title: [Nanotechnology-based regenerative medicine--cell sheet engineering utilizing temperature-responsive culture dishes].
abstract: Regenerative medicine is believed to overcome shortcomings of conventional therapies including symptomatic drug therapy and surgical removal. We have utilized nanotechnology for regenerative medicine to develop temperature-responsive culture dishes, on which a temperature-responsive polymer is covalently grafted with precise control of its density and thickness in a nanometer scale. Confluent cells cultured on the dishes are intactly harvested as a single contiguous cell sheet by reducing temperature below 32 degrees C, and harvested cell sheets are subjected to tissue reconstruction, named cell sheet engineering. Here, we introduce the examples of clinical applications including corneal regeneration as well as large animal studies.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000818:Animals; D003315:Cornea / Q000166:cytology; D006801:Humans; D036103:Nanotechnology; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*