title: Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines.
abstract: Mantle-cell lymphoma (MCL) is genetically characterized by 11q13 chromosomal translocations involving the CCND1 gene. We have characterized five MCL cell lines, JVM-2, GRANTA-519, REC-1, JEKO-1, and NCEB-1, combining metaphase and array comparative genomic hybridization, multicolor-FISH, and molecular analysis. Our results revealed common gained regions at 2p14, 9q31.2-qter, 11q13.1-q21, 13q14-q21.2, 13q34-qter and 18q21.1-q22.1, and losses at 1p21.2-p31.1, 2p11.2, 8p21.2-pter, 9p21.3-pter, 11q23.3-qter, 17p11.2-pter, and 17q21.2-q22.2. All cell lines except JVM-2, displayed moderate or high numerical chromosome instability. In addition, an ongoing level of chromosome rearrangements was observed in REC-1. Surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. Our findings indicate that these cell lines represent three patterns of chromosome evolution in MCL and may be useful to understand the pathogenesis of this neoplasm.
authors.affiliation: Departament de Biologia Cellular, Fisiologia i Immunologia and Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Camps
authors.forename: Itziar
authors.initials: I
authors.lastname: Salaverria
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Garcia
authors.forename: Esther
authors.initials: E
authors.lastname: Prat
authors.forename: Sílvia
authors.initials: S
authors.lastname: Beà
authors.forename: Jessica C
authors.initials: JC
authors.lastname: Pole
authors.forename: Lluis
authors.initials: L
authors.lastname: Hernández
authors.forename: Javier
authors.initials: J
authors.lastname: Del Rey
authors.forename: Juan Cruz
authors.initials: JC
authors.lastname: Cigudosa
authors.forename: Marta
authors.initials: M
authors.lastname: Bernués
authors.forename: Carlos
authors.initials: C
authors.lastname: Caldas
authors.forename: Dolors
authors.initials: D
authors.lastname: Colomer
authors.forename: Rosa
authors.initials: R
authors.lastname: Miró
authors.forename: Elías
authors.initials: E
authors.lastname: Campo
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis / Q000379:methods; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping; D020522:Lymphoma, Mantle-Cell / Q000235:genetics*; D008677:Metaphase; D051379:Mice; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods