Aberrant alternative splicing of interferon regulatory factor-1 (IRF-1) in myelodysplastic hematopoietic progenitor cells.
Interferon regulatory factor-1 (IRF-1) mRNA expression was examined in specific cell populations (BMMC, CD34+ and CD71+) derived from 45 MDS patients and 20 controls. All the MDS cell populations, presented an identical IRF-1 mRNA expression pattern, characterized by the absence of full-length IRF-1 mRNA and presence of multiple alternative transcripts. The most common deletions involved exons 2 and 3. Two novel truncated IRF-1 protein forms were detected in MDS BMMC. IRF-1-induced iNOS mRNA expression was exclusively detected in BMMC having full-length transcript. The expression of IRF-1 truncated mRNA and protein forms might be a critical event in the development of MDS.
Department of Pathophysiology, Medical School, National University of Athens, 75 M. Asias St., Goudi, 11527 Athens, Greece.
Christos I
CI
Maratheftis
Pelagia E
PE
Bolaraki
Stavroula
S
Giannouli
Efstathia K
EK
Kapsogeorgou
Haralampos M
HM
Moutsopoulos
Michael
M
Voulgarelis
D000368:Aged; D000369:Aged, 80 and over; D017398:Alternative Splicing / Q000235:genetics*; D015703:Antigens, CD; D018952:Antigens, CD34; D002478:Cells, Cultured; D005091:Exons / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D050856:Interferon Regulatory Factor-1 / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011990:Receptors, Transferrin
D015703:Antigens, CD; D018952:Antigens, CD34; C068126:CD71 antigen; D050856:Interferon Regulatory Factor-1; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D052247:Nitric Oxide Synthase Type II