title: Primary structure of a developmentally regulated nicotinic acetylcholine receptor protein from Drosophila.
abstract: Acetylcholine is a major excitatory neurotransmitter in the central nervous system of insects. Using DNA probes of the Torpedo nicotinic acetylcholine receptor (AChR) we have isolated two overlapping cDNA clones encoding a putative neuronal AChR protein from the fruitfly, Drosophila melanogaster. The predicted mature protein consists of 497 amino acids, has a calculated mol. wt of 57 340 and shows extensive homology to known AChR subunits from different species along its entire amino acid sequence. Northern analysis revealed a hybridizing mRNA of 3.2 kb in late embryo and in pupae. Expression of the corresponding AChR gene thus characterizes periods of neuronal differentiation in Drosophila.
authors.affiliation: Zentrum für Molekulare Biologie, Universität Heidelberg, Im Neuenheimer Feld 282, D-6900 Heidelberg, FRG.
authors.forename: I
authors.initials: I
authors.lastname: Hermans-Borgmeyer
authors.forename: D
authors.initials: D
authors.lastname: Zopf
authors.forename: R P
authors.initials: RP
authors.lastname: Ryseck
authors.forename: B
authors.initials: B
authors.lastname: Hovemann
authors.forename: H
authors.initials: H
authors.lastname: Betz
authors.forename: E D
authors.initials: ED
authors.lastname: Gundelfinger