Isolation of Rickettsia felis in the mosquito cell line C6/36.
We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene).
Horta
Maurício C
MC
Faculty of Veterinary Medicine, University of Sao Paulo, São Paulo, SP 05508-270, Brazil.
Labruna
Marcelo B
MB
Durigon
Edison L
EL
Schumaker
Teresinha T S
TT
D000330:Aedes / Q000382:microbiology*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001431:Bacteriological Techniques; D002460:Cell Line; D005798:Genes, Bacterial; D041161:Rickettsia felis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
D001425:Bacterial Outer Membrane Proteins