[Relief effect of beta-galactosidase genetically engineered lactococcus lactis on the cell toxicity caused by lactose].
OBJECTIVE
To assess the relief effect of beta-galactosidase genetically engineered Lactococcus lactis on the cell toxicity caused by lactose in vitro.


METHODS
An in vitro toxic Caco-2 cell model caused by lactose was established to evaluate the relief effect of beta-galactosidase genetically engineered Lactococcus lactis. Cell morphological parameters and proliferation activity parameter were used.


RESULTS
The in vitro toxic Caco-2 cell model caused by lactose was successfully established; the genetically engineered Lactococcus lactis constructed in the authors' laboratory could enable the Caco-2 cell to have normal appearance with the presence of lactose and could improve the proliferation activity with the presence of high concentration of lactose (P < 0.01).


CONCLUSIONS
The beta-galactosidase genetically engineered Lactococcus lactis has significant relief effect on the cell toxicity caused by lactose in vitro, which lays a foundation for food-grade alternation of this bacterium.
Liu
Xiang
X
Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Lü
Xiao-ying
XY
Yu
Qian
Q
Zhang
Chao-wu
CW
D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D005818:Genetic Engineering; D006801:Humans; D013294:Lactococcus lactis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D007785:Lactose / Q000378:metabolism / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
D001616:beta-Galactosidase; D007785:Lactose