Purification and characterization of Mycobacterium tuberculosis indole-3-glycerol phosphate synthase.
Indole-3-glycerol phosphate synthase (IGPS) plays an important role in the survival of Mycobacterium tuberculosis. The trpC gene, encoding IGPS, is essential for the growth of M. tuberculosis. It was expressed at the transcriptional level in cultured M. tuberculosis. The recombinant IGPS with an added His-tag was purified. The His-tag was found to have a small effect on the biochemical properties of IGPS. IGPS is a monofunctional enzyme in M. tuberculosis. Recombinant IGPS has considerable beta-pleated sheet and is relatively compact. The enzyme activity is significantly inhibited by denaturants and antibiotics, suggesting that IGPS may be a novel potential drug target of M. tuberculosis.
Yang
Yanping
Y
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, PR China.
Zhang
Min
M
Zhang
Hongmei
H
Lei
Jianqiang
J
Jin
Ruiliang
R
Xu
Shengfeng
S
Bao
Jialing
J
Zhang
Lu
L
Wang
Honghai
H
D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000737:chemistry; D007209:Indole-3-Glycerol-Phosphate Synthase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D017433:Protein Structure, Secondary
D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D007209:Indole-3-Glycerol-Phosphate Synthase