Effects of antioxidant therapy on leukocyte myeloperoxidase and Cu/Zn-superoxide dismutase and plasma malondialdehyde levels in experimental colitis.
The aim of the present study was to evaluate the effects of N-acetylcysteine (NAC) and L-carnitine (LCAR) supplementations on polymorphonuclear leukocytes myeloperoxidase (MPO) and Cu/Zn-superoxide dismutase (Cu/Zn-SOD) and plasma malondialdehyde (MDA) in acetic acid (AA)-induced ulcerative colitis model. The mean polymorphonuclear leukocyte MPO and Cu/Zn-SOD activity was significantly higher in the colitis group than in the control group. Both NAC and LCAR pretreatment markedly decreased MPO and Cu/Zn-SOD activity compared to colitis group. AA administration significantly increased the levels of plasma MDA in comparison with controls. However, NAC and LCAR administration to the AA-treated rats significantly reduced the MDA levels compared to colitis group. In conclusion NAC and LCAR could be beneficial agents in restoring the circulating proinflammatory mediators.
Department of Biochemistry, Medical Faculty, Sutcu Imam University, Turkey.
Ergul Belge
EB
Kurutas
Ali
A
Cetinkaya
Ertan
E
Bulbuloglu
Bulent
B
Kantarceken
D000111:Acetylcysteine / Q000627:therapeutic use*; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D002331:Carnitine / Q000627:therapeutic use*; D003093:Colitis, Ulcerative / Q000097:blood / Q000188:drug therapy / Q000201:enzymology; D016166:Free Radical Scavengers / Q000627:therapeutic use; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D009195:Peroxidase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*; D014803:Vitamin B Complex / Q000627:therapeutic use
D000975:Antioxidants; D016166:Free Radical Scavengers; D014803:Vitamin B Complex; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase; D002331:Carnitine; D000111:Acetylcysteine