[Study on the preparation and bio-distribution of silybin lipid nanospheres].
OBJECTIVE
To prepare silybin lipid nanospheres (SLN) and to evaluate the properties of morphology, particle size and the silybin distribution in mice.


METHODS
Silybin lipid nanospheres were prepared by thin film emulsion-high pressure homogenization technique. Concentrations of silybin in mice blood, liver, spleen, lung, kidney, brain, heart, stomach after oral administration were determined by high performance liquid chromatography.


RESULTS
Scanning electron micrograph showed that most of the SLN were spherical. The average diameter from photon correlation spectrometer was 148.9 nm with the polydispersity 0.17. Body distribution data indicated that SLN could increase the distribution of silybin in blood and liver, decrease the amount of silybin in stomach as compared with the preparation on market, and the drug targeting index (DTI) in liver was 1.81.


CONCLUSIONS
SLN can increase the uptake of silybin in liver after oral administration, which must benefit the hepatitis treatment.
School of Pharmacy, Jiangsu University, Zhenjiang 212001, China.
Xi-Ming
XM
Xu
Qiang
Q
Li
Yuan
Y
Zhu
Song
S
Shen
Zhu
Z
Shen
Jiang-Nan
JN
Yu
D000284:Administration, Oral; D000818:Animals; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D016503:Drug Delivery Systems; D008099:Liver / Q000378:metabolism*; D051379:Mice; D053758:Nanoparticles; D010316:Particle Size; D010743:Phospholipids; D011897:Random Allocation; D000077385:Silybin; D012838:Silymarin / Q000008:administration & dosage / Q000493:pharmacokinetics; D014018:Tissue Distribution
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D010743:Phospholipids; D012838:Silymarin; D000077385:Silybin