[Chemical constituents from tuber of Cremastra appendiculata].
OBJECTIVE
To investigate the chemical constituents from the tuber of the planted Cremastra appendiculata.


METHODS
The compounds were isolated by column chromatography over silica gel, Sephadex LH-20 and RP-HPLC, and their structures were elucidated on the basis of spectroscopic analysis.


RESULTS
Eight compounds were isolated, and identified as cirrhopetalanthrin (I), 7-hydroxy-4-methoxyphenanthrene-2-O-beta-D-glucoside (II), 4-(2-hydroxyethyl)-2-methoxyphenyl-1-O-beta-D-glucopyranoside (III), tyrosol 8-O-beta-D-gluco-pyranoside (IV), vanilloloside (V), p-hydroxybenzaldehyde (VI), sucrose (VII), adenosine (VIII).


CONCLUSIONS
These compounds are isolated from this plant for the first time. All compounds were evaluated against human colon cancer (HCT-8), human hepatoma (Bel7402), human stomach cancer(BGC-823), human lung adenocarcinoma (A549), human breast cancer (MCF-7), and human ovarian cancer (A2780) cell lines, and cirrhopetalanthrin (I) showed non-selective moderate cytotoxicity with IC50 values of 8.4-13.3 micromol x L(-1), and other compounds were inactive.
Xia
Wen-bin
WB
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.
Xue
Zhen
Z
Li
Shuai
S
Wang
Su-juan
SJ
Yang
Yong-chun
YC
He
Ding-xiang
DX
Ran
Guang-lun
GL
Kong
Ling-zhong
LZ
Shi
Jian-gong
JG
D000241:Adenosine / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001547:Benzaldehydes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D029595:Orchidaceae / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013395:Sucrose / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D001547:Benzaldehydes; D013395:Sucrose; D000241:Adenosine; C011483:4-hydroxybenzaldehyde