title: Identification and characterization of a novel Mt-retrotransposon highly represented in the female mouse germline.
abstract: The control of primordial follicle recruitment into the growing follicle population is a major limiting process in female reproduction. In order to gain insight into the molecular processes occurring at the time of primordial follicle activation, a subtractive hybridization analysis was performed between cDNAs prepared from temporally distinct mouse neonatal ovarian tissues that differed according to the state of primordial follicle activation. One highly represented clone associated with activation was an Mt retrotransposon-like sequence designated Mtfull, which was subsequently cloned and determined to be novel and restricted in expression to the ovary. The polyadenylated 1684-bp sequence has long terminal repeats, is predicted to be noncoding, and is the predominant Mti-related sequence present in the mouse ovary. In situ hybridization further localized Mtfull expression to the oocyte and confirmed that expression is concomitant with follicle activation. Together with in silico data, we predict Mtfull plays an essential role in folliculogenesis through regulation of gene expression.
authors.affiliation: Reproductive Science Group, Discipline of Biological Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia. janet.holt@studentmail.newcastle.edu.au
authors.forename: Janet E
authors.initials: JE
authors.lastname: Holt
authors.forename: Shaun D
authors.initials: SD
authors.lastname: Roman
authors.forename: R John
authors.initials: RJ
authors.lastname: Aitken
authors.forename: Eileen A
authors.initials: EA
authors.lastname: McLaughlin
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary; D016208:Databases, Factual; D020224:Expressed Sequence Tags; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D006080:Ovarian Follicle / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D018626:Retroelements / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D020079:Terminal Repeat Sequences; D024363:Transcription Initiation Site; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D018076:DNA, Complementary; D018626:Retroelements