Patulibacter minatonensis gen. nov., sp. nov., a novel actinobacterium isolated using an agar medium supplemented with superoxide dismutase, and proposal of Patulibacteraceae fam. nov.
A novel Gram-positive bacterial strain, designated KV-614T, was isolated from a soil sample using an agar medium supplemented with superoxide dismutase. Based on 16S rRNA gene sequence analysis, it was found that the strain represented a novel deep-rooting lineage within the class Actinobacteria and clustered with yet-uncultivated bacteria from terrestrial environments and some unidentified strains isolated by unique methods. The most closely related established genus was Conexibacter (92.4% sequence similarity to Conexibacter woesei DSM 14684T). Cells of strain KV-614T were rod-shaped and motile with long flagella. The strain was catalase-positive, oxidase-negative and grew under aerobic conditions. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and alanine and glutamic acid. The peptidoglycan acyl type was acetyl. The only detected isoprenoid quinone was demethylmenaquinone with seven isoprene units (DMK-7). Mycolic acids were not detected. The predominant cellular fatty acid was omega9c-octadecenoic acid (C18:1omega9c). Minor components were 12-methyl tetradecanoic acid (anteiso-C15:0) and 14-methyl hexadecanoic acid (anteiso-C17:0). The DNA G+C content was 72 mol%. On the basis of phenotypic and genotypic characteristics, it is proposed that strain KV-614T represents a new genus and a novel species, Patulibacter minatonensis gen. nov., sp. nov., in the class Actinobacteria. The type strain is KV-614T (=NRRL B-24346T=JCM 12834T=NBRC 100761T). The creation of the family Patulibacteraceae fam. nov. is proposed to encompass the genus Patulibacter gen. nov.
Takahashi
Yōko
Y
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Matsumoto
Atsuko
A
The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
Morisaki
Kurimi
K
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Ōmura
Satoshi
S
The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.|Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000362:Agar; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D001482:Base Composition; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013482:Superoxide Dismutase / Q000378:metabolism*
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D000362:Agar; D013482:Superoxide Dismutase