Spectrophotometric estimation of functional groups on microslides for preparation of biochips.
A universal reagent 1-O-(4,4'-dimethoxytrityl)-6-aminohexanol (DTAH) is described for the estimation of surface-bound functionalities (epoxy, aldehyde, and carboxyl) required for preparation of oligonucleotide arrays (biochips). The method involves the reaction of universal reagent DTAH with surface-bound functionality under microwaves for 10 min, followed by washings to remove the excess reagent. In the subsequent step, a weighed amount of DTAH-treated surface is exposed to acid to liberate 4,4'-dimethoxytrityl cation, which is measured at 505 nm to determine the functional group loading on the surface.
Mahajan
Shweta
S
Nucleic Acids Research Laboratory, Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, Delhi 110 007, India.
Garg
Angad
A
Goel
Mayurika
M
Kumar
P
P
Gupta
K C
KC
D000447:Aldehydes / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis; D004852:Epoxy Compounds / Q000032:analysis; D000441:Hexanols / Q000138:chemical synthesis / Q000737:chemistry; D007700:Kinetics; D008872:Microwaves; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods*; D014320:Triphenylmethyl Compounds / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry
C511126:1-O-(4,4'-dimethoxytrityl)-6-aminohexanol; D000447:Aldehydes; D002264:Carboxylic Acids; D004852:Epoxy Compounds; D000441:Hexanols; D014320:Triphenylmethyl Compounds; C060949:4,4'-dimethoxytrityl cation