Isolation and characterization of Halomonas sp. strain IMPC, a p-coumaric acid-metabolizing bacterium that decarboxylates other cinnamic acids under hypersaline conditions.
A moderately halophilic, mesophilic, Gram-negative, motile, nonsporulating bacterium, designated strain IMPC, was isolated from a table-olive fermentation rich in aromatic compounds, after enrichment on p-coumaric acid under halophilic conditions. Strain IMPC was able to degrade p-coumaric acid. p-hydroxybenzaldehyde and p-hydroxybenzoic acid were detected as breakdown products from p-coumaric acid. Protocatechuic acid was identified as the final aromatic product of p-coumaric acid catabolism before ring fission. Strain IMPC transformed various cinnamic acids with substituent H, OH, CH(3) or OCH(3) in the para- and/or meta-position of the aromatic ring to the corresponding benzoic acids, indicating a specific selection. A beta-oxidation pathway was proposed for these transformations. Phylogenetic analysis of the 16S rRNA gene revealed that this isolate was a member of the genus Halomonas. Strain IMPC was closely related to Halomonas elongata ATCC 33173(T)and Halomonas eurihalina ATCC 49336(T).
Abdelkafi
Slim
S
Ecologie Microbienne d'Environnements Naturels et Anthoropisés UR 180, IRD, IFR-BAIM, Universités de Provence et de la méditerranée, ESIL case 925, Marseille cedex 9, France.
Labat
Marc
M
Casalot
Laurence
L
Chamkha
Mohamed
M
Sayadi
Sami
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002934:Cinnamates / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D003653:Decarboxylation; D020632:Halomonas / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism*; D011422:Propionates; D012965:Sodium Chloride
D000900:Anti-Bacterial Agents; D002934:Cinnamates; D003373:Coumaric Acids; D004269:DNA, Bacterial; D011422:Propionates; C029010:cinnamic acid; D012965:Sodium Chloride; C495469:p-coumaric acid