Cdx2 modulates proliferation in normal human intestinal epithelial crypt cells.
The homeobox gene Cdx2 is involved in the regulation of the expression of intestine specific markers such as sucrase-isomaltase and lactase-phlorizin hydrolase. Previous studies performed with immortalized or transformed intestinal cell lines have provided evidence that Cdx2 can promote morphological and functional differentiation in these experimental models. However, no data exist concerning the implication of this factor in normal human intestinal cell physiology. In the present work, we have investigated the role of Cdx2 in normal human intestinal epithelial crypt (HIEC) cells that lack this transcription factor. The establishment of HIEC cells expressing Cdx2 in an inducible manner shows that forced expression of Cdx2 significantly alters the proliferation of intestinal crypt cells and stimulates dipeptidylpeptidase IV expression but is not sufficient to trigger intestinal terminal differentiation. These observations suggest that Cdx2 requires additional factors to activate the enterocyte differentiation program in normal undifferentiated cells.
Escaffit
Fabrice
F
CIHR Group on Functional Development and Physiopathology of the Digestive Tract, Département d'Anatomie et de Biologie Cellulaire, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, Que., Canada J1H 5N4.
Paré
Frédéric
F
Gauthier
Rémy
R
Rivard
Nathalie
N
Boudreau
François
F
Beaulieu
Jean-François
JF
D015415:Biomarkers; D000071616:CDX2 Transcription Factor; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology*; D009834:Oligo-1,6-Glucosidase / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D015415:Biomarkers; D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; D018398:Homeodomain Proteins; D013395:Sucrose; D009834:Oligo-1,6-Glucosidase; D018819:Dipeptidyl Peptidase 4