title: Formulation in tablets of a cholera whole cells inactivated vaccine candidate.
abstract: Licensed as well as candidate cholera vaccines available at the present requires the dose preparation (included buffer) at the moment of application. The aim of this work was to evaluate the presentation in oral tablets of an inactivated cholera vaccine to avoid that inconveniences during application. We have therefore compared inactivated cultures of Vibrio cholerae with tablets formulation vaccine. We obtained that antigenic activity (ELISA) and immunogenicity in animal model (ELISA and vibriocidal tests) of V. cholerae inactivated cell remained unaltered in the final tablet formulation. The results suggest that the oral tablet formulation could be a useful pharmaceutical form in order to produce a new and affordable cholera vaccine.
authors.affiliation: Finlay Institut, 27 Ave. # 19805, La Lisa, Ciudad de la Habana, A.P. 16017, Cod. 11600, Cuba. atalavera@finlay.edu.cu
authors.forename: A
authors.initials: A
authors.lastname: Talavera
authors.forename: G
authors.initials: G
authors.lastname: Año
authors.forename: Y
authors.initials: Y
authors.lastname: Pino
authors.forename: J
authors.initials: J
authors.lastname: Castaño
authors.forename: E
authors.initials: E
authors.lastname: Uribarri
authors.forename: L
authors.initials: L
authors.lastname: Riverón
authors.forename: S
authors.initials: S
authors.lastname: Gil
authors.forename: S
authors.initials: S
authors.lastname: Fernández
authors.forename: B
authors.initials: B
authors.lastname: Cedré
authors.forename: T
authors.initials: T
authors.lastname: Valmaseda
authors.forename: J L
authors.initials: JL
authors.lastname: Pérez
authors.forename: J F
authors.initials: JF
authors.lastname: Infante
authors.forename: L
authors.initials: L
authors.lastname: García
authors.forename: G
authors.initials: G
authors.lastname: Sierra
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002771:Cholera / Q000517:prevention & control*; D022121:Cholera Vaccines / Q000008:administration & dosage* / Q000276:immunology; D015169:Colony Count, Microbial; D004797:Enzyme-Linked Immunosorbent Assay; D023421:Models, Animal; D011817:Rabbits; D013607:Tablets; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014734:Vibrio cholerae / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D022121:Cholera Vaccines; D013607:Tablets; D015164:Vaccines, Inactivated