Gene expression analysis using filter cDNA microarrays.
The analysis of gene expression patterns by filter-based complementary (c)DNA microarray remains an important technique in the molecular biology laboratory, despite the development of large-scale cDNA microarray analysis (see Chapter 27). This chapter provides an overview of the methods necessary to carry out the production of membrane-based cDNA arrays and the subsequent synthesis and hybridization of radiolabeled cDNA probes.
Simpson
Peter
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The Breakthrough Toby Robins Breast Research Centre at the Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Jones
Chris
C
Mackay
Alan
A
Lakhani
Sunil R
SR
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods