title: Use of PCR-restriction enzyme pattern analysis and sequencing database for hsp65 gene-based identification of Nocardia species.
abstract: Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s. Here we reassessed the capacity of PCR-restriction enzyme pattern analysis (PRA) of the hsp65 gene to differentiate Nocardia species, including 36 new species. Our results confirm that hsp65 PRA must no longer be used for Nocardia species identification, as many species have the same restriction pattern. We then compared sequencing-based strategies using an hsp65 database and a 16S rRNA database and found that the hsp65 region contained sufficient polymorphisms for comprehensive Nocardia species identification.
authors.affiliation: UMR CNRS 5557, Laboratoire de Mycologie Fondamentale et Appliquée aux Biotechnologies Industrielles, UFR Pharmacie, UCB Lyon I, 69373, Lyon Cedex 08, France.
authors.forename: Verónica
authors.initials: V
authors.lastname: Rodríguez-Nava
authors.forename: Andrée
authors.initials: A
authors.lastname: Couble
authors.forename: Gregory
authors.initials: G
authors.lastname: Devulder
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Flandrois
authors.forename: Patrick
authors.initials: P
authors.lastname: Boiron
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laurent
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D030541:Databases, Genetic; D020459:Genes, rRNA; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D009615:Nocardia / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D012336:RNA, Ribosomal, 16S