A visual approach to proteomics.
Cryo-electron tomography is an emerging imaging technique that has unique potential for molecular cell biology. At the present resolution of 4-5 nm, large supramolecular structures can be studied in unperturbed cellular environments and, in the future, it will become possible to map molecular landscapes inside cells in a more comprehensive manner. 'Visual proteomics' aims to complement and extend mass-spectrometry-based inventories, and to provide a quantitative description of the macromolecular interactions that underlie cellular functions.
Nickell
Stephan
S
Max Planck Institute for Biochemistry, Department of Structural Biology, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Kofler
Christine
C
Leis
Andrew P
AP
Baumeister
Wolfgang
W
D020285:Cryoelectron Microscopy / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure*; D020543:Proteome; D000461:Rhodophyta / Q000648:ultrastructure
D011506:Proteins; D020543:Proteome