Expression of cyclooxygenase-1 and cyclooxygenase-2 in human renal allograft rejection-- a prospective study.
Cyclooxygenases (COX) are known to be involved in inflammatory kidney diseases. However, there are no data available about the expression of COX-1 and only preliminary reports about the expression of COX-2 in biopsies of patients undergoing acute renal allograft rejection. We conducted this prospective study to analyze the expression, distribution, and cellular localization of COX-1 and -2 and thus to elucidate the role of COX in human kidney transplantation. One hundred forty-four biopsies were included from patients without rejection and unaltered morphology (n = 60), with acute interstitial rejection (n = 7), with acute vascular rejection (n = 21), with chronic allograft nephropathy (n = 16), without rejection but with various other lesions (n = 40). COX-1 and -2 expression was localized in each biopsy by immunohistochemistry. We found a highly significant up-regulation of COX-1 in vessels and in infiltrating interstitial cells of patients with acute allograft rejection compared with biopsies with well-preserved tissue. Also, COX-2 expression was significantly elevated in infiltrating interstitial cells of biopsies with acute rejection. This is the first prospective study demonstrating a significant induction of both COX-1 and -2 in human allograft biopsies with acute rejection after renal transplantation.
Hoffmann
Ute
U
Klinik und Poliklinik für Innere Medizin II, University of Regensburg, Germany. ute.hoffmann@klinik.uni-regensburg.de
Banas
Bernhard
B
Krüger
Bernd
B
Pietrzyk
Miriam
M
Obed
Aiman
A
Segerer
Stephan
S
Kammerl
Martin
M
Rümmele
Petra
P
Riegger
Günter A J
GA
Krämer
Bernhard K
BK
D000328:Adult; D000368:Aged; D001161:Arteriosclerosis / Q000473:pathology; D001706:Biopsy; D051545:Cyclooxygenase 1 / Q000096:biosynthesis*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D005260:Female; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D006084:Graft Rejection; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation
D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2