Evaluation of binding affinity of protein-mutant DNA complexes in solution by laser spray mass spectrometry.
We have applied laser spray mass spectrometry developed by Hiraoka et al. to investigate the binding affinity of protein-mutant DNA complexes. The results were compared with our previous data of collision-induced dissociation (CID) experiments using electrospray ionization mass spectrometry (ESI-MS). Systematic experiments were carried out on the complexes of the c-Myb DNA binding domain (c-Myb DBD) bound to eight kinds of 16- or 22-mer point mutant double-stranded DNA (dsDNA), whose solution K(d) values are different in the range from 10(-9) M to 10(-7) M. The dissociation curve as a function of laser power was plotted for each complex, and the laser power where 50% of complex was dissociated (E(50%)) in population was obtained. The correlation coefficient between E(50%) and the relative binding free-energy change (DeltaDeltaG) of each complex formation in solutions was 0.9808, which is much better than the coefficient obtained by the previous ESI-CID experiments that was 0.859. In addition, complexes of the c-Myb DBD with five other mutant dsDNA were also examined to confirm that laser spray can be used to estimate the K(d) values of a DNA-protein complex in solutions if an appropriate calibration curve is available. In the process of laser spray, dissociations of these noncovalent complexes occur in solutions, but not in the gas phase. This differs greatly from ESI-CID. Laser spray mass spectrometry has been found to be better than ESI-CID in evaluating binding affinity of a protein to various mutant DNA.
International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Kanagawa, Japan.
Xiangguo
X
Shi
International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Kanagawa, Japan.
Yoshifumi
Y
Nishimura
International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Kanagawa, Japan. akashi@tsurumi.yokohama-cu.ac.jp.
Satoko
S
Akashi
Clean Energy Research Center, University of Yamanashi, Takeda-4, 400-8511, Kofu, Japan.
Atsushi
A
Takamizawa
Clean Energy Research Center, University of Yamanashi, Takeda-4, 400-8511, Kofu, Japan. hiraoka@ab11.yamanashi.ac.jp.
Kenzo
K
Hiraoka
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D007834:Lasers; D046911:Macromolecular Substances; D013058:Mass Spectrometry / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011786:Quality Control; D012492:Salts; D012680:Sensitivity and Specificity; D012996:Solutions; D013816:Thermodynamics
D046911:Macromolecular Substances; D011506:Proteins; D020598:Proto-Oncogene Proteins c-myb; D012492:Salts; D012996:Solutions; D004247:DNA