Ovarian response to repeated administration of alternating exogenous gonadotropin regimens in the ocelot (Leopardus pardalis) and tigrinus (Leopardus tigrinus).
Exogenous gonadotropins are used to stimulate ovarian follicular growth and ovulation in mammalian species, including wild cats. However, successes in application of assisted reproduction techniques in nondomestic felids have been sparse. Our objectives were to assess the effectiveness of alternating gonadotropin regimens on ovarian responses. Five adult female ocelots and four adult female tigrinus were treated four to six times, using alternating eCG/hCG and pFSH/pLH at 4-month intervals. Laparoscopies were done to assess follicular development and to collect oocytes from matures follicles. The average number of follicles and corpus luteum (CL) per stimulation was higher in ocelots (7.0 +/- 0.8; mean +/- S.E.M.) than in tigrinus (2.5 +/- 0.4; P < 0.05), but the percentage of mature oocytes did not differ between the two species (mean range, 54-55%). Within species, both gonadotropin regimens were equally effective in inducing follicular growth and oocyte maturation. The total number of ovarian structures and oocyte maturation percentages did not decrease in either species with sequential stimulations. In summary, female ocelots and tigrinus continued to respond to repeated alternating ovarian stimulation protocols. In conclusion, the use of alternating gonadotropin regimens may permit more intensive reproductive management in these endangered cats.
Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade, São Paulo, São Paulo, Brazil. repaz@usp.br
Regina Celia Rodrigues
RC
da Paz
Eduardo Antunes
EA
Dias
Cristina Harumi
CH
Adania
Valquíria Hippólito
VH
Barnabe
Renato Campanarut
RC
Barnabe
D000818:Animals; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D045989:Felidae / Q000502:physiology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D006065:Gonadotropins, Pituitary / Q000008:administration & dosage*; D007986:Luteinizing Hormone / Q000008:administration & dosage; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*
D006063:Chorionic Gonadotropin; D006065:Gonadotropins, Pituitary; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone