Metabolic engineering of coenzyme Q by modification of isoprenoid side chain in plant.
Coenzyme Q (CoQ), an electron transfer molecule in the respiratory chain and a lipid-soluble antioxidant, is present in almost all organisms. Most cereal crops produce CoQ9, which has nine isoprene units. CoQ10, with 10 isoprene units, is a very popular food supplement. Here, we report the genetic engineering of rice to produce CoQ10 using the gene for decaprenyl diphosphate synthase (DdsA). The production of CoQ9 was almost completely replaced with that of CoQ10, despite the presence of endogenous CoQ9 synthesis. DdsA designed to express at the mitochondria increased accumulation of total CoQ amount in seeds.
Takahashi
Sakiko
S
Department of Genetic Diversity, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Ogiyama
Yuki
Y
Kusano
Hiroaki
H
Shimada
Hiroaki
H
Kawamukai
Makoto
M
Kadowaki
Koh-ichi
K
D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D000975:Antioxidants / Q000502:physiology*; D001483:Base Sequence; D003067:Coenzymes; D018556:Crops, Agricultural / Q000201:enzymology / Q000235:genetics; D004579:Electron Transport / Q000502:physiology; D005818:Genetic Engineering / Q000379:methods; D008928:Mitochondria / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D030821:Plants, Genetically Modified; D012639:Seeds / Q000201:enzymology / Q000235:genetics*; D013729:Terpenes / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics
D000975:Antioxidants; D003067:Coenzymes; D013729:Terpenes; D014451:Ubiquinone; D019883:Alkyl and Aryl Transferases; C039821:decaprenyl pyrophosphate synthetase; C024989:coenzyme Q10; C030571:ubiquinone 9