Multistrain genetic comparisons reveal CCR5 as a receptor involved in airway hyperresponsiveness.
Asthma is a ubiquitous disease with a broad range of clinical phenotypes. To better understand the complex genetic and environmental interactions underlying asthma, we compared the gene-gene interactions of four genetically distinct mouse strains that demonstrate biologically distinct responses to allergen. Using DNA microarrays and knock-out mouse studies, we showed that CCR5 plays a definitive role in the development of ovalbumin-induced allergic airway inflammatory disease. In addition, gene expression profiling data have revealed other potential novel targets for therapeutics-based research and has enhanced the understanding of the molecular mechanisms underlying the etiology of "asthma."
Department of Pulmonary, Allergy, and Critical Care Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA. walke082@mc.duke.edu
Julia K L
JK
Walker
Adriana
A
Ahumada
Bryan
B
Frank
Renee
R
Gaspard
Katherine
K
Berman
John
J
Quackenbush
David A
DA
Schwartz
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D018893:Bronchoalveolar Lavage; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D010047:Ovalbumin; D010641:Phenotype; D019713:Receptors, CCR5 / Q000235:genetics* / Q000378:metabolism*; D012130:Respiratory Hypersensitivity / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism*
D019713:Receptors, CCR5; D010047:Ovalbumin