Nuclear matrices and matrix attachment regions from Green alga: Dunaliella salina.
Nuclear DNA of eukaryotic organism attaches to the proteinaceous nuclear matrices via specific matrix attachment regions (MARs). In order to investigate the interactions between chromosomal DNA and nuclear matrices,we isolated the MARs from unicellular alga Dunaliella salina. As the first step,a random MAR library was set up and then the binding affinity of the selected clones to nuclear matrices was tested in this study. Three DNA fragments were found to bind specifically to the nuclear matrices in vitro,of which two were strong binders and all contained known consensus motifs and a hairpin loop structure of MAR.
Wang
Tian-Yun
TY
Laboratory for Cell Biology, Zhengzhou University, Zhengzhou 450052, China.
Hou
Wei-Hong
WH
Chai
Yu-Rong
YR
Ji
Xiang
X
Wang
Jian-Min
JM
Xue
Le-Xun
LX
D001483:Base Sequence; D000460:Chlorophyta / Q000235:genetics / Q000378:metabolism*; D053938:DNA Fragmentation; D018744:DNA, Plant / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D045170:Matrix Attachment Regions / Q000502:physiology*; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000378:metabolism*; D011487:Protein Conformation
D018744:DNA, Plant; D004268:DNA-Binding Proteins