Redox cycling of adrenaline and adrenochrome catalysed by mitochondrial Complex I.
Complex I in bovine heart submitochondrial particles catalyses the NADH-supported generation of superoxide anion; adrenaline is oxidised by superoxide to adrenochrome that, on its hand, is reduced by Complex I, thus establishing a redox cycle that amplifies the superoxide production. The routes in Complex I for superoxide formation and for adrenochrome reduction appear to be different, since they have a different sensitivity to Complex I inhibitors. The results are discussed in terms of current assays for superoxide detection and of pathologies linked to catecholamine oxidation.
Dipartimento di Biochimica "G. Moruzzi," Università di Bologna, Via Irnerio 48, 40126 Bologna, Italy. marialuisa.genova@unibo.it
Maria Luisa
ML
Genova
Nagwa M
NM
Abd-Elsalam
El Sayed M E
el SM
Mahdy
Andrea
A
Bernacchia
Marco
M
Lucarini
Gian Franco
GF
Pedulli
Giorgio
G
Lenaz
D000323:Adrenochrome / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D002417:Cattle; D002478:Cells, Cultured; D042967:Electron Transport Complex I / Q000378:metabolism*; D004837:Epinephrine / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides; D000323:Adrenochrome; D042967:Electron Transport Complex I; D004837:Epinephrine