Activation of Rho and Rac by Wnt/frizzled signaling.
Wnt/Frizzled (Fz) signaling controls developmental, physiological, and pathological processes by means of several distinct pathways. Wnt/Fz activation of the small GTPases Rho and Rac has emerged as a key mechanism that regulates cell polarity and movements during vertebrate gastrulation. In this chapter, we describe biochemical assays for the detection of Wnt/Fz activation of Rho and Rac in both mammalian cells and Xenopus embryo explants.
Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Piscataway, USA.
Raymond
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Habas
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D000818:Animals; D051017:Apoptosis Regulatory Proteins; D004789:Enzyme Activation; D051157:Frizzled Receptors / Q000502:physiology*; D019204:GTP-Binding Proteins; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D051153:Wnt Proteins / Q000502:physiology*; D014981:Xenopus / Q000196:embryology; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
D051017:Apoptosis Regulatory Proteins; D051157:Frizzled Receptors; D047908:Intracellular Signaling Peptides and Proteins; C100494:RTKN protein, human; D011993:Recombinant Fusion Proteins; D051153:Wnt Proteins; D005982:Glutathione Transferase; D019204:GTP-Binding Proteins; D020764:cdc42 GTP-Binding Protein; D020744:rac GTP-Binding Proteins; D020741:rho GTP-Binding Proteins