[Determination of molecular weight and content of Ginkgo biloba exocarp polysaccharides by HPLC].
OBJECTIVE
To determine the molecular weight and content of Ginkgo biloba exocarp polysaccharides.


METHODS
The analysis was carried on a PL aquagel-OH MIXED (7.5 mm x 300 mm, 8 microm) chromatography column eluted with water as mobile phase at 1.0 mL x min(-1) of flow rate, the column temperature was 25 degrees C and the eluate was detected by RID.


RESULTS
The average molecular weight of Ginkgo bilobaexocarp polysaccharides was 11 062.5 with RSD = 0.78% (n = 6); the content was 81.9% with RSD = 2.5% (n = 6), the standard curves of dextran (MW 12 000) were linear in the range of 1-20 microg, r = 0.999 9. The average recovery is 97.9%, RSD was 2.5%.


CONCLUSIONS
This method was found to be sensitive and accurate for the measurement of Ginkgo biloba exocarp polysaccharides.
Medical College of Yangzhou University, Yangzhou 225001, China.
Ping
P
Lu
Li
L
Ren
Ai-hua
AH
Xu
Hua-sheng
HS
Chen
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D020441:Ginkgo biloba / Q000737:chemistry*; D008970:Molecular Weight; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results
D011134:Polysaccharides