[Genetically modified industrial brewing yeast with high-glutathione and low-diacetyl production].
Recombinant plasmid pICG was constructed by replacing the internal fragment of a-acetohydroxyacid synthase (AHAS) gene (ILV2) with a copy of gamma-glutamylcysteine synthetase gene (GSH1) and copper chelatin gene (CUP1) from the industrial brewing yeast strain YSF31. YSF31 was transformed with plasmid pICG linearized by Kpn I and Pst I. A recombinant strain with high-glutathione and low-diacetyl production was selected. The results of fermentation in 100-L bioreactor showed that the lagering time of beer produced for recombinant strain T2 was shortened by 3 days and the shelf life of the beer was prolonged about 50%. It may be more acceptable for the commercial application, as it does not contain foreign DNA.
The Laboratory of Molecular Genetics and Breeding of Yeast, Institute of Microbiology of Chinese Academy of Sciences, Beijing, 100080, China.
Ji-Na
JN
Zhang
Xiu-Ping
XP
He
Xue-Na
XN
Guo
Nan
N
Liu
Bo-Run
BR
Zhang
D000095:Acetolactate Synthase / Q000235:genetics / Q000378:metabolism; D001515:Beer / Q000382:microbiology*; D003001:Cloning, Molecular; D003931:Diacetyl / Q000378:metabolism*; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis*; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism; D030781:Organisms, Genetically Modified / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
C500769:CUP1-1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D008668:Metallothionein; D000095:Acetolactate Synthase; C490862:GSH1 protein, S cerevisiae; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione; D003931:Diacetyl