Use of highly sensitive mitochondrial probes to detect microchimerism in xenotransplantation models.
Chimerism, defined as the co-existence of cells of different origin within the same organism, has received much attention in hematopoietic cell and organ transplantation because of the strict relationship between its establishment and the induction of specific tolerance. Traditional methods applied for chimerism detection, such as immunohistochemistry, cytogenetics, fluorescent-activated cell sorter analysis, and serological and biochemical testing, are limited by their sensitivity. We have established a highly sensitive molecular approach based on the amplification of the mitochondrial cytochrome B gene and tested its specificity and sensitivity level in six different mammalian species, including human, pig, mouse, rat, sheep and rabbit. Increased sensitivity of detection of specific amplification products was obtained by the non-radioactive Southern blot technique. This novel approach allows the detection of one cell against the background of 1 to 4 x 10(6) xenogenec cells and will be helpful for high-sensitivity analysis of donor cell engraftment after xenotransplantation procedures in these animal models.
Soncini
Maddalena
M
Centro di Ricerca E. Menni, Fondazione Poliambulanza Istituto Ospedaliero, Brescia, Italy.
Signoroni
Patrizia Bonassi
PB
Bailo
Marco
M
Zatti
Daniela
D
Gregori
Alessandra
A
Lombardi
Guerino
G
Albertini
Alberto
A
Wengler
Georg S
GS
Parolini
Ornella
O
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D046528:Chimerism; D045303:Cytochromes b / Q000235:genetics / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000032:analysis; D006801:Humans; D008928:Mitochondria / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014183:Transplantation, Heterologous
D015415:Biomarkers; D004272:DNA, Mitochondrial; D045303:Cytochromes b