3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase is regulated for the accumulation of polysaccharide-linked hydroxycinnamoyl esters in rice (Oryza sativa L.) internode cell walls.
Polysaccharide-linked hydroxycinnamoyl esters (PHEs) over-accumulate in the internodes of a rice (Oryza sativa L.) mutant, Fukei 71 (F71). This accumulation is accompanied by over-expression of phenylalanine ammonialyase (PAL). In this study, we show that only one member of the 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAHPS) family expresses in close correlation with PAL. Furthermore, substrate availability to DAHPS is promoted by down-regulating the expression of plastidic pyruvate kinase (PKp), a competitor of DAHPS. Since the over-production of PHEs is caused by D50 gene disruption, these results suggest that specific enzymes in the phenylpropanoid and shikimate pathways are coordinately up-regulated. In addition, the results indicate that carbon-flow into the shikimate pathway is modified for the synthesis of PHEs, and is probably controlled by D50.
Sato
Kanna
K
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, 184-8588, Japan.
Mase
Kohei
K
Nakano
Yoshimi
Y
Nishikubo
Nobuyuki
N
Sugita
Rika
R
Tsuboi
Yuuri
Y
Kajita
Shinya
S
Zhou
Jinmei
J
Kitano
Hidemi
H
Katayama
Yoshihiro
Y
D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D002473:Cell Wall / Q000201:enzymology / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism*; D004952:Esters / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism*
D003373:Coumaric Acids; D004952:Esters; D011134:Polysaccharides; D010723:3-Deoxy-7-Phosphoheptulonate Synthase