Multiplex analysis of expression of three IFNbeta-induced genes in antibody-positive MS patients.
Some interferon beta (IFNbeta)-treated patients with multiple sclerosis develop antibody-mediated decreased bioactivity with resultant loss of therapeutic effect. The authors developed real-time multiplex reverse transcriptase PCR to measure expression of three IFNbeta-inducible genes to directly assess IFNbeta bioactivity in patients with neutralizing antibodies (NAbs). The three genes responded in tandem. Correlation of NAb level with bioactivity at low/moderate NAb levels was poor, indicating that for such patients, direct measurement of IFNbeta bioactivity is most reliable.
University of Medicine and Dentistry of New Jersey, Medical School, Newark, NJ 07103, USA. pachner@umdnj.edu
Andrew R
AR
Pachner
Kavitha
K
Narayan
Elena
E
Pak
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics; D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D016022:Case-Control Studies; D019204:GTP-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D016899:Interferon-beta / Q000627:therapeutic use*; D009103:Multiple Sclerosis / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology*; D064547:Myxovirus Resistance Proteins; D009500:Neutralization Tests; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011506:Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000090123:Viperin Protein
D015088:2',5'-Oligoadenylate Synthetase; D000276:Adjuvants, Immunologic; D019204:GTP-Binding Proteins; D016899:Interferon-beta; D064547:Myxovirus Resistance Proteins; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011506:Proteins; C444427:RSAD2 protein, human; D000090123:Viperin Protein