Monoamine receptors in human corneal epithelium and endothelium.
OBJECTIVE
Monoamine receptors are found throughout the body. Reports about the presence of monoamine receptors in the human cornea are inconsistent.


METHODS
Immunohistochemistry, immunofluorescence and immunoblotting were used to localize monoamine receptor sites on human corneal epithelium and endothelium.


RESULTS
Antibodies to alpha-1, beta-1 and beta-2 adrenergic receptors and to D1-like and 5HT-7 receptors were bound in corneal epithelium. Antibodies to alpha-1, alpha-2A, beta-1 and beta-2 adrenergic receptors and to 5HT-7 receptors were bound in corneal endothelium.


CONCLUSIONS
Our data demonstrate the presence of several monoamine receptors in the human cornea. These receptors may play a role in the regulation of fluid transport or corneal homeostasis.
Grueb
Matthias
M
Department of Ophthalmology, University of Tuebingen, Tuebingen, Germany. matthias.grueb@med.uni-tuebingen.de
Wallenfels-Thilo
Barbara
B
Denk
Oliver
O
Mielke
Joerg
J
Reinthal
Eva
E
Rohrbach
Jens Martin
JM
Bartz-Schmidt
Karl Ulrich
KU
D000328:Adult; D000368:Aged; D015153:Blotting, Western; D004728:Endothelium, Corneal / Q000378:metabolism*; D019573:Epithelium, Corneal / Q000378:metabolism*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011941:Receptors, Adrenergic / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000378:metabolism*
D011941:Receptors, Adrenergic; D011954:Receptors, Dopamine; D011985:Receptors, Serotonin; C104425:serotonin 7 receptor