Optical detection of DNA conformational polymorphism on single-walled carbon nanotubes.
The transition of DNA secondary structure from an analogous B to Z conformation modulates the dielectric environment of the single-walled carbon nanotube (SWNT) around which it is adsorbed. The SWNT band-gap fluorescence undergoes a red shift when an encapsulating 30-nucleotide oligomer is exposed to counter ions that screen the charged backbone. The transition is thermodynamically identical for DNA on and off the nanotube, except that the propagation length of the former is shorter by five-sixths. The magnitude of the energy shift is described by using an effective medium model and the DNA geometry on the nanotube sidewall. We demonstrate the detection of the B-Z change in whole blood, tissue, and from within living mammalian cells.
Heller
Daniel A
DA
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Jeng
Esther S
ES
Yeung
Tsun-Kwan
TK
Martinez
Brittany M
BM
Moll
Anthonie E
AE
Gastala
Joseph B
JB
Strano
Michael S
MS
D016475:3T3 Cells; D000042:Absorption; D000327:Adsorption; D000818:Animals; D002413:Cations, Divalent / Q000737:chemistry; D002645:Chickens; D002942:Circular Dichroism; D004247:DNA / Q000097:blood / Q000737:chemistry*; D043542:DNA, Z-Form / Q000097:blood / Q000737:chemistry*; D005453:Fluorescence; D008433:Mathematics; D008628:Mercury / Q000032:analysis; D051379:Mice; D008958:Models, Molecular; D018482:Muscle, Skeletal / Q000737:chemistry; D037742:Nanotubes, Carbon; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D002413:Cations, Divalent; D043542:DNA, Z-Form; D037742:Nanotubes, Carbon; D009838:Oligodeoxyribonucleotides; D004247:DNA; D008628:Mercury