Pattern of immunoglobulin and T-cell receptor (Ig/TCR) gene rearrangements in Polish pediatric acute lymphoblastic leukemia patients--implications for RQ-PCR-based assessment of minimal residual disease.
We studied 23 Polish children with precursor-B-ALL, using PCR-heteroduplex analysis and DNA sequencing, to determine the availability of Ig/TCR gene rearrangements as patient-specific MRD-RQ-PCR targets. We found IGH, IGK-Kde, incomplete TCRD, Vdelta2-Jalpha, TCRG and TCRB rearrangements in 83%, 39%, 61%, 35%, 61% and 13% of patients, respectively. Comparison of Ig/TCR gene rearrangements pattern (frequency and characteristics of rearrangements) in Polish patients with those reported for patients of other European nationalities did not show major differences. These results are the first promising step for further development of MRD study in Polish patients according to current diagnostic standards.
Dawidowska
Małgorzata
M
Institute of Human Genetics, Polish Academy of Sciences, Department of Molecular and Clinical Genetics, Strzeszyńska 32, 60 479 Poznań, Poland. ma.dawidowska@wp.pl
Derwich
Katarzyna
K
Szczepański
Tomasz
T
Jółkowska
Justyna
J
van der Velden
Vincent H J
VH
Wachowiak
Jacek
J
Witt
Michał
M
D000293:Adolescent; D002051:Burkitt Lymphoma / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics*; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D018365:Neoplasm, Residual; D011044:Poland; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*