title: Change in immunoreactive human hepatic triglyceride lipase (HTGL) mass and the shelf-life of the HTGL ELISA kit in long-term storage.
abstract: Objectives of this work are to study changes in the immunoreactive HTGL mass during storage under various conditions. In addition, the shelf-life of the HTGL ELISA kit was confirmed. The immunological reactivity of HTGL in PHP stored in the liquid, frozen, or lyophilized state was monitored using purified human PHP-HTGL as the standard material. Furthermore, the long-term stability of the HTGL ELISA kit was ascertained. The immunoreactive HTGL mass in the lyophilized PHP maintained its initial immunological reactivity for at least 26 months at 4 degrees C or lower. The other reagents included in the HTGL ELISA kit also have a long shelf-life when they are stored at 4 degrees C or less. HTGL in PHP was stabilized by lyophilization and can be used as the standard material for HTGL ELISA; the HTGL ELISA kit has a long shelf-life, i.e., more than two years.
authors.affiliation: Division of Laboratory Products, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan. makoto-nishimura@dainippon-pharm.co.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishimura
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Iwanaga
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Ohkaru
authors.forename: Atsuko
authors.initials: A
authors.lastname: Takagi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ikeda
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D004355:Drug Stability; D004356:Drug Storage; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005612:Freeze Drying; D006801:Humans; D008049:Lipase / Q000032:analysis* / Q000276:immunology / Q000592:standards; D008099:Liver / Q000201:enzymology; D011933:Reagent Kits, Diagnostic; D013696:Temperature; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; C504173:LIPC protein, human; D011933:Reagent Kits, Diagnostic; D008049:Lipase