Human placental protein/peptides stimulate melanin synthesis by enhancing tyrosinase gene expression.
Placental protein/peptides as biological response modifier are well documented, but not much known about melanogenesis. We possibly for the first time, demonstrated melanogenesis in B16F10 mouse melanoma by a placental protein/peptide fraction (PPPF) prepared from a hydroalcoholic extract of fresh term human placenta. This study described the effect of PPPF on the induction of tyrosinase; the key enzyme of melanogenesis to investigate the basis of PPPF induced pigmentation in primary melanocyte and B16F10 melanoma. Tyrosinase induction by PPPF in B16F10 cells was found dose- and time dependent at the level of activity. Tyrosinase, at the level of transcription and protein expression when assessed by RT-PCR and Western blot analyses found to have considerable induction over untreated control. PPPF led to enhanced activation of tyrosinase promoter resulting higher transcription thus substantiating the role of PPPF as a stimulator of melanogenesis. Actinomycin D, the transcriptional inhibitor of protein synthesis, blocked the stimulatory action of PPPF since the induction of tyrosinase and melanin was markedly reduced in presence of this inhibitor. Thus the results suggested that PPPF mediated increase in tyrosinase expression occurred through transcriptional upregulation to stimulate melanogenesis in B16F10 cells and in primary melanocyte also.
Sarkar
Chinmoy
C
Department of Cellular Biochemistry, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, 700032, Jadavpur, Kolkata, India.
Singh
Suman Kumar
SK
Mandal
Samir Kumar
SK
Saha
Bidisha
B
Bera
Rabindranath
R
Ratha
Jagnyeswar
J
Datta
Pijush Kanti
PK
Bhadra
Ranjan
R
D000818:Animals; D018929:Cell Culture Techniques; D002851:Chromatography, High Pressure Liquid; D003609:Dactinomycin / Q000494:pharmacology; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics*; D011257:Pregnancy Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000235:genetics
D008543:Melanins; D011257:Pregnancy Proteins; D003609:Dactinomycin; D014442:Monophenol Monooxygenase