Effects of a collagenolytic cell wall component from Fusobacterium necrophorum subsp. necrophorum on rabbit tissue-culture cells.
The effects on rabbit tissue-cultured cells of collagenolytic cell wall component (CCWC) from Fusobacterium necrophorum subsp. necrophorum were investigated. Scanning electron microscopy demonstrated that CCWC damaged the cell surfaces of the rabbit granulocytes and hepatocytes but the effects of the cells differed from each other. Granulocytes appeared smooth and morphologically irregular whereas hepatocytes looked rough and had tiny holes in the cell membranes. Differences in cell viability were observed in MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium, inner salt) assay. The findings suggest that cytotoxic activity in vivo may well contribute to the establishment of an initial injury in visceral tissues, and the action of CCWC could increase the chances of survival for an invading F. necrophorum subsp. necrophorum at the first stages of infection.
Laboratory of Animal Health, Tokyo University of Agriculture, 1737 Funako, Atsugishi, Kanagawa 243-0034, Japan. heath@qf6.so-net.ne.jp
Keisuke
K
Okamoto
Masamithu
M
Kanoe
Yukio
Y
Yaguchi
Takeshi
T
Inoue
Tadao
T
Watanabe
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D017364:Collagenases / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D005674:Fusobacterium Infections / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D005675:Fusobacterium necrophorum / Q000737:chemistry*; D006098:Granulocytes / Q000187:drug effects / Q000382:microbiology* / Q000648:ultrastructure; D022781:Hepatocytes / Q000187:drug effects / Q000382:microbiology* / Q000648:ultrastructure; D007202:Indicators and Reagents / Q000378:metabolism; D008297:Male; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D013778:Tetrazolium Salts / Q000378:metabolism
D005656:Fungal Proteins; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; D017364:Collagenases