Interaction analysis of the AAA ATPase TAA43 by the bacterial two-hybrid system.
The TAA43 ATPase of Thermoplasma acidophilum, an archaeal member of the AAA protein family, is known to have an atypical oligomeric state and a nonspecific association with high-molecular-weight protein complexes. We assessed the in vivo binding pattern of TAA43 using the bacterial two-hybrid system. We found 36 positive isolates interacting with TAA43. Our analysis showed that TAA43 interacts preferentially with nonribosomal proteins containing ribosomal domains and regions involved in RNA metabolism.
Santos
Leticia
L
Division of Molecular Biology, Institute for Scientific and Technological Research of San Luis Potosi, San Luis Potosi, Mexico. lsantos@ipicyt.edu.mx
Zwickl
Peter
P
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D019843:Archaeal Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D013822:Thermoplasma / Q000201:enzymology*; D020798:Two-Hybrid System Techniques
D019843:Archaeal Proteins; D011994:Recombinant Proteins; D000251:Adenosine Triphosphatases