Transcriptional silencing of the mouse mammary tumor virus promoter through chromatin remodeling is concomitant with histone H1 phosphorylation and histone H3 hyperphosphorylation at M phase.
We examined histone phosphorylation and their effects on glucocorticoid receptor (GR)-mediated activation of the mouse mammary tumor virus promoter (MMTV) in synchronized cells. In vivo protein expression studies suggest that both histones H1 and H3 are highly phosphorylated in mitotic-arrested cells in which GR is unable to remodel chromatin and recruit transcription factor NF1 to the promoter. Postmitotic cells show an open chromatin structure and efficient binding of NF1 to the promoter accompanied by reversing histone H1 and H3 phosphorylation level. In contrast, the acetylation status of histone H3 and H4 did not change in either condition. These results suggest that hyperphosphorylation of histone H1 and H3 leads to inhibition of GR-mediated chromatin remodeling and inactivation of MMTV by preventing the association of transcription factors to the promoter in vivo.
Bhattacharjee
Rabindra N
RN
Department of Obstetrics and Gynaecology, University of Western Ontario, London, ON, Canada N6A 4L6. rabin@biken.osaka-u.ac.jp
Archer
Trevor K
TK
D000818:Animals; D002455:Cell Division; D045744:Cell Line, Tumor; D002843:Chromatin / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000502:physiology*; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D014158:Transcription, Genetic
D002843:Chromatin; D006657:Histones; D011965:Receptors, Glucocorticoid