Strategies for evaluating B*18 allelic diversity by sequence-based typing applied to studies of a population from Singapore and African-Americans.
Strategies to resolve B*18 alleles which carry a deletion in intron 1 close to the 5' end of exon 2 relative to other HLA-B alleles or a null allele mutation in exon 1 and to resolve ambiguities among allele combinations including B*18 are described. B*18 allele frequencies from volunteer donors recruited for two hematopoietic stem cell registries show the presence of two alleles, B*180101 and B*1802, in a population from Singapore and only B*180101 in African-Americans.
Hou
L
L
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA.
Tu
B
B
Ling
G
G
Tang
T
T
Cao
K
K
Steiner
N K
NK
Lazaro
A
A
Ng
J
J
Hartzman
R J
RJ
Hurley
C K
CK
D001741:Black or African American; D000483:Alleles; D001483:Base Sequence; D002681:China / Q000208:ethnology; D017931:DNA Primers; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D015235:HLA-B Antigens / Q000235:genetics*; D059911:HLA-B18 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D007194:India / Q000208:ethnology; D008296:Malaysia / Q000208:ethnology; D008969:Molecular Sequence Data; D012042:Registries; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods*; D012846:Singapore / Q000453:epidemiology
D017931:DNA Primers; D015235:HLA-B Antigens; D059911:HLA-B18 Antigen