Putative neutralization epitopes and broad cross-genotype neutralization of Hepatitis E virus confirmed by a quantitative cell-culture assay.
Monolayers of Hep G2/C3A cells were inoculated with genotype 1 Hepatitis E virus (HEV) mixed with either anti-HEV or an appropriate control. After 5 or 6 days, cell monolayers were stained with anti-HEV and infected cells were identified by immunofluorescence microscopy and counted. Anti-HEV from vaccinated or infected rhesus monkeys neutralized the virus, as did mAbs that recognized epitopes on the C terminus of a recombinant vaccine protein. Antibodies were broadly cross-reactive, since convalescent serum from animals infected with any one of the four mammalian genotypes all neutralized the genotype 1 virus.
Molecular Hepatitis and Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 50 South Drive MSC-8009, Bethesda, MD 20892-8009, USA.
Suzanne U
SU
Emerson
Molecular Hepatitis and Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 50 South Drive MSC-8009, Bethesda, MD 20892-8009, USA.
Pilar
P
Clemente-Casares
Molecular Hepatitis and Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 50 South Drive MSC-8009, Bethesda, MD 20892-8009, USA.
Nasser
N
Moiduddin
Hepatitis Division, National Institute of Virology, Pune, India.
Vidya A
VA
Arankalle
Molecular Hepatitis and Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 50 South Drive MSC-8009, Bethesda, MD 20892-8009, USA.
Udana
U
Torian
Molecular Hepatitis and Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 50 South Drive MSC-8009, Bethesda, MD 20892-8009, USA.
Robert H
RH
Purcell
D000818:Animals; D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood / Q000276:immunology*; D016751:Hepatitis E / Q000097:blood / Q000821:virology*; D016752:Hepatitis E virus / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008253:Macaca mulatta; D009500:Neutralization Tests / Q000379:methods; D013045:Species Specificity; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology; D014761:Viral Hepatitis Vaccines / Q000276:immunology
D000939:Epitopes; D006508:Hepatitis Antibodies; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D014614:Vaccines, Synthetic; D014761:Viral Hepatitis Vaccines