title: Evaluation of the immunochromatographic CORIS Giardia-Strip test for rapid diagnosis of Giardia lamblia.
abstract: The aim of this study was to evaluate the performance of the CORIS Giardia-Strip test (CORIS Bioconcept, Gembloux, Belgium) as a rapid initial method for the routine diagnosis of giardiasis. Compared to a commercial ELISA-coproantigen test (ProSpect Giardia-ELISA-microplate assay; Remel, Lenexa, KS, USA), the commercial strip test had a sensitivity of 58%, a specificity of 99%, a positive predictive value of 93% and a negative predictive value of 93% (n=158). These results are comparable to those obtained using microscopy of direct wet-mounted stool. Since the CORIS Giardia-Strip test is simpler to perform, it can replace direct wet-mounted stool microscopy for the rapid diagnosis of giardiasis; however, its sensitivity is inferior to that of other immunochromatographic antigen detection tests and fresh stool samples are required for its use. Nevertheless, the results suggest that a positive CORIS Giardia-Strip test outcome does not need confirmation, while samples with negative results should be re-examined using another, more sensitive, test.
authors.affiliation: Abteilung Parasitologie, Hygiene Institut, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Oster
authors.forename: H
authors.initials: H
authors.lastname: Gehrig-Feistel
authors.forename: H
authors.initials: H
authors.lastname: Jung
authors.forename: J
authors.initials: J
authors.lastname: Kammer
authors.forename: J E
authors.initials: JE
authors.lastname: McLean
authors.forename: M
authors.initials: M
authors.lastname: Lanzer
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000276:immunology / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D008853:Microscopy; D011237:Predictive Value of Tests; D011934:Reagent Strips; D012680:Sensitivity and Specificity
chemical_list: D000953:Antigens, Protozoan; D011934:Reagent Strips