A simple high performance liquid chromatography assay for monitoring plasma concentrations of tipranavir in HIV infected patients.
A simple HPLC assay to determine plasma concentration of tipranavir is presented. A liquid/liquid extraction of the drugs in ethyl acetate/hexane from 250 microL of plasma is followed by a reversed phase isocratic HPLC assay with UV detection at 205 nm. The imprecision and inaccuracy are lower than 10%, the low limit of quantitation is 0.4 mg/L. Thus, this method can be used for therapeutic drug monitoring of tipranavir in HIV infected patients.
Clinical Pharmacology Department, Hôtel Dieu, Nantes, France. eric.dailly@chu-nantes.fr
E
E
Dailly
V
V
Reliquet
C
C
Victorri-Vigneau
F
F
Raffi
P
P
Jolliet
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015658:HIV Infections / Q000097:blood / Q000188:drug therapy*; D006801:Humans; D011725:Pyridines / Q000097:blood* / Q000627:therapeutic use; D011753:Pyrones / Q000097:blood* / Q000627:therapeutic use; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood* / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides
D011725:Pyridines; D011753:Pyrones; D018894:Reverse Transcriptase Inhibitors; D013449:Sulfonamides; C107201:tipranavir