Comparing reagents for efficient transfection of human primary myoblasts: FuGENE 6, Effectene and ExGen 500.
This study compared three different synthetic reagents (FuGENE 6, Effectene and ExGen 500) for the transfection of human primary myoblasts. We examined the efficiency, cytotoxicity and size of the complexes formed in the presence of different amounts of vector and DNA and with variable amounts of serum. Transfection rates were relatively high for primary cells, especially with FuGENE 6 (20%), which appeared to be the best transfection reagent for these cells, even in the presence of 10% serum. Cultured human myoblasts are an interesting tool for studying neuromuscular diseases and are potentially useful for myoblast transfer therapy studies. Moreover, the efficiency of these transfection reagents in a medium containing 10% serum is promising for possible gene therapy protocols for muscle diseases.
Arnold
Anne-Sophie
AS
Laboratoire Commun, LC 001 CNRS, Faculté de Pharmacie, Université Louis Pasteur Strasbourg-I, 74 route du Rhin, BP 60024, 67401 Illkirch Cedex, France.
Laporte
Vincent
V
Dumont
Serge
S
Appert-Collin
Aline
A
Erbacher
Patrick
P
Coupin
Gilliane
G
Levy
Rachel
R
Poindron
Philippe
P
Gies
Jean-Pierre
JP
D018929:Cell Culture Techniques; D002470:Cell Survival; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry* / Q000633:toxicity; D032448:Myoblasts, Skeletal / Q000187:drug effects / Q000378:metabolism*; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry* / Q000633:toxicity*; D014162:Transfection / Q000379:methods
C498175:Effectene; C411955:FuGene; D007202:Indicators and Reagents; D008055:Lipids; D011094:Polyethyleneimine; D004247:DNA