Kinase-catalyzed modification of gold nanoparticles: a new approach to colorimetric kinase activity screening.
Peptide-stabilized gold nanoparticles have been enzymatically biotinylated by a kinase-catalyzed reaction using biotin-ATP as a cosubstrate. Upon mixing with avidin-modified particles, solutions of biotinylated particles change color from red to blue, indicating aggregation of particles. On the basis of this reaction, we have developed a simple colorimetric test to monitor kinase inhibitor activity.
Wang
Zhenxin
Z
Centre for Nanoscale Science, Department of Chemistry and School of Biological Sciences, The University of Liverpool, Liverpool L69 7ZD, U.K.
Lévy
Raphaël
R
Fernig
David G
DG
Brust
Mathias
M
D001360:Avidin / Q000737:chemistry / Q000378:metabolism; D019921:Biotinylation; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D003124:Colorimetry / Q000379:methods*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors / Q000032:analysis* / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides; D047428:Protein Kinase Inhibitors; D001360:Avidin; D006046:Gold; D017868:Cyclic AMP-Dependent Protein Kinases; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases