title: Lignans from cell suspension cultures of Phyllanthus niruri, an Indonesian medicinal plant.
abstract: Cell suspension cultures of Phyllanthus niruri were used to study the lignan profiles and biosynthesis. Suspension cultures yielded two lignans: the new cubebin dimethyl ether (1) and urinatetralin (2), a new lignan from P. niruri, but reported earlier from P. urinaria. This is the first report of cell suspension cultures of P. niruri that successfully produce lignans. Feeding 0.5 mM ferulic acid or 0.5 mM caffeic acid, being early precursors of lignan biosynthesis, resulted in an increase up to 0.7 mg g(-1) DW of 1 (control value 0.1 mg g(-1) DW) and up to 0.3 mg g(-1) DW of 2 (control value 0.2 mg g(-1) DW). Comparison of the lignan profiles of cell suspensions, callus cultures, aerial plant parts, roots, and seeds showed significant differences.
authors.affiliation: Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy (GRIP), Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.lastname: Elfahmi
authors.forename: Sieb
authors.initials: S
authors.lastname: Batterman
authors.forename: Albert
authors.initials: A
authors.lastname: Koulman
authors.forename: Thomas
authors.initials: T
authors.lastname: Hackl
authors.forename: Rein
authors.initials: R
authors.lastname: Bos
authors.forename: Oliver
authors.initials: O
authors.lastname: Kayser
authors.forename: Herman J
authors.initials: HJ
authors.lastname: Woerdenbag
authors.forename: Wim J
authors.initials: WJ
authors.lastname: Quax
mesh_terms: D002109:Caffeic Acids / Q000494:pharmacology; D003373:Coumaric Acids / Q000494:pharmacology; D017705:Lignans / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D029803:Phyllanthus / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000737:chemistry
chemical_list: D002109:Caffeic Acids; D003373:Coumaric Acids; D017705:Lignans; C508538:cubebin dimethyl ether; C508539:urinatetralin; C004999:ferulic acid; C040048:caffeic acid