Enhanced repair of DNA interstrand crosslinks in S phase.
Hela cells synchronized in G1 and S phases of the cell cycle were transfected with pEGFP crosslinked with trioxsalen. Twelve hours later the number of fluorescent cells was determined by fluorescent microscopy. Cells in S phase have repaired 0.2-0.3 ICL/kb over the 12h period, while cells in G1 phase repaired interstrand crosslinks much more poorly. The crosslinked plasmids were efficiently recruited to the nuclear matrix both in G1 phase and S-phase, which showed that the poor repair of G1 cells was a result of a lack of DNA replication rather than of a lack of matrix attachment.
Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Block 21, 1113 Sofia, Bulgaria.
Veronika
V
Mladenova
George
G
Russev
D003432:Cross-Linking Reagents / Q000737:chemistry; D018736:DNA Adducts / Q000032:analysis; D004260:DNA Repair; D004261:DNA Replication; D016193:G1 Phase / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D016196:S Phase / Q000235:genetics*; D014307:Trioxsalen / Q000737:chemistry
D003432:Cross-Linking Reagents; D018736:DNA Adducts; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D014307:Trioxsalen