Protective effect of selected flavonoids on in vitro daunorubicin-induced cardiotoxicity.
Flavonoids are an ubiquitous group of polyphenolic substances with varied chemical structures present in foods of plant origin and act as free radical scavenging and chelating agents with a variety of biological activities. Using a model of spontaneously beating, cultured adult rat cardiomyocytes, this study examined the cardioprotective role of quercetin, naringenin, pycnogenol and a model antioxidant, trolox, against daunorubicin-induced toxicity. Cardiomyocyte protection was assessed by MTT test and extracellular lactate dehydrogenase detection. Protection of cardiomyocytes was concentration/dose dependent for quercetin > naringenin > pycnogenol > trolox. Quercetin (10(-4)-10(-6) mol/L) after 24 h of co-incubation with daunorubicin significantly increased the cardiomyocyte survival in all tested concentrations (p < 0.001). The cytoprotective effect of naringenin (10(-4)-10(-6) mol/L) was similar to those of quercetin (p < 0.001 and p < 0.01, respectively). Pycnogenol was the least effective of the flavonoids studied. On the other hand, all tested flavonoids had significantly better protective effects than trolox. The leakage of lactate dehydrogenase induced by daunorubicin was also prevented by the studied compounds and was in accordance with their cytoprotective activity.
Mojzisová
G
G
Department of Experimental Medicine, Medical Faculty, University of P.J. Safarik Kosice, Slovak Republic. mojzis@central.medic.upjs.sk
Mirossay
L
L
Kucerová
D
D
Kyselovic
J
J
Mirossay
A
A
Mojzis
J
J
D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D000975:Antioxidants / Q000494:pharmacology; D009202:Cardiomyopathies / Q000139:chemically induced / Q000517:prevention & control*; D003630:Daunorubicin / Q000009:adverse effects*; D005419:Flavonoids / Q000009:adverse effects / Q000494:pharmacology*; D006325:Heart Atria / Q000166:cytology; D066298:In Vitro Techniques; D032383:Myocytes, Cardiac / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000970:Antineoplastic Agents; D000975:Antioxidants; D005419:Flavonoids; D011794:Quercetin; D003630:Daunorubicin