Identification of a novel HLA-A allele (A*1115) in the UK National External Quality Assessment Schemes for Histocompatibility and Immunogenetics' Educational Cell Exchange.
The novel allele, HLA-A*1115, was identified in an 'Educational Scheme' sample (ED03/03 - from a north-western European Caucasoid blood donor) distributed by the UK National External Quality Assessment Schemes for Histocompatibility and Immunogenetics. ED03/03 was typed by serology, the polymerase chain reaction using sequence-specific primers and sequence-based typing. A*1115 is most similar to A*110101 with a single mismatch (G to C) at constant position 565, leading to a conservative amino acid change from valine (GTG) to leucine (CTG) at codon 165 in the alpha(2) domain. This substitution has not been reported for any other HLA class I allele so far. The HLA-A*1115-bearing haplotype was B*350101; Cw*040101; DRB1*140101; DRB3*020201; DQA1*010401; DQB1*0503; DPA1*0103/07; DPB1*030101. Extensive serological typing indicated that this allele essentially encodes a 'normal' HLA-A11 specificity.
Bendukidze
N
N
H&I Department, National Blood Service, Bristol, UK. nina.bendukidze@nhs.nbs.uk
Day
S
S
Street
J
J
Hammond
L
L
Downing
J
J
Corbin
S
S
Dunn
P P J
PP
Darke
C
C
D000483:Alleles; D001483:Base Sequence; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D015234:HLA-A Antigens / Q000235:genetics*; D006239:Haplotypes; D006648:Histocompatibility; D006801:Humans; D007125:Immunogenetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D006113:United Kingdom; D044465:White People
D015234:HLA-A Antigens