title: Feasibility of using papaya skin extract for digestion of swamp eel viscera for harvesting infective stage larva of Gnathostoma spp.
abstract: In this study, we reported the feasibility of using papaya skin extract (Carica papaya L.) as an alternative to enzyme pepsin in harvesting Gnathostoma spinigerum third-stage larvae. From experimental digestion, we found that the different numbers of recovered larvae between papaya skin extract and pepsin were not statistically significant (p >0.05). When the derived larvae from pepsin and papaya skin extract digestion were cultivated in BME medium for 7 days, the survival rates were not significantly different either (p >0.05). Thus, papaya skin extract might be another choice for recovering Gnathostoma spinigerum third-stage larvae.
authors.affiliation: Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand. ssuphan@chula.ac.th
authors.forename: Suphan
authors.initials: S
authors.lastname: Soogarun
authors.forename: Thamaporn
authors.initials: T
authors.lastname: Lertlum
authors.forename: Jamsai
authors.initials: J
authors.lastname: Suwansaksri
authors.forename: Viroj
authors.initials: V
authors.lastname: Wiwanitkit
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D029441:Carica / Q000378:metabolism*; D004063:Digestion; D004524:Eels / Q000469:parasitology*; D005240:Feasibility Studies; D006039:Gnathostoma / Q000302:isolation & purification*; D006801:Humans; D007814:Larva; D008099:Liver / Q000469:parasitology*; D010434:Pepsin A / Q000378:metabolism; D010936:Plant Extracts / Q000378:metabolism*; D017205:Spirurida Infections / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D010936:Plant Extracts; D010434:Pepsin A