[Study on the differentiation of marrow mesenchymal stem cells of the rats mediated by bone morphogenetic protein 2].
OBJECTIVE
To explore the effect of age and gene therapy on the differentiation of marrow mesenchymal stem cells (MSCs) of the rats.


METHODS
MSCs from the young (1-month-old), adult (9-month-old), and the aged (24-month-old) rats were expanded in culture and infected with adenovirus mediated human bone morphogenetic protein 2 gene (Ad-BMP-2). The expression of BMP-2 and osteoblastic markers such as alkaline phosphatase (ALP), collagen I (Col I), bone sialoprotein (BSP) and osteopontin (OPN) were assayed during the process of differentiation. Their abilities to induce ectopic bone formation in nude mice were also tested.


RESULTS
There was no significant difference in the expression of BMP-2 among the 3 groups. ALP activity assay and semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) demonstrated that there were no significant differences in the expression of osteoblastic markers ALP, Col- I, OPN and BSP among the 3 groups. Histomorphometric analysis indicated that there were no significant differences in the volume of the newly formed ectopic bones in nude mice among the 3 groups.


CONCLUSIONS
MSCs obtained from the aged rats can restore their osteogenic activity following human BMP-2 gene transduction,therefore provides an alternative to treating the aged bone disease.
Yue
Bing
B
Shanghai Ninth Peoples' Hospital, Shanghai Jiaotong University Medical School, Shanghai 200011, PR China.
Chen
Yanfei
Y
Tang
Tingting
T
Lu
Bin
B
Yu
Chaofeng
C
Lou
Jueren
J
Dai
Kerong
K
D000818:Animals; D030801:Animals, Genetically Modified; D001854:Bone Marrow Cells / Q000166:cytology*; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics*; D002454:Cell Differentiation; D002478:Cells, Cultured; D015316:Genetic Therapy; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D011923:Rats, Nude; D017208:Rats, Wistar
D055396:Bone Morphogenetic Protein 2