LCD-based detection of enzymatic action.
By incorporating an ester-containing substrate in a self-assembled alignment layer for liquid crystal cells, the presence of a lipase (CALB) can be directly detected through its enzymatic action on the alignment layer, without the need for fluorescent labelling or enzyme assays.
Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525ED, Nijmegen, The Netherlands. J.Hoogboom@science.ru.nl
Johan
J
Hoogboom
Kelly
K
Velonia
Theo
T
Rasing
Alan E
AE
Rowan
Roeland J M
RJ
Nolte
D015374:Biosensing Techniques / Q000379:methods*; D002175:Candida / Q000201:enzymology*; D004789:Enzyme Activation; D005656:Fungal Proteins; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D050866:Liquid Crystals / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D013379:Substrate Specificity; D017971:Tin Compounds / Q000737:chemistry
D005656:Fungal Proteins; D011437:Propylamines; D012821:Silanes; D017971:Tin Compounds; C109984:indium tin oxide; D008049:Lipase; C431349:lipase B, Candida antarctica; C477625:amino-propyl-triethoxysilane