Anti-asthmatic activity of an ethanol extract from Saururus chinensis.
As an attempt to find bioactive medicinal herbs exerting anti-asthmatic activity, the effects of an ethanol extract from the parts of Saururus chinensis were evaluated in both in vitro and in vivo. The ethanol extract of S. chinensis (ESC) inhibited generation of the cyclooxygenase-2 (COX-2) dependent phases of prostaglandin D(2) in bone marrow-derived mast cells in a concentration-dependent manner with an IC(50) value of 14.3 microg/ml. ESC also inhibited leukotriene C(4) production with an IC(50) value of 0.3 microg/ml. This demonstrates that ESC has COX-2/5-lipoxygenase dual inhibitory activity. In addition, this compound inhibited degranulation reaction in a dose dependent manner, with an IC(50) value of 1.3 microg/ml. An ovalbumin induced mouse asthmatic animal model was used to determine its in vivo anti-asthmatic activity. The oral administration (50-200 mg/kg) of ESC reduced the number of infiltrated eosinophil in a bronchoalveolar lavage fluid. Furthermore, ESC (100 mg/kg) inhibited the eotaxin and IL-4 mRNA expression levels. These results suggest that the anti-asthmatic activity of S. chinensis might in part occur via the inhibition of eicosanoid generation, degranulation as well as the down regulation of IL-4 and eotaxin mRNA expression.
Lee
Eunkyung
E
College of Pharmacy, Yeungnam University, Gyeongsan 712-749, Korea.
Haa
Kyungmi
K
Yook
Ju Min
JM
Jin
Mei Hua
MH
Seo
Chang Seob
CS
Son
Kun Ho
KH
Kim
Hyun Pyo
HP
Bae
Ki Hwan
KH
Kang
Sam Sik
SS
Son
Jong Keun
JK
Chang
Hyeun Wook
HW
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D002452:Cell Count; D015550:Cell Degranulation / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000166:cytology / Q000276:immunology; D000431:Ethanol / Q000737:chemistry; D005260:Female; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D010047:Ovalbumin / Q000276:immunology; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D015230:Prostaglandin D2 / Q000378:metabolism; D032134:Saururaceae / Q000737:chemistry*
D018927:Anti-Asthmatic Agents; D010936:Plant Extracts; D000431:Ethanol; D010047:Ovalbumin; D001094:Arachidonate 5-Lipoxygenase; D051546:Cyclooxygenase 2; D015230:Prostaglandin D2