[TNF-alpha gene expression of NAFLD rat intervened by the extracts of Rizoma Polygoni Cuspidati].
The real-time qPCR method had been used to detect and analyze the non-alcohol fatty liver disease (NEFLD) model in medical intervention in this research. The relative level of TNF-alpha mRNA in adipose tissue of intervention group was lower than that of control group. Their difference was significant (t = 2.452, P = 0.22). Compared with the control group, it decreased that the contents of liver trilyceride, total cholesterol, and glucose in intervention group. The difference of total cholesterol between two groups was significant (t = 2.555, P = 0.019). The extracts of Rizoma Polygoni Cuspidati could significantly decrease TNF-alpha mRNA level in adipose tissue, and it could decrease the contents of triglyceride, total cholesterol, and glucose in liver tissue. This Chinese traditional medicine can adjust the metabolism of liver adipose and glucose,and improve steatosis in liver cell.
Jiang
Qinglan
Q
Core Lab, The First Municipal People's Hospital of Guangzhou, Guangzhou 510180.
Li
Yuyuan
Y
Pan
Jinyao
J
Ma
Jun
J
Xu
Banglao
B
Yang
Hui
H
Zhang
Junli
J
He
Miao
M
D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D045468:Fallopia japonica / Q000737:chemistry*; D005234:Fatty Liver / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D015870:Gene Expression / Q000187:drug effects; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014280:Triglycerides / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D002784:Cholesterol