Single-stranded DNA of Tomato leaf curl virus accumulates in the cytoplasm of phloem cells.
Geminiviruses have been reported to replicate in, and localize to, the nuclei of host plant cells. We have investigated the tissue and intracellular distribution of the monopartite Tomato leaf curl virus (TLCV) by in situ hybridization. Contrary to the current understanding of geminiviral localization, single-stranded (ss) DNA of TLCV accumulated in the cytoplasm. TLCV ssDNA was also found in the nucleus, as was lower levels of replicative form double-stranded (ds) DNA. Under the same conditions, Tomato golden mosaic virus (TGMV) ssDNA and dsDNA were found in nuclei. ssDNA of TLCV, TGMV, and Tomato yellow leaf curl Sardinia virus (TYLCSV) was detected in some xylem vessels under specific hybridization conditions. Tissue specificity of TLCV was partially released by co-infection with TGMV. Our observations suggest that the mechanism of TLCV movement may differ from that of bipartite begomoviruses.
Rasheed
M Saif
MS
CSIRO Plant Industry, P.O. Box 350, Glen Osmond, SA 5064, Australia.
Selth
Luke A
LA
Koltunow
Anna M G
AM
Randles
John W
JW
Rezaian
M Ali
MA
D002467:Cell Nucleus / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry* / Q000821:virology*; D004247:DNA / Q000032:analysis / Q000235:genetics; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D017791:Geminiviridae / Q000235:genetics / Q000502:physiology*; D017403:In Situ Hybridization; D018551:Solanum lycopersicum / Q000821:virology; D008857:Microscopy, Interference; D010784:Photomicrography; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D018517:Plant Roots / Q000821:virology; D018547:Plant Stems / Q000821:virology; D014026:Nicotiana / Q000821:virology
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004247:DNA