title: [Common bile duct ligation as a model of hepatopulmonary syndrome and oxidative stress].
abstract: BACKGROUND
  The hepatopulmonary syndrome is characterized by hepatic dysfunction and presence of dilated pulmonary vessels, with alterations in air diffusion that can be demonstrated in the experimental model of common bile duct ligation.
  
  
  OBJECTIVE
  To evaluate the oxidative stress in pulmonary tissue of cirrhotic rats with common bile duct ligation.
  
  
  METHODS
  We used 12 male Wistar rats weighing between 200-300 g divided in two groups: control (Co = 6) and cirrhotic (Ci = 6). We evaluated aminotransferases, arterial gasometry, lipoperoxidation and chemoluminescence), and antioxidant enzymatic activity with superoxide dismutase. The tissues analyzed for hepatopulmonary syndrome were cirrhotic liver and lung.
  
  
  RESULTS
  The animals with common bile duct ligation showed alterations in the following aminotransferases: aspartate aminotransferase, Co = 105.3 +/- 43/Ci = 500.5 +/- 90.3, alanine aminotransferase, Co = 78.75 +/- 37.7/Ci = 162.75 +/- 35.4, and alkaline phosphatase, Co = 160 +/- 20.45/Ci = 373 +/- 45.44. The lipoperoxidation and the antioxidant response had significant differences between the groups when evaluated in lung (lipoperoxidation) Co = 0.87 +/- 0.3/Ci = 2.01 +/- 0.9, chemoluminescence Co = 16008.41 +/- 1171.45/Ci = 20250.36 +/- 827.82 superoxide dismutase Co = 6.66 +/- 1.34/Ci = 16.06 +/- 2.67.
  
  
  CONCLUSIONS
  Our results suggest that in this experimental model of cirrhosis using common bile duct ligation, there is an increase in lipoperoxidation in pulmonary tissue as well as an increase in superoxide dismutase's antioxidant activity, suggesting a pulmonary injury caused by secondary biliary cirrhosis.
authors.affiliation: Laboratório de Fisiologia Digestiva e Estresse Oxidativo, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS.
authors.forename: Juliana
authors.initials: J
authors.lastname: Tieppo
authors.forename: Rafael
authors.initials: R
authors.lastname: Vercelino
authors.forename: Alexandre Simões
authors.initials: AS
authors.lastname: Dias
authors.forename: Cláudio Augusto
authors.initials: CA
authors.lastname: Marroni
authors.forename: Norma
authors.initials: N
authors.lastname: Marroni
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D003135:Common Bile Duct / Q000601:surgery*; D004195:Disease Models, Animal; D020065:Hepatopulmonary Syndrome / Q000201:enzymology / Q000209:etiology / Q000378:metabolism*; D008026:Ligation; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000032:analysis*; D017392:Thiobarbituric Acid Reactive Substances; D000637:Transaminases / Q000097:blood*
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase; D000637:Transaminases