Development of a direct species-specific PCR assay for differential diagnosis of Leishmania tropica.
We have developed a polymerase chain reaction assay for differential diagnosis of Leishmania tropica, based on simple amplification of the target region. The assay detects less than 5 protozoan cells, was tested on human samples and an experimentally infected animal, and is appropriate for clinical laboratories in countries where leishmaniasis is endemic.
Jirkù
Milan
M
Institute of Parasitology, Czech Academy of Sciences, 37005 Ceské Budejovice, Czech Republic.
Zemanová
Eva
E
Al-Jawabreh
Amer
A
Schönian
Gabriele
G
Lukes
Julius
J
D000328:Adult; D000818:Animals; D002648:Child; D002675:Child, Preschool; D006224:Cricetinae; D016054:DNA, Protozoan / Q000032:analysis; D003937:Diagnosis, Differential; D006801:Humans; D007895:Leishmania tropica / Q000235:genetics* / Q000302:isolation & purification; D007896:Leishmaniasis / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D008647:Mesocricetus; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity
D016054:DNA, Protozoan