A combination of diagnostic tools for rapid screening of ovine listeriosis.
A combined serological and PCR method for the detection of Listeria monocytogenes infection in symptomatic and asymptomatic ovine flocks was evaluated. Seventy-eight milk samples and 157 serum samples were analysed using a L. monocytogenes PCR detection kit and an anti-listeriolysin O IgG immunoassay kit. The combined use of these commercial kits allowed a rapid and effective detection of L. monocytogenes infection in both the early stage, before seroconversion, and in a later phase, even after antibiotic therapy.
Centro di Biotecnologie, Università degli Studi di Urbino, via T. Campanella 1, 61032 Fano, PU, Italy.
G
G
Amagliani
C
C
Giammarini
E
E
Omiccioli
E G
EG
Merati
G
G
Pezzotti
G
G
Filippini
G
G
Brandi
M
M
Magnani
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001427:Bacterial Toxins / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006360:Heat-Shock Proteins / Q000737:chemistry; D006460:Hemolysin Proteins; D006651:Histocytochemistry / Q000662:veterinary; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification*; D008088:Listeriosis / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D008403:Mass Screening / Q000379:methods / Q000662:veterinary; D008892:Milk / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000382:microbiology*
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; C038705:hlyA protein, Listeria monocytogenes