Expression of Bcl-x in normal skin and benign cutaneous adnexal tumors.
BACKGROUND
Bcl-x appears to have an antiapoptotic role in the epidermis. Little is known about the expression of Bcl-x in cutaneous adnexal structures and benign cutaneous adnexal tumors.


METHODS
Tissues from 31 cases of benign cutaneous adnexal tumors (five trichofolliculomas, five trichoepitheliomas, two sebaceous adenomas, five apocrine hidradenomas, five eccrine poromas, five eccrine spiradenomas, and four syringomas) were immunostained for Bcl-x.


RESULTS
Strong staining for Bcl-x was seen in cells of the epidermal granular layer and inner root sheath of hair follicles. Sebaceous gland cells showed strong staining. Apocrine gland cells showed weak to moderate staining. No staining was seen in eccrine gland cells. The basaloid cells of trichofolliculomas and trichoepitheliomas showed no staining. In sebaceous adenomas, the sebaceous cells showed strong staining while the basaloid cells were negative. The cells of apocrine hidradenomas showed patchy weak staining. No staining was seen in eccrine poromas, eccrine spiradenomas, and syringomas.


CONCLUSIONS
The degree of Bcl-x expression in cutaneous adnexal glandular structures appears to be related to their mode of secretion, being strongest in cells with apoptotic degradation of the entire cell (sebocytes). This pattern is recapitulated in the corresponding benign cutaneous adnexal tumors. Bcl-x may be useful in identifying cells with sebaceous differentiation in poorly differentiated adnexal tumors.
Thamboo
Thomas P
TP
Department of Pathology, National University of Singapore, Singapore. pattpt@nus.edu.sg
Tan
Leonard H-C
LH
Tan
Soo-Yong
SY
D014408:Biomarkers, Tumor / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D018294:Neoplasms, Adnexal and Skin Appendage / Q000378:metabolism* / Q000473:pathology; D012627:Sebaceous Glands / Q000378:metabolism / Q000473:pathology; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology; D013545:Sweat Glands / Q000378:metabolism / Q000473:pathology; D051020:bcl-X Protein / Q000378:metabolism*
D014408:Biomarkers, Tumor; D051020:bcl-X Protein