Deinococcus mumbaiensis sp. nov., a radiation-resistant pleomorphic bacterium isolated from Mumbai, India.
A radiation-resistant, Gram-negative and pleomorphic bacterium (CON-1) was isolated from a contaminated tryptone glucose yeast extract agar plate in the laboratory. It was red pigmented, nonmotile, nonsporulating, and aerobic, and contained MK-8 as respiratory quinone. The cell wall of this bacterium contained ornithine. The major fatty acids were C16:0, C16:1, C17:0, C18:1 and iso C18:0. The DNA of CON-1 had a G+C content of 70 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that CON-1 exhibited a maximum similarity (94.72%) with Deinococcus grandis. Based on the genotypic, phenotypic and chemotaxonomic characteristics, the bacterium CON-1 was identified as a new species of the genus Deinococcus, for which the name Deinococcus mumbaiensis sp. nov. is proposed. The type strain of D. mumbaiensis is CON-1 (MTCC 7297(T)=DSM 17424(T)).
Shashidhar
Ravindranath
R
Food Technology Division, Bhabha Atomic Research Centre, Mumbai, India.
Bandekar
Jayant R
JR
D000362:Agar; D015373:Bacterial Typing Techniques; D001482:Base Composition; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D034301:Deinococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects*; D004866:Equipment Contamination; D005720:Gamma Rays; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000362:Agar