Building addressable libraries: site selective coumarin synthesis and the "real-time" signaling of antibody-coumarin binding.
[reaction: see text] The feasibility of using active semiconductor chips containing addressable arrays of microelectrodes for the "real-time" monitoring of biologically relevant binding events has been demonstrated by detecting the binding of a coumarin substrate by an anticoumarin antibody. The coumarin substrate was synthesized proximal to predetermined electrodes on the chip with the use of a Pd(II) reagent that was itself generated by using the selected electrodes. Once the coumarin was synthesized, its binding to the anticoumarin antibody was detected by monitoring the current associated with a ferrocene-ferrocinium ion redox cycle that was established between the electrodes on the chip and a remote auxiliary electrode.
Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.
Eden
E
Tesfu
Kris
K
Roth
Karl
K
Maurer
Kevin D
KD
Moeller
D000906:Antibodies / Q000378:metabolism*; D020650:Combinatorial Chemistry Techniques; D003374:Coumarins / Q000138:chemical synthesis* / Q000378:metabolism; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010165:Palladium / Q000737:chemistry
D000906:Antibodies; D003374:Coumarins; D010165:Palladium; C030123:coumarin