Genotoxicity and cytotoxicity of mineral trioxide aggregate and regular and white Portland cements on Chinese hamster ovary (CHO) cells in vitro.
OBJECTIVE
Recently, mineral trioxide aggregate (MTA) and Portland cement have been used in dentistry as root-end-filling materials. However, the reported results concerning the biocompatibility of these materials are inconsistent. The goal of this study was to examine the genotoxicity and cytotoxicity of MTA and Portland cements in vitro by the single-cell gel (comet) assay and trypan blue exclusion test.


METHODS
Chinese hamster ovary (CHO) cells were exposed to MTA and regular and white Portland cements at final concentration ranging from 1 to 1000 microg/mL for 1 h at 37 degrees C.


RESULTS
All compounds tested did not show genotoxic effects in all concentrations evaluated. No significant differences (P > .05) in cytotoxicity were observed for all compounds tested.


CONCLUSIONS
Taken together, our results suggest that MTA and Portland cements are not genotoxins and are not able to induce cellular death.
Ribeiro
Daniel Araki
DA
Department of Pathology, Botucatu Medical School, Botucatu, Brazil. ak92@hotmail.com
Sugui
Marina Mariko
MM
Matsumoto
Mariza Akemi
MA
Duarte
Marco Antonio Húngaro
MA
Marques
Mariangela Esther Alencar
ME
Salvadori
Daisy Maria Favero
DM
D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D016466:CHO Cells / Q000187:drug effects; D017610:Calcium Compounds / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D003738:Dental Cements / Q000633:toxicity; D004338:Drug Combinations; D010087:Oxides / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity; D018709:Statistics, Nonparametric; D014343:Trypan Blue
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004396:Coloring Agents; D003738:Dental Cements; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C472247:accelerated Portland cement; C086631:mineral trioxide aggregate; D014343:Trypan Blue