Utility of the oestrogen-dependent vaginal candidosis murine model in evaluating the efficacy of various therapies against vaginal Candida albicans infection.
The efficacy of yogurt treatment against vaginal candidosis (VC) was examined using an oestrogen-dependent vaginal candidosis (EDVC) murine model. The EDVC mouse model was constructed by inoculating mice with viable Candida albicans cells under pseudo-oestrus conditions. Vaginal fungal burden in the various mouse groups was evaluated at several time points following the induction of VC. Untreated and yogurt-treated naïve mice exhibited background levels of VC (<6000 CFU per mouse). Candida albicans colonisation in untreated EDVC mice was significantly higher (P < 0.05) than that in yogurt-treated EDVC mice at days 20-30. Metronidazole-treated naïve mice developed persistent C. albicans vaginal colonisation at significantly lower levels (P < 0.05) than that in untreated or metronidazole-treated EDVC mice. Lactobacillus was only detected in the reproductive tracts of yogurt-treated naïve and EDVC mice. These findings suggest that the presence of Lactobacillus in the reproductive tract can suppress C. albicans growth and the antibiotics may predispose to VC.
Hamad
Mawieh
M
Department of Biological Sciences, Hashemite University, Zarqa, Jordan. taqiwmohanad@yahoo.com
Muta'eb
Enas
E
Abu-Shaqra
Qasem
Q
Fraij
Abeer
A
Abu-Elteen
Khaled
K
Yasin
Salem R
SR
D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage; D002176:Candida albicans / Q000254:growth & development / Q000302:isolation & purification; D002181:Candidiasis, Vulvovaginal / Q000188:drug therapy / Q000628:therapy*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000254:growth & development / Q000302:isolation & purification; D008795:Metronidazole / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D014621:Vagina / Q000382:microbiology; D015014:Yogurt
D000890:Anti-Infective Agents; D008795:Metronidazole