Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes by fluorescence differential display.
Using fluorescence differential display, cDNAs specifically expressed at the primordial stage of fruiting body development were isolated from the basidiomycete, Flammulina velutipes. Seventy-five cDNAs were sequenced and compared with the amino-acid sequences of proteins in the database by BLASTX search. Significant similarity was found for 29 cDNAs coding for proteins with known function, GTP-binding protein, growth factor, ubiquitin-proteasome, cytochrome P450 and hydrophobin, all of which would be associated with fruiting body development. Seventeen cDNAs were not similar to proteins in the database and may represent unique genes that play specific roles in the process of fruiting in F. velutipes.
Yamada
Masato
M
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.
Sakuraba
Sou
S
Shibata
Kou
K
Taguchi
Goro
G
Inatomi
Satoshi
S
Okazaki
Mitsuo
M
Shimosaka
Makoto
M
D000363:Agaricales / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D048690:Fruiting Bodies, Fungal / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D005656:Fungal Proteins