Synaptic protein UNC-13 interacts with an F-box protein that may target it for degradation by proteasomes.
UNC-13 protein participates in regulating neurotransmitter release. In Drosophila melanogaster, proteasomal degradation controls UNC-13 levels at synapses. Function of the amino-terminal region of a 207 kDa form of Caenorhabditis elegans UNC-13 is unknown. Yeast two-hybrid and secondary yeast assays identified an F-box protein that interacts with this amino-terminal region. As F-box proteins bind proteins targeted for proteasomal degradation, this protein may participate in degrading a subset of UNC-13 proteins, suggesting that different forms of UNC-13 are regulated differently. Yeast assays also identified an exonuclease, a predicted splicing factor, and a protein with coiled-coil domains, indicating that UNC-13 may affect RNA function.
Polinsky
Cristina
C
Department of Biology, Ursinus College, Collegeville, PA, USA.
Houston
Chanelle
C
Vado
Jaynine
J
Shaikh
Azizahmed
A
Kohn
Rebecca E
RE
D000818:Animals; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry* / Q000378:metabolism; D002352:Carrier Proteins; D004331:Drosophila melanogaster; D005092:Exonucleases / Q000378:metabolism; D044783:F-Box Proteins / Q000737:chemistry*; D005786:Gene Expression Regulation; D018377:Neurotransmitter Agents / Q000737:chemistry; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D019076:Transgenes; D020798:Two-Hybrid System Techniques
D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D044783:F-Box Proteins; D018377:Neurotransmitter Agents; C083141:phorbol ester binding protein; D005092:Exonucleases; D046988:Proteasome Endopeptidase Complex