Sequencing and staphylococci identification.
The emerging clinical importance of staphylococcal infections prompted us to establish a reference database for partial RNA polymerase B (rpoB; nucleotides 1444-1928) gene sequences from type strains of all staphylococcal species and subspecies. This database correctly identified 55 clinical staphylococcal isolates; all were correctly identified at the species level. At the subspecies level, rpoB misidentified only 2 isolates.
University Hospital Münster, Münster, Germany.
Alexander
A
Mellmann
Karsten
K
Becker
Christof
C
von Eiff
Ursula
U
Keckevoet
Peter
P
Schumann
Dag
D
Harmsen
D000818:Animals; D015373:Bacterial Typing Techniques; D030541:Databases, Genetic; D006801:Humans; D012319:RNA Polymerase II / Q000235:genetics*; D012015:Reference Standards; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000235:genetics
D012319:RNA Polymerase II