Applications of LC-MS in the study of the uptake, distribution, metabolism and excretion of bioactive polyphenols from dietary supplements.
Specific and quantitative analyses of the bioactive components and their metabolites in body fluids are essential to assess the interaction between groups of compounds in dietary supplements and preparations of psychoactives. Reverse-phase LC separations combined with tandem mass spectrometry provide the necessary specificity and sensitivity. In this paper, applications of these methods are described for the analysis of isoflavones, salvinorin A, synephrine isomers and their metabolites in serum, urine and aqueous humor.
Barnes
Stephen
S
Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA. sbarnes@uab.edu
Prasain
Jeevan K
JK
Wang
Chao-Cheng
CC
Moore
D Ray
DR
D000818:Animals; D002853:Chromatography, Liquid; D019587:Dietary Supplements; D004224:Diterpenes / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics; D045785:Diterpenes, Clerodane; D004285:Dogs; D005419:Flavonoids / Q000737:chemistry* / Q000378:metabolism* / Q000493:pharmacokinetics; D013058:Mass Spectrometry; D010636:Phenols / Q000737:chemistry* / Q000378:metabolism* / Q000493:pharmacokinetics; D059808:Polyphenols; D011619:Psychotropic Drugs / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013578:Synephrine / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics
D004224:Diterpenes; D045785:Diterpenes, Clerodane; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D011619:Psychotropic Drugs; D013578:Synephrine; C090499:salvinorin A