Efficient cleavage by signal peptide peptidase requires residues within the signal peptide between the core and E1 proteins of hepatitis C virus strain J1.
Maturation of hepatitis C virus (HCV) core protein requires cleavage by signal peptidase (SP) and signal peptide peptidase (SPP) at a signal peptide between core and the E1 glycoprotein. For HCV strain Glasgow, amino acids Ala(180), Ser(183) and Cys(184) within the signal peptide have previously been shown to be essential for efficient SPP cleavage. By contrast, these residues apparently did not contribute to core maturation in HCV strain J1. In the present study, the source of this discrepancy has been analysed and it is concluded that interpretation of the strain J1 data was incorrect, due to the inability to separate wild-type and mutant forms of core on gels by using standard buffer systems.
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
R Graham
RG
Hope
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
Marion J
MJ
McElwee
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
John
J
McLauchlan
D000595:Amino Acid Sequence; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism*; D002460:Cell Line; D016174:Hepacivirus / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology*; D014758:Viral Core Proteins / Q000096:biosynthesis* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000096:biosynthesis*
C087035:E1 protein, Hepatitis C virus; D021382:Protein Sorting Signals; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; C069130:nucleocapsid protein, Hepatitis C virus; D016282:Aspartic Acid Endopeptidases; C460085:signal peptide peptidase