Two new colorimetric methods for early detection of vancomycin and oxacillin resistance in Staphylococcus aureus.
We developed two colorimetric methods for the detection of vancomycin- and oxacillin-resistant Staphylococcus aureus in </=6 h: (i) a nitrate reductase assay and (ii) a resazurin microplate method. MICs agreed with results obtained by CLSI methods for oxacillin. However, detection of vancomycin resistance required a larger inoculum. These methods may be recommended for the detection of vancomycin- and oxacillin-resistant S. aureus.
Coban
Ahmet Y
AY
Department of Pathology, Hershey Medical Center, P.O. Box 850, Hershey, PA 17033, USA.
Bozdogan
Bülent
B
Cihan
Cigdem Cekic
CC
Cetinkaya
Ebru
E
Bilgin
Kemal
K
Darka
Ozge
O
Akgunes
Alper
A
Durupinar
Belma
B
Appelbaum
Peter C
PC
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003124:Colorimetry / Q000379:methods; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D050901:Nitrate Reductase / Q000378:metabolism*; D010068:Oxacillin / Q000494:pharmacology*; D010078:Oxazines / Q000378:metabolism*; D010403:Penicillin Resistance; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism; D020713:Vancomycin Resistance; D014966:Xanthenes / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D050901:Nitrate Reductase; D010068:Oxacillin