[Activity of the TNF-related apoptosis-inducing ligand gene expressed from the hTERT promoter on colon cancer cell line HT-29].
To evaluate the expression and activity of TNF-related apoptosis-inducing ligand (TRAIL) gene expressed from the hTERT promoter on colon cancer cell line HT-29.



GFP/TRAIL gene expressed from the hTERT promoter was transfected into HT-29 with adenoviral vectors system, expression and apoptosis inducing ability of GFP/TRAIL protein were determined with fluorescence-activated cell sorting (FACS) method.



The expression of GFP gene was 31.4 % and 67.0 % with either hTERT promoter or CMV promoter in DLD1 cells; GFP/TRAIL gene was able to inhibit cell growth (74.2%) and induce apoptosis (25.8%) of HT-29 cells. There was significant difference between Ad/hTERT-gTRAIL and the other two control groups (PBS and Ad/CMV-GFP, P<0.05).



The GFP/TRAIL gene with hTERT promoter transfected by adenoviral vector was successfully expressed in HT-29 cell, which can both inhibit cell growth and induce apoptosis of colon cancer cell line HT-29.
Chen
Bin
B
Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China. cbxs_002@163.com
He
Chao
C
Lao
Wei-feng
WF
Huang
Xue-feng
XF
Fang
Bing-liang
BL
D000256:Adenoviridae / Q000235:genetics; D017209:Apoptosis / Q000502:physiology*; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D019073:HT29 Cells; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000096:biosynthesis* / Q000235:genetics; D019098:Telomerase / Q000235:genetics*; D014407:Tumor Cells, Cultured
D053221:TNF-Related Apoptosis-Inducing Ligand; D049452:Green Fluorescent Proteins; C509186:TERT protein, human; D019098:Telomerase