Dissimilar kinetic behavior of electrically manipulated single- and double-stranded DNA tethered to a gold surface.
We report on the electrical manipulation of single- and double-stranded oligodeoxynucleotides that are end tethered to gold surfaces in electrolyte solution. The response to alternating repulsive and attractive electric surface fields is studied by time-resolved fluorescence measurements, revealing markedly distinct dynamics for the flexible single-stranded and stiff double-stranded DNA, respectively. Hydrodynamic simulations rationalize this finding and disclose two different kinetic mechanisms: stiff polymers undergo rotation around the anchoring pivot point; flexible polymers, on the other hand, are pulled onto the attracting surface segment by segment.
Walter Schottky Institute, Technical University Munich, 85748 Garching, Germany. rant@wsi.tum.de
Ulrich
U
Rant
Kenji
K
Arinaga
Marc
M
Tornow
Yong Woon
YW
Kim
Roland R
RR
Netz
Shozo
S
Fujita
Naoki
N
Yokoyama
Gerhard
G
Abstreiter
D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D006046:Gold / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D011829:Radiation Dosage
D006046:Gold; D004247:DNA