[Determination of apomorphine, sildenafil and alprostadil in medicines for erectile dysfunction by high performance liquid chromatography-mass spectrometry].
A high performance liquid chromatography-mass spectrometry (LC-MS) analytical method for illicit drugs, apomorphine, sildenafil and alprostadil, in medicines for erectile dysfunction has been developed. The samples were extracted with methanol using ultrasound-assisted extraction. The chromatographic separation was performed on a Zorbax Eclipse XDB-C18 column using acetonitrile-0.5% formic acid aqueous solution as mobile phase. The three compounds were identified by retention time and m/z and quantified by peak area. The results demonstrated that the linear ranges were 50.0 - 5 000.0 microg/L, 10.0 - 1 000.0 microg/L, 40.0 - 4 000.0 microg/L, with detection limits of 20.0, 4.0, 10.0 microg/L for apomorphine, sildenafil and alprostadil, respectively. The average recoveries and the relative standard deviations were 89% - 95% and 9.5% - 11%. The method is simple, rapid, accurate and suitable for the simultaneous determination of these drugs in medicines for erectile dysfunction.
Engineering Center for Sugar Cane and Cane Sugar, Guangxi University, Nanning 530004, China. yjxu@gxu.edu.cn
Yuanjin
Y
Xu
Guiping
G
Xu
D000527:Alprostadil / Q000032:analysis* / Q000627:therapeutic use; D001058:Apomorphine / Q000032:analysis* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018491:Dopamine Agonists / Q000032:analysis / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000627:therapeutic use; D007172:Erectile Dysfunction / Q000188:drug therapy*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D010726:Phosphodiesterase Inhibitors / Q000032:analysis / Q000627:therapeutic use; D010879:Piperazines / Q000032:analysis* / Q000627:therapeutic use; D011687:Purines / Q000032:analysis / Q000627:therapeutic use; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000032:analysis* / Q000627:therapeutic use
D018491:Dopamine Agonists; D004365:Drugs, Chinese Herbal; D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D000068677:Sildenafil Citrate; D000527:Alprostadil; D001058:Apomorphine