title: [Analysis of proteins in urinary tract stones and urine of urolithic patients].
abstract: In order to investigate the mechanism of urinary tract stone formation, we analyzed protein components in urine and the stone. Urinary proteins of healthy subjects and urolithic patients as well as protein components urinary tract stone of the urolithic patients were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Electrophoretic patterns of urinary proteins of the patients differed from those of healthy subjects after separating protein patterns into those larger than 66kDa or smaller than 30kDa. Protein constituents of urinary tract stone were mainly separated into 18 bands ranging from 26.8 to 143 kDa. Major bands among these 18 bands differed among stones from different patients. On western blotting, the developed intensities of Tamm-Horsfall protein (THP) were fainter than those of healthy subjects. Whereas intensities of albumin (ALB) were stronger than those of healthy subjects. Moreover, blotting patterns of THP of the patients on non-reducing SDS-PAGE were obviously broad. Thus, we suggest that analysis of fractionated urinary proteins or protein components of urinary tract stone may provide a tool for monitoring the prognosis or relapse in the patients.
authors.affiliation: Analytical Laboratory Chemistry, Graduate School of Allied Health Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8519.
authors.forename: Kayo
authors.initials: K
authors.lastname: Yokomizoi
authors.forename: Aki
authors.initials: A
authors.lastname: Nakayama
authors.forename: Eisaku
authors.initials: E
authors.lastname: Hokazono
authors.forename: Akiko
authors.initials: A
authors.lastname: Ninomiya
authors.forename: Ruriko
authors.initials: R
authors.lastname: Miyake
authors.forename: Nobuo
authors.initials: N
authors.lastname: Hiratsuka
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Okuyama
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kato
authors.forename: Shizuko
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Ito
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Shiba
mesh_terms: D000328:Adult; D000368:Aged; D000418:Albumins / Q000032:analysis; D000419:Albuminuria / Q000652:urine; D015415:Biomarkers / Q000652:urine; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009088:Mucoproteins / Q000032:analysis / Q000652:urine; D011506:Proteins / Q000032:analysis*; D011507:Proteinuria / Q000652:urine*; D012967:Sodium Dodecyl Sulfate; D014545:Urinary Calculi / Q000737:chemistry* / Q000175:diagnosis; D058949:Uromodulin
chemical_list: D000418:Albumins; D015415:Biomarkers; D009088:Mucoproteins; D011506:Proteins; C548612:UMOD protein, human; D058949:Uromodulin; D012967:Sodium Dodecyl Sulfate