[Analysis of proteins in urinary tract stones and urine of urolithic patients].
In order to investigate the mechanism of urinary tract stone formation, we analyzed protein components in urine and the stone. Urinary proteins of healthy subjects and urolithic patients as well as protein components urinary tract stone of the urolithic patients were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Electrophoretic patterns of urinary proteins of the patients differed from those of healthy subjects after separating protein patterns into those larger than 66kDa or smaller than 30kDa. Protein constituents of urinary tract stone were mainly separated into 18 bands ranging from 26.8 to 143 kDa. Major bands among these 18 bands differed among stones from different patients. On western blotting, the developed intensities of Tamm-Horsfall protein (THP) were fainter than those of healthy subjects. Whereas intensities of albumin (ALB) were stronger than those of healthy subjects. Moreover, blotting patterns of THP of the patients on non-reducing SDS-PAGE were obviously broad. Thus, we suggest that analysis of fractionated urinary proteins or protein components of urinary tract stone may provide a tool for monitoring the prognosis or relapse in the patients.
Yokomizoi
Kayo
K
Analytical Laboratory Chemistry, Graduate School of Allied Health Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8519.
Nakayama
Aki
A
Hokazono
Eisaku
E
Ninomiya
Akiko
A
Miyake
Ruriko
R
Hiratsuka
Nobuo
N
Okuyama
Mitsuhiko
M
Kato
Yuji
Y
Kobayashi
Shizuko
S
Ito
Yoshihisa
Y
Shiba
Kiyoko
K
D000328:Adult; D000368:Aged; D000418:Albumins / Q000032:analysis; D000419:Albuminuria / Q000652:urine; D015415:Biomarkers / Q000652:urine; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009088:Mucoproteins / Q000032:analysis / Q000652:urine; D011506:Proteins / Q000032:analysis*; D011507:Proteinuria / Q000652:urine*; D012967:Sodium Dodecyl Sulfate; D014545:Urinary Calculi / Q000737:chemistry* / Q000175:diagnosis; D058949:Uromodulin
D000418:Albumins; D015415:Biomarkers; D009088:Mucoproteins; D011506:Proteins; C548612:UMOD protein, human; D058949:Uromodulin; D012967:Sodium Dodecyl Sulfate