Evaluation of the contraceptive potential of brZPCp vaccine in BALB/c mice: their safety and efficacy.
In this study we have examined the potential ability of Microtus branditi partial ZPC (brZPCp) cDNA sequence (436-1150 nt) as a target for immunocontraception. Immunogenicity studies and fertility trials were performed in BALB/c mice using recombinant construction pCR3.1-brZPC(p). ELISA outcome indicated that antibodies could be generated by immunized mice, and IgG titer was increased compared to the control. Immunohistochemistry outcome indicated that antibodies could recognize native ZP in vivo, which in turn, prevented the binding of sperm to ovulated eggs. Antibodies could also recognize recombinant protein expressed by BL21 in vitro, which was confirmed by SDS-PAGE and Western blot. Fertility rate was reduced by 45% compared to the control immunized with pCR3.1. Meanwhile, there was no incidence of significant ovarian pathology in treated mice. This experiment indicates that this vaccine can elicit the specific antibody which binds exactly to the corresponding ZPC. This construction is proved to be immunogenic, and can reduce fertility without obvious oophoritis. The result in this study suggests a potentially important method for controlling population for its safety and easy production.
Xu
Li
L
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Peng
Jing-Pian
JP
Shi
Shu-Qun
SQ
D000818:Animals; D003411:Arvicolinae / Q000235:genetics; D003269:Contraception, Immunologic; D016207:Cytokines / Q000096:biosynthesis; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D004527:Egg Proteins / Q000235:genetics / Q000276:immunology*; D005260:Female; D005298:Fertility; D006367:HeLa Cells; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D022642:Vaccines, Contraceptive / Q000009:adverse effects / Q000276:immunology; D019444:Vaccines, DNA / Q000009:adverse effects / Q000276:immunology; D015044:Zona Pellucida / Q000276:immunology
D016207:Cytokines; D018076:DNA, Complementary; D004527:Egg Proteins; D007074:Immunoglobulin G; D022642:Vaccines, Contraceptive; D019444:Vaccines, DNA