Development of capillary column packed with thiol-modified gold-coated polystyrene particles and its selectivity for aromatic compounds.
Three types of thiol compounds (n-octadecanethiol, thiophenol, and 2-phenylethanethiol) were used to modify the gold-coated polystyrene particles (dp. 5microm) to prepare a stationary phase for capillary liquid chromatography through the formation of self-assembled monolayer. The column with n-octadecanethiol-modified gold-coated polystyrene particles (C18-Au) demonstrated the higher affinity to phenanthrene and anthracene than small aromatics compared to the ODS column. In addition, the shape selectivity between phenanthrene and anthracene in the C18-Au column was much higher than that in the ODS column (separation factors: 1.82 and 1.14, respectively). The relationship between the retention factor and acetonitrile content in the mobile phase revealed that the retention behaviors in the C18-Au column was more sensitive on the acetonitrile content than those in the ODS column. Relatively higher affinity for phenanthrene and anthracene was commonly observed in all the three thiol-modified Au columns than that for the conventional ODS column, whereas separations of benzene and nitro- and chlorobenzenes were quite different among the three thiol-modified Au and ODS columns.
Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso, Showa, Nagoya 466-8555, Japan.
Ken
K
Kobayashi
Shinya
S
Kitagawa
Hajime
H
Ohtani
D000097:Acetonitriles / Q000302:isolation & purification; D000873:Anthracenes / Q000302:isolation & purification; D001554:Benzene / Q000302:isolation & purification; D002722:Chlorobenzenes / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006841:Hydrocarbons, Aromatic / Q000302:isolation & purification*; D009578:Nitrobenzenes / Q000302:isolation & purification; D010316:Particle Size; D010616:Phenanthrenes / Q000302:isolation & purification; D011137:Polystyrenes / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013997:Time Factors
D000097:Acetonitriles; D000873:Anthracenes; D002722:Chlorobenzenes; D006841:Hydrocarbons, Aromatic; D009578:Nitrobenzenes; D010616:Phenanthrenes; D011137:Polystyrenes; D013438:Sulfhydryl Compounds; C055360:n-octadecyl mercaptan; C031181:phenanthrene; D006046:Gold; C034020:anthracene; D001554:Benzene; C032159:acetonitrile