Flow injection chemiluminescent determination of amiodarone in pharmaceutical preparations using photogenerated tris(2,2'-bipyridyl)ruthenium(III).
A flow injection configuration was developed and evaluated for the chemiluminescent determination of amiodarone. The method is based on the reaction of the drug with tris(2,2'-bipyridyl)ruthenium(III), which was generated through the on-line photo-oxidation of tris(2,2'-bipyridyl)ruthenium(II) with peroxydisulfate. Under the optimum experimental conditions, a linear calibration graph was obtained over the range 3.0-60.0 microg ml(-1) with a detection limit of 0.28 microg ml(-1). The proposed method allows 120 injections h(-1) with excellent repeatability and precision (R.S.D. less than 0.5% and 2.8%, respectively) and a reagent consumption of only 0.37 micromol (0.27 mg) of Ru(bpy)(3)Cl(2) x 6H(2)O per determination. The method was successfully applied to the determination of amiodarone in commercial pharmaceutical formulations.
Pérez-Ruiz
Tomás
T
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia, Spain. tpr@um.es
Martínez-Lozano
Carmen
C
Martín
Jesús
J
Ruiz
Encarna
E
D000638:Amiodarone / Q000032:analysis*; D017022:Flow Injection Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity
D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D000638:Amiodarone