Selection of an Escherichia coli O157:H7 bacteriophage for persistence in the circulatory system of mice infected experimentally.
A bacteriophage lytic for Escherichia coli O157:H7 was isolated from bovine manure. Following in-vivo selection, the phage acquired the capacity to persist in the circulatory system of mice for at least 38 days. When mice were infected experimentally with E. coli O157:H7 (10(7) CFU/mouse), simultaneous injection of the mice with phage (10(8) PFU/mouse) cleared E. coli O157:H7 from the mice within 48 h.
Capparelli
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School of Biotechnology, University of Naples 'Federico II', Naples, Italy.
Ventimiglia
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I
Roperto
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S
Fenizia
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D
Iannelli
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D
D000818:Animals; D003090:Coliphages / Q000302:isolation & purification* / Q000502:physiology; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000517:prevention & control; D019453:Escherichia coli O157 / Q000302:isolation & purification / Q000821:virology*; D005260:Female; D008242:Lysogeny; D008372:Manure / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014766:Viremia
D008372:Manure