title: Dominance relationships between self-incompatibility alleles controlled by DNA methylation.
abstract: In crucifers, the pollen S-determinant gene, SP11, is sporophytically expressed in the anther tapetum, and the pollen self-incompatibility phenotype is determined by the dominance relationships between the two S-haplotypes it carries. We report here that 5' promoter sequences of recessive SP11 alleles are specifically methylated in the tapetum before the initiation of SP11 transcription. These results suggest that tissue-specific monoallelic de novo DNA methylation is involved in determining the dominance interactions that determine the cruciferous self-incompatibility phenotype.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan. hshiba@salk.edu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiba
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kakizaki
authors.forename: Megumi
authors.initials: M
authors.lastname: Iwano
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tarutani
authors.forename: Masao
authors.initials: M
authors.lastname: Watanabe
authors.forename: Akira
authors.initials: A
authors.lastname: Isogai
authors.forename: Seiji
authors.initials: S
authors.lastname: Takayama
mesh_terms: D001937:Brassica / Q000235:genetics*; D019175:DNA Methylation; D018744:DNA, Plant / Q000235:genetics*; D005799:Genes, Dominant; D006023:Glycoproteins / Q000235:genetics*; D007178:Inbreeding; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D011058:Pollen / Q000235:genetics*; D012098:Reproduction / Q000235:genetics
chemical_list: D018744:DNA, Plant; D006023:Glycoproteins; D010940:Plant Proteins; C064156:self-incompatibility locus specific glycoprotein, Brassica