[Genetic transformation of peach immature cotyledons with its antisenes ACO gene].
Genetic transformation of peach immature cotyledons with its ACO antisense gene was studied by using particle bombardment method through Agrobacterium tumefaciens. Kanr shoots and Kanr plantlet were obtained. The plantet with ACO antisenes gene through Agrobacterium tumefaciens was obtained by micrografting technique and survived for nearly one month. The results of the PCR, PCR-southern, genomes southern hybridization analysis and GUS color reaction of some Kanr materials showed in some degree that the peach ACO antisens gene was integrated into peach genomes.
Wu
Yan-Jun
YJ
Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hongzhou 310029, China. wyjwjht@163.com
Zhang
Shang-Long
SL
Xie
Ming
M
Chen
Jun-Wei
JW
Jiang
Gui-Hua
GH
Qin
Yong-Hua
YH
Qin
Qiao-Ping
QP
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D018548:Cotyledon / Q000235:genetics*; D016373:DNA, Antisense / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D016133:Polymerase Chain Reaction; D027861:Prunus / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics*
D016373:DNA, Antisense; D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase