Collision-induced dissociation pathways of yeast sphingolipids and their molecular profiling in total lipid extracts: a study by quadrupole TOF and linear ion trap-orbitrap mass spectrometry.
The yeast Saccharomyces cerevisiae synthesizes three classes of sphingolipids: inositolphosphoceramides (IPCs), mannosyl-inositolphosphoceramides (MIPCs), and mannosyl-diinositolphosphoceramides (M(IP)2C). Tandem mass spectrometry of their molecular anions on a hybrid quadrupole time-of-flight (QqTOF) instrument produced fragments of inositol-containing head groups, which were specific for each lipid class. MS(n) analysis performed on a hybrid linear ion trap-orbitrap (LTQ Orbitrap) mass spectrometer with better than 3 ppm mass accuracy identified fragment ions specific for the amide-linked fatty acid and the long chain base moieties in individual molecular species. By selecting m/z of class-specific fragment ions for multiple precursor ion scanning, we profiled yeast sphingolipids in total lipid extracts on a QqTOF mass spectrometer. Thus, a combination of QqTOF and LTQ Orbitrap mass spectrometry lends itself to rapid, comprehensive and structure-specific profiling of the molecular composition of sphingolipids and glycerophospholipids in important model organisms, such as fungi and plants.
Ejsing
Christer S
CS
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Moehring
Thomas
T
Bahr
Ute
U
Duchoslav
Eva
E
Karas
Michael
M
Simons
Kai
K
Shevchenko
Andrej
A
D020404:Glycerophospholipids / Q000032:analysis / Q000737:chemistry; D006028:Glycosphingolipids / Q000032:analysis / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013107:Sphingolipids / Q000032:analysis* / Q000737:chemistry; D013110:Sphingosine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry
D020404:Glycerophospholipids; D006028:Glycosphingolipids; D013107:Sphingolipids; C025524:inositolphosphoceramides; C479822:mannosylinositol phosphoylceramide; D013110:Sphingosine; C005682:safingol