title: Fluorescence quenching method for the determination of sodium carboxymethyl cellulose with acridine yellow or acridine orange.
abstract: In near neutral to weak basic media, sodium carboxymethyl cellulose (NaCMC) will dissociate to become a macro polymeric anion, which can react with acridine yellow (AY) or acridine orange (AO) to form an ion-association complex resulting in fluorescence quenching of the acridine dyes. The maximum fluorescence quenching wavelength is 505 nm (lambda(ex)=440 nm) for AY system and 530 nm (lambda(ex)=493 nm) for AO system, respectively. The fluorescence quenching values (DeltaF) are directly proportional to the concentrations of NaCMC and the linear ranges are 20.0-4000 microg/L for AY system and 20.0-7000 microg/L for AO system, separately. This method has high sensitivity and the detection limits for NaCMC are 58.0 microg/L (AY system) and 157.2 microg/L (AO system). The effects of coexistent substance have been investigated, and the results show that this method has a relatively good selectivity. A fluorescence quenching method for the determination of NaCMC based on the ion-association reactions of CMC polymeric anion with a basic acridine dye was developed. The method is sensitive, simple and fast.
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing 400715, China. liusp@swnu.edu.cn
authors.forename: Shao Pu
authors.initials: SP
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: Sa
authors.forename: Xiao Li
authors.initials: XL
authors.lastname: Hu
authors.forename: Ling
authors.initials: L
authors.lastname: Kong
mesh_terms: D000165:Acridine Orange / Q000494:pharmacology*; D000609:Aminoacridines / Q000494:pharmacology*; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000494:pharmacology*; D008956:Models, Chemical; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
chemical_list: D000609:Aminoacridines; D005456:Fluorescent Dyes; D000165:Acridine Orange; D002266:Carboxymethylcellulose Sodium; C025902:acridine yellow