Strain typing with ISLpl1 in lactobacilli.
Twenty-seven Lactobacillus plantarum ssp. plantarum, 11 Lactobacillus paraplantarum and five Lactobacillus casei-related strains, isolated from various autochthonous Serbian and Montenegro-fermented foods, were identified using phenotypical characterization and current PCR methods based on PCR of the recA gene or the 23S-5S rRNA gene intragenic spacer (IS) region. The strains were genotypically characterized by a new method based on the insertion sequence element ISLpl11 that grouped these lactobacilli into 10 IS-fingerprinting groups. Between six and 23 copies of the ISLpl1 were found in each strain and the ISLpl1-fingerprint groups correlated well with the origin of the strains. The method proved suitable for strain typing of lactic acid bacteria at the infraspecies level.
Petrovic
Tanja
T
Institute of Food Technology and Biotechnology, Faculty of Agriculture, University of Belgrade, Serbia and Montenegro.
Niksic
Miomir
M
Bringel
Françoise
F
D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000032:analysis*
D004251:DNA Transposable Elements; D012336:RNA, Ribosomal, 16S