Expression and properties of the Rab4, Rabaptin-5alpha, AP-1 complex in endosomal recycling.
We previously showed that the small GTPase Rab4 regulates formation of recycling vesicles from early endosomes. To understand how Rab4 accomplishes this task, we started to identify the Rab4 effector protein network. In this chapter, we describe experiments leading to the characterization of a complex consisting of Rab4GTP, its effector Rabaptin-5alpha, and the adaptor protein complex AP-1, which regulates recycling from endosomes.
Popa
Ioana
I
Deneka
Magda
M
van der Sluijs
Peter
P
D000818:Animals; D001921:Brain / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013552:Swine; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*; D020695:rab4 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
C097227:RABEP1 protein, human; D011994:Recombinant Proteins; D018808:Transcription Factor AP-1; D033921:Vesicular Transport Proteins; D020695:rab4 GTP-Binding Proteins