title: Reprogramming of human somatic cells by embryonic stem cell cytoplast.
abstract: Somatic cell nuclear transfer (SCNT) provides the basis for the development of patient-specific stem cell lines. Recent progress in SCNT suggested the presence of reprogramming factors in human embryonic stem (hES) cells, although no method is currently available for replacement of nuclei of hES cells by somatic cell nuclei. An original technique has been developed, involving the fusion of different types of somatic cells with hES cells, which allowed a complete replacement of the nuclei of hES cells by nuclei of somatic cells. The resulting 'cybrids' were demonstrated to have the genotype of the donor somatic cells and 'stemness' of the recipient hES cells. However, the colonies isolated from the resulting fusion contained a mixture of these cybrid cells with the cells with the recipient nuclei, as well as hybrid cells containing both donor and recipient nuclei, so future purification will be necessary before the technique can be considered for future practical application.
authors.affiliation: Reproductive Genetics Institute, 2825 North Halsted Street, Chicago, IL 60657, USA.
authors.forename: Nick
authors.initials: N
authors.lastname: Strelchenko
authors.forename: Valeri
authors.initials: V
authors.lastname: Kukharenko
authors.forename: Artem
authors.initials: A
authors.lastname: Shkumatov
authors.forename: Oleg
authors.initials: O
authors.lastname: Verlinsky
authors.forename: Anver
authors.initials: A
authors.lastname: Kuliev
authors.forename: Yury
authors.initials: Y
authors.lastname: Verlinsky
mesh_terms: D002459:Cell Fusion / Q000379:methods*; D049109:Cell Proliferation; D003593:Cytoplasm / Q000502:physiology*; D004622:Embryo, Mammalian / Q000166:cytology*; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology*; D017404:In Situ Hybridization, Fluorescence; D053652:Nuclear Transfer Techniques; D013234:Stem Cells / Q000166:cytology*
chemical_list: D016650:Fluorescein-5-isothiocyanate