Transcriptional analysis of protective antigen-stimulated PBMC from non-human primates vaccinated with the anthrax vaccine absorbed.
The transcriptional responses in recombinant protective antigen (PA)-stimulated peripheral blood mononuclear cells (PBMCs) from Anthrax Vaccine Absorbed (AVA)-vaccinated rhesus macaques were evaluated using Affymetrix HGU133 Plus 2.0 GeneChips. PBMCs from animals vaccinated at 0, 4, and 26 weeks were harvested at week 30, stimulated with PA, and RNA isolated. The expression of 295 unigenes was significantly increased in PA-stimulated compared to non-stimulated PBMCs; no significant decrease in gene expression was observed. These upregulated transcripts encoded for proteins functioning in both innate and adaptive immunity. Results were corroborated for several genes by real-time RT-PCR. This study provides information on the potential underlying transcriptional mechanisms in the immune response to PA in AVA-vaccinated rhesus macaques.
Rogers
James V
JV
Battelle Memorial Institute, 505 King Avenue, JM-7, Columbus, OH 43201, USA.
Casbohm
Stacy L
SL
Giannunzio
Lenore F
LF
Sinnott
Loraine T
LT
Rust
Steven W
SW
Brys
April M
AM
Durnford
Joyce M
JM
Quinn
Conrad P
CP
Robinson
David M
DM
Hunt
Robert E
RE
Estep
James E
JE
Sabourin
Carol L K
CL
D000818:Animals; D022122:Anthrax Vaccines / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D015703:Antigens, CD / Q000235:genetics; D001427:Bacterial Toxins / Q000276:immunology*; D020869:Gene Expression Profiling; D007113:Immunity, Innate; D007372:Interferons / Q000502:physiology; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008253:Macaca mulatta; D011971:Receptors, Immunologic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051193:Toll-Like Receptors / Q000235:genetics; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*
D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D015703:Antigens, CD; D001427:Bacterial Toxins; C105299:LILRB1 protein, human; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D011971:Receptors, Immunologic; D051193:Toll-Like Receptors; D014614:Vaccines, Synthetic; C030325:anthrax toxin; D007372:Interferons