Preparation of nano-sized particles from collagen II by a high-voltage electrostatic field system.
The pilot study describes a novel method for preparing nano-sized particles from collagen II using a high-voltage electrostatic field system. Observations from transmission electron microscopy showed that, in one of the cases, the nano-sized collagen II particles exhibited good sphericity, and the particles were in the range of 23.3+/-1.7 nm in diameter at the experimental setting of 3 kV cm(-1), for a 3 h treatment period and at 25 degrees C (with a collagen concentration of 0.2 mg ml(-1)). When the treatment temperature increased to 30 degrees C, the collagen II began to lose the tendency to form individually separated spherically shaped nano-particles. Moreover, a fibrous structure of collagen II was formed instead of a nano-particle shape at the temperature of 37 degrees C. This result is probably contributed to by an entropy-driven process that is termed fibrillogenesis, a larger force causing the collagen molecules to self-assemble and then form collagen fibrils. It is interesting to note that this is practically the first attempt to produce nano-particles directly from collagen II solution under the treatment of a high-voltage electrostatic field, together with a set of working parameters for the collagen concentration and low-temperature setting.
Chang
S J
SJ
I-SHOU University, Department of Biomedical Engineering, Kaohsiung County, Taiwan.
Niu
G C-C
GC
Kuo
S M
SM
Ho
C C
CC
Bair
M S
MS
D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000528:radiation effects; D024043:Collagen Type II / Q000138:chemical synthesis* / Q000528:radiation effects / Q000648:ultrastructure*; D003460:Crystallization / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004574:Electromagnetic Fields; D008422:Materials Testing; D046912:Multiprotein Complexes / Q000138:chemical synthesis / Q000528:radiation effects / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure*; D010316:Particle Size; D055672:Static Electricity
D001672:Biocompatible Materials; D024043:Collagen Type II; D046912:Multiprotein Complexes