Specificity dependence between serological tests for diagnosing bovine brucellosis in Brucella-free farms showing false positive serological reactions due to Yersinia enterocolitica O:9.
When brucellosis false positive serological reactions happen in cattle, the serial use of pairs of specificity-correlated serological tests (rose bengal, complement fixation, competitive ELISA) results in specificities lower than expected. In this situation, highly specific tests, such as the indirect ELISA used alone, may be more adequate than serial testing.
Mainar-Jaime
Raúl C
RC
Department of Veterinary Microbiology, WCVM, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4. raul.mainar@usask.ca
Muñoz
Pilar M
PM
de Miguel
María J
MJ
Grilló
María J
MJ
Marín
Clara M
CM
Moriyón
Ignacio
I
Blasco
José M
JM
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D002002:Brucella / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary; D005189:False Positive Reactions; D015203:Reproducibility of Results; D012395:Rose Bengal / Q000032:analysis; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000592:standards / Q000662:veterinary*; D015008:Yersinia enterocolitica / Q000276:immunology*
D000942:Antigens, Bacterial; D012395:Rose Bengal