[An experimental research of hyperthermic preconditioning on the spinal cord injury].
OBJECTIVE
To assess the effects of hyperthermic preconditioning on spinal cord injury(SCI) in rats.


METHODS
A total of 96 SD rats were divided into control(A), trauma(B) and hyperthermic preconditioning (C) group. SCI models were made by static compression. The animals were decapitated at 6 h, 12 h, 1 d, 2 d, 3 d, 1 w, 2 w and 3 w after injury. With in situ hybridization and immunohistochemistry, the expression of HSP70 after spinal cord injury was studied at the transcript level and translation level respectively. Neurological outcome was evaluated by the combined behavioral score (CBS).


RESULTS
Neurological outcome in Group C was significantly higher than that in Group B (P < 0.05). In-situ hybridization and Immunohistochemistry analysis showed that expression of HSP70 in spinal cord elevated after hyperthermic preconditioning; furthermore, the level of HSP70 in Group C was significantly higher than that in Group B (P < 0.05).


CONCLUSIONS
Hyperthermic preconditioning might improve neurological outcome after spinal cord injury in rats, and the protective mechanism in this connection might involve the induction of HSP70 synthesis in spinal cord.
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Yong
Y
Wang
Bo-yong
BY
Mao
Yi-ling
YL
Deng
Dan
D
Xiao
D000818:Animals; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006979:Hyperthermia, Induced / Q000379:methods*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology; D013119:Spinal Cord Injuries / Q000628:therapy*; D013997:Time Factors
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger