Mismatch tolerance by DNA polymerase Pol4 in the course of nonhomologous end joining in Saccharomyces cerevisiae.
In yeast, the nonhomologous end joining pathway (NHEJ) mobilizes the DNA polymerase Pol4 to repair DNA double-strand breaks when gap filling is required prior to ligation. Using telomere-telomere fusions caused by loss of the telomeric protein Rap1 and double-strand break repair on transformed DNA as assays for NHEJ between fully uncohesive ends, we show that Pol4 is able to extend a 3'-end whose last bases are mismatched, i.e., mispaired or unpaired, to the template strand.
Pardo
Benjamin
B
Laboratoire de Radiobiologie de l'ADN, Service de Radiobiologie Moléculaire et Cellulaire, UMR217 du CNRS, CEA/Fontenay, Fontenay-aux-Roses, France.
Ma
Emilie
E
Marcand
Stéphane
S
D000483:Alleles; D020137:Base Pair Mismatch; D001483:Base Sequence; D019951:DNA Polymerase beta; D017931:DNA Primers; D004260:DNA Repair; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000502:physiology*; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D017931:DNA Primers; C440829:RAP1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins; D014157:Transcription Factors; D019951:DNA Polymerase beta; D004259:DNA-Directed DNA Polymerase; C084892:POL4 protein, S cerevisiae