title: Detection and identification of intestinal pathogenic bacteria by hybridization to oligonucleotide microarrays.
abstract: OBJECTIVE
  To detect the common intestinal pathogenic bacteria quickly and accurately.
  
  
  METHODS
  A rapid (<3 h) experimental procedure was set up based upon the gene chip technology. Target genes were amplified and hybridized by oligonucleotide microarrays.
  
  
  RESULTS
  One hundred and seventy strains of bacteria in pure culture belonging to 11 genera were successfully discriminated under comparatively same conditions, and a series of specific hybridization maps corresponding to each kind of bacteria were obtained. When this method was applied to 26 divided cultures, 25 (96.2%) were identified.
  
  
  CONCLUSIONS
  Salmonella sp., Escherichia coli, Shigella sp., Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, Proteus sp., Bacillus cereus, Vibrio cholerae, Enterococcus faecalis, Yersinia enterocolitica, and Campylobacter jejuni can be detected and identified by our microarrays. The accuracy, range, and discrimination power of this assay can be continually improved by adding further oligonucleotides to the arrays without any significant increase of complexity or cost.
authors.affiliation: Tianjin Institute of Hygiene and Environmental Medicine 1, Da Li Road, Tianjin 300050, China. jinlianqun@sina.com
authors.forename: Lian-Qun
authors.initials: LQ
authors.lastname: Jin
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Li
authors.forename: Sheng-Qi
authors.initials: SQ
authors.lastname: Wang
authors.forename: Fu-Huan
authors.initials: FH
authors.lastname: Chao
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Zheng-Quan
authors.initials: ZQ
authors.lastname: Yuan
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S