Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs.
MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. By using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target messenger RNA molecules (mRNAs). Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.
Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA. giraldez@mcb.harvard.edu
Antonio J
AJ
Giraldez
Yuichiro
Y
Mishima
Jason
J
Rihel
Russell J
RJ
Grocock
Stijn
S
Van Dongen
Kunio
K
Inoue
Anton J
AJ
Enright
Alexander F
AF
Schier
D020413:3' Untranslated Regions; D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000502:physiology*; D047108:Embryonic Development; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000502:physiology*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D011108:Polymers / Q000378:metabolism*; D014176:Protein Biosynthesis; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D015854:Up-Regulation; D015027:Zebrafish / Q000196:embryology; D015053:Zygote / Q000502:physiology
D020413:3' Untranslated Regions; D035683:MicroRNAs; D011108:Polymers; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; C069424:polyadenosine; D043244:Ribonuclease III; D000241:Adenosine