[Rapid determination of transient expression characteristics of drought- and dehydration-inducible promoters from plants].
The selection of suitable inducible-promoters is one of the most important chains in transgenic researches for plant tolerances to drought, dehydration and other abiotic stresses. Based on laboratory researches in the past years, it was set up a set of methods that could be used to rapidly determine the transient expression characteristics of drought- and dehydration-inducible promoters by using whole barley seedling and plant leaves in vitro as bombardment objects. Promoters Dhn4s, Dhn8s, HVA1s, Rab16Bj and wsi18j isolated from barley and rice were able to express GFP in the leaves of barley, wheat, rice, sorghum and fern after drying treatment, but not in laves of mungbean and tomato. The qualitative expression of HVA1s and wsi18j in different organs and tissues of barley was identified. A method for quantitative analysis of promoter transient expression was established by means of GFP foci/GUS foci counting or GUS activity/XYN activity test. Finally, the value and prospect of the methodology were discussed in terms of its application to the analysis of plant promoters inducible by environmental factors.
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. fhxiao@mail.ynau.edu.cn
Feng-Hui
FH
Xiao
Cheng-Li
CL
Duan
Hu-Qu
HQ
Zhai
Gui-Ping
GP
Xue
Hong-Sheng
HS
Zhang
D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D001467:Hordeum / Q000235:genetics; D012275:Oryza / Q000235:genetics; D010944:Plants / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D036226:Seedlings / Q000235:genetics