RNA specific molecules: cytotoxic plant alkaloid palmatine binds strongly to poly(A).
The cytotoxic plant alkaloid palmatine was found to bind strongly by partial intercalation to single stranded poly(A) structure with binding affinity (Ka) of (8.36+/-0.26) x 10(5) M(-1). The binding of palmatine was characterized to be exothermic and enthalpy driven with one palmatine for every two adenine residues. On the other hand, the binding to the double stranded poly(A) has been found to be significantly weak. This study identifies poly(A) as a potential bio-target for the alkaloid palmatine and its use as a lead compound in antitumor drug screening.
Biophysical Chemistry Laboratory, Indian Institute of Chemical Biology, Kolkata 700 032, India.
Prabal
P
Giri
Maidul
M
Hossain
Gopinatha Suresh
GS
Kumar
D000970:Antineoplastic Agents / Q000737:chemistry*; D001600:Berberine Alkaloids / Q000737:chemistry*; D002942:Circular Dichroism; D004353:Drug Evaluation, Preclinical; D006860:Hydrogen Bonding; D015394:Molecular Structure; D011061:Poly A / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013816:Thermodynamics
D000970:Antineoplastic Agents; D001600:Berberine Alkaloids; D011061:Poly A; D012313:RNA; C005413:palmatine