DNA hydrolysis promoted by 1,7-dimethyl-1,4,7,10-tetraazacyclododecane.
Several acyclic and macrocyclic polyamines were evaluated for their ability to cleave DNA. 1,7-Dimethyl-1,4,7,10-tetraazacyclododecane (DMC) could hydrolyze double-strand DNA at a concentration of 25microM. pH 7.2 was the optimal condition to cleave DNA in the presence of DMC. Supercoiled DNA hydrolytic cleavage by DMC was supported by the evidence from free radical quenching and T4 ligase ligation.
College of Chemistry and Molecular Sciences, Wuhan University, Hubei, Wuhan 430072, PR China.
Shu-Hui
SH
Wan
Feng
F
Liang
Xiao-Qin
XQ
Xiong
Li
L
Yang
Xiao-Jun
XJ
Wu
Ping
P
Wang
Xiang
X
Zhou
Cheng-Tai
CT
Wu
D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006571:Heterocyclic Compounds / Q000494:pharmacology*; D006868:Hydrolysis
C512308:1,7-dimethyl-1,4,7,10-tetraazacyclododecane; D006571:Heterocyclic Compounds; D004247:DNA