title: Potential of KM+ lectin in immunization against Leishmania amazonensis infection.
abstract: In the present study we evaluated Canavalia brasiliensis (ConBr), Pisum arvense (PAA) and Artocarpus integrifolia (KM+) lectins as immunostimulatory molecules in vaccination against Leishmania amazonensis infection. Although they induced IFN-gamma production, the combination of the lectins with SLA antigen did not lead to lesion reduction. However, parasite load was largely reduced in mice immunized with KM+ lectin and SLA. KM+ induced a smaller inflammatory reaction in the air pouch model and was able to inhibit differentiation of dendritic cells (BMDC), but to induce maturation by enhancing the expression of MHC II, CD80 and CD86. These observations indicate the modulatory role of plant lectins in leishmaniasis vaccination may be related to their action on the initial innate response.
authors.affiliation: Centro de Pesquisas Gonçalo Moniz-Fiocruz and Faculdade de Medicina da Bahia, Rua Waldemar Falcão 121, Salvador, BA 40295-001, Brazil.
authors.forename: Clarissa R
authors.initials: CR
authors.lastname: Teixeira
authors.forename: Karen A
authors.initials: KA
authors.lastname: Cavassani
authors.forename: Regis B
authors.initials: RB
authors.lastname: Gomes
authors.forename: Maria Jania
authors.initials: MJ
authors.lastname: Teixeira
authors.forename: Maria-Cristina
authors.initials: MC
authors.lastname: Roque-Barreira
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.forename: João Santana
authors.initials: JS
authors.lastname: da Silva
authors.forename: Aldina
authors.initials: A
authors.lastname: Barral
authors.forename: Manoel
authors.initials: M
authors.lastname: Barral-Netto
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000825:Animal Structures / Q000469:parasitology; D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D031622:Artocarpus; D032449:Canavalia; D003713:Dendritic Cells / Q000737:chemistry / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D007891:Leishmania / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology* / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D037121:Plant Lectins / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D000953:Antigens, Protozoan; D000949:Histocompatibility Antigens Class II; D037121:Plant Lectins; C039268:lectin, Canavalia; C036292:pea lectin; D018664:Interleukin-12; D007371:Interferon-gamma