Role of CXC chemokine receptor 3 pathway in renal ischemic injury.
Chemokines play a major role in the recruitment of leukocytes in inflammation and in the regulation of T helper 1 (Th1)/Th2 immune responses. These mechanisms have been recognized to be important in the pathogenesis of renal ischemia-reperfusion (I/R) injury. The interaction of the CXC chemokine receptor 3 (CXCR3) receptor with its ligands is a key pathogenic pathway in promoting inflammation and in enhancing Th1 immune responses. After the induction of ischemia in the mouse model of renal ischemia, an increase in intrarenal expression of CXCR3 and its ligands was observed. Compared with the wild-type (WT) mice, CXCR3-deficient mice (CXCR3-/-) had significantly lower serum creatinine levels, better survival rate, and significantly less acute tubular necrosis and cellular infiltrates. In the kidney, intracellular staining of infiltrating cells that were recovered from kidneys revealed a lower percentage of CD4+IFN-gamma+ cells in the CXCR3-/- mice compared with the WT mice. Furthermore, adoptive transfer of WT CD3+ cells into CXCR3-/- mice before induction of I/R injury abrogated the protection of CXCR3-/- mice from I/R injury. It is concluded that CXCR3 plays an important role in orchestrating the recruitment of Th1 cells to the ischemic kidney and in mediating I/R injury and therefore may serve as a novel target for the therapy of I/R injury.
Transplantation Research Center, Children's Hospital, Harvard Medical School, 221 Longwood Avenue, Boston, MA 02115, USA.
Paolo
P
Fiorina
Mohammed Javeed
MJ
Ansari
Mollie
M
Jurewicz
Mark
M
Barry
Vincent
V
Ricchiuti
Rex Neal
RN
Smith
Susan
S
Shea
Terry K
TK
Means
Hugh
H
Auchincloss
Andrew D
AD
Luster
Mohamed H
MH
Sayegh
Reza
R
Abdi
D000704:Analysis of Variance; D000818:Animals; D001707:Biopsy, Needle; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D007674:Kidney Diseases / Q000276:immunology / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011336:Probability; D012313:RNA / Q000032:analysis / Q000378:metabolism; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000032:analysis / Q000378:metabolism*; D012016:Reference Values; D015427:Reperfusion Injury / Q000276:immunology / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
C515188:Cxcr3 protein, mouse; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine; D012313:RNA