The effect of dexamethasone in the pathogenesis of ligature-induced periodontal disease in Wistar rats.
The aim of this study was to evaluate, in rats, the role of the systemic use of dexamethasone in the pathogenesis of induced alveolar bone loss. In 26 female Wistar rats, ligatures were placed around the second upper molars, and the contralateral ones served as intra-group controls. Two groups were formed. The test group received 0.5 mg/kg of dexamethasone subcutaneously every third day during thirty days. The control group received the same amount of saline solution. After thirty days, the animals were sacrificed and their maxillae were removed. Sodium hypochlorite was used to prepare the specimens, and the cementum-enamel junction was stained with 1% methylene blue. Morphometric analysis of the alveolar bone loss was performed with standardized digital photographs, and the distance between the cementum-enamel junction and the alveolar bone crest was measured with the software ImageTool 3.0. Intra-examiner calibration revealed a Pearson correlation coefficient of 0.99. Statistical analysis was performed by paired or independent samplet tests, as appropriate (alpha = 0.05). Dexamethasone increased the mean alveolar bone loss in ligature-induced periodontitis in relation to the control group (0.77 and 0.61 buccally, and 0.65 and 0.56 palatally, respectively). No significant differences were observed intergroups in the teeth without ligatures. In the animal model used here, the use of dexamethasone increased the progression of ligature-induced alveolar bone loss.
Cavagni
Juliano
J
School of Dentistry, Lutheran University of Brazil.
Soletti
Ana Cristina
AC
Gaio
Eduardo José
EJ
Rösing
Cassiano Kuchenbecker
CK
D016301:Alveolar Bone Loss / Q000209:etiology* / Q000473:pathology; D000539:Alveolar Process / Q000187:drug effects; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001835:Body Weight; D003907:Dexamethasone / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D010510:Periodontal Diseases / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000893:Anti-Inflammatory Agents; D003907:Dexamethasone