Lower macrophage recruitment and atherosclerosis resistance in FVB mice.
The aim of this study was to compare some aspects of cholesterol accretion and cholesterol efflux in cellular components of the aortic wall derived from mice resistant or susceptible to atherosclerosis, FVB or C57BL, respectively. Cholesterol efflux, from cholesterol loaded smooth muscle cells or elicited macrophages, to apo A-I or HDL was similar in the two strains under basal conditions, and after cAMP or LXR upregulation. Recruitment of peritoneal macrophages, 3 days after thioglycollate injection, was 65% lower in FVB than in C57BL mice, commensurate with a 40% reduction in MCP-1 in peritoneal lavage. In additional three atherosclerosis resistant strains, NZB, A/J and 129(SvJ), macrophage recruitment was reduced to a similar extent despite high MCP-1 levels. Since impaired macrophage recruitment in CCR2(-/-) or MCP-1(-/-) C57BL mice was reported to reduce atherosclerosis, it seems plausible that in some mouse strains reduction in macrophage mobilization could contribute to atherosclerosis resistance.
Department of Experimental Medicine and Cancer Research, Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel.
Olga
O
Stein
Yedida
Y
Dabach
Mazal
M
Ben-Naim
Gideon
G
Halperin
Yechezkiel
Y
Stein
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism / Q000473:pathology; D050197:Atherosclerosis / Q000378:metabolism* / Q000473:pathology; D018932:Chemokine CCL2 / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D004195:Disease Models, Animal; D018450:Disease Progression; D017737:Macrophages, Peritoneal / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008814:Mice, Inbred NZB; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000473:pathology; D010743:Phospholipids / Q000378:metabolism
C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D010743:Phospholipids; D002784:Cholesterol