[Small interfering RNA-mediated MAPK p42 silencing induces apoptosis of HeLa cells].
OBJECTIVE
To observe the effect of small interfering RNA (siRNA)-induced MAPK p42 silencing on the survival of HeLa cells.


METHODS
Two siRNAs targeting at the MAPK p42 gene and one random siRNA were synthesized respectively by Silencer siRNA Construction Kit and transfected into HeLa cells by Lipofectamin 2000. The expression of p42(MAPK) in the transfected HeLa cells was analyzed by Western blotting and immunohistochemistry, and the morphology of cells were observed with electron microscope. TUNEL assay and Annexin V/PI staining were employed for detecting the cell apoptosis.


RESULTS
The expression of p42(MAPK) in the HeLa cells was remarkably suppressed after transfection with the two siRNAs, reduced by about 2.5 and 3.2 folds respectively in comparison with the negative control. Chromatin margination in the cell nuclei were observed in the transfected cells, and TUNEL assay and Annexin V/PI staining further confirmed the occurrence of cell apoptosis.


CONCLUSIONS
In vitro MAPK p42 siRNA-1 and siRNA-2 transfection can specifically silence the gene expression and induce apoptosis of HeLa cells.
Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Xi'an 710061, China.
Chen
C
Huang
Li-ying
LY
Liu
Tu-sheng
TS
Song
Lei
L
Ni
Li-ping
LP
Song
Lü-sheng
LS
Si
D017209:Apoptosis / Q000502:physiology*; D020868:Gene Silencing / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000096:biosynthesis / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D034741:RNA, Small Interfering; D019950:Mitogen-Activated Protein Kinase 1