Hyperbaric oxygenation and antioxidant vitamin combination reduces ischemia-reperfusion injury in a rat epigastric island skin-flap model.
Reperfusion injury, caused by free oxygen radicals, is a chain of events that occurs in tissues exposed to a constant period of ischemia. The antioxidant vitamins E and C (VEC) and hyperbaric oxygenation (HBO) have beneficial effects in treating ischemic tissues following skin flap operations. In our study, we aimed to compare the effects of VEC and/or HBO in ischemia-reperfusion injury induced by free oxygen radicals in an experimental rat epigastric island skin-flap model. Eight hours of ischemia was provided by clamping the inferior epigastric pedicle following the flap elevation. The flap survey was determined to be 28.6% in controls, 59.2% in HBO group, 66.3% in VEC + HBO group, and 82% in VEC group (p < 0.05). We conclude that although HBO and/or VEC increased the flap viability significantly by reducing ischemia-reperfusion injury, the most promising results were obtained in the antioxidant vitamins group.
Tomur
Ahmet
A
Department of Physiology, GATA Military Medical School Etlik, Ankara.
Etlik
Ozdal
O
Gundogan
Nimet Unay
NU
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000097:blood / Q000494:pharmacology; D005260:Female; D006931:Hyperbaric Oxygenation; D015227:Lipid Peroxidation / Q000187:drug effects; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000517:prevention & control*; D013524:Surgical Flaps; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D014815:Vitamins; D014810:Vitamin E; D000255:Adenosine Triphosphate; D001205:Ascorbic Acid; D010100:Oxygen