title: Fibrin glue: an alternative technique for nerve coaptation--Part II. Nerve regeneration and histomorphometric assessment.
abstract: The results of nerve repair with fibrin glue and microsuture were evaluated in rat nerve transection models. Ninety Wistar-Furth rat median nerves were exposed, transected, and repaired in an end-to-end fashion with one of four substances/techniques: 1) human fibrin sealant (Quixil); 2) autologous graft and human fibrin sealant (Quixil); 3) bovine fibrin sealant (Tissucol); and 4) nylon microsuture, epineurial technique. Histologic analyses were performed at 3-, 6-, and 9-month postoperative intervals, and factors evaluated included: presence of inflammatory cells (i.e., macrophages and T cells); number of Schwann cells at the repair site; number of blood vessels; fibrosis; axonal regeneration; and fiber alignment. An additional group underwent histologic analysis at 3 weeks following repair with Quixil. Surgical time of repair was also measured. Nerve repairs performed with fibrin sealants produced less inflammatory response and fibrosis, and better axonal regeneration and fiber alignment than nerve repairs performed with microsuture. In addition, the fibrin sealant techniques were quicker and easier to use. The authors conclude that fibrin sealant represents a good alternative technique to microsuture for peripheral-nerve repair.
authors.affiliation: School of Medicine, Anahuac University, Mexico City, Mexico.
authors.forename: Lorraine
authors.initials: L
authors.lastname: Ornelas
authors.forename: Luis
authors.initials: L
authors.lastname: Padilla
authors.forename: Mauricio
authors.initials: M
authors.lastname: Di Silvio
authors.forename: Paul
authors.initials: P
authors.lastname: Schalch
authors.forename: Sandro
authors.initials: S
authors.lastname: Esperante
authors.forename: Raul López
authors.initials: RL
authors.lastname: Infante
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Bustamante
authors.forename: Pablo
authors.initials: P
authors.lastname: Avalos
authors.forename: Deborah
authors.initials: D
authors.lastname: Varela
authors.forename: Manuel
authors.initials: M
authors.lastname: López
mesh_terms: D000818:Animals; D016009:Chi-Square Distribution; D015718:Fibrin Tissue Adhesive; D008475:Median Nerve / Q000293:injuries / Q000601:surgery* / Q000648:ultrastructure; D008866:Microsurgery; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013536:Suture Techniques
chemical_list: D015718:Fibrin Tissue Adhesive