Citrate-release-mediated aluminum resistance is coupled to the inducible expression of mitochondrial citrate synthase gene in Paraserianthes falcataria.
Aluminum (Al) resistance in some leguminous plants is achieved by enhanced citrate release from roots. Enhancement requires several hours for complete activation and is postulated to involve Al-responsive genes or components. We examined the mechanism of Al-induced citrate release by studying the relationship between citrate release and expression of the mitochondrial citrate synthase (mCS) gene in three leguminous trees. Root elongation in Leucaena leucocephala (Lam.) de Wit was arrested within 24 h by 30 microM Al, whereas root elongation in Paraserianthes falcataria (L.) Neilson and Acacia mangium Willd. was inhibited < 50% by a 48-h treatment with 100 microM Al in calcium chloride solution. Roots of P. falcataria and A. mangium maintained enhanced release and accumulation of citrate for at least 28 days in response to Al treatment. Aluminum increased the accumulation of mCS transcripts in P. falcataria roots, but not in L. leucocephala roots, and thus up-regulation decreased following removal of Al. Lanthanum did not alter the expression level of mCS. Aluminum increased mCS activity concomitantly with enhanced mCS gene expression in P. falcataria, whereas it did not affect mCS activity in L. leucocephala. Aluminum content in root apices of P. falcataria was increased by cycloheximide, supporting the idea that de novo synthesis of proteins is a prerequisite for Al resistance. Our findings suggest that Al-inducible expression of mCS coupled with enhanced citrate release mediates Al resistance in P. falcataria.
Osawa
Hiroki
H
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. osawa@fr.a.u-tokyo.ac.jp
Kojima
Katsumi
K
D000535:Aluminum / Q000378:metabolism / Q000494:pharmacology*; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002950:Citrate (si)-Synthase / Q000235:genetics* / Q000378:metabolism; D002951:Citrates / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D004351:Drug Resistance; D007887:Fabaceae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid
D002951:Citrates; D024101:Mitochondrial Proteins; D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide; D000535:Aluminum; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+); D002950:Citrate (si)-Synthase