[The molecule mechanism of soybean isoflavaones antagonizing bone loss induced by postmenopausal osteoporosis].
OBJECTIVE
To observe the effect of the Soybean Isoflavaones on the mRNA expression of Osteoprotegerin (OPG), Osteoclast Differentiation Factor (ODF) and Macrophage Colony stimulating Factor (M-CSF) in bone tissue of ovariectomized rat, and to investigate the possible molecule mechanism of Soybean Isoflavaones antagonizing bone lose induced by postmenopausal osteoporosis.


METHODS
Thirty healthy adult SD rats were randomly divided into 3 groups: sham-operated group, ovariectomized group and Soybean Isoflavaones treated group. All rats were ovariectomized except those in sham-operated group. Bone density of the 3-6th lumbar vertebrae were detected after 12 weeks. Total RNA were extracted from femur bone and the mRNA expression of OPG, ODF and M-CSF were examined by real time PCR.


RESULTS
The data showed that Soybean Isoflavaones increased the bone density of the ovariectomized rat lumbar vertebrae and up-regulated the expression of OPG,whereas down-regulated the expression of M-CSF and the ratio of ODF:OPG.


CONCLUSIONS
The molecule mechanism of Soybean Isoflavaones antagonizing bone loss induced by postmenopausal osteoporosis is tightly correlated with the regulation of expression of OPG,M-CSF and the ratio of ODF:OPG.
Department of Orthopaedics Surgery, Second Hospital of Xi'an Jiaotong University, Xi'an 71004.
Qiang
Q
Wang
Kunzheng
K
Wang
Xiangpei
X
Wang
D000818:Animals; D015519:Bone Density; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D007529:Isoflavones / Q000494:pharmacology*; D008159:Lumbar Vertebrae / Q000187:drug effects / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism; D010024:Osteoporosis / Q000517:prevention & control*; D053244:Osteoprotegerin; D010052:Ovariectomy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis* / Q000235:genetics; D013025:Glycine max / Q000737:chemistry*
D002352:Carrier Proteins; D006023:Glycoproteins; D007529:Isoflavones; D008562:Membrane Glycoproteins; D053244:Osteoprotegerin; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506032:Tnfrsf11b protein, rat; D016173:Macrophage Colony-Stimulating Factor