An improved method for discrimination of cell populations in tissue sections using microscopy-based multicolor tissue cytometry.
BACKGROUND
In tissue context, researchers and pathologists lack a generally applicable standard for quantitative determination of cytological parameters. Increasing knowledge of disease-specific markers calls for an appropriate in situ tissue cytometry.


METHODS
Microscopy-based multicolor tissue cytometry (MMTC) permits multicolor analysis of single cells within tissue context.


RESULTS
Tissue specimens stained for CD45/CD3/CD4/CD8 were analyzed. Specificity as well as reproducibility of MMTC is demonstrated and a novel MMTC-based function to improve visual discrimination of subpopulations is introduced.


CONCLUSIONS
Our data demonstrate that MMTC constitutes an important step toward automated and quantitative fluorometry of solid tissues and cell monolayers.
Ecker
Rupert C
RC
TissueGnostics GmbH, Vienna, Austria. rupert.ecker@tissuegnostics.com
Rogojanu
Radu
R
Streit
Marc
M
Oesterreicher
Katja
K
Steiner
Georg E
GE
D000465:Algorithms; D015703:Antigens, CD / Q000032:analysis; D005057:Eukaryotic Cells / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D047569:Laser Scanning Cytometry / Q000379:methods; D017493:Leukocyte Common Antigens / Q000032:analysis; D007962:Leukocytes / Q000737:chemistry / Q000166:cytology; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000166:cytology; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation
D015703:Antigens, CD; D017493:Leukocyte Common Antigens