title: Fundamental study of expression-library immunization against Taenia taeniaeformis in rats.
abstract: Expression-library immunization has been proposed as an effective means to screen a large number of genes of the pathogen as candidate protective molecules. In this study, we examined the efficacy of expression-library immunization using a T. taeniaeformis rat model system. Total RNAs were isolated from the last 15 segments of adult T. taeniaeformis and poly A RNA was purified. cDNA library was produced using SuperScript Plasmid System, which contains a mammalian expression vector, pCMV*SPORT6. From about 3,500 clones examined, more than 800 clones were found to contain DNA fragments. About 200 clones were sequenced and the homology search was carried out. The blast search revealed that 29% of the expression genes were mitochondrial genes (rRNA; 17%, protein; 12%). Nuclear rRNA genes (10%), nuclear protein (9%) and genes from Escherichia coli were also detected. Forty-two percent of sequences did not show a significant similarity to any genes deposited in the public database. Rats were immunized with expression-library and injected orally with 1,000 T. taeniaeformis eggs. However the protective effect of expression-library vaccine was not confirmed.
authors.affiliation: Department of Laboratory Animal Science, School of Veterinary Medicaine, Faculty of Agriculture, Totori University, Japan. mokamoto@muses.tottori-u.ac.jp
authors.forename: Munehiro
authors.initials: M
authors.lastname: Okamoto
authors.forename: Kanako
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Akiko
authors.initials: A
authors.lastname: Shibui
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shibahara
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth; D005260:Female; D015870:Gene Expression; D007114:Immunization; D015138:RNA, Nuclear; D051381:Rats; D013621:Taenia / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D013622:Taeniasis / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D019444:Vaccines, DNA / Q000494:pharmacology
chemical_list: D000909:Antibodies, Helminth; D015138:RNA, Nuclear; D019444:Vaccines, DNA