[Allelic polymorphism of genes encoding catalytic immunoproteasome subunits and its functional meaning].
Proteasomal activity in isolated monocytes from subjects with different variants of large multifunctional proteases genes -LMP2 (Arg60-->His allelic polymorphism) and LMP7 (Lys145-->Gln allelic polymorphism) was determined. Trypsin-like activity of proteasome was 1.6-times (P = 0.19) higher at Arg/Arg genotype comparing to His/His genotype, and 2-fold lower than at Arg/His genotype. The highest chymotrypsin-like activity of proteasome was observed in heterozygotes (on 29.7% higher comparing to Arg/Arg genotype, P = 0.43) and lowest in homozygotes His/His (on 29.7% less comparing to Arg/Arg genotype, P = 0.40). Level of RNA expression in isolated monocytes detetermined by use of RT-PCR did not differed significantly in subjects with different genotypes. Data obtained indicate that LMP2 allelic polymorphism impact peptidase activity of immunoproteasome.
V Ie
VIe
Dosenko
D V
DV
Mykhal'chuk
V Iu
VIu
Zahoriĭ
M V
MV
Khaĭtovych
O O
OO
Moĭbenko
D000483:Alleles; D020134:Catalytic Domain; D003546:Cysteine Endopeptidases / Q000235:genetics*; D004247:DNA / Q000032:analysis; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics*; D010447:Peptide Hydrolases / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D046988:Proteasome Endopeptidase Complex; D020133:Reverse Transcriptase Polymerase Chain Reaction
D009097:Multienzyme Complexes; C071313:LMP-2 protein; D004247:DNA; D010447:Peptide Hydrolases; D003546:Cysteine Endopeptidases; C077436:LMP7 protein; D046988:Proteasome Endopeptidase Complex