Parallel picoliter rt-PCR assays using microfluidics.
The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis.
Marcus
Joshua S
JS
Option in Biochemistry and Molecular Biophysics, Department of Applied Physics, California Institute of Technology, Pasadena, 91125, USA.
Anderson
W French
WF
Quake
Stephen R
SR
D006801:Humans; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D012313:RNA / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity
D012313:RNA