title: Genotoxicity and cytotoxicity of mineral trioxide aggregate and regular and white Portland cements on Chinese hamster ovary (CHO) cells in vitro.
abstract: OBJECTIVE
  Recently, mineral trioxide aggregate (MTA) and Portland cement have been used in dentistry as root-end-filling materials. However, the reported results concerning the biocompatibility of these materials are inconsistent. The goal of this study was to examine the genotoxicity and cytotoxicity of MTA and Portland cements in vitro by the single-cell gel (comet) assay and trypan blue exclusion test.
  
  
  METHODS
  Chinese hamster ovary (CHO) cells were exposed to MTA and regular and white Portland cements at final concentration ranging from 1 to 1000 microg/mL for 1 h at 37 degrees C.
  
  
  RESULTS
  All compounds tested did not show genotoxic effects in all concentrations evaluated. No significant differences (P > .05) in cytotoxicity were observed for all compounds tested.
  
  
  CONCLUSIONS
  Taken together, our results suggest that MTA and Portland cements are not genotoxins and are not able to induce cellular death.
authors.affiliation: Department of Pathology, Botucatu Medical School, Botucatu, Brazil. ak92@hotmail.com
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
authors.forename: Marina Mariko
authors.initials: MM
authors.lastname: Sugui
authors.forename: Mariza Akemi
authors.initials: MA
authors.lastname: Matsumoto
authors.forename: Marco Antonio Húngaro
authors.initials: MA
authors.lastname: Duarte
authors.forename: Mariangela Esther Alencar
authors.initials: ME
authors.lastname: Marques
authors.forename: Daisy Maria Favero
authors.initials: DM
authors.lastname: Salvadori
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D016466:CHO Cells / Q000187:drug effects; D017610:Calcium Compounds / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D003738:Dental Cements / Q000633:toxicity; D004338:Drug Combinations; D010087:Oxides / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity; D018709:Statistics, Nonparametric; D014343:Trypan Blue
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004396:Coloring Agents; D003738:Dental Cements; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C472247:accelerated Portland cement; C086631:mineral trioxide aggregate; D014343:Trypan Blue