Evaluation of fusion between liposomes and erythrocytes for intracellular derivatization of amino acids in cells.
Erythrocytes were fused with liposome for intracellular derivatization of amino acids in cells. The fusion efficiency was evaluated with capillary electrophoresis (CE) and laser-induced fluorescence (LIF) detection. Reagent fluorescein isothiocyanate (FITC) was enveloped in liposomes and introduced into erythrocytes by fusion between liposomes and erythrocytes. The amino acids in the fused cells were derivated by the introduced FITC and the derivated amino acids were extracted for detection by capillary electrophoresis equipped with laser-induced fluorescence detector. The fusion conditions were investigated. It was found that incubation of liposome and erythrocytes in the presence of 13% polyethylene glycol 6000 (PEG 6000) for 15min produced the highest fusion efficiency and kept the erythrocytes stability.
Zhang
Hua
H
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
Du
Juan
J
Zhao
Jie
J
Li
Yue-zhong
YZ
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes; D007834:Lasers; D008081:Liposomes / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D000596:Amino Acids; D005456:Fluorescent Dyes; D008081:Liposomes; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate