Sensitivity and specificity improvement of an ion sensitive field effect transistors-based biosensor for potato glycoalkaloids detection.
Butyryl cholinesterase of different origin along with variations of the time of enzyme immobilization on the potentiometric transducer surface is offered to control the ion sensitive field effect transistor (ISFET)-based biosensor sensitivity. Because butyryl cholinesterase has been already used to develop the sensors for heavy metals, organophosphorus/carbamate pesticides, and steroidal glycoalkaloids analysis, the present study has been focused on the investigation and adjustment of the ISFET-based biosensor specificity exclusively to the glycoalkaloids. Utilization of ethylendiaminetetracetate (a complexon of heavy metal ions) and phosphotriesterase (a highly efficient catalyst for the hydrolysis of organophosphorus compounds) enabled the highly specific determination of glycoalkaloids at the background of lead and mercury (up to 500 microM of ions concentration) and paraoxon (up to 100 microM of pesticide concentration). The developed biosensor has been validated for glycoalkaloids detection in potato varieties cultivated in Ukraine, and the results obtained are compared to those measured by the methods of HPLC and TLC.
Korpan
Yaroslav I
YI
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., UA-03143, Kyiv-143, Ukraine. korpan@imbg.org.au
Raushel
Frank M
FM
Nazarenko
Elena A
EA
Soldatkin
Alexey P
AP
Jaffrezic-Renault
Nicole
N
Martelet
Claude
C
D000470:Alkaloids / Q000032:analysis; D015374:Biosensing Techniques; D002091:Butyrylcholinesterase; D004800:Enzymes, Immobilized; D017736:Ion-Selective Electrodes; D019216:Metals, Heavy / Q000032:analysis; D010575:Pesticides / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D011198:Solanum tuberosum / Q000737:chemistry*; D014173:Transistors, Electronic; D014455:Ukraine
D000470:Alkaloids; D004800:Enzymes, Immobilized; D019216:Metals, Heavy; D010575:Pesticides; D002091:Butyrylcholinesterase