Analysis of chicken serum proteome and differential protein expression during development in single-comb White Leghorn hens.
Serum is believed to harbor thousands of distinct proteins that are either actively secreted or leak from various blood cells or tissues. Exploring protein composition in serum may accelerate the discovery of novel protein biomarkers for specific economic traits in livestock species. This study analyzed serum protein composition to establish a 2-DE reference map, and monitored protein dynamics of single-comb White Leghorn hens at 8, 19 and 23 weeks after hatching. A total of 119 CBB-stained and 315 silver-stained serum protein spots were analyzed by MALDI-TOF MS. Of these, 98 CBB-stained and 94 silver-stained protein spots were significantly matched to existing chicken proteins. The identified spots represented 30 distinctive proteins in the serum of laying hens. To compare protein expression during development, expression levels of 47 protein spots were quantified by relative spot volume with Melanie 3 software. Ten protein spots increased and 3 protein spots decreased as hen age increased. Previous research has suggested that some of these proteins play critical roles in egg production. The differentially expressed proteins with unknown identities will be valuable candidates for further explorations of their roles in egg production of laying hens.
Division of Biotechnology, Animal Technology Institute Taiwan, Taiwan, R. O. C [corrected]
San-Yuan
SY
Huang
Jyh-Hung
JH
Lin
Yu-Huey
YH
Chen
Chin-Kai
CK
Chuang
Yu-Fang
YF
Chiu
Ming-Yu
MY
Chen
Hsin-Hsin
HH
Chen
Wen-Chuan
WC
Lee
D000367:Age Factors; D000818:Animals; D030161:Avian Proteins / Q000096:biosynthesis* / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification; D001798:Blood Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens / Q000254:growth & development*; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D030161:Avian Proteins; D001798:Blood Proteins; D020543:Proteome