Influence of perfusion on metabolism and matrix production by bovine articular chondrocytes in hydrogel scaffolds.
Articular cartilage has a limited capacity for self-repair after damage. Engineered cartilage is a promising treatment to replace or repair damaged tissue. The growth of engineered cartilage is sensitive to the extracellular culture environment. Chondrocytes were seeded into alginate beads and agarose scaffolds at 4 millions/mL, and the response to static and perfusion culture was examined over period of up to 12 days. For both types of scaffolds, the chondrocytes kept their differentiated morphology over 12 days in all culture conditions. In alginate beads, more glycosaminoglycans (GAGs) were produced in perfusion culture than in static conditions. GAG distribution in alginate constructs was more uniform in perfusion culture than in static culture. However, in agarose constructs there was no significant difference in GAG production between static culture and perfusion culture. Under perfusion culture, the retention rate of GAG in alginate was higher than in agarsoe. It is suggested that the positive effect of perfusion culture only can be achieved by an appropriate choice of other factors such as scaffold materials.
Department of Engineering Science, University of Oxford, Parks Rd., Oxford, OX1 3PJ, United Kingdom.
Xia
X
Xu
Jill P G
JP
Urban
Uday
U
Tirlapur
Min-Hsein
MH
Wu
Zheng
Z
Cui
Zhanfeng
Z
Cui
D000464:Alginates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002358:Cartilage, Articular / Q000166:cytology; D002417:Cattle; D002452:Cell Count; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D004247:DNA / Q000032:analysis; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry; D006025:Glycosaminoglycans / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D010477:Perfusion; D012685:Sepharose / Q000737:chemistry; D046509:Tissue Culture Techniques / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*
D000464:Alginates; D001672:Biocompatible Materials; D006025:Glycosaminoglycans; D006603:Hexuronic Acids; D020100:Hydrogels; D019344:Lactic Acid; D020723:Glucuronic Acid; D003094:Collagen; D004247:DNA; D012685:Sepharose