title: Periplasmic superoxide dismutase from Desulfovibrio desulfuricans 1388 is an iron protein.
abstract: It is shown that the genome of the sulfate-reducing bacterium Desulfovibrio desulfuricans 1388 contains a superoxide dismutase (SOD) gene (sod). The gene encodes an export signal peptide characteristic for periplasmic redox proteins. The amino acid sequence showed high homology with iron-containing SODs from other bacteria. Electrophoretically pure SOD was isolated from the periplasmic fraction of bacterial cells by FPLC chromatography. Like other Fe-SODs, D. desulfuricans 1388 superoxide dismutase is inhibited by H2O2 and azide, but not by cyanide.
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Research Center, Russian Academy of Sciences, Kazan, Russia. davydova@mail.knc.ru
authors.forename: M N
authors.initials: MN
authors.lastname: Davydova
authors.forename: O V
authors.initials: OV
authors.lastname: Gorshkov
authors.forename: N B
authors.initials: NB
authors.lastname: Tarasova
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D045285:Desulfovibrio desulfuricans / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008969:Molecular Sequence Data; D019897:Periplasm / Q000201:enzymology; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D007501:Iron; D013482:Superoxide Dismutase