Ultrasonic tissue characterization of photodamaged skin by scanning acoustic microscopy.
The aim of this study was to ultrasonically characterize photodamaged skin of the elderly at the microscopic level using scanning acoustic microscopy which showed two-dimentional distribution of sound speed in the skin section. We confirmed that the expression level of the elastin gene was increased in the preauricular skin (photodamaged area), compared with postauricular skin (photo-protected area). The expression level of the procollagen gene was also increased in the preauricular skin compared with postauricular skin. The preauricular skin showed higher sound speed in the papillary dermis (Grenz zone). The site of progressive solar elastosis showed a somewhat sound speed velocity than that of the Grenz zone. Immunohistochemical staining showed conserved deposition of collagen in the Grenz zone even in the more photodamaged preauricular skin. These results suggest that fibrosis in the Grenz zone compensates tissue strength with the progress of solar elastosis. The sound speed analysis of skin will provide important information on heterogeneous mechanical changes in the skin during the process of photoaging.
Miyasaka
Muneo
M
Department of Plastic Surgery, Tokai University School of Medicine, Kanagawa, Japan. miyasaka@is.icc.u-tokai.ac.jp
Sakai
Shingo
S
Kusaka
Ayumi
A
Endo
Yoko
Y
Kobayashi
Masaki
M
Kobayashi
Kazuto
K
Hozumi
Naohiro
N
Tanino
Ryuzaburo
R
D000368:Aged; D000369:Aged, 80 and over; D003094:Collagen / Q000378:metabolism; D004549:Elastin / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D033401:Microscopy, Acoustic / Q000379:methods* / Q000706:statistics & numerical data; D016133:Polymerase Chain Reaction; D011347:Procollagen / Q000235:genetics; D012867:Skin / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000528:radiation effects; D015595:Skin Aging / Q000235:genetics
D011347:Procollagen; D003094:Collagen; D004549:Elastin