title: Nucleotide variability of HV-I in admixed population of the Brazilian Amazon Region.
abstract: The analysis of genetic variation in the nucleotide sequences of mitochondrial DNA has been used as a tool in the study of history of different human populations, as Amerindians, Afro-descendents populations and furthermore admixed populations. In this study, the mitochondrial DNA was analyzed in 158 unrelated individuals in an admixed population of the Amazonian Region: Santarém-PA-Brazil. The polymorphisms were detected using both levels, analysis of restriction enzyme and direct sequencing. We observed a total of 49 different haplotypes were found determined by 46 variable nucleotides. The more frequent haplotypes (Hap03) was shared by five samples and 43 sequences were unique. The genetic diversity was estimated to 0.989+/-0.0067 and the probability of two random individuals showed identical mitochondrial DNA (mtDNA) haplotypes were 2.8%.
authors.affiliation: Laboratório de Genética Humana e Médica, Departamento de Patologia, Universidade Federal do Pará, Belém, Pará, Brazil.
authors.forename: Ana Cecília
authors.initials: AC
authors.lastname: Feio-Dos-Santos
authors.forename: Bruno Maia
authors.initials: BM
authors.lastname: Carvalho
authors.forename: Sidney Emanuel
authors.initials: SE
authors.lastname: Batista dos Santos
authors.forename: Andrea Kely Campos
authors.initials: AK
authors.lastname: Ribeiro-dos-Santos
mesh_terms: D001938:Brazil; D022801:Complementarity Determining Regions / Q000235:genetics*; D016172:DNA Fingerprinting; D004272:DNA, Mitochondrial / Q000235:genetics*; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial