Intraluminal injection of short chain fatty acids diminishes intestinal mucosa injury in experimental ischemia-reperfusion.
OBJECTIVE
Investigated the effect of intraluminal short-chain fatty acids (SCFA) on the intestinal mucosa in the presence of ischemia-reperfusion injury (IRI).


METHODS
Six blind sacs of the small bowel (3 at the jejunum and 3 at the ileum) were created in ten Wistar rats. The lateral sacs of both bowel regions were subjected to IRI (15/15 minutes) while the medial sacs were let free to receive blood supply. In the lateral sacs, it was injected either a solution containing SCFA (butyrate, propionate and acetate) or pure saline at the bowel lumen. No fluid was injected in the medial sacs.


RESULTS
Both at the jejunum and at the ileum the score of the mucosal injury was higher in saline than in control sacs. SCFA treated sacs showed lesser score at the ileum (p = 0.03) but were not significantly different at the jejunum (p = 0.83) when compared with saline sacs. It was found a significant greater number of neutrophils (p < 0.01) in the sacs treated with saline than in the other two sacs in both regions.


CONCLUSIONS
SCFA protect the distal small bowel mucosa and diminishes infiltration of neutrophils to the gut lamina propria in IRI.
Aguilar-Nascimento
José Eduardo de
JE
Department of Surgery, Federal University of Mato Grosso, Brazil. aguilar@cpd.ufmt.br
Salomão
Alberto Bicudo
AB
Nochi
Rubens Jardim
RJ
Nascimento
Mariana
M
Neves
José de Souza
Jde S
D000818:Animals; D004195:Disease Models, Animal; D005232:Fatty Acids, Volatile / Q000627:therapeutic use*; D007082:Ileum / Q000098:blood supply / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000293:injuries; D007583:Jejunum / Q000098:blood supply / Q000187:drug effects*; D007958:Leukocyte Count; D009504:Neutrophils / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy*
D005232:Fatty Acids, Volatile