Intermittent administration of morphine alters protein expression in rat nucleus accumbens.
Repeated exposure to drugs of abuse causes time-dependent neuroadaptive changes in the mesocorticolimbic system of the brain that are considered to underlie the expression of major behavioral characteristics of drug addiction. We used a 2-D gel-based proteomics approach to examine morphine-induced temporal changes in protein expression and/or PTM in the nucleus accumbens (NAc) of morphine-sensitized rats. Rats were pretreated with saline [1 mL/kg subcutaneously (s.c.)] or morphine (10 mg/kg, s.c.) once daily for 14 days and the animals were decapitated 1 day later. The NAc was extracted and proteins resolved by 2-DE. Several protein functional groups were found to be regulated in the morphine-treated group, representing cytoskeletal proteins, proteins involved in neurotransmission, enzymes involved in energy metabolism and protein degradation, and a protein that regulates translation.
Li
Ka Wan
KW
Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit, Amsterdam, The Netherlands. ka.wan.li@falw.vu.nl
Jimenez
Connie R
CR
van der Schors
Roel C
RC
Hornshaw
Martin P
MP
Schoffelmeer
Anton N M
AN
Smit
August B
AB
D000818:Animals; D004334:Drug Administration Schedule; D015180:Electrophoresis, Gel, Two-Dimensional; D007279:Injections, Subcutaneous; D007526:Isoelectric Point; D008297:Male; D013058:Mass Spectrometry; D008970:Molecular Weight; D009020:Morphine / Q000008:administration & dosage*; D009294:Narcotics / Q000008:administration & dosage*; D009714:Nucleus Accumbens / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D010449:Peptide Mapping; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009294:Narcotics; D011506:Proteins; D009020:Morphine; D010447:Peptide Hydrolases