Rapid high-throughput detection of peroxide with an acridinium-9-carboxamide: a homogeneous chemiluminescent assay for plasma choline.
Hydrogen peroxide generated from the enzymatic oxidation of choline was detected using a chemiluminescent acridinium-9-carboxamide. The dose-response for choline (0-50 microM) was established in buffer and was applicable to the quantification of choline in human plasma. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (1 microL) and analysis time (<5 s per well).
Adamczyk
Maciej
M
Abbott Laboratories, Diagnostics Division, 100 Abbott Park Road, AP20, Abbott Park, IL 60064-6016, USA. maciej.adamczyk@abbott.com
Brashear
R Jeffrey
RJ
Mattingly
Phillip G
PG
D000166:Acridines / Q000737:chemistry*; D001622:Betaine / Q000138:chemical synthesis; D002794:Choline / Q000097:blood* / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D008163:Luminescent Measurements / Q000379:methods*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D013997:Time Factors
D000166:Acridines; C402305:acridinium-9-carboxamide; D001622:Betaine; D006861:Hydrogen Peroxide; D002794:Choline