Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA.
The argonaute protein family provides central components for RNA interference (RNAi) and related phenomena in a wide variety of organisms. Here, we isolated, from a Bombyx mori cell, a cDNA clone named BmAGO2, which is homologous to Drosophila ARGONAUTE2, the gene encoding a repressive factor for the recombination repair of extrachromosomal double-strand breaks (DSBs). RNAi-mediated silencing of the BmAGO2 sequence markedly increased homologous recombination (HR) repair of DSBs in episomal DNA, but had no effect on that in chromosomes. Moreover, we found that RNAi for BmAGO2 enhanced the integration of linearized DNA into a silkworm chromosome via HR. These results suggested that BmAgo2 protein plays an indispensable role in the repression of extrachromosomal DSB repair.
Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Haruna
H
Tsukioka
Masateru
M
Takahashi
Hiroaki
H
Mon
Kazuhiro
K
Okano
Kazuei
K
Mita
Toru
T
Shimada
Jae Man
JM
Lee
Yutaka
Y
Kawaguchi
Katsumi
K
Koga
Takahiro
T
Kusakabe
D000595:Amino Acid Sequence; D000818:Animals; D060565:Argonaute Proteins; D012831:Bombyx / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D002875:Chromosomes / Q000378:metabolism; D003001:Cloning, Molecular; D004249:DNA Damage; D004260:DNA Repair; D029721:Drosophila Proteins / Q000737:chemistry; D020224:Expressed Sequence Tags; D017344:Genes, Insect; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034743:RNA-Induced Silencing Complex / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid
C433807:AGO2 protein, Drosophila; D060565:Argonaute Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D012333:RNA, Messenger; D034743:RNA-Induced Silencing Complex