[Obtaining of new monoclonal antibodies against human immunoglobulins of class G, suitable for use in diagnostics of infectious diseases].
An original set of 17 clones of hybridomas-producers of monoclonal antibodies (MCAB) against Fc-fragment of human IgG has been obtained. The possibility of using horseradish peroxidase conjugates of the obtained MCAB as a part of ELISA tests systems of the company "DiaProphMed" for diagnostics of syphilis, rubella, toxoplasmosis and cytomegalovirus infections has been comparatively studied. It has been shown, that the application of peroxidase conjugates of two monoclonal antibodies (156C10 and 153H11) as the part of five diagnostic ELISA test systems provides high specificity and sensitivity.
Halkin
O Iu
OIu
Nikolaienko
I V
IV
Raievs'ka
H Ie
HIe
Mykhaĭlenko
L P
LP
Kostenko
O H
OH
Spivak
M Ia
MIa
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007239:Infections / Q000175:diagnosis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G