title: High-throughput, functional screening of the anti-HIV-1 humoral response by an enzymatic nanosensor.
abstract: The impact of antibodies on the target's epitope conformation is a major determinant of HIV-1 neutralization and a potential contributor to disease progression. We explore here a conformation-sensitive enzymatic nanosensor for the high-throughput functional screening of human anti-HIV-1 antibodies in sera. When displaying a model epitope from a gp41 immunodominant region (Env residues from 579 to 613), the sensing signal quantitatively distinguishes between adaptive and non-adaptive antibody binding. By using this tool, we have identified IgG4 as the immunoglobulin subpopulation most efficient in the structural modification of the target epitope.
authors.affiliation: Institut de Biotecnologia i de Biomedicina and Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Ferraz
authors.forename: Anna
authors.initials: A
authors.lastname: Arís
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Martínez
authors.forename: Antonio
authors.initials: A
authors.lastname: Villaverde
mesh_terms: D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001666:Binding Sites, Antibody / Q000276:immunology; D015374:Biosensing Techniques; D000939:Epitopes / Q000276:immunology; D005260:Female; D015700:HIV Envelope Protein gp41 / Q000276:immunology; D015658:HIV Infections / Q000097:blood* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D008297:Male; D036103:Nanotechnology; D013329:Structure-Activity Relationship
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D015700:HIV Envelope Protein gp41; D007074:Immunoglobulin G