title: Evaluation of a bi-layer wound dressing for burn care. II. In vitro and in vivo bactericidal properties.
abstract: We have recently designed a medicated bi-layer wound dressing to address the key requirements for treating external, contaminated war wounds. This study assessed the in vitro and in vivo bactericidal efficacies of the DRDC hydrogel/polyurethane wound dressing. Chloramphenicol- and chlorhexidine-loaded DRDC dressings produced significantly larger zones of inhibition against Pseudomonas aeruginosa than the other medicated dressings for 4 d. Chlorhexidine-loaded Allevyn and Hydrasorb remained bactericidal for 48 h only. Chloramphenicol-loaded Hydrasorb and Allevyn remained bactericidal for 1 and 3 d, respectively. Ps. aeruginosa and Staphylococcus epidermidis counts in wounds treated with chlorhexidine- and chloramphenicol-loaded DRDC dressings for 24 h were 1-3-log lower than those of control wounds. While Ps. aeruginosa counts in the wounds on day 4 were comparable following daily changes of either antiseptic-loaded dressings, chlorhexidine showed a 75% greater bactericidal efficacy against Staph. epidermidis than chloramphenicol. Though increasing the frequency of dressing changes led to a greater reduction in the wound bacterial load, the contamination levels of all antiseptic-treated wounds remained below 10(5) CFU/g of wound. Cerium nitrate-loaded dressings did not exert any bactericidal effect, irrespective of the experimental conditions. These data show that the DRDC dressing is effective in delivering medications, such as an antimicrobial agent, to the wound bed.
authors.affiliation: Operational Medicine Section, Trauma Group, Defence Research and Development Canada Toronto, 1133 Sheppard Avenue West, Toronto, Ont., Canada M3M 3B9. lucie.martineau@drdc-rddc.gc.ca
authors.forename: Lucie
authors.initials: L
authors.lastname: Martineau
authors.forename: Pang N
authors.initials: PN
authors.lastname: Shek
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage*; D001458:Bandages; D002056:Burns / Q000382:microbiology*; D002701:Chloramphenicol / Q000008:administration & dosage; D002710:Chlorhexidine / Q000008:administration & dosage; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage; D008297:Male; D011552:Pseudomonas Infections / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013552:Swine; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects; D014946:Wound Infection / Q000517:prevention & control*
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002701:Chloramphenicol; D002710:Chlorhexidine