title: Anti-inflammatory action of dietary fish oil and calorie restriction.
abstract: Inflammation, inflammatory mediators, cyclooxygenase (COX)-2, and inducible nitric oxide (iNOS) are all influenced by age-related oxidative status. To investigate the effect of dietary fish oil (FO) and calorie restriction (CR) on oxidative stress-related inflammatory status with age, (NZB/NZW) F1 (B/W) mice were fed for 4 and 9 months either ad libitum or calorie-restricted (60% of ad libitum intake) diets containing 5% corn oil or 5% FO. We measured several key oxidative and inflammatory markers: TBARS, xanthine oxidase (XOD)-derived superoxide generation, and PGE2 and LTB4 production. Expressions of renal COX-1, COX-2, and iNOS mRNA were analyzed by RT-PCR; additionally, COX-2 protein was estimated by Western-blot method. Results show that FO intake and CR individually and together suppressed age-related increases in lipid peroxidation and superoxide generation. The inhibitory effects of dietary FO and CR were also found for iNOS expression, COX-2 expression, which subsequently led to the suppression of PGE2 and LTB4. We conclude that the beneficial effects of FO feeding and CR are synergistic in ameliorating the age-related nephritis of B/W mice by suppressing COX-2 and iNOS, reactive species generation, and pro-inflammatory mediators.
authors.affiliation: Department of Cosmetology, Pusan Women's College, Pusanjin-Gu, Pusan, Korea.
authors.forename: You Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Hyon Jeen
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: No
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Chung
authors.forename: Gabriel
authors.initials: G
authors.lastname: Fernandes
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000893:Anti-Inflammatory Agents; D015153:Blotting, Western; D031204:Caloric Restriction; D051545:Cyclooxygenase 1 / Q000096:biosynthesis; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D004032:Diet; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D005395:Fish Oils / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism; D007975:Leukotriene B4 / Q000096:biosynthesis; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D005395:Fish Oils; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D007975:Leukotriene B4; D052247:Nitric Oxide Synthase Type II; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D014969:Xanthine Oxidase; D015232:Dinoprostone