Requirement of norD for Brucella suis virulence in a murine model of in vitro and in vivo infection.
A mutant of Brucella suis bearing a Tn5 insertion in norD, the last gene of the operon norEFCBQD, encoding nitric oxide reductase, was unable to survive under anaerobic denitrifying conditions. The norD strain exhibited attenuated multiplication within nitric oxide-producing murine macrophages and rapid elimination in mice, hence demonstrating that norD is essential for Brucella virulence.
INSERM U431, Université Montpellier II, Place E. Bataillon, CC100, 34095 Montpellier Cedex 05, France.
Séverine
S
Loisel-Meyer
Maria Pilar
MP
Jiménez de Bagüés
Eugénie
E
Bassères
Jacques
J
Dornand
Stephan
S
Köhler
Jean-Pierre
JP
Liautard
Véronique
V
Jubier-Maurin
D000818:Animals; D041021:Brucella suis / Q000235:genetics / Q000472:pathogenicity*; D002006:Brucellosis / Q000382:microbiology*; D004195:Disease Models, Animal; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D010088:Oxidoreductases / Q000235:genetics / Q000502:physiology*; D014774:Virulence / Q000502:physiology*
D009569:Nitric Oxide; D010088:Oxidoreductases; C034747:nitric-oxide reductase