Induction of humoral immunity in response to immunization with recombinant Mycobacterium bovis BCG expressing the S1 subunit of Bordetella pertussis toxin.
Two recombinant Mycobacterium bovis BCG (rBCG) vaccine strains were developed for the expression of cytoplasmically located S1 subunit of pertussis toxin, with expression driven by the hsp60 promoter of M. bovis (rBCG/pPB10) or the pAN promoter of Mycobacterium paratuberculosis (rBCG/pPB12). Both strains showed stable expression of equivalent levels of recombinant S1 in vitro and induced long-term (up to 8 months) humoral immune responses in BALB/c mice, although these responses differed quantitatively and qualitatively. Specifically, rBCG/pPB12 induced markedly higher levels of IgG1 than did rBCG/pPB10, and mice immunized with the former strain developed specific long-term memory to S1, as indicated by the production of high levels of S1-specific IgG in response to a sublethal challenge with pertussis toxin 15 months after initial immunization. When considered in combination with previous studies, our data encourage further evaluation of rBCG as a potential means of developing a low-cost whooping cough vaccine based on defined antigens.
Medeiros
Marco A
MA
Laboratory of Recombinant Technology, Bio-Manguinhos, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. medeiros@bio.fiocruz.br
Armôa
Geraldo R G
GR
Dellagostin
Odir A
OA
McIntosh
Douglas
D
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001886:Bordetella pertussis / Q000276:immunology*; D004353:Drug Evaluation, Preclinical; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood*; D007156:Immunologic Memory; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics; D009163:Mycobacterium bovis / Q000235:genetics*; D037342:Pertussis Toxin / Q000276:immunology*; D010567:Pertussis Vaccine / Q000008:administration & dosage / Q000276:immunology*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014917:Whooping Cough / Q000097:blood / Q000276:immunology*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D010567:Pertussis Vaccine; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D037342:Pertussis Toxin