Anal electrical stimulation with long pulses increases anal sphincter pressure in conscious dogs.
OBJECTIVE
This study was designed to investigate the effects and mechanisms of anal electric stimulation with long pulses on anal sphincter pressure in conscious dogs.


METHODS
The study was performed after enema in nine healthy female hound dogs and composed of four randomized sessions ("dose"-response, anal electric stimulation only, or with atropine or phentolamine). The anal sphincter pressure was measured by using manometry and quantified by using the area under the contractile curve (mmHg/sec). Anal electric stimulation was performed via a pair of ring electrodes attached to a manometric catheter. The stimulation parameters in all but dose-response sessions included a frequency of 20 ppm, pulse width of 200 ms, and amplitude of 3 mA.


RESULTS
The anal sphincter pressure was 55.7 +/- 6 at baseline and increased by 37 percent to 76.4 +/- 6.5 during electric stimulation (P = 0.009). The increase of anal pressure during stimulation was positively correlated with the stimulation energy (r = 0.395; P < 0.01). The excitatory effect of electric stimulation was sustained for at least 20 minutes. Atropine did not alter anal pressure and did not abolish the excitatory effect of anal electric stimulation on the sphincter. Phentolamine reduced anal pressure from the baseline value of 50.5 +/- 4.7 to 33.1 +/- 5.4 (P = 0.019). The electric stimulation induced increase in anal pressure was dropped from 19 +/- 2.6 to 9.9 +/- 2.8 (P = 0.029) at the presence of phentolamine.


CONCLUSIONS
Anal electric stimulation with long pulses increases anal sphincter pressure in an energy-dependent manner. The alpha-adrenergic but not the cholinergic pathway at least partially mediates the excitatory effect of anal electric stimulation.
Division of Gastroenterology, University of Texas Medical Branch, Galveston, Texas 77555-0632, USA.
Yuqiang
Y
Nie
Jay P
JP
Pasricha
Jiande D Z
JD
Chen
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D001003:Anal Canal / Q000502:physiology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004285:Dogs; D004558:Electric Stimulation / Q000379:methods*; D005260:Female; D008365:Manometry; D018727:Muscarinic Antagonists / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D011312:Pressure
D000317:Adrenergic alpha-Antagonists; D018727:Muscarinic Antagonists; D001285:Atropine; D010646:Phentolamine