title: [Detection of Chlamydia infection and estimation of immune reactivity in sportsmen by blood leukocyte culture method].
abstract: New method of the diagnosis and cultivation of Chlamydia trachomatis using peripheral blood leukocyte cultures is worked out and implemented in the clinical practice. This method was used for the diagnosis of asymptomatic chlamydial infection in sportsmen. Among 48 sportsmen Chlamydia trachomatis was diagnosed in 28 persons. Morbidity in these sportsmen was significantly higher, than in sportsmen without chlamydial infection. Number of macrophage-lymphocyte rosettes in leukocyte cultures that reflects immune reactivity of organism was significantly low in sportsmen with Chlamydia trachomatis than in the group without this infection. Results of our studies showed that leukocyte culture method is the most useful and reliable for the detection of latent or asymptomatic Chlamydia trachomatis infection. The diagnosis of this infection in sportsmen helps to reveal the reason of different clinical symptoms to carry out the appropriate regimen of treatment. Microphotographs of Chlamydia trachomatis colonies in the blood leukocyte culture and electron microphotographs of different stages of chlamydial life cycle in the host cell in vitro are presented.
authors.forename: T G
authors.initials: TG
authors.lastname: Saralidze
authors.forename: O T
authors.initials: OT
authors.lastname: Vyrubov
authors.forename: K G
authors.initials: KG
authors.lastname: Kiladze
authors.forename: L A
authors.initials: LA
authors.lastname: Mokhevishvili
authors.forename: G N
authors.initials: GN
authors.lastname: Saralidze
mesh_terms: D000293:Adolescent; D000328:Adult; D002690:Chlamydia Infections / Q000097:blood / Q000276:immunology / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000276:immunology* / Q000302:isolation & purification*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007962:Leukocytes / Q000276:immunology* / Q000382:microbiology*; D008297:Male; D013177:Sports