A new summarization method for Affymetrix probe level data.
BACKGROUND
We propose a new model-based technique for summarizing high-density oligonucleotide array data at probe level for Affymetrix GeneChips. The new summarization method is based on a factor analysis model for which a Bayesian maximum a posteriori method optimizes the model parameters under the assumption of Gaussian measurement noise. Thereafter, the RNA concentration is estimated from the model. In contrast to previous methods our new method called 'Factor Analysis for Robust Microarray Summarization (FARMS)' supplies both P-values indicating interesting information and signal intensity values.


RESULTS
We compare FARMS on Affymetrix's spike-in and Gene Logic's dilution data to established algorithms like Affymetrix Microarray Suite (MAS) 5.0, Model Based Expression Index (MBEI), Robust Multi-array Average (RMA). Further, we compared FARMS with 43 other methods via the 'Affycomp II' competition. The experimental results show that FARMS with default parameters outperforms previous methods if both sensitivity and specificity are simultaneously considered by the area under the receiver operating curve (AUC). We measured two quantities through the AUC: correctly detected expression changes versus wrongly detected (fold change) and correctly detected significantly different expressed genes in two sets of arrays versus wrongly detected (P-value). Furthermore FARMS is computationally less expensive then RMA, MAS and MBEI.


BACKGROUND
The FARMS R package is available from http://www.bioinf.jku.at/software/farms/farms.html.


BACKGROUND
http://www.bioinf.jku.at/publications/papers/farms/supplementary.ps
Department of Electrical Engineering and Computer Science, Technische Universität Berlin, 10587 Berlin, Germany. hochreit@bioinf.jku.at
Sepp
S
Hochreiter
Djork-Arné
DA
Clevert
Klaus
K
Obermayer
D000465:Algorithms; D003198:Computer Simulation; D015342:DNA Probes / Q000235:genetics*; D020869:Gene Expression Profiling; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
D015342:DNA Probes