Membrane-activated form of glycoproteins IIb/IIIa complex on newborn platelets.
BACKGROUND
GPIIb/IIIa complex is one of the laboratory markers of platelet activation. Closely associated with this process is exposure of the fibrinogen-binding site which is essential for platelets aggregation. This study was performed to evaluate the expression of GPIIb/IIIa on the platelets at time of birth.


METHODS
51 term newborns, 21 females and 30 males, were introduced to the study. They fitted all criteria for healthy term newborns. Blood was collected from umbilical artery and vein immediately after cutting the umbilical cord using Diatube TM CTAD Vacutainer System. GPIIb/IIIa was tested with PAC-1-FITC antibody using flow cytometer EPICS XL.


RESULTS
90.91% of newborn platelets express GPIIb/IIIa complex in comparison to adults, where only 12.79% platelets express this complex. Practically no differences were noted in relation to gender of newborns and umbilical artery or vein.


CONCLUSIONS
Careful analysis of the presented data reveals that at time of birth platelets are hyperreactive, which may be related to the increased thrombogenesis in newborns and mothers.
Department of Neonatology and Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, Bialystok, Poland. awasiluk@amb.edu.pl
Alicja
A
Wasiluk
D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007231:Infant, Newborn / Q000097:blood*; D008297:Male; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism*; D012737:Sex Factors; D014469:Umbilical Arteries; D014471:Umbilical Veins
D019039:Platelet Glycoprotein GPIIb-IIIa Complex