[Long-term culture and differentiation of human glioma stem cells ].
OBJECTIVE
To explore the culture and subculture conditions for glioma stem cells(GSCs) and to investigate the differentiation potential of GSCs.


METHODS
The cells from human glioma were mechanically dissociated. Cells were cultured in N2 or B27 medium with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF), and they were identified by immunocytochemistry.


RESULTS
Glioma stem cells from human glioma have been successfully cultured. They formed typical neurospheres in suspension, and they could be cultured and passaged steadily in vitro. The majorities of the cells expressed vimentin and nestin, which were the markers for neural stem cells. They could differentiate into neurons and astrocytes, and express glial fibrillary acidic protein and beta III-tubulin respectively.


CONCLUSIONS
Human glioma stem cells could be cultured from gliomas in vitro, and they could differentiate into neurons and astrocytes, thus providing a basis for further studies.
Department of Neurosurgery, Chaozhou Central Hospital, Chaozhou 521021, China.
Li-ye
LY
Yang
Chao-yang
CY
Wang
Jia-kun
JK
Zheng
Jian-cheng
JC
Wen
Wen-yu
WY
Li
Xiao-ping
XP
Huang
D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D064231:Nestin; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000096:biosynthesis
D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D014746:Vimentin