title: Differential progression of neonatal diethylstilbestrol-induced disruption of the hamster testis and seminal vesicle.
abstract: The synthetic estrogen diethylstilbestrol (DES) is now recognized as the prototypical endocrine disruptor. Using a hamster experimental system, we performed a detailed temporal assessment of how neonatal DES-induced disruption progresses in the testis compared to the seminal vesicle. Both morphological and Western blot analyses confirmed that neonatal DES exposure alters androgen responsiveness in the male hamster reproductive tract. We also determined that the disruption phenomenon in the male hamster is manifest much earlier in the seminal vesicle than in the testis and that testis disruption often occurs differently between the pair of organs in a given animal. In the neonatally DES-exposed seminal vesicle, histopathological effects included: (1) general atrophy, (2) lack of exocrine products, (3) epithelial dysplasia, (4) altered organization of stromal cells and extracellular matrix, and (5) striking infiltration with polymorphonuclear leukocytes. Also, the morphological disruption phenomenon in the seminal vesicle was accompanied by a range of up-regulation and down-regulation responses in the whole organ levels of various proteins.
authors.affiliation: Department of Biological Sciences, Wichita State University, 1845 Fairmount, Wichita, KS 67260-0026, United States. william.hendry@wichita.edu
authors.forename: William J
authors.initials: WJ
authors.lastname: Hendry
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Weaver
authors.forename: Teran R
authors.initials: TR
authors.lastname: Naccarato
authors.forename: Shafiq A
authors.initials: SA
authors.lastname: Khan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D006224:Cricetinae; D004054:Diethylstilbestrol / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D012669:Seminal Vesicles / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D013739:Testosterone / Q000097:blood; D013997:Time Factors
chemical_list: D052244:Endocrine Disruptors; D011506:Proteins; D013739:Testosterone; D004054:Diethylstilbestrol