RNAi dependent epigenetic marks on a geminivirus promoter.
Nicotiana benthamiana plants were stably transformed with an intron-spliced dsRNA producing construct cognate to bidirectional promoter of African cassava mosaic geminivirus (ACMV) DNA A. Transgenic lines expressed multiple siRNAs species upon ACMV infection. The de novo DNA methylation and an increased proportion of histone H3 Lysine-9 methylation (H3K9) at intergenic region (IGR) of ACMV DNA A were observed.
Dogar
Afzal Muhammad
AM
Swiss Institute for Experimental Cancer Research (ISREC), Ch. des Boveresses 155, CH-1066 Epalinges, Switzerland. muhammad.afzaldogar@unil.ch
D019175:DNA Methylation; D044127:Epigenesis, Genetic; D017791:Geminiviridae / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D014026:Nicotiana / Q000821:virology*