Plasma urocortin 1 in sheep: regional sampling and effects of experimental heart failure.
Although urocortin 1 (Ucn-1) has been reported to circulate in human plasma and be raised in heart failure, little, if any, information is available regarding the source of circulating Ucn-1. Accordingly, we have performed trans-organ arteriovenous sampling for measurement of Ucn-1 concentration in anesthetized sheep before and after development of pacing-induced heart failure. Arterial plasma Ucn-1 levels measured 15.2 +/- 0.5 pmol/L in normal sheep and increased significantly following development of heart failure to 19.1 +/- 1.6 (p < 0.05). Small but significant positive arteriovenous gradients were observed across the hepatic and renal tissue beds in both states, with rises across the hind limb significant in normal animals and across the head in heart failure. This is the first report identifying sources of circulating Ucn-1.
Charles
Christopher J
CJ
Christchurch Cardioendocrine Research Group, Christchurch School of Medicine and Health Sciences, Christchurch, New Zealand. chris.charles@chmeds.ac.nz
Rademaker
Miriam T
MT
Richards
A Mark
AM
Yandle
Timothy G
TG
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003346:Corticotropin-Releasing Hormone / Q000097:blood* / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006333:Heart Failure / Q000097:blood*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D051381:Rats; D012756:Sheep; D054832:Urocortins
D010455:Peptides; D054832:Urocortins; D003346:Corticotropin-Releasing Hormone