title: Study on the interaction of bovine serum albumin with acid cyanine 5R and its application in analysis.
abstract: A supramolecular complex of bovine serum albumin (BSA) with acid cyanine 5R (AC 5R, C.I. acid blue 113, C.I.: 26360) has been shown to form in Tris-HCl buffer solution (pH 7.42) by linear sweep voltammetry (LSV), fluorimetry, and spectrophotometry. The binding ratio and binding constant of BSA with AC 5R have been detected by LSV and fluorimetry. The binding mechanism is also preliminarily discussed. In Tris-HCl buffer solution (pH 7.42), AC 5R can easily be reduced on the mercury electrode, and it has a well-defined LSV peak current (Ip) and peak potential (Ep) at -0.65 V (vs. SCE). In the presence of BSA, the Ip of AC 5R decreases, and the peak potential (Ep) shifts to a more positive potential. The decrease of the second-order derivative of reductive peak current (deltaIp'') of AC 5R is proportional to the logarithm of BSA concentration in the range of 1.54 x 10(-8) mol x L(-1)-1.54 x 10(-5) mol x L(-1) (r = 0.9931-0.9977). The limit of detection of BSA is 9.0 x 10(-9) mol x L(-1). The relative standard deviation is 1.83% (n = 10), and the standard recovery is 97.5%-104.8%. This method can be used to determine BSA concentration on the basis of the interaction of BSA with AC 5R.
authors.affiliation: Chemistry Department, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Lu
authors.forename: Jing-Hao
authors.initials: JH
authors.lastname: Pan
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Hongfen
authors.initials: H
authors.lastname: Zhang
authors.forename: Yujing
authors.initials: Y
authors.lastname: Guo
authors.forename: Yingte
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001391:Azo Compounds / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D001391:Azo Compounds; D012710:Serum Albumin, Bovine; C015967:Levanol Fast Cyanine 5RN