title: Synthesis and application of negatively charged PNA analogues.
abstract: Negatively charged DNA mimics containing phosphonate analogoues of peptide nucleic acids were designed, and their physicochemical and biological properties were evaluated in the comparison with natural oligonucleotides, classical peptide nucleic acids, and morpholino phosphorodiamidate oligonucleotide analogues. The results obtained revealed a high potential of phosphonate-containing PNA derivatives for a number of biological applications, such as diagnostic, nucleic acids analysis, and inhibition of gene expression.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. eva@mail.ibch.ru
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Efimov
authors.forename: Oksana G
authors.initials: OG
authors.lastname: Chakhmakhcheva
authors.forename: Eric
authors.initials: E
authors.lastname: Wickstrom
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics; D018390:Gene Targeting / Q000379:methods; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000235:genetics / Q000494:pharmacology; D034622:RNA Interference / Q000187:drug effects; D015027:Zebrafish / Q000196:embryology / Q000235:genetics
chemical_list: D009841:Oligonucleotides; D020135:Peptide Nucleic Acids