title: In vitro effect of zinc and cadmium on acetylcholinesterase and ectonucleotidase activities in zebrafish (Danio rerio) brain.
abstract: Zinc and cadmium are environmental contaminants that induce a wide range of effects on CNS. Here we tested the in vitro effect of these metals on acetylcholinesterase (AChE) and ectonucleotidase (NTPDase and ecto-5'-nucleotidase) activities in zebrafish brain. Both zinc and cadmium treatments did not alter significantly the zebrafish brain AChE activity. ATP hydrolysis presented a significant increase at 1 mM zinc (17%) and the AMPase activity had a dose-dependent increase at 0.5 and 1 mM zinc exposure (188% and 199%). After cadmium treatment, ATPase activity was significantly increased (53% and 48%) at 0.5 and 1 mM, respectively. Cadmium, in the range 0.25-1 mM, inhibited ADP hydrolysis in a dose-dependent manner (13.4-69%). Ecto-5'-nucleotidase activity was only inhibited (38%) in the presence of 1 mM cadmium. It is possible to suggest that changes on NTPDase and ecto-5'-nucleotidase activities can be an important mechanism involved in neurotoxic effects promoted by zinc and cadmium.
authors.affiliation: Laboratório de Pesquisa Bioquímica, Departamento de Ciências Fisiológicas, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga, 6681, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Mario Roberto
authors.initials: MR
authors.lastname: Senger
authors.forename: Denis Broock
authors.initials: DB
authors.lastname: Rosemberg
authors.forename: Eduardo Pacheco
authors.initials: EP
authors.lastname: Rico
authors.forename: Marcelo
authors.initials: M
authors.lastname: de Bem Arizi
authors.forename: Renato Dutra
authors.initials: RD
authors.lastname: Dias
authors.forename: Maurício Reis
authors.initials: MR
authors.lastname: Bogo
authors.forename: Carla Denise
authors.initials: CD
authors.lastname: Bonan
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000110:Acetylcholinesterase / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002104:Cadmium / Q000633:toxicity*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009708:Nucleotidases / Q000378:metabolism; D015027:Zebrafish; D015032:Zinc / Q000633:toxicity*
chemical_list: D002104:Cadmium; D000110:Acetylcholinesterase; D009708:Nucleotidases; C018775:adenosine monophosphatase; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases; D015032:Zinc