Histone H4-K16 acetylation controls chromatin structure and protein interactions.
Acetylation of histone H4 on lysine 16 (H4-K16Ac) is a prevalent and reversible posttranslational chromatin modification in eukaryotes. To characterize the structural and functional role of this mark, we used a native chemical ligation strategy to generate histone H4 that was homogeneously acetylated at K16. The incorporation of this modified histone into nucleosomal arrays inhibits the formation of compact 30-nanometer-like fibers and impedes the ability of chromatin to form cross-fiber interactions. H4-K16Ac also inhibits the ability of the adenosine triphosphate-utilizing chromatin assembly and remodeling enzyme ACF to mobilize a mononucleosome, indicating that this single histone modification modulates both higher order chromatin structure and functional interactions between a nonhistone protein and the chromatin fiber.
Shogren-Knaak
Michael
M
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Ishii
Haruhiko
H
Sun
Jian-Min
JM
Pazin
Michael J
MJ
Davie
James R
JR
Peterson
Craig L
CL
D000107:Acetylation; D000255:Adenosine Triphosphate / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D004247:DNA / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D024202:Electrophoretic Mobility Shift Assay; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000378:metabolism; D015636:Magnesium Chloride / Q000494:pharmacology; D009707:Nucleosomes / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D002843:Chromatin; D029721:Drosophila Proteins; D006657:Histones; D009707:Nucleosomes; D011994:Recombinant Proteins; D014157:Transcription Factors; D015636:Magnesium Chloride; D000255:Adenosine Triphosphate; D004247:DNA; D008239:Lysine