Stimulatory effect of lactic acid bacteria from commercially available Nozawana-zuke pickle on cytokine expression by mouse spleen cells.
We investigated the effect of lactic acid bacteria (LAB) isolated from eight samples of commercially available Nozawana-zuke, a traditional Japanese pickle, on cytokine expression by mouse spleen cell cultures. The 12 isolated strains of LAB (Nz1-Nz12), which were identified as genus Lactobacillus or Leuconostoc by the API50CHL test, enhanced the expression of interferon (IFN)-gamma with 6 h of culture. Ten of these 12 LAB, particularly Nz8, enhanced interleukin (IL)-12 p40 expression. The actinase E- and Benzonase-treated or untreated cell wall fraction of Nz8 enhanced both IFN-gamma and IL-12 p40 expression, while the cell plasma fraction had little effect. In the presence of anti-toll like receptor 4 antibody, the effect of the cell wall fraction of Nz8 was significantly abrogated. These results suggest that some LAB from Nozawana-zuke have a T helper 1-type immunoenhancing effect.
Kawahara
Takeshi
T
Integrated Department of Sciences of Functional Foods, Graduate School of Agriculture, Shinshu University, Japan. tkawafb@shinshu-u.ac.jp
Otani
Hajime
H
D000818:Animals; D000906:Antibodies / Q000276:immunology; D019607:Brassicaceae / Q000382:microbiology*; D002473:Cell Wall / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005285:Fermentation; D005786:Gene Expression Regulation; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus / Q000145:classification / Q000378:metabolism*; D007934:Leuconostoc / Q000145:classification / Q000378:metabolism*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013154:Spleen / Q000166:cytology*; D051193:Toll-Like Receptors / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D016207:Cytokines; D051193:Toll-Like Receptors; D019344:Lactic Acid