[Effects of acidic oligose on differentially expressed genes in the mice model of Alzheimer's disease by microarray].
OBJECTIVE
To investigate the molecular mechanism of protective effect of acidic oligose 971 on Alzheimer's disease mouse model by using microarray.


METHODS
Balb/c mice were randomly divided into control group, beta-AP(25-35) i.c.v. injected group and 971-treated group. The learning-memory ability of mice was tested by Morris water maze experiment. Total RNA of the cerebral cortex was extracted from the mice of each group. cDNA microarrays containing 1176 genes were used to investigate the gene expression pattern of each group. Expressions of 5 genes were randomly selected for further confirmation by RT-PCR.


RESULTS
Icv injection of beta-AP(25-35) caused significant impairments in spatial and working memory performances of mice in Morris water maze and which were relieved by the treatment of 971. Up- and down- regulated genes were 19 and 12 in beta-AP(25-35)-injected group vs control group, respectively. Up- and down- regulated genes were 13 and 4, respectively, in 971-treated group vs beta-AP(25-35)-injected group. RT-PCR results indicated that 5 genes showed identical results to that of the microarray.


CONCLUSIONS
The protective effect of 971 on learning and memory ability of beta-AP(25-35)-treated mouse may be related to the expression changes of genes involved in cell cycle, DNA repair, nerve growth, synaptic plasticity and immune response, etc.
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China.
Ling-na
LN
Kong
Mei-yu
MY
Geng
Liang
L
Mu
Xian-liang
XL
Xin
Nan
N
Yang
Ping-ping
PP
Zuo
D000544:Alzheimer Disease / Q000139:chemically induced / Q000235:genetics* / Q000503:physiopathology; D016229:Amyloid beta-Peptides; D000818:Animals; D020869:Gene Expression Profiling; D008297:Male; D018782:Maze Learning / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D009844:Oligosaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D010446:Peptide Fragments; D000459:Phaeophyceae / Q000737:chemistry; D011897:Random Allocation
D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D009844:Oligosaccharides; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35)