Influence of CrgA on assembly of the cell division protein FtsZ during development of Streptomyces coelicolor.
The product of the crgA gene of Streptomyces coelicolor represents a novel family of small proteins. A single orthologous gene is located close to the origin of replication of all fully sequenced actinomycete genomes and borders a conserved gene cluster implicated in cell growth and division. In S. coelicolor, CrgA is important for coordinating growth and cell division in sporogenic hyphae. In this study, we demonstrate that CrgA is an integral membrane protein whose peak expression is coordinated with the onset of development of aerial hyphae. The protein localizes to discrete foci away from growing hyphal tips. Upon overexpression, CrgA localizes to apical syncytial cells of aerial hyphae and inhibits the formation of productive cytokinetic rings of the bacterial tubulin homolog FtsZ, leading to proteolytic turnover of this major cell division determinant. In the absence of known prokaryotic cell division inhibitors in actinomycetes, CrgA may have an important conserved function influencing Z-ring formation in these bacteria.
Del Sol
Ricardo
R
Institute of Life Science, School of Medicine, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, United Kingdom.
Mullins
Jonathan G L
JG
Grantcharova
Nina
N
Flärdh
Klas
K
Dyson
Paul
P
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D001483:Base Sequence; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D013171:Spores, Bacterial; D048372:Streptomyces coelicolor / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D001426:Bacterial Proteins; C406769:CrgA protein, Neisseria meningitidis; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D014157:Transcription Factors