Coagulase gene typing of Staphylococcus aureus isolated from cows with mastitis in southeastern Brazil.
A typing procedure based on polymorphism of the coagulase gene (coa) was used to discriminate Staphylococcus aureus isolated from Minas Gerais dairy cows with mastitis. Amplification of the gene from the 64 S. aureus isolates produced 27 different polymerase chain reaction (PCR) products; 60 isolates showed only 1 amplicon, and 4 showed 2 amplicons. The isolates were grouped into 49 types by analyzing the restriction fragment length polymorphism (RFLP) of the coa gene; the 10 most common types accounted for 39% of the isolates. The results demonstrate that many variants of the coa gene are present in the studied region, although only a few predominate.
Rodrigues da Silva
Elizabete
E
Laboratory of Mastitis Control, Veterinary School - Federal University of Minas Gerais/UFMG, Belo Horizonte, Minas Gerais, Brazil. beteers@hotmail.com
da Silva
Nivaldo
N
D000818:Animals; D015373:Bacterial Typing Techniques; D001938:Brazil; D002417:Cattle; D003030:Coagulase / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D005784:Gene Amplification; D005838:Genotype; D008414:Mastitis, Bovine / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification / Q000201:enzymology / Q000235:genetics*
D003030:Coagulase; D004269:DNA, Bacterial