Formation of singlet oxygen from solutions of vitamin E.
Vitamin E offers protection against oxidative stress and is an efficient quencher of singlet oxygen. A recent report suggests that photo-excitation of vitamin E results in the formation of a triplet state (Naqvi et al. Photochem Photobiol Sci 2, 381 (2003)). This leads to the possibility of the triplet state of vitamin E being able to sensitize singlet oxygen and if this is the case it would be counter productive in terms of the biological protective function of vitamin E. We report the production of singlet oxygen, detected by 1270 nm luminescence, from pulsed laser excitation (308 nm) of vitamin E and an analogue, 2,2,5,7,8-pentamethyl-6-hydroxy-chroman (PMHC), with quantum yields between ~0.1 and 0.2. The luminescence was identified as singlet oxygen from self-quenching by vitamin E with solvent-dependent rate constants similar to published values. Whilst the beneficial antioxidant aspects of vitamin E are well established, these results indicate that vitamin E when directly excited can sensitize singlet oxygen formation and may, therefore, be capable of inducing biochemical and biological damage. The results are discussed in relation to recent reports on the deleterious effects of vitamin E dietary supplementation and pro-oxidant effects of vitamin E.
Dad
Shakeela
S
Biosciences Research Institute, University of Salford, Salford M5 4WT, UK.
Bisby
Roger H
RH
Clark
Ian P
IP
Parker
Anthony W
AW
D000975:Antioxidants / Q000378:metabolism*; D026082:Singlet Oxygen / Q000378:metabolism*; D012996:Solutions; D014810:Vitamin E / Q000378:metabolism*
D000975:Antioxidants; D012996:Solutions; D014810:Vitamin E; D026082:Singlet Oxygen