[Rational evolutionary design and modification of gene: a short-path of direct evolution].
Directed evolution is a powerful means in academic study and industrial application to modified and functionally improved proteins. There have been significant and impressive advances using the artificial method. Rational Evolutionary Design utilizes structural and sequence alignment information to create new genes and proteins. Rational Evolutionary Design has recently emerged as an attractive approach for studying function of proteins. The review mainly introduced four aspects: preferred codon usage, structural and sequence alignment, key domain analysis, site-directed recombination.
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. aishengxiong@yahoo.com
Ai-Sheng
AS
Xiong
Quan-Hong
QH
Yao
Zhen
Z
Zhang
Ri-He
RH
Peng
Jing
J
Zhuang
Fang
F
Xu
Jin-Ge
JG
Liu
Hong
H
Zhu
D019020:Directed Molecular Evolution; D019143:Evolution, Molecular; D005796:Genes / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D016415:Sequence Alignment