"In vitro" effect of lipid peroxidation metabolites on elongation factor-2.
Elongation Factor-2 (eEF-2) is the protein that catalyzes the translocation of the ribosome through mRNA. Not all oxidants affect eEF-2, which is extremely sensitive to oxidative stress caused mainly by lipid peroxidant compounds such as cumene hydroperoxide and t-butyl hydroperoxide. Lipid peroxides constitute a potential hazard to living organisms because of their direct reactivity with a variety of biomolecules and the ability to decompose into free radicals and reactive aldehydes. In this "in vitro" study, we show the effect of three of these aldehydes on the levels of hepatic eEF-2. The results suggest that the toxicity associated with prooxidant-mediated hepatic lipid peroxidation on protein synthesis can originate from the interaction of the aldehydic end products of lipid peroxidation with eEF-2.
Departamento de Bioquímica, Bromatología, Toxicología, Facultad de Farmacia, Universidad de Sevilla, C/.Tramontana s/n, 41012-Sevilla, Spain.
Sandro
S
Argüelles
Alberto
A
Machado
Antonio
A
Ayala
D000079:Acetaldehyde / Q000494:pharmacology; D000447:Aldehydes / Q000494:pharmacology; D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology; D007659:Ketones / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000494:pharmacology; D018384:Oxidative Stress / Q000502:physiology; D020652:Peptide Elongation Factor 2 / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D000447:Aldehydes; D001555:Benzene Derivatives; D007659:Ketones; D020652:Peptide Elongation Factor 2; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; D000079:Acetaldehyde; C027576:4-hydroxy-2-nonenal; C007164:cumene hydroperoxide