Regulation of calcium homeostasis by S100RVP, an androgen-regulated S100 protein in the rat ventral prostate.
BACKGROUND
S100RVP was previously identified as an androgen-response gene in the rat ventral prostate (RVP). Characterization of S100RVP is important for elucidating the function of S100 proteins in androgen action.


METHODS
The expression and subcellular localization of S100RVP were determined by Northern blot, in situ hybridization, and fluorescent microscopy. Calcium overlay and calcium ionophore sensitivity assays were performed to investigate the calcium binding and function of S100RVP.


RESULTS
S100RVP is abundantly expressed in the RVP epithelial cells. A green fluorescent protein(GFP)-S100RVP fusion protein is present in both the cytoplasm and nucleus of transfected cells. A GST-S100RVP fusion protein bound calcium in vitro at levels similar to known S100 proteins. Furthermore, GFP-S100RVP transfected LNCaP and PC3 cells exhibited reduced sensitivity to calcium ionophore-induced cell death, but not to UV-induced cell death.


CONCLUSIONS
The results of this study argue for a role of S100RVP in calcium homeostasis in the prostate.
Oram
Shane
S
University of California at San Francisco, San Francisco VA Medical Center, San Francisco, California, USA.
Cai
Xiaoyan
X
Haleem
Riffat
R
Cyriac
Jomol
J
Wang
Zhou
Z
D000818:Animals; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism*; D006706:Homeostasis; D017403:In Situ Hybridization; D008297:Male; D011467:Prostate / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000502:physiology*
D009418:S100 Proteins; C509970:S100vp protein, rat; D002118:Calcium