Identification and characterization of Trichoplusia ni furin truncated mutant.
We report a new prohormone convertase gene of insect origin, Trichoplusia ni furin, which was cloned from BTI-Tn-5B-4 (Tn5) insect cells. We constructed a truncated mutant that lacked Cys-rich repeated segments. Using baculovirus expression system and standard enzymatic assay, we obtained recombinant Tn furin and evaluated aspects of its function.
Wang
Lie
L
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200031, P.R.China.
Xu
Dan
D
Qian
Ming
M
Yang
Guanzhen
G
Wu
Xiangfu
X
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000737:chemistry / Q000494:pharmacology; D002413:Cations, Divalent / Q000737:chemistry; D002460:Cell Line; D003001:Cloning, Molecular; D003470:Culture Media; D045683:Furin / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009036:Moths / Q000235:genetics* / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D011489:Protein Denaturation; D013696:Temperature
D002413:Cations, Divalent; D003470:Culture Media; D045683:Furin; D002118:Calcium