Glucocorticoids suppress proteoglycan production by human tenocytes.
BACKGROUND
The role of glucocortiocid injection therapy in spontaneous tendon rupture is controversial. We hypothesized that glucocorticoids suppress proteoglycan production in tendon and studied the in vitro effects of dexamethasone and triamcinolone on proteoglycan production by cultured human tenocytes.


METHODS
We obtained primary cultures of human tenocytes from explants of healthy human patellar tendon. The human tenocytes were treated with 1 microM dexamethasone or 1 microM triamcinolone. The amount of proteoglycan production was measured by 35S-sulfate incorporation assay and compared with control cultures. The reversibility of the effect of dexamethasone by co-incubation with 10 ng platelet-derived growth factor (PDGFBB) was also tested.


RESULTS
Treatment with 1 microM triamcinolone reduced the amount of 35S-sulfate incorporation to 80% of control cultures (p = 0.007), whereas 1 microM dexamethasone reduced it to 72% (p = 0.01). Co-incubation of 10 ng/mL PDGFBB with 1 microM dexamethasone returned the 35S-sulfate incorporation to a level that was significantly higher than for dexamethasone treatment alone (108%; p = 0.01).


CONCLUSIONS
Glucocorticoids suppressed proteoglycan production in cultured human tenocytes. The suppression by dexamethasone was reversed by simultaneous addition of PDGFBB. Suppressed proteoglycan production may affect the viscoelastic properties of tendon and increase the risk of spontaneous rupture.
Wong
Margaret Wan Nar
MW
Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, Hong Kong. mwnwong@cuhk.edu.hk
Tang
Yvonne Yin Nei
YY
Lee
Simon Kwong Man
SK
Fu
Bruma Sai Chuen
BS
D002478:Cells, Cultured; D003907:Dexamethasone / Q000009:adverse effects / Q000494:pharmacology; D005938:Glucocorticoids / Q000009:adverse effects / Q000494:pharmacology*; D006801:Humans; D010329:Patella; D011509:Proteoglycans / Q000096:biosynthesis*; D013708:Tendon Injuries / Q000139:chemically induced; D013710:Tendons / Q000166:cytology / Q000187:drug effects*
D005938:Glucocorticoids; D011509:Proteoglycans; D003907:Dexamethasone