title: Mismatch tolerance by DNA polymerase Pol4 in the course of nonhomologous end joining in Saccharomyces cerevisiae.
abstract: In yeast, the nonhomologous end joining pathway (NHEJ) mobilizes the DNA polymerase Pol4 to repair DNA double-strand breaks when gap filling is required prior to ligation. Using telomere-telomere fusions caused by loss of the telomeric protein Rap1 and double-strand break repair on transformed DNA as assays for NHEJ between fully uncohesive ends, we show that Pol4 is able to extend a 3'-end whose last bases are mismatched, i.e., mispaired or unpaired, to the template strand.
authors.affiliation: Laboratoire de Radiobiologie de l'ADN, Service de Radiobiologie Moléculaire et Cellulaire, UMR217 du CNRS, CEA/Fontenay, Fontenay-aux-Roses, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Pardo
authors.forename: Emilie
authors.initials: E
authors.lastname: Ma
authors.forename: Stéphane
authors.initials: S
authors.lastname: Marcand
mesh_terms: D000483:Alleles; D020137:Base Pair Mismatch; D001483:Base Sequence; D019951:DNA Polymerase beta; D017931:DNA Primers; D004260:DNA Repair; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000502:physiology*; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D017931:DNA Primers; C440829:RAP1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins; D014157:Transcription Factors; D019951:DNA Polymerase beta; D004259:DNA-Directed DNA Polymerase; C084892:POL4 protein, S cerevisiae