Removal of 1,3-dichloro2-propanol and 3-chloro1,2-propanediol by the whole cell system of pseudomonas putida DSM 437.
The removal of 1,3-dichloro-2-propanol (1,3-DCP), 3-chloro-1,2-propanediol (3-CPD) and their mixtures at concentrations up to 1,000 mg . L(-1) by the whole cell system of Pseudomonas putida DSM 437 was investigated. The 1,3-DCP removal rates ranged from 2.36 to 10.55 mg . L(-1) . h(-1); 3-CPD exhibited approximately two times higher removal rates compared to 1,3-DCP for all concentrations tested. Removal of 1,3-DCP and 3-CPD followed first-order kinetics with rate constants of 0.0109 h(-1) and 0.0206 h(-1), respectively. When the whole cell system of P. putida DSM 437 was applied to mixtures of the two halohdrins, complete removal of 1,3-DCP was achieved at 144 h while removal of 3-CPD was completed at times ranging from 72 to 144 h. Time to achieve 50% removal of both halohydrins depends on the initial concentration of each in the mixture. For 1,3-DCP, it ranged from 40.55 h at 200 mg . L(-1) to 53.28 h at 500 mg . L(-1) while the respected values for 3-CPD were 33.39 and 68.91 h.
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, 157 80, Zografou, Greece.
Diomi
D
Mamma
Eleni
E
Papadopoulou
Dimitris
D
Petroutsos
Paul
P
Christakopoulos
Dimitris
D
Kekos
D001673:Biodegradation, Environmental; D018533:Biomass; D007220:Industrial Waste; D007700:Kinetics; D016958:Pseudomonas putida / Q000737:chemistry* / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*; D018508:Water Purification / Q000379:methods*; D000517:alpha-Chlorohydrin / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000378:metabolism
D007220:Industrial Waste; C033489:1,3-dichloro-2-propanol; D000517:alpha-Chlorohydrin