Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces.
We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phiC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.
Institute of Genetics, University of Nottingham Nottingham NG7 2UH, UK.
Gholam
G
Khodakaramian
Sarah
S
Lissenden
Bertolt
B
Gust
Laura
L
Moir
Paul A
PA
Hoskisson
Keith F
KF
Chater
Margaret C M
MC
Smith
D001435:Bacteriophages / Q000235:genetics*; D017353:Gene Deletion; D005798:Genes, Bacterial; D005819:Genetic Markers; D019426:Integrases / Q000378:metabolism*; D011995:Recombination, Genetic; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000821:virology; D014764:Viral Proteins / Q000378:metabolism*
D005819:Genetic Markers; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases