title: Induction of transforming growth factor-beta 1 on dentine pulp cells in different culture patterns.
abstract: Recent studies have documented that TGF-beta1 takes part in dental pulp tissue repair. Moreover, dental pulp cells have the potential to differentiate into odontoblast-like cells and produce reparative dentine in this process. However, the molecular mechanisms and potential interactions between TGF-beta1 and dental pulp cells are not clear due to the complexity of the pulp/dentine microenvironment. In this study, we investigated the induction of TGF-beta1 on the dental pulp cells in cell culture, tissue culture and three-dimensional culture patterns. These results demonstrated that TGF-beta1 significantly increased the proliferation of cells and activity of ALPase. Dental pulp cells cultured in the presence of TGF-beta1 formed mineralization nodules. In the organ culture, dental pulp cells treated with TGF-beta1 differentiated into odontoblast-like cells and formed a pulp-dentinal complex; and TGF-beta1 significantly induced synthesis of dentine relative proteins DSPP, DMP-1. The dental pulp cells share some characteristics of the odontoblast, such as a parallel arrangement with columnar form and a unilateral cell process. Together, these data indicate that TGF-beta1 can make dental pulp cells differentiated into odontoblast-like cells and form the pulp-dentinal complex. Moreover, these results suggest that TGF-beta1 is an important regulatory factor in odontoblast differentiation during tooth development and pulp repair.
authors.affiliation: Department of Oral and Maxillofacial Surgery, West China Stomatology College, Sichuan University, Chengdu 610041, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Nie
authors.forename: Weidong
authors.initials: W
authors.lastname: Tian
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xizhe
authors.initials: X
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Dong
authors.forename: Ming
authors.initials: M
authors.lastname: Jiang
authors.forename: Famin
authors.initials: F
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D001857:Bone Matrix / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D053773:Transforming Growth Factor beta1
chemical_list: D012333:RNA, Messenger; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1