Blood pressure-depressing activity of a peptide derived from silkworm fibroin in spontaneously hypertensive rats.
Peptides showing inhibitory activity against the angiotensin I-converting enzyme (ACE) were investigated from the fibroin fraction of discarded silk fabric. Fibroin, which was hydrolyzed with alcalase after partial hydrolysis with hot aqueous 40% CaCl(2), released two major active peptides showing ACE-inhibitory activity. The two peptides were identified as glycyl-valyl-glycyl-tyrosine (GVGY) and glycyl-valyl-glycyl-alanyl-glycyl-tyrosine (GVGAGY) by analyses with a protein sequencer and LC/MS/MS. GVGY, whose ACE-inhibitory activity has not previously been reported, showed a blood pressure-depressing effect on spontaneously hypertensive rat (SHR).
Igarashi
Kiharu
K
Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University, Japan. igarashi@tds1.yamagata-u.ac.jp
Yoshioka
Kosuke
K
Mizutani
Kenji
K
Miyakoshi
Masazumi
M
Murakami
Toshiyuki
T
Akizawa
Toshifumi
T
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D005349:Fibroins / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR
D010446:Peptide Fragments; C482288:fibroin, silkworm; D005349:Fibroins