[Chemical coupling of anti-dNA antibody on collagen coating].
OBJECTIVE
To evaluate the feasibility and stability of chemically conjugating IgM on collagen films.


METHODS
IgM was labeled with 125I using the chloramine-T method. Six collagen films were randomly divided into two groups. In chemical coupling group 125I-labeled IgM was chemically coupled with the films through N-succinmiclyl-3- (2-pyridyl-dithio) propionate reaction. In control group 125I-labeled IgM was absorbed onto collagen films. The amount of IgM on the collagen films and the amount of IgM remained on the films after extensive rinsing with phosphate buffered saline were monitored by counting the radioactivity of 125I.


RESULTS
The amount of antibodies loaded onto collagen films in the chemical coupling group was 15 times higher than that on the control films, showing significant statistical difference (P < 0.01). And the stability of conjugation antibodies on collagen films was significantly better than the control films.


CONCLUSIONS
Chemical coupling is an effective approach to immobilize antibodies on collagen for further plasmid DNA tethering.
Liu
Lan-xia
LX
Key Laboratory of Biomaterials, Institute of Biomedical Engineering, CAMS and PUMC, Tianjin 300192, China.
Leng
Xi-gang
XG
Yue
Jing-yin
JY
Song
Li-ping
LP
Wang
Hai
H
Song
Cun-xian
CX
D015906:Angioplasty, Balloon, Coronary / Q000295:instrumentation; D000818:Animals; D000974:Antibodies, Antinuclear / Q000378:metabolism*; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D005822:Genetic Vectors; D007075:Immunoglobulin M / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D011485:Protein Binding; D015607:Stents; D013499:Surface Properties
D000974:Antibodies, Antinuclear; D020099:Coated Materials, Biocompatible; D007075:Immunoglobulin M; D003094:Collagen