Microdevices for separation, accumulation, and analysis of biological micro- and nanoparticles.
Microfabrication and performance of a novel microsystem for separation, accumulation and analysis of biological micro- and nanoparticles is reported. Versatile chip functions based on dielectrophoresis and microfluidics were integrated to isolate particles from complex sample solutions such as serum. A bead-based assay for virus detection is proposed. Separation of micro- and sub-mum beads employing dielectrophoretic deflector and bandpass structures is demonstrated. Individual antibody coated beads with hepatitis A virus bound to their surface were trapped by negative dielectrophoresis in a field cage and analysed by fluorescence microscopy.
Kentsch
J
J
Naturwissenschaftliches und Medizinisches Institut, Reutlingen, GermanyEvotec Technologies GmbH, Hamburg, GermanyHumboldt-Universitat zu Berlin, Institut für Biologie, Berlin, GermanyFraunhofer-Institut für Biomedizinische Technik, St. Ingbert, GermanyMediagnost GmbH, Reutlingen, Germany.
Dürr
M
M
Schnelle
T
T
Gradl
G
G
Müller
T
T
Jager
M
M
Normann
A
A
Stelzle
M
M