title: Detection of dengue virus serotype 3 by reverse transcription-polymerase chain reaction in Aedes aegypti (Diptera, Culicidae) captured in Manaus, Amazonas.
abstract: The detection of dengue virus serotypes from Aedes aegypti in Manaus, state of Amazonas was carried out using the reverse transcription-polymerase chain reaction technique. Fourteen pools out 82 (17.1%) were positive for DENV3, providing a minimal infection rate of 2.1% of all analyzed infected female specimens of three different areas of the city.
authors.affiliation: Laboratório de Malária e Dengue, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brazil. valeria@cesc.uema.br
authors.forename: Valéria C S
authors.initials: VC
authors.lastname: Pinheiro
authors.forename: Wanderli P
authors.initials: WP
authors.lastname: Tadei
authors.forename: Patrícia M S S
authors.initials: PM
authors.lastname: Barros
authors.forename: Pedro F C
authors.initials: PF
authors.lastname: Vasconcelos
authors.forename: Ana Cecília R
authors.initials: AC
authors.lastname: Cruz
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology / Q000635:transmission / Q000821:virology; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007303:Insect Vectors / Q000821:virology*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping