title: Characterization of two D-beta-hydroxybutyrate dehydrogenase populations in heavy and light mitochondria from jerboa (Jaculus orientalis) liver.
abstract: Mitochondrial membrane-bound and phospholipid-dependent D-beta-hydroxybutyrate dehydrogenase (BDH) (EC 1.1.1.30), a ketone body converting enzyme in mitochondria, has been studied in two populations of mitochondria (heavy and light) of jerboa (Jaculus orientalis) liver. The results reveal significant differences between the BDH of the two mitochondrial populations in terms of protein expression, kinetic parameters and physico-chemical properties. These results suggest that the beta-hydroxybutyrate dehydrogenases from heavy and light mitochondria are isoform variants. These differences in BDH distribution could be the consequence of cell changes in the lipid composition of the inner mitochondrial membrane of heavy and light mitochondria. These changes could modify both BDH insertion and BDH lipid-dependent catalytic properties.
authors.affiliation: Laboratoire de Biochimie, Faculté des Sciences, Université Hassan II-Aïn Chock, km 8 route d'El Jadida BP. 5366, Casablanca, Morocco. drissmountassif@yahoo.fr
authors.forename: Driss
authors.initials: D
authors.lastname: Mountassif
authors.forename: Mostafa
authors.initials: M
authors.lastname: Kabine
authors.forename: Norbert
authors.initials: N
authors.lastname: Latruffe
authors.forename: M'hammed Saïd
authors.initials: MS
authors.lastname: El Kebbaj
mesh_terms: D000818:Animals; D006884:Hydroxybutyrate Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification; D007700:Kinetics; D008930:Mitochondria, Liver / Q000201:enzymology*; D012377:Rodentia / Q000378:metabolism*
chemical_list: D006884:Hydroxybutyrate Dehydrogenase