Development of a recombinant fowlpox virus vector-based vaccine of H5N1 subtype avian influenza.
The genetic stability of the recombinant fowlpox virus (named rFPV-HA-NA) was confirmed by serial passage on chicken embryo fibroblast (CEF) cells. The immune efficacy, safety, the minimum immunising dose, the time of immunity induced and the immune duration of the vector-based vaccine was evaluated in specific-pathogen-free (SPF) chickens. The recombinant virus vaccine containing 100 plaque form units (PFU) could induce complete protection against challenge with H5N1 highly pathogenic avian influenza virus (HPAIV). The immune efficacy, protecting chickens from clinical signs and death after challenge, was obtained one week after the immunisation with this vaccine. Protective immunity could last for 40 weeks post-immunisation. So the recombinant fowlpox vaccine is a safe and highly effective gene engineering vaccine candidate, and will be used to prevent H5 subtype avian influenza in the future.
Qiao
C
C
Animal Influenza Laboratory, Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, P R China.
Yu
K
K
Jiang
Y
Y
Li
C
C
Tian
G
G
Wang
X
X
Chen
H
H
D000818:Animals; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D005069:Evaluation Studies as Topic; D005587:Fowlpox virus / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D005585:Influenza in Birds / Q000517:prevention & control*; D011200:Poultry; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000235:genetics
D007252:Influenza Vaccines; D014614:Vaccines, Synthetic