Bifunctional effect of resveratrol on the expression of ErbB2 in human breast cancer cell.
This study evaluated the effect of resveratrol on the expression of ErbB2 in a human breast cancer cell line, MCF-7. Low concentrations of resveratrol (1-10microM) reduced the basal expression level of ErbB2 in MCF-7 cells cultured in an estrogen-free medium. When cells were cultured in a medium containing estrogen, resveratrol increased the ErbB2 protein levels in a dose-dependent manner. Resveratrol increased the luciferase reporter gene activity in cells transfected with the -756bp flanking region of the human erbB2 gene. Resveratrol increased the nuclear levels of AP-2alpha and AP-2gamma, and the induction of the luciferase reporter gene by resveratrol was inhibited by a mutation of two AP-2 binding sites in the promoter region of the human erbB2 gene. Blocking the ERK, p38 kinase or PI3-kinase activity had no effect on the resveratrol-inducible transactivation of the erbB2 gene and the ErbB2 expression level.
Research Center for Resistant Cells, Chosun University, Gwangju 501-759, South Korea.
Hoo Kyoon
HK
Choi
Jin Won
JW
Yang
Keon Wook
KW
Kang
D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D009154:Mutation; D011401:Promoter Regions, Genetic; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*; D017978:Receptors, Growth Factor / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*; D050656:Transcription Factor AP-2 / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D018719:Erb-b2 Receptor Tyrosine Kinases; D017978:Receptors, Growth Factor; D000077185:Resveratrol; D013267:Stilbenes; D050656:Transcription Factor AP-2; D048051:p38 Mitogen-Activated Protein Kinases