Effective transfection of cells with multi-shell calcium phosphate-DNA nanoparticles.
Coated calcium phosphate nanoparticles were prepared for cell transfection. A calcium phosphate nanoparticle served as core which was then coated with DNA for colloidal stabilisation. The efficiency of transfection could be considerably increased by adding another layer of calcium phosphate on the surface, thereby incorporating DNA into the particle and preventing its degradation within the cell by lysosomes. A subsequent outermost layer of DNA on the calcium phosphate gave a colloidal stabilisation. The efficiency of such multi-shell particles was significantly higher than that of simple DNA-coated calcium phosphate nanoparticles. The transfection efficiency of EGFP-encoding DNA was tested with different cell lines (T-HUVEC, HeLa, and LTK). The dispersions were stable and could be used for transfection after 2 weeks of storage at 4 degrees C without loss of efficiency.
Sokolova
Viktoriya V
VV
Institute of Inorganic Chemistry, University of Duisburg-Essen, Universitaetsstrasse 5-7, D-45117 Essen, Germany.
Radtke
Ina
I
Heumann
Rolf
R
Epple
Matthias
M
D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D055672:Static Electricity; D013499:Surface Properties; D014162:Transfection / Q000379:methods*
D002130:Calcium Phosphates; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D004247:DNA