Thyroid hormone action at the cellular, genomic and target gene levels.
Thyroid hormone (TH) plays important roles in metabolism, growth and differentiation. Thyroid hormone receptors (TRs) are ligand-regulatable transcription factors that bind both TH and DNA enhancer sequences in the promoter region of target genes where they can interact with co-repressor and co-activator complexes. These interactons, in turn, have consequent effects on transcription. This review describes studies on TH action from our laboratory examining the cellular localization and motility of TRs using green fluorescent fusion proteins, gene expression profiles of TH in WT and TRalpha and TRbeta KO mice, as well as general transcription factor and co-activator recruitment on the promoters of target genes by TH in chromatin immunoprecipitation assays.
Yen
Paul M
PM
Department of Medicine, Johns Hopkins Bayview Medical Center, 4940 Eastern Avenue Rm B114, Baltimore, MD, USA. pyen3@jhmi.edu
Ando
Shinichiro
S
Feng
Xu
X
Liu
Ying
Y
Maruvada
Padma
P
Xia
Xianmin
X
D000818:Animals; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008958:Models, Molecular; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D020033:Protein Isoforms / Q000235:genetics / Q000502:physiology; D011988:Receptors, Thyroid Hormone / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D013963:Thyroid Hormones / Q000378:metabolism* / Q000494:pharmacology; D014158:Transcription, Genetic
D020033:Protein Isoforms; D011988:Receptors, Thyroid Hormone; D013963:Thyroid Hormones