title: MurQ Etherase is required by Escherichia coli in order to metabolize anhydro-N-acetylmuramic acid obtained either from the environment or from its own cell wall.
abstract: MurQ is an N-acetylmuramic acid-phosphate (MurNAc-P) etherase that converts MurNAc-P to N-acetylglucosamine-phosphate and is essential for growth on MurNAc as the sole source of carbon (T. Jaegar, M. Arsic, and C. Mayer, J. Biol. Chem. 280:30100-30106, 2005). Here we show that MurQ is the only MurNAc-P etherase in Escherichia coli and that MurQ and AnmK kinase are required for utilization of anhydro-MurNAc derived either from cell wall murein or imported from the medium.
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA. tsuyoshi.uehara@tufts.edu
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Uehara
authors.forename: Kyoko
authors.initials: K
authors.lastname: Suefuji
authors.forename: Tina
authors.initials: T
authors.lastname: Jaeger
authors.forename: Christoph
authors.initials: C
authors.lastname: Mayer
authors.forename: James T
authors.initials: JT
authors.lastname: Park
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D009112:Muramic Acids / Q000378:metabolism*
chemical_list: D003470:Culture Media; D029968:Escherichia coli Proteins; D009112:Muramic Acids; C430327:anhydro-N-acetylmuramic acid; D006026:Glycoside Hydrolases; C504247:MurQ protein, E coli