Nonviral gene delivery from nonwoven fibrous scaffolds fabricated by interfacial complexation of polyelectrolytes.
We investigated a novel nonwoven fibrous scaffold as a vehicle for delivery of DNA. Fibers were formed by polyelectrolyte complexation of water-soluble chitin and alginate, and PEI-DNA nanoparticles were encapsulated during the fiber drawing process. Nanoparticles released from the fibers over time retained their bioactivity and successfully transfected cells seeded on the scaffold in a sustained manner. Transgene expression in HEK293 cells and human dermal fibroblasts seeded on the transfecting scaffolds was significant even after 2 weeks of culture compared to 3-day expression in two-dimensional controls. Fibroblasts seeded on scaffolds containing DNA encoding basic fibroblast growth factor (bFGF) demonstrated prolonged secretion of bFGF at levels significantly higher than baseline. This work establishes the potential of this fibrous scaffold as a matrix capable of delivering genes to direct and support cellular development in tissue engineering.
Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Shawn H
SH
Lim
I-Chien
IC
Liao
Kam W
KW
Leong
D000464:Alginates / Q000737:chemistry; D002470:Cell Survival; D002478:Cells, Cultured; D002686:Chitin / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004573:Electrolytes; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D018014:Gene Transfer Techniques; D020723:Glucuronic Acid / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D036103:Nanotechnology / Q000379:methods*; D009940:Organoids / Q000166:cytology*; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry; D023822:Tissue Engineering; D014162:Transfection; D014780:Viruses / Q000235:genetics
D000464:Alginates; D004573:Electrolytes; D006603:Hexuronic Acids; D016222:Fibroblast Growth Factor 2; D002686:Chitin; D049452:Green Fluorescent Proteins; D020723:Glucuronic Acid; D011094:Polyethyleneimine; D004247:DNA