Plane-wave fluorescence tomography with adaptive finite elements.
We present three-dimensional fluorescence yield tomography of a tissue phantom in a noncontact reflectance imaging setup. The method employs planar illumination with modulated light and frequency domain fluorescence measurements made on the illumination plane. An adaptive finite-element algorithm is used to handle the ill-posed and computationally demanding inverse image reconstruction problem. Tomographic images of fluorescent targets buried at 1-2 cm depths from the illumination surface demonstrate the feasibility of fluorescence tomography from reflectance tomography in clinically relevant tissue volumes.
Joshi
Amit
A
Photon Migration Laboratories, Department of Radiology, Baylor College of Medicine, Houston, Texas 77030, USA.
Bangerth
Wolfgang
W
Hwang
Kildong
K
Rasmussen
John
J
Sevick-Muraca
Eva M
EM
D000465:Algorithms; D003198:Computer Simulation; D020342:Finite Element Analysis; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008954:Models, Biological; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods*