Enzyme induction in cryopreserved human hepatocyte cultures.
Freshly isolated human hepatocytes are considered as the gold standard for in vitro testing of drug candidates. Meanwhile also cryopreserved human hepatocyte suspensions are available. However, a drawback of these cells is the incalculability of attachment to the culture dish. Therefore, we established a technique freezing hepatocytes cultured on a collagen gel. After thawing damaged cells were removed to a certain extent by gentle washing with culture medium prior to adding an upper gel layer. The morphology of the resulting hepatocyte cultures could not be distinguished from that of non-frozen cells. However, basal activities of cytochrome P450 isoforms decreased in cryopreserved compared to non-frozen hepatocytes, as evidenced by analysis of testosterone hydroxylation (OHT) in positions 6beta, 16alpha, 2beta and 6alpha. Nevertheless, enzyme induction factors caused by 24 h incubation with 50 microM rifampicin were similar in cryopreserved and non-frozen hepatocytes. In cryopreserved hepatocytes rifampicin caused an increase in mean values of 6beta-OHT formation from 57.2 to 157.7 pmol/well/min (2.8-fold), compared to an increase from 115.8 to 269.1 pmol/well/min (2.3-fold) in non-frozen cells. Similarly, 16alpha- and 2beta-OHT showed induction factors of 2.4- and 2.3-fold in cryopreserved compared to 1.6- and 2.4-fold in non-frozen hepatocytes, respectively. In conclusion, human hepatocytes cryopreserved on collagen gels show a clear induction of CYP3A4 by rifampicin, although the basal activities are reduced compared to non-frozen cells.
Cytonet GmbH & Co., KG Hannover Branch, Feodor Lynen Strasse 21, D-30625 Hannover, Germany. sabine.kafert-kasting@cytonet.de
Sabine
S
Kafert-Kasting
Krassimira
K
Alexandrova
Marc
M
Barthold
Britta
B
Laube
Gerhard
G
Friedrich
Lubomir
L
Arseniev
Jan G
JG
Hengstler
D000368:Aged; D018929:Cell Culture Techniques; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen; D015925:Cryopreservation; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction; D005260:Female; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D006900:Hydroxylation; D008297:Male; D008875:Middle Aged; D012293:Rifampin / Q000494:pharmacology*; D013739:Testosterone / Q000378:metabolism
D013739:Testosterone; D003094:Collagen; D003577:Cytochrome P-450 Enzyme System; C104464:CYP3A protein, human; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human; D012293:Rifampin