Flow-injection enhanced chemiluminescence method for determination of ciprofloxacin in pharmaceutical preparations and biological fluids.
A novel rapid and sensitive analytical method, enhanced chemiluminescence with flow-injection sampling, is described for determination of ciprofloxacin. The method is based on the chemiluminescence reaction of the potassium permanganate-sodium thiosulfate-ciprofloxacin system. An enhanced chemiluminescence reaction was developed, and optimum conditions for CL emission were investigated. The chemiluminescence intensity was linearly dependent on ciprofloxacin concentration in the range 1.0 x 10(-8)-1.0 x 10(-5) g mL(-1). The detection limit was 4 x 10(-9) g mL(-1). The relative standard deviation was 1.8% for eleven measurements of 2.0 x 10(-7) g mL(-1) ciprofloxacin standard solution. The new method enables simple, sensitive, and rapid determination of ciprofloxacin and has been successfully used for determination of ciprofloxacin in biological fluids and in ciprofloxacin hydrochloride tablet and injection.
Sun
Han-Wen
HW
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding, 071002, People's Republic of China. hanwen@mail.hbu.edu.cn
Li
Li-Qing
LQ
Chen
Xue-Yan
XY
D000975:Antioxidants / Q000494:pharmacology; D001826:Body Fluids / Q000737:chemistry*; D002939:Ciprofloxacin / Q000032:analysis* / Q000097:blood / Q000652:urine; D017022:Flow Injection Analysis; D006801:Humans; D008163:Luminescent Measurements; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011196:Potassium Permanganate / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013885:Thiosulfates / Q000494:pharmacology
D000975:Antioxidants; D004364:Pharmaceutical Preparations; D013885:Thiosulfates; D011196:Potassium Permanganate; D002939:Ciprofloxacin; C017717:sodium thiosulfate