In vitro and in vivo studies on CCR10 regulation by Annexin A1.
The mode of action of annexin A1 (ANXA1) is poorly understood. By using rapid subtraction hybridization we studied the effects of human recombinant ANXA1 and the N-terminal ANXA1 peptide on gene expression in a human larynx cell line. Three genes showed strong downregulation after treatment with ANXA1. In contrast, expression of CCR10, a seven transmembrane G-protein coupled receptor for chemokine CCL27 involved in mucosal immunity, was increased. Moreover the reduction in CCR10 expression induced by ANXA1 gene deletion was rescued by intravenous treatment with low doses of ANXA1. These findings provide new evidence that ANXA1 modulates gene expression.
Rodrigues-Lisoni
Flavia Cristina
FC
Department of Cellular and Molecular Neuroscience, Division of Neuroscience and Mental Health, Faculty of Medicine, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK.
Mehet
Devinder K
DK
Mehemet
Devinder K
DK
Peitl
Paulo
P
John
Christopher D
CD
da Silva Júnior
Wilson Araújo
WA
Tajara
Eloiza
E
Buckingham
Julia C
JC
Solito
Egle
E
D000818:Animals; D017305:Annexin A1 / Q000172:deficiency / Q000235:genetics*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007830:Larynx / Q000166:cytology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010446:Peptide Fragments / Q000494:pharmacology; D054447:Receptors, CCR10; D019707:Receptors, Chemokine / Q000502:physiology*; D011994:Recombinant Proteins
D017305:Annexin A1; C515706:CCR10 protein, human; C515707:Ccr10 protein, mouse; D010446:Peptide Fragments; D054447:Receptors, CCR10; D019707:Receptors, Chemokine; D011994:Recombinant Proteins