Expression of a novel gene, MafB, in Dupuytren's disease.
OBJECTIVE
Dupuytren's disease (DD) is characterized by fibroblastic proliferation of the palmar fascia, often leading to flexion contracture in the hand. Although there is a strong genetic component the genome-wide expression of novel genes is not known. The purpose of this study was to use DNA microarray technology to identify upregulated genes in DD.


METHODS
Human tissue samples were harvested from 3 patient sources: DD cord tissue (n = 20), normal-appearing adjacent control fascia (n = 15), and palmar fascia from patients having carpal tunnel release (n = 15). DNA microarray analysis was performed on amplified sample RNA. Novel genes were compared with known gene functions. A candidate gene of interest was studied further by using immunohistochemistry on DD tissue samples and controls.


RESULTS
Several novel genes not described previously in the study of DD were upregulated significantly, including MafB, collagen type V, alpha-2 (COL5A2), collagen type VIII, alpha-1 (COL8A1), contactin I (CNTN1), and leucine-rich repeat containing 17 (LRRC17). These upregulated genes were compared with their known gene-expression profiles in other tissues and their purported functions. MafB was found to be of particular interest because of its prominent role in tissue development and cellular differentiation. MafB immunohistochemistry showed positive staining in 50% of the DD specimens but complete absence of MafB in all control tissues (adjacent control fascia, carpal tunnel fascia). Co-localization experiments with MafB and alpha-smooth muscle actin showed staining properties in similar regions but these 2 proteins were not confined solely to the same cells.


CONCLUSIONS
Microarray analysis of DD tissue has identified significant upregulated gene expression of MafB. MafB protein also is found in Dupuytren's cords but not in control fascia. Co-localization data suggest that the association of MafB with DD is not related exclusively to myofibroblast proliferation. Because of its role in fibroblastic transformation in other models MafB and its relationship to the pathogenesis of DD deserves further study.
Lee
Lucy C
LC
Stanford University School of Medicine, Stanford, CA, USA.
Zhang
Andrew Y
AY
Chong
Alphonsus K
AK
Pham
Hung
H
Longaker
Michael T
MT
Chang
James
J
D000199:Actins / Q000378:metabolism; D000328:Adult; D000368:Aged; D004387:Dupuytren Contracture / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D005205:Fascia / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D051258:MafB Transcription Factor / Q000378:metabolism*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation
D000199:Actins; C490407:MAFB protein, human; D051258:MafB Transcription Factor