The putative ABC transporter YheH/YheI is involved in the signalling pathway that activates KinA during sporulation initiation.
The primary kinases that control the supply of phosphate to the phosphorelay are KinA and KinB, although it is not yet known what type of signal(s) activates these kinases. Our systematic study of protein-protein interactions using yeast two-hybrid analysis revealed an interaction between KinA and YheH. YheH with the preceding gene product YheI is categorized as an ABC transporter. Overexpression of yheH/yheI in the kinB mutant resulted in a reduced sporulation efficiency. Moreover, reporter assays using Spo0A approximately P dependent promoters revealed that the deficiency in sporulation is probably due to a failure in the activation of Spo0A. Our results further suggest that the N-terminal region of YheH may play an important role in sensing the signal to be delivered to the C-terminally bound KinA.
Department of Bioscience, Tokyo University of Agriculture, Sakuragaoka, Tokyo, Japan.
Sanae
S
Fukushima
Mika
M
Yoshimura
Taku
T
Chibazakura
Tsutomu
T
Sato
Hirofumi
H
Yoshikawa
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000502:physiology*; D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers / Q000737:chemistry; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology; D050939:Gene Fusion / Q000235:genetics; D005838:Genotype; D010957:Plasmids; D011494:Protein Kinases / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D013171:Spores, Bacterial / Q000166:cytology / Q000502:physiology; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000096:biosynthesis
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D017931:DNA Primers; C075521:kinA protein, Bacillus subtilis; D011494:Protein Kinases; D001616:beta-Galactosidase