DNA-protein cross-linking: model systems for pyrimidine-aromatic amino acid cross-linking.
[reaction: see text] We have synthesized simple model systems to explore the possibility of photo-cross-linking between the pyrimidine bases and the side chains of the aromatic amino acids. Thymine/phenylalanine and thymine/tyrosine models gave cross-links, and thymine/tryptophan models gave complex mixtures; the cytosine/phenylalanine model was unreactive. The quantum yields for the model cross-linking reactions were 18-46 times smaller than those for thymine dimer formation. Biphotonic excitation contributes little to the yield of these reactions.
Sun
Guangxing
G
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
Fecko
Christopher J
CJ
Nicewonger
Robert B
RB
Webb
Watt W
WW
Begley
Tadhg P
TP
D024322:Amino Acids, Aromatic / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D008954:Models, Biological; D011506:Proteins / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry*
D024322:Amino Acids, Aromatic; D003432:Cross-Linking Reagents; D011506:Proteins; D011743:Pyrimidines; D004247:DNA; C030986:pyrimidine