Measurement of activity of rho GTPases during mitosis.
The members of Rho-family GTPases regulate progression through mitosis. Rho induces the contractile ring at the equatorial cortex of the dividing cell, and thus works as a molecular switch between nuclear division and cytokinesis. Cdc42 regulates the progression from prometaphase to metaphase by stabilizing microtubule attachment to the kinetochore. These results suggest that Rho GTPases are activated at specific points in mitosis and regulate each step. Here we describe the methods to analyze the activity and regulation of Rho GTPases during mitosis.
Oceguera-Yanez
Fabian
F
Department of Pharmacology, Kyoto University, Faculty of Medicine, Japan.
Narumiya
Shuh
S
D002352:Carrier Proteins / Q000378:metabolism; D000080865:Formins; D005982:Glutathione Transferase / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
D002352:Carrier Proteins; C491464:Diap1 protein, mouse; D000080865:Formins; D020662:Guanine Nucleotide Exchange Factors; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D020764:cdc42 GTP-Binding Protein; D020744:rac GTP-Binding Proteins; D020741:rho GTP-Binding Proteins