[Multiplex fluorescence in situ hybridization in detecting complex chromosomal aberrations in myelodysplastic syndromes].
OBJECTIVE
To explore the value of multiplex fluorescence in situ hybridization (M-FISH) technique in the detection of the complex chromosomal aberrations (CCAs) in myelodysplastic syndromes (MDS).


METHODS
M-FISH was used in ten MDS patients with R-banding CCAs to refine the complex chromosomal rearrangements, the constitute of marker chromosomes, and to identify the cryptic translocations.


RESULTS
Thirty-seven kinds of structural rearrangements were detected by M-FISH including insertion, deletion, translocation and derivative chromosomes, among which 34 kinds were unbalanced rearrangements, and 3 were balanced rearrangements including t(6;22) (q21; q12), t(9; 19) (q13; p13) and t(3;5) (?; ?). Seven abnormalities in the present paper were first reported in the literature. In addition, chromosome 17 aberrations (7/10) and -5/5q - (7/10) were the two most frequent abnormalities.


CONCLUSIONS
M-FISH could refine CCAs in MDS patients, find or correct the missed or misidentified abnormalities analysed by conventional cytogenetics.
Xiao
Bing
B
Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, China.
Li
Jian-yong
JY
Pan
Jin-lan
JL
Ma
Li
L
Qiu
Hai-rong
HR
Wu
Ya-fang
YF
Xue
Yong-quan
YQ
D000293:Adolescent; D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D014178:Translocation, Genetic