Phenotype properties of a novel spontaneously immortalized odontoblast-lineage cell line.
Here we report on the spontaneous immortalization upon serial passages of mouse fetal dental papilla cells, which present odontoblast phenotype features. The cells named odontoblast-lineage cell (OLC) produced dentin extracellular matrix proteins, such as DSP and DMP1, and maintained transcripts of various matrix components as osteopontin, BMP-4, procollagen-1, and MEPE. The addition of osteogenic differentiation medium with beta-glycerophosphate and ascorbic acid was effective for inducing calcification and mineralization in vitro in cell cultures for up to 28 days. For the first time, we investigated the expression of Lhx6 and Lhx7 genes during induced biomineralization, since these new members of LIM homeodomain proteins have been recently proposed tracking odontoblastic phenotypes. Our results indicate that beta-glycerophosphate treatment of OLC cultures decreases Lhx6 transcript levels in vitro. Our findings proved odontoblast phenotype-specificity, which demonstrates that this novel odontoblast-lineage cell line is a valuable tool for future experiments in odontology.
Arany
Szilvia
S
Department of Biochemistry, Akita University School of Medicine, Hondo 1-1-1, Akita 010-8543, Japan. aszilvia@med.akita-u.ac.jp
Nakata
Akira
A
Kameda
Takashi
T
Koyota
Souchi
S
Ueno
Yasuharu
Y
Sugiyama
Toshihiro
T
D000818:Animals; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D048430:Cell Shape; D002478:Cells, Cultured; D051180:Desmoplakins / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D020869:Gene Expression Profiling; D018398:Homeodomain Proteins / Q000235:genetics; D060850:LIM-Homeodomain Proteins; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics; D009804:Odontoblasts / Q000166:cytology* / Q000378:metabolism*; D010012:Osteogenesis; D010641:Phenotype; D010750:Phosphoproteins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014083:Tooth Germ / Q000166:cytology; D014157:Transcription Factors
D051180:Desmoplakins; C513308:Dmp1 protein, mouse; C495295:Dsp protein, mouse; D016326:Extracellular Matrix Proteins; D018398:Homeodomain Proteins; C498504:LHX6 protein, mouse; C112960:LIM homeobox protein 8; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D014157:Transcription Factors