Alterations of the CCND1 and HER-2/neu (ERBB2) proteins in esophageal and gastric cancers.
We evaluated the relationship of amplification and polysomy of both the CCND1 and the ERBB2 (alias HER-2/NEU) genes to the overexpression of their proteins in esophageal and gastric cancers and also their association with clinicopathological features. CCND1 gene amplification (45%) was more prevalent than polysomy (25%) in esophageal carcinoma, but the pattern observed was similar in gastric adenocarcinoma (10% amplification, 15% polysomy). For ERBB2, polysomy was a more frequent mechanism than amplification in both esophageal (32.5 vs. 7.5%) and gastric (15 vs. 5%) cancers. Overexpression of cyclin D1 protein was identified in 37.5% of the specimens of esophageal tumors and 35% of gastric tumors, and overexpression of Her-2/neu protein in 12.5 and 7.5%, respectively. The kappa-statistics revealed a fair agreement in both types of tumors only in overexpression and amplification of the CCND1 gene; the ERBB2 gene showed a fair agreement in amplification and polysomy and the level of protein expression in gastric adenocarcinoma. Thus, polysomy 17 could contribute to a high Her-2/neu protein level, at least in gastric cancer. Our data indicated an association with alcohol consumption and the CCND1 gene or protein levels, in both esophageal and gastric cancers.
Department of Biology, São Paulo State University-UNESP, Rua Cristóvão Colombo, 2265, 15054-000, São José do Rio Preto, SP, Brazil.
Lucimari
L
Bizari
Aldenis Albanese
AA
Borim
Kátia Ramos Moreira
KR
Leite
Fernanda de Toledo
Fde T
Gonçalves
Patrícia Maluf
PM
Cury
Eloiza Helena
EH
Tajara
Ana Elizabete
AE
Silva
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D019938:Cyclin D1 / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000382:microbiology / Q000401:mortality / Q000473:pathology; D005260:Female; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D018734:Genes, erbB-2 / Q000235:genetics*; D016481:Helicobacter Infections / Q000453:epidemiology; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000382:microbiology / Q000401:mortality / Q000473:pathology; D016019:Survival Analysis
D019938:Cyclin D1; D018719:Erb-b2 Receptor Tyrosine Kinases