title: Influence of polyelectrolyte multilayer films on the ICAM-1 expression of endothelial cells.
abstract: Recently, the use of polyelectrolyte films has been suggested as a new versatile technique of surface modification aimed at tissue engineering. In the present study, we evaluated the expression of intercellular adhesion molecule (ICAM)-1 of endothelial cells (ECs) seeded on two types of polyelectrolyte multilayer films either terminated by poly(D-lysine) (PDL) or poly(allylamine hydrochloride) (PAH). This work showed that chemical stimulations with tumor necrosis factor (TNF)-alpha induced the ICAM-1 expression of ECs differently depending largely on the film architecture employed. Compared with PAH-ending films, the PDL-ending ones upregulated the ICAM-1 expression of the ECs after a prolonged exposition to TNF-alpha, rendering this film type less favorable in tissue engineering. Cytochalasin D (an F-actin disrupting agent) showed the involvement of the cytoskeleton in the upregulation of ICAM-1 for cells deposited on films terminated by PDL. The PAH-ending films did not perturb the ICAM-1 expression of ECs and might thus enhance the seeding of ECs in vascular engineering.
authors.affiliation: Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA-UMR CNRS 7563, Faculté de Médecine, 54505 Vandoeuvre-lès-Nancy, France. cedric.boura@medecine.uhp-nancy.fr
authors.forename: Cédric
authors.initials: C
authors.lastname: Boura
authors.forename: Sylvaine
authors.initials: S
authors.lastname: Muller
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Voegel
authors.forename: Pierre
authors.initials: P
authors.lastname: Schaaf
authors.forename: Jean François
authors.initials: JF
authors.lastname: Stoltz
authors.forename: Patrick
authors.initials: P
authors.lastname: Menu
mesh_terms: D002478:Cells, Cultured; D015638:Cytochalasin D / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D011073:Polyamines; D000071228:Polyelectrolytes; D013314:Stress, Mechanical; D013499:Surface Properties; D023822:Tissue Engineering; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D015638:Cytochalasin D; D018799:Intercellular Adhesion Molecule-1; D011073:Polyamines; D000071228:Polyelectrolytes; D014409:Tumor Necrosis Factor-alpha