Dominance relationships between self-incompatibility alleles controlled by DNA methylation.
In crucifers, the pollen S-determinant gene, SP11, is sporophytically expressed in the anther tapetum, and the pollen self-incompatibility phenotype is determined by the dominance relationships between the two S-haplotypes it carries. We report here that 5' promoter sequences of recessive SP11 alleles are specifically methylated in the tapetum before the initiation of SP11 transcription. These results suggest that tissue-specific monoallelic de novo DNA methylation is involved in determining the dominance interactions that determine the cruciferous self-incompatibility phenotype.
Shiba
Hiroshi
H
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan. hshiba@salk.edu
Kakizaki
Tomohiro
T
Iwano
Megumi
M
Tarutani
Yoshiaki
Y
Watanabe
Masao
M
Isogai
Akira
A
Takayama
Seiji
S
D001937:Brassica / Q000235:genetics*; D019175:DNA Methylation; D018744:DNA, Plant / Q000235:genetics*; D005799:Genes, Dominant; D006023:Glycoproteins / Q000235:genetics*; D007178:Inbreeding; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D011058:Pollen / Q000235:genetics*; D012098:Reproduction / Q000235:genetics
D018744:DNA, Plant; D006023:Glycoproteins; D010940:Plant Proteins; C064156:self-incompatibility locus specific glycoprotein, Brassica