[Preliminary study on the alpha-glucosidase inhibitor from Glycyrrhiza uralensis Fisch].
OBJECTIVE
To extract and isolate alpha-glucosidase inhibitor from Glycyrrhiza uralensis Fisch.


METHODS
The aqueous extract of Glycyrrhiza uralensis Fisch. was extracted by petroleum, ethyl acetate and n-butanol. In addition, the inhibitory activity against alpha-glucosidase of every fraction was determined and the inhibitory kinetics of ethyl acetate fraction was investigated.


RESULTS
The inhibitory activities of four fractions were 68.93%, 83.2%, 32.17% and 10.79% respectively. Compared with the aqueous extract (69.77%), the inhibitory activity of ethyl acetate fraction was higher. Ethyl acetate fraction was shown to be a fast-binding, dose-dependent and competitive model with a Ki value of 34 microg/ml.


CONCLUSIONS
It is hoped to obtain good alpha-glucosidase inhibitors by separating ethyl acetate fraction of aqueous extract of Glycyrrhiza uralensis Fisch further.
Xu
Yourui
Y
School of Pharmacy, Lanzhou University, Lanzhou 730000.
Ni
Jingman
J
Meng
Qinggang
Q
Gao
Yan
Y
Fan
Bin
B
D000085:Acetates / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification* / Q000494:pharmacology; D065089:Glycoside Hydrolase Inhibitors; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014867:Water; D000520:alpha-Glucosidases / Q000302:isolation & purification / Q000494:pharmacology
D000085:Acetates; D004365:Drugs, Chinese Herbal; D065089:Glycoside Hydrolase Inhibitors; D014867:Water; C007650:ethyl acetate; D000520:alpha-Glucosidases