title: [Expression of IgVH and B7-1 in proteome of the human colorectal carcinoma cell lines].
abstract: OBJECTIVE
  To conduct a proteomic analysis of human colorectal carcinoma cell lines LS174T and SW480 by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).
  
  
  METHODS
  The total proteins of human colorectal carcinoma cell lines LS174T and SW480 were separated with 2-DE using immobilized pH gradient strips and analyzed by MALDI-TOF-MS to obtain peptide mass fingerprints (PMFs). Proteins were identified by using Mascot software to search protein databases.
  
  
  RESULTS
  Good resolution 2-DE maps were obtained. Some proteins including immunoglobulin heavy chain variable region (IgVH) and co-stimulatory molecule B7-1 were identified. IgVH and B7-1 were confirmed by electrospray ionization tandem spectrometry (ESI-MS/MS) and immunocytochemistry.
  
  
  CONCLUSIONS
  There are IgVH and B7-1 expressions in human colorectal carcinoma cell lines LS174T and SW480. Results obtained will help to elucidate the mechanisms of tumor immune escape.
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Cancer Research, School of Life Science & Technology, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Ying-tao
authors.initials: YT
authors.lastname: Zhang
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Geng
authors.forename: Bao-chang
authors.initials: BC
authors.lastname: Lai
authors.forename: Lü-sheng
authors.initials: LS
authors.lastname: Si
authors.forename: Yi-li
authors.initials: YL
authors.lastname: Wang
mesh_terms: D018122:B7-1 Antigen / Q000096:biosynthesis* / Q000235:genetics; D002461:Cell Line, Transformed; D015179:Colorectal Neoplasms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis* / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D010449:Peptide Mapping; D020543:Proteome / Q000302:isolation & purification
chemical_list: D018122:B7-1 Antigen; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D009363:Neoplasm Proteins; D020543:Proteome