Enhanced production of MMP-1, MMP-3, MMP-13, and RANTES by interaction of chondrocytes with autologous T cells.
It has been reported that T cells and chondrocytes interact through cell surface molecules such as MHC, CD4 or CD8 in osteoarthritis (OA) and T cells are activated. The objective of this study is to investigate the responses of chondrocyte-T cell interaction in terms of metalloprotease (MMP) and chemokine production. Articular cartilage and autologous blood were obtained from patients with OA and fracture who under went prosthetic surgery. Synovial fluid (SF) was collected from OA patients. Isolated chondrocytes were co-cultured with autologous T cells. SF cells were analyzed by immunostaining or Alcian blue staining. The production of MMP-1, MMP-3, MMP-13, and regulated on activation, normal T expressed and secreted (RANTES) was enhanced by direct co-culture compared to indirect co-culture using Transwell. Production ratio of RANTES in OA was significantly higher than non-arthritic samples. CD3 positive mononuclear cells and chondrocyte-like cells were found in SF. Chondrocyte-T cell contact was more adhesive in OA samples. These results showed the production of MMPs and RANTES was enhanced by the interaction and that chondrocyte-T cell contact was possible in vivo.
Institute of Medical Science, St. Marianna University, Sugao, 216-8512 Kawasaki, Japan. nakamura@marianna-u.ac.jp
Hiroshi
H
Nakamura
Michiaki
M
Tanaka
Kayo
K
Masuko-Hongo
Kazuo
K
Yudoh
Tomohiro
T
Kato
Moroe
M
Beppu
Kusuki
K
Nishioka
D000368:Aged; D001324:Autoantigens / Q000378:metabolism; D017252:CD3 Complex / Q000032:analysis; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000096:biosynthesis*; D019902:Chondrocytes / Q000201:enzymology* / Q000276:immunology; D018920:Coculture Techniques; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology* / Q000276:immunology*; D013582:Synovial Fluid / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
D001324:Autoantigens; D017252:CD3 Complex; D018946:Chemokine CCL5; D053509:Matrix Metalloproteinase 13; D020782:Matrix Metalloproteinases; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1