[Intestinal mucosa protection of muscovite n ulcerative colitis in rats].
OBJECTIVE
To examine the efficacy of Muscovite on acetic acid-induced ulcerative colitis in rats, and to research the mechanisms of intestinal mucosal protection.


METHODS
Ulcerative colitis was induced in rats by intracolonic injection of 2 mL of 7% acetic acid. Rats were treated with three different doses of the Muscovite and SASP at random by intracolonic injecion, the normal saline was considered as control group. The rats were sacrificed and the colons were excised and opened longitudinally. Under a dissecting microscope, gross findings were observed and scored. MPO activity was assayed by spectrophotometry in colonic mucosa.


RESULTS
Gross finding showed that multiple ulcer with diameter more than 1 cm, surrounded with erosion, erythematous and edema in the proximal colon in ulcerative coltis. The colon from Muscovite treatment group were histopatholgically normal, with slight erosion, erythematous and edema. The colon in SASP group had small ulceration and severe erosion and edema. The score of gloss change were significant lower in Muscovite groups than that in normal saline group (P < 0.01). There were necrosis and exfoliation of mucosa, multiple cystic dilation of mucosa gland, and large number of and inflammation attenuated in Muscovite groups. There nerutrophils and vessel infiltration in ulcerative colitis. The ulceration disappeared were erosion in mucosa and inflammatory cell infiltrating into submucosa in SASP group. Compared with normal saline group, the pathological scale were significant decreased in Muscovite and SASP groups (P < 0.05). The MPO activity was significant increased in colitis tissue compared with normal group (P < 0.001). After administrating with Muscovite or SASP, the level of MPO were significant decreased (P < 0.01).


CONCLUSIONS
Muscovite has the effect of mucosal protection by attenuating the inflammation of colonic mucosa and decreasing the activity of MPO.
Department of Gastroenterology, Second Affiliated Hospital Zhejiang University College of Medicine, Hangzhou, China.
Liang-jing
LJ
Wang
Shu-jie
SJ
Chen
Jian-min
JM
Si
D019342:Acetic Acid; D000538:Aluminum Silicates / Q000494:pharmacology*; D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology*; D003106:Colon / Q000201:enzymology / Q000473:pathology*; D007413:Intestinal Mucosa / Q000201:enzymology / Q000473:pathology*; D008297:Male; D008419:Materia Medica / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D000538:Aluminum Silicates; D008419:Materia Medica; D020011:Protective Agents; C517971:muscovite; D009195:Peroxidase; D019342:Acetic Acid