Comparison of the Paul ion trap to the linear ion trap for use in global proteomics.
A critical evaluation of the performance of a 2-D linear ion trap (IT) instrument to two 3-D quadrupole IT instruments with emphasis on identification of rat serum proteins by bottom-up LC-MS/MS is presented. The speed and sensitivity of each of the instruments were investigated, and the effects that each of these have on the bottom-up proteomics identification approach are discussed.
Riter
Leah S
LS
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA.
Gooding
Karen M
KM
Hodge
Barry D
BD
Julian
Randall K
RK
D000818:Animals; D001798:Blood Proteins / Q000032:analysis* / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D020543:Proteome; D040901:Proteomics; D051381:Rats; D012680:Sensitivity and Specificity
D001798:Blood Proteins; D020543:Proteome