Th1 bias in PBMC induced by multicycles of auto-CIKs infusion in malignant solid tumor patients.
OBJECTIVE
The aim of this paper was to verify the feasibility and validity of autologous cytokine-induced killer cells (Auto-CIKs) treatments in solid malignancy patients.


METHODS
Amplification, phenotypic characteristics, antitumor cytotoxicity, and clinical and immunological response of Auto-CIKs derived from 66 cases of patients with solid tumor of different pathological types and at different clinical stages were examined in a large-scale clinical trail.


RESULTS
We found that seriousness of disease and metastasis status has no influence on effective components and antitumor activity of Auto-CIKs. Comparing the cytotoxicity against various tumor cells with LAKs, CIKs showed more effective cytotoxicity against NK sensitive and nonsensitive solid tumor cell lines, even at a low E/T ratio (6:1). In 20 patients receiving multicycles of Auto-CIKs infusions, 3 reached partial response (PR), 14 obtained stable disease (SD), and 3 died. Th1-kind cytokines secretion in peripheral blood mononuclear cells (PBMCs increased significantly, according with the enhanced cytolytic activity against K562 cells of them after multicycles of Auto- CIKs infusions.


CONCLUSIONS
Induction of special "Th1 bias" in PBMCs after multicycles of Auto-CIKs treatments suggested an immunological promoting effect of Auto-CIKs, which seems to be a suitable immunotherapy for those solid-malignance patients who are at high risk of relapse to prevent recurrence.
Ren
Xiubao
X
Department of Immunology, Tianjin Cancer Institute and Hospital, Tianjin, China. rwziyi@yahoo.com
Yu
Jinpu
J
Liu
Hong
H
Zhang
Peng
P
An
Xiumei
X
Zhang
Naining
N
Hao
Xishan
X
D000293:Adolescent; D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D016207:Cytokines / Q000276:immunology; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000637:transplantation*; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D014182:Transplantation, Autologous
D016207:Cytokines