Upregulation of type I interleukin-1 receptor after traumatic spinal cord injury in adult rats.
Post-traumatic inflammation response has been implicated in secondary injury mechanisms after spinal cord injury (SCI). Interleukin-1 (IL-1) is a key inflammatory mediator that is increasingly expressed after SCI. The action of IL-1 is mediated through its functional receptor, type I interleukin-1 receptor (IL-1RI). However, whether this receptor is expressed after SCI remains to be elucidated. In the present study, the temporospatial expression of IL-1RI was detected in rats that received a moderate contusive SCI (a 10 g rod dropped at a height of 12.5 mm) at the ninth to tenth thoracic vertebral level using a widely used New York University impact device. Our study demonstrated that IL-1RI was slightly increased at 4 h post-injury compared to the normal or sham-operated controls, reached the peak at 8 h at mRNA level (4.44-fold, P<0.01) and 1 d at protein level (2.62-fold, P<0.01). IL-1RI remained at its elevated levels for a relatively long duration (4 h-7 days). Spatially, IL-1RI was observed throughout the entire length of a 10 mm-long cord segment containing the injury epicenter. Colocalization of IL-1RI was found in neurons, oligodendrocytes, astrocytes, and activated microglia. Our results suggest that the elevated expression of IL-1RI after SCI may contribute to posttraumatic inflammation responses of IL-1.
Wang
Xiao-Fei
XF
Department of Neurobiology, Shanghai Second Medical University, 280 South Chong-Qing Road, 200025, Shanghai, People's Republic of China.
Huang
Li-Dong
LD
Yu
Pan-Pan
PP
Hu
Jian-Guo
JG
Yin
Lan
L
Wang
Li
L
Xu
Xiao-Ming
XM
Lu
Pei-Hua
PH
D000818:Animals; D005260:Female; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism; D009187:Myelitis / Q000378:metabolism* / Q000503:physiopathology; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D053573:Receptors, Interleukin-1 Type I / Q000235:genetics* / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology*
D007375:Interleukin-1; D012333:RNA, Messenger; D053573:Receptors, Interleukin-1 Type I