Cloning and characterization of a gene encoding a cold-shock protein in Lactobacillus casei.
One csp-like gene, called cspA, from the lactic acid bacterium Lactobacillus casei was identified by an inverse polymerase chain reaction approach based on degenerate primers. cspA encodes a protein of 66 amino acid residues, which displays at least 74% identity with Csp proteins of Lactobacillus genera. Northern blot analysis revealed that cspA is transcribed monocistronically and that its expression is induced after a temperature downshift from 37 degrees C to 20 degrees C. The transcriptional start site has been determined and is situated 98 bp upstream of the initiation codon. A cspA mutant strain was constructed and it showed reduced growth rate compared with the wild type at both optimal and low temperatures, demonstrating that CspA plays an important role in the physiology of L. casei.
Sauvageot
Nicolas
N
Laboratoire de Microbiologie de l'Environnement, Université de Caen, Esplanade de la Paix, Caen Cedex, France.
Beaufils
Sophie
S
Mazé
Alain
A
Deutscher
Josef
J
Hartke
Axel
A
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D005615:Freezing; D015964:Gene Expression Regulation, Bacterial; D018869:Heat-Shock Response; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; C062260:cold shock protein CS7.4, Bacteria