title: Freezing induces biased results in the molecular detection of Flavobacterium columnare.
abstract: Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR. The detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish.
authors.affiliation: Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35, FIN-40014 University of Jyväskylä, Finland.
authors.forename: Lotta-Riina
authors.initials: LR
authors.lastname: Suomalainen
authors.forename: Hilkka
authors.initials: H
authors.lastname: Reunanen
authors.forename: Ritva
authors.initials: R
authors.lastname: Ijäs
authors.forename: E Tellervo
authors.initials: ET
authors.lastname: Valtonen
authors.forename: Marja
authors.initials: M
authors.lastname: Tiirola
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005188:False Negative Reactions; D005417:Flavobacterium / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D005615:Freezing; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial