title: Molecular classification of Crohn's disease and ulcerative colitis patients using transcriptional profiles in peripheral blood mononuclear cells.
abstract: Ulcerative colitis (UC) and Crohn's disease (CD) are common inflammatory bowel diseases producing intestinal inflammation and tissue damage. Although emerging evidence suggests these diseases are distinct, approximately 10% of patients remain classified as indeterminate inflammatory bowel disease even after invasive colonoscopy intended for diagnosis. A molecular diagnostic assay using a clinically accessible tissue would greatly assist in the classification of these diseases. In the present study we assessed transcriptional profiles in peripheral blood mononuclear cells from 42 healthy individuals, 59 CD patients, and 26 UC patients by hybridization to microarrays interrogating more than 22,000 sequences. Supervised analysis identified a set of 12 genes that distinguished UC and CD patient samples with high accuracy. The alterations in transcript levels observed by microarray were verified by real-time polymerase chain reaction. The results suggest that a peripheral blood mononuclear cell-based gene expression signature can provide a molecular biomarker that can complement the standard diagnosis of UC and CD.
authors.affiliation: Molecular Profiling and Biomarker Discovery/Biomarker Laboratory, Wyeth Research, 500 Arcola Rd., Collegeville PA 19426, USA. mburczynski@wyeth.com
authors.forename: Michael E
authors.initials: ME
authors.lastname: Burczynski
authors.forename: Ron L
authors.initials: RL
authors.lastname: Peterson
authors.forename: Natalie C
authors.initials: NC
authors.lastname: Twine
authors.forename: Krystyna A
authors.initials: KA
authors.lastname: Zuberek
authors.forename: Brendan J
authors.initials: BJ
authors.lastname: Brodeur
authors.forename: Lori
authors.initials: L
authors.lastname: Casciotti
authors.forename: Vasu
authors.initials: V
authors.lastname: Maganti
authors.forename: Padma S
authors.initials: PS
authors.lastname: Reddy
authors.forename: Andrew
authors.initials: A
authors.lastname: Strahs
authors.forename: Fred
authors.initials: F
authors.lastname: Immermann
authors.forename: Walter
authors.initials: W
authors.lastname: Spinelli
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schwertschlag
authors.forename: Anna M
authors.initials: AM
authors.lastname: Slager
authors.forename: Monette M
authors.initials: MM
authors.lastname: Cotreau
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Dorner
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000097:blood / Q000175:diagnosis*; D003424:Crohn Disease / Q000097:blood / Q000175:diagnosis*; D020869:Gene Expression Profiling; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008297:Male; D025202:Molecular Diagnostic Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods