title: Precise characterization of human histones in the H2A gene family by top down mass spectrometry.
abstract: Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were alpha-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding approximately 10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Boyne
authors.forename: James J
authors.initials: JJ
authors.lastname: Pesavento
authors.forename: Craig A
authors.initials: CA
authors.lastname: Mizzen
authors.forename: Neil L
authors.initials: NL
authors.lastname: Kelleher
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry
chemical_list: D006657:Histones; D020033:Protein Isoforms