Monad, a WD40 repeat protein, promotes apoptosis induced by TNF-alpha.
WD40 repeat proteins have a wide range of diverse biological functions including signal transduction, cell cycle regulation, RNA splicing, and transcription. Here we report the identification and characterization of a novel human WD40 repeat protein, Monad. Monad is unique, since it contains only two WD40 repeats. Monad is widely expressed in human tissues with the highest expression in testis. Overexpression of Monad in HEK293 cells potentiated apoptosis and caspase-3 activation induced by tumor necrosis factor-alpha and cycloheximide. These results raise the possibility that Monad may function as a novel modulator of apoptosis pathway.
Saeki
Makio
M
Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan.
Irie
Yasuyuki
Y
Ni
Lin
L
Yoshida
Miki
M
Itsuki
Yuki
Y
Kamisaki
Yoshinori
Y
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000502:physiology*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D008297:Male; D051379:Mice; D008840:Microfilament Proteins / Q000502:physiology*; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D020449:Repetitive Sequences, Amino Acid; D016415:Sequence Alignment; D013737:Testis / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D051017:Apoptosis Regulatory Proteins; C509833:DNAAF10 protein, human; D008840:Microfilament Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C118067:WDR1 protein, human; C505407:CASP3 protein, human; C505409:Casp3 protein, mouse; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases