Energetics of osmoregulation: I. Oxygen consumption by Fundulus heteroclitus.
We have developed a flow-through method for measuring oxygen consumption in fish which allows continuous monitoring over periods of days with good accuracy. Our goal was to determine the changes in basal metabolic rate in estuarine fish as a function of salinity. We show that in Fundulus heteroclitus, the oxygen consumption drops by 50% during the first 12 hr in the respirometer, as the fish cease exploratory movements. We have determined the influence of temperature and body size on resting respiratory rate, but failed to find any circadian or tidal rhythm in aerobic respiration. With these variables controlled, we determined that changing from 10 to 30 ppt water had no demonstrable effect on oxygen uptake. Since there must be a large change in osmotic flux due to this change in salinity, it appears that the fish might be diverting energy from other uses rather than increasing aerobic energy production to meet the increased osmoregulatory work load.
Kidder
George W
GW
Mt. Desert Island Biological Laboratory, Salisbury Cove, Maine 04672, USA. gkidder@mdibl.org
Petersen
Christopher W
CW
Preston
Robert L
RL
D000818:Animals; D049628:Body Size; D002940:Circadian Rhythm; D004734:Energy Metabolism; D023781:Fundulidae / Q000502:physiology*; D008991:Monitoring, Physiologic; D009995:Osmosis; D010101:Oxygen Consumption; D012119:Respiration; D013696:Temperature; D013997:Time Factors; D014882:Water-Electrolyte Balance