title: Functional analysis of the white gene of Drosophila by P-factor-mediated transformation.
abstract: A 12-kb DNA segment spanning the white (w) locus of Drosophila has been inserted into a P-transposon vector and used for P-factor-mediated germ-line transformation. Several red-eyed transformants were recovered which complement the white mutant phenotype. Analysis of the eye pigments and the interaction with the zeste mutation indicates that the w gene inserted at several new chromosomal sites is expressed normally. The tissue-specific accumulation of w transcripts, as studied by in situ hybridization to tissue sections, is the same in transformant and wild-type larvae. This indicates that all the genetic information specified by the w locus is contained within this 12-kb segment of DNA. By secondary mobilization it was shown that the w sequences have been inserted as a functional P(w) transposon which is capable of further transposition.
authors.affiliation: Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
authors.forename: W J
authors.initials: WJ
authors.lastname: Gehring
authors.forename: R
authors.initials: R
authors.lastname: Klemenz
authors.forename: U
authors.initials: U
authors.lastname: Weber
authors.forename: U
authors.initials: U
authors.lastname: Kloter