[Flow cytometry analysis and differentiation study of selected nestin positive cells].
OBJECTIVE
To verify the hypothesis that selected nestin positive cells derived from human fetal pancreas (according as medical ethnics) have surface markers similar to bone marrow mesenchymal stem cells (MSCs), and that these cells have multilineage potential.


METHODS
The cell surface markers were determined by flow cytometry, and then the potential that these cells might be differentiated into adipocytes and osteoplasts were explored.


RESULTS
These cells have similar surface markers as MSCs of bone marrow origin. These cells was induced to differentiate into adipocytes and osteoplasts.


CONCLUSIONS
Selected nestin positive cells derived from human fetal pancreas have certain characteristics of MSCs.
Wang
Hong
H
Stem Cell Research Center, Peking University Health Science Center, Beijing 100083, China.
Hu
Jiang
J
Li
Ling-song
LS
Hong
Tian-pei
TP
Li
Li-ying
LY
D017667:Adipocytes / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D053686:Fetal Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007381:Intermediate Filament Proteins; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D009419:Nerve Tissue Proteins; D064231:Nestin; D010179:Pancreas / Q000166:cytology / Q000196:embryology
D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin