Regulating the drought-responsive element (DRE)-mediated signaling pathway by synergic functions of trans-active and trans-inactive DRE binding factors in Brassica napus.
DREB1/C-repeat binding factor (CBF) is a plant-specific family of transcription factors and plays a crucial role in freeze tolerance. In the present work, two groups of drought-responsive element binding factor (DREB)-like genes were isolated from Brassica napus, named Group I and Group II. The two groups of genes were both induced by low temperature, but the expression of Group I preceded that of Group II. The Group I DREBs could specifically bind with the DRE cis-acting element and activate the expression of downstream genes, but Group II factors were trans-inactive although they still had the ability to bind with DRE, which was confirmed by electrophoretic mobility shift assay. Fluorescence quenching assays indicated that the DRE binding ability of the two groups was similar. Co-expression of Group II could depress the trans-activation activity of Group I DREB in a concentration-dependent manner. These results strongly suggested that the trans-active Group I DREBs were expressed at the early stage of cold stress to open the DRE-mediated signaling pathway in cold stress, whereas the trans-inactive Group II DREBs were expressed at the later stage to close the signal pathway in a competitive manner. The results herein provide a new insight into the regulation mechanisms of the DRE-mediated signaling pathway in response to cold stress.
Zhao
Tong-Jin
TJ
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
Sun
Shan
S
Liu
Yang
Y
Liu
Jing-Mei
JM
Liu
Qiang
Q
Yan
Yong-Bin
YB
Zhou
Hai-Meng
HM
D000595:Amino Acid Sequence; D015153:Blotting, Western; D029688:Brassica napus / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004190:Disasters; D004305:Dose-Response Relationship, Drug; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008954:Models, Biological; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013696:Temperature; D013997:Time Factors; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques
D018076:DNA, Complementary; D010940:Plant Proteins; D004247:DNA