Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture.
The research involving tissue factors, such as granulocyte macrophage colonies stimulating factor (GM-CSF) and interleukin 5 (IL-5), leads to the mechanisms involved in the maintenance of eosinophilia, which is essential for the pathogenesis on eosinophilic nasal polyps. Mitomycin C has been successfully used in otolaryngology.


OBJECTIVE
The objective of this study was to evaluate the effect of mitomycin C in secretion of GM-CSF and IL-5 on eosinophilic nasal polyps.


METHODS
Case-control.


METHODS
This is a comparative and auto-matched experimental study, performed with fragments of polyps which had been obtained from biopsy of patients with eosinophilic nasosinusal polyposis. The fragments of the experimental group were treated with mitomycin C (400 microg/ml) for 5 minutes and then washed in RPMI substrate. At time zero, 12 and 24 hours, the surface material was taken to determination of its GM-CSF levels in 22 patients and of IL-5 levels in 19 patients, by ELISA method.


RESULTS
Reduction in GM-CSF expression on the experimental group at time 24 h (p< or = 0.05). The treated group presented significant GM-CSF expression between zero time and 12 h time (p= 0.013) showing the culture viability such as in the non-treated group. Tendency to decreasing IL-5 levels on the treated groups at 24 hours.


CONCLUSIONS
This study showed that mitomycin C was efficient in inhibiting GM-CSF synthesis with reduction of IL-5 secretion, but this fact needs complementary studies.
Crosara
Paulo Fernando Tormin Borges
PF
Department of Ophthalmology, Otorhinolaryngology and Speech and Hearing Therapy, Federal University of Minas Gerais. paulocrosara@hc.ufmg.br
Vasconcelos
Anilton César
AC
Guimarães
Roberto Eustáquio Santos
RE
Becker
Helena Maria Gonçalves
HM
Becker
Celso Gonçalves
CG
Crosara
Sandra Letícia Reis
SL
Nascimento
Evaldo
E
D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D016022:Case-Control Studies; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D015848:Interleukin-5 / Q000096:biosynthesis*; D008875:Middle Aged; D016685:Mitomycin / Q000494:pharmacology*; D009298:Nasal Polyps / Q000378:metabolism* / Q000473:pathology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D013997:Time Factors
D015848:Interleukin-5; D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor