Effect of resveratrol on pancreatic oxygen free radicals in rats with severe acute pancreatitis.
OBJECTIVE
To investigate the therapeutic effects of resveratrol (RESV) as a free radical scavenger on experimental severe acute pancreatitis (SAP).


METHODS
Seventy-two male Sprague-Dawley rats were divided randomly into sham operation group, SAP group, and resveratrol-treated group. Pancreatitis was induced by intraductal administration of 0.1 mL/kg 4% sodium taurocholate. RESV was given intravenously at a dose of 20 mg/kg body weight. All animals were killed at 3, 6, 12 h after induction of the model. Serum amylase, pancreatic superoxide dismutase (SOD), malondialdehyde (MDA), and myeloperoxidase (MPO) were determined. Pathologic changes of the pancreas were observed under optical microscope.


RESULTS
The serum amylase, pancreatic MPO and the score of pathologic damage increased after the induction of pancreatitis, early (3, 6 h) SAP samples were characterized by decreased pancreatic SOD and increased pancreatic MDA. Resveratrol exhibited a protective effect against lipid peroxidation in cell membrane caused by oxygen free radicals in the early stage of SAP. This attenuation of the redox state impairment reduced cellular oxidative damage, as reflected by lower serum amylase, less severe pancreatic lesions, normal pancreatic MDA levels, as well as diminished neutrophil infiltration in pancreas.


CONCLUSIONS
RESV may exert its therapeutic effect on SAP by lowering pancreatic oxidative free radicals and reducing pancreatic tissue infiltration of neutrophils.
Li
Zhen-Dong
ZD
Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University, 1 Jiankang Road, Xi'an 710061, Shaanxi Province China. victor7922@163.com
Ma
Qing-Yong
QY
Wang
Chang-An
CA
D000208:Acute Disease; D000818:Animals; D005609:Free Radicals; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009504:Neutrophils / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism*; D010195:Pancreatitis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes; D013482:Superoxide Dismutase / Q000378:metabolism
D005609:Free Radicals; D013267:Stilbenes; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase; D000077185:Resveratrol; D010100:Oxygen