Cloning and functional characterization of a fatty acid synthase component FAS2 gene from Saccharomyces kluyveri.
A gene coding the alpha subunit of fatty acid synthase (FAS2) was isolated from the budding yeast Saccharomyces kluyveri. Nucleotide sequence analysis indicated that this gene, termed Sk-FAS2, coded a protein having an amino acid sequence 83% identical to the FAS2 protein of S. cerevisiae (Sc-FAS2). The Sk-FAS2 gene was able to functionally complement an S. cerevisiae fas2 disruptant. This Sk-FAS2-expressing strain was found to produce larger amounts of C18 than C16, in contrast to the Sc-FAS2-expressing fas2 strain. In addition, fusion genes of Sk-FAS2 and Sc-FAS2 were transformed into a fas2-disrupted strain of S. cerevisiae, and fatty acid analysis of these transformants suggested that the region containing the acyl carrier protein and beta-ketoacyl reductase domains of yeast FAS2 protein play an important role in determining carbon chain length of fatty acids.
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B5 Nagatsuta, 226-8501, Yokohama, Kanagawa, Japan. toura@bio.titech.ac.jp
Takahiro
T
Oura
Susumu
S
Kajiwara
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics; D000213:Acyl Carrier Protein / Q000235:genetics; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D064429:Fatty Acid Synthases / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D005231:Fatty Acids, Unsaturated / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011487:Protein Conformation; D017434:Protein Structure, Tertiary / Q000235:genetics; D012440:Saccharomyces / Q000235:genetics* / Q000378:metabolism
D000213:Acyl Carrier Protein; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005656:Fungal Proteins; D015094:3-Hydroxyacyl CoA Dehydrogenases; D064429:Fatty Acid Synthases