[Changes in cyclooxygenase gene expression during spontaneous recovery from stress ulcer in rats].
OBJECTIVE
To observe the changes in gene expression of cyclooxygenase (COX) during spontaneous recovery from stress ulcer in rats exposed to water immersion and restraint stress (WRS).


METHODS
A rat model of stress ulcer was established by means of WRS, in which the changes in COX expression were detected with immunohistochemistry and reverse transcription (RT)-PCR.


RESULTS
Very low levels of COX-2 expression were detected in the gastric mucosa of the control rats, and the expression increased significantly during the healing process of the stress ulcer (P<0.05). COX-1 expression in the gastric mucosa showed no significant difference between the control group and the stress ulcer groups during healing (P>0.05).


CONCLUSIONS
COX-1 and COX-2 expressions in rat gastric mucosa during the recovery from stress ulcer participate in the recovery of the damaged mucosa possibly by mediating prostaglandin secretion.
Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. xzy1996@fimmu.com
Jun
J
Xu
Yu-gang
YG
Song
Xian-fu
XF
Sang
Guang-xin
GX
Bao
Xu
X
Li
Gang
G
Wu
Dong-shen
DS
Chen
D000818:Animals; D051545:Cyclooxygenase 1 / Q000096:biosynthesis / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D005753:Gastric Mucosa / Q000201:enzymology; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012075:Remission, Spontaneous; D013276:Stomach Ulcer / Q000201:enzymology*; D013312:Stress, Physiological / Q000150:complications
D012333:RNA, Messenger; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases