False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise.
Erythropoietin (Epo) is a glycoprotein hormone that promotes the production of red blood cells. Recombinant human Epo (rhEpo) is illicitly used to improve performance in endurance sports. Doping in sports is discouraged by the screening of athletes for rhEPO in urine. The adopted test is based on a combination of isoelectric focusing and double immunoblotting, and distinguishes between endogenous and recombinant human Epo. We show here that this widely used test can occasionally lead to the false-positive detection of rhEpo (epoetin-beta) in postexercise, protein-rich urine, probably because the adopted monoclonal anti-Epo antibodies are not monospecific.
Department of Molecular Cell Biology, Catholic University of Leuven, Belgium. mathieu.bollen@med.kuleuven.be
Monique
M
Beullens
Joris R
JR
Delanghe
Mathieu
M
Bollen
D000918:Antibody Specificity; D015153:Blotting, Western; D004921:Erythropoietin / Q000008:administration & dosage / Q000652:urine*; D015444:Exercise / Q000502:physiology*; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D010807:Physical Endurance / Q000502:physiology*; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins
D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; C103998:epoetin beta; D004921:Erythropoietin