Evaluation of a direct, rapid immunohistochemical test for rabies diagnosis.
A direct rapid immunohistochemical test (dRIT) was evaluated under field and laboratory conditions to detect rabies virus antigen in frozen and glycerol-preserved field brain samples from northwestern Tanzania. Compared to the direct fluorescent antibody test, the traditional standard in rabies diagnosis, the dRIT was 100% sensitive and specific.
University of Edinburgh, Midlothian, United Kingdom.
Tiziana
T
Lembo
Michael
M
Niezgoda
Andrés
A
Velasco-Villa
Sarah
S
Cleaveland
Eblate
E
Ernest
Charles E
CE
Rupprecht
D000818:Animals; D000829:Animals, Domestic / Q000821:virology*; D000835:Animals, Wild / Q000821:virology*; D000956:Antigens, Viral / Q000032:analysis*; D001921:Brain / Q000821:virology; D002415:Cats; D002417:Cattle; D004285:Dogs; D005990:Glycerol; D007150:Immunohistochemistry / Q000379:methods*; D011159:Population Surveillance; D011818:Rabies / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D011820:Rabies virus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013636:Tanzania / Q000453:epidemiology; D013997:Time Factors; D014021:Tissue Preservation
D000956:Antigens, Viral; D005990:Glycerol