Analysis of potential binding of the recombinant Rab9 effector p40 to phosphoinositide-enriched synthetic liposomes.
The transport factor p40 is thought to assist Rab9 in mediating late-endosome-to-TGN vesicular transport. p40 was recently identified as an associated protein of the PtdIns 5-P/PtdIns 3,5-P2-producing kinase PIKFyve. Moreover, p40 recovery in membrane fractions appeared totally dependent on the presence of an intact enzymatic activity of PIKFyve, implying a mechanism of p40 membrane association dependent on membrane PtdIns 5-P and/or PtdIns 3,5-P2. Here we have evaluated plausible interaction of recombinant p40 with the PIKFyve products PtdIns 5-P and PtdIns 3,5-P2 by a synthetic-liposome binding assay.
Diego
D
Sbrissa
Ognian C
OC
Ikonomov
Assia
A
Shisheva
D001483:Base Sequence; D002460:Cell Line; D002846:Chromatography, Affinity; D017931:DNA Primers; D006801:Humans; D008081:Liposomes; D010716:Phosphatidylinositols / Q000378:metabolism*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
D017931:DNA Primers; D008081:Liposomes; D010716:Phosphatidylinositols; D011993:Recombinant Fusion Proteins; C079856:RAB9A protein, human; D020691:rab GTP-Binding Proteins