The structure of the carboxyltransferase component of acetyl-coA carboxylase reveals a zinc-binding motif unique to the bacterial enzyme.
Acetyl-coA carboxylase (ACC) is a central metabolic enzyme that catalyzes the committed step in fatty acid biosynthesis: biotin-dependent conversion of acetyl-coA to malonyl-coA. The bacterial carboxyltransferase (CT) subunit of ACC is a target for the design of novel therapeutics that combat severe, hospital-acquired infections resistant to the established classes of frontline antimicrobials. Here, we present the structures of the bacterial CT subunits from two prevalent nosocomial pathogens, Staphylococcus aureus and Escherichia coli, at a resolution of 2.0 and 3.0 A, respectively. Both structures reveal a small, independent zinc-binding domain that lacks a complement in the primary sequence or structure of the eukaryotic homologue.
Bilder
Patrick
P
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
Lightle
Sandra
S
Bainbridge
Graeme
G
Ohren
Jeffrey
J
Finzel
Barry
B
Sun
Fang
F
Holley
Susan
S
Al-Kassim
Loola
L
Spessard
Cindy
C
Melnick
Michael
M
Newcomer
Marcia
M
Waldrop
Grover L
GL
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D001665:Binding Sites; D019878:Carboxyl and Carbamoyl Transferases / Q000378:metabolism*; D003428:Cross Infection / Q000201:enzymology; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology; D005057:Eukaryotic Cells / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D013211:Staphylococcus aureus / Q000201:enzymology; D015032:Zinc / Q000378:metabolism*
D019878:Carboxyl and Carbamoyl Transferases; D000103:Acetyl-CoA Carboxylase; D015032:Zinc