title: Effect of nitrosative stress on Schizosaccharomyces pombe: inactivation of glutathione reductase by peroxynitrite.
abstract: Oxidative stress has been shown to alter cellular redox status in various cell types. Changes in expressions of several antioxidative and antistress-responsive genes along with activation or inactivation of various proteins were also reported during oxidative insult as well as during nitrosative stress. In the present study, we show the effect of nitrosative stress on cellular redox status of fission yeast Schizosaccharomyces pombe. This is the first report of S-nitrosoglutathione (GSNO) reductase activity in S. pombe and its inactivation by GSNO. We also show the inactivation of glutathione reductase (GR) and glutathione peroxidase in the presence of various reactive nitrogen species in vivo. In addition, we first observe the inactivation of GR by peroxynitrite in vivo using S. pombe cells and also similar observations under in vitro conditions. An immunoreactive band against monoclonal anti-3-nitrotyrosine antibody confirms the modification of GR under in vitro conditions. We also show the effect of nitrosative stress on Deltapap1 cells of S. pombe, which are more sensitive to nitrosative stress, indicating the involvement of Pap1 in the protection against nitrosative stress. Finally, exposure of S. pombe cells to reactive nitrogen species reveals an important role of cellular thiol pool in protection against nitrosative stress.
authors.affiliation: Department of Biochemistry, Calcutta University, 35, Ballygunge Circular Road, Kolkata-700 019, India.
authors.forename: Rupam
authors.initials: R
authors.lastname: Sahoo
authors.forename: Tanmay
authors.initials: T
authors.lastname: Dutta
authors.forename: Amlan
authors.initials: A
authors.lastname: Das
authors.forename: Sougata
authors.initials: S
authors.lastname: Sinha Ray
authors.forename: Rajib
authors.initials: R
authors.lastname: Sengupta
authors.forename: Sanjay
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D000426:Alcohol Dehydrogenase; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D000073718:Pancreatitis-Associated Proteins; D030421:Peroxynitrous Acid / Q000494:pharmacology*; D026361:Reactive Nitrogen Species / Q000378:metabolism; D012568:Schizosaccharomyces / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D050976:Basic-Leucine Zipper Transcription Factors; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; C000612141:REG3A protein, human; D026361:Reactive Nitrogen Species; D029702:Schizosaccharomyces pombe Proteins; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; C483598:Adh5 protein, mouse; D000426:Alcohol Dehydrogenase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase