Oral vaccination of mice against rodent malaria with recombinant Lactococcus lactis expressing MSP-1(19).
OBJECTIVE
To construct the recombinant Lactococcus lactis as oral delivery vaccination against malaria.


METHODS
The C-terminal 19-ku fragments of MSP1 (MSP-1(19)) of Plasmodium yoelii 265-BY was expressed in L. lactis and the recombinant L. lactis was administered orally to BALB/c and C57BL/6 mice. After seven interval vaccinations within 4 wk, the mice were challenged with P. yoelii 265-BY parasites of erythrocytic stage. The protective efficacy of recombinant L. lactis was evaluated.


RESULTS
The peak parasitemias in average for the experiment groups of BALB/c and C57BL/6 mice were 0.8+/-0.4% and 20.8+/-26.5%, respectively, and those of their control groups were 12.0+/-0.8% and 60.8+/-9.6%, respectively. None of the BALB/c mice in both experimental group and control group died during the experiment. However, all the C57BL/6 mice in the control group died within 23 d and all the vaccinated mice survived well.


CONCLUSIONS
The results imply the potential of recombinant L. lactis as oral delivery vaccination against malaria.
Zhang
Zhi-Hong
ZH
Department of Biochemistry, Fudan University, 220 Han Dan Road, Shanghai 200433, China.
Jiang
Pei-Hong
PH
Li
Ning-Jun
NJ
Shi
Mi
M
Huang
Weida
W
D000284:Administration, Oral; D000818:Animals; D006801:Humans; D013294:Lactococcus lactis / Q000276:immunology*; D008288:Malaria / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D017780:Malaria Vaccines / Q000008:administration & dosage* / Q000627:therapeutic use; D020066:Merozoite Surface Protein 1 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018512:Parasitemia; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D010967:Plasmodium yoelii / Q000276:immunology; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D012376:Rodent Diseases / Q000276:immunology / Q000517:prevention & control*; D015996:Survival Rate; D014611:Vaccination
D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1; D010446:Peptide Fragments; D021122:Protein Subunits; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins