title: Fluoroquinolone resistance linked to both gyrA and parC mutations in the quinolone resistance-determining region of Shigella dysenteriae type 1.
abstract: We examined the quinolone resistance-determining region (QRDR) of gyrA, gyrB, and parC of recently isolated fluoroquinolone-resistant S. dysenteriae type 1 strains from south Asia and compared data with fluoroquinolone-susceptible strains associated with previous epidemics of 1978, 1984, and 1994. In fluoroquinolone-resistant strains, double mutations (Ser83-->Leu, Asp87-->Asn or Gly) and a single mutation (Ser80-->Ile) were detected in the QRDRs of gyrA and parC, respectively.
authors.affiliation: ICDDR, B: Centre for Health and Population Research, GPO Box-128, Dhaka, 1000, Bangladesh. kaisar@icddrb.org
authors.forename: Kaisar A
authors.initials: KA
authors.lastname: Talukder
authors.forename: Bijay K
authors.initials: BK
authors.lastname: Khajanchi
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Islam
authors.forename: Zhahirul
authors.initials: Z
authors.lastname: Islam
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Dutta
authors.forename: Mustafizur
authors.initials: M
authors.lastname: Rahman
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
authors.forename: Gopinath B
authors.initials: GB
authors.lastname: Nair
authors.forename: David A
authors.initials: DA
authors.lastname: Sack
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001210:Asia, Southeastern; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004405:Dysentery, Bacillary / Q000382:microbiology; D024841:Fluoroquinolones / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D015363:Quinolones / Q000494:pharmacology*; D012762:Shigella dysenteriae / Q000187:drug effects* / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D015363:Quinolones; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase