Renal nerves participation in the effects of nitric oxide and ET(A)/ET(B) receptor inhibition in spontaneously hypertensive rats.
The influence of renal nerves on the effects of concurrent NO synthase inhibition (10 mg kg(-1) b.w. i.v. L-NAME) and ET(A)/ET(B) receptor inhibition (10 mg kg(-1) b.w. i.v. bosentan) on renal excretory function and blood pressure in conscious spontaneously hypertensive rats (SHR) was investigated. L-NAME increased blood pressure, urine flow rate, fractional excretion of sodium, chloride and phosphate in both normotensive Wistar rats and SHR with intact renal nerves (p<0.01). GFR or RBF did not change in any of the groups investigated. The effects of L-NAME on renal excretory function were markedly reduced by bosentan and the values returned to control level in the normotensive rats, while in SHR the values were reduced by bosentan, but they remained significantly elevated as compared to control level (p<0.05). The hypertensive response induced by L-NAME in SHR is partially due to activation of endogenous endothelins, but it does not depend on renal nerves. Chronic bilateral renal denervation abolished the effect of L-NAME on sodium and chloride excretion in normotensive rats, whereas it did not alter this effect in SHR. The participation of endogenous endothelins in changes of renal excretory function following NO synthase inhibition is diminished in SHR as compared to Wistar rats.
Department of Physiology, Medical University, Sofia, Bulgaria. girchev@medfac.acad.bg
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D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D003714:Denervation; D065130:Endothelin A Receptor Antagonists; D065131:Endothelin B Receptor Antagonists; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007668:Kidney / Q000294:innervation* / Q000502:physiology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010710:Phosphates / Q000652:urine; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D012964:Sodium / Q000652:urine; D013564:Sympathetic Nervous System / Q000502:physiology; D014563:Urodynamics / Q000187:drug effects / Q000502:physiology
D065130:Endothelin A Receptor Antagonists; D065131:Endothelin B Receptor Antagonists; D004791:Enzyme Inhibitors; D010710:Phosphates; D009569:Nitric Oxide; D012964:Sodium; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester