[Role of mitochondria in Tubeimoside I-mediated apoptosis in human cervical carcinoma HeLa cell line].
OBJECTIVE
To investigate the role of mitochondria in TBMS1-medited apoptosis of human cervical carcinoma HeLa cell line.


METHODS
Mitochondrial transmembrane potentia (deltapsim) was assayed by flow cytometry. Apoptotic induction by TBMS1 was determined by gel electrophoresis of fragmented DNA. Cytochrome c (Cyt c) was detected by Western blotting.


RESULTS
The results showed that TBMS1 induced apoptosis in HeLa cells, that TBMS1 decreased deltapsim and facilitated Cyt c release, and that TBMS1 induced apoptosis of HeLa cells dose-and time-dependently in accordance with increase of cytosolic Cyt c. TBMS1 had direct effect on isolated rat mitochondria which induced a time- and dose-dependent release of Cyt c from mitochondria. The results also showed that CsA protected partly HeLa cells from the effect of TBMS1.


CONCLUSIONS
The mitochondrial apoptosis pathway has effects on TBMS1 induced human cervical carcinoma HeLa cell line apoptosis.
South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Fang
F
Wang
Run-Di
RD
Ma
Li-Jian
LJ
Yu
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D019662:Cucurbitaceae / Q000737:chemistry; D016572:Cyclosporine / Q000494:pharmacology; D045304:Cytochromes c / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000378:metabolism / Q000502:physiology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*
D012503:Saponins; D014315:Triterpenes; C051084:tubeimoside I; D016572:Cyclosporine; D045304:Cytochromes c