Synthesis and characterization of new biodegradable hyaluronan alkyl derivatives.
Several new biocompatible and degradable materials were prepared by chemical modification of sodium hyaluronate. The method of activation of hyaluronate by cyanogen bromide was used and subsequent reaction with nucleophile led to the formation of carbamate. This modification of hydroxyl groups of glycosaminoglycans preserves the carboxyl groups and retains properties of polyelectrolyte. This method affords derivatives easily and the reaction condition correlates with degree of substitution. The experimental results show the effect of reaction conditions (reaction time, ratio of reactants) and effect of substitution on biodegradability. The obtained materials were characterized by nuclear magnetic resonance and Fourier transform infrared spectroscopy.
Mlcochová
Petra
P
CPN spol. s r.o., Dolní Dobrouc, Czech Republic. mlcochova@contipro.cz
Bystrický
Slavomír
S
Steiner
Bohumil
B
Machová
Eva
E
Koós
Miroslav
M
Velebný
Vladimír
V
Krcmár
Martin
M
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis; D001673:Biodegradation, Environmental; D002219:Carbamates / Q000138:chemical synthesis; D002417:Cattle; D002850:Chromatography, Gel; D003488:Cyanogen Bromide / Q000737:chemistry; D006025:Glycosaminoglycans / Q000737:chemistry; D006820:Hyaluronic Acid / Q000138:chemical synthesis* / Q000737:chemistry*; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D007700:Kinetics; D008297:Male; D019906:Nuclear Magnetic Resonance, Biomolecular; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D002219:Carbamates; D006025:Glycosaminoglycans; D014867:Water; D017665:Hydroxyl Radical; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase; D003488:Cyanogen Bromide