Studies on transcriptional regulation of endogenous genes by ERF2 transcription factor in tobacco cells.
In this study, we showed that overexpression of ethylene-responsive transcription factor (ERF) 2 activated the expression of endogenous genes that have the GCC box in their promoter region, in tobacco plants. These include not only a defense-related gene, CHN50, encoding class I basic chitinase, but also a transcriptional repressor gene, ERF3. In tobacco plants constitutively expressing ERF2:glucocorticoid receptor fusion protein, treatment with dexamethazone induced a rapid increase of ERF3 mRNA and a slow increase of CHN50 mRNA. These results suggest that an antagonistic interplay of ERF2 and ERF3 is involved in the transcriptional regulation of the class I basic chitinase genes in tobacco.
Nakano
Toshitsugu
T
Molecular and Cellular Breeding Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566 Japan.
Nishiuchi
Takumi
T
Suzuki
Kaoru
K
Fujimura
Tatsuhito
T
Shinshi
Hideaki
H
D002688:Chitinases / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D051790:G-Box Binding Factors / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000502:physiology; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology; D014026:Nicotiana / Q000235:genetics*; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
D018744:DNA, Plant; D051790:G-Box Binding Factors; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D012097:Repressor Proteins; D014157:Transcription Factors; D003907:Dexamethasone; D002688:Chitinases