title: Catellicoccus marimammalium gen. nov., sp. nov., a novel Gram-positive, catalase-negative, coccus-shaped bacterium from porpoise and grey seal.
abstract: Two strains of an unknown Gram-positive, catalase-negative, facultatively anaerobic, coccus-shaped bacterium, originating from a porpoise and a grey seal, were characterized using phenotypic, biochemical and molecular phylogenetic methods. Chemical studies revealed the presence of a cell-wall murein based on L-lysine (type L-lys-gly-D-Asp) and a DNA G+C content of 38 mol%. Comparative 16S rRNA gene sequencing showed that this unidentified coccus-shaped organism formed a hitherto unknown subline closely related to, albeit distinct from, a number of other catalase-negative genera which included Enterococcus, Melissococcus, Tetragenococcus and Vagococcus. Other known Gram-positive, catalase-negative taxa were more distantly related. Tree-branching considerations and sequence divergence values of >6% with recognized taxa were indicative of this novel bacterium representing a separate genus. Based on phenotypic and phylogenetic evidence, it is proposed that this unknown bacterium, recovered from a porpoise and a grey seal, be classified as a novel genus and species, Catellicoccus marimammalium gen. nov., sp. nov. The type strain is M35/04/3T (=CCUG 49459T=CIP 108575T).
authors.affiliation: Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lawson
authors.affiliation: 54 Parkway, Crowthorne, Reading RG45 6EW, UK.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Collins
authors.affiliation: Culture Collection, Department of Clinical Bacteriology, University of Göteborg, S-413 46 Göteborg, Sweden.
authors.forename: Enevold
authors.initials: E
authors.lastname: Falsen
authors.affiliation: SAC Veterinary Services, Inverness IV2 4JZ, UK.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Foster
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D015373:Bacterial Typing Techniques; D002374:Catalase / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D006095:Gram-Positive Cocci / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D019576:Porpoises / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D046048:Seals, Earless / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002374:Catalase