No age-related change in circulating endothelial progenitor cells in healthy subjects.
Endothelial progenitor cells were isolated from peripheral blood obtained from 32 healthy volunteers without cardiovascular risk factors who ranged in age from 20 to 61 years (mean [+/- SD] age, 34.1 +/- 9.6 years). The fractions of CD34(+) endothelial progenitor cells expressing kinase insert domain receptor-1, CD62E, or CD31 were analyzed with flow cytometry. Correlation analysis demonstrated that there was no significant correlation between subject age and the fraction of circulating CD34(+) mononuclear cells expressing kinase insert domain receptor-1 (P = 0.324; r = -0.180). Similarly, there was no significant correlation between subject age and the fraction of circulating CD34(+) mononuclear cells expressing CD62E (P = 0.496; r = -0.125) or the fraction of circulating CD34(+) mononuclear cells expressing CD31 (P = 0.245; r = -0.212). In conclusion, the experimental results showed that there was no age-related change in the basal level of circulating endothelial progenitor cells in healthy subjects without cardiovascular risk factors.
Chen
Mien-Cheng
MC
Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital Kaohsiung, Kaohsiung, Taiwan, ROC.
Yip
Hon-Kan
HK
Chen
Chien-Jen
CJ
Yang
Cheng-Hsu
CH
Wu
Chiung-Jen
CJ
Cheng
Cheng-I
CI
Chen
Yen-Hsun
YH
Chai
Han-Tan
HT
Lee
Ching-Ping
CP
Chang
Hsueh-Wen
HW
D000328:Adult; D000367:Age Factors; D018952:Antigens, CD34 / Q000097:blood; D019040:E-Selectin / Q000097:blood; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood; D012307:Risk Factors; D013234:Stem Cells / Q000166:cytology*
D018952:Antigens, CD34; D019040:E-Selectin; D019408:Platelet Endothelial Cell Adhesion Molecule-1