Antibody-based surface plasmon resonance detection of intact viral pathogen.
Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two-channel (reference & test) micro-fluidic SPR system is used for reliable measurement. Bio-specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors.
Department of Biomedical Engineering, Cornell University, Ithaca, New York 14853, New York, USA.
Hyoungwon
H
Baac
József P
JP
Hajós
Jennifer
J
Lee
Donghyun
D
Kim
Sung June
SJ
Kim
Michael L
ML
Shuler
D000914:Antibodies, Viral; D015374:Biosensing Techniques; D001709:Biotechnology / Q000379:methods; D020349:Surface Plasmon Resonance / Q000379:methods; D014780:Viruses / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity*
D000914:Antibodies, Viral