Role of Kupffer cells in acute hemorrhagic necrotizing pancreatitis-associated lung injury of rats.
OBJECTIVE
To investigate the role of Kupffer cells (KCs) in acute hemorrhagic necrotizing pancreatitis-associated lung injury (AHNP-LI).


METHODS
Forty-two rats were allocated to four groups [sham operation, AHNP model, gadolinium chloride (GdCl3) pretreatment, GdCl3 control]. In GdCl3 pretreatment group, GdCl3 was administered by caudal vein injection 24 h before the AHNP model induction. Blood from the iliac artery, alveolar macrophages and tissues from the pancreas and lung, were collected in six animals per group 3 and 6 h after acute pancreatitis induction. TNF-alpha, IL-1 of serum, myeloperoxidase (MPO) of lung tissue, NF-kappaB activation of alveolar macrophages were detected. Serum AST and ALT in sham operation group and GdCl3 control group were tested. In addition, histopathological changes of the pancreas and lung were observed under light microscope.


RESULTS
MPO of lung tissue and TNF-alpha, IL-1 levels of serum were all reduced significantly in GdCl3 pretreatment group compared to those in AHNP group (P<0.01). NF-kappaB activation of alveolar macrophages was also attenuated significantly in GdCl3 pretreatment group compared to that in AHNP group (P<0.01). The pathological injury of the lung was ameliorated obviously in GdCl3 pretreatment group compared to that in AHNP group. Nevertheless, the serum amylase level did not reduce and injury of the pancreas was not prevented in GdCl3 pretreatment group.


CONCLUSIONS
Pulmonary injury induced by AHNP is mediated by KC activation and AHNP-LI can be significantly ameliorated by pretreatment with GdCl3 and KCs play a vital role in AHNP-LI.
Department of Pharmacology, Tianjin Institute of Acute Abdominal Diseases, No. 122, Sanwei Road, Nankai District, Tianjin 300100, China. jtsstj1@yahoo.com
Hong-Bin
HB
Liu
Nai-Qiang
NQ
Cui
Dong-Hua
DH
Li
Chang
C
Chen
D000681:Amylases / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D005682:Gadolinium / Q000627:therapeutic use; D007375:Interleukin-1 / Q000097:blood; D007728:Kupffer Cells / Q000166:cytology / Q000276:immunology*; D008168:Lung; D008297:Male; D019283:Pancreatitis, Acute Necrotizing / Q000150:complications* / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012128:Respiratory Distress Syndrome / Q000188:drug therapy / Q000209:etiology* / Q000276:immunology* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D005682:Gadolinium; D009195:Peroxidase; D000681:Amylases; C038958:gadolinium chloride