title: Short-chain fatty acid receptor, GPR43, is expressed by enteroendocrine cells and mucosal mast cells in rat intestine.
abstract: Short-chain fatty acids (SCFAs), such as acetate, propionate, and butyrate, are the major anions in the large intestinal lumen. They are produced from dietary fiber by bacterial fermentation and are known to have a variety of physiological and pathophysiological effects on the intestine. In the present study, we investigated the expression of the SCFA receptor, GPR43, in the rat distal ileum and colon. Expression of GPR43 was detected by reverse transcriptase/polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemistry. mRNA for GPR43 was detected, by RT-PCR, in extracts of the whole wall and separated mucosa from the ileum and colon and from muscle plus submucosa from the ileum, but not from muscle plus submucosa preparations from the colon. We raised a rabbit antiserum against a synthesized fragment of rat GPR43; this was specific for rat GPR43. GPR43 protein was detected by Western blot analysis in extracts of whole wall and separated mucosa, but not in muscle plus submucosa extracts. By immunohistochemistry, GPR43 immunoreactivity was localized to enteroendocrine cells expressing peptide YY (PYY), whereas 5-hydroxytryptamine (5-HT)-immunoreactive (IR) enteroendocrine cells were not immunoreactive for GPR43. Mast cells of the lamina propria expressing 5-HT were also GPR43-IR. The results of the present study suggest that the PYY-containing enteroendocrine cells and 5-HT-containing mucosal mast cells sense SCFAs via the GPR43 receptor. This is consistent with physiological data showing that SCFAs stimulate the release of PYY and 5-HT from the ileum and colon.
authors.affiliation: Laboratory of Physiology, Institute for Environmental Sciences, University of Shizuoka, 52-1 Yada, Shizuoka, 422-8526, Japan.
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Karaki
authors.forename: Retsu
authors.initials: R
authors.lastname: Mitsui
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Hayashi
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiya
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Iwanaga
authors.forename: John B
authors.initials: JB
authors.lastname: Furness
authors.forename: Atsukazu
authors.initials: A
authors.lastname: Kuwahara
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003106:Colon / Q000166:cytology / Q000378:metabolism*; D019858:Enteroendocrine Cells / Q000166:cytology / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D007082:Ileum / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism*; D019894:Peptide YY / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012701:Serotonin / Q000378:metabolism
chemical_list: D005232:Fatty Acids, Volatile; C518630:Ffar2 protein, rat; D043562:Receptors, G-Protein-Coupled; D019894:Peptide YY; D012701:Serotonin