Effect of Danchunwhangagam on LPS or DFX-induced cytokine production in peripheral mononuclear cells of cerebral infarction patients.
The Danchunwhangagam (DCWGG) has long been used for various cerebrovascular diseases. However, little scientific investigation has been carried out. Cytokines involved in the regulation of inflammatory reactions and immune responses may play a role in the pathogenesis of cerebral infarction (CI). The aim of the present study is to investigate the effect of DCWGG on the production of proinflammatory cytokines in peripheral blood mononuclear cells (PBMCs) from CI patients. The amount of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1alpha, IL-1beta, IL-6, and IL-8 in PBMC culture supernatant was significantly increased in the lipopolysaccaride (LPS)- or desferrioxamine (DFX)-treated cells compared with unstimulated cells. We showed that DCWGG inhibited the production of TNF-alpha, IL-1alpha, IL-1beta IL-6, and IL-8 induced by LPS in a dose-dependent manner. Also, DCWGG inhibited TNF-alpha, IL-1alpha, IL-beta, IL-6, and IL-8 production-induced DFX in dose-dependent manner. These results suggest that DCWGG might have regulatory effects on LPS- or DFX-induced cytokine production, which might explain its beneficial effect in the treatment of CI.
Kim
Su-Jin
SJ
College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Jeong
Hyun-Ja
HJ
Moon
Phil-Dong
PD
Lee
Kang-Min
KM
Moon
Byung-Soon
BS
Myung
Noh-Yil
NY
An
Nyeon-Hyung
NH
Hong
Seung-Heon
SH
Um
Jae-Young
JY
Kim
Hyung-Min
HM
D000368:Aged; D002478:Cells, Cultured; D002544:Cerebral Infarction / Q000097:blood* / Q000188:drug therapy; D016207:Cytokines / Q000378:metabolism*; D003676:Deferoxamine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007378:Interleukins / Q000378:metabolism; D007723:Korea; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008518:Medicine, East Asian Traditional; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007378:Interleukins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D003676:Deferoxamine