Effects of water restriction on gene expression in mouse renal medulla: identification of 3betaHSD4 as a collecting duct protein.
To identify novel gene targets of vasopressin regulation in the renal medulla, we performed a cDNA microarray study on the inner medullary tissue of mice following a 48-h water restriction protocol. In this study, 4,625 genes of the possible approximately 12,000 genes on the array were included in the analysis, and of these 157 transcripts were increased and 63 transcripts were decreased by 1.5-fold or more. Quantitative, real-time PCR measurements confirmed the increases seen for 12 selected transcripts, and the decreases were confirmed for 7 transcripts. In addition, we measured transcript abundance for many renal collecting duct proteins that were not represented on the array; aquaporin-2 (AQP2), AQP3, Pax-8, and alpha- and beta-Na-K-ATPase subunits were all significantly increased in abundance; the beta- and gamma-subunits of ENaC and the vasopressin type 1A receptor were significantly decreased. To correlate changes in mRNA expression with changes in protein expression, we carried out quantitative immunoblotting. For most of the genes examined, changes in mRNA abundances were not associated with concomitant protein abundance changes; however, AQP2 transcript abundance and protein abundance did correlate. Surprisingly, aldolase B transcript abundance was increased but protein abundance was decreased following 48 h of water restriction. Several transcripts identified by microarray were novel with respect to their expression in mouse renal medullary tissues. The steroid hormone enzyme 3beta-hydroxysteroid dehydrogenase 4 (3betaHSD4) was identified as a novel target of vasopressin regulation, and via dual labeling immunofluorescence we colocalized the expression of this protein to AQP2-expressing collecting ducts of the kidney. These studies have identified several transcripts whose abundances are regulated in mouse inner medulla in response to an increase in endogenous vasopressin levels and could play roles in the regulation of salt and water excretion.
Department of Physiology, College of Medicine, 1501 N Campbell Ave, University of Arizona, Tucson, AZ 85724-5051, USA.
Qi
Q
Cai
Maggie
M
Keck
Matthew R
MR
McReynolds
Janet D
JD
Klein
Kevin
K
Greer
Kumar
K
Sharma
James B
JB
Hoying
Jeff M
JM
Sands
Heddwen L
HL
Brooks
D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis* / Q000235:genetics*; D000818:Animals; D051399:Aquaporin 2 / Q000032:analysis / Q000235:genetics / Q000502:physiology; D051400:Aquaporin 3 / Q000032:analysis / Q000235:genetics / Q000502:physiology; D018076:DNA, Complementary / Q000032:analysis; D053503:Epithelial Sodium Channels; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis / Q000235:genetics / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007679:Kidney Medulla / Q000737:chemistry* / Q000502:physiology; D007685:Kidney Tubules, Collecting / Q000737:chemistry* / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors / Q000032:analysis / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D017483:Receptors, Vasopressin / Q000032:analysis / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015222:Sodium Channels / Q000032:analysis / Q000235:genetics / Q000502:physiology; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000235:genetics / Q000502:physiology; D014667:Vasopressins / Q000097:blood / Q000502:physiology; D014868:Water Deprivation / Q000502:physiology*
C496024:Aqp2 protein, mouse; D051399:Aquaporin 2; D018076:DNA, Complementary; D053503:Epithelial Sodium Channels; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors; C499496:Pax8 protein, mouse; D012333:RNA, Messenger; D017483:Receptors, Vasopressin; D015222:Sodium Channels; D014667:Vasopressins; D051400:Aquaporin 3; D015096:3-Hydroxysteroid Dehydrogenases; C511890:3beta-hydroxysteroid dehydrogenase 4, mouse; D005634:Fructose-Bisphosphate Aldolase; D000254:Sodium-Potassium-Exchanging ATPase