title: Sensitivity to cisplatin determined by the histoculture drug response assay and clinical response of endometrial cancer.
abstract: This study investigated the value of the in vitro histoculture drug response assay (HDRA) for predicting the efficacy of chemotherapy in patients with endometrial cancer. Specimens were obtained from 115 patients with endometrial cancer treated at Keio University Hospital between 1994 and 2002. Tumor fragments were cultured on collagen sponge gel with cisplatin for 7 days, and cell viability was assessed. The cutoff value of the 50% inhibitory concentration of cisplatin was set at 23 microg/mL. Sensitivity of stage III or IV disease to chemotherapy was investigated, and differences of 5-year progression-free survival between patients with sensitive and resistant tumors were evaluated by the Kaplan-Meier method. Tumors were evaluable in 93.0% of patients (107/115). Among 38 patients in stages III or IV, 23 received chemotherapy containing cisplatin. Seven sensitive tumors did not recur, while recurrence/progression occurred within 6 months in 8/16 patients with tumors showing low sensitivity. Among stages III and IV patients, there was a significant difference of 5-year progression-free survival (P < 0.05) between those with tumors showing high or low sensitivity. Accordingly, the HDRA may predict the efficacy of chemotherapy for endometrial cancer.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kanasugi
authors.forename: D
authors.initials: D
authors.lastname: Aoki
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: N
authors.initials: N
authors.lastname: Susumu
authors.forename: S
authors.initials: S
authors.lastname: Nakata
authors.forename: M
authors.initials: M
authors.lastname: Horiuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Udagawa
authors.forename: S
authors.initials: S
authors.lastname: Nozawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D016889:Endometrial Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011336:Probability; D011379:Prognosis; D012189:Retrospective Studies; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D046509:Tissue Culture Techniques; D016896:Treatment Outcome
chemical_list: D002945:Cisplatin