BH3 domain mutation of proapoptotic genes Bad, Bmf and Bcl-G is rare in transitional cell carcinomas of the urinary bladder.
OBJECTIVE
Mounting evidence indicates that deregulation of apoptosis contributes to the development of human cancers. Bcl-2 family proteins regulate the intrinsic apoptosis pathway. The aim of this study was to explore the possibility that mutation of BH3 domain of proapoptotic Bcl-2 genes Bad, Bmf and Bcl-G might be involved in the development of urinary bladder cancer.


METHODS
We analysed the BH3 domains of Bad, Bmf and Bcl-G genes for the detection of somatic mutations in 43 transitional cell carcinomas (TCCs) of the urinary bladder by a single strand conformation polymorphism assay in this study.


RESULTS
There was no somatic mutation of BH3 domains of Bad, Bmf and Bcl-G genes in the TCC samples.


CONCLUSIONS
The data presented here indicate that BH3 domain mutation of these genes is rare in TCCs and may not contribute to the pathogenesis of TCCs.
Soung
Young Hwa
YH
Department of Pathology, College of Medicine, The Catholic University of Korea, Socho-gu, Seoul, Korea.
Lee
Jong Woo
JW
Park
Won Sang
WS
Nam
Suk Woo
SW
Lee
Jung Young
JY
Yoo
Nam Jin
NJ
Lee
Sug Hyung
SH
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000502:physiology; D017209:Apoptosis / Q000235:genetics*; D051023:BH3 Interacting Domain Death Agonist Protein / Q000235:genetics* / Q000502:physiology; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000503:physiopathology; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000502:physiology; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000503:physiopathology; D051025:bcl-Associated Death Protein / Q000235:genetics* / Q000502:physiology
D048868:Adaptor Proteins, Signal Transducing; C494819:BAD protein, human; C428448:BCL2L14 protein, human; D051023:BH3 Interacting Domain Death Agonist Protein; C494814:BID protein, human; C486655:BMF protein, human; D004273:DNA, Neoplasm; D019253:Proto-Oncogene Proteins c-bcl-2; D051025:bcl-Associated Death Protein