Microarray profiling of lymphocytes in internal diseases with an altered immune response: potential and methodology.
Recently it has become possible to investigate expression of all human genes with microarray technique. The authors provide arguments to consider peripheral white blood cells and in particular lymphocytes as a model for the investigation of pathophysiology of asthma, RA, and SLE diseases in which inflammation is a major component. Lymphocytes are an alternative to tissue biopsies that are most often difficult to collect systematically. Lymphocytes express more than 75% of the human genome, and, being an important part of the immune system, they play a central role in the pathogenesis of asthma, RA, and SLE. Here we review alterations of gene expression in lymphocytes and methodological aspects of the microarray technique in these diseases. Lymphocytic genes may become activated because of a general nonspecific versus disease-specific mechanism. The authors suppose that in these diseases microarray profiles of gene expression in lymphocytes can be disease specific, rather than inflammation specific. Some potentials and pitfalls of the array technologies are discussed. Optimal clinical designs aimed to identify disease-specific genes are proposed. Lymphocytes can be explored for research, diagnostic, and possible treatment purposes in these diseases, but their precise value should be clarified in future investigation.
Department of Psychiatry, University Medical Center of Groningen, The Netherlands. a.v.gladkevich@psy.umcg.nl
Anatoliy
A
Gladkevich
S Adriaan
SA
Nelemans
Henk F
HF
Kauffman
Jakob
J
Korf
D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000276:immunology / Q000503:physiopathology; D001249:Asthma / Q000235:genetics / Q000276:immunology / Q000503:physiopathology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007107:Immune System / Q000502:physiology; D008180:Lupus Erythematosus, Systemic / Q000235:genetics / Q000276:immunology / Q000503:physiopathology; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*