Peptide-binding assays and HLA II transgenic Abeta degrees mice are consistent and complementary tools for identifying HLA II-restricted peptides.
The identification of MHC class II-restricted peptides has become a priority for the development of peptide-based prophylactic and therapeutic vaccines. The aim of this study was to assess the correlations between peptide-binding assays on purified HLA II molecules and immunization of human HLA II transgenic mice deficient in murine class II molecules (Abeta degrees ). We used as models two MHC class II-restricted peptides, one derived from the HIV Nef regulatory protein (Nef (56-68)) and the other from the Schistosoma mansoni 28-kDa glutathione-S-transferase (Sm28GST (190-211)). High correlations were found between the two approaches, which showed that the Nef (56-68) and Sm28GST (190-211) peptides may represent promiscuous ligands for HLA-DQ and for HLA-DR molecules, respectively. We suggest a rational method based on the combination of peptide-binding assays and HLA II transgenic mice experiments as consistent and complementary tools for selecting T helper epitopes.
Depil
Stéphane
S
UMR 8527 CNRS/Lille II/Institut Pasteur, Institut de Biologie, Lille, France.
Angyalosi
Gerhild
G
Moralès
Olivier
O
Delacre
Myriam
M
Delhem
Nadira
N
François
Violaine
V
Georges
Bertrand
B
Hammer
Juergen
J
Maillère
Bernard
B
Auriault
Claude
C
Pancré
Véronique
V
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D018984:Epitopes, T-Lymphocyte; D015693:Gene Products, nef / Q000276:immunology; D005802:Genes, MHC Class II; D005982:Glutathione Transferase / Q000276:immunology; D006683:HLA-DQ Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D014612:Vaccines / Q000276:immunology*
D000942:Antigens, Bacterial; D018984:Epitopes, T-Lymphocyte; D015693:Gene Products, nef; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D010446:Peptide Fragments; D014612:Vaccines; C484242:28-kilodalton glutathione S-transferase antigen, Schistosoma mansoni; D005982:Glutathione Transferase