Expression of B7-H1 on gastric epithelial cells: its potential role in regulating T cells during Helicobacter pylori infection.
Helicobacter pylori infection is associated with gastritis, ulcers, and gastric cancer. The infection becomes chronic as the host response is unable to clear it. Gastric epithelial cells (GEC) play an important role during the host response, and their expression of class II MHC and costimulatory molecules such as CD80 and CD86 suggests their role in local Ag presentation. Although T cells are recruited to the infected gastric mucosa, they have been reported to be hyporesponsive. In this study, we detected the expression of B7-H1 (programmed death-1 ligand 1), a member of B7 family of proteins associated with T cell inhibition on GEC. Quantitative real-time RT-PCR revealed that B7-H1 expression increased significantly on GEC after H. pylori infection. Western blot analysis showed that B7-H1 expression was induced by various H. pylori strains and was independent of H. pylori virulence factors such as Cag, VacA, and Urease. The functional role of B7-H1 in the cross talk between GEC and T cells was assessed by coculturing GEC or H. pylori-infected GEC with CD4+ T cells isolated from peripheral blood. Using blocking Abs to B7-H1 revealed that B7-H1 was involved in the suppression of T cell proliferation and IL-2 synthesis, and thus suggested a role for B7-H1 on the epithelium as a contributor in the chronicity of H. pylori infection.
Department of Pediatrics, University of Texas Medical Branch, Galveston, TX 77555, USA.
Soumita
S
Das
Giovanni
G
Suarez
Ellen J
EJ
Beswick
Johanna C
JC
Sierra
David Y
DY
Graham
Victor E
VE
Reyes
D015703:Antigens, CD; D018122:B7-1 Antigen / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D060890:B7-H1 Antigen; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D005753:Gastric Mucosa / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D006131:Growth Inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D007700:Kinetics; D008213:Lymphocyte Activation / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D010455:Peptides / Q000235:genetics* / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*
D015703:Antigens, CD; D018122:B7-1 Antigen; D060890:B7-H1 Antigen; C423236:CD274 protein, human; D016207:Cytokines; D006131:Growth Inhibitors; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D010455:Peptides