title: [Autologus bone marrow-derived mesenchymal stem cells intracoronary delivery after acute myocardial infarction in miniature pig].
abstract: OBJECTIVE
  To evaluate the possibility that using intracoronary delivery of autologus bone marrow-derived mesenchymal stem cells (MSCs) to improve the cardiac function after acute myocardial infarction (AMI) in miniature pig.
  
  
  METHODS
  MSCs were cultured in Dulbecco's modified Eagle's medium-F12 (DMEM/F12) medium. AMI model was made by blocking the blood stream of the first diagonal branch in miniature pig, and released the branch after 90 minutes. After 10-14 days, (4-6) x 10(7) culture-expanded autologus 4', 6-diamidino-2-phenylindole (DAPI)-labelled MSCs were transplanted into each host heart's AMI area through intracoronary way. Ultrasonic cardiography (UCG) was performed to observe the left ventricular function at 3 months after transplantation. The cellular transplanted hearts were harvested and investigated by immunohistochemical analysis.
  
  
  RESULTS
  Left ventricular function of the MSCs group was improved significantly 3 months later compared with the control group [(54.65 +/- 3.39) vs (43.98 +/- 4.21)%, (P < 0.01)]. Exogenous MSCs survived and site-differentiated into cardiomyocytes in infracted hearts.
  
  
  CONCLUSIONS
  MSCs can play a benificial role to repair damaged heart. Heart function can be improved after MSCs transplantation in porcine myocardial infarction model.
authors.affiliation: Department of Coronary Heart Disease, Fuwai Hospital, CAMS and PUMC, Beijing 100037, China.
authors.forename: Cheng-qian
authors.initials: CQ
authors.lastname: Yin
authors.forename: Ji-lin
authors.initials: JL
authors.lastname: Chen
authors.forename: Yun-fang
authors.initials: YF
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Cao
authors.forename: Rui
authors.initials: R
authors.lastname: Cheng
authors.forename: Xue-tao
authors.initials: XT
authors.lastname: Pei
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D013552:Swine; D013556:Swine, Miniature; D014182:Transplantation, Autologous; D016896:Treatment Outcome