No age-related change in circulating endothelial progenitor cells in healthy subjects.
Endothelial progenitor cells were isolated from peripheral blood obtained from 32 healthy volunteers without cardiovascular risk factors who ranged in age from 20 to 61 years (mean [+/- SD] age, 34.1 +/- 9.6 years). The fractions of CD34(+) endothelial progenitor cells expressing kinase insert domain receptor-1, CD62E, or CD31 were analyzed with flow cytometry. Correlation analysis demonstrated that there was no significant correlation between subject age and the fraction of circulating CD34(+) mononuclear cells expressing kinase insert domain receptor-1 (P = 0.324; r = -0.180). Similarly, there was no significant correlation between subject age and the fraction of circulating CD34(+) mononuclear cells expressing CD62E (P = 0.496; r = -0.125) or the fraction of circulating CD34(+) mononuclear cells expressing CD31 (P = 0.245; r = -0.212). In conclusion, the experimental results showed that there was no age-related change in the basal level of circulating endothelial progenitor cells in healthy subjects without cardiovascular risk factors.
Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital Kaohsiung, Kaohsiung, Taiwan, ROC.
Mien-Cheng
MC
Chen
Hon-Kan
HK
Yip
Chien-Jen
CJ
Chen
Cheng-Hsu
CH
Yang
Chiung-Jen
CJ
Wu
Cheng-I
CI
Cheng
Yen-Hsun
YH
Chen
Han-Tan
HT
Chai
Ching-Ping
CP
Lee
Hsueh-Wen
HW
Chang
D000328:Adult; D000367:Age Factors; D018952:Antigens, CD34 / Q000097:blood; D019040:E-Selectin / Q000097:blood; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood; D012307:Risk Factors; D013234:Stem Cells / Q000166:cytology*
D018952:Antigens, CD34; D019040:E-Selectin; D019408:Platelet Endothelial Cell Adhesion Molecule-1