[Development of transgenic oilseed plants resistant to glyphosate and insects].
In this study, the aroA-M12 gene encoding bacterial 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and the Btslm recombinant gene encoding Bacillus thuringiensis (Bt) toxin gene were introduced into a Brassica napus variety, Xiangyou No. 15, via Agrobacterium-mediated transformation using glyphosate as a selectable agent. PCR amplification and Southern blot analyses of T0 transgenic plants showed that the alien genes were transferred and integrated stably into the genome of Xiangyou No. 15. Western blot further confirmed that the alien genes were expressed in these plants. Transgenic plants with tolerance to both glyphosate and the tested pest were demonstrated by bioassays. Our result also clearly shows that the aroA-M12 gene can be used as an efficient selectable marker gene instead of antibiotic resistant markers in plant transformation.
Wang
Jing-Xue
JX
The Key Laboratory of Gene Engingeering of Ministry of Education, Sun Tat-sen University, Guangzhou 510275, China. jingxuew@hotmail.com
Zhao
Fu-Yong
FY
Xu
Pei-Lin
PL
Tian
Ying-Chuan
YC
D000818:Animals; D001937:Brassica / Q000469:parasitology*; D005998:Glycine / Q000031:analogs & derivatives*; D007313:Insecta; D021261:Parasitic Sensitivity Tests; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified / Q000469:parasitology*; D012639:Seeds / Q000737:chemistry*; D000097797:Glyphosate
D005998:Glycine; D000097797:Glyphosate