Identification, cloning, and expression of potential diagnostic markers for Q fever.
The clinical diagnosis of Q fever is difficult. Whole cell antigens are currently used in several serological methods, but antigens are limited due to the hazardous nature of Coxiella burnetii cultivation. In this report, we described the method of detecting immunodominant antigens of C. burnetii by using proteomic techniques with patient sera, and cloning and expressing the selected antigens using a novel vector known for its ease of expression, purification, and downstream application.
Rickettsial Disease Department, Naval Medical Research Center, Silver Spring, MD 20910, USA. chaoc@nmrc.navy.mil
C C
CC
Chao
H W
HW
Chen
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Li
W B
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Xu
B
B
Hanson
W M
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Ching
D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D003001:Cloning, Molecular; D016997:Coxiella burnetii / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D011778:Q Fever / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers