Production of biogenic amines by lactic acid bacteria: screening by PCR, thin-layer chromatography, and high-performance liquid chromatography of strains isolated from wine and must.
Biogenic amines are frequently found in wine and other fermented food. We investigated the ability of 133 strains of lactic acid bacteria isolated from musts and wines of different origins to produce histamine, tyramine, and putrescine. We detected the genes responsible for encoding the corresponding amino acid decarboxylases through PCR assays using two primer sets for every gene: histidine decarboxylase (hdc), tyrosine decarboxylase (tdc), and ornithine decarboxylase (odc); these primers were taken from the literature or designed by us. Only one strain of Lactobacillus hilgardii was shown to possess the hdc gene, whereas four strains of Lactobacillus brevis had the tdc gene. None of the Oenococcus oeni strains, the main agents of malolactic fermentation, was a biogenic amine producer. All PCR amplicon band-positive results were confirmed by thin-layer chromatography and high-performance liquid chromatography analyses.
CRA-Istituto Sperimentale per l'Enologia, Asti, Italy.
Antonella
A
Costantini
Manuela
M
Cersosimo
Vincenzo
V
Del Prete
Emilia
E
Garcia-Moruno
D001483:Base Sequence; D001679:Biogenic Amines / Q000032:analysis / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005285:Fermentation; D005516:Food Microbiology; D006632:Histamine / Q000032:analysis / Q000096:biosynthesis; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011700:Putrescine / Q000032:analysis / Q000096:biosynthesis; D016415:Sequence Alignment; D014439:Tyramine / Q000032:analysis / Q000096:biosynthesis; D027843:Vitis / Q000382:microbiology*; D014920:Wine / Q000382:microbiology*
D001679:Biogenic Amines; D006632:Histamine; D011700:Putrescine; D014439:Tyramine