Intracellular Mg2+ influences both open and closed times of a native Ca2+-activated BK channel in cultured human renal proximal tubule cells.
Effects of intracellular Mg2+ on a native Ca(2+)-and voltage-sensitive large-conductance K+ channel in cultured human renal proximal tubule cells were examined with the patch-clamp technique in the inside-out mode. At an intracellular concentration of Ca2+ ([Ca2+](i)) of 10(-5)-10(-4) M, addition of 1-10 mM: Mg2+ increased the open probability (P(o)) of the channel, which shifted the P(o) -membrane potential (V(m)) relationship to the negative voltage direction without causing an appreciable change in the gating charge (Boltzmann constant). However, the Mg(2+)-induced increase in P(o) was suppressed at a relatively low [Ca2+](i) (10(-5.5)-10(-6) M). Dwell-time histograms have revealed that addition of Mg2+ mainly increased P(o) by extending open times at 10(-5) M Ca2+ and extending both open and closed times simultaneously at 10(-5.5) M Ca2+. Since our data showed that raising the [Ca2+](i) from 10(-5) to 10(-4) M increased P(o) mainly by shortening the closed time, extension of the closed time at 10(-5.5) M Ca(2+) would result from the Mg(2+)-inhibited Ca(2+)-dependent activation. At a constant V(m), adding Mg2+ enhanced the sigmoidicity of the P(o)-[Ca2+](i) relationship with an increase in the Hill coefficient. These results suggest that the major action of Mg2+ on this channel is to elevate P(o) by lengthening the open time, while extension of the closed time at a relatively low [Ca2+](i) results from a lowering of the sensitivity to Ca2+ of the channel by Mg2+, which causes the increase in the Hill coefficient.
Department of Physiology II, School of Medicine, Iwate Medical University, 19-1, Uchimaru, Morioka, 020-8505, Japan. mkubokaw@iwate-med.ac.jg
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D002118:Calcium / Q000737:chemistry*; D002413:Cations, Divalent; D002478:Cells, Cultured; D006801:Humans; D007424:Intracellular Fluid / Q000737:chemistry / Q000378:metabolism*; D015640:Ion Channel Gating / Q000502:physiology; D007687:Kidney Tubules, Proximal / Q000737:chemistry / Q000166:cytology* / Q000502:physiology*; D007700:Kinetics; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000737:chemistry / Q000502:physiology*; D008274:Magnesium / Q000737:chemistry*; D008564:Membrane Potentials / Q000502:physiology; D018408:Patch-Clamp Techniques; D013997:Time Factors
D002413:Cations, Divalent; D051036:Large-Conductance Calcium-Activated Potassium Channels; D008274:Magnesium; D002118:Calcium