title: Cryo-EM and single particles.
abstract: Cryoelectronmicroscopy is a method for the imaging of macromolecules in the electron microscope. It was originally developed to determine membrane protein structures from two-dimensional crystals, but more recently "single-particle" techniques have become powerful and popular. Three-dimensional reconstructions are obtained from sets of single-particle images by extensive computer processing; the methods are being applied to many macromolecular assemblies.
authors.affiliation: Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: Liguo
authors.initials: L
authors.lastname: Wang
authors.forename: Fred J
authors.initials: FJ
authors.lastname: Sigworth
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D020285:Cryoelectron Microscopy / Q000379:methods*; D003461:Crystallography / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D046911:Macromolecular Substances; D019837:Ryanodine Receptor Calcium Release Channel / Q000032:analysis / Q000648:ultrastructure
chemical_list: D046911:Macromolecular Substances; D019837:Ryanodine Receptor Calcium Release Channel