Expression, purification, crystallization and preliminary X-ray analysis of human spindlin1, an ovarian cancer-related protein.
Human spindlin1 is a newly screened and identified gene product related to ovarian carcinomas and is highly homologous to mouse spindlin. It is an abundant maternal transcript expressed in the mouse during the transition from oocyte to embryo. Here, the recombinant human spindlin1 has been overexpressed in Escherichia coli BL21, purified and crystallized using the hanging-drop vapour-diffusion method. Crystals diffracting to 2.25 A resolution were obtained using ammonium sulfate as precipitant. The crystals belong to the space group P2(1)2(1)2(1), with unit-cell parameters a =40.7 A, b =84.4 A, c =136.4 A, alpha=beta=gamma=90 degrees . Assuming two molecules per asymmetric unit, the solvent content is calculated to be 42.4%.
Jiang
Fuguo
F
Laboratory of Structural Biology, Tsinghua University, Beijing, 100084, China.
Zhao
Qiang
Q
Qin
Lipeng
L
Pang
Hai
H
Pei
Xuetao
X
Rao
Zihe
Z
D018797:Cell Cycle Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins
D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D010750:Phosphoproteins; C104434:spindlin