Fluorescent microscopy-based assays to study the role of Rab22a in clathrin-independent endocytosis.
The endocytic and trafficking route followed by proteins that enter cells independently of clathrin is not well understood. In HeLa cells, a distinct endocytic and recycling pathway is followed for such clathrin-independent cargo proteins, and we have characterized the role of Rab proteins in this process. Here we describe cell-based flourescence assays to examine the effects of expression and depletion of Rab22 on endosomal morphology and endocytic recycling.
Roberto
R
Weigert
Julie G
JG
Donaldson
D001483:Base Sequence; D002966:Clathrin / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008285:Major Histocompatibility Complex; D008856:Microscopy, Fluorescence / Q000379:methods*; D034741:RNA, Small Interfering; D014168:Transferrin / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000502:physiology*
D002966:Clathrin; C484191:RAB22A protein, human; D034741:RNA, Small Interfering; D014168:Transferrin; D049452:Green Fluorescent Proteins; D020691:rab GTP-Binding Proteins