Oxidative stress and adhesion molecules in children with type 1 diabetes mellitus: a possible link.
OBJECTIVE
To examine whether oxidative stress parameters were correlated with adhesion molecules derived from endothelial/platelet activation in a group of juveniles with type 1 diabetes mellitus (T1DM).


METHODS
Indicative parameters of patient oxidant/antioxidant capacity were measured and associated with P-selectin and tetranectin (TN), markers of endothelial/platelet activation, in the plasma of 45 diabetic children and adolescents and 20 healthy age-matched subjects (HS).


RESULTS
Significantly, higher nitrate/nitrite (NOx) and lipid hydroperoxide (LPO) levels (p=0.049 and p=0.0011, respectively), lower glutathione peroxidase activity (GPx; p=0.038), and elevated TN and P-selectin plasma levels (p=0.0046 and p=0.042, respectively) were found in T1DM children compared with HS. Well-controlled T1DM children (HbA1c <or= 7%) showed significantly lower GPx (p=0.0259), higher NOx and LPO (p=0.01093 and p=0.0092, respectively) compared with HS, while poorly controlled patients (HbA1c >7%) showed significantly higher TN, sP-selectin and LPO (p=0.0064, p=0.0234 and p=0.0121, respectively), a tendency to higher NOx (p=0.063) compared with HS and only TN higher (p=0.0123) compared with well-controlled patients. Patients with shorter diabetes duration (<or=3 yr) showed significantly higher LPO and TN (p=0.034 and 0.017, respectively), a tendency to higher NOx and lower GPx and higher P-selectin, while those with longer duration (>3 yr) differed significantly in all the examined parameters (TN, p=0.0015; GPx, p=0.0420; NOx, p=0.0196; LPO, p=0.0054; sP-selectin, p=0.0187) compared with HS.


CONCLUSIONS
Decreased antioxidative protection from simultaneous LPO and NOx overproduction is evident in T1DM juveniles with a parallel endothelial/platelet activation even in the first years of the disease, being more pronounced later in diabetes progression, contributing to the vascular complications of the disease.
Mylona-Karayanni
Christina
C
Second Department of Pediatric Clinic, Aglaia Kyriakou Pediatric Hospital, Medical School, University of Athens, Athens, Greece.
Gourgiotis
Dimitrios
D
Bossios
Apostolos
A
Kamper
Elli F
EF
D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D006442:Glycated Hemoglobin; D006801:Humans; D037181:Lectins, C-Type / Q000097:blood*; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D009569:Nitric Oxide / Q000097:blood*; D018384:Oxidative Stress / Q000502:physiology*; D019007:P-Selectin / Q000097:blood*
D015415:Biomarkers; D001798:Blood Proteins; D005979:Glutathione Peroxidase; D006442:Glycated Hemoglobin; D037181:Lectins, C-Type; D008054:Lipid Peroxides; D009569:Nitric Oxide; D019007:P-Selectin; C048648:tetranectin