[HLA-B gene polymorphism detected by high-resolution sequence-based typing in Guangdong Han populations].
OBJECTIVE
To investigate the genetic polymorphism of HLA-B locus in Guangdong Han population and compare the characteristic of the allele frequency distribution with that in other populations.


METHODS
A total of 562 cord blood samples from Guangzhou Cord Blood Bank were analyzed by sequence-based typing. Then the sequences encompassing exons 2, 3, and 4 for HLA-B gene were analyzed by direct sequencing of PCR products. The allele frequency distribution of HLA-B in this population was compared with that in other populations.


RESULTS
A total of 59 different HLA-B alleles were detected, and among them were 6 HLA-B alleles with frequencies higher than 5%: HLA-B*4601 (14.5%), HLA-B*400101 (14.4%), HLA-B*1502 (11.5%), HLA-B*1301 (8.6%), HLA-B*5801 (8.1%) and HLA-B*380201 (6.4%); the total frequency of these six alleles was 63.5%. At the same time, there were 30 kinds of HLA-B allele with frequencies lower than 0.5%; the total frequency of these alleles was 4.9%. Maximum variation at HLA-B was seen in the HLA-B*15 allele family (nine alleles). Comparison of the HLA-B frequencies in different populations showed a close relationship of Guandong Han population with the Chinese populations from Hong Kong and Singapore, respectively.


CONCLUSIONS
The results have shown the characteristic of HLA-B distribution and provided more accurate genotypic data that may serve as normal reference value for the Han population in Guangdong, China.
Li
Yan
Y
Guangzhou Maternal and Neonatal Hospital, Guangzhou Cord Blood Bank, Guangzhou, Guangdong 510180, P.R. China.
Tang
Xue-wei
XW
Wu
Jie-ying
JY
Yuan
Si-min
SM
Yang
Xin
X
Huang
Yi-ning
YN
Liao
Can
C
D044466:Asian People / Q000235:genetics*; D002681:China / Q000208:ethnology; D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic
D015235:HLA-B Antigens