In vitro storage of cedar shoot cultures under minimal growth conditions.
We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were grown on a diluted modified MS medium. The addition of 6% mannitol to the storage media affected negatively survival and multiplication capacity of the cultures. The slow-growth storage conditions used in our experiments did not induce remarkable effects on both RAPD variability and average DNA methylation in the species.
Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100-Burjasot, Valencia, Spain.
Begoña
B
Renau-Morata
Isabel
I
Arrillaga
Juan
J
Segura
D028203:Cedrus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D003470:Culture Media / Q000494:pharmacology; D019175:DNA Methylation / Q000187:drug effects; D010802:Phylogeny; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development*; D019105:Random Amplified Polymorphic DNA Technique; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media