Screening for allergic respiratory disease in the general population with the ADVIA Centaur Allergy Screen Assay.
BACKGROUND
In patients in whom the clinical indication for immunoglobulin E (IgE)-mediated allergic respiratory disease is weak, a single qualitative multiallergen-screening assay for IgE antibody to multiple allergen specificities may support the absence of IgE-mediated allergic respiratory disease. The aim was to investigate the diagnostic efficacy of a new multiallergen-screening assay in relation to skin prick test (SPT) reactivity and objective diagnoses of allergic respiratory disease in a general population setting.


METHODS
A total of 709 participants in a population-based study were examined by questionnaire and SPT. Serum was analysed by using a multiallergen-screening assay: the ADVIA Centaur Allergy Screen (AS) assay. The dichotomized result of the AS assay was compared with SPT reactivity, specific IgE positivity, and a clinical diagnosis of allergic rhinitis or allergic asthma defined by the presence of relevant symptoms and positive SPTs.


RESULTS
Sensitivity, specificity, and positive (PPV) and negative predictive values (NPV) of the AS against SPT reactivity were 86%, 96%, 94%, and 89%, respectively. A negative AS assay test was able to exclude allergic rhinitis and allergic asthma with a probability of more than 96% and 98% (NPV), respectively. The AS assay was able to identify more than 92% and 92% (sensitivity) of cases of allergic rhinitis and allergic asthma, respectively.


CONCLUSIONS
The AS assay proved to be a valid measure of allergic respiratory disease and may be used as a screening tool to rule out allergic respiratory disease, and as an objective measure of allergic respiratory disease in epidemiological studies.
Linneberg
A
A
Research Centre for Prevention and Health, Glostrup University Hospital, Glostrup, Denmark.
Husemoen
L L N
LL
Nielsen
N H
NH
Madsen
F
F
Frølund
L
L
Johansen
N
N
D000888:Antibodies, Anti-Idiotypic / Q000097:blood*; D001249:Asthma / Q000175:diagnosis / Q000453:epidemiology; D015331:Cohort Studies; D016001:Confidence Intervals; D003888:Desensitization, Immunologic; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000453:epidemiology; D007073:Immunoglobulin E / Q000276:immunology*; D007159:Immunologic Tests / Q000379:methods; D008297:Male; D008403:Mass Screening / Q000379:methods*; D015995:Prevalence; D011336:Probability; D011446:Prospective Studies; D012130:Respiratory Hypersensitivity / Q000175:diagnosis* / Q000453:epidemiology; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis / Q000453:epidemiology; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D012882:Skin Tests / Q000379:methods
D000888:Antibodies, Anti-Idiotypic; D007073:Immunoglobulin E