title: Conserved cis-elements in the Xenopus red opsin promoter necessary for cone-specific expression.
abstract: The long-wavelength sensitive (red) opsin genes encode proteins which play a central role in daytime and color vision in vertebrates. We used transgenic Xenopus to identify 5' cis-elements in the red cone opsin promoter necessary for cone-specific expression. We found a highly conserved extended region (-725 to -173) that was required for restricting GFP transgene expression to cones. We further identified a short element (5'-CCAATTAAGAGAT-3') highly conserved amongst tetrapods, including humans, necessary to restrict expression to cones in the retina. These results identify novel conserved elements that regulate spatial expression of tetrapod red cone opsin genes.
authors.affiliation: Department of Biochemistry & Molecular Biology and Ophthalmology, 750 E. Adams Street, SUNY Upstate Medical University, Syracuse, NY 13210, USA.
authors.forename: S
authors.initials: S
authors.lastname: Babu
authors.forename: V
authors.initials: V
authors.lastname: McIlvain
authors.forename: S L
authors.initials: SL
authors.lastname: Whitaker
authors.forename: B E
authors.initials: BE
authors.lastname: Knox
mesh_terms: D024506:5' Flanking Region; D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D017124:Conserved Sequence; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D011401:Promoter Regions, Genetic; D017949:Retinal Cone Photoreceptor Cells; D017299:Rod Opsins / Q000235:genetics*; D014982:Xenopus laevis
chemical_list: D017299:Rod Opsins