High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.
Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
Aires-de-Sousa
M
M
Department of Periodontology, University Hospital Münster, Waldeyerstrasse 30, D-48149 Münster, Germany.
Boye
K
K
de Lencastre
H
H
Deplano
A
A
Enright
M C
MC
Etienne
J
J
Friedrich
A
A
Harmsen
D
D
Holmes
A
A
Huijsdens
X W
XW
Kearns
A M
AM
Mellmann
A
A
Meugnier
H
H
Rasheed
J K
JK
Spalburg
E
E
Strommenger
B
B
Struelens
M J
MJ
Tenover
F C
FC
Thomas
J
J
Vogel
U
U
Westh
H
H
Xu
J
J
Witte
W
W
D015373:Bacterial Typing Techniques / Q000379:methods / Q000706:statistics & numerical data; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007753:Laboratories; D015588:Observer Variation; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods / Q000706:statistics & numerical data; D012984:Software; D013203:Staphylococcal Infections / Q000382:microbiology; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial; D013205:Staphylococcal Protein A