Behavior of human osteoblast-like cells in contact with electrodeposited calcium phosphate coatings.
Calcium-deficient hydroxyapatite (Ca-def-HAP) thin films were elaborated on Ti6Al4V substrates by electrodeposition. The coatings exhibit two different morphologies and crystallinities. Human osteoblast-like cells (MG-63) were cultured on the surfaces of these materials; the cell content and viability were evaluated up to 28 days. The scanning electron microscopy and biological investigations showed cells with a normal morphology, good proliferation, and viability from 7 to 21 days. But after 28 days, the number of live cells decreases in both cases; however, this decrease is less important in the case of calcium phosphate (CaP) coating surface when compared with the control (cell culture plastic). The cells cultured on Ca-def-HAP coating exhibit more cellular extensions and extracellular matrix. RT-PCR for type I collagen, alkaline phosphatase, and osteocalcin studies were also carried out, and was found that the CaP enhances gene expression of ALP and OC and thus the differentiation of osteoblast-like cells. Moreover, this study shows that the difference in the morphology of CaP coatings has no effect on the biocompatibility.
INSERM ERM 0203, 1 rue Maréchal Juin 51096 Reims Cedex, France.
D
D
Richard
N
N
Dumelié
H
H
Benhayoune
S
S
Bouthors
C
C
Guillaume
N
N
Lalun
G
G
Balossier
D
D
Laurent-Maquin
D002130:Calcium Phosphates / Q000737:chemistry; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004595:Electroplating; D006801:Humans; D010006:Osteoblasts / Q000502:physiology*
D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; C020243:calcium phosphate