title: Synergistic interactions of ferulic acid with ascorbic acid: its cardioprotective role during isoproterenol induced myocardial infarction in rats.
abstract: Studies on the lipid peroxidation and antioxidant changes and their significance during myocardial injury have provided a new insight into the pathogenesis of heart disease. The heart failure subsequent to myocardial infarction may be associated with an antioxidant deficit as well as increased myocardial oxidative stress. The present study was designed to evaluate the effect of the combination of ferulic acid and ascorbic acid on antioxidant defense system and lipid peroxidation against isoproterenol (ISO)-induced myocardial infarction in rats. Induction of rats with isoproterenol (150 mg/kg body weight daily, i.p.) for 2 days resulted in a marked elevation in lipid peroxidation, serum marker enzymes (LDH, CPK, GOT, and GPT), and a significant decrease in activities of endogenous antioxidants (SOD, GPx, GST, CAT, and GSH). Pre-co-treatment with the combination of ferulic acid (20 mg/kg body weight/day) and ascorbic acid (80 mg/kg body weight/day) orally for 6 days, significantly attenuated these changes when compared to the individual treatment groups. Histopathological observations were also in correlation with the biochemical parameters. Thus, ferulic acid and ascorbic acid significantly counteracted the pronounced oxidative stress effect of ISO by the inhibition of lipid peroxidation, restoration of antioxidant status, and myocardial marker enzymes levels. In conclusion, these findings indicate the synergistic protective effect of ferulic acid and ascorbic acid on lipid peroxidation and antioxidant defense system during ISO-induced myocardial infarction and associated oxidative stress in rats.
authors.affiliation: Department of Biochemistry, University of Madras, Guindy Campus, Chennai, Tamilnadu, India.
authors.forename: Surinder Kumar
authors.initials: SK
authors.lastname: Yogeeta
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Gnanapragasam
authors.forename: Subramanian Senthil
authors.initials: SS
authors.lastname: Kumar
authors.forename: Rajakannu
authors.initials: R
authors.lastname: Subhashini
authors.forename: Arumugam
authors.initials: A
authors.lastname: Sathivel
authors.forename: Thiruvengadum
authors.initials: T
authors.lastname: Devaki
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000627:therapeutic use*; D003373:Coumaric Acids / Q000627:therapeutic use*; D004347:Drug Interactions; D004357:Drug Synergism; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D007545:Isoproterenol / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; D016166:Free Radical Scavengers; C004999:ferulic acid; D010088:Oxidoreductases; D007545:Isoproterenol; D001205:Ascorbic Acid