Is there a possibility for a glutaraldehyde-free porcine heart valve to grow?
OBJECTIVE
A challenging issue is to create a heart valve with growth and remodeling potential, which would be of great interest for congenital heart valve surgery. This study was performed to evaluate the growth and remodeling potentials of a decellularized heart valve.


METHODS
In 4 juvenile sheep (age 12 +/- 1 weeks) with a weight of 24.3 +/- 4.4 kg, a 17-mm diameter decellularized porcine valve was implanted as pulmonary valve replacement. Valve growth was evaluated by transthoracic echocardiography. At explantation, valves were evaluated by gross examination, light microscopy (hematoxylin and eosin, von Kossa, Sirius red, Weigert and Gomori staining), electron microscopy and immunohistochemistry. Atomic absorption spectrometry was performed to evaluate calcium content.


RESULTS
All animals showed fast recovery. The mean follow-up was 9.0 +/- 1.8 months. All sheep at least doubled their weight (54.3 +/- 9.2 kg). Echocardiography showed no regurgitation and a flow velocity of 0.7 +/- 0.1 m/s at the latest follow-up. The valve diameter increased from 17.6 +/- 0.5 to 27.5 +/- 2.1 mm (p < 0.018). Gross examination showed a similar wall thickness of the implanted valve and native pulmonary wall, with smooth and pliable leaflets. Histology showed a monolayer of endothelial cells, fibroblast ingrowth and production of new collagen. No calcification was seen at von Kossa staining, confirmed by low calcium content levels of the valve wall and leaflets at atomic absorption spectrometry.


CONCLUSIONS
This glutaraldehyde-free heart valve showed not only the absence of calcification, but also remodeling and growth potential.
Department of Cardiovascular Surgery, Charité Hospital, Medical University, Berlin, Germany. pascal.dohmen@charite.de
P M
PM
Dohmen
F
F
da Costa
S
S
Holinski
S V
SV
Lopes
S
S
Yoshi
L H
LH
Reichert
R
R
Villani
S
S
Posner
W
W
Konertz
D000818:Animals; D017548:Echocardiography, Transesophageal; D005976:Glutaral / Q000494:pharmacology*; D006350:Heart Valve Prosthesis; D006351:Heart Valves / Q000187:drug effects / Q000254:growth & development*; D023421:Models, Animal; D011664:Pulmonary Valve / Q000254:growth & development* / Q000637:transplantation*; D012756:Sheep; D013552:Swine; D014183:Transplantation, Heterologous
D005976:Glutaral