The simultaneous separation and determination of chloropropanols in soy sauce and other flavoring with gas chromatography-mass spectrometry in negative chemical and electron impact ionization modes.
Both gas chromatography-mass spectrometry in electron ionization (GC-MS-EI) and negative chemical ionization (GC-MS-NCI) modes are reported in this paper for the simultaneous determination of 1,3-dichloropropan-2-ol (1,3-DCP), 2,3-dichloropropan-1-ol (2,3-DCP), 3-chloropropane-1,2-diol (3-MCPD) and 2-chloropropane-1,3-diol (2-MCPD) in soy sauce and other flavoring. D(5)-3-MCPD (for 3-MCPD and 2-MCPD) and d(5)-1,3-DCP (for 1,3-DCP and 2,3-DCP) were used as the deuterium isotopic labelled internal standards. The feasibility of using heptafluorobutyric anhydride modified with triethylamine (HFBA-Et(3)N) as a new derivatization reagent to replace heptafluorobutyrylimidazole (HFBI) is proposed. Liquid/liquid extraction with hexane was introduced for high lipid content samples. A small survey was carried out of soy sauces (103 samples) and instant noodles (45 samples) and the applicability of GC-MS-NCI and GC-MS-EI was assessed in these different matrices.
Xu
Xiaomin
X
Laboratory of Food Safety and Hygienic Chemistry Analysis, Zhejiang Provincial Center for Disease Prevention and Control, Hangzhou, 310009, People's Republic of China. chemxuxm@163.com
Ren
Yiping
Y
Wu
Pinggu
P
Han
Jianlong
J
Shen
Xianghong
X
D002273:Carcinogens / Q000032:analysis; D002629:Chemosterilants / Q000032:analysis; D002728:Chlorohydrins / Q000032:analysis; D003432:Cross-Linking Reagents / Q000032:analysis; D005021:Ethylamines / Q000737:chemistry; D005421:Flavoring Agents / Q000032:analysis*; D005466:Fluorocarbons / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D045730:Soy Foods / Q000032:analysis*; D000517:alpha-Chlorohydrin / Q000031:analogs & derivatives / Q000032:analysis*
D002273:Carcinogens; D002629:Chemosterilants; D002728:Chlorohydrins; D003432:Cross-Linking Reagents; D005021:Ethylamines; D005421:Flavoring Agents; D005466:Fluorocarbons; C033489:1,3-dichloro-2-propanol; C007924:heptafluorobutyric anhydride; C076159:2,3-dichloro-1-propanol; D000517:alpha-Chlorohydrin; C016162:triethylamine