Tetratricopeptide repeats are essential for PcrH chaperone function in Pseudomonas aeruginosa type III secretion.
The type III secretion system (T3SS) is a specialized apparatus evolved by Gram-negative bacteria to deliver effector proteins into host cells, thus facilitating the establishment of an infection. Effector translocation across the target cell plasma membrane is believed to occur via pores formed by at least two secreted translocator proteins, the functions of which are dependent upon customized class II T3SS chaperones. Recently, three internal tetratricopeptide repeats (TPRs) were identified in this class of chaperones. Here, defined mutagenesis of the class II chaperone PcrH of Pseudomonas aeruginosa revealed these TPRs to be essential for chaperone activity towards the translocator proteins PopB and PopD and subsequently for the translocation of exoenzymes into host cells.
Bröms
Jeanette E
JE
Department of Molecular Biology, Umeå University, Umeå, Sweden.
Edqvist
Petra J
PJ
Forsberg
Ake
A
Francis
Matthew S
MS
D036002:ADP Ribose Transferases / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism* / Q000502:physiology*; D001427:Bacterial Toxins; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000379:methods; D010641:Phenotype; D052899:Pore Forming Cytotoxic Proteins / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000502:physiology*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013997:Time Factors; D020798:Two-Hybrid System Techniques
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D018832:Molecular Chaperones; C412007:PopB protein, Ralstonia solanacearum; D052899:Pore Forming Cytotoxic Proteins; D036002:ADP Ribose Transferases; C444820:exoenzyme S