Nuclear envelope localization of human UNC84A does not require nuclear lamins.
The SUN proteins are a conserved family of proteins in eukaryotes. Human UNC84A (Sun1) is a homolog of Caenorhabditis elegans UNC-84, a protein involved in nuclear anchorage and migration. We have analyzed targeting of UNC84A to the nuclear envelope (NE) and show that the N-terminal 300 amino acids are crucial for efficient NE localization of UNC84A whereas the conserved C-terminal SUN domain is not required. Furthermore, we demonstrate by combining RNA interference with immunofluorescence and fluorescence recovery after photobleaching analysis that localization and anchoring of UNC84A is not dependent on the lamin proteins, in contrast to what had been observed for C. elegans UNC-84.
Hasan
Sameez
S
Institute of Biochemistry, ETH Zurich, Schafmattstrasse 18, CH-8093 Zürich, Switzerland.
Güttinger
Stephan
S
Mühlhäusser
Petra
P
Anderegg
Fabian
F
Bürgler
Simone
S
Kutay
Ulrike
U
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D034882:Lamins / Q000502:physiology*; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009685:Nuclear Envelope / Q000737:chemistry / Q000378:metabolism*; D034881:Nuclear Lamina / Q000502:physiology; D009687:Nuclear Proteins; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000494:pharmacology; D014162:Transfection
D002352:Carrier Proteins; D034882:Lamins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; C114799:SPAG4 protein, human; C506117:SUN1 protein, human