Isolation and characterization of a novel thraustochytrid-like microorganism that efficiently produces docosahexaenoic acid.
A thraustochytrid-like microorganism (strain 12B) was isolated from the mangrove area of Okinawa, Japan. On the basis of its ectoplasmic net structure and biflagellate zoospores we determined strain 12B to be a novel member of the phylum Labyrinthulomycota in the kingdom Protoctista. When grown on glucose/seawater at 28 degrees C, it had a lipid content of 58% with docosahexaenoic acid (DHA; 22:6 n-3) at 43% of the total fatty acids. It had a growth rate of 0.38 h(-1). The DHA production rate of 2.8 +/- 0.7 g l(-1) day(-1) is the highest value reported for any microorganism.
Perveen
Zakia
Z
Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Kita-ku, 060-0810, Japan.
Ando
Hitomi
H
Ueno
Akio
A
Ito
Yukiya
Y
Yamamoto
Yusuke
Y
Yamada
Yohko
Y
Takagi
Tomoko
T
Kaneko
Takako
T
Kogame
Kazuhiro
K
Okuyama
Hidetoshi
H
D004281:Docosahexaenoic Acids / Q000378:metabolism*; D005658:Fungi / Q000378:metabolism*; D050356:Lipid Metabolism; D019684:Magnoliopsida / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology*
D004281:Docosahexaenoic Acids