title: Epstein-Barr virus-associated posttransplantation lymphoproliferative disorder following lung transplantation is more commonly of host origin.
abstract: BACKGROUND
  Posttransplantation lymphoproliferative disorder (PTLD) in patients who have undergone solid organ transplantation is thought to be mostly of host (ie, transplant recipient) origin, as opposed to being predominantly of donor origin, which is observed in patients who have undergone bone marrow transplantation. Donor-origin PTLDs reportedly follow a more indolent course than host-origin PTLDs.
  
  
  OBJECTIVE
  To determine the origin of PTLD and its clinical implications in patients who have undergone lung transplantation.
  
  
  METHODS
  Patients' medical records were reviewed for clinical data. We performed a molecular study to determine the origin of abnormal lymphoid cells in 4 PTLD cases identified from our autopsy files. Each case underwent restriction fragment length polymorphism analysis using polymerase chain reaction-based genotyping for CYP2D6. Epstein-Barr virus (latent membrane protein 1) immunostaining and polymerase chain reaction analysis were performed on PTLD-involved tissues.
  
  
  RESULTS
  Three of 4 PTLD cases were of host origin, and the remaining case was of donor origin. Epstein-Barr virus was detected by immunohistochemical and polymerase chain reaction methods in all PTLD-involved tissues that were examined. There was no apparent difference in clinical manifestations between host-origin and donor-origin PTLD cases in our study.
  
  
  CONCLUSIONS
  The PTLDs in our patients who had undergone lung transplantation were Epstein-Barr virus-positive and mostly of host origin, without any notable clinical difference from donor-origin PTLD.
authors.affiliation: Department of Pathology, University of California, Medical Center, San Diego, CA 92103-8720, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Peterson
authors.forename: Shawn C
authors.initials: SC
authors.lastname: Emery
authors.forename: Gordon L
authors.initials: GL
authors.lastname: Yung
authors.forename: Eliezer
authors.initials: E
authors.lastname: Masliah
authors.forename: Eunhee S
authors.initials: ES
authors.lastname: Yi
mesh_terms: D000368:Aged; D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D020031:Epstein-Barr Virus Infections / Q000150:complications* / Q000473:pathology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D016867:Immunocompromised Host; D016040:Lung Transplantation / Q000009:adverse effects* / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D011183:Postoperative Complications; D014019:Tissue Donors; D014763:Viral Matrix Proteins / Q000378:metabolism
chemical_list: D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins; D019389:Cytochrome P-450 CYP2D6