Preferential radioprotection to DNA of normal tissues by ferulic acid under ex vivo and in vivo conditions in tumor bearing mice.
Our previous study showed that ferulic acid (FA) offered good radioprotection under in vitro and in vivo conditions to DNA and enhanced the DNA repair process in the peripheral blood leucocytes of mice in vivo. This study concerns radioprotection of normal versus tumor cells. Administration of FA (50 mg/kg body weight) to mice bearing fibrosarcoma tumor, 1 h prior to/ or immediately after radiation exposure (4 Gy) showed preferential radioprotection to normal cells i.e. peripheral blood leucocytes and bone marrow cells in comparison to tumor cells. This preferential protection under in vivo conditions could be attributed to poor vasculature in the tumor or peculiar characteristics of the tumor cells either to restrict its entry inside the cells or metabolize or inactivate the drug. To resolve these ex vivo study was carried out using bone marrow and tumor cells. It was found that under ex vivo condition also only bone marrow cells were protected by FA. Thus the studies revealed that FA showed preferential protection to normal cells under both in vivo and ex vivo conditions.
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, 400 085, Maharashtra, India.
Dharmendra Kumar
DK
Maurya
Cherupally Krishnan Krishnan
CK
Nair
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects; D020552:Comet Assay; D003373:Coumaric Acids / Q000494:pharmacology*; D004247:DNA / Q000097:blood / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000097:blood / Q000187:drug effects / Q000528:radiation effects*; D005354:Fibrosarcoma / Q000188:drug therapy / Q000532:radiotherapy*; D005720:Gamma Rays / Q000627:therapeutic use; D007962:Leukocytes / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D003373:Coumaric Acids; D004273:DNA, Neoplasm; D011837:Radiation-Protective Agents; D004247:DNA; C004999:ferulic acid