title: Dominulin A and B: two new antibacterial peptides identified on the cuticle and in the venom of the social paper wasp Polistes dominulus using MALDI-TOF, MALDI-TOF/TOF, and ESI-ion trap.
abstract: Two new antibacterial peptides, denominated as Dominulin A and B, have been found on the cuticle and in the venom of females of the social paper wasp Polistes dominulus. The amino acidic sequence of the two peptides, determined by mass spectrometry, is INWKKIAE VGGKIL SSL for Dominulin A (MW = 1854 Da) and INWKKIAEIGKQVL SAL (MW = 1909 Da) for Dominulin B. Their presence on the cuticle was confirmed using MALDI-TOF by means of micro-extractions and direct analyses on body parts. The presence in the venom and the primary structure of the dominulins suggest their classification in the mastoparans, a class of peptides found in the venom of other Aculeate hymenoptera. Their antimicrobial action against Gram+ and Gram- bacteria fits in the range of the best natural antimicrobial peptides. Dominulins can represent an important defense of the colony of Polistes dominulus against microbial pathogens.
authors.affiliation: Centro Interdipartimentale di Spettrometria di Massa, Università di Firenze, Firenze, Italy. turillazzi@dbag.unifi.it
authors.forename: Stefano
authors.initials: S
authors.lastname: Turillazzi
authors.forename: Guido
authors.initials: G
authors.lastname: Mastrobuoni
authors.forename: Francesca R
authors.initials: FR
authors.lastname: Dani
authors.forename: Gloriano
authors.initials: G
authors.lastname: Moneti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pieraccini
authors.forename: Giancarlo
authors.initials: G
authors.lastname: la Marca
authors.forename: Gianluca
authors.initials: G
authors.lastname: Bartolucci
authors.forename: Brunella
authors.initials: B
authors.lastname: Perito
authors.forename: Duccio
authors.initials: D
authors.lastname: Lambardi
authors.forename: Vanni
authors.initials: V
authors.lastname: Cavallini
authors.forename: Leonardo
authors.initials: L
authors.lastname: Dapporto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000032:analysis / Q000494:pharmacology; D001412:Bacillus subtilis / Q000166:cytology / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods; D012867:Skin / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014862:Wasp Venoms / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology; D014863:Wasps / Q000378:metabolism*
chemical_list: D023181:Antimicrobial Cationic Peptides; D014862:Wasp Venoms; C510985:dominulin A; C510986:dominulin B