title: Prevalence of selected infectious disease agents in cats from Arizona.
abstract: The objective of this study was to use polymerase chain reaction (PCR) assays to determine the prevalence of Ehrlichia species, Anaplasma phagocytophilum, Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum' and Bartonella species from feral and relinquished cats in Phoenix and Nogales, Arizona. DNA from one or more of the organisms was amplified from 31 of 112 blood samples (27.7%). DNA consistent with Bartonella clarridgeiae 15 (13.4%), Bartonella henselae 14 (12.5%), 'Candidatus M haemominutum' 9 (8.0%), and M haemofelis 5 (4.5%) were detected. DNA of Ehrlichia species, Neorickettsia risticii, or A phagocytophilum was not amplified. Failure to amplify DNA of A phagocytophilum may relate to the absence of appropriate tick vectors. Failure to amplify Ehrlichia species DNA suggests that cats were not exposed, exposed but not infected, or infected but the DNA was not detected by the PCR assay used in this study. The Bartonella species and hemoplasma results suggest flea control should be maintained.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 300 West Drake Road, Fort Collins, CO 80523, USA. Jason.Eberhardt@colostate.edu
authors.forename: Jason M
authors.initials: JM
authors.lastname: Eberhardt
authors.forename: Karter
authors.initials: K
authors.lastname: Neal
authors.forename: Tom
authors.initials: T
authors.lastname: Shackelford
authors.forename: Michael R
authors.initials: MR
authors.lastname: Lappin
mesh_terms: D041081:Anaplasma phagocytophilum / Q000302:isolation & purification; D000711:Anaplasmataceae Infections / Q000662:veterinary; D000818:Animals; D001130:Arizona / Q000453:epidemiology; D001473:Bartonella / Q000302:isolation & purification; D001474:Bartonella Infections / Q000662:veterinary; D002371:Cat Diseases / Q000453:epidemiology* / Q000382:microbiology* / Q000469:parasitology; D002415:Cats; D004269:DNA, Bacterial / Q000032:analysis; D004536:Ehrlichia / Q000302:isolation & purification; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary*; D007303:Insect Vectors / Q000382:microbiology*; D008297:Male; D009174:Mycoplasma / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000662:veterinary; D041103:Neorickettsia risticii / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D005423:Siphonaptera / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial