Dual tropism of HIV-1 envelopes derived from renal tubular epithelial cells of patients with HIV-associated nephropathy.
The phenotype of HIV-1 gp120 envelope derived from renal epithelium and peripheral blood mononuclear cells (PBMC) of patients with HIV-associated nephropathy was investigated in vitro. Chimeric viruses were derived from kidney or blood and used to infect primary CD4+T cells, cell lines expressing single co-receptors and a renal epithelial cell line HPT-1. HIV-1 variants derived from renal epithelium were dual tropic whereas simultaneously derived viruses from PBMC were R5-tropic. Utilization of alternative co-receptors CCR3, BONZO and BOB, also differed.
Division of Infectious Diseases, Department of Medicine, Mt Sinai School of Medicine, New York, NY, USA.
Bouchra
B
Zerhouni-Layachi
Mohammad
M
Husain
Michael J
MJ
Ross
Daniele
D
Marras
Masaaki
M
Sunamoto
Xinyan
X
Liu
Paul E
PE
Klotman
Mary E
ME
Klotman
D016263:AIDS-Associated Nephropathy / Q000235:genetics / Q000473:pathology / Q000821:virology*; D002460:Cell Line; D046528:Chimerism; D004847:Epithelial Cells / Q000821:virology; D015699:HIV Envelope Protein gp120 / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007684:Kidney Tubules / Q000473:pathology / Q000821:virology; D007963:Leukocytes, Mononuclear / Q000821:virology; D010641:Phenotype; D054397:Receptors, CCR3; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine / Q000378:metabolism; D018121:Receptors, Cytokine / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism; D018523:Tropism / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics*
C515350:CCR3 protein, human; C106330:CXCR6 protein, human; D015699:HIV Envelope Protein gp120; D054397:Receptors, CCR3; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D043562:Receptors, G-Protein-Coupled; D011991:Receptors, Virus