title: Identification of differentially expressed genes in clear cell renal cell carcinoma by analysis of full-length enriched cDNA library.
abstract: Renal cell carcinoma (RCC) is the most common malignancy in adult kidney, and accounts for 3% of malignancies worldwide with increasing incidence. Clear cell RCC (ccRCC) is the major type in RCC. Resection by surgery is the main treatment because the response of ccRCC to traditional therapies is very poor. To identify the tumor-associated genes for better understanding the molecular mechanism of ccRCC, the full-length enriched cDNA libraries of ccRCC and normal kidney tissues were constructed by the oligo-capping method. Nucleotide sequences of the cDNA libraries of ccRCC and normal kidney tissues were sequenced. From the sequence analysis of 19,425 and 12,400 clones of ccRCC and normal kidney tissues, 4356 and 3055 genes were identified, respectively. By comparing the gene-expression patterns of ccRCC and normal tissues, the up- or down-regulated genes were identified. Among these identified genes, the differential expression of annexin A2 and argininosuccinate synthetase genes were further confirmed by quantitative real-time PCR and Western blot analysis.
authors.affiliation: Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Sai-Wen
authors.initials: SW
authors.lastname: Tang
authors.forename: Wen-Hsin
authors.initials: WH
authors.lastname: Chang
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Chao
authors.forename: Chung-Yei
authors.initials: CY
authors.lastname: Lin
authors.forename: Hsiao-Fen
authors.initials: HF
authors.lastname: Chen
authors.forename: Yen-Han
authors.initials: YH
authors.lastname: Lai
authors.forename: Bo-Wen
authors.initials: BW
authors.lastname: Zhan
authors.forename: Yih-Ching
authors.initials: YC
authors.lastname: Su
authors.forename: Sen-Wen
authors.initials: SW
authors.lastname: Jane
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Chen
authors.forename: Chyong-Ing
authors.initials: CI
authors.lastname: Hsu
authors.forename: Wei-Chou
authors.initials: WC
authors.lastname: Lin
authors.forename: Kao-Chung
authors.initials: KC
authors.lastname: Wang
authors.forename: Ming-Kuen
authors.initials: MK
authors.lastname: Lai
authors.forename: Jung-Yaw
authors.initials: JY
authors.lastname: Lin
mesh_terms: D017306:Annexin A2 / Q000235:genetics; D001124:Argininosuccinate Synthase / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics
chemical_list: D017306:Annexin A2; D009363:Neoplasm Proteins; D001124:Argininosuccinate Synthase