Açai (Euterpe oleracea Mart.) polyphenolics in their glycoside and aglycone forms induce apoptosis of HL-60 leukemia cells.
The effects of açai polyphenolics on the antiproliferation and induction of apoptosis in HL-60 human leukemia cells were investigated. Interactions between anthocyanins and non-anthocyanin-polyphenolics in both their glycosidic and their aglycone forms were also investigated to determine additive or nonadditive responses. Polyphenolic fractions at 0.17-10.7 microM were found to reduce cell proliferation from 56 to 86% likely due to caspase-3 activation (apoptosis). Anthocyanin and polyphenolic fractions were nonadditive in their contribution to the cell antiproliferation activity. At equimolar concentrations, the glycosidic forms of phenolic acids and flavonoids induced a higher magnitude of change in cell parameters (proliferation and apoptosis) than their respective aglycone forms, while the opposite trend was observed for anthocyanin aglycones. This study demonstrated that açai offers a rich source of bioactive polyphenolics and confirmed the importance of investigating whole food systems when evaluating the potential health benefits of individual phytochemical compounds.
Del Pozo-Insfran
David
D
Department of Food Science and Human Nutrition, University of Florida, P.O. Box 110370, Gainesville, Florida 32611-0370, USA.
Percival
Susan S
SS
Talcott
Stephen T
ST
D000872:Anthocyanins / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D028023:Arecaceae / Q000737:chemistry*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004347:Drug Interactions; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D059808:Polyphenols
D000872:Anthocyanins; D005419:Flavonoids; D006027:Glycosides; D010636:Phenols; D059808:Polyphenols; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases