title: Disruption of neuromedin B receptor gene results in dysregulation of the pituitary-thyroid axis.
abstract: The level of thyrotropin (TSH) secretion is determined by the balance of TSH-releasing hormone (TRH) and thyroid hormones. However, neuromedin B (NB), a bombesin-like peptide, highly concentrated in the pituitary, has been postulated to be a tonic inhibitor of TSH secretion. We studied the pituitary-thyroid axis in adult male mice lacking NB receptor (NBR-KO) and their wild-type (WT) littermates. At basal state, NBR-KO mice presented serum TSH slightly higher than WT (18%, P< 0.05), normal intra-pituitary TSH content, and no significant changes in alpha and beta TSH mRNA levels. Serum thyroxine was normal but serum triiodothyronine (T3) was reduced by 24% (P< 0.01) in NBR-KO mice. Pituitaries of NBR-KO mice exhibited no alteration in prolactin mRNA expression but type I and II deiodinase mRNA levels were reduced by 53 and 42% respectively (P< 0.05), while TRH receptor mRNA levels were importantly increased (78%, P< 0.05). The TSH-releasing effect of TRH was significantly higher in NBR-KO than in WT mice (7.1-and 4.0-fold respectively), but, while WT mice presented a 27% increase in serum T3 (P< 0.05) after TRH, NBR-KO mice showed no change in serum T3 after TRH. NBR-KO mice did not respond to exogenous NB, while WT showed a 30% reduction in serum TSH. No compensatory changes in mRNA expression of NB or other bombesin-related peptides and receptors (gastrin-releasing peptide (GRP), GRP-receptor and bombesin receptor subtype-3) were found in the pituitary of NBR-KO mice. Therefore, the data suggest that NB receptor pathways are importantly involved in thyrotroph gene regulation and function, leading to a state where TSH release is facilitated especially in response to TRH, but probably with a less-bioactive TSH. Therefore, the study highlights the important role of NB as a physiological regulator of pituitary-thyroid axis function and gene expression.
authors.affiliation: Laboratório de Endocrinologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil.
authors.forename: K J
authors.initials: KJ
authors.lastname: Oliveira
authors.forename: T M
authors.initials: TM
authors.lastname: Ortiga-Carvalho
authors.forename: A
authors.initials: A
authors.lastname: Cabanelas
authors.forename: M A L C
authors.initials: MA
authors.lastname: Veiga
authors.forename: K
authors.initials: K
authors.lastname: Aoki
authors.forename: H
authors.initials: H
authors.lastname: Ohki-Hamazaki
authors.forename: K
authors.initials: K
authors.lastname: Wada
authors.forename: E
authors.initials: E
authors.lastname: Wada
authors.forename: C C
authors.initials: CC
authors.lastname: Pazos-Moura
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010902:Pituitary Gland / Q000502:physiology*; D010907:Pituitary Hormones / Q000097:blood / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D018004:Receptors, Bombesin / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013961:Thyroid Gland / Q000502:physiology*; D013963:Thyroid Hormones / Q000502:physiology
chemical_list: D017931:DNA Primers; D010907:Pituitary Hormones; D012333:RNA, Messenger; D018004:Receptors, Bombesin; D013963:Thyroid Hormones