Oxidative stress and adhesion molecules in children with type 1 diabetes mellitus: a possible link.
OBJECTIVE
To examine whether oxidative stress parameters were correlated with adhesion molecules derived from endothelial/platelet activation in a group of juveniles with type 1 diabetes mellitus (T1DM).


METHODS
Indicative parameters of patient oxidant/antioxidant capacity were measured and associated with P-selectin and tetranectin (TN), markers of endothelial/platelet activation, in the plasma of 45 diabetic children and adolescents and 20 healthy age-matched subjects (HS).


RESULTS
Significantly, higher nitrate/nitrite (NOx) and lipid hydroperoxide (LPO) levels (p=0.049 and p=0.0011, respectively), lower glutathione peroxidase activity (GPx; p=0.038), and elevated TN and P-selectin plasma levels (p=0.0046 and p=0.042, respectively) were found in T1DM children compared with HS. Well-controlled T1DM children (HbA1c <or= 7%) showed significantly lower GPx (p=0.0259), higher NOx and LPO (p=0.01093 and p=0.0092, respectively) compared with HS, while poorly controlled patients (HbA1c >7%) showed significantly higher TN, sP-selectin and LPO (p=0.0064, p=0.0234 and p=0.0121, respectively), a tendency to higher NOx (p=0.063) compared with HS and only TN higher (p=0.0123) compared with well-controlled patients. Patients with shorter diabetes duration (<or=3 yr) showed significantly higher LPO and TN (p=0.034 and 0.017, respectively), a tendency to higher NOx and lower GPx and higher P-selectin, while those with longer duration (>3 yr) differed significantly in all the examined parameters (TN, p=0.0015; GPx, p=0.0420; NOx, p=0.0196; LPO, p=0.0054; sP-selectin, p=0.0187) compared with HS.


CONCLUSIONS
Decreased antioxidative protection from simultaneous LPO and NOx overproduction is evident in T1DM juveniles with a parallel endothelial/platelet activation even in the first years of the disease, being more pronounced later in diabetes progression, contributing to the vascular complications of the disease.
Second Department of Pediatric Clinic, Aglaia Kyriakou Pediatric Hospital, Medical School, University of Athens, Athens, Greece.
Christina
C
Mylona-Karayanni
Dimitrios
D
Gourgiotis
Apostolos
A
Bossios
Elli F
EF
Kamper
D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D006442:Glycated Hemoglobin; D006801:Humans; D037181:Lectins, C-Type / Q000097:blood*; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D009569:Nitric Oxide / Q000097:blood*; D018384:Oxidative Stress / Q000502:physiology*; D019007:P-Selectin / Q000097:blood*
D015415:Biomarkers; D001798:Blood Proteins; D005979:Glutathione Peroxidase; D006442:Glycated Hemoglobin; D037181:Lectins, C-Type; D008054:Lipid Peroxides; D009569:Nitric Oxide; D019007:P-Selectin; C048648:tetranectin