[A preliminary analysis of the function of the OsRab5a gene in rice (Oryza sativa L.)].
OsRab5a encodes a Rab5 protein in rice, which belongs to the Rab family of small GTP-binding protein superfamily. Transgenic plants with POsrab5a::beta-glucuronidase (GUS) gene indicate that OsRab5a is expressed in callus, root, stem, leaf, root base and spikelet (Figs.1, 2H-1). Using the transgenic Arabidopsis with fused gene of GFP::OsRab5a and GFP::OsRab5aCA and by using FM4-64 (the lipophilic styryl dye) staining, we have demonstrated that OsRab5a is involved in vesicular transport and GFP-OsRab5a is localized on the plasma membrane and early endosomes (Fig.2A-C), while GFP-OsRab5aCA is localized in the cell membrane (Fig.2D-F). The reduction of expression of OsRab5a in callus RNA interference, however, led to the lethality of callus during differentiation (Fig.3D-F). The expression of OsRab5a was upregulated slightly by exogenous cytokinin (Fig.4), and was higher in the differentiation medium than that in selection medium (Fig.3G-I). These results suggest that OsRab5a plays an important role in callus differentiation probably through mediating hormone signal transduction.
Jia
Qiao-Jun
QJ
College of Life Sciences, Zhejiang University, Hangzhou 310029, China.
Qi
Xiao-Peng
XP
Wu
Ping
P
D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D003583:Cytokinins / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D017343:Genes, Plant / Q000502:physiology; D006728:Hormones / Q000494:pharmacology; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000235:genetics*; D014945:Wound Healing / Q000502:physiology; D020696:rab5 GTP-Binding Proteins / Q000378:metabolism / Q000502:physiology*
D003583:Cytokinins; D006728:Hormones; D018749:RNA, Plant; D020696:rab5 GTP-Binding Proteins