In vitro effect of zinc and cadmium on acetylcholinesterase and ectonucleotidase activities in zebrafish (Danio rerio) brain.
Zinc and cadmium are environmental contaminants that induce a wide range of effects on CNS. Here we tested the in vitro effect of these metals on acetylcholinesterase (AChE) and ectonucleotidase (NTPDase and ecto-5'-nucleotidase) activities in zebrafish brain. Both zinc and cadmium treatments did not alter significantly the zebrafish brain AChE activity. ATP hydrolysis presented a significant increase at 1 mM zinc (17%) and the AMPase activity had a dose-dependent increase at 0.5 and 1 mM zinc exposure (188% and 199%). After cadmium treatment, ATPase activity was significantly increased (53% and 48%) at 0.5 and 1 mM, respectively. Cadmium, in the range 0.25-1 mM, inhibited ADP hydrolysis in a dose-dependent manner (13.4-69%). Ecto-5'-nucleotidase activity was only inhibited (38%) in the presence of 1 mM cadmium. It is possible to suggest that changes on NTPDase and ecto-5'-nucleotidase activities can be an important mechanism involved in neurotoxic effects promoted by zinc and cadmium.
Senger
Mario Roberto
MR
Laboratório de Pesquisa Bioquímica, Departamento de Ciências Fisiológicas, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga, 6681, 90619-900 Porto Alegre, RS, Brazil.
Rosemberg
Denis Broock
DB
Rico
Eduardo Pacheco
EP
de Bem Arizi
Marcelo
M
Dias
Renato Dutra
RD
Bogo
Maurício Reis
MR
Bonan
Carla Denise
CD
D015720:5'-Nucleotidase / Q000378:metabolism*; D000110:Acetylcholinesterase / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002104:Cadmium / Q000633:toxicity*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009708:Nucleotidases / Q000378:metabolism; D015027:Zebrafish; D015032:Zinc / Q000633:toxicity*
D002104:Cadmium; D000110:Acetylcholinesterase; D009708:Nucleotidases; C018775:adenosine monophosphatase; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases; D015032:Zinc