title: Expression of chaperonin 60 in the hippocampus of the streptozotocin diabetic rat.
abstract: Mitochondrial dysfunction and oxidative stress are implicated in the pathological changes observed in the diabetic central nervous system. In this study, using the streptozotocin-induced diabetic rat model we document for the first time the over-expression of a mitochondrial specific stress protein (chaperonin 60) in the CA1/CA3 regions of the diabetic hippocampus in the absence of neurodegeneration. The increase in expression of chaperonin 60 was not observed in the cohort treated with insulin, suggesting that the observed effect was not due to streptozotocin per se but due to the hyperglycaemic state induced by the diabetic state. The expression of chaperonin 60 was also positively correlated with a marker of mitochondrial oxidative stress (manganese superoxide dismutase). We suggest that chaperonin 60 could be an early event marker of mitochondrial dysfunction in the diabetic central nervous system and indeed be neuroprotective in the early stages of hyperglycaemic-induced oxidative stress.
authors.affiliation: Bioengineering, HortResearch, School of Science & Engineering, University of Waikato, Hamilton, New Zealand.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yuan
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Young
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Martinus
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods; D018834:Chaperonin 60 / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000008:administration & dosage; D007150:Immunohistochemistry / Q000379:methods; D007328:Insulin / Q000008:administration & dosage; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D018834:Chaperonin 60; D007004:Hypoglycemic Agents; D007328:Insulin; D013311:Streptozocin; D013482:Superoxide Dismutase