Encapsulation of endoglucanase using a biopolymer Gum Arabic for its controlled release.
Gum Arabic, a biodegradable natural polymer was used as a matrix to encapsulate endoglucanase from Thermomonospora sp. The modified enzyme retained complete biocatalytic activity and exhibited a shift in the optimum temperature [50-55 degrees C] and considerable increase in the pH and temperature stabilities as compared to the free enzyme. Encapsulation of the enzyme also protected the activity in presence of detergents and enhanced the shelf life. A 3-fold decrease in the initial rate of reaction indicated a controlled release of the enzyme conferring properties preferred for its potential application in the manufacture of detergents.
Biochemical Sciences Division, National Chemical Laboratory, Pune, Maharashtra 411 008, India.
Anish
A
Ramakrishnan
Nilesh
N
Pandit
Mahesh
M
Badgujar
C
C
Bhaskar
Mala
M
Rao
D001704:Biopolymers / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism*; D006170:Gum Arabic / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D013997:Time Factors
D001704:Biopolymers; D006170:Gum Arabic; D002480:Cellulase