Proteome analysis of rat liver mitochondria reveals a possible compensatory response to endotoxic shock.
Organ failure induced by endotoxic shock has recently been associated with affected mitochondrial function. In this study, effects of in vivo lipopolysaccharide-challenge on protein patterns of rat liver mitochondria in treated animals versus controls were studied by two-dimensional electrophoresis (differential image gel electrophoresis). Significant upregulation was found for ATP-synthase alpha chain and superoxide dismutase [Mn]. Our data suggest that endotoxic shock mediated changes in the mitochondrial proteome contribute to a compensatory reaction (adaptation to endotoxic shock) rather than to a mechanism of cell damage.
Miller
Ingrid
I
Institute of Medical Chemistry, Department of Natural Sciences, University of Veterinary Medicine Vienna, Austria.
Gemeiner
Manfred
M
Gesslbauer
Bernd
B
Kungl
Andreas
A
Piskernik
Christina
C
Haindl
Susanne
S
Nürnberger
Silvia
S
Bahrami
Soheyl
S
Redl
Heinz
H
Kozlov
Andrey V
AV
D000818:Animals; D019069:Cell Respiration; D015180:Electrophoresis, Gel, Two-Dimensional; D008070:Lipopolysaccharides / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D010100:Oxygen / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D012772:Shock, Septic / Q000235:genetics / Q000378:metabolism*
D008070:Lipopolysaccharides; D020543:Proteome; D017382:Reactive Oxygen Species; D010100:Oxygen