Xanthine oxidase inhibitors from the flowers of Chrysanthemum sinense.
From the MeOH extract of the flowers of Chrysanthemum sinense, a new flavone glucoside, acacetin 7- O-(3- O-acetyl- beta- D-glucopyranoside), has been isolated together with 27 known compounds including flavonoids, caffeoylquinic acid derivatives, phenolics, and a monoterpenoid glucoside. Their structures were elucidated on the basis of spectroscopic data. Compounds and displayed significant xanthine oxidase inhibitory activity in a concentration-dependent manner, and compounds and showed more potent inhibitory activity, with IC50 values ranging from 0.13 to 2.31 microM, than that of a positive control allopurinol (IC50=2.50 microM). The kinetic study indicated that and displayed competitive-type inhibition like that of allopurinol, while displayed a mixed-type inhibition.
Nguyen
Mai Thanh
MT
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
Awale
Suresh
S
Tezuka
Yasuhiro
Y
Ueda
Jun-ya
JY
Tran
Quan le
Ql
Kadota
Shigetoshi
S
D031202:Chrysanthemum / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
D005419:Flavonoids; D005960:Glucosides; D006027:Glycosides; D039821:Monoterpenes; D010636:Phenols; C477955:acacetin-7-O-glucoside; C472707:caffeoylquinic acid; D011801:Quinic Acid; D014969:Xanthine Oxidase