Novel chemically synthesized salicylate derivative as an effective elicitor for inducing the biosynthesis of plant secondary metabolites.
Trifluoroethyl salicylate (TFESA), a novel salicylate derivative, was chemically synthesized and evaluated by bioassay as a potential elicitor for inducing the biosynthesis of plant secondary metabolites. A cell line of Taxus chinensis, which stably produced a high level of bioactive taxuyunnanine C (Tc), was taken as a model plant cell system. The application of TFESA to the cell cultures could significantly induce Tc biosynthesis, although the cell growth was slightly inhibited. More interestingly, Tc production was enhanced more in the presence of TFESA compared with a structure-similar well-known elicitor, salicylic acid (SA). For example, addition of 100 microM TFESA on day 7 led to a high Tc content of 21.9 +/- 0.1 mg g(-1) (at day 21), whereas the Tc content was 14.0 +/- 0.2 and 16.7 +/- 0.3 mg g(-1) for the control and that with addition of 100 microM SA, respectively. The results indicate that the newly synthesized TFESA can act as a powerful elicitor for secondary metabolism induction in plant cell cultures.
Qian
Zhi-Gang
ZG
State Key Laboratory of Bioreactor Engineering, Shanghai 200237, China. littlemony@sina.com
Zhao
Zhen-Jiang
ZJ
Xu
Yufang
Y
Qian
Xuhong
X
Zhong
Jian-Jiang
JJ
D001681:Biological Assay; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D007700:Kinetics; D008954:Models, Biological; D015394:Molecular Structure; D012459:Salicylates / Q000138:chemical synthesis* / Q000494:pharmacology*; D043823:Taxoids / Q000378:metabolism*; D020946:Taxus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D012459:Salicylates; D043823:Taxoids; C494600:taxuyunnanine C; C510254:trifluoroethyl salicylate