title: Malic enzyme 1 genotype is associated with backfat thickness and meat quality traits in pigs.
abstract: Malic enzyme 1 (ME1) is a part of the tricarboxylate shuttle that provides NADPH and acetyl-CoA required in fatty acid biosynthesis. The pig ME1 locus maps on the proximal end of chromosome 1, where a quantitative trait loci (QTL) affecting fat deposition has been previously described. We amplified fragments of 1457 and 1459 bp that corresponded to the complete coding region and the 3'-untranslated region (UTR), respectively, of the pig ME1 gene. The sequences of these two fragments in pigs from three breeds (Landrace, Large White and Piétrain) contained five single nucleotide polymorphisms (SNP) in the 3'-UTR: C1706T, G1762T, A1807C, C1857A and T1880A. Three haplotypes were found in two generations of a selected Landrace population: H1 (C1706 G1762 A1807 C1857 A1880), H2 (C1706 G1762 A1807 C1857 T1880) and H3 (T1706 T1762 C1807 A1857 T1880). Using Bayesian association analyses, significant associations (highest posterior density at 95%) between ME1 genotype and backfat (BF) thickness at 171 days and muscular pH were found in a Landrace population.
authors.affiliation: Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. oriol.vidal@uab.es
authors.forename: O
authors.initials: O
authors.lastname: Vidal
authors.forename: L
authors.initials: L
authors.lastname: Varona
authors.forename: M A
authors.initials: MA
authors.lastname: Oliver
authors.forename: J L
authors.initials: JL
authors.lastname: Noguera
authors.forename: A
authors.initials: A
authors.lastname: Sànchez
authors.forename: M
authors.initials: M
authors.lastname: Amills
mesh_terms: D000818:Animals; D001823:Body Composition / Q000235:genetics*; D017931:DNA Primers; D005838:Genotype; D008291:Malate Dehydrogenase / Q000235:genetics*; D008460:Meat; D020641:Polymorphism, Single Nucleotide; D034421:Sus scrofa / Q000235:genetics* / Q000502:physiology
chemical_list: D017931:DNA Primers; D008291:Malate Dehydrogenase