Priming of glomerular mesangial cells by activated macrophages causes blunted responses to proinflammatory stimuli.
Macrophage-mesangial cell interaction plays a crucial role in the pathogenesis of glomerulonephritis. Activated macrophages trigger mesangial cells to express an array of inflammation-associated genes via activation of NF-kappaB and AP-1. However, this inflammatory response is often transient and subsides spontaneously. We found that mesangial cells activated by bystander macrophages showed blunted responses of NF-kappaB to subsequent macrophage exposure. It was associated with sustained levels of IkappaBbeta, but not IkappaBalpha. The tolerance observed was reversible and reproduced by conditioned media from activated macrophages (macrophage-conditioned medium (MphiCM)). In vivo priming of mesangial cells by activated glomerular macrophages also caused the tolerance of mesangial cells. The macrophage-derived tolerance inducers were heat-labile, and multiple molecules were involved. Among inflammatory cytokines produced by macrophages, TNF-alpha and IL-1beta were able to induce mesangial cell tolerance dose-dependently. The mesangial cell tolerance was also observed in activation of the MAPK-AP-1 pathway; i.e., phosphorylation of ERK, JNK, and p38 MAPK by macrophages was blunted when the cells were pre-exposed to MphiCM. Induction of c-fos and c-jun was also abrogated in mesangial cells pre-exposed to MphiCM, and the suppression was attenuated by blockade of MAPK activation during the first exposure to MphiCM. These data elucidated that mesangial cells, once exposed to macrophages, become insensitive to subsequent activation by macrophages and proinflammatory stimuli. This self defense of glomerular cells may play a role in the resolution of macrophage-mediated, acute glomerulonephritis.
Hayakawa
Kunihiro
K
Department of Molecular Signaling, University of Yamanashi, Japan.
Meng
Yiman
Y
Hiramatsu
Nobuhiko
N
Kasai
Ayumi
A
Yamauchi
Kozue
K
Yao
Jian
J
Kitamura
Masanori
M
D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D051550:I-kappa B Kinase / Q000378:metabolism; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D018836:Inflammation Mediators / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D050527:Mesangial Cells / Q000276:immunology* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D051381:Rats; D012995:Solubility; D018808:Transcription Factor AP-1 / Q000378:metabolism
D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D051550:I-kappa B Kinase; D020928:Mitogen-Activated Protein Kinases