Cell surface expression of a chimeric protein containing mouse immunoglobulin G1 Fc domain and its immunological property.
Recently we reported that a chimeric molecule containing mouse transferrin receptor and immunoglobulin G1 (IgG1) Fc, mTR-Fc, induced higher immune responses and can be used as a vaccine adjuvant. In this study, the immunological property of the molecule was investigated. Although, the mTR-Fc did not activate complement classical pathway, it was recognized by activated macrophage as like intact IgG Fc, which is recognized by macrophage via Fcgamma receptor. In addition, we found that splenocyte simultaneously exposed to lipopolysaccaride (LPS) and mTR-Fc produced higher amount of interleukin-10, comparing to that exposed to only LPS. These results suggest that the mTR-Fc molecules conserved the IgG Fc property to biasing immune responses via modulation of cytokine production by antigen presenting cell.
Batanova
Tatiana A
TA
Department of Veterinary Parasitological Diseases, Gifu University, Gifu 501-1193, Japan.
Ota
Haruko
H
Kitoh
Katsuya
K
Matsumoto
Yasunobu
Y
Hayashi
Yoshihiro
Y
Takashima
Yasuhiro
Y
D000818:Animals; D007074:Immunoglobulin G / Q000276:immunology* / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D011990:Receptors, Transferrin / Q000276:immunology* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000276:immunology
D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D011990:Receptors, Transferrin; D011993:Recombinant Fusion Proteins; D016753:Interleukin-10