Simultaneous determination of MHD and DMD in dog plasma by high-performance liquid chromatography with fluorescence detection and its application to pharmacokinetic studies.
A rapid, reproducible high-performance liquid chromatographic (HPLC) method with fluorescence detection for the simultaneous determination of 3(or 8)-(1-methoxyethyl)-8(or 3)-(1-hydroxyethyl)-deuteroporphyrin IX (MHD) and 3,8-di-(1-methoxyethyl)-deuteroporphyrin IX (DMD) in dog plasma was described. Fluorescein was used as an internal standard. A simple extraction step with ethyl acetate was performed before chromatography on a Diamonsil C18 column (5 microm, 4.6 mm x 150 mm). The chromatography used 0.02 mol L(-1) sodium acetate/tetrahydrofuran (66:34 v/v). The analytical curve was linear over the concentration range 0.025-2.5 microg mL(-1). For a 100 microL dog plasma sample, the limit of determination for both MHD and DMD was 0.025 microg mL(-1). The recoveries of MHD and DMD were more than 76% and 89%, respectively. The intra-assay (within-run) and interassay (between-run) coefficients of variation (precisions) for MHD and DMD were less than 15%. This method was found to be suitable for the analysis of biosamples and was successfully applied to pharmacokinetic studies of Deuxemether in dogs.
Li
Xiaoyu
X
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, 24# Tong Jia Xiang Street, 210, Nanjing, 210009, People's Republic of China.
Wang
Guangji
G
Xie
Haitang
H
Wang
Rui
R
Xu
Meijuan
M
Wang
Wei
W
Tao
Jining
J
Sun
Jianguo
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D005260:Female; D008297:Male; D017319:Photosensitizing Agents / Q000097:blood* / Q000493:pharmacokinetics; D011166:Porphyrins / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*
D017319:Photosensitizing Agents; D011166:Porphyrins