Pharmacokinetics of Astragaloside IV in rats by liquid chromatography coupled with tandem mass spectrometry.
Pharmacokinetics of Astragaloside IV (AGS-IV) in rats was studied by high performance liquid chromatography coupled with tandem mass spectrometry. The concentration in plasma was determined after i.v. administration of 1, 2, 4 mg/kg and p.o. administration of 20 mg/kg of AGS-IV. The AUC were linearly correlated to doses. Recoveries of AGS-IV in bile, urine and feces were also analyzed following i.v. dose of 2 mg/kg. Cumulative recovery of AGS-IV in bile reached 30.8% in 24h. Cumulative recovery of AGS-IV in urine and feces was 52.14%, which indicates that about 50% of AGS-IV was metabolized in vivo. The bioavailability of AGS-IV after p.o. administration was found to be 3.66%. These findings provide useful information for the research and development of AGS-IV and other potential agents.
Du
Y
Y
College of Life and Pharmacy, Nanjing University of Technology, Nanjing, People's Republic of China.
Zhang
Q
Q
Chen
G G
GG
Wei
P
P
Tu
C Y
CY
D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001646:Bile / Q000737:chemistry; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005243:Feces / Q000737:chemistry; D007275:Injections, Intravenous; D013058:Mass Spectrometry; D008657:Metabolic Clearance Rate; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D014315:Triterpenes / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine
D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A