DAZ1/DAZ2 cluster deletion mediated by gr/gr recombination per se may not be sufficient for spermatogenesis impairment: a study of Chinese normozoospermic men.
OBJECTIVE
To explore the possible effect of the deleted in azoospermia (DAZ) copy cluster deletion on spermatogenesis in the Chinese population, the deletion of the azoospermia factor c (AZFc) region was analyzed in 346 normozoospermic men.


METHODS
Three DAZ single nucleotide variant loci and seven AZFc-specific sequence-tagged sites were examined with polymerase chain reaction (PCR)-restriction fragment length polymorphism and routine PCR.


RESULTS
Five (1.4%) of the normozoospermic men were found to have deletion of gr/gr-DAZ1/DAZ2. None of the men were found to have b2/b4-entire DAZ deletion.


CONCLUSIONS
The presence of gr/gr-DAZ1/DAZ2 deletion in five men with normozoospermia suggests that this deletion per se may not be sufficient for spermatogenic impairment in Chinese men.
Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu 610041, China.
Yuan
Y
Yang
Cui-Ying
CY
Xiao
Zhou-Cun
ZC
A
Si-Zhong
SZ
Zhang
Xiang
X
Li
Si-Xiao
SX
Zhang
D000328:Adult; D044466:Asian People / Q000235:genetics; D002681:China; D041322:Chromosomes, Human, Y / Q000235:genetics*; D017353:Gene Deletion; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D016601:RNA-Binding Proteins / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D016324:Sequence Tagged Sites; D013091:Spermatogenesis / Q000235:genetics*
C412481:DAZAP1 protein, human; C498224:DAZAP2 protein, human; D016601:RNA-Binding Proteins