title: Hematopoietic origins of fibroblasts: I. In vivo studies of fibroblasts associated with solid tumors.
abstract: OBJECTIVE
  Recent studies have reported that bone marrow cells can give rise to tissue fibroblasts. However, the bone marrow cell(s) that gives rise to fibroblasts has not yet been identified. In the present study, we tested the hypothesis that tissue fibroblasts are derived from hematopoietic stem cells (HSCs) in vivo.
  
  
  METHODS
  These studies were conducted using mice whose hematopoiesis had been reconstituted by transplantation of a clonal population of cells derived from a single enhanced green fluorescent protein (EGFP)-positive HSC in conjunction with murine tumor models.
  
  
  RESULTS
  When tumors propagated in the transplanted mice were evaluated for the presence of EGFP(+) HSC-derived cells, two prominent populations of EGFP(+) cells were found. The first were determined to be fibroblasts within the tumor stromal capsule, a subset of which expressed type I collagen mRNA and alpha-smooth muscle actin. The second population was a perivascular cell associated with the CD31(+) tumor blood vessels.
  
  
  CONCLUSIONS
  These in vivo findings establish an HSC origin of fibroblasts.
authors.affiliation: Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, USA.
authors.forename: Amanda C
authors.initials: AC
authors.lastname: LaRue
authors.forename: Masahiro
authors.initials: M
authors.lastname: Masuya
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ebihara
authors.forename: Paul A
authors.initials: PA
authors.lastname: Fleming
authors.forename: Richard P
authors.initials: RP
authors.lastname: Visconti
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Minamiguchi
authors.forename: Makio
authors.initials: M
authors.lastname: Ogawa
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Drake
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D002999:Clone Cells; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017154:Stromal Cells / Q000473:pathology; D014184:Transplantation, Homologous; D014407:Tumor Cells, Cultured; D014916:Whole-Body Irradiation
chemical_list: D000199:Actins; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins