Bcr interferes with beta-catenin-Tcf1 interaction.
The beta-catenin/Tcf complex is a downstream effector of the Wnt signalling pathway. It is a transcription complex, which activates gene expression and contributes to proliferation and tumor progression. Tcf1 in complex with beta-catenin is able to activate beta-catenin-dependent gene expression. We demonstrate that expressed Bcr is able to bind the transcription factor Tcf1 to disrupt the Tcf1/beta-catenin complex. Phosphorylation of Bcr by the tyrosine kinase pp60(src) can lead to dissociation of the transcriptionally inactive Bcr/Tcf1 complex. Thus two independent mechanisms may regulate Tcf/beta-catenin-mediated transcription via Bcr: binding to beta-catenin as we have previously shown and to Tcf1 as shown here.
Ress
Angelika
A
Institute of Medical Virology, University of Zurich, Gloriastrasse 30, CH-8006 Zurich, Switzerland.
Moelling
Karin
K
D000818:Animals; D002460:Cell Line; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000378:metabolism*; D006801:Humans; D046912:Multiprotein Complexes / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D051562:Proto-Oncogene Proteins c-bcr / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D051176:beta Catenin / Q000378:metabolism*
C496487:HNF1A protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D046912:Multiprotein Complexes; D051176:beta Catenin; D016392:Proto-Oncogene Proteins pp60(c-src); C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr