title: Parallel picoliter rt-PCR assays using microfluidics.
abstract: The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis.
authors.affiliation: Option in Biochemistry and Molecular Biophysics, Department of Applied Physics, California Institute of Technology, Pasadena, 91125, USA.
authors.forename: Joshua S
authors.initials: JS
authors.lastname: Marcus
authors.forename: W French
authors.initials: WF
authors.lastname: Anderson
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Quake
mesh_terms: D006801:Humans; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D012313:RNA / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D012313:RNA