Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S-adenosylmethionine methyltransferase.
In this article, a mechanism of arsenite [As(III)]resistance through methylation and subsequent volatization is described. Heterologous expression of arsM from Rhodopseudomonas palustris was shown to confer As(III) resistance to an arsenic-sensitive strain of Escherichia coli. ArsM catalyzes the formation of a number of methylated intermediates from As(III), with trimethylarsine as the end product. The net result is loss of arsenic, from both the medium and the cells. Because ArsM homologues are widespread in nature, this microbial-mediated transformation is proposed to have an important impact on the global arsenic cycle.
Qin
Jie
J
Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, MI 48201, USA.
Rosen
Barry P
BP
Zhang
Yang
Y
Wang
Gejiao
G
Franke
Sylvia
S
Rensing
Christopher
C
D001151:Arsenic / Q000378:metabolism* / Q000494:pharmacology; D001152:Arsenicals / Q000378:metabolism*; D018053:Arsenites / Q000378:metabolism; D003001:Cloning, Molecular; D004351:Drug Resistance / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008658:Inactivation, Metabolic; D008745:Methylation; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012241:Rhodopseudomonas / Q000201:enzymology* / Q000235:genetics; D012436:S-Adenosylmethionine / Q000378:metabolism; D014158:Transcription, Genetic; D014835:Volatilization
D001152:Arsenicals; D018053:Arsenites; D012436:S-Adenosylmethionine; D008780:Methyltransferases; C009572:trimethylarsine; C015001:arsenite; D001151:Arsenic