title: APOBEC3F and APOBEC3G mRNA levels do not correlate with human immunodeficiency virus type 1 plasma viremia or CD4+ T-cell count.
abstract: APOBEC3F and APOBEC3G (hA3F and hA3G) are part of an innate mechanism of antiretroviral defense. The human immunodeficiency virus type 1 (HIV-1) accessory protein Vif targets both proteins for proteasomal degradation. Using mRNA from peripheral blood mononuclear cells of 92 HIV-infected subjects not taking antiretroviral therapy and 19 HIV-uninfected controls, we found that hA3F (P < 0.001) and hA3G (P = 0.016) mRNA levels were lower in HIV-infected subjects and were positively correlated with one another (P = 0.003). However, we found no correlation in the abundance of either hA3F or hA3G mRNA with either viral load or CD4 counts in HIV-infected subjects.
authors.affiliation: Washington University School of Medicine, Division of Gastroenterology, Box 8124, 660 S. Euclid Ave., St. Louis, MO 63110, USA.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Cho
authors.forename: Henning
authors.initials: H
authors.lastname: Drechsler
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Burke
authors.forename: Max Q
authors.initials: MQ
authors.lastname: Arens
authors.forename: William
authors.initials: W
authors.lastname: Powderly
authors.forename: Nicholas O
authors.initials: NO
authors.lastname: Davidson
mesh_terms: D000071480:APOBEC-3G Deaminase; D018791:CD4 Lymphocyte Count; D002478:Cells, Cultured; D003564:Cytidine Deaminase; D043525:Cytosine Deaminase / Q000235:genetics*; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D009700:Nucleoside Deaminases / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D012097:Repressor Proteins / Q000235:genetics*; D019562:Viral Load; D014766:Viremia
chemical_list: D012333:RNA, Messenger; D012097:Repressor Proteins; D009700:Nucleoside Deaminases; C492906:APOBEC3F protein, human; D043525:Cytosine Deaminase; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase