FEZ1 dimerization and interaction with transcription regulatory proteins involves its coiled-coil region.
The fasciculation and elongation protein zeta1 (FEZ1) is a mammalian orthologue of the Caenorhabditis elegans protein UNC-76, which is necessary for axon growth in that nematode. In previous studies FEZ1 has been found to interact with protein kinase Czeta, DISC1, the agnoprotein of the human polyomavirus JC virus, and E4B, a U-box-type ubiquitin-protein isopeptide ligase. We reported previously that FEZ1 and its paralogue FEZ2 are proteins that interact with NEK1, a protein kinase involved in polycystic kidney disease and DNA repair mechanisms at the G(2)/M phase of the cell cycle. Here we report the identification of 16 proteins that interact with human FEZ1-(221-396) in a yeast two-hybrid assay of a human fetal brain cDNA library. The 13 interacting proteins of known functions take part either in transcription regulation and chromatin remodeling (6 proteins), the regulation of neuronal cell development (2 proteins) and cellular transport mechanisms (3 proteins) or participate in apoptosis (2 proteins). We were able to confirm eight of the observed interactions by in vitro pull-down assays with recombinant fusion proteins. The confirmed interacting proteins include FEZ1 itself and three transcription controlling proteins (SAP30L, DRAP1, and BAF60a). In mapping studies we found that the C-terminal regions of FEZ1, and especially its coiled-coil region, are involved in its dimerization, its heterodimerization with FEZ2, and in the interaction with 10 of the identified interacting proteins. Our results give further support to the previous speculation of the functional involvement of FEZ1 in neuronal development but suggest further that FEZ1 may also be involved in transcriptional control.
Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron, Rua Giuseppe Máximo Scolfaro 10.000, CP 6192, 13084-971 Campinas, SP, Brasil.
Eliana M
EM
Assmann
Marcos R
MR
Alborghetti
Maria E R
ME
Camargo
Jörg
J
Kobarg
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001369:Axons / Q000378:metabolism; D001665:Binding Sites; D001692:Biological Transport; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D017173:Caenorhabditis elegans; D015815:Cell Adhesion Molecules / Q000737:chemistry; D002455:Cell Division; D002845:Chromatography; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D016195:G2 Phase; D015723:Gene Library; D005978:Glutathione / Q000737:chemistry; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins / Q000737:chemistry*; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C528597:FEZ1 protein, human; C481525:FEZ2 protein, human; C118711:LZTS1 protein, human; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D025521:Tumor Suppressor Proteins; D005982:Glutathione Transferase; D005978:Glutathione