[Clinical symptoms and mediators in the allergic early and late phase reaction].
BACKGROUND
This examination focused on the allergic early and late phase reaction via nasal symptom scores, acoustic rhinometry, and the determination of mediators possibly involved in late phase eosinophilia. We examined nasal secretions for IL-5; the chemokines IL-8, MCP-1, and Eotaxin; the adhesion molecule sVCAM-1, and the leukotriene LTC4 for their suggested impacts on tissue eosinophilia.


METHODS
13 patients suffering from seasonal allergic rhinitis were challenged intranasally out of the natural pollen season by their specific allergen. In a time window of 8 h following the provocation, patients completed symptom questionnaires, and underwent acoustic rhinometry. Nasal secretions were gained by the cotton wool method over a time period of 8 h. Nasal secretions were analyzed for the above mentioned mediators.


RESULTS
Individual evaluation of the acoustic rhinometry measurements revealed an early phase reaction in 100 % of the cases and a late phase reaction in 92 %. The need to sneeze and a runny nose were the strongest symptoms during the allergic early and late phase reaction. A typical late phase kinetic was observed for IL-5, MCP-1, Eotaxin, sVCAM-1, and LTC4. IL-8 was characteristic for early phase reaction but increased in late phase as well.


CONCLUSIONS
The need to sneeze, a runny nose, and the overall quality of life were most apt to evaluate the allergic early and late phase reaction. Highly significant correlations between nasal obstruction and acoustic rhinometry measurements indicate a high sensitivity of visual analogue scales in the representation of minimal changes in nasal symptom scores during the allergic reaction. Our data point to a relevant role of the TH2 cytokine IL-5; of the chemokines IL-8, MCP-1, and Eotaxin; of the adhesion molecule sVCAM-1, and of the leukotriene LTC4 for the allergic late phase eosinophilia.
Klinik und Poliklinik für Hals-, Nasen- und Ohrenheilkunde am Klinikum Grosshadern, Ludwig-Maximilians-Universität München.
T R
TR
Jordan
E
E
Pfrogner
G
G
Rasp
M F
MF
Kramer
D000328:Adult; D054413:Chemokine CCL11; D019742:Chemokines, CC / Q000378:metabolism; D004802:Eosinophilia / Q000209:etiology; D005260:Female; D006801:Humans; D015848:Interleukin-5 / Q000378:metabolism; D016906:Interleukin-9 / Q000378:metabolism; D017997:Leukotriene C4 / Q000378:metabolism; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism; D009299:Nasal Provocation Tests; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis* / Q000276:immunology / Q000503:physiopathology; D023842:Rhinometry, Acoustic; D011795:Surveys and Questionnaires; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis
C515456:CCL11 protein, human; D054413:Chemokine CCL11; D019742:Chemokines, CC; D015848:Interleukin-5; D016906:Interleukin-9; D019010:Vascular Cell Adhesion Molecule-1; D017997:Leukotriene C4