[Enzymatic degradation of organophosphorus insecticide chlorpyrifos by fungus WZ-I].
Degradation characteristics of chlorpyrifos insecticides was determined by the crude enzyme extracted from the isolated strain WZ-I ( Fusarium LK. ex Fx). The best separating condition and the degrading characteristic of chlorpyrifos were studied. Rate of degradation for chlorpyrifos by its intracellular enzyme, extracellular enzyme and cell fragment was 60.8%, 11.3% and 48%, respectively. The degrading enzyme was extracted after this fungus was incubated for 8 generations in the condition of noninducement, and its enzymic activity lost less, the results show that this enzyme is an intracellular and connatural enzyme. The solubility protein of the crude enzyme was determined with Albumin (bovine serum) as standard protein and the solubility protein of the crude enzyme was 3.36 mg x mL(-1). The pH optimum for crude enzyme was 6.8 for enzymatic degradation of chlorpyrifos, and it had comparatively high activity in the range of pH 6.0 - 9.0. The optimum temperature for enzymatic activity was at 40 degrees C, it still had comparatively high activity in the range of temperature 20-50 degrees C, the activity of enzyme rapidly reduced at 55 degrees C, its activity was 41% of the maximal activity. The crude enzyme showed Km value for chlorpyrifos of 1.049 26 mmol x L(-1), and the maximal enzymatic degradation rate was 0.253 5 micromol x (mg x min)(-1). Additional experimental evidence suggests that the enzyme had the stability of endure for temperature and pH, the crude enzyme of fungus WZ-I could effectively degrade chlorpyrifos.
Xie
Hui
H
College of Resources and Environment, Shandong Agricultural University, Taian 271018, China. eti@sdau.edu.cn
Zhu
Lu-sheng
LS
Wang
Jun
J
Wang
Xiu-guo
XG
Liu
Wei
W
Qian
Bo
B
Wang
Qian
Q
D001673:Biodegradation, Environmental; D004390:Chlorpyrifos / Q000378:metabolism*; D005658:Fungi / Q000145:classification / Q000201:enzymology / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*
D007306:Insecticides; D009943:Organophosphorus Compounds; D004390:Chlorpyrifos