title: Affinity capillary electrophoresis with a DNA-nanoparticle conjugate as a new tool for genotyping.
abstract: We have developed a novel method for genotyping based on free solution affinity capillary electrophoresis. We prepared DNA-nanoparticle conjugates by mixing biotin-modified DNA and NeutrAvidin-modified polystyrene nanoparticles; this mixture was then injected into a capillary. Subsequently, we injected the fluorescent-labeled sample DNAs into the capillary, applied the voltage, increased its temperature after 7 min, and detected the fluorescence at its anodic end. This novel method was applied for genotyping human c-K-ras, and the three genotypes were definitely distinguishable with high reproducibility. This method can be easily automated, and it is useful for high-throughput gene mutation analysis.
authors.affiliation: Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Adachi
authors.forename: Naohiro
authors.initials: N
authors.lastname: Noda
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakashige
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsuneda
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kanagawa
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes; D011905:Genes, ras; D005838:Genotype; D036103:Nanotechnology
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D004247:DNA