In vitro storage of cedar shoot cultures under minimal growth conditions.
We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were grown on a diluted modified MS medium. The addition of 6% mannitol to the storage media affected negatively survival and multiplication capacity of the cultures. The slow-growth storage conditions used in our experiments did not induce remarkable effects on both RAPD variability and average DNA methylation in the species.
Renau-Morata
Begoña
B
Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100-Burjasot, Valencia, Spain.
Arrillaga
Isabel
I
Segura
Juan
J
D028203:Cedrus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D003470:Culture Media / Q000494:pharmacology; D019175:DNA Methylation / Q000187:drug effects; D010802:Phylogeny; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development*; D019105:Random Amplified Polymorphic DNA Technique; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media