Microfluorometry of cell membrane dynamics.
Membranes of living cells are characterized by a combination of conventional and total internal reflection fluorescence microscopy (TIRFM) using the membrane marker laurdan. In the first case, all cellular membranes are assessed simultaneously, whereas in the second case, the plasma membrane is excited selectively by the evanescent electromagnetic field of a laser beam. A spectral shift depending on the phase of membrane lipids is used to characterize membrane stiffness, which decreases with temperature and increases with the amount of cholesterol. Spectral properties are evaluated and displayed as microscopic images.
Hochschule Aalen, Institut für Angewandte Forschung, 73430 Aalen, Germany.
Petra
P
Weber
Michael
M
Wagner
Herbert
H
Schneckenburger
D015081:2-Naphthylamine / Q000031:analogs & derivatives / Q000737:chemistry; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects; D002784:Cholesterol / Q000737:chemistry / Q000494:pharmacology; D003592:Cytophotometry / Q000379:methods*; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007425:Intracellular Membranes / Q000737:chemistry / Q000187:drug effects; D007848:Laurates / Q000737:chemistry; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000494:pharmacology
D005456:Fluorescent Dyes; D007848:Laurates; D008563:Membrane Lipids; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D002784:Cholesterol; D015081:2-Naphthylamine; C065580:laurdan