Isolation and characterization of a set of disease resistance-gene analogs (RGAs) from wild rice, Zizania latifolia Griseb. I. Introgression, copy number lability, sequence change, and DNA methylation alteration in several rice-Zizania introgression lines.
Eight resistance-gene analogs (RGAs) were isolated from wild rice, Zizania latifolia Griseb., by degenerate primers designed according to conserved motifs at or around the nucleotide-binding site (NBS) of known NBS-containing plant resistance genes. The 8 RGAs were classified into 6 distinct groups based on their deduced amino acid sequence similarity of 60% or greater. Gel-blot hybridization of each of the RGAs to 4 rice - Z. latifolia intro gression lines indicated an array of changes at either introgressed Zizania RGAs or, more likely, their rice homologs. The changes included dramatic increase in copy number, modification at the primary DNA sequence, and alteration in DNA methylation patterns.
Laboratory of Plant Epigenetics, Institute of Genetics and Cytology, Northeast Normal University , Chngchun 130024, China.
Yu
Y
Chen
Likun
L
Long
Xiuyun
X
Lin
Wanli
W
Guo
Bao
B
Liu
D000595:Amino Acid Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D019175:DNA Methylation; D017931:DNA Primers / Q000737:chemistry; D044127:Epigenesis, Genetic; D007930:Leucine / Q000737:chemistry; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D007930:Leucine