title: Genetic diversity of Legionella pneumophila inferred from rpoB and dotA sequences.
abstract: This study characterised the population structure of Legionella pneumophila by comparing the rpoB (300-bp) and dotA (360-bp) sequences of 267 isolates (18 reference strains, 149 Korean isolates and 100 Japanese isolates). In addition to the six clonal subgroups established previously, four subgroups, P-V to P-VIII, were identified. Subgroupings based on rpoB and dotA sequences were found to correlate with the source of the isolates, and this data may be useful for future epidemiological studies. Fourteen (five Korean and nine Japanese) isolates showed incongruent subgroupings in the rpoB and dotA trees, suggesting that genetic exchange among subgroups, and even among subspecies, may occur frequently in nature.
authors.affiliation: Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul, Korea.
authors.forename: K S
authors.initials: KS
authors.lastname: Ko
authors.forename: H
authors.initials: H
authors.lastname: Miyamoto
authors.forename: H K
authors.initials: HK
authors.lastname: Lee
authors.forename: M-Y
authors.initials: MY
authors.lastname: Park
authors.forename: K
authors.initials: K
authors.lastname: Fukuda
authors.forename: B J
authors.initials: BJ
authors.lastname: Park
authors.forename: Y-H
authors.initials: YH
authors.lastname: Kook
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D007564:Japan; D007723:Korea; D016952:Legionella pneumophila / Q000145:classification / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C440002:DotA protein, Legionella pneumophila; D008565:Membrane Proteins; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit