[Genetic analysis and RAPD marker of the genes for brachytic stem trait in soybean].
Three crosses between NG94-156 (brachytic stem) and three varieties (normal stem) were made, and F2 segregative population and two recombined inbred line populations(F(7:8)) were obtained. Genetic analysis indicated that the brachytic stem of NG94-156 was controlled by two duplicate recessive genes. In searching for RAPD marker linked to the genes controlling brachytic stem, 260 RAPD primers were applied to screen four parents of three combinations and RIL. Polymorphic bands revealed by the primer S-506 exhibited the best repeatability among all primers. Linkage analysis indicated the genetic distance between S-506(1600) and brachytic stem gene was 6.94 cM.
National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. fhuang@njau.edu.cn
Fang
F
Huang
Tuan-Jie
TJ
Zhao
Qing-Chang
QC
Meng
Yuan-Ming
YM
Zhang
Jun-Yi
JY
Gai
De-Yue
DY
Yu
D008040:Genetic Linkage / Q000235:genetics; D018547:Plant Stems / Q000235:genetics* / Q000254:growth & development*; D011110:Polymorphism, Genetic / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development*