Preparation and characterization of monoclonal antibody against protein TREM-like transcript-1 (TLT-1).
TREM-like transcript-1 (TLT-1) was initially characterized as the putative inhibitory receptor within the TREM locus on chromosome 6 and was suggested to play a critical role in modulating TREM-associated immunity. Recently, TLT-1 was found not to function as an inhibiting member of the TREM family; however, it may play a significant role in maintaining vascular hemostasis, coagulation, and inflammation at sites of injury through its location in platelets. In this study, we have successfully generated a monoclonal antibody that can be applied to assays, including enzyme-linked immunosorbent assay (ELISA), Western immunoblotting, immunofluorescence, and Fluorescence-Activated Cell Sorter (Becton-Dickinson) (FACS), for the study of TLT-1 functions in physiological and pathological conditions.
Lu
Yen-Ta
YT
Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan.
Yen
Chia-Ying
CY
Ho
Hsin-Chun
HC
Chen
Chia-Ju
CJ
Wu
Ming-Fang
MF
Hsieh
Shie-Liang
SL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D001792:Blood Platelets / Q000378:metabolism; D002459:Cell Fusion; D002478:Cells, Cultured; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007118:Immunoassay / Q000379:methods; D051379:Mice; D009928:Organ Specificity; D011971:Receptors, Immunologic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; C473366:TREML1 protein, human