Jacalin and peanut agglutinin (PNA) bindings in the taste bud cells of the rat: new reliable markers for type IV cells of the rat taste buds.
Lectin histochemistry of Jacalin (Artocarpus integrifolia) and peanut agglutinin (PNA), specific lectins for galactosyl (beta-1, 3) N-acetylgalactosamine (galactosyl (beta-1, 3) GalNAc), was applied to the gustatory epithelium of the adult rat. In the ordinary lingual epithelium, Jacalin and PNA labeled the cell membrane from the basal to granular cell layer. They also bound membranes of rounded-cells at the basal portion of taste buds, but the number of PNA labeled cells was smaller than that of Jacalin labeled cells. There was no apparent difference in the binding patterns of Jacalin and PNA among the taste buds of the lingual papillae and those of the palatal epithelium. Occasionally, a few spindle-shaped cells were labeled with Jacalin, but not with PNA. Double labeling of Jacalin and alpha-gustducin, a specific marker for type II cells, revealed that Jacalin-labeled spindle-shaped taste cells were immunonegative for alpha-gustducin. Spindle-shaped cells expressing protein gene product 9.5 (PGP 9.5) immunoreactivity lacked Jacalin labeling. During the development of taste buds in circumvallate papillae, the binding pattern of Jacalin became almost identical from postnatal day 5. The present results indicate that rounded cells at the basal portion of the taste buds cells (type IV cells) bind to Jacalin and PNA, and these lectins are specific markers for type IV cells of the rat taste cells.
Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, Suita, Japan.
Ryo
R
Taniguchi
Lei
L
Shi
Masae
M
Fujii
Katsura
K
Ueda
Shiho
S
Honma
Satoshi
S
Wakisaka
D000818:Animals; D031622:Artocarpus; D015415:Biomarkers / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D019887:Peanut Agglutinin / Q000378:metabolism*; D037121:Plant Lectins / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013650:Taste Buds / Q000166:cytology* / Q000378:metabolism*; D015293:Transducin / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
D015415:Biomarkers; D019887:Peanut Agglutinin; D037121:Plant Lectins; C043696:jacalin; C075192:gustducin; C489034:UCHL1 protein, rat; D043222:Ubiquitin Thiolesterase; D015293:Transducin