Prediction value of radiosensitivity of hepatocarcinoma cells for apoptosis and micronucleus assay.
OBJECTIVE
To investigate the prediction value of radiosensitivity of hepatocarcinoma cells for apoptosis and micronucleus assay.


METHODS
Clonogenic assay, flow cytometry, and CB micronuclei assay were used to survey the cell survival rate, radiation-induced apoptosis and micronucleus frequency of hepatocarcinoma cell lines SMMC-7721, HL-7702, and HepG2 after being irradiated by X-ray at the dosage ranging 0-8 Gy.


RESULTS
After irradiation, there was a dose-effect relationship between micronucleus frequency and radiation dosage among the three cell lines (P<0.05). A positive relationship was observed between apoptosis and radiation dosage among the three cell lines. The HepG2 cells had a significant correlation (P<0.05) but apoptosis incidence had a negative relationship with micronucleus frequency. There was a positive relationship between apoptosis and radiation dosage and the correlation between SMMC-7721 and HL-7702 cell lines had a significant difference (P<0.01). After irradiation, a negative relationship between cell survival rate and radiation dosages was found among the three cell lines (P<0.01). There was a positive relationship between cell survival rate and micronucleus frequency (P<0.01). No correlation was observed between apoptosis and cell survival rate.


CONCLUSIONS
The radiosensitivity of hepatocarcinoma cells can be reflected by apoptosis and micronuclei. Detection of apoptosis and micronuclei could enhance the accuracy for predicting radiosensitivity.
Liu
Zhi-Zhong
ZZ
Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. whzzl@163.net
Huang
Wen-Ying
WY
Li
Xiao-Sheng
XS
Lin
Ju-Sheng
JS
Cai
Xiao-Kun
XK
Lian
Kuo-Huang
KH
Zhou
He-Jun
HJ
D017209:Apoptosis; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D002470:Cell Survival; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008113:Liver Neoplasms; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D011237:Predictive Value of Tests; D011836:Radiation Tolerance; D014965:X-Rays