Synthesis of a bis-azido analogue of acromelic acid for radioisotope-free photoaffinity labeling and biochemical studies.
A novel acromelic acid analogue containing a phenyl group possessing two different types of azido functional groups, of which one is the aromatic N3 acting as a photoaffinity group to bind to a target protein by photoirradiation and the other is alkyl N3 group which survives photolysis acting as a detecting group through the Staudinger-Bertozzi reaction to identify the ligated product, was designed and synthesized as a radioisotope-free biochemical probe potentially for studies on kainoid receptors.
Regeneration and Advanced Medical Science, Graduate School of Medicine, Gifu University, Gifu 501-1193, Japan.
Pi
P
Sun
Guang Xing
GX
Wang
Kyoji
K
Furuta
Masaaki
M
Suzuki
D001386:Azides / Q000737:chemistry*; D015195:Drug Design; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D008968:Molecular Conformation; D019920:Photoaffinity Labels / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D011506:Proteins / Q000737:chemistry / Q000187:drug effects; D018092:Kainic Acid Receptors / Q000737:chemistry / Q000187:drug effects; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
D001386:Azides; D007608:Kainic Acid; D019920:Photoaffinity Labels; D011506:Proteins; D018092:Kainic Acid Receptors; C051743:acromelic acid A