title: The major allergen of Alternaria alternata (Alt a 1) is expressed in other members of the Pleosporaceae family.
abstract: There is general consensus regarding the scarce cross-reactivity existing between Alternaria alternata and other allergenic moulds such as Aspergillus fumigatus, Penicillium notatum or Cladosporium herbarum. However, A. alternata has been shown to have a very significant level of allergenic cross-reactivity with other fungi belonging to the Pleosporaceae family. To date, no biological identity or homologies with other proteins have been described for Alt a 1, and it remains unclear whether the major allergen Alt a 1 contributes to the cross-reactivity shown for these moulds. Specific quantification of Alt a 1 in culture filtrates of Stemphylium botryosum, Ulocladium botrytis, Curvularia lunata, Alternaria tenuissima, C. herbarum, Penicillium chrysogenum and Asp. fumigatus, and immunoblotting using culture filtrate extracts from the above-mentioned moulds and rabbit serum anti-recombinant Alt a 1 have shown significant amounts of Alt a 1 in culture filtrates as well as antigenic components ranging from 14 to 20 kDa that strongly react with the specific serum for all taxonomically related species (Pleosporaceae family). No reactions were revealed in culture filtrates of Cladosporium, Penicillium and Aspergillus. These results restrict the cross-reactivity phenomenon due to Alt a 1 to the scope of the taxonomical term of family.
authors.affiliation: Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country, Vitoria, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Sáenz-de-Santamaría
authors.forename: I
authors.initials: I
authors.lastname: Postigo
authors.forename: A
authors.initials: A
authors.lastname: Gutierrez-Rodríguez
authors.forename: G
authors.initials: G
authors.lastname: Cardona
authors.forename: J A
authors.initials: JA
authors.lastname: Guisantes
authors.forename: J
authors.initials: J
authors.lastname: Asturias
authors.forename: J
authors.initials: J
authors.lastname: Martínez
mesh_terms: D000485:Allergens / Q000032:analysis*; D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis*; D052179:Antigens, Plant; D001203:Ascomycota / Q000276:immunology*; D015153:Blotting, Western; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000032:analysis*; D006801:Humans; D008297:Male; D008970:Molecular Weight; D011817:Rabbits
chemical_list: C096258:ALTA1 protein, Alternaria alternata; D000485:Allergens; D000946:Antigens, Fungal; D052179:Antigens, Plant; D003470:Culture Media; D005656:Fungal Proteins