Murine fetal small-intestine grafts: morphometric and immunohistochemical evaluation.
We investigated histopathological changes following murine fetal intestinal transplantation. Fetal intestine, obtained from a pregnant C57BL/6 mouse, was transplanted into BALB/c and C57Bl/6 mice. Recipients were divided into three groups: isogeneic, and allogeneic treated with 3 mg/kg/day gangliosides (Allo-a) or 9 mg/kg/day (Allo-b). One week after transplant, all grafts showed good viability, confirmed by cellular mitosis in the mucosa and a well-defined propria muscular layer. Isogeneic grafts showed a thicker muscular layer than in the Allo-a (P = 0.02) and Allo-b (P = 0.004) groups. There was no difference in number of mitotic cells among groups. Goblet cells were significantly reduced in allografts treated with 3 mg gangliosides (P = 0.013) or 9 mg gangliosides (P = 0.002) compared to isografts. Villi height was similar in all studied groups. There was no difference in positivity of the enteric nervous system among groups. Atrophy was more common in the allogeneic groups, suggesting that isografts had better development than allografts treated with gangliosides. (
de Almeida
Carlos Eduardo Saldanha
CE
Department of Surgery, Universidade Federal de São Paulo, São Paulo, Brazil.
de Oliveira
Kátia Ferreira
KF
Manna
Mônica Cecília Bochetti
MC
Artigiani
Ricardo
R
Koike
Márcia Kiyomi
MK
Montero
Edna Frasson de Souza
EF
D000818:Animals; D005260:Female; D016332:Fetal Tissue Transplantation / Q000276:immunology / Q000473:pathology*; D005732:Gangliosides / Q000502:physiology*; D006085:Graft Survival; D007421:Intestine, Small / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009418:S100 Proteins / Q000378:metabolism; D014184:Transplantation, Homologous
D005732:Gangliosides; D009418:S100 Proteins