Collision-induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap-orbitrap mass spectrometry.
The yeast Saccharomyces cerevisiae synthesizes three classes of sphingolipids: inositolphosphoceramides (IPCs), mannosyl-inositolphosphoceramides (MIPCs), and mannosyl-diinositolphosphoceramides (M(IP)2C). Tandem mass spectrometry of their molecular anions on a hybrid quadrupole time-of-flight (QqTOF) instrument produced fragments of inositol-containing head groups, which were specific for each lipid class. MS(n) analysis performed on a hybrid linear ion trap-orbitrap (LTQ Orbitrap) mass spectrometer with better than 3 ppm mass accuracy identified fragment ions specific for the amide-linked fatty acid and the long chain base moieties in individual molecular species. By selecting m/z of class-specific fragment ions for multiple precursor ion scanning, we profiled yeast sphingolipids in total lipid extracts on a QqTOF mass spectrometer. Thus, a combination of QqTOF and LTQ Orbitrap mass spectrometry lends itself to rapid, comprehensive and structure-specific profiling of the molecular composition of sphingolipids and glycerophospholipids in important model organisms, such as fungi and plants.
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Christer S
CS
Ejsing
Thomas
T
Moehring
Ute
U
Bahr
Eva
E
Duchoslav
Michael
M
Karas
Kai
K
Simons
Andrej
A
Shevchenko
D020404:Glycerophospholipids / Q000032:analysis / Q000737:chemistry; D006028:Glycosphingolipids / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013107:Sphingolipids / Q000032:analysis* / Q000737:chemistry; D013110:Sphingosine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry
D020404:Glycerophospholipids; D006028:Glycosphingolipids; D013107:Sphingolipids; C025524:inositolphosphoceramides; C479822:mannosylinositol phosphoylceramide; D013110:Sphingosine; C005682:safingol