Feeding high levels of lupine seeds to broiler chickens: plasma micronutrient status in the context of digesta viscosity and morphometric and ultrastructural changes in the gastrointestinal tract.
This work examines the effects of lupine-based diets on the status of the host's riboflavin and zinc. Test diets contained 35% soybean meal (control) or raw (40%) or dehulled (35%) lupine seed meal and were isocaloric (13.4 MJ of AME/kg) and isonitrogenous (23% crude protein). Each diet was offered ad libitum to a group of 16 male commercial broiler chicks for 21 d, starting at 1 d of age. Broilers fed lupine diets had lower feed intakes and growth rates. All sections of the intestinal tract were significantly enlarged (P < 0.01) in all groups fed lupine-based diets in comparison with broilers fed the soybean meal diet, but there were no differences in the morphologies of the mucosa, submucosa, muscularis, or serosa. Increased size of the duodenum, jejunum, and ileum was predominantly attributed to the increase in length rather than intestinal tissue mass. Hence, the enlargement of the intestinal tract was consistent with physiological hyperplasia and not pathological remodeling and hypertrophy. Overall, broilers fed lupine-based diets had more viscous digesta than those fed the soybean meal diet, but the differences were statistically not significant. Blood plasma Zn concentration did not differ between broilers fed lupine-based diets and those fed soybean-meal-based diets, and all broilers fed lupine-based diets had significantly higher (P < 0.001) riboflavin concentrations. In this context, it is apparent that the bioavailability of these micronutrients from lupine diets is not compromised. Intestinal tissue hyperplasia may be interpreted as physiological adaptation to increase absorptive capacity and thus maximize absorption of essential nutrients in the face of antinutritional factors in the diet.
Olkowski
B I
BI
Department of Animal and Poultry Science, 51 Campus Drive, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5A8. olkowski@sask.usask.ca
Classen
H L
HL
Wojnarowicz
C
C
Olkowski
A A
AA
D000821:Animal Feed; D000818:Animals; D002645:Chickens; D004032:Diet; D005766:Gastrointestinal Contents / Q000737:chemistry / Q000187:drug effects*; D041981:Gastrointestinal Tract / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure*; D031297:Lupinus / Q000737:chemistry*; D008297:Male; D018977:Micronutrients / Q000097:blood*; D028321:Plant Preparations / Q000008:administration & dosage* / Q000494:pharmacology*; D012256:Riboflavin / Q000032:analysis / Q000097:blood; D012639:Seeds / Q000737:chemistry*; D014783:Viscosity / Q000187:drug effects; D015032:Zinc / Q000032:analysis / Q000097:blood
D018977:Micronutrients; D028321:Plant Preparations; D015032:Zinc; D012256:Riboflavin