Basic methods for fission yeast.
The fission yeast Schizosaccharomyces pombe is a popular model system, and has been particularly influential in studies of the cell cycle and chromosome dynamics. Despite its differences from Saccharomyces cerevisiae, the tools and methods for fission yeast are conceptually similar to those used in budding yeast. Here, we present basic methods sufficient for a beginner in this system to carry out most required manipulations for genetic analysis or molecular biology.
Forsburg
Susan L
SL
Program in Molecular and Computational Biology, University of Southern California, 1050 Child's Way MCB201, Los Angeles, CA 90089-2910, USA. forsburg@usc.edu
Rhind
Nicholas
N
D003433:Crosses, Genetic; D008967:Molecular Biology / Q000379:methods; D009172:Mycology / Q000379:methods; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
D029702:Schizosaccharomyces pombe Proteins