Effects of parathyroid hormone on newly regenerated bone during distraction osteogenesis in a rabbit tibial lengthening model. A pilot study.
OBJECTIVE
The overall purpose of the study is to determine the effects of parathyroid hormone (PTH) (1-34) on bone formation in regenerated and surrounding bone of distracted callus during limb lengthening in rabbits. Additionally the aim of the pilot study is to titrate the optimal dose of PTH for distraction osteogenesis treatment in rabbits' tibial lengthening model.


METHODS
A total of 18 rabbits underwent right tibia lengthening by callus distraction. Lengthening was started 5 days postoperatively 1 mm/day for a 10-day period and consolidation of 20 days followed. Rabbits were divided into three groups: group I received PTH (1-34) treatment at a dose of 5 microg/kg/day, group II received treatment with PTH (1-34) at a dose of 25 microg/kg/day; group III rabbits were treated with saline. After euthanasia, tibiae of both legs were dissected free, kept frozen and underwent x-ray analysis, dual x-ray absorptiometry-scanning, microcomputed tomography scanning and three-dimensional evaluation and mechanical test followed.


RESULTS
Over all, during distraction osteogenesis in a new regenerated bone, PTH (1-34) treatment with two different doses of 5 microg/kg/day and 25 microg/kg/day increased callus cross-sectional area, callus bone mineral density and bone mineral content, bone volume density; dramatically increased trabecular number with slight increase in trabecular thickness, whereas decreased trabecular separation, bone surface density and decreased degree of anisotropy when compared to control group animals.


CONCLUSIONS
PTH (1-34) treatment improved mineralization, structural indices of regenerated distracted rabbits' tibiae, whereas treatment at a dose of 25 microg/kg/day PTH (1-34) was significantly more effective than 5 microg/kg/day PTH(1-34) dose treatment when compared to control group. Bigger dose has been chosen for the main study.
Aleksyniene
Ramune
R
Department of Orthopedics, Aalborg University Hospital, Denmark. ramunealeks@vision.kmu.lt
Eckardt
Henrik
H
Bundgaard
Kristian
K
Lind
Martin
M
Hvid
Ivan
I
D015502:Absorptiometry, Photon; D000818:Animals; D015519:Bone Density; D001852:Bone Lengthening / Q000295:instrumentation / Q000379:methods*; D001861:Bone Regeneration / Q000187:drug effects*; D016267:External Fixators; D005260:Female; D005500:Follow-Up Studies; D010012:Osteogenesis; D010027:Osteotomy; D010281:Parathyroid Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D010865:Pilot Projects; D011817:Rabbits; D013977:Tibia / Q000601:surgery*; D013997:Time Factors; D014057:Tomography, X-Ray Computed
D010281:Parathyroid Hormone