title: Leptin blockade attenuates sodium excretion in saline-loaded normotensive rats.
abstract: Previous investigations in normotensive animals have demonstrated a marked natriuretic and diuretic response following the acute administration of supraphysiologic doses of synthetic leptin. However, the importance of endogenous leptin in the regulation of renal sodium and water balance is not yet defined. This study examined the hemodynamic and renal excretory effects of circulating leptin blockade with a specific polyclonal antibody in groups of normotensive, chronically saline-loaded Sprague-Dawley rats. In the experimental group (n = 10), leptin antibody significantly decreased urinary sodium excretion and urinary flow by approximately 30% compared to the control rats (n = 10). Mean arterial pressure remained unchanged. Collectively, these results are interpreted to suggest that leptin is an important renal sodium-regulating factor under conditions of mild sodium and volume expansion.
authors.affiliation: Department of Internal Medicine, SUNY Upstate Medical University, Syracuse, NY 13210, USA. Villarrd@upstate.edu
authors.forename: Daniel
authors.initials: D
authors.lastname: Villarreal
authors.forename: Garry
authors.initials: G
authors.lastname: Reams
authors.forename: Ronald
authors.initials: R
authors.lastname: Freeman
authors.forename: Robert
authors.initials: R
authors.lastname: Spear
authors.forename: Inna
authors.initials: I
authors.lastname: Tchoukina
authors.forename: Huma
authors.initials: H
authors.lastname: Samar
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D006706:Homeostasis; D006973:Hypertension / Q000139:chemically induced / Q000652:urine; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D020738:Leptin / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000494:pharmacology; D008297:Male; D009318:Natriuresis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012079:Renal Circulation / Q000187:drug effects; D012964:Sodium / Q000652:urine*; D012965:Sodium Chloride / Q000008:administration & dosage*
chemical_list: D020738:Leptin; D012965:Sodium Chloride; D012964:Sodium