Mutual dependence of murine fetal cutaneous regeneration and peripheral nerve regeneration.
Mammalian fetal cutaneous wounds made at certain developmental stages show complete regeneration. It is reported that wound healing in both adult and fetal skin is disrupted by denervation. Furthermore, fetal cutaneous regeneration has unique aspects such as epidermal wrinkle texture regeneration and dermal regeneration that depend on developmental stage. Therefore, we have examined the relationship of fetal cutaneous regeneration with denervation. We made cutaneous wounds on fetal mice at various developmental time points including embryonic days (E)13, E15, and E17, and compared the regenerating patterns of peripheral nerves in the skin. We found that when the fetuses are wounded at an early stage of development, peripheral nerves regenerate quicker than at later stages of development when peripheral nerve regeneration is delayed. Next, we denervated the intercostal nerves and made wounds at the denervated sites on E13 and E15. We found that epidermal wrinkling and dermal regeneration were disrupted by denervation. These findings indicate that components of fetal cutaneous regeneration and peripheral nerve regeneration are mutually dependent.
Department of Plastic and Reconstructive Surgery, Keio University School of Medicine, Tokyo, Japan. kkishi@sc.itc.keio.ac.jp
Kazuo
K
Kishi
Kyoji
K
Ohyama
Hiroko
H
Satoh
Yoshiaki
Y
Kubota
Takara
T
Tanaka
Nobuaki
N
Imanishi
Hideo
H
Nakajima
Kohki
K
Kawamura
Tatsuo
T
Nakajima
D000818:Animals; D003714:Denervation; D005260:Female; D047109:Fetal Development / Q000502:physiology; D005333:Fetus; D051379:Mice; D009416:Nerve Regeneration / Q000502:physiology*; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000473:pathology; D012038:Regeneration / Q000502:physiology*; D012867:Skin / Q000293:injuries*; D013194:Staining and Labeling; D014945:Wound Healing / Q000502:physiology*