Measurement of serum interleukin-10 in the dog.
The objective was to evaluate independently the reliability of a commercially available canine serum interleukin-10 (IL-10) enzyme-linked immunoassay (ELISA) and to investigate canine serum IL-10 concentrations in healthy dogs, in dogs with a naturally-occurring acute phase reaction and in dogs following surgical stimulus by assessing intra- and interassay imprecision, inaccuracy and detection limits. Median (and range) serum IL-10 concentrations (ng/L) in the various groups were as follows: healthy dogs (n=15), 18.9 (11.2-71.5); dogs with pyometra (n=9), 37.9 (12.4-201.8); dogs with angiostrongylosis (n=8), 20.29 (14.3-108.7) and values in dogs following surgical stimulus (n=15), 14.8 (10.7-65.8). The assay measured canine serum IL-10 reliably (intra- and interassay imprecision 4.9-8.3% and 9.9-10.9%, respectively; detection limit 10.7 ng/L with no significant inaccuracy). No significant increases in IL-10 were observed following surgical stimulus and no difference in IL-10 was observed between the diagnostic groups. IL-10 values showed a higher degree of variation in dogs with an inflammatory response, i.e. those with elevated serum C-reactive protein (CRP) concentrations, compared to healthy dogs. As anticipated, healthy dogs had low levels of both analytes, whereas dogs with an acute phase response had IL-10 levels with no clear relationship to CRP concentrations, with observed low IL-10 values even when there was a marked inflammatory response.
Kjelgaard-Hansen
Mads
M
Central Laboratory, Department of Small Animal Clinical Sciences, The Royal Veterinary and Agricultural University, 3 Gronnegaardsvej, DK-1870 Frederiksberg, Denmark. mjkh@kvl.dk
Luntang-Jensen
Michael
M
Willesen
Jakob
J
Jensen
Asger L
AL
D000210:Acute-Phase Reaction / Q000097:blood; D000801:Angiostrongylus; D000818:Animals; D002097:C-Reactive Protein / Q000378:metabolism; D004285:Dogs / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D007249:Inflammation / Q000097:blood / Q000662:veterinary; D016753:Interleukin-10 / Q000097:blood*; D008297:Male; D017206:Strongylida Infections / Q000097:blood / Q000662:veterinary; D014591:Uterine Diseases / Q000097:blood / Q000662:veterinary
D016753:Interleukin-10; D002097:C-Reactive Protein