title: Dictyostelium myosin II mechanochemistry promotes active behavior of the cortex on long time scales.
abstract: Cell cortices rearrange dynamically to complete cytokinesis, crawlin response to chemoattractant, build tissues, and make neuronal connections. Highly enriched in the cell cortex, actin, myosin II, and actin crosslinkers facilitate cortical movements. Because cortical behavior is the consequence of nanoscale biochemical events, it is essential to probe the cortex at this level. Here, we use high-resolution laser-based particle tracking to examine how myosin II mechanochemistry and dynacortin-mediated actin crosslinking control cortex dynamics in Dictyostelium. Consistent with its low duty ratio, myosin II does not directly drive active bead motility. Instead, myosin II and dynacortin antagonistically regulate other active processes in the living cortex.
authors.affiliation: Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.
authors.forename: Kristine D
authors.initials: KD
authors.lastname: Girard
authors.forename: Scot C
authors.initials: SC
authors.lastname: Kuo
authors.forename: Douglas N
authors.initials: DN
authors.lastname: Robinson
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002465:Cell Movement; D004023:Dictyostelium / Q000502:physiology*; D024462:Myosin Type II / Q000737:chemistry* / Q000502:physiology*
chemical_list: D024462:Myosin Type II