title: Mechanisms of neurodegeneration in neuronal ceroid-lipofuscinoses.
abstract: Neuronal ceroid-lipofuscinoses (NCL) are a group of neurodegenerative diseases and autosomal recessive lysosomal storage disorders. We examined the involvement of cell death, oxidative stress, and glutamate excitotoxicity using immunohistochemistry against Bcl-2, Bcl-x, oxidative products to proteins, lipids and DNA, calcium-binding proteins (calbindin-D28K, parvalbumin, calretinin), and glial glutamate transporters (excitatory amino acid transporters 1 and 2), in addition to terminal deoxynucleotidyl transferase-mediated dUTP-nick end labeling (TUNEL) in the brains from three cases of late infantile form of NCL (LINCL) and one case of juvenile form of NCL (JNCL) to investigate the neurodegenerative mechanisms. In the cerebral and cerebellar cortex, all of three LINCL cases demonstrated neurons with TUNEL-immunoreactive nuclei, whereas the JNCL case did not show TUNEL-immunoreactive nuclei. The coexistence of the nuclear TUNEL-immunoreactivity nuclei and cytoplasmic deposition of 4-hydroxy-2-nonenal-modified protein in the frontal cortex and hypoglossal nucleus may suggest a possible interrelationship between DNA fragmentation and lipid oxidation in LINCL. Additionally, glycoxidation of protein and oxidative stress to DNA seemed to be involved in the cerebellar and cerebral degeneration, respectively. Interneurons immunoreactive for calbindin-D28K and parvalbumin were severely reduced in the cerebral cortex, whereas those for calretinin were comparatively well preserved in LINCL, indicating the possibility of altered GABAergic system. The disturbance of expression of glial glutamate transporters seemed to be heterogeneous and mild. These findings suggest the possibility of new treatments for neurodegeneration in LINCL using antioxidative agents and/or GABAergic medications.
authors.affiliation: Department of Pediatrics, Metropolitan Fuchu Medical Center for SMID, 2-9-2 Musashi-dai, Fuchu-shi, 183-0042, Tokyo, Japan. Yasuo_Hachiya@member.metro.tokyo.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Hachiya
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hayashi
authors.forename: Satoko
authors.initials: S
authors.lastname: Kumada
authors.forename: Akira
authors.initials: A
authors.lastname: Uchiyama
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Kurata
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002648:Child; D027341:Excitatory Amino Acid Transporter 1 / Q000378:metabolism; D027342:Excitatory Amino Acid Transporter 2; D005260:Female; D050491:Glutamate Plasma Membrane Transport Proteins / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D008297:Male; D009410:Nerve Degeneration / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D009457:Neuroglia / Q000378:metabolism; D009472:Neuronal Ceroid-Lipofuscinoses / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D009498:Neurotoxins / Q000378:metabolism; D018384:Oxidative Stress
chemical_list: D002135:Calcium-Binding Proteins; D027341:Excitatory Amino Acid Transporter 1; D027342:Excitatory Amino Acid Transporter 2; D050491:Glutamate Plasma Membrane Transport Proteins; D009498:Neurotoxins; C493397:SLC1A2 protein, human; C493394:SLC1A3 protein, human; D018698:Glutamic Acid