title: Osteoprogenitor response to semi-ordered and random nanotopographies.
abstract: In bone tissue engineering, it is desirable to use materials to control the differentiation of mesenchymal stem cell populations in order to gain direct bone apposition to implant materials. It has been known for a number of years that microtopography can alter cell adhesion, proliferation and gene expression. More recently, the literature reveals that nanotopography is also of importance. Here, the reaction of primary human osteoprogenitor cell populations to nanotopographies down to 10 nm in size is considered. The topographies were originally produced by colloidal lithography and polymer demixing on silicon and then embossed (through an intermediate nickel shim) into polymethylmethacrylate. The biological testing considered cell morphology (image analysis of cell spreading and scanning electron microscopy), cell cytoskleton and adhesion formation (fluorescent staining of actin, tubulin, vimentin and vinculin) and then subsequent cell growth and differentiation (fluorescent staining of osteocalcin and osteopontin). The results demonstrated that the nanotopographies stimulated the osteoprogenitor cell differentiation towards an osteoblastic phenotype.
authors.affiliation: Centre for Cell Engineering, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, UK. m.dalby@bio.gla.ac.uk
authors.forename: Matthew John
authors.initials: MJ
authors.lastname: Dalby
authors.forename: David
authors.initials: D
authors.lastname: McCloy
authors.forename: Mary
authors.initials: M
authors.lastname: Robertson
authors.forename: Hossein
authors.initials: H
authors.lastname: Agheli
authors.forename: Duncan
authors.initials: D
authors.lastname: Sutherland
authors.forename: Stanley
authors.initials: S
authors.lastname: Affrossman
authors.forename: Richard O C
authors.initials: RO
authors.lastname: Oreffo
mesh_terms: D000368:Aged; D018786:Bone Substitutes / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D018786:Bone Substitutes; D019904:Polymethyl Methacrylate