Enzymatic hydrolysis of green tea seed extract and its activity on 5alpha-reductase inhibition.
Two kaempferol glycosides were isolated from green tea seed extract (GTSE). After conducting a structure analysis, these two compounds were identified as kaempferol-3-O-[2-O-beta-D-galactopyranosyl-6-O-alpha-L-rhamnopyranosyl]-beta-D-glucopyranoside (compound 1) and kaempferol-3-O-[2-O-beta-D-xylopyranosyl-6-O-alpha-L-rhanmopyranosyl]-beta-D-glucopyranoside (compound 2). These two compounds were hydrolysed by o-glycolytic enzymes for the production of kaempferol. After performing several reactions, we found the optimum enzyme combination, a reaction with beta-galactosidase and hesperidinase. Finally, we produced kaempferol of above 95% purity. The 5alpha-reductase inhibition activities of GTSE hydrolysate (GTSE-H) containing kaempferol were evaluated by the contact cell-based metabolic method using a stable HEK 293 cell line. GTSE-H showed a good inhibition effect on HEK 293 cell lines both type 1 and type 2 on 5alpha-reductase. Especially, GTSE-H inhibited type 2 with kaempferol content dependency. The results indicate that the inhibition activity of hydrolysate on 5alpha-reductase type 2 increases in accordance with kaempferol content.
Park
Jun-Seong
JS
Skin Research Institute, AMOREPACIFIC R&D Center, Korea.
Yeom
Myeong-Hoon
MH
Park
Won-Seok
WS
Joo
Kyung-Mi
KM
Rho
Ho-Sik
HS
Kim
Duck Hee
DH
Chang
Ih Seop
IS
D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003116:Color; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D006868:Hydrolysis; D015394:Molecular Structure; D010088:Oxidoreductases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D013662:Tea / Q000737:chemistry*
D004791:Enzyme Inhibitors; D005419:Flavonoids; D010936:Plant Extracts; D013662:Tea; D010088:Oxidoreductases