Cationic lipid vesicles as coating precursors in capillary electrochromatography: separation of basic proteins and neutral steroids.
1,2-Dioleyl-3-trymethylammoniumpropane (DOTAP) lipid vesicles were employed as coating precursors to obtain a semipermanent cationic lipid bilayer in silica capillary. The coating procedure was relatively fast and simple. Reliable results for the separation of four basic proteins (alpha-chymotrypsinogen A, ribonuclease A, cytochrome C, lysozyme) were obtained by using an acetate buffer under acidic conditions. The RSDs of the migration times were not higher than 0.5% run-to-run and about 1% day-to-day (3 days), while the RSDs of the peak areas were within 7% day-to-day (3 days). The day-to-day RSD of the EOF mobility of about 1%, confirmed that the DOTAP coating was stable for the separation of basic proteins, under acidic buffers. In addition to basic proteins the DOTAP coating was found suitable under acidic conditions for the repeatable separation of neutral steroids. The potential of DOTAP as a carrier in background electrolyte solution was studied.
Bonoli
Matteo
M
Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Finland.
Varjo
Sami J O
SJ
Wiedmer
Susanne K
SK
Riekkola
Marja-Liisa
ML
D019326:17-alpha-Hydroxyprogesterone / Q000302:isolation & purification; D000450:Aldosterone / Q000302:isolation & purification; D000735:Androstenedione / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000295:instrumentation; D002919:Chymotrypsinogen / Q000302:isolation & purification; D045304:Cytochromes c / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry*; D009113:Muramidase / Q000302:isolation & purification; D011374:Progesterone / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012259:Ribonuclease, Pancreatic / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry; D013256:Steroids / Q000302:isolation & purification*; D013739:Testosterone / Q000302:isolation & purification
D005229:Fatty Acids, Monounsaturated; D008051:Lipid Bilayers; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D013256:Steroids; D013739:Testosterone; D000735:Androstenedione; D000450:Aldosterone; D011374:Progesterone; D019326:17-alpha-Hydroxyprogesterone; D012822:Silicon Dioxide; D045304:Cytochromes c; D002919:Chymotrypsinogen; D012259:Ribonuclease, Pancreatic; D009113:Muramidase; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane