title: Laboratory colonization of Aedes lineatopennis.
abstract: Aedes lineatopennis, a species member of the subgenus Neomelaniconion, could be colonized for more than 10 successive generations from 30 egg batches [totally 2,075 (34-98) eggs] of wild-caught females. The oviposited eggs needed to be incubated in a moisture chamber for at least 7 days to complete embryonation and, following immersion in 0.25-2% hay-fermented water, 61-66% of them hatched after hatching stimulation. Larvae were easily reared in 0.25-1% hay-fermented water, with suspended powder of equal weight of wheat germ, dry yeast, and oatmeal provided as food. Larval development was complete after 4-6 days. The pupal stage lasted 3-4 days when nearly all pupae reached the adult stage (87-91%). The adults had to mate artificially, and 5-day-old males proved to be the best age for induced copulation. Three to five-day-old females, which were kept in a paper cup, were fed easily on blood from an anesthetized golden hamster that was placed on the top-screen. The average number of eggs per gravid female was 63.56 +/- 22.93 (22-110). Unfed females and males, which were kept in a paper cup and fed on 5% multivitamin syrup solution, lived up to 43.17 +/- 12.63 (9-69) and 15.90 +/- 7.24 (2-39) days, respectively, in insectarium conditions of 27 +/- 2 degrees C and 70-80% relative humidity.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Atchariya
authors.initials: A
authors.lastname: Jitpakdi
authors.forename: Anuluck
authors.initials: A
authors.lastname: Junkum
authors.forename: Benjawan
authors.initials: B
authors.lastname: Pitasawat
authors.forename: Narumon
authors.initials: N
authors.lastname: Komalamisra
authors.forename: Eumporn
authors.initials: E
authors.lastname: Rattanachanpichai
authors.forename: Udom
authors.initials: U
authors.lastname: Chaithong
authors.forename: Pongsri
authors.initials: P
authors.lastname: Tippawangkosol
authors.forename: Kom
authors.initials: K
authors.lastname: Sukontason
authors.forename: Natee
authors.initials: N
authors.lastname: Puangmalee
authors.forename: Wej
authors.initials: W
authors.lastname: Choochote
mesh_terms: D000330:Aedes / Q000254:growth & development*; D000818:Animals; D018554:Avena; D005247:Feeding Behavior; D005260:Female; D005285:Fermentation; D005656:Fungal Proteins; D007753:Laboratories; D007814:Larva / Q000254:growth & development*; D008297:Male; D010058:Oviposition; D013045:Species Specificity; D014908:Triticum
chemical_list: D005656:Fungal Proteins