title: An Agrobacterium-transformed cell culture from the monocot Asparagus officinalis.
abstract: Cultured stem fragments from the monocotyledonous plant Asparagus officinalis infected by the oncogenic bacterium Agrobacterium tumefaciens developed tumorous proliferations. This tissue was propagated in vitro on hormone-free culture medium. The T-DNA-encoded markers nopaline and agrocinopine were unambiguously detected in these tissues. The data demonstrate that stable T-DNA transfer as well as expression of T-DNA genes is possible in at least some monocotyledonous plants. This opens new possibilities for plant genetic engineering using the Ti plasmid as a gene vector.
authors.affiliation: Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, B-1640 St.-Genesius-Rode,FRG.
authors.forename: J P
authors.initials: JP
authors.lastname: Hernalsteens
authors.forename: L
authors.initials: L
authors.lastname: Thia-Toong
authors.forename: J
authors.initials: J
authors.lastname: Schell
authors.forename: M
authors.initials: M
authors.lastname: Van Montagu