[Peculiarities of NO-syntase expression in experimental hypertension caused by inhibition during low frequency laser irradiation of cardiovascular system and kidneys].
The aim of our study was the investigation of expression of NO-syntase endothelial (eNOS) and inducted (iNOS) forms in cardiovascular system and kidneys at experimental hypertension caused by laser irradiation. For investigation of experimental model of hypertension the rats of Vister species together with the drinking water were administered by L-NAME, NO inhibitor. Rats were divided into the following groups: in group I, irradiation with laser was started immediately after L-NAME administration, in group II - on the third day, in group III - on the 12th day, but in group IV - on the 28th day, respectively. The animals have been killed on the 10th day after irradiation. The morphological and immunohistochemical studies of heart and blood vessels (coronary arteries, aorta and kidney artery) and kidneys have been performed, with determination of eNOS and iNOS expression. The obtained results show that at irradiation by laser irradiation of low-frequency immediately after administration of L-NAME, eNOS expression is kept as in heart and blood vessels as in kidneys then iNOS expression is started firstly in myocardium and kidney (II group) and then in all studied blood vessels (III group). The mentioned facts prove that the irradiation by a laser of low - frequency inhibits eNOS inhibition after L-NAME action that must represent the background of antihypertensic effect after laser irradiation. Due to the results of study, an irradiation by a laser of low - frequency might be used for prevention of hypertension in preclinical stage to avoid the further complications, but its using with the aim to relieve the clinical evidences is not recommended.
Mirziashvili
M G
MG
D000818:Animals; D002319:Cardiovascular System / Q000528:radiation effects*; D006973:Hypertension / Q000139:chemically induced / Q000201:enzymology*; D007668:Kidney / Q000528:radiation effects*; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage / Q000009:adverse effects; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D051381:Rats
D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester