Isolation and characterization of mesophilic cellulose-degrading bacteria from flower stalks-vegetable waste co-composting system.
Fifteen mesophilic bacteria with high C(x) cellulase activities were isolated and purified from a mixed-culture enriched from a flower stalks-vegetable waste co-composting system. A CMCase test showed that the enzyme activity of these isolates ranged from 7.9 to 28.0 U ml(-1). Although filter paper degrading capability was low in single culture, significant synergetic cellulose degradation were detected in four groups of mixed cultures, their degradation rates were 23.5%, 26.3%, 19.4% and 24.5%, respectively. Study of morphological and physiological characters of five predominant isolates which possess high CMCase and had positive effect on synergetic cellulose degradation in mixed culture system showed that two of them were closely related to Bacillus pasteurii and Bacillus cereus, whereas the rest belong to the genus Halobacillus, Aeromicrobium and Brevibacterium, respectively.
Lu
Wen-Jing
WJ
Department of Environmental Science and Engineering, Tsinghua University, Beijing, PR China. luwenjing@tsinghua.edu.cn
Wang
Hong-Tao
HT
Yang
Shi-Jian
SJ
Wang
Zhi-Chao
ZC
Nie
Yong-Feng
YF
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D002482:Cellulose / Q000378:metabolism*; D035264:Flowers / Q000378:metabolism*; D012037:Refuse Disposal; D014675:Vegetables / Q000382:microbiology*
D002482:Cellulose