[Expression of IgVH and B7-1 in proteome of the human colorectal carcinoma cell lines].
OBJECTIVE
To conduct a proteomic analysis of human colorectal carcinoma cell lines LS174T and SW480 by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).


METHODS
The total proteins of human colorectal carcinoma cell lines LS174T and SW480 were separated with 2-DE using immobilized pH gradient strips and analyzed by MALDI-TOF-MS to obtain peptide mass fingerprints (PMFs). Proteins were identified by using Mascot software to search protein databases.


RESULTS
Good resolution 2-DE maps were obtained. Some proteins including immunoglobulin heavy chain variable region (IgVH) and co-stimulatory molecule B7-1 were identified. IgVH and B7-1 were confirmed by electrospray ionization tandem spectrometry (ESI-MS/MS) and immunocytochemistry.


CONCLUSIONS
There are IgVH and B7-1 expressions in human colorectal carcinoma cell lines LS174T and SW480. Results obtained will help to elucidate the mechanisms of tumor immune escape.
Zhang
Ying-tao
YT
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Cancer Research, School of Life Science & Technology, Xi'an Jiaotong University, Xi'an 710061, China.
Geng
Yi-ping
YP
Lai
Bao-chang
BC
Si
Lü-sheng
LS
Wang
Yi-li
YL
D018122:B7-1 Antigen / Q000096:biosynthesis* / Q000235:genetics; D002461:Cell Line, Transformed; D015179:Colorectal Neoplasms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis* / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D010449:Peptide Mapping; D020543:Proteome / Q000302:isolation & purification
D018122:B7-1 Antigen; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D009363:Neoplasm Proteins; D020543:Proteome