Spectrally resolved bioluminescence optical tomography.
Spectrally resolved bioluminescence optical tomography is an approach to recover images of luciferase activity within a volume using multiwavelength emission data from internal bioluminescence sources. The underlying problem of uniqueness associated with nonspectrally resolved intensity-based bioluminescence tomography is highlighted. Reconstructed images of bioluminescence are presented by using as input both simulated and real multiwavelength data from a tissue-simulating phantom. The location of the internal bioluminescence is obtained with 1 mm accuracy. Further, the amplitude of the reconstructed source is proportional to the actual bioluminescence intensity.
Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, USA.
Hamid
H
Dehghani
Scott C
SC
Davis
Shudong
S
Jiang
Brian W
BW
Pogue
Keith D
KD
Paulsen
Michael S
MS
Patterson
D021621:Imaging, Three-Dimensional / Q000379:methods*; D008156:Luciferases / Q000032:analysis*; D008163:Luminescent Measurements / Q000379:methods*; D008164:Luminescent Proteins / Q000032:analysis*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D041622:Tomography, Optical / Q000379:methods*
D008164:Luminescent Proteins; D008156:Luciferases