Laser-induced bubbles in living cells.
OBJECTIVE
Laser-activated micro- and nano-bubbles (LAB) in cells may be used as universal and sensitive non-toxic probes for measuring functional properties of individual cells.


METHODS
Such bubbles can be detected and imaged by microscopy and flow cytometry. LABs in living blood and tumor cells were induced by pulsed (532 nm, 10 nanoseconds) laser radiation and detected by the thermal lens optical method.


RESULTS
Registered lifetime and maximal diameter of the studied LABs varied within the ranges of 0.02-10 microseconds and 0.44-100 microm, respectively. LAB parameters, thresholds and probabilities, were found to depend upon the physiological state of cells. Specificity and sensitivity of LAB cytometry were increased due to the use of light-absorbing nanoparticles conjugated to specific monoclonal antibodies.


CONCLUSIONS
LAB were found to be the universal phenomena that can be used for sensitive and non-invasive monitoring of any individual cell, intact or nanoparticle-treated.
Laser Cytotechnology Laboratory, Luikov Heat and Mass Transfer Institute, Minsk 220072, Belarus. ld@hmti.ac.by
Dmitri O
DO
Lapotko
D002477:Cells / Q000000981:diagnostic imaging* / Q000187:drug effects; D005434:Flow Cytometry; D005740:Gases; D006801:Humans; D066298:In Vitro Techniques; D007834:Lasers; D008853:Microscopy; D008954:Models, Biological; D055095:Optics and Photonics; D010782:Photolysis / Q000187:drug effects / Q000528:radiation effects; D017319:Photosensitizing Agents / Q000494:pharmacology; D011859:Radiography; D014835:Volatilization / Q000187:drug effects / Q000528:radiation effects*
D005740:Gases; D017319:Photosensitizing Agents