Echinococcus multilocularis: developmental stage-specific expression of Antigen B 8-kDa-subunits.
Antigen B (AgB) initially found in hydatid cyst fluid of Echinococcus granulosus is a polymeric lipoprotein of 160 kDa, and is an aggregate of several different but homologous small proteins with approximately 8 kDa. Four genes encoding these 8-kDa-subunits have been identified from E. granulosus. In this study we isolated five genes encoding 8-kDa-subunits of AgB from Echinococcus multilocularis. Sequence comparison of isolated cDNA clones demonstrated that one of these five clones was completely identical to EmAgB8/1 which had been isolated previously by our group, and three of them were 94.5, 90.8, and 91.9% homologous to E. granulosus antigen B 8-kDa subunit genes, EgAgB8/2, EgAgB8/3, and EgAgB8/4, respectively. The remaining clone shared 51-58% homology with the nucleotide sequences of AgB genes. Gene-specific RT-PCR and Western blot analyses revealed that these genes were expressed in a developmentally regulated manner in E. multilocularis vesicles, protoscoleces, and immature adult worms. Possible functions of different expression manners are also discussed.
Mamuti
Wulamu
W
Department of Parasitology, Asahikawa Medical College, Japan.
Sako
Yasuhito
Y
Xiao
Ning
N
Nakaya
Kazuhiro
K
Nakao
Minoru
M
Yamasaki
Hiroshi
H
Lightowlers
Marshall W
MW
Craig
Philip S
PS
Ito
Akira
A
D000595:Amino Acid Sequence; D000818:Animals; D003411:Arvicolinae; D001483:Base Sequence; D015153:Blotting, Western; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D004285:Dogs; D048210:Echinococcus multilocularis / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D018507:Gene Expression Regulation, Developmental; D015801:Helminth Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D008074:Lipoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D018510:RNA, Helminth / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D008074:Lipoproteins; D018510:RNA, Helminth; C089273:antigen B, Echinococcus