Expression of recombinant Streptolysin O and specific antibody production.
Streptolysin O (SLO), an oxygen-labile cytolysin, is the cholesterol-binding exotoxin of hemolytic streptococci. Besides microbiological and pathological interests, this cytolysin has been used as a tool for permeabilization of biomembranes. SLO serves as a diagnostic reagent for determination of anti-SLO antibody titer in streptococcal infection. Availability of highly purified SLO, however, has been limited by low yield in streptococcal culture and purification process. Present subcloning of mature-type full-length SLO gene into an expression vector having strictly controllable araBAD promoter enabled efficient production of the cytolysin. Further, anti-SLO antibody with high specificity was obtained by immunizing with purified SLO protein.
Kimoto
Hisashi
H
Department of Molecular Genetics, Fukui University, Matsuoka, Japan. hisashi@fmsrsa.fukui-med.ac.jp
Fujii
Yutaka
Y
Hirano
Satoko
S
Yokota
Yoshifumi
Y
Taketo
Akira
A
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification; D013291:Streptococcus / Q000235:genetics / Q000378:metabolism*; D013301:Streptolysins / Q000276:immunology / Q000302:isolation & purification*
D000906:Antibodies; D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D013301:Streptolysins; C025964:streptolysin O