[Inter-laboratory validation studies of biological method for determination of tetracyclines in royal jelly].
Inter-laboratory validation studies were conducted in 5 laboratories to validate the biological method for determination of tetracyclines in royal jelly. Oxytetracycline spiked at the levels of 0.2 and 1.0 ppm was analyzed. Mean recoveries were 88 and 90%, reproducibility relative standard deviations (RSD(R)) were 13.7 and 7.8%, and HORRAT(R) values were 0.7 and 0.5. Samples containing residues at the levels of 0.25 and 0.80 ppm were analyzed. Mean recoveries were 73 and 77%, RSD(R) were 12.6 and 10.5%, and HORRAT(R) values were 0.6 and 0.6. The determination limit was 0.1 ppm (oxytetracycline, tetracycline) and 0.02 ppm (chlortetracycline). These results show that this method has good performance.
Fujita
Kazuhiro
K
Japan Food Research Laboratories Nagoya Branch, 4-5-13, Osu, Naka-ku, Nagoya 460-0011, Japan.
Ito
Hironobu
H
Yamaguchi
Takahiro
T
Yoneyama
Satoshi
S
Sengoku
Kiyoki
K
Sumiya
Yoshimichi
Y
Watanabe
Megumi
M
Mizutani
Yuri
Y
Hirose
Tsuguo
T
D000900:Anti-Bacterial Agents / Q000032:analysis*; D001681:Biological Assay / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D007753:Laboratories; D013754:Tetracyclines / Q000032:analysis*; D000099219:Royal Jelly
D000900:Anti-Bacterial Agents; D005227:Fatty Acids; D013754:Tetracyclines; D000099219:Royal Jelly