title: Epithelium and bacteria in periapical lesions.
abstract: OBJECTIVE
  The purpose of this study was to evaluate 50 human periapical lesions for bacteria and epithelium in a case study in dental practice.
  
  
  METHODS
  Specimens were obtained from the extraction of 50 untreated teeth that had lesions attached to their apices. The specimens were histologically evaluated using serial sections.
  
  
  RESULTS
  Bacteria were found in all teeth, colonizing necrotic tissue in the main canal, dentinal tubules, or apical ramifications, and in the body of the periapical lesion in 18 abscesses or cysts. Twenty-one lesions were epithelialized; 14 abscesses, 20 granulomas, and 16 cysts were distinguished. In 18 root canals inflamed tissue was found in the apical part of the canal. A single foramen was present in 13 cases while apical ramifications were found in 37 cases.
  
  
  CONCLUSIONS
  Granulomas were most common, and most epithelialized lesions were cysts. Bacteria were only detected periapically in abscesses or cysts. Inflamed tissue was present in the apical root canal in one third of cases.
authors.affiliation: dricucci@libero.it
authors.forename: Domenico
authors.initials: D
authors.lastname: Ricucci
authors.forename: Elizeu A
authors.initials: EA
authors.lastname: Pascon
authors.forename: Thomas R Pitt
authors.initials: TR
authors.lastname: Ford
authors.forename: Kaare
authors.initials: K
authors.lastname: Langeland
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003238:Connective Tissue / Q000473:pathology; D003786:Dental Pulp Cavity / Q000382:microbiology; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D004848:Epithelium / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D009504:Neutrophils; D010485:Periapical Periodontitis / Q000382:microbiology* / Q000473:pathology*; D011842:Radicular Cyst / Q000382:microbiology / Q000473:pathology*; D019227:Tooth Apex / Q000473:pathology