Comprehensive protein profiling by multiplexed capillary zone electrophoresis using cross-linked polyacrylamide coated capillaries.
We have recently developed a new process to create cross-linked polyacrylamide (CPA) coatings on capillary walls to suppress protein-wall interactions. Here, we demonstrate CPA-coated capillaries for high-efficiency (>2 x 10(6) plates per meter) protein separations by capillary zone electrophoresis (CZE). Because CPA virtually eliminates electroosmotic flow, positive and negative proteins cannot be analyzed in a single run. A "one-sample-two-separation" approach is developed to achieve a comprehensive protein analysis. High throughput is achieved through a multiplexed CZE system.
Liu
Shaorong
S
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409, USA. Shaorong.liu@ttu.edu
Gao
Lin
L
Pu
Qiaosheng
Q
Lu
Joann J
JJ
Wang
Xingjia
X
D000180:Acrylic Resins / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D011485:Protein Binding; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D000099166:Polyacrylamides
D000180:Acrylic Resins; D003432:Cross-Linking Reagents; D011506:Proteins; D000099166:Polyacrylamides