Expression of cyclin H in normal and cancerous endometrium, its correlation with other cyclins, and association with clinicopathologic parameters.
Cyclins are known as regulatory proteins in cell cycle. Cyclin H is a part of cyclin H/Cdk7/Mat1 complex, which is necessary for cellular proliferation. This study was designed to investigate the correlation of cyclin H expression with tumorigenesis of the endometrium and clinicopathologic variables. Immunohistochemical staining using labeled streptavidin-biotin complex was performed on formalin-fixed, paraffin-embedded endometrial tissues of the proliferative, hyperplastic, and carcinomatous types. Immunostaining for cyclins A, B1, D1, D3, E, H, and cyclin dependent kinase 2 were evaluated. The expression of cyclins A, D1, D3, and H in hyperplasia was significantly more frequent than those of proliferative phase and less than those of endometrioid adenocarcinoma. The expression of cyclin H was correlated with lymphvascular space invasion and clinical stage in carcinoma but not with myometrial invasion, lymph node metastasis, and menopause status. The expression of cyclin H could be involved in the transformation of the endometrium into malignancy and might be a marker for more proliferative and malignant features. It might be one of the biomarkers for determining proliferative activity in endometrial hyperplasia and endometrioid adenocarcinoma.
Kayaselcuk
F
F
Department of Pathology, Adana Research and Medical Center, Baskent University, Yuregir 01350, Adana, Turkey. faziletks@yahoo.com
Erkanli
S
S
Bolat
F
F
Seydaoglu
G
G
Kuscu
E
E
Demirhan
B
B
D014408:Biomarkers, Tumor / Q000032:analysis*; D001707:Biopsy, Needle; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000032:analysis*; D019925:Cyclin A / Q000032:analysis; D019926:Cyclin B / Q000032:analysis; D019938:Cyclin D1 / Q000032:analysis; D019927:Cyclin E / Q000032:analysis; D056748:Cyclin H; D016213:Cyclins / Q000032:analysis*; D003937:Diagnosis, Differential; D004714:Endometrial Hyperplasia / Q000473:pathology*; D016889:Endometrial Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009367:Neoplasm Staging; D011336:Probability; D012016:Reference Values; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D046509:Tissue Culture Techniques
D014408:Biomarkers, Tumor; C534751:CCNH protein, human; D018797:Cell Cycle Proteins; D019925:Cyclin A; D019926:Cyclin B; D019927:Cyclin E; D056748:Cyclin H; D016213:Cyclins; D019938:Cyclin D1