[Analysis of saponins from panax notoginseng using pressurized solvent extraction coupled with liquid chromatography-electrospray mass spectrum].
OBJECTIVE
To establish a method for qualitative analysis of saponins from Panax notoginseng using pressurized solvent extraction coupled with LC-ESI-MS.


METHODS
The PSE technology was applied to the process of extraction for Panax notoginseng, and the negative ion detection and multiple reaction monitoring model were used. The saponins were investigated based on total ion chromatogram (TIC) and MRM chromatogram.


RESULTS
According to the fragment character of saponins, the molecular weight and their structures could be identified.


CONCLUSIONS
The method can be used for qualitative analysis of saponins from Panax notoginseng.
Institute of Chinese Medical Sciences, University of Macau, Macau SAR.
Jianbo
J
Wan
Changming
C
Li
Shaopin
S
Li
Lingyi
L
Kong
Yitao
Y
Wang
D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D008970:Molecular Weight; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011312:Pressure; D011786:Quality Control; D012503:Saponins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization; D013678:Technology, Pharmaceutical / Q000379:methods
D036145:Ginsenosides; D012503:Saponins; D012997:Solvents