[Determination of C21 steroidal glycosides in Marsdenia tenacissima by colorimetric method].
OBJECTIVE
To establish a quantitative method to deter mine C21 steroidal glycosides in Marsdenia tenacissima.


METHODS
Methanol was used as the extraction solvent and the samples were purified by macroporous resin ADS-7. A mixture of sulfuric acid-methanol (4:1)was used as color-producing reagent. The absorbance was measured at 325 nm.


RESULTS
There is a good linearity (r = 0.999 9, n = 6) within the range of 10.6-148.4 microg. The average recoveries of tenacissoside-H at different concentrations were 95.8% to 97.1% with RSD less than 2.4% (n = 5).


CONCLUSIONS
This method is reliable as a quantitative analytical method for the quality assessment of M. tenacissima.
Shen
Fan
F
School of Pharmacy, Fudan University, Shanghai 200032, China.
Chen
Dao-feng
DF
D003124:Colorimetry / Q000379:methods*; D006027:Glycosides / Q000032:analysis*; D031101:Marsdenia / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders; D011786:Quality Control; D015203:Reproducibility of Results; D013256:Steroids / Q000032:analysis*
D006027:Glycosides; D011208:Powders; D013256:Steroids