High-performance liquid chromatographic method for determination of reversible isomers of SU5416.
SU5416 shows light-induced reversible geometric isomerism. A simple, reliable, isocratic HPLC method using an UV-vis detector at lambda(425nm) was developed. The method provides efficient (R(S)=3.5) analysis of the two isomers with retention of the isomeric integrity. Additionally, the method has linearity over a wide range (50-1000microg/mL, r(2)=0.99), is accurate (99-102%, RSD <4%), and reproducible (RSD <0.8%). The method was used for analyzing pharmaceutical samples and understanding the kinetics of SU5416 isomers in methanol. In addition, this method can be used for quantifying the non-isolatable E-isomer.
Sistla
Anand
A
Formulations Research, SUGEN Inc., 230 S. Grand Avenue, South San Francisco, CA 94080, USA. Anand.Sistla@pfizer.com
Yang
Wei-Lien
WL
Shenoy
Narmada
N
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007211:Indoles / Q000032:analysis* / Q000737:chemistry; D007536:Isomerism; D007700:Kinetics; D011758:Pyrroles / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013678:Technology, Pharmaceutical / Q000379:methods*
D007211:Indoles; D011758:Pyrroles; C116890:Semaxinib