Metabolic effects of dinoseb, diazinon and esfenvalerate in eyed eggs and alevins of Chinook salmon (Oncorhynchus tshawytscha) determined by 1H NMR metabolomics.
Pesticide pulses in the Sacramento River, California, originate from storm-water discharges and non-point source aquatic pollution that can last from a few days to weeks. The Sacramento River and its tributaries have historically supported the majority of California's Chinook salmon (Oncorhynchus tshawytscha) spawning grounds. Three pesticides currently used in the Sacramento Valley-- dinoseb, diazinon, and esfenvalerate-- were chosen to model the exposure of salmon embryos to storm-water discharges. Static-renewal (96 h) exposures to eyed eggs and alevins resulted in both toxicity and significant changes in metabolism assessed in whole-embryo extracts by (1)H nuclear magnetic resonance (NMR) spectroscopy based metabolomics and HPLC with UV detection (HPLC-UV). The 96-h LC(50) values of eyed eggs and alevins exposed to dinoseb were 335 and 70.6 ppb, respectively, and the corresponding values for diazinon were 545 and 29.5 ppm for eyed eggs and alevins, respectively. The 96-h LC(50) of eyed eggs exposed to esfenvalerate could not be determined due to lack of mortality at the highest exposure concentration, but in alevins was 16.7 ppb. All esfenvalerate exposed alevins developed some degree of lordosis or myoskeletal abnormality and did not respond to stimulus or exhibit normal swimming behavior. ATP concentrations measured by HPLC-UV decreased significantly in eyed eggs due to 250 ppb dinoseb and 10 and 100 ppb esfenvalerate (p < 0.05). Phosphocreatine, as measured by HPLC-UV, decreased significantly in eyed eggs due to 250 ppb dinoseb, 10 and 100 ppb esfenvalerate, and 100 ppm diazinon (p < 0.05). Principal components analyses of (1)H NMR metabolite fingerprints of eyed egg and alevin extracts revealed both dose-dependent and mechanism of action-specific metabolic effects induced by the pesticides. Furthermore, NMR based metabolomics proved to be more sensitive than HPLC-UV in identifying significant changes in sublethal metabolism of pesticide exposed alevins. In conclusion, we have demonstrated several benefits of a metabolomics approach for chemical risk assessment, when used in conjunction with a fish embryo assay, and have identified significant metabolic perturbations to the early life stages of Chinook salmon by currently used pesticides.
Viant
Mark R
MR
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. M.Viant@bham.ac.uk
Pincetich
Christopher A
CA
Tjeerdema
Ronald S
RS
D019297:2,4-Dinitrophenol / Q000031:analogs & derivatives* / Q000633:toxicity; D000244:Adenosine Diphosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000704:Analysis of Variance; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D003976:Diazinon / Q000633:toxicity*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D007928:Lethal Dose 50; D008141:Lordosis / Q000139:chemically induced / Q000196:embryology / Q000662:veterinary; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009570:Nitriles / Q000633:toxicity*; D010575:Pesticides / Q000633:toxicity*; D010725:Phosphocreatine / Q000032:analysis; D025341:Principal Component Analysis; D011722:Pyrethrins / Q000633:toxicity*; D018570:Risk Assessment / Q000379:methods; D012474:Salmon / Q000196:embryology* / Q000378:metabolism / Q000502:physiology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D009570:Nitriles; D010575:Pesticides; D011722:Pyrethrins; D014874:Water Pollutants, Chemical; D010725:Phosphocreatine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D019297:2,4-Dinitrophenol; C018527:dinoseb; D003976:Diazinon; C017690:fenvalerate