Genetic variations and relationship among dengue virus type 3 strains isolated from patients with mild or severe form of dengue disease in Indonesia and Thailand.
Sequence analysis was conducted on structural and non-structural genes of 7 strains of dengue virus type-3 (DENV-3 virus) isolated in Indonesia and Thailand in the year 1973, 1994, and 1998 from patients with different clinical manifestations. In general, sequence similarity among isolates was greater than 93%, indicating that the mutation rate of DENV-3 circulating in this region was not more than 7% in the last 3 decades and suggesting that sequences that may responsible for viral architectures and/or biological function were strictly conserved. Mutations unique to viral strains associated with specific clinical manifestations were not found. Alignment of PrM/M and E nucleic acid sequences followed by parsimony analysis of sequences obtained in this study and published elsewhere allowed generation of phylogenetic trees, demonstrating that DENV-3 strains isolated in Indonesia in 1998 belonged to a separate cluster (subtype 2) from those isolated between 1973-1985 (subtype 1).
Raekiansyah
Muhareva
M
Department of Microbiology, Faculty of Medicine, University of Indonesia, Jakarta. tjahjanims@cbn.net.id
Pramesyanti
Andri
A
Bela
Budiman
B
Kosasih
Herman
H
Ma'roef
Chairin N
CN
Tobing
Sherley Yurike
SY
Rudiman
Panji I
PI
Alisjahbana
Bachti
B
Endi
Timothy P
TP
Green
Sharone
S
Kalayanarooj
Siripen
S
Rothman
Alan L
AL
Sudiro
T Mirawati
TM
D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018067:Flaviviridae / Q000472:pathogenicity; D005814:Genes, Viral; D014644:Genetic Variation; D006801:Humans; D007214:Indonesia; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013785:Thailand
D004279:DNA, Viral