[Study of rat's p53 gene damage and organ specificity induced by benzidine].
OBJECTIVE
Studying the main target organs and the genetic toxicity mechanism of benzidine.


METHODS
SD rats were given benzidine i.p. injection. DNA was extracted from rat's liver, kidney, lung and bladders. Then ss probes RDPCR was used to detect the damaged DNA of exon 7 of p53 gene.


RESULTS
Hybridization bands were found in liver, bladder and lung tissues after ss probes RDPCR, while no hybridization bands were found in kidney tissues.


CONCLUSIONS
The result indicates that benzidine can cause the DNA lesions of exon 7 of p53 gene and its major target organs are liver, bladder and lung. The toxicity mechanism of benzidine is probably related to p53 gene damage.
Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Qing
Q
Wu
Zheng-chang
ZC
Heng
D000818:Animals; D001560:Benzidines / Q000633:toxicity*; D004249:DNA Damage; D005091:Exons / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009154:Mutation / Q000187:drug effects; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism
D001560:Benzidines; C029876:benzidine