Epididymal genomics and the search for a male contraceptive.
This report represents the joint efforts of three laboratories, one with a primary interest in understanding regulatory processes in the epididymal epithelium (TTT) and two with a primary interest in identifying and characterizing new contraceptive targets (DSJ and SAJ). We have developed a highly refined mouse epididymal transcriptome and have used it as a starting point for determining genes in the human epididymis, which may serve as targets for male contraceptives. Our database represents gene expression information for approximately 39,000 transcripts, of which over 17,000 are significantly expressed in at least one segment of the mouse epididymis. Over 2000 of these transcripts are up- or down-regulated by at least four-fold between at least two segments. In addition, human databases have been queried to determine expression of orthologs in the human epididymis and the specificity of their expression in the epididymis. Genes highly regulated in the human epididymis and showing high tissue specificity are potential targets for male contraceptives.
Turner
T T
TT
Department of Urology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA. ttt@virgnia.edu
Johnston
D S
DS
Jelinsky
S A
SA
D000818:Animals; D019295:Computational Biology; D003272:Contraceptive Agents, Male / Q000494:pharmacology; D004822:Epididymis / Q000187:drug effects* / Q000378:metabolism*; D005306:Fertilization / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation; D023281:Genomics; D008297:Male; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003272:Contraceptive Agents, Male