Brain-specific change in alternative splicing of Tau exon 6 in myotonic dystrophy type 1.
Alternative splicing is altered in myotonic dystrophy of type 1 (DM1), a syndrome caused by an increase of CTG triplet repeats in the 3' untranslated region of the myotonic dystrophy protein kinase gene. Previously, we reported the preferential skipping of Tau exon 2 in DM1 brains. In this study, we analyze the alternative splicing of Tau exon 6 which can be inserted in three different forms (c, p and d) depending on the 3' splice site used. In fact, inclusion of exon 6c decreases in DM1 brains compared to control brains whereas inclusion of 6d increases. Alteration of exon 6 splicing was not observed in DM1 muscle although this exon was inserted in RNAs from normal muscle and DM1 splicing alterations were first described in this organ. In contrast, alteration of exon 2 of Tau mRNA was observed in both muscle and brain. However, co-transfections of a minigene containing exon 6 with CELF or MBNL1 cDNAs, two splicing factor families suspected to be involved in DM1, showed that they influence exon 6 splicing. Altogether, these results show the importance of determining all the exons and organs targeted by mis-splicing to determine the dysregulation mechanisms of mis-splicing in DM1.
INSERM U422, Pl. de Verdun. 59045 Lille Cedex- France.
Olivier
O
Leroy
Junning
J
Wang
Claude-Alain
CA
Maurage
Michel
M
Parent
Thomas
T
Cooper
Luc
L
Buée
Nicolas
N
Sergeant
Athena
A
Andreadis
Marie-Laure
ML
Caillet-Boudin
D017398:Alternative Splicing; D001921:Brain / Q000378:metabolism*; D051743:CCAAT-Enhancer-Binding Protein-delta / Q000378:metabolism; D002460:Cell Line; D005091:Exons; D006801:Humans; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism; D009223:Myotonic Dystrophy / Q000235:genetics* / Q000378:metabolism; D065636:Myotonin-Protein Kinase; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D016875:tau Proteins
C497252:CEBPD protein, human; C585517:DMPK protein, human; C054369:MAPT protein, human; C493663:MBNL1 protein, human; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; D016875:tau Proteins; D051743:CCAAT-Enhancer-Binding Protein-delta; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases