The spatial relationship between the musculature and the NADPH-diaphorase activity, 5-HT and FMRFamide immunoreactivities in redia, cercaria and adult Echinoparyphium aconiatum (Digenea).
The spatial relationship between the musculature and the NADPH-diaphorase (NADPH-d) activity, 5-HT and FMRFamide immunoreactivities in redia, cercaria and adult Echinoparyphium aconiatum was studied using scanning electron microscopy (SEM), NADPH-d histochemistry, immunocytochemistry, and confocal scanning laser microscopy (CSLM). TRITC-conjugated phalloidin was used to stain the musculature. Staining for NADPH-d was observed in the central (CNS) and peripheral nervous system (PNS) of all three stages. NADPH-d positive nerves occurred very close to muscle fibres. 5-HT-immunoreactive (5-HT-IR) nerve cells and fibres occurred in the CNS and PNS and close to muscle fibres. FMRFamide-IR nerve fibres were observed in the CNS and PNS of adult worms. This is the first time, the presence of the NADPH-d has been demonstrated in the larval as well as the adult stages of a fluke.
Institute of Parasitology, Russian Academy of Sciences, Lenin Avenue 33, 117071 Moscow, Russia.
N B
NB
Terenina
O
O
Tolstenkov
H-P
HP
Fagerholm
E A
EA
Serbina
S N
SN
Vodjanitskaja
M K S
MK
Gustafsson
D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure; D019835:FMRFamide / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D007150:Immunohistochemistry; D007814:Larva / Q000648:ultrastructure; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009252:NADPH Dehydrogenase / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D026602:Nitrergic Neurons / Q000648:ultrastructure; D012701:Serotonin / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D014200:Trematoda / Q000648:ultrastructure*
D012701:Serotonin; D019835:FMRFamide; D009252:NADPH Dehydrogenase