Erythropoietin-induced proliferation of gastric mucosal cells.
OBJECTIVE
To analyze the localization of erythropoietin receptor on gastric specimens and characterize the effects of erythropoietin on the normal gastric epithelial proliferation using a porcine gastric epithelial cell culture model.


METHODS
Erythropoietin receptor was detected by RT-PCR, Western blotting and immunohistochermistry. Growth stimulation effects of erythropoietin on cultured gastric mucosal cells were determined by ELISA using bromodeoxyuridine (BrdU).


RESULTS
Erythropoietin receptor was detected on cultured porcine gastric mucosal epithelial cells. Erythropoietin receptor was also detected histochemically at the base of gastric mucosal epithelium. BrdU assay demonstrated a dose-dependent increase in growth potential of cultured porcine gastric mucosal epithelial cells by administration of erythropoietin, as well as these effects were inhibited by administration of anti- erythropoietin antibody (P<0.01).


CONCLUSIONS
These findings indicate that erythropoietin has a potential to proliferate gastric mucosal epithelium via erythropoietin receptor.
Itoh
Kazuro
K
Second Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa City, Saitama 359-8513, Japan. kazuito@ndmc.ac.jp
Sawasaki
Yoshio
Y
Takeuchi
Kyoko
K
Kato
Shingo
S
Imai
Nobuhiro
N
Kato
Yoichiro
Y
Shibata
Noriyuki
N
Kobayashi
Makio
M
Moriguchi
Yoshiyuki
Y
Higuchi
Masato
M
Ishihata
Fumio
F
Sudoh
Yushi
Y
Miura
Soichiro
S
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004921:Erythropoietin / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D007150:Immunohistochemistry; D017467:Receptors, Erythropoietin / Q000032:analysis* / Q000235:genetics; D013552:Swine
D017467:Receptors, Erythropoietin; D004921:Erythropoietin