[Selection of the peptides specifically binding to hepatoma by using phage display in vivo].
OBJECTIVE
To screen the peptides binding to hepatoma specifically.


METHODS
Nude mice were inoculated with human tumor cells BEL-7402, then the Ph.D.-12 Phage Display Peptide Library was injected intravenously (tail vein) into mice. After 20 min the mice were sacrificed and the phage rescued from tumor tissues. All the tissues should be made an appraisal using immunohistochemistry and titering. The phage recovered from the tissues were amplified and purified then re-injected for next round screening. After screening for 3 rounds in vivo, the phage-peptides that homed to the tumor tissues or cells were obtained. Then these phage clones were sequenced to analyze the motif. All the sequenced clones were appraised by using cell ELISA and titering the distribution in vivo.


RESULTS
Through the appraisal from vivo and vitro and peptides' sequences, several target motifs were preliminarily determined.


CONCLUSIONS
Some phage-peptides which could specifically bind tumor cells or tissues can be selected successfully from the random twelve-peptide library by means of phage display in vivo.
Du
Bing
B
School of Life Science, East China Normal University, Shanghai 200062, China.
Yu
Jing
J
Zhou
Zhong-liang
ZL
Zhang
Ping
P
Yu
Meng
M
Qian
Min
M
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding
D019151:Peptide Library; D010455:Peptides