Human adipose-derived stem cells display myogenic potential and perturbed function in hypoxic conditions.
Here, we enriched a human cell population from adipose tissue that exhibited both mesenchymal plasticity, self-renewal capacity, and a cell-surface marker profile indistinguishable from that of bone marrow-derived mesenchymal stem cells. In addition to adipogenic and osteogenic differentiation, these adipose-derived stem cells displayed skeletal myogenic potential when co-cultured with mouse skeletal myocytes in reduced serum conditions. Physical incorporation of stem cells into multinucleated skeletal myotubes was determined by genetic lineage tracing, whereas human-specific antibody staining was employed to demonstrate functional contribution of the stem cells to a myogenic lineage. To investigate the effects of hypoxia, cells were maintained and differentiated at 2% O(2). In contrast with reports on bone marrow-derived stem cells, both osteogenic and adipogenic differentiation were significantly attenuated. In summary, the relative accessibility of adipose-derived mesenchymal stem cells from human donors provides opportunity for molecular investigation of mechanistic dysfunction in disease settings and may introduce new prospects for cell-based therapy.
Lee
Jee-Hyung
JH
Diabetes and Metabolism Disease Area, Novartis Institutes for BioMedical Research, Cambridge, MA 02139, USA.
Kemp
Daniel M
DM
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D000941:Antigens / Q000378:metabolism; D002454:Cell Differentiation; D015687:Cell Hypoxia / Q000502:physiology*; D002460:Cell Line; D019070:Cell Lineage; D018920:Coculture Techniques; D006801:Humans; D051379:Mice; D024510:Muscle Development; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*
D000941:Antigens