Difference of growth-inhibitory effect of Scutellaria baicalensis-producing flavonoid wogonin among human cancer cells and normal diploid cell.
Methanol extract from cultured Scutellaria baicalensis cells inhibited the proliferation of human monocytic leukemia cell line THP-1 and human osteogenic sarcoma cell line HOS. The inhibitory effects of baicalin, baicalein and wogonin, the three major flavonoids contained in the extract, were studied. It should be noted that wogonin did not show the inhibitory effect on human fetal lung normal diploid cell line TIG-1, as compared to the inhibition observed in cancer cells. Physiological analyses in THP-1 cells showed that wogonin induced cell cycle arrest at G(2)/M phase and apoptosis. This is the first report discovering a cancer-specific apoptosis-inducing activity of wogonin.
Department of Biotechnology, Faculty of Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan.
Mika
M
Himeji
Takashi
T
Ohtsuki
Harumi
H
Fukazawa
Miho
M
Tanaka
Shin-Ichi
S
Yazaki
Sadaharu
S
Ui
Koji
K
Nishio
Hisako
H
Yamamoto
Kachio
K
Tasaka
Akio
A
Mimura
D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004171:Diploidy; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D044950:Flavanones / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology; D016195:G2 Phase / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D031364:Scutellaria baicalensis / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D006131:Growth Inhibitors; D010936:Plant Extracts; C038044:baicalin; C006680:baicalein; C085514:wogonin