Levels of G-protein alpha q/11 subunits and of phospholipase C-beta(1-4), -gamma, and -delta1 isoforms in postmortem human brain caudate and cortical membranes: potential functional implications.
The levels of expression of G-protein alpha(q/11) (Galpha(q/11)) subunits and PLC-beta(1-4), -gamma, and -delta(1) isoforms were quantified by Western blot analysis in order to establish their contribution to the patterns of PLC functioning reported here. Quantitative measurements of the levels of Galpha(q/11) subunits in each region were obtained by comparison with known amounts of Escherichia coli expressed recombinant Galpha(q) subunits. Quantitative analysis indicated that Galpha(q/11) subunits are abundant polypeptides in human brain, with values ranging from about 1200 ng/mg in cerebral cortex to close to 900 ng/mg of membrane protein in caudate. In cerebral cortical membranes, the PLC-beta(1) isoform was more abundant than in caudate membranes. The highest levels of PLC-beta(2) expression were detected in caudate membranes. PLC-beta(3) was little expressed, and there were no significant differences in the relative values between both brain regions. Finally, the levels of the PLC-beta(4) isoform were significantly lower in caudate than in cortical membranes. It is concluded that although most of these data represent relative, not absolute, measures of protein levels within these regions, they contribute nonetheless to the significant differences observed in signaling capacities through the PLC system in both human brain regions.
Department of Pharmacology, Faculty of Pharmacy, University of the Basque Country, Spain.
Maider
M
López de Jesús
Amaia
A
Zalduegui
Iñigo
I
Ruiz de Azúa
Luis F
LF
Callado
J Javier
JJ
Meana
Joan
J
Sallés
D000328:Adult; D002421:Caudate Nucleus / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D005260:Female; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D018721:Muscarinic Agonists / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D054799:Phospholipase C beta; D054803:Phospholipase C delta; D051966:Phospholipase C gamma / Q000378:metabolism; D011180:Postmortem Changes; D021122:Protein Subunits / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects; D013347:Subcellular Fractions; D010738:Type C Phospholipases / Q000378:metabolism*
D007527:Isoenzymes; D018721:Muscarinic Agonists; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D021122:Protein Subunits; D043562:Receptors, G-Protein-Coupled; D011976:Receptors, Muscarinic; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D010738:Type C Phospholipases; C518024:PLCD1 protein, human; D054799:Phospholipase C beta; D054803:Phospholipase C delta; D051966:Phospholipase C gamma; D043802:GTP-Binding Protein alpha Subunits, Gq-G11