Mineralization of 4-aminobenzenesulfonate (4-ABS) by Agrobacterium sp. strain PNS-1.
A bacterial strain, PNS-1, isolated from activated sludge, could utilize sulphanilic acid (4-ABS) as the sole organic carbon and energy source under aerobic conditions. Determination and comparison of 16S r DNA sequences showed that the strain PNS-1 is closely related to the species of Agrobacterium genus. Growth on 4-ABS was accompanied with ammonia and sulfate release. TOC results showed complete mineralization of sulphanilic acid. This strain was highly specific for 4-ABS as none of the sulphonated aromatics used in the present study including other ABS isomers were utilized. Strain PNS-1 could, however, utilize all the tested monocyclic aromatic compounds devoid of a sulfonate group. No intermediates could be detected either in the growth phase or with dense cell suspensions. Presence of chloramphenicol completely inhibited 4-ABS degradation by cells pregrown on succinate, indicating that degradation enzymes are inducible. No plasmid could be detected in the Agrobacterium sp. Strain PNS-1 suggesting that 4-ABS degradative genes may be chromosomal encoded.
Singh
Poonam
P
Department of Chemistry, Indian Institute of Technology, 208016, Kanpur, India.
Birkeland
Nils-Kåre
NK
Iyengar
Leela
L
Gurunath
Ramanathan
R
D001565:Benzoates / Q000378:metabolism; D001673:Biodegradation, Environmental / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology; D004275:DNA, Ribosomal / Q000032:analysis; D012231:Rhizobium / Q000187:drug effects / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D013425:Sulfanilic Acids / Q000378:metabolism*
D001565:Benzoates; D004275:DNA, Ribosomal; D012722:Sewage; D013425:Sulfanilic Acids; C100016:4-sulfanilic acid; D002701:Chloramphenicol