[Studies on the process of Herba Clinopodii saponins purified with macroporous adsorption resin].
OBJECTIVE
To study the technological parameters of the purification process of saponins with macroporous adsorption resin.


METHODS
The adsorptive characteristics and elutive parameters of the process were studied by taking the elutive and purified ratio of saponins as markers.


RESULTS
11.4 ml of the extraction of Herba Clinopodii (crude drugs 0.2 g/ml) was purified with a column of macroporous adsorption resin (phi15 mm x H90 mm, dry weight 2.5 g) and washed with 3BV of distilled water, then eluted with 3BV of 30% ethanol and 3BV of 70% ethanol. Most of saponins were collected in the 70% ethanol.


CONCLUSIONS
With macroporous adsorption resin adsorbing and purifying,the elutive ratio of saponins is 86.8% and the purity reaches 153.2%. So this process of applying macroporous adsorption resin to adsorb and purify Saponins is feasible.
Zhang
Yi
Y
Chongqing City Institute for Drug Control, Chongqing 400015.
Yan
Dan
D
Han
Yumei
Y
D000327:Adsorption; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol; D019686:Lamiaceae / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D014867:Water
D004365:Drugs, Chinese Herbal; D012117:Resins, Synthetic; D012503:Saponins; D014867:Water; D000431:Ethanol