Cry1Ab protein from Bt transgenic rice does not residue in rhizosphere soil.
Expression of Cry1Ab protein in Bt transgenic rice (KMD) and its residue in the rhizosphere soil during the whole growth in field, as well as degradation of the protein from KMD straw in five soils under laboratory incubation were studied. The residue of Cry1Ab protein in KMD rhizosphere soil was undetectable (below the limit of 0.5 ng/g air-dried soil). The Cry1Ab protein contents in the shoot and root of KMD were 3.23-8.22 and 0.68-0.89 microg/g (fresh weight), respectively. The half-lives of the Cry1Ab protein in the soils amended with KMD straw (4%, w/w) ranged from 11.5 to 34.3d. The residence time of the protein varied significantly in a Fluvio-marine yellow loamy soil amended with KMD straw at the rate of 3, 4 and 7%, with half-lives of 9.9, 13.8 and 18d, respectively. In addition, an extraction method for Cry1Ab protein in soil was developed, with extraction efficiencies of 46.4-82.3%.
Wang
Haiyan
H
Institute of Nuclear Agricultural Sciences, Zhejiang University, Hua-jia-chi Campus, Hangzhou 310029, China.
Ye
Qingfu
Q
Wang
Wei
W
Wu
Licheng
L
Wu
Weixiang
W
D000083722:Bacillus thuringiensis Toxins; D001419:Bacteria / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D001427:Bacterial Toxins / Q000032:analysis* / Q000235:genetics; D004731:Endotoxins / Q000032:analysis* / Q000235:genetics; D004784:Environmental Monitoring / Q000379:methods; D004787:Environmental Pollution; D006460:Hemolysin Proteins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D010572:Pest Control, Biological; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D012987:Soil; C030956:insecticidal crystal protein, Bacillus Thuringiensis