title: Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium.
abstract: The 35S transcript promoter of cauliflower mosaic virus directs the synthesis of an abundant RNA in infected leaf tissue that is both a template for reverse transcription during virus replication and also a polycistronic mRNA. The 35S promoter was isolated and linked to a cDNA of the 3' end of tobacco mosaic virus that encodes the coat protein of this single-stranded RNA virus. After transformation of this gene construction into tobacco plants using a disarmed Agrobacterium binary vector, viral coat protein synthesis was detected in transformed leaf tissue. A chimaeric 35S coat protein RNA was detected in transformed callus.
authors.affiliation: Plant Breeding Institute, Maris Lane, Trumpington, Cambridge CB2 2LQ, UK.
authors.forename: M W
authors.initials: MW
authors.lastname: Bevan
authors.forename: S E
authors.initials: SE
authors.lastname: Mason
authors.forename: P
authors.initials: P
authors.lastname: Goelet