title: Functional genomics reveals genes involved in protein secretion and Golgi organization.
abstract: Yeast genetics and in vitro biochemical analysis have identified numerous genes involved in protein secretion. As compared with yeast, however, the metazoan secretory pathway is more complex and many mechanisms that regulate organization of the Golgi apparatus remain poorly characterized. We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. We then classified the genes on the basis of the effect of their depletion on organization of the Golgi membranes. Here we show that depletion of class A genes redistributes Golgi membranes into the endoplasmic reticulum, depletion of class B genes leads to Golgi fragmentation, depletion of class C genes leads to aggregation of Golgi membranes, and depletion of class D genes causes no obvious change. Of the 20 new gene products characterized so far, several localize to the Golgi membranes and the endoplasmic reticulum.
authors.affiliation: Cell and Developmental Biology Department, University of California San Diego, La Jolla, California 92093-0634, USA.
authors.forename: Frederic
authors.initials: F
authors.lastname: Bard
authors.forename: Laetitia
authors.initials: L
authors.lastname: Casano
authors.forename: Arrate
authors.initials: A
authors.lastname: Mallabiabarrena
authors.forename: Erin
authors.initials: E
authors.lastname: Wallace
authors.forename: Kota
authors.initials: K
authors.lastname: Saito
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kitayama
authors.forename: Gianni
authors.initials: G
authors.lastname: Guizzunti
authors.forename: Yue
authors.initials: Y
authors.lastname: Hu
authors.forename: Franz
authors.initials: F
authors.lastname: Wendler
authors.forename: Ramanuj
authors.initials: R
authors.lastname: Dasgupta
authors.forename: Norbert
authors.initials: N
authors.lastname: Perrimon
authors.forename: Vivek
authors.initials: V
authors.lastname: Malhotra
mesh_terms: D000818:Animals; D002460:Cell Line; D004330:Drosophila / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D017344:Genes, Insect / Q000235:genetics; D017930:Genes, Reporter; D023281:Genomics; D006056:Golgi Apparatus / Q000235:genetics* / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000235:genetics / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology; D034622:RNA Interference
chemical_list: D029721:Drosophila Proteins; D021382:Protein Sorting Signals; D006735:Horseradish Peroxidase