[Effect of Aloe coarse polysaccharide on cytokine secretion of keratinocytes in vitro].
OBJECTIVE
To study the effects of Aloe coarse polysaccharide on the levels of growth factors (EGF, TGF-alpha, TGF-beta1) and interleukins (IL-1beta, IL-6, IL-8) and tumor necrosis factor (TNF) in cultured keratinocytes.


METHODS
The cultured keratinocytes were treated with Aloe coarse polysaccharide at concentrations of 75, 150, 300, 600, 1 200 mg x L(-1) land the equal volume of media as control group. The levels of EGF, TGF-alpha, TGF-beta1, IL-1beta, IL-6, IL-8 and TNF in the supernatants of cultured keratinocytes were assayed by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA).


RESULTS
Compared with the control group, the levels of EGF, TGF-alpha, IL-1beta, IL-6 and IL-8 were significantly increased by treatment with Aloe coarse polysaccharide (P < 0.05, P < 0.01) and in a dose dependent manner, and the levels of TGF-beta1 and TNF were also increased but no statistical significance.


CONCLUSIONS
Aloe coarse polysaccharide may promote keratinocytes to secrete EGF, TGF-alpha, IL-1beta, IL-6 and IL-8.
Provincial Burts Institute, Union Hospital, Fujian Medical University, Fuzhou 350001, China. fzxd-chen@sina.com
Xiao-Dong
XD
Chen
Bo-Yu
BY
Wu
Qiong
Q
Jiang
Li-Ying
LY
Huang
Zhong-Cheng
ZC
Wang
D000504:Aloe / Q000737:chemistry; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D016211:Transforming Growth Factor alpha / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D048069:Tumor Necrosis Factors / Q000378:metabolism
D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D011134:Polysaccharides; D016211:Transforming Growth Factor alpha; D053773:Transforming Growth Factor beta1; D048069:Tumor Necrosis Factors; D004815:Epidermal Growth Factor