Clastogenicity of the fungicide afugan in cultured human lymphocytes.
The genotoxic effects of the fungicide afugan were analysed by measuring chromosomal aberrations (CAs), sister chromatid exchange (SCE) and micronuclei (MN) in cultured human peripheral lymphocytes. Concentrations of 2.5, 5, 10 and 20 microg/ml of afugan were used during 24 and 48 h. Afugan significantly increased the frequency of CAs at 5, 10 and 20 microg/ml concentrations during a 48 h treatment period. A significant increase was observed for induction of SCE and MN at all treatments compared with the negative control. A significant dose-response correlation was found in all tests. Afugan did not affect the replicative index (RI), however it significantly decreased the mitotic index (MI) at all treatment concentrations except 2.5 microg/ml, and at both treatment times. The present results indicate that afugan is clastogenic and cytotoxic to cultured human lymphocytes.
Yüzbaşioğlu
Deniz
D
Gazi Universitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Teknikokullar, 06500 Ankara, Turkey.
Celik
Mustafa
M
Yilmaz
Serkan
S
Unal
Fatma
F
Aksoy
Hüseyin
H
D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005659:Fungicides, Industrial / Q000633:toxicity*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D015162:Micronucleus Tests; D008940:Mitotic Index; D009153:Mutagens / Q000633:toxicity*; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D014992:Xylenes / Q000633:toxicity*
D005659:Fungicides, Industrial; D009153:Mutagens; D009946:Organothiophosphorus Compounds; D014992:Xylenes; C511283:afugan