title: Chemokine- and adhesion-dependent survival of neutrophils after transmigration through cytokine-stimulated endothelium.
abstract: We examined the fate of neutrophils following transmigration through an endothelial monolayer cultured on "Transwell" membrane filters. Treatment of human umbilical vein endothelial cells (HUVEC) with increasing doses of tumor necrosis factor-alpha increased the efficiency of transmigration and markedly reduced apoptosis among the transmigrated neutrophils in a dose-dependent manner. Apoptosis was also inhibited after transmigration of neutrophils through HUVEC stimulated with interleukin (IL)-1beta but not so effectively after chemotaxis through unstimulated HUVEC driven by IL-8 added below the filter. Inhibition of beta2-integrin binding after transmigration or coating the lower chamber with a nonadhesive polymer (polyhydroxyl-ethyl-methacrylate) abrogated neutrophil survival. Although integrin engagement during migration itself was not essential to inhibit apoptosis, activation of neutrophils through CXC chemokine receptors was necessary. Quite brief exposure to the HUVEC (30-120 min) was effective in reducing subsequent apoptosis, although if coincubation with the HUVEC were prolonged, neutrophil apoptosis was reduced further. Neutralization of granulocyte macrophage-colony stimulating factor inhibited this additional effect. Thus, a complex interplay between migration- and activation-dependent signals and adhesive interaction in tissue may combine to effectively prolong the survival of neutrophils recruited during inflammation.
authors.affiliation: Centre for Cardiovascular Sciences, The Medical School, The University of Birmingham, Birmingham, B15 2TT, UK.
authors.forename: Helen M
authors.initials: HM
authors.lastname: McGettrick
authors.forename: Janet M
authors.initials: JM
authors.lastname: Lord
authors.forename: Ke-Qing
authors.initials: KQ
authors.lastname: Wang
authors.forename: G Ed
authors.initials: GE
authors.lastname: Rainger
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Buckley
authors.forename: Gerard B
authors.initials: GB
authors.lastname: Nash
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001372:Aza Compounds / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D018925:Chemokines / Q000502:physiology*; D002867:Chromones / Q000494:pharmacology; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D009025:Morpholines / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D012016:Reference Values; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000494:pharmacology
chemical_list: C511991:8-(2,6-dichlorophenyl)-10-methyl-3-((4-morpholin-4-ylphenyl)amino)-2,4,10-triazabicyclo(4.4.0)deca-1,3,5,7-tetraen-9-one; D001372:Aza Compounds; D018925:Chemokines; D002867:Chromones; D016207:Cytokines; D007375:Interleukin-1; D009025:Morpholines; D014409:Tumor Necrosis Factor-alpha; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one