Cutting edge: novel human dendritic cell- and monocyte-attracting chemokine-like protein identified by fold recognition methods.
Chemokines play an important role in the immune system by regulating cell trafficking in homeostasis and inflammation. In this study, we report the identification and characterization of a novel cytokine-like protein, DMC (dendritic cell and monocyte chemokine-like protein), which attracts dendritic cells and monocytes. The key to the identification of this putative new chemokine was the application of threading techniques to its uncharacterized sequence. Based on our studies, DMC is predicted to have an IL-8-like chemokine fold and to be structurally and functionally related to CXCL8 and CXCL14. Consistent with our predictions, DMC induces migration of monocytes and immature dendritic cells. Expression studies show that DMC is constitutively expressed in lung, suggesting a potential role for DMC in recruiting monocytes and dendritic cells from blood into lung parenchyma.
Pisabarro
M Teresa
MT
Department of Protein Engineering, Genentech Inc., South Francisco, CA 94080, USA. mayte@biotec.tu-dresden.de
Leung
Beatrice
B
Kwong
Mandy
M
Corpuz
Racquel
R
Frantz
Gretchen D
GD
Chiang
Nancy
N
Vandlen
Richard
R
Diehl
Lauri J
LJ
Skelton
Nicholas
N
Kim
Hok Seon
HS
Eaton
Dan
D
Schmidt
Kerstin N
KN
D000595:Amino Acid Sequence; D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D018925:Chemokines / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D019743:Chemokines, CXC; D003713:Dendritic Cells / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009000:Monocytes / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D009928:Organ Specificity; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
C508524:CXCL17 protein, human; D018925:Chemokines; D019743:Chemokines, CXC