Evaluation of two GnRH-I based vaccine formulations on the testes function of entire Suffolk cross ram lambs.
A modified GnRH peptide (CHWSYGLRPG-NH(2)) was conjugated to tetanus toxoid (TT) or diphtheria toxoid (DT) and formulated with Quil A saponin or a sustained release injectible PLGA (poly(lactide-co-glycolide)/triacetin). For the Quil A formulations, two administrations of TT conjugate at 3-weekly intervals were followed by two booster injections with the DT conjugate in entire ram lambs. With the PLGA formulations, only two injections were administered; the first containing TT and the second DT at 6-weekly intervals. Evaluation was carried out by comparing the specific antibody levels produced in relationship to hormone profiles and testicular changes. The Quil A formulation was considered the most effective, as it caused significant reduction in testosterone and follicle stimulating hormone levels, resulting in marked suppression of spermatogenesis.
Earl
Elizabeth R
ER
Novartis Animal Vaccines Ltd., Springwood Industrial Estate, Braintree, Essex CM7 2YW, UK.
Waterston
Mary M
MM
Aughey
Elizabeth
E
Harvey
Michael J A
MJ
Matschke
Christian
C
Colston
Angela
A
Ferro
Valerie A
VA
D000276:Adjuvants, Immunologic; D000818:Animals; D000906:Antibodies / Q000097:blood; D003269:Contraception, Immunologic / Q000379:methods*; D004168:Diphtheria Toxoid / Q000008:administration & dosage / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005640:Follicle Stimulating Hormone / Q000097:blood; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry / Q000276:immunology*; D006651:Histocytochemistry; D007115:Immunization Schedule; D019344:Lactic Acid; D008297:Male; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D062605:Quillaja Saponins; D012503:Saponins / Q000276:immunology; D012756:Sheep / Q000502:physiology*; D013091:Spermatogenesis; D013735:Testicular Hormones / Q000097:blood; D013737:Testis / Q000166:cytology; D013739:Testosterone / Q000097:blood; D013745:Tetanus Toxoid / Q000008:administration & dosage / Q000276:immunology; D018074:Vaccines, Conjugate / Q000737:chemistry / Q000276:immunology; D022642:Vaccines, Contraceptive / Q000008:administration & dosage*
D000276:Adjuvants, Immunologic; D000906:Antibodies; D004168:Diphtheria Toxoid; D011108:Polymers; D062605:Quillaja Saponins; D012503:Saponins; D013735:Testicular Hormones; D013745:Tetanus Toxoid; D018074:Vaccines, Conjugate; D022642:Vaccines, Contraceptive; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D007987:Gonadotropin-Releasing Hormone; D019344:Lactic Acid; D013739:Testosterone; C046386:Quil A; D005640:Follicle Stimulating Hormone