Development of a rapid and simple Agrobacterium tumefaciens-mediated transformation system for the fungal pathogen Heterobasidion annosum.
Heterobasidion annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion annosum TC 32-1 was cocultivated at pH 5.6 and 20 degrees C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.
Samils
Nicklas
N
Department of Forest Mycology & Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Elfstrand
Malin
M
Czederpiltz
Daniel L Lindner
DL
Fahleson
Jan
J
Olson
Ake
A
Dixelius
Christina
C
Stenlid
Jan
J
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000254:growth & development; D001487:Basidiomycota / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic / Q000502:physiology