[The effect of serum rich in platelet-released growth factors on proliferation of rat osteoblast in vitro].
OBJECTIVE
To study the effect of serum rich in platlelet-released growth factors on the biological functions of rat osteoblast.


METHODS
Rat osteoblasts were isolated from parietal bone of fetal Spraque-Dawley (SD) rat. Platelet-rich plasma(PRP) and platelet-poor plasma (PPP) obtained from healthy SD rat were activated by thrombin to get serum rich in growth factors (SRGF) or serum poor in growth factors (SPGF). The cells were treated with 1.25%, 2.5%, 5% SRGF or SPGF, and cellular mitogenic activity was evaluated by thiazoly blue (MTT) colorimetric assay. Results When compared with SPGF at the same concentration, SRGF promoted the proliferation of rat osteoblast significantly (P = 0.002 - 0.004), and the proliferation seemed to correlate with the concentration of SRGF (Spearman's correlation coefficient, r(s) = 0.834).


CONCLUSIONS
SRGF is capable of upregulating the proliferation of rat osteoblast.
Department of Prosthodontics, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Yang
Y
Song
Ping
P
Gong
Lei
L
Wang
Yon-lie
YL
Chao
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010294:Parietal Bone / Q000166:cytology; D010982:Platelet-Derived Growth Factor / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum / Q000737:chemistry
D003470:Culture Media; D010982:Platelet-Derived Growth Factor