Eph/Ephrin membrane proteins: a mammalian expression vector pTIg-BOS-Fc allowing rapid protein purification.
There is an urgent need for high purity, single chain, fully functional Eph/ephrin membrane proteins. This report outlines the pTIg-BOS-Fc vector and purification approach resulting in rapid increased production of fully functional single chain extracellular proteins that were isolated with high purity and used in structure-function analysis and pre-clinical studies.
Leukaemia Foundation of Queensland Laboratory, Queensland Institute of Medical Research. P.O Royal Brisbane Hospital, Queensland 4029, Australia. Bryan.Day@qimr.edu.au
Bryan W
BW
Day
Fiona M
FM
Smith
Ke
K
Chen
Jennifer K
JK
McCarron
Nirmitha I
NI
Herath
Martin
M
Lackmann
Andrew W
AW
Boyd
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D036342:Ephrins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D015870:Gene Expression / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D013997:Time Factors
D036342:Ephrins; D007141:Immunoglobulin Fc Fragments