Comparative proteomics and immunoproteomics of Helicobacter pylori related to different gastric pathologies.
Helicobacter pylori is a Gram-negative bacterium which causes ulcer, atrophic gastritis, adenocarcinoma, or mucosa-associated lymphoid tissue lymphoma. A comparative proteomic and immunoproteomic analysis was chosen to identify the antigenic patterns of three different H. pylori strains. These strains were probed against single sera from H. pylori-positive patients affected by gastric adenocarcinoma or duodenal ulcer. We found a quite heterogeneous antigenic pattern, both from strain and sera points of view, thus underlying both a strain- and a host-specificity. The different antigenic repertoires introduced the importance of the strain to be used for immunoblotting as a diagnostic test.
Dipartimento di Biologia Molecolare, Università degli Studi di Siena, via Fiorentina 1, 53100 Siena, Italy.
Roberta
R
Mini
Giulia
G
Bernardini
Anna Maria
AM
Salzano
Giovanni
G
Renzone
Andrea
A
Scaloni
Natale
N
Figura
Annalisa
A
Santucci
D001426:Bacterial Proteins / Q000032:analysis*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D016480:Helicobacter pylori / Q000737:chemistry* / Q000472:pathogenicity; D006801:Humans; D040901:Proteomics; D013272:Stomach Diseases / Q000382:microbiology*; D013274:Stomach Neoplasms / Q000382:microbiology*
D001426:Bacterial Proteins