Regulation of genes involved in dopamine transporter modulation by acute cocaine in rat striatum.
It is well established that acute administration of psychostimulants alters dopamine transport. However, the exact mechanism of this modulation is still unknown. In this study we examined the mRNA levels of several proteins involved in the various proposed processes following cocaine administration. The expression levels of several immediate early genes were also studied. This was performed in rat striatum using real-time quantitative PCR. As expected, a marked increase of the immediate early genes Fos, Egr1 and Egr3 was observed. Egr2 was also found up-regulated. Among the different genes studied only Synaptotagmin4 in the SNARE family and Synphilin1 in the synaptic vesicles binding family were modulated by acute cocaine treatment. Interestingly, acute amphetamine treatment did not increase either Synaptotagmin4 and Synphilin1 mRNA levels, although increases in early genes expression were noted.
Courtin
Cindie
C
CNRS UMR 7157, Paris F-75006, France.
Crete
Dominique
D
Canestrelli
Corinne
C
Noble
Florence
F
Marie-Claire
Cynthia
C
D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D002966:Clathrin / Q000502:physiology; D003042:Cocaine / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D004705:Endocytosis; D017781:Genes, Immediate-Early; D008297:Male; D011485:Protein Binding; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013572:Synaptic Vesicles / Q000378:metabolism
D000697:Central Nervous System Stimulants; D002966:Clathrin; D050483:Dopamine Plasma Membrane Transport Proteins; D012333:RNA, Messenger; D000661:Amphetamine; D003042:Cocaine