[The measurement and analysis of visible-absorption spectrum and fluorescence spectrum of lycopene].
Using ICCD spectral detection system, the absorbency of lycopene-carbon bisulfide solution with different concentration was measured, and the result shows that in a specified range the absorption rule of lycopene solution agrees with Lambert-Beer Law. Absorption spectral wavelength shifts were measured respectively when lycopene was dissolved in acetone, normal hexane, petroleum ether, benzene, ethyl acetate, and carbon bisulfide, and comparing to acetone, different red-shift appeared when lycopene was dissolved in benzene, ethyl acetate, and carbon bisulfide when water was added in lycopene-acetone solution, t he absorbency of lycopene dropped, the fine structure of absorption spectrum became indistinct, and a new absorption peak appeared in UV. The reason for these phenomena is that the solvent molecule had different effect on lycopene molecule when lycopene was dissolved in different solvent. Using fluorecence spectrophotometer, fluorescence spectra of lycopene in different concentrations were collected, and the results show that the fluorescence spectra of lycopene were mainly in 500-680 nm. When concentration was lower than 50 microg x mL(-1), the fluorescence intensity linearly increased with increasing concentration, and when concentration was higher than 60 microg x mL(-1), the fluorescence intensity dropped because of the interaction between lycopene molecules.
Institute of Atomic and Molecular Physics, Sichuan University, Chengdu 610065, China.
Xiao-zhan
XZ
Yang
Ping
P
Li
Song-hui
SH
Dai
Da-cheng
DC
Wu
Rui-xia
RX
Li
Jian-hui
JH
Yang
Hai-bo
HB
Xiao
D002338:Carotenoids / Q000032:analysis*; D000077276:Lycopene; D012997:Solvents; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods*
D012997:Solvents; D002338:Carotenoids; D000077276:Lycopene