LCD-based detection of enzymatic action.
By incorporating an ester-containing substrate in a self-assembled alignment layer for liquid crystal cells, the presence of a lipase (CALB) can be directly detected through its enzymatic action on the alignment layer, without the need for fluorescent labelling or enzyme assays.
Hoogboom
Johan
J
Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525ED, Nijmegen, The Netherlands. J.Hoogboom@science.ru.nl
Velonia
Kelly
K
Rasing
Theo
T
Rowan
Alan E
AE
Nolte
Roeland J M
RJ
D015374:Biosensing Techniques / Q000379:methods*; D002175:Candida / Q000201:enzymology*; D004789:Enzyme Activation; D005656:Fungal Proteins; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D050866:Liquid Crystals / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D013379:Substrate Specificity; D017971:Tin Compounds / Q000737:chemistry
D005656:Fungal Proteins; D011437:Propylamines; D012821:Silanes; D017971:Tin Compounds; C109984:indium tin oxide; D008049:Lipase; C431349:lipase B, Candida antarctica; C477625:amino-propyl-triethoxysilane