Relationships between the expressions of CDX1 and CDX2 mRNA and clinicopathologic features in colorectal cancers.
BACKGROUND
CDX1 and CDX2 are members of the caudal-type homeobox gene family and control the proliferation and differentiation of intestinal mucosal cells. Their expressions are commonly reduced in colorectal cancer, but reports about the relationships between their expressions and clinicopathologic features are rare. The aim of this study was to examine the expressions of CDX1 and CDX2 mRNAs in colorectal cancers and to assess the relationships between their expressions and clinicopathologic features.


METHODS
CDX1 and CDX2 mRNA expressions were analyzed by real-time polymerase chain reaction in 48 colorectal cancers and in adjacent non-tumorous normal mucosal tissue.


RESULTS
CDX1 and CDX2 mRNA expressions were significantly reduced in colorectal cancer tissues versus normal mucosal tissues (p=0.001, p=0.042, respectively). As compared with paired normal mucosal tissues, colorectal tissues showed reduced CDX1 mRNA expression in 64.6% (31/48) and reduced CDX2 mRNA expression in 66.7% (32/48) of cases. A statistically significant positive correlation was found between the expressions of CDX1 mRNA and CDX2 mRNA in colorectal cancer (r=0.543, p<0.001). However, the expressions of CDX1 and CDX2 mRNAs were not related to age, sex, cancer location, differentiation, lymphatic or vascular invasion, lymph node metastasis, stage or serum carcinoembryonic antigen level.


CONCLUSIONS
CDX1 and CDX2 mRNA expressions were found to be significantly reduced in colorectal cancers but these expressional changes were not found to be related to clinicopathologic features.
Kim
Gwang Ha
GH
Department of Internal Medicine, Pusan National University College of Medicine, Busan, Korea.
Lee
Dong Hyun
DH
Kim
Hyung Wook
HW
Cheong
Jong Yun
JY
Seo
Soo Boon
SB
Heo
Jeong
J
Kang
Dae Hwan
DH
Song
Geun Am
GA
Cho
Mong
M
Yang
Ung Suk
US
Park
Do Youn
DY
Yoo
Mi Ae
MA
D000071616:CDX2 Transcription Factor; D015179:Colorectal Neoplasms / Q000378:metabolism*; D005260:Female; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*
C491273:CDX1 protein, human; D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; D018398:Homeodomain Proteins; D012333:RNA, Messenger