[Experimental study of the effect of Bushen Huoxue recipe on biological characters of osteoblasts in vitro].
OBJECTIVE
To observe the effect of Bushen Huoxue Recipe on cell proliferation, differentiation, maturation and mineralization in rats cultured osteoblasts.


METHODS
Osteoblasts from craniums of newly born SD rats were cultured in vitro. MTT, PNPP and ARS were used to observe the proliferation, activity of ALP and the number of mineral node of cultured osteoblasts in vitro.


RESULTS
It was found that Bushen Huoxue Recipe had the effect on stimulating cell proliferation, activity of ALP and the number of mineral node of cultured osteoblasts (P < 0.01 or P < 0.05).


CONCLUSIONS
Bushen Huoxue Recipe can promote proliferation, differentiation, maturation and mineralization of the osteoblasts cultured in vitro.
Zang
Hongmin
H
Department of Orthopedics, Second Hospital of Xi' an Jiaotong University, Xi' an 710004.
Chen
Junchang
J
Liu
Yiheng
Y
Liu
Runxia
R
Wang
Kunzheng
K
D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000166:cytology
D004365:Drugs, Chinese Herbal; D000469:Alkaline Phosphatase