title: High-throughput multi-antigen microfluidic fluorescence immunoassays.
abstract: Here we describe the development of a high-throughput multi-antigen microfluidic fluorescence immunoassay system. A 100-chamber polydimethylsiloxane (PDMS) chip performs up to 5 tests for each of 10 samples. In this particular study system, the specificity of detection was demonstrated, and calibration curves were produced for C-reactive protein (CRP), prostate-specific antigen (PSA), ferritin, and vascular endothelial growth factor (VEGF). The measurements show sensitivity at and below clinically normal levels (with a signal-to-noise ratio >8 at as low as 10 pM antigen concentration). The chip uses 100 nL per sample for all tests. The developed system is an important step toward derivative immunoassay applications in scientific research and "point-of-care" testing in medicine.
authors.affiliation: Dept of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles 90033, USA. kartalov@usc.edu
authors.forename: Emil P
authors.initials: EP
authors.lastname: Kartalov
authors.forename: Jiang F
authors.initials: JF
authors.lastname: Zhong
authors.forename: Axel
authors.initials: A
authors.lastname: Scherer
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Quake
authors.forename: Clive R
authors.initials: CR
authors.lastname: Taylor
authors.forename: W French
authors.initials: WF
authors.lastname: Anderson
mesh_terms: D000941:Antigens / Q000032:analysis*; D002097:C-Reactive Protein / Q000032:analysis; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D005293:Ferritins / Q000097:blood; D015200:Fluoroimmunoassay / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization; D017430:Prostate-Specific Antigen / Q000097:blood; D012828:Silicones / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: D000941:Antigens; D004129:Dimethylpolysiloxanes; D012828:Silicones; D042461:Vascular Endothelial Growth Factor A; C013830:baysilon; D002097:C-Reactive Protein; D005293:Ferritins; D017430:Prostate-Specific Antigen