Collagenases in gingival crevicular fluid in type 1 diabetes mellitus.
BACKGROUND
Studies have demonstrated that high levels of collagenase activity in gingival crevicular fluid (GCF) are associated with degradation of periodontal tissues in progressive periodontitis compared to periodontally healthy tissues. Because the activation of collagenases is an important issue in periodontitis, we have studied the activation of collagenase in gingival crevicular fluid samples of diabetic patients.


METHODS
Collagenase activity was studied in human gingival crevicular fluids. Twenty-two poorly controlled diabetic patients (e.g., blood glucose: 11.0+/-0.7 mmol/l; hemoglobin A1c [HbA1c]: 9.6%+/-0.3%) and five well-controlled diabetic patients were compared to six chronic periodontitis subjects and five healthy controls. Collagenase activity against type I collagen was measured using sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis quantitated by laser densitometry.


RESULTS
The poorly controlled diabetic patients had more alveolar bone loss than the well-controlled diabetic subjects and controls (P<0.001; t test). The activity of collagenases in GCF in poorly controlled diabetic patients was similar to that seen in chronic periodontitis subjects (P>0.05) but higher than in healthy controls (P<0.01; t test), whereas there was no difference between the well-controlled diabetic subjects and systemically healthy controls (P>0.05; t test).


CONCLUSIONS
Poorly controlled diabetes is strongly related to periodontal tissue destruction, and collagenases in GCF may mediate and reflect this effect.
Safkan-Seppälä
Bedia
B
Institute of Dentistry, University of Helsinki, and Department of Medicine/Invärtes Medicin, Helsinki University Hospital, Helsinki, Finland.
Sorsa
Timo
T
Tervahartiala
Taina
T
Beklen
Arzu
A
Konttinen
Yrjö T
YT
D000328:Adult; D000368:Aged; D016301:Alveolar Bone Loss / Q000145:classification / Q000201:enzymology; D001786:Blood Glucose / Q000032:analysis; D002908:Chronic Disease; D024042:Collagen Type I / Q000378:metabolism; D017364:Collagenases / Q000032:analysis*; D003720:Densitometry; D003774:Dental Plaque Index; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000145:classification / Q000201:enzymology; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification / Q000201:enzymology; D010518:Periodontitis / Q000145:classification / Q000201:enzymology
D001786:Blood Glucose; D024042:Collagen Type I; D017364:Collagenases; D006442:Glycated Hemoglobin