Use of streptomycin for precipitation and detection of proteinase K resistant prion protein (PrPsc) in biological samples.
The ability of streptomycin to form multimolecular aggregates with pathogenic prion proteins and their recovery by precipitation via a low-speed centrifugation step has been demonstrated; these novel properties of streptomycin make it a useful substance that increases the sensitivity of laboratory diagnostic techniques for prion infections in man and animals.
Moussa
Aly
A
Agence Française de Sécurité Sanitaire des Aliments, Site de Lyon F69364, France. moussa69@club-internet.fr
Coleman
Anthony W
AW
Bencsik
Anna
A
Leclere
Edwige
E
Perret
Florent
F
Martin
Ambroise
A
Perron
Hervé
H
D000818:Animals; D002417:Cattle; D011232:Chemical Precipitation; D019286:Endopeptidase K / Q000378:metabolism*; D015394:Molecular Structure; D018620:PrPSc Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D012995:Solubility; D013307:Streptomycin / Q000737:chemistry*
D018620:PrPSc Proteins; D019286:Endopeptidase K; D013307:Streptomycin