title: New method for HPLC separation and fluorescence detection of malonaldehyde in normal human plasma.
abstract: A new method for the detection of free and total malonaldehyde (MDA) in human plasma samples based on the derivatization of MDA with 9-fluorenylmethoxycarbonyl hydrazine (FMOC-hydrazine) in an acidic medium was developed. Derivatization was achieved after 4 h at 50 degrees C. The derivatized samples were analyzed by HPLC using a reversed-phase C18 column with fluorescence detection (Ex=270 nm, Em=310 nm). The benefit of this direct injection of deproteinized plasma is to avoid the use of an internal standard. The detection limit was 0.1 pmol (4.0 nmol/L). The recovery of MDA spiked in different human plasma samples was 95.3% (n=25; R.S.D. 5.1%) for the hydrolysation procedure. The total and free MDA in plasma of 15 healthy male volunteers are 426+/-29.8 nmol/L and 153+/-9.6 nmol/L, respectively.
authors.affiliation: Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, PR China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Mao
authors.forename: Hongwu
authors.initials: H
authors.lastname: Zhang
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Renen
authors.initials: R
authors.lastname: Zhang
authors.forename: Guoquan
authors.initials: G
authors.lastname: Liu
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D012015:Reference Standards; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D008315:Malondialdehyde