Efficient inhibition of HIV-1 replication in human immature monocyte-derived dendritic cells by purified anti-HIV-1 IgG without induction of maturation.
During mucosal HIV transmission, immature dendritic cells (DCs) present in the mucosa are among the first cellular targets of the virus. Previous studies have analyzed the inhibition of HIV-1 transfer from human mature DCs to T lymphocytes by neutralizing IgG, but so far no in vitro data regarding the capacity of antibodies to inhibit HIV-1 infection of immature DCs have been reported. Here, we found an increased HIV-inhibitory activity of monoclonal IgG and purified polyclonal IgG when immature monocyte-derived dendritic cells (iMDDCs) were used as target cells instead of autologous blood lymphocytes. We showed that FcgammaRII is involved in the mechanism for inhibiting HIV-1 infection of iMDDCs by IgG, whereas no induction of maturation was detected at concentrations of IgG that result in a 90% reduction of HIV replication. After induction of FcgammaRI expression on iMDDCs by IFN-gamma, an augmentation of the HIV-inhibitory activity of IgG, related to the expression of FcgammaRI, was observed. Taken together, our results demonstrate the participation of FcgammaRs in HIV-1 inhibition by IgG when iMDDCs are the targets. We propose that IgG is able to efficiently inhibit HIV-1 replication in iMDDCs and should be one of the components to be induced by vaccination.
Institut de Virologie, 3 rue Koeberlé, F-67000 Strasbourg, France. vincent.holl@hemato-ulp.u-strasbg.fr
Vincent
V
Holl
Maryse
M
Peressin
Sylvie
S
Schmidt
Thomas
T
Decoville
Susan
S
Zolla-Pazner
Anne-Marie
AM
Aubertin
Christiane
C
Moog
D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000821:virology*; D005786:Gene Expression Regulation / Q000187:drug effects; D015483:HIV Antibodies / Q000494:pharmacology*; D015658:HIV Infections / Q000517:prevention & control* / Q000635:transmission; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007074:Immunoglobulin G; D007371:Interferon-gamma / Q000494:pharmacology; D017452:Receptors, IgG / Q000235:genetics / Q000502:physiology; D014779:Virus Replication
D015483:HIV Antibodies; D007074:Immunoglobulin G; D017452:Receptors, IgG; D007371:Interferon-gamma