title: Activation of the hedgehog pathway in a subset of lung cancers.
abstract: Activation of the hedgehog pathway is reported in lung cancer, but its frequency remains unknown. We examine activation of this pathway in lung cancers by in situ hybridization and immunohistochemstry, and find that less than 10% of the tumors have elevated hedgehog target gene expression. We further identify a cell line NCI-H209 and two primary tumors with no detectable Su(Fu), a negative regulator of the pathway. Ectopic expression of Su(Fu) in NCI-H209 cells down-regulates hedgehog target gene expression and leads to inhibition of cell proliferation. These data indicate that activation of the hedgehog pathway is activated through Shh over-expression or Su(Fu) inactivation in only a subset of lung cancers.
authors.affiliation: Department of Pharmacology, Sealy Center for Cancer Cell Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1048, USA.
authors.forename: Sumin
authors.initials: S
authors.lastname: Chi
authors.forename: Shuhong
authors.initials: S
authors.lastname: Huang
authors.forename: Chengxin
authors.initials: C
authors.lastname: Li
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.forename: Nonggao
authors.initials: N
authors.lastname: He
authors.forename: Manoop S
authors.initials: MS
authors.lastname: Bhutani
authors.forename: Dennie
authors.initials: D
authors.lastname: Jones
authors.forename: Claudia Y
authors.initials: CY
authors.lastname: Castro
authors.forename: Roberto
authors.initials: R
authors.lastname: Logrono
authors.forename: Abida
authors.initials: A
authors.lastname: Haque
authors.forename: Joseph
authors.initials: J
authors.lastname: Zwischenberger
authors.forename: Stephen K
authors.initials: SK
authors.lastname: Tyring
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.forename: Jingwu
authors.initials: J
authors.lastname: Xie
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D018287:Carcinoma, Large Cell / Q000235:genetics / Q000378:metabolism; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D003114:Colony-Forming Units Assay; D005260:Female; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D008297:Male; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D000072079:Patched Receptors; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000071676:Zinc Finger Protein GLI1
chemical_list: D053823:Hedgehog Proteins; D015513:Oncogene Proteins; D000072079:Patched Receptors; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011956:Receptors, Cell Surface; D012097:Repressor Proteins; C490764:SUFU protein, human; D015534:Trans-Activators; D000071676:Zinc Finger Protein GLI1