Formation of human prostate tissue from embryonic stem cells.
Rodent models and immortalized or genetically modified cell lines are frequently used-but have limited utility-for studying human prostate development and maturation. Using rodent mesenchyme to establish reciprocal mesenchymal-epithelial cell interactions with human embryonic stem cells (hESCs), we generated human prostate tissue expressing prostate-specific antigen (PSA) within 8-12 weeks. This human prostate model shows species-conserved signalling mechanisms that could extend to integumental, gastrointestinal and genital tissues.
Centre for Urological Research, Monash Institute of Medical Research, Monash University, 27-31 Wright Street, Clayton, Victoria, Australia 3168.
Renea A
RA
Taylor
Prue A
PA
Cowin
Gerald R
GR
Cunha
Martin
M
Pera
Alan O
AO
Trounson
John
J
Pedersen
Gail P
GP
Risbridger
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D008297:Male; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D011467:Prostate / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D017430:Prostate-Specific Antigen / Q000096:biosynthesis*; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D046509:Tissue Culture Techniques
D017430:Prostate-Specific Antigen