Anthocyanins from soybean seed coat inhibit the expression of TNF-alpha-induced genes associated with ischemia/reperfusion in endothelial cell by NF-kappaB-dependent pathway and reduce rat myocardial damages incurred by ischemia and reperfusion in vivo.
We examined the inhibition of the expression of some inflammatory genes associated with ischemia-reperfusion (I/R) injury by anthocyanins isolated from black soybean seed coat in tumor necrosis factor-alpha (TNF-alpha)-treated bovine aortic endothelial cells. In addition, its potential use on I/R-injury was investigated using rats subjected to 30-min occlusion of left descending coronary artery followed by 24-h reperfusion. Western blot analysis and luciferase activity assay showed that anthocyanins inhibited TNF-alpha-induced vascular cell adhesion molecule-1, intracellular adhesion molecule-1, and cyclooxygenase-2 levels, which is through NF-kappaB-dependent pathway. Further, anthocyanins protected myocardiac injury from I/R in rats. It is suggested that anthocyanins from black soybean seed coat can be used as a useful drug to modulate cardiovascular disorder.
Kim
Hye Jung
HJ
Department of Pharmacology, School of Medicine and Institute of Health Sciences, Gyeongsang National University, Republic of Korea.
Tsoy
Irina
I
Park
Jung Min
JM
Chung
Jong Il
JI
Shin
Sung Chul
SC
Chang
Ki Churl
KC
D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology*; D002417:Cattle; D051546:Cyclooxygenase 2 / Q000235:genetics; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D015428:Myocardial Reperfusion Injury / Q000235:genetics / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism; D051381:Rats; D015427:Reperfusion Injury / Q000235:genetics*; D012639:Seeds; D013025:Glycine max; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
D000872:Anthocyanins; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D051546:Cyclooxygenase 2