High-throughput multi-antigen microfluidic fluorescence immunoassays.
Here we describe the development of a high-throughput multi-antigen microfluidic fluorescence immunoassay system. A 100-chamber polydimethylsiloxane (PDMS) chip performs up to 5 tests for each of 10 samples. In this particular study system, the specificity of detection was demonstrated, and calibration curves were produced for C-reactive protein (CRP), prostate-specific antigen (PSA), ferritin, and vascular endothelial growth factor (VEGF). The measurements show sensitivity at and below clinically normal levels (with a signal-to-noise ratio >8 at as low as 10 pM antigen concentration). The chip uses 100 nL per sample for all tests. The developed system is an important step toward derivative immunoassay applications in scientific research and "point-of-care" testing in medicine.
Kartalov
Emil P
EP
Dept of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles 90033, USA. kartalov@usc.edu
Zhong
Jiang F
JF
Scherer
Axel
A
Quake
Stephen R
SR
Taylor
Clive R
CR
Anderson
W French
WF
D000941:Antigens / Q000032:analysis*; D002097:C-Reactive Protein / Q000032:analysis; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D005293:Ferritins / Q000097:blood; D015200:Fluoroimmunoassay / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization; D017430:Prostate-Specific Antigen / Q000097:blood; D012828:Silicones / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
D000941:Antigens; D004129:Dimethylpolysiloxanes; D012828:Silicones; D042461:Vascular Endothelial Growth Factor A; C013830:baysilon; D002097:C-Reactive Protein; D005293:Ferritins; D017430:Prostate-Specific Antigen