[The analysis of T cell receptor Vbeta gene usage of cytolytic T-lymphocytes in coxsackievirus B3-induced myocarditis].
OBJECTIVE
To analyze the T cell receptor (TCR) Vbeta gene usage of the cytolytic T-lymphocytes which result in immune-mediated myocyte injury in Coxsackievirus B3-induced myocarditis.


METHODS
An experimental murine model with a myocarditic variant of Coxsackievirus B, type 3 has been developed with newborn Balb/c male mice. Mice were killed on day 7 after virus inoculated, which was the time when T cells show significant differentiation. With immunoadsorption, ACTLs, VCTLs, MCTLs are respectively isolated from mesenteric lymph nodes of Balb/c mice suffered from viral myocarditis. T-lymphocytes prepared from mesenteric lymph nodes of uninfected Balb/c mice served as control group. RT-PCR was performed with standard method. The Vbeta gene usage was analyzed by running the electophoresis of PCR products on agarose gel.


RESULTS
T cells of control group expressed all Vbeta genes of 20 families. But the TCR VP gene usage of CTLs in experimental groups was markedly restricted. ACTL expressed predominantly Vbeta6, Vbeta8.1, Vbeta8.2, Vbeta8.3, MCTL expressed predominantly Vbeta5.1, Vbeta8.1, Vbeta8.2, Vbeta8.3 and VCTL expressed predominantly Vbeta7, Vbeta8.1, Vbeta8.2, Vbeta8.3.


CONCLUSIONS
The TCR Vbeta gene usage of CTLs which result in immune-mediated myocyte injury in Coxsackievirus B3-induced myocarditis is markedly restricted in cells stimulated by specific antigen.
Wang
Yi-bin
YB
Children's Cardiovascular Diseases, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Hua
Yi-min
YM
Shi
Xiao-qing
XQ
Yang
Yuan
Y
Wei
Da-peng
DP
D000818:Animals; D000831:Animals, Newborn; D003384:Coxsackievirus Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D029822:Enterovirus B, Human / Q000254:growth & development; D019674:Genes, T-Cell Receptor beta / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism* / Q000821:virology