Capillary electrophoresis enantioselective separation of vigabatrin enantiomers by precolumn derivatization with dehydroabietylisothiocyante and UV-vis detection.
A simple and reliable capillary electrophoresis (CE) method with UV-vis detection is presented for the enantioselective separation and determination of vigabatrin enantiomers. Dehydroabietylisothiocyante (DHAIC), a novel chiral derivatizing reagent, was used for precolumn derivatization of vigabatrin enantiomers. Optimal separation was obtained with a running buffer consisting of 50 mM Na2HPO4 (pH 9.0), 17 mM sodium dodecyl sulfate (SDS) and 25% acetonitrile. The enantiomeric separation of vigabatrin derivatives was achieved within 25 min, and the resolution was found to be 2.1. Detection was followed by direct UV absorptiometric measurements at 202 nm. A calibration curve ranging from 0.3 to 6.0 microg/ml was shown to be linear, and the limit of detection was 0.15 microg/ml. The developed method has been applied to the determination of vigabatrin enantiomers spiked in human plasma, no interferences were found from endogenous amino acids.
College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China. zhaoshulin001@163.com
Shulin
S
Zhao
Rongcan
R
Zhang
Hengshan
H
Wang
Lidong
L
Tang
Yinming
Y
Pan
D045784:Abietanes / Q000737:chemistry*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017879:Isothiocyanates / Q000737:chemistry*; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013237:Stereoisomerism; D020888:Vigabatrin / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*
D045784:Abietanes; D002021:Buffers; D017879:Isothiocyanates; C511654:dehydroabietylisothiocyante; D012967:Sodium Dodecyl Sulfate; D020888:Vigabatrin