GINS, a central nexus in the archaeal DNA replication fork.
In eukaryotes, the GINS complex is essential for DNA replication and has been implicated as having a role at the replication fork. This complex consists of four paralogous GINS subunits, Psf1, Psf2, Psf3 and Sld5. Here, we identify an archaeal GINS homologue as a direct interaction partner of the MCM helicase. The core archaeal GINS complex contains two subunits that are poorly conserved homologues of the eukaryotic GINS subunits, in complex with a protein containing a domain homologous to the DNA-binding domain of bacterial RecJ. Interaction studies show that archaeal GINS interacts directly with the heterodimeric core primase. Our data suggest that GINS is important in coordinating the architecture of the replication fork and provide a mechanism to couple progression of the MCM helicase on the leading strand with priming events on the lagging strand.
MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK.
Nina
N
Marinsek
Elizabeth R
ER
Barry
Kira S
KS
Makarova
Isabelle
I
Dionne
Eugene V
EV
Koonin
Stephen D
SD
Bell
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics*; D026341:Minichromosome Maintenance 1 Protein / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D048229:Sulfolobus solfataricus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
D019843:Archaeal Proteins; D002868:Chromosomal Proteins, Non-Histone; D026341:Minichromosome Maintenance 1 Protein; D046912:Multiprotein Complexes