Apparent role of dynein in glucose-6-phosphate dehydrogenase trafficking in neutrophils from pregnant women.
To better understand the mechanisms of metabolic microcompartmentalization associated with neutrophil hexose monophosphate shunt activity during pregnancy, we have studied the intracellular trafficking of glucose-6-phosphate dehydrogenase (G6PDase). Microtubule motor proteins colocalize with G6PDase. Dynein inhibitors block G6PDase accumulation at the microtubule-organizing center in pregnancy cells. On this basis, we conclude that microtubule motor proteins participate in hexose monophosphate shunt enzyme transport within leukocytes.
Huang
Ji-Biao
JB
Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI 48105, USA.
Espinoza
Jimmy
J
Romero
Roberto
R
Petty
Howard R
HR
D002451:Cell Compartmentation; D004398:Dyneins / Q000502:physiology*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D008868:Microtubule Proteins / Q000378:metabolism; D020409:Molecular Motor Proteins / Q000378:metabolism; D009504:Neutrophils / Q000737:chemistry / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D011247:Pregnancy / Q000378:metabolism*; D021381:Protein Transport
D008868:Microtubule Proteins; D020409:Molecular Motor Proteins; D005954:Glucosephosphate Dehydrogenase; D004398:Dyneins