title: Evaluation of the new GenoType Mycobacterium assay for identification of mycobacterial species.
abstract: The new commercial assay GenoType Mycobacterium, intended for the identification of mycobacteria, was evaluated with a panel of 197 strains belonging to 86 different taxa. The system, which relies on solid-phase reverse hybridization, is composed of two different kits: GenoType CM for the identification of more frequently detected mycobacterial species and GenoType AS for less common species. The sensitivity and specificity were 97.9% and 92.4% for the CM kit and 99.3% and 99.4% for the AS kit. Our results show that the system may represent a useful tool to substantially enlarge the number of mycobacteria that can be reliably identified in clinical laboratories.
authors.affiliation: Centro Regionale di Riferimento per la Diagnostica dei Micobatteri, Piastra dei Servizi, Ospedale di Careggi, viale Morgagni 85, 50134 Firenze, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Russo
authors.forename: Enrico
authors.initials: E
authors.lastname: Tortoli
authors.forename: Donato
authors.initials: D
authors.lastname: Menichella
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D011933:Reagent Kits, Diagnostic