[Comparison of supercritical fluid extraction and steam distillation methods for the extraction of essential oils from Schizonepeta tenuifolia Briq].
Essential oil was extracted from Schizonepeta tenuifolia Briq. by supercritical fluid extraction (SFE) and steam distillation (SD). The components extracted were determined by gas chromatography with area normalization method and identified by gas chromatography-mass spectrometry (GC-MS). The optimal chromatographic conditions were: capillary column, SE-54 (30 m x 0.25 mm i.d., 0.25 microm); column temperature, 50 degrees C (3 min) --> (5 degrees c/min) 180 degrees C (2 min --> (10 degrees C/ min) 260 degrees C 50 min); split injection, split ratio 1: 50; injector temperature, 280 degrees C. Fifty-four components were identified for the essential oils extracted by SFE, and its main components were found to be pulegone, menthone, linoleic acid chloride etc. Thirty-nine components were identified for the essential oil obtained by SD, and its main components were found to be pulegone, menthone, limonene etc. The SFE method is better than the SD method in reliability stability and reproducibility, and is thus well suitable for similar applications involving for extraction of other traditional Chinese herbal medicines.
Qiu
Qin
Q
School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
Ling
Jianya
J
Ding
Yuping
Y
Chang
Hongwen
H
Wang
Jiang
J
Liu
Tingli
T
D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D000081005:Cyclohexane Monoterpenes; D057168:Distillation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D019686:Lamiaceae / Q000737:chemistry*; D019787:Linoleic Acid / Q000737:chemistry / Q000302:isolation & purification; D008610:Menthol / Q000737:chemistry / Q000302:isolation & purification; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification*; D015203:Reproducibility of Results
D000081005:Cyclohexane Monoterpenes; D004365:Drugs, Chinese Herbal; D039821:Monoterpenes; D009822:Oils, Volatile; D008610:Menthol; C039648:pulegone; D019787:Linoleic Acid; C019466:menthone