title: Determination of platelet-activating factor by reverse phase high-performance liquid chromatography and its application in viral hepatitis.
abstract: OBJECTIVE
  To detect the platelet-activating factor (PAF) and the plasma or serum levels of tumor necrosis factor-alpha (TNF-alpha) malondialdehyde (MDA), endotoxin (ET) and to discuss their significance in various types of viral hepatitis.
  
  
  METHODS
  PAF, TNF-alpha, MDA, and ET levels in 60 controls, 16 cases of acute viral hepatitis, 71 cases of chronic viral hepatitis, 19 cases of severe viral hepatitis were detected by reverse phase high-performance liquid chromatography (rHPLC), bio-assay, ELISA, thiobarbituric acid (TBA), and limulus lysate test (LLT), respectively.
  
  
  RESULTS
  The rHPLC was more sensitive and specific than bio-assay (r = 0.912, P<0.01). The plasma levels of PAF, TNF-alpha, MDA, and ET in patients with viral hepatitis were higher than those in controls (P<0.01).
  
  
  CONCLUSIONS
  rHPLC is more reliable and accurate for the detection of PAF.
authors.affiliation: Department of Infectious Disease, First Affiliated Hospital, Medical School of Zhejiang University, Zhejiang Province, China.
authors.forename: Hong-Cui
authors.initials: HC
authors.lastname: Cao
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
mesh_terms: D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods / Q000706:statistics & numerical data; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000706:statistics & numerical data; D004731:Endotoxins / Q000097:blood; D005260:Female; D006525:Hepatitis, Viral, Human / Q000097:blood*; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D010972:Platelet Activating Factor / Q000032:analysis*; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D004731:Endotoxins; D010972:Platelet Activating Factor; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde