Acetylsalicylic acid is compounding to antiplatelet effect of C-reactive protein.
The contribution of inflammatory process to the modulation of platelet response to acetylsalicylic acid (ASA) remains obscure. In our study, we examined the in vitro effect of C-reactive protein (CRP) on the ASA-mediated inhibition of collagen-stimulated platelet reactivity. Influence of CRP on platelet responsiveness to ASA was analysed using classical turbidimetric aggregation and flow cytometry. When acting alone, both C-reactive protein and ASA inhibited collagen-dependent platelet aggregation and reduced the expressions of two platelet surface membrane activation markers: P-selectin and activated GPIIbIIIa complex. Compared to the effects observed for ASA alone, the simultaneous action of both agents lead to further reductions in platelet aggregation (by 56.7+/-1.0% vs. 14.9+/-0.6%, p<0.0001) and lowered the expressions of platelet surface membrane P-selectin (by 72.1+/-5.3% vs. 65.0+/-6.0%, p<0.01) and activated GPIIbIIIa (by 67.0+/-5.6% vs. 47.7+/-8.3%, p<0.01). In general, our findings showed for the first time the augmenting effect of native C-reactive protein in the antiplatelet action of acetylsalicylic acid. Thus, we conclude that the effectiveness of aspirin therapy may strongly depend upon the presence of native CRP in circulation.
Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Medical University Hospital No. 2, 113 Zeromskiego street, 90-549 Lodz, Poland.
Magdalena
M
Boncler
Boguslawa
B
Luzak
Marcin
M
Rozalski
Jacek
J
Golanski
Blazej
B
Rychlik
Cezary
C
Watala
D000328:Adult; D001241:Aspirin / Q000494:pharmacology*; D002097:C-Reactive Protein / Q000494:pharmacology*; D002478:Cells, Cultured; D004357:Drug Synergism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D009391:Nephelometry and Turbidimetry; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis
D019007:P-Selectin; D010975:Platelet Aggregation Inhibitors; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D002097:C-Reactive Protein; D001241:Aspirin