Surfactant protein D in atopic dermatitis and psoriasis.
The collectin surfactant protein-D (SP-D) shows antimicrobial and immuno-regulatory properties and has recently been detected in the basal layers of normal human skin. This molecule potentially plays an important role in inflammatory skin diseases and therefore SP-D content and location was examined using immunohistochemistry on skin biopsies from patients with the two major dermatologic diseases, psoriasis and atopic dermatitis. SP-D was located in the stratum basale of all biopsies with similar intense staining in both diseased and normal skin. Differences were detected in stratum spinosum where involved psoriatic skin showed intense staining through the entire region significantly different from uninvolved and normal skin. Lesional atopic skin showed moderate staining extending through the basal three-fourths of stratum spinosum. Using real time polymerase chain reaction analysis, no substantial up-regulation of SP-D mRNA was detected in lesional psoriatic skin, and a comparison of serum levels of SP-D between patients with atopic dermatitis or psoriasis and a group of age matched healthy controls did not show significant differences. In conclusion SP-D was significantly more abundant in the stratum spinosum of lesional psoriatic and atopic skin due to more cells producing the molecule rather than up-regulation of production in single cells of diseased skin. Further studies are needed to show if SP-D plays a role in the protection against skin infections or modulation of the inflammatory process in these common skin diseases.
Department of Dermatology and Venerology, Aarhus University Hospital, P.P. Ørumsgade 11, DK-8000 Aarhus C, Denmark. thomashohwy@hotmail.com
Thomas
T
Hohwy
Kristian
K
Otkjaer
Jens
J
Madsen
Gritt
G
Søerensen
Ole
O
Nielsen
Christian
C
Vestergaard
Torben
T
Steiniche
Uffe
U
Holmskov
Hans
H
Lomholt
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003876:Dermatitis, Atopic / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011565:Psoriasis / Q000378:metabolism*; D037663:Pulmonary Surfactant-Associated Protein D / Q000378:metabolism*
D037663:Pulmonary Surfactant-Associated Protein D