Influence of crosslinker identity and position on gas-phase dissociation of Lys-Lys crosslinked peptides.
A systematic study of the dissociation patterns of crosslinked peptides analyzed by tandem mass spectrometry is reported. A series of 11-mer peptides was designed around either a polyalanine or polyglycine scaffold with arginine at the C terminus. One or two lysine residues were included at various locations within the peptides to effect inter- or intra-molecular crosslinking, respectively. Crosslinked species were generated with four commonly used amine-specific chemical crosslinking reagents: disuccinimidyl suberate (DSS), disuccinimidyl tartarate (DST), dithiobis(succinimidylpropionate) (DSP), and disuccinimidyl glutarate (DSG). The influence of precursor charge state, location of crosslink, and specific crosslinking reagent on the MS/MS dissociation pattern was examined. Observed trends in the dissociation patterns obtained for these species will allow for improvements to software used in the automated interpretation of crosslinked peptide MS/MS data.
Gaucher
Sara P
SP
Sandia National Laboratories, Livermore, California 94551-0969, USA. spgauch@sandia.gov
Hadi
Masood Z
MZ
Young
Malin M
MM
D001665:Binding Sites; D003432:Cross-Linking Reagents / Q000032:analysis* / Q000737:chemistry*; D005740:Gases / Q000032:analysis / Q000737:chemistry; D008239:Lysine / Q000032:analysis* / Q000737:chemistry*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry*; D044367:Phase Transition; D011485:Protein Binding; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D003432:Cross-Linking Reagents; D005740:Gases; D010455:Peptides; D008239:Lysine