Normal patterns of expression of glycosylphosphatidylinositol-anchored proteins on different subsets of peripheral blood cells: a frame of reference for the diagnosis of paroxysmal nocturnal hemoglobinuria.
BACKGROUND
Evaluation of the expression of glycosylphosphatidylinositol-anchored membrane proteins (GPI-AP) is currently used for the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH). In this study, we analyzed the amount of expression of a wide variety of GPI-AP in different subsets of hematopoietic cells present in normal peripheral blood (PB), to establish their normal patterns of expression and provide a frame of reference for the definition of the best combination of GPI-AP and PB cell subsets to be applied in the diagnosis and monitoring of PNH.


RESULTS
Our results show variable patterns of expression of different GPI-AP in distinct subsets of normal PB cells. Combined use of CD55 and CD59 represented the most useful dual-marker combination; however, its utility remained suboptimal for several subsets of leukocytes and for platelets.


CONCLUSIONS
For some cell subsets such as the neutrophils additional useful markers could be selected from a relatively broad panel (CD16/CD24/CD55/CD59/CD66b/CD157), whereas for other cell subsets the number of useful antigens was either restricted (monocytes: CD14/CD55/CD157; B cells: CD24/CD48/CD52/CD55; CD4+ T cells: CD48/CD52/CD55; eosinophils: CD55/CD59; CD8+ T cells: CD48/CD55) or limited to a single marker (CD48 on CD56low NK cells, CD55 on BDCA3- dendritic cells and CD56high NK cells, and CD59 for red cells), from all antigens analyzed.
Hernández-Campo
Pilar María
PM
Servicio General de Citometría, Centro de Investigación del Cáncer, Departamento de Medicina, Universidad de Salamanca, Salamanca, Spain.
Almeida
Julia
J
Sánchez
María Luz
ML
Malvezzi
Mariester
M
Orfao
Alberto
A
D015703:Antigens, CD / Q000097:blood; D000951:Antigens, Neoplasm / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000737:chemistry / Q000276:immunology; D000071178:CD48 Antigen; D000074301:CD52 Antigen; D018958:CD55 Antigens / Q000097:blood; D018968:CD58 Antigens / Q000097:blood; D018957:CD59 Antigens / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000097:blood; D017261:Glycosylphosphatidylinositols / Q000097:blood*; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000145:classification*; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood* / Q000175:diagnosis*; D006801:Humans; D016130:Immunophenotyping; D007962:Leukocytes / Q000737:chemistry / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012015:Reference Standards
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D015415:Biomarkers; D000071178:CD48 Antigen; C000604065:CD48 protein, human; D000074301:CD52 Antigen; C000613862:CD52 protein, human; D018958:CD55 Antigens; D018968:CD58 Antigens; D018957:CD59 Antigens; D006023:Glycoproteins; D017261:Glycosylphosphatidylinositols