title: Development of a competitor DNA template of the 38 kDa gene for molecular quantification of M. tuberculosis.
abstract: The molecular quantification of Mycobacterium tuberculosis (TB) from clinical samples can improve the management of TB. Competitive polymerase chain reaction (C-PCR) is an accepted technique often used for this purpose, and IS6110 is the most popular target in such studies. As the number of these elements varies from 0 to 16 in clinical isolates, it is prone to give inconsistent results. A simple PCR-based approach is described in this study to generate a novel competitor for a single copy 38 kDa gene for the development of C-PCR for the quantification of the M. tuberculosis genome.
authors.affiliation: Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, India.
authors.forename: A
authors.initials: A
authors.lastname: Dwivedi
authors.forename: P K
authors.initials: PK
authors.lastname: Sehajpal
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000235:genetics*; D006801:Humans; D008074:Lipoproteins / Q000235:genetics*; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
chemical_list: C075966:Ag38 antigen, Mycobacterium tuberculosis; D000942:Antigens, Bacterial; D008074:Lipoproteins