A serial copolymerization procedure for manufacturing immunoadsorption walls as a potential unit in conjunction with hemodialysis filters.
A functional immunoadsorption wall for removal of beta-2-microglobulin has been made by partially incomplete two-stage copolymerization of acrylamide with immunoadsorbent. However, a substantial amount of immunoadsorbent needs to be flushed away after the copolymerization process. Thus, to enhance the utilization efficiency of immunoadsorbent, the flushed-away immunoadsorbent was further recovered, and the copolymerization was conducted in series to produce three consecutive immunoadsorption walls in this study. Preliminary removal tests show that similar removal patterns were obtained for these immunoadsorption walls. Although it is not timely to conclude that a clinically applicable immunoadsorption wall has taken shape, the development of a partially incomplete two-stage polymerization method and its associated techniques indeed provide a good basis for large-scale manufacturing immunoadsorption walls.
Yang
Tsung-Hua
TH
Department of Chemical Engineering, Cheng Shiu University, Kaohsiung, Taiwan. thyang@csu.edu.tw
Fang
Chi-Lin
CL
D020106:Acrylamide / Q000494:pharmacology; D000818:Animals; D004867:Equipment Design; D006801:Humans; D007163:Immunosorbent Techniques / Q000295:instrumentation*; D007164:Immunosorbents / Q000494:pharmacology; D006435:Renal Dialysis; D001613:beta 2-Microglobulin / Q000652:urine
D007164:Immunosorbents; D001613:beta 2-Microglobulin; D020106:Acrylamide