Delayed wound healing and epidermal hyperproliferation in mice lacking JunB in the skin.
The cutaneous response to injury and stress comprises a temporary change in the balance between epidermal proliferation and differentiation as well as an activation of the immune system. Soluble factors play an important role in the regulation of these complex processes by coordinating the intercellular communication between keratinocytes, fibroblasts, and inflammatory cells. In this study, we demonstrate that JunB, a member of the activator protein-1 transcription factor family, is an important regulator of cytokine expression and thus critically involved in the cutaneous response to injury and stress. Mice lacking JunB in the skin develop normally, indicating that JunB is neither required for cutaneous organogenesis, nor homeostasis. However, upon wounding and treatment with the phorbol ester 12-O-decanoyl-phorbol-13-acetate, JunB-deficiency in the skin likewise resulted in pronounced epidermal hyperproliferation, disturbed differentiation, and prolonged inflammation. Furthermore, delayed tissue remodelling was observed during wound healing. These phenotypic skin abnormalities were associated with JunB-dependent alterations in expression levels and kinetics of important mediators of wound repair, such as granulocyte macrophage colony-stimulating factor, growth-regulated protein-1, macrophage inflammatory protein-2, and lipocalin-2 in both the dermal and epidermal compartment of the skin, and a reduced ability of wound contraction of mutant dermal fibroblasts in vitro.
Florin
Lore
L
Division of Signal Transduction and Growth Control, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Knebel
Julia
J
Zigrino
Paola
P
Vonderstrass
Birgitta
B
Mauch
Cornelia
C
Schorpp-Kistner
Marina
M
Szabowski
Axel
A
Angel
Peter
P
D000818:Animals; D049109:Cell Proliferation; D003094:Collagen / Q000235:genetics; D024042:Collagen Type I; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004817:Epidermis / Q000187:drug effects / Q000196:embryology / Q000502:physiology*; D005804:Genes, Lethal; D006706:Homeostasis / Q000235:genetics; D019426:Integrases / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*; D011995:Recombination, Genetic; D017384:Sequence Deletion; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000196:embryology; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D014764:Viral Proteins / Q000378:metabolism; D014945:Wound Healing / Q000235:genetics* / Q000276:immunology
D024042:Collagen Type I; D016207:Cytokines; D016755:Proto-Oncogene Proteins c-jun; D014764:Viral Proteins; D003094:Collagen; C045073:Cre recombinase; D019426:Integrases; D013755:Tetradecanoylphorbol Acetate