The spore photoproduct lyase repairs the 5S- and not the 5R-configured spore photoproduct DNA lesion.
The spore photoproduct lyase is a Fe-S/AdoMet DNA repair enzyme, which directly repairs spore lesions, induced by UV irradiation of spores, using an unknown radical mechanism. The air sensitive radical SAM enzyme was for the first time challenged with synthetically pure substrates. It was found that the enzyme recognizes a synthetic 5S-configured spore lesion without the central phosphodiester bond. The 5R-configured lesion is in contrast to current belief not a substrate.
Friedel
Marcus G
MG
Department of Chemistry and Biochemistry, LMU Munich, Butenandtstr. 5-13, D-81377, Munich, Germany.
Berteau
Olivier
O
Pieck
J Carsten
JC
Atta
Mohamed
M
Ollagnier-de-Choudens
Sandrine
S
Fontecave
Marc
M
Carell
Thomas
T
D001412:Bacillus subtilis / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair; D010755:Organophosphates / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D010755:Organophosphates; D011506:Proteins; C088014:spore photoproduct lyase