Thorsellia anophelis gen. nov., sp. nov., a new member of the Gammaproteobacteria.
A Gram-negative, rod-shaped organism (CCUG 49520T) was isolated from the midgut of the mosquito Anopheles arabiensis. 16S rRNA gene sequence analysis demonstrated that this isolate is unique, showing <93% similarity to species of the families Enterobacteriaceae and Vibrionaceae. The quinone system consisted exclusively of ubiquinone Q-8; the polar lipid profile consisted of the major compounds phosphatidylethanolamine and phosphatidylglycerol, a moderate to minor amount of two unknown aminophospholipids, an unknown phospholipid and two unknown polar lipids; the polyamine pattern was characterized by the predominant compound 1,3-diaminopropane and showed some significant differences when compared with members of the Enterobacteriaceae and Vibrionaceae. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic data, strain CCUG 49520T is considered to represent a new genus and species, for which the name Thorsellia anophelis gen. nov., sp. nov. is proposed. The type strain is CCUG 49520T (=CIP 108754T).
Kämpfer
Peter
P
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, IFZ-Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
Lindh
Jenny M
JM
Department of Genetics, Microbiology and Toxicology, S-106 91 Stockholm University, Stockholm, Sweden.
Terenius
Olle
O
Department of Genetics, Microbiology and Toxicology, S-106 91 Stockholm University, Stockholm, Sweden.
Haghdoost
Siamak
S
Department of Genetics, Microbiology and Toxicology, S-106 91 Stockholm University, Stockholm, Sweden.
Falsen
Enevold
E
CCUG, Culture Collection University of Göteborg, Göteborg, Sweden.
Busse
Hans-Jürgen
HJ
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, Wien, Austria.
Faye
Ingrid
I
Department of Genetics, Microbiology and Toxicology, S-106 91 Stockholm University, Stockholm, Sweden.
D000818:Animals; D000852:Anopheles / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004064:Digestive System / Q000382:microbiology; D004755:Enterobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology; D020563:Gammaproteobacteria / Q000145:classification*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S