title: Metabolic engineering of coenzyme Q by modification of isoprenoid side chain in plant.
abstract: Coenzyme Q (CoQ), an electron transfer molecule in the respiratory chain and a lipid-soluble antioxidant, is present in almost all organisms. Most cereal crops produce CoQ9, which has nine isoprene units. CoQ10, with 10 isoprene units, is a very popular food supplement. Here, we report the genetic engineering of rice to produce CoQ10 using the gene for decaprenyl diphosphate synthase (DdsA). The production of CoQ9 was almost completely replaced with that of CoQ10, despite the presence of endogenous CoQ9 synthesis. DdsA designed to express at the mitochondria increased accumulation of total CoQ amount in seeds.
authors.affiliation: Department of Genetic Diversity, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: Sakiko
authors.initials: S
authors.lastname: Takahashi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ogiyama
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kusano
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shimada
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawamukai
authors.forename: Koh-ichi
authors.initials: K
authors.lastname: Kadowaki
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D000975:Antioxidants / Q000502:physiology*; D001483:Base Sequence; D003067:Coenzymes; D018556:Crops, Agricultural / Q000201:enzymology / Q000235:genetics; D004579:Electron Transport / Q000502:physiology; D005818:Genetic Engineering / Q000379:methods; D008928:Mitochondria / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000201:enzymology / Q000235:genetics*; D013729:Terpenes / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000975:Antioxidants; D003067:Coenzymes; D013729:Terpenes; D014451:Ubiquinone; D019883:Alkyl and Aryl Transferases; C039821:decaprenyl pyrophosphate synthetase; C024989:coenzyme Q10; C030571:ubiquinone 9