Mammalian peptidoglycan recognition protein TagL inhibits Listeria monocytogenes invasion into epithelial cells.
Peptidoglycan recognition proteins are a family of evolutionary conserved proteins that play a basic role in the innate immunity of insects, but their role in the immunity of mammals remains unclear. To elucidate its functions, a mouse member of the peptidoglycan recognition proteins family, TagL, was stably expressed in colon adenocarcinoma HT29 cells, and its effect on the invasion and intracellular growth of the enteroinvasive pathogenic bacterium Listeria monocytogenes was assessed. The expression of TagL substantially impaired bacterial invasion and early intracellular growth. The observed effects were partly caused by a loss of viability by intraphagosomal bacteria. Efficient phagosome escaping but not efficient invasion helped bacteria to overplay TagL.
Kibardin
Alexey
A
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Karpova
Tatyana
T
Sapenko
Tatyana
T
Vazquez-Boland
Jose Antonio
JA
Kiselev
Sergey
S
Ermolaeva
Svetlana
S
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D019073:HT29 Cells; D006801:Humans; D008089:Listeria monocytogenes / Q000254:growth & development / Q000472:pathogenicity*; D051379:Mice; D010588:Phagosomes / Q000382:microbiology; D014162:Transfection
D002352:Carrier Proteins; C118897:peptidoglycan recognition protein