[Study on transformation of P-dissolving Penicillium oxalicum P8 with double-marker vector expressing green fluorescent protein and hygromycin B resistance].
P-dissolving Penicillium oxalicum P8 was isolated previously in this lab which has a considerable ability to dissolve many kinds of inorganic phosphorus and improve crop growth. In order to study rhizosphere colonization of plants by Penicillium oxalicum P8, protoplasts were transformed with a double-marker expression vector of green fluorescent protein and hygromycin B resistance. Some transformants were selected which expressed both the GFP and hygromycin B phosphotransferase and did not show significant morphological or physiological differences as compared to wild-type strain. Southern blot analysis confirmed the heterogeneous genomic integration of the vector DNA into the transformants.
Zhang
Lei
L
Department of Life Science, Nanjing Agriculture University, Nanjing 210095, China. zhanglei_75@163.com
Fan
Bing-Quan
BQ
Huang
Wei-Yi
WY
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006921:Hygromycin B / Q000494:pharmacology*; D010407:Penicillium / Q000235:genetics* / Q000378:metabolism; D010758:Phosphorus / Q000737:chemistry*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D012995:Solubility; D014169:Transformation, Bacterial
D000900:Anti-Bacterial Agents; D049452:Green Fluorescent Proteins; D010758:Phosphorus; D006921:Hygromycin B; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase