title: Identification of novel biomarker candidates by differential peptidomics analysis of cerebrospinal fluid in Alzheimer's disease.
abstract: The objective of this work was the application of peptidomics technologies for the detection and identification of reliable and robust biomarkers for Alzheimer's disease (AD) contributing to facilitate and further improve the diagnosis of AD. Using a new method for the comprehensive and comparative profiling of peptides, the differential peptide display (DPD), 312 cerebrospinal fluid (CSF) samples from AD patients, cognitively unimpaired subjects and from patients suffering from other primary dementia disorders were analysed as four independent analytical sets. By combination with a cross validation procedure, candidates were selected from a total of more than 6,000 different peptide signals based on their discriminating power. Twelve candidates were identified using mass-spectrometric techniques as fragments of the possibly neuroprotective neuroendocrine protein VGF and another one as the complement factor C3 descendent C3f. The combination of peptide profiling and cross validation resulted in the detection of novel potential biomarkers with remarkable robustness and a close relation to AD pathophysiology.
authors.affiliation: BioVisioN AG, 30625 Hannover, Germany. h.selle@peptidomics.de
authors.forename: Hartmut
authors.initials: H
authors.lastname: Selle
authors.forename: Jens
authors.initials: J
authors.lastname: Lamerz
authors.forename: Katharina
authors.initials: K
authors.lastname: Buerger
authors.forename: Andreas
authors.initials: A
authors.lastname: Dessauer
authors.forename: Klaus
authors.initials: K
authors.lastname: Hager
authors.forename: Harald
authors.initials: H
authors.lastname: Hampel
authors.forename: Johann
authors.initials: J
authors.lastname: Karl
authors.forename: Markus
authors.initials: M
authors.lastname: Kellmann
authors.forename: Lars
authors.initials: L
authors.lastname: Lannfelt
authors.forename: Jukka
authors.initials: J
authors.lastname: Louhija
authors.forename: Matthias
authors.initials: M
authors.lastname: Riepe
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Rollinger
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Tumani
authors.forename: Michael
authors.initials: M
authors.lastname: Schrader
authors.forename: Hans-Dieter
authors.initials: HD
authors.lastname: Zucht
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008956:Models, Chemical; D008969:Molecular Sequence Data; D036103:Nanotechnology; D016571:Neural Networks, Computer; D010447:Peptide Hydrolases / Q000737:chemistry; D010455:Peptides / Q000134:cerebrospinal fluid* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010455:Peptides; D010447:Peptide Hydrolases