[Determination of apomorphine, sildenafil and alprostadil in medicines for erectile dysfunction by high performance liquid chromatography-mass spectrometry].
A high performance liquid chromatography-mass spectrometry (LC-MS) analytical method for illicit drugs, apomorphine, sildenafil and alprostadil, in medicines for erectile dysfunction has been developed. The samples were extracted with methanol using ultrasound-assisted extraction. The chromatographic separation was performed on a Zorbax Eclipse XDB-C18 column using acetonitrile-0.5% formic acid aqueous solution as mobile phase. The three compounds were identified by retention time and m/z and quantified by peak area. The results demonstrated that the linear ranges were 50.0 - 5 000.0 microg/L, 10.0 - 1 000.0 microg/L, 40.0 - 4 000.0 microg/L, with detection limits of 20.0, 4.0, 10.0 microg/L for apomorphine, sildenafil and alprostadil, respectively. The average recoveries and the relative standard deviations were 89% - 95% and 9.5% - 11%. The method is simple, rapid, accurate and suitable for the simultaneous determination of these drugs in medicines for erectile dysfunction.
Xu
Yuanjin
Y
Engineering Center for Sugar Cane and Cane Sugar, Guangxi University, Nanning 530004, China. yjxu@gxu.edu.cn
Xu
Guiping
G
D000527:Alprostadil / Q000032:analysis* / Q000627:therapeutic use; D001058:Apomorphine / Q000032:analysis* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018491:Dopamine Agonists / Q000032:analysis / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000627:therapeutic use; D007172:Erectile Dysfunction / Q000188:drug therapy*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D010726:Phosphodiesterase Inhibitors / Q000032:analysis / Q000627:therapeutic use; D010879:Piperazines / Q000032:analysis* / Q000627:therapeutic use; D011687:Purines / Q000032:analysis / Q000627:therapeutic use; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000032:analysis* / Q000627:therapeutic use
D018491:Dopamine Agonists; D004365:Drugs, Chinese Herbal; D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D000068677:Sildenafil Citrate; D000527:Alprostadil; D001058:Apomorphine