Purification and properties of rab6 interacting proteins.
A crucial step in the characterization of novel partners of Rab proteins is the confirmation that they indeed interact together by techniques other than the yeast two-hybrid assay used to discover them. Some methods and clues that would help to discriminate between putative interactors are summarized. Pull-down, co-immunoprecipitation, and gel filtration experiments are described as ways of checking protein-protein interaction in vitro and in vivo.
Solange
S
Monier
Bruno
B
Goud
D048868:Adaptor Proteins, Signal Transducing / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D047468:Immunoprecipitation; D011485:Protein Binding; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; C094135:Rab6 protein; D020691:rab GTP-Binding Proteins