title: Effects of inhaled eotaxin on airway function and inflammatory cell influx in sensitised and non-sensitised guinea pigs.
abstract: Eotaxin is a chemokine that has high potency and selectivity as a chemoattractant agent for eosinophils, signalling exclusively through the CCR3 receptor. Eotaxin is upregulated in the lungs within 3 h of antigen challenge, levels peak at 6 h in lung tissue and bronchoalveolar (BAL) fluid, and fall within 12 h of exposure. This study aimed to look at the effect(s) of eotaxin inhalation on airway function in guinea pigs, to determine if the expected inflammatory cell (eosinophil) infiltration could induce airway hyperreactivity (AHR) and a bronchoconstrictor response equivalent to the late asthmatic response (LAR) seen after antigen challenge. Animals were sensitised with 100 microg/ml OA with a dose on days 1 and 5. Airway responses to inhaled eotaxin (10 or 20 microg/ml) were determined by whole body plethysmography as the change in specific airway conductance (sGaw). Inhaled histamine (1mM) was used to investigate AHR, and cell influx was determined by BAL. Senitised animals exposed to 10 microg/ml eotaxin did not reveal a bronchoconstrictor response or AHR and cellular infiltration to the lungs was not evident 24 h after exposure. Both sensitised and non-sensitised animals exposed to 20 microg/ml eotaxin however revealed a significant bronchoconstrictor response 6h post-challenge, with reductions in sGaw of -27.0+/-6.6% and -32.3+/-6.8%, respectively. Both groups also displayed a bronchoconstrictor response to inhaled histamine 24h after exposure, indicating AHR, and a significant increase in both total and differential cell counts. Sensitised animals, however, revealed a significant increase in cell influx compared to non-sensitised animals. Nebulised eotaxin can reveal a LAR, AHR to inhaled histamine, and cellular infiltration to the lungs, possibly via the mobilisation of eosinophils from the bone marrow, and their subsequent recruitment to the airways.
authors.affiliation: Division of Pharmacology, Welsh School of Pharmacy, Cardiff University, Cathays Park, Cardiff, UK.
authors.forename: Nicola
authors.initials: N
authors.lastname: Smith
authors.forename: Frederick J
authors.initials: FJ
authors.lastname: Johnson
mesh_terms: D000280:Administration, Inhalation; D000403:Airway Resistance / Q000187:drug effects / Q000502:physiology; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000139:chemically induced / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016084:Bronchoconstriction / Q000187:drug effects / Q000502:physiology; D002452:Cell Count; D054413:Chemokine CCL11; D019742:Chemokines, CC / Q000008:administration & dosage / Q000494:pharmacology*; D003627:Data Interpretation, Statistical; D006168:Guinea Pigs; D006632:Histamine; D006967:Hypersensitivity / Q000473:pathology* / Q000503:physiopathology*; D007249:Inflammation / Q000473:pathology*; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D010993:Plethysmography, Whole Body; D012143:Respiratory Physiological Phenomena / Q000187:drug effects*
chemical_list: D054413:Chemokine CCL11; D019742:Chemokines, CC; D006632:Histamine; D010047:Ovalbumin