Development and evaluation of an immunochromatographic strip for trichinellosis detection.
In the present study, an immunochromatographic strip was developed for the serological detection of trichinellosis in swine. In the strip, the excretory-secretory (ES) antigens of Trichinella labelled with colloidal gold was used as the detector, and the staphylococcal protein A (SPA) and goat anti-ES antibody were blotted on the nitrocellulose membrane for the test and control lines, respectively. The evaluation of the strip was performed by comparing 60 clinical positive blood samples detected by the artificial digestion method with 46 serum samples from pigs infected with parasites other than Trichinella and 30 serum samples of parasite-free healthy pigs. The strip was shown to be of high specificity and sensitivity that were closely correlated with those of ELISA. Furthermore, the dipstick assay based on the strip is rapid (10 min) and easy to perform with no requirement of special skill, reagent or equipment. This suggests the immunochromatographic strip is an acceptable alternative to be used in clinical laboratories lacking specialized equipment as well as for field diagnosis.
Henan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. zhanggaiping2003@yahoo.com.cn
Gai-Ping
GP
Zhang
Junq-Qing
JQ
Guo
Xuan-Nian
XN
Wang
Jun-Xing
JX
Yang
Yan-Yan
YY
Yang
Qing-Mei
QM
Li
Xue-Wu
XW
Li
Rui-Guang
RG
Deng
Zhi-Jun
ZJ
Xiao
Ji-Fei
JF
Yang
Guang-Xu
GX
Xing
Dong
D
Zhao
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000937:Antigen-Antibody Reactions; D000947:Antigens, Helminth / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011934:Reagent Strips