Difference of growth-inhibitory effect of Scutellaria baicalensis-producing flavonoid wogonin among human cancer cells and normal diploid cell.
Methanol extract from cultured Scutellaria baicalensis cells inhibited the proliferation of human monocytic leukemia cell line THP-1 and human osteogenic sarcoma cell line HOS. The inhibitory effects of baicalin, baicalein and wogonin, the three major flavonoids contained in the extract, were studied. It should be noted that wogonin did not show the inhibitory effect on human fetal lung normal diploid cell line TIG-1, as compared to the inhibition observed in cancer cells. Physiological analyses in THP-1 cells showed that wogonin induced cell cycle arrest at G(2)/M phase and apoptosis. This is the first report discovering a cancer-specific apoptosis-inducing activity of wogonin.
Himeji
Mika
M
Department of Biotechnology, Faculty of Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan.
Ohtsuki
Takashi
T
Fukazawa
Harumi
H
Tanaka
Miho
M
Yazaki
Shin-Ichi
S
Ui
Sadaharu
S
Nishio
Koji
K
Yamamoto
Hisako
H
Tasaka
Kachio
K
Mimura
Akio
A
D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004171:Diploidy; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D044950:Flavanones / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology; D016195:G2 Phase / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D031364:Scutellaria baicalensis / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D006131:Growth Inhibitors; D010936:Plant Extracts; C038044:baicalin; C006680:baicalein; C085514:wogonin