title: The effects of the side chains of hydrophobic aliphatic amino acid residues in an amphipathic polypeptide on the formation of alpha helix and its association.
abstract: The polypeptide alpha3, which was synthesized by us to produce an amphipathic helix structure, contains the regular three times repeated sequence (LETLAKA)(3), and alpha3 forms a fibrous assembly. To clarify how the side chains of amino acid residues affect the formation of alpha helix, Leu residues, which are located in the hydrophobic surface of an amphipathic helix, were replaced by other hydrophobic aliphatic amino acid residues systematically, and the characters of the resulting polypeptides were studied. According to the circular dichroism (CD) spectra, the Ile-substituted polypeptides formed alpha helix like alpha3. However, their helix formation ability was weaker than that of alpha3 under some conditions. The Val-substituted polypeptides formed alpha helix only under restricted condition. The Ala-substituted polypeptides did not form alpha helix under any condition. Thus, it is clear that the order of the alpha helix formation ability is as follows: Leu >or= Ile > Val > Ala. The formation of alpha helix was confirmed by Fourier Transform Infrared (FTIR) spectra. Through electron microscopic observation, it was clarified that the formation of the alpha helix structure correlates with the formation of a fibrous assembly. The amphipathic alpha helix structure would be stabilized by the formation of the fibrous assembly.
authors.affiliation: Institute for Biomolecular Science, Gakusyuin University, 1-5-1 Mejiro, Tokyo 171-8588, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Takei
authors.forename: Atsuhito
authors.initials: A
authors.lastname: Okonogi
authors.forename: Kumiko
authors.initials: K
authors.lastname: Tateno
authors.forename: Akiko
authors.initials: A
authors.lastname: Kimura
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kojima
authors.forename: Kazumori
authors.initials: K
authors.lastname: Yazaki
authors.forename: Kin-ichiro
authors.initials: K
authors.lastname: Miura
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D002942:Circular Dichroism; D005818:Genetic Engineering / Q000379:methods; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D010316:Particle Size; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D011189:Potassium Chloride / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000596:Amino Acids; D010455:Peptides; D011189:Potassium Chloride