New TEM-derived extended-spectrum beta-lactamase and its genomic context in plasmids from Salmonella enterica serovar derby isolates from Uruguay.
A small (8.2-kb) ColE1 plasmid encoding TEM-144 (a new beta-lactamase with a ceftazidimase profile) was sequenced by a gene-walking strategy. The bla(TEM) allele was carried on a Tn2 element, disrupting a Rom protein gene. TEM-144 differs from TEM-1 by two mutations (R164C and E240K) and from the ceftazidime-hydrolyzing TEM-91 by one mutation (T182M).
Vignoli
Rafael
R
Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, 11600 Montevideo, Uruguay.
Cordeiro
Nicolas F
NF
García
Virginia
V
Mota
María Inés
MI
Betancor
Laura
L
Power
Pablo
P
Chabalgoity
José A
JA
Schelotto
Felipe
F
Gutkind
Gabriel
G
Ayala
Juan A
JA
D001483:Base Sequence; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D010957:Plasmids; D019779:Salmonella enterica / Q000201:enzymology* / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
D004251:DNA Transposable Elements; D001618:beta-Lactamases