A transformation system for the hypotrichous ciliate Stylonychia mytilus.
We describe a transformation system for the ciliate Stylonychia mytilus. The neomycin resistance gene from Escherichia coli transposon Tn5, which codes for the enzyme phosphotransferase and confers resistance to the antibiotic G 418, was ligated into macronuclear ;gene-size' DNA molecules. Using this recombinant DNA for transformation experiments we show that the gene is replicated and expressed in transformed cells.
Wünning
I U
IU
Institut für Biologie III, Abt. Zellbiologie, Universität Tübingen, Auf der Morgenstelle 28, D-7400 Tübingen, FRG.
Lipps
H J
HJ