Leishmania donovani cyclin 1 (LdCyc1) forms a complex with cell cycle kinase subunit CRK3 (LdCRK3) and is possibly involved in S-phase-related activities.
Expression of Leishmania donovani cyclin 1 (LdCyc1) mRNA during the cell cycle of promastigotes is S-phase specific. Here, we show that the LdCyc1 protein is periodically expressed and the activity of its associated kinase varies during the cell cycle in line with its expression pattern. In addition, we have shown that LdCRK3, homologous to CRK3 from L. mexicana, is the cognate Cdk partner of LdCyc1 and that the activity of the complex is inhibited specifically by heat stable factor(s) from the parasite.
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, India.
Sampali
S
Banerjee
Abhik
A
Sen
Pradeep
P
Das
Partha
P
Saha
D000818:Animals; D016203:CDC2 Protein Kinase / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D019925:Cyclin A / Q000378:metabolism; D019927:Cyclin E / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism* / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007893:Leishmania donovani / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D011494:Protein Kinases / Q000032:analysis; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D016196:S Phase / Q000502:physiology*; D044786:S-Phase Kinase-Associated Proteins / Q000502:physiology*
D019925:Cyclin A; D019927:Cyclin E; D016213:Cyclins; D015800:Protozoan Proteins; D044786:S-Phase Kinase-Associated Proteins; C402346:cyc1 protein, Paramecium tetraurelia; D011494:Protein Kinases; C102333:histone H1 kinase; D016203:CDC2 Protein Kinase