[Separation and culture of mouse embryonic palatal mesenchymal cells in vitro].
OBJECTIVE
To modify the operation of dissecting embryonic palatal shelves and purify the mouse embryonic palatal mesenchymal (EPM) cells in primary culture.


METHODS
The embryonic palatal shelves were dissected using a surgical microscope by modified operation. Then the embryonic palatal shelves were incubated with Dispase and the isolated EPM cells were cultured. Immunofluorescence technique was used to identify the characteristics of cells.


RESULTS
Embryonic palatal shelves could be dissected accurately and easily with a modified operation. The purified EPM cells contained scarcely epithelial cells. EPM cells were anti-HNK-1, S-100, vimentin positive and anti-CK negative.


CONCLUSIONS
A modified method for dissecting embryonic palatal shelves and purifying the EPM cells of primary culture was established.
Xiao
Wen-lin
WL
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Shi
Bing
B
Huang
Lei
L
Zheng
Qian
Q
Li
Sheng
S
Lu
Yong
Y
D000818:Animals; D018959:CD57 Antigens / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010159:Palate / Q000166:cytology* / Q000196:embryology; D009418:S100 Proteins / Q000032:analysis; D014746:Vimentin / Q000032:analysis
D018959:CD57 Antigens; D009418:S100 Proteins; D014746:Vimentin