Fourier-transform coherent anti-Stokes Raman scattering microscopy.
We report a novel Fourier-transform-based implementation of coherent anti-Stokes Raman scattering (CARS) microscopy. The method employs a single femtosecond laser source and a Michelson interferometer to create two pulse replicas that are fed into a scanning multiphoton microscope. By varying the time delay between the pulses, we time-resolve the CARS signal, permitting easy removal of the nonresonant background while providing high resolution, spectrally resolved images of CARS modes over the laser bandwidth (approximately 1500 cm(-1)). We demonstrate the method by imaging polystyrene beads in solvent.
Laboratoire d'Optique et Biosciences, Centre National de la Recherche Scientifique Unité Mixte de Recherche 7645, Institut National de la Santé et de la Recherche Médicale U696, Ecole Polytechnique, 91128 Palaiseau, France. jogilvie@umich.edu
Jennifer P
JP
Ogilvie
Emmanuel
E
Beaurepaire
Antigoni
A
Alexandrou
Manuel
M
Joffre
D000465:Algorithms; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005583:Fourier Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D016247:Information Storage and Retrieval / Q000379:methods*; D008027:Light; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D008857:Microscopy, Interference / Q000295:instrumentation* / Q000379:methods; D019047:Phantoms, Imaging; D012542:Scattering, Radiation; D012815:Signal Processing, Computer-Assisted; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods