The maternal combined supplementation of folic acid and Vitamin B(12) suppresses ethanol-induced developmental toxicity in mouse fetuses.
Maternal ethanol consumption during pregnancy can induce developmental defects in the fetus. The objective of this study was to assess whether combined supplementation of folic acid (FA) and Vitamin B(12) (VB(12)) in dams would suppress ethanol-induced developmental toxicity in CD-1 mice. Ethanol (5.0 g/kg) was given intragastrically from gestational day (GD) 6 to GD15. Vitamin supplementation groups were additionally given 60.0 mg/kg FA, 1.0 mg/kg VB(12), or 60.0 mg/kg FA+1.0 mg/kg VB(12) during GD1-16. The control group received distilled water only. Results of litter evaluation on GD18 showed that combined supplementation of FA and VB(12) ameliorated many of the adverse effects of ethanol. In contrast, the single vitamin supplementation groups showed little or no amelioration. These results suggest that combined supplementation of FA and VB(12) was more effective than each vitamin toward suppressing ethanol-induced developmental toxicity in CD-1 mice.
Xu
Yajun
Y
Department of Nutrition and Food Hygiene, Laboratory of Molecular Toxicology and Developmental Molecular Biology, School of Public Health, Peking University, Beijing 100083, China.
Li
Yong
Y
Tang
Yunan
Y
Wang
Junbo
J
Shen
Xiaoyi
X
Long
Zhu
Z
Zheng
Xiaoying
X
D000014:Abnormalities, Drug-Induced / Q000209:etiology / Q000517:prevention & control; D000435:Alcoholic Intoxication / Q000209:etiology / Q000517:prevention & control; D000818:Animals; D001842:Bone and Bones / Q000002:abnormalities / Q000187:drug effects / Q000196:embryology; D019587:Dietary Supplements; D010064:Embryo Implantation / Q000187:drug effects; D020964:Embryo Loss / Q000139:chemically induced / Q000517:prevention & control; D000431:Ethanol / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000633:toxicity*; D005260:Female; D047109:Fetal Development / Q000187:drug effects*; D005317:Fetal Growth Retardation / Q000139:chemically induced / Q000517:prevention & control; D005492:Folic Acid / Q000008:administration & dosage* / Q000097:blood / Q000627:therapeutic use; D005865:Gestational Age; D006710:Homocysteine / Q000097:blood / Q000187:drug effects; D051379:Mice; D011247:Pregnancy; D014805:Vitamin B 12 / Q000008:administration & dosage* / Q000097:blood / Q000627:therapeutic use
D006710:Homocysteine; D000431:Ethanol; D005492:Folic Acid; D014805:Vitamin B 12