title: Structural changes of cell wall mannans of Candida guilliermondii IFO 10279 strain cells cultured at high temperature.
abstract: The morphology, structure, and antigenicity of the cells and the cell wall mannans of the Candida guilliermondii IFO 10279 strain cultivated at 33 and 34 degrees C for 48 h in yeast extract-added Sabouraud liquid medium (YSLM) were compared with those cultivated at 27 degrees C and 33 degrees C and then at 27 degrees C (33-27 degrees C). This strain showed little growth at higher than 35 degrees C. The density of the yeast formed cells decreased, with dry weights of about 50% at 33 and 34 degrees C, and only the cells at 34 degrees C revealed a failure of cytokinesis. The structure of the mannans revealed by (1)H-NMR analysis that the mannans obtained at both 33 and 34 degrees C had drastically decreased two consecutive beta-1,2-linked mannopyranose units at the nonreducing terminal of the alpha-linked oligosaccharides and increased one beta-1,2-linked mannopyranose unit at the nonreducing terminal attached to the alpha-1,3-linked mannose unit and the non-reducing terminal alpha-1,3- and alpha-1,2-linked mannopyranose units. The enzyme-linked immunosorbent assay (ELISA) showed that the mannans obtained at 33 and 34 degrees C had decreased reactivity against the factor serum 9 and increased its reactivity against the factor serum 4, in the commercially available factor serum kit "Candida Check".
authors.affiliation: Second Department of Hygienic Chemistry, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan. okawa@tohoku-pharm.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Okawa
authors.forename: Shingi
authors.initials: S
authors.lastname: Oikawa
authors.forename: Saeko
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D002175:Candida / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D002473:Cell Wall / Q000737:chemistry*; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D008351:Mannans / Q000032:analysis*; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D003470:Culture Media; D008351:Mannans