title: Ca2+ oscillations induced by testosterone enhance neurite outgrowth.
abstract: Testosterone has short- and long-term roles in regulating neuronal function. Here, we show rapid intracellular androgen receptor-independent effects of testosterone on intracellular Ca2+ in neuroblastoma cells. We identified testosterone-induced Ca2+ signals that began primarily at the neurite tip, followed by propagation towards the nucleus, which was then repeated to create an oscillatory pattern. The initial transient depended upon production of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3], but subsequent transients required both extracellular Ca2+ influx and Ca2+ release from intracellular stores. Inhibition of pertussis toxin-sensitive G-protein receptors or the use of siRNA for the Ins(1,4,5)P3 receptor type 1 blocked the Ca2+ response, whereas inhibition or knock-down of the intracellular androgen receptor was without effect. Cytosolic and nuclear Ca2+ were buffered with parvalbumin engineered to be targeted to the cytosol or nucleus. Cytoplasmic parvalbumin blocked Ca2+ signaling in both compartments; nuclear parvalbumin blocked only nuclear signals. Expression of a mutant parvalbumin did not modify the testosterone-induced Ca2+ signal. Neurite outgrowth in neuroblastoma cells was enhanced by the addition of testosterone. This effect was inhibited when cytosolic Ca2+ was buffered and was attenuated when parvalbumin was targeted to the nucleus. Our results are consistent with a fast effect of testosterone, involving Ins(1,4,5)P3-mediated Ca2+ oscillations and support the notion that there is synergism in the pathways used for neuronal cell differentiation involving rapid non-genomic effects and the classical genomic actions of androgens.
authors.affiliation: Department of Pharmacology, Cell and Molecular Physiology, Yale University, New Haven, CT 06520, USA.
authors.forename: Manuel
authors.initials: M
authors.lastname: Estrada
authors.forename: Per
authors.initials: P
authors.lastname: Uhlen
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Ehrlich
mesh_terms: D020013:Calcium Signaling / Q000187:drug effects; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D016501:Neurites / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons / Q000502:physiology; D013739:Testosterone / Q000494:pharmacology / Q000502:physiology*
chemical_list: D013739:Testosterone; D015544:Inositol 1,4,5-Trisphosphate