Activity-based high-throughput profiling of metalloprotease inhibitors using small molecule microarrays.
We herein describe a high-throughput small molecule microarray (SMM) method that enables quick and cost-effective identification of potent inhibitors of metalloproteases in an activity-dependent manner, thereby offering a rapid means for inhibitor discovery and profiling.
Wang
Jun
J
Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
Uttamchandani
Mahesh
M
Sun
Li Ping
LP
Yao
Shao Q
SQ
D004791:Enzyme Inhibitors / Q000494:pharmacology*; D020869:Gene Expression Profiling / Q000191:economics / Q000379:methods*; D045726:Metalloproteases / Q000037:antagonists & inhibitors*; D046228:Microarray Analysis / Q000191:economics / Q000379:methods*; D036103:Nanotechnology / Q000191:economics / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004791:Enzyme Inhibitors; D045726:Metalloproteases