Degradation of high-molecular-weight hyaluronan by hydrogen peroxide in the presence of cupric ions.
Dynamic viscosity (eta) of the high-molecular-weight hyaluronan (HA) solution was measured by a Brookfield rotational viscometer equipped with a Teflon cup and spindle of coaxial cylindrical geometry. The decrease of eta of the HA solution, indicating degradation of the biopolymer, was induced by a system containing H2O2 alone or H2O2 plus CuCl2. The reaction system H2O2 plus CuCl2 as investigated by EPR spin-trapping technique revealed the formation of a four-line EPR signal characteristic of a *DMPO-OH spin adduct. Thus, hydroxyl radicals are implicated in degradation of high-molecular-weight HA by the system containing H2O2 and CuCl2.
Soltés
Ladislav
L
Institute of Experimental Pharmacology, Slovak Academy of Sciences, SK-84104 Bratislava, Slovak Republic. ladislav.soltes@savba.sk
Brezová
Vlasta
V
Stankovská
Monika
M
Kogan
Grigorij
G
Gemeiner
Peter
P
D003300:Copper / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D006820:Hyaluronic Acid / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D008970:Molecular Weight
D003300:Copper; D006820:Hyaluronic Acid; D006861:Hydrogen Peroxide; C029892:cupric chloride