Novel non-uniform distribution of serotonin transporter in the mouse hippocampus and neocortex revealed by N- and C-terminal domain-specific immunohistochemistry.
Serotonergic fibers have a general feature of extending diffusely throughout the brain and appear to innervate broad areas rather uniformly. The present study revealed marked regional difference in their immunoreactivities against serotonin transporter by using two antibodies that recognize either N- or C-terminal domain of the transporter. C-terminal-specific labeling was ubiquitous, whereas N-terminal-specific labeling was confined to hippocampal CA1 region, somatosensory cortex, and other areas, suggesting novel non-uniformity in the serotonergic system.
Shigematsu
Naoki
N
Clinical Pharmacokinetics, Division of Clinical Pharmacy, Department of Medico-Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Yamamoto
Kenji
K
Higuchi
Shun
S
Fukuda
Takaichi
T
D000818:Animals; D000906:Antibodies; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D019579:Neocortex / Q000378:metabolism*; D010446:Peptide Fragments / Q000032:analysis*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000378:metabolism*
D000906:Antibodies; D010446:Peptide Fragments; D050486:Serotonin Plasma Membrane Transport Proteins