Study of the efficacy of Coated VICRYL Plus Antibacterial suture (coated Polyglactin 910 suture with Triclosan) in two animal models of general surgery.
OBJECTIVE
To evaluate the efficacy in vitro and in vivo of a new antibacterial suture (PGAB) compared with a traditional braided suture (PG). Our primary goals were to study microbiological effectiveness and impact on wound healing of PGAB vs PG. Secondary goal was to analyze influence on inflammatory response.


METHODS
In vitro study: clinical samples of Staphylococcus epidermidis, Staphylococcus aureus, S. hominis, Staphylococcus haemolyticus, Staphylococcus auricularis, Enterococcus faecalis, Corynebacterium spp. and Escherichia coli were studied. We also implanted a flat mesh in 10 minipigs, four incisions each (two PG and two PGAB) two contaminated with S. epidermidis and two not contaminated. Finally, we performed four colic anastomosis in each of 10 minipigs, two contaminated with E. coli and two not contaminated (two PG and two PGAB). We studied the inflammatory and wound healing processes in both models.


RESULTS
We observed a bactericidal efficacy of PGAB against grampositive, and bacteriostatic effect against E. coli. Mesh study: recovered CFU were lower in the group PGAB vs PG. In the group PGAB, inflammatory mediators' concentrations were lower. In the group PGAB, concentrations of wound healing mediators were normal. Colic anastomosis: recovered CFU were lower in the group PGAB vs the group PG. In the group PGAB we observed a reduction of inflammatory mediators. In the group PGAB we observed normalized concentrations of wound healing mediators.


CONCLUSIONS
This study demonstrates microbiological efficacy of PGAB, that normalizes wound healing process, and an anti-inflammatory effect.
Department of Surgery, Experimental Research Center (CBIE), Medical School, University of Salamanca, Po San Vicente 108-132, Salamanca 37007, Spain. jlama@fonendo.com
A
A
Gómez-Alonso
F J
FJ
García-Criado
F C
FC
Parreño-Manchado
J E
JE
García-Sánchez
E
E
García-Sánchez
A
A
Parreño-Manchado
Y
Y
Zambrano-Cuadrado
D000714:Anastomosis, Surgical; D000818:Animals; D000891:Anti-Infective Agents, Local; D015703:Antigens, CD / Q000096:biosynthesis; D001424:Bacterial Infections / Q000517:prevention & control*; D015169:Colony Count, Microbial; D006909:Hydroxyproline / Q000096:biosynthesis; D023421:Models, Animal; D016328:NF-kappa B / Q000032:analysis; D052247:Nitric Oxide Synthase Type II / Q000032:analysis; D009195:Peroxidase / Q000032:analysis; D011098:Polyglactin 910; D013481:Superoxides / Q000032:analysis; D013530:Surgical Wound Infection / Q000517:prevention & control*; D013536:Suture Techniques; D013537:Sutures / Q000382:microbiology*; D013552:Swine; D013556:Swine, Miniature; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D014260:Triclosan; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D014945:Wound Healing
D000891:Anti-Infective Agents, Local; D015703:Antigens, CD; D016328:NF-kappa B; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides; D011098:Polyglactin 910; D014260:Triclosan; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; D006909:Hydroxyproline