title: Comparative genomic organization of the human and bovine PRNP locus.
abstract: We sequenced a 208-kb BAC clone spanning the bovine prion protein (PRNP) locus, and compared the genomic structure with that of human. As a result, we determined the precise breakpoint between the two syntenic genomes, located on the 5' UTR of the PRNP gene, and discovered two highly repetitive sequences near the breakpoint. Further analysis demonstrated that the genomic structure of three genes, PRNP, PRND, and RASSF2, within the syntenic region of the bovine genome is highly conserved in order and orientation. The PRNT locus was not found in bovine but is conserved in several primates, including human. Moreover, we confirmed that the bovine RASSF2 is composed of 10 exons, as is the human gene, showing some difference from a previous report. Our findings may provide useful clues for understanding the evolutional process in the PRNP locus and also the mechanism that allows TSE from cattle to infect humans.
authors.affiliation: Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, 52, Oun-dong, Yusong-gu, Daejeon 305-333, Korea.
authors.forename: Sang-Haeng
authors.initials: SH
authors.lastname: Choi
authors.forename: Il-Chul
authors.initials: IC
authors.lastname: Kim
authors.forename: Dae-Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Dae-Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Sung-Hwa
authors.initials: SH
authors.lastname: Chae
authors.forename: Han-Ho
authors.initials: HH
authors.lastname: Choi
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
authors.forename: Jung-Sou
authors.initials: JS
authors.lastname: Yeo
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Song
authors.forename: Hong-Seog
authors.initials: HS
authors.lastname: Park
mesh_terms: D000682:Amyloid / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D022202:Chromosomes, Artificial, Bacterial; D058851:GPI-Linked Proteins; D006801:Humans; D008252:Macaca fascicularis / Q000235:genetics; D008969:Molecular Sequence Data; D011323:Primates / Q000235:genetics; D000072002:Prion Proteins; D011328:Prions / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics*; D011506:Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D026801:Synteny; D025521:Tumor Suppressor Proteins
chemical_list: D000682:Amyloid; D058851:GPI-Linked Proteins; C501125:PRND protein, human; C512656:PRNP protein, human; D000072002:Prion Proteins; D011328:Prions; D011498:Protein Precursors; D011506:Proteins; C425300:RASSF2 protein, human; D025521:Tumor Suppressor Proteins