All4312, an NtcA-regulated two-component response regulator in Anabaena sp. strain PCC 7120.
All4312, encoded by open reading frame all4312 in the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, exhibits a CheY-like receiver domain and an output domain similar to that of OmpR, characteristic of two-component response regulators. Expression of all4312 was directly regulated by NtcA, the global transcriptional regulator of nitrogen assimilation in cyanobacteria. Features characteristic of NtcA-activated promoters were also found upstream from genes encoding All4312 homologues in several other cyanobacterial genomes. Expression of all4312 was however unaffected in a mutant of hetR, which encodes a regulator triggering heterocyst development. The function of All4312 may be related to the cellular response to nitrogen deprivation.
Muro-Pastor
Alicia María
AM
Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Seville, Spain. alicia@ibvf.csic.es
Olmedo-Verd
Elvira
E
Flores
Enrique
E
D000643:Ammonium Chloride / Q000494:pharmacology; D017033:Anabaena / Q000254:growth & development / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D015152:Blotting, Northern / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D009154:Mutation / Q000502:physiology; D009584:Nitrogen / Q000494:pharmacology; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D015534:Trans-Activators / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; D003470:Culture Media; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C112177:osmolarity response regulator proteins; D000643:Ammonium Chloride; C067403:HetR protein, Bacteria; D049452:Green Fluorescent Proteins; C075617:ntcA protein, Synechococcus; D009584:Nitrogen