High-performance liquid chromatographic enantioseparations on capillary columns containing monolithic silica modified with amylose tris(3,5-dimethylphenylcarbamate).
Monolithic capillary columns containing native silica were modified by in situ coating with amylose tris(3,5-dimethylphenylcarbamate) and applied for enantioseparations in capillary liquid chromatography. Capillary columns were examined for 10 standard racemic compounds in order to compare the performance of monolithic silica columns with the common, 4.6mm I.D. high-performance liquid chromatographic columns packed with particulate silica. The effects of polysaccharide coating and of the linear velocity of the mobile phase on peak performance were studied. Enantioseparations with an analysis time below 1min were achieved for some chiral analytes.
Chankvetadze
Bezhan
B
Molecular Recognition and Separation Science Laboratory, School of Chemistry, Tbilisi State University, Chavchavadze Ave 1, 028 Tbilisi, Georgia.
Yamamoto
Chiyo
C
Kamigaito
Masami
M
Tanaka
Nobuo
N
Nakanishi
Kazuki
K
Okamoto
Yoshio
Y
D000688:Amylose / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D048448:Phenylcarbamates / Q000737:chemistry*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
D048448:Phenylcarbamates; C080033:Chiralpak AD; D012822:Silicon Dioxide; D000688:Amylose; D010626:Phenylethyl Alcohol