Analysis of GABRB2 association with schizophrenia in German population with DNA sequencing and one-label extension method for SNP genotyping.
OBJECTIVE
Schizophrenia (SCZ) is a complex mental disease that affects approximately 1% of the population. In this study, six SNPs in GABRB2 were genotyped for a case-control association study with the cycloid psychosis subtype of SCZ in the German population using two methods for SNP genotyping.


METHODS
The SNPs were genotyped by direct DNA sequencing, as well as a novel one-label extension method. The results were analyzed for association with SCZ.


CONCLUSIONS
Significant association was found for SNPs rs1816071 and rs1816072 with SCZ susceptibility. This is consistent with our previous finding of association of SNPs in GABRB2 with SCZ susceptibility in Han Chinese. There was a total agreement between the genotyping results from one-label extensions and the results of direct DNA sequencing, thus validating the accuracy of the one-label extension method of SNP genotyping.
Department of Biochemistry and Applied Genomics Laboratory, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Zhiliang
Z
Yu
Jianhuan
J
Chen
Haifeng
H
Shi
Gerald
G
Stoeber
Shui-Ying
SY
Tsang
Hong
H
Xue
D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D004198:Disease Susceptibility; D005838:Genotype; D005858:Germany; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011963:Receptors, GABA-A / Q000235:genetics*; D012559:Schizophrenia / Q000208:ethnology / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013698:Templates, Genetic; D044465:White People / Q000235:genetics*
C495710:GABRB2 protein, human; D011963:Receptors, GABA-A