title: Two new colorimetric methods for early detection of vancomycin and oxacillin resistance in Staphylococcus aureus.
abstract: We developed two colorimetric methods for the detection of vancomycin- and oxacillin-resistant Staphylococcus aureus in </=6 h: (i) a nitrate reductase assay and (ii) a resazurin microplate method. MICs agreed with results obtained by CLSI methods for oxacillin. However, detection of vancomycin resistance required a larger inoculum. These methods may be recommended for the detection of vancomycin- and oxacillin-resistant S. aureus.
authors.affiliation: Department of Pathology, Hershey Medical Center, P.O. Box 850, Hershey, PA 17033, USA.
authors.forename: Ahmet Y
authors.initials: AY
authors.lastname: Coban
authors.forename: Bülent
authors.initials: B
authors.lastname: Bozdogan
authors.forename: Cigdem Cekic
authors.initials: CC
authors.lastname: Cihan
authors.forename: Ebru
authors.initials: E
authors.lastname: Cetinkaya
authors.forename: Kemal
authors.initials: K
authors.lastname: Bilgin
authors.forename: Ozge
authors.initials: O
authors.lastname: Darka
authors.forename: Alper
authors.initials: A
authors.lastname: Akgunes
authors.forename: Belma
authors.initials: B
authors.lastname: Durupinar
authors.forename: Peter C
authors.initials: PC
authors.lastname: Appelbaum
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003124:Colorimetry / Q000379:methods; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D050901:Nitrate Reductase / Q000378:metabolism*; D010068:Oxacillin / Q000494:pharmacology*; D010078:Oxazines / Q000378:metabolism*; D010403:Penicillin Resistance; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism; D020713:Vancomycin Resistance; D014966:Xanthenes / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D050901:Nitrate Reductase; D010068:Oxacillin