Coupling of enantioselective biooxidation of DL-1,2-propanediol and bioreduction of pinacolone via regeneration cycle of coenzyme.
Enantioselective biotransformation of DL-1,2-propanediol to D-2-hydroxypropanic acid was first reported by the authors. In the biooxidation process, there were some by-product formed and thus influenced the e.e. value and output of the acid. Restricting oxygen in the reaction system and offering additional proton receptor to the system displayed approving effect. The latter method constructed regeneration cycle system of coenzyme. In the article, the bioreduction of pinacolone was coupled to the enantioselective oxidation. Yield of the acid was increased by 36% and e.e. value of the product approached 99%.
State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 200237, Shanghai, China.
Keliang
K
Gao
Qingxun
Q
Song
Dongzhi
D
Wei
D001711:Biotransformation; D002074:Butanones / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003067:Coenzymes / Q000378:metabolism; D020576:Gluconobacter / Q000166:cytology / Q000378:metabolism*; D008954:Models, Biological; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011408:Propanediol Dehydratase / Q000378:metabolism; D019946:Propylene Glycol / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D013696:Temperature
D002074:Butanones; D003067:Coenzymes; C084935:pinacolone; D019946:Propylene Glycol; D011408:Propanediol Dehydratase