Damage of cell membrane and antioxidative system in human erythrocytes incubated with microcystin-LR in vitro.
The effect of the exposure of human erythrocytes to different concentrations of microcystin-LR were studied. Lipid peroxidation, membrane fluidity, cell morphology, haemoglobin oxidation and changes in the activity of antioxidant enzymes were investigated. Human erythrocytes were incubated with microcystin-LR at concentrations of 1-1000 nM for 1, 6, 12 and 24 h. We observed that microcystin-LR induces a significant increase of the level of thiobarbituric acid reactive substances (TBARS), formation of echinocytes, haemolysis, conversion of oxyhaemoglobin to methaemoglobin, decrease of membrane fluidity on the level of 16 carbon atom fat acids. The compound also changed antioxidative enzymes activities: catalase, superoxide dismutase and glutathione reductase and formation of reactive oxygen species (ROS). All of the observed changes point out that 100 nM of Microcistin LR is the liminal (threshold) toxic dose for human erythrocytes. This dose caused most of the described changes. Observed damages of erythrocytes membrane and antioxidative enzymes may be the result of direct covalent binding of microcystin-LR with -SH residues of proteins and indirectly be related with reactive oxygen species formation.
Sicińska
Paulina
P
Department of Biophysics Environmental Pollution, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Bukowska
Bozena
B
Michałowicz
Jaromir
J
Duda
Wirgiliusz
W
D000975:Antioxidants / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic / Q000633:toxicity*
D000975:Antioxidants; D004791:Enzyme Inhibitors; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C057862:cyanoginosin LR