Multiplex analysis of expression of three IFNbeta-induced genes in antibody-positive MS patients.
Some interferon beta (IFNbeta)-treated patients with multiple sclerosis develop antibody-mediated decreased bioactivity with resultant loss of therapeutic effect. The authors developed real-time multiplex reverse transcriptase PCR to measure expression of three IFNbeta-inducible genes to directly assess IFNbeta bioactivity in patients with neutralizing antibodies (NAbs). The three genes responded in tandem. Correlation of NAb level with bioactivity at low/moderate NAb levels was poor, indicating that for such patients, direct measurement of IFNbeta bioactivity is most reliable.
Pachner
Andrew R
AR
University of Medicine and Dentistry of New Jersey, Medical School, Newark, NJ 07103, USA. pachner@umdnj.edu
Narayan
Kavitha
K
Pak
Elena
E
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics; D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D016022:Case-Control Studies; D019204:GTP-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D016899:Interferon-beta / Q000627:therapeutic use*; D009103:Multiple Sclerosis / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology*; D064547:Myxovirus Resistance Proteins; D009500:Neutralization Tests; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011506:Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000090123:Viperin Protein
D015088:2',5'-Oligoadenylate Synthetase; D000276:Adjuvants, Immunologic; D019204:GTP-Binding Proteins; D016899:Interferon-beta; D064547:Myxovirus Resistance Proteins; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011506:Proteins; C444427:RSAD2 protein, human; D000090123:Viperin Protein