Four gene products are required for the fungal synthesis of the indole-diterpene, paspaline.
Paspaline belongs to a large, structurally and functionally diverse group of indole-diterpenes synthesized by filamentous fungi. However, the identity of the gene products required for the biosynthesis of paspaline, a key intermediate for the synthesis of paxilline and other indole-diterpenes, is not known. Transfer of constructs containing different pax gene combinations into a paxilline negative deletion derivative of Penicillium paxilli demonstrated that just four proteins, PaxG, a geranylgeranyl diphosphate synthase, PaxM, a FAD-dependent monooxygenase, PaxB, a putative membrane protein, and PaxC, a prenyl transferase, are required for the biosynthesis of paspaline.
Saikia
Sanjay
S
Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North 5301, New Zealand.
Parker
Emily J
EJ
Koulman
Albert
A
Scott
Barry
B
D004122:Dimethylallyltranstransferase / Q000235:genetics; D004224:Diterpenes / Q000378:metabolism*; D051231:Farnesyltranstransferase / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D017353:Gene Deletion; D005800:Genes, Fungal; D007211:Indoles / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics; D010105:Oxygenases / Q000235:genetics; D010407:Penicillium / Q000235:genetics / Q000378:metabolism*
D004224:Diterpenes; D005656:Fungal Proteins; D007211:Indoles; D008565:Membrane Proteins; C048221:paspaline; D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming); D004122:Dimethylallyltranstransferase; D051231:Farnesyltranstransferase