title: Therapeutic outcome of hyperbaric oxygen and basic fibroblast growth factor on intractable skin ulcer in legs: preliminary report.
abstract: BACKGROUND
  Beneficial effects of hyperbaric oxygen on ischemic vascular diseases have been noted. Acceleration of wound healing with basic fibroblast growth factor has also been reported. The authors employed combination therapy of hyperbaric oxygen and basic fibroblast growth factor in patients with skin ulcer in legs refractory to conventional therapy.
  
  
  METHODS
  Three men and four women were simultaneously treated with hyperbaric oxygen at 2 absolute atmospheric pressures for 90 minutes daily and spray treatment of basic fibroblast growth factor to the ulcer bed daily for an average of 2.6 months. Biopsy specimens obtained from ulcer tissues were divided into two pieces, one for histologic examination and the other for measuring fibrous protein.
  
  
  RESULTS
  Ulcers were completely cured in five of seven patients. Two patients showed shrinkage of ulcer size. This combined therapy induced proliferation of connective tissue of the ulcer tissues, especially collagen and noncollagenous protein.
  
  
  CONCLUSIONS
  Combined treatment with hyperbaric oxygen and basic fibroblast growth factor may be useful in patients with intractable skin ulcers in legs, and the shrinkage effect of this therapy is probably related to the proliferation of granulation tissues of the ulcer lesion.
authors.affiliation: Hyperbaric Oxygenation Institute, Division of Urology, Saito Rosai Hospital, Chiba, Japan. nakada@kdp.biglobe.ne.jp
authors.forename: Teruhiro
authors.initials: T
authors.lastname: Nakada
authors.forename: Yoriyuki
authors.initials: Y
authors.lastname: Saito
authors.forename: Masaru
authors.initials: M
authors.lastname: Chikenji
authors.forename: Shinichi
authors.initials: S
authors.lastname: Koda
authors.forename: Michio
authors.initials: M
authors.lastname: Higuchi
authors.forename: Kinya
authors.initials: K
authors.lastname: Kawata
authors.forename: Shu
authors.initials: S
authors.lastname: Ishida
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takahashi
authors.forename: Shigeo
authors.initials: S
authors.lastname: Kondo
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kubota
authors.forename: Isao
authors.initials: I
authors.lastname: Kubota
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Shimizu
mesh_terms: D000368:Aged; D048909:Diabetes Complications / Q000628:therapy; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D006801:Humans; D006931:Hyperbaric Oxygenation; D007871:Leg Ulcer / Q000473:pathology / Q000628:therapy*; D008297:Male; D008875:Middle Aged
chemical_list: D016222:Fibroblast Growth Factor 2