Angiogenesis facilitated by autologous whole bone marrow stem cell transplantation for Buerger's disease.
We hypothesized that angiogenesis can be triggered by autologous whole bone marrow stem cell transplantation. Twenty-seven patients (34 lower limbs) with Buerger's disease, who were not candidates for surgical revascularization or radiologic intervention, were enrolled in this study. Six sites of the tibia bone were fenestrated using a 2.5-mm-diameter screw under fluoroscopic guidance. Clinical status and outcome were determined using the "Recommended Standards for Reports." To mobilize endothelial progenitor cells (EPCs) from bone marrow, recombinant human granulocyte colony-stimulating factor (r-HuG-CSF) was injected subcutaneously as a dose of 75 microg, preoperatively. During the follow-up period (19.1 +/- 3.5 months), one limb showed a markedly improved outcome (+3), and 26 limbs showed a moderately improved outcome (+2). Thirteen limbs among 17 limbs with nonhealing ulcers healed. Postoperative angiograms were obtained for 22 limbs. Two limbs showed marked (+3), five limbs moderate (+2), and nine limbs slight (+1) collateral development. However, six limbs showed no collateral development (0). Peripheral blood and bone marrow samples were analyzed for CD34 and CD133 molecules to enumerate potential EPCs, and EPC numbers were found to be increased in peripheral blood and in bone marrow after r-HuG-CSF injection. In conclusion, the transplantation of autologous whole BMCs by fenestration of the tibia bone represents a simple, safe, and effective means of inducing therapeutic angiogenesis in patients with Buerger's disease.
Kim
Dong-Ik
DI
Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Kangnamku, Seoul 135-710, Korea. dikim@smc.samsung.co.kr
Kim
Mi-Jung
MJ
Joh
Jin-Hyun
JH
Shin
Sung-Wook
SW
Do
Young-Soo
YS
Moon
Ji-Young
JY
Kim
Na-Ri
NR
Lim
Joung-Eun
JE
Kim
Ae-Kyeong
AK
Eo
Hyun-Seon
HS
Kim
Byung-Soo
BS
Cho
Seung-Woo
SW
Yang
Seung-Hye
SH
Park
Chan-Jeoung
CJ
Shim
Jong-Sup
JS
D000328:Adult; D001854:Bone Marrow Cells; D005260:Female; D006801:Humans; D008297:Male; D018919:Neovascularization, Physiologic; D033581:Stem Cell Transplantation; D013919:Thromboangiitis Obliterans / Q000628:therapy*; D014182:Transplantation, Autologous; D016896:Treatment Outcome