In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts.
OBJECTIVE
The aim of this study was to assess the cytotoxicity of 4 resin composites' eluates on WEHI 13 Var fibroblasts as they aged in a biologic medium.


METHODS
Cytotoxicity was determined by counting the number of viable cells by trypan blue exclusion. Morphologic changes attributable to cytotoxicity were observed by May-Grunwald-Giemsa cytologic staining and microscopic examination. DNA gel electrophoresis was performed to detect possible genotoxicity and DNA damage.


RESULTS
All resin composite eluates, except that for Targis, caused a pronounced cytotoxicity during the first 72 hours that gradually decreased after 2 weeks of aging. Severe morphologic alterations and pronounced DNA damage were also observed.


CONCLUSIONS
These findings indicate that resin-based dental restorative materials release agents cytotoxic and genotoxic to fibroblasts. Cytotoxicity is gradually decreased as the composite resins age in a biologic-relevant medium.
Bakopoulou
Athina A
AA
Department of Fixed & Implant Prosthodontics, School of Dentistry, Aristotle University of Thessaloniki, Greece. athinabakopoulou@hotmail.com
Triviai
Ioanna N
IN
Tsiftsoglou
Asterios S
AS
Garefis
Paylos D
PD
D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000633:toxicity; D004396:Coloring Agents; D003188:Composite Resins / Q000633:toxicity*; D004249:DNA Damage; D003764:Dental Materials / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D005899:Glass Ionomer Cements / Q000633:toxicity; D008422:Materials Testing; D009153:Mutagens / Q000633:toxicity; D012823:Silicate Cement / Q000633:toxicity; D013997:Time Factors; D014343:Trypan Blue
D004396:Coloring Agents; D003188:Composite Resins; D003764:Dental Materials; C444701:Filtek Z250; D005899:Glass Ionomer Cements; D009153:Mutagens; C510232:Simile composite resin; C121818:Tetric ceram; C111841:ceromer; C111282:dental polyglass; D012823:Silicate Cement; D014343:Trypan Blue