Sequence-based typing reveals a novel DLA-88 allele, DLA-88*04501, in a beagle family.
The dog is an important animal model for solid organ as well as stem cell allo transplantation. Methods such as cellular and serological typing and more recently sequence-based typing (SBT) have been used to discriminate tissue antigen disparity of donor and recipient. We applied SBT for the canine class I (DLA-88) and class II (DLA-DRB1) genes in beagle families prior stem cell transplantation. A novel DLA-88 (DLA-88*04501) allele in combination with a DLA-DRB1*01901 allele was found. Sequence comparison of exons 2 and 3 of the novel allele revealed most sequence identity to the DLA-88*01301 allele (96.15% identity at the nucleotide and 90.65% identity at the protein level).
Hardt
C
C
Institute for Immunology, University Hospital of Essen, Germany. cornelia.hardt@medizin.uni-essen.de
Ferencik
S
S
Tak
R
R
Hoogerbrugge
P M
PM
Wagner
V
V
Grosse-Wilde
H
H
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004285:Dogs / Q000235:genetics* / Q000276:immunology; D014644:Genetic Variation; D006239:Haplotypes; D006649:Histocompatibility Antigens / Q000235:genetics*; D008285:Major Histocompatibility Complex / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D035781:Siblings
D006649:Histocompatibility Antigens