NMR-based metabonomic study on the subacute toxicity of aristolochic acid in rats.
The subacute toxicity of aristolochic acid (AA) was investigated by (1)H NMR spectroscopic and pattern recognition (PR)-based metabonomic methods. Model toxins were used to enable comparisons of the urinary profiles from rats treated with known toxicants and AA at various time intervals. Urinary (1)H NMR spectra were data-processed and analyzed by pattern recognition method. The result of visual comparison of the spectra showed that AA caused a renal proximal tubular and papillary lesion and a slight hepatic impair. Pattern recognition analysis indicated that the renal proximal tubule lesion was the main damage induced by AA, and the renal toxicity induced by AA was a progressive course with the accumulation of dosage by monitoring the toxicological processes from onset, development and part-recovery. These results were also supported by the conventional clinical biochemical parameters.
Zhang
Xiaoyu
X
Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Changchun 130022, PR China.
Wu
Huifeng
H
Liao
Peiqiu
P
Li
Xiaojing
X
Ni
Jiazuan
J
Pei
Fengkui
F
D000818:Animals; D034341:Aristolochic Acids / Q000737:chemistry* / Q000633:toxicity*; D001774:Blood Chemical Analysis; D001835:Body Weight / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010363:Pattern Recognition, Automated; D051381:Rats; D017208:Rats, Wistar; D014118:Toxins, Biological / Q000652:urine; D015430:Weight Gain / Q000187:drug effects
D034341:Aristolochic Acids; D014118:Toxins, Biological; C000228:aristolochic acid I