title: Evaluation of the CombiChip Mycobacteria Drug-Resistance detection DNA chip for identifying mutations associated with resistance to isoniazid and rifampin in Mycobacterium tuberculosis.
abstract: The CombiChip Mycobacteriatrade mark Drug-Resistance Detection DNA chip, recently developed by GeneIn (Pusan, South Korea), is an oligonucleotide microchip coupled with polymerase chain reaction for the detection of mutations associated with resistance to isoniazid (INH) and rifampin (RIF). This oligonucleotide chip was compared with DNA sequencing and phenotypic drug susceptibility testing with 69 INH- and/or RIF-resistant and 27 all tested drug-susceptible Mycobacterium tuberculosis isolates. Two selected codons (the katG codon 315 and inhA15) allowed identification of 84.1% of INH-resistant isolates and 100% of RIF resistance were detected by screening for 7 codons: rpoB511, rpoB513, rpoB516, rpoB522, rpoB526, rpoB531, and rpoB533. The overall specificity of this oligonucleotide chip for detecting INH and RIF resistance were 100 and 95.3%, respectively. This level of sensitivity and specificity is concordant with that from the determination of M. tuberculosis drug resistance by DNA sequencing. This oligonucleotide chip is a rapid and reliable genotypic method capable of detecting multiple mutations associated with INH and RIF resistance simultaneously in a single microchip slide.
authors.affiliation: Department of Clinical Pathology, College of Medicine, The Catholic University of Korea, St. Vincent's Hospital, Suwon 442-723, South Korea.
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Yeon-Joon
authors.initials: YJ
authors.lastname: Park
authors.forename: Eunsil
authors.initials: E
authors.lastname: Song
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Jang
authors.forename: Cheolmin
authors.initials: C
authors.lastname: Kim
authors.forename: Jinyoung
authors.initials: J
authors.lastname: Yoo
authors.forename: Seok-Jin
authors.initials: SJ
authors.lastname: Kang
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D003062:Codon; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D003062:Codon; D004269:DNA, Bacterial; D007538:Isoniazid; D012293:Rifampin