Optical diagnosis of cervical cancer by fluorescence spectroscopy technique.
In the present work, we examine normal and malignant stage IIIB cervical tissue by laser induced fluorescence, with 2 different objectives. (i) Development of the fluorescence spectroscopy technique as a standard optical method for discrimination of normal and malignant tissue samples and, (ii) Optimization of the technique by the method of matching of a sample spectrum with calibration sets of spectra of pathologically certified samples. Laser-induced fluorescence spectra were measured using samples from 62 subjects at different excitation wavelengths. Principal component analysis (PCA) of spectra and intensity ratios of curve-resolved fluorescence peaks were tested for discrimination. It was found that PCA of total fluorescence at 325 nm excitation gives specificity and sensitivity over 95%. Use of calibration sets of spectra of histo-pathologically certified samples combined with PCA for matching and pass/fail classification of test samples is shown to have high sensitivity/specificity for routine diagnostic purposes as well as for possible staging of the disease. Further, the multi-component origin of the fluorescence spectra is illustrated by curve resolution and fluorescence spectra of separated proteins of tissue homogenates.
Chidananda
Siddappa M
SM
Center for Molecular and Cellular Biology, Manipal Academy of Higher Education, Science Center, Manipal, Karnataka, India.
Satyamoorthy
Kapaettu
K
Rai
Lavanya
L
Manjunath
Attibele P
AP
Kartha
Vasudevan B
VB
D000328:Adult; D002138:Calibration; D005260:Female; D006801:Humans; D007834:Lasers; D008875:Middle Aged; D009367:Neoplasm Staging; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000473:pathology