GD3-replica peptides selected from a phage peptide library induce a GD3 ganglioside antibody response.
GD3-replica peptides were obtained from a phage peptide library and an anti-GD3 monoclonal antibody (Mab) (4F6), and anti-GD3 Mabs were generated by immunizing a peptide GD3P4. A Mab, 3D2 was found to recognize GD3 by immunohistochemical approaches. Amino acid analysis of heavy and light chain variable regions of 4F6 and 3D2 showed that the respective chains had the same length, and only a few different amino acid substitutions were found. The present data indicate that the immunogenic GD3P4 is processed in a certain size and exposed on the antigen-presenting cells with a molecular shape quite similar to that of the GD3 epitope in 4F6.
Popa
Iuliana
I
INSERM U.346, Edouard Herriot Hospital, Lyon, France.
Ishikawa
Dai
D
Tanaka
Michinori
M
Ogino
Koichi
K
Portoukalian
Jacques
J
Taki
Takao
T
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry*; D000917:Antibody Formation; D017951:Antigen Presentation; D001483:Base Sequence; D005732:Gangliosides / Q000276:immunology*; D007114:Immunization; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D051379:Mice; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000276:immunology*
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D005732:Gangliosides; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D010455:Peptides; C026226:ganglioside, GD3