Erythropoietin-induced proliferation of gastric mucosal cells.
OBJECTIVE
To analyze the localization of erythropoietin receptor on gastric specimens and characterize the effects of erythropoietin on the normal gastric epithelial proliferation using a porcine gastric epithelial cell culture model.


METHODS
Erythropoietin receptor was detected by RT-PCR, Western blotting and immunohistochermistry. Growth stimulation effects of erythropoietin on cultured gastric mucosal cells were determined by ELISA using bromodeoxyuridine (BrdU).


RESULTS
Erythropoietin receptor was detected on cultured porcine gastric mucosal epithelial cells. Erythropoietin receptor was also detected histochemically at the base of gastric mucosal epithelium. BrdU assay demonstrated a dose-dependent increase in growth potential of cultured porcine gastric mucosal epithelial cells by administration of erythropoietin, as well as these effects were inhibited by administration of anti- erythropoietin antibody (P<0.01).


CONCLUSIONS
These findings indicate that erythropoietin has a potential to proliferate gastric mucosal epithelium via erythropoietin receptor.
Second Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa City, Saitama 359-8513, Japan. kazuito@ndmc.ac.jp
Kazuro
K
Itoh
Yoshio
Y
Sawasaki
Kyoko
K
Takeuchi
Shingo
S
Kato
Nobuhiro
N
Imai
Yoichiro
Y
Kato
Noriyuki
N
Shibata
Makio
M
Kobayashi
Yoshiyuki
Y
Moriguchi
Masato
M
Higuchi
Fumio
F
Ishihata
Yushi
Y
Sudoh
Soichiro
S
Miura
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004921:Erythropoietin / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D007150:Immunohistochemistry; D017467:Receptors, Erythropoietin / Q000032:analysis* / Q000235:genetics; D013552:Swine
D017467:Receptors, Erythropoietin; D004921:Erythropoietin