Development of a 13C-glycocholic acid blood test to assess bacterial metabolic activity of the small intestine in canines.
The objectives of this study were to establish optimal doses of 13C-glycocolic acid (GCA) for use in a GCA blood test as a marker for canine small intestinal bacterial metabolic activity. Four doses of GCA were administered orally to 8 healthy dogs. Blood samples were collected at various time points up to 480 min. The percent dose/min of 13C administered as GCA (PCD) and cumulative PCD (CUMPCD) were determined by fractional mass spectrometry. No dog showed any clinically obvious side effects. Doses of 1 and 2 mg/kg of bodyweight (BW) led to a significant increase in PCD and CUMPCD (P < 0.001). The mean CUMPCD was significantly higher for the 1 mg/kg BW dose compared with the 2 and 4 mg/kg BW doses (P < 0.05). Administration of 1 mg/kg BW of 13C-glycocholic acid led to an increase in CUMPCD over baseline in gas extracted from blood samples and appears to be the best parameter to evaluate for future clinical studies.
Suchodolski
Jan S
JS
Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4474, USA. jsuchodolski@cvm.tamu.edu
Ruaux
Craig G
CG
Steiner
Jörg M
JM
Fetz
Kathrin
K
Berghoff
Nora
N
Williams
David A
DA
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D001424:Bacterial Infections / Q000175:diagnosis / Q000662:veterinary; D015415:Biomarkers / Q000097:blood; D002247:Carbon Isotopes; D002756:Cholagogues and Choleretics / Q000097:blood / Q000493:pharmacokinetics*; D004285:Dogs / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006000:Glycocholic Acid / Q000097:blood / Q000493:pharmacokinetics*; D007421:Intestine, Small / Q000378:metabolism / Q000382:microbiology*; D013058:Mass Spectrometry / Q000662:veterinary
D015415:Biomarkers; D002247:Carbon Isotopes; D002756:Cholagogues and Choleretics; D006000:Glycocholic Acid