Experimental study of verapamil on the relaxation of isolated human corpus cavernosum tissues.
OBJECTIVE
To evaluate the relaxant effect of verapamil on human corpus cavernosum in vitro and to assess the drug's potential as a treatment for erectile dysfunction (ED).


METHODS
Preparations of the human corpus cavernosum were obtained from recently deceased young men who had had normal erectile function. The isometric tension and detailed curves were recorded when contractions induced by 10 micromol/L phenylephrine were reduced by different doses of verapamil or the vehicle control (sterile water). The tension of human corpus cavernosum preparations are described as a percentage of their top tension before adding verapamil or the vehicle. ANOVA and least significant difference tests were used for statistical analysis.


RESULTS
Doses of 1 micromol/L, 10 micromol/L and 100 micromol/L verapamil resulted in relaxation of (35.28+/-7.96)%, (55.91+/-6.41)%, (85.68+/-4.16)% after 30 min, respectively. The vehicle control at the same time point produced relaxation of (-0.06+/-10.57)% (P<0.05).


CONCLUSIONS
Verapamil is significantly effective in relaxing normal human corpus cavernous smooth muscle induced by phenylephrine in vitro and the relaxant effect depends on the concentration of verapamil.
Department of Urology, Third Affiliated Hospital, Peking University, Beijing 100083, China.
Lu-Lin
LL
Ma
Yu-Qing
YQ
Liu
Wen-Hao
WH
Tang
Lian-Ming
LM
Zhao
Hui
H
Jiang
D000328:Adult; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000502:physiology; D010413:Penis / Q000187:drug effects / Q000502:physiology; D014700:Verapamil / Q000494:pharmacology*
D014700:Verapamil