Analysis of isomeric tropane alkaloids from Schizanthus grahamii by very fast gas chromatography.
This study presents a very fast GC analysis applied for the baseline separation of isomeric tropane alkaloids extracted from the stem-bark of Schizanthus grahamii (Solanaceae). The work provided a challenging application where isothermal analysis in conjunction with very short narrow bore columns (3 m x 100 microm ID and 1.5 m x 50 microm ID) was particularly suited for the speeding up. Experimental parameters were used in the optimisation steps, including selection of stationary phase, temperature, internal column diameter and optimal practicable gas velocity. Some considerations about sample injection in fast isothermal analysis are also briefly presented. Finally, the investigated approach allowed a very fast baseline separation of four positional and configurational isomers in less than 9 s.
Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences EPGL, University of Geneva, Geneva, Switzerland.
Stefan
S
Bieri
Orlando
O
Muñoz
Jean-Luc
JL
Veuthey
Philippe
P
Christen
D002849:Chromatography, Gas / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D007536:Isomerism; D008968:Molecular Conformation; D012828:Silicones / Q000737:chemistry; D019657:Solanaceae / Q000737:chemistry*; D014326:Tropanes / Q000032:analysis* / Q000737:chemistry
D004129:Dimethylpolysiloxanes; D012828:Silicones; D014326:Tropanes; C078211:polymethylsiloxane