Beta1,4-galactosyltransferase V functions as a positive growth regulator in glioma.
beta1,4-galactosyltransferase V (GalT V; EC 2.4.1.38) can effectively galactosylate the GlcNAcbeta1-->6Man arm of the highly branched N-glycans that are characteristic of glioma. Previously, we have reported that the expression of GalT V is increased in the process of glioma. However, currently little is known about the role of GalT V in this process. In this study, the ectopic expression of GalT V could promote the invasion and survival of glioma cells and transformed astrocytes. Furthermore, decreasing the expression of GalT V in glioma cells promoted apoptosis, inhibited the invasion and migration and the ability of tumor formation in vivo, and reduced the activation of AKT. In addition, the activity of GalT V promoter could be induced by epidermal growth factor, dominant active Ras, ERK1, JNK1, and constitutively active AKT. Taken together, our results suggest that GalT V functioned as a novel glioma growth activator and might represent a novel target in glioma therapy.
Jiang
Jianhai
J
Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Gene Research Center, Shanghai Medical College of Fudan University (former Shanghai Medical University), Shanghai 200032, China.
Chen
Xiaoning
X
Shen
Jialin
J
Wei
Yuanyan
Y
Wu
Tao
T
Yang
Yanzhong
Y
Wang
Hanzhou
H
Zong
Hongliang
H
Yang
Junwu
J
Zhang
Si
S
Xie
Jianhui
J
Kong
Xiangfei
X
Liu
Weicheng
W
Gu
Jianxin
J
D017209:Apoptosis; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D004789:Enzyme Activation; D005700:Galactosyltransferases / Q000096:biosynthesis* / Q000378:metabolism*; D020869:Gene Expression Profiling; D005910:Glioma / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D009361:Neoplasm Invasiveness; D011401:Promoter Regions, Genetic; D014407:Tumor Cells, Cultured
D005700:Galactosyltransferases; C419408:beta-1,4-galactosyltransferase V