Effect of selenium supplementation on thyroid hormone levels and selenoenzyme activities in growing lambs.
The aim of the present study was to evaluate the effects of selenium supplementation on thyroid hormone metabolism and selenoenzyme activities in lambs. Twelve 20-d-old male lambs were assigned to one of two diets: A (0.11 ppm Se) and B (supplemented with 0.2 ppm selenium as sodium selenite). Blood samples were collected weekly for the determination of T3, T4, and selenium levels. The response to thyrotropin-releasing hormone (TRH) challenge was estimated at the 11th and 20th wk. Animals were slaughtered at wk 20 and tissues were collected for enzyme determination. Plasma selenium concentration was significantly higher in supplemented lambs (p<0.001). Plasma T3 and T4 levels remained similar in both groups. Type I deiodinase activity (ID-I) was decreased in the liver (p<0.05) and increased in the pituitary (p<0.01) of supplemented animals. No ID-I activity was detected in the thyroid. Pituitary type II deiodinase activity (ID-II) remained unchanged. The response to TRH challenge did not differ between the two groups for both challenges, but in group B, the second TRH challenge (20th wk) resulted in a significantly higher T3 response compared to the first one (11th wk) (p<0.05). In conclusion, the lack of effects of Se supplementation on thyroid hormone metabolism demonstrates that enzyme activity is homeostatically controlled and selenium is incorporated in that order to ensure the maintenance of thyroid hormone homeostasis.
Chadio
Stella E
SE
Department of Anatomy and Physiology of Farm Animals, Agricultural University of Athens, Votanikos, Athens, Greece.
Kotsampasi
Basiliki M
BM
Menegatos
John G
JG
Zervas
George P
GP
Kalogiannis
Dimitris G
DG
D000704:Analysis of Variance; D000818:Animals; D019587:Dietary Supplements; D004435:Eating; D006706:Homeostasis; D007453:Iodide Peroxidase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008297:Male; D010902:Pituitary Gland / Q000201:enzymology; D012643:Selenium / Q000008:administration & dosage* / Q000097:blood / Q000494:pharmacology; D012756:Sheep / Q000097:blood* / Q000502:physiology; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology; D013974:Thyroxine / Q000097:blood*; D013997:Time Factors; D014284:Triiodothyronine / Q000097:blood*; D015430:Weight Gain
D014284:Triiodothyronine; D013973:Thyrotropin-Releasing Hormone; D007453:Iodide Peroxidase; D012643:Selenium; D013974:Thyroxine