Hexacoordination of bacteriochlorophyll in photosynthetic antenna LH1.
The ability of chlorophylls to coordinate ligands is of fundamental structural importance for photosynthetic pigment-protein complexes, where in virtually all cases the pigment is thought to be in a pentacoordinated state. In this study, the correlation of the Q(X) transition energy with the coordination state of the central metal in bacteriochlorophyll is applied in investigating the pigment coordination state in bacterial photosynthetic antenna LH1. To facilitate a detailed spectral analysis in the Q(X) region, carotenoid-depleted forms of LH1 are prepared and model LH1 are constructed with non-native carotenoids having blue-shifted absorption. The deconvolution of the Q(X) envelope in LH1 reveals that the band is the sum of two transitions, which peak near 590 and 607 nm, showing that a significant fraction (up to 25%) of hexacoordinated bacteriochlorophyll is present in the complex. The hexacoordination can be seen also in LH1 antennae from other species of purple photosynthetic bacteria. It seems correlated with the LH1 aggregation state and probably is a consequence of the structural flexibility of the assembled complex. The sixth ligand probably originates from the apoprotein and seems not to affect the chromophore core size. These findings show that in light-harvesting complexes a hexacoordinated state of bacteriochlorophyll is not uncommon. Its presence may be relevant to a correct assembly of the antenna and have functional consequences, as it results in a splitting of the pigment S2 excited state (Q(X)), i.e., the carotenoid excitation acceptor state, what might affect intracomplex carotenoid-to-bacteriochlorophyll energy transfer.
Fiedor
Leszek
L
Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Cracow, Poland. lfiedor@mol.uj.edu.pl
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001429:Bacteriochlorophylls / Q000737:chemistry* / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism; D004735:Energy Transfer; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D013057:Spectrum Analysis
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D008024:Ligands; D045342:Light-Harvesting Protein Complexes; C094744:light-harvesting complex 1, Rhodospirillum; D002338:Carotenoids