Routine markerless gene replacement in Bacillus anthracis.
An improved genetic tool suitable for routine markerless allelic exchange in Bacillus anthracis has been constructed. Its utility was demonstrated by the introduction of insertions, deletions, and missense mutations on the chromosome and plasmid pXO1 of the Sterne strain of B. anthracis.
Janes
Brian K
BK
Division of Bacterial Products, CBER/FDA, 8800 Rockville Pike, Bethesda, MD 20892, USA.
Stibitz
Scott
S
D001408:Bacillus anthracis / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005819:Genetic Markers; D016680:Genome, Bacterial; D010957:Plasmids / Q000235:genetics*
D005819:Genetic Markers