title: Localization of a long-chain acyl-CoA hydrolase in spermatogenic cells in mice.
abstract: Brain acyl-CoA hydrolase (BACH) hydrolyzes long-chain acyl-CoAs to free fatty acids and CoA-SH. BACH is highly distributed in brain and is localized in neurons, but not glial cells. This suggests that BACH plays a specific role in neurons. BACH is also detected in testis, although the expression profile of BACH is unknown in testis. In this study, developmental changes and cellular distribution of BACH were examined in mouse testis. Before postnatal day (P) 10, BACH was detected at very low levels by Western blotting. Then, BACH content rapidly increased from P14 and reached maximum levels at P21, remaining high until at least P70. The increase in BACH content corresponded to the appearance of pachytene spermatocytes, which was confirmed by immunohistochemistry. BACH was also detectable in spermatids, but not in spermatogonia, mature spermatozoa. These results suggest that BACH is expressed in a cell-specific manner and plays a role in spermatogenesis.
authors.affiliation: Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Science, Tokyo, Japan. takagim@ps.toyaku.ac.jp
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Takagi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ohtomo
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hiratsuka
authors.forename: Yu
authors.initials: Y
authors.lastname: Kuramochi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Suga
authors.forename: Junji
authors.initials: J
authors.lastname: Yamada
mesh_terms: D000818:Animals; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010170:Palmitoyl-CoA Hydrolase / Q000378:metabolism*; D013087:Spermatids / Q000254:growth & development / Q000378:metabolism; D013090:Spermatocytes / Q000254:growth & development / Q000378:metabolism; D013091:Spermatogenesis; D013093:Spermatogonia / Q000254:growth & development / Q000378:metabolism; D013094:Spermatozoa / Q000254:growth & development / Q000378:metabolism*; D013737:Testis / Q000201:enzymology* / Q000254:growth & development
chemical_list: D010170:Palmitoyl-CoA Hydrolase