Isolation of E. coli O157:H7 and non-O157 STEC in different matrices: review of the most commonly used enrichment protocols.
OBJECTIVE
To review and characterize the enrichment protocols used for detecting all Shiga-Toxin producing Escherichia coli (STEC) from different matrices.


RESULTS
Firstly, the frequency distribution of the factors characterizing the enrichment protocols is described; secondly, a multiple correspondence analysis is performed to display profiles of association of these factors, and thirdly, published results concerning the relative performances of the protocols are summarized. Trypticase Soy Broth (TSB) is reported as the most frequently used enrichment broth. More often, one antibiotic is added in enrichment broths and these broths are incubated for a duration of 16-24 h at 35-37 degrees C. It also appears that the incubation temperature does not seem to be related to the type of serogroup looked for and that antibiotics are used regardless of the matrix analysed. Finally, results relating to the enrichment protocol efficacy are rare and differ from one study to another.


CONCLUSIONS
Statistical studies must be conducted so as to assess the efficacy of the main enrichment protocols investigated in this study.


CONCLUSIONS
This study reviews the most commonly used enrichment protocols and highlights the lack of results as to their relative efficacy.
Vimont
A
A
Unité de Microbiologie Alimentaire et Prèvisionnelle, Ecole Nationale Vétérinaire de Lyon, Marcy l'Etoile, France.
Vernozy-Rozand
C
C
Delignette-Muller
M-L
ML
D015169:Colony Count, Microbial / Q000379:methods; D003470:Culture Media / Q000737:chemistry*; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media