title: Isolation and characterization of another cDNA encoding a chorismate mutase from the phytoparasitic nematode Meloidogyne arenaria.
abstract: A new cDNA, named Ma-cm-2, encoding a chorismate mutase (CM), has been isolated from Meloidogyne arenaria. The full-length cDNA, carrying the trans-spliced SL1 leader sequence, was 753-bp long with an open reading frame of 576 bp. The deduced protein MA-CM-2 including amino-terminal signal peptide shows significant similarity to CMs of Meloidogyne incognita, Meloidogyne javanica, and also bacteria. Secondary structure prediction of MA-CM-2 indicates the presence of the three conserved alpha-helix domains present in the Escherichia coli CMs. Reverse transcription and polymerase chain reaction analysis showed that its transcript abundance is high in the early developmental stages and low in later ones. In situ mRNA hybridization revealed that the transcripts of Ma-cm-2 accumulated specifically in the two subventral oesophageal gland cells of M. arenaria. The widespread existence of CMs in the sedentary endoparasitic nematodes implicates that this enzyme plays an important role in the host-parasite interaction.
authors.affiliation: Department of Plant Pathology, Nanjing Agricultural University, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Long
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Jian Hua
authors.initials: JH
authors.lastname: Xu
authors.forename: Yong Jian
authors.initials: YJ
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002826:Chorismate Mutase / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry*; D018509:DNA, Helminth / Q000737:chemistry; D020224:Expressed Sequence Tags; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D018551:Solanum lycopersicum; D008969:Molecular Sequence Data; D018510:RNA, Helminth / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D014431:Tylenchoidea / Q000201:enzymology* / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018509:DNA, Helminth; D018510:RNA, Helminth; D012333:RNA, Messenger; D002826:Chorismate Mutase