HPLC-DAD/MS characterization of flavonoids and hydroxycinnamic derivatives in turnip tops (Brassica rapa L. Subsp. sylvestris L.).
Flavonoids and hydroxycinnamic derivatives of turnip tops (Brassica rapa L. subsp. sylvestris L.) were characterized for the first time in four samples from different origins. Turnip tops exhibit a high polyphenols content (ranging from 107 to 191 mg/100 g, fresh weight) and a good antiradical activity, determined with the DPPH* test. After a liquid-liquid extraction and fractionation procedures, most flavonoids (isorhamnetin, kaempferol, and quercetin glycosides) and hydroxycinnamic derivatives were identified by means of HPLC-DAD/MS techniques. Isorhamnetin glycosides were the main flavonoid derivatives, differing from that found in the vegetables belonging to the Brassica oleracea group.
Romani
Annalisa
A
Dipartimento di Scienze Farmaceutiche, Università degli Studi di Firenze, via U. Schiff 6, 50019 Sesto Fiorentino, Italy.
Vignolini
Pamela
P
Isolani
Laura
L
Ieri
Francesca
F
Heimler
Daniela
D
D029741:Brassica rapa / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*; D044948:Flavonols / Q000032:analysis; D016166:Free Radical Scavengers / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D013058:Mass Spectrometry; D011794:Quercetin / Q000031:analogs & derivatives; D014675:Vegetables / Q000737:chemistry*
D003373:Coumaric Acids; D005419:Flavonoids; D044948:Flavonols; D016166:Free Radical Scavengers; D006027:Glycosides; C047368:3-methylquercetin; D011794:Quercetin