The RACK1 homologue from Trypanosoma brucei is required for the onset and progression of cytokinesis.
The receptor for activated C kinase 1 (RACK1) is a conserved scaffold protein that helps regulate a range of cell activities including cell growth, shape, and protein translation. We report that a homologue of RACK1 is required for cytokinesis in pathogenic Trypanosoma brucei. The protein, referred to as TRACK, is comprised of WD repeat elements and can complement cpc2 null mutants of Schizosaccharomyces pombe. TRACK is expressed throughout the trypanosome life cycle and is distributed predominantly in a perinuclear region and the cytoplasm but not along the endoplasmic reticulum, mitochondrion, or cleavage furrow of dividing cells. When tetracycline-inducible RNA interference (RNAi) is used to deplete the cellular content of TRACK, the cells remain metabolically active, but growth is inhibited. In bloodstream forms, growth arrest is due to a delay in the onset of cytokinesis. By contrast, procyclic forms are able to initiate cytokinesis in the absence of TRACK but arrest midway through cell cleavage. The RNAi cells undergo multiple rounds of partial cytokinesis and accumulate nuclei and cytoplasmic extensions with attached flagella. The TRACK RNAi construct is also inducible within infected mice. Under these conditions parasites are eliminated from peripheral blood within 3 days post-infection. Taken as a whole, these data indicate that trypanosomes utilize a RACK1 homologue to regulate the final stages of mitosis. Moreover, disrupting the interaction between TRACK and its partners might be targeted in the design of novel therapies.
Department of Biological Sciences, Southern Methodist University, Dallas, Texas 75275, USA.
Karen G
KG
Rothberg
Dara L
DL
Burdette
Joy
J
Pfannstiel
Neal
N
Jetton
Rashmi
R
Singh
Larry
L
Ruben
D000818:Animals; D001681:Biological Assay; D002455:Cell Division; D048749:Cytokinesis / Q000502:physiology*; D003593:Cytoplasm / Q000737:chemistry; D004721:Endoplasmic Reticulum / Q000502:physiology; D020869:Gene Expression Profiling; D051379:Mice; D034622:RNA Interference; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000502:physiology*; D012568:Schizosaccharomyces / Q000235:genetics / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*
D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D029702:Schizosaccharomyces pombe Proteins; C000617313:cpc2 protein, S pombe