Diagnostic accuracy of serum asialo-alpha1-acid glycoprotein concentration for the differential diagnosis of liver cirrhosis and hepatocellular carcinoma.
BACKGROUND
Serum asialoglycoproteins concentration are increased in patients with hepatic disease. We developed an antibody-lectin sandwich assay that is sensitive and specific to measure asialo-alpha(1)-acid glycoprotein (AsAGP) concentration in human serum and evaluated it as a biochemical marker for hepatic disease.


METHODS
Serum AsAGP concentration was measured by antibody-lectin sandwich assay with 610 serum specimens of patients with hepatic disease. Serum from 41 healthy donors and 155 patients with non-hepatic disease served as negative controls. The AsAGP values were analyzed by receiver operator characteristics (ROC) curve analysis. The diagnostic accuracy of AsAGP value was compared with those of the conventional biochemical markers in the liver function test.


RESULTS
Serum AsAGP concentration in 83% of patients with liver cirrhosis (LC) and 89% of patients with hepatocellular carcinoma (HCC) was increased over the cutoff value (1.33 microg/ml), indicating that an increase of serum AsAGP concentration is restricted to LC or HCC cases. The area under curve (AUC) in the ROC curve was 0.919 for LC and 0.946 for HCC.


CONCLUSIONS
Serum AsAGP concentration exhibited good diagnostic accuracy as a biochemical marker for LC and HCC. The addition of AsAGP to conventional liver function tests may significantly improve the diagnosis and prognosis.
Kim
Kyoung A
KA
Laboratory of Cellular Signalling Modulator, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Daejeon, 305-600, Korea.
Lee
Eun Young
EY
Kang
Ji Hyun
JH
Lee
Hee Gu
HG
Kim
Jae Wha
JW
Kwon
Dur Han
DH
Jang
Ye Jin
YJ
Yeom
Young Il
YI
Chung
Tai Wha
TW
Kim
Yung Dai
YD
Yoon
Do Young
DY
Song
Eun Young
EY
D000293:Adolescent; D000328:Adult; D001212:Asialoglycoproteins / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007223:Infant; D008103:Liver Cirrhosis / Q000097:blood* / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve
D001212:Asialoglycoproteins