Expression of Nm23 in gliomas and its effect on migration and invasion in vitro.
BACKGROUND
Despite the well-documented importance of Nm23 in the control of metastasis, there is currently a paucity of data regarding the role of this gene family in the control of glioma invasion.


METHODS
Nm23-H1 expression in gliomas was assessed via immunohistochemistry, Western blot, RT-PCR and Northern blot analyses. The migration and invasion ability were also investigated in primary glioma culture cells, human glioma cell lines and nm23-H1 transfectant, using an in vitro brain slice invasion model and a simple scratch technique.


RESULTS
Although no significant correlations were detected between nm23-H1 expression and pathological grade, the endogenous nm23-H1 expression in gliomas was found to be inversely correlated with their migratory abilities. Additionally, the nm23-H1 transfectant resulted in a reduction of approximately 45% of the migratory ability and suppressed the invasiveness of the parental cell line.


CONCLUSIONS
Our overall findings suggest that nm23-H1 may play an important role in the suppression of glioma invasion and migration.
Department of Neurosurgery, Chonnam National University Hospital and Medical School, Gwangiu, South Korea. sjung@chonnam.ac.kr
Shin
S
Jung
Yun-Woong
YW
Paek
Kyung-Sub
KS
Moon
Sung-Chan
SC
Wee
Hyang-Hwa
HH
Ryu
Young-Il
YI
Jeong
Heung-Suk
HS
Sun
Yong-Hao
YH
Jin
Kyung-Keun
KK
Kim
Kyu-Youn
KY
Ahn
D001254:Astrocytoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D054778:NM23 Nucleoside Diphosphate Kinases; D009361:Neoplasm Invasiveness; D009701:Nucleoside-Diphosphate Kinase / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
D054778:NM23 Nucleoside Diphosphate Kinases; D049452:Green Fluorescent Proteins; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase