Identification of the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFcgamma2R) using synthetic peptides.
To identify the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFcgamma2R), peptides derived from the membrane-distal extracellular domain (EC1) of boFcgamma2R corresponding to the homologous region of human FcalphaRI were synthesized. Binding of bovine IgG2 to the different peptides was tested by Dot-blot assay, and the peptide showing maximal binding was further modified by truncation and mutation. The minimum effective peptide 82FIGV85 located in the putative F-G loop of the EC1 domain was found to bind bovine IgG2 specifically and inhibit the binding of bovine IgG2 to the receptor. The Phe82, Ile83 and Val85 residues within the linear epitope were shown to be critical for IgG2-binding. Such functional epitope peptide should be very useful for understanding the IgG-Fcgamma interaction and development of FcR-targeting drugs.
Zhang
Gaiping
G
Henan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Guo
Junqing
J
Zhou
Jiyong
J
Wang
Xuannian
X
Li
Qingmei
Q
Yang
Yanyan
Y
Shen
Huigang
H
Zhao
Dong
D
Zhang
Hua
H
Xi
Jun
J
Wang
Li
L
Qiao
Songlin
S
Jin
Xin
X
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D015703:Antigens, CD / Q000276:immunology; D002417:Cattle; D018604:Epitope Mapping / Q000379:methods*; D000939:Epitopes / Q000737:chemistry*; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology* / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D011961:Receptors, Fc / Q000276:immunology; D017452:Receptors, IgG / Q000276:immunology* / Q000378:metabolism
D015703:Antigens, CD; D000939:Epitopes; C036832:Fc(alpha) receptor; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D010446:Peptide Fragments; D011961:Receptors, Fc; D017452:Receptors, IgG