[Expression changes of interleukin-1 receptor associated kinase-4 during endotoxin tolerance development in kupffer cells].
OBJECTIVE
To explore the mechanism of endotoxin tolerance (ET) through observing the expression of interleukin 1 receptor associated kinase-4 (IRAK-4) during endotoxin tolerance development in Kupffer cells (KCs).


METHODS
Isolated KCs of Balb/c mouse were divided into two groups: the non-endotoxin tolerance (NET) group and the endotoxin tolerance (ET) group, which were pretreated with 10 ng/ml lipopolysaccharide (LPS) for 24 h. Then, the two groups were treated with 100 ng/ml LPS. The expressions of IRAK-4 gene and protein level were determined by RT-PCR and Western blot. The activities of NF-kappaB of KCs and the TNFalpha level were estimated by ELISA at 0 h, 1 h, 3 h, 6 h and 12 h after LPS stimulation.


RESULTS
The ultimate level of IRAK-4, the activities of NF-kappaB and the TNFalpha level were evidently lower in the ET group than those in the NET group (t = 12.4, 17.4 and 138.9 respectively, P<0.01).


CONCLUSIONS
Pretreatment with LPS on KCs could induce endotoxin tolerance of KCs and inhibition of IRAK-4 expression may be one of the reasons for its development.
Li
Sheng-wei
SW
Department of Hepatobiliary Surgery, Second Affiliated Hospital of Chongqing of Medical Sciences University, Chongqing 400010, China.
Liu
Zuo-jin
ZJ
Liu
Chang-an
CA
Li
Xu-hong
XH
You
Hai-bo
HB
Chen
Xian-feng
XF
Gong
Jian-ping
JP
D000818:Animals; D002478:Cells, Cultured; D004731:Endotoxins / Q000276:immunology*; D007108:Immune Tolerance; D053592:Interleukin-1 Receptor-Associated Kinases / Q000096:biosynthesis* / Q000235:genetics; D007728:Kupffer Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D004731:Endotoxins; D008070:Lipopolysaccharides; C507773:IRAK4 protein, human; D053592:Interleukin-1 Receptor-Associated Kinases