Determination of platelet-activating factor by reverse phase high-performance liquid chromatography and its application in viral hepatitis.
OBJECTIVE
To detect the platelet-activating factor (PAF) and the plasma or serum levels of tumor necrosis factor-alpha (TNF-alpha) malondialdehyde (MDA), endotoxin (ET) and to discuss their significance in various types of viral hepatitis.


METHODS
PAF, TNF-alpha, MDA, and ET levels in 60 controls, 16 cases of acute viral hepatitis, 71 cases of chronic viral hepatitis, 19 cases of severe viral hepatitis were detected by reverse phase high-performance liquid chromatography (rHPLC), bio-assay, ELISA, thiobarbituric acid (TBA), and limulus lysate test (LLT), respectively.


RESULTS
The rHPLC was more sensitive and specific than bio-assay (r = 0.912, P<0.01). The plasma levels of PAF, TNF-alpha, MDA, and ET in patients with viral hepatitis were higher than those in controls (P<0.01).


CONCLUSIONS
rHPLC is more reliable and accurate for the detection of PAF.
Cao
Hong-Cui
HC
Department of Infectious Disease, First Affiliated Hospital, Medical School of Zhejiang University, Zhejiang Province, China.
Chen
Xiao-Ming
XM
Xu
Wei
W
D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods / Q000706:statistics & numerical data; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000706:statistics & numerical data; D004731:Endotoxins / Q000097:blood; D005260:Female; D006525:Hepatitis, Viral, Human / Q000097:blood*; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D010972:Platelet Activating Factor / Q000032:analysis*; D012680:Sensitivity and Specificity; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D004731:Endotoxins; D010972:Platelet Activating Factor; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde