Antibiotic-containing hyaluronic acid gel as an antibacterial carrier: Usefulness of sponge and film-formed HA gel in deep infection.
We have developed a novel bioabsorbable antibacterial carrier using hyaluronic acid (HA) gel for prevention and treatment of orthopedic infections. In this study, we investigated the in vivo antibacterial effects of two forms of this new material, an HA gel sponge and an HA gel film. A titanium cylinder was inserted into the intramedullary cavity of each rabbit femur, along with an HA gel sponge or HA gel film containing antibiotics. The HA gel sponge contained gentamycin, vancomycin, tobramycin, or minomycin. The HA gel film contained gentamycin or vancomycin. After 0, 7, and 14 days, the rabbit bone marrow was collected, and the antibacterial activity of the HA gel was determined by agar diffusion test. As a control, we used Septocoll, a commercially available antibacterial carrier. Both the HA gel sponge and HA gel film exhibited antibacterial activity. The present results indicate that HA gel containing antibiotics is a clinically useful bioabsorbable antibacterial carrier.
The Center for Advanced Research of Biomedical Engineering, Toin University of Yokohama, 1614 Kurogane, Yokohama, 225-8502, Japan. info@toyama-ra.com
Hiroaki
H
Matsuno
Kazuo
K
Yudoh
Masamichi
M
Hashimoto
Yasukazu
Y
Himeda
Teruzo
T
Miyoshi
Kaoru
K
Yoshida
Syogo
S
Kano
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D001424:Bacterial Infections / Q000382:microbiology / Q000517:prevention & control*; D001672:Biocompatible Materials / Q000737:chemistry; D004195:Disease Models, Animal; D004337:Drug Carriers; D005782:Gels; D006614:Hindlimb; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D008297:Male; D009985:Orthopedics / Q000379:methods*; D011183:Postoperative Complications / Q000517:prevention & control*; D011817:Rabbits; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D005782:Gels; D006820:Hyaluronic Acid