title: [Changes in tumor necrosis factor-alpha mRNA expression in peripheral polymorphonuclear leukocyte and tissues during posttraumatic sepsis].
abstract: OBJECTIVE
  To evaluate the changes in tumor necrosis factor-alpha (TNF-alpha) mRNA expression in peripheral polymorphonuclear leukocyte (PMNs) and tissues after cecal ligation puncture (CLP) in rats.
  
  
  METHODS
  Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect TNF-alpha mRNA expression in peripheral PMNs and tissues.
  
  
  RESULTS
  The TNF-alpha mRNA expression in peripheral PMNs rose gradually after CLP, and it began to decrease after reaching the peak at 48 hours, but it was still higher than normal. The elevation of TNF-alpha mRNA expression was first limited in the regional tissues (P<0.01 at 12 hours after CLP and peaked at 24 hours in the intestine), then it entered the blood circulation later to affect the sensitive organs--lungs and livers (P<0.01 at 24 hours after CLP and peaked at 48 hours in the lung; P<0.05 at 24 hours after CLP and peaked at 48 hours in the liver).
  
  
  CONCLUSIONS
  The rise of TNF-alpha mRNA expression plays an important role in pathogenesis of sepsis.
authors.affiliation: Department of Orthopaedics, Second Affiliated Hospital of General Hospital of PLA, Beijing 100091, China. jianchengxi309@yahoo.com.cn
authors.forename: Jian-cheng
authors.initials: JC
authors.lastname: Xi
authors.forename: Yuan-zheng
authors.initials: YZ
authors.lastname: Ma
authors.forename: Bao-tong
authors.initials: BT
authors.lastname: Zhou
authors.forename: Hong-yuan
authors.initials: HY
authors.lastname: Lin
authors.forename: Zhong-jie
authors.initials: ZJ
authors.lastname: He
authors.forename: Xing-huo
authors.initials: XH
authors.lastname: Zhang
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Fu
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Sheng
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000209:etiology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha