Agrobacterium tumefaciens-mediated transformation of Mucor circinelloides.
The Agrobacterium tumefaciens-mediated transformation of the zygomycetous fungus Mucor circinelloides is described. A method was also developed for the hygromycin B-based selection of Mucor transformants. Transformation with the hygromycin B phosphotransferase gene of Escherichia coli controlled by the heterologous Aspergillus nidulans trpC promoter resulted in hygromycin B-resistant clones. The presence of the hygromycin resistance gene in the genome of the transformants was verified by polymerase chain reaction and Southern hybridization: the latter analyses revealed integrations in the host genome at different sites in different transformants. The stability of transformants remained questionable during the latter analyses.
HAS-USZ Microbiological Research Group, Department of Microbiology, Faculty of Science, University of Szeged, 6701 Szeged, Hungary.
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Nyilasi
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Acs
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Papp
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Nagy
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Vágvölgyi
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000935:Antifungal Agents / Q000494:pharmacology; D001233:Aspergillus nidulans / Q000235:genetics; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D025141:Drug Resistance, Fungal / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D016681:Genome, Fungal / Q000235:genetics; D006921:Hygromycin B / Q000494:pharmacology; D009089:Mucor / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D012641:Selection, Genetic; D014170:Transformation, Genetic
D000935:Antifungal Agents; D004271:DNA, Fungal; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D006921:Hygromycin B; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase