Bioconjugation of Ln3+-doped LaF3 nanoparticles to avidin.
The binding of Eu3+-doped LaF3 nanoparticles with biotin moieties at the surface of the stabilizing ligand layer to avidin, immobilized on cross-linked aragose beads, is described. The biotin moieties were attached to the nanoparticles by reaction of an activated ester with the amino groups on the surface of the nanoparticles resulting from the 2-aminoethyl phosphate ligands that were coordinated to the surface through the phosphate end. This strategy of employing the reactions of amines with activated esters provides a general platform to modify the surface of the 2-aminophosphate stabilized Ln3+-doped LaF3 nanoparticles with biologically relevant groups. Significant suppression of nonspecific binding to the avidin modified aragose beads has been realized by the incorporation of poly(ethylene glycol) units via the same reaction of a primary amine with an activated ester. The particle size distribution of the functionalized nanoparticles was within 10-50 nm, with a quantum yield of 19% in H2O for the LaF3 nanoparticles codoped with Ce3+ and Tb3+. A discreet, 4 unit poly(ethylene glycol) spaced heterobifunctional cross-linker, functionalized with biotin and N-hydroxysuccinimide at opposite termini, was covalently linked to the 2-aminoethyl phosphate ligand via the N-hydroxysuccinimide activated ester, making an amide bond, imparting biological activity to the particle. Modification of the remaining unreacted amino groups of the stabilizing ligands was done with Me(OCH2CH2)3CH2CH2(C=O)-NHS (NHS = N-hydroxysuccinimide).
Diamente
Peter R
PR
University of Victoria, Department of Chemistry, P.O. Box 3065, Victoria, British Columbia, Canada V8W 3V6.
Burke
Robert D
RD
van Veggel
Frank C J M
FC
D001360:Avidin / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry*; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D007811:Lanthanum / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size
D028581:Lanthanoid Series Elements; C083668:lanthanum trifluoride; D001360:Avidin; D007811:Lanthanum; D005459:Fluorides