Antioxidant property of an active component purified from the leaves of paraquat-tolerant Rehmannia glutinosa.
Acteoside extracted from the leaves of Rehmannia glutinosa was examined to determine the mechanism(s) of its antioxidant properties. The deoxyribose assay system showed that acteoside has a high redox potential as electron donor, which generates hydroxyl radicals in an Fe3+-dependent manner similar to ascorbic acid. However, the antioxidant properties of acteoside differ from those of ascorbic acid in that the superoxide anion-mediated reduction of nitroblue tetrazolium was actively inhibited by acteoside but not by ascorbic acid. Acteoside protected cells against glucose oxidase-mediated cytotoxicity and apoptosis in a dose-dependent manner. In addition, acteoside had immune stimulating effects, as shown by the acteoside-mediated increase in the level of DNA synthesis, viability, and cytokine secretion in mouse splenocytes. Moreover, acteoside inhibited the gelatinolytic activity of MMP proteins in a dose-dependent manner. Considering these results and the fact that acteoside is a water-soluble natural product, acteoside might have potential as a preventative treatment for oxidative stress-mediated diseases and have possibilities in the cosmetic industry.
Kim
So-Soon
SS
Laboratory of Cell Biology, Department of Orthodontics, Institute of Oral Biosciences, Chonbuk National University, Chonju, Korea.
Son
Young-Ok
YO
Chun
Jae-Chul
JC
Kim
Sung-Eun
SE
Chung
Gook-Hyun
GH
Hwang
Ki-Jun
KJ
Lee
Jeong-Chae
JC
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000737:chemistry; D003855:Deoxyribose; D006540:Herbicides / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D009580:Nitroblue Tetrazolium / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology*; D010936:Plant Extracts / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects*; D032267:Rehmannia / Q000378:metabolism*; D013481:Superoxides / Q000737:chemistry
D000975:Antioxidants; D006540:Herbicides; D010936:Plant Extracts; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D003855:Deoxyribose; D010269:Paraquat; D001205:Ascorbic Acid