Characterization of a human Bid homologue protein from Gallus gallus.
Bid protein, a member of the "BH3-only" subgroup of Bcl-2 family, plays a critical role in mammalian apoptosis regulation. In this study, we have cloned the chicken Bid gene, which encodes a 193 amino acid protein and shares 40% homology with human and mouse Bid proteins. Bid sequence comparison emphasises the conservation of both the functional domain BH3 and the proteolytic cleavage sites. An induction of apoptosis by chicken Bid and the cleavage of the protein, after TNFalpha treatment, were also demonstrated. In addition, mRNA Bid expression was detected along all embryo stages and tissues examined, suggesting a role for this protein in the developmental process. This is the first report demonstrating the functionality of a "BH3-only" protein in chicken.
Díaz-Gil
Gema
G
Departamento de Ciencias de la Salud III, Universidad Rey Juan Carlos, 28922, Alcorcón, Madrid, Spain. gema.diaz@urjc.es
Gómez-Esquer
Francisco
F
Agudo
David
D
Delcán
José
J
Martínez-Arribas
Fernando
F
Rivas
Carmen
C
Schneider
José
J
Palomar
Maria Angustias
MA
Linares
Rafael
R
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051023:BH3 Interacting Domain Death Agonist Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002642:Chick Embryo; D002645:Chickens; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D051023:BH3 Interacting Domain Death Agonist Protein; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha