title: Effects of Helicobacter pylori infection on gastric epithelial cell kinetics in patients with chronic renal failure.
abstract: OBJECTIVE
  To evaluate the effects of Helicobacter pylori infection on gastric epithelial cell kinetics in patients with chronic renal failure (CRF).
  
  
  METHODS
  Forty-four patients were enrolled in this study and divided into four groups with respect to their Helicobacter pylori (H pylori) and CRF status. Groups were labeled as follows: 1a: normal renal function, H pylori negative (n = 12), 1b: normal renal function, H pylori positive (n = 11), 2a: CRF, H pylori negative (n = 10), 2b: CRF, H pylori positive (n = 11). Upper gastrointestinal endoscopy was done in all the patients involved in the study. During endoscopical investigation, antral biopsy specimens were taken from each patient. In order to evaluate the cell apoptosis and proliferation in gastric epithelial cells, Bax and proliferating cell nuclear antigen (PCNA) labeling indexes (LI) were assessed with immunohistochemical staining method.
  
  
  RESULTS
  For groups 1a, 1b, 2a, and 2b, mean Bax LI was identified as 34.4+/-13.7, 44.1+/-16.5, 46.3+/-20.5, 60.7+/-13.8, respectively and mean PCNA LI was identified as 36.2+/-17.2, 53.6+/-25.6, 59.5+/-25.6, 67.2+/-22, respectively. When the one-way ANOVA test was applied, statistically significant differences were detected between the groups for both Bax LI (P = 0.004 <0.01) and PCNA LI (P = 0.009 <0.01). When groups were compared further in terms of Bax LI and PCNA LI with Tukeyos HSD test for multiple pairwise comparisons, statistically significant difference was observed only between groups 1a and 2b (P = 0.006 <0.01).
  
  
  CONCLUSIONS
  In gastric epithelial cells, expression of both the pre-apoptotic protein Bax and the proliferation marker PCNA increase with H pylori infection. This increase is more evident in patients with uremia. These findings suggest that uremia accelerates apoptosis and proliferation in gastric epithelial cells.
authors.affiliation: Department of Gastroenterology, Zonguldak Karaelmas University Faculty of Medicine, Zonguldak 67800, Turkey. selimaydemir@hotmail.com
authors.forename: Selim
authors.initials: S
authors.lastname: Aydemir
authors.forename: Binnaz Handan
authors.initials: BH
authors.lastname: Ozdemir
authors.forename: Gurden
authors.initials: G
authors.lastname: Gur
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Dogan
authors.forename: Ugur
authors.initials: U
authors.lastname: Yilmaz
authors.forename: Sedat
authors.initials: S
authors.lastname: Boyacioglu
mesh_terms: D000328:Adult; D017209:Apoptosis; D016022:Case-Control Studies; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology*; D016481:Helicobacter Infections / Q000150:complications* / Q000473:pathology*; D016480:Helicobacter pylori; D006801:Humans; D007676:Kidney Failure, Chronic / Q000150:complications*; D007700:Kinetics; D008297:Male; D008875:Middle Aged