Pleiotropic phenotype in Entamoeba histolytica overexpressing DNA methyltransferase (Ehmeth).
The protozoan parasite Entamoeba histolytica expresses a cytosine-5 DNA methyltransferase (Ehmeth) that belongs to the Dnmt2 proteins family. The biological function of members of the Dnmt2 family is unknown. Constitutive overexpression of Ehmeth resulted in a pleiotropic phenotype that includes accumulation of multinucleated cells, upregulation of Heat shock protein 70 (HSP70) expression and resistance to oxidative stress. This pleiotropic phenotype suggests that Ehmeth plays an important role in the control of key cellular processes in the parasite.
Department of Molecular Microbiology, The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, P.O.B. 9649, 31096 Haifa, Israel.
Ohad
O
Fisher
Rama
R
Siman-Tov
Serge
S
Ankri
D000595:Amino Acid Sequence; D000818:Animals; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D016054:DNA, Protozoan / Q000378:metabolism; D004748:Entamoeba histolytica / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010641:Phenotype
D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D004248:DNA (Cytosine-5-)-Methyltransferases