Molecular cloning and characterization of a vacuolar H+ -pyrophosphatase gene, SsVP, from the halophyte Suaeda salsa and its overexpression increases salt and drought tolerance of Arabidopsis.
The chenopodiaceae Suaeda salsa L. is a leaf succulent euhalophyte. Shoots of the S. salsa are larger and more succulent when grown in highly saline environments. This increased growth and water uptake has been correlated with a large and specific cellular accumulation of sodium. S. salsa does not have salt glands or salt bladders on its leaves. Thus, this plant must compartmentalize the toxic Na(+) in the vacuoles. The ability to compartmentalize sodium may result from a stimulation of the proton pumps that provide the driving force for increased sodium transport into the vacuole. In this work, we isolated the cDNA of the vacuolar membrane proton-translocating inorganic pyrophosphatase (H(+) -PPase) from S. salsa. The SsVP cDNA contains an uninterrupted open reading frame of 2292 bp, coding for a polypeptide of 764 amino acids. Northern blotting analysis showed that SsVP was induced in salinity treated leaves. The activities of both the V-ATPase and the V-PPase in Arabidopsis overexpressing SsVP-2 is higher markedly than in wild-type plant under 200 mM NaCl and drought stresses. The Overexpression of SsVP can increase salt and drought tolerance of transgenic Arabidopsis.
Guo
Shanli
S
Key Laboratory of Plant Stress Research, Life Science College, Shandong Normal University, 250014, Jinan, China.
Yin
Haibo
H
Zhang
Xia
X
Zhao
Fengyun
F
Li
Pinghua
P
Chen
Shihua
S
Zhao
Yanxiu
Y
Zhang
Hui
H
D000222:Adaptation, Physiological / Q000187:drug effects; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D019663:Chenopodiaceae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular; D003890:Desiccation; D015870:Gene Expression; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D011755:Pyrophosphatases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D014867:Water / Q000494:pharmacology*
D014867:Water; D012965:Sodium Chloride; D011755:Pyrophosphatases