title: A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium.
abstract: A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His6-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signal peptide, and recovered by one-step affinity chromatography.
authors.affiliation: Institute of Microbiology, Technical University Braunschweig, Spielmannstrasse 7, D-38106 Braunschweig, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Malten
authors.forename: Rebekka
authors.initials: R
authors.lastname: Biedendieck
authors.forename: Martin
authors.initials: M
authors.lastname: Gamer
authors.forename: Ann-Christin
authors.initials: AC
authors.lastname: Drews
authors.forename: Simon
authors.initials: S
authors.lastname: Stammen
authors.forename: Klaus
authors.initials: K
authors.lastname: Buchholz
authors.forename: Lubbert
authors.initials: L
authors.lastname: Dijkhuizen
authors.forename: Dieter
authors.initials: D
authors.lastname: Jahn
mesh_terms: D000345:Affinity Labels; D001410:Bacillus megaterium / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D002846:Chromatography, Affinity; D003470:Culture Media; D015870:Gene Expression; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006602:Hexosyltransferases / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D052200:Limosilactobacillus reuteri / Q000201:enzymology / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000345:Affinity Labels; D001426:Bacterial Proteins; D003470:Culture Media; C079999:LipA protein, Bacteria; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D006602:Hexosyltransferases; C021513:levansucrase