Antibacterial activity of components from Lomatium californicum.
The isolation, characterization and bioactivity testing of compounds from Lomatium californicum (Nutt.) are described. Ethyl acetate and hexane extracts of the roots of L. californicum were subjected to vacuum liquid chromatography (VLC), flash column chromatography (FCC) and separation by normal- and reverse-phase high-performance liquid chromatography (HPLC). Six compounds were isolated successfully and characterized by 1D and 2D nuclear magnetic resonance (NMR) experimentation. The bioactivity of the known compounds (+)-falcarindiol, coniferyl ferulate, ferulic acid and (Z)-ligustilide were confirmed against the Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus. The known compounds senkyunolide I and trans-neocnidilide were also isolated but in too small a quantity for similar testing.
Chou
Shen-Chieh
SC
Department of Chemistry, Sweet Briar College, Sweet Briar, VA 24595, USA.
Everngam
Molly C
MC
Sturtz
George
G
Beck
John J
JJ
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019661:Apiaceae / Q000737:chemistry*; D001412:Bacillus subtilis / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D010936:Plant Extracts