Macrophages in human visceral adipose tissue: increased accumulation in obesity and a source of resistin and visfatin.
OBJECTIVE
Increased visceral white adipose tissue (WAT) is linked to the risk of developing diabetes.


RESULTS
We showed by fluorescence activated cell sorting analysis that human visceral WAT contains macrophages, the proportion of which increased with obesity. Selective isolation of mature adipocytes and macrophages from human visceral WAT by CD14 immunoselection revealed that macrophages expressed higher levels of chemokines (monocyte chemotactic protein 1, macrophage inflammatory protein 1alpha, IL-8) and the adipokines resistin and visfatin than did mature adipocytes, as assessed by real-time PCR analysis. Moreover, resistin and visfatin proteins were found to be released predominantly by visceral WAT macrophages. Macrophage-derived secretory products stimulated phosphorylation of protein kinase B in human hepatocytes.


CONCLUSIONS
Resistin and visfatin might be considered to be proinflammatory markers. The increased macrophage population in obese human visceral WAT might be responsible for the enhanced production of chemokines as well as resistin and visfatin.
Curat
C A
CA
Institute of Cardiovascular Physiology, Johann Wolfgang Goethe University, Theodor-Stern Kai 7, 60590, Frankfurt am Main, Germany.
Wegner
V
V
Sengenès
C
C
Miranville
A
A
Tonus
C
C
Busse
R
R
Bouloumié
A
A
D050153:Abdominal Fat / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002465:Cell Movement; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D054409:Nicotinamide Phosphoribosyltransferase; D009765:Obesity / Q000378:metabolism*; D052243:Resistin / Q000378:metabolism*
D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D052243:Resistin; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human