title: Some factors affecting Stellantchasmus falcatus metacercaria in laboratory.
abstract: Killing factors, various temperatures and solutions were studied in the laboratory on Stellantchasmus falcatus metacercaria in half-beaked fish (Dermogenus pusillus). Killing criteria followed the Movability Index from 1.000 within 24 hours. The metacercariae were collected from Chiang Mai moat. They were incubated in 0.85 % NaCl at -20 degrees C, room temperature, 4 degrees, 37 degrees, and 65 degrees C. The in vitro investigation showed that at -20 degrees C and 65 degrees C, the worms were killed within 18 and 2 hours, respectively, while other temperatures produced no effect. The solutions investigated were NaCl (10, 20, 30, and 40%), lemon juice (25, 50, 75, and 100%), acetic acid (5, 10, 20, and 30%), vinegar (1, 3, and 5%) and water as a control. The worms were killed in NaCl at 20, 30, and 40% within 12, 6, and 2 hours, respectively. Acetic acid at 5% and 10% killed the metacercaria within 12 and 6 hours while at 20% and 30%, within 2 hours. The killing effect of 3% vinegar was found within 18 hours and of 5% vinegar within 12 hours. Lemon juice showed no killing effect.
authors.affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand. cwongsawd@yahoo.com
authors.forename: Chalobol
authors.initials: C
authors.lastname: Wongsawad
authors.forename: Siriwan
authors.initials: S
authors.lastname: Kawin
authors.forename: Pheravut
authors.initials: P
authors.lastname: Wongsawad
authors.forename: Thipmani
authors.initials: T
authors.lastname: Paratasilpin
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D000818:Animals; D005399:Fishes / Q000469:parasitology*; D005506:Food Contamination; D005511:Food Handling / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D009890:Opisthorchidae / Q000187:drug effects* / Q000302:isolation & purification; D017747:Seafood / Q000469:parasitology*; D012965:Sodium Chloride / Q000494:pharmacology; D013534:Survival; D014201:Trematode Infections / Q000175:diagnosis*
chemical_list: D012965:Sodium Chloride; D019342:Acetic Acid