Distribution of chondroitin sulfate in human endometrium.
Sulfated glycosaminoglycan (GAG) composition was characterized in the human endometrium during proliferative and secretory phases of the menstrual cycle. Sulfated GAGs were analyzed in endometrium tissue using metachromatic staining, biochemical analysis including electrophoresis before and after specific enzymatic or chemical degradations, and immunostaining with an antibody against chondroitin sulfate (CS). Our results showed that CS was the main sulfated GAG species detected, accompanied by small amounts of heparan sulfate and dermatan sulfate. CS was distributed overall the connective stroma, around arteriole vessels and glands, and there was no important difference in the immunostaining between the proliferative and secretory endometrium phases. Our findings extend previous observations on the GAG composition in the human endometrium providing new information regarding the tissue distribution and location of endometrial CS.
Nasciutti
Luiz E
LE
Departamento de Histologia e Embriologia, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Cidade Universitária-Ilha do Fundão, 21941-590 Rio de Janeiro, RJ Brazil. nasciutt@ufrj.br
Ferrari
Renato
R
Berardo
Plínio T
PT
Souza
Maísa L S
ML
Takiya
Christina M
CM
Borojevic
Radovan
R
Abrão
Maurício S
MS
Silva
Luiz-Claudio F
LC
D000328:Adult; D002809:Chondroitin Sulfates / Q000378:metabolism*; D004717:Endometrium / Q000033:anatomy & histology* / Q000378:metabolism*; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008597:Menstrual Cycle / Q000378:metabolism; D008875:Middle Aged; D014018:Tissue Distribution
D006025:Glycosaminoglycans; D002809:Chondroitin Sulfates