gamma-Parvin is dispensable for hematopoiesis, leukocyte trafficking, and T-cell-dependent antibody response.
Integrins regulate cell behavior through the assembly of multiprotein complexes at the site of cell adhesion. Parvins are components of such a multiprotein complex. They consist of three members (alpha-, beta-, and gamma-parvin), form a functional complex with integrin-linked kinase (ILK) and PINCH, and link integrins to the actin cytoskeleton. Whereas alpha- and beta-parvins are widely expressed, gamma-parvin has been reported to be expressed in hematopoietic organs. In the present study, we report the expression pattern of the parvins in hematopoietic cells and the phenotypic analysis of gamma-parvin-deficient mice. Whereas alpha-parvin is not expressed in hematopoietic cells, beta-parvin is only found in myeloid cells and gamma-parvin is present in both cells of the myeloid and lymphoid lineages, where it binds ILK. Surprisingly, loss of gamma-parvin expression had no effect on blood cell differentiation, proliferation, and survival and no consequence for the T-cell-dependent antibody response and lymphocyte and dendritic cell migration. These data indicate that despite the high expression of gamma-parvin in hematopoietic cells it must play a more subtle role for blood cell homeostasis.
Department of Molecular Medicine, Max Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
Haiyan
H
Chu
Ingo
I
Thievessen
Michael
M
Sixt
Tim
T
Lämmermann
Ari
A
Waisman
Attila
A
Braun
Angelika A
AA
Noegel
Reinhard
R
Fässler
D000185:Actinin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement / Q000502:physiology*; D003713:Dendritic Cells / Q000502:physiology; D005298:Fertility / Q000235:genetics; D006410:Hematopoiesis / Q000502:physiology*; D007962:Leukocytes / Q000166:cytology* / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology; D008264:Macrophages / Q000166:cytology / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008840:Microfilament Proteins; D012016:Reference Values; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation; D000096985:Calponins
D000185:Actinin; D008840:Microfilament Proteins; C486590:PARVA protein, human; D000096985:Calponins; C486591:PARVB protein, human; C486593:PARVG protein, human