[Effect of different parts of Portulace oleracea on the levels of TNF-alpha and IL-6 in the supernatant of cultured adipose cell].
OBJECTIVE
To explore the effects of drug-carried serum of the different parts of Portulace olerace on cytokine TNF-alpha and IL-6 secreted by adipose cell in vitro.


METHODS
Models of adipose cell were established by Rodbell method. Using the method of seropharmacology, the drug-carried serum of the different parts of P. olerace were prepared. The cell viability of each group was tested by. Methy thiazolyl tetrazolium (MTT) assay. The levels of TNF-alpha and IL-6 in the supernatant of cultured adipose cell were assayed by RIA.


RESULTS
MTT assay results showed the cell viability of normal serum group was significantly higher than that of high lipid serum( P < 0.05). Compared with the high lipid serum group, the cell viability of the drug-carried serum groups in 40% and 20% concentration were significantly increased( P < 0.05). The high lipid serum had a better effect on increasing the levels of TNF-alpha and IL-6 than the normal serum group (P < 0.01). Expect the drug-carried serum of P. olerace low dose group in 20% concentration, each drug-carried serum group could markedly lower the levels of TNF-alpha (P < 0.05 or P < 0.01). Each drug-carried serum group in 40% concentration and the drug-carried serum P. olerace MS high dose group in 20% concentration could markedly lower the levels of IL-6 (P < 0.05 or P < 0.01).


CONCLUSIONS
The drug-carried serum of P. olerace and its different parts act on adipose cell damaged by the high lipid serum, significantly increas the cell viability in the groups in 40% and 20% concentration, and improve the disorder of lipid in differeut degrel by lowering the levels of TNF-alpha and IL-6 that adipose cell secreted in vitro.
Tongji Hospital, Tongii Medical College, Huazhong University of Science & Technology, Wuhan 430030, China. xiaofengyinglh@sina.com.cn
Feng-ying
FY
Xiao
Fu-er
FE
Lu
Li-jun
LJ
Xu
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D047309:Flavones / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D006949:Hyperlipidemias / Q000097:blood; D015850:Interleukin-6 / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D031822:Portulaca / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D044967:Serum / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D005231:Fatty Acids, Unsaturated; D047309:Flavones; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha