title: Chromosomal differentiation of populations of Lysapsus limellus limellus, L. l. bolivianus, and of Lysapsus caraya (Hylinae, Hylidae).
abstract: Cytogenetic analysis were done on specimens from two populations of Lysapsus limellus limellus, three of L. l. bolivianus and of one of Lysapsus caraya. All animals showed a diploid chromosomal number of 2n=24. The karyotypes of the two L. limellus subspecies were very similar, differing only by the larger amount of telomeric heterochromatin and a small pericentromeric C-band on the short arms of pair 2 in L. l. limellus specimens. The karyotype of L. caraya differed from those of the two L. limellus subspecies in terms of chromosomal morphology, C-banding pattern and location of the main NOR on chromosomes 7 and 6, respectively. The karyotype of the L. l. bolivianus population from Guajará-Mirim/RO differed from those of the other populations of the same subspecies in morphology and heterochromatin pattern of chromosomes 7 and 8. Additional NORs were detected by silver staining and confirmed by FISH in one of the homologues of pairs 1 and 8 in L. l. bolivianus and in pair 7 in L. caraya. These results suggest that a reassessment of the taxonomic status of L. limellus subspecies, especially of the L. l. bolivianus populations, may be necessary.
authors.affiliation: Departamento de Biologia Celular, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-863 Campinas, SP, Brazil.
authors.forename: Carmen Sílvia
authors.initials: CS
authors.lastname: Busin
authors.forename: Albertina Pimentel
authors.initials: AP
authors.lastname: Lima
authors.forename: Cynthia Peralta
authors.initials: CP
authors.lastname: de Almeida Prado
authors.forename: Christine
authors.initials: C
authors.lastname: Strüssmann
authors.forename: Sérgio Siqueira
authors.initials: SS
authors.lastname: Júnior
authors.forename: Shirlei Maria
authors.initials: SM
authors.lastname: Recco-Pimentel
mesh_terms: D000818:Animals; D001001:Anura / Q000145:classification* / Q000235:genetics*; D002875:Chromosomes / Q000648:ultrastructure*; D003582:Cytogenetics; D004171:Diploidy; D005260:Female; D006570:Heterochromatin / Q000648:ultrastructure; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D012834:Silver; D013045:Species Specificity; D013194:Staining and Labeling
chemical_list: D006570:Heterochromatin; D012834:Silver