Transcriptome analysis reveals global expression changes in an industrial L-lysine producer of Corynebacterium glutamicum.
Toward the elucidation of advanced mechanisms of L-lysine production by Corynebacterium glutamicum, a highly developed industrial strain B-6 was analyzed from the viewpoint of gene expression. Northern blot analysis showed that the lysC gene encoding aspartokinase, the key enzyme of L-lysine biosynthesis, was up-regulated by several folds in strain B-6, while no repression mechanism exists in L-lysine biosynthesis of this bacterium. To analyze the underlying mechanisms of the up-regulation, we compared the transcriptome between strain B-6 and its parental wild-type, finding that not only lysC but also many other amino acid-biosynthetic genes were up-regulated in the producer. These results suggest that a certain global regulatory mechanism is involved in the industrial levels of L-lysine production.
Hayashi
Mikiro
M
Biofrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd., Tokyo, Japan.
Ohnishi
Junko
J
Mitsuhashi
Satoshi
S
Yonetani
Yoshiyuki
Y
Hashimoto
Shin-Ichi
S
Ikeda
Masato
M
D002244:Carbon / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D007221:Industry; D008239:Lysine / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
D012333:RNA, Messenger; D002244:Carbon; D008239:Lysine