Modulation of MCP-1 and iNOS by 50-Hz sinusoidal electromagnetic field.
The purpose of this study was to investigate whether overnight exposure to 1 mT-50 Hz extremely low-frequency sinusoidal electromagnetic field (EMF) affects the expression and production of inducible nitric oxide synthase (iNOS) and monocyte chemotactic protein-1 (MCP-1) in human monocytes. RT-PCR and Western blot analysis demonstrate that EMF exposure affects the expression of iNOS and MCP-1 in cultured human mononuclear cells at the mRNA level and protein synthesis. Interestingly, the effects of EMF exposure clearly differed with respect to the potentiation and inhibition of iNOS and MCP-1 expression. Whereas iNOS was down-regulated both at the mRNA level and at the protein level, MCP-1 was up-regulated. These results provide helpful information regarding the EMF-mediated modulation of the inflammatory response in vivo. However, additional studies are necessary to demonstrate that EMF acts as a nonpharmacological inhibitor of NO and inducer of MCP-1 in some diseases where the balance of MCP-1 and NO may be important.
Reale
Marcella
M
Department of Oncology and Neuroscience, Universita degli Studi G.D'Annunzio, Chieti, Italy. mreale@unich.it
De Lutiis
Maria Anna
MA
Patruno
Antonia
A
Speranza
Lorenza
L
Felaco
Mario
M
Grilli
Alfredo
A
Macrì
Maria Antonietta
MA
Comani
Silvia
S
Conti
Pio
P
Di Luzio
Silvano
S
D018932:Chemokine CCL2 / Q000235:genetics / Q000528:radiation effects*; D004574:Electromagnetic Fields; D005260:Female; D015870:Gene Expression / Q000528:radiation effects; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects*; D016328:NF-kappa B / Q000378:metabolism / Q000528:radiation effects; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D014176:Protein Biosynthesis / Q000528:radiation effects; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018932:Chemokine CCL2; D008070:Lipopolysaccharides; D016328:NF-kappa B; D052247:Nitric Oxide Synthase Type II