Detection of Tuber melanosporum DNA in soil.
Our objectives were (i) to develop a molecular method to detect mycelia of Tuber melanosporum (black truffle) in soil and (ii) to test for mycelial distribution around two truffle-bearing Quercus ilex trees in a truffle orchard. Isolation of total DNA from soil was performed, followed by PCR amplification with T. melanosporum-specific primers and restriction analysis. To address the detection sensitivity level, soil samples were inoculated with known amounts of gleba of T. melanosporum. The detection limit was >/=11.4 mug of hyphae g(-1) of soil. Mycelium was detected primarily within the area defined by the truffle burn and within the top 35 cm of the soil in all directions from the trees.
Suz
Laura M
LM
Centre Tecnològic Forestal de Catalunya, Solsona, Spain. laura.martinez@ctfc.es
Martín
María P
MP
Colinas
Carlos
C
D001203:Ascomycota / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D029963:Quercus; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D017931:DNA Primers; D004271:DNA, Fungal; D012987:Soil