Effects of orally administered Lactobacillus casei DN-114 001 on the composition or activities of the dominant faecal microbiota in healthy humans.
The composition and activities of the faecal microbiota in twelve healthy subjects analysed in a single open study were monitored before (1-week baseline step), during (10 d supplementation step) and after (10 d follow-up step) the ingestion of a fermented milk containing Lactobacillus casei DN-114 001. Fluorescent in situ hybridisation with group-specific DNA probes, real-time PCR using L. paracasei group-specific primers and temporal temperature gradient gel electrophoresis (TTGE) using group-specific primers were carried out, together with bacterial enzyme activity and metabolite analyses to monitor the structure and activities of the faecal microbiota. L. casei DNA was detected in the faeces of all of the subjects by TTGE after 10 d supplementation. Its quantification by real-time PCR showed a 1000-fold increase during the test step compared with initial levels. No major modification in either the dominant members of the faecal microbiota or their activities was observed during the trial. In conclusion, the short-term consumption of a milk product containing L. casei DN-114 001 was accompanied by a high, transient increase in the quantity of this strain in the faeces of all of the subjects without markedly affecting biochemical or bacteriological factors.
Institut National de la Recherche Agronomique, Unité d'Ecologie et Physiologie du Système Digestif, Bâtiment 405, Domaine de Vilvert, 78 352 Jouy en Josas Cedex, France. rochet@jouy.inra.fr
Violaine
V
Rochet
Lionel
L
Rigottier-Gois
Malène
M
Sutren
Marie-Noëlle
MN
Krementscki
Claude
C
Andrieux
Jean-Pierre
JP
Furet
Patrick
P
Tailliez
Florence
F
Levenez
Agnès
A
Mogenet
Jean-Louis
JL
Bresson
Séverine
S
Méance
Chantal
C
Cayuela
Antony
A
Leplingard
Joël
J
Doré
D000284:Administration, Oral; D000328:Adult; D043302:Cultured Milk Products; D004269:DNA, Bacterial / Q000032:analysis; D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D005260:Female; D006801:Humans; D007780:Lacticaseibacillus casei / Q000201:enzymology / Q000302:isolation & purification; D008297:Male; D019936:Probiotics / Q000008:administration & dosage*
D004269:DNA, Bacterial