Expression and activity patterns of nitric oxide synthases and antioxidant enzymes reveal a substantial heterogeneity between cardiac and vascular aging in the rat.
We investigated the effects of aging and ischemia-reperfusion (I/R) injury on the expression and activity of nitric oxide (*NO) synthases and superoxide dismutase (SOD) isoforms. To this end we perfused excised hearts from young (6 months old) and old (31-34 months old) rats according to the Langendorff technique. The isolated hearts were, after baseline perfusion for 30 min, either subjected to 20 min of global no-flow ischemia followed by 40 min of reperfusion or were control-perfused (60 min normoxic perfusion). Both MnSOD and Cu,ZnSOD expression remained unchanged with increasing age and remained unaltered by I/R. However, SOD activity decreased from 7.55 +/- 0.1 U/mg protein in young hearts to 5.94 +/- 0.44 in old hearts (P<0.05). Furthermore, I/R led to a further decrease in enzyme activity (to 6.35 +/- 0.41 U/mg protein; P<0.05) in myocardium of young, but not in that of old animals. No changes in myocardial protein-bound 3-nitrotyrosine levels could be detected. Endothelial NOS (eNOS) expression and activity remained unchanged in aged left ventricles, irrespective of I/R injury. This was in steep contrast to peripheral (renal and femoral) arteries obtained from the same animals where a marked age-associated increase of eNOS protein expression could be demonstrated. Inducible NOS expression was undetectable either in the peripheral arteries or in the left ventricle, irrespective of age. In particular when associated with an acute pathology, which is furthermore limited to a certain time frame, changes in the aged myocardium with respect to enzymes crucially involved in maintaining the redox homeostasis, seem to be much less pronounced or even absent compared to the vascular aging process. This may point to heterogeneity in the molecular regulation of the cardiovascular aging process.
van der Loo
Bernd
B
Division of Cardiology, Cardiovascular Centre, University Hospital Zurich, Rämistrasse 100, CH-8091, Zürich, Switzerland.
Bachschmid
Markus
M
Labugger
Ralf
R
Schildknecht
Stefan
S
Kilo
Juliane
J
Hahn
Raphael
R
Palacios-Callender
Miriam
M
Lüscher
Thomas F
TF
D000375:Aging / Q000378:metabolism*; D000818:Animals; D005263:Femoral Artery / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006321:Heart; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D017682:Myocardial Stunning; D009206:Myocardium / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000032:analysis / Q000378:metabolism; D010477:Perfusion / Q000379:methods; D051381:Rats; D011916:Rats, Inbred F344; D012077:Renal Artery / Q000378:metabolism; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D007527:Isoenzymes; C002744:3-nitrotyrosine; D014443:Tyrosine; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D013482:Superoxide Dismutase; D005978:Glutathione