Identification of agrinSN isoform and muscle-specific receptor tyrosine kinase (MuSK) [corrected] in sperm.
We previously demonstrated several nicotinic acetylcholine receptor (nAChR) subunits and associated proteins in human sperm. Here, we identified in sperm for the first time two additional nAChR-associated molecules: (1) agrin(SN)Z(+) in human sperm localized in the posterior post-acrosomal, neck, and flagellar mid-piece regions; (2) a low-molecular weight isoform of muscle-specific receptor tyrosine kinase in human and mouse sperm localized in the flagellar mid-piece of human sperm.
Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, CA 95616, USA. pkumar@ucdavis.edu
Priyadarsini
P
Kumar
Michael J
MJ
Ferns
Stanley
S
Meizel
D000177:Acrosome / Q000201:enzymology / Q000378:metabolism; D018171:Agrin / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D020033:Protein Isoforms / Q000302:isolation & purification / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000302:isolation & purification / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000302:isolation & purification / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013082:Sperm Tail / Q000201:enzymology / Q000378:metabolism; D013094:Spermatozoa / Q000201:enzymology / Q000378:metabolism*; D013737:Testis / Q000201:enzymology / Q000378:metabolism
D018171:Agrin; D020033:Protein Isoforms; D011950:Receptors, Cholinergic; C095714:MUSK protein, human; D020794:Receptor Protein-Tyrosine Kinases