title: Analysis of the mechanism of protection in transgenic plants expressing the potato virus X coat protein or its antisense RNA.
abstract: Transgenic tobacco plants engineered to express either the potato virus X (PVX) coat protein (CP+) or the antisense coat protein transcript (CP-antisense) were protected from infection by PVX, as indicated by reduced lesion numbers on inoculated leaves, delay or absence of systemic symptom development and reduction in virus accumulation in both inoculated and systemic leaves. The extent of protection observed in CP+ plants primarily depended upon the level of expression of the coat protein. Plants expressing antisense RNA were protected only at low inoculum concentrations. The extent of this protection was even lower than that observed in plants expressing low levels of CP. In contrast to previous reports for plants expressing tobacco mosaic virus or alfalfa mosaic virus CP, inoculation of plants expressing high levels of PVX CP with PVX RNA did not overcome the protection. Specifically, lesion numbers on inoculated leaves and PVX levels on inoculated and systemtic leaves of the CP+ plants were reduced to a similar extent in both virus and RNA inoculated plants. Although these results do not rule out that CP-mediated protection involves inhibition of uncoating of the challenge virus, they suggest that PVX CP (or its RNA) can moderate early events in RNA infection by a different mechanism.
authors.affiliation: Plant Molecular Biology, Division of Biological Sciences, Monsanto Company, 700 Chesterfield Village Parkway, St Louis, MO 63198, USA.
authors.forename: C
authors.initials: C
authors.lastname: Hemenway
authors.forename: R X
authors.initials: RX
authors.lastname: Fang
authors.forename: W K
authors.initials: WK
authors.lastname: Kaniewski
authors.forename: N H
authors.initials: NH
authors.lastname: Chua
authors.forename: N E
authors.initials: NE
authors.lastname: Tumer