Pranlukast reduces neutrophil but not macrophage/microglial accumulation in brain after focal cerebral ischemia in mice.
OBJECTIVE
To determine whether pranlukast, a cysteinyl leukotriene receptor-1 antagonist, exerts an anti-inflammatory effect on focal cerebral ischemia in mice.


METHODS
Focal cerebral ischemia in mice was induced by permanent middle cerebral artery occlusion (MCAO). In addition to neurological deficits, infarct volume, degenerated neurons and endogenous IgG exudation, we detected accumulation of neutrophils and macrophage/microglia in the ischemic brain tissue 72 h after MCAO. Pranlukast was ip injected 30 min before and after MCAO.


RESULTS
Pranlukast significantly attenuated neurological deficits, infarct volume, neuron degeneration and IgG exudation. Importantly, pranlukast (0.01 and 0.1 mg/kg) inhibited myeloperoxidase-positive neutrophil, but not CD11b-positive macrophage/microglial accumulation in the ischemic cortical tissue.


CONCLUSIONS
Pranlukast exerts an anti-inflammatory effect on focal cerebral ischemia in the subacute phase that is limited to neutrophil recruitment through the disrupted blood-brain barrier.
Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310031, China.
Li-sheng
LS
Chu
Er-qing
EQ
Wei
Guo-liang
GL
Yu
San-hua
SH
Fang
Yu
Y
Zhou
Meng-ling
ML
Wang
Wei-ping
WP
Zhang
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001921:Brain / Q000473:pathology*; D002545:Brain Ischemia / Q000209:etiology / Q000473:pathology*; D039481:CD11b Antigen / Q000378:metabolism; D002867:Chromones / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000378:metabolism; D020244:Infarction, Middle Cerebral Artery / Q000150:complications; D020024:Leukotriene Antagonists / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D017628:Microglia / Q000276:immunology; D018696:Neuroprotective Agents / Q000494:pharmacology; D009504:Neutrophils / Q000201:enzymology; D009195:Peroxidase / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D039481:CD11b Antigen; D002867:Chromones; D007074:Immunoglobulin G; D020024:Leukotriene Antagonists; D018696:Neuroprotective Agents; D009195:Peroxidase; C047681:pranlukast