Lack of association between the -403G/A promoter polymorphism in the human CCL5/RANTES chemokine gene in liver transplant outcome.
Chemokines play a major role in the inflammatory and immune responses that mediate allograft outcome. CCL5/RANTES expansion chemokine is potent eosinophil, monocyte, basophils and lymphocyte chemoattractant and has recently been studied in transplantation with discrepant results, but systemic concentrations have been correlated to liver graft survival and incidence of rejection. Recent studies revealed that a functional mutation at -403 in the promoter may have a significance for inflammatory and infectious immune responses. Our objective was to investigate CCL5/RANTES promoter polymorphism in rejection and graft survival in liver transplant. We examined the CCL5/RANTES polymorphism in a series of 218 liver transplants and 101 healthy Caucasian subjects. CCL5/RANTES genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). After comparing recipients (with acute rejection episodes versus without rejection) with the control population, we found no significant deviation in the distribution of the alleles or genotypes of CCL5/RANTES dimorphism in any comparison (P > 0.05). Indeed, 5 years allograft survival was 61.3% in recipients with the GG genotype against 58.8% in recipients with the GA and AA genotypes. These differences were also not statistically significant. In conclusion, human CCL5/RANTES gene promoter polymorphism does not seem to influence acute rejection development and allograft survival in liver recipients.
Botella
Carmen
C
Immunology Service, University Hospital Virgen Arrixaca, Murcia 30120, Spain.
Marín
Luis
L
Moya-Quiles
Rosa
R
Miras
Manuel
M
Sánchez-Bueno
Francisco
F
Minguela
Alfredo
A
Bermejo
Juan
J
Parrilla
Pascual
P
Alvarez-López
Rocío
R
Muro
Manuel
M
D000328:Adult; D001483:Base Sequence; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000235:genetics*; D019742:Chemokines, CC / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology; D006801:Humans; D016031:Liver Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D016896:Treatment Outcome
C483024:CCL5 protein, human; D018946:Chemokine CCL5; D019742:Chemokines, CC; D004247:DNA