DNA hydrolysis promoted by 1,7-dimethyl-1,4,7,10-tetraazacyclododecane.
Several acyclic and macrocyclic polyamines were evaluated for their ability to cleave DNA. 1,7-Dimethyl-1,4,7,10-tetraazacyclododecane (DMC) could hydrolyze double-strand DNA at a concentration of 25microM. pH 7.2 was the optimal condition to cleave DNA in the presence of DMC. Supercoiled DNA hydrolytic cleavage by DMC was supported by the evidence from free radical quenching and T4 ligase ligation.
Wan
Shu-Hui
SH
College of Chemistry and Molecular Sciences, Wuhan University, Hubei, Wuhan 430072, PR China.
Liang
Feng
F
Xiong
Xiao-Qin
XQ
Yang
Li
L
Wu
Xiao-Jun
XJ
Wang
Ping
P
Zhou
Xiang
X
Wu
Cheng-Tai
CT
D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006571:Heterocyclic Compounds / Q000494:pharmacology*; D006868:Hydrolysis
C512308:1,7-dimethyl-1,4,7,10-tetraazacyclododecane; D006571:Heterocyclic Compounds; D004247:DNA