[Rational evolutionary design and modification of gene: a short-path of direct evolution].
Directed evolution is a powerful means in academic study and industrial application to modified and functionally improved proteins. There have been significant and impressive advances using the artificial method. Rational Evolutionary Design utilizes structural and sequence alignment information to create new genes and proteins. Rational Evolutionary Design has recently emerged as an attractive approach for studying function of proteins. The review mainly introduced four aspects: preferred codon usage, structural and sequence alignment, key domain analysis, site-directed recombination.
Xiong
Ai-Sheng
AS
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. aishengxiong@yahoo.com
Yao
Quan-Hong
QH
Zhang
Zhen
Z
Peng
Ri-He
RH
Zhuang
Jing
J
Xu
Fang
F
Liu
Jin-Ge
JG
Zhu
Hong
H
D019020:Directed Molecular Evolution; D019143:Evolution, Molecular; D005796:Genes / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D016415:Sequence Alignment