Human U937 monocyte behavior and protein expression on various formulations of three-dimensional fibrin clots.
Fibrin sealant products such as Tisseel (Baxter Healthcare Corporation) are used in hemostasis and tissue sealing. Tisseel consists of two components, a fibrinogen-containing component and thrombin, which when mixed together form a fibrin clot. There is an interest in delivering monocytes to the wound because they are known to play an important role in the wound-healing process. Therefore, we were interested in finding the best fibrin formulation for delivering monocytes by examining monocyte behavior on 3D-fibrin clots. Using standard adhesion and proliferation assays, we found that monocytes differentially adhere, proliferate, and cluster on and within the 3D-fibrin clots depending on the final fibrinogen and thrombin concentration. Moreover, using a Boyden chamber assay, we found that monocytes migrated through the 3D-fibrin clots in 1-2 days. Furthermore, the protein expression in monocytes seeded on 3D-fibrin clots for various time points varied depending on the fibrinogen and thrombin concentrations in the final 3D-fibrin clots. The above data suggest that various formulations of fibrin sealant Tisseel present a good surface for monocyte adhesion, proliferation and migration in vitro and potentially during the wound-healing process in vivo. Moreover, because the monocytes proliferated well and clustered in the 3D fibrin, Tisseel could be a good delivery vehicle for delivering monocytes into chronic wounds to overcome a healing deficiency.
BioSurgery Division, Baxter Healthcare Corporation, Westlake Village, California 91362, USA.
Miyeko
M
Mana
Marietta
M
Cole
Steve
S
Cox
Bill
B
Tawil
D001777:Blood Coagulation / Q000187:drug effects*; D001798:Blood Proteins / Q000032:analysis*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D005337:Fibrin / Q000187:drug effects / Q000502:physiology*; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D006801:Humans; D009000:Monocytes / Q000502:physiology*; D020298:U937 Cells
D001798:Blood Proteins; D015718:Fibrin Tissue Adhesive; D005337:Fibrin