Complete 1H and 13C NMR assignment of digeneaside, a low-molecular-mass carbohydrate produced by red seaweeds.
Digeneaside (alpha-D-mannopyranosyl-(1-->2)-D-glycerate) was extracted from the red algae, Bostrychia binderii, and purified by adsorption and gel-filtration chromatography. HPLC and ESI-MS techniques were used to follow purification steps and characterize digeneaside. NMR spectroscopy experiments (1D 1H, 13C, DEPT and 2D HMQC, COSY and TOCSY) were used to fully assign the 1H and 13C spectra.
Ascêncio
Sérgio D
SD
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, CEP 81531-990, Curitiba, Paraná, Brazil.
Orsato
Alexandre
A
França
Robson A
RA
Duarte
M Eugênia R
ME
Noseda
Miguel D
MD
D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid; D005988:Glyceric Acids / Q000737:chemistry; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D008362:Mannosides / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012624:Seaweed / Q000737:chemistry* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
D002247:Carbon Isotopes; D005988:Glyceric Acids; D008362:Mannosides; C506886:digeneaside; D019344:Lactic Acid; C042971:glyceric acid; D008358:Mannose