Isolation of DNA from tumor samples.
A method for producing high-molecular-weight DNA from pulverized tissue, nuclear fractions, or cultured cells. This isolation method relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent sodium dodecyl sulfate. Ethylenediaminetetraacetic acid is included in the lysis buffer to inhibit DNases.
Robertson
Naomi
N
Cancer Research UK Molecular Oncology Laboratories, Weatherall Institute of Molecular Medicine, The John Radcliffe Hospital, Oxford, UK.
Leek
Russell
R
D004273:DNA, Neoplasm / Q000302:isolation & purification* / Q000378:metabolism; D019286:Endopeptidase K / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
D004273:DNA, Neoplasm; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D019286:Endopeptidase K