Flow injection chemiluminescence immunoassay for 17beta-estradiol using an immunoaffinity column.
A new flow injection chemiluminescent immunoassay was developed for the detection of 17beta-estradiol (E2). The method uses p-iodophenol (PIP) as enhancer and is based on a solid-phase immunoassay format in which an E(2)-OVA immobilized immunoaffinity column inserted in the flow system is used to trap unbound horseradish peroxidase (HRP)-labeled anti-E2 antibody after an off-line incubation of E2 with HRP-labeled anti-E2 antibody. The trapped enzyme conjugate was detected by injecting substrates to produce an enhanced chemiluminescence (CL) response. The linear range for E2 was 10.0-1,000.0 ng mL(-1) with a correlation coefficient of 0.996 and a detection limit of 3.0 ng mL(-1). The sampling and chemiluminescence detection time for one sample was 400 s after a pre-incubation procedure of 30 min. Serum samples detected by this method were in good agreement with the results obtained by EIA with E2-biotin.
Wang
Shuhao
S
College of Environmental Science and Engineering, Donghua University, Shanghai 200051, People's Republic of China.
Lin
Shilei
S
Du
Lingyun
L
Zhuang
Huisheng
H
D002138:Calibration; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D004958:Estradiol / Q000097:blood*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity
D004958:Estradiol