Effect and mechanism of total saponin of Dioscorea on animal experimental hyperuricemia.
In this study, we investigated the effects and mechanisms of Total Saponin of Dioscorea (TSD) on animal experimental hyperuricemia. Mouse and rat hyperuricemic models were made by orally administering yeast extract paste once a day (30 and 20 g/kg, respectively), for 7 days. Yeast would disturb normal purine metabolism by increasing xanthine oxidase (XOD) activity and generating large quantities of uric acid. This model is similar to human hyperuricemia, which is induced by high-protein diets, due to a purine and nucleic acid metabolic disturbance. Another mouse hyperuricemia model was generated by intraperitoneal injection once with uric acid 250 mg/kg or potassium oxonate 300 mg/kg. Potassium oxonate, a urate oxidase inhibitor, can raise the serum uric acid level by inhibiting the decomposition of uric acid. Likewise, injecting uric acid can also increase serum uric acid concentration. The concentration of uric acid in serum or urine was detected by the phosphotungstic acid method, and the activity of XOD was assayed by a test kit. The results showed that TSD (240, 120 and 60 mg/kg, ig) could significantly lower the level of serum uric acid in hyperuricemic mice. TSD (120 and 60 mg/kg, ig) could also lower the level of serum uric acid in hyperuricemic rats, reduce the activity of XOD in the serum and liver of hyperuricemic rats, and increase the level of urine uric acid concentration as well as 24-hour total uric acid excretion. In conclusion, TSD possesses a potent anti-hyperuricemic effect on hyperuricemic animals, and the mechanism may be relevant in accelerating the excretion and decreasing the production of uric acid.
Chen
Guang-Liang
GL
Institute of Clinical Pharmacology, Anhui Medical University, Hefei, China 230032, China. chguangl@163.com
Wei
Wei
W
Xu
Shu-Yun
SY
D000818:Animals; D027742:Dioscorea; D004195:Disease Models, Animal; D033461:Hyperuricemia / Q000139:chemically induced / Q000188:drug therapy*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D010094:Oxonic Acid / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000494:pharmacology*; D014527:Uric Acid / Q000008:administration & dosage / Q000097:blood / Q000652:urine; D014969:Xanthine Oxidase / Q000187:drug effects / Q000378:metabolism; D015003:Yeasts
D012503:Saponins; D014527:Uric Acid; C489337:potassium oxonate; D010094:Oxonic Acid; D014969:Xanthine Oxidase