[Labeling of biocontrol agents ZJY-1 and ZJY-116 gfp gene and its ecological adaptability in cucumber rhizosphere].
The recombined plasmid pRP22-GFP contained with gfp gene and chloramphenicol resistant was successfully introduced into two biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116. After seed inoculation, the survival and colonization of the two strains were studied by periodically retrieving the GFP-tagged strains in the cucumber rhizosphere based on the selective markers. The results showed that both the strains could successfully colonize in the rhizosphere during the whole life of cucumber, and a higher colonization level was observed during anthesis and fruition stages. In pot trials, they could migrate to the nearby non-inoculated spontaneous weed plants, and reestablish in the rhizosphere of plants subsequently grown in the same pot.
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China. xinzhangw@eyou.com
Xin
X
Zhang
Bingxin
B
Zhang
Jingquan
J
Yu
Zhen
Z
Zhang
Weifeng
W
Shen
Zhenyu
Z
Chen
Jiang
J
Shi
D000222:Adaptation, Physiological; D001412:Bacillus subtilis / Q000502:physiology*; D001951:Brevibacterium / Q000502:physiology*; D018553:Cucumis sativus / Q000382:microbiology* / Q000502:physiology; D017753:Ecosystem; D049452:Green Fluorescent Proteins / Q000235:genetics; D018517:Plant Roots / Q000382:microbiology* / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins