Regulation of G protein-coupled receptor trafficking by inefficient plasma membrane expression: molecular basis of an evolved strategy.
Despite the prevalence of G protein-coupled receptors as transducers of signals from hormones, neurotransmitters, odorants, and light, little is known about mechanisms that regulate their plasma membrane expression (PME), although misfolded receptors are recognized and retained by a cellular quality control system (QCS). Convergent evolution of the gonadotropin-releasing hormone (GnRH) receptor (GnRHR) progressively decreases inositol phosphate production in response to agonist, validated as a measure of PME of receptor. A pharmacological chaperone that optimizes folding also increases PME of human, but not of rat or mouse, GnRHR because a higher percentage of human GnRHRs are misfolded structures due to their failure to form an apparent sulfhydryl bridge, and they are retained by the QCS. Bridge formation is increased by deleting (primate-specific) Lys191. In rat or mouse GnRHR that lacks Lys191, the bridge is non-essential and receptor is efficiently routed to the plasma membrane. Addition of Lys191 alone to the rat sequence did not diminish PME, indicating that other changes are required for its effects. A strategy, based on identification of amino acids that both 1) co-evolved with the Lys191 and 2) were thermodynamically unfavorable substitutions, identified motifs in multiple domains of the human receptor that control the destabilizing influence of Lys191 on a particular Cys bridge, resulting in diminished PME. The data show a novel and underappreciated means of posttranslational control of a G protein-coupled receptor by altering its interaction with the QCS and provide a biochemical explanation of the basis of disease-causing mutations of this receptor.
Janovick
Jo Ann
JA
Division of Neuroscience, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon 97006, USA.
Knollman
Paul E
PE
Brothers
Shaun P
SP
Ayala-Yáñez
Rodrigo
R
Aziz
Abeer S
AS
Conn
P Michael
PM
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002064:Buserelin / Q000494:pharmacology; D019556:COS Cells; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003545:Cysteine / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D007295:Inositol Phosphates / Q000032:analysis / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D017354:Point Mutation; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism*; D011966:Receptors, LHRH / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013816:Thermodynamics
D019086:Bridged Bicyclo Compounds, Heterocyclic; D004220:Disulfides; C461300:IN3 compound; D007211:Indoles; D007295:Inositol Phosphates; D011725:Pyridines; D043562:Receptors, G-Protein-Coupled; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone; D008239:Lysine; D003545:Cysteine; D002064:Buserelin