Caspase-3 induced apoptosis in transgenic zebrafish.
Zebrafish is an attractive model organism for studying apoptosis development because of its genetic accessibility. Here we describe the induction of clonally derived apoptosis in transgenic zebrafish expressing mouse caspase-3 (CASP3) under control of the zebrafish beta-actin promoter (betap). Visualization of apoptotic cells, expressing a chimeric transgene encoding CASP3 fused to green fluorescent protein (GFP) gene, revealed that apoptosis arose in the thymus, spread locally into gill arches and retro-orbital soft tissue, and then disseminated into abdominal organs like testis, kidney. This transgenic model provides a platform for over-expression of caspase-3 induced extensive apoptosis in embryos and adult.
Biotechnology Department, Institute of Applied Medicines and Research, Delhi-Meerat Road, 9th Mile Stone, Ghaziabad, UP, India. drchiranjib@yahoo.com
Chiranjib
C
Chakraborty
Gargi
G
Saha
Biplab
B
Sarkar
S
S
Pal
T K
TK
Chatterjee
A K
AK
Sadhu
D000199:Actins / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000002:abnormalities / Q000033:anatomy & histology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000002:abnormalities / Q000033:anatomy & histology* / Q000235:genetics
D000199:Actins; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases