[Proteomics technology for screening biomarkers of oligospermic candidates].
OBJECTIVE
To screening specific biomarkers of oligospermic candidates from seminal plasma by proteomics technology.


METHODS
The protein spectra of 20 seminal plasma from oligospermic patients and healthy male individuals were analyzed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) to compare different protein expressions.


RESULTS
Compared with normal control group, a specific protein peak of molecular weight 11 022.9 in the protein spectra of oligospermic patients'semen on H4 protein-chip reduced significantly, but the protein peak of molecular weight 11 507.4 elevated apparently. For SAX-2 protein-chip, a specific protein peak of molecular weight 16 751.6 in the protein spectra of oligospermic patients' semen elevated significantly.


CONCLUSIONS
Screening specific proteins in seminal plasma of oligospermic patients is important for understanding the mechanism of oligospermia and screening suspected oligospermic individuals.
Yang
Huan
H
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China.
Zhang
Jie
J
Zhang
Wei
W
Long
Wen
W
Zhang
Xiao-bin
XB
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000175:diagnosis*; D040081:Protein Array Analysis; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012661:Semen / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D015415:Biomarkers; D011506:Proteins