title: Mouse disabled 1 regulates the nuclear position of neurons in a Drosophila eye model.
abstract: Nucleokinesis has recently been suggested as a critical regulator of neuronal migration. Here we show that Disabled 1 (Dab1), which is required for neuronal positioning in mammals, regulates the nuclear position of postmitotic neurons in a phosphorylation-site dependent manner. Dab1 expression in the Drosophila visual system partially rescues nuclear position defects caused by a mutation in the Dynactin subunit Glued. Furthermore, we observed that a loss-of-function allele of amyloid precursor protein (APP)-like, a kinesin cargo receptor, enhanced the severity of a Dab1 overexpression phenotype characterized by misplaced nuclei in the adult retina. In mammalian neurons, overexpression of APP reduced the ability of Reelin to induce Dab1 tyrosine phosphorylation, suggesting an antagonistic relationship between APP family members and Dab1 function. This is the first evidence that signaling which regulates Dab1 tyrosine phosphorylation determines nuclear positioning through Dab1-mediated influences on microtubule motor proteins in a subset of neurons.
authors.affiliation: Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, NIH, 35 Convent Dr., Bethesda, MD 20892, USA.
authors.forename: Albéna
authors.initials: A
authors.lastname: Pramatarova
authors.forename: Pawel G
authors.initials: PG
authors.lastname: Ochalski
authors.forename: Chi-Hon
authors.initials: CH
authors.lastname: Lee
authors.forename: Brian W
authors.initials: BW
authors.lastname: Howell
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D002465:Cell Movement; D004330:Drosophila / Q000166:cytology / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029721:Drosophila Proteins / Q000172:deficiency / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005123:Eye / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D005260:Female; D017344:Genes, Insect; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry
chemical_list: C066839:Appl protein, Drosophila; D015816:Cell Adhesion Molecules, Neuronal; C103773:Dab1 protein, mouse; D029721:Drosophila Proteins; D016326:Extracellular Matrix Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D000091042:Reelin Protein; D014443:Tyrosine; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases