Cloning and functional characterization of a fatty acid synthase component FAS2 gene from Saccharomyces kluyveri.
A gene coding the alpha subunit of fatty acid synthase (FAS2) was isolated from the budding yeast Saccharomyces kluyveri. Nucleotide sequence analysis indicated that this gene, termed Sk-FAS2, coded a protein having an amino acid sequence 83% identical to the FAS2 protein of S. cerevisiae (Sc-FAS2). The Sk-FAS2 gene was able to functionally complement an S. cerevisiae fas2 disruptant. This Sk-FAS2-expressing strain was found to produce larger amounts of C18 than C16, in contrast to the Sc-FAS2-expressing fas2 strain. In addition, fusion genes of Sk-FAS2 and Sc-FAS2 were transformed into a fas2-disrupted strain of S. cerevisiae, and fatty acid analysis of these transformants suggested that the region containing the acyl carrier protein and beta-ketoacyl reductase domains of yeast FAS2 protein play an important role in determining carbon chain length of fatty acids.
Oura
Takahiro
T
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B5 Nagatsuta, 226-8501, Yokohama, Kanagawa, Japan. toura@bio.titech.ac.jp
Kajiwara
Susumu
S
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics; D000213:Acyl Carrier Protein / Q000235:genetics; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D064429:Fatty Acid Synthases / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D005231:Fatty Acids, Unsaturated / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011487:Protein Conformation; D017434:Protein Structure, Tertiary / Q000235:genetics; D012440:Saccharomyces / Q000235:genetics* / Q000378:metabolism
D000213:Acyl Carrier Protein; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005656:Fungal Proteins; D015094:3-Hydroxyacyl CoA Dehydrogenases; D064429:Fatty Acid Synthases