Identification and comparative analysis of the large subunit mitochondrial ribosomal proteins of Neurospora crassa.
The mitochondrial ribosome (mitoribosome) has highly evolved from its putative prokaryotic ancestor and varies considerably from one organism to another. To gain further insights into its structural and evolutionary characteristics, we have purified and identified individual mitochondrial ribosomal proteins of Neurospora crassa by mass spectrometry and compared them with those of the budding yeast Saccharomyces cerevisiae. Most of the mitochondrial ribosomal proteins of the two fungi are well conserved with each other, although the degree of conservation varies to a large extent. One of the N. crassa mitochondrial ribosomal proteins was found to be homologous to yeast Mhr1p that is involved in homologous DNA recombination and genome maintenance in yeast mitochondria.
Gan
Xiang
X
Department of Biology, Faculty of Science, Kobe University, Kobe, Japan.
Arita
Kyoko
K
Isono
Setsuko
S
Kitakawa
Madoka
M
Yoshino
Ken-ichi
K
Yonezawa
Kazuyoshi
K
Kato
Akihiro
A
Inoue
Hirokazu
H
Isono
Katsumi
K
D006801:Humans; D013058:Mass Spectrometry; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure*; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
C417727:MHR1 protein, S cerevisiae; D009687:Nuclear Proteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors