Rewritable DNA microarrays.
Thiol-terminated single-stranded deoxyribonucleic acids (ssDNA) can be immobilized onto pulsed plasma deposited poly(allylmercaptan) surfaces via disulfide bridge chemistry and are found to readily undergo nucleic acid hybridization. Unlike other methods for oligonucleotide attachment to solid surfaces, this approach is shown to be independent of substrate material or geometry, and amenable to highly efficient rewriting.
Department of Chemistry, Science Laboratories, and School of Biological and Biomedical Science, Durham University, Durham DH1 3LE, England, UK.
W C E
WC
Schofield
J
J
McGettrick
T J
TJ
Bradley
J P S
JP
Badyal
S
S
Przyborski
D004277:DNA, Single-Stranded / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013438:Sulfhydryl Compounds / Q000737:chemistry
D004277:DNA, Single-Stranded; D004220:Disulfides; D015345:Oligonucleotide Probes; D013438:Sulfhydryl Compounds