title: [Experimental study of the effect of Bushen Huoxue recipe on biological characters of osteoblasts in vitro].
abstract: OBJECTIVE
  To observe the effect of Bushen Huoxue Recipe on cell proliferation, differentiation, maturation and mineralization in rats cultured osteoblasts.
  
  
  METHODS
  Osteoblasts from craniums of newly born SD rats were cultured in vitro. MTT, PNPP and ARS were used to observe the proliferation, activity of ALP and the number of mineral node of cultured osteoblasts in vitro.
  
  
  RESULTS
  It was found that Bushen Huoxue Recipe had the effect on stimulating cell proliferation, activity of ALP and the number of mineral node of cultured osteoblasts (P < 0.01 or P < 0.05).
  
  
  CONCLUSIONS
  Bushen Huoxue Recipe can promote proliferation, differentiation, maturation and mineralization of the osteoblasts cultured in vitro.
authors.affiliation: Department of Orthopedics, Second Hospital of Xi' an Jiaotong University, Xi' an 710004.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Zang
authors.forename: Junchang
authors.initials: J
authors.lastname: Chen
authors.forename: Yiheng
authors.initials: Y
authors.lastname: Liu
authors.forename: Runxia
authors.initials: R
authors.lastname: Liu
authors.forename: Kunzheng
authors.initials: K
authors.lastname: Wang
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000166:cytology
chemical_list: D004365:Drugs, Chinese Herbal; D000469:Alkaline Phosphatase