The wheat chloroplast gene for CF(0) subunit I of ATP synthase contains a large intron.
The gene for CF(0) subunit I of ATP synthase has been located in wheat chloroplast DNA, between the genes for CF(0) subunit III and alpha subunit of CF(1). Nucleotide sequencing and analysis of RNA-DNA hybrids indicated that the gene is interrupted by an 823-bp intron which has boundaries similar to those previously described for the introns in protein-coding chloroplast genes of Euglena gracilis. The deduced amino acid sequence of CF(0) subunit I indicates a polypeptide of 183 amino acid residues. However, N-terminal amino acid sequencing of the mature spinach CF(0) subunit I suggests that the protein is synthesised with a N-terminal extension of 17 amino acid residues and is processed to give a protein of mol. wt. 19 001 of 166 amino acids residues. The mature CF(0) subunit I shows similarities in primary and predicted secondary structure to F(0) subunit b of Escherichia coli ATP synthase. A major transcript of 3.3 kb containing sequences from the genes for CF(0) subunit III, subunit I and CF(1) subunit alpha has been observed by RNA-DNA hybridisation.
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Botany School, University of Cambridge, Cambridge CB2 3EA, UK.
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Auffret
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AK
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Gray
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