A rapid TLC autographic method for the detection of xanthine oxidase inhibitors and superoxide scavengers.
A new bioautographic assay suitable for the localization of xanthine oxidase inhibitors and superoxide radical scavengers present in a complex matrix is described. Enzyme activity is detected by reaction of superoxide radicals with nitroblue tetrazolium to form a blue formazan salt. Both activities can be differentiated using a non-enzymatic version of the autographic assay wherein superoxide is chemically generated.
Ramallo
I Ayelen
IA
Cátedra de Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.
Zacchino
Susana A
SA
Furlan
Ricardo L E
RL
D002855:Chromatography, Thin Layer / Q000379:methods*; D004791:Enzyme Inhibitors / Q000032:analysis* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000032:analysis* / Q000494:pharmacology; D013481:Superoxides / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism
D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D013481:Superoxides; D014969:Xanthine Oxidase