Influence of chromatin and single strand binding proteins on the activity of an archaeal MCM.
The mini-chromosome maintenance (MCM) complex is the presumptive replicative helicase in archaea and eukaryotes. In archaea, the MCM is a homo-multimer, in eukaryotes a heterohexamer composed of six related subunits, MCM 2-7. Biochemical studies using naked DNA templates have revealed that archaeal MCMs and a sub-complex of eukaryotic MCM 4, 6 and 7 have 3' to 5' helicase activity. Here, we investigate the influence of the major chromatin proteins, Alba and Sul7d, of Sulfolobus solfataricus (Sso) on the ability of the MCM complex to melt partial duplex DNA substrates. In addition, we test the effect of Sso SSB on MCM activity. We reveal that Alba represents a formidable barrier to MCM activity and further demonstrate that acetylation of Alba alleviates repression of MCM activity.
MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK.
Victoria L
VL
Marsh
Adam T
AT
McGeoch
Stephen D
SD
Bell
D000107:Acetylation; D019843:Archaeal Proteins / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D004265:DNA Helicases / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism*; D009691:Nucleic Acid Denaturation; D048229:Sulfolobus solfataricus / Q000235:genetics / Q000378:metabolism*
D019843:Archaeal Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; C444360:Sso10b protein, Sulfolobus solfataricus; D004265:DNA Helicases