[Comparison of PCR methods for the diagnosis of congenital Trypanosoma cruzi infection].
PCR is a potentially interesting diagnostic tool to detect congenital T. cruzi infection. We have compared the sensitivity and capacity of a battery of T. cruzi PCR primers to detect the complete spectrum of known T. cruzi lineages, in order to improve and simplify the detection of infection in neonatal blood. We found that the primers Tcz1/Tcz2, targeting the 195 bp satellite repeat, detected all the parasitic lineages with the same sensitivity For all other tested primers (nDNA primers: BP1/BP2, 01/02, Pon1/ Pon2 and Tca1/Tca2; kDNA primers: S35VS36, 121/122), either, the intensity of amplicons varied according to T. cruzi lineages, or the assess were less sensitive. In order to better assess such PCR protocol, we assayed 311 samples of neonatal blood previously tested with parasitological methods. Reliability of our PCR test was demonstrated since all the 18 blood samples from newborns with congenital T. cruzi infection were positive, whereas the remaining samples (30 from control newborns of uninfected mothers and 262 out of 263 from babies, parasitologically negative, born from infected mothers) were negative. As our PCR method is simple, reliable, robust and cheap, it appears suitable for the detection of T. cruzi infection in neonatal blood.
Laboratoire de Chimie Biologique, Faculté de Médecine, Université Libre de Bruxelles, Brussels, Belgium.
Myrna
M
Virreira
Faustino
F
Torrico
Carine
C
Truyens
Cristina
C
Alonso-Vega
Marco
M
Solano
Yves
Y
Carlier
Michal
M
Svoboda
D000818:Animals; D014355:Chagas Disease / Q000151:congenital* / Q000175:diagnosis*; D017931:DNA Primers; D016054:DNA, Protozoan / Q000097:blood; D005312:Fetal Blood / Q000469:parasitology; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D016133:Polymerase Chain Reaction / Q000592:standards*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014349:Trypanosoma cruzi / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D016054:DNA, Protozoan