High-performance liquid chromatography method to measure alpha- and gamma-tocopherol in leaves, flowers and fresh beans from Moringa oleifera.
A high-performance liquid chromatography method for the microscale determination of alpha- and gamma-tocopherol in leaves, flowers and fresh beans from Moringa oleifera is reported. The method includes microscale saponification and extraction with n-hexane. Optimized conditions for reversed-phase HPLC with UV detection were as follows: column, 25 cm x 0.46 cm, Exil ODS 5-microm; column temperature, 25 degrees C; mobile phase, a 20:80 (v/v) mixture of methanol:acetonitrile; flow rate, 1.0 ml/min. With these conditions, method precision (relative standard deviation) was 5.6% for alpha-tocopherol and 4.9% for gamma-tocopherol. We used this method to measure alpha- and gamma-tocopherol in samples from M. oleifera as part of nutritional studies in edible plants cultivated in the Northwest México.
Sánchez-Machado
D I
DI
Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, P.O. Box 541, Antonio Caso s/n, Col. Villa ITSON, 85139 Cd. Obregón, Sonora, Mexico.
López-Cervantes
J
J
Vázquez
N J Ríos
NJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D029588:Moringa oleifera / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D024502:alpha-Tocopherol / Q000032:analysis*; D024504:gamma-Tocopherol / Q000032:analysis*
D024504:gamma-Tocopherol; D024502:alpha-Tocopherol