A role for Efg1p in Candida albicans interactions with extracellular matrices.
Candida albicans interactions with extracellular matrices extracellular matrices represent hallmarks of invasive candidiasis. We have assessed the role of Efg1p, a key transcriptional regulator, in the interactions between C. albicans and extracellular matrices. Wild-type C. albicans cells were able to bind and subsequently penetrate the extracellular matrices layer, whereas the Deltaefg1/Deltaefg1 mutant strain showed a drastically reduced ability to interact with extracellular matrices. Results indicate that, besides its control of morphogenesis, Efg1p also regulates C. albicans interactions with extracellular matrices. Proteomic analyses indicated that expression of 30 surface-associated proteins is affected in the Deltaefg1/Deltaefg1 mutant, some of which may be involved in adherence to extracellular matrices.
Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA.
Stephen P
SP
Saville
Derek P
DP
Thomas
José L
JL
López Ribot
D002176:Candida albicans / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005109:Extracellular Matrix / Q000235:genetics / Q000382:microbiology* / Q000502:physiology; D005340:Fibrinogen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005656:Fungal Proteins / Q000502:physiology*; D007797:Laminin / Q000378:metabolism; D008565:Membrane Proteins / Q000145:classification / Q000502:physiology*; D009154:Mutation / Q000502:physiology; D040901:Proteomics / Q000379:methods; D019063:Tenascin / Q000378:metabolism; D014157:Transcription Factors / Q000502:physiology*
D004268:DNA-Binding Proteins; C121438:EFG1 protein, Candida albicans; D005353:Fibronectins; D005656:Fungal Proteins; D007797:Laminin; D008565:Membrane Proteins; D019063:Tenascin; D014157:Transcription Factors; D005340:Fibrinogen