Real-time assay of immobilized tannase with a stopped-flow conductometric device.
A stopped-flow manifold was developed to assay and characterize immobilized tannase (EC 3.1.1.20). The immobilized enzyme reactor was inserted within the tube-type electrode pair (cell constant=103.2 cm(-1)) for a real-time conductometric measurement. Tris buffer (2 mM, pH=7.0) was used as the carrier for sensitivity improvement. The activities and kinetic parameters (Km values) for propyl gallate, methyl gallate and tannic acid were investigated.
Chang
Fu-Shiang
FS
Department of Bio-Industrial Mechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan.
Chen
Po-Chung
PC
Chen
Richie L C
RL
Lu
Fu-Ming
FM
Cheng
Tzong-Jih
TJ
D002265:Carboxylic Ester Hydrolases / Q000032:analysis* / Q000737:chemistry; D004553:Electric Conductivity; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000032:analysis* / Q000737:chemistry; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D005707:Gallic Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012680:Sensitivity and Specificity; D013634:Tannins / Q000737:chemistry; D013997:Time Factors
D004800:Enzymes, Immobilized; D013634:Tannins; D005707:Gallic Acid; D002265:Carboxylic Ester Hydrolases; C022711:tannase