Serosurveillance of acute Mycoplasma pneumoniae infection among HIV infected patients with pulmonary complaints in Chennai, Southern India.
BACKGROUND
The true seroepidemiology of acute Mycoplasma pneumoniae infection in HIV infected individuals is ambiguous.


METHODS
This study examined the serosurveillance of IgM antibodies to M. pneumoniae in HIV infected patients presenting with pulmonary symptoms at a tertiary AIDS care center in Chennai, Southern India, using cold-haemagglutination test and commercial enzyme linked immunosorbent assay in acute serum specimens.


RESULTS
One hundred HIV infected patients had enrolled in the study; 21 (21%) were positive for M. pneumoniae IgM antibodies by ELISA and 34 (34%) showed evidence of cold hemagglutinins.


CONCLUSIONS
This serosurveillance study reports a 21% prevalence of M. pneumoniae IgM antibody among HIV infected patients with pulmonary symptoms by ELISA and non-specific diagnosis was confirmed in 34% of the cases screened. Determination of cold agglutination titer could be used as a substitute to other expensive procedures in limited resource settings and third-world nations to diagnose M. pneumoniae infections for prompt initiation of therapy, as CAT has been found to be 100% sensitive and 84% specific in the diagnosis of M. pneumoniae infection.
Shankar
Esaki Muthu
EM
Mycoplasma Laboratory of the Department of Microbiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, Tamil Nadu, India.
Kumarasamy
Nagalingeswaran
N
Balakrishnan
Pachamuthu
P
Solomon
Suniti
S
Lejith
Ravi
R
Vengatesan
A
A
Anand Rao
Usha
U
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis* / Q000453:epidemiology*; D000208:Acute Disease; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015658:HIV Infections / Q000382:microbiology; D006386:Hemagglutination Tests; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007194:India / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000453:epidemiology*; D016036:Seroepidemiologic Studies
D000907:Antibodies, Bacterial; D007075:Immunoglobulin M