title: Biophysical characterization of the non-fusogenic interaction between liposomes and the shrimp bifunctional lipoprotein-beta-glucan binding protein HDL/BGBP.
abstract: Shrimp High Density Lipoprotein-beta-Glucan Binding Protein (HDL/BGBP) has been studied by its role in nutrition and innate defense. Although the mechanisms of lipid loading are still unknown, HDL-BGBP binds and aggregates phospholipids vesicles in vitro. To gain insights into the HDL-BGBP mechanism of interaction with membranes, we have used fluorescence spectroscopy and electron microscopy. Data show that HDL-BGBP does not induce membrane fusion, leakage nor lipid exchange, although microstructural changes are clearly observed. This work supports a model where protein aggregation leads to liposome clustering. Such interaction may be a critical factor for the activation of the shrimp blood cell in vivo.
authors.affiliation: Aquatic Molecular Biology Laboratory, Centro de Investigación en Alimentación y Desarrollo, A.C. PO Box 1735, Hermosillo Sonora, 83000 México.
authors.forename: Edgar F
authors.initials: EF
authors.lastname: Morán-Palacio
authors.forename: M Alejandra
authors.initials: MA
authors.lastname: Tricerri
authors.forename: Karina
authors.initials: K
authors.lastname: García-Orozco
authors.forename: Maria Gabriela
authors.initials: MG
authors.lastname: Romo-Figueroa
authors.forename: Gloria
authors.initials: G
authors.lastname: Yepiz-Plascencia
authors.forename: Horacio A
authors.initials: HA
authors.lastname: Garda
authors.forename: Rogerio R
authors.initials: RR
authors.lastname: Sotelo-Mundo
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002352:Carrier Proteins / Q000737:chemistry* / Q000648:ultrastructure; D008076:Cholesterol, HDL / Q000737:chemistry*; D003445:Crustacea; D037102:Lectins / Q000737:chemistry* / Q000648:ultrastructure; D008081:Liposomes; D008563:Membrane Lipids / Q000737:chemistry; D008854:Microscopy, Electron; D013050:Spectrometry, Fluorescence
chemical_list: D002352:Carrier Proteins; D008076:Cholesterol, HDL; D037102:Lectins; D008081:Liposomes; D008563:Membrane Lipids; C037937:glucan-binding proteins