Reduced extinction of hippocampal-dependent memories in CPEB knockout mice.
CPEB is a sequence-specific RNA binding protein that regulates translation at synapses. In neurons of CPEB knockout mice, synaptic efficacy is reduced. Here, we have performed a battery of behavioral tests and find that relative to wild-type animals, CPEB knockout mice, although similar on many baseline behaviors, have reduced extinction of memories on two hippocampal-dependent tasks. A corresponding microarray analysis reveals that about 0.14% of hippocampal genes have an altered expression in the CPEB knockout mouse. These data suggest that CPEB-dependent local protein synthesis may be an important cellular mechanism underlying extinction of hippocampal-dependent memories.
Berger-Sweeney
Joanne
J
Department of Biology, Wellesley College, Wellesley, Massachusetts 02481, USA.
Zearfoss
N Ruth
NR
Richter
Joel D
JD
D000704:Analysis of Variance; D000818:Animals; D005108:Extinction, Psychological / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D018782:Maze Learning / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009474:Neurons / Q000378:metabolism; D040081:Protein Array Analysis; D014176:Protein Biosynthesis / Q000235:genetics / Q000502:physiology; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013037:Spatial Behavior / Q000502:physiology; D018709:Statistics, Nonparametric; D013569:Synapses / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors; D039221:mRNA Cleavage and Polyadenylation Factors
D016601:RNA-Binding Proteins; C490387:Cpeb1 protein, mouse; D014157:Transcription Factors; D039221:mRNA Cleavage and Polyadenylation Factors