Redox regulation of mast cell histamine release in thioredoxin-1 (TRX) transgenic mice.
Thioredoxin-1 (TRX) is a stress-inducible redox-regulatory protein with antioxidative and anti-inflammatory effects. Here we show that the release of histamine from mast cells elicited by cross-linking of high-affinity receptor for IgE (FcepsilonRI) was significantly suppressed in TRX transgenic (TRX-tg) mice compared to wild type (WT) mice. Intracellular reactive oxygen species (ROS) of mast cells stimulated by IgE and antigen was also reduced in TRX-tg mice compared to WT mice. Whereas there was no difference in the production of cytokines (IL-6 and TNF-alpha) from mast cells in response to 2,4-dinitrophenylated bovine serum albumin (DNP-BSA) stimulation in TRX-tg and WT mice. Immunological status of TRX-tg mice inclined to T helper (Th) 2 dominant in primary immune response, although there was no difference in the population of dendritic cells (DCs) and regulatory T cells. We conclude that the histamine release from mast cells in TRX-tg mice is suppressed by inhibition of ROS generation. As ROS are involved in mast cell activation and facilitate mediator release, TRX may be a key signaling molecule regulating the early events in the IgE signaling in mast cells and the allergic inflammation.
Son
Aoi
A
1Department of Biological Responses, Institute for Virus Research, Kyoto University, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
Nakamura
Hajime
H
Kondo
Norihiko
N
Matsuo
Yoshiyuki
Y
Liu
Wenrui
W
Oka
Shin-ichi
S
Ishii
Yasuyuki
Y
Yodoi
Junji
J
D000818:Animals; D002417:Cattle; D003713:Dendritic Cells / Q000378:metabolism; D004140:Dinitrophenols / Q000276:immunology; D006241:Haptens / Q000276:immunology; D006636:Histamine Release; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010084:Oxidation-Reduction; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004140:Dinitrophenols; D006241:Haptens; D015850:Interleukin-6; D020033:Protein Isoforms; D017382:Reactive Oxygen Species; D017455:Receptors, IgE; D014409:Tumor Necrosis Factor-alpha; C515559:Txn1 protein, mouse; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D013879:Thioredoxins