[Antiapoptotic functions of the retrovirally transferred API2-MALT1 gene].
t(11 ; 18)(q21 ; q21) is a specific chromosomal aberration in mucosa-associated lymphoid tissue (MALT) lymphoma, and produces chimeric transcript Apoptosis Inhibitor 2 (API2)-MALT1. Although it is known that API2 has an antiapoptotic effect, it is still unclear whether this also applies to API2-MALT1. To investigate its effects against various apoptotic stimuli, API2-MALT1 was expressed by means of retroviral infection on the epithelial cell line HeLa and the murine Pro-B cell line Ba/F3. On both these cell lines, API2-MALT1 was found to cause a significant reduction in UV-induced apoptosis. The apoptosis induced by doxorubicin was also inhibited by API2-MALT1, but not that induced by IL-3 withdrawal from Ba/F3. These findings suggest that API2-MALT1 has an antiapoptotic effect on both epithelial and lymphoid cells and that this effect depends on the apoptotic stimulus.
Mayama
Ko
K
Division of Molecular Medicine, Aichi Cancer Center Research Institute.
Tsuzuki
Shinobu
S
Seto
Masao
M
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11; D002887:Chromosomes, Human, Pair 18; D004317:Doxorubicin / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics*; D051379:Mice; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000502:physiology*; D012190:Retroviridae / Q000235:genetics*; D014178:Translocation, Genetic
C415943:API2-MALT1 fusion protein, human; D015514:Oncogene Proteins, Fusion; D004317:Doxorubicin