Saccharomyces cerevisiae mitoproteome plasticity in response to recombinant alternative ubiquinol oxidase.
The energy-dissipating alternative oxidase (AOX) from Hansenula anomala was expressed in Saccharomyces cerevisiae. The recombinant AOX was functional. A comparative analysis by two-dimensional differential in-gel electrophoresis (2D-DIGE) of mitochondrial protein patterns found in wild-type and recombinant AOX strains was performed. 60 proteins exhibiting a significant difference in their abundance were identified. Interestingly, proteins implicated in major metabolic pathways such as Krebs cycle and amino acid biosynthesis were up-regulated. Surprisingly, an up-regulation of the respiratory-chain complex III was associated with a down-regulation of the ATP synthase complex.
Mathy
Grégory
G
Laboratory of Bioenergetics, Bât. B6c, Allée de la Chimie 3, 4000, Liège, Belgium.
Navet
Rachel
R
Gerkens
Pascal
P
Leprince
Pierre
P
De Pauw
Edwin
E
Sluse-Goffart
Claudine M
CM
Sluse
Francis E
FE
Douette
Pierre
P
D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D013058:Mass Spectrometry; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010101:Oxygen Consumption; D010843:Pichia / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D000099188:Alternative Oxidase
D005656:Fungal Proteins; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D011994:Recombinant Proteins; D000099188:Alternative Oxidase