A molecular genetic approach for species identification of mammals and sex determination of birds in a forensic case of poaching from South Korea.
DNA-based analysis was performed using partial mitochondrial cytochrome b genes of five mammalian specimens and Chromo-Helicase-DNA-binding (CHD) genes of five pheasants to determine whether specimens were from illegally hunted animals. Mammalian specimens were identified as being those of horse, roe deer, and cow through gene amplification using cytb981f and cytb981r primer set and sequencing. CHD genes were revealed to be those of three male and two female pheasants through polymerase chain reaction amplification. Because hunting of roe deers and female pheasants is prohibited in Korea, these results provided forensic evidences of illegal wild animal hunting.
An
Junghwa
J
Conservation Genome Resource Bank for Korean Wildlife (CGRB), College of Veterinary Medicine, School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea.
Lee
Mu-Yeong
MY
Min
Mi-Sook
MS
Lee
Mun-Han
MH
Lee
Hang
H
D000818:Animals; D001717:Birds / Q000235:genetics*; D002417:Cattle / Q000235:genetics; D003247:Conservation of Natural Resources / Q000331:legislation & jurisprudence*; D045303:Cytochromes b / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D003670:Deer / Q000235:genetics; D005260:Female; D053803:Forensic Genetics; D006736:Horses / Q000235:genetics; D007723:Korea; D008297:Male; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019849:Sex Determination Processes; D013045:Species Specificity
D017931:DNA Primers; D004272:DNA, Mitochondrial; D045303:Cytochromes b