The employment of strontium to activate mouse oocytes: effects on spermatid-injection outcome.
The present research investigated the effects of various strontium concentrations, in combination with different incubation periods, on mouse parthenogentic oocyte activation and blastocyst development. The results for blastocyst development showed a trend indicating that 10 mM strontium for 3 h was the optimal strontium protocol. Ethanol, an agent that incites oocyte activation via a monotonic rise in calcium, was employed as a control. The outcome of blastocyst formation arising from parthenogenic ethanol activation was significantly less (P < 0.001) than that achieved by the optimal strontium protocol. To assess the impact of strontium oocyte activation on embryo viability following fertilization with immature germ cells, the protocol of 10 mM strontium for 3 h was applied to oocytes injected with round spermatids and then compared with other protocols. The results indicate that following round-spermatid injection the benefits derived from strontium artificial oocyte activation are evident during both pre- and post-implantation development. However, in order to adjust the protocol to the most effective round-spermatid injection in relation to the oocyte cell cycle, injection was done 1.5 h after strontium activation followed by another 1.5 h activation in strontium. The implementation of round-spermatid injection in combination with this oocyte-activation protocol led to live-birth outcomes not significantly different to those outcomes obtained by mature spermatozoa.
Loren
Jean
J
Monash Immunology and Stem Cell Laboratories, Level 3, STRIP 1- Building 75, Monash University, Wellington Rd., Clayton, Australia, 3800.
Lacham-Kaplan
Orly
O
D000818:Animals; D001755:Blastocyst / Q000502:physiology; D004624:Embryo Transfer; D047108:Embryonic Development; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D008297:Male; D008540:Meiosis / Q000187:drug effects*; D051379:Mice; D009865:Oocytes / Q000187:drug effects*; D010312:Parthenogenesis; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic; D013084:Sperm-Ovum Interactions; D013087:Spermatids; D013324:Strontium / Q000494:pharmacology*
D000431:Ethanol; D013324:Strontium