Immunohistochemical study of the distribution of sodium-dependent vitamin C transporters in adult rat brain.
Sodium-dependent vitamin C transporters (SVCTs) is known to transport the reduced form of ascorbic acid into the cell, whereas the oxidized form of vitamin C (VC) is moved through a facilitative sugar transporter, such as glucose transporter (GLUT). With regard to the distribution of SVCT1 and -2 within the various organs, they were reported to be expressed in different types of cells. Especially in the central nervous system, only SVCT2 mRNA was expressed mainly in neurons and some types of neuroglial cells. However, data on the expression of SVCT proteins in the brain are scant. Therefore, we tried to develop comprehensive data on the distribution of SVCT proteins in adult rat brain by using immunohistochemical techniques for the first time. In our study, SVCT2 immunoreactivities (IRs) were intensely localized in the neurons of cerebral cortex, hippocampus, and Purkinje cells of cerebellum, and much weaker SVCT2 IRs were found in the other brain regions. Judging from double-immunohistochemical data, most of the cells expressing SVCT2 IRs were likely to be neurons or microglia, even though the cells in choroids plexus or ependymal cells around the ventricles also exhibited SVCT2 IRs. Complete mapping of the distribution of SVCT2 IRs was available by using a semiquantitative method. The subcellular localization of SVCT proteins is necessary for understanding the exact role of the protein, so the current overall mapping of SVCT IRs in the rat brain could be the basis for further studies on related subjects.
Department of Anatomy, Seoul National University College of Medicine, Seoul, Korea.
Ga Hee
GH
Mun
Myeung Ju
MJ
Kim
Ju Hyun
JH
Lee
Hyun Jun
HJ
Kim
Yoon Hee
YH
Chung
Young Bae
YB
Chung
Jae Seung
JS
Kang
Young Il
YI
Hwang
Seung Ha
SH
Oh
Joong-Gon
JG
Kim
Douk Ho
DH
Hwang
Dong Hoon
DH
Shin
Wang Jae
WJ
Lee
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D007150:Immunohistochemistry; D017628:Microglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D029363:Organic Anion Transporters, Sodium-Dependent / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D060347:Sodium-Coupled Vitamin C Transporters; D027981:Symporters / Q000378:metabolism*
D029363:Organic Anion Transporters, Sodium-Dependent; C555166:Slc23a1 protein, rat; C555167:Slc23a2 protein, rat; D060347:Sodium-Coupled Vitamin C Transporters; D027981:Symporters