title: Biochemical characterization and preliminary X-ray crystallographic study of the domains of human ZBP1 bound to left-handed Z-DNA.
abstract: ZBP1 is involved in host responses against cellular stresses, including tumorigenesis and viral infection. Structurally, it harbors two copies of the Zalpha domain containing the Zalpha motif, at its N terminus. Here, we attempted to characterize the Z-DNA binding activities of two Zalpha domains in the human ZBP1, hZalpha(ZBP1) and hZbeta(ZBP1), using circular dichroism (CD). Our results indicated that both hZalpha(ZBP1) and hZbeta(ZBP1) are viable Z-DNA binders, and their binding activities are comparable to those of previously-established Zalpha domains. Additionally, we crystallized hZbeta(ZBP1) in a complex with Z-DNA, d(TCGCGCG)2. The crystal diffracted to 1.45 angstroms, and belongs to the P2(1)2(1)2(1) space group, with the unit-cell parameters: a = 29.53 angstroms, b = 58.25 angstroms, and c = 88.61 angstroms. The delineation of this structure will provide insight into the manner in which diverse Zalpha motifs recognize Z-DNA.
authors.affiliation: Department of Molecular Cell Biology, Center for Molecular Medicine, SBRI, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Ha
authors.forename: Dong
authors.initials: D
authors.lastname: Van Quyen
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Hwang
authors.forename: Doo-Byoung
authors.initials: DB
authors.lastname: Oh
authors.forename: Bernard A
authors.initials: BA
authors.lastname: Brown
authors.forename: Seon Min
authors.initials: SM
authors.lastname: Lee
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Park
authors.forename: Jin-Hyun
authors.initials: JH
authors.lastname: Ahn
authors.forename: Kyeong Kyu
authors.initials: KK
authors.lastname: Kim
authors.forename: Yang-Gyun
authors.initials: YG
authors.lastname: Kim
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D043542:DNA, Z-Form / Q000737:chemistry*; D004268:DNA-Binding Proteins; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins
chemical_list: D043542:DNA, Z-Form; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D016601:RNA-Binding Proteins; C485731:ZBP1 protein, human