Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation.
The changes in synaptic morphology and receptor content that underlie neural plasticity are poorly understood. Here, we use a pH-sensitive green fluorescent protein to tag recombinant glutamate receptors and monitor their dynamics onto dendritic spine surfaces. We show that chemically induced long-term potentiation (chemLTP) drives robust exocytosis of AMPA receptors. In contrast, the same stimulus produces a small reduction of NMDA receptors from the spine surface. chemLTP produces similar modification of small and large spines. Interestingly, during chemLTP induction, spines increase in volume before accumulation of AMPA receptors on their surface, indicating that distinct mechanisms underlie changes in morphology and receptor content.
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Charles D
CD
Kopec
Bo
B
Li
Wei
W
Wei
Jannic
J
Boehm
Roberto
R
Malinow
D000818:Animals; D005089:Exocytosis / Q000502:physiology*; D006624:Hippocampus / Q000502:physiology*; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D018091:Receptors, AMPA / Q000502:physiology; D017470:Receptors, Glutamate / Q000502:physiology*; D013131:Spine / Q000502:physiology*; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D014162:Transfection
D018091:Receptors, AMPA; D017470:Receptors, Glutamate