Capillary electrophoresis enantioselective separation of vigabatrin enantiomers by precolumn derivatization with dehydroabietylisothiocyante and UV-vis detection.
A simple and reliable capillary electrophoresis (CE) method with UV-vis detection is presented for the enantioselective separation and determination of vigabatrin enantiomers. Dehydroabietylisothiocyante (DHAIC), a novel chiral derivatizing reagent, was used for precolumn derivatization of vigabatrin enantiomers. Optimal separation was obtained with a running buffer consisting of 50 mM Na2HPO4 (pH 9.0), 17 mM sodium dodecyl sulfate (SDS) and 25% acetonitrile. The enantiomeric separation of vigabatrin derivatives was achieved within 25 min, and the resolution was found to be 2.1. Detection was followed by direct UV absorptiometric measurements at 202 nm. A calibration curve ranging from 0.3 to 6.0 microg/ml was shown to be linear, and the limit of detection was 0.15 microg/ml. The developed method has been applied to the determination of vigabatrin enantiomers spiked in human plasma, no interferences were found from endogenous amino acids.
Zhao
Shulin
S
College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China. zhaoshulin001@163.com
Zhang
Rongcan
R
Wang
Hengshan
H
Tang
Lidong
L
Pan
Yinming
Y
D045784:Abietanes / Q000737:chemistry*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017879:Isothiocyanates / Q000737:chemistry*; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013237:Stereoisomerism; D020888:Vigabatrin / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*
D045784:Abietanes; D002021:Buffers; D017879:Isothiocyanates; C511654:dehydroabietylisothiocyante; D012967:Sodium Dodecyl Sulfate; D020888:Vigabatrin