[Effect of soluble granule of Shengjing on the apoptosis in spermatogenic cells of rats caused by varicocele].
OBJECTIVE
To investigate the apoptosis of spermatogenic cells of rats caused by experimental varicocele and effect of soluble granules of Shengjing on the apoptosis.


METHODS
Sixty Wistar male rats were divided into three group: control group, model group, and treatment group. Experimental varicocele was induced by partial ligation of the left renal vein in Wistar rat. Apoptosis cells in the testes were detected by in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) Technique.


RESULTS
The apoptosis index in model group was remarkably higher than that in control group (P < 0.001). There was significant difference between treatment and model groups in apoptosis index.


CONCLUSIONS
The apoptosis of spermatogenic cells by varicocele may play a important role in leading to subfertility. The soluble granules of Shengjing have negative effects on the apoptosis of spermatogenic cells. The medicine can not only protect spermic production but improve the ability of procreation of testes.
Zhou
Chuan-ye
CY
Department of Urology, No. 455 Hospital of PLA, Shanghai 200052, China.
An
Li-wen
LW
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007248:Infertility, Male / Q000188:drug therapy / Q000209:etiology; D008297:Male; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology*; D014646:Varicocele / Q000150:complications / Q000188:drug therapy / Q000473:pathology*
D004365:Drugs, Chinese Herbal