title: Murine fetal small-intestine grafts: morphometric and immunohistochemical evaluation.
abstract: We investigated histopathological changes following murine fetal intestinal transplantation. Fetal intestine, obtained from a pregnant C57BL/6 mouse, was transplanted into BALB/c and C57Bl/6 mice. Recipients were divided into three groups: isogeneic, and allogeneic treated with 3 mg/kg/day gangliosides (Allo-a) or 9 mg/kg/day (Allo-b). One week after transplant, all grafts showed good viability, confirmed by cellular mitosis in the mucosa and a well-defined propria muscular layer. Isogeneic grafts showed a thicker muscular layer than in the Allo-a (P = 0.02) and Allo-b (P = 0.004) groups. There was no difference in number of mitotic cells among groups. Goblet cells were significantly reduced in allografts treated with 3 mg gangliosides (P = 0.013) or 9 mg gangliosides (P = 0.002) compared to isografts. Villi height was similar in all studied groups. There was no difference in positivity of the enteric nervous system among groups. Atrophy was more common in the allogeneic groups, suggesting that isografts had better development than allografts treated with gangliosides. (
authors.affiliation: Department of Surgery, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Carlos Eduardo Saldanha
authors.initials: CE
authors.lastname: de Almeida
authors.forename: Kátia Ferreira
authors.initials: KF
authors.lastname: de Oliveira
authors.forename: Mônica Cecília Bochetti
authors.initials: MC
authors.lastname: Manna
authors.forename: Ricardo
authors.initials: R
authors.lastname: Artigiani
authors.forename: Márcia Kiyomi
authors.initials: MK
authors.lastname: Koike
authors.forename: Edna Frasson de Souza
authors.initials: EF
authors.lastname: Montero
mesh_terms: D000818:Animals; D005260:Female; D016332:Fetal Tissue Transplantation / Q000276:immunology / Q000473:pathology*; D005732:Gangliosides / Q000502:physiology*; D006085:Graft Survival; D007421:Intestine, Small / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009418:S100 Proteins / Q000378:metabolism; D014184:Transplantation, Homologous
chemical_list: D005732:Gangliosides; D009418:S100 Proteins