[Study of single nucleotide polymorphism of A-FABP gene and its association with fatness traits in chicken].
In this experiment, Beijing-You chicken was used to detect single nucleotide polymorphisms (SNPs) in A-FBAP gene and to study the correlation between its genotype and the trait of fat accumulation. The first exon of the gene was amplified by one pair of primers, and SNPs were detected by the technique of single strand conformation polymorphism (SSCP) and finally confirmed by sequencing. Results of analysis of variance showed that a significant difference existed among abdominal fat percentage, subcutaneous fat thickness and intramuscular fat contents in breast musculature with different genotypes (P<0.01). It implied that A-FABP gene could be a major effector gene or could be linked to gene(s) which significantly affect fat metabolism in chicken.
Luo
Gui-Fen
GF
Institute Animal Science, CAAS , Beijing 100094, China. lgf5156@sina.com
Chen
Ji-Lan
JL
Wen
Jie
J
Zhao
Gui-Ping
GP
Zheng
Mai-Qing
MQ
Sun
Shi-Duo
SD
D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D001823:Body Composition / Q000235:genetics*; D002645:Chickens; D005091:Exons / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005838:Genotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational
D050556:Fatty Acid-Binding Proteins