[Screening of phytase-producing strain and its optimal solid state phytase-producing conditions].
Phytase can catalyze phytate hydrolyzed into inositol and phosphoric acid, and its application could make the phytic acid in animal feeds become more available, decrease feed cost, and at the same time, protect eco-environment. In this paper, a phytase-producing strain Trichoderma Viride LH374 was obtained through isolation and mutation, and its optimal conditions for solid state fermentation and phytase production were investigated. The results suggested that the optimal conditions of solid state fermentation were straw:rice bran ratio 8:2, medium initial pH 6.5, temperature 30 degrees C, culture duration 96 h, and moisture content 60%. Under the optimal conditions, LH374 could accumulate phytase up to 1,580 U x g(-1).
Fujian Agriculture and Forestry University, Fuzhou, China. wshyyl@sina.com
Shihua
S
Wang
Kaihui
K
Hu
Wenxiong
W
Lin
D010832:6-Phytase / Q000096:biosynthesis*; D014242:Trichoderma / Q000201:enzymology* / Q000302:isolation & purification*
D010832:6-Phytase