Dual mutations in the Autographa californica nucleopolyhedrovirus FP-25 and p35 genes result in plasma-membrane blebbing in Trichoplusia ni cells.
Spodoptera frugiperda cells infected with Autographa californica nucleopolyhedrovirus (AcMNPV) lacking a functional anti-apoptotic p35 protein undergo apoptosis. However, such mutants replicate normally in Trichoplusia ni (TN-368) cells. An AcMNPV plaque isolate (AcdefrT) was identified during propagation of a virus deficient in p35 in TN-368 cells. This virus exhibited enhanced budded-particle formation in TN-368 cells, but was partially defective for polyhedra production in the same cells. Virus replication in AcdefrT-infected TN-368 cells was accompanied by extensive plasma-membrane blebbing and caspase activation late in infection, both features of apoptosis. Rescue of the p35 locus of AcdefrT continued to result in a reduction in polyhedra and increase in budded virus production in TN-368 cells, but no plasma-membrane blebbing was observed. The mutation was mapped to the FP-25 gene locus. This gene mutation combined with the non-functional p35 was found to be responsible for the cell-blebbing effect observed in AcdefrT-infected TN-368 cells.
Kelly
Barbara J
BJ
School of Biological and Molecular Sciences, Oxford Brookes University, The Headington Campus, Oxford OX3 0BP, UK.|Centre for Ecology and Hydrology Oxford, Mansfield Road, Oxford OX1 3SR, UK.
King
Linda A
LA
School of Biological and Molecular Sciences, Oxford Brookes University, The Headington Campus, Oxford OX3 0BP, UK.
Possee
Robert D
RD
Centre for Ecology and Hydrology Oxford, Mansfield Road, Oxford OX1 3SR, UK.
Chapple
Susan D J
SDJ
School of Biological and Molecular Sciences, Oxford Brookes University, The Headington Campus, Oxford OX3 0BP, UK.|Centre for Ecology and Hydrology Oxford, Mansfield Road, Oxford OX1 3SR, UK.
D000818:Animals; D001483:Base Sequence; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000473:pathology*; D005814:Genes, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D009036:Moths; D009154:Mutation; D019590:Nucleocapsid Proteins / Q000235:genetics*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
C121638:FP25K protein, Autographa californica nucleopolyhedrovirus; D019590:Nucleocapsid Proteins; D014764:Viral Proteins; C081618:p35 protein, Nucleopolyhedrovirus; D053148:Caspase 3; D020169:Caspases