Survey of inward ionic currents acquired by the cochleovestibular ganglion of the early-aged embryonic chick.
The acquisition of ion channels is critical to the formation of neuronal pathways in the peripheral and central nervous systems. This study describes the different types of inward currents (Ii) recorded from the soma of isolated cochleovestibular ganglion (CVG) cells of the embryonic chicken, Gallus gallus. Cells were isolated for whole-cell tight-seal recording from embryonic day (ED) 3, an age when the CVG is a cell cluster, to ED 9, an age when the cochlear and vestibular ganglia (CG, VG) are distinct structures. Results show Na+ and Ca2+ currents (INa and ICa) are acquired by ED 3, although INa dominates with greater density levels that peak by ED 6-7 in VG neurons. In the CG, INa acquisition is slower, reaching peak values by ED 8-9. Isolation of ICa, using Ba2+ as the charge carrier, showed both transient (IBaT)- and sustained (IBaL)-type currents on ED 3. Unlike INa, IBa density varied with age and ganglion. Total IBa increased steadily, showing a decline only in CG cells on ED 8-9 as a result of a decrease in IBaT. IBaL density increased over time, reaching a maximum on ED 6-7 in VG cells, followed by a decline on ED 8-9. In comparison, IBaL in CG neurons, did not increase significantly beyond mean values measured on ED 5. The early onset of these currents and the variations in Ca2+ channel expression between the ganglia suggests that intracellular signals relevant to phenotypic differentiation begin within these early time frames.
Sokolowski
Bernd H A
BH
Department of Otolaryngology-HNS, University of South Florida, Tampa, Florida 33612, USA. bsokolow@hsc.usf.edu
D000818:Animals; D015220:Calcium Channels / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D003051:Cochlea / Q000196:embryology / Q000378:metabolism / Q000502:physiology*; D004594:Electrophysiology; D017950:Ganglia, Sensory / Q000378:metabolism / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010641:Phenotype; D015222:Sodium Channels / Q000502:physiology; D014722:Vestibule, Labyrinth / Q000196:embryology / Q000378:metabolism / Q000502:physiology*
D015220:Calcium Channels; D007473:Ion Channels; D015222:Sodium Channels