Isolation and characterization of a pure mannan from Oncidium (cv. Gower Ramsey) current pseudobulb during initial inflorescence development.
A water-soluble and neutral polysaccharide was extracted from the current pseudobulbs of Oncidium "Gower Ramsey" during the early inflorescence stage (flower stalk less than 4 cm) by hot water, precipitated with ethanol, and purified with an anion exchanger. From the data of monosaccharide composition and linkage and anomeric configuration analyses, the polysaccharide was identified as a linear beta-1-->4 linked mannan.
Department of Life Sciences, National Kaohsiung University, Taiwan. hlwang@nuk.edu.tw
Heng-Long
HL
Wang
Kai-Wun
KW
Yeh
Peng-Ren
PR
Chen
Chih-Hao
CH
Chang
Jin-Mei
JM
Chen
Kay-Hooi
KH
Khoo
D002855:Chromatography, Thin Layer; D035264:Flowers / Q000254:growth & development*; D008351:Mannans / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D029595:Orchidaceae / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D012995:Solubility; D013997:Time Factors; D014867:Water
D008351:Mannans; D014867:Water