Detection and identification of intestinal pathogenic bacteria by hybridization to oligonucleotide microarrays.
OBJECTIVE
To detect the common intestinal pathogenic bacteria quickly and accurately.


METHODS
A rapid (<3 h) experimental procedure was set up based upon the gene chip technology. Target genes were amplified and hybridized by oligonucleotide microarrays.


RESULTS
One hundred and seventy strains of bacteria in pure culture belonging to 11 genera were successfully discriminated under comparatively same conditions, and a series of specific hybridization maps corresponding to each kind of bacteria were obtained. When this method was applied to 26 divided cultures, 25 (96.2%) were identified.


CONCLUSIONS
Salmonella sp., Escherichia coli, Shigella sp., Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, Proteus sp., Bacillus cereus, Vibrio cholerae, Enterococcus faecalis, Yersinia enterocolitica, and Campylobacter jejuni can be detected and identified by our microarrays. The accuracy, range, and discrimination power of this assay can be continually improved by adding further oligonucleotides to the arrays without any significant increase of complexity or cost.
Jin
Lian-Qun
LQ
Tianjin Institute of Hygiene and Environmental Medicine 1, Da Li Road, Tianjin 300050, China. jinlianqun@sina.com
Li
Jun-Wen
JW
Wang
Sheng-Qi
SQ
Chao
Fu-Huan
FH
Wang
Xin-Wei
XW
Yuan
Zheng-Quan
ZQ
D001419:Bacteria / Q000302:isolation & purification*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012336:RNA, Ribosomal, 16S