Comparative genomic organization of the human and bovine PRNP locus.
We sequenced a 208-kb BAC clone spanning the bovine prion protein (PRNP) locus, and compared the genomic structure with that of human. As a result, we determined the precise breakpoint between the two syntenic genomes, located on the 5' UTR of the PRNP gene, and discovered two highly repetitive sequences near the breakpoint. Further analysis demonstrated that the genomic structure of three genes, PRNP, PRND, and RASSF2, within the syntenic region of the bovine genome is highly conserved in order and orientation. The PRNT locus was not found in bovine but is conserved in several primates, including human. Moreover, we confirmed that the bovine RASSF2 is composed of 10 exons, as is the human gene, showing some difference from a previous report. Our findings may provide useful clues for understanding the evolutional process in the PRNP locus and also the mechanism that allows TSE from cattle to infect humans.
Choi
Sang-Haeng
SH
Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, 52, Oun-dong, Yusong-gu, Daejeon 305-333, Korea.
Kim
Il-Chul
IC
Kim
Dae-Soo
DS
Kim
Dae-Won
DW
Chae
Sung-Hwa
SH
Choi
Han-Ho
HH
Choi
Inho
I
Yeo
Jung-Sou
JS
Song
Mi-Na
MN
Park
Hong-Seog
HS
D000682:Amyloid / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D022202:Chromosomes, Artificial, Bacterial; D058851:GPI-Linked Proteins; D006801:Humans; D008252:Macaca fascicularis / Q000235:genetics; D008969:Molecular Sequence Data; D011323:Primates / Q000235:genetics; D000072002:Prion Proteins; D011328:Prions / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics*; D011506:Proteins / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D026801:Synteny; D025521:Tumor Suppressor Proteins
D000682:Amyloid; D058851:GPI-Linked Proteins; C501125:PRND protein, human; C512656:PRNP protein, human; D000072002:Prion Proteins; D011328:Prions; D011498:Protein Precursors; D011506:Proteins; C425300:RASSF2 protein, human; D025521:Tumor Suppressor Proteins