Assay and functional properties of Rabkinesin-6/Rab6-KIFL/MKlp2 in cytokinesis.
Here we describe methods for the characterization of the kinesin-9 family motor protein Rabkinesin-6/Rab6-KIFL/MKlp2 in cytokinesis. Here we outline biochemical assays for studying the interaction of Rabkinesin-6/Rab6-KIFL/MKlp2 with microtubules, and its regulation and interaction with the mitotic polo-like kinase 1 using recombinant proteins expressed in and purified from insect cells. Protocols for the in vivo functional analysis of Rabkinesin-6/Rab6-KIFL/MKlp2 using depletion with small interfering RNA duplexes are described.
Neef
Rüdiger
R
Grüneberg
Ulrike
U
Barr
Francis A
FA
D000818:Animals; D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology*; D006801:Humans; D016547:Kinesins / Q000502:physiology*; D008024:Ligands; D008870:Microtubules / Q000378:metabolism; D010766:Phosphorylation; D034622:RNA Interference; D011994:Recombinant Proteins / Q000378:metabolism; D018411:Spodoptera
C410089:KIF20A protein, human; D008024:Ligands; D011994:Recombinant Proteins; D016547:Kinesins