title: A catalog for transcripts in the venom gland of the Agkistrodon acutus: identification of the toxins potentially involved in coagulopathy.
abstract: Agkistrodon acutus is a special agkistrodon halys, only distributed in Southern China, with a few exceptions in Vietnam. It is a cherished element used in traditional Chinese medicine. In order to produce a global panorama of gene expression in the Agkistrodon acutus venom gland, a non-normalized cDNA library was constructed, and 8696 high quality 5' end expressed sequenced tags (ESTs) were sequenced and analyzed. The initial sequences were assembled into 2855 clusters. Of these clusters, only 45.60% clusters matched known sequence and 54.40% had no match to any known sequence in GenBank. Except for putative cellular proteins (1184 clusters), the remaining 118 clusters (40.16% of all ESTs) corresponded to sequences associated with diverse toxin function. According to expression abundance, the major toxin components were metalloproteinases (32.08%) and C-type lectin (5.22%), and other components including bradykinin-potentiating peptide (0.90%), serine proteases (0.51%), nucleotidase and nuclease (0.41%), phospholipase A2 (0.30%), disintegrin (0.05%), cytokine-like molecules (0.06%), and other proteins (0.63%). The majority of these components are thought to be responsible for coagulopathy after A. acutus bites. We have therefore generated a comprehensive catalog of the A. acutus venom gland described so far. Gene expression from the very specialized secretory tissue, especially for those involved in coagulopathy, can be surveyed and provide important information in finding novel toxins.
authors.affiliation: Department of Pharmacology, Zhong-Shan Medical College, Sun Yat-sen University, Guangzhou 510089, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Qinghua
authors.forename: Zhang
authors.initials: Z
authors.lastname: Xiaowei
authors.forename: Yin
authors.initials: Y
authors.lastname: Wei
authors.forename: Li
authors.initials: L
authors.lastname: Chenji
authors.forename: Huang
authors.initials: H
authors.lastname: Yijun
authors.forename: Qiu
authors.initials: Q
authors.lastname: Pengxin
authors.forename: Su
authors.initials: S
authors.lastname: Xingwen
authors.forename: Hu
authors.initials: H
authors.lastname: Songnian
authors.forename: Yan
authors.initials: Y
authors.lastname: Guangmei
mesh_terms: D017836:Agkistrodon / Q000378:metabolism*; D000818:Animals; D001777:Blood Coagulation; D016000:Cluster Analysis; D019295:Computational Biology; D018076:DNA, Complementary / Q000378:metabolism; D020224:Expressed Sequence Tags; D015723:Gene Library; D037181:Lectins, C-Type / Q000737:chemistry; D045726:Metalloproteases / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012910:Snake Venoms / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D037181:Lectins, C-Type; D012333:RNA, Messenger; D012910:Snake Venoms; D045726:Metalloproteases