[Preliminary identification and characterization of in vitro cultured bone marrow stromal cells for their bioactivity and osteogenic potential].
OBJECTIVE
To induce the differentiation of bone marrow stromall cells (BMSCs) isolated from Beagles into osteoblasts in vitro and identify the osteogenic potential and bioactivity of the BMSCs.


METHODS
Primary cultured BMSCs isolated from Beagles were subcultured in mineralization medium to induce their differentiation into osteoblasts, whose morphological characteristics and proliferation status were observed by phase-contrast microscope. The osteogenic activity of the cells was evaluated with von Kossa staining of the mineralized nodules and determination of the alkaline phosphatase activity.


RESULTS
BMSCs cultured in vitro showed obvious osteogenic capacity in DMEM. Von Kossa staining of the mineralized nodules and alkaline phosphatase detection of the passaged cells both yielded positive results.


CONCLUSIONS
BMSCs cultured in vitro contain osteogenic precursor cells, and the passaged cells possess osteogenic potential.
Department of Stomatology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. renliwn@126.com
Xiao-li
XL
Tu
Hong-wei
HW
Liu
Cai-liang
CL
Li
Xiao-hua
XH
Ren
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004285:Dogs; D008297:Male; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D017154:Stromal Cells / Q000166:cytology