[Molecular cloning of a novel low-molecular-weight glutenin subunit gene from wheat variety "Chuanmai 42"].
Using PCR-amplification method, the coding sequences of a novel low-molecular-weight glutenin (LMW-GS) gene, LMWCM42-1, was isolated from the genomic DNA of wheat (Triticum aestivum L.) variety 'Chuanmai 42'. The full coding region of this gene consisted of 846 nucleotides, and encoded a protein with 281 amino acids. LMWCM42-1 is a typical low-molecular-weight glutenin subunit (LMW-GS) gene. In spite of high similarity with the known LMW-GSs, LMWCM42-1 was quite different from the known LMW-GS in the N-terminal, central repetitive domain and C-terminal domain. It was found that LMWCM42-1 was located within the Glu-D3 locus by phylogenetic analysis.
Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, China.
Lei
L
Shen
Hai
H
Long
Ze-Hong
ZH
Yan
Yu-Ming
YM
Wei
You-Liang
YL
Zheng
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D005983:Glutens / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics*
D005983:Glutens; C037302:glutenin