Isomer-specific anti-obese and hypolipidemic properties of conjugated linoleic acid in obese OLETF rats.
Conjugated linoleic acid (CLA), a mixture of positional and geometric isomers of linoleic acid, has attracted considerable attention because of its potentially beneficial biologic effects both in vitro and in vivo. Our results clearly show the specific action of the 10trans,12cis-CLA isomer against hyperlipidemia and obesity in obese Otsuka Long-Evans Tokushima Fatty (OLETF) rats. After 2 weeks of feeding with 10t,12c-CLA, but not 9cis,11trans-CLA, abdominal adipose tissue weight and serum and hepatic lipid levels in OLETF rats were lower than those in linoleic acid-fed rats. These effects were attributable to suppressed fatty acid synthesis and enhanced fatty acid beta oxidation in the liver on a 10t,12c-CLA diet. Additionally, we showed that mRNA expression of fatty acid synthase, carnitine palmitoyltransferase, leptin, and sterol regulatory element binding protein-1 was also regulated by 10t,12c-CLA. We suppose that 10t,12c-CLA reveals hypolipidemic and anti-obese activity through the alteration of mRNA expressions in the liver and white adipose tissue.
Wang
Yu-Ming
YM
Department of Applied Biological Sciences, Saga University, Japan.
Nagao
Koji
K
Inoue
Nao
N
Ujino
Yoko
Y
Shimada
Yuji
Y
Nagao
Toshihiro
T
Iwata
Toshio
T
Kamegai
Takeshi
T
Yamauchi-Sato
Yoshie
Y
Yanagita
Teruyoshi
T
D000273:Adipose Tissue; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D006949:Hyperlipidemias / Q000178:diet therapy* / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D007536:Isomerism; D044243:Linoleic Acids, Conjugated / Q000737:chemistry* / Q000627:therapeutic use*; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000178:diet therapy* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D020334:Rats, Inbred OLETF
D001786:Blood Glucose; D007328:Insulin; D044243:Linoleic Acids, Conjugated; D012333:RNA, Messenger