Immobilization of antibodies on biosensing devices by nanoarrayed self-assembled monolayers.
This work presents an original and straightforward technique for antibody immobilization onto a surface, keeping the antibody in a biologically reactive configuration. Self-assembly of molecular monolayers and plasma-based colloidal lithography were combined to create chemical nanopatterns on the surface of a biosensing device. This technique was employed to create an array of 100 nm wide motifs having a hexagonal 2-D crystalline structure, characterized by COOH-terminated nanospots in a CH3-terminated matrix. The quality control of the chemical nanopattern was carried out by combining atomic force microscopy, ellipsometry, and contact angle measurements. Enzyme-linked immunosorbent assay experiments were set up showing that the COOH/CH3 nanopatterned surface constrains the immobilization of the antibodies in a biologically reactive configuration, thus significantly improving the device performances as compared to those of more conventional nonpatterned COOH-terminated or CH3-terminated surfaces.
Valsesia
Andrea
A
Institute for Health and Consumer Protection, Joint Research Centre, European Commission, Via E. Fermi 1, 21020 Ispra (Varese), Italy. andrea.valsesia@jrc.it
Colpo
Pascal
P
Meziani
Tarik
T
Lisboa
Patrícia
P
Lejeune
Michaël
M
Rossi
François
F
D000327:Adsorption; D000906:Antibodies / Q000737:chemistry*; D015374:Biosensing Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry*; D013499:Surface Properties
D000906:Antibodies; D008567:Membranes, Artificial