Intrauterine transfer of early canine embryos.
The success rate of in vitro fertilization (IVF) in dogs is low and only early embryos have been obtained. In the present study, we investigated the use of intrauterine transfer of early-stage canine embryos to obtain pups. Twenty-three female dogs, in which the date of ovulation (based on plasma progesterone concentrations) differed by +/-1 day, were used (10 donors and 13 recipients). The uterine tube was extirpated under general anesthesia 1-4 days after mating (5-7 days after ovulation), and descending perfusion was done to collect embryos. Embryos were examined and transferred into the uterine horn of a recipient, ipsilateral to the ovary with the most corpora lutea. Pregnancy was established in one of eight bitches that received early embryos (zygote to 4-cell embryos); she received two zygotes and one 2-cell embryo and delivered two puppies. Although intrauterine transfer of early embryos (zygote to 4-cell embryos) was difficult, pregnancy was achieved, suggesting that uterine tube transfer is appropriate for these early-stage embryos.
Tsutsui
Toshihiko
T
Department of Reproduction, Nippon Veterinary and Animal Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan. tsusui@nvau.ac.jp
Hori
Tatsuya
T
Endo
Shinobu
S
Hayama
Arata
A
Kawakami
Eiichi
E
D000818:Animals; D004285:Dogs / Q000502:physiology*; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D008297:Male; D011247:Pregnancy