[Fluorescence polarization used to derive cell membrane fluidity during photodynamic therapy].
With metal phthalocyanine used as the photosensitizer, the cell membrane fluidity under the conditions of different irradiation time in photodynamic therapy was investigated by fluorescence polarization. Results show that with the photosensitizer phthalocyanine excited, the fluorescence polarization of the fluorophose was increased and the cancer cell membrane fluidity was decreased due to the photodynamic therapy. Cell proliferation was also studied by MTT. Results show that cell membrane fluidity and cell proliferation are influenced to the same extent, suggesting that cell membrane is one of the binding sites in PDT.
Key Laboratory for Optical and Magnetic Resonance Spectroscopy, Department of Physics, East China Normal University, Shanghai 200062, China.
Rong-hui
RH
Wei
Yan-ping
YP
Huang
Shan-shan
SS
Li
Chun-yuan
CY
Qi
D001943:Breast Neoplasms / Q000188:drug therapy; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D007211:Indoles / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D054833:Isoindoles; D008560:Membrane Fluidity / Q000187:drug effects*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D013050:Spectrometry, Fluorescence / Q000379:methods*
D007211:Indoles; D054833:Isoindoles; D017319:Photosensitizing Agents; C013647:phthalocyanine