Determination of dopamine in rat striatum by microdialysis and high-performance liquid chromatography with electrochemical detection on a functionalized multi-wall carbon nanotube electrode.
High performance liquid chromatography coupled with microdialysis sampling and electrochemical detection (HPLC-ECD) has been used to determine dopamine (DA). In the HPLC-ECD a multi-wall carbon nanotube electrode chemically modified with carboxyl groups (MWNT-COOH CME) was used as the working electrode for determination of DA. The results indicated that the MWNT-COOH CME enabled efficient electrocatalytic oxidation of DA with relatively high sensitivity and stability and long life. Peak currents for dopamine were linearly dependent on concentration in the range 5.0 x 10(-9) to 5.0 x 10(-5) mol L(-1) and the calculated detection limit (S/N=3) was 2.5 x 10(-9) mol L(-1). The method had been successfully used to measure dopamine in rat striatal microdialysate. To study the physiological effect of nitric oxide (NO) on striatal release of DA, 0.5 mmol L(-1) sodium nitroprusside (SNP) was a continuously perfused into rat striatum. This resulted in a 46% increase in DA basal level.
Department of Pharmacy, Wenzhou Medical College, 325035, Wenzhou, People's Republic of China. linliwz@21cn.com
Li
L
Lin
Peihong
P
Qiu
Lizhu
L
Yang
Xuni
X
Cao
Litong
L
Jin
D000818:Animals; D015374:Biosensing Techniques; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D017551:Microdialysis; D036103:Nanotechnology; D037742:Nanotubes, Carbon; D009569:Nitric Oxide / Q000494:pharmacology; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
D037742:Nanotubes, Carbon; D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide; D004298:Dopamine