Purification and characterization of Mycobacterium tuberculosis indole-3-glycerol phosphate synthase.
Indole-3-glycerol phosphate synthase (IGPS) plays an important role in the survival of Mycobacterium tuberculosis. The trpC gene, encoding IGPS, is essential for the growth of M. tuberculosis. It was expressed at the transcriptional level in cultured M. tuberculosis. The recombinant IGPS with an added His-tag was purified. The His-tag was found to have a small effect on the biochemical properties of IGPS. IGPS is a monofunctional enzyme in M. tuberculosis. Recombinant IGPS has considerable beta-pleated sheet and is relatively compact. The enzyme activity is significantly inhibited by denaturants and antibiotics, suggesting that IGPS may be a novel potential drug target of M. tuberculosis.
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, PR China.
Yanping
Y
Yang
Min
M
Zhang
Hongmei
H
Zhang
Jianqiang
J
Lei
Ruiliang
R
Jin
Shengfeng
S
Xu
Jialing
J
Bao
Lu
L
Zhang
Honghai
H
Wang
D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000737:chemistry; D007209:Indole-3-Glycerol-Phosphate Synthase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D017433:Protein Structure, Secondary
D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D007209:Indole-3-Glycerol-Phosphate Synthase