Metalloantibiotic Mn(II)-bacitracin complex mimicking manganese superoxide dismutase.
Superoxide dismutase (SOD) activities of various metallobacitracin complexes were evaluated using the riboflavin-methionine-nitro blue tetrazolium assay. The radical scavenging activity of various metallobacitracin complexes was shown to be higher than those of the negative controls, e.g., free transition metal ions and metal-free bacitracin. The SOD activity of the complex was found to be in the order of Mn(II)>Cu(II)>Co(II)>Ni(II). Furthermore, the effect of bacitracin and their complexation to metals on various microorganisms was assessed by antibiotic susceptibility testing. Moreover, molecular modeling and quantum chemical calculation of the metallobacitracin complex was performed to evaluate the correlation of electrostatic charge of transition metal ions on the SOD activity.
Piacham
Theeraphon
T
Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.
Isarankura-Na-Ayudhya
Chartchalerm
C
Nantasenamat
Chanin
C
Yainoy
Sakda
S
Ye
Lei
L
Bülow
Leif
L
Prachayasittikul
Virapong
V
D000818:Animals; D001414:Bacitracin / Q000031:analogs & derivatives*; D040761:Biomimetic Materials / Q000494:pharmacology*; D002413:Cations, Divalent / Q000737:chemistry; D002417:Cattle; D003035:Cobalt / Q000494:pharmacology; D003300:Copper / Q000737:chemistry; D004492:Edetic Acid / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D009532:Nickel / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism*
D002413:Cations, Divalent; D001414:Bacitracin; D012710:Serum Albumin, Bovine; D003035:Cobalt; D008345:Manganese; D003300:Copper; D009532:Nickel; D004492:Edetic Acid; D013482:Superoxide Dismutase