Plasma prohepcidin positively correlates with hematocrit in chronic hemodialysis patients.
OBJECTIVE
Experimental studies have demonstrated interleukin-6 and iron load induce expression of hepcidin (an iron regulatory peptide), whereas anemia and erythropoietin (EPO) suppress its expression. We are the first to explore the relationships of plasma prohepcidin (the pro-hormone of hepcidin) in patients undergoing chronic hemodialysis (HD).


METHODS
We enrolled 71 chronic HD patients. During the preceding 3 months before enrollment, they all had steady weekly levels of haematocrit (Hct) and fixed subcutaneous doses of recombinant human EPO. Plasma levels of prohepcidin, proinflammatory cytokines, and EPO were determined by ELISA kits.


RESULTS
Of the patients, prohepcidin levels correlated positively with Hct, and negatively with interleukin-6 and EPO. Examined by a multivariate lineal regression method, we found Hct was the only significant predictor of plasma prohepcidin level. However, prohepcidin had no significant correlation with iron profiles.


CONCLUSIONS
Our findings suggest prohepcidin expression in chronic HD patients might be positively regulated by Hct.
Department of Internal Medicine, Far Eastern Memorial Hospital, Taipei, Taiwan.
Shih-Ping
SP
Hsu
Chih-Kang
CK
Chiang
Chiang-Ting
CT
Chien
Kuan-Yu
KY
Hung
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D023181:Antimicrobial Cationic Peptides / Q000097:blood*; D002908:Chronic Disease; D016207:Cytokines / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D004921:Erythropoietin / Q000008:administration & dosage / Q000097:blood; D005260:Female; D006400:Hematocrit / Q000379:methods*; D064451:Hepcidins; D006801:Humans; D007249:Inflammation / Q000097:blood; D007279:Injections, Subcutaneous; D015850:Interleukin-6 / Q000097:blood; D007501:Iron / Q000097:blood; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011498:Protein Precursors / Q000097:blood*; D011994:Recombinant Proteins / Q000008:administration & dosage; D006435:Renal Dialysis
D023181:Antimicrobial Cationic Peptides; D016207:Cytokines; D064451:Hepcidins; D015850:Interleukin-6; D011498:Protein Precursors; D011994:Recombinant Proteins; D004921:Erythropoietin; D007501:Iron