Isolation of DNA from tumor samples.
A method for producing high-molecular-weight DNA from pulverized tissue, nuclear fractions, or cultured cells. This isolation method relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent sodium dodecyl sulfate. Ethylenediaminetetraacetic acid is included in the lysis buffer to inhibit DNases.
Cancer Research UK Molecular Oncology Laboratories, Weatherall Institute of Molecular Medicine, The John Radcliffe Hospital, Oxford, UK.
Naomi
N
Robertson
Russell
R
Leek
D004273:DNA, Neoplasm / Q000302:isolation & purification* / Q000378:metabolism; D019286:Endopeptidase K / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
D004273:DNA, Neoplasm; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D019286:Endopeptidase K