title: Analysis of ginkgolides and bilobalide in food products using LC-APCI-MS.
abstract: A method was developed for the extraction and quantification of five marker compounds characteristic of Ginkgo biloba. Five ginkgo terpene trilactones: bilobalide and ginkgolides A, B, C, and J, were selected as marker compounds for this study. Initial studies produced a simple methanol extraction method for determination of gingko markers in solid dietary supplements. Five dietary supplements were analyzed and the results were later compared to the concentrations detected in the analysis of beverages. Beverage samples were prepared by extracting the ginkgo terpene trilactones using an optimized solid phase extraction (SPE) method. The extracts were analyzed using LC-atmospheric pressure chemical ionization (APCI)-MS in the negative ionization mode. The limits of detection of the extraction method ranged from 6.8 to 3.2 ng mL(-1). Using the optimized method, 14 drinks and 3 tea products were analyzed. Concentrations of total marker compounds in drinks ranged between 1685 and 21.4 ng mL(-1) with individual ginkgo terpene trilactones being detected at ppb concentrations. Analysis of brewed tea products presented much higher total marker compound concentrations ranging from 8.12 and 16.6 microg mL(-1). Analytical results reproducibility data, and recovery of the SPE method are presented.
authors.affiliation: U.S. Food and Drug Administration, Centre for Food Safety and Applied Nutrition, 5100 Paint Branch Parkway, College Park, MD, USA. ldejager@cfsan.fda.gov
authors.forename: Lowri S
authors.initials: LS
authors.lastname: de Jager
authors.forename: Gracia A
authors.initials: GA
authors.lastname: Perfetti
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Diachenko
mesh_terms: D001628:Beverages / Q000032:analysis; D002853:Chromatography, Liquid; D003517:Cyclopentanes / Q000032:analysis*; D019587:Dietary Supplements / Q000032:analysis; D004224:Diterpenes / Q000032:analysis*; D005504:Food Analysis; D005663:Furans / Q000032:analysis*; D020441:Ginkgo biloba; D046934:Ginkgolides / Q000032:analysis*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D012015:Reference Standards; D015203:Reproducibility of Results; D013662:Tea / Q000737:chemistry
chemical_list: D003517:Cyclopentanes; D004224:Diterpenes; D005663:Furans; D046934:Ginkgolides; D007202:Indicators and Reagents; D013662:Tea; C073710:bilobalide