title: Delineating signal transduction pathways in smooth muscle through focused proteomics.
abstract: This review will outline examples of the authors' focused proteomics approaches to studying signal transduction pathways in smooth muscle. By focusing the use of traditional proteomics techniques with hypothesis-driven selection methods, this approach efficiently addresses the identification of novel elements in a signal transduction pathway of interest. However, focused proteomics serves only as a starting point in the investigation of novel signaling proteins. While focused proteomics studies can suggest the involvement and general biochemical function of a protein in a signaling pathway, these findings must be further investigated and validated. Through the integrated use of focused proteomics with complementary approaches such as genetics, biochemistry and cell physiology, a complete and detailed mechanism of signal transduction can be determined.
authors.affiliation: Department of Pharmacology & Cancer Biology, Duke University, C118 LSRC, Durham, NC 27710, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Hagerty
authors.forename: Timothy A J
authors.initials: TA
authors.lastname: Haystead
mesh_terms: D000818:Animals; D013058:Mass Spectrometry; D009130:Muscle, Smooth / Q000378:metabolism*; D010766:Phosphorylation; D020543:Proteome / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics; D015398:Signal Transduction
chemical_list: D020543:Proteome