Tumor cell migration in three dimensions.
In almost all physiological and pathological situations, cells migrate through three-dimensional environments, yet most studies of cell motility have used two-dimensional substrates. It is clear that two-dimensional substrates do not mimic the in vivo environment accurately, and recent work using three-dimensional environments has revealed many different mechanisms of cell migration (Abbott, 2003; Sahai and Marshall, 2003; Wolf et al., 2003). This chapter will describe methods for generating three-dimensional matrices suitable for studying cell motility, methods for imaging the morphology of motile cells in situ, and methods for quantifying cell migration through three-dimensional environments.
Tumour Cell Biology Laboratory, Cancer Research United Kingdom London Research Institute.
Steven
S
Hooper
John F
JF
Marshall
Erik
E
Sahai
D000577:Amides / Q000494:pharmacology; D000818:Animals; D002294:Carcinoma, Squamous Cell; D018929:Cell Culture Techniques; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D003094:Collagen / Q000378:metabolism / Q000648:ultrastructure; D024042:Collagen Type I / Q000648:ultrastructure; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000502:physiology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007797:Laminin / Q000378:metabolism / Q000648:ultrastructure; D008545:Melanoma; D018613:Microscopy, Confocal / Q000379:methods; D008855:Microscopy, Electron, Scanning; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors; D011509:Proteoglycans / Q000378:metabolism / Q000648:ultrastructure; D011725:Pyridines / Q000494:pharmacology; D054460:rho-Associated Kinases
D000577:Amides; D024042:Collagen Type I; D004338:Drug Combinations; D047908:Intracellular Signaling Peptides and Proteins; D007797:Laminin; D011509:Proteoglycans; D011725:Pyridines; C059485:matrigel; C108830:Y 27632; D003094:Collagen; D017346:Protein Serine-Threonine Kinases; D054460:rho-Associated Kinases