title: Engineered biosynthesis of plant polyketides: manipulation of chalcone synthase.
abstract: [reaction: see text]. Chalcone synthase (CHS) is a plant-specific type III polyketide synthase catalyzing condensation of 4-coumaroyl-CoA with three molecules of malonyl-CoA. Surprisingly, it was demonstrated that S338V mutant of Scutellaria baicalensis CHS produced octaketides SEK4/SEK4b from eight molecules of malonyl-CoA. Further, the octaketides-forming activity was dramatically increased in a CHS triple mutant (T197G/G256L/S338T). The functional conversion is based on the simple steric modulation of a chemically inert residue lining the active-site cavity.
authors.affiliation: School of Pharmaceutical Sciences and the COE 21 Program, University of Shizuoka, Shizuoka 422-8526, Japan. abei@ys7.u-shizuoka-ken.ac.jp
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Watanabe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kohno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Noguchi
mesh_terms: D000217:Acyltransferases / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites / Q000502:physiology; D018942:Macrolides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D008968:Molecular Conformation; D015394:Molecular Structure; D010944:Plants / Q000737:chemistry* / Q000201:enzymology*; D014176:Protein Biosynthesis; D031364:Scutellaria baicalensis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D018942:Macrolides; D000217:Acyltransferases; C020141:flavanone synthetase