Metallothionein gene from Tetrahymena thermophila with a copper-inducible-repressible promoter.
We describe a novel metallothionein gene from Tetrahymena thermophila that has a strong copper-inducible promoter. This promoter can be turned on and off rapidly, making it a useful system for induction of ectopic gene expression in Tetrahymena and enhancing its applications in cell and molecular biology, as well as biotechnology.
Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35100 Padova, Italy. francesco.boldrin@unipd.it
Francesco
F
Boldrin
Gianfranco
G
Santovito
Jacek
J
Gaertig
Dorota
D
Wloga
Donna
D
Cassidy-Hanley
Theodore G
TG
Clark
Ester
E
Piccinni
D000818:Animals; D003300:Copper / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008668:Metallothionein / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins; D016808:Tetrahymena thermophila / Q000166:cytology / Q000187:drug effects / Q000235:genetics*
D012333:RNA, Messenger; D011994:Recombinant Proteins; D003300:Copper; D008668:Metallothionein