title: Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins.
abstract: A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.
authors.affiliation: Unidade de Retrovírus e Infecções Associadas, Centro de Patogénese Molecular, Faculdade de Farmácia de Lisboa, Avenida das Forças Armadas, 1649-019 Lisboa, Portugal.
authors.forename: José Maria
authors.initials: JM
authors.lastname: Marcelino
authors.forename: Helena
authors.initials: H
authors.lastname: Barroso
authors.forename: Fátima
authors.initials: F
authors.lastname: Gonçalves
authors.forename: Sofia Marques
authors.initials: SM
authors.lastname: Silva
authors.forename: Carlos
authors.initials: C
authors.lastname: Novo
authors.forename: Perpétua
authors.initials: P
authors.lastname: Gomes
authors.forename: Ricardo
authors.initials: R
authors.lastname: Camacho
authors.forename: Nuno
authors.initials: N
authors.lastname: Taveira
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood*; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D011498:Protein Precursors / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D054299:env Gene Products, Human Immunodeficiency Virus
chemical_list: D015686:Gene Products, env; D015483:HIV Antibodies; D015488:HIV Antigens; D011498:Protein Precursors; D011994:Recombinant Proteins; D054299:env Gene Products, Human Immunodeficiency Virus; C059763:env protein gp36, Human immunodeficiency virus; C066316:gp125 envelope protein, Human immunodeficiency virus 2