[Study on effects of salidroside on lipid peroxidation on oxidative stress in rat hepatic stellate cells].
OBJECTIVE
To investigate the effect of antioxidant Salidroside on the lipid peroxidation of oxidative stress in rat hepatic stellate cells in vitro.


METHODS
HSC-T6 were incubated with 0.1 mM ferric nitrilotriacetate complex (FeNTA). MTT colorimetry was used for assaying proliferation of HSC. Cytotoxicity was measured by LDH colorimetry. Collagen type I accummulation in the culture media was measured by ELISA. Intracellular MDA, SOD, GSH and GSH-PX levels in the culture media were measured by their reagent boxes.


RESULTS
HSC incubation with FeNTA resulted in a significant production of intracellular MDA and GSH, associated with decreasing SOD and GSH-PX activity. Exposure of HSC to FeNTA significantly enhanced the number of proliferating HSC and collagen type I levels in the culture medium. All these effects were reversed by the antioxidant Salidroside.


CONCLUSIONS
The mechanism of Salidroside on fibrosis may be related to inhibit lipid peroxidation.
Zhang
Yi
Y
The South China University of Technology Hospital, Guangzhou, 510640.
Liu
Yonggang
Y
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D005960:Glucosides / Q000008:administration & dosage / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D029771:Rhodiola / Q000737:chemistry
D024042:Collagen Type I; D005960:Glucosides; D010636:Phenols; C009172:rhodioloside