Inhibitory effect of Weissella cibaria isolates on the production of volatile sulphur compounds.
OBJECTIVE
The objective of this study was to characterize the inhibitory effects of Weissella cibaria isolates on volatile sulphur compounds (VSC) production both in vitro and in vivo.


METHODS
We isolated and identified three hydrogen peroxide-generating lactobacilli from children's saliva, and assessed their inhibitory effects on VSC production and Fusobacterium nucleatum proliferation. Clinical studies were conducted with 46 subjects in order to measure the VSC of their mouth air.


RESULTS
These lactobacilli were identified as W. cibaria. These isolates inhibited the production of VSC by F. nucleatum (p<0.05). The concentration of F. nucleatum was decreased by 5-log cycles as a result of exposure to the W. cibaria strains (p<0.05), whereas the catalase-treated W. cibaria cultures exerted no evident inhibitory effects on F. nucleatum replication. In the clinical studies, gargling with one isolate resulted in a significant reduction in the levels of H2S and CH3SH by approximately 48.2% (p<0.01) and 59.4% (p<0.05), respectively.


CONCLUSIONS
These results indicate that W. cibaria isolates possess the ability to inhibit VSC production under both in vitro and in vivo conditions, demonstrating that they bear the potential for development into novel probiotics for use in the oral cavity.
Department of Microbiology and Immunology, School of Medicine, Chonnam National University, Gwangju, Korea.
Mi-Sun
MS
Kang
Byung-Gook
BG
Kim
Jin
J
Chung
Hyun-Chul
HC
Lee
Jong-Suk
JS
Oh
D000328:Adult; D000898:Antibiosis; D002374:Catalase / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D015169:Colony Count, Microbial; D005260:Female; D016967:Fusobacterium nucleatum / Q000187:drug effects / Q000254:growth & development; D006209:Halitosis / Q000517:prevention & control; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000032:analysis / Q000037:antagonists & inhibitors; D007778:Lactobacillus / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D008297:Male; D016877:Oxidants / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000254:growth & development; D018720:Prevotella / Q000187:drug effects / Q000254:growth & development; D019936:Probiotics; D012463:Saliva / Q000382:microbiology; D013438:Sulfhydryl Compounds / Q000032:analysis; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013457:Sulfur Compounds / Q000037:antagonists & inhibitors* / Q000378:metabolism; D048373:Treponema denticola / Q000187:drug effects / Q000254:growth & development
D016877:Oxidants; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D013457:Sulfur Compounds; D006861:Hydrogen Peroxide; D002374:Catalase; D006862:Hydrogen Sulfide