title: [Identification of 5 constituents of the aqueous extract of Isatis indigotica by HPLC-MS2].
abstract: OBJECTIVE
  To identify five constituents in the aqueous extract of Isatis indigotica Fort.
  
  
  METHODS
  After separation of the aqueous extract of Isatis indigotica Fort. by HPLC, the eluates of five peaks were collected separatively and analysed by MS2. UV spectra and MS2 were compared with those of reference standards of cytidine, uridine, guanosine xanthine and hypoxanthine.
  
  
  RESULTS
  Each HPLC elute of the aqueous extract had same retention time, UV spectra and fragment pattern in the MS2 spectrum as the corresponding standards.
  
  
  CONCLUSIONS
  Five constituents of the aqueous extract of Isatis indigotica Fort. are identified as cytidine, hypoxanthine, uridine, xanthine and guanosine.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Yuan
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Li
authors.forename: Zhili
authors.initials: Z
authors.lastname: Xiong
authors.forename: Famei
authors.initials: F
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003562:Cytidine / Q000032:analysis / Q000302:isolation & purification*; D006151:Guanosine / Q000032:analysis / Q000302:isolation & purification; D006358:Hot Temperature; D019271:Hypoxanthine / Q000032:analysis / Q000302:isolation & purification; D031219:Isatis / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D014529:Uridine / Q000032:analysis / Q000302:isolation & purification*; D014867:Water; D019820:Xanthine / Q000032:analysis / Q000302:isolation & purification
chemical_list: D014867:Water; D006151:Guanosine; D019820:Xanthine; D019271:Hypoxanthine; D003562:Cytidine; D014529:Uridine