Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches.
We have sequenced 36,641 expressed sequence tags from laser capture microdissected adult mouse gastric and small intestinal epithelial progenitors, obtaining 4031 and 3324 unique transcripts, respectively. Using Gene Ontology (GO) terms, each data set was compared with cDNA libraries from intact adult stomach and small intestine. Genes in GO categories enriched in progenitors were filtered against genes in GO categories represented in hematopoietic, neural, and embryonic stem cell transcriptomes and mapped onto transcription factor networks, plus canonical signal transduction and metabolic pathways. Wnt/beta-catenin, phosphoinositide-3/Akt kinase, insulin-like growth factor-1, vascular endothelial growth factor, integrin, and gamma-aminobutyric acid receptor signaling cascades, plus glycerolipid, fatty acid, and amino acid metabolic pathways are among those prominently represented in adult gut progenitors. The results reveal shared as well as distinctive features of adult gut stem cells when compared with other stem cell populations.
Giannakis
Marios
M
Center for Genome Sciences, Washington University, St. Louis, Missouri 63108, USA.
Stappenbeck
Thaddeus S
TS
Mills
Jason C
JC
Leip
Douglas G
DG
Lovett
Michael
M
Clifton
Sandra W
SW
Ippolito
Joseph E
JE
Glasscock
Jarret I
JI
Arumugam
Manimozhiyan
M
Brent
Michael R
MR
Gordon
Jeffrey I
JI
D000818:Animals; D019295:Computational Biology; D018076:DNA, Complementary / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism*; D020224:Expressed Sequence Tags; D005753:Gastric Mucosa / Q000378:metabolism*; D015723:Gene Library; D016678:Genome; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism; D007834:Lasers; D051379:Mice; D008954:Models, Biological; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D012984:Software; D013234:Stem Cells / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D018076:DNA, Complementary; D012333:RNA, Messenger; D014157:Transcription Factors