title: Use of different stains for microscopic evaluation of corneal scrapings for diagnosis of microsporidial keratitis.
abstract: Retrospective evaluation of potassium hydroxide plus calcofluor white (KOH+CFW), Gram, Giemsa, and modified Ziehl-Neelsen (1% H(2)SO(4), cold) stains for the detection of microsporidia in corneal scrapings from 30 patients showed KOH+CFW and acid-fast stains to be most efficient (29/30 [96.7%] and 28/30 [93.3%], respectively) in the diagnosis of microsporidial keratitis.
authors.affiliation: Jhaveri Microbiology Centre, L.V. Prasad Eye Institute, L.V. Prasad Marg, Banjara Hills, Hyderabad 500034, India. savitri@lvpei.org
authors.forename: Joveeta
authors.initials: J
authors.lastname: Joseph
authors.forename: Somasheila
authors.initials: S
authors.lastname: Murthy
authors.forename: Prashant
authors.initials: P
authors.lastname: Garg
authors.forename: Savitri
authors.initials: S
authors.lastname: Sharma
mesh_terms: D001557:Benzenesulfonates / Q000378:metabolism; D003315:Cornea / Q000469:parasitology*; D015822:Eye Infections, Parasitic / Q000175:diagnosis / Q000469:parasitology; D006801:Humans; D006878:Hydroxides / Q000378:metabolism; D007634:Keratitis / Q000175:diagnosis* / Q000469:parasitology*; D016814:Microsporidia / Q000302:isolation & purification*; D016881:Microsporidiosis / Q000175:diagnosis / Q000469:parasitology; D017680:Potassium Compounds / Q000378:metabolism; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D001557:Benzenesulfonates; D006878:Hydroxides; D017680:Potassium Compounds; C007061:C.I. Fluorescent Brightening Agent 28; C029943:potassium hydroxide