Glial fibrillary acidic protein expression after electroconvulsive shocks in rat brain.
OBJECTIVE
The aim of the present study was to assess the effect of electroconvulsive shock (ECS) in glial fibrillary acidic protein (GFAP) expression in rat brain.


METHODS
Rats were given either a single (acute) or a series of eight (chronic) ECS. Brain regions were isolated and levels of glial fibrillary acidic protein (GFAP) in the brain tissue (cortex, hippocampus, and cerebellum) were assessed using an enzyme-linked immunosorbent assay (ELISA).


RESULTS
We showed that GFAP expression is reduced in the hippocampus within 48 h and 7 days after acute ECS. GFAP levels are increased in the cerebellum immediately after acute and chronic ECS. No changes were observed in the cortex.


CONCLUSIONS
Our findings showed a differential effect of acute and chronic ECS in the astroglial response in the brain of rats.
Ceresér
Keila M
KM
Laboratório de Psiquiatria Experimental, Centro de Pesquisas, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2350, 90035-003 Porto Alegre, RS, Brazil.
Frey
Benício N
BN
Bernardes
Fernanda B
FB
Costa
Sabrina C
SC
Andreazza
Ana C
AC
Feier
Gustavo
G
Souza
Daniela
D
Tramontina
Francine
F
Gonçalves
Carlos A
CA
Kapczinski
Flávio
F
Quevedo
João
J
D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000528:radiation effects*; D004597:Electroshock; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015870:Gene Expression / Q000528:radiation effects*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D005904:Glial Fibrillary Acidic Protein