title: Comparison of soxhlet, ultrasound-assisted and pressurized liquid extraction of terpenes, fatty acids and Vitamin E from Piper gaudichaudianum Kunth.
abstract: This paper describes a comparative study of extraction methods of terpenes (terpenic alcohols and phytosterols), fatty acids and Vitamin E from leaves of Piper gaudichaudianum Kunth. The analysis of extracts was done by gas chromatography with mass spectrometric detection. The identification and quantification was made by co-injections of the extract with certified standards. The use of pressurized liquid extraction (PLE; Dionex trade name: ASE, for accelerated solvent extraction) decrease significantly the total time of extraction, the amount of solvent and the manipulation of sample and solvents in comparison with soxhlet (SE) and ultrasound-assisted (USE). In addition, PLE was more effective for the extractions of terpenes (terpenic alcohols and phytosterols), fatty acids and Vitamin E.
authors.affiliation: Laboratório de Genética Toxicológica, ULBRA, Canoas, RS-Brazil; Universidad de La Habana, Cuba.
authors.forename: Valéria Flores
authors.initials: VF
authors.lastname: Péres
authors.forename: Jenifer
authors.initials: J
authors.lastname: Saffi
authors.forename: Maria Inês S
authors.initials: MI
authors.lastname: Melecchi
authors.forename: Fernanda C
authors.initials: FC
authors.lastname: Abad
authors.forename: Rosângela
authors.initials: R
authors.lastname: de Assis Jacques
authors.forename: Migdalia M
authors.initials: MM
authors.lastname: Martinez
authors.forename: Eniz Conceição
authors.initials: EC
authors.lastname: Oliveira
authors.forename: Elina B
authors.initials: EB
authors.lastname: Caramão
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D005227:Fatty Acids / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D031701:Piper / Q000737:chemistry*; D011312:Pressure; D013729:Terpenes / Q000302:isolation & purification*; D014465:Ultrasonics; D014810:Vitamin E / Q000302:isolation & purification*
chemical_list: D005227:Fatty Acids; D013729:Terpenes; D014810:Vitamin E