title: One of the three proteinase inhibitor genes newly identified in the Brassica napus genome codes for an inhibitor of glutamyl endopeptidase.
abstract: Three proteinase inhibitor genes have been identified in the rapeseed (Brassica napus) genome. They are highly homologous to other genes of the mustard inhibitor (MSI) family of proteinase inhibitors characteristic of Cruciferae. In germinating seeds, only the transcript of one gene, coding for a trypsin inhibitor, is detectable by Northern analysis. The other two genes are transcribed at basal levels detectable only by reverse transcription PCR. One of the other two genes (rti-2) encodes a polypeptide with a glutamic residue in the P1 position, characteristic of glutamyl proteinase inhibitors. The recombinant RTI-2 protein strongly inhibits (Ki=44 nM) a glutamyl proteinase from Streptomyces griseus.
authors.affiliation: Institute of Biomembranes and Bioenergetic, Italian National Research Council, CNR, Via Amendola, 165/A, 70126 Bari, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: De Leo
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Volpicella
authors.forename: Marta
authors.initials: M
authors.lastname: Sciancalepore
authors.forename: Raffaele
authors.initials: R
authors.lastname: Gallerani
authors.forename: Luigi R
authors.initials: LR
authors.lastname: Ceci
mesh_terms: D015152:Blotting, Northern / Q000379:methods; D029688:Brassica napus / Q000235:genetics* / Q000254:growth & development; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D018525:Germination / Q000502:physiology*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D012697:Serine Endopeptidases / Q000378:metabolism; D013305:Streptomyces griseus / Q000201:enzymology; D014361:Trypsin Inhibitors / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D014361:Trypsin Inhibitors; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase