[Changes in tumor necrosis factor-alpha mRNA expression in peripheral polymorphonuclear leukocyte and tissues during posttraumatic sepsis].
OBJECTIVE
To evaluate the changes in tumor necrosis factor-alpha (TNF-alpha) mRNA expression in peripheral polymorphonuclear leukocyte (PMNs) and tissues after cecal ligation puncture (CLP) in rats.


METHODS
Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect TNF-alpha mRNA expression in peripheral PMNs and tissues.


RESULTS
The TNF-alpha mRNA expression in peripheral PMNs rose gradually after CLP, and it began to decrease after reaching the peak at 48 hours, but it was still higher than normal. The elevation of TNF-alpha mRNA expression was first limited in the regional tissues (P<0.01 at 12 hours after CLP and peaked at 24 hours in the intestine), then it entered the blood circulation later to affect the sensitive organs--lungs and livers (P<0.01 at 24 hours after CLP and peaked at 48 hours in the lung; P<0.05 at 24 hours after CLP and peaked at 48 hours in the liver).


CONCLUSIONS
The rise of TNF-alpha mRNA expression plays an important role in pathogenesis of sepsis.
Xi
Jian-cheng
JC
Department of Orthopaedics, Second Affiliated Hospital of General Hospital of PLA, Beijing 100091, China. jianchengxi309@yahoo.com.cn
Ma
Yuan-zheng
YZ
Zhou
Bao-tong
BT
Lin
Hong-yuan
HY
He
Zhong-jie
ZJ
Zhang
Xing-huo
XH
Fu
Xiao-bing
XB
Sheng
Zhi-yong
ZY
D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000209:etiology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D014409:Tumor Necrosis Factor-alpha