Microtubule-dependent relocation of branchial V-H+-ATPase to the basolateral membrane in the Pacific spiny dogfish (Squalus acanthias): a role in base secretion.
We have previously shown that continuous intravenous infusion of NaHCO3 for 24 h ( approximately 1000 micromol kg(-1) h(-1)) results in the relocation of V-H+-ATPase from the cytoplasm to the basolateral membrane in the gills of the Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with the addition of colchicine, an inhibitor of cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO2 were significantly higher in the colchicines-treated, HCO3- -infused fish compared with fish infused with HCO3- alone. The effect of colchicine was highest after 24 h of infusion (8.33+/-0.06 vs 8.02+/-0.03 pH units, 15.72+/-3.29 vs 6.74+/-1.34 mmol CO2 l(-1), N=5). Immunohistochemistry and western blotting confirmed that colchicine blocked the transit of V-H+-ATPase to the basolateral membrane. Furthermore, western blotting analyses from whole gill and cell membrane samples suggest that the short-term (6 h) response to alkaline stress consists of relocation of V-H+-ATPases already present in the cell to the basolateral membrane, while in the longer term (24 h) there is both relocation of preexistent enzyme and upregulation in the synthesis of new units. Our results strongly suggest that cellular relocation of V-H+-ATPase is necessary for enhanced HCO3- secretion across the gills of the Pacific dogfish.
Tresguerres
Martin
M
Dept of Biological Sciences, University of Alberta, Edmonton, Alberta, T5G 2E9, Canada. martint@ualberta.ca
Parks
Scott K
SK
Katoh
Fumi
F
Goss
Greg G
GG
D000136:Acid-Base Equilibrium; D000818:Animals; D002245:Carbon Dioxide / Q000097:blood; D016764:Cell Polarity; D003078:Colchicine / Q000494:pharmacology; D004334:Drug Administration Schedule; D005880:Gills / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008870:Microtubules / Q000378:metabolism*; D021381:Protein Transport; D017693:Sodium Bicarbonate / Q000378:metabolism; D012965:Sodium Chloride; D048251:Squalus acanthias / Q000097:blood; D050257:Tubulin Modulators / Q000494:pharmacology; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism*
D050257:Tubulin Modulators; D002245:Carbon Dioxide; D012965:Sodium Chloride; D017693:Sodium Bicarbonate; D025262:Vacuolar Proton-Translocating ATPases; D003078:Colchicine