Validation of the measurement of low concentrations of 5-hydroxytryptamine in plasma using high performance liquid chromatography.
A sensitive and rapid assay is described for the measurement of low concentrations of 5-hydroxytryptamine (5-HT) present in human platelet-depleted plasma (PDP) using reverse-phase high performance liquid chromatography (HPLC) with fluorimetric detection. With an analysis time of 12 min, this method is particularly useful for large-scale clinical trials investigating small differences in PDP 5-HT concentrations in conditions such as functional gastrointestinal disorders (FGID). The limit of detection and quantification were 1 and 3 nmol/l, respectively, and the calibration curve linear between 1 and 1000 nmol/l. The within-day and between-day precision were 4.3 and <13.6%, respectively.
Atkinson
Wendy
W
Department of Medicine, South Manchester University Hospitals NHS Trust, UK.
Lockhart
Stephen J
SJ
Houghton
Lesley A
LA
Keevil
Brian G
BG
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000097:blood*; D013050:Spectrometry, Fluorescence
D012701:Serotonin