[Effects of dipfluzine on expressions of E-selectin, P-selectin, and ICAM-1 in brain ischemia-reperfusion rats].
OBJECTIVE
To evaluate the effects of dipfluzine on the expressions of E-selectin, P-selectin, and ICAM-1 and the infiltration of polymorphonuclear leukocytes in brain ischemia-reperfusion rats.


METHODS
The model of focal cerebral ischemia was established with the Zea-Longa occluding suture. Dipfluzine (0.25, 0.5 and 1 mg x kg(-1)), flunarizine 0.5 mg x kg(-1) and solvent were injected separately into lingual vein at 30 min after ischemia. The occluding suture was slowly taken away to cause reperfusion at 1 h after ischemia. Rats were decapitated under anesthesia at 24 h after ischemia-reperfusion and brains were immediately removed to do the following procedures. Effects of dipfluzine on morphology of the brain tissue were observed through hematoxylin-eosin (HE) staining. By immunohistochemistry and flow cytometry technique and biochemical method, effects of dipfluzine on P-selectin, E-selectin, ICAM-1 and myeloperoxidase (MPO) were observed.


RESULTS
Dipfluzine could relieve pathological damages in the brain tissue after ischemia-reperfusion, and reduce the expressions of E-selectin, P-selectin and ICAM-1 and activities of MPO in dose-dependent manner.


CONCLUSIONS
Dipfluzine depresses the expressions of P-selectin, E-selectin, and ICAM-1, which are correlated with their effects on the activities of MPO, suggesting that dipfluzine has anti-inflammation effect in certain extent and could protect brain tissue from ischemia-reperfusion injury.
Department of Pharmacology, Hebei Medical University, Shijiazhuang 050017, China.
Guo-hong
GH
Zhang
Ping
P
Lü
Yong-li
YL
Wang
D000818:Animals; D002545:Brain Ischemia / Q000209:etiology; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D002936:Cinnarizine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019040:E-Selectin / Q000378:metabolism*; D020244:Infarction, Middle Cerebral Artery / Q000150:complications; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology; D019007:P-Selectin / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism* / Q000473:pathology
D019040:E-Selectin; D018696:Neuroprotective Agents; D019007:P-Selectin; D018799:Intercellular Adhesion Molecule-1; D002936:Cinnarizine; C065758:dipfluzine; D009195:Peroxidase