title: Glycine application and right heart function in a porcine heart transplantation model.
abstract: Glycine reduces ischemia-reperfusion injury after experimental liver transplantation. We hypothesized that glycine might also protect right heart function in an isovolumic cardiac transplantation model. In six domestic donor pigs 150 ml of a 300 mmol L-glycine solution were administered intravenously. The hearts were then arrested with histidine-tryptophan-ketoglutarate solution. Animals without prior glycine infusion served as controls (n = 6). After 4 h of ischemia, hearts were transplanted into recipients. An isovolumic model was used in which the right ventricular (RV) volume was controlled in vivo using an intracavitary high-compliance balloon. After 1 and 2 h of reperfusion the RV balloon volume was gradually increased and the developed pressures were recorded (P(developed) = P(systolic) - P(diastolic)). Right ventricular failure was defined as a decrease in developed intracavitary pressure. Glycine hearts could be loaded with a significantly increased volume after 1 h (glycine: 53 +/- 13.7 ml vs. control: 32 +/- 11.7 ml; P = 0.015) and after 2 h (67 +/- 18.6 ml vs. 43 +/- 8.2 ml; P = 0.018). Maximal RV developed pressures were not significantly different between groups. Postischemic RV end-diastolic compliance was significantly higher in glycine-treated animals (P = 0.04). Glycine protects early postischemic RV compliance, but has no important influence on maximal developed pressures.
authors.affiliation: Division of Thoracic and Cardiovascular Surgery, Hannover Medical School, Hannover, Germany.
authors.forename: Gregor
authors.initials: G
authors.lastname: Warnecke
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schulze
authors.forename: Thomas
authors.initials: T
authors.lastname: Steinkamp
authors.forename: Axel
authors.initials: A
authors.lastname: Haverich
authors.forename: Uwe
authors.initials: U
authors.lastname: Klima
mesh_terms: D000818:Animals; D052279:Creatine Kinase, MB Form / Q000097:blood; D003971:Diastole; D005998:Glycine / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006321:Heart; D016027:Heart Transplantation / Q000379:methods*; D007511:Ischemia / Q000473:pathology; D016031:Liver Transplantation; D008953:Models, Anatomic; D015233:Models, Statistical; D009206:Myocardium / Q000473:pathology; D011312:Pressure; D015427:Reperfusion Injury; D013552:Swine; D013997:Time Factors; D020107:Troponin T / Q000097:blood; D018497:Ventricular Dysfunction, Right; D016278:Ventricular Function, Right; D017725:Ventricular Pressure
chemical_list: D020107:Troponin T; D052279:Creatine Kinase, MB Form; D005998:Glycine