[Research of a new kind of biological response modifier to anti-laryngocarcinoma].
OBJECTIVE
To find a new kind of biological response modifier to anti-laryngocarcinoma through studying the efficiency of resveratrol.


METHODS
To detect the growth inhibiting rate of Hep-2 exposed to resveratrol with MTT colorimetry and to analyze the apoptosis and cell cycle of Hep-2 with flow cytometry. As having formed the model of laryngocarcinoma, quite a few indicatrixes including the content changes of IL-2, IL-8,TGF-beta1 and VEGF were detected.


RESULTS
Resveratrol could induce apoptosis of Hep-2 in time-concentration-dependent manners showing obvious cell cycle blocking of G0/G1 and apoptotic peak. Furthermore, resveratrol improved remarkably the growth condition of mice planted with Hep-2 while enhancing their immunological function.


CONCLUSIONS
As a kind of biological response modifier, resveratrol could strengthen anti-tumor immunoresponse, accommodating lymphocyte to secrete cytokine, through which it exerted the efficiency of anti-laryngocarcinoma.
Department of Immunology, Medical School of Xi'an Jiaotong University, Xi'an, 710061, China.
Tan
T
Li
Guixiang
G
Fan
Tong
T
Li
Yukang
Y
Yuan
Lin
L
Shi
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D007376:Interleukin-2; D016209:Interleukin-8; D013267:Stilbenes; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D000077185:Resveratrol