Detection of early changes in sperm membrane integrity pre-freezing can estimate post-thaw quality of boar spermatozoa.
A recently developed triple staining (SNARF-1/YO-PRO-1/ethidium homodimer) was used to assess early changes in boar sperm membrane integrity (MI) with the results of cryopreservation procedures and to seek for correlations among MI-spermatozoa in pre-freeze semen and its freezeability. Ejaculates from five boars were evaluated in the fresh and frozen-thawed (FT) state, and its freezeability defined as % of membrane intactness, MI% (MI%=% of FT-spermatozoa with intact membranes x 100 divided by the % of pre-freeze spermatozoa with intact membranes) estimated. Significant differences were found among boars for freezeability (MI%) and motility post-thaw (%). Interestingly, significant correlations were found between the percentage of YO-PRO-1-positive spermatozoa and freezeability (R=0.440, p<0.01), indicating this new triple staining can be used to safely disclose among ejaculates prior to freezing.
Peña
F J
FJ
Section of Animal Reproduction, Department of Herd Health and Medicine, Faculty of Veterinary Medicine, University of Extremadura, Avd. de la Universidad s/n, 10071 Cáceres, Spain. fjuanpvega@unex.es
Saravia
F
F
Johannisson
A
A
Wallgren
M
M
Rodríguez-Martínez
H
H
D000818:Animals; D001583:Benzoxazoles / Q000378:metabolism; D002462:Cell Membrane / Q000648:ultrastructure*; D002463:Cell Membrane Permeability; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005456:Fluorescent Dyes; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods / Q000662:veterinary; D011806:Quinolinium Compounds / Q000378:metabolism; D012662:Semen Preservation / Q000009:adverse effects / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods / Q000662:veterinary; D013552:Swine
D001583:Benzoxazoles; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; C089813:YO-PRO 1