Differential effects of drug-induced ascorbic acid release in the striatum and nucleus accumbens of freely moving rats.
Previous studies have shown that striatum and nucleus accumbens (NAc) are two different structures in mediating addictive drug-induced ascorbic acid (AA) release. In order to further characterize the different effects of drugs-induced AA release in the striatum and NAc, in the present study, we investigated the effect of ethanol, morphine, methamphetamine, nicotine-induced AA release in these two nuclei using microdialysis coupled to high performance liquid chromatography with electrochemical detection (HPLC-ECD). All drugs were continuously perfused directly into the striatum or NAc. This study showed that local intrastriatal or intra-accumbensal perfusion of ethanol (500 microM) could increase AA release to 280, 260% in the striatum and NAc, respectively. Intra-striatal infusion of morphine (1 mM), methamphetamine (250 microM) or nicotine (500 microM), reduce striatal AA release to 48, 50, 45%, respectively. While given intra-accumbensally, morphine (1 mM), methamphetamine (250 microM) or nicotine (500 microM) increase AA release to 165, 160, 160%, respectively. These results suggested that different presynaptic or postsynaptic mechanisms might be involved in addictive drug-induced AA release in the striatum and NAc.
Department of Pharmacology, Shenyang Pharmaceutical University, Wenhua Road 103, 110016 Shenyang, PR China.
Pei Fei
PF
Gu
Chun Fu
CF
Wu
Jing Yu
JY
Yang
Yu
Y
Shang
Yue
Y
Hou
Xiu Li
XL
Bi
Fang
F
Dai
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000378:metabolism*; D000431:Ethanol / Q000009:adverse effects; D008297:Male; D008694:Methamphetamine / Q000009:adverse effects; D017551:Microdialysis; D009020:Morphine / Q000009:adverse effects; D009538:Nicotine / Q000009:adverse effects; D009714:Nucleus Accumbens / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D019966:Substance-Related Disorders / Q000378:metabolism*
D000431:Ethanol; D008694:Methamphetamine; D009538:Nicotine; D009020:Morphine; D001205:Ascorbic Acid