Probing in vivo promoter activities in Mycoplasma pneumoniae: a system for generation of single-copy reporter constructs.
The nucleotide sequences that control transcription initiation and regulation in Mycoplasma pneumoniae are poorly understood. In this work, we developed a lacZ-based reporter plasmid that can be used to integrate fusions of promoter fragments to a promoterless lacZ gene into the chromosome of M. pneumoniae.
Halbedel
Sven
S
Department of General Microbiology, Institute of Microbiology and Genetics, Georg-August University Göttingen, Grisebachstr. 8, D-37077 Göttingen, Germany.
Stülke
Jörg
J
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D050939:Gene Fusion; D005798:Genes, Bacterial; D017930:Genes, Reporter; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D007763:Lac Operon; D009177:Mycoplasma pneumoniae / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic
D004269:DNA, Bacterial; D007770:L-Lactate Dehydrogenase