Fluoroquinolone resistance linked to both gyrA and parC mutations in the quinolone resistance-determining region of Shigella dysenteriae type 1.
We examined the quinolone resistance-determining region (QRDR) of gyrA, gyrB, and parC of recently isolated fluoroquinolone-resistant S. dysenteriae type 1 strains from south Asia and compared data with fluoroquinolone-susceptible strains associated with previous epidemics of 1978, 1984, and 1994. In fluoroquinolone-resistant strains, double mutations (Ser83-->Leu, Asp87-->Asn or Gly) and a single mutation (Ser80-->Ile) were detected in the QRDRs of gyrA and parC, respectively.
Talukder
Kaisar A
KA
ICDDR, B: Centre for Health and Population Research, GPO Box-128, Dhaka, 1000, Bangladesh. kaisar@icddrb.org
Khajanchi
Bijay K
BK
Islam
Mohammad A
MA
Islam
Zhahirul
Z
Dutta
Dilip K
DK
Rahman
Mustafizur
M
Watanabe
Haruo
H
Nair
Gopinath B
GB
Sack
David A
DA
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001210:Asia, Southeastern; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004405:Dysentery, Bacillary / Q000382:microbiology; D024841:Fluoroquinolones / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D015363:Quinolones / Q000494:pharmacology*; D012762:Shigella dysenteriae / Q000187:drug effects* / Q000235:genetics*
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D015363:Quinolones; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase