[A molecular-and-genetic analysis of species and strain diversity of bifidobacteria in early childhood].
The species and strain composition of bifidobacteria in 29 children both sexes, aged 8 to 16 months, was studied. Species-specific primers and PCR were used to determine to which species the predominant strains of bifidobacteria, isolated from feces by cultural methods, belonged. Bifidobacteria were found in 28 (96.5%) children; their number was 10.2 +/- 0.7 ECU per a gram of the material. B. longum and B. bifidum were frequent (71.4 and 53.5%, respectively). The level of quantitative detection used in the study also allowed revealing of B. catenulatum (17.9%) and B. breve (14.4%). A high titer of B. dentium was found in one case (3.6%). B. adolescentis and B. angulatum were not found in any patient. The average number of species found in one child was 1.7 +/- 0.7. RAPD-PCR and investigation of plasmid profile were used to determine possible belonging of the isolates to different strains. The average number of strains per one sample was 2.3 +/- 1.2. Nine unique plasmid bifidobacterial strains were isolated from 7 children. In 3 children the intestinal tract was found to be colonized by both plasmid and non-plasmid-carrying strains of one bifidobacterial species.
Shkoporov
A N
AN
Kafarskaia
L I
LI
Afanas'ev
S S
SS
Smeianov
V V
VV
Kirillov
M Iu
MIu
Postnikova
E A
EA
Maksimov
F E
FE
Khokhlova
E V
EV
Efimov
B A
BA
D000367:Age Factors; D001431:Bacteriological Techniques; D001644:Bifidobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D007422:Intestines / Q000382:microbiology; D008297:Male; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012737:Sex Factors
D017931:DNA Primers; D004269:DNA, Bacterial