[Development of nanomedicine using intracellular DDS].
In recent years, sustained release and targeting system using nanospheres or microspheres is noticed in systemic pharmacokinetics. However, in the near future, not only "systemic pharmacokinetics" but also "intracellular pharmacokinetics" seems to be important in Drug Delivery System research. In this context, we have tried to develop the novel cytoplasmic nanoparticle (NP) delivery methods using fusogenic liposomes (FL) and protein transduction domain (PTD). In this study, we demonstrated that the FL efficiently delivered the encapsulated NP to the cytoplasm directly in a fusion -dependent. Oligonucleotides attached to NP were gradually released in the cytoplasm after its efficient delivery using FL. Furthermore, we have succeeded in identifying the novel PTD using phage displayed random peptide library. In near future, this novel PTDs are applied to cytoplasmic NP delivery carrier. From these results, we suggested that this technology is very important to control the intracellular pharmacokinetics, and can be also applied to any NP which will be produced by the nanotechnology in the future.
Yoshikawa
Tomoaki
T
Graduate School of Pharmaceutical Sciences, Osaka University.
Tsutsumi
Yasuo
Y
Nakagawa
Shinsaku
S
D003593:Cytoplasm; D016503:Drug Delivery Systems; D008081:Liposomes; D049329:Nanostructures; D036103:Nanotechnology; D019151:Peptide Library
D008081:Liposomes; D019151:Peptide Library