Electrophoresis of DNA in human genetic diagnostics - state-of-the-art, alternatives and future prospects.
Electrophoretic separation of nucleic acids according to their molecular weights has dominated the methods' spectrum in molecular genetics for nearly half a century. We review the current methodological basis and evaluate its impact with special reference to new developments in the microarray technology. Although electrophoresis may be made redundant for many applications in DNA diagnostics within a few years, a number of electrophoretic vestiges will remain irreplaceable in the foreseeable future.
Gödde
René
R
Department of Human Genetics, Ruhr-University, Bochum, Germany. rene.goedde@rub.de
Akkad
Denis-Amer
DA
Arning
Larissa
L
Dekomien
Gabriele
G
Herchenbach
Julia
J
Kunstmann
Erdmute
E
Meins
Moritz
M
Wieczorek
Stefan
S
Epplen
Jörg T
JT
Hoffjan
Sabine
S
D015152:Blotting, Northern; D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods* / Q000639:trends; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary; D006801:Humans; D046210:Microfluidic Analytical Techniques; D018895:Microsatellite Repeats; D020411:Oligonucleotide Array Sequence Analysis; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
D004247:DNA