title: Catalytic effects of elongation factor Ts on polypeptide synthesis.
abstract: The kinetic parameters which characterize the interaction between elongation factor Tu (EF-Tu) and elongation factor Ts (EF-Ts) have been determined in a poly(uridylic acid)-primed translation system. The EF-Ts catalyzed release of GDP from EF-Tu was measured independently in a nucleotide exchange assay. We conclude that the rate-limiting step for the EF-Tu cycle in protein synthesis in the absence of EF-Ts is the release of GDP. By adding EF-Ts the time of this step is reduced from 90 s to 30 ms. Half maximal rate is obtained at an EF-Ts concentration of 2.5 x 10 M.
authors.affiliation: Department of Molecular Biology, Biomedicum, Box 590, University of Uppsala, S-751 24 Uppsala, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Ruusala
authors.forename: M
authors.initials: M
authors.lastname: Ehrenberg
authors.forename: C G
authors.initials: CG
authors.lastname: Kurland