Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: genotyping by MADGE.
OBJECTIVE
The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing.


METHODS
Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products.


RESULTS
PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis.


CONCLUSIONS
We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.
Zivković
Maja
M
Vinca Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics, PO Box 522, 11001 Belgrade, Serbia and Montenegro, UK. majaz@vin.bg.ac.yu
Raković
Aleksandar
A
Stanković
Aleksandra
A
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D011110:Polymorphism, Genetic
D017931:DNA Primers; D020780:Matrix Metalloproteinase 9