A resazurin-based biosensor for organic pollutants.
A new rapid biosensor method employing the dye resazurin as an indicator of bacterial respiration has been developed to provide a rapid, facile and specific biosensor for environmental contaminants that does not rely on genetic modification techniques, is suitable for a high-throughput multiwell format, and is ideally suited to resource-constrained environmental monitoring situations. This whole-cell biosensor has been applied to the test analyte toluene using natural toluene-degrading bacteria as the biological component and is competitive with more complex recombinant approaches. The redox-driven biosensor is dependent on the catabolism of a specific compound, concomitantly reducing the redox indicator resazurin to provide the analytical signal in a whole-cell biosensor assay.
Tizzard
Aynsley C
AC
Lincoln Ventures, Lincoln University, PO Box 133, Lincoln 8152, New Zealand.
Bergsma
Joost H
JH
Lloyd-Jones
Gareth
G
D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D016956:Burkholderia cepacia / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis / Q000494:pharmacology; D009930:Organic Chemicals / Q000032:analysis / Q000494:pharmacology; D010078:Oxazines / Q000032:analysis* / Q000378:metabolism; D011549:Pseudomonas / Q000187:drug effects / Q000378:metabolism*; D014050:Toluene / Q000032:analysis* / Q000494:pharmacology*; D014966:Xanthenes / Q000032:analysis* / Q000378:metabolism
D004785:Environmental Pollutants; D009930:Organic Chemicals; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D014050:Toluene