Use of molecular beacon probes for real-time PCR detection of Plasmodium falciparum and other plasmodium species in peripheral blood specimens.
We describe the development and evaluation of a novel pair of real-time, fluorescence-based PCR assays using molecular beacon probes for rapid, sensitive, and specific detection and quantification of Plasmodium falciparum and other Plasmodium species organisms.
Elsayed
Sameer
S
Calgary Laboratory Services, 9-3535 Research Rd. NW, Calgary, Alberta, Canada. sameer.elsayed@cls.ab.ca
Plewes
Katherine
K
Church
Deirdre
D
Chow
Barbara
B
Zhang
Kunyan
K
D000818:Animals; D017931:DNA Primers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology; D016778:Malaria, Falciparum / Q000175:diagnosis / Q000469:parasitology; D015336:Molecular Probe Techniques; D018512:Parasitemia / Q000175:diagnosis* / Q000469:parasitology; D010961:Plasmodium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010963:Plasmodium falciparum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
D017931:DNA Primers; D005452:Fluoresceins; D005456:Fluorescent Dyes