DNA methylation, histone H3 methylation, and histone H4 acetylation in the genome of a crustacean.
In this work, we used antibodies against histone H3 trimethylated at lysine 9 (H3K9m3); against histone H4 acetylated at lysines 5, 8, 12, and 16 (H4ac); and against DNA methylated at 5C cytosine (m5C) to study the presence and distribution of these markers in the genome of the isopod crustacean Asellus aquaticus. The use of these 3 antibodies to immunolabel spermatogonial metaphases yields reproducible patterns on the chromosomes of this crustacean. The X and Y chromosomes present an identical banding pattern with each of the antibodies. The heterochromatic telomeric regions and the centromeric regions are rich in H3K9m3, but depleted in m5C and H4ac. Thus, m5C does not seem to be required to stabilize the silence of these regions in this organism.
Barzotti
Rita
R
Dipartimento de Genetica e Biologia Molecolare, Università La Sapienza, Roma, Italia.
Pelliccia
Franca
F
Rocchi
Angela
A
D000107:Acetylation; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002503:Centromere / Q000737:chemistry / Q000378:metabolism; D019175:DNA Methylation; D016678:Genome; D006657:Histones / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D033321:Isopoda / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D008745:Methylation; D013093:Spermatogonia / Q000378:metabolism; D016615:Telomere / Q000737:chemistry / Q000378:metabolism; D014960:X Chromosome / Q000737:chemistry / Q000378:metabolism; D014998:Y Chromosome / Q000737:chemistry / Q000378:metabolism
D000906:Antibodies; D006657:Histones