Analysis of p53 protein by PAb240, Ki-67 expression and human papillomavirus DNA detection in different types of odontogenic keratocyst.
OBJECTIVE
To investigate the possible association between alterations in the p53 system and human papillomavirus (HPV) infection in the etiology of odontogenic keratocysts (OKCs) and to study proliferation and epithelial maturation patterns by topographic analysis of Ki-67 expression.


METHODS
Eighty-three OKC samples (29 cases associated with nevoid basal cell carcinoma syndrome, 29 solitary non-recurrent cases 20 solitary recurrent cases, and 5 chondroid keratocysts) were studied by immunohistochemistry to detect p53 protein (PAb 244) and Ki-67 (MIB-1) expression, and by PCR to detect HPV DNA.


RESULTS
Twelve cases (14.6%) expressed p53 protein; no case showed the presence of HPV DNA; 9 cases (11%) presented with mild epithelial dysplasia. The suprabasal expression of Ki-67 was significantly more frequent than its basal expression (p < 0.001). p53 protein expression was significantly associated with the presence of epithelial dysplasia (p = 0.023). Ki-67 expression was not associated with OKC type, the presence of dysplasia, or p53 expression.


CONCLUSIONS
HPVs do not participate in the etiology of OKC, and it appears unlikely that a p53 gene mutation mechanism plays a major role in the genesis of OKC. OKCs show proliferation and genuine maturation behavior reminiscent of benign neoplasms with local destructive capacity.
Oral Medicine, Department of Stomatology, University of Granada, Granada, Spain. magonzal@ugr.es
Miguel A
MA
González-Moles
Adalberto
A
Mosqueda-Taylor
Miguel
M
Delgado-Rodríguez
Guillermo
G
Martínez-Mata
José Antonio
JA
Gil-Montoya
Marco Antonio
MA
Díaz-Franco
José Javier
JJ
Bravo-Pérez
Nabila
N
M-González
D002280:Basal Cell Carcinoma / Q000378:metabolism / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D008297:Male; D009807:Odontogenic Cysts / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
D004279:DNA, Viral; D019394:Ki-67 Antigen; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53