Mapping of odor-related neuronal activity using a fluorescent derivative of glucose.
Activity labeling was applied to the olfactory systems of the terrestrial slug Limax valentianus using 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG), a fluorescent derivative of glucose. 2-NBDG was incorporated into cultured Limax olfactory interneurons, and this was partially blocked by the presence of a high concentration of glucose in the medium, indicating that a part of the uptake of 2-NBDG is mediated by glucose transporters. Next, in order to map odor-related neuronal activity in the primary olfactory center, tentacular ganglion, we injected 2-NBDG into the body cavities of slugs and exposed them to odors or clean air (control). In the odor-stimulated animals, the cell mass region was strongly stained. The digit-like extensions and the neuropil region were also stained in some animals. The control animals showed no staining. The neurons in the cell mass are thought to be involved in generating oscillating activities in the tentacular ganglion, and their activation may imply modulation of oscillatory activity during odor processing. Our results show that 2-NBDG is useful for mapping neuronal activity in vivo.
Ito
Iori
I
Laboratory of Neurobiophysics, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Watanabe
Satoshi
S
Kirino
Yutaka
Y
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives*; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D005456:Fluorescent Dyes; D017952:Ganglia, Invertebrate / Q000166:cytology; D005944:Glucosamine / Q000031:analogs & derivatives*; D005947:Glucose / Q000378:metabolism; D007395:Interneurons / Q000502:physiology; D008974:Mollusca; D009474:Neurons / Q000502:physiology*; D009812:Odorants; D009833:Olfactory Pathways / Q000166:cytology / Q000502:physiology*
D003470:Culture Media; D005456:Fluorescent Dyes; C045400:6-deoxy-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminoglucose; D009327:4-Chloro-7-nitrobenzofurazan; D005947:Glucose; D005944:Glucosamine