Redox cycling of adrenaline and adrenochrome catalysed by mitochondrial Complex I.
Complex I in bovine heart submitochondrial particles catalyses the NADH-supported generation of superoxide anion; adrenaline is oxidised by superoxide to adrenochrome that, on its hand, is reduced by Complex I, thus establishing a redox cycle that amplifies the superoxide production. The routes in Complex I for superoxide formation and for adrenochrome reduction appear to be different, since they have a different sensitivity to Complex I inhibitors. The results are discussed in terms of current assays for superoxide detection and of pathologies linked to catecholamine oxidation.
Genova
Maria Luisa
ML
Dipartimento di Biochimica "G. Moruzzi," Università di Bologna, Via Irnerio 48, 40126 Bologna, Italy. marialuisa.genova@unibo.it
Abd-Elsalam
Nagwa M
NM
Mahdy
El Sayed M E
el SM
Bernacchia
Andrea
A
Lucarini
Marco
M
Pedulli
Gian Franco
GF
Lenaz
Giorgio
G
D000323:Adrenochrome / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D002417:Cattle; D002478:Cells, Cultured; D042967:Electron Transport Complex I / Q000378:metabolism*; D004837:Epinephrine / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides; D000323:Adrenochrome; D042967:Electron Transport Complex I; D004837:Epinephrine