Broadband homonuclear correlation spectroscopy at high magnetic fields and MAS frequencies.
We present a new homonuclear recoupling sequence, CMAR, that allows observation of 2D 13C-13C correlation spectra at high magnetic fields and MAS frequencies (10-30 kHz). The main advantages of the sequence are that it provides efficient, broadband dipolar recoupling and concurrently decouples the 1H spins from the 13C's. Thus, no additional 1H decoupling is required during the mixing period, thereby significantly reducing the radio frequency power requirements for the experiment. Thus, CMAR significantly extends the range of applicability of the usual homonuclear recoupling techniques and should be of major interest for structure determinations of biomolecules at high magnetic fields.
De Paëpe
Gaël
G
Department of Chemistry and Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Bayro
Marvin J
MJ
Lewandowski
Józef
J
Griffin
Robert G
RG
D002247:Carbon Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009842:Oligopeptides / Q000737:chemistry
D002247:Carbon Isotopes; D009842:Oligopeptides