[Effects of the Radix Ranuncoli Ternati extracts on Mycobacterium tuberculosis proteome profiling revealed by 2D electrophoresis].
Radix Ranuncoli Ternati is clinically effective traditional Chinese medicine for multidrug resistant tuberculosis. Its active components and mechanism of action remain unsolved. Two dimensional gel electrophoresis (2-DE) was employed to address this problem. Globlal proteome of Mycobacterium tuberculosis untreated and treated with Radix Ranuncoli Ternati were compared, and 22 protein spots were found to be expressed differentially. 3 protein spots which remarkably decreased in Mycobacterium tuberculosis treated with Radix Ranuncoli Ternati were subjected to matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) analysis. The data obtained from peptide mass finger printing were used for database search. The 3 protein spots in gel were identified as cysA2 (thiosulfate sulfurtransferase), tsf (elongation factor EF-Ts) and hspX (heat shock protein X). These data provide insights into the changed global protein patterns of Mycobacterium tuberculosis treated with Radix Ranuncoli Ternati and may prove useful for further study in the mechanism in how Radix Ranuncoli Ternati influence the life of Mycobacterium tuberculosis. The differentially expressed proteins may be potential novel antituberculosis drug targets.
Institute of Modern Biopharmaceuticals, School of Life Sciences, Southwest China University, Chongqing 400715, China. heying8080@sohu.com
Ying
Y
He
Jun
J
Yue
Chang-Hua
CH
Hu
Hong-Hai
HH
Wang
Jian-Ping
JP
Xie
Cui
C
Tang
Xiu-Li
XL
Wei
Xue-Mei
XM
Liu
Jie
J
Song
D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D008516:Medicine, Chinese Traditional; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000187:drug effects*; D010444:Peptide Elongation Factor Tu / Q000032:analysis; D010445:Peptide Elongation Factors / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C118127:HspX protein, Mycobacterium tuberculosis; D010445:Peptide Elongation Factors; D010936:Plant Extracts; D020543:Proteome; C023512:elongation factor Ts; D010444:Peptide Elongation Factor Tu