Monoclonal MOG-reactive autoantibody from progressive EAE has the characteristics of a natural antibody.
A.SW mice sensitized with myelin oligodendrocyte glycoprotein (MOG)92-106 is an animal model for progressive multiple sclerosis (MS). We isolated MOG-reactive monoclonal antibodies that were immunoglobulin (Ig)M and polyreactive, similar to natural autoantibodies. Upon analysis of the variable (V) light chains and the diversity (D) and joining (J) regions of V heavy chains, we found they were identical to germ line Vkappa19/28, Jkappa5, DFL16.1e and JH4, respectively. The sequence of the VH region had 99.7% and 100% identity at the nucleotide and amino acid levels, respectively, compared with the germ line encoded antibody, P3, of the Q52 family. Although A strain mice have been reported to have an insertion in BAFF-R, the receptor for BAFF (B cell activation factor from the tumor necrosis factor family), which could explain our results, A.SW mice have no mutations in BAFF-R.
Department of Neurology, University of Utah School of Medicine, 30 North 1900 East, 3R330 SOM, Salt Lake City, UT 84132-2305, USA.
Jane E
JE
Libbey
Lisa K
LK
Peterson
Ikuo
I
Tsunoda
Robert S
RS
Fujinami
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D001323:Autoantibodies / Q000235:genetics*; D053265:B-Cell Activation Factor Receptor; D001483:Base Sequence; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005803:Genes, Immunoglobulin; D006825:Hybridomas; D007075:Immunoglobulin M / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D020297:Mice, Congenic; D008969:Molecular Sequence Data; D009154:Mutation; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein; D016133:Polymerase Chain Reaction; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics; D017385:Sequence Homology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D053265:B-Cell Activation Factor Receptor; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D008565:Membrane Proteins; C571327:Mog protein, mouse; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D018124:Receptors, Tumor Necrosis Factor; C486662:Tnfrsf13c protein, mouse