Etiologic diagnosis of adult bacterial pneumonia by culture and PCR applied to respiratory tract samples.
Respiratory culture and multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, and Chlamydophila pneumoniae were applied to sputum, nasopharyngeal swabs, and nasopharyngeal aspirates from 235 adult patients with community-acquired pneumonia and 113 controls. Both culture and multiplex PCR performed well with the different samples and appear to be useful as diagnostic tools.
Strålin
Kristoffer
K
Department of Infectious Diseases, Orebro University Hospital, SE-70185 Orebro, Sweden. kristoffer.stralin@orebroll.se
Törnqvist
Eva
E
Kaltoft
Margit Staum
MS
Olcén
Per
P
Holmberg
Hans
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques; D016993:Chlamydophila pneumoniae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017714:Community-Acquired Infections / Q000175:diagnosis / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D009177:Mycoplasma pneumoniae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D018410:Pneumonia, Bacterial / Q000175:diagnosis* / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012137:Respiratory System / Q000382:microbiology; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
D003470:Culture Media; D004269:DNA, Bacterial