An in vitro study of hematopoietic progenitor cells from peripheral blood of rabbits.
The purpose of this study was to isolate and cultivate a subpopulation of pluripotent stem cells (PSCs) from the peripheral blood of rabbits, which are frequently used in veterinary research as an animal model. Pluripotent stem cells, as described in human beings, are fibroblast-like cells that exhibit a CD34 marker, specific from other hematopoietic stem cells. Commonly used human commercial media has been researched for culturing rabbit PSCs. These findings allow us to contemplate the direct application of this simple and standardized methodology in several areas of study, such as of the pharmacological effect of many drugs on hematopoietic cells, veterinary practice, and even the study of new strategies in cellular therapy for some human diseases.
Department of Animal Pathology, Veterinary Faculty of Zaragoza, C/ Miguel Servet, 177 CP 50013, Zaragoza, Spain. pablog@unizar.es
Pablo
P
Gómez-Ochoa
Francisco Javier
FJ
Miana-Mena
María Jesús
MJ
Muñoz
Encarna
E
Cativiela
Francisco
F
Gómez
D000818:Animals; D018952:Antigens, CD34; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D008297:Male; D011817:Rabbits / Q000097:blood*; D012106:Research
D018952:Antigens, CD34; D003470:Culture Media