Characterization and expression analysis during embryo development of the mouse ortholog of MLL3.
We characterized the mouse ortholog of the human MLL3 gene and a 10.6 kb-Mll3 transcript. The mouse Mll3 gene comprises 60 exons that encompass 226 kb in chromosome 5. The predicted protein of 3464 amino acids contains two PHD domains, an ATPase alpha_beta signature, an HMG, and a SET domain. We analyzed the expression of the Mll3 gene during the embryonic development of the mouse by whole-mount in situ hybridization. Low levels of expression throughout the embryo were first detected at 8.0 dpc. At this stage, the signal was already stronger in the forebrain neuroepithelium and absent in the heart. Next, expression outlined the ventral neural tube, the somites, the limbs, and the eye lens remaining at low levels throughout the embryo. By 13.0 dpc, expression became stronger in the spinal cord, in hand/foot plates, and in gonads. RT-PCR confirmed that Mll3 is expressed early during gametogenesis. We suggest that Mll3 is expressed early in pre-spermatogonia and then in spermatogonia.
Brun
Marie-Elisabeth
ME
Plasticité Génomique, Chromatine et Cancer, Institut de Génétique Humaine, CNRS UPR 1142, 141, rue de la Cardonille, 34396 Montpellier, France.
Gasca
Stéphan
S
Girard
Cyrille
C
Bouton
Katia
K
De Massy
Bernard
B
De Sario
Albertina
A
D000818:Animals; D005121:Extremities / Q000196:embryology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D011495:Histone-Lysine N-Methyltransferase / Q000096:biosynthesis* / Q000235:genetics; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D051379:Mice; D051788:Myeloid-Lymphoid Leukemia Protein / Q000096:biosynthesis* / Q000235:genetics; D009928:Organ Specificity / Q000502:physiology; D013091:Spermatogenesis / Q000502:physiology*; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology*; D013737:Testis / Q000166:cytology / Q000196:embryology*; D004268:DNA-Binding Proteins
D011495:Histone-Lysine N-Methyltransferase; D051788:Myeloid-Lymphoid Leukemia Protein; C510652:Kmt2c protein, mouse; D004268:DNA-Binding Proteins