title: The Arg279Gln [corrected] substitution in the adenovirus type 11p (Ad11p) fiber knob abolishes EDTA-resistant binding to A549 and CHO-CD46 cells, converting the phenotype to that of Ad7p.
abstract: The major determinant of adenovirus (Ad) attachment to host cells is the C-terminal knob domain of the trimeric fiber protein. Ad type 11p (Ad11p; species B2) in contrast to Ad7p (species B1) utilizes at least two different cellular attachment receptors, designated sBAR (species B adenovirus receptor) and sB2AR (species B2 adenovirus receptor). CD46 has recently been identified as one of the Ad11p attachment receptors. However, CD46 did not seem to constitute a functional receptor for Ad7p. Although Ad7p shares high knob amino acid identity with Ad11p, Ad7p is deficient in binding to both sB2AR and CD46. To determine what regions of the Ad11p fiber knob are necessary for sB2AR-CD46 interaction, we constructed recombinant fiber knobs (rFK) with Ad11p/Ad7p chimeras and Ad11p sequences having a single amino acid substitution from Ad7p. Binding of the constructs to A549 and CHO-CD46 BC1 isoform-expressing cells was analyzed by flow cytometry. Our results indicate that an Arg279Gln [corrected] substitution is sufficient to convert the Ad11p receptor-interaction phenotype to that of Ad7p and abolish sB2AR and CD46 interaction. Also a Glu279Arg substitution in Ad7p rFKs increases CD46 binding. Thus, the lateral HI loop of the Ad11p fiber knob seems to be the key determinant for Ad11p sB2AR-CD46 interaction. This result is comparable to another non-coxsackie-adenovirus receptor binding Ad (Ad37p), where substitution of one amino acid abolishes virus-cell interaction. In conjunction with previous results, our findings also strongly suggest that sB2AR is equivalent to CD46.
authors.affiliation: Department of Virology, Umeå University, SE-901 85 Umeå, Sweden. dan.gustafsson@climi.umu.se
authors.forename: Dan J
authors.initials: DJ
authors.lastname: Gustafsson
authors.forename: Anna
authors.initials: A
authors.lastname: Segerman
authors.forename: Kristina
authors.initials: K
authors.lastname: Lindman
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Mei
authors.forename: Göran
authors.initials: G
authors.lastname: Wadell
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics / Q000254:growth & development*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D016466:CHO Cells; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D007150:Immunohistochemistry; D051916:Membrane Cofactor Protein / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D011487:Protein Conformation; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D011991:Receptors, Virus / Q000378:metabolism*; D011995:Recombination, Genetic
chemical_list: D036022:Capsid Proteins; D051916:Membrane Cofactor Protein; D011991:Receptors, Virus; C018315:hexon capsid protein, Adenovirus