title: Endothelin-3 production by human rhabdomyosarcoma: a possible new marker with a paracrine role.
abstract: Several autocrine and paracrine growth factor circuits have been found in human rhabdomyosarcoma cells. In this study we show that endothelin-3 (ET-3), a vasoactive peptide, is produced by human rhabdomyosarcoma cell lines, whereas it is not expressed by human sarcoma cell lines of non-muscle origin. We did not find evidence of a significant autocrine loop; nevertheless ET-3 produced by rhabdomyosarcoma cells can act as a paracrine factor, since it promotes migration of endothelial cells. Moreover ET-3 is present in plasma of mice bearing xenografts of human rhabdomyosarcoma cells, and may be potential new marker of the human rhabdomyosarcoma to be studied further.
authors.affiliation: Cancer Research Section, Department of Experimental Pathology, University of Bologna, Viale Filopanti 22, I-40126 Bologna, Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Palladini
authors.forename: Annalisa
authors.initials: A
authors.lastname: Astolfi
authors.forename: Stefania
authors.initials: S
authors.lastname: Croci
authors.forename: Carla
authors.initials: C
authors.lastname: De Giovanni
authors.forename: Giordano
authors.initials: G
authors.lastname: Nicoletti
authors.forename: Angelo
authors.initials: A
authors.lastname: Rosolen
authors.forename: Francesca
authors.initials: F
authors.lastname: Sartori
authors.forename: Pier-Luigi
authors.initials: PL
authors.lastname: Lollini
authors.forename: Lorena
authors.initials: L
authors.lastname: Landuzzi
authors.forename: Patrizia
authors.initials: P
authors.lastname: Nanni
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D019334:Endothelin-3 / Q000096:biosynthesis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D009842:Oligopeptides / Q000494:pharmacology; D012516:Osteosarcoma / Q000378:metabolism; D019899:Paracrine Communication; D010880:Piperidines / Q000494:pharmacology; D017466:Receptors, Endothelin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012208:Rhabdomyosarcoma / Q000378:metabolism*; D012512:Sarcoma, Ewing / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D019334:Endothelin-3; D009842:Oligopeptides; D010880:Piperidines; D017466:Receptors, Endothelin; C086539:BQ 788