title: Effect of mitomycin C on the secretion of granulocyte macrophages colonies stimulating factor and interleukin-5 in eosinophilic nasal polyps stromal culture.
abstract: The research involving tissue factors, such as granulocyte macrophage colonies stimulating factor (GM-CSF) and interleukin 5 (IL-5), leads to the mechanisms involved in the maintenance of eosinophilia, which is essential for the pathogenesis on eosinophilic nasal polyps. Mitomycin C has been successfully used in otolaryngology.
  
  
  OBJECTIVE
  The objective of this study was to evaluate the effect of mitomycin C in secretion of GM-CSF and IL-5 on eosinophilic nasal polyps.
  
  
  METHODS
  Case-control.
  
  
  METHODS
  This is a comparative and auto-matched experimental study, performed with fragments of polyps which had been obtained from biopsy of patients with eosinophilic nasosinusal polyposis. The fragments of the experimental group were treated with mitomycin C (400 microg/ml) for 5 minutes and then washed in RPMI substrate. At time zero, 12 and 24 hours, the surface material was taken to determination of its GM-CSF levels in 22 patients and of IL-5 levels in 19 patients, by ELISA method.
  
  
  RESULTS
  Reduction in GM-CSF expression on the experimental group at time 24 h (p< or = 0.05). The treated group presented significant GM-CSF expression between zero time and 12 h time (p= 0.013) showing the culture viability such as in the non-treated group. Tendency to decreasing IL-5 levels on the treated groups at 24 hours.
  
  
  CONCLUSIONS
  This study showed that mitomycin C was efficient in inhibiting GM-CSF synthesis with reduction of IL-5 secretion, but this fact needs complementary studies.
authors.affiliation: Department of Ophthalmology, Otorhinolaryngology and Speech and Hearing Therapy, Federal University of Minas Gerais. paulocrosara@hc.ufmg.br
authors.forename: Paulo Fernando Tormin Borges
authors.initials: PF
authors.lastname: Crosara
authors.forename: Anilton César
authors.initials: AC
authors.lastname: Vasconcelos
authors.forename: Roberto Eustáquio Santos
authors.initials: RE
authors.lastname: Guimarães
authors.forename: Helena Maria Gonçalves
authors.initials: HM
authors.lastname: Becker
authors.forename: Celso Gonçalves
authors.initials: CG
authors.lastname: Becker
authors.forename: Sandra Letícia Reis
authors.initials: SL
authors.lastname: Crosara
authors.forename: Evaldo
authors.initials: E
authors.lastname: Nascimento
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D016022:Case-Control Studies; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D015848:Interleukin-5 / Q000096:biosynthesis*; D008875:Middle Aged; D016685:Mitomycin / Q000494:pharmacology*; D009298:Nasal Polyps / Q000378:metabolism* / Q000473:pathology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D015848:Interleukin-5; D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor