Protection of the hemopoietic system by Podophyllum hexandrum against gamma radiation-induced damage.
A semi-purified extract of low-altitude Podophyllum hexandrum (REC-2001) containing a relatively low content of podophyllotoxin (3.25 %) exhibited potent antioxidant ability in lipid media (at 1000 microg/mLagainst 0.25 kGy) and significant (p < 0.05) hydroxyl ion scavenging potential (78.83 % at 500 microg/mL). In vitro investigations revealed the ability of REC-2001 to significantly (p < 0.05) reduce radiation-induced hemolysis (2 microg/mL; 46.184 %) and nitric oxide scavenging levels (IC (50): 792 +/- 1.25 microg/mL). Protection of the hemopoietic system of Strain 'A' mice administered 20 mg/kg BW REC-2001 30 min prior to lethal irradiation (10 Gy) was recorded and was mediated by free radical scavenging and lowering of lipid oxidation. Further studies investigating the effects of REC-2001 on stem cell modulation are warranted.
Department of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Brig, SK Mazumdar Road, Delhi-110 054, India.
Ravinder Kumar
RK
Sagar
Raman
R
Chawla
Rajesh
R
Arora
Shikha
S
Singh
Bal
B
Krishna
Rakesh Kumar
RK
Sharma
Satish Chandra
SC
Puri
Pankaj
P
Singh
Raj
R
Kumar
Ashok Kumar
AK
Sharma
Surender
S
Singh
Jagdish
J
Prasad
Vinay
V
Gupta
Bilal
B
Ahmed
Kanaya Lal
KL
Dhar
Haider Ali
HA
Khan
Manju Lata
ML
Gupta
Ghulam Nabi
GN
Qazi
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D028141:Berberidaceae; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D011034:Podophyllotoxin / Q000737:chemistry / Q000302:isolation & purification; D011035:Podophyllum / Q000737:chemistry*; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology*
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D011837:Radiation-Protective Agents; C509544:Sinopodophyllum hexandrum extract; D011034:Podophyllotoxin