Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from North Brazil.
We have examined the prevalence of gene cagA and vacA alleles in 129 patients, 69 with gastritis and 60 with peptic ulcer diseases from North Brazil and their relation with histopathological data. vacA and cagA genotype were determined by polymerase chain reaction. Hematoxylin-eosin staining was used for histological diagnosis. 96.6% of the patients were colonized by Helicobacter pylori strains harboring single vacA genotype (nont-mixed infection). Among them, 11.8% had subtype s1a, 67.8% had subtype s1b, and 17% subtype s2. In regard to the middle region analysis, m1 alleles were found in 75.4% and m2 in 21.2% of patients. The cagA gene was detected in 78% patients infected with H. pylori and was associated with the s1-m1 vacA genotype. The H. pylori strains, vacA s1b m1/cagA-positive, were associated with increased risk of peptic ulcer disease and higher amounts of lymphocytic and neutrophilic infiltrates and the presence of intestinal metaplasia. These findings show that cagA and vacA genotyping may have clinical relevance in Brazil.
Martins
Luisa Caricio
LC
Laboratório de Imunogenética, Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil. lu-caricio@bol.com.br
Corvelo
Tereza Cristina de Oliveira
TC
Demachki
Samia
S
Araujo
Marialva T F
MT
Assumpção
Mônica Baraúna
MB
Vilar
Simone Cristina Araujo Jucá
SC
Freitas
Felipe Bonfim
FB
Barbosa
Hivana Patricia Melo
HP
Fecury
Amanda Alves
AA
do     Amaral
Renata Kelly Costa
RK
Dos         Santos
Sidney Emanuel Batista
SE
D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001938:Brazil; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005756:Gastritis / Q000382:microbiology* / Q000473:pathology; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010437:Peptic Ulcer / Q000382:microbiology* / Q000473:pathology; D016133:Polymerase Chain Reaction
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori