Effect of nuclear factor kappa B on intercellular adhesion molecule-1 expression and neutrophil infiltration in lung injury induced by intestinal ischemia/reperfusion in rats.
OBJECTIVE
To investigate the role of nuclear factor kappa B (NF-kappaB) in the pathogenesis of lung injury induced by intestinal ischemia/reperfusion (I/R), and its effect on intercellular adhesion molecule-1 (ICAM-1) expression and neutrophil infiltration.


METHODS
Twenty-four Wistar rats were divided randomly into control, I/R and pyrrolidine dithiocarbamate (PDTC) treatment groups, n=8 in each. I/R group and PDTC treatment group received superior mysenteric artery (SMA) occluding for 1 h and reperfusion for 2 h. PDTC group was administrated with intraperitoneal injection of 2% 100 mg/kg PDTC 1 h before surgery. Lung histology and bronchia alveolus lung fluid (BALF) protein were assayed. Serum IL-6, lung malondialdehyde (MDA) and myeloperoxidase (MPO) as well as the expression level of NF-kappaB and ICAM-1 were measured.


RESULTS
Lung injury induced by intestinal I/R, was characterized by edema, hemorrhage and neutrophil infiltration as well as by the significant rising of BALF protein. Compared to control group, the levels of serum IL-6 and lung MDA and MPO increased significantly in I/R group (P=0.001). Strong positive expression of NF-kappaB p65 and ICAM-1 was observed. After the administration of PDTC, the level of serum IL-6, lung MDA and MPO as well as NF-kappaB and ICAM-1 decreased significantly (P<0.05) when compared to I/R group.


CONCLUSIONS
The activation of NF-kappaB plays an important role in the pathogenesis of lung injury induced by intestinal I/R through upregulating the neutrophil infiltration and lung ICAM-1 expression. PDTC as an inhibitor of NF-kappaB can prevent lung injury induced by intestinal I/R through inhibiting the activity of NF-kappaB.
Department of General Surgery, Second Affiliated Hospital, Dalian Medical University, Dalian 116027, Liaoning Province, China. txfdl@tom.com
Xiao-Feng
XF
Tian
Ji-Hong
JH
Yao
Ying-Hua
YH
Li
Xue-Song
XS
Zhang
Bing-An
BA
Feng
Chun-Ming
CM
Yang
Shu-Sen
SS
Zheng
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D015850:Interleukin-6 / Q000097:blood; D007422:Intestines / Q000098:blood supply*; D008168:Lung / Q000276:immunology / Q000473:pathology*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D020556:Neutrophil Infiltration; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
D015850:Interleukin-6; D016328:NF-kappa B; D018799:Intercellular Adhesion Molecule-1