Incorporation of flow injection analysis or capillary electrophoresis with resonance Rayleigh scattering detection for inorganic ion analysis.
Resonance Rayleigh scattering (RRS) has been explored as a detection (RRSD) technique for capillary electrophoresis (CE) or flow injection analysis (FIA) of inorganic ions. The detection was achieved through a scattering probe of ion-association complex formed from rhodamine B (Rh B) and iodine. The probe scatters strongly at 630 nm when oxidants such as Cr(2)O(7)(2-), MnO(4)(-) and ClO(-) present in a mixed solution of Rh B and iodide. The scattering disappears once iodine is reduced by reductants. Oxidant or reductant species in a sample can thus be detected by positive or negative RRS signal. To verify the RRSD, FIA-RRSD was first constructed and continuous measurement of testing samples containing Cr(2)O(7)(2-), MnO(4)(-) and/or ClO(-) was performed. The detection limits reached a level of decade nM and a linear range was found between peak height and concentration at the range of 0.255-2.04microM for Cr(2)O(7)(2-), 0.158-3.16microM for MnO(4)(-), and 1.18-9.43microM for ClO(-), with linear regression coefficients of all above 0.99. The run-to-run relative standard deviation of peak height was less than 3% (n=6). CE-RRSD was then set up and studied, using a capillary of 75microm i.d.x33cm filled with a running buffer of 50mM citrate and 25mM Tris (pH 3.32) and worked under -12kV at room temperature. The CE eluent was at-line conducted into a stream of rhodamine B and iodine flowing inner a wide tube by plugging the capillary outlet into the wide tube. Different mixtures prepared from Cr(2)O(7)(2-), MnO(4)(-) and ClO(-) were successfully separated and detected by the CE-RRSD.
Qi
Li
L
Laboratory of Analytical Chemistry for Life Science, Department of Chemical Biology, Institute of Chemistry, Chinese Academy of Sciences, P.O. Box 2709, Beijing 100080, PR China.
Han
Zhi-qiang
ZQ
Chen
Yi
Y
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D007455:Iodine / Q000737:chemistry; D017895:Manganese Compounds / Q000032:analysis*; D010087:Oxides / Q000032:analysis*; D010472:Perchlorates / Q000032:analysis*; D011192:Potassium Dichromate / Q000032:analysis*; D012235:Rhodamines / Q000737:chemistry; D013997:Time Factors
D017895:Manganese Compounds; D010087:Oxides; D010472:Perchlorates; D012235:Rhodamines; C048856:permanganic acid; D007455:Iodine; C029773:rhodamine B; D011192:Potassium Dichromate; C494474:perchlorate