Biosynthesis of 14C-phytoene from tomato cell suspension cultures (Lycopersicon esculentum) for utilization in prostate cancer cell culture studies.
This work describes the development and utilization of a plant cell culture production approach to biosynthesize and radiolabel phytoene and phytofluene for prostate cancer cell culture studies. The herbicide norflurazon was added to established cell suspension cultures of tomato (Lycopersicon esculentum cv. VFNT cherry), to induce the biosynthesis and accumulation of the lycopene precursors, phytoene and phytofluene, in their natural isomeric forms (15-cis-phytoene and two cis-phytofluene isomers). Norflurazon concentrations, solvent carrier type and concentration, and duration of culture exposure to norflurazon were screened to optimize phytoene and phytofluene synthesis. Maximum yields of both phytoene and phytofluene were achieved after 7 days of treatment with 0.03 mg norflurazon/40 mL fresh medium, provided in 0.07% solvent carrier. Introduction of 14C-sucrose to the tomato cell culture medium enabled the production of 14C-labeled phytoene for subsequent prostate tumor cell uptake studies. In DU 145 prostate tumor cells, it was determined that 15-cis-phytoene and an oxidized product of phytoene were taken up and partially metabolized by the cells. The ability to biosynthesize, radiolabel, and isolate these carotenoids from tomato cell cultures is a novel, valuable methodology for further in vitro and in vivo investigations into the roles of phytoene and phytofluene in cancer chemoprevention.
Campbell
Jessica K
JK
Division of Nutritional Sciences, University of Illinois at Urbana--Champaign, 905 South Goodwin Avenue, Illinois 61801, USA.
Rogers
Randy B
RB
Lila
Mary Ann
MA
Erdman
John W
JW
D000230:Adenocarcinoma / Q000378:metabolism; D002250:Carbon Radioisotopes; D002338:Carotenoids / Q000096:biosynthesis* / Q000378:metabolism; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D018551:Solanum lycopersicum / Q000378:metabolism*; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism*
D002250:Carbon Radioisotopes; D002338:Carotenoids; C003140:phytofluene; C100185:(all-E) phytoene