Colonization of tomato root by pathogenic and nonpathogenic Fusarium oxysporum strains inoculated together and separately into the soil.
In soil, fungal colonization of plant roots has been traditionally studied by indirect methods such as microbial isolation that do not enable direct observation of infection sites or of interactions between fungal pathogens and their antagonists. Confocal laser scanning microscopy was used to visualize the colonization of tomato roots in heat-treated soil and to observe the interactions between a nonpathogenic strain, Fo47, and a pathogenic strain, Fol8, inoculated onto tomato roots in soil. When inoculated separately, both fungi colonized the entire root surface, with the exception of the apical zone. When both strains were introduced together, they both colonized the root surface and were observed at the same locations. When Fo47 was introduced at a higher concentration than Fol8, it colonized much of the root surface, but hyphae of Fol8 could still be observed at the same location on the root. There was no exclusion of the pathogenic strain by the presence of the nonpathogenic strain. These results are not consistent with the hypothesis that specific infection sites exist on the root for Fusarium oxysporum and instead support the hypothesis that competition occurs for nutrients rather than for infection sites.
Olivain
Chantal
C
UMR Microbiologie Géochimie des Sols INRA, BP 86510, F-21065 Dijon Cedex, France.
Humbert
Claude
C
Nahalkova
Jarmila
J
Fatehi
Jamshid
J
L'Haridon
Floriane
F
Alabouvette
Claude
C
D005670:Fusarium / Q000254:growth & development / Q000472:pathogenicity*; D018551:Solanum lycopersicum / Q000254:growth & development / Q000382:microbiology*; D018613:Microscopy, Confocal; D010935:Plant Diseases / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D012988:Soil Microbiology; D013045:Species Specificity; D014774:Virulence