Specific distribution of gabarap, gec1/gabarap Like 1, gate16/gabarap Like 2, lc3 messenger RNAs in rat brain areas by quantitative real-time PCR.
GABARAP and GEC1/GABARAPL1 interact with tubulin and GABA(A) receptor and belong to a new protein family. This family includes GATE 16 and LC3, potentially involved in intracellular transport processes. In this study, we combined brain dissection and quantitative real-time reverse transcription polymerase chain reaction to study discriminatively gabarap, gec1/gabarapL1, gate16/gabarapL2, lc3 mRNA distribution in multiple rat brain areas.
Mansuy-Schlick
Virginie
V
Université de Franche-Comté, IFR 133, U.F.R. Sciences et Techniques, Equipe Estrogènes, Expression Génique et Pathologies du Système Nerveux Central, 16 route de Gray, 25030 Besançon cedex, France.
Tolle
Fabrice
F
Delage-Mourroux
Régis
R
Fraichard
Annick
A
Risold
Pierre-Yves
PY
Jouvenot
Michèle
M
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
C502186:GABARAP protein, rat; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger