mRNA-based parallel detection of active methanotroph populations by use of a diagnostic microarray.
A method was developed for the mRNA-based application of microbial diagnostic microarrays to detect active microbial populations. DNA- and mRNA-based analyses of environmental samples were compared and confirmed via quantitative PCR. Results indicated that mRNA-based microarray analyses may provide additional information on the composition and functioning of microbial communities.
Bodrossy
Levente
L
Department of Bioresources, ARC Seibersdorf Research GmbH, A-2444 Seibersdorf, Austria. levente.bodrossy@arcs.ac.at
Stralis-Pavese
Nancy
N
Konrad-Köszler
Marianne
M
Weilharter
Alexandra
A
Reichenauer
Thomas G
TG
Schöfer
David
D
Sessitsch
Angela
A
D017753:Ecosystem; D004783:Environmental Microbiology; D005798:Genes, Bacterial; D008697:Methane / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D020560:Proteobacteria / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*
D012333:RNA, Messenger; D008697:Methane