title: Tannins from barks of Pinus caribaea protect Escherichia coli cells against DNA damage induced by gamma-rays.
abstract: This work was aimed to evaluate genotoxicity and antigenotoxicity activity against gamma-rays of a tannin fraction obtained from barks of Pinus caribaea, as well as to elucidate the antigenotoxic mechanisms involved in radioprotection by using different approaches as pre-, co- and post-irradiation cell treatments with plant extract. The tannin fraction was not genotoxic to Escherichia coli cells in experiments using different exposure times. This extract was antigenotoxic against gamma-rays when the cells were pre- or co-treated with this extracts, but not during post-irradiation treatments, suggesting a possibly antigenotoxic action through free radical scavenging mechanisms. The results are discussed in relation to the chemopreventive and therapeutic potential of the studied plant species.
authors.affiliation: Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN), Miramar, Playa, Ciudad de la Habana, Cuba. jlfuentes2005@yahoo.es
authors.forename: J L
authors.initials: JL
authors.lastname: Fuentes
authors.forename: M
authors.initials: M
authors.lastname: Vernhe
authors.forename: E B
authors.initials: EB
authors.lastname: Cuetara
authors.forename: A
authors.initials: A
authors.lastname: Sánchez-Lamar
authors.forename: J L
authors.initials: JL
authors.lastname: Santana
authors.forename: M
authors.initials: M
authors.lastname: Llagostera
mesh_terms: D004249:DNA Damage / Q000187:drug effects*; D004269:DNA, Bacterial / Q000187:drug effects / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D005720:Gamma Rays; D009152:Mutagenicity Tests / Q000379:methods; D028223:Pinus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D011837:Radiation-Protective Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D004269:DNA, Bacterial; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D013634:Tannins