[Virulent properties of hospital strains of bacteria of the Burkholderia cepacia complex, isolated in hospitals of Moscow].
The results of the study of hospital strains of the B. cepacia complex, isolated in hospitals of Moscow, with the use of phenotypical and molecular-genetic methods are presented. The phenotypical methods made it possible to differentiate Russian strains and classify them with a group of genomovars (I, III, IV). As the result the epidemic importance of the strains with epidemic markers, having specific characteristics for every clinic, was determined. The detection of the collection of genes cepI and cepR in the strains made confirmed the epidemic importance of the stains which had, due to the regulatory "quorum sensing" (QS) system, the potential capacity for inducing infection and persisting in the patient's body. The presence of gene cepR in all strains and the absence of gene cepl in 33% of strains gave evidence to suggest that in some strains the activation of the production of pathogenicity factors required the presence of other bacteria having the fully developed QS system. Thus, the new complex approach with the use of phenotypical and molecular-genetic methods permits more precise identification of the source of hospital infection induced by the bacteria of the B. cepacia complex.
Chernukha
M Iu
MIu
Alekseeva
G V
GV
Shaginian
I A
IA
Romanova
Iu M
IuM
Stepanova
T V
TV
Batov
A B
AB
Gintsburg
A L
AL
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D015415:Biomarkers; D019121:Burkholderia Infections / Q000382:microbiology* / Q000635:transmission; D016956:Burkholderia cepacia / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D003428:Cross Infection / Q000382:microbiology*; D005798:Genes, Bacterial / Q000235:genetics; D006786:Hospitals, Urban; D006801:Humans; D008025:Ligases / Q000235:genetics; D009031:Moscow; D013045:Species Specificity; D014774:Virulence
D001426:Bacterial Proteins; D015415:Biomarkers; C117246:CepR protein, Burkholderia; D008025:Ligases; C449816:N-acylhomoserine lactone synthase