[Fenotypical characteristic of coagulase negative staphylococci isolated from patients hospitalized on burn ward and surgical ward].
The aim of the study was to analysis of the adhesion factors of coagulase-negative staphylococci (CNS) to intravenous catheter: ability to adhesion, slime production, hydrophobicity of surface of cells and susceptibility to antibiotics. The researches heve been done on CNS strains isolated from cardiosurgical patients. Slime production ability was evaluated using plate method according to Christensen and Congo Red Agar method. Adherence of bacterial strains to intravenous catheter (polytetrafluoroethylene) in vitro was determined using the method of Richards. Hydrophobicity of surface of cells was determined on the basis of agregattion in (NH4)2SO4 Susceptibility to antibiotics was determined using the disc-diffusion method. Out of the analyzed strains 19% were labeled as slime producing in the plate method and 14% in the Congo red Agar method. 43% of analyzed strains were found to have TTC reduction of 3+, 19% of 2+ and 28% of 1+. Among these 10% of the assesed strains did not reduce TTC.
Katedra i Zakład Mikrobiologii Akademii Medycznej we Wrocławiu. marzbp@mbio.am.wroc.pl
Marzenna
M
Bartoszewicz
Joanna
J
Nowicka
Anna
A
Rygiel
Jacek
J
Karoń
D001422:Bacterial Adhesion; D002056:Burns / Q000382:microbiology*; D002408:Catheters, Indwelling / Q000382:microbiology; D003030:Coagulase / Q000378:metabolism; D004351:Drug Resistance; D006801:Humans; D010641:Phenotype; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000502:physiology*; D013499:Surface Properties; D013530:Surgical Wound Infection / Q000382:microbiology*
D003030:Coagulase