A two-center international evaluation of the Immulite 2000 automated serum gastrin assay.
OBJECTIVE
To assess the performance of the Immulite 2000 serum gastrin assay.


METHODS
Two-center validation and comparison with two manual gastrin assays.


RESULTS
Serum, serum separator, EDTA and heparin tube results differed <20%. Samples showed <20% degradation for <6 h at room temperature, 3-day-refrigerated, and underwent 3 freeze-thaw cycles. Imprecision was <8% between 20 and 824 ng/L (10-393 pmol/L). Spike recovery was 88.5-106%, and recovery on dilution was 62-135%. There were no interferences from gastrin 1-14, pentagastrin, and cholecystokinin (CCK) 1-33 and CCK 26-33. The fasting reference range was <100 ng/L (<48 pmol/L). Regression slopes to the manual assays were 0.9 and 0.97, with comparable clinical performance.


CONCLUSIONS
The Immulite 2000 assay is precise, accurate, and fast and compares well with established gastrin assays.
Eastvold
Mary L
ML
Endocrine Laboratory, Department of Laboratory Medicine and Pathology, Hilton 730C, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USA.
Wallinder
Hans W
HW
Preissner
Carol M
CM
Singh
Ravinder J
RJ
Löwbeer
Christian
C
Grebe
Stefan K G
SK
D001331:Automation; D003429:Cross Reactions; D005755:Gastrins / Q000097:blood*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results
D005755:Gastrins