Determination of amino acids in saliva using capillary electrophoresis with fluorimetric detection.
In the present study a sensitive method for the quantification of main free amino acids in saliva using capillary electrophoresis with laser induced fluorescence detection was developed. As background electrolyte 20 mM borate buffer pH 9.5 was used. Amino acids were separated after derivatization with fluorescein isothiocyanate (FITC) and the conditions for derivatization were optimized. The main amino acids occurring in saliva (Pro, Ser, Gly and Glu) were separated in less than 7 min. The parameters of validation such as linearity of response, precision and detection limits were determined. The detection limits were obtained in the range from 0.1 to 2.4 nM. The developed method was employed for determination of amino acids in real saliva samples.
Pobozy
Ewa
E
Departament of Chemistry, Warsaw University, Pasteura 1, 02-093 Warsaw, Poland. ewapob@chem.uw.edu.pl
Czarkowska
Wioletta
W
Trojanowicz
Marek
M
D000596:Amino Acids / Q000032:analysis*; D019075:Electrophoresis, Capillary; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005470:Fluorometry; D006801:Humans; D012463:Saliva / Q000737:chemistry*
D000596:Amino Acids; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate