Identification of post-transcriptionally regulated Xenopus tropicalis maternal mRNAs by microarray.
Cytoplasmic control of the adenylation state of mRNAs is a critical post-transcriptional process involved in the regulation of mRNAs stability and translational efficiency. The early development of Xenopus laevis has been a major model for the study of such regulations. We describe here a microarray analysis to identify mRNAs that are regulated by changes in their adenylation state during oogenesis and early development of the diploid frog Xenopus tropicalis. The microarray data were validated using qRT-PCR and direct analysis of the adenylation state of endogenous maternal mRNAs during the period studied. We identified more than 500 mRNAs regulated at the post-transcriptional level among the 3000 mRNAs potentially detected by the microarray. The mRNAs were classified into nine different adenylation behavior categories. The various adenylation profiles observed during oocyte maturation and early development and the analyses of 3'-untranslated region sequences suggest that previously uncharacterized sequence elements control the adenylation behavior of the newly identified mRNAs. These data should prove useful in identifying mRNAs with important functions during oocyte maturation and early development.
Graindorge
Antoine
A
CNRS UMR 6061, IFR 140, Functional Genetics, Agricultural and Health Sciences, Faculté de Médecine, Université de Rennes, 1 Rennes, France.
Thuret
Raphaël
R
Pollet
Nicolas
N
Osborne
H Beverley
HB
Audic
Yann
Y
D020413:3' Untranslated Regions / Q000737:chemistry; D000818:Animals; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008540:Meiosis; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D009866:Oogenesis; D026723:Polyadenylation; D039104:RNA 3' Polyadenylation Signals; D012333:RNA, Messenger / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014981:Xenopus
D020413:3' Untranslated Regions; D012333:RNA, Messenger