Recombinant human erythropoietin produced in milk of transgenic pigs.
We have developed a line of transgenic swine harboring recombinant human erythropoietin through microinjection into fertilized one cell pig zygotes. Milk from generations F1 and F2 transgenic females was analyzed, and hEPO was detected in milk from all lactating females at concentrations of approximately 877.9+/-92.8 IU/1 ml. The amino acid sequence of rhEPO protein in the transgenic pig milk matched that of commercial rhEPO produced from cultured animal cells. In addition, an F-36 cell line, which proliferates in the presence of hEPO or commercial EPO, was induced to synthesize erythroid by extracts from tg sow milk. This study provides evidence that production of purified rhEPO from transgenic pig milk is a potentially valuable technology, and can be used as a cost-effective alternative in clinical applications as well as providing other clinical advantages.
Park
Jin-Ki
JK
Animal Biotechnology Division, National Livestock Research Institute, Suwon 441-706, Republic of Korea.
Lee
Yun-Keun
YK
Lee
Poongyeon
P
Chung
Hak-Jae
HJ
Kim
Sungwoo
S
Lee
Hyun-Gi
HG
Seo
Myung-Kyu
MK
Han
Joo-Hee
JH
Park
Chun-Gyu
CG
Kim
Hun-Taek
HT
Kim
Yong-Kook
YK
Min
Kwan-Sik
KS
Kim
Jin-Hoi
JH
Lee
Hoon-Taek
HT
Chang
Won-Kyong
WK
D000818:Animals; D030801:Animals, Genetically Modified; D001854:Bone Marrow Cells / Q000166:cytology; D002460:Cell Line; D049109:Cell Proliferation; D004921:Erythropoietin / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008321:Mammary Glands, Animal / Q000378:metabolism; D008892:Milk / Q000378:metabolism*; D011994:Recombinant Proteins; D034421:Sus scrofa / Q000235:genetics*
D011994:Recombinant Proteins; D004921:Erythropoietin