Noninvasive myocardial strain measurement by speckle tracking echocardiography: validation against sonomicrometry and tagged magnetic resonance imaging.
OBJECTIVE
The aim of this study was to validate speckle tracking echocardiography (STE) as a method for angle-independent measurement of regional myocardial strain, using sonomicrometry and magnetic resonance imaging (MRI) tagging as reference methods.


BACKGROUND
Tissue Doppler imaging allows non-invasive measurement of myocardial strain in the left ventricle (LV), but is limited by angle dependency.


METHODS
Strain measurements with STE were obtained by a custom-made program that allowed tracking of two-dimensional motion of speckle patterns in a B-mode image. In anesthetized dogs, we compared LV long- and short-axis measurements by STE to sonomicrometry during preload changes and regional myocardial ischemia. Measurements in the two orthogonal axes were obtained simultaneously in a single imaging plane. In human subjects, long-axis strain by STE and MRI tagging were compared in multiple segments of the LV.


RESULTS
In the experimental study there was good correlation and agreement between STE and sonomicrometry for systolic strain in the long axis (r = 0.90, p < 0.001; 95% limits of agreement -4.4% to 5.0%) and systolic shortening in the short axis (r = 0.79, p < 0.001; -5.6% to 5.1%). In the clinical study, 80% of the segments could be analyzed, and correlation and agreement between STE and MRI tagging were good (r = 0.87, p < 0.001; -9.1% to 8.0%).


CONCLUSIONS
Speckle tracking echocardiography provides accurate and angle-independent measurements of LV dimensions and strains and has potential to become a clinical bedside tool for quantifying myocardial strain.
Amundsen
Brage H
BH
Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway. brage.h.amundsen@ntnu.no
Helle-Valle
Thomas
T
Edvardsen
Thor
T
Torp
Hans
H
Crosby
Jonas
J
Lyseggen
Erik
E
Støylen
Asbjørn
A
Ihlen
Halfdan
H
Lima
João A C
JA
Smiseth
Otto A
OA
Slørdahl
Stig A
SA
D000328:Adult; D000368:Aged; D000818:Animals; D004285:Dogs; D004452:Echocardiography / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods; D009200:Myocardial Contraction; D016277:Ventricular Function, Left