[Effect of a hypothetical gene Af116609 on multi-drug resistance of gastric cancer cells].
OBJECTIVE
To investigate the effect of gene Af116609 on gastric cancer multi-drug resistance (MDR) by introducing it into gastric cancer multi-drug resistant (MDR) cell line SGC7901/VCR.


METHODS
Gene Af116609 was cloned from SGC7901/VCR by RT-PCR and its differential expression between gastric cancer MDR cells and its parental cells was displayed by Northern blot. The gene was introduced to gastric cancer cells by transfection of recombinant eukaryotic expression vector by electroporation. MTT assay in vitro was applied to investigate its effect on multi-drug resistance phenotype of gastric cancer cells.


RESULTS
The full length CDS of gene Af116609, as long as 327 bp, was cloned from gastric cancer MDR cell line SGC7901/VCR and its sequence was coincident with the hypothetical gene Af116609 in GenBank. It was overexpressed in MDR cells than its parental cells at mRNA level. In the MTT assay in vitro, the drug sensitive cells transfected with sense eukaryotic expression vector showed upregulated targeted gene, with increased resistance to vincristine, 5-fliorouracil and arabinoside, and decreased resistance to adriamycin, but no influence on resistance to methotrexate. However, the drug resistant cells transfected with anti-sense eukaryotic expression vector, showed down regulated targeted gene, with less resistance to all the five anticancer drugs to different degrees.


CONCLUSIONS
Gene Af116609 is related to MDR phenotype of gastric cancer cells and may become a candidate molecular target to reverse the MDR of gastric cancer.
Jin
Xiao-hang
XH
Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Du
Jing-ping
JP
Yin
Fang
F
Zhang
Yu-mei
YM
Hu
Wen-hua
WH
Cao
Yun-xin
YX
Shi
Yong-quan
YQ
Zhao
Yan-qiu
YQ
Qiao
Tai-dong
TD
Fan
Dai-ming
DM
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001324:Autoantigens / Q000235:genetics*; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D006801:Humans; D020733:RNA, Small Cytoplasmic / Q000235:genetics*; D012261:Ribonucleoproteins / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis; D014750:Vincristine / Q000494:pharmacology*; D000099207:SS-A Antigen
D000972:Antineoplastic Agents, Phytogenic; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D042461:Vascular Endothelial Growth Factor A; D014750:Vincristine; C116006:RO60 protein, human; D000099207:SS-A Antigen