Assessment of cystic fibrosis transmembrane conductance regulator (CFTR) activity in CFTR-null mice after bone marrow transplantation.
Several studies have demonstrated that bone marrow (BM)-derived cells give rise to rare epithelial cells in the gastrointestinal (GI) and respiratory tracts after BM transplantation into myeloablated recipients. We investigate whether, after transplantation of cystic fibrosis transmembrane conductance regulator (CFTR)-positive BM-derived cells, BM-derived GI and airway epithelial cells can provide CFTR activity in the GI tract and nasal epithelium of recipient cystic fibrosis mice. CFTR-/- mice were transplanted with wild-type BM after receiving different doses of irradiation, and CFTR activity was assessed in vivo in individual mice over time by using rectal and nasal potential difference analyses and in vitro by Ussing chamber analysis. The data suggest that rare BM-derived epithelial cells in the GI and nasal epithelium detected in CFTR-/- transplanted mice provide a modest level of CFTR-dependent chloride secretion. Detection of CFTR mRNA and protein in tissues of transplanted CFTR-/- mice supports these data.
Department of Laboratory Medicine, Yale University School of Medicine, 330 Cedar Street, New Haven, CT 06520, USA.
Emanuela M
EM
Bruscia
Joanna E
JE
Price
Ee-Chun
EC
Cheng
Scott
S
Weiner
Christina
C
Caputo
Elisa C
EC
Ferreira
Marie E
ME
Egan
Diane S
DS
Krause
D000818:Animals; D016026:Bone Marrow Transplantation; D003550:Cystic Fibrosis / Q000628:therapy*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000032:analysis* / Q000235:genetics; D005753:Gastric Mucosa / Q000737:chemistry; D015316:Genetic Therapy; D007413:Intestinal Mucosa / Q000737:chemistry; D051379:Mice; D018181:Mice, Inbred CFTR; D009297:Nasal Mucosa / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D019005:Cystic Fibrosis Transmembrane Conductance Regulator