title: Characterization of interstitial cells of Cajal in bowel of cattle (Bos taurus).
abstract: Interstitial cells of Cajal (ICC) have been described in the gastrointestinal tract of different mammals including humans, horses, pigs, rats, dogs, mice and guinea-pigs. In the present study, ICC were identified in the jejunum of Bos taurus using polyclonal anti-c-Kit antibodies in immunohistochemical assays. Vimentin and desmin intermediate filaments were also determined using monoclonal antibodies. ICC were found in the tunica muscularis either in a palisade distribution pattern between the outer longitudinal and the inner circular layers (ICC-MP) or freely distributed in clusters in the longitudinal layer (ICC-LM). Morphometric studies determined that ICC have a fusiform shape presenting cytoplasmic prolongations. ICC were positive to c-Kit and vimentin antigens but negative to desmin. We have observed and described for the first time the presence of ICC in a ruminant. As observed in the aforementioned mammals, bovine ICC were associated with the myenteric plexus. Nevertheless, the presence of widespread ICC in the longitudinal muscular layer of the jejunum differs from previously described studies of other mammals.
authors.affiliation: Ciclo Bá sico Común, Departamento de Biología, Universidad de Buenos Aires, Buenos Aires.
authors.forename: S G
authors.initials: SG
authors.lastname: Márquez
authors.forename: J M
authors.initials: JM
authors.lastname: Galotta
authors.forename: E L
authors.initials: EL
authors.lastname: Portiansky
authors.forename: C G
authors.initials: CG
authors.lastname: Barbeito
mesh_terms: D000818:Animals; D000906:Antibodies; D002417:Cattle / Q000033:anatomy & histology*; D003893:Desmin / Q000378:metabolism; D005260:Female; D007583:Jejunum / Q000166:cytology*; D009197:Myenteric Plexus; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D000906:Antibodies; D003893:Desmin; D014746:Vimentin; D019009:Proto-Oncogene Proteins c-kit