Routine markerless gene replacement in Bacillus anthracis.
An improved genetic tool suitable for routine markerless allelic exchange in Bacillus anthracis has been constructed. Its utility was demonstrated by the introduction of insertions, deletions, and missense mutations on the chromosome and plasmid pXO1 of the Sterne strain of B. anthracis.
Division of Bacterial Products, CBER/FDA, 8800 Rockville Pike, Bethesda, MD 20892, USA.
Brian K
BK
Janes
Scott
S
Stibitz
D001408:Bacillus anthracis / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005819:Genetic Markers; D016680:Genome, Bacterial; D010957:Plasmids / Q000235:genetics*
D005819:Genetic Markers