title: [Soluble CD40 and soluble CD40L concentrations in the serum and the cerebrospinal fluid of patients with tick borne encephalitis and neuroborreliosis].
abstract: OBJECTIVE
  The interaction between CD40 and CD40L is essential in generating of an immunological response also intrathecally. The aim of the study was estimation of a concentration soluble form of CD40, CD40L (CD154) in the bacterial and viral inflammation of the central nervous system in two compartments - blood circulation and intrathecally, before and after the treatment.
  
  
  METHODS
  sCD40 and sCD40L were tested twice before and after treatment in pairs serum and CSF of 40 patients treated in the Dept. of Infectious Diseases and Neuroinfections. Patients were divided in two groups: (n=20) patients with tick borne encephalitis (TBE) (group I, n=20) and patients with neuroborreliosis in the form of lymphocytic meningitis (group II, n=20). ELISA assays were performed.
  
  
  RESULTS
  Significantly increased concentrations of sCD40, sCD40L in CSF (higher in neuroborreliosis) were measured. We found also an increased concentration of sCD40L in inflammatory CSF in both tested groups (in neuroboreliosis lasting also after 4 weeks of treatment), compared with the control group (below the detection limit in normal CSF).
  
  
  CONCLUSIONS
  Results of estimation of the sCD40 and sCD40L concentrations indicate their role in the intrathecal inflammation process of bacterial and viral etiology. The increased serum concentration of sCD40L in TBE and CD40 in neuroborreliosis indicate that peripheral activation of the immunological system persists after cessation of treatment and after the clinical recovery. The defense mechanisms are more pronounced in neuroborreliosis than in tick borne encephalitis.
authors.affiliation: Klinika Chorób Zakaźnych i Neuroinfekcji, Akademia Medyczna w Białymstoku, Białystok. zajkowsk@kki.pl
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Zajkowska
authors.forename: Maciej
authors.initials: M
authors.lastname: Kondrusik
authors.forename: Sławomir
authors.initials: S
authors.lastname: Pancewicz
authors.forename: Renata
authors.initials: R
authors.lastname: Swierzbińska
authors.forename: Sambor
authors.initials: S
authors.lastname: Grygorczuk
authors.forename: Teresa
authors.initials: T
authors.lastname: Hermanowska-Szpakowicz
mesh_terms: D000328:Adult; D019013:CD40 Antigens / Q000097:blood* / Q000134:cerebrospinal fluid*; D023201:CD40 Ligand / Q000097:blood* / Q000134:cerebrospinal fluid*; D004675:Encephalitis, Tick-Borne / Q000097:blood* / Q000134:cerebrospinal fluid*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007964:Leukocytosis / Q000097:blood; D020852:Lyme Neuroborreliosis / Q000097:blood* / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged
chemical_list: D019013:CD40 Antigens; D023201:CD40 Ligand