Cranial bone regeneration using a composite scaffold of Beta-tricalcium phosphate, collagen, and autologous bone fragments.
OBJECTIVE
The aim of our study was to examine the tissue response and new bone formation induced by beta-tricalcium phosphate (beta-TCP), collagen, and autologous bone fragments with fibrin glue implanted into a cranial bone defect.


METHODS
Granulated beta-TCP and collagen from porcine skin were mixed and freeze-dried. The weight ratio of both materials was 98:2. The composite scaffold for bone regeneration was composed of this artificial material and autologous bone fragments. Five adult beagle dogs were used. A cranial bone defect (2 cmx2 cm) was created in each dog. The animals were divided into two groups. In group 1 (n=3), the cranial bone defect was closed by replacing the original free bone flap, and the residual fissure and burr holes were filled with the composite scaffold with fibrin glue. In group 2 (n=2), the bone defect was closed only by replacing the original free bone flap. Three months after the treatment, computed tomography and histologic examinations were done in all animals.


RESULTS
In all dogs in group 1, the defects were almost closed by new bone; whereas in group 2, the defects were filled with fibrous tissues instead of bone.


CONCLUSIONS
This study showed that the composite scaffold made of beta-TCP, collagen, and autologous bone fragments with fibrin glue enabled reconstruction of cranial bone defects without the usual fixation materials.
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Masanao
M
Kishimoto
Shin-ichi
S
Kanemaru
Masaru
M
Yamashita
Tatsuo
T
Nakamura
Yoshihiro
Y
Tamura
Hisanobu
H
Tamaki
Koichi
K
Omori
Juichi
J
Ito
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology*; D016025:Bone Transplantation; D002130:Calcium Phosphates / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D004285:Dogs; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology; D012886:Skull / Q000293:injuries*; D014182:Transplantation, Autologous; D014947:Wounds and Injuries / Q000628:therapy*
D018786:Bone Substitutes; D002130:Calcium Phosphates; D015718:Fibrin Tissue Adhesive; C485817:beta-tricalcium phosphate; D003094:Collagen