[Effect of Huajiao volatile oil on Cu2+ -induced oxidative modification of low density lipoprotein in vitro].
OBJECTIVE
To evaluate the effect of Huajiao volatile oil on copper mediated low density lipoprotein (LDL) oxidative modification.


METHODS
LDL was isolated by ultracenfugation from normal human plasma. The extent of LDL peroxidation was measured in terms of thiobarbituric acid reactive substances (TBARS) expressed as malondialdehyde (MDA) equivalents. The electrophoresis mobility of LDL was determined as relative electrophoresis mobility (REM) on an agarose gel electrophoresis.


RESULTS
The TBARS generation and the REM in Cu2+ -induced oxidation of LDL with Huajiao volatile oil at different concentrations were statistically different (P < 0.01). The Cu2+ -induced oxidation of LDL without addition of Huajiao volatile oil exhibited a lag phase of 1 h, a rapid increasing phase at 2 h-4 h, and a summit phase starting at 24 h. Then, with the addition of Huajiao volatile oil (40 mg/mL), the lag phase lasted 4 h, the rapid increasing phase was seen at 6 h-8 h, but the summit of TBARS was not on the decline.


CONCLUSIONS
Huajiao volatile oil could protect LDL against Cu2+ -induced oxidative modification in vitro.
Department of Thoracic and Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Jian-yang
JY
Ma
Ying-kang
YK
Shi
Ding-zhi
DZ
Fang
D000975:Antioxidants / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008075:Lipoproteins, HDL / Q000097:blood / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D009822:Oils, Volatile / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D032106:Zanthoxylum / Q000737:chemistry*
D000975:Antioxidants; D008075:Lipoproteins, HDL; D009822:Oils, Volatile; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D003300:Copper