Microemulsion electrokinetic chromatographic determination of bufadienolides in toad venom and in traditional Chinese medicine.
A microemulsion electrokinetic chromatographic (MEEKC) method has been developed and validated for determination of resibufogenin and cinobufagin in toad venom and in traditional Chinese medicine prepared from the venom. The MEEKC method involved use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent, and butan-1-ol as co-solvent. To improve the separation, the effect of temperature and running buffer pH were evaluated. The optimized conditions (heptane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w), and 10 mmol L(-1) sodium tetraborate buffer, pH 9.2, and 298 nm as the detection wavelength) enabled useful and repeatable separation of the analytes.
Xingping
Luo
L
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China. aluo009@yahoo.com.cn
Zongde
Zhai
Z
Yanfang
Zhao
Z
Liren
Chen
C
Yongmin
Li
L
D000664:Amphibian Venoms / Q000737:chemistry*; D000818:Animals; D001001:Anura; D002018:Bufanolides / Q000032:analysis*; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D004655:Emulsions / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008516:Medicine, Chinese Traditional; D008968:Molecular Conformation; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013237:Stereoisomerism; D013696:Temperature; D013997:Time Factors
D000664:Amphibian Venoms; D002018:Bufanolides; D004655:Emulsions; D004364:Pharmaceutical Preparations; D012967:Sodium Dodecyl Sulfate; C005734:bufogenin; C002471:cinobufagin