[Separation of proteins by gradient pressurized capillary electrochromatography].
The separation of four proteins including RNase A, cytochrome C, lysozyme and myoglobin was investigated by reversed-phase gradient pressurized capillary electrochromatography (p-CEC) with 1.5 microm non-porous silica C18 stationary phase. This mode was compared with micro-high performance liquid chromatography (mu-HPLC) and the effects of applied voltage, stationary phase and concentration of ion-pairing agent (trifluoroacetic acid, TFA) on the gradient p-CEC were also studied. This separation was performed rapidly on a new CEC instrument Trisep 2010 GV. The results showed that the retention mechanism of proteins in p-CEC mode is based on both chromatographic partitioning and electrophoretic migration. The results also demonstrated that p-CEC may have great potential for fast and efficient separation of proteins.
Zhao
Liangjuan
L
College of Life Science, Nankai University, Tianjin 300071, China. zhao9899@sina.com
Zou
Juanjuan
J
Wang
Xiuli
X
Yan
Chao
C
Gao
Ruyu
R
Liu
Mingyu
M
Lü
Xianyu
X
D053801:Capillary Electrochromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D011506:Proteins / Q000302:isolation & purification*; D015203:Reproducibility of Results; D014269:Trifluoroacetic Acid / Q000737:chemistry
D011506:Proteins; D014269:Trifluoroacetic Acid