[Study in the mechanism of Shuxuetong injection on antithrombosis and thrombolysis].
OBJECTIVE
To study the effect of Shuxuetong injection on cerebral tissue and brain microvascular endothelial cell(BMEC) in secreting tissue-type plasminogen activator (tPA).


METHODS
The experiment in vivo was designed for three groups: sham-operation group, focal cerebral ischemia model group, Shuxuetong injection-treated group. The gene expression and the activity of tPA on brains were detected by RT-PCR and chromogenic substrate analysis respectively after treatment for five days; The experiment in vitro: The cultured BMEC were divided into experimental group and control group. The Shuxuetong injection were added into experimental group and the BMECs in the experimatal groups were treated for 24 h. The gene expression and the activity of tPA on cultured BMEC were detected by RT-PCR and chromogenic substrate analysis respectively.


RESULTS
The gene expression and the content of tPA on both cerebral tissue and BMEC in the experimatal groups were significantly higher than that in the control groups.


CONCLUSIONS
Shuxuetong injection could accelerate secretion of tPA, and therefore enhance fibrinolysis.
Zhang
Xuan
X
The Second Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400010, China. sdjnzx881@tom.com
Xiao
Bing
B
Hu
Chang-Lin
CL
D000818:Animals; D001921:Brain / Q000098:blood supply*; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D004338:Drug Combinations; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005343:Fibrinolytic Agents / Q000494:pharmacology; D007267:Injections; D007865:Leeches / Q000737:chemistry; D008297:Male; D008419:Materia Medica / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D008833:Microcirculation / Q000166:cytology; D009835:Oligochaeta / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics
D004338:Drug Combinations; D005343:Fibrinolytic Agents; D008419:Materia Medica; D012333:RNA, Messenger; D010959:Tissue Plasminogen Activator