[Genome-wide analysis for transcriptional regulation of chondrogenesis-related genes].
Transcriptional factor Sox9 plays a central role in development of chondrogenesis with stage specificity and in tissue specificity. Sox9 with a number of transcriptional cofactors is a critical regulator in genes such as COL2, chondorocyte specific gene. We report here our new large scale approaches : 1) Whole mount in situ hybridization (WISH), 2) High through-put human cDNA transfection assay. These genome-wide approaches have a possibility of discovery for new transcription factors in chondrogenesis.
Asahara
Hiroshi
H
Department of Regenarative Biology and Medicine, National Research Institute for Child Health and Development.
Asada
Maki
M
D000818:Animals; D020219:Chondrogenesis / Q000235:genetics*; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D055758:SOX9 Transcription Factor; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
D006609:High Mobility Group Proteins; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D014157:Transcription Factors