Native protein sequences are designed to destabilize folding intermediates.
Hydrophobic core mutants of sperm whale apomyoglobin were constructed to investigate the amino acid sequence features that determine the folding properties. Replacements of all of the Ile residues with Leu and of all of the Ile and Val residues with Leu decreased the thermodynamic stability of the folded states against the unfolded states but increased the stability of the folding intermediates against the unfolded states, indicating that the amino acid composition of the protein core is important for the protein stability and folding cooperativity. To examine the effect of the arrangement of these hydrophobic residues, mutant proteins were further constructed: 12 sites out of the 18 Leu, 9 Ile, and 8 Val residues of the wild-type myoglobin were randomly replaced with each other so that the amino acid compositions were similar to that of the wild-type protein. Four mutant proteins were obtained without selection of the protein properties. These residue replacements similarly resulted in the stabilization of both the intermediate and folded states against the unfolded states, as compared to the wild-type protein. Thus, the arrangements of the hydrophobic residues in the native amino acid sequence are selected to destabilize the folding intermediate rather than to stabilize the folded state. The present results suggest that the two-state transition of protein folding or the transient formation of the unstable intermediate, which seems to be required for effective production of the functional proteins, has been a major driving force in the molecular evolution of natural globular proteins.
Biometal Science Laboratory, RIKEN Harima Institute, Mikazuki, Sayo, Hyogo 679-5148, Japan. yisogai@riken.jp
Yasuhiro
Y
Isogai
D000595:Amino Acid Sequence / Q000235:genetics*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D019791:Guanidine / Q000378:metabolism / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D009154:Mutation; D009211:Myoglobin / Q000737:chemistry / Q000235:genetics; D011487:Protein Conformation; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D050661:Sperm Whale / Q000235:genetics / Q000378:metabolism; D040681:Structural Homology, Protein; D013816:Thermodynamics
D009211:Myoglobin; D019791:Guanidine