A small HSP, Lo18, interacts with the cell membrane and modulates lipid physical state under heat shock conditions in a lactic acid bacterium.
The small heat shock proteins (sHSP) are characterized by a chaperone activity to prevent irreversible protein denaturation. This study deals with the sHSP Lo18 induced by multiple stresses in Oenococcus oeni, a lactic acid bacterium. Using in situ immunocytochemistry and cellular fractionation experiments, we demonstrated the association of Lo18 with the membrane in O. oeni cells submitted to heat shock. The same result was obtained after exposure of cells to ethanol or benzyl alcohol, agents known to have an influence on membranes. For the different stresses, the protein was located on the periphery of the cell at membrane level and was also found within the cytoplasm. In order to determine if Lo18 could interact with the phospholipids, we used model membranes made of lipids extracted from O. oeni cells. Using fluorescence anisotropy of diphenylhexatriene (DPH) and generalized polarization of Laurdan, we showed that purified Lo18 interacts with these liposomes, and increases the molecular order of the lipid bilayer in these membranes when the temperature reaches 33.8 degrees C. All these data suggest that Lo18 could be involved in an adaptive response allowing the maintenance of membrane integrity during stress conditions in O. oeni cells.
Laboratoire de Microbiologie UMR UB/INRA 1232, Université de Bourgogne, ENSBANA, 1, Esplanade Erasme, 21 000 Dijon, France.
Françoise
F
Coucheney
Laurent
L
Gal
Laurent
L
Beney
Jeannine
J
Lherminier
Patrick
P
Gervais
Jean
J
Guzzo
D002462:Cell Membrane / Q000378:metabolism*; D005454:Fluorescence Polarization; D006095:Gram-Positive Cocci / Q000378:metabolism*; D050888:Heat-Shock Proteins, Small / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D006358:Hot Temperature; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000737:chemistry*
D050888:Heat-Shock Proteins, Small; D008563:Membrane Lipids