In vitro studies of the influence of certain enzymes on the detoxification of acrylamide and glycidamide in blood.
Several enzymes involved in the metabolism of xenobiotic substances are polymorphic in humans. Inter-individual differences in response to certain chemicals, such as acrylamide, as a result of such genetic polymorphisms might affect health-risk assessments. Detoxification by, for example, conjugation with glutathione (GSH) will decrease the concentration. The dose of the compound and enzymes that enhance the conjugation with GSH will increase the detoxification rate. The dose of acrylamide or glycidamide has been measured in blood samples from individuals with defined genotypes for the glutathione transferases GSTT1 and GSTM1 after in vitro incubation with these compounds. The results indicate that these enzymes have no significant effect on the blood dose, measured as Hb adducts over time, after exposure to acrylamide or glycidamide.
Paulsson
Birgit
B
Dept. of Environmental Chemistry, Stockholm University, SE-106 91 Stockholm, Sweden. birgit.paulsson@mk.su.se
Warholm
Margareta
M
Rannug
Agneta
A
Törnqvist
Margareta
M
D020106:Acrylamide / Q000097:blood* / Q000633:toxicity*; D004852:Epoxy Compounds / Q000097:blood* / Q000633:toxicity*; D005838:Genotype; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008658:Inactivation, Metabolic; D011110:Polymorphism, Genetic; D012306:Risk; D018570:Risk Assessment
D020106:Acrylamide; D004852:Epoxy Compounds; D005978:Glutathione; D005982:Glutathione Transferase; D006454:Hemoglobins; C071834:glycidamide; C413545:glutathione S-transferase T1