[The protective effect of high density lipoprotein on the cardiac function of rats with severe burns].
OBJECTIVE
To investigate the protective effect of high density lipoprotein on the cardiac function of rats with severe burns.


METHODS
One hundred and thirty-five Wistar rats were employed in the study and were randomly divided into control (n = 15, without treatment), burn (n = 60, with 30% TBSA full-thickness burn on the back) and experimental (n = 60, with the injection of HDL (80 mg/kg) via the caudal vein immediately after burns) groups. The rats in the groups with burn injury were resuscitated with intraperitoneal isotonic saline (50 ml/kg) 30 minutes after burn (PBM). The serum contents of CK, ICAM-1 and TNF-alpha of the rats of all the three groups were determined with corresponding methods. The histological changes in the cardiac muscle tissue of the rats in all groups were observed under light microscope and electronic microscope.


RESULTS
The serum contents of CK, ICAM-1 and TNF-alpha in the control group were obviously lower than those in burn group (P < 0.01), while those in experimental group were also markedly lower than those in control group (P < 0.05 or 0.01). The average reduction rate was 36.5%, 32.0% and 12.6%, respectively. The size and the structure of the cardiac muscular fiber in the control group were even and normal. Compared with the burn group, degeneration, inflammatory infiltration and mitochondrial swelling were found to be less marked in the experimental group at 48 PBH, and no focal lysis and necrosis were found, which were observed in the burn group.


CONCLUSIONS
High density lipoprotein can be beneficial to the protection of cardiac tissue in protecting from secondary injury in rats with severe burns.
Department of Burns and Plastic Surgery, The 175th Hospital of PLA, Zhangzhou 363000, P.R. China.
Qing-yi
QY
Zheng
An-gen
AG
Hu
Shao-fu
SF
Cai
Jing-he
JH
Chen
Min-feng
MF
Zhang
Yang-gen
YG
Zhang
D000818:Animals; D002056:Burns / Q000097:blood* / Q000503:physiopathology*; D003402:Creatine Kinase / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D008075:Lipoproteins, HDL; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D003402:Creatine Kinase