Interactions of high amylose starch and deoxycholic acid on gut functions in rats.
OBJECTIVE
This study evaluated whether high amylose cornstarch (HAS) could prevent adverse physiologic effects induced by deoxycholic acid (DCA) in the gut of rats.


METHODS
Thirty-two male Sprague-Dawley rats were provided with a low amylose cornstarch (LAS) diet or a 50/50 mixture of LAS and HAS diets for 4 wk; each of these diets was supplemented with 0 or 2 g of DCA per kilogram of diet. Therefore, there were four groups.


RESULTS
Cecal content pH was lower in rats fed the HAS diets compared with rats fed the LAS diets (P < 0.05). Bifidobacteria number in cecal contents, cecal pools of acetate, butyrate, and total short-chain fatty acids were highest in rats fed the HAS diet; moreover, the HAS/DCA diet resulted in increased Bifidobacteria growth and short-chain fatty acid production numerically compared with the LAS/DCA diets (P = 0.06). Production of prostaglandin-E2 in colonic mucosa was highest in rats fed the LAS/DCA diet, and the intake of HAS significantly decreased prostaglandin-E2 levels (P < 0.05).


CONCLUSIONS
In this study, DCA may have inhibited fermentation in the large intestine and increased prostaglandin-E2 production, and concurrent administration of HAS and DCA may have decreased the adverse effects on the gut induced by DCA.
Chang
Moon Jeong
MJ
Department of Food and Life Science, Kookmin University, Seoul, Korea.
Soel
So Mi
SM
Bang
Myung Hee
MH
Park
Jung Han Yoon
JH
Kang
Nam E
NE
Kim
Woo Kyoung
WK
D000688:Amylose / Q000008:administration & dosage / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D001644:Bifidobacterium / Q000254:growth & development* / Q000302:isolation & purification; D002432:Cecum / Q000737:chemistry / Q000382:microbiology; D015169:Colony Count, Microbial; D003840:Deoxycholic Acid / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005232:Fatty Acids, Volatile / Q000032:analysis*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013213:Starch / Q000008:administration & dosage / Q000494:pharmacology
D005232:Fatty Acids, Volatile; D003840:Deoxycholic Acid; D013213:Starch; D000688:Amylose; D015232:Dinoprostone