Recovery of Salmonella from commercial shell eggs by shell rinse and shell crush methodologies.
Salmonella is the most important human pathogen associated with shell eggs. Salmonella Enteritidis is the serotype most often implicated in outbreaks, although other serotypes have been recovered from eggs and from the commercial shell egg washing environment. Many sample methods are used to recover microorganisms from eggshells and membranes. A shell rinse and modified shell-and-membrane crush method for recovery of Salmonella were compared. Eggs were collected from 3 commercial shell-washing facilities (X, Y, and Z) during 3 visits. Twelve eggs were collected from each of 10 to 12 locations along the egg processing chain. After being transported back to the laboratory, each egg was sampled first by a shell rinse method and then by a shell crush method. For each technique (rinse or crush), 2 pools of 5 eggs per location sampled were selectively enriched for the recovery of Salmonella. Presumptive samples positive for Salmonella were confirmed serologically. Overall, there were 10.1% (40/396) Salmonella-positive pooled samples. Salmonella were recovered by the shell rinse and shell crush techniques (4.8 vs. 5.3%, respectively). Plant X yielded 21.5% Salmonella positives, whereas less than 5% of samples from plants Y and Z were found to be contaminated with the organism (4.2 and 4.5%, respectively). Salmonella was recovered more often from unwashed eggs (15.8%) than from washed eggs (8.3%). For some eggs, Salmonella was only recovered by one of the methods. Use of both approaches in the same experiment increased sampling sensitivity, although in most cases, crushing provided more sensitive Salmonella recovery.
United States Department of Agriculture, Agricultural Research Service, Richard B. Russell Agricultural Research Center, 950 College Station Road, Athens, Georgia 30605, USA. mmusgrov@saa.ars.usda.gov
M T
MT
Musgrove
D R
DR
Jones
J K
JK
Northcutt
M A
MA
Harrison
N A
NA
Cox
K D
KD
Ingram
A J
AJ
Hinton
D000818:Animals; D002645:Chickens / Q000382:microbiology*; D004203:Disinfection / Q000379:methods; D004528:Egg Shell / Q000382:microbiology*; D004531:Eggs / Q000382:microbiology; D005506:Food Contamination; D005511:Food Handling / Q000379:methods; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity