title: Thorsellia anophelis gen. nov., sp. nov., a new member of the Gammaproteobacteria.
abstract: A Gram-negative, rod-shaped organism (CCUG 49520T) was isolated from the midgut of the mosquito Anopheles arabiensis. 16S rRNA gene sequence analysis demonstrated that this isolate is unique, showing <93% similarity to species of the families Enterobacteriaceae and Vibrionaceae. The quinone system consisted exclusively of ubiquinone Q-8; the polar lipid profile consisted of the major compounds phosphatidylethanolamine and phosphatidylglycerol, a moderate to minor amount of two unknown aminophospholipids, an unknown phospholipid and two unknown polar lipids; the polyamine pattern was characterized by the predominant compound 1,3-diaminopropane and showed some significant differences when compared with members of the Enterobacteriaceae and Vibrionaceae. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic data, strain CCUG 49520T is considered to represent a new genus and species, for which the name Thorsellia anophelis gen. nov., sp. nov. is proposed. The type strain is CCUG 49520T (=CIP 108754T).
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, IFZ-Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Department of Genetics, Microbiology and Toxicology, S-106 91 Stockholm University, Stockholm, Sweden.
authors.forename: Jenny M
authors.initials: JM
authors.lastname: Lindh
authors.affiliation: Department of Genetics, Microbiology and Toxicology, S-106 91 Stockholm University, Stockholm, Sweden.
authors.forename: Olle
authors.initials: O
authors.lastname: Terenius
authors.affiliation: Department of Genetics, Microbiology and Toxicology, S-106 91 Stockholm University, Stockholm, Sweden.
authors.forename: Siamak
authors.initials: S
authors.lastname: Haghdoost
authors.affiliation: CCUG, Culture Collection University of Göteborg, Göteborg, Sweden.
authors.forename: Enevold
authors.initials: E
authors.lastname: Falsen
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: Department of Genetics, Microbiology and Toxicology, S-106 91 Stockholm University, Stockholm, Sweden.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Faye
mesh_terms: D000818:Animals; D000852:Anopheles / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004064:Digestive System / Q000382:microbiology; D004755:Enterobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology; D020563:Gammaproteobacteria / Q000145:classification*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S