Overproduction of PGE2 in peripheral blood monocytes of gastrointestinal cancer patients with mucins in their bloodstream.
When monocytes from healthy donors were cultured in the presence of sera from patients with gastrointestinal cancer, PGE2 production from the monocytes was elevated. Serum proteins were fractionated on Sepharose 4B and the inducing activity was found in the excluded fractions. By excluding some mucins from the serum, the inducing activity was reduced effectively. The activity was also reduced by adding binding inhibitors to the scavenger receptor. These results suggest that peripheral blood monocytes in epithelial cancer patients may be continuously stimulated by mucins in the bloodstream through the scavenger receptor, resulting in overproduction of PGE2.
Yokoigawa
Norio
N
Department of Surgery, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8507, Japan.
Takeuchi
Noriko
N
Toda
Munetoyo
M
Inoue
Mizue
M
Kaibori
Masaki
M
Yanagida
Hidesuke
H
Inaba
Takaaki
T
Tanaka
Hironori
H
Ogura
Tokuhiro
T
Takada
Hideho
H
Okumura
Tadayoshi
T
Kwon
A-Hon
AH
Kamiyama
Yasuo
Y
Nakada
Hiroshi
H
D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000494:pharmacology; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000235:genetics; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D017308:Etodolac / Q000494:pharmacology; D005770:Gastrointestinal Neoplasms / Q000097:blood* / Q000201:enzymology / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009077:Mucins / Q000097:blood / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051116:Receptors, Scavenger / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013433:Sulfoglycosphingolipids / Q000494:pharmacology
D001798:Blood Proteins; D016861:Cyclooxygenase Inhibitors; D009077:Mucins; D009842:Oligopeptides; D012333:RNA, Messenger; D051116:Receptors, Scavenger; D013433:Sulfoglycosphingolipids; D017308:Etodolac; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D007213:Indomethacin