Construction of hybrib Autographa californica nuclear polyhedrosis bacmid by modification of p143 helicase.
We developed a new hybrid nuclear polyhedrosis virus (NPV) bacmid capable of infecting Spodoptera frugiperda, Tricoplusia ni, and Bombyx mori, and B. mori cell lines for producing hybrid recombinant baculovirus that can carry a gene of interest and express it in a broad range of hosts. A GFP(uv)-beta1,3-N-acetylglucosaminyltransferase 2 fusion gene was expressed successfully in silkworm larvae using this hybrid bacmid. The hybrid NPV bacmid provides an altogether simple and realistically feasible method for large-scale applications using silkworm larvae. It can be easily managed in E. coli, which has no biohazard safety concerns, in addition to the baculovirus-based expression system.
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan.
Vipin Kumar
VK
Deo
Masato
M
Hiyoshi
Enoch Y
EY
Park
D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000378:metabolism; D002460:Cell Line; D004265:DNA Helicases / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D006824:Hybridization, Genetic; D007313:Insecta; D007814:Larva / Q000378:metabolism; D017351:N-Acetylglucosaminyltransferases / Q000096:biosynthesis / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis; D014170:Transformation, Genetic
D011994:Recombinant Proteins; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; D004265:DNA Helicases