title: Peripheral myelin protein 22 is in complex with alpha6beta4 integrin, and its absence alters the Schwann cell basal lamina.
abstract: Peripheral myelin protein 22 (PMP22) is a tetraspan membrane glycoprotein, the misexpression of which is associated with hereditary demyelinating neuropathies. Myelinating Schwann cells (SCs) produce the highest levels of PMP22, yet the function of the protein in peripheral nerve biology is unresolved. To investigate the potential roles of PMP22, we engineered a novel knock-out (-/-) mouse line by replacing the first two coding exons of pmp22 with the lacZ reporter. PMP22-deficient mice show strong beta-galactosidase reactivity in peripheral nerves, cartilage, intestines, and lungs, whereas phenotypically they display the characteristics of tomaculous neuropathy. In the absence of PMP22, myelination of peripheral nerves is delayed, and numerous axon-SC profiles show loose basal lamina, suggesting altered interactions of the glial cells with the extracellular matrix. The levels of beta4 integrin, a molecule involved in the linkage between SCs and the basal lamina, are severely reduced in nerves of PMP22-deficient mice. During early stages of myelination, PMP22 and beta4 integrin are coexpressed at the cell surface and can be coimmunoprecipitated together with laminin and alpha6 integrin. In agreement, in clone A colonic carcinoma cells, epitope-tagged PMP22 forms a complex with beta4 integrin. Together, these data indicate that PMP22 is a binding partner in the integrin/laminin complex and is involved in mediating the interaction of SCs with the extracellular environment.
authors.affiliation: Department of Neuroscience, College of Medicine, McKnight Brain Institute, University of Florida, Gainesville, Florida 32610-0244, USA.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Amici
authors.forename: William A
authors.initials: WA
authors.lastname: Dunn
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Murphy
authors.forename: Niels C
authors.initials: NC
authors.lastname: Adams
authors.forename: Nicholas W
authors.initials: NW
authors.lastname: Gale
authors.forename: David M
authors.initials: DM
authors.lastname: Valenzuela
authors.forename: George D
authors.initials: GD
authors.lastname: Yancopoulos
authors.forename: Lucia
authors.initials: L
authors.lastname: Notterpek
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism / Q000648:ultrastructure; D001665:Binding Sites; D045744:Cell Line, Tumor / Q000737:chemistry; D018920:Coculture Techniques; D003110:Colonic Neoplasms / Q000737:chemistry / Q000473:pathology; D005091:Exons / Q000235:genetics; D005727:Ganglia, Spinal / Q000166:cytology; D006801:Humans; D039161:Integrin alpha6beta4 / Q000737:chemistry / Q000378:metabolism*; D007763:Lac Operon; D007797:Laminin / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D046912:Multiprotein Complexes; D009185:Myelin Proteins / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D009928:Organ Specificity; D010523:Peripheral Nervous System Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010641:Phenotype; D011485:Protein Binding; D025941:Protein Interaction Mapping; D051381:Rats; D012583:Schwann Cells / Q000378:metabolism* / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D039161:Integrin alpha6beta4; D007797:Laminin; D046912:Multiprotein Complexes; D009185:Myelin Proteins; C073687:PMP22 protein, human; C489545:Pmp22 protein, mouse; C489546:Pmp22 protein, rat