title: Presence of antibodies to non-structural proteins of foot-and-mouth disease virus in repeatedly vaccinated cattle.
abstract: For the purpose of removing infected animals by detecting humoral immune responses to non-structural proteins of the foot-and-mouth disease (FMD) virus, antibodies induced by contaminated residual non-structural proteins contained in less pure FMD vaccine can be problematic for serological screening. The aim of the present study was to measure the possible presence of antibodies against these non-structural proteins in repeatedly vaccinated calves and beef cattle. Five imported FMD vaccines were examined using two commercial ELISA kits, UBI FMDV NS EIA and Ceditest FMDV-NS, for serological testing. After five doses of vaccination, the serum of one calf tested positive, and two vaccines induced a significant increase in anti-3ABC antibodies in calves. This finding demonstrated that a positive reaction to non-structural proteins due to impurities in the FMD vaccine was detectable using commercial tests. A low percentage of field sera sampled from beef cattle in Kinmen also tested positive, but the key factor resulting in the positive reactions could not be positively identified based on our data.
authors.affiliation: Animal Health Research Institute, Council of Agriculture, 376 Chung-Cheng Road, Tamsui, Taipei County 25158, Taiwan. fanlee@mail.nvri.gov.tw
authors.forename: Fan
authors.initials: F
authors.lastname: Lee
authors.forename: Ming-Hwa
authors.initials: MH
authors.lastname: Jong
authors.forename: Der-Wei
authors.initials: DW
authors.lastname: Yang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology* / Q000302:isolation & purification; D009500:Neutralization Tests / Q000662:veterinary; D011897:Random Allocation; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines