Transient development of filamentous Thiothrix species in a marine sulfide oxidizing, denitrifying fluidized bed reactor.
In this study, microscopic and molecular microbial analyses were integrated to characterize rapidly developing white filamentous tufts in a fluidized bed reactor used for nitrate removal from a marine recirculating fish culture system. Formation and rapid elongation of the tufts (often exceeding 50 mm day (-1)) was strongly correlated to transient elevated sulfide concentrations (>50 microM) in the reactor. The dominant bacterial constituents of these tufts were filamentous gram-negative bacteria with densely packed intracellular sulfur granules. Using 16S rRNA gene analysis and fluorescence in situ hybridization it was found that these filamentous bacteria represented a novel Thiothrix phylotype closely related (97% sequence identity) to a previously identified Thiothrix strain endogenous to the marine crustacean Urothoe poseidonis. In addition to filamentous morphotypes, rosette-shaped morphotypes of Thiothrix were also detectable within the tufts.
Cytryn
Eddie
E
Department of Animal Sciences, The Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel.
Minz
Dror
D
Gieseke
Armin
A
van Rijn
Jaap
J
D001483:Base Sequence; D019149:Bioreactors / Q000382:microbiology*; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007220:Industrial Waste; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D017422:Sequence Analysis, DNA; D013440:Sulfides / Q000032:analysis / Q000378:metabolism; D053525:Thiothrix / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D014871:Water Microbiology
D017931:DNA Primers; D015342:DNA Probes; D004269:DNA, Bacterial; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; D009584:Nitrogen