Simple pulse-acquire NMR methods for the quantitative analysis of calcium, magnesium and sodium in human serum.
Simple pulse-acquire NMR methods are presented for accurate quantitation of calcium (Ca(2+)), magnesium (Mg(2+)) and sodium (Na(+)) in human serum. Ca(2+) and Mg(2+) can be determined simultaneously by (1)H NMR via their EDTA (ethylenediaminetetraacetic acid) complexes. Spectra are acquired before and after addition of EDTA, and the difference spectrum is used for integration of the signals from the complexes relative to an internal reference. Serum sodium can also be determined through pulse-acquire (23)Na NMR by integration of the free sodium signal relative to a reference signal (Na(+)+EDTA in a coaxial capillary tube). The method shows excellent accuracy and precision for all three metal ions. Slow chemical exchange between complexed and free EDTA at the natural pH of serum does not limit the accuracy of the determination of Ca(2+) and Mg(2+), and the large errors associated with spin-echo spectral editing methods are avoided.
Somashekar
B S
BS
Centre of Biomedical Magnetic Resonance, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow 226 014, India.
Ijare
Omkar B
OB
Nagana Gowda
G A
GA
Ramesh
V
V
Gupta
Sushil
S
Khetrapal
C L
CL
D002118:Calcium / Q000097:blood*; D004492:Edetic Acid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000097:blood*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D012016:Reference Values; D015203:Reproducibility of Results; D012964:Sodium / Q000097:blood*
D004492:Edetic Acid; D012964:Sodium; D008274:Magnesium; D002118:Calcium