Species-specific restriction of cell surface expression of mouse MARCO glycoprotein in murine cell lines.
The MARCO (macrophage receptor with collagenous structure) glycoprotein belongs to the scavenger receptor type family of pattern-recognition molecules produced by a subset of marginal zone macrophages in the spleen. Stimulation with LPS leads to its appearance on macrophages located at other tissue compartments. In the present work, we report its in vitro expression by various cell lines using transient and stable (lentiviral) gene delivery aimed at investigating the signaling properties of this receptor and its analysis using a novel rat monoclonal antibody against the SRCR-domain of mouse MARCO. When trying to establish stable mouse MARCO-transfectants using lentiviral transduction and other methods, we consistently found that MARCO accumulated intracellularly in various murine host cells. In contrast, such a phenomenon was not observed in non-murine cell lines. Our observations indicate the presence of an unexpected limitation of the in vitro expression of mouse MARCO glycoprotein in murine cell lines. We believe that the failure to express MARCO on the cell surface of the many murine cell lines is likely due to the absence of endoplasmic reticulum molecular chaperones needed for the correct folding and assembly of the trimeric MARCO molecule.
Kvell
Krisztián
K
Department of Immunology and Biotechnology, Faculty of Medicine, University of Pécs, Szigeti út 12, H-7634 Pécs, Hungary.
Czömpöly
Tamás
T
Pikkarainen
Timo
T
Balogh
Péter
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D000939:Epitopes; D005260:Female; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D006801:Humans; D019169:Jurkat Cells; D016086:Lentivirus / Q000235:genetics; D008264:Macrophages / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011487:Protein Conformation; D051381:Rats; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000235:genetics; D013045:Species Specificity; D014162:Transfection
D000911:Antibodies, Monoclonal; D000939:Epitopes; C092228:Marco protein, mouse; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic