[Effects of N-linked oligosaccharide chains on expression of integrin beta1 and apoptosis in glomerular mesangial cells].
OBJECTIVE
The effects of N-linked oligosaccharide chains (TM), an inhibitor of N-glycosylation of proteins, on the expression of beta1 integrin and the apoptosis of glomerular mesangial cells (GMC) were observed for exploring the role of inhibitors of N-linked oligosaccharide chains in cell apoptosis, proliferation, and expression of beta1 integrin and cyclin D1, and hence finding a new approach to the synteresis of proliferative nephropathy.


METHODS
Cultured rat mesangial cells were divided into 4 groups: control group, and 0.5, 1.0 and 2.0 microg/mL TM-treatment groups. The expression of beta1 integrin and apoptosis rate were measured by flow cytometry; the expression of cyclin D, was measured by immunohistochemistry, and proliferation of GMCs was measured by MTT.


RESULTS
The expression of beta1 integrin and cyclin D1 were decreased notably by inhibitors of N-linked oligosaccharide chains-TM. TM increased apoptosis and decreased the proliferative abilitiy of cells, and all the effects of TM were dose-dependent.


CONCLUSIONS
Through repressed glycosylation of glycoprotein, TM can suppress the expression of beta1, integrin, affect the adhesion of cells, and increase the apoptosis rate; at the same time, the expression of cyclin D1 and the proliferative ability of cells were decreased, and all these were dose-dependent.
Cai
Wen-li
WL
Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
Huang
Song-min
SM
Zeng
Li
L
Fu
Ping
P
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000096:biosynthesis; D006023:Glycoproteins / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000096:biosynthesis*; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D025942:Oligosaccharides, Branched-Chain / Q000494:pharmacology*; D051381:Rats
D006023:Glycoproteins; D019012:Integrin beta1; D025942:Oligosaccharides, Branched-Chain; D019938:Cyclin D1