Qualitative and quantitative determination of extractives in heartwood of Scots pine (Pinus sylvestris L.) by gas chromatography.
A method for quantitative determination of extractives from heartwood of Scots pine (Pinus sylvestris L.) using gas chromatography (GC) with flame ionization detection (FID) was developed. The limit of detection (LOD) was 0.03 mg/g wood and the linear range (r = 0.9994) was up to 10 mg/g with accuracy within +/- 10% and precision of 18% relative standard deviation. The identification of the extractives was performed using gas chromatography combined with mass spectrometry (GC-MS). The yields of extraction by Soxhlet were tested for solid wood, small particles and fine powder. Small particles were chosen for further analysis. This treatment gave good yields of the most important extractives: pinosylvin, pinosylvin monomethyl ether, resin acids and free fatty acids. The method is used to demonstrate the variation of these extractives across stems and differences in north-south direction.
University of Life Sciences, Department of Chemistry, Biotechnology and Food Science, P.O. Box 5003, N-1432 As, Norway. dag.ekeberg@umb.no
Dag
D
Ekeberg
Per-Otto
PO
Flaete
Morten
M
Eikenes
Monica
M
Fongen
Carl Fredrik
CF
Naess-Andresen
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results
D010936:Plant Extracts