title: Platelet ADP response deteriorates in synthetic storage media.
abstract: BACKGROUND
  During storage under blood bank conditions, platelets (PLTs) are known to secrete ADP. PLT stimulation by ADP results in refractoriness to restimulation, making this response one of the most unstable PLT reactions. The goal of this study was to evaluate the ADP-induced responses of PLTs stored in full plasma or in plasma and additive solution (AS).
  
  
  METHODS
  Surface expression of P-selectin, ADP-induced aggregation, and reconstituted whole-blood thrombus formation were determined on collagen surfaces in a perfusion model with PLTs that were stored for 4 days either in plasma or in the presence of plasma with PAS-II or Composol.
  
  
  RESULTS
  After 4 days of storage in PAS-II but not in Composol, the percentage of PLTs that had secreted granule contents (P-selectin) was increased, when compared to PLTs stored in full plasma. Maximal aggregation in response to ADP was reduced for PLTs stored in PAS-II or Composol. Resuspension of these PLTs in plasma at 37 degrees C for 1 hour caused partial recovery of the aggregation response. Addition of apyrase to PLTs in AS preserved the responsiveness toward ADP. Titration experiments indicated that this response gradually decreased with decreasing plasma concentration. The functional significance of these findings was demonstrated by perfusion experiments. Thrombus formation on collagen was significantly higher for PLTs stored in full plasma than for PLTs stored in PAS-II or Composol.
  
  
  CONCLUSIONS
  Storage of PLTs in the presence of AS under blood bank conditions induces deterioration of the PLT responsiveness to ADP compared to PLT concentrates in 100 percent plasma. Higher plasma-to-AS ratios result in better preserved responses.
authors.affiliation: Sanquin Blood Bank Southeast Region, Maastricht, the Netherlands.
authors.forename: Jeffrey F W
authors.initials: JF
authors.lastname: Keuren
authors.forename: Sandra
authors.initials: S
authors.lastname: Cauwenberghs
authors.forename: Judith
authors.initials: J
authors.lastname: Heeremans
authors.forename: Wim
authors.initials: W
authors.lastname: de Kort
authors.forename: Johan W M
authors.initials: JW
authors.lastname: Heemskerk
authors.forename: Joyce
authors.initials: J
authors.lastname: Curvers
mesh_terms: D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / Q000494:pharmacology; D000244:Adenosine Diphosphate / Q000378:metabolism*; D016718:Arachidonic Acid / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D003094:Collagen / Q000494:pharmacology; D015925:Cryopreservation; D006801:Humans; D019188:Organ Preservation Solutions; D019007:P-Selectin / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology; D010949:Plasma; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D013314:Stress, Mechanical; D014662:Vasoconstrictor Agents / Q000494:pharmacology
chemical_list: D019188:Organ Preservation Solutions; D019007:P-Selectin; D010446:Peptide Fragments; D014662:Vasoconstrictor Agents; C086309:thrombin receptor peptide SFLLRNP; D016718:Arachidonic Acid; D000244:Adenosine Diphosphate; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D003094:Collagen