SECRET AGENT and SPINDLY have overlapping roles in the development of Arabidopsis thaliana L. Heyn.
O-GlcNAc transferase (OGT) catalyses transfer of GlcNAc (N-acetylglucosamine) to serine or threonine of proteins. The Arabidopsis OGTs, SECRET AGENT (SEC) and SPINDLY (SPY) have overlapping functions during gametogenesis and embryogenesis. SPY functions in a number of processes including circadian, light, and gibberellin (GA) responses. The role of SEC in plant development and GA signalling was investigated by determining the phenotypes of sec-1 and sec-2 plants and the expression pattern of SEC. Similar to SPY, SEC transcripts were ubiquitous. Although there is no evidence of transcript-level regulation by other factors, SEC mRNA levels are elevated in spy plants and SPY mRNA levels are elevated in sec plants. sec-1 and sec-2 plants exhibited few of the defects observed in spy plants and had wild-type GA responses. Compared with wild type, sec plants produced leaves at a reduced rate. Haplo-insufficiency at SEC in a spy ga1 double mutant background suppressed spy during germination and enhanced the production of ovaries with four carpels by spy. By contrast, SPY haplo-insufficiency in a sec ga1 double mutant background caused a novel phenotype, production of a proliferation of pin-like structures instead of a floral shoot. These results are consistent with SEC function overlapping with SPY for leaf production and reproductive development.
Department of Plant Biology, University of Minnesota, 250 Biological Sciences Center, 1445 Gortner Ave., St Paul, 55108, USA.
Lynn M
LM
Hartweck
Ruth K
RK
Genger
William M
WM
Grey
Neil E
NE
Olszewski
D017360:Arabidopsis / Q000033:anatomy & histology / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D005875:Gibberellins / Q000378:metabolism; D007113:Immunity, Innate / Q000502:physiology; D018519:Meristem / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D009154:Mutation; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D018515:Plant Leaves / Q000254:growth & development; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*
D029681:Arabidopsis Proteins; D005875:Gibberellins; D012097:Repressor Proteins; C101465:SPY protein, Arabidopsis; C007842:gibberellic acid; D017351:N-Acetylglucosaminyltransferases; C510668:SECRET AGENT protein, Arabidopsis