Preproghrelin Leu72Met polymorphism is not associated with type 2 diabetes mellitus.
Ghrelin is a novel gut-brain peptide, which exerts somatotropic, orexigenic, and adipogenic effects. Genetic variants of ghrelin have been associated with both obesity and insulin metabolism. In this study, we determined a role of preproghrelin Leu72Met polymorphism on type 2 diabetes mellitus and its relationship to variables studied. Genotypes were assessed by polymerase chain reaction. Frequencies of the Leu72Met polymorphism were found to be 35.4% in the type 2 diabetic patients and 32.5% in the normal controls. The Leu72Met polymorphism was not associated with hypertension, macroangiopathy, retinopathy, serum cholesterol, triglyceride, blood urea nitrogen, HbA(1c), lipoprotein (a), fasting insulin, or 24-hour urinary protein levels in the type 2 diabetic group. However, the Leu72Met polymorphism was clearly associated with serum creatinine levels in the diabetic group, as the Met72 carriers exhibited lower serum creatinine levels than the Met72 noncarriers. Our data indicate that the preproghrelin Leu72Met polymorphism is not associated with type 2 diabetes mellitus. However, the Leu72Met polymorphism is associated with serum creatinine levels. These data suggest that Met72 carrier status may be a predictable marker for diabetic nephropathy or renal impairment in type 2 diabetes mellitus.
Department of Pediatrics, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-712, Korea.
Sun-Young
SY
Kim
Dae-Sun
DS
Jo
Pyoung Han
PH
Hwang
Ji Hyun
JH
Park
Sung Kwang
SK
Park
Ho Keun
HK
Yi
Dae-Yeol
DY
Lee
D000368:Aged; D016022:Case-Control Studies; D003404:Creatinine / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D054439:Ghrelin; D006801:Humans; D008297:Male; D008875:Middle Aged; D009037:Motilin / Q000235:genetics*; D020125:Mutation, Missense; D011110:Polymorphism, Genetic
D054439:Ghrelin; D009037:Motilin; D003404:Creatinine