Distribution of secreted aspartyl proteinases using a polymerase chain reaction assay with SAP specific primers in Candida albicans isolates.
Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans strains isolated from blood cultures. SAP1-SAP3 were detected in 37 of 40 (92.5%), SAP4 were detected in 3/40 (7.5%), SAP5 in 3/40 (7.5%), SAP6 in 5/40 (12.5%) of C. albicans strains isolated from vaginal swab cultures. Sap1, Sap2 and Sap3 isoenzymes were found to be related to the vaginopathic potential of C. albicans; Sap4, Sap5 and Sap6 isoenzymes were found to be correlated with systemic infections.
Department of Microbiology, Faculty of Medicine, Gazi University, 06510 Ankara, Turkey. aysekalkanci@email.com
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Kalkanci
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Bozdayi
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Biri
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Kustimur
D016282:Aspartic Acid Endopeptidases / Q000032:analysis* / Q000235:genetics*; D001769:Blood / Q000382:microbiology; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis*; D005260:Female; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics; D005800:Genes, Fungal; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D014621:Vagina / Q000382:microbiology
D004271:DNA, Fungal; D005656:Fungal Proteins; D007527:Isoenzymes; D016282:Aspartic Acid Endopeptidases; C086390:SAP1 protein, Candida albicans; C090753:SAP2 protein, Candida; C090754:SAP3 protein, Candida albicans; C086392:SAP4 protein, Candida albicans; C090755:SAP5 protein, Candida albicans