Potential for ultrafast protein separations with capillary-channeled polymer (C-CP) fiber columns.
Capillary-channeled polymer (C-CP) fibers are demonstrated as a potential stationary phase for liquid chromatography separation of protein mixtures. Separation of a synthetic mixture of four proteins is accomplished within a 45-second window using a conventional revered-phase (RP) gradient, at a mobile phase flow rate of 7 mL/min (10,200 mm/min).
Nelson
Dwella M
DM
Department of Chemistry, Biosystems Research Complex, Clemson University, Clemson, SC 29634-0973, USA.
Marcus
R Kenneth
RK
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D011108:Polymers; D011506:Proteins