title: [An analysis for the phenotype and genotype of autosomal dominant polycystic kidney disease from two Chinese families].
abstract: OBJECTIVE
  To scan for mutations of polycystic kidney disease 1 gene (PKD1) in Chinese population in order to find some features about Chinese patients and a better approach to detect mutations.
  
  
  METHODS
  Twenty-five PKD-affected individuals from twenty-one unrelated genealogies and sixteen controls participated in the study. Thirty-five blood samples and six tissues were obtained after receiving informed consent and were in accordance with institutional ethical guidelines. Genomic DNA was isolated from peripheral blood using standard procedures. PCR amplification of genomic DNA was performed to generate the aimed fragments. Amplified fragments were analyzed by denaturing gradient gel electrophoresis (DGGE). A GC clamp was attached to the 5' primer. After that, the abnormal fragments were sequenced on freshly amplified specific PCR products with the dideoxynucleotide chain termination method. Sequencing was performed for all samples to evaluate DGGE.
  
  
  RESULTS
  Aimed fragments of exons 44 and 45 were amplified. DGGE detected eleven abnormal PCR fragments. Two novel mutations were identified by sequencing, included one nonsense mutation (C12217T) and one frameshift (12431delCT). In addition, one polymorphism (A50747C) was identified. The mutation detection rate is 8% in our study.
  
  
  CONCLUSIONS
  Two novel pathogenic mutations were detected, including one nonsense mutation (C12217T) and one frameshift (12431delCT).
authors.affiliation: Department of Urology Surgery, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: De-xuan
authors.initials: DX
authors.lastname: Gao
authors.forename: Qing-wei
authors.initials: QW
authors.lastname: Cao
authors.forename: Ke-jia
authors.initials: KJ
authors.lastname: Ding
authors.forename: Yue-ran
authors.initials: YR
authors.lastname: Zhao
authors.forename: Lai-cheng
authors.initials: LC
authors.lastname: Wang
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Niu
authors.forename: Jia-ju
authors.initials: JJ
authors.lastname: Lu
mesh_terms: D044466:Asian People / Q000235:genetics; D018389:Codon, Nonsense; D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D005192:Family Health; D005260:Female; D016368:Frameshift Mutation; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010375:Pedigree; D010641:Phenotype; D016891:Polycystic Kidney, Autosomal Dominant / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011506:Proteins / Q000235:genetics*; D050396:TRPP Cation Channels
chemical_list: D018389:Codon, Nonsense; D011506:Proteins; D050396:TRPP Cation Channels