Physicochemical modification of the excretion product of Saccharomyces cerevisiae killer strains results in fungicidal activity against Candida albicans and Tricophyton mentagrophytes.
It is known that certain yeast strains, so called 'killers', can produce and excrete proteinaceous toxins that can induce death of other sensitive strains. We obtained a stable fungicidal factor (SKF) through concentration and stabilization of the excretion product of certain killer strains of Saccharomyces cerevisiae (K1 and K2). The isolated proteinaceous complex exhibited activity at broad ranges of pH (4-7.5) and temperatures (20-37.5 degrees C). It was significantly lethal against Candida albicans and Tricophyton mentagrophytes. SKF showed stability and activity after storage, with a mean half-life of 6 months at 4 degrees C or at -20 degrees C.
Bracesco
Nelson
N
Lab. Radiobiología. Dpt. Biofísica. Facultades de Medicina y Ciencias, Univ. de la República Uruguay. nbracesco@fmed.edu.uy
Salvo
Virgilio A
VA
Carrau
Francisco M
FM
Nunes
Elia
E
D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects*; D004356:Drug Storage / Q000592:standards; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D016019:Survival Analysis; D013997:Time Factors; D014249:Trichophyton / Q000187:drug effects*
D000935:Antifungal Agents; D029701:Saccharomyces cerevisiae Proteins