title: Anaerobic bio-hydrogen production using pre-heated river sediments as seed sludge.
abstract: Anaerobic bio-hydrogen production is the focus in the field of bio-energy resources. In this paper, a series of batch experiments were conducted to investigate the effects of several factors on anaerobic bio-hydrogen producing process carried out by pre-heated river sediments. The results showed that several factors such as substrate and its concentration, temperature and the initial pH value could affect the anaerobic bio-hydrogen production in different levels. At 35 degrees C and the initial pH of 6.5, using glucose of 20,000 mg COD/L as substrate, the highest hydrogen production of 323.8 ml-H2/g TVS in a 100 ml batch reactor was reached, the specific hydrogen production rate was 37.7 ml-H2/g TVS h, and the hydrogen content was 51.2%. Thereafter using the same pre-heated river sediments as seed sludge, continuous anaerobic bio-hydrogen production was achieved successfully in a lab-scale CSTR with gas-separator. At the organic loading rate of 36 kg COD/m3 d, the highest hydrogen production was 6.3-6.7 l-H2/l-reactor d, the specific hydrogen production was 1.3-1.4 mol-H2/mol-glucose, and the hydrogen content in the gas was 52.3%. The effluent of the bio-reactor contained some small molecular organics, mainly including ethanol, acetate, butyrate and their molar proportion is 1:1:0.6.
authors.affiliation: Department of Environmental Science and Engineering, Tsinghua University, Beijing, 100084, PR China.
authors.forename: J
authors.initials: J
authors.lastname: Zuo
authors.forename: Y
authors.initials: Y
authors.lastname: Zuo
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Chen
mesh_terms: D000085:Acetates / Q000378:metabolism; D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002087:Butyrates / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D045483:Rivers; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D013696:Temperature; D013997:Time Factors; D014865:Waste Disposal, Fluid / Q000379:methods*; D014872:Water Movements
chemical_list: D000085:Acetates; D002087:Butyrates; D012722:Sewage; D000431:Ethanol; D006859:Hydrogen; D005947:Glucose; D010100:Oxygen