Modulation of DNA intercalation by resveratrol and genistein.
Time correlated Single Photon Counting study (TCSPC) was performed for the first time to evaluate the effect of resveratrol (RES) and genistein (GEN) at 10-100 microM and 10-150 microM respectively, in modulating the DNA conformation and the variation induced due to intercalation by the dyes, ethidium bromide (EtBr) and acridine orange (AO). It is demonstrated using UV-absorption and fluorescence spectroscopy that RES and GEN, at 50 microM and 100 microM respectively can bind to DNA resulting in significant de-intercalation of the dyes, preventing their further intercalation within DNA. Hyperchromicity with red/blue shifts in DNA when bound to dyes was reduced upon addition of RES and GEN. DNA-dependent fluorescence of EtBr and AO was quenched in the presence of RES by 87.97% and 79.13% respectively, while similar quenching effect was observed for these when interacted with GEN (85.52% and 83.85%). It is found from TCSPC analysis that the higher lifetime component or constituent of intercalated dyes (tau(2), A (2)) decreased with the subsequent increase in smaller component or constituent of free dye (tau(1), A (1)) after the interaction of drugs with the intercalated DNA. Thus these findings signify that RES and GEN can play an important role in modulating DNA intercalation, leading to the reduction in DNA-directed toxicity.
Department of Genetics, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India.
Subbiah
S
Usha
Irudayam Maria
IM
Johnson
Raghunathan
R
Malathi
D000165:Acridine Orange / Q000737:chemistry; D016588:Anticarcinogenic Agents / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D004996:Ethidium / Q000737:chemistry; D019833:Genistein / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D000077185:Resveratrol; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013267:Stilbenes / Q000737:chemistry*
D016588:Anticarcinogenic Agents; D004396:Coloring Agents; D007364:Intercalating Agents; D013267:Stilbenes; D004247:DNA; C427203:calf thymus DNA; D019833:Genistein; D004996:Ethidium; D000165:Acridine Orange; D000077185:Resveratrol