Chemical characteristics and immuno-stimulating properties of biopolymers extracted from Acanthopanax sessiliflorus.
During our search for macrophage stimulating compounds from medicinal plants, we isolated biopolymers from Acanthopanax sessiliflorus. Isolated fraction AS-5 showed maximum potential, and stimulated lysosonal enzymatic activity by 230% at 300 microg/ml. The nitric oxide (NO) producing ability of AS-5 100 microg/ml was 58 microM when treated with interferon-gamma and lipopolysaccharide 20 micro/ml. The lymphocyte proliferating effects of isolated biopolymer fractions were also investigated. Highest lymphoproliferative activity (a 2.8-fold enhancement compared to salines treated group was exhibited by AS-3 at 200 micro/ml followed by AS-5 and AS-6. The AS-3 fraction stimulated only T-lymphocytes and had little or no effect on B-lymphocyte proliferation. Partially methylated alditol acetates were prepared to elucidate the glycosyl linkage-compositions of the AS-3 and AS-5 biopolymers, and were analyzed by GC-MS. The AS-3 and AS-5 biopolymer fractions were found to contain 2,3,4-tri-O-methyl-D-glucitol, 2,3,4-tri-O-methyl-D-galacitol 3,4,6-tri-O-methyl-galacitol, 2-O-methyl-arabinitol and 2,4,6-tri-O-methyl-D-glucitol, 2,3,6-tri-O-methyl-D-galacitol linkages, respectively.
Jeong
Sang-Chul
SC
Department of Biotechnology, Daegu University, Gyungsan, Gyungbuk 712-714, Korea.
Jeong
Young-Tae
YT
Yang
Byung-Keun
BK
Song
Chi-Hyun
CH
D000818:Animals; D001704:Biopolymers / Q000737:chemistry / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D028001:Eleutherococcus / Q000737:chemistry*; D007155:Immunologic Factors / Q000737:chemistry / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009113:Muramidase / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry*; D013154:Spleen / Q000166:cytology
D001704:Biopolymers; D007155:Immunologic Factors; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D009113:Muramidase