Cloning of a CACTA transposon-like insertion in intron I of tomato invertase Lin5 gene and identification of transposase-like sequences of Solanaceae species.
Very few CACTA transposon-like sequences have been described in Solanaceae species. Sequence information has been restricted to partial transposase (TPase)-like fragments, and no target gene of CACTA-like transposon insertion has been described in tomato to date. In this manuscript, we report on a CACTA transposon-like insertion in intron I of tomato (Lycopersicon esculentum) invertase gene Lin5 and TPase-like sequences of several Solanaceae species. Consensus primers deduced from the TPase region of the tomato CACTA transposon-like element allowed the amplification of similar sequences from various Solanaceae species of different subfamilies including Solaneae (Solanum tuberosum), Cestreae (Nicotiana tabacum) and Datureae (Datura stramonium). This demonstrates the ubiquitous presence of CACTA-like elements in Solanaceae genomes. The obtained partial sequences are highly conserved, and allow further detection and detailed analysis of CACTA-like transposons throughout Solanaceae species. CACTA-like transposon sequences make possible the evaluation of their use for genome analysis, functional studies of genes and the evolutionary relationships between plant species.
Proels
Reinhard K
RK
Lehrstuhl für Pharmazeutische Biologie, Julius von Sachs Institut, Julius von Sachs Platz 2, D-97082 Würzburg, Germany.
Roitsch
Thomas
T
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D013286:Datura stramonium / Q000235:genetics; D017343:Genes, Plant; D007438:Introns / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D019657:Solanaceae / Q000201:enzymology / Q000235:genetics*; D011198:Solanum tuberosum / Q000235:genetics; D014026:Nicotiana / Q000235:genetics; D019895:Transposases / Q000737:chemistry*; D043324:beta-Fructofuranosidase / Q000235:genetics*
D004251:DNA Transposable Elements; D019895:Transposases; D043324:beta-Fructofuranosidase