Analyzing the dynamic bacterial glycome with a lectin microarray approach.
Glycosylation of bacterial cell surfaces is emerging as a critical factor in symbiosis, pathogenesis, cell-cell interactions and immune evasion. The lack of high-throughput analytical tools to examine bacterial glycans has been a major obstacle to the field and has hindered closer examination of the dynamics of carbohydrate variation. We have recently developed a lectin microarray for the analysis of glycoproteins. Herein we present a rapid analytical system based on this technology for the examination of bacterial glycans. The glycosylation pattern observed distinguishes closely related Escherichia coli strains from one another, providing a facile means of fingerprinting bacteria. In addition, dynamic alterations in the carbohydrate coat of a pathogenic E. coli strain are readily observed. The fast evaluation of real-time alterations in surface-carbohydrate epitopes allows examination of the dynamic role of bacterial sugars in response to external stimuli such as the immune system.
Hsu
Ku-Lung
KL
Department of Chemistry and Biochemistry, Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, 1 University Station, A5300, Austin, Texas 78712-0265, USA.
Pilobello
Kanoelani T
KT
Mahal
Lara K
LK
D001419:Bacteria / Q000737:chemistry* / Q000145:classification*; D001431:Bacteriological Techniques / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis*; D040081:Protein Array Analysis / Q000379:methods*; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D037102:Lectins; D011134:Polysaccharides