[VEGF-induced tubulogenesis of endothelial cells from human brain malignant glioma in the three dimentional model].
OBJECTIVE
To compare the tubulogenesis capability of malignant glioma-derived microvessel endothelial cells (GDMEC) from human brain with that of ECV304 cells in a three dimentional model and to explore the significance of GDMEC in the study on angiogenesis.


METHODS
The GDMEC were isolated from malignant gliomas of human brain and purified by selective binding to the monoclonal antibody against CD105 bound to the magnetic MACS MicroBeads. GDMEC and endothelial-like cell line ECV304 were compared with their capabilities of formatting tubule-like structure (TLS) in the three dimentional collagen matrix, with or without inducement by various concentration of vascular endothelial growth factor (VEGF).


RESULTS
The obtained GDMEC had a high purification (98%) and could be successfully cultured in vitro. GDMECs formed more TLS than ECV304 cells of the same number and at the same time points. VEGF could induce rapid formation of TLS in a dose-dependent manner, however, ECV304 cells were less response to VEGF stimulation.


CONCLUSIONS
GDMEC could maintain their endothelial characteristics and potential capability of angiogenesis. They were more response to VEGF than ECV304, therefore, more suitable for in vitro studies on tumor angiogenesis.
Jiang
Xue-feng
XF
Institute of Pathology, Southwest Hospital, Third Military Medical University, Chongying 400038, China.
Bai
Jin-si
JS
Bian
Xiu-wu
XW
Lu
Jia-you
JY
Zhao
Wen
W
Shi
Jing-quan
JQ
D001932:Brain Neoplasms / Q000098:blood supply*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology; D005910:Glioma / Q000098:blood supply*; D006801:Humans; D018189:Immunomagnetic Separation; D008833:Microcirculation / Q000473:pathology; D009389:Neovascularization, Pathologic; D042442:Vascular Endothelial Growth Factors / Q000008:administration & dosage / Q000494:pharmacology*
D042442:Vascular Endothelial Growth Factors