title: [West Nile virus infection: serological investigation among horses in France and in Africa].
abstract: This study was carried out in 2003 to detected serological evidence of West Nile virus infection in 190 Army horses kept nearby French troops stationed in Southeast France and in Africa (Chad, Côte d'Ivoire and Senegal). Both IgG and IgM antibodies were searched for using an ELISA assay. Specifiity of IgG antibodies was determined by western blot and plaque reduction seroneutraization. Finding showed that 79% of the Army horses (n=96) tested in Africa presented specific IgG antibodies. All horses that were seropositive for IgG were seronegative for IgM. None of the Army horses (n=94) tested in the Southeast France were seropositive for West Nile virus. This study indicates that West Nile virus has circulated in all three African countries but not recently. It also underscores the value of western blotting as a rapid, specific confirmation technique that could eliminate the need to use plaque reduction seroneutralization.
authors.affiliation: Cellule vétérinaire des Eléments Français au Tchad, N'Djamena, Tchad. sertemarco@wanadoo.fr
authors.forename: O
authors.initials: O
authors.lastname: Cabre
authors.forename: J P
authors.initials: JP
authors.lastname: Durand
authors.forename: A
authors.initials: A
authors.lastname: Prangé
authors.forename: J
authors.initials: J
authors.lastname: Gomez
authors.forename: L
authors.initials: L
authors.lastname: Maurizi
authors.forename: H
authors.initials: H
authors.lastname: Tolou
authors.forename: B
authors.initials: B
authors.lastname: Davoust
mesh_terms: D000349:Africa; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005602:France; D006734:Horse Diseases / Q000097:blood / Q000635:transmission / Q000821:virology*; D006736:Horses; D007074:Immunoglobulin G / Q000032:analysis; D012698:Serologic Tests; D014901:West Nile Fever / Q000097:blood / Q000635:transmission / Q000662:veterinary*; D014902:West Nile virus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G