title: Digital holographic microscopy with dual-wavelength phase unwrapping.
abstract: We apply the techniques of digital holography to obtain microscopic three-dimensional images of biological cells. The optical system is capable of microscopic holography with diffraction-limited resolution by projecting a magnified image of a microscopic hologram plane onto a CCD plane. Two-wavelength phase-imaging digital holography is applied to produce unwrapped phase images of biological cells. The method of three-wavelength phase imaging is proposed to extend the axial range and reduce the effect of phase noise. These results demonstrate the effectiveness of digital holography in high-resolution biological microscopy.
authors.affiliation: Department of Physics, University of South Florida, Tampa 33620, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Parshall
authors.forename: Myung K
authors.initials: MK
authors.lastname: Kim
mesh_terms: D000465:Algorithms; D006696:Holography / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D019697:Onions / Q000166:cytology*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted