[Genetically modified industrial brewing yeast with high-glutathione and low-diacetyl production].
Recombinant plasmid pICG was constructed by replacing the internal fragment of a-acetohydroxyacid synthase (AHAS) gene (ILV2) with a copy of gamma-glutamylcysteine synthetase gene (GSH1) and copper chelatin gene (CUP1) from the industrial brewing yeast strain YSF31. YSF31 was transformed with plasmid pICG linearized by Kpn I and Pst I. A recombinant strain with high-glutathione and low-diacetyl production was selected. The results of fermentation in 100-L bioreactor showed that the lagering time of beer produced for recombinant strain T2 was shortened by 3 days and the shelf life of the beer was prolonged about 50%. It may be more acceptable for the commercial application, as it does not contain foreign DNA.
Zhang
Ji-Na
JN
The Laboratory of Molecular Genetics and Breeding of Yeast, Institute of Microbiology of Chinese Academy of Sciences, Beijing, 100080, China.
He
Xiu-Ping
XP
Guo
Xue-Na
XN
Liu
Nan
N
Zhang
Bo-Run
BR
D000095:Acetolactate Synthase / Q000235:genetics / Q000378:metabolism; D001515:Beer / Q000382:microbiology*; D003001:Cloning, Molecular; D003931:Diacetyl / Q000378:metabolism*; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis*; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism; D030781:Organisms, Genetically Modified / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
C500769:CUP1-1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D008668:Metallothionein; D000095:Acetolactate Synthase; C490862:GSH1 protein, S cerevisiae; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione; D003931:Diacetyl