A 2-year retrospective analysis of laboratory testing for activated protein C resistance with a factor V-corrected activated partial thromboplastin time-based method.
The factor V-corrected activated protein C resistance assay is the test of choice to screen for the factor V Leiden mutation. During the past 2 years, local test results with the frequently used Coatest APCR kit were evaluated and compared with the results of DNA analysis, the 'gold standard'. Samples of 278 patients were analysed by both techniques. We were unable to confirm that factor V Leiden carriers can clearly be delineated from normal individuals with the Coatest APCR test. A ratio of 2.0 as the cut-off provides 99.0% sensitivity and 95.4% specificity.To evaluate the lupus anticoagulant interference, we retrospectively analysed 16 lupus anticoagulant-positive patients. In this study, two (12.5%) showed a false-positive activated protein C resistance result. Six out of 16 (37.5%) lupus anticoagulant-positive patients were also carriers of the factor V Leiden mutation. Four out of eight (50%) false-positive activated protein C resistance results presented with an abnormal baseline clotting time. In order to prevent reporting false-positive results, a maximum baseline clotting time (65.8 s) was calculated. A new scheme for interpreting activated protein C resistance ratios was proposed.
Laboratory of Clinical Biology, Ghent University Hospital, Ghent, Belgium.
Annelies V
AV
De Bel
Greta A
GA
Van der Cruyssen
Katrien M
KM
Devreese
D020016:Activated Protein C Resistance / Q000097:blood* / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods; D005165:Factor V / Q000032:analysis* / Q000235:genetics; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D016682:Lupus Coagulation Inhibitor / Q000097:blood* / Q000235:genetics; D008297:Male; D008403:Mass Screening / Q000379:methods; D010314:Partial Thromboplastin Time / Q000379:methods; D011237:Predictive Value of Tests
D016682:Lupus Coagulation Inhibitor; C095381:factor V Leiden; D005165:Factor V