title: SELDI-TOF MS of quadruplicate urine and serum samples to evaluate changes related to storage conditions.
abstract: Proteomic profiling with SELDI-TOF MS has facilitated the discovery of disease-specific protein profiles. However, multicenter studies are often hindered by the logistics required for prompt deep-freezing of samples in liquid nitrogen or dry ice within the clinic setting prior to shipping. We report high concordance between MS profiles within sets of quadruplicate split urine and serum samples deep-frozen at 0, 2, 6, and 24 h after sample collection. Gage R&R results confirm that deep-freezing times are not a statistically significant source of SELDI-TOF MS variability for either blood or urine.
authors.affiliation: Division of Nephrology, Children's Hospital Boston, Boston, MA, USA.
authors.forename: Avram Z
authors.initials: AZ
authors.lastname: Traum
authors.forename: Meghan P
authors.initials: MP
authors.lastname: Wells
authors.forename: Manuel
authors.initials: M
authors.lastname: Aivado
authors.forename: Towia A
authors.initials: TA
authors.lastname: Libermann
authors.forename: Marco F
authors.initials: MF
authors.lastname: Ramoni
authors.forename: Asher D
authors.initials: AD
authors.lastname: Schachter
mesh_terms: D000418:Albumins / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis*; D015925:Cryopreservation / Q000379:methods; D015337:Multicenter Studies as Topic; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors; D014556:Urine / Q000737:chemistry*
chemical_list: D000418:Albumins; D001798:Blood Proteins