Cell cycle and radiosensitivity of progeny of irradiated primary cultured human hepatocarcinoma cells.
OBJECTIVE
To evaluate the change of growth characteristics and radiosensitivity of irradiated primary cultured human hepatocarcinoma cells.


METHODS
All tumor tissue samples were obtained from 39 hepatocarcinoma patients with a mean age of 49.6 years (range 22-76 years). We divided the samples into irradiated group and non-irradiated group and measured their plating efficiency (PE), population doubling time (PDT), radiosensitivity index SF2 and cell cycle.


RESULTS
The PDT of primary culture of hepatocarcinoma cells was 91.0+/-6.6 h, PE was 12.0+/-1.4%, SF2 was 0.41+/-0.05%. The PDT of their irradiated progeny was 124.8+/-5.8 h, PE was 5.0+/-0.7%, SF2 was 0.65+/-0.09%. The primary cultured human hepatocarcinoma cells showed significant S reduction and G(2) arrest in a dose-dependent manner. The progeny of irradiated primary cultured hepatocarcinoma cells grew more slowly and its radiosensitivity increased.


CONCLUSIONS
The progeny of irradiated primary cultured human hepatocarcinoma cells grows more slowly and its radiosensitivity increases.
Liu
Zhi-Zhong
ZZ
Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. whzzl@163.net
Huang
Wen-Ying
WY
Lin
Ju-Sheng
JS
Li
Xiao-Sheng
XS
Liang
Kuo-Huan
KH
Huang
Jia-Long
JL
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular; D002453:Cell Cycle / Q000502:physiology*; D002470:Cell Survival; D006801:Humans; D008113:Liver Neoplasms; D008875:Middle Aged; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D014965:X-Rays