[Experimental study about the protective effect of Ginkgo biloba extract on renal acute ischemia-reperfusion injury in rats].
OBJECTIVE
To study the protective effect of Ginkgo biloba extract(EGb) on the kidney in the case of ischemia-reperfusion injury.


METHODS
The model of renal ischemia-reperfusion injury in male rats was made by ligation of left renal artery for 45 min and 4 h of reperfusion. The rats with pretreament were fed with EGb prior to operation. The change of MDA, SOD, Na+-K+-ATPase, Ca2+-ATPase in kidney and BUN, Cr in plasma were determined. The renal pathologic changes were observed.


RESULTS
After ischemia-reperfusion, the content of MDA in renal cortex and the levels of BUN, Cr in plasma were increased,the activity of SOD,Na+-K+-ATPase, Ca2+-ATPase in renal cortex were decreased, and the phathological changes induced by ischemia-reperfusion in renal tissues were observed clearly. The pretreatment of rats with EGb coued significantly prevente the reduction of SOD, Na+-K+-ATPase, Ca2+-ATPase activity in renal cortex and the increase of MDA content in renal cortex, decrease the concenration of BUN, Cr in plasma. The pathological changes of proximal tubular cells in rats kidneys induced by ischemia-reperfusion were also prevented by the pretreatment with EGb.


CONCLUSIONS
EGb can protect rats from renal injuries caused by ischemia-reperfusion.
Zhao
Run-ying
RY
Dept of Pharmacology, Shenyang Medical College, China. zhaorunying@sohu.com
Li
Yan-hua
YH
Gao
Wen
W
Wang
Yu-xia
YX
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry; D007668:Kidney / Q000473:pathology; D007672:Kidney Cortex / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D008315:Malondialdehyde; D003404:Creatinine; D013482:Superoxide Dismutase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase