Cumulative effects of short-term polymetal contamination on soil bacterial community structure.
In this study we evaluated the short-term effects of copper, cadmium, and mercury, added singly or in combination at different doses, on soil bacterial community structure using the bacterial automated ribosomal intergenic spacer analysis (B-ARISA) fingerprinting technique. Principal-component analysis of B-ARISA profiles allowed us to deduce the following order of impact: (Cu + Cd + Hg) >> Hg > or = Cd > Cu. These results demonstrated that there was a cumulative effect of metal toxicity. Furthermore, the trend of modifications was consistent with the "hump-backed" relationships between biological diversity and disturbance described by Giller et al. (K. E. Giller, E. Witler, and S. P. McGrath, Soil Biol. Biochem. 30:1389-1414, 1998).
Ranjard
L
L
INRA/Université de Bourgogne, UMR Microbiologie et Géochimie des Sols, CMSE, 17 rue de Sully, B.V. 86510, 21065 Dijon Cedex, France. lionel.ranjard@dijon.inra.fr
Lignier
L
L
Chaussod
R
R
D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D002104:Cadmium / Q000633:toxicity; D003300:Copper / Q000633:toxicity; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000235:genetics / Q000302:isolation & purification; D017753:Ecosystem; D008628:Mercury / Q000633:toxicity; D019216:Metals, Heavy / Q000633:toxicity*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000633:toxicity*
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D019216:Metals, Heavy; D012989:Soil Pollutants; D002104:Cadmium; D003300:Copper; D008628:Mercury