[A simple and repeatable model of subarachnoid hemorrhage in rats].
OBJECTIVE
To build a simple and repeatable animal model of subarachnoid hemorrhage (SAH).


METHODS
SAH was produced by passing a nylon thread up through the right internal carotid artery and piercing a hole in the right anterior cerebral artery. At 12 h and 24 h after SAH operation, the rats were evaluated with rotarod test and the behavior scale (5-point scale).


RESULTS
The rats were trained through rotarod test and then randomly divided into three groups, including vehicle group treated with vehicle after SAH, nimodipine treated group (i.p. 0.25 mg x kg(-1), 5 min, 6 h, 12 h after SAH) and sham group. At the point of the perforation there was usually a capping clot. There was always blood in the basal cisterns with some spread over the hemisphere. After 12 h and 24 h of SAH operation, the time of rotarod test of rats decreased significantly and the rats had serious neurological deficit. Nimodipine could alleviate the neurological deficit after 24 h of SAH.


CONCLUSIONS
To present a simple and reliable model of SAH in the rats, which allows evaluating novel compounds and new drugs for treatment of SAH.
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Liao-liao
LL
Li
Ling
L
Wang
Nan
N
Feng
Xiao-liang
XL
Wang
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000473:pathology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002560:Cerebrovascular Circulation / Q000187:drug effects; D004195:Disease Models, Animal; D008297:Male; D009553:Nimodipine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D045442:Rotarod Performance Test; D013345:Subarachnoid Hemorrhage / Q000209:etiology / Q000473:pathology / Q000503:physiopathology
D002121:Calcium Channel Blockers; D009553:Nimodipine