title: Evaluation of cytotoxic and mutagenic effects of Coriolus versicolor and Funalia trogii extracts on mammalian cells.
abstract: This study examined the in vitro cytotoxic activities of standardized aqueous bioactive extracts prepared from Coriolus versicolor and Funalia trogiiATCC 200800 on HeLa and fibroblast cell lines using a MTT (3-[4,5-dimetiltiazol-2-]-2-5-difeniltetrazolium bromide) cytotoxicity assay. F. trogii and C. versicolor extracts were cytotoxic to both cell lines. At 10 microL treatment level, F. trogii and C. versicolor extracts inhibited proliferation of HeLa cancer cells by 71.5% and 45%, respectively, compared with controls. Toxicity was lower toward normal fibroblasts. In the latter case, treatment at 10 microL level with F. trogii and C. versicolor extracts reduced cell proliferation by 51.3% and 38.7%, respectively. In separate experiments, the mitotic index (MI) obtained with 3 microL treatment level of unheated extracts of the two fungi was comparable to the MI value obtained by treatment with 4 microg/mL MMC (anticancer agent mitomycin-C). A significant induction of sister chromatid exchange (SCE) was observed in normal cultured lymphocytes treated with MMC (4 microg/mL). MMC treatment reduced replication index compared with treatment with unheated F. trogii extract and negative controls (p < 0.001). In contrast to MMC, F. trogii extracts did not affect the proliferation of human lymphocytes compared with controls (p > 0.05). Laccase and peroxidase enzyme activities in F. trogii extract were implicated in their inhibitory effect on cancer cells. F. trogii extract was concluded to have antitumor activity.
authors.affiliation: Department of Environmental Engineering, Mersin University, Mersin, Turkey. aunyayar@mersin.edu.tr
authors.forename: Ali
authors.initials: A
authors.lastname: Unyayar
authors.forename: Murat
authors.initials: M
authors.lastname: Demirbilek
authors.forename: Melisa
authors.initials: M
authors.lastname: Turkoglu
authors.forename: Ayla
authors.initials: A
authors.lastname: Celik
authors.forename: Mehmet A
authors.initials: MA
authors.lastname: Mazmanci
authors.forename: Emrah A
authors.initials: EA
authors.lastname: Erkurt
authors.forename: Serpil
authors.initials: S
authors.lastname: Unyayar
authors.forename: Ozlem
authors.initials: O
authors.lastname: Cekic
authors.forename: Hatice
authors.initials: H
authors.lastname: Atacag
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D006367:HeLa Cells / Q000187:drug effects / Q000473:pathology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000473:pathology; D008940:Mitotic Index; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity; D010946:Plants, Medicinal / Q000737:chemistry; D012854:Sister Chromatid Exchange / Q000187:drug effects*
chemical_list: D004365:Drugs, Chinese Herbal; D009153:Mutagens; D010936:Plant Extracts