Yeast two-hybrid detection systems that are highly sensitive to a certain kind of endocrine disruptors.
We tested the effects of several combinations of bait and fish components of the yeast two-hybrid detection system for estrogenic activity. A combination of the full-length human estrogen receptor alpha with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1) or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen-dependent induction of the chromosome-integrated UAS(GAL)-CYC1(p)-lacZ reporter construct among the two-hybrid systems so far tested.
Department of Biotechnology, University of Tokyo, Japan.
Haeng-Seog
HS
Lee
Shin-ichi
S
Sasagawa
Shigeaki
S
Kato
Ryouichi
R
Fukuda
Hiroyuki
H
Horiuchi
Akinori
A
Ohta
D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology*; D017930:Genes, Reporter / Q000235:genetics; D051548:Histone Acetyltransferases; D006801:Humans; D056920:Nuclear Receptor Coactivator 1; D051958:Nuclear Receptor Coactivator 2 / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D012680:Sensitivity and Specificity; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D047628:Estrogen Receptor alpha; D004967:Estrogens; D051958:Nuclear Receptor Coactivator 2; D014157:Transcription Factors; D051548:Histone Acetyltransferases; C538859:NCOA1 protein, human; D056920:Nuclear Receptor Coactivator 1