title: Expression of parathyroid hormone-related protein (PTHrP) mRNA in mammary gland of periparturient cows.
abstract: The expression of parathyroid hormone-related protein (PTHrP) mRNA was examined in mammary gland with or without lactation, and during periparturient period in a Holstein cow and a Jersey cow. In the lactating mammary gland, PTHrP was detected in alveolar epithelial cells and the lumen by immunohistochemical analysis. The relative expression levels of PTHrP mRNA in mammary gland from lactating cows were significantly higher than those from non-lactating cows (P<0.05). During periparturient period, relative PTHrP mRNA level was remarkably low before the parturition in a Jersey and a Holstein cow, however, both levels were gradually increased and reached a peak level at 5-6 weeks after the parturition. In addition, the peak level in a Jersey cow was approximately 3-fold higher than that in a Holstein cow. From these results, PTHrP was synthesized and secreted in alveolar epithelial cells in mammary gland and increased subsequently with the lactation, suggesting a possible mechanism for the regulation of local calcium homeostasis.
authors.affiliation: Laboratory of Internal Medicine III, Azabu University School of Veterinary Medicine, 1-17-71 Fuchinobe, Kanagawa 229-8501, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Onda
authors.forename: Naoaki
authors.initials: N
authors.lastname: Matsuki
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Ono
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Wada
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle / Q000378:metabolism*; D017931:DNA Primers; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006706:Homeostasis / Q000502:physiology; D007150:Immunohistochemistry; D007774:Lactation / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D044162:Parathyroid Hormone-Related Protein / Q000378:metabolism*; D036801:Parturition / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D044162:Parathyroid Hormone-Related Protein; D012333:RNA, Messenger; D002118:Calcium