Thermal expansion of blood vessels in low cryogenic temperatures, Part II: Vitrification with VS55, DP6, and 7.05 M DMSO.
As part of the ongoing effort to study the mechanical behavior of biological materials in cryopreservation processes, the current study focuses on thermal expansion during vitrification (vitreous in Latin means glassy). A new device is utilized in this study, which has been described in detail in the companion paper (Part I). The current study (Part II) focuses on measurements of vitrified blood vessels permeated with the cryoprotectants VS55, DP6, and DMSO. Data analysis in this study includes polynomial approximation of experimental results in the lower part of the cryogenic temperature range, where the material behaves as solid over a practical time scale. The study further includes a unified thermal expansion analysis throughout the entire cryogenic temperature range by compiling the current results with previously reported data. Finally, analysis of the glass transition temperature, based on thermal strain data is presented.
Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Jorge L Jimenez
JL
Rios
Yoed
Y
Rabin
D001808:Blood Vessels / Q000502:physiology*; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D005559:Formamides / Q000737:chemistry*; D005615:Freezing; D006531:HEPES / Q000737:chemistry*; D006358:Hot Temperature; D009926:Organ Preservation; D011409:Propylene Glycols / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D003451:Cryoprotective Agents; C481727:DP6 solution; D005559:Formamides; D011409:Propylene Glycols; C481729:VS55 solution; D006531:HEPES; D004121:Dimethyl Sulfoxide