Parallel analysis of biomolecules on a microfabricated capillary array chip.
This paper focused on a self-developed microfluidic array system with microfabricated capillary array electrophoresis (mu-CAE) chip for parallel chip electrophoresis of biomolecules. The microfluidic array layout consists of two common reservoirs coupled to four separation channels connected to sample injection channel on the soda-lime glass substrate. The excitation scheme for distributing a 20 mW laser beam to separation channels in an array is achieved. Under the control of program, the sample injection and separation in multichannel can be achieved through six high-voltage modules' output. A CCD camera was used to monitor electrophoretic separations simultaneously in four channels with LIF detection, and the electropherograms can be plotted directly without reconstruction by additional software. Parallel multichannel electrophoresis of series biomolecules including amino acids, proteins, and nucleic acids was performed on this system and the results showed fine reproducibility.
Shen
Zheng
Z
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
Liu
Xiaojun
X
Long
Zhicheng
Z
Liu
Dayu
D
Ye
Nannan
N
Qin
Jianhua
J
Dai
Zhongpeng
Z
Lin
Bingcheng
B
D000596:Amino Acids / Q000032:analysis; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D046228:Microarray Analysis; D009696:Nucleic Acids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D015203:Reproducibility of Results
D000596:Amino Acids; D009696:Nucleic Acids; D011506:Proteins