Effects of codon modification on human BMP2 gene expression in tobacco plants.
Bone morphogenetic protein 2 (BMP2) has great potential in therapeutic applications. We are working on generating transgenic plants as a bioreactor to produce BMP2. We have studied the effects of codon optimization on the expression of human BMP2 (hBMP2) in tobacco plants. Three modified hBMP2 genes were transformed into tobacco under the control of either cauliflower mosaic virus 35S (CaMV35S) promoter or double-CaMV35S promoter plus alfalfa mosaic virus (AMV) enhancer. The fused beta-glucuronidase (GUS) reporter gene was used to facilitate the assay of protein expression. The results indicated that codon optimization could increase the protein expression level obviously under CaMV35S promoter. However, under relatively stronger initiation condition (double-CaMV35S promoter plus AMV enhancer), only the gene with the lowest degree of codon optimization could increase the protein expression level. Our findings suggest that the action of codon optimization may be influenced by the factors of promoter strength and A+T content in tobacco plants.
Suo
Guangli
G
Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology and the Graduate School, Chinese Academy of Sciences, Beijing, 100080, People's Republic of China.
Chen
Bing
B
Zhang
Jingyu
J
Duan
Ziyuan
Z
He
Zhengquan
Z
Yao
Wei
W
Yue
Chaoyin
C
Dai
Jianwu
J
D001483:Base Sequence; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000378:metabolism*; D003062:Codon / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism*
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D003062:Codon; D016212:Transforming Growth Factor beta