title: Antibacterial activity of cefquinome against equine bacterial pathogens.
abstract: Cefquinome is known for its use as an antibacterial drug in cattle and pigs. The objective of this study was to evaluate the antibacterial activity of cefquinome against equine pathogenic bacteria. The minimum inhibitory concentration (MIC) of cefquinome was determined for a total of 205 strains, which had recently been isolated in Europe from diseased horses (respiratory infection, foal septicaemia). The bactericidal activity was tested against 19 strains using the time killing method. The post-antibiotic effect (PAE) and post-antibiotic sub-MIC effect (PA SME) were determined against 12 strains. Cefquinome showed high activity against Actinobacillus equuli and streptococci (MIC(90) of 0.016 and 0.032microg/mL), Enterobacteriaceae (MIC(90)=0.125microg/mL) and staphylococci (MIC(90)=0.5microg/mL). The activity was limited against Rhodococcus spp. and Pseudomonas spp. Cefquinome was shown to be a time dependent bactericidal antibiotic against the target pathogens, killing occurring at a concentration close to the MIC. A PAE of 0.5-10h was calculated against streptococci whereas no PAE was observed for Escherichia coli. A longer PA SME was determined for streptococci (3.3 to >24h with a killing effect) and E. coli (0.5-13.9h). Cefquinome was shown to have a broad spectrum of activity which covers many equine pathogens.
authors.affiliation: Intervet Innovation GmbH, Zur Propstei, D-55270 Schwabenheim, Germany. emmanuel.thomas@intervet.com
authors.forename: E
authors.initials: E
authors.lastname: Thomas
authors.forename: V
authors.initials: V
authors.lastname: Thomas
authors.forename: C
authors.initials: C
authors.lastname: Wilhelm
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001424:Bacterial Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D002511:Cephalosporins / Q000494:pharmacology*; D015169:Colony Count, Microbial / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D006734:Horse Diseases / Q000188:drug therapy* / Q000382:microbiology*; D006736:Horses; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; C068212:cefquinome