Evaluating outlier loci and their effect on the identification of pedigree errors.
Homozygosity outlier loci, which show patterns of variation that are extremely divergent from the rest of the genome, can be evaluated by comparison of the homozygosity under Hardy-Weinberg proportions (the sum of the squares of allele frequencies) with the expected homozygosity under neutrality. Such outlier loci are potentially under selection (balancing selection or directional selection) when genome-wide effects (such as bottleneck and rapid population growth) are excluded. Outlier loci show skewed allele frequencies with respect to neutrality and may therefore affect the identification of pedigree errors. However, choosing neutral markers (excluding outlier loci) for the identification of pedigree errors has been neglected thus far. Our results showed that 4.1%, 5.5%, and 1.5% of the microsatellite markers, Illumina single-nucleotide polymorphisms (SNPs), and Affymetrix SNPs, respectively, on the autosomes appear to be under balancing selection (p <or= 0.01) while 0.8% of the Affymetrix SNPs are consistent with directional selection. On the X-chromosome, 7.7%, 3.2%, and 0.4% of the microsatellite markers, Illumina SNPs, and Affymetrix SNPs, respectively, appear to be under balancing selection. 9.3% of Illumina SNPs and 6.7% of Affymetrix SNPs which have high minor allele frequency (>or=40%) appear to be under balancing selection. Pedigree structure errors in 15 of 143 pedigrees were detected using microsatellite markers from the autosomes and/or selected SNPs from chromosomes 1 to 18 of the Illumina and/or selected SNPs from chromosomes 1 to 16 of the Affymetrix. Outlier loci did not make a major difference to the identification of pedigree errors. The Collaborative Study on the Genetics of Alcoholism data has pedigree errors and some of them may be due to sample mix up.
Wang
Ke-Sheng
KS
Program in Genetics and Genomic Biology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada. ke-sheng.wang@sickkids.ca
Liu
Michelle
M
Paterson
Andrew D
AD
D000437:Alcoholism / Q000235:genetics; D003299:Cooperative Behavior; D030541:Databases, Genetic; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D010375:Pedigree; D012107:Research Design