[The effect of polyester vascular prostheses on synthesis TNF-alpha, IFN-gamma and NO by human peripheral blood leukocytes--in vitro].
Vascular prostheses produced on the basis of polyester are today commonly used in vascular surgery. To improve their biofuncionality special technologies are used, among them double knit and hydrophile feature enrichment. These modifications could cause the local activation of leukocytes to produce the mediators of inflammatory reaction, which in turn leads to hyperplasia of endothelium and other dangerous complications. In our study we used two kinds of polyester prostheses: double velour knitted hydrophilic Dallon H and standard double velour knitted prosthesis Dallon as control. The aim of this work was to compare in vitro the levels of cytokines TNF-alpha, interferon (IFN) and nitric oxide (NO) found in the supernatants of human blood leukocyte cultures after stimulation by both these above-mentioned vascular prostheses materials which are designed for use in direct blood contact. Tested implant materials Dallon H had no influence on synthesis of production of IFN, TNF-alpha and NO by human blood leukocytes. These results allow to made the initial selection of biomaterials before their in vivo evaluation.
Zywicka
Bogusława
B
Zakład Chirurgii Eksperymentalnej i Badania Biomateriałów, Akademia Medyczna we Wrocławiu.
Czarny
Anna
A
Zaczyńska
Ewa
E
Pielka
Stanisław
S
Solski
Leszek
L
D001672:Biocompatible Materials / Q000633:toxicity*; D001807:Blood Vessel Prosthesis; D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D011091:Polyesters / Q000145:classification / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D001672:Biocompatible Materials; D011091:Polyesters; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007371:Interferon-gamma