title: HRPT2 gene alterations in ossifying fibroma of the jaws.
abstract: Ossifying fibroma (OF) is a benign neoplasm related to bone characterized by a progressive enlargement of the affected jaw. Recently, the candidate tumor suppressor gene HRPT2 was identified and alterations in this gene were related with the Hyperparathyroidism-jaw tumor syndrome that is characterized by parathyroid adenoma or carcinoma, fibro-osseous lesions (mainly OF) of the jaws, and renal lesions. The purpose of the present study was to evaluate the HRPT2 gene in OF. Tumour and blood samples were obtained from 3 patients with OF and one with juvenile ossifying fibroma (JOF). The results demonstrated three novel mutations in two out of three genotyped OF's. Interestingly, one of these patients showed a germ-line mutation after blood analysis. RT-PCR amplification was performed to analyze HRPT2 mRNA expression and only wild-type HRPT2 transcript was found in all tumours. Investigation of the parafibromin protein by immunohistochemistry showed a similar pattern of immunolocalization with strong nuclear and cytoplasmic staining in all cases. In conclusion, the present study shows for the first time mutations of HRPT2 gene in OF and suggests that OF may arise due to haploinsufficiency of the HRPT2 gene.
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Flávio Juliano
authors.initials: FJ
authors.lastname: Pimenta
authors.forename: Letícia Ferreira
authors.initials: LF
authors.lastname: Gontijo Silveira
authors.forename: Gabriela Cordeiro
authors.initials: GC
authors.lastname: Tavares
authors.forename: Andreza Campos
authors.initials: AC
authors.lastname: Silva
authors.forename: Paolla Freitas
authors.initials: PF
authors.lastname: Perdigão
authors.forename: Wagner Henriques
authors.initials: WH
authors.lastname: Castro
authors.forename: Marcus Vinícius
authors.initials: MV
authors.lastname: Gomez
authors.forename: Bin Tean
authors.initials: BT
authors.lastname: Teh
authors.forename: Luiz
authors.initials: L
authors.lastname: De Marco
authors.forename: Ricardo Santiago
authors.initials: RS
authors.lastname: Gomez
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D018214:Fibroma, Ossifying / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D008339:Mandibular Neoplasms / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C454900:CDC73 protein, human; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D025521:Tumor Suppressor Proteins