title: Change of potassium current density in rabbit corneal epithelial cells during maturation and cellular senescence.
abstract: BACKGROUND
  Voltage-gated potassium (K+) channels may participate in cellular developmental regulation, including cell differentiation, proliferation and apoptosis. This study investigated the change of K + current densities in corneal epithelial cells during maturation and cellular senescence.
  
  
  METHODS
  New Zealand white rabbits were divided into three age groups: newborn (<or= 7 days old, n = 18); young (8-12 weeks old, n = 59); and adult (20-28 weeks old, n = 16). Rabbit corneal epithelial cells were subdivided into the following three groups: small cells with capacitance < 6.0 pF; medium cells with capacitance 6.0-10.0 pF; and large cells with capacitance > 10.0 pF. Using a whole-cell clamp technique, K+ current was recorded and current densities were calculated. Differences in K+ current densities among newborn, young and adult rabbits, as well as differences among small, medium and large cells, were analyzed.
  
  
  RESULTS
  We delineated two types of cells manifesting different amplitudes of depolarization-activated K+ outward currents. The averaged current density of type 1 response cells was significantly larger than that of type 2 cells in newborn, young, and adult groups. For newborn epithelial cells, the depolarization-gated outward K+ current density decreased from small to medium to large cells (p = 0.049, at a membrane potential of 140 mV). A similar pattern of change in current density was also delineated for these cell sizes in young and adult rabbit corneal cells (p < 0.001 for both young and adult rabbits). An increase in depolarization-gated outward K+ current density was also delineated from newborn to young to adult rabbits (p < 0.001, p < 0.001 and p < 0.006 for small, medium and large cells, respectively, at a membrane potential of 140 mV).
  
  
  CONCLUSIONS
  Corneal epithelial cells expressed K+ channel densities that were distinct from basal to superficial cells and from newborn to adult rabbits.
authors.affiliation: Department of Ophthalmology, Far Eastern Memorial Hospital, Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan, R.O.C.
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Chang
authors.forename: Gwo-Jyh
authors.initials: GJ
authors.lastname: Chang
authors.forename: Ming-Jai
authors.initials: MJ
authors.lastname: Su
mesh_terms: D000818:Animals; D016922:Cellular Senescence / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism*; D019573:Epithelium, Corneal / Q000166:cytology / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism*; D011817:Rabbits
chemical_list: D024642:Potassium Channels, Voltage-Gated