title: Phage display selection of peptides that inhibit metastasis ability of gastric cancer cells with high liver-metastatic potential.
abstract: Organ-specific metastasis is an important character of cancer cells. Cancer cells that can metastasize to a special organ were thought to have different proteins in cell membrane, which might have potential utility as diagnostic markers and therapeutic targets. In the present work, based on high liver-metastatic gastric cancer cells, XGC9811-L, a screening approach with phage displayed peptide library, was successfully used to isolate 8-mer peptide ligands binding to the target cells. The phage20 had the highest binding efficiency to XGC9811-L cells, which also displayed remarkable cell specificity. Peptide20 that was displayed on phage20 could suppress the motility and invasion of XGC9811-L significantly. The adhesive ability of XGC9811-L to collagen IV was also inhibited by peptide20. Furthermore, phage20 could significantly reduce the incidence of liver metastasis of gastric cancer transplanted into nude mice and was also beneficial for the reduction the number of metastatic nodules in the liver. In conclusion, the phage display is an effective method to screen for the new molecules associated with organ-specific metastasis. The selected peptide20 can reverse the liver metastasis behavior of the gastric cancer cells.
authors.affiliation: State Key Laboratory of GI Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shanxi Province 710032, PR China.
authors.forename: Shengjuan
authors.initials: S
authors.lastname: Hu
authors.forename: Xinning
authors.initials: X
authors.lastname: Guo
authors.forename: Huahong
authors.initials: H
authors.lastname: Xie
authors.forename: Yulei
authors.initials: Y
authors.lastname: Du
authors.forename: Yanglin
authors.initials: Y
authors.lastname: Pan
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Shi
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Liu
authors.initials: L
authors.lastname: Hong
authors.forename: Shuang
authors.initials: S
authors.lastname: Han
authors.forename: Dongtao
authors.initials: D
authors.lastname: Zhang
authors.forename: Dawei
authors.initials: D
authors.lastname: Huang
authors.forename: Kedong
authors.initials: K
authors.lastname: Zhang
authors.forename: Feihu
authors.initials: F
authors.lastname: Bai
authors.forename: Haiping
authors.initials: H
authors.lastname: Jiang
authors.forename: Huihong
authors.initials: H
authors.lastname: Zhai
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Nie
authors.forename: Kaichun
authors.initials: K
authors.lastname: Wu
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001435:Bacteriophages / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary*; D051379:Mice; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D009362:Neoplasm Metastasis / Q000188:drug therapy*; D019151:Peptide Library; D010455:Peptides / Q000627:therapeutic use*; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D019151:Peptide Library; D010455:Peptides