Ethanol-induced pseudohyphal transition in the cells of Candida tropicalis: participation of phosphoinositide signal transduction.
Ethanol-induced pseudohyphal development in the cells of Candida tropicalis Pk233 was accompanied by the transient accumulation of inositol 1,4,5-trisphosphate (IP3) that occurred at an early growth stage. The concomitant addition of myo-inositol prevented the activation of IP3 accumulation and cancelled pseudohyphal development in the presence of ethanol. The addition of a specific phospholipase C inhibitor, U73 122, inhibited ethanol-induced pseudohyphal transition at the concentrations of subinhibitory levels of cell growth. Pseudohyphal development was also induced by the Ca2+ ionophore, A23 187 in the absence of ethanol. The effect of A23 187 on the development of pseudohyphae was little influenced by myo-inositol, but stimulated by concomitant addition of 12-O-tetradecanoylphorbol 13-acetate. These results suggest that ethanol activated phospholipase C in competition with myo-inositol, and the resulting IP3-Ca2+ and protein kinase C pathways of PI signal transduction may work in pseudohyphal transition.
Suzuki
Takahito
T
Department of Biological Sciences, Faculty of Science, Nara Women's University, Nara, Japan. takahito@cc.nara-wu.ac.jp
Omi
Kazuo
K
Ito
Takuya
T
Takata
Toshiya
T
Kikutsugi
Hideo
H
Fujimura
Takaji
T
Kono
Keita
K
Kamihara
Teijiro
T
D041022:Candida tropicalis / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D003470:Culture Media; D004789:Enzyme Activation / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
D003470:Culture Media; D005656:Fungal Proteins; D000431:Ethanol; D015544:Inositol 1,4,5-Trisphosphate; D010740:Phospholipases