Pediococcus stilesii sp. nov., isolated from maize grains.
A Gram-positive, coccus-shaped, lactic acid bacterium, strain LMG 23082T, was isolated from steeped maize grains. The organism is homofermentative and produces D- and L-lactic acid from glucose. 16S rRNA gene sequence analysis revealed that the organism belongs to the genus Pediococcus, with Pediococcus pentosaceus and Pediococcus acidilactici as nearest neighbours. Genotypic fingerprinting, whole-cell protein electrophoresis, DNA-DNA hybridizations and physiological and biochemical tests allowed differentiation of strain LMG 23082T from other established Pediococcus species. A remarkable feature was that, unlike other pediococci, this bacterium was capable of growth at pH 9.0. The strain studied represents a novel species for which the name Pediococcus stilesii sp. nov. is proposed with the type strain LMG 23082T (=BFE 1652T=FAIR-E 180T=CCUG 51290T), the only currently known isolate of the species.
Franz
Charles M A P
CMAP
Federal Research Centre for Nutrition and Food, Institute for Hygiene and Toxicology, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany.
Vancanneyt
Marc
M
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
Vandemeulebroecke
Katrien
K
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
De Wachter
Marjan
M
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
Cleenwerck
Ilse
I
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
Hoste
Bart
B
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
Schillinger
Ulrich
U
Federal Research Centre for Nutrition and Food, Institute for Hygiene and Toxicology, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany.
Holzapfel
Wilhelm H
WH
Federal Research Centre for Nutrition and Food, Institute for Hygiene and Toxicology, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany.
Swings
Jean
J
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010376:Pediococcus / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D003313:Zea mays / Q000382:microbiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S