Development and mathematical modeling of a two-stage reactor system for trichloroethylene degradation using Methylosinus trichosporium OB3b.
A two-stage reactor system was developed for the continuous degradation of gas-phase trichloroethylene (TCE). Methylosinus trichosporium OB3b was immobilized on activated carbon in a TCE degradation reactor, trickling biofilter (TBF). The TBF was coupled with a continuous stirred tank reactor (CSTR) to allow recirculation of microbial cells from/to the TBF for the reactivation of inactivated cells during TCE degradation. The mass transfer aspect of the TBF was analyzed, and mass transfer coefficient of 3.9 h(-1) was estimated. The loss of soluble methane monooxygenase (sMMO) activity was modeled based on a material balance on the CSTR and TBF, and transformation capacity (T (c)) was determined to be 20.2 micromol mg(-1). Maximum TCE degradation rate of 525 mg 1(-1) d(-1) was obtained and reactor has been stably operated for more than 270 days.
School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea.
Jae Woong
JW
Hwang
Young Bum
YB
Choi
Sunghoon
S
Park
Cha Yong
CY
Choi
Eun Yeol
EY
Lee
D019149:Bioreactors / Q000382:microbiology*; D004785:Environmental Pollutants / Q000378:metabolism*; D004867:Equipment Design; D007700:Kinetics; D008433:Mathematics; D020602:Methylosinus trichosporium / Q000378:metabolism*; D008954:Models, Biological; D010105:Oxygenases / Q000378:metabolism; D013696:Temperature; D014241:Trichloroethylene / Q000378:metabolism*
D004785:Environmental Pollutants; D014241:Trichloroethylene; D010105:Oxygenases; C021555:methane monooxygenase