The expression patterns of Toll-like receptors in the ileal pouch mucosa of postoperative ulcerative colitis patients.
The aim of this study was to evaluate the expression pattern of Toll-like receptors (TLRs) in the pouch mucosa of ulcerative colitis patients in comparison with that in the ileum mucosa of noninflammatory bowel disease patients. Pouch mucosal biopsy specimens were collected from postoperative patients who had undergone surgery for ulcerative colitis. Normal ileum specimens were collected from colon cancer patients. The specimens were assessed by immunofluorescence histochemistry using TLR2, TLR3, TLR4, and TLR5 polyclonal antibodies. The normal ileal mucosa constitutively expressed TLR3 and TLR5, whereas TLR2 and TLR4 were barely detectable. In the mucosa of active pouchitis, TLR2 and TLR4 was strongly upregulated, and TLR4 was upregulated even in a noninflamed pouch. No TLR3 or TLR5 expression was detectable. These data suggest that pouchitis may be associated with distinctive changes in selective TLR expression in the pouch mucosa, and that TLR4 alterations in the innate response system may contribute to the pathogenesis of these disorders in particular.
Second Department of Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan.
Yuji
Y
Toiyama
Toshimitsu
T
Araki
Shigeyuki
S
Yoshiyama
Jun-ichiro
J
Hiro
Chikao
C
Miki
Masato
M
Kusunoki
D003093:Colitis, Ulcerative / Q000601:surgery*; D003110:Colonic Neoplasms / Q000737:chemistry; D039021:Colonic Pouches; D005455:Fluorescent Antibody Technique; D006801:Humans; D007082:Ileum / Q000737:chemistry; D007413:Intestinal Mucosa / Q000737:chemistry*; D011184:Postoperative Period; D019449:Pouchitis / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000032:analysis; D051196:Toll-Like Receptor 3 / Q000032:analysis; D051197:Toll-Like Receptor 4 / Q000032:analysis; D051198:Toll-Like Receptor 5 / Q000032:analysis; D051193:Toll-Like Receptors / Q000032:analysis*
C495342:TLR2 protein, human; C495344:TLR3 protein, human; C495345:TLR4 protein, human; C495346:TLR5 protein, human; D051195:Toll-Like Receptor 2; D051196:Toll-Like Receptor 3; D051197:Toll-Like Receptor 4; D051198:Toll-Like Receptor 5; D051193:Toll-Like Receptors