title: [An experimental study on transplantation of frozen canine phalangeal joint allografts incorporated with autogenic bone marrow].
abstract: OBJECTIVE
  To investigate the result of the transplantation of frozen canine phalangeal joint allografts perforated and incorporated with autogenic bone marrow.
  
  
  METHODS
  A proximal interphalangeal joint defect of 1.5 cm was prepared at bilateral sides of twenty-four adult healthy out-bred dogs. Three different types of allografts were applied to repair the defects: fresh autogenic phalangeal joints (group A, n = 16), frozen phalangeal joint allografts perforated and incorporated with fresh autogenic bone marrow (group B, n = 16), and frozen phalangeal joint allografts (group C, n = 16). Radiographic and histological study were used to evaluate the survival of transplanted joints. The observation was done 1, 3, 6 and 12 months after operation respectively.
  
  
  RESULTS
  Based on the radiographic and histological changes of the transplanted joints, the osteoarthropathy of transplanted canine phalangeal joints could be divided into 3 degrees: mild degeneration, moderate degeneration and severe degeneration. Mild degeneration was observed in group A from 3 to 12 months. Mild degeneration was also found in group B from 1 to 6 months, and the endochondral ossification was obvious within the drilled bony holes. However, some joints in group B underwent moderate degeneration 12 months after operation. Group C joints in the first month had moderate degeneration, which progressed to severe degeneration 3 months after operation.
  
  
  CONCLUSIONS
  Transplantation of frozen canine phalangeal joint allografts perforated and incorporated with autogenic bone marrow can effectively delay the degeneration of transplanted osteoarticular allografts at the early and middle stage.
authors.affiliation: Department of Plastic Surgery, First Affiliated People's Hospital, Shanghai Jiaotong University, Shanghai 200080, PR China. zlshen@sh163.net
authors.forename: Zunli
authors.initials: Z
authors.lastname: Shen
authors.forename: Wanxin
authors.initials: W
authors.lastname: Jia
authors.forename: Zhaofeng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Shen
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Wang
authors.forename: Kaiheng
authors.initials: K
authors.lastname: Zhang
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Hou
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D016025:Bone Transplantation / Q000379:methods*; D015925:Cryopreservation; D004195:Disease Models, Animal; D004285:Dogs; D033023:Foot Joints / Q000637:transplantation*; D014033:Toe Joint / Q000293:injuries; D019172:Transplantation Conditioning; D014182:Transplantation, Autologous