[Effect of Coeloglossum. viride var. bracteatum extract on oxidation injury in sub-acute senescent model mice].
OBJECTIVE
To study the anti-aging effects of Coeloglossum. viride (L) Hartm. var. bracteatum (Willd) Richter extract (CE) on senescent model mice induced by D-galactose and sodium nitrite.


METHODS
After one week of accommodation, 60 female NIH mice were divided into six groups with 10 mice in each group: normal control group, aging model group, Piracetam (positive control) group [300 mg/ (kg x d)], and CE reatment groups [2.5, 5, and 10 mg/ (kg x d)]. Mice in aging model group, Piracetam group, and CE treatment group were consecutively intraperitoneally injected with D-galactose [120 mg/ (kg x d)] and sodium nitrite [90 mg/ (kg x d)] for 60 days. From day 47, mice in Piracetam group and CE treatment group were po given Piracetam 300 mg/ (kg x d) or CE 2.5, 5, and 10 mg/ (kg x d). Mice in normal control group and aging model group were po given saline. The drug administration lasted for 14 days. Water maze test was performed to evaluate the learning and memory function in the mice. The activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA), and the activities of adenosinetriphosphatase (ATPase), and monoamine-oxidase (MAO) in brain tissue were measured.


RESULTS
The latencies in water maze test in aging model group mice were significantly longer than in normal control group (P < 0.01, P < 0.05), and the number of errors increased (P < 0.05). In aging model group mice, the activities of SOD, Na+K(+)-ATPase, and Ca2+Mg(2+)-ATPase decreased (P < 0.01, P < 0.05), while the content of MDA and the activities of MAO-A and MAO-B increased (P < 0.01). Piracetam [300 mg/ (kg x d), po] and CE [2.5, 5, 10 mg/ (kg x d), po] ameliorated the above changes in aging model mice.


CONCLUSIONS
CE may improve the memory dysfunction induced by consecutive injection of D-galactose and sodium nitrite,and has nootropic and antiaging effects.
Zhang
Dan
D
Department of Pharmacology, Institute of Materia Medica, CAMS and PUMC, Beijing 100050, China.
Zhang
Jian-jun
JJ
D000375:Aging / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D001921:Brain / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005690:Galactose / Q000633:toxicity; D007859:Learning Disabilities / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008995:Monoamine Oxidase / Q000378:metabolism; D029595:Orchidaceae; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology; D012977:Sodium Nitrite / Q000633:toxicity; D013482:Superoxide Dismutase / Q000378:metabolism
D010936:Plant Extracts; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D008995:Monoamine Oxidase; D012977:Sodium Nitrite; D005690:Galactose