title: Peptide sequence prediction supported by correlation-associated networks in human cerebrospinal fluid.
abstract: During the course of biosynthesis, processing and degradation of a peptide, many structurally related intermediate peptide products are generated. Human body fluids and tissues contain several thousand peptides that can be profiled by reversed-phase chromatography and subsequent MALDI-ToF-mass spectrometry. Correlation-Associated Peptide Networks (CAN) efficiently detect structural and biological relations of peptides, based on statistical analysis of peptide concentrations. We combined CAN with recognition of probable cleavage sites for peptidases and proteases in cerebrospinal fluid, resulting in a model able to predict the sequence of unknown peptides with high accuracy. On the basis of this approach, identification of peptide coordinates can be prioritized, and a rapid overview of the peptide content of a novel sample source can be obtained.
authors.affiliation: Department of Bioinformatics, BioVisioN AG, Feodor-Lynen-Str. 5, D-30625 Hannover, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Lamerz
authors.forename: Reto
authors.initials: R
authors.lastname: Crameri
authors.forename: Leonardo
authors.initials: L
authors.lastname: Scapozza
authors.forename: Thomas
authors.initials: T
authors.lastname: Mohring
authors.forename: Hartmut
authors.initials: H
authors.lastname: Selle
authors.forename: Hans-Dieter
authors.initials: HD
authors.lastname: Zucht
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D005260:Female; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation / Q000502:physiology*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D016511:Severe Combined Immunodeficiency / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D019151:Peptide Library; D010455:Peptides