title: Detection of catheter-related bloodstream infections by the Gram stain-acridine orange leukocyte cytospin test in hematopoietic stem cell transplant recipients.
abstract: In patients with central venous catheters (CVCs), catheter-related bloodstream infections (CRBI) are a prominent cause of morbidity, excess hospital costs, and in some cases mortality. The aim of this prospective study was to assess the validity of the Gram stain-acridine orange leukocyte cytospin (AOLC) test for the diagnosis of CRBI in hematopoietic stem cell transplant (HSCT) recipients with nontunnelled CVCs, using the differential-time-to-positivity (DTP)/clinical criteria as the criterion standard to define CRBIs. CVCs were externalized, nontunnelled, polyurethane double lumen catheters (Arrows, Readings, USA). All CVCs were placed in the subclavian vein by the infraclavicular approach, in the operating room. Catheters were inserted percutaneously, using the Seldinger technique. Study catheters were not exchanged over guidewires. Between May 2002 and December 2004, a total of 245 consecutive patients were included. Twenty-six of the 245 patients (10.6%) had CRBI as determined by the DTP method. The Gram stain-AOLC was positive in only two patients (7.6%) with a CRBI. Our results suggest that the Gram stain-AOLC test is not useful for the diagnosis of catheter-related bloodstream infection in HSCT recipients.2006.
authors.affiliation: Centre National de Greffe de Moelle Osseuse, Tunis, Tunisia. aabdelkefi@yahoo.fr
authors.forename: A
authors.initials: A
authors.lastname: Abdelkefi
authors.forename: W
authors.initials: W
authors.lastname: Achour
authors.forename: L
authors.initials: L
authors.lastname: Torjman
authors.forename: T
authors.initials: T
authors.lastname: Ben Othman
authors.forename: S
authors.initials: S
authors.lastname: Ladeb
authors.forename: A
authors.initials: A
authors.lastname: Lakhal
authors.forename: H
authors.initials: H
authors.lastname: Allouche
authors.forename: A
authors.initials: A
authors.lastname: Ben Hassen
authors.forename: A
authors.initials: A
authors.lastname: Ben Abdeladhim
mesh_terms: D000293:Adolescent; D000328:Adult; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D001424:Bacterial Infections / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology*; D002405:Catheterization, Central Venous / Q000009:adverse effects*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005840:Gentian Violet; D006801:Humans; D008297:Male; D008875:Middle Aged; D010619:Phenazines; D011446:Prospective Studies; D015203:Reproducibility of Results; D033581:Stem Cell Transplantation / Q000009:adverse effects* / Q000379:methods; D014416:Tunisia
chemical_list: C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet