Fluorescent antibody-viability staining and beta-glucuronidase assay as rapid methods for monitoring Escherichia coli viability in coastal marine waters.
A faecal pollution monitoring of coastal Messina waters was performed by comparing three (microscopic, enzyme, and culture) methods. Evidence of Escherichia coli cells (29.99 to 96.79% of the total enteropathogenic serotypes) retaining their viability into the marine environment was shown. beta-Glucuronidase activity rates suggested that living cells were also metabolically active. Heavily polluted sites were detected, where improperly treated urban wastes were discharged. Significant relationships between microscopic and enzymatic data proved both methods to be suitable alternatives to the culture method for E. coli detection, improving environmental quality assessment.
Caruso
G
G
Institute for Coastal Marine Environment IAMC-CNR, Section of Messina, Messina, Italy. gabriella.caruso@iamc.cnr.it
De Pasquale
F
F
Mancuso
M
M
Zampino
D
D
Crisafi
E
E
D001431:Bacteriological Techniques; D001681:Biological Assay; D002470:Cell Survival; D015169:Colony Count, Microbial; D004784:Environmental Monitoring / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D019084:Fluorescent Antibody Technique, Indirect; D005966:Glucuronidase / Q000378:metabolism; D012623:Seawater; D013194:Staining and Labeling; D014871:Water Microbiology
D005966:Glucuronidase