Differential expression of neuregulins and their receptors in the olfactory bulb layers of the developing mouse.
Neuregulins (NRGs), and their cognate receptors (ErbBs), play essential roles in numerous aspects of neural development and adult synaptic plasticity. The goal of this study was to investigate the developmental expression profiles of these molecules during the olfactory bulb (OB) maturation. The OB is a highly organized structure with cell types and synaptic connections segregated into discrete anatomical layers. We employed a novel approach by combining single-layer microdissection at different development ages, with isoform-specific semi-quantitative RT-PCR and Western blotting to monitor layer-specific developmental profiles of these molecules and alternate splice variants. Layer and age specific regulation was observed for the ErbB4 splice variants JMa/JMb and NRG-1-beta1/beta2 forms. With the exception of the outermost (nerve) layer, ErbB4-JMb and NRG-1-beta1 are expressed throughout the OB and their expressions decrease in the adult age in most layers. In contrast both ErbB4-JMa and NRG-1-beta2 are highly expressed in the granule cell layer in the early postnatal OB. This early postnatal expression correlates with the dramatic change from radial glia to astrocytes and appearance of the bulk of granule cells occurring at this developmental stage.
Bovetti
Serena
S
Department of Human and Animal Biology, University of Torino, Via Accademia Albertina 13, 10123 Torino, Italy.
De Marchis
Silvia
S
Gambarotta
Giovanna
G
Fasolo
Aldo
A
Perroteau
Isabelle
I
Puche
Adam C
AC
Bovolin
Patrizia
P
D000367:Age Factors; D017398:Alternative Splicing / Q000502:physiology; D000818:Animals; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D020880:Neuregulins / Q000235:genetics / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020880:Neuregulins; D020033:Protein Isoforms; D012333:RNA, Messenger; D066246:ErbB Receptors