N-t-Butyl hydroxylamine regulates heat shock-induced apoptosis in U937 cells.
Heat shock may increase oxidative stress due to increased production of reactive oxygen species and/or the promotion of cellular oxidation events. Therefore, compounds that scavenge reactive oxygen species may regulate heat shock-induced cell death. Recently, it has been shown that the decomposition product of the spin-trapping agent alpha-phenyl-N-t-butylnitrone, N-t-butyl hydroxylamine (NtBHA), mimics alpha-phenyl-N-t-butylnitrone and is much more potent in delaying reactive oxygen species-associated senescence. We investigated the protective role of NtBHA against heat shock-induced apoptosis in U937 cells. Upon exposure to heat shock, there was a distinct difference between the untreated cells and the cells pre-treated with 0.1 mM NtBHA for 2 h in regard to apoptotic parameters, cellular redox status, and mitochondrial function. Upon exposure to heat shock, NtBHA pre-treated cells showed significant inhibition of apoptotic features such as activation of caspase-3, up-regulation of Bax, and down-regulation of Bcl-2 compared to untreated cells. This study indicates that NtBHA may play an important role in regulating the apoptosis induced by heat shock, presumably through scavenging of reactive oxygen species.
Kim
Hyun Jeong
HJ
School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu, Korea.
Shin
Seoung Woo
SW
Oh
Chang Joo
CJ
Lee
Mann Hyung
MH
Yang
Chae Ha
CH
Park
Jeen-Woo
JW
D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006358:Hot Temperature; D006801:Humans; D006898:Hydroxylamines / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013997:Time Factors; D020298:U937 Cells
D006898:Hydroxylamines; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D013113:Spin Labels; C072771:N-tert-butylhydroxylamine; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases