Gold-nanoparticle-assisted oligonucleotide immobilisation for improved DNA detection.
Colloidal gold nanoparticles are investigated as a potential scaffold for the assisted immobilisation of probe oligonucleotides on silicon surfaces. A preliminary study is devoted to the examination of the immobilisation of DNA-modified gold nanoparticles as a function of time, concentration, salt and pH. The DNA-modified nanoparticles self-assembled onto solid surfaces in a three-dimensional self-assembled architecture. The functionalised surfaces are evaluated in diagnostic assays, where their potential to improve the efficiency of the hybridisation reaction is tested. The system utilising DNA-modified nanoparticles produced an enhancement in the hybridisation efficiency and the sensitivity limit by a factor 10 to 100 as compared to a conventional DNA immobilisation system on a planar surface.
Minard-Basquin
C
C
Stuttgart Technology Center, Materials Science Laboratory, Sony Deutscheland (GmbH), D-70327 Stuttgart, Germany. Basquin@sony.de
Kügler
R
R
Matsuzawa
N N
NN
Yasuda
A
A
D000327:Adsorption; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D020099:Coated Materials, Biocompatible; D006046:Gold; D004247:DNA