CoryneRegNet: an ontology-based data warehouse of corynebacterial transcription factors and regulatory networks.
BACKGROUND
The application of DNA microarray technology in post-genomic analysis of bacterial genome sequences has allowed the generation of huge amounts of data related to regulatory networks. This data along with literature-derived knowledge on regulation of gene expression has opened the way for genome-wide reconstruction of transcriptional regulatory networks. These large-scale reconstructions can be converted into in silico models of bacterial cells that allow a systematic analysis of network behavior in response to changing environmental conditions.


METHODS
CoryneRegNet was designed to facilitate the genome-wide reconstruction of transcriptional regulatory networks of corynebacteria relevant in biotechnology and human medicine. During the import and integration process of data derived from experimental studies or literature knowledge CoryneRegNet generates links to genome annotations, to identified transcription factors and to the corresponding cis-regulatory elements. CoryneRegNet is based on a multi-layered, hierarchical and modular concept of transcriptional regulation and was implemented by using the relational database management system MySQL and an ontology-based data structure. Reconstructed regulatory networks can be visualized by using the yFiles JAVA graph library. As an application example of CoryneRegNet, we have reconstructed the global transcriptional regulation of a cellular module involved in SOS and stress response of corynebacteria.


CONCLUSIONS
CoryneRegNet is an ontology-based data warehouse that allows a pertinent data management of regulatory interactions along with the genome-scale reconstruction of transcriptional regulatory networks. These models can further be combined with metabolic networks to build integrated models of cellular function including both metabolism and its transcriptional regulation.
International NRW Graduate School in Bioinformatics and Genome Research, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstrasse 25, D-33615 Bielefeld, Germany.
Jan
J
Baumbach
Karina
K
Brinkrolf
Lisa F
LF
Czaja
Sven
S
Rahmann
Andreas
A
Tauch
D003196:Computer Graphics; D003352:Corynebacterium / Q000235:genetics* / Q000378:metabolism*; D003628:Database Management Systems; D030541:Databases, Genetic; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D023281:Genomics; D020407:Internet; D013014:SOS Response, Genetics; D012984:Software; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014584:User-Computer Interface
D014157:Transcription Factors