title: Decrease in the incidence of mupirocin resistance among methicillin-resistant Staphylococcus aureus in carriers from an intensive care unit.
abstract: BACKGROUND
  Methicillin-resistant Staphylococcus aureus (MRSA) is a serious nosocomial problem, globally distributed. Decolonization with mupirocin can be used to control its dissemination.
  
  
  OBJECTIVE
  To determine the incidence of mupirocin resistance among MRSA carriers from an intensive care unit.
  
  
  METHODS
  We obtained 2723 nasal swabs during 3 years. Resistance to methicillin and mupirocin were verified (agar diffusion and the E test) and confirmed by polymerase chain reaction (PCR) (mecA for methicillin; ileS-2 and mupA for mupirocin). Plasmid-curing procedure and pulsed-field gel electrophoresis (PFGE) were employed in isolates exhibiting high resistance to mupirocin (HR-Mup) and in other selected organisms.
  
  
  RESULTS
  The overall incidence of HR-Mup among MRSA carriers during the studied period was 4.84% (8/165); however, the incidence decreased from 13.04% (6/46) in the first year to 3.5% (2/57) in the second year and was 0% in the last year (P = .02). LR-Mup, in contrast, increased significantly (P = .01).
  
  
  CONCLUSIONS
  Plasmid-curing procedure showed the plasmid location of genes responsible for HR-Mup. PFGE demonstrated that most MRSA, including the isolates with HR-Mup, were genetically related. The decline in HR-Mup may be attributable to the plasmid location of genes (ileS-2/mupA) and to the fact that all patients colonized with HR-Mup MRSA died or were discharged in a relatively short period of time.
authors.affiliation: Fundação Faculdade Federal de Ciências Médicas de Porto Alegre.
authors.forename: Juliana
authors.initials: J
authors.lastname: Caierão
authors.forename: Laura
authors.initials: L
authors.lastname: Berquó
authors.forename: Cícero
authors.initials: C
authors.lastname: Dias
authors.forename: Pedro Alves
authors.initials: PA
authors.lastname: d'Azevedo
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002353:Carrier State / Q000382:microbiology*; D003428:Cross Infection / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D015994:Incidence; D007362:Intensive Care Units; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D016712:Mupirocin / Q000494:pharmacology*; D009666:Nose / Q000382:microbiology; D009687:Nuclear Proteins / Q000235:genetics; D046915:Penicillin-Binding Proteins; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009687:Nuclear Proteins; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; C480380:mupA protein, Staphylococcus aureus; D016712:Mupirocin