title: mRNA-based parallel detection of active methanotroph populations by use of a diagnostic microarray.
abstract: A method was developed for the mRNA-based application of microbial diagnostic microarrays to detect active microbial populations. DNA- and mRNA-based analyses of environmental samples were compared and confirmed via quantitative PCR. Results indicated that mRNA-based microarray analyses may provide additional information on the composition and functioning of microbial communities.
authors.affiliation: Department of Bioresources, ARC Seibersdorf Research GmbH, A-2444 Seibersdorf, Austria. levente.bodrossy@arcs.ac.at
authors.forename: Levente
authors.initials: L
authors.lastname: Bodrossy
authors.forename: Nancy
authors.initials: N
authors.lastname: Stralis-Pavese
authors.forename: Marianne
authors.initials: M
authors.lastname: Konrad-Köszler
authors.forename: Alexandra
authors.initials: A
authors.lastname: Weilharter
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Reichenauer
authors.forename: David
authors.initials: D
authors.lastname: Schöfer
authors.forename: Angela
authors.initials: A
authors.lastname: Sessitsch
mesh_terms: D017753:Ecosystem; D004783:Environmental Microbiology; D005798:Genes, Bacterial; D008697:Methane / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D020560:Proteobacteria / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D008697:Methane