Biocompatibility testing of novel multifunctional polymeric biomaterials for tissue engineering applications in head and neck surgery: an overview.
Biomaterial research and tissue engineering are rapidly growing scientific fields that need an interdisciplinary approach where clinicians should be included from the onset. Biocompatibility testing in vitro and in vivo comprise the agarose-overlay test, the MTT test, direct cell seeding tests and the chorioallantoic membrane test for angiogenic effects, among others. Molecular biology techniques such as real-time polymerase chain reaction and microarray technology facilitate the investigation of tissue integration into biomaterials on a cellular and molecular level. The physicochemical characterization of biomaterials is conducted using such methods as X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Excellent biocompatibility and biofunctionality were demonstrated for a series of recently developed multifunctional biodegradable, polymeric biomaterials both in vitro and in vivo. Novel, multifunctional polymeric biomaterials offer a highly specific adjustment to the physiological, anatomical and surgical requirements and can thereby facilitate new therapeutic options in head and neck surgery.
Rickert
Dorothee
D
Department of Otolaryngology and Head and Neck Surgery, University of Ulm, Ulm, Germany. Dorothee.Rickert@medizin.uni-ulm.de
Lendlein
Andreas
A
Peters
Ilka
I
Moses
Marsha A
MA
Franke
Ralf-Peter
RP
D020341:Absorbable Implants; D000818:Animals; D006801:Humans; D008422:Materials Testing / Q000379:methods*; D013517:Otorhinolaryngologic Surgical Procedures; D011108:Polymers / Q000627:therapeutic use; D023822:Tissue Engineering
D011108:Polymers