[Inducing apoptosis of HeLa cell lines by transfection of c-myc antisense oligonucleotides].
OBJECTIVE
To investigate the effect on apoptosis of HeLa cells by transfecting c-myc antisense oligonucleotides and seek a new method to enhance radiosensitivity of tumor cells.


METHODS
Immunohistochemistry staining and RT-PCR were conducted to identify the effect of ASODN. Giemsa staining, flow cytometry (FCM) and DNA ladder were performed to detect the apoptosis of HeLa cells.


RESULTS
After transfection of ASODN (c-myc), the levels of both c-myc mRNA and C-MYC protein expression were reduced (P < 0.05). Giemsa staining demonstrated apoptosis-bodies on the c-myc ASODN-transfected cells. DNA ladder presented the typical ladder of apoptosis. The apoptosis rate of HeLa cells transfected with c-myc ASODN was (10.29 +/- 0.66)%, transfected c-myc ASODN combined irradiation was (16.83 +/- 0.57)%, which were much higher than that of control group (1.79 +/- 0.19)% (P < 0.05).


CONCLUSIONS
The c-myc ASODN could effectively down-regulate the expression of c-myc; furthermore, transfection of c-myc ASODN could more effectively induce apoptosis of HeLa cells which subsequently enhanced radiosensitivity of HeLa cells.
Department of Radiotherapy, Qilu Hospital, Shandong University, Jinan 250012, China.
Rong-rong
RR
Li
Dao-Xin
DX
Ma
Yu-feng
YF
Cheng
D017209:Apoptosis / Q000235:genetics* / Q000528:radiation effects; D005260:Female; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D016376:Oligonucleotides, Antisense; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger