The odontoblast as a sensory receptor cell? The expression of TRPV1 (VR-1) channels.
Previous reports have shown the expression of several mechanosensitive ionic channels on the plasma membrane in odontoblasts, which are the cells responsible for dentin formation. The membrane characteristics of odontoblasts imply that they could play critical roles in the mechano-transduction of fluid displacement within dentinal tubules into the electrical cell signals, to carry dentin sensation to the central nervous system. However, the direct ionic mechanism underlying such a dentin nociceptive function remains unclear. In the present study, we investigated the expression of the transient receptor potential vanilloid subfamily member 1 (TRPV1) channel--which essentially contributes to the detection of pain sensation--in rat odontoblasts by immunohistochemical and nystatin perforated patch-clamp techniques. Immunohistochemical observation showed the localization of TRPV1-immunoreactions on the distal regions of odontoblast membranes. In the patch-clamp experiments, we observed capsaicin-induced inward currents that were inhibited by capsazepine, a TRPV1 channel antagonist. Our results indicate a significant expression of TRPV1 channels in odontoblasts, suggesting that odontoblasts may directly respond to noxious stimuli such as a thermal-heat stimulus, and point to the necessity for a reconsideration of the cellular mechanisms of dentin sensation based on the transmembrane ionic signals in odontoblasts.
Okumura
Reijiro
R
Department of Physiology, Tokyo Dental College, Chiba, Japan.
Shima
Kaori
K
Muramatsu
Takashi
T
Nakagawa
Kan-Ichi
K
Shimono
Masaki
M
Suzuki
Takashi
T
Magloire
Henry
H
Shibukawa
Yoshiyuki
Y
D000818:Animals; D000831:Animals, Newborn; D003804:Dentin / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D007150:Immunohistochemistry; D008564:Membrane Potentials / Q000502:physiology; D009619:Nociceptors / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D009804:Odontoblasts / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D011984:Sensory Receptor Cells / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D050916:TRPV Cation Channels / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology
D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat