Nuclear and cytoplasmic localization of interferon-tau in in vitro-produced bovine blastocysts.
Experiments were conducted to detect interferon-tau in bovine in vitro-derived blastocysts by transmission electron (TEM) and confocal microscopy. TEM showed the presence of IFN-tau in the cytoplasm and the nuclei of expanded blastocysts. Confocal microscopy similarly confirmed the presence of IFN-tau in the trophectoderm of blastocysts. The distribution of IFN-tau appeared variable with some cells showing strong labeling while others appeared to be devoid of the protein.
Johnson
Kelly M
KM
Division of Veterinary Medicine, Tulane National Primate Research Center, Covington, LA 70433, USA.
Alvarez
Xavier
X
Borkhsenious
Olga N
ON
Kubisch
H Michael
HM
D000818:Animals; D001755:Blastocyst / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D002417:Cattle / Q000196:embryology*; D046149:Embryo Culture Techniques / Q000662:veterinary; D005260:Female; D007370:Interferon Type I / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D018613:Microscopy, Confocal / Q000662:veterinary; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D011257:Pregnancy Proteins / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure
D007370:Interferon Type I; D011257:Pregnancy Proteins; C020947:interferon tau