Interleukin-8 is associated with circulating CD133+ progenitor cells in acute myocardial infarction.
OBJECTIVE
Release of progenitor cells is observed during inflammatory conditions and contributes to neovascularization. We, therefore, sought to investigate the relationship of circulating progenitor cells and interleukin (IL)-8 in acute myocardial infarction (AMI).


RESULTS
From patients with stable angina and AMI, serial venous blood samples were obtained. The number of circulating CD133+CD45- progenitor cells, endothelial progenitor cells (EPCs), and circulating endothelial P1H12+CD45- cells was analyzed by flow cytometry. After stenting in patients with AMI, an increase in plasma IL-8 and vascular endothelial growth factor (VEGF) concentrations was observed, which was only minimal in patients with stable angina. Only in patients with AMI, this was followed by an increase in circulating CD133+CD45- progenitor cells. In contrast, circulating endothelial P1H12+CD45- cells and E-selectin RNA expression in peripheral blood were only elevated early in AMI, indicating shedding of activated endothelial cells. Multivariable analysis revealed an association of IL-8 and circulating CD133+CD45- progenitor cells in AMI, in addition to statin therapy and risk factor profile.


CONCLUSIONS
In AMI, IL-8 is associated with circulating progenitor cells. In addition to the pro-angiogenic functions of IL-8 and VEGF, this mechanism may contribute to new vessel generation and, thereby, improve myocardial function.
Schömig
Kathrin
K
Deutsches Herzzentrum und 1. Medizinische Klinik der Technischen Universität München, Germany.
Busch
Gabriele
G
Steppich
Birgit
B
Sepp
Dominik
D
Kaufmann
Jan
J
Stein
Andreas
A
Schömig
Albert
A
Ott
Ilka
I
D000071916:AC133 Antigen; D000787:Angina Pectoris / Q000097:blood / Q000473:pathology; D015703:Antigens, CD; D042783:Endothelial Cells / Q000473:pathology; D005434:Flow Cytometry; D006023:Glycoproteins; D006801:Humans; D016209:Interleukin-8 / Q000097:blood / Q000502:physiology*; D009203:Myocardial Infarction / Q000097:blood / Q000473:pathology*; D010455:Peptides; D013234:Stem Cells; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D016209:Interleukin-8; C000606023:PROM1 protein, human; D010455:Peptides; D042461:Vascular Endothelial Growth Factor A