Directed DNA metallization.
Genes of interest can be selectively metallized via the incorporation of modified triphosphates. These triphosphates bear functions that can be further derivatized with aldehyde groups via the use of click chemistry. Treatment of the aldehyde-labeled gene mixture with the Tollens reagent, followed by a development process, results in the selective metallization of the gene of interest in the presence of natural DNA strands.
Burley
Glenn A
GA
Department of Chemistry, Technion, Haifa, Israel.
Gierlich
Johannes
J
Mofid
Mohammad R
MR
Nir
Hadar
H
Tal
Shay
S
Eichen
Yoav
Y
Carell
Thomas
T
D018076:DNA, Complementary / Q000737:chemistry*; D004271:DNA, Fungal / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D006801:Humans; D016133:Polymerase Chain Reaction; D012834:Silver / Q000737:chemistry*; D000099270:Y-Family DNA Polymerases
D018076:DNA, Complementary; D004271:DNA, Fungal; D004259:DNA-Directed DNA Polymerase; D012834:Silver; C112338:Rad30 protein; D000099270:Y-Family DNA Polymerases