Development of a direct species-specific PCR assay for differential diagnosis of Leishmania tropica.
We have developed a polymerase chain reaction assay for differential diagnosis of Leishmania tropica, based on simple amplification of the target region. The assay detects less than 5 protozoan cells, was tested on human samples and an experimentally infected animal, and is appropriate for clinical laboratories in countries where leishmaniasis is endemic.
Institute of Parasitology, Czech Academy of Sciences, 37005 Ceské Budejovice, Czech Republic.
Milan
M
Jirkù
Eva
E
Zemanová
Amer
A
Al-Jawabreh
Gabriele
G
Schönian
Julius
J
Lukes
D000328:Adult; D000818:Animals; D002648:Child; D002675:Child, Preschool; D006224:Cricetinae; D016054:DNA, Protozoan / Q000032:analysis; D003937:Diagnosis, Differential; D006801:Humans; D007895:Leishmania tropica / Q000235:genetics* / Q000302:isolation & purification; D007896:Leishmaniasis / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D008647:Mesocricetus; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity
D016054:DNA, Protozoan