[Isolation and characterization of a DRE-binding transcription factor from Yinxin poplar (Populus alba x P. alba var. pyramidalis)].
Dehydration-Responsive Element Binding ( DREB) transcription factors, specifically binding with dehydration reponsive element (DRE), activate a variety of stress-responsive genes in plants under abiotic stresses (dehydration, high salt and low temperature). Using PCR and homologous EST search, we isolated a DREB-like gene from Yinxin poplar (Populus alba x P. alba var. pyramidalis) named PaDREB2. Yeast One-hybrid experiment demonstrated that PaDREB2 protein could function as a DREB transcription factor activating target gene expression by specifically binding to DRE cis-element. To study the expression pattern of PaDREB2, RT-PCR was carried out. And the results showed that PaDREB2 is induced by low temperature, drought and high salt.
College of Life Science, Ningxia University, Yinchuan 750021, China.
Hong-Xia
HX
Qin
Zhi-Ping
ZP
Jia
Hai-Chao
HC
Zhang
Jing-Mei
JM
Liu
Yu-Xia
YX
Song
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D055864:Droughts; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D032107:Populus / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins; D014157:Transcription Factors