title: Identification and comparative analysis of the large subunit mitochondrial ribosomal proteins of Neurospora crassa.
abstract: The mitochondrial ribosome (mitoribosome) has highly evolved from its putative prokaryotic ancestor and varies considerably from one organism to another. To gain further insights into its structural and evolutionary characteristics, we have purified and identified individual mitochondrial ribosomal proteins of Neurospora crassa by mass spectrometry and compared them with those of the budding yeast Saccharomyces cerevisiae. Most of the mitochondrial ribosomal proteins of the two fungi are well conserved with each other, although the degree of conservation varies to a large extent. One of the N. crassa mitochondrial ribosomal proteins was found to be homologous to yeast Mhr1p that is involved in homologous DNA recombination and genome maintenance in yeast mitochondria.
authors.affiliation: Department of Biology, Faculty of Science, Kobe University, Kobe, Japan.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gan
authors.forename: Kyoko
authors.initials: K
authors.lastname: Arita
authors.forename: Setsuko
authors.initials: S
authors.lastname: Isono
authors.forename: Madoka
authors.initials: M
authors.lastname: Kitakawa
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Yoshino
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yonezawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kato
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Inoue
authors.forename: Katsumi
authors.initials: K
authors.lastname: Isono
mesh_terms: D006801:Humans; D013058:Mass Spectrometry; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure*; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: C417727:MHR1 protein, S cerevisiae; D009687:Nuclear Proteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors