A mass spectrometry compatible silver staining method for protein incorporating a new silver sensitizer in sodium dodecyl sulfate-polyacrylamide electrophoresis gels.
A quick, sensitive, and MALDI-TOF MS compatible silver staining method, namely Eriochrome black T (EBT)-silver method, is described. The method can detect 0.05-0.2 ng protein within 60 min in SDS-PAGE gels. EBT dye was used as a silver ion sensitizer having reducing power for silver ions.
Jin
Li-Tai
LT
College of Pharmacy, Chonnam National University, Kwangju, South Korea.
Hwang
Sun-Young
SY
Yoo
Gyurng-Soo
GS
Choi
Jung-Kap
JK
D001391:Azo Compounds / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation* / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods*; D016622:Silver Staining / Q000379:methods*
D001391:Azo Compounds; C014527:Eriochrome Black T