5alpha-reductase inhibitory effect of triterpenoids isolated from Ganoderma lucidum.
5alpha-Reductase inhibitory activity-guided fractionation of the EtOH extract of the fruiting body of Ganoderma lucidum (LEYSS.:FR.) KARST. (Ganodermataceae), which is called Reishi, or Mannentake in Japan and Lingzhi in China, led to the isolation of two active compounds which were ganoderic acid DM and 5alpha-lanosta-7,9(11),24-triene-15alpha,26-dihydroxy-3-one with an IC(50) of 10.6 microM and 41.9 microM respectively. A carboxyl group of side chain of ganoderic acid DM is essential to elicit the inhibitory activity because of much less activity of its methyl ester.
Liu
Jie
J
Department of Forest and Forest Products Science, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
Kurashiki
Kenji
K
Shimizu
Kuniyoshi
K
Kondo
Ryuichiro
R
D000818:Animals; D042944:Cholestenone 5 alpha-Reductase / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D032902:Ganoderma / Q000737:chemistry*; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D014315:Triterpenes; D042944:Cholestenone 5 alpha-Reductase