Urinary corticosterone levels in mice in response to intraperitoneal injections with saline.
The concept of refinement is an important issue in the field of laboratory animal science. Refinement-based research aims to improve animal welfare, to increase the reliability of experimental outcome, and to diminish variation. In search of refinement of experimental techniques, this study investigated whether urinary corticosterone can be used as a noninvasive measure of acute stress in mice.
Meijer
Margot K
MK
Department of Animals, Science, and Society, Utrecht University, The Netherlands. m.k.meijer@vet.uu.nl
Lemmens
Arnoldina G
AG
Van Zutphen
Bert F M
BF
Baumans
Vera
V
D000818:Animals; D000830:Animals, Laboratory; D019540:Area Under Curve; D015415:Biomarkers / Q000652:urine; D003345:Corticosterone / Q000652:urine*; D003404:Creatinine / Q000652:urine; D005260:Female; D007274:Injections, Intraperitoneal / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011863:Radioimmunoassay / Q000662:veterinary; D012376:Rodent Diseases / Q000175:diagnosis / Q000652:urine*; D012965:Sodium Chloride / Q000008:administration & dosage* / Q000494:pharmacology; D013312:Stress, Physiological / Q000175:diagnosis / Q000652:urine / Q000662:veterinary*
D015415:Biomarkers; D012965:Sodium Chloride; D003404:Creatinine; D003345:Corticosterone