TP53 mutations in salivary gland neoplasms.
Many studies have demonstrated that loss of TP53 gene function has an important role in the genesis of many neoplasms, including salivary gland neoplasms. The purpose of this study was to examine the mutation profile of the TP53 gene in salivary gland neoplasms. Genomic DNA was extracted from paraffin-embedded tissues of pleomorphic adenoma, carcinoma in pleomorphic adenoma, mucoepidermoid carcinoma, adenoid cystic carcinoma and polymorphous low grade adenocarcinoma. Exons 5 to 8 of the TP53 gene were amplified by polymerase chain reaction (PCR) to perform single-stranded conformational polymorphism (SSCP) analysis. Band shifting was observed in exons 5, 6 and 8 in 9 out of 18 neoplasms. The results of this study suggest that mutations in TP53 gene are related to salivary gland neoplasms pathogenesis and that exons 5 and 8 are most frequently involved.
Department of Oral Pathology, School of Dentistry, University of São Paulo (FOUSP), Brazil.
Luciana Fasanella
LF
Matizonkas-Antonio
Ricardo Alves
RA
de Mesquita
Suzana C Orsini Machado
SC
de Souza
Fabio Daumas
FD
Nunes
D000230:Adenocarcinoma / Q000235:genetics; D008949:Adenoma, Pleomorphic / Q000235:genetics; D002277:Carcinoma / Q000235:genetics; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics; D018277:Carcinoma, Mucoepidermoid / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005091:Exons / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics*; D016678:Genome / Q000235:genetics; D006801:Humans; D009154:Mutation / Q000235:genetics*; D009378:Neoplasms, Multiple Primary / Q000235:genetics; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012468:Salivary Gland Neoplasms / Q000235:genetics*; D012470:Salivary Glands, Minor / Q000473:pathology
D004273:DNA, Neoplasm