[Cellular components and placental alkaline phosphatase in Trypanosoma cruzi infection ].
Trypanosoma cruzi induces changes in the protein pattern of human placenta syncytiotrophoblast. Placental alkaline phosphatase (PLAP) is a glycoenzyme anchored to the membrane by a glycosyl-phosphatidylinositol molecule. PLAP activity and its presence was altered by the parasite in cultures of human placental villi and HEp2 cells with T.cruzi. The cells treated before the cultures with agents which affect PILAP or glycosyl-phosphatidylinositol (antibodies, PL-C, genistein, lithium) presented less parasitic invasion than the control ones. It was also observed a modification in the pattern of actine filaments of the host cells infected. We concluded that PLAP would participate in the process of T. cruzi invasion into placental syncitiotrophoblast cells, by a mechanism that involves hydrolysis of the glycosyl-phosphatidylinositol molecules, the activation of tyrosine kinase proteins, the increase of cytosolic calcium and the rearrangement of actine filaments of the host cells.
Cátedra de Biología Celular, Histología y Embriología, Instituto de Biología Celular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Cordoba, Argentina. msartori@cmefcm.uncor.edu
Maria José
MJ
Sartori
Luciana
L
Mezzano
Susana
S
Lin
Gastón
G
Repossi
Sofía P
SP
Fabro
D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques; D014355:Chagas Disease / Q000201:enzymology* / Q000276:immunology / Q000469:parasitology; D002824:Chorionic Villi / Q000201:enzymology / Q000469:parasitology; D005260:Female; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D010920:Placenta / Q000201:enzymology* / Q000469:parasitology; D011247:Pregnancy; D014327:Trophoblasts / Q000201:enzymology / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000502:physiology*
D015415:Biomarkers; D017261:Glycosylphosphatidylinositols; D000469:Alkaline Phosphatase