Type I and type III collagen-platelet interaction: inhibition by type specific receptor peptides.
We have previously cloned and characterized a platelet receptor for type III collagen (47 kDa) from a human bone marrow cDNA phage library and defined two active peptides. We also cloned and characterized a platelet receptor for type I collagen (65 kDa) and defined an active peptide. Our objective was to study whether there is type specificity of these active peptides. We have engineered a mutant receptor clone by replacing one of the two active peptides of the platelet receptor for type III collagen with the active peptide of the platelet receptor for type I collagen. The replacement of an active peptide at the amino terminal end (rMIII) of the platelet receptor for type III collagen with the type I collagen active peptide was done without altering the hydrophilicity of the protein. This purified recombinant protein reacts with polyclonal anti-47-kDa and anti-65-kDa active peptide antibodies. The purified recombinant protein inhibits both types I and III collagen-induced platelet aggregation. This rMIII also inhibits the adhesion of washed platelets to rabbit aortic segments (natural matrix) in a dose-dependent manner. The chemically synthesized hybrid peptide of each active peptide of platelet type I and type III collagen receptors inhibits types I and III collagen-induced platelet aggregation in a dose-dependent manner. These results suggest that there is a type specific reactive site on platelets for type I and type III collagens.
Veterans Administration Medical Center, University of Tennessee Health Science Center, 1030 Jefferson Avenue, Memphis, TN 38104, USA.
Jiaqian
J
Zhu
Flecia
F
Cole
Virginia
V
Woo-Rasberry
Xiao Rong
XR
Fang
Thomas M
TM
Chiang
D000818:Animals; D001011:Aorta / Q000378:metabolism; D001435:Bacteriophages / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D002448:Cell Adhesion; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D015723:Gene Library; D006801:Humans; D009154:Mutation; D010455:Peptides / Q000737:chemistry; D010974:Platelet Aggregation; D011485:Protein Binding; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry
D024042:Collagen Type I; D024061:Collagen Type III; D010455:Peptides; D011994:Recombinant Proteins