Cloning and functional analysis of the sequences flanking mini-Tn5 in the magnetosomes deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1.
A magnetosome deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1 was generated by mini-Tn5 transposon mutagenesis, and a 5045-bp fragment flanking mini-Tn5 in NM4 was cloned by Anchored PCR. Sequencing analysis showed that this fragment involved six putative open reading frames (ORFs); the mini-Tn5 was inserted into ORF4. Functional complementary test indicated that the 5045-bp fragment was required for biosynthesis of magnetosomes in M. gryphiswaldense MSR-1. The protein encoded by ORF4 had 25% of identity with the chemotaxis protein CheYIII of Caulobacter crescentus CB15, and the protein encoded by ORF4 contained a conserved signal receiver domain that can receive the signal from the sensor partner of the bacterial two-component systems. It was suggested that the protein encoded by ORF4 may take part in the signal transduction relating to biosynthesis of magnetosomes.
State Key Laboratory for Agro-biotechnology and Department of Microbiology, China Agricultural University, Beijing 100094, China.
Feng
F
Li
Ying
Y
Li
Wei
W
Jiang
Zhenfang
Z
Wang
Jilun
J
Li
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000379:methods; D017353:Gene Deletion; D023061:Gene Order; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008280:Magnetics; D042201:Magnetospirillum / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D015388:Organelles / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D015398:Signal Transduction / Q000235:genetics
D001426:Bacterial Proteins; D004251:DNA Transposable Elements