Effects of peripubertal exposure to triphenyltin on female sexual development of the rat.
Triphenyltin (TPT) belongs to the group of organotin compounds which have been shown to affect reproduction in mammals. It is used as a fungicide and antifouling agent and the main source of human exposure is via food. We studied the effects of 2 or 6 mg TPT/kg bw on female sexual development using a modification of the Rodent 20-Day Thyroid/Pubertal Female Assay. Moreover, the effect of TPT before the onset of puberty was investigated. Beginning at postnatal day (PND) 23 female Wistar rats were treated per gavage until either PND 33 or the first estrus after PND 53. A delay in the completion of vaginal opening (VO) was observed in the 6 mg TPT group, while the 2mg TPT group showed advanced VO. Significantly increased ovarian weights were observed in both treatment groups. Steroid hormone levels and ovarian aromatase activity were affected after exposure to 6 mg TPT/kg bw, while treatment with 2mg TPT/kg bw resulted in minor changes of these endpoints. We conclude that peripubertal exposure to 6 mg TPT/kg bw, and to a lesser extent to 2mg TPT/kg bw, affects female sexual development.
Grote
Konstanze
K
Inst. of Clinical Pharmacology and Toxicology, Charité University Medical School, Campus Benjamin Franklin, 14195 Berlin, Germany.
Andrade
Anderson J M
AJ
Grande
Simone Wichert
SW
Kuriyama
Sergio N
SN
Talsness
Chris E
CE
Appel
Klaus E
KE
Chahoud
Ibrahim
I
D000818:Animals; D001141:Aromatase / Q000378:metabolism; D001681:Biological Assay; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D052244:Endocrine Disruptors / Q000633:toxicity; D004958:Estradiol / Q000097:blood; D005260:Female; D005659:Fungicides, Industrial / Q000633:toxicity*; D009929:Organ Size; D009947:Organotin Compounds / Q000633:toxicity*; D010053:Ovary / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D011374:Progesterone / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D046468:Sexual Development / Q000187:drug effects*; D014621:Vagina / Q000187:drug effects / Q000254:growth & development
D052244:Endocrine Disruptors; D005659:Fungicides, Industrial; D009947:Organotin Compounds; D011374:Progesterone; D004958:Estradiol; C030665:triphenyltin; D001141:Aromatase