Dual wavelength imaging allows analysis of membrane fusion of influenza virus inside cells.
Influenza virus hemagglutinin (HA) is a determinant of virus infectivity. Therefore, it is important to determine whether HA of a new influenza virus, which can potentially cause pandemics, is functional against human cells. The novel imaging technique reported here allows rapid analysis of HA function by visualizing viral fusion inside cells. This imaging was designed to detect fusion changing the spectrum of the fluorescence-labeled virus. Using this imaging, we detected the fusion between a virus and a very small endosome that could not be detected previously, indicating that the imaging allows highly sensitive detection of viral fusion.
Sakai
Tatsuya
T
Department of Microbiology, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan. sakai@med.kawasaki-m.ac.jp
Ohuchi
Masanobu
M
Imai
Masaki
M
Mizuno
Takafumi
T
Kawasaki
Kazunori
K
Kuroda
Kazumichi
K
Yamashina
Shohei
S
D011992:Endosomes / Q000821:virology*; D006367:HeLa Cells; D006389:Hemagglutinins, Viral / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008561:Membrane Fusion / Q000502:physiology*; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence; D009975:Orthomyxoviridae / Q000502:physiology*; D013194:Staining and Labeling
D006389:Hemagglutinins, Viral