Chromosomal differentiation of populations of Lysapsus limellus limellus, L. l. bolivianus, and of Lysapsus caraya (Hylinae, Hylidae).
Cytogenetic analysis were done on specimens from two populations of Lysapsus limellus limellus, three of L. l. bolivianus and of one of Lysapsus caraya. All animals showed a diploid chromosomal number of 2n=24. The karyotypes of the two L. limellus subspecies were very similar, differing only by the larger amount of telomeric heterochromatin and a small pericentromeric C-band on the short arms of pair 2 in L. l. limellus specimens. The karyotype of L. caraya differed from those of the two L. limellus subspecies in terms of chromosomal morphology, C-banding pattern and location of the main NOR on chromosomes 7 and 6, respectively. The karyotype of the L. l. bolivianus population from Guajará-Mirim/RO differed from those of the other populations of the same subspecies in morphology and heterochromatin pattern of chromosomes 7 and 8. Additional NORs were detected by silver staining and confirmed by FISH in one of the homologues of pairs 1 and 8 in L. l. bolivianus and in pair 7 in L. caraya. These results suggest that a reassessment of the taxonomic status of L. limellus subspecies, especially of the L. l. bolivianus populations, may be necessary.
Busin
Carmen Sílvia
CS
Departamento de Biologia Celular, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, 13083-863 Campinas, SP, Brazil.
Lima
Albertina Pimentel
AP
de Almeida Prado
Cynthia Peralta
CP
Strüssmann
Christine
C
Júnior
Sérgio Siqueira
SS
Recco-Pimentel
Shirlei Maria
SM
D000818:Animals; D001001:Anura / Q000145:classification* / Q000235:genetics*; D002875:Chromosomes / Q000648:ultrastructure*; D003582:Cytogenetics; D004171:Diploidy; D005260:Female; D006570:Heterochromatin / Q000648:ultrastructure; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D012834:Silver; D013045:Species Specificity; D013194:Staining and Labeling
D006570:Heterochromatin; D012834:Silver