title: Intranasal immunization with human papillomavirus type 16 capsomeres in the presence of non-toxic cholera toxin-based adjuvants elicits increased vaginal immunoglobulin levels.
abstract: Prophylactic immunization against human papillomaviruses (HPVs) aims preferentially at the generation of antibodies, which are directed against the virus capsid proteins. DNA-free virus-like particles or their pentameric subunits, the capsomeres represent suitable antigens. Here we investigated if anti-HPV16 L1 specific antibodies and L1-specific CTL induced by intranasal immunization with capsomeres in sera and vaginal washings of C57Bl6 mice can be enhanced by co-application of the non-toxic cholera toxin adjuvants CTA1-D2D1 or CTB. We found that CTA1-D2D1 elevated L1-specific serum IgG antibodies in a dose-dependent manner and both CTA1-D2D1 and CTB significantly increased L1-specific IgA antibody levels in the vaginal lumen. Furthermore, CTA1-D2D1 and CTB enhanced L1-specific CTL responses.
authors.affiliation: Deutsches Krebsforschungszentrum, Angewandte Tumorvirologie, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany. k.dell@dkfz.de
authors.forename: Kerstin
authors.initials: K
authors.lastname: Dell
authors.forename: Robert
authors.initials: R
authors.lastname: Koesters
authors.forename: Michael
authors.initials: M
authors.lastname: Linnebacher
authors.forename: Corinna
authors.initials: C
authors.lastname: Klein
authors.forename: Lutz
authors.initials: L
authors.lastname: Gissmann
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D036022:Capsid Proteins / Q000276:immunology*; D002772:Cholera Toxin / Q000008:administration & dosage*; D005260:Female; D052162:Human papillomavirus 16 / Q000276:immunology*; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014621:Vagina / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D036022:Capsid Proteins; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D009856:Oncogene Proteins, Viral; D014765:Viral Vaccines; C063317:L1 protein, Human papillomavirus type 16; D002772:Cholera Toxin