JSR photolithography based microvessel scaffold fabrication and cell seeding.
A simple and inexpensive lithograph approach, in which the PMMA polymer was selected to be the substrate, the negative photoresist JSR was employed to form the microchannel structure, was adopted to fabricate the microvessel scaffold. In addition, a soft PDMS based microvessel scaffold was built by using a mold that was made up of the negative photoresist JSR. With O(2) plasma treatment, the PDMS based microvessel scaffold became more hydrophilic such that the cell culture could be easier to conduct. During cell culture, it was found that the fabricated scaffold enabled the bovine endothelial cells (BEC) to statically grow. However, the overall exchange of nutrient and oxygen was inefficient. Dynamic seeding by a novel apparatus was further conducted to have better circulation of culture medium. The bovine endothelial cells could successfully be cultivated in the microvessel scaffold by dynamic seeding.
Wang
Gou-Jen
GJ
Department of Mechanical Engineering, Taiwan. gjwang@dragon.nchu.edu.tw
Hsu
Yi-Feng
YF
Hsu
Shan-Hui
SH
Horng
Ray Hua
RH
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D008422:Materials Testing; D008833:Microcirculation / Q000166:cytology* / Q000254:growth & development*; D010781:Photography / Q000379:methods*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes