Serum levels of 8-isoprostane, a marker of oxidative stress, are elevated in patients with systemic sclerosis.
OBJECTIVE
To determine serum levels and clinical correlation of 8-isoprostane, which is produced in vivo through free radical-catalysed peroxidation of arachidonic acid and reflects oxidative stress, in patients with systemic sclerosis (SSc).


METHODS
Serum 8-isoprostane levels from 32 patients with diffuse cutaneous SSc (dSSc) and 25 patients with limited cutaneous SSc (lSSc) were examined by enzyme-linked immunosorbent assay.


RESULTS
Serum 8-isoprostane levels were elevated in dSSc and lSSc patients by 75-fold compared with normal controls (n=32). Serum 8-isoprostane levels correlated negatively with pulmonary function, such as percentage vital capacity and diffusion capacity for carbon monoxide, and correlated positively with renal vascular damage determined by colour flow Doppler ultrasonography. Serum 8-isoprostane levels also correlated positively with serum levels of IgG and anti-agalactosyl IgG autoantibody.


CONCLUSIONS
Increased 8-isoprostane levels correlated with the severity of pulmonary fibrosis, the extent of renal vascular damage and immunological abnormalities in SSc, suggesting that enhanced oxidative stress is related to the development of SSc.
Ogawa
F
F
Department of Dermatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
Shimizu
K
K
Muroi
E
E
Hara
T
T
Hasegawa
M
M
Takehara
K
K
Sato
S
S
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D002248:Carbon Monoxide; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011653:Pulmonary Diffusing Capacity; D012079:Renal Circulation; D045743:Scleroderma, Diffuse / Q000097:blood / Q000503:physiopathology; D045745:Scleroderma, Limited / Q000097:blood / Q000503:physiopathology; D012595:Scleroderma, Systemic / Q000097:blood* / Q000503:physiopathology; D014655:Vascular Resistance; D014797:Vital Capacity
D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D002248:Carbon Monoxide; D015237:Dinoprost