Oxidative DNA damage induced by autoxidation of microquantities of S(IV) in the presence of Ni(II)-Gly-Gly-His.
NiIIGGH (GGH = glycylglycylhistidine) reacts rapidly with S(IV), in air-saturated solution, to produce NiIIIGGH. A mechanism is proposed where initial NiIII oxidizes SO3(2-) to SO3*-, which reacts with dissolved oxygen to produce SO5*-, initiating radical chain reactions. DNA strand breaks and 8-oxo-7,8-dihydro-2'-deoxyguanosine formation were observed in air-saturated solutions containing micromolar concentrations of Ni(II) and S(IV). The extent of DNA damage showed dependence on the ratio of the NiIIGGH : S(IV) concentrations and the ionic strength.
Instituto de Química, Universidade de São Paulo, SP, Brazil.
María V
MV
Alipázaga
Ruben G M
RG
Moreno
Edlaine
E
Linares
Marisa H G
MH
Medeiros
Nina
N
Coichev
D000080242:8-Hydroxy-2'-Deoxyguanosine; D001665:Binding Sites; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000187:drug effects; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005609:Free Radicals; D007202:Indicators and Reagents; D009842:Oligopeptides / Q000737:chemistry*; D010084:Oxidation-Reduction; D010957:Plasmids / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D019097:Spin Trapping; D013455:Sulfur / Q000737:chemistry* / Q000633:toxicity*
D005609:Free Radicals; D007202:Indicators and Reagents; D009842:Oligopeptides; D013455:Sulfur; C037026:diglycyl-histidine; D003300:Copper; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine