Influence of experimental parameters on the fluorescence response and recovery of the high-performance liquid chromatography analysis of fumonisin B1.
The effect of mobile phase pH, column temperature, extraction method and derivatisation time on the fluorescence response and recovery of fumonisin B1 in corn was investigated. Column temperature and mobile phase pH were negatively and positively correlated with the fluorescence response, respectively. Use of an Ultraturrax blender for extraction resulted in higher FB1 recoveries compared to a rotary shaker. In contrast to other reports, maximum fluorescence response occurred after a derivatisation time of 8 min. These results reflect on the absolute requirement for standardisation of the aforementioned parameters.
Laboratory of Food Chemistry and Human Nutrition, Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B 9000 Gent, Belgium.
Simbarashe
S
Samapundo
Bruno
B
De Meulenaer
Nathalie
N
De Muer
Johan
J
Debevere
Frank
F
Devlieghere
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D037341:Fumonisins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D012015:Reference Standards; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature
D037341:Fumonisins; C056933:fumonisin B1