Involvement of Ca(2+)-induced Ca2+ releasing system in interleukin-1beta-associated adenosine release.
Interleukin-1beta (IL-1beta) plays an important role in neuroprotective and neurodegenerative events in the central nervous system. To clarify the mechanism of controversial actions of IL-1beta, we determined the effect of IL-1beta, as well as the interaction between IL-1beta and Ca(2+)-induced Ca2+ releasing system (CICR), on adenosine releases in mice hippocampus using mini-slices method. Basal and K(+)-stimulated adenosine releases were regulated by two types of CICRs, including inositol-1,4,5-trisphosphate (IP3) receptor and ryanodine receptor. Lower concentration of IL-1beta increased both adenosine releases, whereas higher concentration did not affect their releases. The stimulatory effect of IL-1beta on basal adenosine release was reduced by removal of extracellular Ca2+ and IP3 receptor inhibitor, while the stimulatory effect of IL-1beta on K(+)-stimulated adenosine release was reduced by ryanodine receptor inhibitor. These results suggest that the potent effect of IL-1beta upon adenosine release might contribute to the neuroprotective action of IL-1beta, whereas IL-1beta-induced neurodegeneration might be due to the overload response of Ca2+ mobilization and the inactivation of adenosine exocytosis.
Department of Neuropsychiatry, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan.
Gang
G
Zhu
Motohiro
M
Okada
Shukuko
S
Yoshida
Fumiaki
F
Mori
Shinichi
S
Hirose
Koichi
K
Wakabayashi
Sunao
S
Kaneko
D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D053496:Inositol 1,4,5-Trisphosphate Receptors; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000494:pharmacology*; D047028:Macrocyclic Compounds; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010080:Oxazoles / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects* / Q000378:metabolism; D017472:Receptors, Interleukin-1 / Q000187:drug effects / Q000378:metabolism; D012430:Ruthenium Red / Q000494:pharmacology; D019837:Ryanodine Receptor Calcium Release Channel / Q000187:drug effects* / Q000378:metabolism; D012795:Sialoglycoproteins / Q000494:pharmacology
D015220:Calcium Channels; C507764:Il1rn protein, mouse; D053496:Inositol 1,4,5-Trisphosphate Receptors; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D047028:Macrocyclic Compounds; D010080:Oxazoles; D018160:Receptors, Cytoplasmic and Nuclear; D017472:Receptors, Interleukin-1; D019837:Ryanodine Receptor Calcium Release Channel; D012795:Sialoglycoproteins; C108644:xestospongin A; D012430:Ruthenium Red; D000241:Adenosine; D011188:Potassium; D002118:Calcium