Characterization of an aldehyde dehydrogenase from Euglena gracilis.
The free-living protist Euglena gracilis showed an enhanced growth when cultured in the dark with high concentrations of ethanol as carbon source. In a medium containing glutamate/malate plus 1% ethanol, E. gracilis reached a density of 3 x 10(7) cells/ml after 100 h of culture, which was 5 times higher than that attained with glutamate/malate or ethanol separately. This observation suggested the involvement of a highly active aldehyde dehydrogenase in the metabolism of ethanol. Purification of the E. gracilis aldehyde dehydrogenase from the mitochondrial fraction by affinity chromatography yielded an enrichment of 34 times and recovery of 33% of the total mitochondrial activity. SDS-PAGE and molecular exclusion chromatography revealed a native tetrameric protein of 160 kDa. Kinetic analysis showed Km values of 5 and 50 microM for propionaldehyde and NAD(+), respectively, and a Vm value of 1,300 nmol (min x mg protein)(-1). NAD(+) and NADH stimulated the esterase activity of the purified aldehyde dehydrogenase. The present data indicated that the E. gracilis aldehyde dehydrogenase has kinetic and structural properties similar to those of human aldehyde dehydrogenases class 1 and 2.
Rodríguez-Zavala
Jose S
JS
Departamento de Bioquímica. Instituto Nacional de Cardiología, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, México D.F. 14080, México. rodjos@cardiologia.org.mx
Ortiz-Cruz
Marco A
MA
Moreno-Sanchez
Rafael
R
D000079:Acetaldehyde / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D003470:Culture Media; D000431:Ethanol / Q000378:metabolism; D005056:Euglena gracilis / Q000201:enzymology* / Q000254:growth & development; D007700:Kinetics; D010084:Oxidation-Reduction
D003470:Culture Media; D000431:Ethanol; D000444:Aldehyde Dehydrogenase; D000079:Acetaldehyde