HPLC determination of polydatin in rat biological matrices: application to pharmacokinetic studies.
A reversed-phase high-performance liquid chromatographic (RPHPLC) method has been developed for the determination of polydatin (PD) in rat plasma, bile, urine, feces and tissue homogenates using 2,3,5,4'-tetrahydroxychrysophenine-beta-d-glucoside as an internal standard. The sample pretreatment included deproteinization for plasma samples and a liquid-liquid extraction for bile, urine, feces and tissue homogenates. Separation was obtained on a C18 reversed-phase column with the mobile phase consisting of methanol and water (35:65 v/v). The flow rate was 1 ml/min and the effluent was monitored at 310 nm. The method showed good linearity over the concentration ranges employed for various matrices (r > 0.998). The quantification limits of PD in rat plasma, bile, urine, feces and tissue homogenates were 0.0251, 0.126, 0.025 microg/ml, 0.189 and 0.0378 microg/g, respectively. The accuracy and precision of the method were less than 12.0% for the various matrices. No interferences from endogenous substances were found. The method was successfully applied to study the pharmacokinetics of PD in rats after intravenous administration.
Gao
Shouhong
S
School of Pharmacy, Second Military Medical University, Shanghai Key Laboratory for Pharmaceutical Metabolites Research, No. 325, Guohe Road, Shanghai 200433, PR China.
Fan
Guorong
G
Hong
Zhanying
Z
Yin
Xueping
X
Yang
Shaolin
S
Wu
Yutian
Y
D000818:Animals; D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry* / Q000493:pharmacokinetics*; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013267:Stilbenes / Q000032:analysis* / Q000737:chemistry* / Q000493:pharmacokinetics*; D013678:Technology, Pharmaceutical / Q000379:methods*; D014466:Ultraviolet Rays
D005960:Glucosides; D013267:Stilbenes; C058229:polydatin; D000432:Methanol