title: The structural proteome of Pseudomonas aeruginosa bacteriophage phiKMV.
abstract: The structural proteome of phiKMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in phiKMV and suggest subtle structural differences from phage T7.
authors.affiliation: Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
authors.affiliation: Biomedical Research Institute, Limburgs Universitair Centrum and School of Life Sciences, University Hasselt, Diepenbeek, B-3590, Belgium.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Noben
authors.affiliation: Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Hertveldt
authors.affiliation: Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
authors.forename: Pieter-Jan
authors.initials: PJ
authors.lastname: Ceyssens
authors.affiliation: Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
authors.forename: Yves
authors.initials: Y
authors.lastname: Briers
authors.affiliation: Biomedical Research Institute, Limburgs Universitair Centrum and School of Life Sciences, University Hasselt, Diepenbeek, B-3590, Belgium.
authors.forename: Debora
authors.initials: D
authors.lastname: Dumont
authors.affiliation: Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Roucourt
authors.affiliation: State Institute for Genetics and Selection of Industrial Micro-organisms, 1st Dorozhnii proezd 1, Moscow, 113545 Russia.
authors.forename: Victor N
authors.initials: VN
authors.lastname: Krylov
authors.affiliation: Howard Hughes Medical Institute; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Street 16/10, Moscow, 117991 Russia.|Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
authors.forename: Vadim V
authors.initials: VV
authors.lastname: Mesyanzhinov
authors.affiliation: Biomedical Research Institute, Limburgs Universitair Centrum and School of Life Sciences, University Hasselt, Diepenbeek, B-3590, Belgium.
authors.forename: Johan
authors.initials: J
authors.lastname: Robben
authors.affiliation: Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
authors.forename: Guido
authors.initials: G
authors.lastname: Volckaert
mesh_terms: D017123:Bacteriophage T7 / Q000235:genetics; D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D016679:Genome, Viral; D013058:Mass Spectrometry; D020543:Proteome; D011550:Pseudomonas aeruginosa / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020543:Proteome; D014764:Viral Proteins