p8 Upregulation sensitizes astrocytes to oxidative stress.
Here we studied the mechanism of cell sensitization to oxidative stress by analyzing the gene expression profile of serum-deprived astrocytes. Exposure to serum-free medium (i) sensitized astrocytes to oxidative stress, (ii) reduced the expression of several genes involved in protection against oxidative stress, including heme oxygenase 1, and (iii) changed the expression of several genes involved in the control of cell survival, including the stress-regulated protein p8. Our results support that serum deprivation sensitizes astrocytes to oxidative stress via a p38 mitogen-activated protein kinase-dependent p8 upregulation that leads in turn to decreased heme oxygenase 1 expression.
Department of Biochemistry and Molecular Biology I, School of Biology, Complutense University, c/José Antonio Novais s/n, 28040 Madrid, Spain.
Arkaitz
A
Carracedo
Ainara
A
Egia
Manuel
M
Guzmán
Guillermo
G
Velasco
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D051547:Heme Oxygenase-1 / Q000235:genetics; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D051792:Basic Helix-Loop-Helix Proteins; D051547:Heme Oxygenase-1; D009363:Neoplasm Proteins; D048051:p38 Mitogen-Activated Protein Kinases; C109741:Nupr1 protein, rat