title: Outer membrane vesicles from group B Neisseria meningitidis delta gna33 mutant: proteomic and immunological comparison with detergent-derived outer membrane vesicles.
abstract: We compared the proteome of detergent-derived group B Neisseria meningitidis (MenB) outer membrane vesicles (DOMVs) with the proteome of outer membrane vesicles (m-OMVs) spontaneously released into culture supernatant by MenB delta gna33, a mutant in which the gene coding for a lytic transglycosylase homologous to the E. coli MltA was deleted. In total, 138 proteins were identified in DOMVs by 1- and 2-DE coupled with MS; 64% of these proteins belonged to the inner membrane and cytoplasmic compartments. By contrast, most of the 60 proteins of m-OMVs were classified by PSORT as outer membrane proteins. When tested for their capacity to elicit bactericidal antibodies, m-OMVs elicited a broad protective activity against a large panel of MenB strains. Therefore, the identification of mutations capable of conferring an OMV-releasing phenotype in bacteria may represent an attractive approach to study bacterial membrane composition and organization, and to design new efficacious vaccine formulations.
authors.affiliation: Biochemistry and Molecular Biology Unit, Chiron Vaccines, Siena, Italy.
authors.forename: Germano
authors.initials: G
authors.lastname: Ferrari
authors.forename: Ignazio
authors.initials: I
authors.lastname: Garaguso
authors.forename: Jeannette
authors.initials: J
authors.lastname: Adu-Bobie
authors.forename: Francesco
authors.initials: F
authors.lastname: Doro
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Taddei
authors.forename: Alessia
authors.initials: A
authors.lastname: Biolchi
authors.forename: Brunella
authors.initials: B
authors.lastname: Brunelli
authors.forename: Marzia Monica
authors.initials: MM
authors.lastname: Giuliani
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Pizza
authors.forename: Nathalie
authors.initials: N
authors.lastname: Norais
authors.forename: Guido
authors.initials: G
authors.lastname: Grandi
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure; D002850:Chromatography, Gel; D019295:Computational Biology; D003902:Detergents / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005434:Flow Cytometry; D017353:Gene Deletion; D013058:Mass Spectrometry; D038541:Neisseria meningitidis, Serogroup B / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D012703:Serotyping
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003902:Detergents