[HLA-A, B, DRB1 gene polymorphism of Beijing population was studied by high-resolution polymerase chain reaction sequence-based typing].
OBJECTIVE
To investigate the gene polymorphism of human leukocyte antigen (HLA)-A, B, DRB1 loci in the population of Beijing region, and research on the application feasibility of polymerase chain reaction sequence-based typing (PCR-SBT) method.


METHODS
PCR-SBT method was applied to determine HLA- A, B, DRB1 genotypes of 618 unrelated healthy individuals of Beijing region.


RESULTS
A total of 84 different alleles and 199 genotypes of HLA-A, 143 alleles and 366 genotypes of HLA-B, 122 alleles and 286 genotypes of HLA-DRB1 were detected.


CONCLUSIONS
The results showed the characteristics of HLA-A, B, DRB1 distributions, and provided more comprehensive and accurate gene data that may serve as normal reference values for all of Beijing people.
Deng
Ya-jun
YJ
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, P.R.China.
Yang
Guang
G
Wu
Dong-ying
DY
Hu
Song-nian
SN
Li
Sheng-bin
SB
Zhu
Jun
J
Zhu
Bo-feng
BF
Liu
Yao
Y
D044466:Asian People / Q000235:genetics*; D002681:China / Q000208:ethnology; D005260:Female; D005828:Genetics, Population; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011153:Population
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains