Polymer-based monolithic microcolumns for hydrophobic interaction chromatography of proteins.
Monolithic capillary columns for hydrophobic interaction chromatography (HIC) have been prepared by thermally initiated, single-step in situ polymerization of mixtures of monovinyl monomers including butyl methacrylate and/or 2-hydroxyethyl methacrylate, with a divinyl crosslinker glycerol dimethacrylate or 1,4-butanediol dimethacrylate using two different porogen systems. Two porogenic solvent mixtures were used; one "hydrophilic", consisting of water, butanediol, and propanol, and one "hydrophobic," comprising dodecanol and cyclohexanol. The porous structures of the monoliths were characterized and their performance was demonstrated with a separation of a mixture of myoglobin, ribonuclease A, and lysozyme under conditions typical of HIC.
Hemström
Petrus
P
Department of Chemistry, Umeå University, Umeå, Sweden.
Nordborg
Anna
A
Irgum
Knut
K
Svec
Frantisek
F
Fréchet
Jean M
JM
D000433:1-Propanol / Q000737:chemistry; D002072:Butylene Glycols / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D003511:Cyclohexanols / Q000737:chemistry; D007851:Dodecanol / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D014867:Water / Q000737:chemistry
D002072:Butylene Glycols; D003511:Cyclohexanols; D008689:Methacrylates; D011506:Proteins; D014867:Water; D007851:Dodecanol; D000433:1-Propanol