[Study on the new use of antitumor of Dracaena cambodiana].
OBJECTIVE
To screen the antitumor extract from Dracaena cambodiana Pierre ex Gagnep.


METHODS
The 95% ehanol extract from Dracaena cambodiana was treated in the way of solvent partition and resin column chromatography to afford different extracts. The antitumor activities of the different extracts in vitro were determined by MTT assay.


RESULTS
The chloroform extract, ethyl acetate extract, and n-butanol extract showed strong growth inhibitory on B16 cells and SMMC-721 cells,and the extract of methanol elute showed weak activity,whereas the extracts of water,water elution,and 50% methanol/H2O elution had no activity. The chloroform extract was the most active.


CONCLUSIONS
The chloroform extract is the principal antitumor extract from Dracaena cambodiana.
Mei
Wenli
W
State Key Laboratory of Tropical Crops Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou 571101.
Dai
Haofu
H
Wu
Jiao
J
Zhuang
Ling
L
Hong
Kui
K
D000085:Acetates; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002725:Chloroform; D004305:Dose-Response Relationship, Drug; D031409:Dracaena / Q000737:chemistry*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D000085:Acetates; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; C007650:ethyl acetate; D002725:Chloroform