Regulation of carotenoid and ABA accumulation during the development and germination of Nicotiana plumbaginifolia seeds.
Abscisic acid (ABA) is derived from epoxycarotenoid cleavage and regulates seed development and maturation. A detailed carotenoid analysis was undertaken to study the contribution of epoxycarotenoid synthesis to the regulation of ABA accumulation in Nicotiana plumbaginifolia developing seeds. Maximal accumulation of xanthophylls occurred at mid-development in wild type seeds, when total ABA levels also peaked. In contrast, in ABA-deficient mutants xanthophyll synthesis was delayed, in agreement with the retardation in seed maturation. Seed dormancy was restored in mutants impaired in the conversion of zeaxanthin into violaxanthin by zeaxanthin epoxidase (ZEP), by the introduction of the Arabidopsis AtZEP gene under the control of promoters inducing expression during later stages of seed development compared to wild type NpZEP, and in dry and imbibed seeds. Alterations in the timing and level of ZEP expression did not highly affect the temporal regulation of ABA accumulation in transgenic seeds, despite notable perturbations in xanthophyll accumulation. Therefore, major regulatory control of ABA accumulation might occur downstream of epoxycarotenoid synthesis.
INRA-INAPG, Institut Jean-Pierre Bourgin, UMR204, Laboratoire de Biologie des Semences, 78026 Versailles Cedex, France.
Anne
A
Frey
Jean-Pierre
JP
Boutin
Bruno
B
Sotta
Raphaël
R
Mercier
Annie
A
Marion-Poll
D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018525:Germination; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000196:embryology / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D012639:Seeds / Q000254:growth & development / Q000378:metabolism*; D014026:Nicotiana / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D024341:Xanthophylls / Q000378:metabolism*
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D024341:Xanthophylls; D000040:Abscisic Acid; D010088:Oxidoreductases; C097153:zeaxanthin epoxidase