PCR-RFLP authentication of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus).
PCR-RFLP analysis has been applied to the identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus). PCR amplification was carried out using a set of primers flanking a conserved region of approximately 712 base pairs from the mitochondrial 12S rRNA gene. Restriction site analysis based on sequence data from this DNA fragment permitted the selection of MseI, MboII, BslI, and ApoI endonucleases for species identification. The restriction profiles obtained when amplicons were digested with the chosen enzymes allowed the unequivocal identification of all domestic and game meat species analyzed in the present work.
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
Violeta
V
Fajardo
Isabel
I
González
Inés
I
López-Calleja
Irene
I
Martín
Pablo E
PE
Hernández
Teresa
T
García
Rosario
R
Martín
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics; D004247:DNA / Q000032:analysis / Q000737:chemistry; D003670:Deer / Q000235:genetics; D005506:Food Contamination / Q000032:analysis*; D006041:Goats / Q000235:genetics; D008460:Meat / Q000032:analysis* / Q000145:classification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics; D012756:Sheep / Q000235:genetics
D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D004247:DNA