title: How elevated pCO2 modifies total and metabolically active bacterial communities in the rhizosphere of two perennial grasses grown under field conditions.
abstract: The response of total (DNA-based analysis) and active (RNA-based analysis) bacterial communities to a pCO2 increase under field conditions was assessed using two perennial grasses: the nitrophilic Lolium perenne and the oligonitrophilic Molinia coerulea. PCR- and reverse transcriptase-PCR denaturing gradient gel electrophoresis analysis of 16S rRNA genes generated contrasting profiles. The pCO2 increase influenced mainly the active and root-associated component of the bacterial community. Bacterial groups responsive to the pCO2 increase were identified by sequencing of corresponding denaturing gradient gel electrophoresis bands. About 50% of retrieved sequences were affiliated to Proteobacteria. Our data suggest that Actinobacteria in soil and Myxococcales (Deltaproteobacteria) in root are stimulated under elevated pCO2.
authors.affiliation: Microbiology Laboratory, University of Neuchâtel, Neuchâtel, Switzerland. maryline.jossi@unine.ch
authors.forename: Maryline
authors.initials: M
authors.lastname: Jossi
authors.forename: Nathalie
authors.initials: N
authors.lastname: Fromin
authors.forename: Sonia
authors.initials: S
authors.lastname: Tarnawski
authors.forename: Florian
authors.initials: F
authors.lastname: Kohler
authors.forename: François
authors.initials: F
authors.lastname: Gillet
authors.forename: Michel
authors.initials: M
authors.lastname: Aragno
authors.forename: Jérôme
authors.initials: J
authors.lastname: Hamelin
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D017753:Ecosystem; D004586:Electrophoresis / Q000379:methods; D020459:Genes, rRNA; D008129:Lolium / Q000254:growth & development / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*; D006109:Poaceae / Q000254:growth & development / Q000382:microbiology*; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002245:Carbon Dioxide