title: Beta1,4-galactosyltransferase V functions as a positive growth regulator in glioma.
abstract: beta1,4-galactosyltransferase V (GalT V; EC 2.4.1.38) can effectively galactosylate the GlcNAcbeta1-->6Man arm of the highly branched N-glycans that are characteristic of glioma. Previously, we have reported that the expression of GalT V is increased in the process of glioma. However, currently little is known about the role of GalT V in this process. In this study, the ectopic expression of GalT V could promote the invasion and survival of glioma cells and transformed astrocytes. Furthermore, decreasing the expression of GalT V in glioma cells promoted apoptosis, inhibited the invasion and migration and the ability of tumor formation in vivo, and reduced the activation of AKT. In addition, the activity of GalT V promoter could be induced by epidermal growth factor, dominant active Ras, ERK1, JNK1, and constitutively active AKT. Taken together, our results suggest that GalT V functioned as a novel glioma growth activator and might represent a novel target in glioma therapy.
authors.affiliation: Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Gene Research Center, Shanghai Medical College of Fudan University (former Shanghai Medical University), Shanghai 200032, China.
authors.forename: Jianhai
authors.initials: J
authors.lastname: Jiang
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Chen
authors.forename: Jialin
authors.initials: J
authors.lastname: Shen
authors.forename: Yuanyan
authors.initials: Y
authors.lastname: Wei
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Yang
authors.forename: Hanzhou
authors.initials: H
authors.lastname: Wang
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zong
authors.forename: Junwu
authors.initials: J
authors.lastname: Yang
authors.forename: Si
authors.initials: S
authors.lastname: Zhang
authors.forename: Jianhui
authors.initials: J
authors.lastname: Xie
authors.forename: Xiangfei
authors.initials: X
authors.lastname: Kong
authors.forename: Weicheng
authors.initials: W
authors.lastname: Liu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Gu
mesh_terms: D017209:Apoptosis; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D004789:Enzyme Activation; D005700:Galactosyltransferases / Q000096:biosynthesis* / Q000378:metabolism*; D020869:Gene Expression Profiling; D005910:Glioma / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D009361:Neoplasm Invasiveness; D011401:Promoter Regions, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D005700:Galactosyltransferases; C419408:beta-1,4-galactosyltransferase V