The attachment of V79 and human periodontal ligament fibroblasts on periodontally involved root surfaces following treatment with EDTA, citric acid, or tetracycline HCL: an SEM in vitro study.
OBJECTIVE
The present in vitro study has been designed to establish and compare the effects of citric acid, EDTA, and tetracycline HCl on human periodontally diseased roots on the structure, attachment, and orientation of V79 (primary Chinese hamster lung fibroblasts) cells and human periodontal ligament fibroblasts (HPDL).


METHODS
Commercially available V79 cells and HPDL derived from healthy human third molars were used in this study. These fibroblasts were left in solution for seven days in order to attain confluence. Forty single-rooted teeth were obtained from patients diagnosed with periodontitis. The crown part was removed under constant irrigation and the root was split vertically into two equal halves, thus, yielding 80 specimens. Following scaling and root planing, the specimens were washed with phosphate buffered saline (PBS) and kept in 50 microg/ml gentamycin sulphate solution for 24 hours. The root pieces were then treated as follows: citric acid at pH 1, 24% EDTA, or with a 10% solution of tetracycline HCl and were then placed in V79 fibroblast cultures and HPDL cultures. The specimens were harvested after four weeks and were fixed in 2.5% glutaraldehyde in PBS before preparation for scanning electron microscopy (SEM).


RESULTS
The behavior of V79 cells was similar to that of human periodontal ligament cells on root conditioned surfaces. V79 and HPDL showed a healthy morphology on root surfaces treated with citric acid and EDTA and a relatively unhealthy appearance on root surfaces treated with tetracycline HCl and distilled water (control group).


CONCLUSIONS
The results suggest the use of citric acid and EDTA as root conditioning agents favorably affects the migration, attachment, and morphology of fibroblasts on human root surfaces, which may play a significant role in periodontal healing and regeneration.
Department of Periodontics, College of Dental Surgery, Manipal, India. viswachandra@hotmail.com
R Viswa
RV
Chandra
Ganesh Chandra
GC
Jagetia
K Mahalinga
KM
Bhat
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002461:Cell Line, Transformed; D049109:Cell Proliferation / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology*; D019343:Citric Acid / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D045662:Dental Etching; D004492:Edetic Acid / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D010518:Periodontitis / Q000503:physiopathology; D012038:Regeneration; D016369:Smear Layer; D013499:Surface Properties / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology; D014092:Tooth Root / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D002614:Chelating Agents; D019343:Citric Acid; D004492:Edetic Acid; D013752:Tetracycline