[Identification of 5 constituents of the aqueous extract of Isatis indigotica by HPLC-MS2].
OBJECTIVE
To identify five constituents in the aqueous extract of Isatis indigotica Fort.


METHODS
After separation of the aqueous extract of Isatis indigotica Fort. by HPLC, the eluates of five peaks were collected separatively and analysed by MS2. UV spectra and MS2 were compared with those of reference standards of cytidine, uridine, guanosine xanthine and hypoxanthine.


RESULTS
Each HPLC elute of the aqueous extract had same retention time, UV spectra and fragment pattern in the MS2 spectrum as the corresponding standards.


CONCLUSIONS
Five constituents of the aqueous extract of Isatis indigotica Fort. are identified as cytidine, hypoxanthine, uridine, xanthine and guanosine.
Liu
Rui
R
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016.
Yuan
Bo
B
Liu
Zhigang
Z
Li
Xiaoqing
X
Xiong
Zhili
Z
Li
Famei
F
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003562:Cytidine / Q000032:analysis / Q000302:isolation & purification*; D006151:Guanosine / Q000032:analysis / Q000302:isolation & purification; D006358:Hot Temperature; D019271:Hypoxanthine / Q000032:analysis / Q000302:isolation & purification; D031219:Isatis / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D014529:Uridine / Q000032:analysis / Q000302:isolation & purification*; D014867:Water; D019820:Xanthine / Q000032:analysis / Q000302:isolation & purification
D014867:Water; D006151:Guanosine; D019820:Xanthine; D019271:Hypoxanthine; D003562:Cytidine; D014529:Uridine