title: Rapid analysis of stilbenes and derivatives from downy mildew-infected grapevine leaves by liquid chromatography-atmospheric pressure photoionisation mass spectrometry.
abstract: Resveratrol, trans-epsilon-viniferin and trans-delta-viniferin are the major stilbenes induced in downy mildew infected grapevine leaves. In addition, nine minor polyphenolic compounds, described as stilbenes derivatives, have been separated and detected among known stilbenes after a methanolic microextraction of small pieces (1-2 mg) from infected grapevine leaves with a rapid, qualitative and optimized HPLC method coupled to mass spectrometry using atmospheric pressure photoionisation (APPI-MS(n)). The characterization of unknown stilbenic derivatives as six resveratrol dimers, two dimethylated resveratrol dimers and a resveratrol trimer are reported. Therefore, structures have been proposed for the dimethylated resveratrol dimers. Use of an easy sample treatment and the LC-APPI-MS(n) method results in spectral data of these minor naturally occurring viniferin analogues.
authors.affiliation: Institute of Chemistry, University of Neuchatel, Rue Emile Argand 11, CH-2009 Neuchatel, Switzerland.
authors.forename: J Bernard
authors.initials: JB
authors.lastname: Jean-Denis
authors.forename: Roger
authors.initials: R
authors.lastname: Pezet
authors.forename: Raffaele
authors.initials: R
authors.lastname: Tabacchi
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D005419:Flavonoids / Q000032:analysis; D010636:Phenols / Q000032:analysis; D010935:Plant Diseases; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013267:Stilbenes / Q000032:analysis*; D027843:Vitis / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013267:Stilbenes