HuC:Kaede, a useful tool to label neural morphologies in networks in vivo.
A simple and efficient procedure for labeling neurons is a prerequisite for investigating the development of neural networks in zebrafish. To label neurons we used Kaede, a fluorescent protein with a photoconversion property allowing conversion from green to red fluorescence following irradiation with UV or violet light. We established a zebrafish stable transgenic line, Tg(HuC:Kaede), expressing Kaede in neurons under the control of the HuC promoter. This transgenic line was used to label a small number of neurons in the trigeminal ganglion. Also, using embryos injected with the transgene, we labeled peripheral axon arbors of a Rohon-Beard neuron at 4 days postfertilization and observed the dendrite development of a tectal neuron for 3 days. These data indicate that Kaede is a useful tool to selectively label neural networks in zebrafish.
Sato
Tomomi
T
Laboratory for Developmental Gene Regulation, Brain Science Institute, RIKEN, Japan.
Takahoko
Mikako
M
Okamoto
Hitoshi
H
D000818:Animals; D003712:Dendrites / Q000502:physiology; D051959:ELAV Proteins / Q000235:genetics*; D000067778:ELAV-Like Protein 3; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics*; D008845:Microinjections; D009415:Nerve Net / Q000166:cytology / Q000196:embryology*; D009474:Neurons / Q000166:cytology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D019076:Transgenes; D012668:Trigeminal Ganglion / Q000166:cytology / Q000196:embryology*; D015027:Zebrafish / Q000196:embryology; D029961:Zebrafish Proteins / Q000235:genetics*
D051959:ELAV Proteins; D000067778:ELAV-Like Protein 3; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D029961:Zebrafish Proteins; C088635:elavl3 protein, zebrafish