Function analysis of promoter trapping system after inserted into cotton (Gossypium hirsutum L. ) genome.
The technique of promoter trapping was developed to investigate its viability in cotton ( Gossypium hirsutum L.) functional genomics. 141 independent transformants of cotton were generated via Agrobacterium tumefaciens mediated transformation, of which 97% showed positive by PCR detection. The reporter, GUS gene, was expressed to different extent in different organs, with a frequency of 48% in roots, 9.2% in vascular bundles of stem, 5.2% in leaves, and 51% in flowers. Meanwhile, we discovered that there existed great differences in expression patterns among different transgenic lines. Their GUS expression patterns were organ- or tissue-specific or ubiquitous in all of the plants. The promoter trapping system developed here was characterized as an effective method for creating mutants with diverse reporter gene expression patterns, which laid a solid foundation for further research of functional genomics in cotton.
Jin
Shuang-Xia
SX
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China. jsx@webmail.hzau.edu.cn
Zhang
Xian-Long
XL
Nie
Yi-Chun
YC
Guo
Xiao-Ping
XP
Sun
Yu-Qiang
YQ
Huang
Chao
C
Liang
Shao-Guang
SG
D001483:Base Sequence; D017930:Genes, Reporter; D018745:Genome, Plant; D003368:Gossypium / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics* / Q000502:physiology