title: Reconstruction of central carbon metabolism in Sulfolobus solfataricus using a two-dimensional gel electrophoresis map, stable isotope labelling and DNA microarray analysis.
abstract: In the last decade, an increasing number of sequenced archaeal genomes have become available, opening up the possibility for functional genomic analyses. Here, we reconstructed the central carbon metabolism in the hyperthermophilic crenarchaeon Sulfolobus solfataricus (glycolysis, gluconeogenesis and tricarboxylic acid cycle) on the basis of genomic, proteomic, transcriptomic and biochemical data. A 2-DE reference map of S. solfataricus grown on glucose, consisting of 325 unique ORFs in 255 protein spots, was created to facilitate this study. The map was then used for a differential expression study based on (15)N metabolic labelling (yeast extract + tryptone-grown cells (YT) vs. glucose-grown cells (G)). In addition, the expression ratio of the genes involved in carbon metabolism was studied using DNA microarrays. Surprisingly, only 3 and 14% of the genes and proteins, respectively, involved in central carbon metabolism showed a greater than two-fold change in expression level. All results are discussed in the light of the current understanding of central carbon metabolism in S. solfataricus and will help to obtain a system-wide understanding of this organism.
authors.affiliation: Biological and Environmental Systems Group, Department of Chemical and Process Engineering, University of Sheffield, Sheffield, UK.
authors.forename: Ambrosius P L
authors.initials: AP
authors.lastname: Snijders
authors.forename: Jasper
authors.initials: J
authors.lastname: Walther
authors.forename: Stefan
authors.initials: S
authors.lastname: Peter
authors.forename: Iris
authors.initials: I
authors.lastname: Kinnman
authors.forename: Marjon G J
authors.initials: MG
authors.lastname: de Vos
authors.forename: Harmen J G
authors.initials: HJ
authors.lastname: van de Werken
authors.forename: Stan J J
authors.initials: SJ
authors.lastname: Brouns
authors.forename: John
authors.initials: J
authors.lastname: van der Oost
authors.forename: Phillip C
authors.initials: PC
authors.lastname: Wright
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D007554:Isotopes / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D048229:Sulfolobus solfataricus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D007554:Isotopes; D002244:Carbon