The Arabidopsis-mei2-like genes play a role in meiosis and vegetative growth in Arabidopsis.
The Arabidopsis-mei2-Like (AML) genes comprise a five-member gene family related to the mei2 gene, which is a master regulator of meiosis in Schizosaccharomyces pombe and encodes an RNA binding protein. We have analyzed the AML genes to assess their role in plant meiosis and development. All five AML genes were expressed in both vegetative and reproductive tissues. Analysis of AML1-AML5 expression at the cellular level indicated a closely similar expression pattern. In the inflorescence, expression was concentrated in the shoot apical meristem, young buds, and reproductive organ primordia. Within the reproductive organs, strong expression was observed in meiocytes and developing gametes. Functional analysis using RNA interference (RNAi) and combinations of insertion alleles revealed a role for the AML genes in meiosis, with RNAi lines and specific multiple mutant combinations displaying sterility and a range of defects in meiotic chromosome behavior. Defects in seedling growth were also observed at low penetrance. These results indicate that the AML genes play a role in meiosis as well as in vegetative growth and reveal conservation in the genetic mechanisms controlling meiosis in yeast and plants.
Kaur
Jagreet
J
Centre for Cellular and Molecular Biology, Hyderabad 500007, India.
Sebastian
Jose
J
Siddiqi
Imran
I
D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005718:Gametogenesis / Q000502:physiology; D008540:Meiosis / Q000235:genetics*; D018519:Meristem / Q000033:anatomy & histology / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000502:physiology; D016254:Mutagenesis, Insertional; D009698:Nucleoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D010802:Phylogeny; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D012098:Reproduction; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D016415:Sequence Alignment
C108559:AML1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D009698:Nucleoproteins; D012333:RNA, Messenger; D018749:RNA, Plant