[Purification and analysis of polysaccharides from the caudex of Undaria pinnatifida].
To isolate polysaccharides with hot water from the caudex of Undaria pinnatifida, and precipitate with ethanol. The protein in polysaccharides was removed by sevage way. DEAE-52 and Sephadex G-200 column chromatography were used to isolate and purification polysaccharides, three purified polysaccharides F2, F3 and F4 were obtained. It was identified that they were homogeneity. The ultraviolet spectrum showed there was no proteins and nucleic acids in F2, F3 and F4. Through thin-layer chromatography analysis, F2, F3 and F4 were mainly composed of Gal, Fuc, Man and Glu acid. F2 also contained Glu and Rha.
Kang
Yanyan
Y
Jinan University Biomedicine Research & Development Center,Guangzhou 510632.
Wang
Yifei
Y
Zhu
Liang
L
Men
Xiaoyuan
X
D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D008358:Mannose / Q000032:analysis; D009005:Monosaccharides / Q000032:analysis*; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D044648:Undaria / Q000737:chemistry*
D009005:Monosaccharides; D011134:Polysaccharides; D005643:Fucose; D008358:Mannose; D005690:Galactose