Electrophoresis of DNA in human genetic diagnostics - state-of-the-art, alternatives and future prospects.
Electrophoretic separation of nucleic acids according to their molecular weights has dominated the methods' spectrum in molecular genetics for nearly half a century. We review the current methodological basis and evaluate its impact with special reference to new developments in the microarray technology. Although electrophoresis may be made redundant for many applications in DNA diagnostics within a few years, a number of electrophoretic vestiges will remain irreplaceable in the foreseeable future.
Department of Human Genetics, Ruhr-University, Bochum, Germany. rene.goedde@rub.de
René
R
Gödde
Denis-Amer
DA
Akkad
Larissa
L
Arning
Gabriele
G
Dekomien
Julia
J
Herchenbach
Erdmute
E
Kunstmann
Moritz
M
Meins
Stefan
S
Wieczorek
Jörg T
JT
Epplen
Sabine
S
Hoffjan
D015152:Blotting, Northern; D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods* / Q000639:trends; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary; D006801:Humans; D046210:Microfluidic Analytical Techniques; D018895:Microsatellite Repeats; D020411:Oligonucleotide Array Sequence Analysis; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
D004247:DNA