Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs.
MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. By using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target messenger RNA molecules (mRNAs). Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.
Giraldez
Antonio J
AJ
Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA. giraldez@mcb.harvard.edu
Mishima
Yuichiro
Y
Rihel
Jason
J
Grocock
Russell J
RJ
Van Dongen
Stijn
S
Inoue
Kunio
K
Enright
Anton J
AJ
Schier
Alexander F
AF
D020413:3' Untranslated Regions; D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000502:physiology*; D047108:Embryonic Development; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000502:physiology*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D011108:Polymers / Q000378:metabolism*; D014176:Protein Biosynthesis; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D015854:Up-Regulation; D015027:Zebrafish / Q000196:embryology; D015053:Zygote / Q000502:physiology
D020413:3' Untranslated Regions; D035683:MicroRNAs; D011108:Polymers; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; C069424:polyadenosine; D043244:Ribonuclease III; D000241:Adenosine