Edaravone diminishes free radicals from circulating neutrophils in patients with ischemic brain attack.
OBJECTIVE
Treatment with a free radical scavenger could be a new option for ischemic brain attack, however, little is known about the alteration of oxidative stress markers induced by edaravone, a novel free radical scavenger, in human ischemic brain attack.


METHODS
We investigated the effects of edaravone on the oxidative stress markers in patients with ischemic brain attack. Twenty-one patients with ischemic brain attack and 19 controls were enrolled in this study. Blood samples were obtained just before and soon after the first administration of edaravone (30 mg) or ozagrel (40 mg). Intracellular reactive oxygen species of neutrophils were measured using 6-carboxy-2', 7'-dichlorodihydrofluorescin diacetate and a fluorescence-activated cell sorter. Superoxide from neutrophils, induced by phorbol myristate acetate (PMA), was determined by luminol-amplified chemiluminescence assay.


RESULTS
Treatment with 30 mg of edaravone significantly decreased the intracellular reactive oxygen species (ROS) of neutrophils (Wilcoxon test, p=0.0001) and PMA-induced superoxide produced by neutrophils (Wilcoxon test, p=0.001). Ozagrel did not alter the intracellular ROS or superoxide production of neutrophils.


CONCLUSIONS
Reduction of intracellular ROS and suppression of superoxide production in neutrophils provide a potential explanation for the clinical efficacy of edaravone in patients with ischemic brain attack.
Aizawa
Hitoshi
H
The First Department of Medicine, Asahikawa Medical College, Asahikawa
Makita
Yoshihiro
Y
Sumitomo
Kazuhiro
K
Aburakawa
Yoko
Y
Katayama
Takayuki
T
Nakatani-Enomoto
Setsu
S
Suzuki
Yasuhiro
Y
Fujiwara
Kazuhiko
K
Enomoto
Hiroyuki
H
Kuroda
Kenji
K
Kimura
Takashi
T
Yahara
Osamu
O
Koyama
Satoshi
S
Maruyama
Junichi
J
Nakamura
Masao
M
Hasebe
Naoyuki
N
Kikuchi
Kenjiro
K
D000368:Aged; D000369:Aged, 80 and over; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D002545:Brain Ischemia / Q000097:blood* / Q000188:drug therapy*; D000077553:Edaravone; D005260:Female; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D005609:Free Radicals / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*
D016166:Free Radical Scavengers; D005609:Free Radicals; D000077553:Edaravone; D000983:Antipyrine