Transfer of phloem-mobile substances from the host plants to the holoparasite Cuscuta sp.
During the development of the haustorium, searching hyphae of the parasite and the host parenchyma cells are connected by plasmodesmata. Using transgenic tobacco plants expressing a GFP-labelled movement protein of the tobacco mosaic virus, it was demonstrated that the interspecific plasmodesmata are open. The transfer of substances in the phloem from host to the parasite is not selective. After simultaneous application of (3)H-sucrose and (14)C-labelled phloem-mobile amino acids, phytohormones, and xenobiotica to the host, corresponding percentages of the translocated compounds are found in the parasite. An open continuity between the host phloem and the Cuscuta phloem via the haustorium was demonstrated in CLSM pictures after application of the phloem-mobile fluorescent probes, carboxyfluorescein (CF) and hydroxypyrene trisulphonic acid (HPTS), to the host. Using a Cuscuta bridge (14)C-sucrose and the virus PVY(N) were transferred from one host plant to the another. The results of translocation experiments with labelled compounds, phloem-mobile dyes and the virus should be considered as unequivocal evidence for a symplastic transfer of phloem solutes between Cuscuta species and their compatible hosts.
Leibniz Institute of Plant Biochemistry, IPB, Weinberg 3, D-06120 Halle/Saale, Germany. mbirschw@ipb-halle.de
Mandy
M
Birschwilks
Sophie
S
Haupt
Daniel
D
Hofius
Stefanie
S
Neumann
D000596:Amino Acids / Q000378:metabolism; D001692:Biological Transport; D002250:Carbon Radioisotopes; D034182:Cuscuta / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D005456:Fluorescent Dyes / Q000378:metabolism; D006790:Host-Parasite Interactions / Q000502:physiology*; D008954:Models, Biological; D031316:Pelargonium / Q000166:cytology / Q000378:metabolism* / Q000469:parasitology*; D010937:Plant Growth Regulators / Q000378:metabolism; D031425:Plasmodesmata / Q000502:physiology; D013395:Sucrose / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000469:parasitology; D031307:Vicia faba / Q000033:anatomy & histology / Q000469:parasitology; D015262:Xenobiotics / Q000378:metabolism
D000596:Amino Acids; D002250:Carbon Radioisotopes; D005456:Fluorescent Dyes; D010937:Plant Growth Regulators; D015262:Xenobiotics; D013395:Sucrose