title: Angiogenesis facilitated by autologous whole bone marrow stem cell transplantation for Buerger's disease.
abstract: We hypothesized that angiogenesis can be triggered by autologous whole bone marrow stem cell transplantation. Twenty-seven patients (34 lower limbs) with Buerger's disease, who were not candidates for surgical revascularization or radiologic intervention, were enrolled in this study. Six sites of the tibia bone were fenestrated using a 2.5-mm-diameter screw under fluoroscopic guidance. Clinical status and outcome were determined using the "Recommended Standards for Reports." To mobilize endothelial progenitor cells (EPCs) from bone marrow, recombinant human granulocyte colony-stimulating factor (r-HuG-CSF) was injected subcutaneously as a dose of 75 microg, preoperatively. During the follow-up period (19.1 +/- 3.5 months), one limb showed a markedly improved outcome (+3), and 26 limbs showed a moderately improved outcome (+2). Thirteen limbs among 17 limbs with nonhealing ulcers healed. Postoperative angiograms were obtained for 22 limbs. Two limbs showed marked (+3), five limbs moderate (+2), and nine limbs slight (+1) collateral development. However, six limbs showed no collateral development (0). Peripheral blood and bone marrow samples were analyzed for CD34 and CD133 molecules to enumerate potential EPCs, and EPC numbers were found to be increased in peripheral blood and in bone marrow after r-HuG-CSF injection. In conclusion, the transplantation of autologous whole BMCs by fenestration of the tibia bone represents a simple, safe, and effective means of inducing therapeutic angiogenesis in patients with Buerger's disease.
authors.affiliation: Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Kangnamku, Seoul 135-710, Korea. dikim@smc.samsung.co.kr
authors.forename: Dong-Ik
authors.initials: DI
authors.lastname: Kim
authors.forename: Mi-Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Jin-Hyun
authors.initials: JH
authors.lastname: Joh
authors.forename: Sung-Wook
authors.initials: SW
authors.lastname: Shin
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Do
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Moon
authors.forename: Na-Ri
authors.initials: NR
authors.lastname: Kim
authors.forename: Joung-Eun
authors.initials: JE
authors.lastname: Lim
authors.forename: Ae-Kyeong
authors.initials: AK
authors.lastname: Kim
authors.forename: Hyun-Seon
authors.initials: HS
authors.lastname: Eo
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Seung-Hye
authors.initials: SH
authors.lastname: Yang
authors.forename: Chan-Jeoung
authors.initials: CJ
authors.lastname: Park
authors.forename: Jong-Sup
authors.initials: JS
authors.lastname: Shim
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells; D005260:Female; D006801:Humans; D008297:Male; D018919:Neovascularization, Physiologic; D033581:Stem Cell Transplantation; D013919:Thromboangiitis Obliterans / Q000628:therapy*; D014182:Transplantation, Autologous; D016896:Treatment Outcome