Lack of in vitro genotoxicity of povidone-iodine in solution, in ointment or in a liposomal formulation (Repithel).
The in vitro genotoxicity of povidone-iodine in Repithel, Betaisodona ointment and Betaisodona solution was investigated using CHO-K1 cells. The comet assay and chromosome aberration test were used to characterise the genotoxic potency of povidone-iodine within 4 h of contact with test cells. Methyl methanesulphonate without S9 activation and benzo[a]pyrene with S9 activation were used as positive controls. No higher concentrations than 6% Repithel, 5% Betaisodona ointment and 2% Betaisodona solution in culture medium with 10% fetal bovine serum were tested within 4 h of contact with CHO-K1 cells for the comet assay, because higher concentrations will provide >60% cytotoxicity. All tested concentrations of povidone-iodine formulations (4-6% Repithel, 3-5% Betaisodona ointment and 1-2% Betaisodona solution) are not genotoxic in the comet assay. 10% Repithel, 5% Betaisodona ointment and 2.5% Betaisodona solution in culture medium with 10% fetal bovine serum were final concentrations in the chromosome aberration test, which provide <60% cytotoxicity within 4 h of contact with CHO-K1 cells. All tested concentrations of povidone- iodine formulations (5-10% Repithel, 2.5-5% Betaisodona ointment and 1.25-2.5% Betaisodona solution) are not genotoxic in the chromosome aberration test. Using the comet assay and chromosome aberration test to characterise the genotoxic potency of povidone-iodine within 4 h of contact with CHO-K1 cells, there was no chromosome damage detected.
Institute of Hygiene and Environmental Medicine, Ernst Moritz Arndt University, Greifswald, Germany.
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G
Müller
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DN
Hai
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A
Kramer
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000633:toxicity*; D016466:CHO Cells / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D008081:Liposomes; D009152:Mutagenicity Tests; D009824:Ointments; D019999:Pharmaceutical Solutions; D011206:Povidone-Iodine / Q000008:administration & dosage / Q000633:toxicity*
D000891:Anti-Infective Agents, Local; D008081:Liposomes; D009824:Ointments; D019999:Pharmaceutical Solutions; D011206:Povidone-Iodine