title: Evaluation of serotypes of Streptococcus pneumoniae isolated from otitis media patients by multiplex polymerase chain reaction.
abstract: The increasing difficulty in the management of pneumococcal acute otitis media in parallel with increases in antimicrobial-resistant strains has led to much interest in pneumococcal capsular types for the adoption of effective prevention by vaccines. This study shows that multiplex polymerase chain reaction is a valuable and expeditious method for the capsular typing of pneumococci. The multiplex polymerase chain reaction method accurately detects the majority of serotypes and serogroups frequently isolated from pediatric patients with acute otitis media, allowing the characterization of the colonization patterns for further implications of pneumococcal vaccines.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan.
authors.forename: Dewan S
authors.initials: DS
authors.lastname: Billal
authors.forename: Muneki
authors.initials: M
authors.lastname: Hotomi
authors.forename: Sayeeda
authors.initials: S
authors.lastname: Tasnim
authors.forename: Keiji
authors.initials: K
authors.lastname: Fujihara
authors.forename: Noboru
authors.initials: N
authors.lastname: Yamanaka
mesh_terms: D016667:Bacterial Capsules; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D009305:Nasopharynx / Q000382:microbiology; D010033:Otitis Media / Q000382:microbiology*; D011008:Pneumococcal Infections / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000302:isolation & purification