[Construction of a cDNA library of Alternaria sp. strain JH505 using Gateway technology].
A cDNA library was constructed based on the Gateway system that is a method to construct a high-quality cDNA library without the use of restriction enzyme for cloning. This is the first report that the Gateway system is used to construct a cDNA library for Alternaria sp.. The entry cDNA library was constructed in this report has a high titer of 1 x 10(7) cfu/mL and contain a total clones of 9 x 10(7) cfu, with an average inserts size of about 1510bp. In order to screen for a gene encoding the plant activator protein from library using an antiserum, the entry cDNA library was transferred into Gateway destination vector to create an expression library. The expression library show a high titer of 1.58 x 10(6) cfu/mL and contains a total clones of 6.32 x 10(6) cfu, with an average inserts size of 1680bp.
Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China. longsonghua79452@sohu.com
Song-Hua
SH
Long
Ning
N
Zhang
De-Wen
DW
Qiu
Ding-Jun
DJ
Li
Wei
W
Huang
D000528:Alternaria / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D015723:Gene Library; D005821:Genetic Techniques; D011995:Recombination, Genetic