Immunostimulation of white shrimp (Litopenaeus vannamei) following dietary administration of Ergosan.
Ergosan an algal product containing 1% alginic acid, developed for use in aquaculture and reported to have immunomodulatory activity, was administered orally to intermoult adult white shrimp (Litopenaeus vannamei) for 15 days. Examination of haemolymph proteins using SDS-PAGE did not reveal any obvious differences between control and Ergosan treated shrimp. Similarly, total haemocyte counts were found to be roughly equivalent for both the control and experimental samples. However, differential analysis of haemocyte populations revealed marked changes in terms of the relative levels of hyaline, semi-granular, and particularly granular haemocytes between the two groups. Moreover, enhancement of the in vitro antimicrobial activity of haemolymph towards two shrimp pathogenic Vibrio isolates was recorded for shrimp fed with Ergosan. Finally, shrimp fed with Ergosan showed a significant increase in relative growth when compared with control groups.
Instituto Nacional de la Pesca, SAGARPA, Pitágoras 1320 CP, 03310 México, D.F., Mexico. anabmont@servidor.unam.mx
A
A
Montero-Rocha
D
D
McIntosh
R
R
Sánchez-Merino
I
I
Flores
D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D000900:Anti-Bacterial Agents; D033364:Decapoda / Q000187:drug effects / Q000502:physiology*; D020723:Glucuronic Acid / Q000008:administration & dosage; D006434:Hemocytes / Q000737:chemistry*; D006458:Hemolymph / Q000737:chemistry* / Q000502:physiology; D006603:Hexuronic Acids / Q000008:administration & dosage; D007113:Immunity, Innate / Q000187:drug effects*; D007114:Immunization; D014733:Vibrio / Q000187:drug effects / Q000254:growth & development; D014736:Vibrio parahaemolyticus
D000464:Alginates; D000900:Anti-Bacterial Agents; D006603:Hexuronic Acids; D020723:Glucuronic Acid