title: Determination of the degree of acetylation of chitosan by UV spectrophotometry using dual standards.
abstract: Determination of the degree of acetylation of chitosan by UV spectrophotometry using dual standards is investigated. The UV absorbance of a pure chitosan solution is contributed additively by the N-acetylglucosamine and glucosamine residues; the absorbance divided by the total molar concentration of the residues (A/c(t)) is linearly related to the degree of acetylation (DA). Using acetyl glucosamine and glucosamine hydrochloride as standards in 0.1M hydrochloric acid solution, the equation obtained by linear regression is A/c(t)=3.3615 DA+0.0218, R(2)=0.9958. The DA of the analytical sample (m milligram of chitosan in V liters solution) can be calculated by.
authors.affiliation: Research Center for Sugar Engineering and Technology, Guangxi University, Nanning, 530004, Peoples Republic of China. dsliu@gxu.edu.cn
authors.forename: Dasheng
authors.initials: D
authors.lastname: Liu
authors.forename: Yuanan
authors.initials: Y
authors.lastname: Wei
authors.forename: Pingjia
authors.initials: P
authors.lastname: Yao
authors.forename: Linbin
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000107:Acetylation; D000818:Animals; D003386:Brachyura; D048271:Chitosan / Q000737:chemistry*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D048271:Chitosan