Preparation of tumor homogenates for subsequent preparation of cytosols, membrane fractions, RNA, and DNA.
This chapter outlines methods for the preparation of several different cellular fractions from whole samples of tumor and normal tissue.
Cancer Research UK Molecular Oncology Laboratories, Weatherall Institute of Molecular Medicine, The John Radcliffe Hospital, Oxford, UK.
Naomi
N
Robertson
Russell
R
Leek
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D003600:Cytosol / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D006801:Humans; D009369:Neoplasms / Q000737:chemistry*; D012313:RNA / Q000032:analysis*
D012313:RNA; D004247:DNA