Western blotting.
Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly. The scientific community is now confronted with a variety of ways and means to carry out this transfer. This review describes the various procedures that have been used to transfer proteins from a gel to a membrane based on the principles of simple diffusion, vacuum-assisted solvent flow and electrophoretic elution. Finally, a brief description of methods generally used to detect antigens on blots is also described.
Kurien
Biji T
BT
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA. biji-kurien@omrf.ouhsc.edu
Scofield
R Hal
RH
D000818:Animals; D000941:Antigens / Q000737:chemistry; D019921:Biotinylation; D015153:Blotting, Western / Q000295:instrumentation* / Q000379:methods*; D003101:Collodion / Q000737:chemistry; D004058:Diffusion; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D008567:Membranes, Artificial; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012997:Solvents
D000941:Antigens; D008567:Membranes, Artificial; D011506:Proteins; D012997:Solvents; D003101:Collodion