Carbon disulfide-induced changes in cytoskeleton protein content of rat cerebral cortex.
To investigate the mechanism of carbon disulfide-induced neuropathy, male wistar rats were administrated by gavage at dosage of 300 or 500 mg/kg carbon disulfide, five times per week for 12 weeks. By the end of the exposure, the animals produced a slight or moderate level of neurological deficits, respectively. Cerebrums of carbon disulfide-intoxicated rats and their age-matched controls were Triton-extracted and centrifuged at a high speed (100,000 x g) to yield a pellet fraction of NF polymer and a corresponding supernatant fraction, which presumably contained mobile monomer. Then, the contents of six cytoskeletal protein (NF-L, NF-M, NF-H, alpha-tubulin, beta-tubulin, and beta-actin) in both fractions were determined by immunoblotting. Results showed that the contents of the three neurofilament subunits in the pellet and the supernatant fraction decreased significantly regardless of dose levels (P<0.01). As for microtubule proteins, in the pellet fraction of cerebrum, the levels of alpha-tubulin and beta-tubulin demonstrated some inconsistent changes. However, in the supernatant fractions, the content of alpha-tubulin and beta-tubulin increased significantly in both two dose groups (P<0.01). In comparison to neurofilament and tubulin proteins, the content of beta-actin changed less markedly, only the supernatant fraction of the high dose group displayed significant increase (P<0.01), but the others remained unaffected. These findings suggested that the changes of cytoskeleton protein contents in rat cerebrum were associated with the intoxication of carbon disulfide, which might be involved in the development of carbon disulfide neurotoxicity.
Institute of Toxicology, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, P. R. China.
Fuyong
F
Song
Sufang
S
Yu
Xiulan
X
Zhao
Cuili
C
Zhang
Keqin
K
Xie
D000199:Actins / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D002246:Carbon Disulfide / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D008297:Male; D016900:Neurofilament Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D003598:Cytoskeletal Proteins; D016900:Neurofilament Proteins; D014404:Tubulin; D002246:Carbon Disulfide