title: The distribution pattern of myofibroblasts in the stroma of human bladder carcinoma depends on their invasiveness.
abstract: The presence of myofibroblasts has been elucidated in the stroma of neoplasm of various organs. In the present article, we studied the distribution of myofibroblasts in the stroma of bladder carcinoma. Twenty-five surgical resected bladder tumors (urothelial carcinoma, n = 21; combined urothelial carcinoma and adenocarcinoma, n = 2; sarcomatoid squamous cell carcinoma, n = 1; combined urothelial carcinoma and squamous cell carcinoma, n = 1) were selected and we evaluated the distribution of myofibroblasts using immunohistochemical, electron and immunoelectron microscopic techniques. Immunohistochemically, the distribution pattern of myofibroblasts in invasive and non-invasive carcinomas were predominantly fascicular and reticular forms, respectively. Moreover, myofibroblasts around bladder carcinoma cells were confirmed by electron microscope. Understanding the distribution pattern of myofibroblasts in the stroma of bladder carcinoma may provide available information about the presence of carcinoma invasion.
authors.affiliation: Department of Urology, Program of Tumor Biology and Regulation, Kochi Medical School, Kochi University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Shimasaki
authors.forename: N
authors.initials: N
authors.lastname: Kuroda
authors.forename: E
authors.initials: E
authors.lastname: Miyazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Toi
authors.forename: M
authors.initials: M
authors.lastname: Hiroi
authors.forename: H
authors.initials: H
authors.lastname: Enzan
authors.forename: T
authors.initials: T
authors.lastname: Shuin
mesh_terms: D000199:Actins / Q000032:analysis; D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D000368:Aged; D000369:Aged, 80 and over; D002148:Calmodulin-Binding Proteins / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D032389:Myocytes, Smooth Muscle / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D017154:Stromal Cells / Q000737:chemistry / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure
chemical_list: D000199:Actins; D002148:Calmodulin-Binding Proteins