Biosynthesis of medium-chain-length poly(hydroxyalkanoates) from soy molasses.
Pseudomonas corrugata was selected from a screening process for the bioconversion of inexpensive soy molasses into medium-chain-length poly(hydroxyalkanoates) (mcl-PHA). We obtained yields of 1.5 g cell dry weight (CDW)/l culture with growth medium supplemented with 2% (w/v) soy molasses, and of an average of 3.4 g CDW/l with 5% (w/v) soy molasses. Crude PHAs were obtained at 5-17% of CDW. The most prominent repeat-unit monomers in the PHAs were 3-hydroxydodecanoate, 3-hydroxyoctanoate, 3-hydroxydodecanoate, and 3-hydroxytetradecenoate. This work represents the first description of fermentative mcl-PHA production from the soy molasses.
Solaiman
Daniel K Y
DK
Fats, Oils and Animal Coproducts Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, Pennsylvania, 19038, USA. dsolaiman@errc.ars.usda.gov
Ashby
Richard D
RD
Hotchkiss
Arland T
AT
Foglia
Thomas A
TA
D002210:Caprylates / Q000378:metabolism*; D007850:Lauric Acids / Q000378:metabolism*; D008965:Molasses / Q000382:microbiology*; D009227:Myristic Acids / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry*
D002210:Caprylates; D007850:Lauric Acids; D009227:Myristic Acids; C022145:3-hydroxydodecanoic acid; C009893:beta-hydroxymyristic acid; C101940:3-hydroxyoctanoic acid