title: Developmental alterations in thymocyte sensitivity are actively regulated by MHC class II expression in the thymic medulla.
abstract: Developing thymocytes are positively selected if they respond to self-MHC-peptide complexes, yet mature T cells are not activated by those same self-complexes. To avoid autoimmunity, positive selection must be followed by a period of maturation when the cellular response to TCR signals is altered. The mechanisms that mediate this postselection developmental tuning remain largely unknown. Specifically, it is unknown whether developmental tuning is a preprogrammed outcome of positive selection or if it is sensitive to ongoing interactions between the thymocyte and the thymic stroma. We probed the requirement for MHC class II-TCR interactions in postselection maturation by studying single positive (SP) CD4 thymocytes from K14/A(beta)(b) mice, in which CD4 T cells cannot interact with MHC class II in the thymic medulla. We report here that SP CD4 thymocytes must receive MHC class II signals to avoid hyperactive responses to TCR signals. This hyperactivity correlates with decreased expression of CD5; however, developmental tuning can occur independently of CD5, correlating instead with differences in the distribution of Lck. Thus, the maturation of postselection SP CD4 thymocytes is an active process mediated by ongoing interactions between the T cell and MHC class II molecules. This represents a novel mechanism by which the thymic medulla prevents autoreactivity.
authors.affiliation: Department of Medicine, University of Pennsylvania, Philadephia, 19104, USA.
authors.forename: Steven C
authors.initials: SC
authors.lastname: Eck
authors.forename: Peimin
authors.initials: P
authors.lastname: Zhu
authors.forename: Marion
authors.initials: M
authors.lastname: Pepper
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Bensinger
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Freedman
authors.forename: Terri M
authors.initials: TM
authors.lastname: Laufer
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism; D018956:CD5 Antigens / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000276:immunology / Q000378:metabolism*; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*
chemical_list: D015704:CD4 Antigens; D018956:CD5 Antigens; D000949:Histocompatibility Antigens Class II; D011948:Receptors, Antigen, T-Cell; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)