title: A two-center international evaluation of the Immulite 2000 automated serum gastrin assay.
abstract: OBJECTIVE
  To assess the performance of the Immulite 2000 serum gastrin assay.
  
  
  METHODS
  Two-center validation and comparison with two manual gastrin assays.
  
  
  RESULTS
  Serum, serum separator, EDTA and heparin tube results differed <20%. Samples showed <20% degradation for <6 h at room temperature, 3-day-refrigerated, and underwent 3 freeze-thaw cycles. Imprecision was <8% between 20 and 824 ng/L (10-393 pmol/L). Spike recovery was 88.5-106%, and recovery on dilution was 62-135%. There were no interferences from gastrin 1-14, pentagastrin, and cholecystokinin (CCK) 1-33 and CCK 26-33. The fasting reference range was <100 ng/L (<48 pmol/L). Regression slopes to the manual assays were 0.9 and 0.97, with comparable clinical performance.
  
  
  CONCLUSIONS
  The Immulite 2000 assay is precise, accurate, and fast and compares well with established gastrin assays.
authors.affiliation: Endocrine Laboratory, Department of Laboratory Medicine and Pathology, Hilton 730C, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USA.
authors.forename: Mary L
authors.initials: ML
authors.lastname: Eastvold
authors.forename: Hans W
authors.initials: HW
authors.lastname: Wallinder
authors.forename: Carol M
authors.initials: CM
authors.lastname: Preissner
authors.forename: Ravinder J
authors.initials: RJ
authors.lastname: Singh
authors.forename: Christian
authors.initials: C
authors.lastname: Löwbeer
authors.forename: Stefan K G
authors.initials: SK
authors.lastname: Grebe
mesh_terms: D001331:Automation; D003429:Cross Reactions; D005755:Gastrins / Q000097:blood*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D005755:Gastrins