An in vitro study of the protective effect of the flavonoid silydianin against reactive oxygen species.
The inhibitory effect of silydianin, an active constituent of Silybium marianum, on the in vitro production and release of oxidative products has been examined. Polymorphonuclear neutrophils (PMNs) play a primary role in the initiation and propagation of inflammatory responses. Their apoptosis is a major mechanism associated with the resolution of inflammatory reactions. Neutrophils were assessed for caspase-3 activity, the rst step in the execution phase of apoptosis. When cells were cultured with 100 microM silydianin for 24 h, caspase-3 was activated. Induction of apoptosis by silydianin was accompanied by a decrease in luminol-enhanced chemiluminescence as well as superoxide radical (O2*-) release in freshly isolated cells and lipid peroxidation in mouse spleen microsomes. No significant effect of silydianin on PMN hydrogen peroxide production evaluated by a flow cytometric dichlorofluorescin oxidation assay was found. Such results indicate a possible antiinflammatory activity for silydianin, which regulates caspase-3 activation, affects cell membranes and acts as a free radical scavenger.
Zielińska-Przyjemska
Małgorzata
M
Department of Pharmaceutical Biochemistry, Poznań University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland. mzielin@am.poznan.pl
Wiktorowicz
Krzysztof
K
D000328:Adult; D000818:Animals; D053148:Caspase 3; D061945:Caspase Inhibitors; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D051379:Mice; D008861:Microsomes / Q000187:drug effects; D020944:Silybum marianum / Q000737:chemistry*; D015394:Molecular Structure; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors*; D012838:Silymarin / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology
D061945:Caspase Inhibitors; D017382:Reactive Oxygen Species; D012838:Silymarin; C015505:silidianin; C505407:CASP3 protein, human; C505409:Casp3 protein, mouse; D053148:Caspase 3