Transient development of filamentous Thiothrix species in a marine sulfide oxidizing, denitrifying fluidized bed reactor.
In this study, microscopic and molecular microbial analyses were integrated to characterize rapidly developing white filamentous tufts in a fluidized bed reactor used for nitrate removal from a marine recirculating fish culture system. Formation and rapid elongation of the tufts (often exceeding 50 mm day (-1)) was strongly correlated to transient elevated sulfide concentrations (>50 microM) in the reactor. The dominant bacterial constituents of these tufts were filamentous gram-negative bacteria with densely packed intracellular sulfur granules. Using 16S rRNA gene analysis and fluorescence in situ hybridization it was found that these filamentous bacteria represented a novel Thiothrix phylotype closely related (97% sequence identity) to a previously identified Thiothrix strain endogenous to the marine crustacean Urothoe poseidonis. In addition to filamentous morphotypes, rosette-shaped morphotypes of Thiothrix were also detectable within the tufts.
Department of Animal Sciences, The Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel.
Eddie
E
Cytryn
Dror
D
Minz
Armin
A
Gieseke
Jaap
J
van Rijn
D001483:Base Sequence; D019149:Bioreactors / Q000382:microbiology*; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007220:Industrial Waste; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D017422:Sequence Analysis, DNA; D013440:Sulfides / Q000032:analysis / Q000378:metabolism; D053525:Thiothrix / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D014871:Water Microbiology
D017931:DNA Primers; D015342:DNA Probes; D004269:DNA, Bacterial; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; D009584:Nitrogen