An antiviral protein having deoxyribonuclease and ribonuclease activity from leaves of the post-flowering stage of Celosia cristata.
An antiviral protein named CCP-27 was purified from the leaves of Celosia cristata at the post-flowering stage by anion-exchange, cation-exchange, and gel-filtration chromatography. It exhibited resistance against sunnhemp rosette virus in its test host Cyamopsis tetragonoloba. It also exhibited deoxyribonuclease activity against supercoiled pBlueScript SK+ plasmid DNA. It was found to nick supercoiled DNA into nicked circular form at lower protein concentration followed by nicked to linear form conversion at higher protein concentration. CCP-27 also possesses strong ribonuclease activity against Torula yeast rRNA.
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi-110012, India. mb_bioc@yahoo.com
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Begam
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Narwal
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Roy
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Kumar
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Lodha
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Kapoor
D000996:Antitussive Agents / Q000737:chemistry* / Q000302:isolation & purification; D034762:Celosia / Q000737:chemistry*; D003454:Cryptococcus / Q000737:chemistry; D004278:DNA, Superhelical / Q000737:chemistry; D003851:Deoxyribonucleases / Q000737:chemistry* / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010957:Plasmids / Q000737:chemistry; D012335:RNA, Ribosomal / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry* / Q000302:isolation & purification
D000996:Antitussive Agents; D004278:DNA, Superhelical; D010940:Plant Proteins; D012335:RNA, Ribosomal; D003851:Deoxyribonucleases; D012260:Ribonucleases