[Evaluation of aminoglycoside resistance phenotypes and genotyping of acetyltransferase in Escherichia coli].
To investigate the prevalence of aminoglycoside resistance and genotyping of acetyltransferase in Escherichia coli.



Resistance phenotypes to 12 antibiotics of 44 Escherichia coli isolates were analyzed using agar dilution method and 3 aminoglycoside resistance genes aac(3)-I, II and aac(6')-I were determined by PCR method.



In 44 clinical isolates, the occurrence of ESBLs was 45.45%, resistance rates were discrepant for amikacin (18.18%), gentamicin (56.82%) and tobramycin (61.36%), the prevalence of phenotype TG (tobramycin and gentamicin) indicative of aac(3)-II production and TGA (tobramycin, gentamicin and amikacin) indicative of aac(6')-I production were 36.36% and 18.18%, respectively. The most common aminoglycoside resistance genotype of acetyltransferase was aac(3)-II (52.27%) and aac(6')-I was lower (29.55%), but no aac(3)-I was detected.



At least 2 acetyltransferase genes exist in this area i.e. aac(3)-II and aac(6')-I.
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. konghs@mail.hz.zj.cn
Hai-shen
HS
Kong
Xue-fen
XF
Li
Ju-fang
JF
Wang
Min-jin
MJ
Wu
Xiao
X
Chen
Qing
Q
Yang
D000217:Acyltransferases / Q000235:genetics*; D000583:Amikacin / Q000494:pharmacology; D000617:Aminoglycosides / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D005838:Genotype; D005839:Gentamicins / Q000494:pharmacology; D010641:Phenotype; D014031:Tobramycin / Q000494:pharmacology
D000617:Aminoglycosides; D005839:Gentamicins; D000583:Amikacin; D000217:Acyltransferases; D014031:Tobramycin