Epstein-Barr virus in oral hairy leukoplakia scrapes: identification by PCR.
Oral hairy leukoplakia (OHL) is a lesion associated with a compromised immune system, and its diagnosis is determined by the demonstration of the presence of Epstein-Barr virus (EBV) in lesional tissue. The purpose of this article was to develop a simple technique to help the diagnosis of OHL, using PCR as an alternative technique to evidence EBV in scrapings. DNA samples were obtained by scraping the lateral border of the tongue of 38 adult patients: 29 HIV-positive patients (4 with clinical evidence of OHL; 4 with history of OHL, but without lesion at the moment the samples were collected; and 21 without clinical evidence of OHL), and 9 healthy volunteers for the control group. DNA was extracted from scrapes and amplified by PCR using specific primers for EBV. Of the 29 cases of HIV-positive patients, 22 (75.86%) were positive for EBV: 2 patients with clinical evidence of OHL, 4 patients with history of OHL, but without lesion at the moment the samples were collected, and 16 patients without clinical evidence of OHL. In the control group, samples of 5 (55.56%) healthy volunteers presented amplification for EBV. We concluded that the use of PCR in oral scrapes suggests a high sensitivity but low specificity for the diagnosis of OHL.
Department of Pathology, Health Science Center, Federal University of Santa Catarina.
Tatiana Lucianelli
TL
Komatsu
Elena Riet Correa
ER
Rivero
Marina Helena Cury
MH
Gallottini de Magalhães
Fabio Daumas
FD
Nunes
D000328:Adult; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D015658:HIV Infections / Q000150:complications*; D006679:HIV Seropositivity / Q000821:virology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D017733:Leukoplakia, Hairy / Q000175:diagnosis / Q000821:virology*; D009061:Mouth Mucosa / Q000821:virology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014059:Tongue / Q000821:virology
D004279:DNA, Viral