Immunohistochemistry as a method to study elastic fibers of human vocal fold.
OBJECTIVE
Verify the use of immunohistochemistry as a method to measure all forms of elastic fibers at human vocal folds.


METHODS
Transversal cohort.


METHODS
We collected vocal folds following these criterion: age between 25 and 40, Caucasian men, dead by gun shot, within 12 hours of death, without instrumentation of the larynx or suspicion of neck injury and without mucosal lesions noted by microscopy. Ten vocal folds were collected and one, of a man aged 28 years, was selected to study. The vocal fold was transversely cut in 9 regions and in each segment three slides were made. These slides were stained by Verhoeff and Weighert's resorcin-fuchsin and used for immunohistochemistry. The elastic compound was measured by colorimetric software analysis.


RESULTS
In Verhoeff and Weighert's resorcin-fuchsin, the intermediate and deep layer showed values higher than those of the superficial layer. The amount of tropoelastin identified by the antibody at the superficial layer was close to those of intermediate and deep layer.


CONCLUSIONS
Immunohistochemistry is a method that can identify and measure all forms of elastic fibers at human vocal fold.
Ramos
Hugo Valter Lisboa
HV
UNIFESP, Escola Paulista de Medicina.
Simões
Manuel de Jesus
Mde J
Pontes
Paulo Augusto de Lima
PA
Neves
Luciano Rodrigues
LR
Barbosa
Luiz Henrique Fonceca
LH
De Biase
Noemi Grigoletto
NG
Oshima
Celina T S
CT
D000328:Adult; D004396:Coloring Agents; D003430:Cross-Sectional Studies; D004547:Elastic Tissue / Q000033:anatomy & histology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007830:Larynx / Q000033:anatomy & histology; D008297:Male; D009412:Nerve Fibers; D013194:Staining and Labeling / Q000379:methods*; D014827:Vocal Cords / Q000033:anatomy & histology*
D004396:Coloring Agents