[Experimental study of the effects of puerarin on biological characters of osteoblasts in vitro].
OBJECTIVE
To observe the effect of puerarin on cell proliferation, differentiation, maturation and mineralization in cultured rat osteoblasts.


METHODS
Osteoblasts from craniums of newly born SD rats were cultured in vitro. MTT, PNPP and ARS were used to observe the proliferation, activity of ALP and the number of mineral node of cultured osteoblasts in vitro.


RESULTS
It was found that puerarin had the effect on stimulating cell proliferation, activity of ALP and the number of mineral node of cultured osteoblasts (P < 0.01 or P < 0.05).


CONCLUSIONS
Puerarin can promote proliferation, differentiation, maturation and mineralization of the osteoblasts in vitro.
Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. zhm197@163.com
Hong-Min
HM
Zang
Jun-Chang
JC
Chen
Yi-Heng
YH
Liu
Kun-Zheng
KZ
Wang
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D007529:Isoflavones / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000166:cytology
D007529:Isoflavones; D000469:Alkaline Phosphatase; C033607:puerarin