Antimicrobial activity of lysostaphin and a Listeria monocytogenes bacteriophage endolysin produced and secreted by lactic acid bacteria.
The expression and secretion signals of the Sep protein from Lactobacillus fermentum BR11 were used to direct export of two peptidoglycan hydrolases by Lb. fermentum BR11, Lactobacillus rhamnosus GG, Lactobacillus plantarum ATCC 14917 and Lactococcus lactis MG1363. The production levels, hydrolytic and bacteriocidal activities of the Listeria monocytogenes bacteriophage N-acetylmuramoyl-l-alanine amidase endolysin Ply511 and the glycylglycine endopeptidase lysostaphin were examined. Buffering of the growth media to a neutral pH allowed detection of Ply511 and lysostaphin peptidoglycan hydrolytic activity from all lactic acid bacteria. It was found that purified Ply511 has a pH activity range similar to that of lysostaphin with both enzymes functioning optimally under alkaline conditions. Supernatants from lactobacilli expressing lysostaphin reduced viability of methicillin resistant Staphylococcus aureus (MRSA) by approximately 8 log(10) CFU/ml compared to controls. However, supernatants containing Ply511 were unable to control L. monocytogenes growth. In coculture experiments, both Lb. plantarum and Lb. fermentum synthesizing lysostaphin were able to effectively reduce MRSA cell numbers by >7.4 and 1.7 log(10)CFU/ml, respectively, while lactic acid bacteria secreting Ply511 were unable to significantly inhibit the growth of L. monocytogenes. Our results demonstrate that lysostaphin and Ply511 can be expressed in an active form from different lactic acid bacteria and lysostaphin showed superior killing activity. Lactobacilli producing lysostaphin may have potential for in situ biopreservation in foodstuffs or for prevention of S. aureus infections.
Turner
Mark S
MS
Infectious Diseases Program, School of Life Sciences, Faculty of Science, Queensland University of Technology, G.P.O. Box 2434, Brisbane, Queensland 4001, Australia. ms.turner@qut.edu.au
Waldherr
Florian
F
Loessner
Martin J
MJ
Giffard
Philip M
PM
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D000898:Antibiosis; D001435:Bacteriophages / Q000201:enzymology*; D003001:Cloning, Molecular; D003470:Culture Media; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005516:Food Microbiology; D007778:Lactobacillus / Q000235:genetics; D017045:Lactococcus / Q000235:genetics; D008089:Listeria monocytogenes / Q000502:physiology* / Q000821:virology; D008248:Lysostaphin / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000235:genetics; D050296:Microbial Viability; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000502:physiology*; D014169:Transformation, Bacterial
D000900:Anti-Bacterial Agents; D003470:Culture Media; D011993:Recombinant Fusion Proteins; D010450:Endopeptidases; D008666:Metalloendopeptidases; C400756:soluble secreted endopeptidase; D008248:Lysostaphin; C001143:endolysin