Evidence that IncG (IncP-6) and IncU plasmids form a single incompatibility group.
The Escherichia coli IncG and IncU plasmid incompatibility groups were assigned in 1980 and 1981, respectively. Complete plasmid sequences have recently been published for both these groups, and revealed that their replication proteins are related. We show that when cloned at high-copy-number, putative iterons from the previously identified IncG replicon cause strong incompatibility with IncU plasmids. Incompatibility, albeit weaker, was also demonstrated between the two replicons at their normal low-copy-number. This suggests that a single incompatibility group exists. The only known IncG plasmid, Rms149, can replicate in Pseudomonas species where it is designated IncP-6. We recommend that the combined group be known as IncU (IncP-6 in Pseudomonas spp.).
Haines
Anthony S
AS
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Cheung
Martin
M
Thomas
Christopher M
CM
D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D012093:Replicon
D004269:DNA, Bacterial