Confocal reflection imaging of 3D fibrin polymers.
The reconstruction of extracellular matrix (ECM) by confocal reflection microscopy is a physical approach for monitoring the ECM structure, cell-matrix interactions and changes of ECM structure over time. We here show that confocal reflection imaging is useful in reconstructing the fibrillar architecture of 3D fibrin lattices and fibroblasts embedded therein at high resolution up to 250 nm. Together with confocal fluorescence microscopy of collagen deposited by fibroblasts, this technique allows the monitoring of fibrin remodeling by stromal cells. In conclusion, confocal reflection microscopy is a valuable technique for real-time monitoring of the remodeling of fibrin matrices, such as during thrombus formation, wound healing, and tumor formation.
Hartmann
Anke
A
Department of Dermatology and Rudolf Virchow Center for Experimental Biomedicine, University of Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, Germany.
Boukamp
Petra
P
Friedl
Peter
P
D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D005337:Fibrin / Q000737:chemistry*; D005347:Fibroblasts; D006801:Humans; D021621:Imaging, Three-Dimensional; D018613:Microscopy, Confocal; D011487:Protein Conformation; D017154:Stromal Cells
D005337:Fibrin; D003094:Collagen