Study on the relationship between level of CD58 expression in peripheral blood mononuclear cell and severity of HBV infection.
BACKGROUND
As one of the intercellular adhesion molecules, CD58 plays important roles in promotion of the adhesion between T cells and target cells, hyperplasia, activation of T cells and natural killer cells, and balance between Th1 and Th2. We studied the relationship between the levels of CD58 expression in peripheral blood mononuclear cells (PBMCs) and severity of HBV infection.


METHODS
The levels of CD58 mRNA in PBMCs were detected using quantitative reverse transcription PCR. The percentage of CD58 positive cells was detected by flow cytometry in patients and healthy controls.


RESULTS
The levels of CD58 mRNA and the percentage of CD58 positive cells in patients infected with HBV were significantly higher than that in the control. Based on severity of HBV infection, the patients were classified into four groups. The expression of CD58 increased significantly in an order from mild chronic, moderate chronic, severe chronic to severe hepatitis groups. The levels of CD58 mRNA and the percentage of CD58 positive cells in PBMCs from patients with HBV infection were both positively correlated with serum levels of ALT and AST.


CONCLUSIONS
The level of CD58 expression is related with the severity of HBV infection and the degree of liver damage.
Xie
Ming
M
Department of Immunology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. iangzhang78@hotmail.com
Wang
Xiang-ling
XL
Ji
Yu-qiang
YQ
Li
Jie
J
Meng
Zhao-jun
ZJ
Shi
Lin
L
Yuan
Yu-kang
YK
D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D018968:CD58 Antigens / Q000235:genetics*; D006509:Hepatitis B / Q000097:blood* / Q000503:physiopathology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis
D018968:CD58 Antigens; D012333:RNA, Messenger; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase