[Alternations of ADF and G-actin in renal tubular epithelial cell of neonatal rats during kidney ischemic Injury].
OBJECTIVE
To study the changes of ADF and G-actin in renal tubular epithelial cell of neonatal rats during kindney ischemic injury. To explore the interactions between ADF and G-actin and their relationships with actin cytoskeleton.


METHODS
Twenty-four neonatal rats were used to the experiments due to have the weights from 30 to 40 g respectively. Renal ischemia was induced by clamping the left renal pedicle for different periods of time that was in 10 min or 30 min. The kidneys of normal neonatal rats were functioned as the control group. Two kinds of primary antibodies, which one was rabbit anti-chick ADF antiserum and another was mouse monoclonal G-actin antibody, were used on one same section of kidney tissue. FITC-labeled and TRITC-labeled secondary antibodies were used to identify ADF and G-actin respectively. These samples were examined by using a fluorescene microscope.


RESULTS
In control group, ADF and G-actin showed to be diffused in cytoplasm of renal tubular epithelial cell. In ischemic injury group, the distributions of ADF and G-actin were changed significantly to apical region of tubular epithelial cells and the renal tubula lumen. Regardless of the ADF expression to not change but G-actin was increased after kidney ischemia.


CONCLUSIONS
Under the physiolgical condition, ADF and G-actin got a diffuse distribution in cytoplasm of renal tubular epitbelial cell. However, when the kidney got in ischemia, the distributions of ADF and G-actin were changed significantly to apica region of tubular epithelial cell or the renal tubular lumen.
Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Fan
F
Yang
Yu-jia
YJ
Yao
Ying
Y
Xiong
Li-xing
LX
Yuan
Bo
B
Yu
Yong-xiu
YX
Chen
D051339:Actin Depolymerizing Factors / Q000378:metabolism*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D004847:Epithelial Cells / Q000378:metabolism*; D007668:Kidney / Q000098:blood supply*; D007684:Kidney Tubules / Q000098:blood supply / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology*
D051339:Actin Depolymerizing Factors; D000199:Actins