The expression of genes for transepithelial calcium-transporting proteins in the bovine duodenum.
Intestinal epithelial cells contain calcium-binding proteins and Ca2+-transporting adenosine triphosphatase (Ca2+-ATPase), which play important roles in intestinal Ca transport. However, the factors that affect the expression of these transepithelial Ca-transporting proteins in dairy cattle are unknown. In this study, a semi-quantitative reverse transcription polymerase chain reaction was used to determine the expression of the mRNAs for intestinal Ca-binding protein calbindin-D9k (CaBP9k), two isoforms of plasma membrane Ca2+-ATPase (PMCA1 and PMCA4), and vitamin D receptor (VDR) in duodenal tissue samples from 20 non-lactating, non-pregnant Holstein dairy cattle (0.4-135.9 months old). The correlations between the expressions of transepithelial Ca-transporting proteins, the ages of the cattle, and the presence of several plasma components were evaluated. The duodenal CaBP9k mRNA content had a significant negative correlation with age and positive correlations with plasma inorganic phosphorus (iP) and 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) concentrations. The PMCA1 mRNA content was negatively correlated with the plasma Ca concentration. The duodenal PMCA4 mRNA content was correlated negatively with the plasma iP. The VDR mRNA content had a positive correlation with the plasma magnesium concentration.
Yamagishi
N
N
Research Centre for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. yamagisi@obihiro.ac.jp
Miyazaki
M
M
Naito
Y
Y
D000818:Animals; D064026:Calbindins; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D000252:Calcium-Transporting ATPases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D002417:Cattle / Q000097:blood / Q000378:metabolism / Q000502:physiology*; D004386:Duodenum / Q000378:metabolism / Q000502:physiology*; D005260:Female; D007413:Intestinal Mucosa / Q000502:physiology; D007527:Isoenzymes; D008274:Magnesium / Q000097:blood; D008297:Male; D010758:Phosphorus / Q000097:blood; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018167:Receptors, Calcitriol / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D014807:Vitamin D / Q000031:analogs & derivatives / Q000097:blood
D064026:Calbindins; D002135:Calcium-Binding Proteins; D007527:Isoenzymes; D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D064030:S100 Calcium Binding Protein G; D014807:Vitamin D; D010758:Phosphorus; C097949:1,25-dihydroxyvitamin D; D000252:Calcium-Transporting ATPases; D008274:Magnesium; D002118:Calcium