title: Cell column chromatography: a new research tool to quantify cerebral cell volume changes following chemically-induced anoxia/re-oxygenation.
abstract: OBJECTIVE
  The roles of individual types of cerebral cells in contributing to brain edema are undefined. The objective of this study was to determine the role of cerebral cell-column chromatography in quantifying cell volumes of individual cerebral cell lines, under chemically-induced anoxia/re-oxygenation (A/R).
  
  
  METHODS
  Cerebral endothelial cells (4 experiments) or type II astrocytes (4 experiments) were cultured to confluence on microcarrier beads. A chromatographic cell-column of 1.5 cm height was filled with non-treated cell-covered beads. The column was perfused at 1 ml/min with a balanced perfusate for one hour (Baseline). The perfusate was then switched to that containing 5 mM thioglycolic acid for one hour (Anoxia). Then the column was perfused with the normal perfusate for another two hours (Re-oxygenation). The total free space in the column, reversely reflecting cell volumes, was determined by averaged transit time (TTa) of a non-permeable flow tracer blue dextran. Decreased TTa means that cells swell, and vice versa.
  
  
  RESULTS
  TTa in endothelial cell columns increased with a peak at 60 minutes of re-oxygenation. TTa in astrocyte columns decreased with a nadir at 30 minutes of re-oxygenation.
  
  
  CONCLUSIONS
  Cell column chromatography can be used to determine the cerebral cell volume changes following chemically-induced anoxia/re-oxygenation.
authors.affiliation: Department of Emergency Medicine, Louisiana State University Health Science Center, Shreveport 71130, USA. fxiao@lsuhsc.edu
authors.forename: F
authors.initials: F
authors.lastname: Xiao
authors.forename: S
authors.initials: S
authors.lastname: Pardue
authors.forename: T
authors.initials: T
authors.lastname: Nash
authors.forename: T C
authors.initials: TC
authors.lastname: Arnold
authors.forename: J S
authors.initials: JS
authors.lastname: Alexander
authors.forename: D L
authors.initials: DL
authors.lastname: Carden
authors.forename: R
authors.initials: R
authors.lastname: Turnage
authors.forename: A
authors.initials: A
authors.lastname: Jawahar
authors.forename: S A
authors.initials: SA
authors.lastname: Conrad
mesh_terms: D001929:Brain Edema / Q000139:chemically induced / Q000473:pathology*; D002452:Cell Count / Q000379:methods; D002469:Cell Separation / Q000379:methods*; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D002845:Chromatography / Q000379:methods*; D042783:Endothelial Cells / Q000187:drug effects / Q000473:pathology*; D006801:Humans; D015427:Reperfusion Injury / Q000139:chemically induced / Q000473:pathology*; D013864:Thioglycolates
chemical_list: D013864:Thioglycolates