title: The dopaminergic midbrain participates in human episodic memory formation: evidence from genetic imaging.
abstract: Recent data from animal studies raise the possibility that dopaminergic neuromodulation promotes the encoding of novel stimuli. We investigated a possible role for the dopaminergic midbrain in human episodic memory by measuring how polymorphisms in dopamine clearance pathways affect encoding-related brain activity (functional magnetic resonance imaging) in an episodic memory task. In 51 young, healthy adults, successful episodic encoding was associated with activation of the substantia nigra. This midbrain activation was modulated by a functional variable number of tandem repeat (VNTR) polymorphism in the dopamine transporter (DAT1) gene. Despite no differences in memory performance between genotype groups, carriers of the (low expressing) 9-repeat allele of the DAT1 VNTR showed relatively higher midbrain activation when compared with subjects homozygous for the 10-repeat allele, who express DAT1 at higher levels. The catechol-O-methyl transferase (COMT) Val108/158Met polymorphism, which is known to modulate enzyme activity, affected encoding-related activity in the right prefrontal cortex (PFC) and in occipital brain regions but not in the midbrain. Moreover, subjects homozygous for the (low activity) Met allele showed stronger functional coupling between the PFC and the hippocampus during encoding. Our finding that genetic variations in the dopamine clearance pathways affect encoding-related activation patterns in midbrain and PFC provides strong support for a role of dopaminergic neuromodulation in human episodic memory formation. It also supports the hypothesis of anatomically and functionally distinct roles for DAT1 and COMT in dopamine metabolism, with DAT1 modulating rapid, phasic midbrain activity and COMT being particularly involved in prefrontal dopamine clearance.
authors.affiliation: Department of Neurology II, Center for Advanced Imaging, University Hospital of Magdeburg, 39120 Magdeburg, Germany. bschott@neuro2.med.uni-magdeburg.de
authors.forename: Björn H
authors.initials: BH
authors.lastname: Schott
authors.forename: Constanze I
authors.initials: CI
authors.lastname: Seidenbecher
authors.forename: Daniela B
authors.initials: DB
authors.lastname: Fenker
authors.forename: Corinna J
authors.initials: CJ
authors.lastname: Lauer
authors.forename: Nico
authors.initials: N
authors.lastname: Bunzeck
authors.forename: Hans-Gert
authors.initials: HG
authors.lastname: Bernstein
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Tischmeyer
authors.forename: Eckart D
authors.initials: ED
authors.lastname: Gundelfinger
authors.forename: Hans-Jochen
authors.initials: HJ
authors.lastname: Heinze
authors.forename: Emrah
authors.initials: E
authors.lastname: Düzel
mesh_terms: D000293:Adolescent; D000328:Adult; D001519:Behavior; D001921:Brain / Q000033:anatomy & histology / Q000502:physiology; D002394:Catechol O-Methyltransferase / Q000235:genetics*; D004298:Dopamine / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005838:Genotype; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D008568:Memory / Q000502:physiology*; D011939:Mental Recall; D008636:Mesencephalon / Q000033:anatomy & histology / Q000502:physiology*; D011110:Polymorphism, Genetic; D017397:Prefrontal Cortex / Q000502:physiology; D020080:Tandem Repeat Sequences
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; C493214:SLC6A3 protein, human; D002394:Catechol O-Methyltransferase; D004298:Dopamine