Multiplexed detection of nitrate and nitrite for capillary electrophoresis with an automated device for high injection efficiency.
A simple automated nanoliter scale injection device which allows for reproducible 5 nL sample injections from samples with a volume of <1 microL is successfully used for conventional capillary electrophoresis (CE) and Hadamard transform (HT) CE detection. Two standard fused silica capillaries are assembled axially through the device to function as an injection and a separation capillary. Sample solution is supplied to the injection capillary using pressure controlled with a solenoid valve. Buffer solution flows gravimetrically by the junction of the injection and separation capillaries and is also gated with a solenoid valve. Plugs of sample are pushed into the space between the injection and separation capillaries for electrokinectic injection. To evaluate the performance of the injection device, several optimizations are performed including the influence of flow rates, the injected sample volume and the control of the buffer transverse flow on the overall sensitivity. The system was then applied to HT-CE-UV detection for the signal-to-noise ratio (S/N) improvement of the nitric oxide (NO) metabolites, nitrite and nitrate. In addition, signal averaging was performed to explore the possibility of greater sensitivity enhancements compared to single injections.
Gao
Leyi
L
Department of Chemistry, University of Illinois at Chicago, Illinois 60607-7061, USA.
Patterson
Eric E
EE
Shippy
Scott A
SA
D001331:Automation; D019075:Electrophoresis, Capillary / Q000379:methods*; D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D009566:Nitrates / Q000032:analysis*; D009573:Nitrites / Q000032:analysis; D011076:Polycarboxylate Cement
D009566:Nitrates; D009573:Nitrites; D011076:Polycarboxylate Cement; C005506:polycarbonate