title: Gene expression in human small intestinal mucosa in vivo is mediated by iron-induced oxidative stress.
abstract: Iron-induced oxidative stress in the small intestine may alter gene expression in the intestinal mucosa. The present study aimed to determine which genes are mediated by an iron-induced oxidative challenge in the human small intestine. Eight healthy volunteers [22 yr(SD2)] were tested on two separate occasions in a randomized crossover design. After duodenal tissue sampling by gastroduodenoscopy, a perfusion catheter was inserted orogastrically to perfuse a 40-cm segment of the proximal small intestine with saline and, subsequently, with either 80 or 400 mg of iron as ferrous gluconate. After the intestinal perfusion, a second duodenal tissue sample was obtained. Thiobarbituric acid-reactive substances, an indicator of lipid peroxidation, in intestinal fluid samples increased significantly and dose dependently at 30 min after the start of perfusion with 80 or 400 mg of iron, respectively (P < 0.001). During the perfusion with 400 mg of iron, the increase in thiobarbituric acid-reactive substances was accompanied by a significant, momentary rise in trolox equivalent antioxidant capacity, an indicator of total antioxidant capacity (P < 0.05). The expression of 89 gene reporters was significantly altered by both iron interventions. Functional mapping showed that both iron dosages mediated six distinct processes. Three of those processes involved G-protein receptor coupled pathways. The other processes were associated with cell cycle, complement activation, and calcium channels. Iron administration in the small intestine induced dose-dependent lipid peroxidation and a momentary antioxidant response in the lumen, mediated the expression of at least 89 individual gene reporters, and affected at least six biological processes.
authors.affiliation: Department of Internal Medicine/Gastroenterology, Maastricht University, Maastricht, The Netherlands. f.troost@intmed.unimaas.nl
authors.forename: Freddy J
authors.initials: FJ
authors.lastname: Troost
authors.forename: Robert-Jan M
authors.initials: RJ
authors.lastname: Brummer
authors.forename: Guido R M M
authors.initials: GR
authors.lastname: Haenen
authors.forename: Aalt
authors.initials: A
authors.lastname: Bast
authors.forename: Rachel I
authors.initials: RI
authors.lastname: van Haaften
authors.forename: Chris T
authors.initials: CT
authors.lastname: Evelo
authors.forename: Wim H M
authors.initials: WH
authors.lastname: Saris
mesh_terms: D000328:Adult; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism; D007501:Iron / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D015203:Reproducibility of Results; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
chemical_list: D015220:Calcium Channels; D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D017392:Thiobarbituric Acid Reactive Substances; D007501:Iron