title: Enhanced mucosal immunogenicity of prion protein following fusion with B subunit of Escherichia coli heat-labile enterotoxin.
abstract: Mucosal vaccine against prion protein (PrP), a major component of prions, is urgently awaited since the oral transmission of prions from cattle to humans is highly suspected. In the present study, we produced recombinant bovine and mouse PrPs fused with or without the B subunit of Escherichia coli heat-labile enterotoxin (LTB) and intranasally immunized mice with these fused proteins. Fusion with LTB markedly enhanced the mucosal immunogenicity of bovine PrP, producing a marked increase in specific IgG and IgA titer in serum. Mouse PrP also showed slightly increased immunogenicity following fusion with LTB. These results demonstrate that LTB-fused PrPs might be potential candidates for protective mucosal prion vaccines.
authors.affiliation: PRESTO Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama, Japan.
authors.forename: Hitoki
authors.initials: H
authors.lastname: Yamanaka
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ishibashi
authors.forename: Naohiro
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yoshikawa
authors.forename: Risa
authors.initials: R
authors.lastname: Nakamura
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Okimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Arakawa
authors.forename: Takao
authors.initials: T
authors.lastname: Tsuji
authors.forename: Shigeru
authors.initials: S
authors.lastname: Katamine
authors.forename: Suehiro
authors.initials: S
authors.lastname: Sakaguchi
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000906:Antibodies / Q000097:blood; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology*; D005260:Female; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011328:Prions / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000906:Antibodies; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011328:Prions; D011993:Recombinant Fusion Proteins; C027538:heat-labile enterotoxin, E coli