title: Distribution characteristics of ammonia-oxidizing bacteria in the Typha latifolia constructed wetlands using fluorescent in situ hybridization (FISH).
abstract: A molecular biology method, fluorescent in situ hybridization (FISH), in which the pre-treatment was improved in allusion to the media of the constructed wetlands (CW), e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria (AOB) quantity and the relation with oxidation-reduction potential (ORP) in the Typha latifolia constructed wetlands under three different loadings in summer from May to September. Results showed that the quantity of the AOB decreased in the Typha latifolia CW with the increase of vertical depth. However, the AOB quantity was 2-4 times the quantity of the control in the root area. Additionally, ORP in the rhizosphere was found to be higher than other areas, which showed that Typha latifolia CW was in an aerobic state in summer when using simulated non-point sewage at the rural area of Taihu Lake in China and small town combined sewage.
authors.affiliation: School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. ylsjtu@hotmail.com
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Inamori
authors.forename: Ping
authors.initials: P
authors.lastname: Gui
authors.forename: Kai-qin
authors.initials: KQ
authors.lastname: Xu
authors.forename: Hai-nan
authors.initials: HN
authors.lastname: Kong
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Matsumura
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Inamori
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D017404:In Situ Hybridization, Fluorescence; D007564:Japan; D018613:Microscopy, Confocal; D009841:Oligonucleotides; D018517:Plant Roots / Q000382:microbiology*; D012621:Seasons; D012722:Sewage / Q000032:analysis; D012988:Soil Microbiology; D032401:Typhaceae
chemical_list: D009841:Oligonucleotides; D012722:Sewage