Acetyl-CoA synthetase overexpression in Escherichia coli demonstrates more efficient acetate assimilation and lower acetate accumulation: a potential tool in metabolic engineering.
The overexpression of acetyl-CoA (CoA) synthetase (ACS) in Escherichia coli showed significant reduction in acetate during glucose fermentation. It also greatly enhanced acetate assimilation when acetate was used as a carbon source. These features are ideal for applications in metabolic engineering. ACS overexpression can be strategically applied to reduce acetate byproduct, recover wasted carbon, and redirect carbon flux toward more favorable pathways. The native acs gene was cloned and overexpressed in E. coli. Studies showed significant effects on acetate production and assimilation in cultures grown in minimal and complex media with glucose or acetate as the carbon source.
Lin
Henry
H
Department of Bioengineering, Rice University, Houston, TX, USA.
Castro
Natalia M
NM
Bennett
George N
GN
San
Ka-Yiu
KY
D000106:Acetate-CoA Ligase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D000085:Acetates / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D015202:Protein Engineering / Q000379:methods
D000085:Acetates; D001426:Bacterial Proteins; D000106:Acetate-CoA Ligase