In-vitro and in-vivo antitumour activity of physalins B and D from Physalis angulata.
We have evaluated the in-vitro and in-vivo antitumour activity of physalin B and physalin D isolated from the aerial parts of Physalis angulata. In-vitro, both compounds displayed considerable cytotoxicity against several cancer cell lines, showing IC50 values in the range of 0.58 to 15.18 microg mL(-1) for physalin B, and 0.28 to 2.43 microg mL(-1) for physalin D. The antitumour activity of both compounds was confirmed in-vivo using mice bearing sarcoma 180 tumour cells. The in-vivo antitumour activity was related to the inhibition of tumour proliferation, as observed by the reduction of Ki67 staining in tumours of treated animals. Histopathological examination of the kidney and liver showed that both organs were affected by physalin treatment, but in a reversible manner. These compounds were probably responsible for the previously described antitumour activity of ethanol extracts of P. angulata, and their identification and characterization presented here could explain the ethnopharmacological use of this species in the treatment of cancer.
Magalhães
Hemerson Iury Ferreira
HI
Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CP 3157, Ceará 60430-270, Brazil.
Veras
Maria Leopoldina
ML
Torres
Márcia Rocha
MR
Alves
Ana Paula Negreiros Nunes
AP
Pessoa
Otília Deusdênia Loiola
OD
Silveira
Edilberto Rocha
ER
Costa-Lotufo
Letícia Veras
LV
de Moraes
Manoel Odorico
MO
Pessoa
Cláudia
C
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D032321:Physalis / Q000737:chemistry; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000473:pathology; D012632:Secosteroids; D013256:Steroids / Q000494:pharmacology*; D047368:Tumor Burden / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D007783:Lactones; D012632:Secosteroids; D013256:Steroids; C441429:physalin D; C033270:physalin B