SECRET AGENT and SPINDLY have overlapping roles in the development of Arabidopsis thaliana L. Heyn.
O-GlcNAc transferase (OGT) catalyses transfer of GlcNAc (N-acetylglucosamine) to serine or threonine of proteins. The Arabidopsis OGTs, SECRET AGENT (SEC) and SPINDLY (SPY) have overlapping functions during gametogenesis and embryogenesis. SPY functions in a number of processes including circadian, light, and gibberellin (GA) responses. The role of SEC in plant development and GA signalling was investigated by determining the phenotypes of sec-1 and sec-2 plants and the expression pattern of SEC. Similar to SPY, SEC transcripts were ubiquitous. Although there is no evidence of transcript-level regulation by other factors, SEC mRNA levels are elevated in spy plants and SPY mRNA levels are elevated in sec plants. sec-1 and sec-2 plants exhibited few of the defects observed in spy plants and had wild-type GA responses. Compared with wild type, sec plants produced leaves at a reduced rate. Haplo-insufficiency at SEC in a spy ga1 double mutant background suppressed spy during germination and enhanced the production of ovaries with four carpels by spy. By contrast, SPY haplo-insufficiency in a sec ga1 double mutant background caused a novel phenotype, production of a proliferation of pin-like structures instead of a floral shoot. These results are consistent with SEC function overlapping with SPY for leaf production and reproductive development.
Hartweck
Lynn M
LM
Department of Plant Biology, University of Minnesota, 250 Biological Sciences Center, 1445 Gortner Ave., St Paul, 55108, USA.
Genger
Ruth K
RK
Grey
William M
WM
Olszewski
Neil E
NE
D017360:Arabidopsis / Q000033:anatomy & histology / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D005875:Gibberellins / Q000378:metabolism; D007113:Immunity, Innate / Q000502:physiology; D018519:Meristem / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D009154:Mutation; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D018515:Plant Leaves / Q000254:growth & development; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*
D029681:Arabidopsis Proteins; D005875:Gibberellins; D012097:Repressor Proteins; C101465:SPY protein, Arabidopsis; C007842:gibberellic acid; D017351:N-Acetylglucosaminyltransferases; C510668:SECRET AGENT protein, Arabidopsis