Effects of polyvinyl chloride overwrap film, high-oxygen modified atmosphere packaging, or ultra-low-oxygen modified atmosphere packaging on bone marrow discoloration in beef humerus, rib, thoracic vertebra, and scapula.
Meat retailers have reported bone marrow discoloration to be a problem, especially in modified atmosphere packages (MAP). Therefore, it is important to determine the prevalence and cause(s) of bone marrow discoloration in different beef bones and packaging systems. Thirty-six beef humeri, ribs, scapulas, and thoracic vertebrae from USDA Select and Choice carcasses were obtained from a commercial abattoir, cut into 2.54-cm-thick sections at 4 d postmortem, and packaged into 1 of 3 systems: 1) polyvinyl chloride film (PVC) overwrap; 2) high-oxygen (80% O2, 20% CO2) MAP; and 3) ultra-low-oxygen (70% N2, 30% CO2) MAP. Instrumental reflectance and visual color scores were taken on d 0, 2, and 4, and on d 0 to 4 of display, respectively. Bone marrow was extracted from humeri, ribs, and thoracic vertebrae for analysis but not from scapulas. Ribs, scapulas, and thoracic vertebrae packaged in PVC and high-oxygen MAP developed undesirable gray or black discoloration. In ultra-low-oxygen MAP, mean visual color scores were acceptable throughout the entire display period. Discoloration (darkening) was more extensive for ribs, scapulas, and thoracic vertebrae than for humeri, especially for bones packaged in PVC and high-oxygen MAP. Humeri had lower (P < 0.05) a* values (larger positive a* values indicate a redder color) than the other bones. The a* values for ribs, scapulas, and thoracic vertebrae decreased (P < 0.05) over time. Chroma showed that bone marrow discolored during display, but graying was dramatically less for all bones packaged in ultra-low-oxygen MAP and for humeri in PVC and high-oxygen MAP. Humeri marrow had lower (P < 0.05) 2-thiobarbituric acid reactive substances (TBARS) than did ribs and thoracic vertebrae marrow. Ultra-low-oxygen MAP resulted in the least amount of change in TBARS from d 0 to 4, whereas thoracic vertebrae marrow had greater (P < 0.05) TBARS values at d 4 of display than at d 0 in PVC and high-oxygen MAP. Humeri marrow had dramatically less total Fe and hemoglobin than did that of ribs and thoracic vertebrae for all packaging systems. Myoglobin was undetectable in humeri marrow. The much larger amounts of Fe and hemoglobin in ribs and thoracic vertebrae likely contribute to marrow discoloration. Bone marrow discoloration was distinct in ribs, scapulas, and thoracic vertebrae packaged in PVC or high-oxygen MAP. Bones packaged in ultra-low-oxygen MAP had minimal discoloration.
Department of Animal Sciences and Industry, Kansas State University, Manhattan, 66506, USA.
J P
JP
Grobbel
M E
ME
Dikeman
J S
JS
Smith
D H
DH
Kropf
G A
GA
Milliken
D000818:Animals; D001853:Bone Marrow / Q000737:chemistry* / Q000187:drug effects; D002417:Cattle; D018857:Food Packaging / Q000379:methods* / Q000592:standards; D006454:Hemoglobins / Q000032:analysis; D006811:Humerus; D007501:Iron / Q000032:analysis; D016018:Least-Squares Analysis; D008460:Meat / Q000592:standards*; D009211:Myoglobin / Q000032:analysis; D010100:Oxygen / Q000494:pharmacology*; D010758:Phosphorus / Q000032:analysis; D010858:Pigmentation / Q000187:drug effects / Q000502:physiology; D011143:Polyvinyl Chloride / Q000592:standards; D012272:Ribs; D012540:Scapula; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013904:Thoracic Vertebrae; D013997:Time Factors
D006454:Hemoglobins; D009211:Myoglobin; D017392:Thiobarbituric Acid Reactive Substances; D010758:Phosphorus; D011143:Polyvinyl Chloride; D007501:Iron; D010100:Oxygen