Chromatin remodeling by nucleosome disassembly in vitro.
The RSC chromatin-remodeling complex completely disassembles a nucleosome in the presence of the histone chaperone Nap1 and ATP. Disassembly occurs in a stepwise manner, with the removal of H2A/H2B dimers, followed by the rest of the histones and the release of naked DNA. RSC and related chromatin-remodeling complexes may be responsible for the removal of promoter nucleosomes during transcriptional activation in vivo.
Lorch
Yahli
Y
Department of Structural Biology, Stanford University School of Medicine, CA 94305-5126, USA.
Maier-Davis
Barbara
B
Kornberg
Roger D
RD
D000818:Animals; D042002:Chromatin Assembly and Disassembly / Q000235:genetics; D006657:Histones / Q000378:metabolism; D009707:Nucleosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D051381:Rats; D015533:Transcriptional Activation
D006657:Histones; D009707:Nucleosomes