Temporally and spectrally resolved sampling imaging with a specially designed streak camera.
We present a novel sampling imaging technique capable of performing simultaneous two-dimensional measurements of the temporal and spectral characteristics of light-emission processes by use of a specially designed streak camera. A proof-of-principle experiment was performed with a homemade multifocal multiphoton fluorescence microscope. The system was calibrated with a Fabry-Perot etalon and a standard fluorophore solution (rhodamine 6G in ethanol) and was shown to have temporal and spectral resolution of 6.5 ps and 3 nm, respectively, as well as high accuracy and reproducibility in lifetime and spectrum measurement. Temporally and spectrally resolved images of 4 x 4 foci on the sample can be obtained with a snapshot.
Qu
Junle
J
Institute of Optoelectronics, Shenzhen University, China. jlqu@szu.edu.cn
Liu
Lixin
L
Chen
Danni
D
Lin
Ziyang
Z
Xu
Gaixia
G
Guo
Baoping
B
Niu
Hanben
H
D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods