Coupling of permeabilized microorganisms for efficient enantioselective reduction of ketone with cofactor recycling.
A novel, simple and efficient cofactor recycling method for enantioselective bioreduction has been developed by the use of permeabilized cells of a reductase-containing microorganism and a glucose dehydrogenase-containing microorganism
Zhang
Jie
J
Institute of Biotechnology, ETH-Hönggerberg, CH-8093, Zurich, Switzerland.
Witholt
Bernard
B
Li
Zhi
Z
D001407:Bacillus / Q000166:cytology / Q000378:metabolism*; D001692:Biological Transport; D001711:Biotransformation; D002463:Cell Membrane Permeability / Q000502:physiology*; D003067:Coenzymes / Q000378:metabolism*; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism*; D007659:Ketones / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D013237:Stereoisomerism; D013997:Time Factors
D003067:Coenzymes; D007659:Ketones; D010088:Oxidoreductases; D042843:Glucose 1-Dehydrogenase