Communication between accessory factors and the Cre recombinase at hybrid psi-loxP sites.
By placing loxP adjacent to the accessory sequences from the Xer/psi multimer resolution system, we have imposed topological selectivity and specificity on Cre/loxP recombination. In this hybrid recombination system, the Xer accessory protein PepA binds to psi accessory sequences, interwraps them, and brings the loxP sites together such that the product of recombination is a four-node catenane. Here, we investigate communication between PepA and Cre by varying the distance between loxP and the accessory sequences, and by altering the orientation of loxP. The yield of four-node catenane and the efficiency of recombination in the presence of PepA varied with the helical phase of loxP with respect to the accessory sequences. When the orientation of loxP was reversed, or when half a helical turn was added between the accessory sequences and loxP, PepA reversed the preferred order of strand exchange by Cre at loxP. The results imply that PepA and the accessory sequences define precisely the geometry of the synapse formed by the loxP sites, and that this overcomes the innate preference of Cre to initiate recombination on the bottom strand of loxP. Further analysis of our results demonstrates that PepA can stimulate strand exchange by Cre in two distinct synaptic complexes, with the C-terminal domains of Cre facing either towards or away from PepA. Thus, no specific PepA-recombinase interaction is required, and correct juxtaposition of the loxP sites is sufficient to activate Cre in this system.
Akopian
Aram
A
Division of Molecular Genetics, Institute of Biomedical and Life Sciences, Anderson College, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, UK.
Gourlay
Sarah
S
James
Helen
H
Colloms
Sean D
SD
D000818:Animals; D001483:Base Sequence; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D004996:Ethidium / Q000378:metabolism; D007202:Indicators and Reagents / Q000378:metabolism; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D029968:Escherichia coli Proteins; D007202:Indicators and Reagents; C496211:PepA protein, E coli; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases; D004996:Ethidium