[Studies on the interaction between RNA with methyl violet and determination of RNA by spectrophotometry].
An analytical method for the determination of ribonucleic acid was established by spectrophotometry. At maximum absorption wavelength for methyl violet in the B-R buffer solution, and under the best conditions, the degree of decrease of the absorbance was linear with the amount of ribonucleic Acid. It was a new and preferable approach for the determination of ribonucleic Acids. The method with the linearity range was 1.0 to 8.0 microg x mL(-1) and the detection limit was 0.52 microg x mL(-1), and the correlation coeffient was 0.9999. This method was simple, rapid, and selective. So it was satisfactory to the application for the determination of ribonucleic Acid. The reaction mechanism was electrostatic interaction to make molecular association of RNA with methyl violet, to go on antiion permutation and bonded reaction of concert.
Si
Wen-hui
WH
Suzhou Agriculture College, Suzhou 215008, China.
Zi
Yan-qin
YQ
D005840:Gentian Violet / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012313:RNA / Q000032:analysis* / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*
D012313:RNA; D005840:Gentian Violet