[The effect of fungi in the pathogenesis of nasal polyps and chronic rhinosinusitis].
OBJECTIVE
To study the effect of fungi in the pathogenesis of nasal polyps and chronic rhinosinusitis (CRS).


METHODS
This study were divided two groups, the first was the experimental group: Nasal mucus membrane and nasal polyps were collected from patients with CRS (with and without polyps), and the second was the control group, nasal mucus were collected from OHc. Fungal-specific PCR were performed on each sample. The anther was used 0.9% sol sodium chloride to wash the autopsy tissues 5 times, and then PCR analysis was performed. chi-squared analysis was used to test for statistical difference between these groups.


RESULTS
PCR technique detected fungal DNA were 89.2% and 89.4% in nasal polyps and nasal mucous membrane respectively, and 66.7% in normal control subjective. There were statistically significant difference in these groups. After using 0.9% sol sodium chloride to wash the autopsy tissues, the fungi's positive rate was dropped.


CONCLUSIONS
Fungi was important in the pathogenesis of nasal polyps and CRS.
Zhang
Qingjun
Q
Department of Otorhinolaryngology-Head and Neck Surgery, the First Affiliated Hospital of Beifang College, Zhangjiakou, 075000, China.
Li
Xuepei
X
Zhu
Li
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002908:Chronic Disease; D004271:DNA, Fungal / Q000032:analysis; D005260:Female; D005658:Fungi / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000382:microbiology; D009298:Nasal Polyps / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012852:Sinusitis / Q000382:microbiology*; D055815:Young Adult
D004271:DNA, Fungal