Porphyromonas gingivalis lipids and diseased dental tissues.
OBJECTIVE
Porphyromonas gingivalis synthesizes several classes of dihydroceramides and at least one of these lipid classes promotes proinflammatory secretory reactions in gingival fibroblasts as well as alters fibroblast morphology in culture. The purpose of this investigation was to determine whether the dihydroceramide lipids of P. gingivalis are recovered in lipid extracts of subgingival plaque, diseased teeth, and diseased gingival tissue samples.


METHODS
Lipids were extracted from P. gingivalis, subgingival plaque, subgingival calculus, teeth laden with gross accumulations of subgingival calculus, and gingival tissue samples obtained from chronic severe periodontitis sites. Lipid samples were analyzed by gas chromatography-mass spectrometry as trimethylsilyl derivatives or by electrospray-mass spectrometry as underivatized products. High-performance liquid chromatography fractions of P. gingivalis lipids and gingival tissue lipids were also analyzed by electrospray-mass spectrometry analysis.


RESULTS
P. gingivalis phosphorylated dihydroceramides were recovered in lipid extracts of subgingival plaque, subgingival calculus, calculus contaminated teeth, and diseased gingival tissue samples. However, the distribution of phosphorylated dihydroceramides varied between these samples.


CONCLUSIONS
Subgingival plaque, subgingival calculus, diseased teeth, and gingival tissue are contaminated with phosphorylated dihydroceramides produced by P. gingivalis. The previously reported biological activity of these substances together with the recovery of these lipids at periodontal disease sites argues strongly for their classification as virulence factors in promoting chronic inflammatory periodontal disease.
Department of Oral Health and Diagnostic Sciences, University of Connecticut School of Dental Medicine, Farmington, CT, USA. nichols@nso.uchc.edu
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D002518:Ceramides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003728:Dental Calculus / Q000737:chemistry; D003773:Dental Plaque / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D005881:Gingiva / Q000737:chemistry; D005994:Glycerophosphates / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry; D010518:Periodontitis / Q000378:metabolism / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000737:chemistry*; D013109:Sphingomyelins / Q000032:analysis; D014070:Tooth / Q000737:chemistry; D014297:Trimethylsilyl Compounds / Q000032:analysis; D037521:Virulence Factors / Q000032:analysis
D002518:Ceramides; D005994:Glycerophosphates; D013109:Sphingomyelins; D014297:Trimethylsilyl Compounds; D037521:Virulence Factors; C109343:dihydroceramide; C060281:ceramide phosphoethanolamine