title: Interactions of high amylose starch and deoxycholic acid on gut functions in rats.
abstract: OBJECTIVE
  This study evaluated whether high amylose cornstarch (HAS) could prevent adverse physiologic effects induced by deoxycholic acid (DCA) in the gut of rats.
  
  
  METHODS
  Thirty-two male Sprague-Dawley rats were provided with a low amylose cornstarch (LAS) diet or a 50/50 mixture of LAS and HAS diets for 4 wk; each of these diets was supplemented with 0 or 2 g of DCA per kilogram of diet. Therefore, there were four groups.
  
  
  RESULTS
  Cecal content pH was lower in rats fed the HAS diets compared with rats fed the LAS diets (P < 0.05). Bifidobacteria number in cecal contents, cecal pools of acetate, butyrate, and total short-chain fatty acids were highest in rats fed the HAS diet; moreover, the HAS/DCA diet resulted in increased Bifidobacteria growth and short-chain fatty acid production numerically compared with the LAS/DCA diets (P = 0.06). Production of prostaglandin-E2 in colonic mucosa was highest in rats fed the LAS/DCA diet, and the intake of HAS significantly decreased prostaglandin-E2 levels (P < 0.05).
  
  
  CONCLUSIONS
  In this study, DCA may have inhibited fermentation in the large intestine and increased prostaglandin-E2 production, and concurrent administration of HAS and DCA may have decreased the adverse effects on the gut induced by DCA.
authors.affiliation: Department of Food and Life Science, Kookmin University, Seoul, Korea.
authors.forename: Moon Jeong
authors.initials: MJ
authors.lastname: Chang
authors.forename: So Mi
authors.initials: SM
authors.lastname: Soel
authors.forename: Myung Hee
authors.initials: MH
authors.lastname: Bang
authors.forename: Jung Han Yoon
authors.initials: JH
authors.lastname: Park
authors.forename: Nam E
authors.initials: NE
authors.lastname: Kang
authors.forename: Woo Kyoung
authors.initials: WK
authors.lastname: Kim
mesh_terms: D000688:Amylose / Q000008:administration & dosage / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D001644:Bifidobacterium / Q000254:growth & development* / Q000302:isolation & purification; D002432:Cecum / Q000737:chemistry / Q000382:microbiology; D015169:Colony Count, Microbial; D003840:Deoxycholic Acid / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005232:Fatty Acids, Volatile / Q000032:analysis*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013213:Starch / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D005232:Fatty Acids, Volatile; D003840:Deoxycholic Acid; D013213:Starch; D000688:Amylose; D015232:Dinoprostone