Structural elucidation of the core-lipid A backbone from the lipopolysaccharide of Acinetobacter radioresistens S13, an organic solvent tolerant Gram-negative bacterium.
The structure of the core oligosaccharide of the lipopolysaccharide from an organic solvent tolerant Gram-negative bacterium, Acinetobacter radioresistens S13, was investigated by chemical analysis, NMR spectroscopy and MALDI-TOF mass spectrometry. All the experiments were performed on the oligosaccharides obtained either by alkaline degradation or mild acid hydrolysis. The data showed the presence of two novel oligosaccharide molecules containing a trisaccharide of 3-deoxy-D-manno-octulopyranosonic acid in the inner core region and a glucose rich outer core whose structure is the following: [structure: see text] R=H in the main oligosaccharide and beta-Glc in the minor product. The bacterium was grown on aromatic (phenol and benzoic acid) and nonaromatic carbon sources and the core oligosaccharide resulted to occur always with this novel structure.
Leone
Serena
S
Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico II, Via Cintia, 4, I-80126 Napoli, Italy.
Molinaro
Antonio
A
Pessione
Enrica
E
Mazzoli
Roberto
R
Giunta
Carlo
C
Sturiale
Luisa
L
Garozzo
Domenico
D
Lanzetta
Rosa
R
Parrilli
Michelangelo
M
D000150:Acinetobacter / Q000737:chemistry* / Q000187:drug effects*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008050:Lipid A / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000494:pharmacology*; D012997:Solvents / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D008050:Lipid A; D009930:Organic Chemicals; D012997:Solvents