Effect of berberine on proliferation, biosynthesis of macromolecules, cell cycle and induction of intercalation with DNA, dsDNA damage and apoptosis in Ehrlich ascites carcinoma cells.
Our primary aim was to study berberine, a potential anti-cancer drug, for its cytotoxic and antiproliferative activity in-vitro using Ehrlich ascites carcinoma (EAC) cells. Cytotoxicity was measured by the growth inhibition assay. We investigated the effect of berberine on the biosynthesis of macro-molecules (DNA, RNA, proteins), cell cycle effects and induction of dsDNA damage and apoptosis in berberine-treated EAC cells. Our results showed that berberine acts cytotoxically on EAC cells. The cytotoxicity was directly concentration and time dependent. The highest cytotoxic concentrations (100 and 50 microg mL(-1)) induced intercalation of berberine with DNA, formation of dsDNA breaks, inhibition of DNA synthesis and death of EAC cells. A concentration of 10 mug mL(-1) induced clear apoptotic cell death, which was followed by inhibition of protein synthesis.
Letasiová
Silvia
S
Department of Biochemistry and Microbiology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-81237 Bratislava, Slovak Republic. silvia.letasiova@stuba.sk
Jantová
Sona
S
Miko
Milan
M
Ovádeková
Renáta
R
Horváthová
Mira
M
D000818:Animals; D017209:Apoptosis; D001599:Berberine / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor; D002453:Cell Cycle; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D011506:Proteins / Q000378:metabolism; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D011506:Proteins; D012334:RNA, Neoplasm; D001599:Berberine; D004247:DNA; C427203:calf thymus DNA