Partial sequence of connectin-like 1200K-protein in obliquely striated muscle of a polychaete (Annelida): evidence for structural diversity from vertebrate and invertebrate connectins.
Vertebrate striated muscle contains the giant elastic protein connectin that maintains the position of the A-band at the center of the sarcomere during repeated muscular contraction and relaxation. Connectin-like molecules may perform conserved functions in vertebrate and invertebrate striated and oblique muscles, although less is known about the structure of invertebrate connectins at present. The protein that maintains such a structure is present not only in vertebrate striated muscle, but also in invertebrate striated and oblique muscle. In the present study, we analyzed the partial primary structure of a 1200K-protein, which is a connectin-like protein that is expressed in Neanthes sp. body wall muscle that is in turn composed of oblique muscle. Antibody screening of a cDNA library of Neanthes sp. body wall muscle identified two different clones. Both clones coded for a sequence predominantly comprised of the four amino acids proline (P), glutamate (E), valine (V) and lysine (K). One clone included a PEVK-like repeat sequence flanked by an Ig domain, while the other clone comprised a distinct 14 amino acid repeat rich in PEVK residues, flanked by a non-repetitive unique sequence. The PEVK region is found in vertebrate connectin and is thought to generate elasticity and be responsible for passive tension of the muscle. The antibodies produced against a portion of each clone both reacted with bands corresponding to 1200 kDa present in Neanthes sp. body wall muscle. Therefore, our results demonstrate that this 1200K-protein is a connectin-like elastic protein and includes specific PEVK-like fragment. We suggest that this 1200K-protein plays a major role in maintaining the structure of oblique muscle in invertebrates.
Izawa
Noboru
N
Department of Biology, Faculty of Science, Chiba University, 263-8522, Chiba, Japan.
Fukuzawa
Atsushi
A
Kanzawa
Nobuyuki
N
Kawamura
Yuuki
Y
Maruyama
Koscak
K
Kimura
Sumiko
S
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western; D003001:Cloning, Molecular; D064211:Connectin; D016384:Consensus Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D007448:Invertebrates / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011077:Polychaeta / Q000235:genetics* / Q000378:metabolism; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D014714:Vertebrates / Q000235:genetics
D000906:Antibodies; D064211:Connectin; D018076:DNA, Complementary; D009124:Muscle Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D011494:Protein Kinases