Analysis and properties of the yeast YIP1 family of Ypt-interacting proteins.
The YIP1 family of proteins is an intriguing collection of small membrane proteins with critical roles in membrane traffic. Although their mode of action is unknown, they are receiving attention as participants in vesicle biogenesis, and as factors that may mediate the association of Rab proteins with membranes. Yeast YIP1 is an essential gene and can be fully complemented by its human counterpart-suggesting that the essential function of Yip1p is evolutionarily conserved. This chapter presents methods for the cell biological and genetic analysis of Yip1p and other YIP1 family members in the yeast Saccharomyces cerevisiae.
Catherine Z
CZ
Chen
Ruth N
RN
Collins
D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D033921:Vesicular Transport Proteins
D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; C414462:Yip1 protein, S cerevisiae