Evaluation of a series of new 99mTc-labeled bombesin-like peptides for early cancer detection.
Bombesin (BN) is a peptide exhibiting a high affinity for the gastrin-releasing peptide (GRP) receptor, which is overexpressed by a variety of tumors, including breast or prostate cancer. The aim of the present study was the investigation of the complexes formed between a series of BN-like peptides and the nuclides (185/187)Re and 99mTc. The (185/187)Re complexes were formed via the precursor Regluconate. The radiolabeling of the derivatives with 99mTc was performed using either 99mTc-gluconate or 99mTc-MDP as the intermediate complex. For the in vitro evaluation of the new peptides, the cancer cell line PC3 was used. The in vivo behavior of the 99mTc-labeled BN-like peptides was evaluated in normal mice. All the derivatives showed specific uptake in the pancreas, an organ rich in BN receptors and high affinity for the cancer cell line PC3. The above preliminary results indicated that the new BN derivatives are promising for human cancer studies.
Gourni
Eleni
E
Institute of Radioisotopes and Radiodiagnostic Products, National Center for Scientific Research Demokritos, Athens, Greece.
Paravatou
Maria
M
Bouziotis
Penelope
P
Zikos
Christos
C
Fani
Melpomeni
M
Xanthopoulos
Stavros
S
Archimandritis
Spyridon C
SC
Livaniou
Evagelia
E
Varvarigou
Alexandra D
AD
D000230:Adenocarcinoma / Q000000981:diagnostic imaging / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D001839:Bombesin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000493:pharmacokinetics; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000493:pharmacokinetics*; D012211:Rhenium / Q000737:chemistry / Q000493:pharmacokinetics; D017556:Technetium Compounds / Q000737:chemistry / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; D017556:Technetium Compounds; D012211:Rhenium; D001839:Bombesin