An investigation on the possible role of melatonin in melanogenesis.
The possible role of melatonin in melanogenesis was investigated by performing reactions of melatonin with peroxidase + H2O2 or H2O2 only, in the presence or absence of UV irradiation. Samples of the reaction mixtures were drawn at different times (from 15 to 480 min), the enzyme (when present) was removed by ultrafiltration and the samples so obtained were analyzed by MALDI/MS. The results show that melatonin undergoes oligomerization reaction with peroxidase + H2O2, leading to heptameric species. For high reaction times the MALDI/MS data do not show the formation of larger oligomers, but UV-vis spectroscopy indicates that the oligomerization processes proceed. The failure of MALDI-TOF in the identification of larger oligomers was related to the chemical-physical and morphological behavior of melanins. In the case of UV irradiation, the formation of species originating from the O- and O2 addition to melatonin, which activate new oligomerization channels, has been observed.
Rizzi
Andrea
A
Consiglio Nazionale delle Ricerche, ISTM, Corso Stati Uniti 4, 35100 Padova, Italy.
Comai
Stefano
S
Bertazzo
Antonella
A
Costa
Carlo V L
CV
Allegri
Graziella
G
Traldi
Pietro
P
D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000528:radiation effects; D008543:Melanins / Q000737:chemistry* / Q000528:radiation effects*; D008550:Melatonin / Q000737:chemistry* / Q000528:radiation effects*; D008956:Models, Chemical; D008958:Models, Molecular; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014466:Ultraviolet Rays
D008543:Melanins; D006861:Hydrogen Peroxide; D008550:Melatonin