title: Three multiplex assays for detection of Borrelia burgdorferi sensu lato and Borrelia miyamotoi sensu lato in field-collected Ixodes nymphs in North America.
abstract: Two hundred fifty New Jersey field-collected Ixodes scapularis Say ticks and 17 Colorado Ixodes spinipalpis Hadwen & Nuttall ticks were tested using three separate multiplex real-time polymerase chain reaction (PCR) assays. One assay targets the rrs-rrlA IGS region of Borrelia spp. to detect Borrelia burgdorferi sensu lato (s.l.) and Borrelia miyamotoi s.l. The second assay targets the ospA region of B. burgdorferi s.l. to detect B. burgdorferi sensu stricto (s.s.), Borrelia bissettii, and Borrelia andersonii. The final assay targets the glpQ region of B. miyamotoi s.l. to differentiate B. miyamotoi LB-2001 and Borrelia lonestari. A testing scheme combining these tests yielded 18% of tested I. scapularis ticks surveyed from New Jersey positive for B. burgdorferi s.s., 3.2% I. scapularis ticks positive for B. miyamotoi LB-2001, and 41.2% I. spinipalpis ticks positive for B. bissettii surveyed from Colorado.
authors.affiliation: Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80522, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: Ullmann
authors.forename: E S
authors.initials: ES
authors.lastname: Gabitzsch
authors.forename: T L
authors.initials: TL
authors.lastname: Schulze
authors.forename: N S
authors.initials: NS
authors.lastname: Zeidner
authors.forename: J
authors.initials: J
authors.lastname: Piesman
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001898:Borrelia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D025065:Borrelia burgdorferi / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry; D005408:Flagellin / Q000235:genetics; D018884:Ixodes / Q000382:microbiology*; D008193:Lyme Disease / Q000382:microbiology; D008969:Molecular Sequence Data; D009656:North America; D009758:Nymph / Q000382:microbiology; D010727:Phosphoric Diester Hydrolases / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012061:Relapsing Fever / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C064192:flaB1 protein, bacteria; D005408:Flagellin; D010727:Phosphoric Diester Hydrolases; C087921:glpQ protein, Bacteria