Absorption spectroscopy and binding constants for first-row transition metal complexes of a DOPA-containing peptide.
A diverse array of biological systems incorporate 3,4-dihydroxyphenlyalanine (DOPA) into proteins and small molecules for cross-linking and material generation. Marine worm eggshells, sea squirt wound plugs, and marine mussel adhesives may all be formed by combining DOPA-containing molecules with high levels of metals. In order to provide model systems for characterizing these biomaterials, we carried out a study on metal binding to a DOPA-containing peptide. Ultraviolet-visible absorption spectra are presented for the AdopaTP peptide binding to Fe3+, V3+, VO2+, Mn3+, Ti4+, Cu2+, Co2+, and Ni2+ in mono, bis, and where applicable, tris coordination modes. Association constants were determined for selected metal ions binding to the peptide. In general, the spectroscopic and binding properties of this DOPA-containing peptide were found to be similar to those of catechol.
Sever
Mary J
MJ
Department of Chemistry, Purdue University, West Lafayette, IN, USA.
Wilker
Jonathan J
JJ
D000818:Animals; D049872:Bivalvia / Q000378:metabolism; D003035:Cobalt / Q000737:chemistry; D004295:Dihydroxyphenylalanine / Q000737:chemistry*; D004527:Egg Proteins / Q000737:chemistry; D008345:Manganese / Q000737:chemistry; D008565:Membrane Proteins / Q000378:metabolism; D008670:Metals / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D014025:Titanium / Q000737:chemistry; D028561:Transition Elements / Q000737:chemistry*; D014639:Vanadium / Q000737:chemistry
D004527:Egg Proteins; D008565:Membrane Proteins; D008670:Metals; D010455:Peptides; D028561:Transition Elements; D014639:Vanadium; D003035:Cobalt; D008345:Manganese; D004295:Dihydroxyphenylalanine; D014025:Titanium