Apoptosis mechanisms of human gastric cancer cell line MKN-45 infected with human mutant p27.
OBJECTIVE
To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms.


METHODS
The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells.


RESULTS
Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4+/-3.12%) compared to the control group (3.12+/-0.13%, P < 0.01).


CONCLUSIONS
Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro.
Zhu
Jin-Shui
JS
Department of Gastroenterology, Affiliated Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China. zhujs1803@hotmail.com
Wang
Long
L
Cheng
Guo-Qiang
GQ
Li
Qin
Q
Zhu
Zu-Ming
ZM
Zhu
Li
L
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016296:Mutagenesis; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000473:pathology* / Q000503:physiopathology*
D050760:Cyclin-Dependent Kinase Inhibitor p27