[Common bile duct ligation as a model of hepatopulmonary syndrome and oxidative stress].
BACKGROUND
The hepatopulmonary syndrome is characterized by hepatic dysfunction and presence of dilated pulmonary vessels, with alterations in air diffusion that can be demonstrated in the experimental model of common bile duct ligation.


OBJECTIVE
To evaluate the oxidative stress in pulmonary tissue of cirrhotic rats with common bile duct ligation.


METHODS
We used 12 male Wistar rats weighing between 200-300 g divided in two groups: control (Co = 6) and cirrhotic (Ci = 6). We evaluated aminotransferases, arterial gasometry, lipoperoxidation and chemoluminescence), and antioxidant enzymatic activity with superoxide dismutase. The tissues analyzed for hepatopulmonary syndrome were cirrhotic liver and lung.


RESULTS
The animals with common bile duct ligation showed alterations in the following aminotransferases: aspartate aminotransferase, Co = 105.3 +/- 43/Ci = 500.5 +/- 90.3, alanine aminotransferase, Co = 78.75 +/- 37.7/Ci = 162.75 +/- 35.4, and alkaline phosphatase, Co = 160 +/- 20.45/Ci = 373 +/- 45.44. The lipoperoxidation and the antioxidant response had significant differences between the groups when evaluated in lung (lipoperoxidation) Co = 0.87 +/- 0.3/Ci = 2.01 +/- 0.9, chemoluminescence Co = 16008.41 +/- 1171.45/Ci = 20250.36 +/- 827.82 superoxide dismutase Co = 6.66 +/- 1.34/Ci = 16.06 +/- 2.67.


CONCLUSIONS
Our results suggest that in this experimental model of cirrhosis using common bile duct ligation, there is an increase in lipoperoxidation in pulmonary tissue as well as an increase in superoxide dismutase's antioxidant activity, suggesting a pulmonary injury caused by secondary biliary cirrhosis.
Tieppo
Juliana
J
Laboratório de Fisiologia Digestiva e Estresse Oxidativo, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS.
Vercelino
Rafael
R
Dias
Alexandre Simões
AS
Marroni
Cláudio Augusto
CA
Marroni
Norma
N
D000818:Animals; D001784:Blood Gas Analysis; D003135:Common Bile Duct / Q000601:surgery*; D004195:Disease Models, Animal; D020065:Hepatopulmonary Syndrome / Q000201:enzymology / Q000209:etiology / Q000378:metabolism*; D008026:Ligation; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000032:analysis*; D017392:Thiobarbituric Acid Reactive Substances; D000637:Transaminases / Q000097:blood*
D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase; D000637:Transaminases