GEF and glucosylation assays on liposome-bound Rac.
Rac binds tightly to lipid membranes through a lipid modification. The influence of the lipid membrane environment on the multiple interactions of Rac has not been well documented. In this chapter, we detail a method to prepare geranyl-geranylated Rac bound to liposomes of defined composition. With this method, one can dissect some lipid-protein interactions that facilitate the interaction of Rac with other proteins such as guanine nucleotide exchange factors and bacterial toxins.
Mesmin
Bruno
B
CNRS-Institut de Pharmacologie, Moleculaire et Cellulaire, Valbonne, France.
Antonny
Bruno
B
D005964:Glucosyltransferases / Q000378:metabolism*; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D010713:Phosphatidylcholines; D010743:Phospholipids; D020744:rac GTP-Binding Proteins / Q000378:metabolism*
D020730:Guanine Nucleotide Dissociation Inhibitors; D020662:Guanine Nucleotide Exchange Factors; D008081:Liposomes; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D006153:Guanosine Diphosphate; C024990:asolectin; D005964:Glucosyltransferases; D020744:rac GTP-Binding Proteins