[Study of mycobiota in the healthy conjunctiva of diabetics who reside in the urban area of the city of São Paulo, Brazil].
OBJECTIVE
To determine the mycobiota of the healthy conjunctiva in diabetic individuals, according to diabetes type, age, sex, disease time, type of treatment, and stage of diabetic retinopathy of the individuals. To identify the anemophilus mycobiota in the sampling rooms.


METHODS
A cross-sectional study was carried out on 803 diabetics who reside in the urban area of São Paulo-SP/Brazil. Sabouraud's dextrose agar culture with chloramphenicol was used for primoisolation, and the key of De Hoog was used to identify filamentous fungi.


RESULTS
Of the evaluated diabetics, 6.6% (53/803) presented type 1 diabetes and 93.4% (750/803) type 2. The positive cultures for fungi in the conjunctiva of diabetics was 4.2% (34/803), with 1.9% (1/53) in type 1 diabetics and 4.4% (33/740) in type 2 diabetics (p=0.720). With respect to the presence or not of isolated fungi, there was no statistically significant association regarding age (p=0.575), sex (p=0.517), disease time (p=0.633), type of treatment (p=0.422), and diabetic retinopathy stage (p=0.655) of the tested individuals. The identified fungi were all filamentous: Aspergillus spp. represented 59.5% (25/42) of isolations and 47.6% (20/42) of isolated species were Aspergillus niger. Growth of anemophilus fungi occurred in the air of the room and coincidences were observed between the isolated species from the air and those from the conjunctiva.


CONCLUSIONS
Presence of mycobiota in healthy conjunctivas of diabetics was identified, with no significant association between the greater number of positive fungi isolations and the type of diabetes, age, sex, disease type, type of treatment, and stage of diabetic retinopathy. In the collection rooms, anemophilus mycobiota was identified.
Andrade
Alfredo José Muniz de
AJ
Departamento de Oftalmologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil. alfredo@andrade.med.br
Höfling-Lima
Ana Luisa
AL
Yu
Maria Cecília Zorat
MC
Godoy
Patricio
P
Gompertz
Olga Fischman
OF
Bonfim
Sabrina de Souza
Sde S
Andrade
Francisco Eudes Muniz de
FE
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003228:Conjunctiva / Q000382:microbiology*; D003430:Cross-Sectional Studies; D003922:Diabetes Mellitus, Type 1 / Q000382:microbiology*; D003924:Diabetes Mellitus, Type 2 / Q000382:microbiology*; D003930:Diabetic Retinopathy / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D003904:Mitosporic Fungi / Q000145:classification / Q000302:isolation & purification*; D014505:Urban Population