Zebrafish transgenic Enhancer TRAP line database (ZETRAP).
BACKGROUND
The zebrafish, Danio rerio, is used as a model organism to study vertebrate genetics and development. An effective enhancer trap (ET) in zebrafish using the Tol2 transposon has been demonstrated. This approach could be used to study embryogenesis of a vertebrate species in real time and with high resolution.


METHODS
The information gathered during the course of systematic investigation of many ET transgenic lines have been collected and compiled in the form of an online database--the Zebrafish Enhancer TRAP lines database (ZETRAP).


CONCLUSIONS
ZETRAP is a web-based system that provides data and information to the scientific community about the developmental, genetic and genomic aspects of transgenic zebrafish lines obtained using Tol2 transposon-mediated transgenesis. The current version (version 1.0) contains description of 27 ET lines that express EGFP in various organs and tissues, for example, heart, brain, notochord, gut, etc. It also includes information on insertion sites of the Tol2 transposon in these lines.
Choo
Benjamin G H
BG
Institute of Molecular and Cell Biology, 61 Biopolis Dr, Proteos, 138673, Singapore. benjaminchoogh@yahoo.com
Kondrichin
Igor
I
Parinov
Sergey
S
Emelyanov
Alexander
A
Go
William
W
Toh
Wei-chang
WC
Korzh
Vladimir
V
D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology / Q000235:genetics / Q000254:growth & development; D004251:DNA Transposable Elements; D030541:Databases, Genetic; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000032:analysis; D020407:Internet; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000254:growth & development
D004251:DNA Transposable Elements; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins