PCR detection of porcine circovirus type 2 (PCV2) DNA in blood, tonsillar and faecal swabs from experimentally infected pigs.
PCV2 infection is now recognized as the major factor in the development of post-weaning multisystemic wasting syndrome (PMWS). In this study we evaluated the use of PCR to detect the presence of PCV2 DNA in blood, faecal and tonsillar swabs collected from 12 pigs experimentally infected with PCV2 and sampled at selected time points post-infection. The PCR results were evaluated together with the presence of PMWS typical histopathological lesions and the presence of PCV2 antigen. PCV2 DNA was present in the blood of all 12 infected pigs at the end of the experiment and faecal and tonsillar swabs of 11 of the 12 pigs. The rate of PCR-positive serum and plasma samples was significantly higher in four pigs that showed virological and pathological evidence of PMWS, than in infected pigs without evidence of disease. In conclusion this study confirms that PCR cannot substitute for the traditional methods used for diagnosis of PMWS, however, PCR amplification of PCV2 DNA from serum or plasma could be a useful tool to support an early diagnosis of PMWS in live animals.
Caprioli
A
A
Department of Veterinary Public Health and Animal Pathology, Faculty of Veterinary Medicine, Bologna University, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, BO, Italy. acaprioli@vet.unibo.it
McNeilly
F
F
McNair
I
I
Lagan-Tregaskis
P
P
Ellis
J
J
Krakowka
S
S
McKillen
J
J
Ostanello
F
F
Allan
G
G
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D018173:Circoviridae Infections / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics*; D004279:DNA, Viral / Q000097:blood / Q000235:genetics / Q000378:metabolism; D005243:Feces / Q000821:virology; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006651:Histocytochemistry / Q000662:veterinary; D014066:Palatine Tonsil / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000821:virology*; D019282:Wasting Syndrome / Q000097:blood / Q000662:veterinary* / Q000821:virology
D000956:Antigens, Viral; D004279:DNA, Viral