An amplified mass piezoelectric immunosensor for Schistosoma japonicum.
An ultrasensitive piezoelectric immunosensor using an amplification path based on an insoluble biocatalyzed precipitation product has proposed for Schistosoma japonicum. A mercapto Schistosoma japonicum antigen was self-assembled onto the quartz crystal surface via an Au nanoparticle mediator monolayer to sense the Schistosoma japonicum antibody (SjAb). And the horseradish peroxidase labeled protein A conjugate which was bounded to the SjAb by a "sandwich" format was used as a biocatalyst for the oxidative precipitation of 4-chloro-1-naphthol by H(2)O(2) to yield the insoluble product benzo-4-chlorohexadienone, resulting in an amplified mass sensing of antigen-antibody interaction. The amount of the precipitate accumulated on the quartz crystal is controlled by the antibody concentration. The SjAb can be linearly determined in the range of 10-200 ngml(-1) and the detection limit reaches as low as 5 ngml(-1).
Wu
Zhaoyang
Z
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. zuwu@hnu.cn
Wu
Jian
J
Wang
Shiping
S
Shen
Guoli
G
Yu
Ruqin
R
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth; D015374:Biosensing Techniques / Q000295:instrumentation*; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D012549:Schistosoma japonicum / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007106:Immune Sera