Biosynthesis of medium-chain-length poly(hydroxyalkanoates) from soy molasses.
Pseudomonas corrugata was selected from a screening process for the bioconversion of inexpensive soy molasses into medium-chain-length poly(hydroxyalkanoates) (mcl-PHA). We obtained yields of 1.5 g cell dry weight (CDW)/l culture with growth medium supplemented with 2% (w/v) soy molasses, and of an average of 3.4 g CDW/l with 5% (w/v) soy molasses. Crude PHAs were obtained at 5-17% of CDW. The most prominent repeat-unit monomers in the PHAs were 3-hydroxydodecanoate, 3-hydroxyoctanoate, 3-hydroxydodecanoate, and 3-hydroxytetradecenoate. This work represents the first description of fermentative mcl-PHA production from the soy molasses.
Fats, Oils and Animal Coproducts Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, Pennsylvania, 19038, USA. dsolaiman@errc.ars.usda.gov
Daniel K Y
DK
Solaiman
Richard D
RD
Ashby
Arland T
AT
Hotchkiss
Thomas A
TA
Foglia
D002210:Caprylates / Q000378:metabolism*; D007850:Lauric Acids / Q000378:metabolism*; D008965:Molasses / Q000382:microbiology*; D009227:Myristic Acids / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry*
D002210:Caprylates; D007850:Lauric Acids; D009227:Myristic Acids; C022145:3-hydroxydodecanoic acid; C009893:beta-hydroxymyristic acid; C101940:3-hydroxyoctanoic acid