Immunolocalization of FsPK1 correlates this abscisic acid-induced protein kinase with germination arrest in Fagus sylvatica L. seeds.
An enzymatically active recombinant protein kinase, previously isolated and characterized in Fagus sylvatica L. dormant seeds (FsPK1), was used to obtain a specific polyclonal antibody against this protein. Immunoblotting and immunohistochemical analysis of FsPK1 protein in beech seeds showed a strong immunostaining in the nucleus of the cells located in the vascular tissue of the embryonic axis corresponding to the future apical meristem of the root. This protein kinase was found to accumulate in the seeds only when embryo growth was arrested by application of ABA, while the protein amount decreased during stratification, previously proved to alleviate dormancy, and no protein was detected at all when seed germination was induced by addition of GA(3). These results indicate that FsPK1 may be involved in the control of the embryo growth mediated by ABA and GAs during the transition from dormancy to germination in Fagus sylvatica seeds.
Reyes
David
D
Departamento de Fisiología Vegetal, Centro Hispano-Luso de Investigaciones Agrarias, Facultad de Biología, Universidad de Salamanca, Plaza de los Doctores de la Reina s/n, E-37007 Salamanca, Spain.
Rodríguez
Dolores
D
Lorenzo
Oscar
O
Nicolás
Gregorio
G
Cañas
Rafael
R
Cantón
Francisco R
FR
Canovas
Francisco M
FM
Nicolás
Carlos
C
D000040:Abscisic Acid / Q000494:pharmacology*; D000906:Antibodies / Q000276:immunology; D003080:Cold Temperature; D018548:Cotyledon / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development; D029964:Fagus / Q000187:drug effects / Q000196:embryology* / Q000201:enzymology*; D018525:Germination / Q000502:physiology*; D005875:Gibberellins / Q000494:pharmacology; D007150:Immunohistochemistry; D010940:Plant Proteins / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D011494:Protein Kinases / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D012639:Seeds / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
D000906:Antibodies; D005875:Gibberellins; D010940:Plant Proteins; D000040:Abscisic Acid; C007842:gibberellic acid; D011494:Protein Kinases