Identification of RASSF8 as a candidate lung tumor suppressor gene.
The RASSF8 gene, which maps close to the KRAS2 gene, contains a RAS-associated domain and encodes a protein that is evolutionarily conserved from fish to humans. Analysis of the RASSF8 transcript revealed a complex expression pattern of 5'-UTR mRNA isoforms in normal lung and in lung adenocarcinomas (ADCAs), with no apparent differences. However, RASSF8 gene transcript levels were approximately seven-fold-lower in lung ADCAs as compared to normal lung tissue. Expression of RASSF8 protein by transfected lung cancer cells led to inhibition of anchorage-independent growth in soft agar in A549 cells and reduction of clonogenic activity in NCI-H520 cells. These results raise the possibility protein encoded by RASSF8 is a novel tumor suppressor for lung cancer.
Falvella
F S
FS
Department of Experimental Oncology and Laboratories, Istituto Nazionale Tumori, Milan, Italy.
Manenti
G
G
Spinola
M
M
Pignatiello
C
C
Conti
B
B
Pastorino
U
U
Dragani
T A
TA
D020121:5' Untranslated Regions; D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D001483:Base Sequence; D002448:Cell Adhesion / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D017931:DNA Primers; D015536:Down-Regulation; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D020121:5' Untranslated Regions; D017931:DNA Primers; C512680:RASSF8 protein, human; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins