SELDI-TOF MS of quadruplicate urine and serum samples to evaluate changes related to storage conditions.
Proteomic profiling with SELDI-TOF MS has facilitated the discovery of disease-specific protein profiles. However, multicenter studies are often hindered by the logistics required for prompt deep-freezing of samples in liquid nitrogen or dry ice within the clinic setting prior to shipping. We report high concordance between MS profiles within sets of quadruplicate split urine and serum samples deep-frozen at 0, 2, 6, and 24 h after sample collection. Gage R&R results confirm that deep-freezing times are not a statistically significant source of SELDI-TOF MS variability for either blood or urine.
Traum
Avram Z
AZ
Division of Nephrology, Children's Hospital Boston, Boston, MA, USA.
Wells
Meghan P
MP
Aivado
Manuel
M
Libermann
Towia A
TA
Ramoni
Marco F
MF
Schachter
Asher D
AD
D000418:Albumins / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis*; D015925:Cryopreservation / Q000379:methods; D015337:Multicenter Studies as Topic; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D014556:Urine / Q000737:chemistry*
D000418:Albumins; D001798:Blood Proteins