title: Chromatin compaction at the mononucleosome level.
abstract: Using a previously described FRET technique, we measured the distance between the ends of DNA fragments on which nucleosomes were reconstituted from recombinant and native histones. This distance was analyzed in its dependence on the DNA fragment length, concentration of mono- and divalent counterions, presence of linker histone H1, and histone modifications. We found that the linker DNA arms do not cross under all conditions studied but diverge slightly as they leave the histone core surface. Histone H1 leads to a global approach of the linker DNA arms, confirming the notion of a "stem structure". Increasing salt concentration also leads to an approach of the linker DNAs. To study the effect of acetylation, we compared chemically acetylated recombinant histones with histones prepared from HeLa cells, characterizing the sites of acetylation by mass spectroscopy. Nucleosomes from chemically acetylated histones have few modifications in the core domain and form nucleosomes normally. Acetylating all histones or selectively only H3 causes an opening of the nucleosome structure, indicated by the larger distances between the linker DNA ends. Selective acetylation of H4 distances the linker ends for short fragments but causes them to approach each other for fragments longer than 180 bp.
authors.affiliation: Division Biophysics of Macromolecules (B040), German Cancer Research Center, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany. kt@dkfz.de
authors.forename: Katalin
authors.initials: K
authors.lastname: Tóth
authors.forename: Nathalie
authors.initials: N
authors.lastname: Brun
authors.forename: Jörg
authors.initials: J
authors.lastname: Langowski
mesh_terms: D000107:Acetylation; D020029:Base Pairing; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D031541:Fluorescence Resonance Energy Transfer; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D015636:Magnesium Chloride / Q000494:pharmacology; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D015636:Magnesium Chloride; D012965:Sodium Chloride; D004247:DNA