Lesion of the caudal ventrolateral medulla prevents the induction of hypertension by adenosine receptor blockade in rats.
The continuous infusion for 7 days of the adenosine receptor antagonist 1,3-dipropyl-8-sulfophenylxanthine (DPSPX) causes a sustained hypertension in rats, with an enhancement of sympathetic neurotransmission and activation of the renin-angiotensin system. We studied the involvement of the caudal ventrolateral medulla in the establishment of this hypertensive model by evaluating the effect of local lesioning in blood pressure (BP). Male adult Wistar rats received stereotaxic injections of 0.3 mul of saline or quinolinic acid (QA; 180 mM) in the caudal ventrolateral medulla followed by abdominal implant of minipump for infusion of saline or DPSPX (90 microg(-1) kg(-1) h(-1)). BP was measured in conscious animals every 2 days for 12 days. The sustained increase of BP (22.1 mm Hg; P < 0.001) detected in rats infused with DPSPX was reverted (6.7 mm Hg; P > 0.05) from day six onwards in animals with lesion of the lateralmost part of caudal ventrolateral medulla (VLMlat). The present results suggest that the development of hypertension induced by adenosine receptor antagonist involves the participation of the VLMlat. They further add new data as to the functional complexity of this medullary area involved in a variety of functions such as cardiovascular, respiratory, motor and pain control.
Pinho
Dora
D
Institute of Pharmacology and Therapeutics, Faculty of Medicine of Porto and IBMC, University of Porto, Alameda Prof. Hernani Monteiro, 4200-319 Porto, Portugal.
Sousa
Teresa
T
Morato
Manuela
M
Tavares
Isaura
I
Albino-Teixeira
António
A
D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D006973:Hypertension / Q000139:chemically induced* / Q000517:prevention & control*; D008297:Male; D008526:Medulla Oblongata / Q000293:injuries / Q000473:pathology / Q000503:physiopathology; D058915:Purinergic P1 Receptor Antagonists; D017378:Quinolinic Acid / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014970:Xanthines
D058915:Purinergic P1 Receptor Antagonists; D014970:Xanthines; C053896:1,3-dipropyl-8-(4-sulfophenyl)xanthine; D017378:Quinolinic Acid