Non-destructive analysis of the nuclei of transgenic living cells using laser tweezers and near-infrared raman spectroscopic technique.
Transgenic cell lines of loblolly pine (Pinus taeda L.) were analyzed by a compact laser-tweezers-Raman-spectroscopy (LTRS) system in this investigation. A low power diode laser at 785 nm was used for both laser optical trapping of single transgenic cells and excitation for near-infrared Raman spectroscopy of the nuclei of synchronized cells, which were treated as single organic particles, at the S-phase of the cell cycle. Transgenic living cells with gfp and uidA genes were used as biological samples to test this LTRS technique. As expected, different Raman spectra were observed from the tested biological samples. This technique provides a high sensitivity and enables real-time spectroscopic measurements of transgenic cell lines. It could be a valuable tool for the study of the fundamental cell and molecular biological process by trapping single nucleus and by providing a wealth of molecular information about the nuclei of cells.
Tang
Wei
W
Department of Biology, Howell Science Complex, East Carolina University, Greenville, NC 27858-4353, USA. tangw@mail.ecu.edu
Newton
Ronald J
RJ
Xie
Chang An
CA
Li
Yong Qing
YQ
Whitley
Nicki
N
D002453:Cell Cycle / Q000502:physiology; D002467:Cell Nucleus; D007834:Lasers; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D041603:Pinus taeda / Q000166:cytology*; D030821:Plants, Genetically Modified; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*