title: sucAB and sucCD are mutually essential genes in Escherichia coli.
abstract: sucAB and sucCD of Escherichia coli encode enzymes that generate succinyl-CoA from 2-oxoglutarate and succinate, respectively. Their mutual essentiality was studied. sucAB and sucCD could be deleted individually, but not simultaneously. The mutual essentiality of sucAB and sucCD was further confirmed by the conditional expression of sucABCD, sucAB, and sucCD under the control of a P(BAD) in E. coli MG1655, E. coli MG1655 (DeltasucCD), and E. coli MG1655 (DeltasucAB), respectively. These strains grew well in Luria-Bertani medium containing 0.1% arabinose, but not in the absence of arabinose unless the medium was supplemented with succinyl-CoA. Our results indicate that either sucAB or sucCD is enough to produce succinyl-CoA that is essential for cell viability.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea.
authors.forename: Byung Jo
authors.initials: BJ
authors.lastname: Yu
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Sung
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Sung Hwa
authors.initials: SH
authors.lastname: Son
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Sun Chang
authors.initials: SC
authors.lastname: Kim
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D020043:Genes, Essential; D007655:Ketoglutarate Dehydrogenase Complex / Q000235:genetics / Q000378:metabolism*; D013389:Succinate-CoA Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000214:Acyl Coenzyme A; D029968:Escherichia coli Proteins; C012046:succinyl-coenzyme A; D007655:Ketoglutarate Dehydrogenase Complex; D013389:Succinate-CoA Ligases