
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D033862:Iron-Binding Proteins; D033902:Periplasmic Binding Proteins; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; D007501:Iron