title: [Chemical coupling of anti-dNA antibody on collagen coating].
abstract: OBJECTIVE
  To evaluate the feasibility and stability of chemically conjugating IgM on collagen films.
  
  
  METHODS
  IgM was labeled with 125I using the chloramine-T method. Six collagen films were randomly divided into two groups. In chemical coupling group 125I-labeled IgM was chemically coupled with the films through N-succinmiclyl-3- (2-pyridyl-dithio) propionate reaction. In control group 125I-labeled IgM was absorbed onto collagen films. The amount of IgM on the collagen films and the amount of IgM remained on the films after extensive rinsing with phosphate buffered saline were monitored by counting the radioactivity of 125I.
  
  
  RESULTS
  The amount of antibodies loaded onto collagen films in the chemical coupling group was 15 times higher than that on the control films, showing significant statistical difference (P < 0.01). And the stability of conjugation antibodies on collagen films was significantly better than the control films.
  
  
  CONCLUSIONS
  Chemical coupling is an effective approach to immobilize antibodies on collagen for further plasmid DNA tethering.
authors.affiliation: Key Laboratory of Biomaterials, Institute of Biomedical Engineering, CAMS and PUMC, Tianjin 300192, China.
authors.forename: Lan-xia
authors.initials: LX
authors.lastname: Liu
authors.forename: Xi-gang
authors.initials: XG
authors.lastname: Leng
authors.forename: Jing-yin
authors.initials: JY
authors.lastname: Yue
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Song
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.forename: Cun-xian
authors.initials: CX
authors.lastname: Song
mesh_terms: D015906:Angioplasty, Balloon, Coronary / Q000295:instrumentation; D000818:Animals; D000974:Antibodies, Antinuclear / Q000378:metabolism*; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D005822:Genetic Vectors; D007075:Immunoglobulin M / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D011485:Protein Binding; D015607:Stents; D013499:Surface Properties
chemical_list: D000974:Antibodies, Antinuclear; D020099:Coated Materials, Biocompatible; D007075:Immunoglobulin M; D003094:Collagen