SDS-PAGE and Western blotting to detect proteins and glycoproteins of interest in breast cancer research.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting to detect proteins and glycoproteins is one of the most widely used and broadly useful techniques in cancer research, allowing the proteins in a complex sample--such as a blood sample, aspirate, or solid tumor homogenate--to be separated according to molecular weight and visualized within a gel matrix and/or, once separated, transferred onto a supporting membrane, where they may be probed for the binding of antibodies or lectins. In this chapter, the theory and principles of SDS-PAGE and Western blotting are briefly outlined, and basic methods are given that can be applied to investigate virtually any (glyco)protein of interest in breast cancer research.
Osborne
Chloe
C
Department of Physiology, University of Birmingham Medical School, UK.
Brooks
Susan A
SA
D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western / Q000379:methods*; D001943:Breast Neoplasms / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*
D014408:Biomarkers, Tumor; D006023:Glycoproteins; D009363:Neoplasm Proteins