title: Characterization of an Indonesian isolate of Paecilomyces reniformis.
abstract: An entomopathogenic fungus (IndGH 96), identified as Paecilomyces reniformis, was isolated from long-horned grasshoppers (Orthoptera: Tettigoniidae) in Sulawesi, Indonesia. The phenotypic and molecular data identified the IndGH 96 as a P. reniformis. We present the first comprehensive characterization of this species using morphological features, sequencing of the ITS1-5.8s-ITS2 region, D1/D2 region of 28S of rDNA, and a portion of the tubulin gene, and laboratory bioassays. Distinguishing features include a hyphal body stage during vegetative growth and the production of distinctly curved, light-green conidia. High dosage bioassays showed that IndGH 96 was infectious to both long-horned and short-horned grasshoppers but not to the house cricket, Acheta domestica, or to the lepidopterans velvetbean caterpillar, Anticarsia gemmatalis or fall armyworm, Spodoptera frugiperda. Phenotypic and genetic analyses suggest that IndGH 96 and other isolates of P. reniformis are more closely related to Nomuraea rileyi than to other species of Paecilomyces.
authors.affiliation: Department of Biology, Kahramanmaras Sütçü Imam University, Kahramanmaraş, Turkey. ozlemk@ksu.edu.tr
authors.forename: O
authors.initials: O
authors.lastname: Kalkar
authors.forename: G R
authors.initials: GR
authors.lastname: Carner
authors.forename: D
authors.initials: D
authors.lastname: Scharf
authors.forename: D G
authors.initials: DG
authors.lastname: Boucias
mesh_terms: D000818:Animals; D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D006110:Grasshoppers / Q000382:microbiology*; D007214:Indonesia; D008855:Microscopy, Electron, Scanning; D010143:Paecilomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012339:RNA, Ribosomal, 28S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D014404:Tubulin / Q000737:chemistry / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012339:RNA, Ribosomal, 28S; D014404:Tubulin