Metal resistance in Candida biofilms.
Yeasts are often successful in metal-polluted environments; therefore, the ability of biofilm and planktonic cell Candida tropicalis to endure metal toxicity was investigated. Fifteen water-soluble metal ions, chosen to represent groups 6A to 6B of the periodic table, were tested against this organism. With in vitro exposures as long as 24 h, biofilms were up to 65 times more tolerant to killing by metals than corresponding planktonic cultures. Of the most toxic heavy metals tested, only very high concentrations of Hg2+, CrO4 (2-) or Cu2+ killed surface-adherent Candida. Metal-chelator precipitates could be formed in biofilms following exposure to the heavy metals Cu2+ and Ni2+. This suggests that Candida biofilms may adsorb metal cations from their surroundings and that sequestration in the extracellular matrix may contribute to resistance. We concluded that biofilm formation may be a strategy for metal resistance and/or tolerance in yeasts.
Harrison
Joe J
JJ
Department of Biological Sciences, University of Calgary, Calgary, Canada.
Rabiei
Maryam
M
Turner
Raymond J
RJ
Badry
Erin A
EA
Sproule
Kimberley M
KM
Ceri
Howard
H
D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D041022:Candida tropicalis / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media; D025141:Drug Resistance, Fungal; D019216:Metals, Heavy / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D010933:Plankton / Q000187:drug effects / Q000254:growth & development
D003470:Culture Media; D019216:Metals, Heavy