[Determination of residual sulfonamides in meat by high performance liquid chromatography with solid-phase extraction].
A method was developed for determining residual sulfonamides (SAs) such as sulfamethazine (SM2), sulfamonomethoxine (SMM), sulfamethiazole (SMZ), sulfadimethoxine (SDM) and sulfaquinoxaline (SQ) in pork and chicken using solid-phase extraction (SPE) and high performance liquid chromatography (HPLC) with a photodiode array detector. The samples were extracted with ethyl acetate. An NH2 column was used for clean up. For the HPLC determination, an Intersil ODS-2 column was used with a mixture of methanol-acetonitrile-water-acetic acid (2: 2: 9: 0.2, v/v) as the mobile phase. The detection limits (S/N = 3) were 3 microg/kg for SM2, SMM and SMZ, and 7 microg/kg for SDM and SQ. The quantitation limits (S/N = 10) were 10 microg/kg for SM2, SMM and SMZ, and 25 microg/kg for SDM and SQ. The linear ranges were 30 - 5 000 microg/L for SM2, SMM and SMZ, and 60 - 5 000 microg/L for SDM and SQ. The recoveries were between 73.2% and 97.3% with the relative standard deviations between 2.5% and 11.6% originated from the spiked level of 50 microg/kg.
Zhang
Yan
Y
College of Science, China Agricultural University, Beijing 100094, China. zhangyun1979@sohu.com
Wu
Yinliang
Y
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013412:Sulfadimethoxine / Q000032:analysis / Q000737:chemistry; D013418:Sulfamethazine / Q000032:analysis / Q000737:chemistry; D013419:Sulfamethizole / Q000032:analysis / Q000737:chemistry; D013422:Sulfamonomethoxine / Q000032:analysis / Q000737:chemistry; D013428:Sulfaquinoxaline / Q000032:analysis / Q000737:chemistry; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry; D013552:Swine
D013449:Sulfonamides; D013419:Sulfamethizole; D013412:Sulfadimethoxine; D013418:Sulfamethazine; D013422:Sulfamonomethoxine; D013428:Sulfaquinoxaline