title: HLA-A, -B, and -DRB1 polymorphism defined by sequence-based typing of the Han population in Northern China.
abstract: DNA typing for human leukocyte antigen (HLA)-A, -B and -DRB1 was performed using polymerase chain reaction-sequence-based typing method on 618 randomly selected healthy individuals of the Han population in Northern China. Allele frequencies and haplotypes were statistically analyzed. A total of 84 HLA-A alleles, 143 B alleles, and 122 DRB1 alleles were detected, and 853 A-B-DRB1 haplotypes, 473 A-B haplotypes, and 551 B-DRB1 haplotypes were statistically inferred. Statistical analysis of three-locus haplotypes showed that A*0207-B*4601-DRB1*0901 (3.06%) was the most predominant. Gene frequencies and haplotypic associations within HLA-A, -B, and -DRB1 loci were determined at a high-resolution (four digit) allelic level and should provide useful information in anthropology, bone marrow donor registry, legal medicine, and disease association studies.
authors.affiliation: The National Laboratory of the Ministry of Health for Forensic Sciences, Xi'an Jiaotong University, Xi'an, China.
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Deng
authors.forename: S-N
authors.initials: SN
authors.lastname: Hu
authors.forename: D-Y
authors.initials: DY
authors.lastname: Wu
authors.forename: S-B
authors.initials: SB
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Zhu
authors.forename: B-F
authors.initials: BF
authors.lastname: Zhu
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000483:Alleles; D002681:China / Q000453:epidemiology; D005787:Gene Frequency; D014644:Genetic Variation; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains