Enzymatic cleavage of fusion proteins using immobilised protease 3C.
A strategy for efficient cleavage of fusion proteins using an immobilised protease has been developed. Protease 3C from coxsackie virus was recombinantly produced in Escherichia coli and covalently immobilised onto a solid support. Thereafter, Z(basic) tagged fusion proteins, with a specific cleavage sequence between the domains, were flown through the proteolytic column and circulated until complete cleavage. Subsequently, the processed protein solution was applied on a cation exchanger. Thereby, removal of the released, positively charged fusion tag, Z(basic), was done by adsorption to the matrix while the target proteins were recovered in the flow through. Interestingly, the columns were shown to be reusable without any measurable decrease in activity. Moreover, after storage in 4 degrees C for two months the activity was almost unaffected.
Royal Institute of Technology, AlbaNova University Center, Department of Biotechnology, Stockholm, Sweden.
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M
Hedhammar
H R
HR
Jung
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S
Hober
D000086042:3C Viral Proteases; D000327:Adsorption; D002853:Chromatography, Liquid; D003546:Cysteine Endopeptidases / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014764:Viral Proteins / Q000737:chemistry*
D004800:Enzymes, Immobilized; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases