[RAPD analysis of plant pathogenic coryneform bacteria].
RAPD analysis was used for the taxonomy of plant pathogenic coryneform bacteria, especially for the classification of two new pathogens (Curtobacterium flaccumfaciens pv. basellae pv. nov. and Curtobacterium flaccumfaciens pv. beticola pv. nov.). 20 random primers were screened from 50 ones to detect polymorphism among the total strains used. 80.4% were polymorphic bands among the 225 ones produced. The results of pairwise similarity and UPGMA cluster analysis suggest that the two new pathovars of sugar beet (Beta vulgaris var. saccharifera) and malabar spinach (Basella rubra) are genetically close related with Curtobacterium flacumfaciens, and the minimal similarity coefficient is 0.6511. According to the RAPD analysis and previous research, some newly made taxonomic changes of the plant pathogenic coryneform bacteria are discussed.
Department of Plant Pathology, College of Plant Protection, Nanjing Agriculture University, Nanjing 210095, China. ynyin2003@yahoo.com.cn
Yan-Ni
YN
Yin
Yong-Fang
YF
Chen
Shi-Mo
SM
Li
Jian-Hua
JH
Guo
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics; D010944:Plants / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*