Highbrow proteasome in high-throughput technology.
Proteasome is a major protease of the ubiquitin-proteasome pathway involved in the regulation of practically all intracellular biochemical processes. The enzyme core is created by a heteromultimer of complex architecture built with multiple subunits arranged into a tube-like structure. The multiple active sites of diverse peptidase specificity are hidden inside the tube. Access to the interior is guarded by a gate formed by the N-termini of specialized subunits and by the attachment of additional multisubunit protein complexes controlling the enzymatic capabilities of the core. Proteasome, due to its Byzantine molecular architecture and equally sophisticated enzymatic mechanism, is by itself a fascinating biophysical object. Recently, the position of the protease advanced from an academically remarkable protein processor to a providential anticancer drug target and futuristic nanomachine. Proteomics studies actively shape our current understanding of the protease and direct the future applications of the proteasome in medicine.
Gaczynska
Maria
M
Institute of Biotechnology, Department of Molecular Medicine, The University of Texas Health Science Center, San Antonio, TX 78245, USA. gaczynska@uthscsa.edu
Rodriguez
Karl
K
Madabhushi
Srividya
S
Osmulski
Pawel A
PA
D000494:Allosteric Regulation / Q000235:genetics; D000818:Animals; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry* / Q000378:metabolism*; D061988:Proteasome Inhibitors; D025801:Ubiquitin / Q000378:metabolism
D061988:Proteasome Inhibitors; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex