Human herpesvirus 7 U47 gene products are glycoproteins expressed in virions and associate with glycoprotein H.
The function of the human herpesvirus 7 (HHV-7) U47 gene, which is a positional homologue of the genes encoding glycoprotein O (gO) in human cytomegalovirus (HCMV) and human herpesvirus 6 (HHV-6), was analysed. A monoclonal antibody (mAb) against the U47 gene product reacted in immunoblots with proteins migrating at 49 and 51 kDa in lysates of HHV-7-infected cells and with 49 and 51 kDa proteins in partially purified virions. Digestion of the 49 and 51 kDa proteins with endoglycosidase H and peptide N-glycosidase F indicated that the U47-encoded proteins were modified with N-linked oligosaccharides. Therefore, the U47 gene and its product were named gO, as in HCMV and HHV-6. In addition, the anti-gO mAb co-immunoprecipitated glycoprotein H (gH) in HHV-7-infected cells, indicating an association between HHV-7 gO and gH. The results suggest that the HHV-7 gO-gH complex might have a similar function to that in HCMV or HHV-6, such as cell-cell fusion in virus infection.
Sadaoka
Tomohiko
T
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
Yamanishi
Koichi
K
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
Mori
Yasuko
Y
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
D015153:Blotting, Western; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D016199:Herpesvirus 7, Human / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism*; D014771:Virion / Q000378:metabolism*
D006023:Glycoproteins; D014759:Viral Envelope Proteins