title: A new diagnostic indicator using concanavalin a low-affinity lactoferrin levels in mammary gland secretion in mastitic drying cows.
abstract: We examined the effective diagnostic indicator using the concanavalin A (Con A) low-affinity lactoferrin (Lf) to mastitic drying cows. The concentrations of both Lf and Con A low-affinity Lf in mammary gland secretions (MGSs) were lower than normal MGSs at the early and middle dry periods and colostrums. On the other hand, the levels of Con A low-affinity Lf in MGSs increased following the appearance of mastitis symptoms, and decreased when the mastitic symptoms were cured. Moreover, IgG1 concentrations of colostrums decrease on the quarters where a high level of Con A low-affinity Lf was determined after the onset of dry period. These results suggest that this method could be used as a useful indicator to mastitic drying cows.
authors.affiliation: T-Cell Research Institute, In Kyouritsu Seiyaku Co., Tokyo 102-0074, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Komine
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Komine
authors.forename: Kenzou
authors.initials: K
authors.lastname: Kai
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Kuroishi
authors.forename: Masashi
authors.initials: M
authors.lastname: Itagaki
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Obara
authors.forename: Katsuo
authors.initials: K
authors.lastname: Kumagai
mesh_terms: D000818:Animals; D002417:Cattle; D003126:Colostrum / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism*; D005260:Female; D007074:Immunoglobulin G / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism; D008414:Mastitis, Bovine / Q000175:diagnosis*
chemical_list: D007074:Immunoglobulin G; D003208:Concanavalin A; D007781:Lactoferrin