Effects of ingestion of difructose anhydride III (DFA III) and the DFA III-assimilating bacterium Ruminococcus productus on rat intestine.
We have isolated a difructose anhydride III (DFA III)-assimilating bacterium, Ruminococcus productus AHU1760, from human. After an acclimation period of 1 week, male Sprague-Dawley rats (5 weeks old) were divided into four groups (control diet, R. productus diet, DFA III diet, and R. productus + DFA III diet; n = 8) and fed the assigned test diets for 2 weeks. The viable count of administered R. productus was 4.9 x 10(7) CFU/d in R. productus-fed rats and 4.7 x 10(7) CFU/d in R. productus + DFA III-fed rats. Survival in cecal content of this strain was confirmed by randomly amplified polymorphic DNA. The ratio of secondary bile acids in feces in R. productus + DFA III-fed rats decreased the same as that in rats fed only DFA III. The viable count of lactobacilli and bifidobacteria, known as beneficial bacteria, increased more in R. productus + DFA III-fed rats than in control or R. productus-fed rats. A combination of R. productus and DFA III might improve the balance of intestinal microbiota to a healthier condition.
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Japan.
Kimiko
K
Minamida
Midori
M
Ohashi
Hiroshi
H
Hara
Kozo
K
Asano
Fusao
F
Tomita
D000818:Animals; D001647:Bile Acids and Salts / Q000737:chemistry / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002432:Cecum / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology*; D002457:Cell Extracts / Q000008:administration & dosage / Q000494:pharmacology; D004187:Disaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects; D005243:Feces / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D045857:Ruminococcus / Q000235:genetics / Q000378:metabolism*; D015996:Survival Rate
D001647:Bile Acids and Salts; D002457:Cell Extracts; D004187:Disaccharides; C087758:difructose anhydride III