Improved production of biosurfactant by a Pseudomonas aeruginosa mutant using vegetable oil refinery wastes.
Biosurfactant production by Pseudomonas aeruginosa EBN-8 mutant was studied in shake flasks on separate wastes from canola, soybean and corn oil refineries. Of the substrates tested, canola oil refinery waste (COD=20 g l(-1)) supplemented with sodium nitrate (at COD/N=20) showed the best microbial growth (4.50 g l(-1)) and rhamnolipid production (8.50 g l(-1)), at 10 d of incubation with the specific growth rate of 0.316 h(-1) and specific product yield of 0.597 g g(-1) h. Its cell-free supernatant showed the critical micelle dilution (CMD) of 150 and surface tension (ST) of 28.5 mN m(-1).
Polymer Chemistry Laboratory, National Centre of Excellence in Physical Chemistry, University of Peshawar, 25210 Peshawar, Pakistan.
Zulfiqar Ali
ZA
Raza
Asma
A
Rehman
Muhammad Saleem
MS
Khan
Zafar M
ZM
Khalid
D001673:Biodegradation, Environmental; D018533:Biomass; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism; D006017:Glycolipids / Q000378:metabolism; D007220:Industrial Waste / Q000032:analysis*; D009154:Mutation; D009566:Nitrates / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism*; D000074262:Rapeseed Oil; D013500:Surface Tension; D013501:Surface-Active Agents / Q000378:metabolism*
D005229:Fatty Acids, Monounsaturated; D006017:Glycolipids; D007220:Industrial Waste; D009566:Nitrates; D010938:Plant Oils; D000074262:Rapeseed Oil; D013501:Surface-Active Agents; C418382:rhamnolipid; C031618:sodium nitrate