Clovamide-type phenylpropenoic acid amides, N-coumaroyldopamine and N-caffeoyldopamine, inhibit platelet-leukocyte interactions via suppressing P-selectin expression.
N-Coumaroyldopamine and N-caffeoyldopamine are clovamide-type phenylpropenoic acid amides found in Theobroma cacao. In this article, N-coumaroyldopamine and N-caffeoyldopamine were investigated to determine their effects on P-selectin expression and platelet-leukocyte interactions in vitro and in vivo models. At the concentration of 0.05 microM, they were able to inhibit P-selectin expression on the platelets by 33 (P < 0.011) and 30% (P < 0.012), respectively. The inhibition was partially blocked by beta(2)-adrenoceptor antagonists, suggesting that beta(2) receptors are probably engaged in the inhibition. N-Caffeoyldopamine and N-coumaroyldopamine could also suppress platelet-leukocyte interactions in blood samples by 36 (P < 0.013) and 32% (P < 0.011), respectively, at the same concentration (0.05 microM). In an animal study, mice administrated orally with N-caffeoyldopamine (50 and 100 microg/35 g of body weight) also showed great reduction in the P-selectin expression and platelet-leukocyte interactions by 31 to 45% (P < 0.011) and 34 to 43% (P < 0.014), respectively. These data suggest that the clovamide-type phenylpropenoic acid amides are able to suppress platelet-leukocyte interactions via inhibiting P-selectin expression.
Phytonutrients Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA. parkj@ba.ars.usda.gov
Jae B
JB
Park
Norberta
N
Schoene
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002109:Caffeic Acids / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D003373:Coumaric Acids / Q000737:chemistry / Q000494:pharmacology*; D004298:Dopamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D019007:P-Selectin / Q000096:biosynthesis*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
D002109:Caffeic Acids; D003373:Coumaric Acids; C506152:N-caffeoyldopamine; C506151:N-coumaroyldopamine; D019007:P-Selectin; D014443:Tyrosine; C416518:clovamide; D004298:Dopamine