title: Efficient and simple production of transgenic mice and rabbits using the new DMSO-sperm mediated exogenous DNA transfer method.
abstract: A high efficient and simple transgenic technology on mice and rabbits to transfect spermatozoa with exogenous DNA/DMSO complex to obtain transgenic offspring, which is namely called DMSO-sperm mediated gene transfer (SMGT). Mouse sperm could be either directly transfected via injection into testis or cultured in vitro with the plasmed DNA containing the enhanced green fluorescent protein (EGFP) that could be expressed in the embryos and offspring. Then, 36 living transgenic rabbits were produced using the same technology, and the transgenic ratio of 56.3% was detected using PCR and Southern blot. As the controls, the transgenic ratios of 39.6% and 47.8% have also been tested using the liposomes mediated technology of Tfx-50 Reagent or Lipefectamin-2000, respectively. The results show that the female transgenic rabbits, as the mammary gland bioreactor models, could express the human tissue plasminogen activator mutant (htPAm) in their mammary cells when they are adult.
authors.affiliation: Department of Life Science, Peking University, Beijing, China. shenwei427@yahoo.com.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.forename: Qingjie
authors.initials: Q
authors.lastname: Pan
authors.forename: Lingjiang
authors.initials: L
authors.lastname: Min
authors.forename: Huansheng
authors.initials: H
authors.lastname: Dong
authors.forename: Jixian
authors.initials: J
authors.lastname: Deng
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D005260:Female; D018014:Gene Transfer Techniques; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D010957:Plasmids / Q000737:chemistry*; D011817:Rabbits; D013094:Spermatozoa / Q000166:cytology
chemical_list: D004121:Dimethyl Sulfoxide