Calcyclin binding protein promotes DNA synthesis and differentiation in rat neonatal cardiomyocytes.
During cardiac muscle development, most cardiomyocytes permanently withdraw from the cell cycle. Previously, by suppressive subtractive hybridization, we identified calcyclin-binding protein/Siah-interacting protein (CacyBP/SIP) as one of the candidates being upregulated in the hyperplastic to hypertrophic switch, suggesting an important role of CacyBP/SIP in cardiac development. To show the importance of CacyBP/SIP during myoblast differentiation, we report here that CacyBP/SIP is developmentally regulated in postnatal rat hearts. The overexpression of CacyBP/SIP promotes the differentiation and DNA synthesis of H9C2 cells and primary rat cardiomyocytes, as well as downregulates the expression of beta-catenin. Besides, CacyBP/SIP promotes the formation of myotubes and multinucleation upon differentiation. To investigate the cardioprotective role of CacyBP/SIP in cardiomyocytes, a hypoxia/reoxygenation model was employed. We found that CacyBP/SIP was upregulated during myocardial infarction (MI) and hypoxia/reoxygenation. As a conclusion, CacyBP/SIP may play a role in cardiomyogenic differentiation and possibly protection of cardiomyocytes during hypoxia/reoxygenation injury.
Au
Ka-Wing
KW
Department of Biochemistry and Croucher Laboratory for Human Genomics, The Chinese University of Hong Kong, Shatin, NT, China.
Kou
Cecy Y C
CY
Woo
Anthony Y H
AY
Chim
Stephen S C
SS
Fung
Kwok-Pui
KP
Cheng
Christopher H K
CH
Waye
Mary M Y
MM
Tsui
Stephen K W
SK
D000818:Animals; D000831:Animals, Newborn; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002316:Cardiotonic Agents; D002453:Cell Cycle; D002454:Cell Differentiation; D015687:Cell Hypoxia; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D015536:Down-Regulation / Q000235:genetics; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D047908:Intracellular Signaling Peptides and Proteins; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000235:genetics; D051176:beta Catenin / Q000235:genetics
C491777:CacyBP protein, rat; D002135:Calcium-Binding Proteins; D002316:Cardiotonic Agents; D003470:Culture Media; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D051176:beta Catenin; D004247:DNA; D003402:Creatine Kinase