Function of membrane protein in silica nanopores: incorporation of photosynthetic light-harvesting protein LH2 into FSM.
A high amount of functional membrane protein complex was introduced into a folded-sheet silica mesoporous material (FSM) that has nanometer-size pores of honeycomb-like hexagonal cylindrical structure inside. The photosynthetic light-harvesting complex LH2, which is a typical membrane protein, has a cylindrical structure of 7.3 nm diameter and contains 27 bacteriochlorophyll a and nine carotenoid molecules. The complex captures light energy in the anoxygenic thermophilic purple photosynthetic bacterium Thermochromatium tepidum. The amount of LH2 adsorbed to FSM was determined optically and by the adsorption isotherms of N2. The FSM compounds with internal pore diameters of 7.9 and 2.7 nm adsorbed LH2 at 1.11 and 0.24 mg/mg FSM, respectively, suggesting the high specific affinity of LH2 to the interior of the hydrophobic nanopores with a diameter of 7.9 nm. The LH2 adsorbed to FSM showed almost intact absorption bands of bacteriochlorophylls, and was fully active in the capture and transfer of excitation energy. The LH2 complex inside the FSM showed increased heat stability of the exciton-type absorption band of bacteriochlorophylls (B850), suggesting higher circular symmetry. The environment inside the hydrophobic silica nanopores can be a new matrix for the membrane proteins to reveal their functions. The silica-membrane protein adduct will be useful for the construction of new probes and reaction systems.
Division of Material Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya, Aichi 464-8602 Japan.
Ippei
I
Oda
Kotaro
K
Hirata
Syoko
S
Watanabe
Yutaka
Y
Shibata
Tsutomu
T
Kajino
Yoshiaki
Y
Fukushima
Satoshi
S
Iwai
Shigeru
S
Itoh
D000327:Adsorption; D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D002841:Chromatiaceae / Q000737:chemistry; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
C081521:B800-850 light-harvesting complex, bacteria; D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D012822:Silicon Dioxide