Protein kinase CK2 phosphorylates the Nef protein from a neurovirulent simian immunodeficiency virus.
The Nef protein of Human Immunodeficiency Virus (HIV) and Simian Immunodeficiency Virus (SIV) is a pluripotent accessory protein that plays a critical role in disease progression. One analogous characteristic of Nef proteins from SIV and HIV is the ability to associate with cellular kinases. We have previously reported that the Nef protein from a macrophage-tropic neurovirulent SIV clone, SIV/17E-Fr, is associated with an unknown kinase activity that is distinct from the p21-associated kinase that interacts with SIVmac239 Nef. Using site-directed mutagenesis and kinase-specific inhibitors, we have identified this kinase as the ubiquitous serine/threonine kinase, protein kinase CK2.
Caples
Matthew J
MJ
Department of Comparative Medicine, Johns Hopkins University School of Medicine, 733 N. Broadway St., Broadway Research Building 831, Baltimore, MD 21205, USA.
Clements
Janice E
JE
Barber
Sheila A
SA
D019943:Amino Acid Substitution; D047390:Casein Kinase II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015693:Gene Products, nef / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D010766:Phosphorylation; D011494:Protein Kinases; D011994:Recombinant Proteins / Q000378:metabolism; D015302:Simian Immunodeficiency Virus / Q000737:chemistry*
D004791:Enzyme Inhibitors; D015693:Gene Products, nef; D011994:Recombinant Proteins; D011494:Protein Kinases; D047390:Casein Kinase II