title: Aureobasidium pullulans morphology: two adapted polysaccharide stains.
abstract: Morphological stages of Aureobasidium pullulans were investigated utilizing different media ingredients and were visualized by bright-field microscopy. A polysaccharide stain was developed to stain chlamydospores, cell walls, hyphae, and conidia, since current staining techniques do not reveal subcellular details to identify fungi, especially those that exhibit polysaccharide secretions.
authors.affiliation: Department of Biology, Central Missouri State University, Warrensburg, MO 64093, USA. oller@cmsu1.cmsu.edu
authors.forename: Anna R
authors.initials: AR
authors.lastname: Oller
mesh_terms: D001203:Ascomycota / Q000737:chemistry / Q000166:cytology* / Q000302:isolation & purification; D005936:Glucans; D008853:Microscopy; D009172:Mycology / Q000379:methods; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005936:Glucans; C009109:pullulan