Cell refractive index tomography by digital holographic microscopy.
For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.
Charrière
Florian
F
Ecole Polytechnique Fédérale de Lausanne, Imaging and Applied Optics Institute, Switzerland. florian.charriere@epfl.ch
Marian
Anca
A
Montfort
Frédéric
F
Kuehn
Jonas
J
Colomb
Tristan
T
Cuche
Etienne
E
Marquet
Pierre
P
Depeursinge
Christian
C
D000465:Algorithms; D006696:Holography / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D011058:Pollen / Q000648:ultrastructure*; D012031:Refractometry / Q000379:methods*; D012815:Signal Processing, Computer-Assisted; D041622:Tomography, Optical / Q000379:methods*