Osmoprotection of Salmonella enterica serovar Typhimurium by Ngamma-acetyldiaminobutyrate, the precursor of the compatible solute ectoine.
N(gamma)-acetyl-2,4-diaminobutyrate (NADA), the precursor of the compatible solute ectoine, was shown to function as an osmoprotectant for the non-halophilic bacterium Salmonella enterica serovar Typhimurium. The addition of NADA-containing extracts of an ectoine synthase mutant of the broad salt-growing halophile Chromohalobacter salexigens DSM 3043(T) could alleviate the inhibitory effects of high salinity in S. enterica, which lacks the ectoine biosynthetic pathway. NADA, purified from extracts of the mutant, protected S. enterica against salinity stress. This osmoprotective effect was slightly lower than that of ectoine, but more potent than that of hydroxyectoine. Accumulation of purified NADA by S. enterica was demonstrated by (13)C-NMR spectroscopy and HPLC analysis. In addition, it was shown that NADA was taken up by S. enterica via the ProP and ProU transport systems, which are known to transport glycine betaine and proline. This finding provides evidence that these permeases can recognize a diaminoacid that carries an unsubstituted alpha-amino group. This is the first time that NADA has been connected with osmoprotective functions in non-halophilic bacteria.
García-Estepa
Raul
R
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain.
Cánovas
David
D
Iglesias-Guerra
Fernando
F
Ventosa
Antonio
A
Csonka
Laszlo N
LN
Nieto
Joaquín J
JJ
Vargas
Carmen
C
D000599:Amino Acids, Diamino / Q000378:metabolism* / Q000494:pharmacology; D000613:Aminobutyrates / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D044129:Halomonadaceae / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response; D006836:Hydro-Lyases / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D009994:Osmolar Concentration; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D012965:Sodium Chloride; D027981:Symporters; D014882:Water-Electrolyte Balance
D000599:Amino Acids, Diamino; D000613:Aminobutyrates; C401954:N(gamma)-acetyl-2,4-diaminobutyric acid; D027981:Symporters; C121557:hydroxyectoine; D012965:Sodium Chloride; C045628:ectoine; D006836:Hydro-Lyases; C106231:ectoine synthase