Gene expression analysis using filter cDNA microarrays.
The analysis of gene expression patterns by filter-based complementary (c)DNA microarray remains an important technique in the molecular biology laboratory, despite the development of large-scale cDNA microarray analysis (see Chapter 27). This chapter provides an overview of the methods necessary to carry out the production of membrane-based cDNA arrays and the subsequent synthesis and hybridization of radiolabeled cDNA probes.
The Breakthrough Toby Robins Breast Research Centre at the Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Peter
P
Simpson
Chris
C
Jones
Alan
A
Mackay
Sunil R
SR
Lakhani
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods