Alleviation of free radical mediated oxidative and genotoxic effects of cadmium by farnesol in Swiss albino mice.
Farnesol is an isoprenoid found in essential oils of ambrette seeds, citronella and in various aromatic plants. Exposure to cadmium from various sources affects the renal system adversely and Cd is an established genotoxic agent. In the present study, we evaluated the antigenotoxic and antioxidant efficacy of farnesol against cadmium chloride (CdCl2)-induced renal oxidative stress and genotoxicity in Swiss albino mice. Single, intraperitoneal doses of CdCl2(5 mg/kg body weight) for 24 h resulted in a significant (P < 0.001) increase in chromosomal aberration and micronuclei formation. The oral administration of farnesol at two doses (1% and 2% per kg body weight) for seven consecutive days showed significant (P < 0.05) suppression of the genotoxic effects of CdCl2 in the modulator groups. To study the mechanism by which farnesol exerts its antigenotoxic potential, enzymes involved in metabolism and detoxification were estimated. CdCl2 intoxication adversely affected the renal antioxidant armory and increased TBARS formation and xanthine oxidase levels significantly (P < 0.001). Farnesol showed a significant (P < 0.001) recovery in antioxidant status viz, GSH content (and its dependent enzymes) and catalase activity. Farnesol pretreatment in CdCl2-intoxicated mice showed marked (P < 0.001) suppression of TBARS' formation and XO activity. Our results support the conclusion that the anticlastogenic effect of farnesol could be due to restoration of antioxidants and inhibition of oxidative damage.
Jahangir
Tamanna
T
Section of Chemoprevention and Nutrition Toxicology, Department of Medical Elementology and Toxicology, Jamia Hamdard, Hamdard University, Hamdard Nagar, New Delhi, India.
Khan
Tajdar Husain
TH
Prasad
Lakshmi
L
Sultana
Sarwat
S
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D019256:Cadmium Chloride / Q000494:pharmacology*; D002869:Chromosome Aberrations; D005204:Farnesol / Q000494:pharmacology*; D005609:Free Radicals; D007668:Kidney / Q000187:drug effects; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens; D018384:Oxidative Stress; D017392:Thiobarbituric Acid Reactive Substances; D014969:Xanthine Oxidase / Q000378:metabolism
D000975:Antioxidants; D005609:Free Radicals; D009153:Mutagens; D017392:Thiobarbituric Acid Reactive Substances; D005204:Farnesol; D014969:Xanthine Oxidase; D019256:Cadmium Chloride