MiniX-STR multiplex system population study in Japan and application to degraded DNA analysis.
We sought to evaluate a more effective system for analyzing X-chromosomal short tandem repeats (X-STRs) in highly degraded DNA. To generate smaller amplicon lengths, we designed new polymerase chain reaction (PCR) primers for DXS7423, DXS6789, DXS101, GATA31E08, DXS8378, DXS7133, DXS7424, and GATA165B12 at X-linked short tandem repeat (STR) loci, devising two miniX-multiplex PCR systems. Among 333 Japanese individuals, these X-linked loci were detected in amplification products ranging in length from 76 to 169 bp, and statistical analyses of the eight loci indicated a high usefulness for the Japanese forensic practice. Results of tests on highly degraded DNA indicated the miniX-STR multiplex strategies to be an effective system for analyzing degraded DNA. We conclude that analysis by the current miniX-STR multiplex systems offers high effectiveness for personal identification from degraded DNA samples.
Asamura
H
H
Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano, Japan. asamura@sch.md.shinshu-u.ac.jp
Sakai
H
H
Kobayashi
K
K
Ota
M
M
Fukushima
H
H
D044466:Asian People / Q000235:genetics; D041321:Chromosomes, Human, X; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting; D017931:DNA Primers; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D007564:Japan; D008297:Male; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction
D017931:DNA Primers