title: The three-dimensional folding of the tRNA-like structure of tobacco mosaic virus RNA. A new building principle applied twice.
abstract: The structure of the tRNA-like 3' terminus of tobacco mosaic virus (TMV) RNA has been studied. A 3' -terminal fragment possessing the tRNA-like properties was probed with chemical modification and enzymatic digestions. A model of the secondary structure is proposed for the last 105 nucleotides. The corresponding region of other tobamoviral RNAs can be folded in an identical secondary structure. A three-dimensional model for the tRNA-like structure is given which is compared with those proposed earlier for the tRNA-like 3' termini of turnip yellow mosaic virus (TYMV) RNA and brome mosaic virus (BMV) RNA. A new building principle which we discovered previously by studying the latter RNAs appears to be applied twice in the tRNA-like structure of TMV RNA. The determination of the minimal length requirement for recognition of CTP, ATP:tRNA nucleotidyl-transferase reveals a size of 100 nucleotides in agreement with the models proposed.
authors.affiliation: Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands.
authors.forename: K
authors.initials: K
authors.lastname: Rietveld
authors.forename: K
authors.initials: K
authors.lastname: Linschooten
authors.forename: C W
authors.initials: CW
authors.lastname: Pleij
authors.forename: L
authors.initials: L
authors.lastname: Bosch