Structural analysis of anti-tumor heteropolysaccharide GFPS1b from the cultured mycelia of Grifola frondosa GF9801.
A 21-kDa heteropolysaccharide, coded as GFPS1b, was obtained from the cultured mycelia of Grifola frondosa GF9801 by hot-water extraction, ethanol precipitation, and fractioned by DEAE Sepharose Fast-flow, followed by the purification with Sephadex G-100 column chromatography using an AKTA purifier. It exhibited more potent anti-proliferative activity on MCF-7 cells than other polysaccharide fractions. GFPS1b was an acidic polysaccharide with approximately 16.60% protein and 4.3% uronic acid. Gas chromatography of absolute acid hydrolysate of GFPS1b suggested that it was composed of D-glucose, D-galactose, and L-arabinose with a molar ratio of 4:2:1. Periodate oxidation, Smith degradation, partial acid hydrolyzation, methylation analysis, FT-IR, and (1)H, (13)C NMR spectroscopy analysis revealed that GFPS1b had a backbone consisting of alpha-(1-->4)-linked D-galacopyranosyl and alpha-(1-->3)-linked D-glucopyranosyl residues substituted at O-6 with glycosyl residues composed of alpha-L-arabinose-(1-->4)-alpha-D-glucose (1--> linked residues.
Cui
F J
FJ
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036, PR China. fengjiecui@sytu.edu.cn
Tao
W Y
WY
Xu
Z H
ZH
Guo
W J
WJ
Xu
H Y
HY
Ao
Z H
ZH
Jin
J
J
Wei
Y Q
YQ
D000970:Antineoplastic Agents / Q000737:chemistry*; D045744:Cell Line, Tumor; D048691:Grifola / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D025282:Mycelium / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism
D000970:Antineoplastic Agents; C517393:GFPS1b polysaccharide, Grifola frondosa; D011134:Polysaccharides