title: Luteolin inhibits LPS-stimulated inducible nitric oxide synthase expression in BV-2 microglial cells.
abstract: Overproduction of nitric oxide (NO) by inducible nitric oxide synthase (iNOS) acts as a neurotoxic effector in the central nervous system, resulting in neurodegenerative diseases. From the alcoholic extracts of Perilla frutescens, we have purified an inhibitor of NO production in lipopolysaccharide (LPS)-activated microglia by activity-guided purification. The active compound was identified as luteolin by spectral analysis. Luteolin inhibited the NO production in LPS-activated microglia in a dose-dependent manner (IC50=6.9 microM). Luteolin also suppressed the degradation of I-kappaB-alpha, the expression of protein and mRNA of iNOS in LPS-activated microglia as observed in Western blot analysis and RT-PCR experiments. Luteolin may have beneficial effects in the treatment of neuro-inflammatory diseases through the inhibition of iNOS expression.
authors.affiliation: College of Pharmacy, Sookmyung Women's University, Seoul, Korea.
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Hwa Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Ming Hong
authors.initials: MH
authors.lastname: Lee
authors.forename: Jaeyeon
authors.initials: J
authors.lastname: Kim
authors.forename: Changbae
authors.initials: C
authors.lastname: Jin
authors.forename: Jae-Ha
authors.initials: JH
authors.lastname: Ryu
mesh_terms: D000818:Animals; D002460:Cell Line; D008070:Lipopolysaccharides; D047311:Luteolin / Q000032:analysis / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000187:drug effects*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D031344:Perilla frutescens / Q000737:chemistry*; D014176:Protein Biosynthesis / Q000187:drug effects
chemical_list: D008070:Lipopolysaccharides; D019001:Nitric Oxide Synthase; D047311:Luteolin