Analytical separation and detection methods for flavonoids.
Flavonoids receive considerable attention in the literature, specifically because of their biological and physiological importance. This review focuses on separation and detection methods for flavonoids and their application to plants, food, drinks and biological fluids. The topics that will be discussed are sample treatment, column liquid chromatography (LC), but also methods such as gas chromatography (GC), capillary electrophoresis (CE) and thin-layer chromatography (TLC), various detection methods and structural characterization. Because of the increasing interest in structure elucidation of flavonoids, special attention will be devoted to the use of tandem-mass spectrometric (MS/MS) techniques for the characterization of several important sub-classes, and to the potential of combined diode-array UV (DAD UV), tandem-MS and nuclear magnetic resonance (NMR) detection for unambiguous identification. Emphasis will be on recent developments and trends.
Quest International, Department of Analytical Research and Development, Huizerstraatweg 28, 1411 GP Naarden, The Netherlands. drijke@few.vu.nl
Eva
E
de Rijke
Pieter
P
Out
Wilfried M A
WM
Niessen
Freek
F
Ariese
Cees
C
Gooijer
Udo A Th
UA
Brinkman
D001628:Beverages / Q000032:analysis; D005591:Chemical Fractionation / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D005504:Food Analysis; D013058:Mass Spectrometry / Q000379:methods; D010944:Plants / Q000737:chemistry
D005419:Flavonoids