Preparation and evaluation of unimolecular pentavalent and hexavalent antigenic constructs targeting prostate and breast cancer: a synthetic route to anticancer vaccine candidates.
Several novel, fully synthetic, carbohydrate-based antitumor vaccines have been assembled. Each construct consists of multiple cancer-related antigens displayed on a single polypeptide backbone. Recent advances in synthetic methodology have allowed for the incorporation of a complex oligosaccharide terminating in a sialic acid residue (i.e., GM2) as one of the carbohydrate antigens. Details of the vaccine synthesis as well as the results of preliminary immunological investigations are described herein.
Ragupathi
Govind
G
Laboratory of Tumor Vaccinology, Clinical Immunology Service, Department of Medicine, the Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, New York, New York 10021, USA.
Koide
Fusataka
F
Livingston
Philip O
PO
Cho
Young Shin
YS
Endo
Atsushi
A
Wan
Qian
Q
Spassova
Maria K
MK
Keding
Stacy J
SJ
Allen
Jennifer
J
Ouerfelli
Ouathek
O
Wilson
Rebecca M
RM
Danishefsky
Samuel J
SJ
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology* / Q000628:therapy; D019496:Cancer Vaccines / Q000138:chemical synthesis* / Q000276:immunology; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D005260:Female; D005678:G(M2) Ganglioside / Q000737:chemistry / Q000276:immunology; D006027:Glycosides / Q000737:chemistry; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011471:Prostatic Neoplasms / Q000276:immunology* / Q000628:therapy; D012794:Sialic Acids / Q000737:chemistry
D000596:Amino Acids; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D006027:Glycosides; D009844:Oligosaccharides; D010455:Peptides; D012794:Sialic Acids; D005678:G(M2) Ganglioside; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin