Assessment of Rab11-FIP2 interacting proteins in vitro.
Members of the Rab family of small GTPases are involved in multiple trafficking events in both endocytotic and biosynthetic pathways. To understand more fully the regulation of these events, a concerted effort is underway to ascertain the binding partners and regulators of Rabs. Here, we describe methods to assess binding of Rab11a with Rab11-FIP2 and other Rab11-FIPs utilizing a modified far-Western approach. We then broaden this application to assess binding of Rab11-FIP2 with myosin Vb and homodimerization of Rab11-FIP2.
Ducharme
Nicole A
NA
Jin
Min
M
Lapierre
Lynne A
LA
Goldenring
James R
JR
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000378:metabolism*; D018995:Myosin Heavy Chains; D024701:Myosin Type V; D009218:Myosins / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D020691:rab GTP-Binding Proteins
D002352:Carrier Proteins; C505832:MYO5B protein, human; D008565:Membrane Proteins; D011994:Recombinant Proteins; D024701:Myosin Type V; D018995:Myosin Heavy Chains; D009218:Myosins; C463963:RAB11FIP2 protein, human; D020691:rab GTP-Binding Proteins