[Effects of Astragalus membranaceus and Panax notoginseng on the transformation of bone marrow stem cells and proliferation of EPC in vitro].
OBJECTIVE
To investigate the effect and the possible mechanism underlying the promotional effect of Astragalus membranaceus and Panax notoginseng on the transformation of bone narrow stem cells and proliferation of EPC.


METHODS
The marrow blood was collected in the patients with ischemia of lower limbs and BM-MNCs were separated and proliferated under different conditions. A. morphologic observation was performed and the ratio of CD34+ cells was measured.


RESULTS
The shuttle shaped cells lined up as bunches with several round cells scattered. The ratio of CD34+ cells was significantly increased in groups treated with medium (P < 0.01) and lower (P < 0.05) dosages of A. membranaceus and medium (P < 0.01) and high dosages (P < 0.01) of P. notoginseng respectively as compared with control group.


CONCLUSIONS
A. membranaceus and P. notoginseng can promote the transformation and proliferation of EPC.
Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China. yangb5191@sina.com
Bo-hua
BH
Yang
Ling-qun
LQ
Zhu
Juan-zi
JZ
Zhang
Fu-ling
FL
Niu
Wei
W
Cui
D018952:Antigens, CD34 / Q000378:metabolism*; D027885:Astragalus propinquus / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D036145:Ginsenosides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D053723:Panax notoginseng / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
D018952:Antigens, CD34; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides