Long-lasting changes in small intestinal transport following the recovery from Trichinella spiralis infection.
Changes in intestinal motility and visceral sensitivity are found after resolution of acute enteric inflammation. The study investigates whether a transient nematode-induced intestinal inflammation may result in long-lasting remodelling of epithelial transport. Ferrets infected with Trichinella spiralis or sham-infected animals were euthanized on day 10, 30 or 60 postinfection (PI) and the jejunum was isolated. The net transport of electrolytes was measured electrophysiologically as transmucosal short-circuit current (I(sc)) and responses to electrical field stimulation (EFS: 1-32 Hz) or secretagogues were investigated. Myeloperoxidase (MPO) activity, a marker of mucosal inflammation, was maximal during the enteric stage of T. spiralis infection (day 10 PI) and returned to normal on days 30 and 60 PI. Mucosal inflammation caused a reduction in basal I(sc), increased electrical conductance (G) and decreased the maximal responses to EFS, carbachol or histamine. On days 30 and 60 PI the inflammation resolved and basal electrogenic transport appeared normal; however, the secretion induced by EFS, carbachol or histamine remained suppressed. Moreover, EFS-induced responses were shifted from predominantly cholinergic in controls to non-cholinergic in the infected animals. The results suggest that a transient small intestinal inflammation causes a long-term remodelling of epithelial function.
Oklahoma Center for Neuroscience, VA Medical Center, University of Oklahoma Health Science Center, Oklahoma City, OK 73104, USA.
K
K
Venkova
B
B
Greenwood-van Meerveld
D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002217:Carbachol / Q000494:pharmacology; D018679:Cholinergic Agonists / Q000494:pharmacology; D004195:Disease Models, Animal; D004558:Electric Stimulation; D004573:Electrolytes / Q000378:metabolism; D017615:Enteric Nervous System / Q000187:drug effects / Q000469:parasitology / Q000503:physiopathology; D005289:Ferrets; D006632:Histamine / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000469:parasitology / Q000503:physiopathology*; D007583:Jejunum / Q000187:drug effects / Q000469:parasitology / Q000503:physiopathology*; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D017160:Trichinella spiralis; D014235:Trichinellosis / Q000473:pathology / Q000503:physiopathology*
D018679:Cholinergic Agonists; D004573:Electrolytes; D006632:Histamine; D002217:Carbachol; D009195:Peroxidase