title: High-performance liquid chromatography of lactose with evaporative light scattering detection, applied to determine fine particle dose of carrier in dry powder inhalation products.
abstract: A method for quantification of the fine particle dose of lactose is described, using a hydrophilic interaction chromatography (HILIC) method and evaporative light scattering detection. The HILIC method used an aminopropyl column and a mobile phase consisting of acetonitril/water (80/20, v/v) for isocratic elution. Sensitive chromatography was obtained using a low concentration of water in the extraction solvent. The detection limit (RSD<10%) at an injection volume of 10 microL is 10 microg/mL. Linearity was obtained in the range of 10-80 microg/mL (R(2)>0.99). A relative standard deviation (RSD) of 0.5% (N=6) demonstrated good precision of the optimized method.
authors.affiliation: Institute of Pharmacy, Johannes Gutenberg University, 55099 Mainz, Germany.
authors.forename: Bianca
authors.initials: B
authors.lastname: Beilmann
authors.forename: Peter
authors.initials: P
authors.lastname: Langguth
authors.forename: Heribert
authors.initials: H
authors.lastname: Häusler
authors.forename: Peter
authors.initials: P
authors.lastname: Grass
mesh_terms: D000280:Administration, Inhalation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007785:Lactose / Q000032:analysis*; D008027:Light; D011208:Powders / Q000737:chemistry*; D015203:Reproducibility of Results; D012542:Scattering, Radiation
chemical_list: D011208:Powders; D007785:Lactose