title: Protein turnover on the scale of the proteome.
abstract: Protein turnover is a neglected dimension in postgenomic studies, defining the dynamics of changes in protein expression and forging a link between transcriptome, proteome and metabolome. Recent advances in postgenomic technologies have led to the development of new proteomic techniques to measure protein turnover on a proteome-wide scale. These methods are driven by stable isotope metabolic labeling of cells in culture or in intact animals. This review considers the merits and difficulties of different methods that allow access to proteome dynamics.
authors.affiliation: Protein Function Group, Faculty of Veterinary Science, University of Liverpool, Liverpool, L69 7ZJ, UK. doherty@liv.ac.uk
authors.forename: Mary K
authors.initials: MK
authors.lastname: Doherty
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Beynon
mesh_terms: D000818:Animals; D006801:Humans; D007553:Isotope Labeling; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D011506:Proteins; D020543:Proteome