title: Ham's F-10 medium and Ham's F-10 medium plus vitamin E have protective effect against oxidative stress in human semen.
abstract: OBJECTIVE
  To investigate possible protective effects of vitamin E and Ham's F-10 medium (HF-10) on lipid peroxidation, apoptosis, motility, and vitality of spermatozoa.
  
  
  METHODS
  Normozoospermic semen samples were obtained from 35 volunteers. Normal saline solution, HF-10 only, or HF-10 with vitamin E were added to split semen samples (control, group 1, and group 2, respectively). Sperm motility and vitality were evaluated at the end of 1, 2, and 24 hours. Superoxide dismutase, catalase, and malondialdehyde levels were assessed at the end of the first hour. Flow cytometric DNA analysis was performed at the end of 24 hours.
  
  
  RESULTS
  Superoxide dismutase, sperm motility, and vitality were not different among the groups. The catalase values decreased in group 1, but not in group 2. Malondialdehyde values in supernatants decreased in group 2 and apoptosis of spermatozoa decreased in groups 1 and 2.
  
  
  CONCLUSIONS
  Our data suggest that vitamin E and HF-10 protect against the reactive oxygen species-mediated damage on spermatozoa.
authors.affiliation: Department of Histology and Embryology, Karadeniz Technical University School of Medicine, Trabzon, Turkey. yenilmez@meds.ktu.edu.tr
authors.forename: Engin
authors.initials: E
authors.lastname: Yenilmez
authors.forename: Sermet
authors.initials: S
authors.lastname: Yildirmis
authors.forename: Esin
authors.initials: E
authors.lastname: Yulug
authors.forename: Sevim
authors.initials: S
authors.lastname: Aydin
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Tekelioglu
authors.forename: Erkan
authors.initials: E
authors.lastname: Erdem
authors.forename: Murat
authors.initials: M
authors.lastname: Topbas
authors.forename: Haluk
authors.initials: H
authors.lastname: Arvas
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D006801:Humans; D007552:Isotonic Solutions / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D012661:Semen; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism*; D014810:Vitamin E / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D007552:Isotonic Solutions; C083923:nutrient mixture F-10 Ham; D014810:Vitamin E