Effects of a manganese (III) porphyrin catalytic antioxidant in a mouse closed head injury model.
Closed head injury induces cerebral oxidative stress. The efficacy of a Mn (III) porphyrin catalytic antioxidant was assessed in a mouse closed head injury model. Mice were subjected to closed head injury and treated 15 min later with an i.v. bolus of vehicle or 3 mg/kg MnTE-2-PyP5+. Aconitase activity, Fluoro-Jade staining, glial fibrillary acidic protein immunoreactivity, and rotarod falling latencies were measured. Closed head injury altered all variables. MnTE-2-PyP5+ had no effect on any variable with the exception of attenuation of aconitase inactivation at 2 h post-closed head injury. In a second experiment, mice received 3 mg/kg or 6 mg/kg MnTE-2-PyP5+ or vehicle i.v. 15 min post-closed head injury. Rotarod and Morris water maze latencies were measured. Closed head injury altered performance in both tests. No statistically significant effect of MnTE-2-PyP5+ was observed. We conclude that single dose MnTE-2-PyP5+ does not alter outcome in this mouse closed head injury model.
Leinenweber
Stephen B
SB
Department of Pediatrics, Duke University Medical Center, Durham NC 27710, USA.
Sheng
Huaxin
H
Lynch
John R
JR
Wang
Haichen
H
Batinić-Haberle
Ines
I
Laskowitz
Daniel T
DT
Crapo
James D
JD
Pearlstein
Robert D
RD
Warner
David S
DS
D000154:Aconitate Hydratase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002540:Cerebral Cortex / Q000737:chemistry / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D016489:Head Injuries, Closed / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D007150:Immunohistochemistry; D007275:Injections, Intravenous; D008297:Male; D008345:Manganese / Q000737:chemistry; D018782:Maze Learning / Q000187:drug effects; D008665:Metalloporphyrins / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000187:drug effects; D045442:Rotarod Performance Test; D013997:Time Factors
D000975:Antioxidants; D005904:Glial Fibrillary Acidic Protein; D008665:Metalloporphyrins; C431023:manganese (III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin; D008345:Manganese; D000154:Aconitate Hydratase