Outer membrane vesicles from group B Neisseria meningitidis delta gna33 mutant: proteomic and immunological comparison with detergent-derived outer membrane vesicles.
We compared the proteome of detergent-derived group B Neisseria meningitidis (MenB) outer membrane vesicles (DOMVs) with the proteome of outer membrane vesicles (m-OMVs) spontaneously released into culture supernatant by MenB delta gna33, a mutant in which the gene coding for a lytic transglycosylase homologous to the E. coli MltA was deleted. In total, 138 proteins were identified in DOMVs by 1- and 2-DE coupled with MS; 64% of these proteins belonged to the inner membrane and cytoplasmic compartments. By contrast, most of the 60 proteins of m-OMVs were classified by PSORT as outer membrane proteins. When tested for their capacity to elicit bactericidal antibodies, m-OMVs elicited a broad protective activity against a large panel of MenB strains. Therefore, the identification of mutations capable of conferring an OMV-releasing phenotype in bacteria may represent an attractive approach to study bacterial membrane composition and organization, and to design new efficacious vaccine formulations.
Ferrari
Germano
G
Biochemistry and Molecular Biology Unit, Chiron Vaccines, Siena, Italy.
Garaguso
Ignazio
I
Adu-Bobie
Jeannette
J
Doro
Francesco
F
Taddei
Anna Rita
AR
Biolchi
Alessia
A
Brunelli
Brunella
B
Giuliani
Marzia Monica
MM
Pizza
Mariagrazia
M
Norais
Nathalie
N
Grandi
Guido
G
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure; D002850:Chromatography, Gel; D019295:Computational Biology; D003902:Detergents / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005434:Flow Cytometry; D017353:Gene Deletion; D013058:Mass Spectrometry; D038541:Neisseria meningitidis, Serogroup B / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D012703:Serotyping
D001425:Bacterial Outer Membrane Proteins; D003902:Detergents