L-serine and GABA uptake by synaptosomes during postnatal development of rat.
Postnatal development changes in mechanisms of synaptosomal amino acid transport have been studied in rat cerebral cortex. Specific uptake of radiolabeled L-serine was examined and compared with that of radiolabeled GABA using synaptosomes-enriched fractions freshly prepared from cerebral cortex at different postnatal days from the birth to young adulthood. The preparations were incubated with 10 nM of [3H]L-serine and 10 nM of [3H]-GABA in either the presence or absence of NaCl, KCl or choline chloride, at 2 and 30 degrees C, for different periods up to 30 min. The uptake of [3H]l-serine was temperature dependent in synaptosomal fractions prepared from cerebral cortex of rats in postnatal days 5, 7, 13 and 21, but stronger dependence was observed in adult brain, irrespective of the presence of Na+, K+ or choline ions. At all postnatal ages studied, [3H]-GABA uptake showed a high activity in the presence of Na+ ions and at 30 degrees C. The values of Km were 90-489 microM in L-serine uptake. However, in the uptake of GABA the values of Km were 80-150 microM. The highest values of Vmax were obtained at 5 and 21 postnatal days for both transport systems. These results indicate that the uptake of l-serine and GABA are regulated differentially during postnatal development.
Cheluja
María Gabriela
MG
Instituto de Investigaciones Farmacológicas (ININFA-CONICET-UBA), Junín 956. 5 piso, C1113AAD, Buenos Aires, Argentina.
Scolari
Mariano José
MJ
Coelho
Trinidad María
TM
Blake
Mariano Guillermo
MG
Boccia
Mariano Martín
MM
Baratti
Carlos María
CM
Acosta
Gabriela Beatriz
GB
D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport; D002540:Cerebral Cortex / Q000254:growth & development*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012694:Serine / Q000378:metabolism* / Q000493:pharmacokinetics; D013574:Synaptosomes / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000493:pharmacokinetics
D012694:Serine; D005680:gamma-Aminobutyric Acid