[Experimental study on the proliferation and function of osteoblast cell induced by pBLAST49-mVEGF gene transfection].
OBJECTIVE
To assess the effects of extra VEGF gene expression on the proliferation of osteoblast cell.


METHODS
The extra VEGF gene was induced into osteoblast cell. Then the expression of extra VEGF gene was detected, and its effects on the cell proliferation curve, cell cycle, on the synthesis of type I collagen and on the secretion of osteocalcium of osteoblast cell were investigated.


RESULTS
At the first two days of culture, the cell number of pBLAST49-mVEGF gene transfer group was larger than that of the control group, yet no statistically significant difference was seen between the two groups (P > 0.05); however, significant difference was observed from the 3rd day of culture (P < 0.05). The (G2/M+S)% of the 1-5 generation osteoblast cell in pBLAST49-mVEGF gene transfer group was higher than that of the control group, there was significant difference between them (P < 0.05). The concentration of osteocalcium and expression of type I collagen of the 1-5 generation osteoblast cell in pBLAST49-mVEGF gene transfer group were significantly higher than those of the control group (P < 0.05).


CONCLUSIONS
pBLAST49-mVEGF gene transfer can improve the proliferation and function of osteoblast cell.
Zhong
Gang
G
Department of Orthopedic Surgery, West China Hostipal, Sichuan University, Chengdu 610041, China.
Pei
Fu-xing
FX
Li
Sheng-fu
SF
Fan
Yu-bo
YB
D000818:Animals; D000831:Animals, Newborn; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D007150:Immunohistochemistry; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
D024042:Collagen Type I; D042461:Vascular Endothelial Growth Factor A