title: N-t-Butyl hydroxylamine regulates heat shock-induced apoptosis in U937 cells.
abstract: Heat shock may increase oxidative stress due to increased production of reactive oxygen species and/or the promotion of cellular oxidation events. Therefore, compounds that scavenge reactive oxygen species may regulate heat shock-induced cell death. Recently, it has been shown that the decomposition product of the spin-trapping agent alpha-phenyl-N-t-butylnitrone, N-t-butyl hydroxylamine (NtBHA), mimics alpha-phenyl-N-t-butylnitrone and is much more potent in delaying reactive oxygen species-associated senescence. We investigated the protective role of NtBHA against heat shock-induced apoptosis in U937 cells. Upon exposure to heat shock, there was a distinct difference between the untreated cells and the cells pre-treated with 0.1 mM NtBHA for 2 h in regard to apoptotic parameters, cellular redox status, and mitochondrial function. Upon exposure to heat shock, NtBHA pre-treated cells showed significant inhibition of apoptotic features such as activation of caspase-3, up-regulation of Bax, and down-regulation of Bcl-2 compared to untreated cells. This study indicates that NtBHA may play an important role in regulating the apoptosis induced by heat shock, presumably through scavenging of reactive oxygen species.
authors.affiliation: School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu, Korea.
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Seoung Woo
authors.initials: SW
authors.lastname: Shin
authors.forename: Chang Joo
authors.initials: CJ
authors.lastname: Oh
authors.forename: Mann Hyung
authors.initials: MH
authors.lastname: Lee
authors.forename: Chae Ha
authors.initials: CH
authors.lastname: Yang
authors.forename: Jeen-Woo
authors.initials: JW
authors.lastname: Park
mesh_terms: D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006358:Hot Temperature; D006801:Humans; D006898:Hydroxylamines / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D017382:Reactive Oxygen Species; D013113:Spin Labels; D013997:Time Factors; D020298:U937 Cells
chemical_list: D006898:Hydroxylamines; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D013113:Spin Labels; C072771:N-tert-butylhydroxylamine; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases