Composition of constitutive heterochromatin of Pseudonannolene strinatii Mauriès, 1974 (Diplopoda, Spirostreptida) analyzed by AT/CG specific fluorochromes.
Triple staining with fluorochromes (DA/DAPI/CMA) and C-banding were used to characterize the composition of Pseudonannolene strinatii heterochromatin. C-banding showed C+ bands of different labeling intensity on chromosomes 1 and 2 in some cells. Fluorochrome staining revealed DAPI+ regions corresponding to the C-banding pattern, indicating that the heterochromatin of this species is abundant in AT-rich sequences.
Departamento de Biologia, IB, UNESP, Av. 24 A, 1515, 13506-900 Rio Claro, SP, Brasil. kleber_agari@yahoo.com.br
Kleber Agari
KA
Campos
Carmem Silvia
CS
Fontanetti
D000818:Animals; D001181:Arthropods / Q000235:genetics*; D002871:Chromosome Banding / Q000379:methods*; D005456:Fluorescent Dyes; D006570:Heterochromatin / Q000235:genetics*; D007621:Karyotyping
D005456:Fluorescent Dyes; D006570:Heterochromatin