Quantification of the efficiency of cargo delivery by peptidic and pseudo-peptidic Trojan carriers using MALDI-TOF mass spectrometry.
We have measured the efficiencies of two novel pseudo-peptidic carriers and various cell-penetrating peptides (Penetratin, (Arg)9 and the third helix of the homeodomain of Knotted-1) to deliver the same cargo inside cells. The cargo that was studied corresponds to the pseudo-substrate of protein kinase C. Cargo delivery was quantified using a recent method based on isotope labeling and MALDI-TOF MS. Results of cargo delivery were compared to the amounts of free CPP internalized inside cells. The third helix of Knotted gave the best results concerning free CPP cellular uptake. It was also found to be the most efficient carrier. This peptide thus emerges as a new CPP with very promising properties.
Université Pierre et Marie Curie-Paris6, boite 45, 4 place Jussieu, 75252 Paris cedex 05, France.
Baptiste
B
Aussedat
Sandrine
S
Sagan
Gérard
G
Chassaing
Gérard
G
Bolbach
Fabienne
F
Burlina
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism; D057846:Cell-Penetrating Peptides; D002478:Cells, Cultured; D004337:Drug Carriers / Q000032:analysis* / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000032:analysis* / Q000138:chemical synthesis / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides; D004337:Drug Carriers; D009842:Oligopeptides; C512367:P1 pseudo-peptidic carrier; C512368:P2 pseudo-peptidic carrier; C414409:penetratin