Mathematical modeling of calcium homeostasis in yeast cells.
In this study, based on currently available experimental observations on protein level, we constructed a mathematical model to describe calcium homeostasis in normally growing yeast cells (Saccharomyces cerevisiae). Simulation results show that tightly controlled low cytosolic calcium ion level can be a natural result under the general mechanism of gene expression feedback control. The calmodulin (a sensor protein) behavior in our model cell agrees well with relevant observations in real cells. Moreover, our model can qualitatively reproduce the experimentally observed response curve of real yeast cell responding to step-like disturbance in extracellular calcium ion concentration. Further investigations show that the feedback control mechanism in our model is as robust as it is in real cells.
Cui
Jiangjun
J
Section Computational Science, Faculty of Science, University of Amsterdam, The Netherlands.
Kaandorp
Jaap A
JA
D019703:Calcineurin / Q000378:metabolism; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D015220:Calcium Channels / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000235:genetics / Q000502:physiology; D002147:Calmodulin / Q000378:metabolism; D003198:Computer Simulation; D015966:Gene Expression Regulation, Fungal; D006706:Homeostasis; D008954:Models, Biological; D008962:Models, Theoretical; D050505:Mutant Proteins / Q000502:physiology; D053499:Plasma Membrane Calcium-Transporting ATPases; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology
D015220:Calcium Channels; D002147:Calmodulin; D050505:Mutant Proteins; C085484:PMC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D019703:Calcineurin; D053499:Plasma Membrane Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases; D002118:Calcium