[NP9 gene inhibits tumorigenicity of nasopharyngeal carcinoma].
OBJECTIVE
To study the effect of NP9 on the growth of transplanted nasopharyngeal carcinoma (NPC) in nude mice and explore the mechanisms involved.


METHODS
Recombinant pRc/CMV2-NP9 plasmid was constructed and transfected into the NPC cell lines by lipofectamine 2000. Cell clones stably expressing NP9 were obtained by detecting the mRNA expression of NP9 in G418-resistant clones with RT-PCR. The tumorigenicity and size of transplanted tumors were assessed after inoculation of NPC cells and their transgene clones into Balb/C mice. The expression of PCNA and cyclin D1 in transplanted tumors was detected by immunohistochemistry.


RESULTS
The expression of NP9 was detected in some of NP9 gene-transfected G418-resistant clones of CNE1 and SUNE1. In vivo experiments showed that the tumorigenicity of CNE19 clone was decreased significantly compared to that of CNE1 and its vector control, and the transplanted tumors grew more slowly from SUNE1/NP9 than from SUNE1 and SUNE1/vector. Compared with the vector control, the expression of cyclin D1 and PCNA in CNE1/NP9 transplants was decreased.


CONCLUSIONS
NP9 inhibits tumorigenicity and growth of NPC transplanted tumor by down-regulating the expression of cyclin D1 and PCNA.
Liu
Qi-cai
QC
Experiment Medical Research Center, Guangzhou Medical College, Guangzhou 510182, China.
Li
Xiao-yan
XY
Wei
Ba-xiong
BX
Zeng
Yi-xin
YX
D000818:Animals; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics; D020077:Endogenous Retroviruses / Q000235:genetics*; D005260:Female; D015686:Gene Products, env / Q000096:biosynthesis / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009368:Neoplasm Transplantation; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
D015686:Gene Products, env; C469430:Np9 protein, HERV-K, human; D018809:Proliferating Cell Nuclear Antigen; D019938:Cyclin D1