title: Intra-adrenal interactions in fish: catecholamine stimulated cortisol release in sea bass (Dicentrarchus labrax L.).
abstract: The effect of the catecholamines, adrenaline and noradrenaline, on sea bass (Dicentrarchus labrax) and sea bream (Sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system technique. Increasing concentrations of catecholamines (10(-6), 10(-8) and 10(-10) M) stimulated cortisol production in a dose-dependent manner, in sea bass only. The increase in cortisol production stimulated by adrenaline (10(-6) M) and noradrenaline (10(-6) M) was inhibited by sotalol (2 x 10(-5) M), but not by prazosin suggesting that catecholamines stimulate cortisol release through the beta-receptor subtype. To evaluate catecholamine-induced signal transduction in head kidney cells, measurements of cAMP production and [H3]myo-inositol incorporation were determined in head kidney cell suspensions. Adrenaline and noradrenaline (10(-6) M) increased cAMP production, but had no effect on total inositol phosphate accumulation. These results indicate that catecholamines released from the chromaffin cells within the interrenal tissue may act as a paracrine factor to stimulate interrenal steroidogenesis in the sea bass.
authors.affiliation: Centre for Marine Sciences (CCMAR), Campus de Gambelas, University of Algarve, 8005-139 Faro, Portugal. rotllant@umbi.umd.edu
authors.forename: Josep
authors.initials: J
authors.lastname: Rotllant
authors.forename: Neil M
authors.initials: NM
authors.lastname: Ruane
authors.forename: Maria T
authors.initials: MT
authors.lastname: Dinis
authors.forename: Adelino V M
authors.initials: AV
authors.lastname: Canario
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Power
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001492:Bass / Q000502:physiology*; D004837:Epinephrine / Q000494:pharmacology*; D006854:Hydrocortisone / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism; D007401:Interrenal Gland / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology*; D011224:Prazosin / Q000494:pharmacology; D015290:Second Messenger Systems / Q000502:physiology; D013015:Sotalol / Q000494:pharmacology
chemical_list: D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D007294:Inositol; D013015:Sotalol; D006854:Hydrocortisone; D009638:Norepinephrine; D011224:Prazosin; D004837:Epinephrine