title: Nuclear and cytoplasmic localization of interferon-tau in in vitro-produced bovine blastocysts.
abstract: Experiments were conducted to detect interferon-tau in bovine in vitro-derived blastocysts by transmission electron (TEM) and confocal microscopy. TEM showed the presence of IFN-tau in the cytoplasm and the nuclei of expanded blastocysts. Confocal microscopy similarly confirmed the presence of IFN-tau in the trophectoderm of blastocysts. The distribution of IFN-tau appeared variable with some cells showing strong labeling while others appeared to be devoid of the protein.
authors.affiliation: Division of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA 70433, USA.
authors.forename: Kelly M
authors.initials: KM
authors.lastname: Johnson
authors.forename: Xavier
authors.initials: X
authors.lastname: Alvarez
authors.forename: Olga N
authors.initials: ON
authors.lastname: Borkhsenious
authors.forename: H Michael
authors.initials: HM
authors.lastname: Kubisch
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D002417:Cattle / Q000196:embryology*; D046149:Embryo Culture Techniques / Q000662:veterinary; D005260:Female; D007370:Interferon Type I / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000662:veterinary; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D011257:Pregnancy Proteins / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D007370:Interferon Type I; D011257:Pregnancy Proteins; C020947:interferon tau