Analyses of Stemona alkaloids in Stemona tuberosa by liquid chromatography/tandem mass spectrometry.
Alkaloid profiles in Stemona tuberosa were found to be highly variable. Six Stemona alkaloids isolated from the plant were subjected to on-line high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) and tandem mass spectrometry (MS/MS) analyses. Their fragmentation patterns and products were useful for their characterization. The LC/MS fingerprints of these alkaloids, though variable among samples, could provide an overall characterization of the authenticity and quality of this species and help to differentiate it from S. japonica and S. sessilifolia, as all three species are recognized as genuine sources of the herb Radix Stemonae in the Pharmacopoeia of the People's Republic of China.
Zhou
Yan
Y
Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, PR China.
Jiang
Ren-Wang
RW
Hon
Po-Ming
PM
Xu
Yan-Tong
YT
Chan
Yiu-Man
YM
Chan
Tak-Wah Dominic
TW
Xu
Hong-Xi
HX
Ding
Li-Sheng
LS
But
Paul Pui-Hay
PP
Shaw
Pang-Chui
PC
D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015394:Molecular Structure; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D041901:Stemonaceae / Q000737:chemistry*
D000470:Alkaloids