Metallothionein gene from Tetrahymena thermophila with a copper-inducible-repressible promoter.
We describe a novel metallothionein gene from Tetrahymena thermophila that has a strong copper-inducible promoter. This promoter can be turned on and off rapidly, making it a useful system for induction of ectopic gene expression in Tetrahymena and enhancing its applications in cell and molecular biology, as well as biotechnology.
Boldrin
Francesco
F
Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35100 Padova, Italy. francesco.boldrin@unipd.it
Santovito
Gianfranco
G
Gaertig
Jacek
J
Wloga
Dorota
D
Cassidy-Hanley
Donna
D
Clark
Theodore G
TG
Piccinni
Ester
E
D000818:Animals; D003300:Copper / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008668:Metallothionein / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins; D016808:Tetrahymena thermophila / Q000166:cytology / Q000187:drug effects / Q000235:genetics*
D012333:RNA, Messenger; D011994:Recombinant Proteins; D003300:Copper; D008668:Metallothionein