Comparative proteome approach to characterize the high-pressure stress response of Lactobacillus sanfranciscensis DSM 20451(T).
High hydrostatic pressure (HHP) exerts diverse effects on microorganisms, leading to stress response and cell death. While inactivation of microorganisms by lethal HHP is well investigated in the context of food preservation and the hygienic safety of minimal food processes, sublethal HHP stress response and its effect on adaptation and cross-protection is less understood. In this study, the HHP stress response of Lactobacillus sanfranciscensis was characterized and compared with cold, heat, salt, acid and starvation stress at the proteome level by using 2-DE so as to provide insight into general versus specific stress responses. Sixteen proteins were found to be affected by HHP and were identified by using N-terminal amino acid sequencing and MS. Only one slightly increased protein was specific to the HHP response and showed homology to a clp protease. The other proteins were influenced by most of the investigated stresses in a similar way as HHP. The highest similarity in the HHP proteome was found to be with cold- and NaCl-stressed cells, with 11 overlapping proteins. At the proteome level, L. sanfranciscensis appears to use overlapping subsets of stress-inducible proteins rather than stereotype responses. Our data suggest that a specific pressure response does not exist in this bacteria.
Hörmann
Sebastian
S
Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Freising-Weihenstephan, Germany.
Scheyhing
Carsten
C
Behr
Jürgen
J
Pavlovic
Melanie
M
Ehrmann
Matthias
M
Vogel
Rudi F
RF
D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D018869:Heat-Shock Response; D006874:Hydrostatic Pressure; D007091:Image Processing, Computer-Assisted; D007778:Lactobacillus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D020539:Sequence Analysis, Protein; D016622:Silver Staining; D012965:Sodium Chloride / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology
D020543:Proteome; D012965:Sodium Chloride; D014357:Trypsin