Topical application of mitomycin C in combination with dexamethasone: effective delay of myringotomy closure.
The object of this study was to investigate the efficacy of topical mitomycin C (MMC) in combination with subsequent topical dexamethasone to prolong the patency of myringotomies. Bilateral instrumental myringotomies were performed in 12 rats. On both sides, a solution of MMC (2 mg/ml) was applied to the intact tympanic membrane for ten minutes before myringotomy. Dexamethasone (4 mg/ml) was applied to one tympanic membrane of each animal at days 3, 6, 9 and 14. Tympanic membranes were observed weekly for a total of 9 weeks. The mean healing period was found to be 17.5 days (range 4.5-45.5 days) in the group with MMC alone, and 32 days (range 17.5-59.5 days) in the group MMC + dexamethasone. The difference was significant. A combination of topical MMC and subsequent dexamethasone did delay the healing rate of myringotomies in rat tympanic membranes longer than MMC alone.
Department of Otorhinolaryngology and Head and Neck Surgery, University of Greifswald, Greifswald, Germany. h.kaftan@web.de
Holger
H
Kaftan
Werner
W
Hosemann
D000287:Administration, Topical; D000477:Alkylating Agents / Q000008:administration & dosage*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D003907:Dexamethasone / Q000008:administration & dosage*; D004359:Drug Therapy, Combination; D008876:Middle Ear Ventilation; D016685:Mitomycin / Q000008:administration & dosage*; D010034:Otitis Media with Effusion / Q000601:surgery; D051381:Rats; D011917:Rats, Inbred Lew; D016896:Treatment Outcome; D014432:Tympanic Membrane / Q000601:surgery; D014945:Wound Healing / Q000187:drug effects*
D000477:Alkylating Agents; D000893:Anti-Inflammatory Agents; D016685:Mitomycin; D003907:Dexamethasone