Cytoplasmic phospholipase A2 expression in human colon adenocarcinoma is correlated with cyclooxygenase-2 expression and contributes to prostaglandin E2 production.
Cytoplasmic phospholipase A2 (cPLA2) has a key role in prostaglandin production. The role of cPLA2 in intestinal tumorigenesis has been suggested, however, contradictory data are found in the literature. We evaluated cPLA2 and cyclooxygenase-2 (COX-2) protein expression in 65 colon carcinomas by immunohistochemistry, and in eight colorectal cancer cell lines by Western Blot. PGE2 production was evaluated by enzyme-immunoassay in the cell lines. We demonstrate that cPLA2 is overexpressed in approximately 50% of colon cancers and cell lines. cPLA2 expression is correlated with COX-2 expression. Both cPLA2 and COX-2 expressions are important in regard to PGE2 production. Our data suggest that cPLA2 might be involved in colon tumor development.
Panel
Valentine
V
INSERM, UMR S538, Paris, F-75012 France.
Boëlle
Pierre-Yves
PY
Ayala-Sanmartin
Jesus
J
Jouniaux
Anne-Marie
AM
Hamelin
Richard
R
Masliah
Joëlle
J
Trugnan
Germain
G
Fléjou
Jean-François
JF
Wendum
Dominique
D
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015153:Blotting, Western; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology; D015232:Dinoprostone / Q000096:biosynthesis*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D007150:Immunohistochemistry; D053842:Microsatellite Instability; D009367:Neoplasm Staging; D010741:Phospholipases A / Q000235:genetics* / Q000378:metabolism; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone