title: Design and use of conditional MHC class I ligands.
abstract: Major histocompatibility complex (MHC) class I molecules associate with a variety of peptide ligands during biosynthesis and present these ligands on the cell surface for recognition by cytotoxic T cells. We have designed conditional MHC ligands that form stable complexes with MHC molecules but degrade on command, by exposure to a defined photostimulus. 'Empty MHC molecules' generated in this manner can be loaded with arrays of peptide ligands to determine MHC binding properties and to monitor antigen-specific T-cell responses in a high-throughput manner. We document the value of this approach by identifying cytotoxic T-cell epitopes within the H5N1 influenza A/Vietnam/1194/04 genome.
authors.affiliation: Division of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
authors.forename: Mireille
authors.initials: M
authors.lastname: Toebes
authors.forename: Miriam
authors.initials: M
authors.lastname: Coccoris
authors.forename: Adriaan
authors.initials: A
authors.lastname: Bins
authors.forename: Boris
authors.initials: B
authors.lastname: Rodenko
authors.forename: Raquel
authors.initials: R
authors.lastname: Gomez
authors.forename: Nella J
authors.initials: NJ
authors.lastname: Nieuwkoop
authors.forename: Willeke
authors.initials: W
authors.lastname: van de Kasteele
authors.forename: Guus F
authors.initials: GF
authors.lastname: Rimmelzwaan
authors.forename: John B A G
authors.initials: JB
authors.lastname: Haanen
authors.forename: Huib
authors.initials: H
authors.lastname: Ovaa
authors.forename: Ton N M
authors.initials: TN
authors.lastname: Schumacher
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015195:Drug Design; D015789:HLA-A2 Antigen / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000235:genetics; D066298:In Vitro Techniques; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology; D008024:Ligands; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008958:Models, Molecular; D015394:Molecular Structure; D046912:Multiprotein Complexes; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D010777:Photochemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014466:Ultraviolet Rays
chemical_list: D015789:HLA-A2 Antigen; D015395:Histocompatibility Antigens Class I; D016056:Immunodominant Epitopes; D008024:Ligands; D046912:Multiprotein Complexes; D009842:Oligopeptides