Functional and structural characterization of rhodopsin oligomers.
A major question in G protein-coupled receptor signaling concerns the quaternary structure required for signal transduction. Do these transmembrane receptors function as monomers, dimers, or larger oligomers? We have investigated the oligomeric state of the model G protein-coupled receptor rhodopsin (Rho), which absorbs light and initiates a phototransduction-signaling cascade that forms the basis of vision. In this study, different forms of Rho were isolated using gel filtration techniques in mild detergents, including n-dodecyl-beta-D-maltoside, n-tetradecyl-beta-D-maltoside, and n-hexadecyl-beta-D-maltoside. The quaternary structure of isolated Rho was determined by transmission electron microscopy, demonstrating that in micelles containing n-dodecyl-beta-D-maltoside, Rho exists as a mixture of monomers and dimers whereas in n-tetradecyl-beta-D-maltoside and n-hexadecyl-beta-D-maltoside Rho forms higher ordered structures. Especially in n-hexadecyl-beta-D-maltoside, most of the particles are present in tightly packed rows of dimers. The oligomerization of Rho seems to be important for interaction with its cognate G protein, transducin. Although the activated Rho (Meta II) monomer or dimers are capable of activating the G protein, transducin, the activation process is much faster when Rho exists as organized dimers. Our studies provide direct comparisons between signaling properties of Meta II in different quaternary complexes.
Department of Pharmacology, Case School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Beata
B
Jastrzebska
Dimitrios
D
Fotiadis
Geeng-Fu
GF
Jang
Ronald E
RE
Stenkamp
Andreas
A
Engel
Krzysztof
K
Palczewski
D000818:Animals; D002417:Cattle; D019281:Dimerization; D008027:Light; D008823:Micelles; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D012243:Rhodopsin / Q000737:chemistry / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000378:metabolism*
D008823:Micelles; D012243:Rhodopsin