DNA sequences acting as binding sites for NM23/NDPK proteins in melanoma M14 cells.
We isolated and analyzed by chromatin immunoprecipitation (ChIP) in viable M14 cells DNA sequences bound to the antimetastatic protein nucleoside diphosphate kinase (NM23/NDPK) to shed some light on the nuclear functions of this protein and on the mechanism by which it acts in development and cancer. We assessed the presence of selected sequences from promoters of platelet-derived growth factor A (PDGF-A), c-myc, myeloperoxidase (MPO), CD11b, p53, WT1, CCR5, ING1, and NM23-H1 genes in the cross-linked complexes. Quantitative PCR (Q-PCR) showed a substantial enrichment of the correlated oncosuppressor genes p53, WT1, ING1, and NM23-H1 in the immunoprecipitated (IP) DNA. This suggests that NM23/NDPK binding is involved in the transcription regulation of these genes. These results reveal new interactions that should help us to disclose the antimetastatic mechanism of NM23.
Cervoni
Laura
L
Dipartimento di Scienze Biochimiche A. Rossi Fanelli, Università di Roma La Sapienza, Italy.
Egistelli
Lorenza
L
Eufemi
Margherita
M
Scotto d'Abusco
Anna
A
Altieri
Fabio
F
Lascu
Ioan
I
Turano
Carlo
C
Giartosio
Anna
A
D001483:Base Sequence / Q000235:genetics; D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry / Q000302:isolation & purification; D047369:Chromatin Immunoprecipitation / Q000379:methods; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics*; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D008969:Molecular Sequence Data; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism; D009942:Organometallic Compounds / Q000737:chemistry*; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D002843:Chromatin; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009942:Organometallic Compounds; D010982:Platelet-Derived Growth Factor; D016271:Proto-Oncogene Proteins c-myc; D014157:Transcription Factors; C075278:platelet-derived growth factor A; C487125:potassium dichloro(ethylenediamine-N,N'-di-3-propionate)ruthenium(III); C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase