SDS-PAGE and Western blotting to detect proteins and glycoproteins of interest in breast cancer research.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting to detect proteins and glycoproteins is one of the most widely used and broadly useful techniques in cancer research, allowing the proteins in a complex sample--such as a blood sample, aspirate, or solid tumor homogenate--to be separated according to molecular weight and visualized within a gel matrix and/or, once separated, transferred onto a supporting membrane, where they may be probed for the binding of antibodies or lectins. In this chapter, the theory and principles of SDS-PAGE and Western blotting are briefly outlined, and basic methods are given that can be applied to investigate virtually any (glyco)protein of interest in breast cancer research.
Department of Physiology, University of Birmingham Medical School, UK.
Chloe
C
Osborne
Susan A
SA
Brooks
D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western / Q000379:methods*; D001943:Breast Neoplasms / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*
D014408:Biomarkers, Tumor; D006023:Glycoproteins; D009363:Neoplasm Proteins