title: Gene expression profiles of oral leukoplakia and carcinoma: genome-wide comparison analysis using oligonucleotide microarray technology.
abstract: Although leukoplakia is the most common precancerous lesion of the oral cavity, its molecular biological properties are largely unknown. The aim of this study was to identify the genes responsible for its pathogenesis and malignant transformation using oligonucleotide microarray technology. The expression profiles of 8,800 genes in human oral leukoplakia (n=4) and oral squamous cell carcinoma (OSCC) (n=2) were analyzed using the Affymetrix GeneChip system and the results were confirmed with RT-PCR. Eight genes were up-regulated (>2.0-fold) and ten were down-regulated (<0.5-fold) in all leukoplakias analyzed with the GeneChip. In particular, loricrin and keratins displayed greater differences between normal tissue and leukoplakia. Some of the 18 alternatively expressed genes were markedly down-regulated in squamous cell carcinoma compared with leukoplakia. Our data suggested that gene abnormalities in cytoskeleton network components might be responsible for the development and progression of oral leukoplakia.
authors.affiliation: Department of Oral & Maxillofacial Surgery, School of Dentistry, Showa University, Ohta-ku, Tokyo 145-8515, Japan. odani@senzoku.showa-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Odani
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.forename: Ming-Heng
authors.initials: MH
authors.lastname: Li
authors.forename: Ai
authors.initials: A
authors.lastname: Kawamata
authors.forename: Tomohide
authors.initials: T
authors.lastname: Isobe
authors.forename: Masayasu
authors.initials: M
authors.lastname: Iwase
authors.forename: Masao
authors.initials: M
authors.lastname: Nagumo
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D007972:Leukoplakia, Oral / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000235:genetics
chemical_list: D008565:Membrane Proteins; C064554:loricrin