Normal patterns of expression of glycosylphosphatidylinositol-anchored proteins on different subsets of peripheral blood cells: a frame of reference for the diagnosis of paroxysmal nocturnal hemoglobinuria.
BACKGROUND
Evaluation of the expression of glycosylphosphatidylinositol-anchored membrane proteins (GPI-AP) is currently used for the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH). In this study, we analyzed the amount of expression of a wide variety of GPI-AP in different subsets of hematopoietic cells present in normal peripheral blood (PB), to establish their normal patterns of expression and provide a frame of reference for the definition of the best combination of GPI-AP and PB cell subsets to be applied in the diagnosis and monitoring of PNH.


RESULTS
Our results show variable patterns of expression of different GPI-AP in distinct subsets of normal PB cells. Combined use of CD55 and CD59 represented the most useful dual-marker combination; however, its utility remained suboptimal for several subsets of leukocytes and for platelets.


CONCLUSIONS
For some cell subsets such as the neutrophils additional useful markers could be selected from a relatively broad panel (CD16/CD24/CD55/CD59/CD66b/CD157), whereas for other cell subsets the number of useful antigens was either restricted (monocytes: CD14/CD55/CD157; B cells: CD24/CD48/CD52/CD55; CD4+ T cells: CD48/CD52/CD55; eosinophils: CD55/CD59; CD8+ T cells: CD48/CD55) or limited to a single marker (CD48 on CD56low NK cells, CD55 on BDCA3- dendritic cells and CD56high NK cells, and CD59 for red cells), from all antigens analyzed.
Servicio General de Citometría, Centro de Investigación del Cáncer, Departamento de Medicina, Universidad de Salamanca, Salamanca, Spain.
Pilar María
PM
Hernández-Campo
Julia
J
Almeida
María Luz
ML
Sánchez
Mariester
M
Malvezzi
Alberto
A
Orfao
D015703:Antigens, CD / Q000097:blood; D000951:Antigens, Neoplasm / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000737:chemistry / Q000276:immunology; D000071178:CD48 Antigen; D000074301:CD52 Antigen; D018958:CD55 Antigens / Q000097:blood; D018968:CD58 Antigens / Q000097:blood; D018957:CD59 Antigens / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000097:blood; D017261:Glycosylphosphatidylinositols / Q000097:blood*; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000145:classification*; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood* / Q000175:diagnosis*; D006801:Humans; D016130:Immunophenotyping; D007962:Leukocytes / Q000737:chemistry / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012015:Reference Standards
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D015415:Biomarkers; D000071178:CD48 Antigen; C000604065:CD48 protein, human; D000074301:CD52 Antigen; C000613862:CD52 protein, human; D018958:CD55 Antigens; D018968:CD58 Antigens; D018957:CD59 Antigens; D006023:Glycoproteins; D017261:Glycosylphosphatidylinositols