Antioxidative function and biodistribution of [Gd@C82(OH)22]n nanoparticles in tumor-bearing mice.
Oxidative stress is considered to be one of the important mechanisms involved in carcinogenesis. In our previous study, gadolinium endohedral metallofullerenol ([Gd@C82(OH)22]n nanoparticles) have shown high inhibitory activity on hepatoma cell (H22) growth in mice. To explore the antioxidative functions of nanoparticles, we investigated the biodistribution of [Gd@C82(OH)22]n nanoparticles, the changes of blood coagulation profiles, the metabolism of reactive oxygen species (ROS) in the tumor-bearing mice as well as the possible relationships between nanoparticles treatment and ROS production in this paper. The activities of hepatic superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) and catalase (CAT) as well as the levels of reduced glutathione (GSH), protein-bound thiols and malondialdehyde (MDA) were compared between the tumor-bearing mice and normal mice. Transplanted tumors were grown in mice by subcutaneous injection of murine hepatoma cells in the mice. The comparison of the above parameters between nanoparticles and cyclophosphamide (CTX) therapy were also investigated. [Gd@C82(OH)22]n administration can efficiently restore the damaged liver and kidney of the tumor-bearing mice. All the activities of enzymes and other parameters related to oxidative stress were reduced after [Gd@C82(OH)22]n treatment and tended closely to the normal levels. The results suggest that [Gd@C82(OH)22]n nanoparticle treatment could regulate ROS production in vivo.
Wang
Jiangxue
J
Lab for Bio-Environmental Health Sciences of Nanoscale Materials and Key Lab of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, P.O. Box 918, Beijing 100049, PR China.
Chen
Chunying
C
Li
Bai
B
Yu
Hongwei
H
Zhao
Yuliang
Y
Sun
Jin
J
Li
Yufeng
Y
Xing
Gengmei
G
Yuan
Hui
H
Tang
Jun
J
Chen
Zhen
Z
Meng
Huan
H
Gao
Yuxi
Y
Ye
Chang
C
Chai
Zhifang
Z
Zhu
Chuanfeng
C
Ma
Baocheng
B
Fang
Xiaohong
X
Wan
Lijun
L
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000493:pharmacokinetics* / Q000627:therapeutic use; D001777:Blood Coagulation / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology; D005260:Female; D037741:Fullerenes / Q000737:chemistry / Q000493:pharmacokinetics* / Q000627:therapeutic use; D005682:Gadolinium / Q000493:pharmacokinetics* / Q000627:therapeutic use; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D049329:Nanostructures; D036103:Nanotechnology / Q000379:methods; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D009942:Organometallic Compounds / Q000737:chemistry / Q000493:pharmacokinetics* / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects; D014018:Tissue Distribution; D023041:Xenograft Model Antitumor Assays
D000975:Antioxidants; D037741:Fullerenes; D009942:Organometallic Compounds; D005682:Gadolinium