CT domain of CCN2/CTGF directly interacts with fibronectin and enhances cell adhesion of chondrocytes through integrin alpha5beta1.
Searching for CCN family protein 2/connective tissue growth factor (CCN2/CTGF) interactive proteins by yeast-two-hybrid screening, we identified fibronectin 1 gene product as a major binding partner of CCN2/CTGF in the chondrosarcoma-derived chondrocytic cell line HCS-2/8. Only the CT domain of CCN2/CTGF bound directly to fibronectin (FN). CCN2/CTGF and its CT domain enhanced the adhesion of HCS-2/8 cells to FN in a dose-dependent manner. The CCN2/CTGF-enhancing effect on cell adhesion to FN was abolished by a blocking antibody against alpha5beta1 integrin (alpha5beta1), but not by one against anti-alphavbeta3 integrin. These findings suggest for the first time that CCN2/CTGF enhances chondrocyte adhesion to FN through direct interaction of its C-terminal CT domain with FN, and that alpha5beta1 is involved in this adhesion.
Hoshijima
Mitsuhiro
M
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 5-1 Shikata-cho, 2-chome, Okayama 700-8525, Japan.
Hattori
Takako
T
Inoue
Miho
M
Araki
Daisuke
D
Hanagata
Hiroshi
H
Miyauchi
Akira
A
Takigawa
Masaharu
M
D001665:Binding Sites; D002448:Cell Adhesion; D002460:Cell Line; D019902:Chondrocytes / Q000166:cytology*; D055513:Connective Tissue Growth Factor; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism*; D039081:Integrin alpha5beta1 / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
C526317:CCN2 protein, human; D005353:Fibronectins; D017874:Immediate-Early Proteins; D039081:Integrin alpha5beta1; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D055513:Connective Tissue Growth Factor