title: Etiologic diagnosis of adult bacterial pneumonia by culture and PCR applied to respiratory tract samples.
abstract: Respiratory culture and multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, and Chlamydophila pneumoniae were applied to sputum, nasopharyngeal swabs, and nasopharyngeal aspirates from 235 adult patients with community-acquired pneumonia and 113 controls. Both culture and multiplex PCR performed well with the different samples and appear to be useful as diagnostic tools.
authors.affiliation: Department of Infectious Diseases, Orebro University Hospital, SE-70185 Orebro, Sweden. kristoffer.stralin@orebroll.se
authors.forename: Kristoffer
authors.initials: K
authors.lastname: Strålin
authors.forename: Eva
authors.initials: E
authors.lastname: Törnqvist
authors.forename: Margit Staum
authors.initials: MS
authors.lastname: Kaltoft
authors.forename: Per
authors.initials: P
authors.lastname: Olcén
authors.forename: Hans
authors.initials: H
authors.lastname: Holmberg
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques; D016993:Chlamydophila pneumoniae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017714:Community-Acquired Infections / Q000175:diagnosis / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D009177:Mycoplasma pneumoniae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D018410:Pneumonia, Bacterial / Q000175:diagnosis* / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012137:Respiratory System / Q000382:microbiology; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial