title: Interaction analysis of the AAA ATPase TAA43 by the bacterial two-hybrid system.
abstract: The TAA43 ATPase of Thermoplasma acidophilum, an archaeal member of the AAA protein family, is known to have an atypical oligomeric state and a nonspecific association with high-molecular-weight protein complexes. We assessed the in vivo binding pattern of TAA43 using the bacterial two-hybrid system. We found 36 positive isolates interacting with TAA43. Our analysis showed that TAA43 interacts preferentially with nonribosomal proteins containing ribosomal domains and regions involved in RNA metabolism.
authors.affiliation: Division of Molecular Biology, Institute for Scientific and Technological Research of San Luis Potosi, San Luis Potosi, Mexico. lsantos@ipicyt.edu.mx
authors.forename: Leticia
authors.initials: L
authors.lastname: Santos
authors.forename: Peter
authors.initials: P
authors.lastname: Zwickl
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D019843:Archaeal Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D013822:Thermoplasma / Q000201:enzymology*; D020798:Two-Hybrid System Techniques
chemical_list: D019843:Archaeal Proteins; D011994:Recombinant Proteins; D000251:Adenosine Triphosphatases