Increased interleukin-6 messenger RNA expression in macrophage-cocultured endometrial stromal cells in adenomyosis.
OBJECTIVE
To determine the effects of macrophage on endometrial stromal cells (ESCs) in women with adenomyosis.


METHODS
Eutopic endometrium was obtained and separated into single ESC in 10 women with adenomyosis (study group) and 11 without adenomyosis (control group). ESCs were then cultured alone or with macrophage for 24 hr.


RESULTS
Immunohistochemistry identified the presence of interleukin-6 (IL-6), IL-8, and IL-10 in ESCs. Real-time quantitative PCR revealed that the IL-6 mRNA was significantly expressed in macrophage-cocultured ESCs in adenomyosis than that in the controls, but was not different in ESCs cultured alone between the two groups. The levels of IL-8 and IL-10 mRNA were similar in ESCs either cultured alone or with macrophage between women with and without adenomyosis.


CONCLUSIONS
IL-6 mRNA was significantly expressed in ESCs after in vitro coculture with macrophage in adenomyosis. This aberrant behavior of ESCs might play a role in the formation of ectopic endometrial implants in adenomyosis.
Yang
Jehn-Hsiahn
JH
Department of Obstetrics and Gynecology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipie, Taiwan.
Wu
Ming-Yih
MY
Chang
Daw-Yuan
DY
Chang
Chien-Huei
CH
Yang
Yu-Shih
YS
Ho
Hong-Nerng
HN
D000328:Adult; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D004715:Endometriosis / Q000276:immunology* / Q000473:pathology; D004717:Endometrium / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000235:genetics*; D008264:Macrophages / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000276:immunology* / Q000473:pathology
D015850:Interleukin-6; D012333:RNA, Messenger