Oxidative folding of conotoxins in immobilized systems.
We tested two alternative oxidation strategies to produce conotoxins alpha-GI and micro-PIIIA. The peptides were either reversibly immobilized on a solid support and then oxidized, or the immobilized disulfide reagent (CLEAR-OX(trade mark)) was used to oxidize the peptides. Both strategies appeared more efficient at higher peptide concentrations, consistent with pseudo-dilution effects.
Green
Brad R
BR
Cognetix, Inc., 421 Wakara Way, Suite 201, Salt Lake City, Utah 84108, USA.
Bulaj
Grzegorz
G
D000595:Amino Acid Sequence; D020916:Conotoxins / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017510:Protein Folding
D020916:Conotoxins; D004220:Disulfides