Potential of KM+ lectin in immunization against Leishmania amazonensis infection.
In the present study we evaluated Canavalia brasiliensis (ConBr), Pisum arvense (PAA) and Artocarpus integrifolia (KM+) lectins as immunostimulatory molecules in vaccination against Leishmania amazonensis infection. Although they induced IFN-gamma production, the combination of the lectins with SLA antigen did not lead to lesion reduction. However, parasite load was largely reduced in mice immunized with KM+ lectin and SLA. KM+ induced a smaller inflammatory reaction in the air pouch model and was able to inhibit differentiation of dendritic cells (BMDC), but to induce maturation by enhancing the expression of MHC II, CD80 and CD86. These observations indicate the modulatory role of plant lectins in leishmaniasis vaccination may be related to their action on the initial innate response.
Teixeira
Clarissa R
CR
Centro de Pesquisas Gonçalo Moniz-Fiocruz and Faculdade de Medicina da Bahia, Rua Waldemar Falcão 121, Salvador, BA 40295-001, Brazil.
Cavassani
Karen A
KA
Gomes
Regis B
RB
Teixeira
Maria Jania
MJ
Roque-Barreira
Maria-Cristina
MC
Cavada
Benildo S
BS
da Silva
João Santana
JS
Barral
Aldina
A
Barral-Netto
Manoel
M
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000825:Animal Structures / Q000469:parasitology; D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D031622:Artocarpus; D032449:Canavalia; D003713:Dendritic Cells / Q000737:chemistry / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D007891:Leishmania / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology* / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D037121:Plant Lectins / Q000008:administration & dosage / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D000953:Antigens, Protozoan; D000949:Histocompatibility Antigens Class II; D037121:Plant Lectins; C039268:lectin, Canavalia; C036292:pea lectin; D018664:Interleukin-12; D007371:Interferon-gamma