Acid phosphatase production by Aspergillus niger N402A in continuous flow culture.
The production of acid phosphatases (E.C.3.1.3.2, ACPs) by Aspergillus niger N402A is regulated by specific growth rate, as well as phosphate availability and pH, as demonstrated by studies in continuous flow culture. Specific ACP activity was highest when A. niger was grown at pH 6.3 (64+/-8 U g(-1)) or pH 2.8 (99+/-11 U g(-1)), at a dilution rate of 0.07 h(-1) and phosphate concentrations below 0.46 mM. ACP production was growth correlated for specific growth rates between 0.07 and 0.13 h(-1). Four different ACPs, including two phytases, were produced by A. niger N402A. The ACP and the phytase with maximal activities at pH 5.5 were differentially expressed at different culture pH values, with greater production at low pH.
Hidayat
Budi J
BJ
Department of Life Sciences, Aalborg University, Aalborg, Denmark.
Eriksen
Niels T
NT
Wiebe
Marilyn G
MG
D000135:Acid Phosphatase / Q000096:biosynthesis*; D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development*; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D015966:Gene Expression Regulation, Fungal; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000378:metabolism
D003470:Culture Media; D010710:Phosphates; D000135:Acid Phosphatase