Karyotypic characterization of Iheringichthys labrosus (Pisces, Pimelodidae): C-, G- and restriction endonuclease banding.
Various chromosomal banding techniques were utilized on the catfish, Iheringichthys labrosus, taken from the Capivara Reservoir. C-banding regions were evidenced in telomeric regions of most of the chromosomes. The B microchromosome appeared totally heterochromatic. The restriction endonuclease AluI produced a banding pattern similar to C-banding in some chromosomes; the B microchromosome, when present, was not digested by this enzyme and remained stained. G-banding was conspicuous in almost all the chromosomes, with the centromeres showing negative G-banding. When the restriction endonuclease BamHI was used, most of the telomeres remained intact, while some centromeres were weakly digested. The B chromosome was also not digested by this enzyme. The first pair of chromosomes showed a pattern of longitudinal bands, both with G-banding and BamHI; this was more evident with G-banding. This banding pattern can be considered a chromosomal marker for this population of I. labrosus.
de Carvalho
Rafael Augusto
RA
Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brasil.
Dias
Ana Lúcia
AL
D000818:Animals; D002397:Catfishes / Q000235:genetics*; D002871:Chromosome Banding / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D007621:Karyotyping / Q000379:methods / Q000662:veterinary*; D008297:Male
D005819:Genetic Markers; D004262:DNA Restriction Enzymes