Competitive study of monoclonal antibodies against the HIV-1 Gp41 core structure.
Monoclonal antibodies (MAbs) 50.69, 98.6, and T26 bind specifically to the core structure of the human immunodeficiency virus type 1 (HIV-1) envelope transmembrane glycoprotein (gp41). To clarify the specificity of the anti-core structure MAbs, we performed competitive assays using the MAbs to the H9 human T cell line infected with the IIIB strain of HIV-1 (H9/IIIB). Bound MAb 50.69 inhibited MAb 98.6 binding unidirectionally. The reason for the unidirectional cross competition between MAbs 50.69 and 98.6 is not clear, but these results help to define the antigenic structure of gp41 on the surface of infected cells.
Division of Infectious and Respiratory Diseases, Internal Medicine, Graduate School of Medicine, Tohoku University, Japan.
Osamu
O
Usami
Peng
P
Xiao
Hong
H
Ling
Toshio
T
Hattori
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D001667:Binding, Competitive; D002460:Cell Line; D000939:Epitopes / Q000276:immunology; D015700:HIV Envelope Protein gp41 / Q000276:immunology* / Q000378:metabolism; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015700:HIV Envelope Protein gp41