The effect of high-fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3-ablated mice.
Uncoupling protein-3 (UCP3) has been suggested to protect against lipid-induced oxidative damage. Therefore, we studied intramuscular lipid peroxide levels and high-fat diet induced alterations in muscle lipid metabolism of UCP3-ablated mice. UCP3-/- mice showed approximately 3-fold higher levels of intramuscular lipid peroxides upon standard chow feeding, compared to wild-type littermates. Remarkably, this difference was no longer apparent on the high-fat diet. However, upon high-fat feeding, intramuscular triacylglycerol levels were approximately 50% lower in UCP3-/- mice, in comparison to UCP3+/+ animals. Succinate dehydrogenase activity, and total protein content of the muscle fatty acid transporter FAT/CD36 were however similar between UCP3-/- and UCP3+/+ mice.
Hoeks
Joris
J
Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands. j.hoeks@hb.unimaas.nl
Hesselink
Matthijs K C
MK
Sluiter
Wim
W
Schaart
Gert
G
Willems
Jodil
J
Morrisson
Alastair
A
Clapham
John C
JC
Saris
Wim H M
WH
Schrauwen
Patrick
P
D000818:Animals; D018955:CD36 Antigens / Q000032:analysis; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D004041:Dietary Fats / Q000494:pharmacology*; D007473:Ion Channels; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008055:Lipids / Q000032:analysis; D051379:Mice; D018345:Mice, Knockout; D024101:Mitochondrial Proteins; D009132:Muscles / Q000737:chemistry / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014280:Triglycerides / Q000032:analysis; D000071247:Uncoupling Protein 3
D018955:CD36 Antigens; D002352:Carrier Proteins; D004041:Dietary Fats; D007473:Ion Channels; D008055:Lipids; D024101:Mitochondrial Proteins; D014280:Triglycerides; C000604392:UCP3 protein, human; C000604393:Ucp3 protein, mouse; D000071247:Uncoupling Protein 3; D013385:Succinate Dehydrogenase