Development of an indirect enzyme-linked immunosorbent assay for detecting equine serum antibodies to the lipopolysaccharide of Salmonella abortusequi.
An indirect enzyme-linked immunosorbent assay (IELISA) was developed for the detection of equine serum antibodies to lipopolysaccharide of Salmonella enterica subsp. enterica serovar Abortusequi (LPS), a causative organism of Equine Paratyphoid. The data presented demonstrates that horses immunized with S. abortusequi LPS developed antibodies detectable by the IELISA. By comparison, the tube agglutination test (TAT) did not detect antibody to S. abortusequi LPS as consistently as the IELISA. The data suggests that the IELISA may be a more suitable test for the detection of serum antibodies to S. abortusequi than the TAT.
Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield, Animal Diseases Research Institute, ADRI, Brucella Centre of Expertise, 3851 Fallowfield Road, Ottawa, Ont., Canada K2H 8P9. gall@inspecion.gc.ca
D
D
Gall
K
K
Nielsen
R M
RM
Bermudez
M C
MC
Muñoz del Real
G
G
Halbert
R
R
Groulx
F
F
Moreno
E Y
EY
Chow
S L
SL
Checkley
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D006734:Horse Diseases / Q000097:blood / Q000276:immunology* / Q000382:microbiology*; D006736:Horses; D007114:Immunization / Q000662:veterinary; D008070:Lipopolysaccharides / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D019779:Salmonella enterica / Q000276:immunology*
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides