[Proteomic analysis of proteins related to retinoid acid resistance].
OBJECTIVE
To evaluate the applicability of proteomic methods for shedding light on the mechanisms of All-trans retinoic acid resistance.


METHODS
The expression of cellular proteins in the retinoid acid sensitive cell line NB4 and retinoid acid resistant cell line MR2 was analyzed using the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Differentially expressed proteins were analyzed by mass spectrometry for peptide mass finger and identified by SWISS-PROT protein database.


RESULTS
Approximately 600 spots appeared in an sodium dod(SDS-GEL). The match of the expression of cellular proteins between NB4 and MR2 was better. Three significantly differentially expressed proteins were screened and identified to DJ-1 and HSP70, as well as KIAA1289 of which the function is not yet known.


CONCLUSIONS
The utilization of 2D-PAGE, coupled with mass spectrometry is effective in screening the drug resistance-associated proteins and could provide molecular targets for the elucidation of resistance mechanisms.
Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
Hui
H
Qin
Ting
T
Liu
Jin-liang
JL
Yang
Han-shou
HS
Yang
Wen-tong
WT
Meng
Bing
B
Kan
Yu-quan
YQ
Wei
Xia
X
Zhao
Bin
B
Liu
Xin
X
Song
D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis*; D013058:Mass Spectrometry; D015513:Oncogene Proteins / Q000032:analysis*; D010449:Peptide Mapping; D040081:Protein Array Analysis; D000071617:Protein Deglycase DJ-1; D040901:Proteomics / Q000379:methods*; D014212:Tretinoin / Q000494:pharmacology*
D000970:Antineoplastic Agents; D018840:HSP70 Heat-Shock Proteins; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D014212:Tretinoin; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1