Anaplasma phagocytophilum infection reduces expression of phagocytosis-related receptors on neutrophils.
Anaplasma phagocytophilum is a tick-transmitted obligate intracellular bacterium of neutrophils that causes human granulocytic anaplasmosis. Previous data confirm that in vitro infection by A. phagocytophilum modifies neutrophil functions, including a 50% or greater reduction in phagocytosis and shedding of neutrophil cell surface adhesion molecule receptors. If these receptors are downregulated or shed from the surface of neutrophils with A. phagocytophilum infection, it may prevent neutrophils from effective phagocytosis. We hypothesized that diminished phagocytosis in neutrophils is partly associated with the loss of surface phagocytic receptors. To address this, we assayed the expression of these receptors after 24 h of A. phagocytophilum infection in human neutrophils.
Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Justin W A
JW
Garyu
J Stephen
JS
Dumler
D041081:Anaplasma phagocytophilum; D018922:HL-60 Cells; D006801:Humans; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D010587:Phagocytosis / Q000276:immunology*; D011951:Receptors, Complement / Q000096:biosynthesis / Q000235:genetics; D011961:Receptors, Fc / Q000096:biosynthesis / Q000235:genetics; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics*
D011951:Receptors, Complement; D011961:Receptors, Fc; D011971:Receptors, Immunologic; C028495:phagocytosis receptor