[Parallel catalytic hydrogen wave of acetylspiramycin caused by dissolved oxygen and its application].
OBJECTIVE
To propose a novel polarographic method for the determination of acetylspiramycin (ASPM) is proposed.


METHODS
In 0.1 mol x L(-1) NH4Cl-NH3 x H2O (pH 8.9) buffer containing dissolved oxygen, ASPM yielded a sensitive parallel catalytic hydrogen wave with the peak potential of -1.63 V (vs SCE) by single sweep polarography.


RESULTS
The 2nd order derivative peak currents (i(p)") of the parallel catalytic hydrogen waves of ASPM showed a linear relationship with its concentrations in the range from 1.74 x 10(-3) microg x mL(-1) to 3.84 microg x mL(-1) (r = 0.9979, n=13). Its detection limit was 5.80 x l0(-4) microg x mL(-1) (3sigma) and RSD (n=13) was 1.24% at the concentration level of 0.871 microg x mL(-1).


CONCLUSIONS
The proposed method could be applied to the determination of ASPM in ASPM tablets.
Department of Chemistry and Chemical Engineering, Weinan Normal College, Weinan 714000, China. wnwfm@wntc.edu.cn
Fu-min
FM
Wang
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis*; D002384:Catalysis; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000737:chemistry*; D011048:Polarography / Q000379:methods*; D015203:Reproducibility of Results; D015572:Spiramycin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis; D013607:Tablets
D000900:Anti-Bacterial Agents; D013607:Tablets; C033163:acetylspiramycin; D006859:Hydrogen; D015572:Spiramycin; D010100:Oxygen