title: Determination of ranitidine in urine by capillary electrophoresis-electrochemiluminescent detection.
abstract: The fast analysis of ranitidine is of clinical importance in understanding its efficiency and a patient's treatment history. In this paper, a novel determination method for ranitidine based on capillary electrophoresis-electrochemiluminescence detection is described. The conditions affecting separation and detection were investigated in detail. End-column detection of ranitidine in 5 mM Ru(bpy)(3)(2+) solution at applied voltage of 1.20 V was performed. Favorable ECL intensity with higher column efficiency was achieved by electrokinetic injection for 10s at 10 kV. The R.S.D. values of ECL intensity and migration time were 6.38 and 1.84% for 10(-4) M and 6.01 and 0.60% for 10(-5) M, respectively. A detection limit of 7 x 10(-8) M (S/N=3) was achieved. The proposed method was applied satisfactorily to the determination of ranitidine in urine in 6 min.
authors.affiliation: Department of Chemistry, Tianjin University, Tianjin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.forename: Yiling
authors.initials: Y
authors.lastname: Tian
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Sun
authors.forename: Xue-Bo
authors.initials: XB
authors.lastname: Yin
authors.forename: Qian
authors.initials: Q
authors.lastname: Xiang
authors.forename: Ge
authors.initials: G
authors.lastname: Ma
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D002021:Buffers; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006635:Histamine H2 Antagonists / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D011899:Ranitidine / Q000652:urine*
chemical_list: D002021:Buffers; D006635:Histamine H2 Antagonists; D011899:Ranitidine