Correction method for absorption-dependent signal enhancement by a liquid-core optical fiber.
The enhancement of a dissolved chemical's Raman scattering by a liquid-core optical fiber (LCOF) geometry is absorption dependent. This dependence leads to a disruption of the usual linear correlation between chemical concentration and Raman peak area. To recover the linearity, we augmented a standard LCOF Raman spectroscopy system with spectrophotometric capabilities, permitting sequential measurements of Raman and absorption spectra within the LCOF. Measurements of samples with identical Raman-scatterer concentrations but different absorption coefficients are described. Using the absorption values, we reduced variations in the measured Raman intensities from 60% to less than 1%. This correction method should be important for LCOF-based Raman spectroscopy of sample sets with variable absorption coefficients, such as urine and blood serum from multiple patients.
Qi
Dahu
D
University of Rochester, Rochester, New York 14627, USA. dahu@pas.rochester.edu
Berger
Andrew J
AJ
D000042:Absorption; D016477:Artifacts; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D055100:Optical Fibers; D010783:Photometry / Q000295:instrumentation* / Q000379:methods; D012996:Solutions; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods
D012996:Solutions