Endothelin-3 production by human rhabdomyosarcoma: a possible new marker with a paracrine role.
Several autocrine and paracrine growth factor circuits have been found in human rhabdomyosarcoma cells. In this study we show that endothelin-3 (ET-3), a vasoactive peptide, is produced by human rhabdomyosarcoma cell lines, whereas it is not expressed by human sarcoma cell lines of non-muscle origin. We did not find evidence of a significant autocrine loop; nevertheless ET-3 produced by rhabdomyosarcoma cells can act as a paracrine factor, since it promotes migration of endothelial cells. Moreover ET-3 is present in plasma of mice bearing xenografts of human rhabdomyosarcoma cells, and may be potential new marker of the human rhabdomyosarcoma to be studied further.
Palladini
Arianna
A
Cancer Research Section, Department of Experimental Pathology, University of Bologna, Viale Filopanti 22, I-40126 Bologna, Italy.
Astolfi
Annalisa
A
Croci
Stefania
S
De Giovanni
Carla
C
Nicoletti
Giordano
G
Rosolen
Angelo
A
Sartori
Francesca
F
Lollini
Pier-Luigi
PL
Landuzzi
Lorena
L
Nanni
Patrizia
P
D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D019334:Endothelin-3 / Q000096:biosynthesis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D009842:Oligopeptides / Q000494:pharmacology; D012516:Osteosarcoma / Q000378:metabolism; D019899:Paracrine Communication; D010880:Piperidines / Q000494:pharmacology; D017466:Receptors, Endothelin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012208:Rhabdomyosarcoma / Q000378:metabolism*; D012512:Sarcoma, Ewing / Q000378:metabolism
D014408:Biomarkers, Tumor; D019334:Endothelin-3; D009842:Oligopeptides; D010880:Piperidines; D017466:Receptors, Endothelin; C086539:BQ 788