HEN1 recognizes 21-24 nt small RNA duplexes and deposits a methyl group onto the 2' OH of the 3' terminal nucleotide.
microRNAs (miRNAs) and small interfering RNAs (siRNAs) in plants bear a methyl group on the ribose of the 3' terminal nucleotide. We showed previously that the methylation of miRNAs and siRNAs requires the protein HEN1 in vivo and that purified HEN1 protein methylates miRNA/miRNA* duplexes in vitro. In this study, we show that HEN1 methylates both miRNA/miRNA* and siRNA/siRNA* duplexes in vitro with a preference for 21-24 nt RNA duplexes with 2 nt overhangs. We also demonstrate that HEN1 deposits the methyl group on to the 2' OH of the 3' terminal nucleotide. Among various modifications that can occur on the ribose of the terminal nucleotide, such as 2'-deoxy, 3'-deoxy, 2'-O-methyl and 3'-O-methyl, only 2'-O-methyl on a small RNA inhibits the activity of yeast poly(A) polymerase (PAP). These findings indicate that HEN1 specifically methylates miRNAs and siRNAs and implicate the importance of the 2'-O-methyl group in the biology of RNA silencing.
Yang
Zhiyong
Z
Department of Botany and Plant Sciences, University of California Riverside, Riverside, CA 92521, USA.
Ebright
Yon W
YW
Yu
Bin
B
Chen
Xuemei
X
D029968:Escherichia coli Proteins / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D009711:Nucleotides / Q000737:chemistry; D011062:Polynucleotide Adenylyltransferase / Q000737:chemistry; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000378:metabolism; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity; D015003:Yeasts / Q000201:enzymology
D029968:Escherichia coli Proteins; D035683:MicroRNAs; D009711:Nucleotides; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; C507832:HEN1 protein , Escherichia coli; D008780:Methyltransferases; D011062:Polynucleotide Adenylyltransferase