Evaluation of bacterial and protozoal contamination of commercially available raw meat diets for dogs.
OBJECTIVE
To evaluate bacterial and protozoal contamination of commercially available raw meat diets for dogs.


METHODS
Prospective longitudinal study.


METHODS
240 samples from 20 raw meat diets for dogs (containing beef, lamb, chicken, or turkey), 24 samples from 2 dry dog foods, and 24 samples from 2 canned dog foods.


METHODS
Each product was purchased commercially on 4 dates approximately 2 months apart. Three samples from each product at each sampling period were evaluated via bacterial culture for non-type-specific Escherichia coli (NTSEC), Salmonella enterica, and Campylobacter spp. Antimicrobial susceptibility testing was performed on selected isolates. Polymerase chain reaction assays were used to detect DNA from Cryptosporidium spp, Neospora spp, and Toxoplasma spp in samples obtained in the third and fourth sampling periods.


RESULTS
One hundred fifty-three of 288 (53%) samples were contaminated with NTSEC. Both raw and prepared foods contained NTSEC during at least 1 culture period. Salmonella enterica was recovered from 17 (5.9%) samples, all of which were raw meat products. Campylobacter spp was not isolated from any samples. In 91 of 288 (31.6%) samples, there was no gram-negative bacterial growth before enrichment and in 48 of 288 (16.7%) samples, there was no aerobic bacterial growth before enrichment. Susceptibility phenotypes were variable. Cryptosporidium spp DNA was detected in 3 samples.


CONCLUSIONS
Bacterial contamination is common in commercially available raw meat diets, suggesting that there is a risk of foodborne illness in dogs fed these diets as well possible risk for humans associated with the dogs or their environments.
Animal Population Health Institute, Colorado State University, Fort Collins, CO 80523, USA.
Rachel A
RA
Strohmeyer
Paul S
PS
Morley
Doreene R
DR
Hyatt
David A
DA
Dargatz
A Valeria
AV
Scorza
Michael R
MR
Lappin
D000821:Animal Feed; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D015169:Colony Count, Microbial / Q000662:veterinary; D003257:Consumer Product Safety; D016054:DNA, Protozoan / Q000302:isolation & purification; D004285:Dogs; D056890:Eukaryota / Q000145:classification / Q000302:isolation & purification; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D016266:Food Parasitology; D008137:Longitudinal Studies; D008460:Meat / Q000382:microbiology* / Q000469:parasitology*; D011446:Prospective Studies
D016054:DNA, Protozoan