Using three-dimensional acinar structures for molecular and cell biological assays.
Epithelial cells grown in three-dimensional matrix allows one to investigate biological processes in a setting that is closer to in vivo conditions than those obtained by growing cells on plastic culture dishes. Here we outline procedures that allow one to investigate molecular mechanisms that regulate formation and disruption of three-dimensional epithelial structures.
Xiang
Bin
B
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
Muthuswamy
Senthil K
SK
D018929:Cell Culture Techniques / Q000379:methods; D003094:Collagen / Q000648:ultrastructure; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism; D004338:Drug Combinations; D004847:Epithelial Cells / Q000648:ultrastructure*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007797:Laminin / Q000648:ultrastructure; D011509:Proteoglycans / Q000648:ultrastructure
D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D051357:Cyclin-Dependent Kinase 2; D051358:Cyclin-Dependent Kinase 4