Celecoxib inhibits nitric oxide production in chondrocytes of ligament-damaged osteoarthritic rat joints.
The purpose of this study was to demonstrate the direct effects of celecoxib, one of the selective cyclo-oxygenase (COX)-2 inhibitors, on nitric oxide (NO) and prostaglandinE2 (PGE2) synthesis in cultured osteoarthritic chondrocyte comparing with those of indomethacin. Articular chondrocytes were isolated from rat osteoarthritic knee joint with damaged anterior cruciate ligament and also from the sham knee joint. Chondrocytes were preincubated with or without IL-1 alpha, and were exposed to celecoxib, indomethacin (non-selective COX inhibitor), or nothing. The amounts of NO and PGE2 in culture supernatants of chondrocytes were measured by EIA or the Griess reaction. In a series of experiments preincubated with or without IL-1 alpha and exposed to nothing, PGE2 and NO levels were significantly higher in osteoarthritic chondrocytes than in sham chondrocytes. Celecoxib and indomethacin inhibited the increase of PGE2 in osteoarthritic chondrocytes. Celecoxib inhibited and indomethacin did not inhibit the increase of NO levels in osteoarthritic chondrocytes.
Matsuda
Kenta
K
Department of Orthopaedic Surgery, Teikyo University School of Medicine, 2-11-1 Kaga, 173-8605, Tokyo, Japan. kanta_osteolysis_1970_3_14@yahoo.co.jp
Nakamura
Shigeru
S
Matsushita
Takashi
T
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000473:pathology; D000068579:Celecoxib; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D007213:Indomethacin / Q000494:pharmacology; D053582:Interleukin-1alpha / Q000378:metabolism; D007719:Knee Joint / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis*; D010003:Osteoarthritis / Q000378:metabolism* / Q000473:pathology; D011720:Pyrazoles / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013449:Sulfonamides / Q000494:pharmacology*
D016861:Cyclooxygenase Inhibitors; D053582:Interleukin-1alpha; D011720:Pyrazoles; D013449:Sulfonamides; D009569:Nitric Oxide; D000068579:Celecoxib; D015232:Dinoprostone; D007213:Indomethacin