The structural proteome of Pseudomonas aeruginosa bacteriophage phiKMV.
The structural proteome of phiKMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in phiKMV and suggest subtle structural differences from phage T7.
Lavigne
Rob
R
Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
Noben
Jean-Paul
JP
Biomedical Research Institute, Limburgs Universitair Centrum and School of Life Sciences, University Hasselt, Diepenbeek, B-3590, Belgium.
Hertveldt
Kirsten
K
Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
Ceyssens
Pieter-Jan
PJ
Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
Briers
Yves
Y
Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
Dumont
Debora
D
Biomedical Research Institute, Limburgs Universitair Centrum and School of Life Sciences, University Hasselt, Diepenbeek, B-3590, Belgium.
Roucourt
Bart
B
Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
Krylov
Victor N
VN
State Institute for Genetics and Selection of Industrial Micro-organisms, 1st Dorozhnii proezd 1, Moscow, 113545 Russia.
Mesyanzhinov
Vadim V
VV
Howard Hughes Medical Institute; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya Street 16/10, Moscow, 117991 Russia.|Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
Robben
Johan
J
Biomedical Research Institute, Limburgs Universitair Centrum and School of Life Sciences, University Hasselt, Diepenbeek, B-3590, Belgium.
Volckaert
Guido
G
Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
D017123:Bacteriophage T7 / Q000235:genetics; D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D016679:Genome, Viral; D013058:Mass Spectrometry; D020543:Proteome; D011550:Pseudomonas aeruginosa / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D020543:Proteome; D014764:Viral Proteins