Fast enantiomeric analysis of a complex essential oil with an innovative multidimensional gas chromatographic system.
The present research is focussed on the evaluation of a recently developed high performance multidimensional gas chromatographic (MDGC) system employed in the fast analysis of a series of chiral compounds contained in rosemary essential oil. The heart of the MDGC system consists in a simple transfer device for the rapid sequential re-injection of analyte "heart-cuts" from the first to the second dimension. The transfer system has no temperature restrictions, presents very low dead volumes and achieves multidimensional analysis through a pressure-balance mechanism. The MDGC set-up is characterized by two GC ovens (enabling independent temperature programming) and the possibility of mass spectrometric (MS) and/or flame ionization detection (FID). Multiple-cut conventional and fast MDGC-FID methods were developed and the results obtained compared, in order to evaluate the effectiveness of the system. In this respect, the rapid method provided the same analytical result in a greatly reduced time (approximately five times less). Furthermore, quali/quantitative data reproducibilty was very good. Fast MDGC was achieved by using micro-bore (0.1mm I.D.) columns in both dimensions.
Mondello
Luigi
L
Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università degli Studi di Messina, viale Annunziata, 98168 Messina, Italy. lmondello@pharma.unime.it
Casilli
Alessandro
A
Tranchida
Peter Quinto
PQ
Furukawa
Masanao
M
Komori
Kyoichi
K
Miseki
Kozo
K
Dugo
Paola
P
Dugo
Giovanni
G
D002849:Chromatography, Gas / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009822:Oils, Volatile / Q000737:chemistry*; D027542:Rosmarinus / Q000737:chemistry*; D013237:Stereoisomerism; D013729:Terpenes / Q000032:analysis*
D009822:Oils, Volatile; D013729:Terpenes; C053775:rosemary oil