title: Influence of the regulatory protein RsmA on cellular functions in Pseudomonas aeruginosa PAO1, as revealed by transcriptome analysis.
abstract: RsmA is a posttranscriptional regulatory protein in Pseudomonas aeruginosa that works in tandem with a small non-coding regulatory RNA molecule, RsmB (RsmZ), to regulate the expression of several virulence-related genes, including the N-acyl-homoserine lactone synthase genes lasI and rhlI, and the hydrogen cyanide and rhamnolipid biosynthetic operons. Although these targets of direct RsmA regulation have been identified, the full impact of RsmA on cellular activities is not as yet understood. To address this issue the transcriptome profiles of P. aeruginosa PAO1 and an isogenic rsmA mutant were compared. Loss of RsmA altered the expression of genes involved in a variety of pathways and systems important for virulence, including iron acquisition, biosynthesis of the Pseudomonas quinolone signal (PQS), the formation of multidrug efflux pumps, and motility. Not all of these effects can be explained through the established regulatory roles of RsmA. This study thus provides both a first step towards the identification of further genes under RsmA posttranscriptional control in P. aeruginosa and a fuller understanding of the broader impact of RsmA on cellular functions.
authors.affiliation: BIOMERIT Research Centre, Department of Microbiology, National University of Ireland, Cork, Ireland.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Burrowes
authors.affiliation: BIOMERIT Research Centre, Department of Microbiology, National University of Ireland, Cork, Ireland.
authors.forename: Christine
authors.initials: C
authors.lastname: Baysse
authors.affiliation: BIOMERIT Research Centre, Department of Microbiology, National University of Ireland, Cork, Ireland.
authors.forename: Claire
authors.initials: C
authors.lastname: Adams
authors.affiliation: BIOMERIT Research Centre, Department of Microbiology, National University of Ireland, Cork, Ireland.
authors.forename: Fergal
authors.initials: F
authors.lastname: O'Gara
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D020543:Proteome / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D020543:Proteome; C088386:homoserine lactone; D015107:4-Butyrolactone