15-Lipoxygenase-1 induced by interleukin-4 mediates apoptosis in oral cavity cancer cells.
It is unknown whether IL-4 induces apoptosis in oral cavity cancer (OCC) cells and, if so, what the mediator of this apoptosis is. Therefore, this study investigated whether apoptosis of OCC cells is induced by IL-4 and whether 15-lipoxygenase-1 (15-LO-1), induced by IL-4, is the mediator of this apoptosis. SCC 1483 oral cavity cancer cells were used in these experiments, and flow cytometry and PARP cleavage were used to examine apoptosis. At an IL-4 dosage that inhibited 50% of cell proliferation, apoptosis was observed, and this apoptosis was inhibited by 2.2 microM caffeic acid (CA). 15-LO-1 mRNA expression was observed beginning at 8 h after treatment with IL-4, and apoptosis increased after 24 h treatment with IL-4. In this apoptosis, the caspase cascade was not involved. In summary, IL-4 induced apoptosis in SCC 1483 OCC cells, and 15-LO-1, induced by IL-4, may mediate this apoptotic pathway.
Department of Otorhinolaryngology, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemoon-gu, Seoul, Republic of Korea.
Jeong Hong
JH
Kim
Jung Hyun
JH
Chang
Joo-Heon
JH
Yoon
Jeung Gweon
JG
Lee
Jung Ho
JH
Bae
Kyung-Su
KS
Kim
D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001093:Arachidonate 15-Lipoxygenase / Q000235:genetics / Q000502:physiology*; D015153:Blotting, Western / Q000379:methods; D002109:Caffeic Acids / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000473:pathology*; D020169:Caspases / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D004306:Dose-Response Relationship, Immunologic; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D009062:Mouth Neoplasms / Q000201:enzymology / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014407:Tumor Cells, Cultured
D002109:Caffeic Acids; D012333:RNA, Messenger; D015847:Interleukin-4; C515716:ALOX15 protein, human; D001093:Arachidonate 15-Lipoxygenase; D020169:Caspases; C040048:caffeic acid