[Influence of NTA on accumulation and subcellular distribution of copper and zinc in corn (Zea mays)].
The differential centrifugation method was used to study the subcellular distribution of Cu and Zn in the roots, stems and leaves of corn (zea mays) growing on multi-metal contaminated soil with the addition of chelator Nitrilotriacetic Acid (NTA). The results show that the subcellular distributions of Cu and Zn have significant relationship with the ability of metal uptake and accumulation in corn. NTA could evidently promote the uptake and accumulation of Cu and Zn in corn and affect on their distribution in cell wall and vacuole. Most of Cu was bound to the cell wall fraction and secondly cytoplasm fraction and only a small quantity of Cu bound to organelle fractions. For Zn, however, most of Zn was bound to cytoplasm fraction and there was more Zn bound to organelles than Cu. Under the inducement of NTA, there were increasing Cu and Zn bound to cytoplasm fraction, which mostly came from cell wall fraction and partly came from organelle fractions.
Zhou
Jian-min
JM
College of Environmental Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Dang
Zhi
Z
Tao
Xue-qin
XQ
Zhou
Yong-zhang
YZ
D003300:Copper / Q000378:metabolism*; D009571:Nitrilotriacetic Acid / Q000494:pharmacology*; D003313:Zea mays / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
D003300:Copper; D015032:Zinc; D009571:Nitrilotriacetic Acid