Jagged-1hi or Delta-1hi thymic stromal cell populations can trigger acquisition of Thy-1 and loss of B220.
Effects of endogenous Notch ligands Jagged-1 and Delta-1 on maturation markers in adult mouse lymphocytes were checked in vitro on putative thymocyte precursors from bone marrow and on DN3 cells from the thymus. Both populations were cocultured with stromal cells (Small M, Histochem Cell Biol 2005;123:513) naturally expressing high levels of either Jagged-1 or Delta-1. Initially the bone marrow cells were Thy-1.2(neg) while the majority were B220(hi). These trends were reversed by culture on both the Jagged-1(hi) and Delta-1(hi) monolayers. Then DN3 thymocytes were cultured with stroma expressing these two ligands and the T cell receptor beta chain was expressed more clearly after coculture on Delta-1(hi) stroma. These findings indicate that stromal cells with endogenous Jagged-1 as well as Delta-1 could initiate early changes toward T cell commitment while Delta-1 favored later alphabeta-TCR development.
Small
Myra
M
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel. msmall@post.tau.ac.il
D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002460:Cell Line; D018920:Coculture Techniques; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008809:Mice, Inbred C3H; D000072099:Serrate-Jagged Proteins; D013234:Stem Cells / Q000378:metabolism / Q000648:ultrastructure; D017154:Stromal Cells / Q000378:metabolism / Q000648:ultrastructure; D018800:Thy-1 Antigens / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; C000606274:Jag1 protein, mouse; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D000072099:Serrate-Jagged Proteins; D018800:Thy-1 Antigens; C064045:delta protein; D017493:Leukocyte Common Antigens