Characterization of L-arginine transport in adrenal cells: effect of ACTH.
Nitric oxide synthesis depends on the availability of its precursor L-arginine, which could be regulated by the presence of a specific uptake system. In the present report, the characterization of the L-arginine transport system in mouse adrenal Y1 cells was performed. L-arginine transport was mediated by the cationic/neutral amino acid transport system y+L and the cationic amino acid transporter (CAT) y+ in Y1 cells. These Na+-independent transporters were identified by their selectivity for neutral amino acids in both the presence and absence of Na+ and by the effect of N-ethylmaleimide. Transport data correlated to expression of genes encoding for CAT-1, CAT-2, CD-98, and y+LAT-2. A similar expression profile was detected in rat adrenal zona fasciculata. In addition, cationic amino acid uptake in Y1 cells was upregulated by ACTH and/or cAMP with a concomitant increase in nitric oxide (NO) production.
Repetto
Esteban M
EM
Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
Pannunzio
Vanesa
V
Astort
Francisco
F
Martinez Calejman
Camila
C
Moreno
Marcos Besio
MB
Pignataro
Omar P
OP
Cymeryng
Cora B
CB
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000008:administration & dosage*; D026923:Amino Acid Transport Systems, Basic / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D000242:Cyclic AMP / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats
D026923:Amino Acid Transport Systems, Basic; D009569:Nitric Oxide; D000324:Adrenocorticotropic Hormone; D001120:Arginine; D000242:Cyclic AMP