Propolis protects human spermatozoa from DNA damage caused by benzo[a]pyrene and exogenous reactive oxygen species.
Many environmental, physiological and genetic factors have been implicated in defective sperm function, the most common cause of infertility. In addition, sperm preparation techniques such as centrifugation, used prior to in vitro fertilization, are associated with the generation of reactive oxygen species (ROS) and an increase in the level of DNA damage. Factors that can offer spermatozoa protection are, therefore, of great importance. This study was designed to examine in vitro the effect of a Chilean propolis ethanolic extract on human spermatozoa treated with benzo[a]pyrene and exogenous reactive oxygen species. Our experimental evidence demonstrated that the natural drug under investigation is able to protect genomic DNA by damage induced by benzo[a]pyrene, hydrogen peroxide (H2O2) and hydrogen peroxide in combination with adenosine 5'-diphosphate (ADP) and ferrous sulfate (FeSO4), determining a significant reduction of the intracellular oxidants. An increase in membrane damage, measured by monitoring the formation of thiobarbituric acid-reactive substances (TBARS) and lactic dehydrogenase (LDH) release, was observed only in sperm treated with H2O2, ADP and FeSO4. The propolis extract was shown to possess the capacity to protect sperm membrane from the deleterious action of oxidative attack, reducing TBARS formation and LDH release. In summary, our results evidence that the protective effect exhibited by this natural compound in human spermatozoa is correlated, at least in part, to the antioxidant capacity of its active components, and suggest that propolis may have a role in protection against male infertility.
Russo
A
A
Department of Biological Chemistry, Medical Chemistry and Molecular Biology, University of Catania, v.le A. Doria 6, 95125 Catania, Italy. alrusso@unict.it
Troncoso
N
N
Sanchez
F
F
Garbarino
J A
JA
Vanella
A
A
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001564:Benzo(a)pyrene / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects*; D000431:Ethanol; D005296:Ferrous Compounds / Q000494:pharmacology; D006801:Humans; D007770:L-Lactate Dehydrogenase; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D011429:Propolis / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology / Q000502:physiology*
D005296:Ferrous Compounds; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D001564:Benzo(a)pyrene; C020748:ferrous sulfate; D000431:Ethanol; D008315:Malondialdehyde; D000244:Adenosine Diphosphate; D011429:Propolis; D007770:L-Lactate Dehydrogenase