title: Fluorinase mediated C-(18)F bond formation, an enzymatic tool for PET labelling.
abstract: The fluorinase enzyme from S. cattleya is applied as a catalyst for the efficient incorporation of [18F]-fluoride into [18F]-5'-fluoro-5'-deoxyadenosine, [18F]-5'-fluoro-5'-deoxyinosine and [18F]-5-fluoro-5-deoxyribose for positron emission tomography (PET) applications.
authors.affiliation: University of St Andrews, School of Chemistry and Centre for Biomolecular Sciences, North Haugh, St Andrews, Fife, UK KY16 9ST.
authors.forename: Hai
authors.initials: H
authors.lastname: Deng
authors.forename: Steven L
authors.initials: SL
authors.lastname: Cobb
authors.forename: Antony D
authors.initials: AD
authors.lastname: Gee
authors.forename: Andrew
authors.initials: A
authors.lastname: Lockhart
authors.forename: Laurent
authors.initials: L
authors.lastname: Martarello
authors.forename: Ryan P
authors.initials: RP
authors.lastname: McGlinchey
authors.forename: David
authors.initials: D
authors.lastname: O'Hagan
authors.forename: Mayca
authors.initials: M
authors.lastname: Onega
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D002384:Catalysis; D003839:Deoxyadenosines / Q000737:chemistry; D005462:Fluorine Radioisotopes / Q000737:chemistry*; D007288:Inosine / Q000031:analogs & derivatives / Q000737:chemistry; D008956:Models, Chemical; D010088:Oxidoreductases / Q000737:chemistry*; D049268:Positron-Emission Tomography / Q000379:methods*; D012267:Ribosemonophosphates / Q000737:chemistry; D013194:Staining and Labeling; D013302:Streptomyces / Q000201:enzymology*; D013997:Time Factors
chemical_list: C514925:5'-fluoro-5'-deoxyadenosine; C507806:5'-fluoro-5-deoxyinosine; C493684:5-fluoro-5-deoxy-D-ribose-1-phosphate; D001426:Bacterial Proteins; D003839:Deoxyadenosines; D005462:Fluorine Radioisotopes; D012267:Ribosemonophosphates; D007288:Inosine; D002244:Carbon; D010088:Oxidoreductases; C495766:fluorinase