[A study of biological characteristic and generation of monocyte-derived dendritic cell from peripheral blood of patients with nasopharyngeal carcinoma].
OBJECTIVE
To study the differences of morphology, phenotype and function of dendritic cell (DC) derived from the healthy individuals and the patients with nasopharyngeal carcinoma (NPC).


METHODS
DC were isolated and developed from peripheral blood monocytes from 12 NPC patients and 9 healthy volunteers in vitro, and were studied in terms of shape and phenotype of CD83, CD1a,CD86, HLA-DR by flow cytometry then. MTT method was used to measured the capability of DC in mixed lymphocyte reactions (MLR).


RESULTS
The shape of DC derived from monocyte in the healthy volunteers' peripheral blood was more typical than that of NPC patients'. The expression rate of CD83, CD1a on DC from healthy volunteers was significantly higher than that from NPC patients (P < 0.05). The DC from healthy volunteers had the higher expression level of CD1a and CD83 than that from NPC patients, but not significantly( P > 0.05). DC in healthy volunteer's group could trigger proliferation of allogenous lymphocyte (P < 0.05) but it could not significantly do that in NPC patients group (P > 0.05).


CONCLUSIONS
These results reveal that the yield of DC derived from NPC patient's monocyte is lower than that from healthy individual's in peripheral blood,and the DC from NPC patient may be in an immature state and could not trigger the proliferation of lymphocyte.
Department of Otolaryngology , the First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.
Zhengbo
Z
Wei
Guangwu
G
Huang
Wensheng
W
Wen
Zhe
Z
Zhang
Ying
Y
Xie
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D009000:Monocytes; D009303:Nasopharyngeal Neoplasms / Q000097:blood / Q000276:immunology / Q000473:pathology*