Growth of canola (Brassica napus) in the presence of plant growth-promoting bacteria and either copper or polycyclic aromatic hydrocarbons.
Growth of canola (Brassica napus) seeds treated with plant growth-promoting bacteria in copper-contaminated and polycyclic aromatic hydrocarbon (PAH)-contaminated soils was monitored. Pseudomonas asplenii AC, isolated from PAH-contaminated soil, was transformed to express a bacterial gene encoding 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and both native and transformed bacteria were tested for growth promotion. Inoculation of seeds, grown in the presence of copper or creosote, with either native or transformed P. asplenii AC significantly increased root and shoot biomass. Native and transformed P. asplenii AC and transformed P. asplenii AC encapsulated in alginate were equally effective at promoting plant growth in copper-contaminated soils. In creosote-contaminated soils the native bacterium was the least effective, and the transformed encapsulated bacterium was the most effective in growth promotion.
Reed
M L E
ML
Department of Biology, University of Waterloo, ON, Canada.
Glick
Bernard R
BR
D000383:Agriculture / Q000379:methods; D000464:Alginates; D029688:Brassica napus / Q000254:growth & development* / Q000382:microbiology; D003300:Copper; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D011084:Polycyclic Aromatic Hydrocarbons; D011549:Pseudomonas / Q000502:physiology*; D012639:Seeds / Q000254:growth & development / Q000382:microbiology; D012988:Soil Microbiology; D012989:Soil Pollutants
D000464:Alginates; D006603:Hexuronic Acids; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D003300:Copper; D020723:Glucuronic Acid