Preparation of tumor homogenates for subsequent preparation of cytosols, membrane fractions, RNA, and DNA.
This chapter outlines methods for the preparation of several different cellular fractions from whole samples of tumor and normal tissue.
Robertson
Naomi
N
Cancer Research UK Molecular Oncology Laboratories, Weatherall Institute of Molecular Medicine, The John Radcliffe Hospital, Oxford, UK.
Leek
Russell
R
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D003600:Cytosol / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D006801:Humans; D009369:Neoplasms / Q000737:chemistry*; D012313:RNA / Q000032:analysis*
D012313:RNA; D004247:DNA