title: Cutting edge: novel human dendritic cell- and monocyte-attracting chemokine-like protein identified by fold recognition methods.
abstract: Chemokines play an important role in the immune system by regulating cell trafficking in homeostasis and inflammation. In this study, we report the identification and characterization of a novel cytokine-like protein, DMC (dendritic cell and monocyte chemokine-like protein), which attracts dendritic cells and monocytes. The key to the identification of this putative new chemokine was the application of threading techniques to its uncharacterized sequence. Based on our studies, DMC is predicted to have an IL-8-like chemokine fold and to be structurally and functionally related to CXCL8 and CXCL14. Consistent with our predictions, DMC induces migration of monocytes and immature dendritic cells. Expression studies show that DMC is constitutively expressed in lung, suggesting a potential role for DMC in recruiting monocytes and dendritic cells from blood into lung parenchyma.
authors.affiliation: Department of Protein Engineering, Genentech Inc., South Francisco, CA 94080, USA. mayte@biotec.tu-dresden.de
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Pisabarro
authors.forename: Beatrice
authors.initials: B
authors.lastname: Leung
authors.forename: Mandy
authors.initials: M
authors.lastname: Kwong
authors.forename: Racquel
authors.initials: R
authors.lastname: Corpuz
authors.forename: Gretchen D
authors.initials: GD
authors.lastname: Frantz
authors.forename: Nancy
authors.initials: N
authors.lastname: Chiang
authors.forename: Richard
authors.initials: R
authors.lastname: Vandlen
authors.forename: Lauri J
authors.initials: LJ
authors.lastname: Diehl
authors.forename: Nicholas
authors.initials: N
authors.lastname: Skelton
authors.forename: Hok Seon
authors.initials: HS
authors.lastname: Kim
authors.forename: Dan
authors.initials: D
authors.lastname: Eaton
authors.forename: Kerstin N
authors.initials: KN
authors.lastname: Schmidt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D018925:Chemokines / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D019743:Chemokines, CXC; D003713:Dendritic Cells / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009000:Monocytes / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D009928:Organ Specificity; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: C508524:CXCL17 protein, human; D018925:Chemokines; D019743:Chemokines, CXC