Vaccination of mice against intestinal Trichinella spiralis infections by oral administration of antigens microencapsulated in methacrilic acid copolymers.
BALB/c and NIH mice have been successfully vaccinated against the intestinal nematode Trichinella spiralis by oral administration of crude larval extracts (CLE) and excretory-secretory (ES) products derived from first stage T. spiralis larvae (L1) encapsulated in microcapsules made of copolymers of the metacrylic acid (Eudragit L100). Oral vaccination stimulated the secretion of IFN-gamma and inhibited the secretion of IL-4 in spleen and mesenteric lymph nodes (MLN) of BALB/c mice. In vaccinated mice the proportion of CD4+ cells increased (p<0.05) in Peyer's patches (PP) and decreased (p<0.05) in spleen whereas the proportion of CD19+ cells decreased (p<0.05) in both PP and spleen, with regard to unvaccinated controls. No variation was evident for the proportion of CD8+ cells. Oral vaccination elevated the antigen-specific serum IgG1 and IgA (p<0.05) as well as the antigen-specific IgA response in MLN (p<0.05). It is concluded that this way of vaccination induced a concurrent Th1/ Th2 local and systemic responses that are protective and at the same time they may help balancing the strong Th2 response triggered by helminth infections.
Dea-Ayuela
María A
MA
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, Plaza de Ramón y Cajal s/n, Ciudad Universitaria, 28040 Madrid, Spain.
Rama-Iñiguez
Sara
S
Bolas-Fernández
Francisco
F
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000284:Administration, Oral; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000276:immunology*; D002214:Capsules; D004195:Disease Models, Animal; D005260:Female; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D008198:Lymph Nodes / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000276:immunology; D011109:Polymethacrylic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology; D017160:Trichinella spiralis / Q000276:immunology; D014235:Trichinellosis / Q000276:immunology / Q000517:prevention & control*; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D002214:Capsules; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011109:Polymethacrylic Acids; D014612:Vaccines; C038300:methylmethacrylate-methacrylic acid copolymer; D007371:Interferon-gamma