Radiation processing to ensure safety of minimally processed carrot (Daucus carota) and cucumber (Cucumis sativus): optimization of dose for the elimination of Salmonella Typhimurium and Listeria monocytogenes.
Minimally processed vegetables are in demand, because they offer convenience to consumers. However, these products are often unsafe because of possible contamination with pathogens, such as Salmonella, Escherichia coli O157:H7, and Shigella species. Therefore, this study was carried out to optimize the radiation dose necessary to ensure the safety of precut carrot and cucumber. Decimal reduction doses (D-values) of Salmonella Typhimurium MTCC 98 were ca. 0.164 kGy in carrot samples and 0.178 kGy in cucumber samples. D-values of Listeria monocytogenes were determined to be 0.312 and 0.345 kGy in carrot and cucumber samples, respectively. Studies of inoculated, packaged, minimally processed carrot and cucumber samples showed that treatment with a 1-kGy dose of gamma radiation eliminated up to 4 log CFU/g of Salmonella Typhimurium and 3 log CFU/g of L. monocytogenes. However, treatment with a 2-kGy dose was necessary to eliminate these pathogens by 5 log CFU/g. Storage studies showed that both Salmonella Typhimurium and L. monocytogenes were able to grow at 10 degrees C in inoculated control samples. Neither of these pathogens could be recovered from radiation-processed samples after storage for up to 8 days.
Food Technology Division, Bhabha Atomic Research Centre, Mumbai, India.
V S
VS
Dhokane
S
S
Hajare
R
R
Shashidhar
A
A
Sharma
J R
JR
Bandekar
D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D018553:Cucumis sativus / Q000382:microbiology*; D018552:Daucus carota / Q000382:microbiology*; D004307:Dose-Response Relationship, Radiation; D005514:Food Irradiation / Q000379:methods*; D005519:Food Preservation / Q000379:methods; D005720:Gamma Rays; D006801:Humans; D008089:Listeria monocytogenes / Q000254:growth & development / Q000528:radiation effects*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000528:radiation effects*