Inhibition of stem elongation in spinach by theobroxide.
In the current study, we investigated the influences of theobroxide on stem elongation in spinach (Spinacia oleracea). Our results showed that stem elongation and flower formation were inhibited by spraying spinach plants with theobroxide under inductive, long day conditions (16 h light/8 h dark), while application of exogenous applied GA3 prevented the effect of theobroxide. Quantitative analysis showed that theobroxide suppressed GA1 biosynthesis, whereas the endogenous content of jasmonic acid was unchanged. However, under short day conditions (10 h light/14 h dark), there were no differences in stem length between treated and untreated plants. These results suggest that the inhibition of stem elongation by theobroxide is probably due to the suppression of gibberellin biosynthesis.
Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kitaku, Sapporo 060-8589, Japan. fjkong@chem.agr.hokudai.ac.jp
Fanjiang
F
Kong
Xiquan
X
Gao
Kyong-Hee
KH
Nam
Kosaku
K
Takahashi
Hideyuki
H
Matsuura
Teruhiko
T
Yoshihara
D000085:Acetates / Q000737:chemistry; D001203:Ascomycota / Q000378:metabolism; D003510:Cyclohexanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003517:Cyclopentanes / Q000737:chemistry / Q000378:metabolism; D004852:Epoxy Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development; D005875:Gibberellins / Q000096:biosynthesis / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry; D008027:Light; D054883:Oxylipins; D018547:Plant Stems / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development; D018724:Spinacia oleracea / Q000033:anatomy & histology / Q000187:drug effects* / Q000254:growth & development*
D000085:Acetates; D003510:Cyclohexanes; D003517:Cyclopentanes; D004852:Epoxy Compounds; D005875:Gibberellins; D005960:Glucosides; D054883:Oxylipins; C496818:theobroxide; C421181:tuberonic acid glucoside; C421182:tuberonic acid; C011006:jasmonic acid; C007842:gibberellic acid