title: An ELISA method for the detection and quantification of human heparanase.
abstract: Heparanase is a mammalian endo-beta-D-glucuronidase that cleaves heparan sulfate side chains at a limited number of sites. Heparanase enzymatic activity is thought to participate in degradation and remodeling of the extracellular matrix and to facilitate cell invasion associated with tumor metastasis, angiogenesis, and inflammation. Traditionally, heparanase activity was well correlated with the metastatic potential of a large number of tumor-derived cell types. More recently, heparanase upregulation was detected in an increasing number of primary human tumors, correlating, in some cases, with poor postoperative survival and increased tumor vascularity. The present study was undertaken to develop a highly sensitive ELISA suitable for the determination and quantification of human heparanase in tissue extracts and body fluids. The assay preferentially detects the 8+50 kDa active heparanase heterodimer vs. the latent 65 kDa proenzyme and correlates with immunoblot analysis of heparanase containing samples. It detects heparanase at concentrations as low as 200 pg/ml and is suitable for quantification of heparanase in tissue extracts and urine.
authors.affiliation: Cancer and Vascular Biology Research Center, The Bruce Rappaport Faculty of Medicine, Technion, Haifa 31096, Israel.
authors.forename: Itay
authors.initials: I
authors.lastname: Shafat
authors.forename: Eyal
authors.initials: E
authors.lastname: Zcharia
authors.forename: Benjamin
authors.initials: B
authors.lastname: Nisman
authors.forename: Yona
authors.initials: Y
authors.lastname: Nadir
authors.forename: Farid
authors.initials: F
authors.lastname: Nakhoul
authors.forename: Israel
authors.initials: I
authors.lastname: Vlodavsky
authors.forename: Neta
authors.initials: N
authors.lastname: Ilan
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002295:Carcinoma, Transitional Cell / Q000201:enzymology; D003928:Diabetic Nephropathies / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005966:Glucuronidase / Q000032:analysis* / Q000276:immunology / Q000652:urine; D006801:Humans; D007938:Leukemia / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D051379:Mice; D001749:Urinary Bladder Neoplasms / Q000201:enzymology; D000099175:Heparanase
chemical_list: D015415:Biomarkers; D005966:Glucuronidase; D000099175:Heparanase