Prolactin secretory rhythm of mated rats induced by a single injection of oxytocin.
Mating or vaginocervical stimulation [copulatory stimulus (CS)] induces two daily surges of the hormone prolactin (PRL) in rats. This unique secretory pattern of PRL surges is characteristic for the first half of pregnancy and is also present in ovariectomized (OVX) rats. Studies have shown that CS additionally provokes an acute release of the hormone oxytocin (OT). In this study, we tested whether a single injection of OT (iv) is sufficient to initiate the PRL secretion pattern of OVX/CS rats. Furthermore, we measured the 24-h profile of dopamine (DA) content in the anterior lobe of the pituitary gland, because DA is the major inhibitory factor of PRL secretion. The results indicated that a single injection of OT induces a PRL secretory rhythm and a DA release pattern similar to that initiated by CS. Immunocytochemical investigation showed that particular OTergic neurons in the hypothalamus express receptors for PRL, as well as for vasoactive intestinal polypeptide, which indicates an involvement in generating the PRL rhythm and entraining it to the ambient photoperiod. On the basis of this study, we suggest that the PRL-DA inhibitory feedback loop between lactotrophs and DAergic neurons plays a crucial role in generating the oscillatory PRL secretion pattern in CS rats. A timing signal, likely provided by the hypothalamic suprachiasmatic nucleus, entrains the autonomous PRL oscillation to a particular time of day. Mathematical modeling was used to illustrate the proposed network function. The experimental results further suggest an additional feedback mechanism in which certain hypothalamic OTergic neurons are influenced by PRL.
Egli
Marcel
M
Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Bertram
Richard
R
Toporikova
Natalia
N
Sellix
Michael T
MT
Blanco
Wilfredo
W
Freeman
Marc E
ME
D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects / Q000502:physiology; D003307:Copulation / Q000502:physiology*; D004298:Dopamine / Q000097:blood / Q000502:physiology; D005246:Feedback; D005260:Female; D007150:Immunohistochemistry; D008954:Models, Biological; D010052:Ovariectomy; D010121:Oxytocin / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism; D011388:Prolactin / Q000097:blood / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011981:Receptors, Prolactin / Q000378:metabolism; D051239:Receptors, Vasoactive Intestinal Peptide, Type II / Q000378:metabolism
D011981:Receptors, Prolactin; D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D010121:Oxytocin; D011388:Prolactin; D004298:Dopamine