Effect of oral Lactococcus lactis containing endostatin on 1, 2-dimethylhydrazine-induced colon tumor in rats.
OBJECTIVE
To investigate the effects of oral Lactococcus lactis (L lactis) containing endostatin on 1, 2-dimethylhydrazine (DMH)-induced rat colorectal cancer.


METHODS
Recombinant endostatin was produced by the expression of L lactis NZ9000. Sixty male Wistar rats were injected with DMH (40 mg/kg body weight) subcutaneously once a week for 10 wk to induce colorectal cancer. The rats were gavaged with 1 mL of endostatin at a dose of 1 x 10(8)/d and fed with the basal diet. The animals were killed after 22 wk for histopathological examination. The total time of experimental observation was 58 wk.


RESULTS
Rat endostatin protein was expressed in L lactis. Recombinant endostatin exhibited a significant effect on colorectal cancer (P<0.05). Furthermore, the mean survival time of the rats treated with endostatin was longer than that of the animals treated with DMH. There was no statistically significant difference between the rats treated with endostatin and those treated with DMH. The results showed that endostatin could not result in complete cure.
Li
Wei
W
Department of Biology, Zhaoqing College, Zhaoqing 526000, Guangdong Province, China.
Li
Chong-Bi
CB
D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D000284:Administration, Oral; D020533:Angiogenesis Inhibitors / Q000235:genetics* / Q000627:therapeutic use*; D000818:Animals; D001483:Base Sequence; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D004274:DNA, Recombinant / Q000235:genetics; D043169:Endostatins / Q000235:genetics* / Q000627:therapeutic use*; D015870:Gene Expression; D013294:Lactococcus lactis / Q000235:genetics*; D008297:Male; D010957:Plasmids / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000235:genetics / Q000627:therapeutic use
D020533:Angiogenesis Inhibitors; D004274:DNA, Recombinant; D043169:Endostatins; D011994:Recombinant Proteins; D019813:1,2-Dimethylhydrazine