High-performance liquid chromatographic enantioseparations on capillary columns containing monolithic silica modified with amylose tris(3,5-dimethylphenylcarbamate).
Monolithic capillary columns containing native silica were modified by in situ coating with amylose tris(3,5-dimethylphenylcarbamate) and applied for enantioseparations in capillary liquid chromatography. Capillary columns were examined for 10 standard racemic compounds in order to compare the performance of monolithic silica columns with the common, 4.6mm I.D. high-performance liquid chromatographic columns packed with particulate silica. The effects of polysaccharide coating and of the linear velocity of the mobile phase on peak performance were studied. Enantioseparations with an analysis time below 1min were achieved for some chiral analytes.
Molecular Recognition and Separation Science Laboratory, School of Chemistry, Tbilisi State University, Chavchavadze Ave 1, 028 Tbilisi, Georgia.
Bezhan
B
Chankvetadze
Chiyo
C
Yamamoto
Masami
M
Kamigaito
Nobuo
N
Tanaka
Kazuki
K
Nakanishi
Yoshio
Y
Okamoto
D000688:Amylose / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D048448:Phenylcarbamates / Q000737:chemistry*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
D048448:Phenylcarbamates; C080033:Chiralpak AD; D012822:Silicon Dioxide; D000688:Amylose; D010626:Phenylethyl Alcohol