The multicopper oxidase CutO confers copper tolerance to Rhodobacter capsulatus.
The cutO gene of the photosynthetic purple bacterium Rhodobacter capsulatus codes for a multicopper oxidase as demonstrated by the ability of the recombinant Strep-tagged protein to oxidize several mono- and diphenolic compounds known as substrates of Escherichia coli CueO and multicopper oxidases from other organisms. The R. capsulatus cutO gene was shown to form part of a tri-cistronic operon, orf635-cutO-cutR. Expression of the cutO operon was repressed under low copper conditions by the product of the cutR gene. CutO conferred copper tolerance not only under aerobic conditions, as described for the well-characterized E. coli multicopper oxidase CueO, but also under anaerobic conditions.
Lehrstuhl für Biologie der Mikroorganismen, Fakultät für Biologie, Ruhr-Universität Bochum, Bochum, Germany.
Jessica
J
Wiethaus
Günter F
GF
Wildner
Bernd
B
Masepohl
D002352:Carrier Proteins / Q000235:genetics; D003300:Copper / Q000502:physiology*; D004252:DNA Mutational Analysis / Q000379:methods; D004274:DNA, Recombinant / Q000235:genetics; D004361:Drug Tolerance / Q000502:physiology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D023061:Gene Order; D016297:Mutagenesis, Site-Directed / Q000379:methods; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D012329:RNA, Bacterial / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016354:Rhodobacter capsulatus / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D001616:beta-Galactosidase / Q000032:analysis
D002352:Carrier Proteins; D004274:DNA, Recombinant; D012329:RNA, Bacterial; C023548:copper-binding protein; D003300:Copper; D010088:Oxidoreductases; C024748:copper oxidase; D001616:beta-Galactosidase