Investigation of the mechanism of action of 3-(4-bromophenyl)-5-acyloxymethyl-2,5-dihydrofuran-2-one against Candida albicans by flow cytometry.
The mechanism of action of the antifungal agent 3-(4-bromophenyl)-5-acyloxymethyl-2,5-dihydrofuran-2-one against Candida albicans was investigated by flow cytometry, using propidium iodide, DiBAC4(3), and FUN-1 as the fluorescent dyes. A related but less active agent, together with amphotericin B and fluconazole, was tested in parallel for comparison of the results. The incrustoporine derivative was found to have a potent fungicidal activity on C. albicans, resulting in damage of cell membrane.
Vale-Silva
Luís A
LA
Department of Biological and Medical Sciences, School of Pharmacy, Charles University, Heyrovského 1203, CZ-500 05 Hradec Králové, Czech Republic. luisandrevale.silva@faf.cuni.cz
Buchta
Vladimír
V
Vokurková
Doris
D
Pour
Milan
M
D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D005434:Flow Cytometry / Q000379:methods*; D005663:Furans / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D013329:Structure-Activity Relationship
C511140:3-(4-bromophenyl)-5-acyloxymethyl-2,5-dihydrofuran-2-one; D000935:Antifungal Agents; D005663:Furans