Identification of differentially expressed genes in clear cell renal cell carcinoma by analysis of full-length enriched cDNA library.
Renal cell carcinoma (RCC) is the most common malignancy in adult kidney, and accounts for 3% of malignancies worldwide with increasing incidence. Clear cell RCC (ccRCC) is the major type in RCC. Resection by surgery is the main treatment because the response of ccRCC to traditional therapies is very poor. To identify the tumor-associated genes for better understanding the molecular mechanism of ccRCC, the full-length enriched cDNA libraries of ccRCC and normal kidney tissues were constructed by the oligo-capping method. Nucleotide sequences of the cDNA libraries of ccRCC and normal kidney tissues were sequenced. From the sequence analysis of 19,425 and 12,400 clones of ccRCC and normal kidney tissues, 4356 and 3055 genes were identified, respectively. By comparing the gene-expression patterns of ccRCC and normal tissues, the up- or down-regulated genes were identified. Among these identified genes, the differential expression of annexin A2 and argininosuccinate synthetase genes were further confirmed by quantitative real-time PCR and Western blot analysis.
Tang
Sai-Wen
SW
Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
Chang
Wen-Hsin
WH
Chao
Yu-Wei
YW
Lin
Chung-Yei
CY
Chen
Hsiao-Fen
HF
Lai
Yen-Han
YH
Zhan
Bo-Wen
BW
Su
Yih-Ching
YC
Jane
Sen-Wen
SW
Chen
Yu-Chi
YC
Hsu
Chyong-Ing
CI
Lin
Wei-Chou
WC
Wang
Kao-Chung
KC
Lai
Ming-Kuen
MK
Lin
Jung-Yaw
JY
D017306:Annexin A2 / Q000235:genetics; D001124:Argininosuccinate Synthase / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics
D017306:Annexin A2; D009363:Neoplasm Proteins; D001124:Argininosuccinate Synthase