Analyses of Stemona alkaloids in Stemona tuberosa by liquid chromatography/tandem mass spectrometry.
Alkaloid profiles in Stemona tuberosa were found to be highly variable. Six Stemona alkaloids isolated from the plant were subjected to on-line high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) and tandem mass spectrometry (MS/MS) analyses. Their fragmentation patterns and products were useful for their characterization. The LC/MS fingerprints of these alkaloids, though variable among samples, could provide an overall characterization of the authenticity and quality of this species and help to differentiate it from S. japonica and S. sessilifolia, as all three species are recognized as genuine sources of the herb Radix Stemonae in the Pharmacopoeia of the People's Republic of China.
Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, PR China.
Yan
Y
Zhou
Ren-Wang
RW
Jiang
Po-Ming
PM
Hon
Yan-Tong
YT
Xu
Yiu-Man
YM
Chan
Tak-Wah Dominic
TW
Chan
Hong-Xi
HX
Xu
Li-Sheng
LS
Ding
Paul Pui-Hay
PP
But
Pang-Chui
PC
Shaw
D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015394:Molecular Structure; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D041901:Stemonaceae / Q000737:chemistry*
D000470:Alkaloids