Stationary phases in the screening of drug/impurity profiles and in their separation method development: identification of columns with different and similar selectivities.
The classification or characterization of stationary phases based on chromatographic parameters, in general, requires different test solutes/mixtures and several mobile phases. To simplify the classification/characterization of reversed-phase liquid chromatographic columns, to be used in separating drug/impurity profiles, a new test procedure was proposed. It consists of injecting two mixtures of relatively similar active substances applying a standard gradient. The aim was to evaluate from this approach the selectivity differences and overall separation quality of newly tested columns compared to that in an earlier selected set of eight stationary phases. The selectivity differences of the columns were evaluated by correlation coefficient-based weighted-average-linkage dendrograms and color maps. Derringer's desirability functions were used to rank similar stationary phases according to their overall separation quality. Four columns of 27 examined were, for instance, considered different from the earlier selected eight and could be added to the selection. A number of tested stationary phases might be considered as alternatives for some from the initial set. For three columns the newly tested stationary phases did not contain alternatives.
Van Gyseghem
E
E
Department of Analytical Chemistry and Pharmaceutical Technology, Vrije Universiteit Brussel-VUB, Laarbeeklaan 103, B-1090 Brussels, Belgium.
Jimidar
M
M
Sneyers
R
R
De Smet
M
M
Verhoeven
E
E
Vander Heyden
Y
Y
D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry / Q000379:methods; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D004364:Pharmaceutical Preparations