Saccharomyces cerevisiae polymerase zeta functions in mitochondria.
The MtArg8 reversion assay, which measures point mutation in mtDNA, indicates that in budding yeast Saccharomyces cerevisiae, DNA polymerase zeta and Rev1 proteins participate in the mitochondrial DNA mutagenesis. Supporting this evidence, both polymerase zeta and Rev1p were found to be localized in the mitochondria. This is the first report demonstrating that the DNA polymerase zeta and Rev1 proteins function in the mitochondria.
Zhang
Hengshan
H
Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
Chatterjee
Aditi
A
Singh
Keshav K
KK
D004272:DNA, Mitochondrial / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004843:Epistasis, Genetic; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D009154:Mutation; D009713:Nucleotidyltransferases / Q000235:genetics*; D017354:Point Mutation; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D000099270:Y-Family DNA Polymerases
D004272:DNA, Mitochondrial; D004259:DNA-Directed DNA Polymerase; D049452:Green Fluorescent Proteins; D009713:Nucleotidyltransferases; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C092219:REV7 protein, S cerevisiae; C100951:REV1 protein, S cerevisiae; D000099270:Y-Family DNA Polymerases; C092602:REV3 protein, S cerevisiae