Cytogenetic study of 435 subfertile men: incidence and clinical features.
OBJECTIVE
To evaluate the incidence of chromosomal anomalies in a series of unselected infertile men.


METHODS
Four hundred thirty-five consecutive, unselected men with a history of several years of infertility with or without sperm count anomalies and attending our center for karyotype analysis were included in this study. Peripheral blood metaphases were analyzed by standard G and Q banding. When required, fluorescence in situ hybridization and polymerase chain reaction for analysis of specific Y chromosome regions were performed.


RESULTS
Twenty-three of 435 patients (5.2%) had an abnormal karyotype; 18 of these (4.1%) showed numerical or structural anomalies of the sex chromosomes and 5 (1.1%), anomalies of the autosomes.


CONCLUSIONS
Our data confirm the importance of karyotype analysis in infertile men, especially in those with sperm anomalies. This analysis can provide useful information also for patients with long-term infertility and considering assisted reproduction techniques.
Bertini
Veronica
V
Division of Cytogenetics and Molecular Genetics, Azienda Hospital, University of Pisa, via Roma 57, 56100 Pisa, Italy.
Simi
Paolo
P
Valetto
Angelo
A
D002869:Chromosome Aberrations; D041322:Chromosomes, Human, Y; D003582:Cytogenetics; D006801:Humans; D015994:Incidence; D007248:Infertility, Male / Q000235:genetics* / Q000473:pathology; D007621:Karyotyping; D008297:Male; D013094:Spermatozoa / Q000002:abnormalities*