Rare haplotypes in mtDna: applications in the analysis of biosocial aspects of past human populations.
We report on the use of rare mutations to tackle biosocial questions such as kinship and differential burial practices from past human populations. To do this, we have inferred nucleotide position 73 of HVS-II in individuals classified as belonging to haplogroup H from 76 human dental samples from the necropolis of Aldaieta (Basque Country, Spain, 6th-7th century) by means of PCR and restriction enzyme tests. The same analysis has been performed for 146 extant individuals from the northern Iberian peninsula. A combination of haplotype H and 73G in HVS-II, rare in extant populations (0.5-3%), has been found at a frequency of 20% in the ancient population of Aldaieta. These data can be explained in terms of the existence of different burial practices associated with a variety of factors, mainly social status and kinship. This hypothesis is also supported by archeological data. These results indicate that caution should be taken when making phylogenetic inferences from extinct populations, because an uncharacterized kinship can significantly bias allele frequencies.
Izagirre
Neskuts
N
Department of Genetics, Physical Anthropology, and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), PO Box 644, Bilbao 48080, Spain.
Alzualde
Ainhoa
A
Alonso
Santos
S
Paz
Leire
L
Alonso
Antonio
A
de la Rúa
Concepción
C
D001842:Bone and Bones / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes / Q000235:genetics*; D049690:History, Ancient; D006801:Humans; D008297:Male; D009154:Mutation / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013030:Spain; D014070:Tooth / Q000378:metabolism
D004272:DNA, Mitochondrial