Cell-mediated response at the muscle phase of Trichinella pseudospiralis and Trichinella spiralis infections.
The cell-mediated response in BALB/c mice infected either by Trichinella pseudospiralis or Trichinella spiralis was compared at days 30-50 post-infection (muscle phase). The former species is non-encapsulated, whereas the latter is encapsulated in host muscles. The pattern of response against the two species was similar. Both species elicited T(H)0 or T(H)1/T(H)2 response, with the last one being dominant. Productions of interferon gamma (IFN-gamma), interleukin (IL)-4 and IL-5 were observed after antigenic restimulation of splenocytes from infected mice. No significant difference was observed between the levels of response to concanavalin A (Con-A) by the splenocytes from both infected and non-infected animals. There was a significant increase in serum IgG(1) and IgG(2a). Flow cytometric analysis revealed a marked proliferative response of splenocytes from infected mice to worm antigens, dominated by B (CD19) lymphoblasts. Only a few helper (CD4+) and cytotoxic (CD8+) T lymphoblasts were present. This was confirmed by an up-regulation of CD69, with a dominant expression on B lymphoblasts. In conclusion, the minimal or lack of intense cellular response against T. pseudospiralis in muscles is likely not due to depression of cell-mediated immunity.
Lee
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KM
5S-13, Kadoorie Biological Sciences Building, Department of Zoology, The University of Hong Kong, Hong Kong, SAR, PR China.
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D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D018941:Antigens, CD19 / Q000032:analysis; D016827:CD8 Antigens; D002478:Cells, Cultured; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D007814:Larva / Q000254:growth & development; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000469:parasitology; D013154:Spleen / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer; D014234:Trichinella / Q000276:immunology / Q000502:physiology; D017160:Trichinella spiralis / Q000276:immunology / Q000502:physiology; D014235:Trichinellosis / Q000276:immunology* / Q000469:parasitology
D000909:Antibodies, Helminth; D018941:Antigens, CD19; D016827:CD8 Antigens; D007074:Immunoglobulin G; D015848:Interleukin-5; D015847:Interleukin-4; D007371:Interferon-gamma