title: G-protein beta3 subunit gene variant (C825T) is unlikely to have a significant influence on serum total cholesterol level in Japanese workers.
abstract: We investigated the association between hypercholesterolemia or serum total cholesterol level and the C825T polymorphism in the G-protein ss3 subunit gene in Japanese workers using multivariate analysis to control possible confounding factors. The study subjects were 1,452 males and 1,169 females selected from 3,834 males and 2,591 females. Hypercholesterolemia was classified as either a total serum cholesterol $5.7 mmol/L (220 mg/dL) or taking lipid-lowering medication. The statistical power of the study was estimated as 99.9% based on total cholesterol levels of previous significant studies in Caucasians and Japanese. The genotype distributions in males with hypercholesterolemia (CC = 78, CT = 154, TT = 78) and females (CC = 60, CT = 137, TT = 52) were not significantly different from normal males (CC = 280, CT = 595, TT = 267) or females (CC = 234, CT = 501, TT = 185). Allele distributions also were not significantly different in either sex. Logistic regression analysis of hypercholesterolemia data and analysis of variance, t-test and multiple regression of serum total cholesterol data demonstrated no significant relationship with the C825T genotype. Despite sufficient statistical power, this study did not demonstrate a significant influence of C825T on hypercholesterolemia or serum total cholesterol level. The targeting of this polymorphism therefore is unlikely to be beneficial in preventing hypercholesterolemia in the general Japanese population.
authors.affiliation: Department of Occupational and Environmental Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan. suwa@faculty.chiba-u.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Suwazono
authors.forename: Etsuko
authors.initials: E
authors.lastname: Kobayashi
authors.forename: Mirei
authors.initials: M
authors.lastname: Uetani
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Miura
authors.forename: Yuko
authors.initials: Y
authors.lastname: Morikawa
authors.forename: Masao
authors.initials: M
authors.lastname: Ishizaki
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Kido
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Koji
authors.initials: K
authors.lastname: Nogawa
mesh_terms: D000483:Alleles; D002784:Cholesterol / Q000097:blood* / Q000235:genetics*; D003430:Cross-Sectional Studies; D005260:Female; D014644:Genetic Variation; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000235:genetics*; D007564:Japan; D008297:Male; D015999:Multivariate Analysis; D020641:Polymorphism, Single Nucleotide
chemical_list: C454420:G-protein beta3 subunit; D002784:Cholesterol; D020962:Heterotrimeric GTP-Binding Proteins