Preparation and evaluation of unimolecular pentavalent and hexavalent antigenic constructs targeting prostate and breast cancer: a synthetic route to anticancer vaccine candidates.
Several novel, fully synthetic, carbohydrate-based antitumor vaccines have been assembled. Each construct consists of multiple cancer-related antigens displayed on a single polypeptide backbone. Recent advances in synthetic methodology have allowed for the incorporation of a complex oligosaccharide terminating in a sialic acid residue (i.e., GM2) as one of the carbohydrate antigens. Details of the vaccine synthesis as well as the results of preliminary immunological investigations are described herein.
Laboratory of Tumor Vaccinology, Clinical Immunology Service, Department of Medicine, the Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, New York, New York 10021, USA.
Govind
G
Ragupathi
Fusataka
F
Koide
Philip O
PO
Livingston
Young Shin
YS
Cho
Atsushi
A
Endo
Qian
Q
Wan
Maria K
MK
Spassova
Stacy J
SJ
Keding
Jennifer
J
Allen
Ouathek
O
Ouerfelli
Rebecca M
RM
Wilson
Samuel J
SJ
Danishefsky
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology* / Q000628:therapy; D019496:Cancer Vaccines / Q000138:chemical synthesis* / Q000276:immunology; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D005260:Female; D005678:G(M2) Ganglioside / Q000737:chemistry / Q000276:immunology; D006027:Glycosides / Q000737:chemistry; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011471:Prostatic Neoplasms / Q000276:immunology* / Q000628:therapy; D012794:Sialic Acids / Q000737:chemistry
D000596:Amino Acids; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D006027:Glycosides; D009844:Oligosaccharides; D010455:Peptides; D012794:Sialic Acids; D005678:G(M2) Ganglioside; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin