title: Targeted cDNA differential display (TcDD).
abstract: Targeted cDNA differential display (TcDD) was developed to study expression of a different selected gene families especially those at low copy numbers per cell. This method is an adaptation of our previously described targeted genomic differential display method (TGDD). In TcDD, the expression of genes containing target sequences such as CAG repeating sequences or genes encoding for zinc-finger binding proteins were followed in an experimental rat model with salt-induced hypertension. DNA sequencing experiments demonstrated that the effectiveness of targeting was greater than 99%.
authors.affiliation: Molecular Biology Research Laboratory and Department of Biomedical Engineering, Boston, MA 02215, USA.
authors.forename: Giang H
authors.initials: GH
authors.lastname: Nguyen
authors.forename: Nam C
authors.initials: NC
authors.lastname: Nguyen
authors.forename: Niels
authors.initials: N
authors.lastname: Storm
authors.forename: Chris
authors.initials: C
authors.lastname: Gange
authors.forename: Haralambos
authors.initials: H
authors.lastname: Gavras
authors.forename: Cassandra L
authors.initials: CL
authors.lastname: Smith
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D018390:Gene Targeting / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D012333:RNA, Messenger