Intraperitoneal stability of alginate-polyornithine microcapsules in rats: an FTIR and SEM analysis.
Alginate-polycation microcapsule systems have been used over decades as delivery vehicles for cell and protein therapy. These systems have been unpredictable across a range of indications with questions resulting around the inherent stability of the alginate polysaccharide and failure mode of the delivery system. The current study focuses on such a system using 5 different alginates, 2 of which are commercially purified, which are crosslinked by polyornithine. Capsules formed by frequency-generated droplet formation were studied in the peritoneal cavity of Long-Evans rats over the course of 3 months by morphometry, Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy of the surface. Individual capsule components were also investigated on FTIR and a relative stability index was generated by titration for comparison to explanted samples over time. Using these techniques, a distinct degradation pattern was noted and is compared between the 5 alginate sources.
LCT BioPharma, Inc., Providence, RI 02906, USA. cgthanos@gmail.com
Christopher G
CG
Thanos
Briannan E
BE
Bintz
William J
WJ
Bell
Haitao
H
Qian
Patricia A
PA
Schneider
Daniel H
DH
MacArthur
Dwaine F
DF
Emerich
D000464:Alginates / Q000737:chemistry / Q000493:pharmacokinetics*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000493:pharmacokinetics*; D002214:Capsules; D016503:Drug Delivery Systems; D004355:Drug Stability; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry / Q000493:pharmacokinetics*; D010529:Peritoneal Cavity; D051381:Rats; D020318:Rats, Long-Evans; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D010455:Peptides; C008973:polyornithine