title: A new specific gene expression in squamous cell carcinoma of the esophagus detected using representational difference analysis and cDNA microarray.
abstract: OBJECTIVE
  To detect new specific gene expressions in squamous cell carcinoma of the esophagus.
  
  
  METHODS
  Representational difference analysis of cDNA (cDNA RDA) was applied to a human esophageal cancer cell line (KYSE170) and a human esophageal epithelial cell line (HEEC-1).
  
  
  RESULTS
  LAGE-1 was expressed specifically in KYSE170, but not in HEEC-1. It is also expressed in 27% of esophageal cancer cell lines (3/11) and 33% of esophageal cancer tissues (10/30), but not in other HEECs, normal esophageal epithelium, or other normal tissues except testis, ovary and kidney. The expression of LAGE-1 is strongly correlated with that of MAGE-A1 (p = 0.013, Fisher's exact probability test). Fibronectin, cytokeratin 6B, cytokeratin 19, cyclin D2 and Ten-m2 were detected as candidates for downregulated genes. Reduced expression profiles of them were also identified using cDNA microarrays. The expression of LAGE-1 was induced by 5'-aza-2'-deoxycytidine (5Aza-dC) and trichostatin A (TSA) in esophageal cancer cell lines, which did not express LAGE-1. In HEECs, 5Aza-dC induced LAGE-1 expression, but TSA did not.
  
  
  CONCLUSIONS
  LAGE-1 expression was detected in esophageal cancer by cDNA RDA. LAGE-1 might have the potential to be a target antigen for anti-tumoral immunotherapy in esophageal cancers because of its tumor-specific expression similar to that of MAGE-A1.
authors.affiliation: Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Sakyo-ku, Japan.
authors.forename: Takatsugu
authors.initials: T
authors.lastname: Kan
authors.forename: Seiji
authors.initials: S
authors.lastname: Yamasaki
authors.forename: Kan
authors.initials: K
authors.lastname: Kondo
authors.forename: Naoki
authors.initials: N
authors.lastname: Teratani
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawabe
authors.forename: Junichi
authors.initials: J
authors.lastname: Kaganoi
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Meltzer
authors.forename: Masayuki
authors.initials: M
authors.lastname: Imamura
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Shimada
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000235:genetics*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004847:Epithelial Cells; D004938:Esophageal Neoplasms / Q000737:chemistry / Q000235:genetics*; D004947:Esophagus / Q000737:chemistry / Q000166:cytology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D014408:Biomarkers, Tumor; C104948:CTAG1B protein, human; C510162:CTAG2 protein, human; D008565:Membrane Proteins