title: A transformation system for the hypotrichous ciliate Stylonychia mytilus.
abstract: We describe a transformation system for the ciliate Stylonychia mytilus. The neomycin resistance gene from Escherichia coli transposon Tn5, which codes for the enzyme phosphotransferase and confers resistance to the antibiotic G 418, was ligated into macronuclear ;gene-size' DNA molecules. Using this recombinant DNA for transformation experiments we show that the gene is replicated and expressed in transformed cells.
authors.affiliation: Institut für Biologie III, Abt. Zellbiologie, Universität Tübingen, Auf der Morgenstelle 28, D-7400 Tübingen, FRG.
authors.forename: I U
authors.initials: IU
authors.lastname: Wünning
authors.forename: H J
authors.initials: HJ
authors.lastname: Lipps