Proteome analysis of rice root proteins regulated by gibberellin.
To gain an enhanced understanding of the mechanism by which gibberellins (GAs) regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, effective, and reliable tool in differential protein expressions. In previous studies, sixteen proteins showed differences in accumulation levels as a result of treatment with GA(3), uniconazole, or abscisic acid (ABA), and/or the differences between the GA-deficient semi-dwarf mutant, Tan-ginbozu, and normal cultivars. Among these proteins, aldolase increased in roots treated with GA(3), was present at low levels in Tan-ginbozu roots, and decreased in roots treated with uniconazole or ABA. In a root elongation assay, the growth of aldolase-antisense transgenic rice was half of that of vector control transgenic rice. These results indicate that increases in aldolase activity stimulate the glycolytic pathway and may play an important role in the GA-induced growth of roots. In this review, we discuss the relationship among GA, aldolase, and root growth.
Komatsu
Setsuko
S
Department of Molecular Genetics, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan. skomatsu@affrc.go.jp
Konishi
Hirosato
H
D002118:Calcium / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics / Q000378:metabolism; D005875:Gibberellins / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D002118:Calcium; D005634:Fructose-Bisphosphate Aldolase; D005875:Gibberellins; D005947:Glucose; D010940:Plant Proteins; D011494:Protein Kinases; D020543:Proteome; C454865:calcium-dependent protein kinase, rice; D017871:Calcium-Calmodulin-Dependent Protein Kinases