Cloning and sequencing of equine cardiac troponin I and confirmation of its usefulness as a target analyte for commercial troponin I analyzers.
The analysis of cardiac troponin I (cTnI) in the diagnosis of myocardial injury in domestic animals is gaining popularity. In this study, equine cTnI was sequenced and compared with previously characterized cTnI from other species. A 6-amino-acid N-terminal deletion unique to the horse was identified. This deletion was outside the epitope region of cTnI recognized by most commercial immunoassays and did not affect the ability of a commercial analyzer system to detect recombinant equine cTnI. No function could be ascribed to the deleted portion. These data support the use of commercial analyzers in measuring equine cTnI.
Department of Biomedical Sciences, VRT, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Mark
M
Rishniw
Kenny W
KW
Simpson
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006736:Horses / Q000235:genetics*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D011933:Reagent Kits, Diagnostic / Q000662:veterinary*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019210:Troponin I / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*
D011933:Reagent Kits, Diagnostic; D019210:Troponin I