Expression profiles of micro RNA in proliferating and differentiating 32D murine myeloid cells.
32D cells are murine myeloid cells that grow indefinitely in Interleukin-3 (IL-3). In these cells, the type 1 insulin-like growth factor (IGF-I) and granulocytic-colony stimulating factor (G-CSF) induce differentiation to granulocytes. 32D cells do not express insulin receptor substrate-1 (IRS-1) or IRS-2, docking proteins of the IGF-I receptor. Ectopic expression of IRS-1 in these cells inhibits differentiation, the cells become IL-3 independent and IGF-1 dependent and can form tumors in mice. 32D and 32D-derived cells offer a good model in which to study the expression profiles of Micro Rna (miR) related to sustained proliferation or differentiation. We present here the data obtained with miR micro-arrays and identify the miR that are regulated by IGF-1 or G-CSF and are associated with either differentiation or indefinite cell proliferation of 32D murine myeloid cells.
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Bin
B
Shi
Marco
M
Prisco
George
G
Calin
Chang-Gong
CG
Liu
Giuseppe
G
Russo
Antonio
A
Giordano
Renato
R
Baserga
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D055504:Insulin Receptor Substrate Proteins; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D051379:Mice; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D022423:Myeloid Cells / Q000166:cytology* / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000235:genetics
D055504:Insulin Receptor Substrate Proteins; C526295:Irs1 protein, mouse; D035683:MicroRNAs; D010750:Phosphoproteins; D016179:Granulocyte Colony-Stimulating Factor; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1