Application of immunoproteomics to rapid cytokine detection.
Cytokines are pivotal to a balanced innate or cell-mediated immune response, and can be indicative of disease progression and/or resolution. Methods to measure key cytokines rapidly with accuracy, precision, and sensitivity are consequently important. The current assay technologies, which are based on RT-PCR, immunoassays, or bioassays, are limited in their use in the clinic, in particular because of the long time (1-3 h) required to carry out the assays. An alternative, semi-quantitative approach described here, uses an immunological capture step and a mass spectral readout. The goal of the assay is speed rather than sensitivity or precision.
Department of Biology, Juniata College, Huntingdon, PA 16652, USA. boyle@juniata.edu
Michael D P
MD
Boyle
Jennifer L
JL
Hess
Anthony A
AA
Nuara
R Mark
RM
Buller
D000818:Animals; D001681:Biological Assay / Q000379:methods; D018925:Chemokines / Q000032:analysis* / Q000378:metabolism; D016207:Cytokines / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007249:Inflammation; D007372:Interferons / Q000378:metabolism; D013058:Mass Spectrometry; D040081:Protein Array Analysis / Q000379:methods; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D018925:Chemokines; D016207:Cytokines; D007372:Interferons