Autoantibodies in Argentine women with recurrent pregnancy loss.
OBJECTIVE
To determine the presence or absence of subclinical autoimmunity in Caucasian Argentine healthy women with first trimester recurrent pregnancy loss (RPL), the sera of 118 healthy women with a history of three or more consecutive abortions and 125 fertile control women without abortions and two children were analyzed for the presence of autoantibodies: immunoglobulin (Ig)G and IgM anticardiolipin, antinuclear (ANA), antismooth muscle (ASMA), antimitocondrial (AMA), antiliver-kidney-microsomal fraction (LKM), antigastric parietal cells (GPC), antineutrophil cytoplasmatic (ANCA) and antibodies antigliadin type IgA and IgG and IgA antitransglutaminase related with celiac disease (CD).


METHODS
ANA, ASMA, AMA, anti-LKM, antibodies to GPC and ANCA were determined by indirect immunofluorescence (IFI) and anticardiolipin, antigliadina and antitransglutaminase antibodies were measured by enzyme-linked immunosorbent assays (ELISA).


RESULTS
There was no significant difference between controls and patients with ANA, ASMA, AMA, LKM, ANCA and GPC. The prevalence of anticardiolipin antibodies in RPL was significantly higher than controls (P < 0,01) and the prevalence of positive antibodies for antigliadina type IgA and IgG and IgA antitransglutaminase in RPL was significantly higher than controls (P < 0.04).


CONCLUSIONS
We show that Caucasian Argentine women with RPL showed significantly higher incidence of anticardiolipin antibodies than normal controls and finally we recommended the screening of IgA and IgG antigliadina and IgA antitransglutaminase antibodies in pregnancy, because of the high prevalence of subclinical CD in RPL and the chance of reversibility through consumption of a gluten free diet.
Bustos
Daniel
D
Central Laboratory, Diagnostic Department, Hospital Italiano de Buenos Aires, Gascón 450, Buenos Aires, Argentina. daniel.bustos@hospitalitaliano.org.ar
Moret
Ana
A
Tambutti
Monica
M
Gogorza
Sebastian
S
Testa
Roberto
R
Ascione
Amanda
A
Prigoshin
Norma
N
D000026:Abortion, Habitual / Q000097:blood / Q000453:epidemiology / Q000276:immunology*; D000328:Adult; D001118:Argentina / Q000453:epidemiology; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008875:Middle Aged; D011247:Pregnancy
D001323:Autoantibodies