Effects of administration of Embelin and Curcumin on lipid peroxidation, hepatic glutathione antioxidant defense and hematopoietic system during N-nitrosodiethylamine/Phenobarbital-induced hepatocarcinogenesis in Wistar rats.
The effects of administration of Embelin (EMB) and Curcumin (CUR) on lipid peroxidation, hepatic glutathione antioxidant defense and hematopoietic cells were examined during N-nitrosodiethylamine (DENA-200 mg kg(-1)body wt, single I.P injection) initiated and Phenobarbital (PB-0.05% in drinking water orally for 13 weeks) promoted hepatocarcinogenesis in Wistar strain male albino rats. DENA/PB-induced hepatic damage was manifested by a significant drop in the hepatic glutathione antioxidant defense, increased lipid peroxidation and histological alterations like dysplasia, and atypical cells with abnormal chromatin pattern. Treatment with Curcumin (100 mg kg(-1)body wt) and Embelin (50 mg kg(-1)body wt) prevented the drop in hepatic glutathione antioxidant defense, decreased lipid peroxidation, minimized the histological alterations induced by DENA/PB, but showed toxic effects on the hematopoietic cells. Results indicate the beneficial effects of Embelin and Curcumin against oxidative tissue damage during chemically-induced hepatocarinogenesis in rats.
Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharati Campus, Bangalore 560 056, Karnataka, India. mpriya7@yahoo.com
Meenakshisundaram
M
Sreepriya
Geetha
G
Bali
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism*; D016227:Benzoquinones / Q000494:pharmacology*; D001772:Blood Cell Count; D002273:Carcinogens; D003474:Curcumin / Q000494:pharmacology*; D004052:Diethylnitrosamine; D004357:Drug Synergism; D005978:Glutathione / Q000378:metabolism*; D006410:Hematopoiesis / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010634:Phenobarbital; D051381:Rats; D017208:Rats, Wistar
D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D016227:Benzoquinones; D002273:Carcinogens; D004052:Diethylnitrosamine; D005978:Glutathione; D003474:Curcumin; C010945:embelin; D010634:Phenobarbital