Temporal change in local forces and total force all over the surface of the sea urchin egg during cytokinesis.
We determined the tension over the entire surface of the sea urchin eggs during cytokinesis, on the basis of the intracellular pressure and cell shape. This allowed us to determine the temporal changes in both the distribution of local forces and the total force produced in the whole cell cortex. A spike-like peak at anaphase and a broader peak at the onset of furrowing were observed in the time-course of the total force. Treatment of the eggs with cytochalasin D, blebbistatin, ML-9, or ML-7 significantly lowered the total force when they inhibited cytokinesis, suggesting that the tension results mainly from the interaction between intact actin filaments and activated myosin II. Myosin II would function as a motor, not only in the furrow region, but over a wide area of the cell surface, because the sum of the tensions outside the furrow region was larger than that inside the furrow region throughout cytokinesis. The distribution of the local force revealed that a global increase in the cortical force started well before the onset of furrowing, and that the force inside the furrow region continued to increase despite the decrease in the force outside the furrow region after the onset of furrowing. The spatial and temporal patterns of the force over the entire surface support the hypothesis that there are two separate but coordinated actomyosin activation mechanisms, one of which induces global activation of the cortex and the other of which then maintains the contractility only inside the furrow region.
Miyoshi
Hiromi
H
Department of Bioengineering, Tokyo Institute of Technology, O-okayama, Tokyo, Japan.
Satoh
Setsuko K
SK
Yamada
Eio
E
Hamaguchi
Yukihisa
Y
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology*; D015638:Cytochalasin D / Q000494:pharmacology; D048749:Cytokinesis / Q000187:drug effects / Q000502:physiology*; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D024462:Myosin Type II / Q000502:physiology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D010063:Ovum / Q000502:physiology*; D012617:Sea Urchins / Q000502:physiology*; D008941:Spindle Apparatus / Q000187:drug effects / Q000502:physiology; D013500:Surface Tension; D013997:Time Factors
D006576:Heterocyclic Compounds, 4 or More Rings; D019384:Nucleic Acid Synthesis Inhibitors; C472645:blebbistatin; D015638:Cytochalasin D; D024462:Myosin Type II