Multistrain genetic comparisons reveal CCR5 as a receptor involved in airway hyperresponsiveness.
Asthma is a ubiquitous disease with a broad range of clinical phenotypes. To better understand the complex genetic and environmental interactions underlying asthma, we compared the gene-gene interactions of four genetically distinct mouse strains that demonstrate biologically distinct responses to allergen. Using DNA microarrays and knock-out mouse studies, we showed that CCR5 plays a definitive role in the development of ovalbumin-induced allergic airway inflammatory disease. In addition, gene expression profiling data have revealed other potential novel targets for therapeutics-based research and has enhanced the understanding of the molecular mechanisms underlying the etiology of "asthma."
Walker
Julia K L
JK
Department of Pulmonary, Allergy, and Critical Care Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA. walke082@mc.duke.edu
Ahumada
Adriana
A
Frank
Bryan
B
Gaspard
Renee
R
Berman
Katherine
K
Quackenbush
John
J
Schwartz
David A
DA
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D018893:Bronchoalveolar Lavage; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D010047:Ovalbumin; D010641:Phenotype; D019713:Receptors, CCR5 / Q000235:genetics* / Q000378:metabolism*; D012130:Respiratory Hypersensitivity / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism*
D019713:Receptors, CCR5; D010047:Ovalbumin