Analysis of hVps34/hVps15 interactions with Rab5 in vivo and in vitro.
The hVps34 phosphatidylinositol (PI) 3-kinase plays an important role in the regulation of vesicular trafficking in the endosomal system. hVps34 associates with a myristylated protein kinase, hVps15. The two proteins are targeting to early endosomal membranes by interactions between hVps15 and activated (GTP-bound) Rab5. This leads to the production of the hVps34 product, PI(3)P, in the endosomal membrane, and subsequent recruitment of FYVE and PX domain-containing effector proteins. This chapter describes the analysis of hVps34/hVps15 interactions with Rab5 in tissue culture cells and in vitro.
James T
JT
Murray
Jonathan M
JM
Backer
D056827:Endosomal Sorting Complexes Required for Transport; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D011485:Protein Binding; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D058571:Vacuolar Sorting Protein VPS15; D020696:rab5 GTP-Binding Proteins / Q000378:metabolism
D056827:Endosomal Sorting Complexes Required for Transport; D019869:Phosphatidylinositol 3-Kinases; D017346:Protein Serine-Threonine Kinases; D058571:Vacuolar Sorting Protein VPS15; D020696:rab5 GTP-Binding Proteins; C546851:PIK3R4 protein, human