Production of poly(L-lactide)-degrading enzyme by Amycolatopsis orientalis for biological recycling of poly(L-lactide).
Efficient production of poly(L-lactide)(PLA)-degrading enzyme was achieved by addition of 0.1% (w/v) silk fibroin powder into a liquid culture medium of an actinomycete, Amycolatopsis orientalis, without other complex nitrogen sources, such as yeast extract and peptone. Scaled-up production of the enzyme in a 5-l jar fermenter showed the possibility of producing this enzyme on an industrial scale at low production cost. The extracellular PLA-degrading enzyme showed potent degrading activity, which is effective for biological recycling of PLA, i.e., 2,000 mg/l of PLA powder was completely degraded within 8 h at 40 degrees C using 20 mg/l purified enzyme. An optically active L-lactic acid with 600 mg/l was obtained as degradation product of PLA without undesirable racemization.
CPR Co., Ltd., 4-102, Higashinobusue, Himeji, Hyogo, 670-0965, Japan.
Amnat
A
Jarerat
Yutaka
Y
Tokiwa
Hideo
H
Tanaka
D000192:Actinomycetales / Q000201:enzymology* / Q000254:growth & development; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003247:Conservation of Natural Resources; D003470:Culture Media / Q000737:chemistry; D011091:Polyesters / Q000378:metabolism*
D003470:Culture Media; D011091:Polyesters; C033616:poly(lactide); D002265:Carboxylic Ester Hydrolases