title: Hyper-reactive mononuclear cells and neutrophils in chronic periodontitis.
abstract: OBJECTIVE
  Stimulated mono- and polymorphonuclear cells from patients with periodontitis have shown increased release of interleukin-1beta (IL-1beta) and oxygen radicals, respectively. The aim was to study whether this hyper-reactivity could be found both in mono- and polymorphonuclear cells from the same patient, and whether there was a relation to the gene coding for IL-1beta (IL-1beta(+3953)).
  
  
  METHODS
  Peripheral mononuclear cells from 14 non-smoking and well-treated patients and pair-matched controls were incubated with opsonized Staphylococcus aureus and lipopolysaccharide (LPS). Released IL-1beta and tumour necrosis factor (TNF)-alpha were determined with ELISA. Generation of oxygen radicals from the Fcgamma-receptor-stimulated neutrophils was measured with chemiluminescence and the polymorphism at IL-1beta(+3953) was measured with polymerase chainreaction.
  
  
  RESULTS
  The mononuclear cells from the patients released more IL-1beta after incubation with LPS (p<0.001) and with bacteria (p<0.05). The release of TNF-alpha tended to be higher in the patient group. The peripheral neutrophils from the patients generated more oxygen radicals (p<0.06). We found no differences between the study groups regarding the IL-1beta(+3953) polymorphism.
  
  
  CONCLUSIONS
  The similarity in systemic inflammation between patients and controls suggests that the increased release/generation of IL-1beta and oxygen radicals from peripheral leukocytes in periodontitis patients is of a constitutional nature and of pathogenic relevance.
authors.affiliation: Department of Odontology, Division of Periodontology, Karolinska Institutet, Stockholm, Sweden. Anders.Gustafsson@ki.se
authors.forename: A
authors.initials: A
authors.lastname: Gustafsson
authors.forename: H
authors.initials: H
authors.lastname: Ito
authors.forename: B
authors.initials: B
authors.lastname: Asman
authors.forename: K
authors.initials: K
authors.lastname: Bergström
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D002908:Chronic Disease; D004926:Escherichia coli; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D049449:Luminescence; D008297:Male; D008875:Middle Aged; D018375:Neutrophil Activation / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D010518:Periodontitis / Q000276:immunology*; D011110:Polymorphism, Genetic / Q000235:genetics; D017382:Reactive Oxygen Species / Q000032:analysis; D017452:Receptors, IgG / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000276:immunology
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha