title: Overexpression of NK2 inhibits liver regeneration after partial hepatectomy in mice.
abstract: OBJECTIVE
  To investigate the in vivo effects of NK2 on liver regeneration after partial hepatectomy (PH).
  
  
  METHODS
  Survival after PH was observed with 21 NK2 transgenic mice and 23 wild-type (WT) mice over 10 d. Liver regeneration was analyzed using histology and immunohistochemistry. Expressions of genes were analyzed using Northern blot analysis, immunoprecipitation and immunoblotting, and reverse transcriptase polymerase chain reaction assay. Kaplan-Meier method and the log-rank test were used for analyzing the survival after PH. Differences in the results of immunohistochemistry and percentage of liver regeneration was determined by the Student's t-test.
  
  
  RESULTS
  More than half of NK2 transgenic mice died within 48 h after PH. After PH, increased deposition of small lipid droplets in hepatocytes was evident and hepatic proliferation was inhibited in NK2 transgenic mice. The hepatic expression and kinase activity of HGF receptor, c-Met, were unchanged among WT mice and NK2 transgenic mice after PH. The expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) in liver tissues were prolonged in NK2 transgenic mice that died after PH.
  
  
  CONCLUSIONS
  Our findings indicate that over-expression of NK2 inhibits liver regeneration after PH.
authors.affiliation: Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Otsuka
authors.forename: Norio
authors.initials: N
authors.lastname: Horiguchi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kanda
authors.forename: Takashi
authors.initials: T
authors.lastname: Kosone
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Yuasa
authors.forename: Naondo
authors.initials: N
authors.lastname: Sohara
authors.forename: Satoru
authors.initials: S
authors.lastname: Kakizaki
authors.forename: Ken
authors.initials: K
authors.lastname: Sato
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Takagi
authors.forename: Glenn
authors.initials: G
authors.lastname: Merlino
authors.forename: Masatomo
authors.initials: M
authors.lastname: Mori
mesh_terms: D000818:Animals; D002455:Cell Division / Q000502:physiology; D005260:Female; D015870:Gene Expression; D006498:Hepatectomy / Q000379:methods*; D017228:Hepatocyte Growth Factor / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000502:physiology* / Q000601:surgery; D008115:Liver Regeneration / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D017434:Protein Structure, Tertiary
chemical_list: D017228:Hepatocyte Growth Factor