Aging enhances intracerebral hemorrhage-induced brain injury in rats.
Age is an important factor affecting oxidative stress and plasticity after brain injury. The present study investigated the effects of aging on brain injury after intracerebral hemorrhage (ICH). Aging (18-month) and young (3-month) male Sprague-Dawley rats received an intracerebral infusion of 100-microl autologous blood. Age-related changes in brain edema and neurological deficits were examined and heat shock protein 27 (HSP27) and heat shock protein 32 (HSP32) levels were determined by Western blotting. Perihematomal brain swelling was more severe in aged rats compared to young rats at three days after ICH (P < 0.05). The behavioral tests used were forelimb placing test and forelimb use asymmetry test. There were more severe neurological deficits and a slower recovery in aged rats compared to those in young rats after ICH (P < 0.05). In addition, perihematomal HSP27 and HSP32 protein levels were higher (p < 0.05) in aged rats. In conclusion, ICH causes more severe brain swelling and neurological deficits in aged rats. Clarification of the mechanisms of brain injury after ICH in the aging brain should help develop new therapeutic strategies for hemorrhagic brain injury.
Gong
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Department of Neurosurgery, University of Michigan, Ann Arbor, Michigan, 48109-0532, USA.
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Keep
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Hoff
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Hua
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D000375:Aging; D000818:Animals; D001929:Brain Edema / Q000209:etiology / Q000503:physiopathology*; D001930:Brain Injuries / Q000209:etiology / Q000503:physiopathology*; D002543:Cerebral Hemorrhage / Q000150:complications / Q000503:physiopathology*; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D008297:Male; D001523:Mental Disorders / Q000209:etiology / Q000503:physiopathology*; D009363:Neoplasm Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486114:Hspb1 protein, rat; D009363:Neoplasm Proteins; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat