Molecular epidemiology of Mycobacterium tuberculosis in China: a nationwide random survey in 2000.
OBJECTIVE
To determine the contribution of the Mycobacterium tuberculosis (TB) Beijing genotype to the population structure of M. tuberculosis complex in China.


METHODS
Genotypes of 441 mycobacterial isolates were determined by spoligotyping and IS6110 restriction fragment length polymorphism (RFLP). The isolates were from a nationwide random survey for the epidemiology of TB conducted in 2000, and the drug susceptibility patterns and epidemiological data were known.


RESULTS
A total of 408 samples contained M. tuberculosis as determined by spoligotyping. Of the M. tuberculosis strains, 64.9% (265/408) were of the Beijing genotype. Using the chi-squared test, no statistically significant differences were observed in the proportion of Beijing genotype TB in patients of different sex, age or living in different areas of the country, but a significant difference was observed with multidrug-resistant (MDR) TB: 77.8% (42/ 54) of MDR isolates were of the Beijing genotype vs. 60.2% (213/354) of the drug-susceptible isolates. In the multivariate logistic regression model, the Beijing genotype was significantly associated with region and not with MDR.


CONCLUSIONS
The M. tuberculosis Beijing genotype has prevailed in China for at least five decades and is associated with region.
Li
W M
WM
Department of Bacteriology and Immunology, Beijing Tuberculosis and Thoracic Tumour Research Institute, Beijing People's Republic of China.
Wang
S M
SM
Li
C Y
CY
Liu
Y H
YH
Shen
G M
GM
Zhang
X X
XX
Niu
T G
TG
Gao
Q
Q
van Soolingen
D
D
Kremer
K
K
Duanmu
H J
HJ
D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D015373:Bacterial Typing Techniques; D002648:Child; D002681:China / Q000453:epidemiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005838:Genotype; D006306:Health Surveys; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D017678:Sex Distribution; D014376:Tuberculosis / Q000453:epidemiology*
D004269:DNA, Bacterial