Biosynthesis of a thermostable gellan lyase by newly isolated and characterized strain of Geobacillus stearothermophilus 98.
The thermophilic strain able to degrade gellan was isolated from Bulgarian hot spring. According to its morphological and biochemical properties and by partial sequencing of its 16S rDNA, it was classified as Geobacillus stearothermophilus. It grew in a synthetic medium with gellan as the only carbon source with a specific growth rate of 0.69 h(-1) and generation time of 60 min. The strain produced thermostable gellan lyase extracellularly during exponential phase. Its synthesis was inducible; the enzyme was not registered in culture liquid without gellan. The enzyme activity was increased tenfold in conditions of continuous cultivation compared to data from batch fermentations and enzyme productivity was almost sixfold higher. The enzyme showed optimal activity at 75 degrees C in a very large pH area 4-8.5. This enzyme is the first reported thermostable gellan lyase, its residual activity was 100% after 24 h incubation at 60 degrees C and its half-life was 60 min at 70 degrees C.
Derekova
Anna
A
Institute of Microbiology, Bulgarian Academy of Science, acad. G. Bonchev str. 26, 1113, Sofia, Bulgaria.
Sjøholm
Carsten
C
Mandeva
Rossica
R
Michailova
Lilia
L
Kambourova
Margarita
M
D001406:Bacillaceae / Q000145:classification / Q000201:enzymology*; D004795:Enzyme Stability; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D011133:Polysaccharide-Lyases / Q000096:biosynthesis*; D013045:Species Specificity
D011133:Polysaccharide-Lyases; C091406:gellan lyase