title: Inflammatory responses to the occupational inhalation of metal fume.
abstract: Occupational exposure to metal fume promotes a reversible increase in the risk of pneumonia, but by mechanisms which are unclear. To investigate, the current authors measured various markers of host defence function in welders and nonwelders. Induced sputum and venous blood samples were collected from 27 welders with regular long-term exposure to ferrous metal fume and 31 unexposed matched controls. In sputum, the present authors measured cell counts, the soluble and cellular iron concentration, and levels of interleukin-8, tumour necrosis factor-alpha, myeloperoxidase, matrix metalloproteinase-9, immunoglobulin (Ig)A, alpha(2)-macroglobulin and unsaturated iron-binding capacity. Blood samples were assayed for evidence of neutrophil activation and pneumococcal IgG antibodies. Welders had significantly higher iron levels and a substantially lower unsaturated iron-binding capacity in their sputum, but, despite a high iron challenge, there was a noteworthy absence of an inflammatory response. Only blood counts of eosinophils and basophils were significantly related to the extent of welding. Weak nonsignificant trends were observed for several other measures, consistent with low-grade priming of neutrophils. In conclusion, these data suggest that chronic exposure to metal fume blunts responsiveness to inhaled particulate matter. However, the mechanism behind the lack of detectable local inflammatory response requires further investigation.
authors.affiliation: Medical Research Council (MRC) Environmental Epidemiology Unit, University of Southampton, UK. ktp@mrc.soton.ac.uk
authors.forename: K T
authors.initials: KT
authors.lastname: Palmer
authors.forename: R M C
authors.initials: RM
authors.lastname: McNeill Love
authors.forename: R
authors.initials: R
authors.lastname: McNeill-Love
authors.forename: J R
authors.initials: JR
authors.lastname: Poole
authors.forename: D
authors.initials: D
authors.lastname: Coggon
authors.forename: A J
authors.initials: AJ
authors.lastname: Frew
authors.forename: C H
authors.initials: CH
authors.lastname: Linaker
authors.forename: J K
authors.initials: JK
authors.lastname: Shute
mesh_terms: D000293:Adolescent; D000328:Adult; D000395:Air Pollutants, Occupational / Q000633:toxicity*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005740:Gases / Q000633:toxicity*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology*; D019570:Inhalation Exposure; D007501:Iron / Q000032:analysis; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D011014:Pneumonia / Q000139:chemically induced / Q000276:immunology; D016012:Poisson Distribution; D011863:Radioimmunoassay; D013183:Sputum / Q000737:chemistry; D014896:Welding
chemical_list: D000395:Air Pollutants, Occupational; D015415:Biomarkers; D005740:Gases; D007501:Iron