Estimation of aroma glycosides of nutmeg and their changes during radiation processing.
Glycosidically bound volatile compounds of nutmeg were identified as glyco-conjugates of p-cymene-7-ol, eugenol, methoxyeugenol and alpha-terpineol. Using phenyl-beta-glucoside as external standard the contents of these glycosidic precursors were estimated based on the measurement of TLC spot density on a densitometer. p-Cymene-7-ol rutinoside was the major aroma glycoside (3.15 mg/100 g), followed by glucosides of methoxyeugenol (0.61 mg/100 g), eugenol (0.50 mg/100 g) and alpha-terpineol (0.51 mg/100 g). A dose-dependent breakdown of these glycosidic precursors was observed during gamma-radiation processing. Among the four glycosides, alpha-terpineol glucoside was the most sensitive to radiation while p-cymene-7-ol rutinoside was the least sensitive. A reduction in the content of total glycosides by almost 50% was noted at a dose of 5kGy. Partitioning of aroma glycoside into n-butanol from aqueous extracts was found to result in rapid isolation of aroma glycosides, avoiding time consuming pre-purification on Amberlite XAD-2 column. A routine method based on extraction into n-butanol and subsequent quantification of post-irradiation changes in aroma glycosides on a TLC plate using a densitometer is proposed.
Ananthakumar
Arul
A
Food Technology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
Variyar
Prasad S
PS
Sharma
Arun
A
D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006027:Glycosides / Q000032:analysis* / Q000302:isolation & purification; D026323:Myristica / Q000737:chemistry*; D009812:Odorants / Q000032:analysis*; D011839:Radiation, Ionizing
D006027:Glycosides