title: Sequence-based typing reveals a novel DLA-88 allele, DLA-88*04501, in a beagle family.
abstract: The dog is an important animal model for solid organ as well as stem cell allo transplantation. Methods such as cellular and serological typing and more recently sequence-based typing (SBT) have been used to discriminate tissue antigen disparity of donor and recipient. We applied SBT for the canine class I (DLA-88) and class II (DLA-DRB1) genes in beagle families prior stem cell transplantation. A novel DLA-88 (DLA-88*04501) allele in combination with a DLA-DRB1*01901 allele was found. Sequence comparison of exons 2 and 3 of the novel allele revealed most sequence identity to the DLA-88*01301 allele (96.15% identity at the nucleotide and 90.65% identity at the protein level).
authors.affiliation: Institute for Immunology, University Hospital of Essen, Germany. cornelia.hardt@medizin.uni-essen.de
authors.forename: C
authors.initials: C
authors.lastname: Hardt
authors.forename: S
authors.initials: S
authors.lastname: Ferencik
authors.forename: R
authors.initials: R
authors.lastname: Tak
authors.forename: P M
authors.initials: PM
authors.lastname: Hoogerbrugge
authors.forename: V
authors.initials: V
authors.lastname: Wagner
authors.forename: H
authors.initials: H
authors.lastname: Grosse-Wilde
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004285:Dogs / Q000235:genetics* / Q000276:immunology; D014644:Genetic Variation; D006239:Haplotypes; D006649:Histocompatibility Antigens / Q000235:genetics*; D008285:Major Histocompatibility Complex / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D035781:Siblings
chemical_list: D006649:Histocompatibility Antigens