title: Transcranial imaging of audiogenic epileptic foci in the cortex of DBA/2J mice.
abstract: Exposure to intense sound stimuli induces audiogenic seizures in DBA/2J mice. We investigated cortical activities during sound stimulation using flavoprotein fluorescence imaging. Most DBA/2J mice had seizures during intense sound stimulation, with more than half surviving after seizures. Surviving mice were anesthetized with urethane (1.6 g/kg, intraperitoneal), and the skull was exposed and then covered with clear resin. More than 3 days after surgery, the mice were lightly anesthetized with urethane (0.8 g/kg) and cortical activities during intense sound stimulation were visualized. Focal responses appeared near the somatosensory cortex together with spike activities localized in the response area. These findings indicate that epileptic foci of audiogenic seizure are formed in the cortex of DBA/2J mice.
authors.affiliation: Department of Neurophysiology, Brain Research Institute, Niigata University, Niigata, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Takao
authors.forename: Hiroatsu
authors.initials: H
authors.lastname: Murakami
authors.forename: Masafumi
authors.initials: M
authors.lastname: Fukuda
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Kakita
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Takahashi
authors.forename: Masaharu
authors.initials: M
authors.lastname: Kudoh
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Tanaka
authors.forename: Katsuei
authors.initials: K
authors.lastname: Shibuki
mesh_terms: D000161:Acoustic Stimulation / Q000009:adverse effects*; D000818:Animals; D001931:Brain Mapping; D003952:Diagnostic Imaging / Q000379:methods*; D020195:Epilepsy, Reflex / Q000209:etiology* / Q000503:physiopathology; D005072:Evoked Potentials, Auditory / Q000502:physiology*; D005420:Flavoproteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013003:Somatosensory Cortex / Q000503:physiopathology*; D013045:Species Specificity; D013997:Time Factors
chemical_list: D005420:Flavoproteins