title: Phenotypic characterization of endometrial stromal sarcoma of the uterus.
abstract: Endometrial stromal sarcoma (ESS) of the uterus is a rare uterine malignancy that has not been characterized in detail. To characterize the phenotype of ESS of the uterus, we extracted RNA from ESS and the stroma of normal endometrium using a tissue microdissection system and compared the expression profiles in the two tissues. After suppression subtractive hybridization and differential screening, we detected the metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) gene as one of the major genes upregulated in ESS, and a full-length placental cDNA clone (CS0DI066YJ10) as one of the major genes downregulated. The results were confirmed by in situ hybridization in four resected specimens of ESS and 36 biopsy specimens of normal endometrial tissue. All ESS (4/4) and all cases of endometrial stromal cells in the proliferative phase (13/13) were positive for MALAT-1, but samples of normal stroma in the secretory phase and menopausal state included some that were negative or weakly positive for MALAT-1 (5/13 and 3/10, respectively). In contrast, all ESS and 12 of 13 cases of stromal cells in the proliferative phase were negative for the full-length placental cDNA clone but 10 of 13 cases of endometrial stromal cells in the secretory phase were positive for transcripts of the gene (P < 0.05). These results indicated that endometrial stromal cells have different phenotypic characteristics between proliferative and secretory phases and the tumor cells of ESS have the phenotypic character of endometrial stromal cells in the proliferative phase.
authors.affiliation: Department of Pathology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba-shi, Ibaraki 305-8575, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Yamada
authors.forename: Junko
authors.initials: J
authors.lastname: Kano
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsunoda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Chigusa
authors.initials: C
authors.lastname: Okubo
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ishiyama
authors.forename: Masayuki
authors.initials: M
authors.lastname: Noguchi
mesh_terms: D049109:Cell Proliferation; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D017403:In Situ Hybridization; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D012186:Retroperitoneal Neoplasms / Q000378:metabolism / Q000556:secondary; D018203:Sarcoma, Endometrial Stromal / Q000378:metabolism* / Q000473:pathology; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D013382:Subtraction Technique; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology; D014599:Uterus / Q000378:metabolism / Q000473:pathology
chemical_list: D009363:Neoplasm Proteins; D012334:RNA, Neoplasm