Pregnancy and delivery after in vitro maturation of naked ICSI-GV oocytes with GH and transfer of a frozen thawed blastocyst: case report.
OBJECTIVE
To determine if GV oocytes, collected at the time of ICSI, can be matured in vitro and rescued for therapeutic treatment. A patient for whom all the collected oocytes at the GV stage after a classical COH protocol were matured in vitro with GH.


METHODS
All the naked oocytes were matured in a culture medium (ISM2) containing 15% patient serum +1.6 units of GH (Saizen) per millilitre. Oocytes were incubated overnight at 37 degrees C. The MII oocytes obtained were micro-injected. A fresh transfer was performed and a supernumerary blastocyst was frozen.


RESULTS
The patient was pregnant and delivered a healthy girl after transfer of the frozen/thawed blastocyst. The baby girl is now 2 years old.


CONCLUSIONS
In vitro maturation with GH allows rescuing naked GV oocytes collected at the time of ICSI. GH action does not pass through the cumulus cells. According to the possible lack of synchrony between the embryo and the uterus, we recommend to freeze the embryos obtained and to replace them in a controlled cycle.
Menezo
Yves J R
YJ
Centre d'AMP de la clinique du Val d'Ouest, 39 chemin de la Vernique, 69132 Ecully, France. yves.menezo@club-internet.fr
Nicollet
Bernard
B
Rollet
Jacques
J
Hazout
André
A
D000328:Adult; D001755:Blastocyst; D015925:Cryopreservation; D036861:Delivery, Obstetric; D004624:Embryo Transfer; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D050498:Live Birth; D009865:Oocytes / Q000187:drug effects* / Q000637:transplantation; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*
D013006:Growth Hormone