The radiation sensitivity of human chromosomes 2, 8 and 14 in peripheral blood lymphocytes of seven donors.
OBJECTIVE
To investigate if deviations from DNA-proportional distribution of radiation-induced chromosomal aberrations are individually variable.


METHODS
Peripheral blood lymphocytes were collected from seven healthy donors and exposed to different doses of gamma rays. Chromosomes 2, 8 and 14 were painted in different colors and aberrations scored with the help of an image-analysis system.


RESULTS
Chromosome 2 was generally less sensitive than expected on the basis of DNA-proportional distribution and the extent of inter-donor variability was minimal. A higher than expected frequency of aberrations was found in chromosome 14 of five donors, while a higher than expected frequency of aberrations was found in chromosome 8 of two donors.


CONCLUSIONS
Inter-donor variability may explain some of the controversies regarding the inter-chromosomal distribution of radiation-induced aberrations.
Sommer
Sylwester
S
Institute of Nuclear Chemistry and Technology, Department of Radiobiology and Health Protection, Warszawa.
Buraczewska
Iwona
I
Wojewodzka
Maria
M
Bouzyk
Elzbieta
E
Szumiel
Irena
I
Wojcik
Andrzej
A
D000328:Adult; D018929:Cell Culture Techniques; D002869:Chromosome Aberrations / Q000528:radiation effects*; D002883:Chromosomes, Human, Pair 14 / Q000528:radiation effects*; D002889:Chromosomes, Human, Pair 2 / Q000528:radiation effects*; D002898:Chromosomes, Human, Pair 8 / Q000528:radiation effects*; D004247:DNA / Q000032:analysis; D004249:DNA Damage; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results
D004247:DNA