title: Methylene blue staining method for identification of Opisthorchis viverrini egg.
abstract: Methylene blue staining method was used to distinguish O. viverrini eggs from Haplorchis taichui and Prosthodendrium molenkampi eggs. All eggs were obtained from dissected adult worms, fixed in 10% formalin, and stained with methylene blue prior to light microscopy observation. The distinct musk-melon-like texture of the O. viverini eggshell surface and the thread-like texture of H. taichui eggshell surface were recognized, while P. molenkampi eggs showed a smooth eggshell. We also evaluated the sensitivity and specificity of the method by training investigators to differentiate surface textures. After training, the investigators were randomly tested with 10 slides containing fluke eggs. The sensitivity and specificity were 95% and 95%, respectively.
authors.affiliation: Department of Parasitology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand. tmsps@mahidol.ac.th
authors.forename: Sataporn
authors.initials: S
authors.lastname: Pasuralertsakul
authors.forename: Warunee
authors.initials: W
authors.lastname: Ngrenngarmlert
authors.forename: Sompong
authors.initials: S
authors.lastname: Sripochang
authors.forename: Nattawut
authors.initials: N
authors.lastname: Khantiyanan
authors.forename: Opas
authors.initials: O
authors.lastname: Akkara-ngamsiri
mesh_terms: D000818:Animals; D004396:Coloring Agents; D006801:Humans; D008751:Methylene Blue; D008859:Microscopy, Polarization; D009889:Opisthorchiasis / Q000175:diagnosis*; D009891:Opisthorchis / Q000145:classification / Q000302:isolation & purification*; D010063:Ovum / Q000166:cytology*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013194:Staining and Labeling
chemical_list: D004396:Coloring Agents; D008751:Methylene Blue