dnaJ and gyrB gene sequence relationship among species and strains of genus Streptococcus.
The dnaJ and gyrB nucleotide sequences were determined for members of the genus Streptococcus. The average similarity between the species tested was 76.4% (69.7-100%) for dnaJ and 75.9 (70.1-98.7%) for gyrB. These data indicated that the dnaJ and gyrB genes are more divergent and more discriminatory than the 16S rDNA gene. Furthermore, the variation in the dnaJ nucleotide sequences among the mitis group was greater than that of the gyrB nucleotide sequences, especially between Streptococcus pneumoniae and Streptococcus mitis. Subsequently, the high discrimination power of dnaJ within the mitis group was confirmed. Thus, we conclude that the dnaJ and gyrB genes are efficient alternative targets for the classification of the genus Streptococcus, and that dnaJ is suitable for phylogenetic analysis of closely related Streptococcus strains.
Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
Yoko
Y
Itoh
Yoshiaki
Y
Kawamura
Hiroaki
H
Kasai
Mohammad Monir
MM
Shah
Pham Hong
PH
Nhung
Makiko
M
Yamada
Xiaosong
X
Sun
Takashi
T
Koyana
Masahiro
M
Hayashi
Kiyofumi
K
Ohkusu
Takayuki
T
Ezaki
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D027081:DNA Gyrase / Q000235:genetics*; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D010802:Phylogeny; D013291:Streptococcus / Q000145:classification* / Q000235:genetics*; D013482:Superoxide Dismutase / Q000235:genetics
D001426:Bacterial Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase; D027081:DNA Gyrase