Porphyromonas gingivalis gingipains mediate the shedding of syndecan-1 from the surface of gingival epithelial cells.
Porphyromonas gingivalis gingipains are thought to be critical virulence factors in periodontitis. Increased serum levels of the soluble ectodomains of surface effectors have been reported to occur during bacterial infections. In the present study, we show that the cell surface proteoglycan syndecan-1 was highly expressed on human gingival epithelial cells. Treatments with P. gingivalis culture supernatants consistently mediated the shedding of syndecan-1 from the surface of epithelial cells. Concomitantly, the amount of soluble syndecan-1 detected in the culture medium increased significantly in a time-dependent manner. However, neither a heat-inactivated supernatant nor a supernatant from a gingipain-deficient mutant had a significant effect on syndecan-1 shedding. Such a shedding process may play an important role in the bacterial invasion of periodontal tissue and the modulation of host defences.
Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada.
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E
Andrian
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D
Grenier
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Rouabhia
D018829:Adhesins, Bacterial / Q000494:pharmacology*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D003546:Cysteine Endopeptidases / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D000080867:Gingipain Cysteine Endopeptidases; D005881:Gingiva / Q000187:drug effects* / Q000473:pathology; D006388:Hemagglutinins / Q000494:pharmacology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis / Q000187:drug effects*; D016966:Porphyromonas gingivalis / Q000502:physiology*; D011509:Proteoglycans / Q000032:analysis / Q000187:drug effects*; D053668:Syndecan-1; D053667:Syndecans; D013997:Time Factors; D037521:Virulence Factors / Q000494:pharmacology
D018829:Adhesins, Bacterial; D017077:Culture Media, Conditioned; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508241:SDC1 protein, human; D053668:Syndecan-1; D053667:Syndecans; D037521:Virulence Factors; D003546:Cysteine Endopeptidases