Expression of oxidative stress-related molecules in circulating leukocytes and urine in patients with chronic viral hepatitis.
OBJECTIVE
Oxidative stress plays a role in pathogenesis of chronic viral hepatitis. Expression of oxidative stress-related molecules remains to be clarified.


METHODS
4-hydroxy-2-nonenal (4-HNE), 4-hydroxy-2-hexenal (4-HHE), catalase, superoxide dismutase-1 (SOD-1), glutathione peroxidase-1, thioredoxin (TRX) in leukocytes and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) were examined in 164 persons, including 130 chronic viral hepatitis patients and 34 normal individuals, by Western blot analysis and enzyme-linked immunosorbent assay, respectively. Hepatic expression of these proteins was immunohistochemically examined in 12 patients with chronic viral hepatitis, compared with three persons without liver damage.


RESULTS
The 4-HNE/beta-actin ratios in chronic viral hepatitis were significantly higher than those in normal individuals (P<0.01), and were significantly correlated with asparate aminotransferase (AST) and alanine aminotransferase (ALT) (P<0.01, each). The catalase/beta-actin and SOD-1/beta-actin ratios in chronic viral hepatitis were higher than those in normal individuals, and were significantly correlated with 4-HNE/beta-actin ratios (P<0.01, each). Hepatic expression of 4-HNE, 4-HHE, catalase, SOD-1 and TRX in chronic viral hepatitis was higher than that without liver damage. Urinary excretion of 8-OHdG was not changed in chronic viral hepatitis.


CONCLUSIONS
The results of the present study suggest that expression of oxidative stress-related molecules in leukocytes is upregulated in relation to serum aminotransferase levels.
Saeki
Toshiya
T
Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, Yonago, Japan.
Ichiba
Miho
M
Tanabe
Naotada
N
Ueki
Masaru
M
Okamoto
Kinya
K
Matsunaga
Yoshiko
Y
Hosho
Keiko
K
Kanbe
Takamasa
T
Tsuchiya
Hiroyuki
H
Kurimasa
Akihiro
A
Yamada
Sadako
S
Hirooka
Yasuaki
Y
Hisatome
Ichiro
I
Kishimoto
Yukihiro
Y
Suou
Takeaki
T
Murawaki
Yoshikazu
Y
Kawasaki
Hironaka
H
Yodoi
Junji
J
Shiota
Goshi
G
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood; D000447:Aldehydes / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood; D015153:Blotting, Western; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D004798:Enzymes; D005260:Female; D006525:Hepatitis, Viral, Human / Q000097:blood* / Q000473:pathology / Q000652:urine*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D013879:Thioredoxins / Q000378:metabolism; D015854:Up-Regulation
D000447:Aldehydes; D004798:Enzymes; C045934:4-hydroxy-2-hexenal; D013879:Thioredoxins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003849:Deoxyguanosine; C027576:4-hydroxy-2-nonenal