Re-evaluation of commercially available enzyme-linked immunosorbent assay for the detection of Giardia lamblia and Cryptosporidium spp from stool specimens.
This study aimed to detect Giardia lamblia and Cryptosporidium spp infection from stool specimens. A total of 345 stool specimens were examined by microscopy (both direct smear and formalin concentration) and EIA techniques (ProSpecT Microplate Assay) for G. lamblia and Cryptosporidium spp. Of 73 tests positive for G. lamblia, 41(56.2%) were positive by microscopy, and 71(97.3%) were positive by EIA. Of 16 tests positive for Cryptosporidium spp, 5 (31.3%) were positive by microscopy, and 16(100%) were positive by EIA technique. The results demonstrate that this EIA method is quick, simple, and more sensitive than the microscopy method and should be used for the detection of G. lamblia and Cryptosporidium spp where the prevalence of these protozoan parasites is a public health problem.
Srijan
Apichai
A
Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences AFRIMS, US Army Medical Component, 315/6 Rajavithi Road, Phyathai, Bangkok 10400, Thailand. apichais@afrims.org
Wongstitwilairoong
Boonchai
B
Pitarangsi
Chittima
C
Serichantalergs
Oralak
O
Fukuda
C D
CD
Bodhidatta
Ladaporn
L
Mason
C J
CJ
D000818:Animals; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000469:parasitology; D003458:Cryptosporidium / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D016829:Giardia lamblia / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis* / Q000469:parasitology; D006801:Humans; D008853:Microscopy; D012680:Sensitivity and Specificity