Direct detection of Cardiobacterium hominis in serum from a patient with infective endocarditis by broad-range bacterial PCR.
Bacterial DNA was detected directly in the serum of a patient with endocarditis by broad-range 16S rRNA PCR followed by sequencing and analysis of the results by the BLAST search. Using these methods, Cardiobacterium hominis was identified in 2 days from the date of serum collection. The microorganism was also isolated and identified using conventional methods (bacterial culture and biochemical tests) 17 days from the date of sample collection. This is the first report showing the direct detection of C. hominis in a patient's serum using molecular-based methods, emphasizing their potential usefulness as additional and rapid diagnostic tools for the detection and identification of fastidious bacteria.
Gatselis
N
N
Department of Medicine, Medical School, University of Thessaly, Papakiriazi 22 str, 41222 Larissa, Greece.
Malli
E
E
Papadamou
G
G
Petinaki
E
E
Dalekos
G N
GN
D044066:Cardiobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003470:Culture Media; D004269:DNA, Bacterial / Q000097:blood*; D004275:DNA, Ribosomal / Q000032:analysis; D004697:Endocarditis, Bacterial / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S