Lineage involvement in chronic myeloid leukaemia: comparison between MBCR/ABL and mBCR/ABL cases.
The relationship between different Abelson/breakpoint cluster region (BCR/ABL+) gene rearrangements and the involvement of different haematopoietic cell lineages were investigated in 15 chronic myeloid leukaemia patients. Analysis of purified cell populations confirmed the involvement of the neutrophil (89%), monocytic (89%), eosinophil (88%), erythroid (100%), and CD34(+) cells (100%) in virtually all patients, without differences between minor BCR/ABL+ and major BCR/ABL+ cases; BCR/ABL+ B- and natural killer (NK)-cells were detected in 43% and 31% of cases, respectively, whereas BCR/ABL+ T-cells were rare (7%). All three minor BCR/ABL+ patients showed involvement of both B- and NK-cells, which was infrequent (27%, P = 0.06 and 10%, P = 0.01) among major BCR/ABL+ cases.
Servicio General de Citometría, Hospital Universitario de Salamanca, Salamanca, Spain.
Daniel
D
Primo
Maria Luz
ML
Sanchez
Ana Belén
AB
Espinosa
María Dolores
MD
Tabernero
Ana
A
Rasillo
Jose M
JM
Sayagués
Marcos
M
Gonzalez
Jose M
JM
Hernandez
Alberto
A
Orfao
D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000235:genetics; D001402:B-Lymphocytes / Q000502:physiology; D001773:Blood Cells / Q000502:physiology; D001854:Bone Marrow Cells / Q000502:physiology; D004804:Eosinophils / Q000502:physiology; D041905:Erythroid Cells / Q000166:cytology; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D015321:Gene Rearrangement / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007694:Killer Cells, Natural / Q000502:physiology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology; D009504:Neutrophils / Q000502:physiology; D010677:Philadelphia Chromosome; D013601:T-Lymphocytes / Q000502:physiology; D014158:Transcription, Genetic / Q000235:genetics
D018952:Antigens, CD34