Effects of metsulfuron-methyl on the microbial population and enzyme activities in wheat rhizosphere soil.
The effects of metsulfuron-methyl, a sulfonylurea herbicide, on the wheat soil microorganisms were evaluated by the methods of microbial inoculation culture, and the activities of three enzymes were measured using the colorimetric method. The tolerant microorganisms that can resist 500 microg x g(-1) metsulfuron-methyl in the counting culture medium were studied specially. Metsulfuron-methyl distinctly inhibited the common aerobic heterotriphic bacteria, but the effects on common fungi and common actinomycete were not evident. In the meantime, the number of tolerant fungi increased greatly in the rhizosphere after the application of metsulfuron-methyl in contrast to the significant decrease of the amount of tolerant actinomycete. It indicates that fungi might turn into the dominant microbial type and actinomycete is the sensitive factor in the soil polluted by sulfonylurea residues. The population of aromatic compounds-decomposing bacteria, aerobic azotobacter, and nitrite bacteria all increased in the earlier period, but the aerobic azotobacter decreased rapidly in number 30 days later, and the amount of nitrite bacteria also showed a temporary decrease with time 15 days later. However, the denitrifying bacteria just began to increase significantly after the crops had grown for 50 days. The amount of sulfur-oxidizing bacteria gradually decreased with the growth of crops, and so were the sulfate-reducing bacteria after metsulfuron-methyl application. To all types of microorganisms, there were more microbes in rhizosphere samples than those in nonrhizosphere except aerobic azotobacter. It means the growth of wheat root system can stimulate the growth of most microorganisms. The activities of hydrogen peroxidase and polyphenol oxidase in soil samples after metsulfuron-methyl application were notably lower than those in the control, and the difference of the activities between the samples of rhizosphere and nonrhizosphere was evident. On the contrary, the activity of dehydrogenase was not inhibited by the application of metsulfuron-methyl, and the rhizosphere effect was not obvious either.
Department of Environmental Engineering, Huajiachi Campus, Zhejiang University, Hangzhou, Zhejiang, PR China.
Yong Hua
YH
He
Dong Sheng
DS
Shen
Cheng Ran
CR
Fang
Ruo
R
He
Yin Mei
YM
Zhu
D039903:Actinobacteria / Q000187:drug effects / Q000254:growth & development; D001190:Arylsulfonates / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D001420:Bacteria, Aerobic / Q000187:drug effects / Q000254:growth & development; D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000254:growth & development; D004156:Catechol Oxidase / Q000378:metabolism; D003124:Colorimetry; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development; D006540:Herbicides / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D012988:Soil Microbiology; D013997:Time Factors; D014908:Triticum / Q000382:microbiology*
D001190:Arylsulfonates; D006540:Herbicides; C050296:metsulfuron methyl; D004156:Catechol Oxidase; D009195:Peroxidase