Assays for cytokines using aptamers.
Aptamers are short nucleic acid sequences that are used as ligands to bind their targets with high affinity. They are generated via the combinatorial chemistry procedure systematic evolution of ligands by exponential enrichment (SELEX). Aptamers have shown much promise towards detection of a variety of protein targets, including cytokines. Specifically, for the determination of cytokines and growth factors, several assays making use of aptamers have been developed, including aptamer-based enzyme-linked immunosorbent assays, antibody-linked oligonucleotide assay, fluorescence (anisotropy and resonance energy transfer) assays, and proximity ligation assays. In this article, the concept of aptamer selection using SELEX and the assay formats using aptamers for the detection of cytokines are discussed.
Guthrie
Jeffrey W
JW
Environmental Health Sciences, Department of Public Health Sciences, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alta., Canada T6G 2G3.
Hamula
Camille L A
CL
Zhang
Hongquan
H
Le
X Chris
XC
D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D001671:Biochemistry / Q000379:methods*; D001681:Biological Assay / Q000379:methods; D020650:Combinatorial Chemistry Techniques; D016207:Cytokines / Q000032:analysis* / Q000378:metabolism; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005454:Fluorescence Polarization; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013997:Time Factors
D052157:Aptamers, Nucleotide; D016207:Cytokines; D008024:Ligands; D009841:Oligonucleotides; D011506:Proteins; D004247:DNA