Biologically inspired sensing: infrared spectroscopic analysis of cell responses to an inhalation health hazard.
This work describes the development of a biologically based sensing technique to quantify chemical agents that pose inhalation health hazards. The approach utilizes cultured epithelial cells (A549 human type II pneumocytes) of the lung, exposed to potential toxins and monitored through the noninvasive means of infrared spectroscopy to quantify changes to cell physiology and function. Cell response to Streptolysin O, a cholesterol-binding cytolysin, is investigated here. Infrared spectra display changes in cell physiology indicative of membrane damage, altered proteins, and some nucleic acid damage. Methods to improve cell adhesion through modification of support surface properties are detailed. This spectroscopic approach not only provides a robust means to detect potential toxins but also provides information on modes of damage and mechanisms of cellular response.
Riley
Mark R
MR
Department of Agricultural and Biosystems Engineering, University of Arizona, Tucson, Arizona 85721, USA. riley@ag.arizona.edu
DeRosa
Diana
D
Blaine
Jeanette
J
Potter
B G
BG
Lucas
Pierre
P
Le Coq
David
D
Juncker
Christophe
C
Boesewetter
Dianne E
DE
Collier
Jayne M
JM
Boussard-Plédel
Catherine
C
Bureau
Bruno
B
D001426:Bacterial Proteins / Q000633:toxicity; D001681:Biological Assay; D015374:Biosensing Techniques / Q000295:instrumentation; D002448:Cell Adhesion; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D019570:Inhalation Exposure / Q000517:prevention & control; D008168:Lung / Q000166:cytology; D013055:Spectrophotometry, Infrared / Q000379:methods; D013301:Streptolysins / Q000633:toxicity*; D013499:Surface Properties
D001426:Bacterial Proteins; D013301:Streptolysins; C025964:streptolysin O