Lymph node metastasis is infrequent in patients with highly-methylated colorectal cancer.
In this study, the combined methylation status of p16, p14, HLTF, CDH13 and RUNX3 was examined in 59 resected primary colorectal cancers using methylation-specific PCR and the methylation status was correlated with the clinicopathological features of the affected patients. A significant difference was found in lymph node metastasis (p = 0.0359) on comparing the methylation status in colorectal cancers with lymph node metastasis to those without. There was also a significant gender difference (p = 0.0248) when the methylation status was compared. Colorectal cancer without lymph node metastasis frequently exhibited aberrant methylation of the p16, p14, HLTF, CDH13 and RUNX3 genes.
Department of Surgery, Seirei Hospital, 56 Kawanayama-machi, Showa-ku, Nagoya 466-8633, Japan. khibi@med.nagoya-u.ac.jp
Kenji
K
Hibi
Akimasa
A
Nakao
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015820:Cadherins / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D019942:Genes, p16; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics
D015820:Cadherins; D050677:Core Binding Factor Alpha 3 Subunit; C099972:H-cadherin; C493729:Runx3 protein, human; D025803:Tumor Suppressor Protein p14ARF