Comparison of soxhlet, ultrasound-assisted and pressurized liquid extraction of terpenes, fatty acids and Vitamin E from Piper gaudichaudianum Kunth.
This paper describes a comparative study of extraction methods of terpenes (terpenic alcohols and phytosterols), fatty acids and Vitamin E from leaves of Piper gaudichaudianum Kunth. The analysis of extracts was done by gas chromatography with mass spectrometric detection. The identification and quantification was made by co-injections of the extract with certified standards. The use of pressurized liquid extraction (PLE; Dionex trade name: ASE, for accelerated solvent extraction) decrease significantly the total time of extraction, the amount of solvent and the manipulation of sample and solvents in comparison with soxhlet (SE) and ultrasound-assisted (USE). In addition, PLE was more effective for the extractions of terpenes (terpenic alcohols and phytosterols), fatty acids and Vitamin E.
Péres
Valéria Flores
VF
Laboratório de Genética Toxicológica, ULBRA, Canoas, RS-Brazil; Universidad de La Habana, Cuba.
Saffi
Jenifer
J
Melecchi
Maria Inês S
MI
Abad
Fernanda C
FC
de Assis Jacques
Rosângela
R
Martinez
Migdalia M
MM
Oliveira
Eniz Conceição
EC
Caramão
Elina B
EB
D005591:Chemical Fractionation / Q000379:methods*; D005227:Fatty Acids / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D031701:Piper / Q000737:chemistry*; D011312:Pressure; D013729:Terpenes / Q000302:isolation & purification*; D014465:Ultrasonics; D014810:Vitamin E / Q000302:isolation & purification*
D005227:Fatty Acids; D013729:Terpenes; D014810:Vitamin E