Transgenic mice.
For the past 20 years researchers have used transgenic mice to help understand the basic mechanisms associated with inherited human and animal diseases. The ability to integrate exogenous genetic information into the mouse genome has revolutionised the analysis of gene function. Both gene addition and gene replacement can be performed and the capability exists to create 'conditional' mutations and to study gene dosage effects. The aim of the present review is to provide a framework of information on transgenic mouse methodologies that can be applied to any area of research. A basic understanding of transgenic technology, recognising its advantages and disadvantages, is essential knowledge for the scientist in the 21st century.
Hickman-Davis
Judy M
JM
Department of Anesthesiology, 901 19th St. South, BMR2, Room 230, University of Alabama at Birmingham, Birmingham, AL 35205, USA. jmhd@uab.edu
Davis
Ian C
IC
D000818:Animals; D018014:Gene Transfer Techniques; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D034622:RNA Interference