Analysis of the wheat and Puccinia triticina (leaf rust) proteomes during a susceptible host-pathogen interaction.
Wheat leaf rust is caused by the fungus Puccinia triticina. The genetics of resistance follows the gene-for-gene hypothesis, and thus the presence or absence of a single host resistance gene renders a plant resistant or susceptible to a leaf rust race bearing the corresponding avirulence gene. To investigate some of the changes in the proteomes of both host and pathogen during disease development, a susceptible line of wheat infected with a virulent race of leaf rust were compared to mock-inoculated wheat using 2-DE (with IEF pH 4-8) and MS. Up-regulated protein spots were excised and analyzed by MALDI-QqTOF MS/MS, followed by cross-species protein identification. Where possible MS/MS spectra were matched to homologous proteins in the NCBI database or to fungal ESTs encoding putative proteins. Searching was done using the MASCOT search engine. Remaining unmatched spectra were then sequenced de novo and queried against the NCBInr database using the BLAST and MS BLAST tools. A total of 32 consistently up-regulated proteins were examined from the gels representing the 9-day post-infection proteome in susceptible plants. Of these 7 are host proteins, 22 are fungal proteins of known or hypothetical function and 3 are unknown proteins of putative fungal origin.
Rampitsch
Christof
C
Agriculture and Agrifood Canada, Winnipeg, MB, Canada. crampitsch@agr.gc.ca
Bykova
Natalia V
NV
McCallum
Brent
B
Beimcik
Eva
E
Ens
Werner
W
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000472:pathogenicity*; D016208:Databases, Factual; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000032:analysis / Q000302:isolation & purification; D015966:Gene Expression Regulation, Fungal; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*; D015854:Up-Regulation
D005656:Fungal Proteins; D010940:Plant Proteins; D020543:Proteome