Stemphones, novel potentiators of imipenem activity against methicillin-resistant staphylococcus aureus, produced by Aspergillus sp. FKI-2136.
A fungal strain FKI-2136 identified as genus Aspergillus was found to produce potentiators of imipenem activity against methicillin-resistant Staphylococcus aureus (MRSA). Two new compounds designated stemphones B and C were isolated along with a structurally related known compound cochlioquinone D from the fermentation broth of the producing strain by solvent extraction, silica gel column chromatography and preparative HPLC. These compounds have a common tetracyclic quinone skeleton. Stemphone C potentiated imipenem activity against the MRSA 512 fold by decreasing MIC value of imipenem from 16 microg/ml to 0.03 microg/ml.
Koyama
Nobuhiro
N
School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Nagahiro
Tomoko
T
Yamaguchi
Yuichi
Y
Masuma
Rokuro
R
Tomoda
Hiroshi
H
Omura
Satoshi
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000145:classification / Q000378:metabolism*; D016227:Benzoquinones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D005591:Chemical Fractionation; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004357:Drug Synergism; D005285:Fermentation; D015378:Imipenem / Q000494:pharmacology*; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D013211:Staphylococcus aureus / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D016227:Benzoquinones; C087469:stemphone; D015378:Imipenem