[Sequence analysis of a novel HLA-DRB1 allele, DRB1 * 1212].
OBJECTIVE
To investigate the molecular genetics basis for HLA novel allele HLA-DRB1*1212 in Chinese population.


METHODS
Genomic DNA was extracted from whole blood by salting-out method. HLA-DRB1 gene exon 2 was amplified by PCR with group-specific primers from genomic DNA. PCR products were cut back from agarose gels and purified to sequence directly. The polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSO) was performed to confirm the mutations which were detected by sequencing in this study.


RESULTS
The sequencing results showed HLA-DRB1 alleles of the proband as DRB1*090102 and the novel allele. The sequences of the novel allele have been submitted to GenBank (AY899825). Through BLAST analysis, the novel allele was found to be different from DRB1*120101 at position 199A-->C in exon 2, that results in an amino acid change from Ile to Leu at codon 67.


CONCLUSIONS
This allele is a novel and has been officially named as DRB1*1212 by the WHO Nomenclature Committee.
Zhu
Fa-ming
FM
Blood Center of Zhejiang Province, Hangzhou, Zhejiang 310006, P.R. China.
Zhang
Wei
W
Lu
Qin-feng
QF
He
Ji
J
Wang
Wei
W
Han
Zhe-dong
ZD
Yan
Li-xing
LX
D000483:Alleles; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D002681:China / Q000208:ethnology; D004247:DNA / Q000032:analysis*; D005006:Ethnicity; D005260:Female; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017421:Sequence Analysis
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C553515:HLA-DRB1*12:12 antigen; D004247:DNA