title: Functionalization of gold and glass surfaces with magnetic nanoparticles using biomolecular interactions.
abstract: Advances in nanotechnology have enabled the production and characterization of magnetic particles with nanometer-sized features that can be functionalized with biological recognition elements for numerous applications in biotechnology. In the present study, the synthesis of and interactions between self-assembled monolayers (SAMs) on gold and glass surfaces and functionalized magnetic nanoparticles have been characterized. Immobilization of 10-15 nm streptavidin-functionalized nanoparticles to biotinylated gold and glass surfaces was achieved by the strong interactions between biotin and streptavidin. Fluorescent streptavidin-functionalized nanoparticles, biotinylated surfaces, and combinations of the two were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and electron and fluorescent microscopy to confirm that little or no functionalization occurred in nonbiotinylated regions of the gold and glass surfaces compared to the biotinylated sites. Together these techniques have potential use in studying the modification and behavior of functionalized nanoparticles on surfaces in biosensing and other applications.
authors.affiliation: Department of Biological and Agricultural Engineering, Louisiana State University, Louisiana, USA.
authors.forename: Bala G
authors.initials: BG
authors.lastname: Nidumolu
authors.forename: Michelle C
authors.initials: MC
authors.lastname: Urbina
authors.forename: Josef
authors.initials: J
authors.lastname: Hormes
authors.forename: Challa S S R
authors.initials: CS
authors.lastname: Kumar
authors.forename: W Todd
authors.initials: WT
authors.lastname: Monroe
mesh_terms: D019921:Biotinylation / Q000379:methods*; D005898:Glass / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties
chemical_list: D006046:Gold; D019809:Streptavidin