title: Cloning and preliminary characterization of a GATC-specific beta2-class DNA:m6A methyltransferase encoded by transposon Tn1549 from Enterococcus spp.
abstract: A recent study revealed a subfamily of N6-adenine (m6A) methyltransferases that comprises a few functionally studied eukaryotic members acting on mRNA and prokaryotic members acting on DNA as well as numerous uncharacterized open reading frames. Here, we report cloning and functional characterization of a prokaryotic member of this family encoded by transposon Tn1549 from Enterococcus spp.
authors.affiliation: Institute of Microbiology, University of Warsaw, ul. Miecznikowa 1, 02-096 Warszawa, Poland. m.radlinska@biol.uw.edu.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Radlińska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Piekarowicz
authors.forename: Marc
authors.initials: M
authors.lastname: Galimand
authors.forename: Janusz M
authors.initials: JM
authors.lastname: Bujnicki
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016983:Enterococcus / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000737:chemistry / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)