Conformation study of HA(306-318) antigenic peptide of the haemagglutinin influenza virus protein.
Several HLA-DR alleles present the immunodominant HA(306-318) peptide of haemagglutinin of the influenza virus to T cells. NMR data of the peptide in various water solutions exclude any alpha-helix or turn conformations. Circular dichroism and Fourier transform infrared spectroscopies indicate an estimated beta-extended structure in water of 31% and 28%, respectively, with spectra shape similar to the ones observed for beta-sheet containing proteins. The H/D amide exchange suggests a stable length-dependent interchain hydrogen-bonding. The partially beta-extended conformation of HA(306-318) in solution might be close to the one found in HA(306-318)-HLA-DR1 complex. These results suggest different interconverting extended conformations of HA(306-318), depending on the microenvironment of the solution medium. This flexibility emphasizes the ability of some peptides to fit more easily the binding site of several HLA-DR molecules. Similar results were obtained on the HIV P25(263-277) peptide which has been previously shown to be a good DR1 binder. From a vibrational point of view, infrared Amide I frequencies of secondary structures in peptides were ascertained. As previously demonstrated for proteins in solution, Fourier transform infrared and circular dichroism spectroscopies appear to be valuable tools for conformational properties of peptides. Their use may contribute to the detection of peptide conformation-binding relationship which has to be further tested by biochemical and biological studies.
RICMH, INSERM U 395, UPS, IFR 30, CHU Purpan, BP 3028, 31024 Toulouse Cedex, France.
A
A
Bertrand
R M
RM
Brito
A J P
AJ
Alix
J M
JM
Lancelin
R A
RA
Carvalho
C F G C
CF
Geraldes
F
F
Lakhdar-Ghazal
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry*; D002942:Circular Dichroism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry
D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D010446:Peptide Fragments; D012996:Solutions; C086595:influenza hemagglutinin (306-318); D014867:Water