title: Ultrasound exposure can increase the membrane permeability of human neutrophil granulocytes containing microbubbles without causing complete cell destruction.
abstract: Activated polymorphonuclear neutrophil (PMN) granulocytes can bind and subsequently phagocytose microbubbles used as ultrasound (US) contrast agents. The purpose of the present study was to assess insonation effects on cell membrane integrity and metabolic activity of activated PMN. Furthermore, we investigated whether or not there is an acoustic threshold at which insonation of PMN results in increase of membrane permeability without causing complete cell destruction. PMN isolated from healthy volunteers were activated with phorbol myristate acetate (PMA) for 15 min to allow phagocytosis of albumin and lipid microbubbles and were subsequently exposed to US with a mechanical index between 0.15 and 1.8. Apoptosis, loss of membrane integrity and formation of cell fragments were evaluated by measurement of lactate dehydrogenase leakage and by double staining with annexin V and propidium iodide, using flow cytometry. Neutrophil superoxide anion generation was measured photometrically. Insonation of activated PMN in the presence of microbubbles amplified apoptosis and lactate dehydrogenase leakage and induced loss of membrane integrity and complete cell destruction with increasing acoustic pressures. The bioeffects observed by insonation with high mechanical indices (1.0 to 1.8), and particularly the formation of cell fragments, were significantly more pronounced in the presence of albumin microbubbles. Insonation in the presence of lipid microbubbles increased cell membrane permeability, but caused significantly less cell destruction and left the metabolic activity of activated PMN uninfluenced. Thus, both albumin and lipid microbubbles induce apoptosis and membrane injury during insonation of activated PMN. However, insonation in the presence of lipid microbubbles seems to influence cell viability to a smaller extent. This could be of advantage in the setting of US-guided local drug delivery. In this setting, increase of membrane permeability may allow bioactive substances to enter into cells, which survive the US treatment, and specifically modify their function.
authors.affiliation: Department of Cardiology, University of Heidelberg, Heidelberg, Germany. Grigorios_Korosoglou@med.uni-heidelberg.de
authors.forename: Grigorios
authors.initials: G
authors.lastname: Korosoglou
authors.forename: Stefan E
authors.initials: SE
authors.lastname: Hardt
authors.forename: Raffi
authors.initials: R
authors.lastname: Bekeredjian
authors.forename: Juergen
authors.initials: J
authors.lastname: Jenne
authors.forename: Mathias
authors.initials: M
authors.lastname: Konstantin
authors.forename: Marco
authors.initials: M
authors.lastname: Hagenmueller
authors.forename: Hugo A
authors.initials: HA
authors.lastname: Katus
authors.forename: Helmut
authors.initials: H
authors.lastname: Kuecherer
mesh_terms: D000418:Albumins / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology; D002463:Cell Membrane Permeability / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D050356:Lipid Metabolism; D045423:Microbubbles; D009504:Neutrophils / Q000378:metabolism / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology; D011312:Pressure; D014465:Ultrasonics
chemical_list: D000418:Albumins; D007770:L-Lactate Dehydrogenase