Inactivation of Saccharomyces cerevisiae and polyphenoloxidase in mango nectar treated with UV light.
Fresh mango nectar was processed by UV light at five flow rates (0.073 to 0.451 liter/min) and five UV light doses (75 to 450 kJ/m2) to evaluate total microbial load, Saccharomyces cerevisiae survival, and polyphenoloxidase activity. UV systems containing an inner mercury lamp (254 nm) each with intensity of 25 mW/cm2 were used as germicidal sources. In addition, mango nectar was treated for 15 min at 0.073 and 0.451 liter/min, stored at 3 degrees C, and evaluated periodically for total microbial count, yeast count, color, and polyphenoloxidase activity. The first-order kinetics modeling found that DUV-values in mango nectar ranged from 27.9 to 10.9 min (R2 > 0.950) and 26.0 to 11.8 min (R2 > 0.962) for total microbial count and yeast count, respectively. The maximum log reduction (CFU per milliliter) was 2.71 and 2.94 for total microbial count and yeast count, respectively, after 30 min of UV treatment at 0.451 liter/min. DUV-values ranging from 156 to 204 min were observed for polyphenoloxidase activity. The remaining polyphenoloxidase activity after 30 min of UV treatment at 0.451 liter/min was 19 +/- 4%. Initial microbial load and yeast in stored mango nectar were reduced in the range 2.86 to 3.41 and 1.82 to 1.97 log (CFU/ml) cycles, respectively. No substantial microbial growth was observed prior to 20 days of storage. Averages of 1,055 +/- 32, 803 +/- 32, and 710 +/- 37 enzyme activity units were observed in mango nectar UV processed at 0, 0.073, and 0.451 liter/min, respectively, during the entire storage period. However, mango nectar treated at 0.073 and 0.451 liter/min maintained a yellow and yellow-orange color, respectively, after 26 days of storage.
Biological Systems Engineering Department, Washington State University, Pullman 99164-6120, USA.
José A
JA
Guerrero-Beltrán
Gustavo V
GV
Barbosa-Cánovas
D004156:Catechol Oxidase / Q000378:metabolism / Q000528:radiation effects*; D015169:Colony Count, Microbial; D004307:Dose-Response Relationship, Radiation; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D031022:Mangifera / Q000201:enzymology / Q000382:microbiology*; D010858:Pigmentation; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000528:radiation effects*; D013997:Time Factors; D014466:Ultraviolet Rays
D004156:Catechol Oxidase