[Study of single nucleotide polymorphism of A-FABP gene and its association with fatness traits in chicken].
In this experiment, Beijing-You chicken was used to detect single nucleotide polymorphisms (SNPs) in A-FBAP gene and to study the correlation between its genotype and the trait of fat accumulation. The first exon of the gene was amplified by one pair of primers, and SNPs were detected by the technique of single strand conformation polymorphism (SSCP) and finally confirmed by sequencing. Results of analysis of variance showed that a significant difference existed among abdominal fat percentage, subcutaneous fat thickness and intramuscular fat contents in breast musculature with different genotypes (P<0.01). It implied that A-FABP gene could be a major effector gene or could be linked to gene(s) which significantly affect fat metabolism in chicken.
Institute Animal Science, CAAS , Beijing 100094, China. lgf5156@sina.com
Gui-Fen
GF
Luo
Ji-Lan
JL
Chen
Jie
J
Wen
Gui-Ping
GP
Zhao
Mai-Qing
MQ
Zheng
Shi-Duo
SD
Sun
D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D001823:Body Composition / Q000235:genetics*; D002645:Chickens; D005091:Exons / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005838:Genotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational
D050556:Fatty Acid-Binding Proteins