title: Streptozotocin (STZ) diabetes enhances benzo(alpha)pyrene induced renal injury in Sprague Dawley rats.
abstract: Information is lacking regarding the biological response to environmental chemicals in the context of pre-existing disease. Benzo(alpha)pyrene (BaP), a polycyclic aromatic hydrocarbon, is a byproduct of combustion that causes renal injury and elicits a nephropathic response. This study evaluated the nephrotoxicity of BaP in normoglycemic and diabetic rats. Female Sprague Dawley rats were divided into four groups: normoglycemic-vehicle (NV), normoglycemic-BaP (N-BaP), diabetic-vehicle (DV) and diabetic-BaP (D-BaP). Diabetes was induced by intraperitoneal (ip) injection of streptozotocin (60 mg/kg, 1 ml/kg). Rats were injected (ip) with vehicle or 10 mg/kg BaP (1 ml/kg) once per week for 5 weeks. Urinary protein and albumin, plasma creatinine and light microscopy were performed to assess the effects of BaP on kidney function. Diabetes was confirmed by plasma glucose levels >400 mg/dl in the DV and D-BaP groups. BaP increased kidney weight and blood urea nitrogen (BUN) levels in the D-BaP relative to the DV group. No change in BUN was observed following 5 weeks of BaP treatment in the normoglycemic animals, however, kidney weight was increased (p=0.013) in the N-BaP relative to the NV animals. STZ diabetes increased susceptibility to BaP mediated renal damage following repeated treatment for 5 weeks when compared to age matched normoglycemic rats.
authors.affiliation: Department of Pharmacology, Joan C. Edwards School Of Medicine, Marshall University1532 Spring Valley Drive, Huntington, WV 25704-9388, USA. valentov@marshall.edu
authors.forename: Monica A
authors.initials: MA
authors.lastname: Valentovic
authors.forename: Napoleon
authors.initials: N
authors.lastname: Alejandro
authors.forename: A
authors.initials: A
authors.lastname: Betts Carpenter
authors.forename: Patrick I
authors.initials: PI
authors.lastname: Brown
authors.forename: Kenneth
authors.initials: K
authors.lastname: Ramos
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D004198:Disease Susceptibility; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000209:etiology* / Q000473:pathology; D007677:Kidney Function Tests; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001786:Blood Glucose; D004785:Environmental Pollutants; D001564:Benzo(a)pyrene