A comparative study of capacitive immunosensors based on self-assembled monolayers formed from thiourea, thioctic acid, and 3-mercaptopropionic acid.
A procedure was developed for the covalent coupling of anti-alpha-fetoprotein antibody (anti-AFP) to a gold surface modified with a self-assembled monolayer (SAM) of thiourea (TU). The performance of the SAM-antibody layer was compared to those of similar layers based on thioctic acid (TA) and 3-mercaptopropionic acid (MPA) by using flow injection capacitive immunosensor system. Covalent coupling of anti-AFP on self-assembled thiourea monolayer (SATUM) modified gold electrode can be used to detect alpha-fetoprotein with high efficiency, similar sensitivity, the same linear range (0.01-10 microgl(-1)) and detection limit (10 ngl(-1)) as those obtained from sensors based on self-assembled thioctic acid monolayer (SATAM) and self-assembled 3-mercaptopropionic acid monolayer (SAMPAM). The system is specific for alpha-fetoprotein and can be regenerated and reused up to 48 times. Therefore, self-assembled monolayer using thiourea which is cheaper than thioctic acid and 3-mercaptopropionic acid is a good alternative for biosensor applications when SAMs are used.
Limbut
Warakorn
W
Biophysics Research Unit, Biosensors and Biocurrents, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Kanatharana
Proespichaya
P
Mattiasson
Bo
B
Asawatreratanakul
Punnee
P
Thavarungkul
Panote
P
D015097:3-Mercaptopropionic Acid; D000906:Antibodies; D015374:Biosensing Techniques / Q000295:instrumentation*; D008063:Thioctic Acid; D013890:Thiourea; D000509:alpha-Fetoproteins / Q000276:immunology
D000906:Antibodies; D000509:alpha-Fetoproteins; D008063:Thioctic Acid; D015097:3-Mercaptopropionic Acid; D013890:Thiourea