title: Priming type II polyketide synthases via a type II nonribosomal peptide synthetase mechanism.
abstract: Benzoic acid priming of the enterocin and actinorhodin type II polyketide synthase complexes was accomplished in vitro via an unprecedented type II nonribosomal peptide synthetase-like mechanism involving the benzoate:acyl carrier protein (ACP) ligase EncN and the ACP EncC. The transfer of the aryl acid to the ACP is ATP-dependent, yet coenzyme A-independent, as characterized with radiolabeled substrates and protein mass spectrometry. Subsequent transport of the ACP-bound aryl group to the native enterocin and the aberrant actinorhodin ketosynthase chain length factor heterodimers was further demonstrated, thereby demonstrating the potential of this biocatalyst for engineering diverse aryl-primed aromatic polyketide agents.
authors.affiliation: College of Pharmacy, University of Arizona, Tucson, Arizona 85721, USA.
authors.forename: Miho
authors.initials: M
authors.lastname: Izumikawa
authors.forename: Qian
authors.initials: Q
authors.lastname: Cheng
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Moore
mesh_terms: D000213:Acyl Carrier Protein / Q000737:chemistry / Q000378:metabolism; D000880:Anthraquinones / Q000737:chemistry / Q000378:metabolism; D003065:Coenzyme A / Q000737:chemistry / Q000378:metabolism; D009279:Naphthacenes / Q000737:chemistry / Q000378:metabolism; D010453:Peptide Synthases / Q000737:chemistry / Q000378:metabolism*; D048630:Polyketide Synthases / Q000737:chemistry / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000213:Acyl Carrier Protein; D000880:Anthraquinones; D003065:Coenzyme A; D009279:Naphthacenes; D010453:Peptide Synthases; D048630:Polyketide Synthases; C080889:5838 DNI; C104385:non-ribosomal peptide synthase; C013390:actinorhodin; D000095622:Benzoisochromanequinones