title: Fast mass spectrometry-based methodologies for pharmaceutical analyses.
abstract: Historically, most bioanalytical methods for drug analysis in pharmaceutical industry were developed using HPLC coupled with UV or fluorescence detection. However, there is a trend toward interfacing separation technologies with more sensitive tandem mass spectrometry (MS/MS)-based systems. MS/MS detection offers complete resolution of the parent compounds from their first pass metabolites to avoid extra efforts for separation and sample clean-up procedures resulting in shorter run times. With the increasing demand for ever faster screening, there is a continuing demand for bioanalytical methods possessing higher sample throughput for both in vitro and in vivo drug metabolism and pharmacokinetic evaluations to accelerate the discovery process. This review focuses on the current approaches for fast MS-based assays (cycle-time less than 5 min) of pharmaceuticals and their metabolites that have been reported in the peer-reviewed publications.
authors.affiliation: Department of Drug Metabolism and Pharmacokinetics, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA. yunsheng.hsieh@spcorp.com
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Hsieh
authors.forename: Elaine
authors.initials: E
authors.lastname: Fukuda
authors.forename: Julie
authors.initials: J
authors.lastname: Wingate
authors.forename: Walter A
authors.initials: WA
authors.lastname: Korfmacher
mesh_terms: D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013997:Time Factors
chemical_list: D004364:Pharmaceutical Preparations