Less common applications of monoliths: I. Microscale protein mapping with proteolytic enzymes immobilized on monolithic supports.
This review summarizes the recent contributions to the rapidly growing area of immobilized enzymes employing both silica and synthetic polymer-based monoliths as supports. Focus is mainly on immobilized proteolytic enzyme reactors designed for studies in proteomics. Porous monoliths emerged first as a new class of stationary phases for HPLC in the early 1990s. Soon thereafter, they were also used as supports for immobilization of proteins and preparation of both stationary phases for bioaffinity chromatography and enzymatic reactors. Organic polymer-based monoliths are typically prepared using a simple molding process carried out within the confines of a "mold" such as chromatographic column or capillary. Polymerization of a mixture comprising monomers, initiator, and porogenic solvent affords macroporous materials. In contrast, silica-based monoliths are first formed as a rigid rod from tetraalkoxysilane in the presence of PEG and subsequently encased with a plastic tube. Both types of monolith feature large through-pores that enable a rapid flow-through. Since all the solutions must flow through the monolith, the convection considerably accelerates mass transfer within the monolith. As a result, reactors including enzyme immobilized on monolithic support exhibit much higher activity compared to the reactions in solution.
Svec
Frantisek
F
Department of Chemistry, University of California, Berkeley, CA, USA. svec@berkeley.edu
D020106:Acrylamide; D000447:Aldehydes / Q000737:chemistry; D000588:Amines / Q000737:chemistry; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D005782:Gels; D006868:Hydrolysis; D008689:Methacrylates; D010084:Oxidation-Reduction; D010447:Peptide Hydrolases; D010449:Peptide Mapping / Q000379:methods*; D010777:Photochemistry; D011108:Polymers; D012822:Silicon Dioxide
D000447:Aldehydes; D000588:Amines; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D005782:Gels; D008689:Methacrylates; D011108:Polymers; D020106:Acrylamide; D012822:Silicon Dioxide; D010447:Peptide Hydrolases; C007870:glycidyl methacrylate