[Effect of testosterone propionate on the distribution pattern of calcitonin gene-related peptide in different motoneuron pools].
OBJECTIVE
To study the effect of testosterone propionate (TP) on the distribution pattern of calcitonin gene-related peptide (CGRP) in two types of motoneuron (Mn) pools in rats.


METHODS
The double labeling of cholera toxin B subunit coupled with colloidal gold (CB-Au) retrograde identification combining with immunocytochemistry was mainly used to reveal the distribution pattern of CGRP-like immunoreactivity (CGRP-LI) and its changes in the motoneuron pools labeled by CB-Au.


RESULTS
TP injected intramuscularly 28 days later significantly decreased CGRP expression in Mn pool innervating extensor digitorum longus (EDL, fast-twitch), comparing with corresponding control and castration group respectively (P < 0.001), while no significant effect on Mn pools innervating soleus (SOL, slow-twitch, P > 0.05) was observed.


CONCLUSIONS
EDL-Mn pool is more sensitive to testosterone propionate than SOL-Mn pool in regulating CGRP expression.
Shi
Ge-ming
GM
Department of Neurobiology, Institute of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050017, China.
Li
Shuang-cheng
SC
Zhang
Kai
K
Xing
Ling-xiao
LX
Cui
Hui-xian
HX
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009046:Motor Neurons / Q000187:drug effects* / Q000378:metabolism; D018656:Muscle Fibers, Fast-Twitch / Q000166:cytology* / Q000187:drug effects; D018657:Muscle Fibers, Slow-Twitch / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D043343:Testosterone Propionate / Q000494:pharmacology*
D015740:Calcitonin Gene-Related Peptide; D043343:Testosterone Propionate