title: Structural transition in inactive Balbiani ring chromatin of Chironomus during micrococcus nuclease digestion.
abstract: We have analysed by micrococcus nuclease digestion the chromatin structure of genes in the Balbiani ring (BR) regions of a Chironomus cell line. Gel electrophoresis of the DNA fragments reveals a repeating structure which consists of two repeat sizes, a long repeat seen in the large fragments and a small repeat seen in the small fragments. The two repeats hardly overlap, except in a narrow transition zone which is at a different fragment size in the BR 2.2 and the BR 2.1 gene. The sizes of the large repeats fit the repeat of the underlying DNA sequence. The short repeats are between 170 and 180 bp, and after H1 depletion the short repeat in the BR 2.2 gene is 160 bp. Our most favoured interpretation of these data is that in intact chromatin the nucleosomes in the BR genes are phased with respect to the repeating DNA sequence, whereas micrococcus nuclease digestion leads to loss of a nucleosome-positioning constraint and hence to rearrangement of the nucleosomes. Our results imply a possible artefact of nuclease digestion of chromatin, which has to be taken into account in mapping nucleosome positions.
authors.affiliation: Institut für Zellbiologie, ETH Hönggerberg, CH-8093 Zürich, Switzerland.
authors.forename: R M
authors.initials: RM
authors.lastname: Widmer
authors.forename: M
authors.initials: M
authors.lastname: Lezzi
authors.forename: T
authors.initials: T
authors.lastname: Koller