title: Neuregulin regulates the formation of radial glial scaffold in hippocampal dentate gyrus of postnatal rats.
abstract: In the rodent hippocampus, the radial glial scaffold consists of radial glial cells (RGCs) and plays important roles in neurogenesis in this area after birth. However, the mechanisms that maintain the radial glial scaffold in the postnatal dentate gyrus (DG) area remain elusive. In the present work, we studied the role of Neuregulin (NRG) in the formation and maintenance of the radial glial scaffold in the hippocampal DG of postnatal rats using slice culture. We found that ErbB4 receptors were expressed in vimentin-positive RGCs in DG of postnatal day 6 (P6) rats. Treatment with NRG and Ab-3, the inhibitor of ErbB4, revealed that in P6 rats exogenous NRG promoted the proliferation of Vimentin-positive RGCs in DG. On the other hand, endogenous NRG was found necessary for maintaining the characteristic morphological and immunohistochemical features of these cells. These results indicated that NRG plays a critical role in the formation and maintenance of the radial glial scaffold in the hippocampal DG of postnatal rats.
authors.affiliation: Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Chang-Hong
authors.initials: CH
authors.lastname: Zheng
authors.forename: Linyin
authors.initials: L
authors.lastname: Feng
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D066246:ErbB Receptors / Q000032:analysis; D007381:Intermediate Filament Proteins / Q000032:analysis; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis; D064231:Nestin; D020880:Neuregulins / Q000494:pharmacology*; D009457:Neuroglia / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D020534:Parahippocampal Gyrus / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D010446:Peptide Fragments / Q000235:genetics / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D066247:Receptor, ErbB-4; D011994:Recombinant Proteins / Q000494:pharmacology; D046509:Tissue Culture Techniques / Q000379:methods; D014746:Vimentin / Q000032:analysis
chemical_list: D066246:ErbB Receptors; D007381:Intermediate Filament Proteins; D020929:Mitogen-Activated Protein Kinase Kinases; D009419:Nerve Tissue Proteins; D064231:Nestin; D020880:Neuregulins; D010446:Peptide Fragments; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors; D066247:Receptor, ErbB-4; D011994:Recombinant Proteins; D014746:Vimentin; C577486:Nes protein, rat; C587691:Erbb4 protein, rat