title: ADAM12 is a four-leafed clover: the excised prodomain remains bound to the mature enzyme.
abstract: The ADAMs (a disintegrin and metalloprotease) comprise a family of multidomain proteins with metalloprotease, cell adhesion, and signaling activities. Human ADAM12, which is implicated in diseases such as cancer, is expressed in two splice forms, the transmembrane ADAM12-L and the shorter and soluble ADAM12-S. ADAM12 is synthesized as a zymogen with the prodomain keeping the metalloprotease inactive through a cysteine-switch mechanism. Maturation and activation of the protease involves the cleavage of the prodomain in the trans-Golgi or possibly at the cell surface by a furin-peptidase. The aim of the present study was to determine the fate of the prodomain following furin cleavage. Here we demonstrate that, following cleavage of the human ADAM12-S prodomain in the trans-Golgi by a furin-peptidase, the prodomain remains non-covalently associated with the mature molecule. Accordingly, both the 68-kDa mature form of ADAM12-S and the 25-kDa prodomain could be detected using domain-specific antisera in immunoprecipitation and Western blot analyses of human serum ADAM12 and purified recombinant human ADAM12. Using electron microscopy after negative staining we have furthermore obtained the first visualization of a full-length ADAM molecule, human ADAM12-S, and report that it appears to be a compact clover composed of four globular domains, one of which is the prodomain. Finally, our data demonstrate that the presence of the metalloprotease domain appears to be sufficient for the prodomain to remain associated with the mature ADAM12-S. Thus, we conclude that the prodomain of human ADAM12-S is an integral domain of the mature molecule and as such might have specific biological functions in the extracellular space.
authors.affiliation: Institute of Molecular Pathology, University of Copenhagen, Frederik V's vej 11, 2100 Copenhagen, Denmark. ullaw@pai.ku.dk
authors.forename: Ulla M
authors.initials: UM
authors.lastname: Wewer
authors.forename: Matthias
authors.initials: M
authors.lastname: Mörgelin
authors.forename: Peter
authors.initials: P
authors.lastname: Holck
authors.forename: Jonas
authors.initials: J
authors.lastname: Jacobsen
authors.forename: Magnus C
authors.initials: MC
authors.lastname: Lydolph
authors.forename: Anders H
authors.initials: AH
authors.lastname: Johnsen
authors.forename: Marie
authors.initials: M
authors.lastname: Kveiborg
authors.forename: Reidar
authors.initials: R
authors.lastname: Albrechtsen
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D000072199:ADAM12 Protein; D017398:Alternative Splicing; D015153:Blotting, Western; D004789:Enzyme Activation; D004792:Enzyme Precursors; D045683:Furin / Q000378:metabolism*; D006056:Golgi Apparatus; D006801:Humans; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D045726:Metalloproteases / Q000378:metabolism; D008854:Microscopy, Electron
chemical_list: D004792:Enzyme Precursors; D008565:Membrane Proteins; D045726:Metalloproteases; D045683:Furin; D051722:ADAM Proteins; D000072199:ADAM12 Protein; C000606480:ADAM12 protein, human