title: Xanthine oxidase inhibitors from the flowers of Chrysanthemum sinense.
abstract: From the MeOH extract of the flowers of Chrysanthemum sinense, a new flavone glucoside, acacetin 7- O-(3- O-acetyl- beta- D-glucopyranoside), has been isolated together with 27 known compounds including flavonoids, caffeoylquinic acid derivatives, phenolics, and a monoterpenoid glucoside. Their structures were elucidated on the basis of spectroscopic data. Compounds and displayed significant xanthine oxidase inhibitory activity in a concentration-dependent manner, and compounds and showed more potent inhibitory activity, with IC50 values ranging from 0.13 to 2.31 microM, than that of a positive control allopurinol (IC50=2.50 microM). The kinetic study indicated that and displayed competitive-type inhibition like that of allopurinol, while displayed a mixed-type inhibition.
authors.affiliation: Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
authors.forename: Mai Thanh
authors.initials: MT
authors.lastname: Nguyen
authors.forename: Suresh
authors.initials: S
authors.lastname: Awale
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tezuka
authors.forename: Jun-ya
authors.initials: JY
authors.lastname: Ueda
authors.forename: Quan le
authors.initials: Ql
authors.lastname: Tran
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Kadota
mesh_terms: D031202:Chrysanthemum / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
chemical_list: D005419:Flavonoids; D005960:Glucosides; D006027:Glycosides; D039821:Monoterpenes; D010636:Phenols; C477955:acacetin-7-O-glucoside; C472707:caffeoylquinic acid; D011801:Quinic Acid; D014969:Xanthine Oxidase