Genotyping of Chlamydia trachomatis from clinical specimens in Taiwan.
This study was conducted to determine the prevalence and distribution of Chlamydia trachomatis genotypes in Taiwan. Urine and endocervical-swab samples were collected from two hospitals located in northern and southern Taiwan. The genotypes of a total of 145 samples positive for C. trachomatis were analysed by sequencing the omp1 gene and this was successful in 102 samples. Nine different C. trachomatis genotypes were identified. Genotype E was the most prevalent (22 %), followed by D and Da (19 %), F (16 %), J (15 %), K (11 %), G (11 %), H (6 %) and Ba (2 %). There was a geographical difference in the prevalence of genotype H (P < 0.018) between northern and southern Taiwan. Sequence mutation analysis by blast searching against GenBank reference sequences identified 12 genetic variants from a total of 102 omp1 gene sequences.
Laboratory for Bacteriology and Mycology, Center for Laboratory Research and Diagnostics, Center for Disease Control, Taipei, Taiwan.
Min-Chih
MC
Hsu
Laboratory for Bacteriology and Mycology, Center for Laboratory Research and Diagnostics, Center for Disease Control, Taipei, Taiwan.
Pei-Yi
PY
Tsai
Department of Public Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Kow-Tong
KT
Chen
Taipei City Hospital, Branch for Disease Control & Prevention, Taipei, Taiwan.
Lan-Hui
LH
Li
Taipei City Hospital, Branch for Disease Control & Prevention, Taipei, Taiwan.
Chien-Chou
CC
Chiang
Chung-Ho Memorial Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
Jih-Jin
JJ
Tsai
Chung-Ho Memorial Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
Liang-Yin
LY
Ke
Laboratory for Bacteriology and Mycology, Center for Laboratory Research and Diagnostics, Center for Disease Control, Taipei, Taiwan.
Hour-Young
HY
Chen
Laboratory for Bacteriology and Mycology, Center for Laboratory Research and Diagnostics, Center for Disease Control, Taipei, Taiwan.
Shu-Ying
SY
Li
D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000453:epidemiology* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D052776:Female Urogenital Diseases / Q000453:epidemiology* / Q000382:microbiology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D018272:Porins / Q000235:genetics; D015995:Prevalence; D017422:Sequence Analysis, DNA; D013624:Taiwan / Q000453:epidemiology; D014556:Urine / Q000382:microbiology
D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis