Potentiation of humoral immune response and activation of NF-kappaB pathway in lymphocytes in experimentally induced hyperthyroid rats.
This study explored the effect of hyperthyroid state on humoral immune response and NF-kappaB signaling in lymphocytes. Male Wistar rats were treated with l-thyroxin for four weeks. Animals were immunized with sheep red blood cells (SRBC) after three weeks of l-thyroxin treatment. After one week of immunization, serum anti-SRBC titer was measured and NF-kappaB signaling was studied in lymphocytes by Western blot analysis of p-IKB-alpha, IKB-alpha, and p65. These results were compared with that of control rats. Antibody response and density of p-IKB-alpha and p65 were significantly higher in l-thyroxin treated rats in comparison to controls. The antibody response was found to have significant correlation with density of p-IKB-alpha and p65. Our results indicate that NF-kappaB signaling pathway in lymphocytes is activated in hyperthyroid state which might play a role in potentiation of antibody response.
Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India.
R
R
Vinayagamoorthi
Bidhan Chandra
BC
Koner
S
S
Kavitha
D N
DN
Nandakumar
P
P
Padma Priya
Kalyan
K
Goswami
D000818:Animals; D000906:Antibodies / Q000097:blood; D000917:Antibody Formation / Q000276:immunology; D015153:Blotting, Western; D004912:Erythrocytes / Q000276:immunology; D006980:Hyperthyroidism / Q000276:immunology*; D020672:I-kappa B Proteins / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D016328:NF-kappa B / Q000276:immunology*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D018709:Statistics, Nonparametric; D013974:Thyroxine / Q000276:immunology
D000906:Antibodies; D020672:I-kappa B Proteins; D016328:NF-kappa B; D013974:Thyroxine