Dominulin A and B: two new antibacterial peptides identified on the cuticle and in the venom of the social paper wasp Polistes dominulus using MALDI-TOF, MALDI-TOF/TOF, and ESI-ion trap.
Two new antibacterial peptides, denominated as Dominulin A and B, have been found on the cuticle and in the venom of females of the social paper wasp Polistes dominulus. The amino acidic sequence of the two peptides, determined by mass spectrometry, is INWKKIAE VGGKIL SSL for Dominulin A (MW = 1854 Da) and INWKKIAEIGKQVL SAL (MW = 1909 Da) for Dominulin B. Their presence on the cuticle was confirmed using MALDI-TOF by means of micro-extractions and direct analyses on body parts. The presence in the venom and the primary structure of the dominulins suggest their classification in the mastoparans, a class of peptides found in the venom of other Aculeate hymenoptera. Their antimicrobial action against Gram+ and Gram- bacteria fits in the range of the best natural antimicrobial peptides. Dominulins can represent an important defense of the colony of Polistes dominulus against microbial pathogens.
Turillazzi
Stefano
S
Centro Interdipartimentale di Spettrometria di Massa, Università di Firenze, Firenze, Italy. turillazzi@dbag.unifi.it
Mastrobuoni
Guido
G
Dani
Francesca R
FR
Moneti
Gloriano
G
Pieraccini
Giuseppe
G
la Marca
Giancarlo
G
Bartolucci
Gianluca
G
Perito
Brunella
B
Lambardi
Duccio
D
Cavallini
Vanni
V
Dapporto
Leonardo
L
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000032:analysis / Q000494:pharmacology; D001412:Bacillus subtilis / Q000166:cytology / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods; D012867:Skin / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014862:Wasp Venoms / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology; D014863:Wasps / Q000378:metabolism*
D023181:Antimicrobial Cationic Peptides; D014862:Wasp Venoms; C510985:dominulin A; C510986:dominulin B