The identification and characterization of xenoantigenic nonhuman carbohydrate sequences in membrane proteins from porcine kidney.
The immunogenic nonhuman carbohydrate sequences in membrane proteins from porcine kidney were identified and characterized using MALDI-TOF MS and ESI-QTOF-MS. The MALDI profile, investigated by incubation with exoglycosidases, showed a series of about 40 carbohydrates that were identified as high mannose glycans (Man(3-9)GlcNAc2) and complex bi-, tri-, and tetra-antennary glycans with and without core fucose. The antennae of many of the complex glycans were terminated with alpha-galactose residues, with the numbers of these residues ranging from one up to the number of antennae. Negative ion ESI-MS/MS spectra confirmed the location of the alpha-galactose residues on the ends of the antennae. This total glycan profile of the membrane proteins from porcine kidney will thus provide important information for the study of molecular interactions between antigenic carbohydrates and proteins in xenotransplantation.
Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, Seoul, Korea.
Yun-Gon
YG
Kim
Sun-Young
SY
Kim
Young-Mi
YM
Hur
Hwang-Soo
HS
Joo
Junho
J
Chung
Dong-Sup
DS
Lee
Louise
L
Royle
Pauline M
PM
Rudd
Raymond A
RA
Dwek
David J
DJ
Harvey
Byung-Gee
BG
Kim
D000818:Animals; D002240:Carbohydrate Sequence; D006020:Glycopeptides / Q000032:analysis; D006026:Glycoside Hydrolases / Q000378:metabolism; D007519:Isoantigens / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine
D006020:Glycopeptides; D007519:Isoantigens; D008565:Membrane Proteins; D006026:Glycoside Hydrolases