Differential proteome analysis of tonsils from children with chronic tonsillitis or with hyperplasia reveals disease-associated protein expression differences.
A proteomic approach has been used to establish a proteome map and differentiate between the protein composition of tonsils from patients with chronic tonsillitis (CT) and that of tonsils with hyperplasia (HPL). Two-dimensional gel analysis was performed with material from four patients with HPL and five patients with CT. An average of approximately 600 spots were detected in each gel. A total of 127 different proteins were identified in 158 spots analyzed by mass spectrometry. Our study revealed disease-associated differences between protein abundance for two protein spots, an HSP27 isoform and UMP-CMP kinase. Both protein spots were more abundant in the CT group. HSP27 ELISA was performed for 32 patients, 12 belonging to the HPL group and 20 to the CT group. ELISA could not be used to differentiate HSP27 isoforms nor to distinguish CT from HPL. HSP27 was found to migrate to two further protein spots in the 2D gels. The differently expressed HSP27 isoform migrated as the most acidic of all the HSP27 isoforms detected, indicating the highest degree of phosphorylation. The sum of all three HSP27 abundances in the gels from the CT group was not different from that of the HPL group, consistent with the ELISA results. Our results suggest that phosphorylation differences caused the observed migration differences of HSP27. Together with the UMP-CMP kinase abundance differences, we conclude that kinase and/or phosphatase activity are different in CT and HPL.
Just
Tino
T
Department of Otorhinolaryngology, Head and Neck Surgery, Medical Faculty, University of Rostock, 18055 Rostock, Germany.
Gafumbegete
Evariste
E
Gramberg
Jan
J
Prüfer
Ines
I
Mikkat
Stefan
S
Ringel
Bruno
B
Pau
Hans Wilhelm
HW
Glocker
Michael O
MO
D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D006965:Hyperplasia / Q000473:pathology*; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000032:analysis*; D009703:Nucleoside-Phosphate Kinase / Q000032:analysis*; D014066:Palatine Tonsil / Q000737:chemistry*; D010766:Phosphorylation; D020033:Protein Isoforms / Q000032:analysis; D020543:Proteome; D012680:Sensitivity and Specificity; D014069:Tonsillitis / Q000473:pathology*
D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D020033:Protein Isoforms; D020543:Proteome; C019979:cytidylate kinase; D009703:Nucleoside-Phosphate Kinase