[Determination of propofol in cerebral spinal fluid by HPLC with fluorescence detection].
To establish a method for determining propofol in human cerebrospinal fluid (CSF).



Reverse phase high-performance liquid chromatography (HPLC) with fluorescence detection was applied to quantitative analysis. CSF samples were centrifuged (12,500 r/min for 3 min) and filtered (the diameter of the filter is 0.45 microm). Twenty mul of supernatant was directly injected and separated by Supelco Discovery C(18)column. The mobile phase was composed of methanol-water (80:20); the flow rate was 1 ml/min, and the column temperature was 30 degree. The fluorescence detective waves were: lambda ex=276 nm and lambda em=310 nm.



The linear range of propofol in CSF was 5-200 ng/ml (r=0.9994). The recovery rates for high, intermediate and low concentrations were 101.2%, 99.8%, 98.8%, respectively. The RSD of inter-day assay was 1.55%, 1.73%, 6.01% and it of intra-day assay was 1.69%, 2.37%, 8.60%. The limit of detection proved to be 2 ng/ml.



The method is rapid, simple, accurate and sensitive for measurement of propofol concentration in CSF.
Department of Anesthesiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. yuh_li2002@hotmail.com
Yu-hong
YH
Li
Fang-ping
FP
Bao
Sheng-mei
SM
Zhu
Jian-guo
JG
Xu
D018686:Anesthetics, Intravenous / Q000134:cerebrospinal fluid*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D015742:Propofol / Q000134:cerebrospinal fluid*; D013050:Spectrometry, Fluorescence
D018686:Anesthetics, Intravenous; D015742:Propofol