title: High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.
abstract: Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
authors.affiliation: Department of Periodontology, University Hospital Münster, Waldeyerstrasse 30, D-48149 Münster, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Aires-de-Sousa
authors.forename: K
authors.initials: K
authors.lastname: Boye
authors.forename: H
authors.initials: H
authors.lastname: de Lencastre
authors.forename: A
authors.initials: A
authors.lastname: Deplano
authors.forename: M C
authors.initials: MC
authors.lastname: Enright
authors.forename: J
authors.initials: J
authors.lastname: Etienne
authors.forename: A
authors.initials: A
authors.lastname: Friedrich
authors.forename: D
authors.initials: D
authors.lastname: Harmsen
authors.forename: A
authors.initials: A
authors.lastname: Holmes
authors.forename: X W
authors.initials: XW
authors.lastname: Huijsdens
authors.forename: A M
authors.initials: AM
authors.lastname: Kearns
authors.forename: A
authors.initials: A
authors.lastname: Mellmann
authors.forename: H
authors.initials: H
authors.lastname: Meugnier
authors.forename: J K
authors.initials: JK
authors.lastname: Rasheed
authors.forename: E
authors.initials: E
authors.lastname: Spalburg
authors.forename: B
authors.initials: B
authors.lastname: Strommenger
authors.forename: M J
authors.initials: MJ
authors.lastname: Struelens
authors.forename: F C
authors.initials: FC
authors.lastname: Tenover
authors.forename: J
authors.initials: J
authors.lastname: Thomas
authors.forename: U
authors.initials: U
authors.lastname: Vogel
authors.forename: H
authors.initials: H
authors.lastname: Westh
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: W
authors.initials: W
authors.lastname: Witte
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods / Q000706:statistics & numerical data; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007753:Laboratories; D015588:Observer Variation; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods / Q000706:statistics & numerical data; D012984:Software; D013203:Staphylococcal Infections / Q000382:microbiology; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D013205:Staphylococcal Protein A