Characterization of severe acute respiratory syndrome coronavirus membrane protein.
The coronavirus membrane protein (M) is the key player in the assembly of virions at intracellular membranes between endoplasmic-reticulum and Golgi-complex. Using a newly established human monoclonal anti-M antibody we detected glycosylated and nonglycosylated membrane-associated M in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infected cells and in purified virions. Further analyses revealed that M contained a single N-glycosylation site at asparagine 4. Recombinant M was transported to the plasma membrane and gained complex-type N-glycosylation. In SARS-CoV infected cells and in purified virions, however, N-glycosylation of M remained endoglycosidase H-sensitive suggesting that trimming of the N-linked sugar side chain is inhibited.
Voss
Daniel
D
Institute for Virology, Philipps-University Marburg, Robert-Koch-Str. 17, 35037 Marburg, Germany.
Kern
Anika
A
Traggiai
Elisabetta
E
Eickmann
Markus
M
Stadler
Konrad
K
Lanzavecchia
Antonio
A
Becker
Stephan
S
D000818:Animals; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D000086862:Coronavirus M Proteins; D006031:Glycosylation; D006801:Humans; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D021381:Protein Transport / Q000502:physiology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000378:metabolism*; D045169:Severe Acute Respiratory Syndrome / Q000235:genetics / Q000378:metabolism / Q000821:virology; D014709:Vero Cells; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*
D000086862:Coronavirus M Proteins; C000706237:M protein, SARS-CoV; D008565:Membrane Proteins; D014763:Viral Matrix Proteins; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase