title: Off-line combination of reversed-phase liquid chromatography and laser desorption/ionization time-of-flight mass spectrometry with seamless post-source decay fragment ion analysis for characterization of square-planar nickel(II) complexes.
abstract: Characterization of square-planar nickel(II) complexes of the Schiff base of (S)-N-benzylproline (2-benzoylphenyl)amide and various amino acids that are used as efficient alpha-amino acids synthons was carried out using laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS) in off-line combination with liquid chromatography. A mixture of four square-planar nickel(II) complexes was separated using reversed-phase liquid chromatography (RPLC) and the separated fractions from the chromatographic run were spotted on the metal target directly from the column outlet using a lab-made sample deposition device. The separated fractions were then analyzed by LDI-TOF MS. Seamless postsource decay (sPSD) fragment ion analysis was used for their structural characterization, which made possible the confirmation of expected chemical structures of the analyzed compounds. The off-line combination of the separation by RPLC and analysis by LDI-TOF MS allowed successful separation, sensitive detection and structure elucidation of the square-planar nickel(II) complexes.
authors.affiliation: Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veverí 97, 611 42 Brno, Czech Republic. rehulka@iach.cz
authors.forename: Pavel
authors.initials: P
authors.lastname: Rehulka
authors.forename: Alexander
authors.initials: A
authors.lastname: Popkov
authors.forename: Milan
authors.initials: M
authors.lastname: Nádvorník
authors.forename: Josef
authors.initials: J
authors.lastname: Planeta
authors.forename: Karel
authors.initials: K
authors.lastname: Mazanec
authors.forename: Josef
authors.initials: J
authors.lastname: Chmelík