The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box.
Four AP2/EREBP genes encoding putative ethylene-responsive element binding factor (ERF)/AP2 domains were cloned from Brassica napus, and these genes could be induced by low temperature, ethylene, drought, high salinity, abscisic acid and jasmonate treatments. These four genes, named BnDREBIII-1 to BnDREBIII-4, were highly homologous and the 37th amino acid was the only difference among their ERF/AP2 domains. BnDREBIII-1 was demonstrated to be able to bind to both dehydration-responsive element and the GCC box and transactivate the expression of downstream genes, while BnDREBIII-4 could bind neither. Further results suggested that Ala37 might play a crucial role in the DNA binding or the stability of the ERF/AP2 domain.
Liu
Yang
Y
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Zhao
Tong-Jin
TJ
Liu
Jing-Mei
JM
Liu
Wei-Qun
WQ
Liu
Qiang
Q
Yan
Yong-Bin
YB
Zhou
Hai-Meng
HM
D000040:Abscisic Acid; D000222:Adaptation, Physiological / Q000235:genetics; D000409:Alanine; D001665:Binding Sites; D029688:Brassica napus / Q000235:genetics*; D017124:Conserved Sequence; D003517:Cyclopentanes; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D003681:Dehydration; D005030:Ethylenes; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000737:chemistry / Q000378:metabolism*; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D054883:Oxylipins; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D020218:Response Elements; D012492:Salts; D012639:Seeds / Q000235:genetics; D014157:Transcription Factors
D003517:Cyclopentanes; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D005030:Ethylenes; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D054883:Oxylipins; D010940:Plant Proteins; D012492:Salts; D014157:Transcription Factors; C011006:jasmonic acid; D000040:Abscisic Acid; C036216:ethylene; D000409:Alanine