Substrate and metabolite diffusion within model medium for soft cheese in relation to growth of Penicillium camembertii.
Penicillium camembertii was cultivated on a jellified peptone-lactate based medium to simulate the composition of Camembert cheese. Diffusional limitations due to substrate consumption were not involved in the linear growth recorded during culture, while nitrogen (peptone) limitation accounted for growth cessation. Examination of gradients confirmed that medium neutralization was the consequence of lactate consumption and ammonium production. The diffusion of the lactate assimilated from the core to the rind and that of the ammonium produced from the rind to the core was described by means of a diffusion/reaction model involving a partial linking of consumption or production to growth. The model matched experimental data throughout growth.
Aldarf
Mazen
M
UMR CNRS 6226 "Sciences Chimiques de Rennes", ENSCR, Chimie et Ingénierie des Procédés-Université de Rennes 1/ENSCR, Campus de Beaulieu, Avenue du Général Leclerc, 35700, Rennes, France.
Fourcade
Florence
F
Amrane
Abdeltif
A
Prigent
Yves
Y
D002611:Cheese / Q000382:microbiology* / Q000592:standards; D003470:Culture Media / Q000378:metabolism; D004058:Diffusion; D005285:Fermentation; D005516:Food Microbiology; D019344:Lactic Acid / Q000378:metabolism; D010407:Penicillium / Q000254:growth & development* / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013379:Substrate Specificity
D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D019344:Lactic Acid