title: Biocompatibility of Beta-tricalcium phosphate root replicas in porcine tooth extraction sockets - a correlative histological, ultrastructural, and x-ray microanalytical pilot study.
abstract: This investigation studies porcine tissue response in tooth extraction sockets treated with root replicas made out of Beta-tricalcium phosphate (Beta-TCP; Beta-Ca(3)(PO(4))(2)) granules, molded and held together by thermal fusion of a thin film of polyglycolic-polylactic acid copolymer. Six left mandibular third incisors (n (1)/4 6) of experimental pigs are treated with the root replicas and four contralateral incisors are used as nontreated controls (n (1)/4 4). Two animals each were killed at 20, 40, and 60 weeks of observation periods. The mandibular jaw segments were prepared in toto for light microscopy by resin embedding and serial ground sectioning. Additionally, one Beta-TCP-treated socket at 60 weeks was thoroughly investigated by correlative light, electron microscopic and electron probe X-ray microanalysis to assess the bio-absorbability and host removal of the replica material from the implant site. The extraction wounds of the animals healed satisfactorily with very little histologically observable differences in the healing pattern of the test and control sites. The Beta-TCP was completely removed from extracellular sites, but at 60 weeks, remnants of it were found in the cytoplasm of multinucleated giant cells. The root replicas made out of Beta-TCP were biocompatible and bioabsorbable. Osseous healing occurred both in the test and control sockets, but the healing process was delayed due to the presence of Beta-TCP particles.
authors.affiliation: Institute of Oral Biology, Section for Oral Structures and Development, Centre of Dental and Oral Medicine, University of Zurich, Zurich, Switzerland. nair@zzmk.unizh.ch
authors.forename: P N Ramachandran
authors.initials: PN
authors.lastname: Nair
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Luder
authors.forename: Fabrizio A
authors.initials: FA
authors.lastname: Maspero
authors.forename: Jürgen H
authors.initials: JH
authors.lastname: Fischer
authors.forename: Jens
authors.initials: J
authors.lastname: Schug
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018786:Bone Substitutes / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D003758:Dental Implantation, Endosseous / Q000295:instrumentation / Q000379:methods; D019250:Dental Implants, Single-Tooth; D019544:Equipment Failure Analysis; D048091:Guided Tissue Regeneration / Q000295:instrumentation / Q000379:methods; D007180:Incisor / Q000166:cytology* / Q000502:physiology / Q000601:surgery*; D008422:Materials Testing; D016348:Osseointegration; D013223:Statistics as Topic; D013552:Swine; D014081:Tooth Extraction; D014092:Tooth Root / Q000166:cytology* / Q000502:physiology / Q000601:surgery*
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate