title: Differential effects of iontophoretic in vivo application of the GABA(A)-antagonists bicuculline and gabazine in sensory cortex.
abstract: We have compared the effects of microiontophoretic application of the GABA(A)-receptor antagonists bicuculline (BIC) and gabazine (SR95531) on responses to pure tones and to sinusoidally amplitude-modulated (AM) tones in cells recorded extracellularly from primary auditory cortex (AI) of Mongolian gerbils. Besides similar effects in increasing spontaneous and stimulus-evoked activity and their duration, both drugs elicited differential effects on spectral tuning and synchronized responses to AM tones. In contrast to gabazine, iontophoresis of the less potent GABA(A)-antagonist BIC often resulted in substantial broadening of frequency tuning for pure tones and an elimination of synchronized responses to AM tones, particularly with high ejecting currents. BIC-induced effects which could not be replicated by application of gabazine were presumably due to the well-documented, non-GABAergic side-effects of BIC on calcium-dependent potassium channels. Our results thus provide strong evidence that GABA(A)-mediated inhibition in AI does not sharpen frequency tuning for pure tones, but rather contributes to the processing of fast temporal modulations of sound envelopes. They also demonstrate that BIC can have effects on neuronal response selectivity which are not due to blockade of GABAergic inhibition. The results have profound implications for microiontophoretic studies of the role of intracortical inhibition in sensory cortex.
authors.affiliation: Leibniz Institute for Neurobiology, Brenneckestrasse 6, 39118 Magdeburg, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Kurt
authors.forename: John M
authors.initials: JM
authors.lastname: Crook
authors.forename: Frank W
authors.initials: FW
authors.lastname: Ohl
authors.forename: Henning
authors.initials: H
authors.lastname: Scheich
authors.forename: Holger
authors.initials: H
authors.lastname: Schulze
mesh_terms: D000161:Acoustic Stimulation / Q000379:methods; D000818:Animals; D001303:Auditory Cortex / Q000187:drug effects* / Q000502:physiology; D001307:Auditory Perception / Q000187:drug effects* / Q000502:physiology; D001640:Bicuculline / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018756:GABA Antagonists / Q000008:administration & dosage / Q000494:pharmacology*; D058787:GABA-A Receptor Antagonists; D005849:Gerbillinae; D007478:Iontophoresis; D008297:Male; D009474:Neurons / Q000187:drug effects; D011724:Pyridazines / Q000008:administration & dosage / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D018756:GABA Antagonists; D058787:GABA-A Receptor Antagonists; D011724:Pyridazines; D005680:gamma-Aminobutyric Acid; C049853:gabazine; D001640:Bicuculline