title: Antibody response to four secretory proteins from Mycobacterium tuberculosis and their complex antigen in TB patients.
abstract: OBJECTIVE
  To evaluate antibody responses to a panel of four protein antigens secreted from Mycobacterium tuberculosis, CFP-21, ESAT-6, MPT-63 and MPT-64, and their complex antigen.
  
  
  METHODS
  Seventy-eight samples of serum and cerebral spinal fluid were tested by ELISA.
  
  
  RESULTS
  Serum IgM antibody positive rates of protein antigens CFP-21, ESAT-6, MPT-63 and MPT-64 and their multi-antigen complex were respectively 34.6%, 60.3%, 52.6%, 78.2% and 96.2%; IgG antibody positive rates were respectively 46.2%, 64.1%, 93.6%, 57.7% and 84.6%. The specificity of the antibodies to the four proteins secreted from M. tuberculosis and its mixture antigen was 100%; sensitivity to the multi-antigen complex was higher than for the four single antigens. Among the IgM positive rates, the differences between the multi-antigen complex and CFP-21, ESAT-6, MPT-63 and MPT-64 were significant (P < 0.01); among the IgG positive rates, the differences between the multi-antigen complex and CFP-21, ESAT-6 and MPT-64 were significant (P < 0.01). Although the IgG positive rate for MPT-63 was higher than for the multi-antigen complex, the difference was not significantly different (P > 0.05).
  
  
  CONCLUSIONS
  These findings indicate that the multi-antigen complex composed of CFP-21, ESAT-6, MPT-63 and MPT-64 may be useful in the diagnosis of TB.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai, China. blwang@fudan.edu.cn
authors.forename: B L
authors.initials: BL
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: Z M
authors.initials: ZM
authors.lastname: Li
authors.forename: Y M
authors.initials: YM
authors.lastname: Xu
authors.forename: X H
authors.initials: XH
authors.lastname: Weng
authors.forename: H H
authors.initials: HH
authors.lastname: Wang
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000097:blood / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C113719:CFP21 protein, Mycobacterium tuberculosis; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C080865:MPB64 protein, Mycobacterium; C103339:MPT63 antigen, Mycobacterium tuberculosis