Chiral separation and identification of beta-aminoketones of pharmacological interest by high performance liquid chromatography and capillary electrophoresis.
This paper describes the development and comparison of chiral methods of analysis for a series of pharmacologically active indane derivatives that have been studied in the context of the evaluation of a promising prodrug system for amines. The methods are intended for studying the differences in the pharmacokinetics of the optical isomers of these compounds. Capillary electrophoresis, using cyclodextrins as chiral selectors, and HPLC, using a Pirkle type stationary phase, were tested. Baseline separation was not achieved by HPLC, but good separations were obtained in less than 7 min, by capillary electrophoresis with phosphate buffers pH 2.5-3 using sulfated-beta-cyclodextrin or mixtures of neutral beta-cyclodextrins as chiral selectors.
Simplício
Ana Luísa
AL
School of Pharmacy, Trinity College, Dublin 2, Ireland. anas@ibet.pt
Matias
Pedro
P
Gilmer
John F
JF
Clancy
John M
JM
D000588:Amines / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007189:Indans / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D007659:Ketones / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D004364:Pharmaceutical Preparations / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D011427:Propiophenones / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry
D000588:Amines; D007189:Indans; D007659:Ketones; D004364:Pharmaceutical Preparations; D011427:Propiophenones; D047392:beta-Cyclodextrins