[Changes in spleen dendritic cells in multiple organ dysfunction syndrome in mice].
OBJECTIVE
To explore the pathological features of the splenic dendritic cells (DCs) and their role in the pathogenesis of multiple organ dysfunction syndrome (MODS).


METHODS
The MODS model of mice was reproduced. The changes in DCs in the spleen in mice with MODS were studied using light microscope, electronic microscope, immunohistochemistry (CD205, CD86) and transferase mediated dUTP-biotin nick end labeling (TUNEL).


RESULTS
In the early stage (3 hours), there was hyperplasia with increased activity of DCs in the spleen, accompanied by apoptosis of a large number of lymphocytes. CD4(+)/CD8(+) ratio declined in the peripheral blood. In the MODS stage (10-12 days), the number of DCs increased but with declined function. CD4(+)/CD8(+) ratio declined markedly in peripheral blood.


CONCLUSIONS
During the course of MODS, changes in the number and activity of DCs in spleen are very important in the establishment of immune response and maintenance of immune function.
Lu
Jiang-yang
JY
Department of Pathology, First Affiliated Hospital of General Hospital of PLA, Beijing 100037, China. lujy@263.net
Li
Zhi-hong
ZH
Wang
Xiao-hong
XH
Yang
Yi
Y
Li
Ling
L
D000818:Animals; D016516:CD4-CD8 Ratio; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009102:Multiple Organ Failure / Q000276:immunology / Q000473:pathology*; D011897:Random Allocation; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000473:pathology*