title: Prestressed F-actin networks cross-linked by hinged filamins replicate mechanical properties of cells.
abstract: We show that actin filaments, shortened to physiological lengths by gelsolin and cross-linked with recombinant human filamins (FLNs), exhibit dynamic elastic properties similar to those reported for live cells. To achieve elasticity values of comparable magnitude to those of cells, the in vitro network must be subjected to external prestress, which directly controls network elasticity. A molecular requirement for the strain-related behavior at physiological conditions is a flexible hinge found in FLNa and some FLNb molecules. Basic physical properties of the in vitro filamin-F-actin network replicate the essential mechanical properties of living cells. This physical behavior could accommodate passive deformation and internal organelle trafficking at low strains yet resist externally or internally generated high shear forces.
authors.affiliation: Department of Physics and Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Gardel
authors.forename: F
authors.initials: F
authors.lastname: Nakamura
authors.forename: J H
authors.initials: JH
authors.lastname: Hartwig
authors.forename: J C
authors.initials: JC
authors.lastname: Crocker
authors.forename: T P
authors.initials: TP
authors.lastname: Stossel
authors.forename: D A
authors.initials: DA
authors.lastname: Weitz
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D003285:Contractile Proteins / Q000737:chemistry* / Q000378:metabolism*; D064448:Filamins; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits
chemical_list: D000199:Actins; D003285:Contractile Proteins; C578662:FLNB protein, human; D064448:Filamins; D008840:Microfilament Proteins