title: In-vitro and in-vivo antitumour activity of physalins B and D from Physalis angulata.
abstract: We have evaluated the in-vitro and in-vivo antitumour activity of physalin B and physalin D isolated from the aerial parts of Physalis angulata. In-vitro, both compounds displayed considerable cytotoxicity against several cancer cell lines, showing IC50 values in the range of 0.58 to 15.18 microg mL(-1) for physalin B, and 0.28 to 2.43 microg mL(-1) for physalin D. The antitumour activity of both compounds was confirmed in-vivo using mice bearing sarcoma 180 tumour cells. The in-vivo antitumour activity was related to the inhibition of tumour proliferation, as observed by the reduction of Ki67 staining in tumours of treated animals. Histopathological examination of the kidney and liver showed that both organs were affected by physalin treatment, but in a reversible manner. These compounds were probably responsible for the previously described antitumour activity of ethanol extracts of P. angulata, and their identification and characterization presented here could explain the ethnopharmacological use of this species in the treatment of cancer.
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CP 3157, Ceará 60430-270, Brazil.
authors.forename: Hemerson Iury Ferreira
authors.initials: HI
authors.lastname: Magalhães
authors.forename: Maria Leopoldina
authors.initials: ML
authors.lastname: Veras
authors.forename: Márcia Rocha
authors.initials: MR
authors.lastname: Torres
authors.forename: Ana Paula Negreiros Nunes
authors.initials: AP
authors.lastname: Alves
authors.forename: Otília Deusdênia Loiola
authors.initials: OD
authors.lastname: Pessoa
authors.forename: Edilberto Rocha
authors.initials: ER
authors.lastname: Silveira
authors.forename: Letícia Veras
authors.initials: LV
authors.lastname: Costa-Lotufo
authors.forename: Manoel Odorico
authors.initials: MO
authors.lastname: de Moraes
authors.forename: Cláudia
authors.initials: C
authors.lastname: Pessoa
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D032321:Physalis / Q000737:chemistry; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000473:pathology; D012632:Secosteroids; D013256:Steroids / Q000494:pharmacology*; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D007783:Lactones; D012632:Secosteroids; D013256:Steroids; C441429:physalin D; C033270:physalin B