title: New TEM-derived extended-spectrum beta-lactamase and its genomic context in plasmids from Salmonella enterica serovar derby isolates from Uruguay.
abstract: A small (8.2-kb) ColE1 plasmid encoding TEM-144 (a new beta-lactamase with a ceftazidimase profile) was sequenced by a gene-walking strategy. The bla(TEM) allele was carried on a Tn2 element, disrupting a Rom protein gene. TEM-144 differs from TEM-1 by two mutations (R164C and E240K) and from the ceftazidime-hydrolyzing TEM-91 by one mutation (T182M).
authors.affiliation: Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, 11600 Montevideo, Uruguay.
authors.forename: Rafael
authors.initials: R
authors.lastname: Vignoli
authors.forename: Nicolas F
authors.initials: NF
authors.lastname: Cordeiro
authors.forename: Virginia
authors.initials: V
authors.lastname: García
authors.forename: María Inés
authors.initials: MI
authors.lastname: Mota
authors.forename: Laura
authors.initials: L
authors.lastname: Betancor
authors.forename: Pablo
authors.initials: P
authors.lastname: Power
authors.forename: José A
authors.initials: JA
authors.lastname: Chabalgoity
authors.forename: Felipe
authors.initials: F
authors.lastname: Schelotto
authors.forename: Gabriel
authors.initials: G
authors.lastname: Gutkind
authors.forename: Juan A
authors.initials: JA
authors.lastname: Ayala
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D010957:Plasmids; D019779:Salmonella enterica / Q000201:enzymology* / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D001618:beta-Lactamases