X-gal inhibits the swarming of Vibrio species.
The expressional levels of genes in swarmer cells can be determined by a simple method using X-gal-containing semisolid agars and lacZ-fusion transcription reporter strains of the genes concerned. However, X-gal alone inhibited the swarming of Vibrio, regardless of their ability to digest X-gal. Moreover, X-gal inhibited the growth of V. vulnificus containing functional lacZ. These effects of X-gal itself should be carefully considered when trying to determine the expression levels of genes in swarming cells using X-gal-containing semisolid agar.
Kim
Moon-Young
MY
Research Center for Resistant Cells, Chosun University Medical School, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
Park
Ra-Young
RY
Bai
Young-Hoon
YH
Chung
Yoon-Young
YY
Kim
Choon-Mee
CM
Kim
Soo-Young
SY
Rhee
Joon-Haeng
JH
Shin
Sung-Heui
SH
D002863:Chromogenic Compounds / Q000494:pharmacology*; D005697:Galactosides / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D014733:Vibrio / Q000187:drug effects* / Q000502:physiology*
D002863:Chromogenic Compounds; D005697:Galactosides; D007211:Indoles; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside