title: Biological function of ;pathogenesis-related' proteins: four PR proteins of tobacco have 1,3-beta-glucanase activity.
abstract: Three of the ten acidic ;pathogenesis-related' (PR) proteins known to accumulate in Nicotiana tabacum cv Samsun NN reacting hypersensitively to tobacco mosaic virus, namely -O, -N and -2, have been shown to have 1,3-beta-glucanase (EC 3.2.1.39) activity. By using sera raised against each protein purified to homogeneity close serological relationships have been demonstrated between the three proteins. The same specific sera cross-reacted with a basic protein which is also a 1,3-beta-glucanase induced by virus infection and which can be considered as a new basic pathogenesis-related protein of tobacco. Protein PR-O and the basic 1,3-beta-glucanase display about the same specific enzymatic activity, i.e. 50-fold and 250-fold higher than specific activities of proteins PR-N and -2 respectively.
authors.affiliation: Institut de Biologie Moléculaires des Plantes, 12, rue du Général Zimmer, 67000 Strasbourg, France.
authors.forename: S
authors.initials: S
authors.lastname: Kauffmann
authors.forename: M
authors.initials: M
authors.lastname: Legrand
authors.forename: P
authors.initials: P
authors.lastname: Geoffroy
authors.forename: B
authors.initials: B
authors.lastname: Fritig