Telomerase peptide vaccination: a phase I/II study in patients with non-small cell lung cancer.
OBJECTIVE
A phase I/II study was conducted to investigate the safety, tolerability and clinical response to vaccination with a combination of telomerase peptides GV1001 (hTERT: 611-626) and HR2822 (hTERT: 540-548) in patients with non-small cell lung cancer.


METHODS
Twenty-six patients with non-small cell lung cancer received intradermal administrations of either 60 nmole (112 microg) or 300 nmole (560 microg) GV1001 in combination with 60 nM (68.4 microg) HR2822 and granulocyte macrophage-colony stimulating factor. The treatment period was 10 weeks. Booster vaccinations with 300 nM GV1001 were offered as follow-up. Monitoring of blood samples, clinical examination and radiological staging were performed regularly. Immune responses were measured as delayed-type hypersensitivity skin reaction and in vitro T cell proliferation. Bone marrow function was monitored in long time survivors.


RESULTS
The treatment was well tolerated with minor side effects. No bone marrow toxicities were observed in long time survivors with immune responses. Immune responses against GV1001 were detected in 11 of 24 evaluable patients during the primary regimen and in additional two patients following booster injections. Two patients responded to HR2822. Cloned GV1001-specific CD4+ T cells displayed a Th1 cytokine profile and recognized autologous antigen presenting cells pulsed with recombinant telomerase protein. A complete tumor response was observed in one patient who developed GV1001-specific cytotoxic T cells that could be cloned from peripheral blood.


CONCLUSIONS
The results demonstrate that GV1001 and HR2822 are immunogenic and safe to use in patients with NSCLC. Induction of GV1001-specific immune responses may result in objective tumor responses. Based on these initial encouraging results, further clinical studies of GV1001 in NSCLC patients are warranted.
Brunsvig
Paal F
PF
Norwegian Radium Hospital, University of Oslo, Oslo, Norway.
Aamdal
Steinar
S
Gjertsen
Marianne K
MK
Kvalheim
Gunnar
G
Markowski-Grimsrud
Carrie J
CJ
Sve
Ingunn
I
Dyrhaug
Marianne
M
Trachsel
Sissel
S
Møller
Mona
M
Eriksen
Jon A
JA
Gaudernack
Gustav
G
D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000187:drug effects; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000009:adverse effects / Q000276:immunology* / Q000627:therapeutic use; D002289:Carcinoma, Non-Small-Cell Lung / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000276:immunology; D005260:Female; D006801:Humans; D016233:Immunotherapy, Active; D008175:Lung Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy*; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000009:adverse effects / Q000276:immunology* / Q000627:therapeutic use; D010455:Peptides / Q000276:immunology; D019098:Telomerase / Q000009:adverse effects / Q000276:immunology* / Q000627:therapeutic use; D014057:Tomography, X-Ray Computed
D019496:Cancer Vaccines; D010446:Peptide Fragments; D010455:Peptides; C514987:GV1001 peptide; C437311:telomerase reverse transcriptase (540-548); D019098:Telomerase