title: Utility of the oestrogen-dependent vaginal candidosis murine model in evaluating the efficacy of various therapies against vaginal Candida albicans infection.
abstract: The efficacy of yogurt treatment against vaginal candidosis (VC) was examined using an oestrogen-dependent vaginal candidosis (EDVC) murine model. The EDVC mouse model was constructed by inoculating mice with viable Candida albicans cells under pseudo-oestrus conditions. Vaginal fungal burden in the various mouse groups was evaluated at several time points following the induction of VC. Untreated and yogurt-treated naïve mice exhibited background levels of VC (<6000 CFU per mouse). Candida albicans colonisation in untreated EDVC mice was significantly higher (P < 0.05) than that in yogurt-treated EDVC mice at days 20-30. Metronidazole-treated naïve mice developed persistent C. albicans vaginal colonisation at significantly lower levels (P < 0.05) than that in untreated or metronidazole-treated EDVC mice. Lactobacillus was only detected in the reproductive tracts of yogurt-treated naïve and EDVC mice. These findings suggest that the presence of Lactobacillus in the reproductive tract can suppress C. albicans growth and the antibiotics may predispose to VC.
authors.affiliation: Department of Biological Sciences, Hashemite University, Zarqa, Jordan. taqiwmohanad@yahoo.com
authors.forename: Mawieh
authors.initials: M
authors.lastname: Hamad
authors.forename: Enas
authors.initials: E
authors.lastname: Muta'eb
authors.forename: Qasem
authors.initials: Q
authors.lastname: Abu-Shaqra
authors.forename: Abeer
authors.initials: A
authors.lastname: Fraij
authors.forename: Khaled
authors.initials: K
authors.lastname: Abu-Elteen
authors.forename: Salem R
authors.initials: SR
authors.lastname: Yasin
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage; D002176:Candida albicans / Q000254:growth & development / Q000302:isolation & purification; D002181:Candidiasis, Vulvovaginal / Q000188:drug therapy / Q000628:therapy*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000254:growth & development / Q000302:isolation & purification; D008795:Metronidazole / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D014621:Vagina / Q000382:microbiology; D015014:Yogurt
chemical_list: D000890:Anti-Infective Agents; D008795:Metronidazole