Aerobic biodegradation of 4-methylpyridine and 4-ethylpyridine by newly isolated Pseudonocardia sp. strain M43.
A filamentous bacterium capable of utilizing 4-methylpyridine and 4-ethylpyridine as the sole source of carbon, nitrogen and energy was isolated from sludge. The organism, designated as strain M43, clustered most closely with members of the genus Pseudonocardia by 16S rRNA gene sequence analysis. During the degradation of 4-methylpyridine and 4-ethylpyridine, c. 60% of nitrogen in the pyridine ring was released as ammonia. Metabolite analyses showed that 2-hydroxy-4-methylpyridine and 2-hydroxy-4-ethylpyridine were transiently accumulated during the degradation of 4-methylpyridine and 4-ethylpyridine, respectively. Strain M43 was also able to degrade pyridine, 3,4-dimethylpyridine, 4-carboxypyridine and 2-hydroxy-4-methylpyridine. The results indicate that degradation of 4-methylpyridine and 4-ethylpyridine by strain M43 proceeded via initial hydroxylation.
Lee
Jay J
JJ
Nakdong River Environment Research Laboratory, National Institute of Environmental Research, Kyungpook, Korea. jjlee@me.go.kr
Yoon
Jung-Hoon
JH
Yang
Sang-Yong
SY
Lee
Sung-Taik
ST
D000192:Actinomycetales / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D000332:Aerobiosis; D001420:Bacteria, Aerobic; D015373:Bacterial Typing Techniques; D001673:Biodegradation, Environmental; D003470:Culture Media; D008969:Molecular Sequence Data; D010802:Phylogeny; D010847:Picolines / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism
D003470:Culture Media; D010847:Picolines; D011725:Pyridines; D012722:Sewage; D014874:Water Pollutants, Chemical