title: Expression of oxidative stress-related molecules in circulating leukocytes and urine in patients with chronic viral hepatitis.
abstract: OBJECTIVE
  Oxidative stress plays a role in pathogenesis of chronic viral hepatitis. Expression of oxidative stress-related molecules remains to be clarified.
  
  
  METHODS
  4-hydroxy-2-nonenal (4-HNE), 4-hydroxy-2-hexenal (4-HHE), catalase, superoxide dismutase-1 (SOD-1), glutathione peroxidase-1, thioredoxin (TRX) in leukocytes and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) were examined in 164 persons, including 130 chronic viral hepatitis patients and 34 normal individuals, by Western blot analysis and enzyme-linked immunosorbent assay, respectively. Hepatic expression of these proteins was immunohistochemically examined in 12 patients with chronic viral hepatitis, compared with three persons without liver damage.
  
  
  RESULTS
  The 4-HNE/beta-actin ratios in chronic viral hepatitis were significantly higher than those in normal individuals (P<0.01), and were significantly correlated with asparate aminotransferase (AST) and alanine aminotransferase (ALT) (P<0.01, each). The catalase/beta-actin and SOD-1/beta-actin ratios in chronic viral hepatitis were higher than those in normal individuals, and were significantly correlated with 4-HNE/beta-actin ratios (P<0.01, each). Hepatic expression of 4-HNE, 4-HHE, catalase, SOD-1 and TRX in chronic viral hepatitis was higher than that without liver damage. Urinary excretion of 8-OHdG was not changed in chronic viral hepatitis.
  
  
  CONCLUSIONS
  The results of the present study suggest that expression of oxidative stress-related molecules in leukocytes is upregulated in relation to serum aminotransferase levels.
authors.affiliation: Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, Yonago, Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Saeki
authors.forename: Miho
authors.initials: M
authors.lastname: Ichiba
authors.forename: Naotada
authors.initials: N
authors.lastname: Tanabe
authors.forename: Masaru
authors.initials: M
authors.lastname: Ueki
authors.forename: Kinya
authors.initials: K
authors.lastname: Okamoto
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Matsunaga
authors.forename: Keiko
authors.initials: K
authors.lastname: Hosho
authors.forename: Takamasa
authors.initials: T
authors.lastname: Kanbe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kurimasa
authors.forename: Sadako
authors.initials: S
authors.lastname: Yamada
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Hirooka
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hisatome
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kishimoto
authors.forename: Takeaki
authors.initials: T
authors.lastname: Suou
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Murawaki
authors.forename: Hironaka
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Junji
authors.initials: J
authors.lastname: Yodoi
authors.forename: Goshi
authors.initials: G
authors.lastname: Shiota
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood; D000447:Aldehydes / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood; D015153:Blotting, Western; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D004798:Enzymes; D005260:Female; D006525:Hepatitis, Viral, Human / Q000097:blood* / Q000473:pathology / Q000652:urine*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D013879:Thioredoxins / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000447:Aldehydes; D004798:Enzymes; C045934:4-hydroxy-2-hexenal; D013879:Thioredoxins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003849:Deoxyguanosine; C027576:4-hydroxy-2-nonenal