Polarized transport of Frizzled along the planar microtubule arrays in Drosophila wing epithelium.
Cells in a variety of developmental contexts sense extracellular cues that are given locally on their surfaces, and subsequently amplify the initial signal to achieve cell polarization. Drosophila wing cells acquire planar polarity along the proximal-distal (P-D) axis, in which the amplification of the presumptive cue involves assembly of a multiprotein complex that spans distal and proximal boundaries of adjacent cells. Here we pursue the mechanisms that place one of the components, Frizzled (Fz), at the distal side. Intracellular particles of GFP-tagged Fz moved preferentially toward distal boundaries before Fz::GFP and other components were tightly localized at the P/D cortex. Arrays of microtubules (MTs) were approximately oriented along the P-D axis and these MTs contributed to the formation of the cortical complex. Furthermore, there appeared to be a bias in the P-D MTs, with slightly more plus ends oriented distally. The hypothesis of polarized vesicular trafficking of Fz is discussed.
Shimada
Yuko
Y
Laboratory of Cell Recognition and Pattern Formation, Graduate School of Biostudies, South Campus Research Building (Building G), Room 118, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
Yonemura
Shigenobu
S
Ohkura
Hiroyuki
H
Strutt
David
D
Uemura
Tadashi
T
D048868:Adaptor Proteins, Signal Transducing; D022005:Adherens Junctions / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D001693:Biological Transport, Active; D015820:Cadherins / Q000378:metabolism; D016764:Cell Polarity; D000072261:Dishevelled Proteins; D004330:Drosophila / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D051157:Frizzled Receptors; D017344:Genes, Insect; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008854:Microscopy, Electron; D008870:Microtubules / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D010750:Phosphoproteins / Q000378:metabolism; D011679:Pupa / Q000166:cytology / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014921:Wings, Animal / Q000166:cytology / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D015820:Cadherins; D000072261:Dishevelled Proteins; D029721:Drosophila Proteins; D051157:Frizzled Receptors; D008565:Membrane Proteins; D010750:Phosphoproteins; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; C058784:fz protein, Drosophila; C121420:stan protein, Drosophila; D049452:Green Fluorescent Proteins