Culture medium modulates the behaviour of human dental pulp-derived cells: technical note.
In vitro approaches have extensively been developed to study reparative dentinogenesis. While dental pulp is a source of unidentified progenitors able to differentiate into odontoblast-like cells, we investigated the effect of two media; MEM (1.8 mM Ca and 1 mM Pi) and RPMI 1640 (0.8 mM Ca and 5 mM Pi) on the behaviour of human dental pulp cells. Our data indicate that MEM significantly increased cell proliferation and markedly enhanced the proportion of alpha-smooth muscle actin positive cells, which represent a putative source of progenitors able to give rise to odontoblast-like cells. In addition, MEM strongly stimulated alkaline phosphatase activity and was found to induce expression of transcripts encoding dentin sialophosphoprotein, an odontoblastic marker, without affecting that of parathyroid hormone/parathyroid hormone related protein-receptor and osteonectin. In conclusion, these observations demonstrate that not only proliferation but also differentiation into odontoblast-like cells was induced by rich calcium and poor phosphate medium (MEM) as compared to RPMI 1640. This study provides important data for the determination of the optimal culture conditions allowing odontoblast-like differentiation in human pulp cell culture.
INSERM EM 9903, Research Center on Materials with Biological Interest, School of Dental Surgery, 1 place Alexis Ricordeau, 44042 Nantes cedex 1, France.
S
S
Lopez-Cazaux
G
G
Bluteau
D
D
Magne
B
B
Lieubeau
J
J
Guicheux
B
B
Alliot-Licht
D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects*; D016326:Extracellular Matrix Proteins; D006801:Humans; D009804:Odontoblasts / Q000166:cytology / Q000187:drug effects; D015676:Osteonectin / Q000235:genetics; D010750:Phosphoproteins; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044168:Receptor, Parathyroid Hormone, Type 1 / Q000235:genetics; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein
D000469:Alkaline Phosphatase; D003470:Culture Media; D016326:Extracellular Matrix Proteins; D015676:Osteonectin; D010750:Phosphoproteins; D011498:Protein Precursors; D012333:RNA, Messenger; D044168:Receptor, Parathyroid Hormone, Type 1; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein