Polymer-templated microarrays for highly reliable plaque purification.
To infect cells with a particular multiplicity of infection, it is essential to know the concentration of virus in the inoculum. Here we describe a highly reliable and controllable method for plaque purification using cell-repellent surfaces micropatterned on the substrate. Micropatterning of localized chemical or biochemical domains has the potential to become a powerful tool in controlling the seeding of cells. The cell array was reliably fabricated with micropatterned surfaces, and the number of cells in a pattern was easily controlled by the cell density in the media and micropattern size. The cell micropatterns were infected with baculoviruses to form an array of virus plaques. GFP-modified and wild-type baculoviruses were used to verify the feasibility of purifying a specific plaque. Using confocal microscopy, GFP-expressing plaques were readily selectable and removable.
Na
Kyounga
K
Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, Korea.
Lee
Myungsoon
M
Shin
Byungcheol
B
Je
Yeonho
Y
Hyun
Jinho
J
D000818:Animals; D002452:Cell Count / Q000379:methods*; D002460:Cell Line; D002477:Cells / Q000821:virology; D002478:Cells, Cultured; D046228:Microarray Analysis; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D010948:Viral Plaque Assay / Q000379:methods*; D014780:Viruses / Q000302:isolation & purification*
D011108:Polymers