title: Changes of physical and biochemical properties of Cryptosporidium oocysts with various storage conditions.
abstract: Physical and biochemical properties of Cryptosporidium parvum oocyst were examined after storage under various conditions. Oocyst-positive-fecal samples recovered from calves were either stored in a 2.0% potassium dichromate solution (Cr) or deionized water (W), or kept as a fecal pellet (P), and stored at 4 or 18 degrees C for a maximum of 100 days. When stored in Cr at 4 degrees C, the morphology of oocysts and their ability to withstand ultrasonics was not affected by the storing media or the storage period. However, when stored at 18 degees C as a fecal pellet, the specific gravity of the oocysts increased and a significant decrease in the oocysts resistance to ultrasonics occurred. These changes in oocyst properties may affect the performance of methods used to detect oocysts in water samples. When using the current test methods or when developing a new test method, it is important to take these factors into consideration.
authors.affiliation: Water Quality Management Center, Enterprises Agency, Hyogo Prefecture Government, 3-1, Nagahara, Tai, Kande-cho, Nishi-ku, Kobe 651-2313, Japan. Motomu_Inoue@pref.hyogo.jp
authors.forename: Motomu
authors.initials: M
authors.lastname: Inoue
authors.forename: Shoji
authors.initials: S
authors.lastname: Uga
authors.forename: Takuya
authors.initials: T
authors.lastname: Oda
authors.forename: Shiba Kumar
authors.initials: SK
authors.lastname: Rai
authors.forename: Graham
authors.initials: G
authors.lastname: Vesey
authors.forename: Hak
authors.initials: H
authors.lastname: Hotta
mesh_terms: D000818:Animals; D002417:Cattle; D016785:Cryptosporidium parvum / Q000166:cytology*; D005243:Feces / Q000469:parasitology; D033661:Oocysts / Q000737:chemistry* / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D011309:Preservation, Biological / Q000379:methods; D013010:Sonication; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors; D014867:Water
chemical_list: D014867:Water