Blue eye disease porcine rubulavirus (PoRv) infects pig neurons and glial cells using sialo-glycoprotein as receptor.
Pig neural cells express glycoproteins with sialylated N-linked oligosaccharide chains (SNOC) which are used by the porcine rubulavirus (PoRv) as receptors. Pig neuronal or glial cell cultures were employed to investigate (a) whether PoRv infects such cells using a molecule expressing SNOC, and (b) the role of viral envelope glycoproteins in establishing the infection. Enriched neuronal or glial cell cultures were exposed to PoRv and infection was detected immunocytochemically. Neuronal cultures prepared from neonatal pigs were treated enzymatically to eliminate sialic acid or N-linked oligosaccharide chains. Primary neural cultures were exposed to anti-HN or anti-F preincubated with PoRv to study the role of the viral glycoproteins. In enriched cultures, PoRv infected neurons and glial cells, and sialic acid expressed in N-linked oligosaccharide chains appeared to play a central role in infection. It was concluded that HN and F viral glycoproteins are required to infect neurons and glial cells.
Mendoza-Magaña
Maria L
ML
Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Guadalajara, Jalisco, Mexico. mmendoza@cucs.udg.mx
Godoy-Martinez
Diana V
DV
Guerrero-Cazares
Hugo
H
Rodriguez-Peredo
Alejandra
A
Dueñas-Jimenez
Judith M
JM
Dueñas-Jiménez
Sergio H
SH
Ramírez-Herrera
Mario A
MA
D000818:Animals; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D009439:Neuraminidase / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism; D019213:Rubulavirus / Q000502:physiology*; D019351:Rubulavirus Infections / Q000662:veterinary* / Q000821:virology; D012795:Sialoglycoproteins / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000821:virology; D014758:Viral Core Proteins / Q000378:metabolism
C039111:HN protein receptor; D011991:Receptors, Virus; D012795:Sialoglycoproteins; D014758:Viral Core Proteins; D009439:Neuraminidase; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase