Identification of differentially expressed genes in brown planthopper Nilaparvata lugens (Hemiptera: Delphacidae) responding to host plant resistance.
The brown planthopper Nilaparvata lugens Stål is one of the major insect pests of rice Oryza sativa L. The host resistance exhibits profound effects on growth, development and propagation of N. lugens. To investigate the molecular response of N. lugens to host resistance, a cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique was employed to identify the differentially expressed genes in the nymphs feeding on three rice varieties. Of the 2,800 cDNA bands analysed, 54 were up-regulated and seven down-regulated qualitatively in N. lugens when the ingestion sources were changed from susceptible rice plants to resistant ones. Sequence analysis of the differential transcript-derived fragments showed that the genes involved in signalling, stress response, gene expression regulation, detoxification and metabolism were regulated by host resistance. Four of the transcript-derived fragments corresponding to genes encoding for a putative B subunit of phosphatase PP2A, a nemo kinase, a cytochrome P450 monooxygenase and a prolyl endopeptidase were further characterized in detail. Northern blot analysis confirmed that the expression of the four genes was enhanced in N. lugens feeding on resistant rice plants. The roles of these genes in the defensive response of N. lugens to host plant resistance were discussed.
Yang
Zhifan
Z
Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Zhang
Futie
F
Zhu
Lili
L
He
Guangcun
G
D000818:Animals; D001483:Base Sequence / Q000235:genetics; D015152:Blotting, Northern / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000737:chemistry; D015536:Down-Regulation / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D017344:Genes, Insect / Q000235:genetics*; D006430:Hemiptera / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009758:Nymph / Q000235:genetics / Q000502:physiology; D012275:Oryza / Q000276:immunology / Q000469:parasitology / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics; D017385:Sequence Homology; D015854:Up-Regulation / Q000235:genetics
D018076:DNA, Complementary; D012313:RNA