Breast cancer proteomics using two-dimensional electrophoresis: studying the breast cancer proteome.
Proteomics has emerged as a powerful approach for studying disease-associated changes in protein levels. The most commonly used method in proteomics studies remains two-dimensional gel electrophoresis, but the methodology, standardization, and interpretation of the results obtained require considerable expertise. In this chapter, we describe the approaches we have taken to studying the breast cancer proteome, using cells grown in vitro and cancer specimens as the starting materials.
Dwek
Miriam V
MV
Department of Applied and Molecular Biosciences, School of Biosciences, University of Westminster, London, UK.
Rawlings
Sarah L
SL
D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D009363:Neoplasm Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
D009363:Neoplasm Proteins; D020543:Proteome