Tips for extracting total RNA from chondrocytes cultured in agarose gel using a silica-based membrane kit.
Mio
Kensuke
K
Academic Unit of Musculo-Skeletal and Rehabilitation Medicine, Bioengineering Division, University of Leeds, Leeds LS2 9NZ, UK. ken-mio@muh.biglobe.ne.jp
Kirkham
Jennifer
J
Bonass
William A
WA
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D019902:Chondrocytes / Q000737:chemistry*; D003470:Culture Media; D006146:Guanidines; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D012313:RNA / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012685:Sepharose; D012822:Silicon Dioxide / Q000737:chemistry; D013861:Thiocyanates
D003470:Culture Media; D006146:Guanidines; D008567:Membranes, Artificial; D011933:Reagent Kits, Diagnostic; D013861:Thiocyanates; C054436:guanidine thiocyanate; D012313:RNA; D012822:Silicon Dioxide; D012685:Sepharose