Comparative examination of the stability of semiconductor quantum dots in various biochemical buffers.
Due to their greater photostability compared to established organic fluorescence markers, semiconductor quantum dots provide an attractive alternative for the biolabeling of living cells. On the basis of a comparative investigation using differently sized thiol-stabilized CdTe nanocrystals in a variety of commonly used biological buffers, a method is developed to quantify the stability of such a multicomponent system. Above a certain critical size, the intensity of the photoluminescence of the nanocrystals is found to diminish with pseudo-zero-order kinetics, whereas for specific combinations of particle size, ligand, and buffer there appears to be no decay below this critical particle size, pointing out the necessity for thorough investigations of this kind in the view of prospect applications of semiconductor nanocrystals in the area of biolabeling.
Boldt
Klaus
K
Institute of Physical Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
Bruns
Oliver T
OT
Gaponik
Nikolai
N
Eychmüller
Alexander
A
D002021:Buffers; D002104:Cadmium / Q000737:chemistry; D003460:Crystallization; D005456:Fluorescent Dyes / Q000737:chemistry; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D045663:Quantum Dots; D012666:Semiconductors; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013691:Tellurium / Q000737:chemistry
D002021:Buffers; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D002104:Cadmium; D013691:Tellurium