[Changes in cyclooxygenase gene expression during spontaneous recovery from stress ulcer in rats].
OBJECTIVE
To observe the changes in gene expression of cyclooxygenase (COX) during spontaneous recovery from stress ulcer in rats exposed to water immersion and restraint stress (WRS).


METHODS
A rat model of stress ulcer was established by means of WRS, in which the changes in COX expression were detected with immunohistochemistry and reverse transcription (RT)-PCR.


RESULTS
Very low levels of COX-2 expression were detected in the gastric mucosa of the control rats, and the expression increased significantly during the healing process of the stress ulcer (P<0.05). COX-1 expression in the gastric mucosa showed no significant difference between the control group and the stress ulcer groups during healing (P>0.05).


CONCLUSIONS
COX-1 and COX-2 expressions in rat gastric mucosa during the recovery from stress ulcer participate in the recovery of the damaged mucosa possibly by mediating prostaglandin secretion.
Xu
Jun
J
Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. xzy1996@fimmu.com
Song
Yu-gang
YG
Sang
Xian-fu
XF
Bao
Guang-xin
GX
Li
Xu
X
Wu
Gang
G
Chen
Dong-shen
DS
D000818:Animals; D051545:Cyclooxygenase 1 / Q000096:biosynthesis / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D005753:Gastric Mucosa / Q000201:enzymology; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012075:Remission, Spontaneous; D013276:Stomach Ulcer / Q000201:enzymology*; D013312:Stress, Physiological / Q000150:complications
D012333:RNA, Messenger; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases