Comparison between ELISA and ICT- MycoDot in adult pulmonary tuberculosis.
The present study was conducted to see the diagnostic efficacy of serum anti-TB IgG antibody detection in adult pulmonary tuberculosis (TB) by ELISA and Immunochromatography test (ICT) method using commercially available diagnostic kit (Pathozyme TB complex and MycoDot test respectively). This case-control study included 48 cases of adult pulmonary TB within the age range of 15-65 years. Among them, 22 (45.9%) were AFB positive confirmed cases, 12 (25.0%) were AFB negative clinically diagnosed cases before treatment and 14 (29.1%) were clinically diagnosed cases on treatment. Age and sex matched 33 controls were also included, of which 19 (57.6%) were healthy and 14 (42.4%) were sick with non-specific pulmonary infection. Overall sensitivity and specificity of ELISA were 60.42% & 96.97% (p<0.001) and those of MycoDot as 72.92% & 93.94% (p<0.001). Rate of both ELISA and MycoDot positive were higher among clinically diagnosed cases on treatment with respective values as 71.42% and 85.71%. Respective values among AFB positive confirmed TB cases were 68.18% and 72.72%. As, both ELISA and MycoDot yielded be used as a confirmatory test for TB.
Mymensingh Medical College, Mymensingh. tushar_mmcmicro@yahoo.com
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Shamsuzzaman
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Akhter
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Shamsuzzaman
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Siddique
D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000097:blood*; D016022:Case-Control Studies; D002845:Chromatography; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007159:Immunologic Tests / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000276:immunology
D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic