[Isolation and characteristic of a moderately halophilic bacterium accumulated ectoine as main compatible solute].
A moderately halophilic bacterium(designated strain I15) was isolated from lawn soil. Based on the analysis of 16S rDNA (GenBank accession number DQ010162), morphology, physiological and biochemical characteristics, strain I15 was identified as Virgibacillus marismortuii. This strain was capable of growing under 0% approximately 25% NaCl, and exhibited an optimum NaCl concentration of 10% and an optimum temperature of 30 degrees C and an optimum pH of 7.5 - 8.0 for its growth, respectively. Under hyperosmotic stress, strain 115 accumulated ectoine as the main compatible solute. Under 15% NaCl conditions the intracellar ectoine can reach to 1.608 mmol/(g x cdw), accounted for 89.6% of the total compatible solutes. The biosynthesis of ectoine was under the control of osmotic, and the accumulated ectoine synthesized intraceilularly can released under hypoosmotic shocks and resynthesis under hyperosmotic shock rapidly.
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China. hejian@njau.edu.cn
Jian
J
He
Ting
T
Wang
Ji-Quan
JQ
Sun
Li-Feng
LF
Gu
Shun-Peng
SP
Li
D000599:Amino Acids, Diamino / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D012988:Soil Microbiology
D000599:Amino Acids, Diamino; C045628:ectoine