An immunohistochemical study of protein kinase C in the bovine retina.
The expression of protein kinase C (PKC) was studied in the bovine retina by immunohistochemical analysis. Western blot analysis showed that PKC isoforms, including alpha, betaI, delta and theta, were detected in the bovine retina. By immunohistochemistry, both PKC alpha and betaI were expressed in all retinal layers, with an intense localization of both PKC alpha and betaI detected in bipolar cells in the inner nuclear cell layer and in some glial cells in ganglion cell layers. The immunoreactivity of both PKC delta and theta was quite weak in the retinal layers, compared with that of PKC alpha and betaI. These findings suggest that both conventional and novel PKCs are differentially expressed in the bovine retina.
Shin
Taekyun
T
Department of Veterinary Medicine, Graduate School, Cheju National University, Jeju city 690-756, Republic of Korea.
Kim
Seungjoon
S
Ahn
Meejung
M
Kim
Heechul
H
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D007150:Immunohistochemistry; D011493:Protein Kinase C / Q000378:metabolism*; D012160:Retina / Q000201:enzymology*
D011493:Protein Kinase C