Effect of polyvinylpyrrolidone-iodine liposomal hydrogel on wound microcirculation in SKH1-hr hairless mice.
OBJECTIVE
Polyvinylpyrrolidone-iodine liposomal hydrogel (PVP-ILH) is a hydrogel formulation based on polyvinylpyrrolidone-iodine (PVP-I) and liposomes. The beneficial effects of PVP-ILH on wound healing have been previously shown. The aim of this study was to investigate the effects of topically applied PVP-ILH on wound microcirculation.


METHODS
Experiments were performed on wounds in male SKH1-hr hairless mice (n = 48). Mice were randomized into five treatment groups: mice treated with polyacrylic acid (PAA) and PAA 1:10 as well as PVP-ILH and PVP-ILH 1:10. Mice treated with sodium chloride served as control. Immediately as well as 3, 7, and 14 days after wounding, intravital fluorescent microscopy (IFM) was performed to determine wound surface area and standard microcirculatory parameters.


RESULTS
Topically administered PVP-ILH reduced wound size significantly faster compared to controls. Standard microcirculatory parameters, e.g. functional capillary density (FCD) and plasma leakage, showed no differences. FCD increases in all groups after wound creation. Using PVP-ILH, a trend towards higher FCD was observed.


CONCLUSIONS
The wound model in hairless mice in combination with IFM is suitable to qualitatively assess wound microcirculation over a period of 2 weeks even after topical application of pigmented ointments. PVP-ILH showed a positive effect on dermal wound healing and wound microcirculation.
Department of Plastic and Hand Surgery, Burn Center, University Hospital Bergmannsheil, Ruhr University Bochum, Bochum, Germany. stefan.langer@ruhr-uni-bochum.de
S
S
Langer
N M
NM
Botteck
B
B
Bosse
K
K
Reimer
P M
PM
Vogt
H-U
HU
Steinau
S
S
Mueller
D000818:Animals; D001160:Arterioles / Q000187:drug effects / Q000473:pathology; D001835:Body Weight; D004195:Disease Models, Animal; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008833:Microcirculation / Q000187:drug effects*; D010952:Plasma Substitutes; D011205:Povidone / Q000494:pharmacology*; D012867:Skin / Q000098:blood supply*; D014699:Venules / Q000187:drug effects / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy / Q000503:physiopathology*
D010952:Plasma Substitutes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011205:Povidone