Lipid malabsorption persists after weaning in rats whose dams were given GLP-2 and dexamethasone.
Glucagon-like peptide-2 (GLP-2) enhances intestinal growth and absorption in mature animals, and glucocorticosteroids (GC) increase the sugar and lipid uptake in adult animals. However, the role of GC and GLP-2 in the ontogeny of lipid absorption is unknown. We hypothesized that GLP-2 and the GC dexamethasone (DEX), when administrated to rat dams during pregnancy and lactation, would enhance lipid uptake in the offspring. Rat dams were treated in the last 10 d of pregnancy and during lactation with GLP-2 [0.1 microg/g/d subcutaneous (sc)], DEX (0.128 microg/g/d sc), GLP-2 + DEX, or a placebo. Sucklings were sacrificed at 19-21 d of age, and weanlings were sacrificed 4 wk later. Lipid uptake was assessed using an in vitro ring uptake method. Although DEX and GLP-2 + DEX increased the jejunal mass, the jejunal lipid uptake was unchanged. In contrast, GLP-2, DEX, and GLP-2 + DEX reduced the ileal lipid uptake in suckling and weanling rats. This reduction was not due to alterations in intestinal morphology or to changes in fatty acid-binding protein abundance, but it was partially explained by an increase in the effective resistance of the intestinal unstirred water layer. In sucklings, DEX dramatically reduced the jejunal lipid uptake to levels similar to those seen in weanlings, such that the normal ontogenic decline in lipid uptake was not observed. Giving dams GLP-2 or DEX during pregnancy and lactation reduced lipid uptake in the offspring, and this persisted for at least 1 mon. The impact this may have on the nutritional well-being of the animal in later life is unknown.
Iordache
Claudiu
C
Nutrition and Metabolism Group, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada.
Drozdowski
Laurie A
LA
Clandinin
M Tom
MT
Wild
Gary
G
Todd
Zoe
Z
Thomson
Alan B R
AB
D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D053767:Glucagon-Like Peptide 2; D004763:Glucagon-Like Peptides / Q000502:physiology*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism*; D050356:Lipid Metabolism; D008286:Malabsorption Syndromes / Q000378:metabolism*; D051381:Rats
D053767:Glucagon-Like Peptide 2; D004763:Glucagon-Like Peptides; D003907:Dexamethasone