Identification of antibodies against HAI-1 and integrin alpha6beta4 as immunohistochemical markers of human villous cytotrophoblast.
Syncytiotrophoblast and invasive extravillous trophoblast arise from a common stem cell, namely villous cytotrophoblast, but have very different characteristics. The study of the differentiation process relies on the availability of suitable markers for these different cell types of developing placenta. In this work, we have produced monoclonal antibodies that are specific to human villous cytotrophoblast. Monoclonal antibody (MAb) MG2 was specific to villous cytotrophoblast across gestation, and recognizes hepatocyte growth factor activator inhibitor type 1. MAb MD10 stained villous cytotrophoblast across gestation and also some endothelial cells, particularly in the second or third trimester. MAb MD10 recognizes human integrin alpha6beta4. As a test for specificity, the novel MAbs were also used for staining of frozen tissue from human colon carcinoma. The results show that the two antibodies can be used as tools to study human villous cytotrophoblasts and also human tumors. The MG2 antibody seems most specific and promising for the study of various aspects of human villous cytotrophoblast.
Hallikas
Outi K
OK
Department of Biological and Environmental Sciences, Division of Genetics, University of Helsinki, Helsinki, Finland.
Aaltonen
Johanna M
JM
von Koskull
Harriet
H
Lindberg
Lars-Axel
LA
Valmu
Leena
L
Kalkkinen
Nisse
N
Wahlström
Torsten
T
Kataoka
Hiroaki
H
Andersson
Leif
L
Lindholm
Dan
D
Schröder
Jim
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000276:immunology* / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D039161:Integrin alpha6beta4 / Q000276:immunology* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D053491:Proteinase Inhibitory Proteins, Secretory; D014327:Trophoblasts / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D039161:Integrin alpha6beta4; D008562:Membrane Glycoproteins; D053491:Proteinase Inhibitory Proteins, Secretory; C109149:SPINT1 protein, human