In vitro short-time killing activity of povidone-iodine (Isodine Gargle) in the presence of oral organic matter.
In order to estimate the clinical efficacy of a povidone-iodine oral antiseptic (PVP-I) on oral bacterial infectious diseases, we studied the effect of oral organic matter on the in vitro killing activity of PVP-I. In addition, we compared the in vitro short-time killing activity of PVP-I with those of other oral antiseptics using mouth-washing and gargling samples collected from healthy volunteers. When any of the mouth-washing and gargling samples was used, the standard (0.23-0.47%) or lower concentrations of PVP-I killed methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, including multidrug-resistant strains, within 15-60 s in the presence of oral organic matter. 0.02% benzethonium chloride (BEC) and 0.002% chlorhexidine gluconate (CHG) did not show effects against MRSA and P. aeruginosa (including multidrug-resistant strains) in mouth-washing and gargling samples even after 60 s. The above-mentioned results show that the in vitro killing activity of the standard concentration of PVP-I was hardly affected by the oral organic matter and that a mouth-washing or gargling solution containing PVP-I has a stronger bactericidal activity than BEC and CHG. Although mouth-washing and gargling samples were obtained from healthy individuals in this study, PVP-I may be used for protection against infections in patients with various diseases, if proper concentrations and usage are encouraged.
Department of Clinical Laboratory, Toranomon Hospital, Tokyo, Japan. yoneyama@toranomon.gr.jp
Akiko
A
Yoneyama
Masaki
M
Shimizu
Makiko
M
Tabata
Junko
J
Yashiro
Toshihiko
T
Takata
Muneo
M
Hikida
D000890:Anti-Infective Agents / Q000494:pharmacology*; D001558:Benzethonium / Q000494:pharmacology; D001826:Body Fluids / Q000382:microbiology; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D015169:Colony Count, Microbial; D006801:Humans; D066298:In Vitro Techniques; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology*; D009067:Mouthwashes / Q000494:pharmacology*; D011206:Povidone-Iodine / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*; D013997:Time Factors
D000890:Anti-Infective Agents; D009067:Mouthwashes; D001558:Benzethonium; D011206:Povidone-Iodine; C010882:chlorhexidine gluconate; D002710:Chlorhexidine