Reverse serial analysis of gene expression (SAGE) characterization of orphan SAGE tags from human embryonic stem cells identifies the presence of novel transcripts and antisense transcription of key pluripotency genes.
Serial analysis of gene expression (SAGE) is a powerful technique for the analysis of gene expression. A significant portion of SAGE tags, designated as orphan tags, however, cannot be reliably assigned to known transcripts. We used an improved reverse SAGE (rSAGE) strategy to convert human embryonic stem cell (hESC)-specific orphan SAGE tags into longer 3' cDNAs. We show that the systematic analysis of these 3' cDNAs permitted the discovery of hESC-specific novel transcripts and cis-natural antisense transcripts (cis-NATs) and improved the assignment of SAGE tags that resulted from splice variants, insertion/deletion, and single-nucleotide polymorphisms. More importantly, this is the first description of cis-NATs for several key pluripotency markers in hESCs and mouse embryonic stem cells, suggesting that the formation of short interfering RNA could be an important regulatory mechanism. A systematic large-scale analysis of the remaining orphan SAGE tags in the hESC SAGE libraries by rSAGE or other 3' cDNA extension strategies should unravel additional novel transcripts and cis-NATs that are specifically expressed in hESCs. Besides contributing to the complete catalog of human transcripts, many of them should prove to be a valuable resource for the elucidation of the molecular pathways involved in the self-renewal and lineage commitment of hESCs.
Richards
Mark
M
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543.
Tan
Siew-Peng
SP
Chan
Woon-Khiong
WK
Bongso
Ariff
A
D002478:Cells, Cultured; D016373:DNA, Antisense / Q000235:genetics*; D004622:Embryo, Mammalian / Q000166:cytology; D020869:Gene Expression Profiling / Q000379:methods; D015723:Gene Library; D006801:Humans; D039904:Pluripotent Stem Cells / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis; D016324:Sequence Tagged Sites; D015533:Transcriptional Activation
D016373:DNA, Antisense; D012313:RNA