Laser scanning cytometry in human brain slices.
BACKGROUND
The Laser Scanning Cytometry (LSC) offers quantitative fluorescence analysis of cell suspensions and tissue sections.


METHODS
We adapted this technique to immunohistochemical labelled human brain slices.


RESULTS
We were able to identify neurons according to their labelling and to display morphological structures such as the lamination of the entorhinal cortex. Further, we were able to distinguish between neurons with and without cyclin B1 expression and we could assign the expression of cyclin B1 to the cell islands of layer II and the pyramidal neurons of layer V of the entorhinal cortex in Alzheimer's disease effected brain. In addition, we developed a method depicting the three-dimensional distribution of the cells in intact tissue sections.


CONCLUSIONS
In this pilot experiments we could demonstrate the power of the LSC for the analysis of human brain sections.
Mosch
Birgit
B
Department of Neuroanatomy, Paul Flechsig Institute of Brain Research, University of Leipzig, Leipzig, Germany. mosch@medizin.uni-leipzig.de
Mittag
Anja
A
Lenz
Dominik
D
Arendt
Thomas
T
Tárnok
Attila
A
D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D001923:Brain Chemistry; D019926:Cyclin B / Q000032:analysis; D056744:Cyclin B1; D018728:Entorhinal Cortex / Q000737:chemistry / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D047569:Laser Scanning Cytometry / Q000379:methods*; D016900:Neurofilament Proteins / Q000032:analysis; D009457:Neuroglia / Q000737:chemistry / Q000473:pathology; D009474:Neurons / Q000737:chemistry / Q000473:pathology; D020534:Parahippocampal Gyrus / Q000737:chemistry / Q000473:pathology; D010865:Pilot Projects; D011419:Propidium / Q000737:chemistry
C534699:CCNB1 protein, human; D019926:Cyclin B; D056744:Cyclin B1; D016900:Neurofilament Proteins; D011419:Propidium