Monolithic silica capillary column with coated cellulose tris(3,5-dimethylphenylcarbamate) for capillary electrochromatographic separation of enantiomers.
Monolithic silica capillary columns were prepared by a sol-gel process in fused-silica capillaries with an inner diameter of 50 microm and were modified by coating of cellulose tris(3,5-dimethylphenylcarbamate). Influences of the factors in the modification process on enantiomer separations were investigated. The prepared columns were used to perform enantiomer separations by CEC. Fifteen and two pairs of enantiomers were separated under aqueous and nonaqueous mobile phases, respectively, and most of them were baseline-separated with very high column efficiencies. The Van Deemter curve was found flat under high linear velocity of the mobile phase, which indicated favorable kinetic properties of the prepared columns. Baseline separation of a pair of enantiomers was achieved in 90 s with high-column efficiency by short-end separation under high voltage.
Qin
Feng
F
National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
Xie
Chuanhui
C
Feng
Shun
S
Ou
Junjie
J
Kong
Liang
L
Ye
Mingliang
M
Zou
Hanfa
H
D002482:Cellulose / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D048448:Phenylcarbamates; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D013237:Stereoisomerism; D014867:Water
D048448:Phenylcarbamates; D014867:Water; C065943:cellulose tris-3,5-dimethylphenyl-carbamate; D012822:Silicon Dioxide; D002482:Cellulose