The dual-specific binding of dengue virus and target cells for the antibody-dependent enhancement of dengue virus infection.
Using flow cytometric assay and monoclonal anti-dengue Ab, we observed that both anti-E and anti-prM Abs could enhance dengue virus infection in a concentration-dependent but serotype-independent manner. Increases were found in both the percentage of dengue-infected cells and the expression of dengue E and NS1 protein per cell. Dengue virion binding and infection were enhanced on FcR-bearing cells via the Fc-FcgammaRII pathway. Furthermore, anti-prM Ab also enhanced dengue virion binding and infection on cells lacking FcR, such as BHK-21 or A549 cells, by the mechanism of peptide (CPFLKQNEPEDIDCW)-specific binding. Anti-prM Ab cross-reacted with BHK-21 or A549 cells and recognized self-Ags such as heat shock protein 60. In summary, a novel mechanism of anti-prM Ab-mediated enhancement on dengue virus infection was found to be mediated by dual specific binding to dengue virion and to target cells, in addition to the traditional enhancement on FcR-bearing cells.
Department of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China.
Kao-Jean
KJ
Huang
Yu-Ching
YC
Yang
Yee-Shin
YS
Lin
Jyh-Hsiung
JH
Huang
Hsiao-Sheng
HS
Liu
Trai-Ming
TM
Yeh
Shun-Hua
SH
Chen
Ching-Chuan
CC
Liu
Huan-Yao
HY
Lei
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000914:Antibodies, Viral / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology; D001665:Binding Sites / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology; D003715:Dengue / Q000276:immunology* / Q000821:virology*; D003716:Dengue Virus / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D007941:Leukemia P388; D008264:Macrophages / Q000276:immunology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology; D020298:U937 Cells; D014759:Viral Envelope Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; C084188:E protein, Dengue virus type 3; D010446:Peptide Fragments; D011961:Receptors, Fc; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C084187:prM protein, Dengue virus type 3