Low intensity pulsed ultrasound accelerated bone remodeling during consolidation stage of distraction osteogenesis.
Bone regeneration in distraction osteogenesis occurs under tensile stress with axial rhythmic distraction after osteotomy. In this study, we evaluated if the low intensity pulsed ultrasound (LIPUS) was also effective on enhancement of bone remodeling during consolidation stage of distraction osteogenesis. Open osteotomy of seventeen 18-week-old female New Zealand rabbit tibiae were performed. The distraction was applied with the rate of 1 mm per day. LIPUS (30 mW/cm(2), 1.5 MHz) was delivered for 20 min per day during 4-week consolidation stage (n=10). The animals without treatment served as sham group (n=7). Plain X-ray, peripheral quantitative computational tomography (pQCT), and torsional test were performed. Results showed that smaller radiolucent interzone of LIPUS treatment group was gradually occupied by calcified tissue in plain X-ray at week 2. The bone mineral density (BMD) measured on radiographs increased by 9.18% in the LIPUS group. Bone mineral content (BMC), hard callus volume, and bone strength index (BSI) measured by pQCT were 83%, 116%, and 94%, respectively, in LIPUS group that were significantly greater than those of the controls. At the 4th week, LIPUS-treated callus showed the development of neocorticalization in the proximal and distal region. The BMC, hard callus volume, and BSI of LIPUS group decreased and was not significantly different from control. This was also confirmed by the maximum torque of LIPUS-treated callus (1424.2+/-457.3 N . mm) obtained at week 4, which did not differ from that of the sham group (1968.8+/-895.1 N . mm). In conclusion, the effective period of LIPUS treatment was at the initial stage of consolidation, with accelerated bone formation and remodeling.
Chan
Chun Wai
CW
Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T., Hong Kong.
Qin
Ling
L
Lee
Kwong Man
KM
Zhang
Ming
M
Cheng
Jack Chun Yiu
JC
Leung
Kwok Sui
KS
D000818:Animals; D015519:Bone Density; D016723:Bone Remodeling / Q000502:physiology*; D002146:Bony Callus / Q000000981:diagnostic imaging* / Q000378:metabolism; D005260:Female; D019857:Osteogenesis, Distraction; D010027:Osteotomy / Q000534:rehabilitation*; D011817:Rabbits; D013314:Stress, Mechanical; D013977:Tibia / Q000000981:diagnostic imaging* / Q000378:metabolism; D014057:Tomography, X-Ray Computed; D014102:Torsion Abnormality; D014464:Ultrasonic Therapy; D014463:Ultrasonography; D016474:Weight-Bearing