Unique NS5b hepatitis C virus gene sequence consensus database is essential for standardization of genotype determinations in multicenter epidemiological studies.
A multicenter study of NS5b hepatitis C virus (HCV) genotype determination involving 12 laboratories demonstrates that any laboratory with expertise in sequencing techniques would be able to provide a reliable HCV genotype for clinical and epidemiological purposes as long as they are provided a consensus reference sequence database.
Laperche
Syria
S
Institut National de la Transfusion Sanguine, 6 rue Alexandre-Cabanel, 75015 Paris, France.
Saune
Karine
K
Dény
Paul
P
Duverlie
Gilles
G
Alain
Sophie
S
Chaix
Marie-Laure
ML
Gaudy
Catherine
C
Lunel
Françoise
F
Pawlotsky
Jean-Michel
JM
Payan
Christopher
C
Pozzetto
Bruno
B
Tamalet
Catherine
C
Thibault
Vincent
V
Vallet
Sophie
S
Bouchardeau
Françoise
F
Izopet
Jacques
J
Lefrère
Jean-Jacques
JJ
D016384:Consensus Sequence; D030541:Databases, Genetic; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics; D006526:Hepatitis C / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D007753:Laboratories / Q000592:standards*; D016133:Polymerase Chain Reaction; D012015:Reference Standards; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase
D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase