Hyper-Raman microspectroscopy: a new approach to completing vibrational spectral and imaging information under a microscope.
We have developed hyper-Raman scattering microspectroscopy and applied it to a microcrystal of all-trans-beta-carotene. The hyper-Raman spectrum of all-trans-beta-carotene exhibits a Raman-inactive but infrared-active vibrational mode at 1564 cm(-1). Hyper-Raman imaging of a microcrystal was performed with this band. Infrared-active vibrational imaging was achieved with a spatial resolution much higher than that of conventional infrared microscopy. The combination of Raman and hyper-Raman spectroscopy opens up a new scope for high-spatial-resolution vibrational microspectroscopy that is not restricted by the selection rule.
Department of Chemistry, School of Science, The University of Tokyo, Japan.
Rintaro
R
Shimada
Hideaki
H
Kano
Hiro-o
HO
Hamaguchi
D003461:Crystallography / Q000379:methods*; D007089:Image Enhancement / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods; D014732:Vibration; D019207:beta Carotene / Q000032:analysis*
D019207:beta Carotene