Contribution of linseed intake to urine and serum enterolignan levels in German females: a randomised controlled intervention trial.
Linseeds are a rich source of lignans, secondary plant substances which are suggested to possess chemopreventive effects inter alia with regard to breast cancer. In a randomised controlled trial 40 German women were informed about "5-a-day" and encouraged to increase their dietary intake of fruit and vegetables. Moreover 19 participants were randomly assigned to an intervention group supplemented with ground linseeds (20 g/d) over a 2-month period. Before and after intervention, urine and blood samples were collected after an overnight fast. Analysis was by intention-to-treat and the outcome parameters of interest were enterolignan concentrations. After linseed supplementation, enterolignan concentrations (mean) measured as their glucuronides by a newly developed high performance liquid chromatography electrospray mass spectrometry (HPLC-ESI-MS) in serum (122 nmol/l) as well as in urine (72 micromol/l) showed a significant increase (P<0.01) compared to pre-intervention values (47 nmol/l and 29 micromol/l). In the control group enterolignan levels were raised slightly but did not reach significance. Serum and urinary enterolignans of the whole collective showed a good pairwise correlation.
Division of Toxicology and Cancer Risk Factors, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany. u.knust@dkfz-heidelberg.de
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Knust
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Spiegelhalder
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Strowitzki
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Owen
D000328:Adult; D002072:Butylene Glycols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D019587:Dietary Supplements; D005260:Female; D005638:Fruit; D005858:Germany; D005960:Glucosides / Q000032:analysis; D006801:Humans; D017705:Lignans / Q000097:blood* / Q000652:urine*; D008043:Linseed Oil / Q000737:chemistry / Q000494:pharmacology*; D008875:Middle Aged; D021241:Spectrometry, Mass, Electrospray Ionization; D014675:Vegetables
D002072:Butylene Glycols; D005960:Glucosides; D017705:Lignans; D008043:Linseed Oil; C090142:secoisolariciresinol diglucoside