title: Preliminary functional characterization, cloning and primary sequence of Fastuosain, a cysteine peptidase isolated from fruits of Bromelia fastuosa.
abstract: The present work reports the characterization of Fastuosain, a novel cysteine protease of 25kDa, purified from the unripe fruits of Bromelia fastuosa, a wild South American Bromeliaceae. Proteolytic activity, measured using casein and synthetic substrates, was dependent on the presence of thiol reagents, having maximum activity at pH 7.0. The present work reports cDNA cloning of Fastuosain; cDNA was amplified by PCR using specific primers. The product was 1096pb long. Mature fastuosain has 217 residues, and with the proregion has a total length of 324 residues. Its primary sequence showed high homology with ananain(74%), stem bromelain (66%) and papain (44%).
authors.affiliation: Departamento de Química e Ciências Ambientais,IBILCE-UNESP, State University of São Paulo, São José do Rio Preto SP, Brazil.
authors.forename: Hamilton
authors.initials: H
authors.lastname: Cabral
authors.forename: Andréia M
authors.initials: AM
authors.lastname: Leopoldino
authors.forename: Eloiza H
authors.initials: EH
authors.lastname: Tajara
authors.forename: Lewis J
authors.initials: LJ
authors.lastname: Greene
authors.forename: Vitor M
authors.initials: VM
authors.lastname: Faça
authors.forename: Rogério P
authors.initials: RP
authors.lastname: Mateus
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Ceron
authors.forename: Wagner A
authors.initials: WA
authors.lastname: de Souza Judice
authors.forename: Luiz
authors.initials: L
authors.lastname: Julianod
authors.forename: Gustavo O
authors.initials: GO
authors.lastname: Bonilla-Rodriguez
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D031231:Bromelia / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D017931:DNA Primers; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D003546:Cysteine Endopeptidases; C509400:fastuosain, Bromelia fastuosa