Changes in hypothalamic-pituitary-adrenal function, body temperature, body weight and food intake with repeated social stress exposure in rats.
These present studies aimed to compare changes in hypothalamic-pituitary-adrenal (HPA) activity and body temperature in response to acute social defeat, to repeated social stress and to novel restraint after repeated stress, as well as to assess effects on metabolic parameters by measuring body weight gain and food and water intake. We found that social defeat produced a marked increase in both adrenocorticotrophic hormone and corticosterone compared to placement in a novel cage. Similarly, body temperature was also increased during social defeat and during 30 min of recovery from defeat. We then examined the effects of 6 days of repeated social stress and observed minimal HPA responses to repeated social stress compared to control rats. These neuroendocrine responses were contrasted by robust increases in body temperature during stress and during recovery from stress during 6 days of repeated stress. However, in response to novel restraint, repeatedly stressed rats displayed facilitated body temperature responses compared to controls, similar to our previous findings with HPA activity. Food intake was increased during the light period during which defeat took place, but later intake during the dark period was not affected. Repeated stress decreased body weight gain in the dark period but food intake was increased overall during the 6 days of repeated stress in the light period. As a result, repeated stress increased cumulative food intake during the light period in the stressed rats but these relatively small increases in food intake were unable to prevent the diminished total weight gain in repeatedly stressed rats. Overall, the results demonstrate that, although acute social defeat has similar effects on temperature and HPA activity, repeated exposure to social stress has divergent effects on HPA activity compared to body temperature and that dampened weight gain produced by repeated social stress cannot be fully explained by changes in food intake.
Bhatnagar
S
S
Department of Anaesthesiology, Abranson Building Ste. 402, Children's Hospital of Philadelphia, 34th & Civic Center Boulevard, Philadelphia, PA 19104, USA. bhatnagars@email.chop.edu
Vining
C
C
Iyer
V
V
Kinni
V
V
D000222:Adaptation, Physiological; D000324:Adrenocorticotropic Hormone / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001069:Appetite Regulation / Q000502:physiology; D001831:Body Temperature / Q000502:physiology*; D001835:Body Weight / Q000502:physiology*; D003345:Corticosterone / Q000097:blood; D004435:Eating / Q000502:physiology*; D004734:Energy Metabolism / Q000502:physiology; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism*; D008297:Male; D009043:Motor Activity / Q000502:physiology; D017440:Photoperiod; D010913:Pituitary-Adrenal System / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D012931:Social Environment; D013315:Stress, Psychological / Q000378:metabolism*
D000324:Adrenocorticotropic Hormone; D003345:Corticosterone