Expression of the regulatory protein LndI for landomycin E production in Streptomyces globisporus 1912 is controlled by the availability of tRNA for the rare UUA codon.
The gene lndI encodes the activator of landomycin biosynthesis. The utilization of LndI-EGFP fusions led us to investigate the temporal pattern of this gene expression and demonstrated the delay between lndI transcription and translation. The TTA codon in lndI is thought to be the reason for this delay. The replacement of TTA with CTC cancelled the pause between lndI transcription and the translation. The wild-type of the lndI gene is not expressed in the Streptomyces coelicolor bldA- mutant strain, indicating the importance of the bldA tRNA in its mRNA translation.
Rebets
Yuriy V
YV
Department of Genetics and Biotechnology of Ivan Franko National University of L'viv, L'viv, Ukraine.
Ostash
Bohdan O
BO
Fukuhara
Masahiro
M
Nakamura
Tatsunosuke
T
Fedorenko
Victor O
VO
D000617:Aminoglycosides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D015153:Blotting, Western / Q000379:methods; D003062:Codon / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D023061:Gene Order; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D016297:Mutagenesis, Site-Directed / Q000379:methods; D009154:Mutation / Q000502:physiology; D012343:RNA, Transfer / Q000502:physiology*; D013302:Streptomyces / Q000235:genetics / Q000502:physiology*; D013997:Time Factors; D014158:Transcription, Genetic / Q000235:genetics
D000617:Aminoglycosides; D003062:Codon; D005456:Fluorescent Dyes; C485184:enhanced green fluorescent protein; C113593:landomycin E; D049452:Green Fluorescent Proteins; D012343:RNA, Transfer