Hepatitis E virus genotyping based on full-length genome and partial genomic regions.
Some genomic regions for hepatitis E virus (HEV) genotyping have been reported to correlate well with the results from the phylogenetic analyses on the basis of the complete genome. However, few studies have systemically investigated the genomic regions for HEV genotyping using a combined phylogenetic and statistical approach. A consensus region for HEV genotyping has not been determined. In this study the nucleotide identities and genetic distances of 24 partial genomic regions and the complete genome sequences of 37 HEV strains were compared statistically. It was demonstrated with both one-way ANOVA and two-way ANOVA that only one genomic region in RNA-dependent RNA polymerase domain (4254-4560nt) for which there were no significant differences when compared with the full-length genome (P>0.05). The same four genotypes were identified by phylogenetic analysis based on this statistically predicted region identified as for the complete genome. RT-PCR amplification of HEV strains from all four genotypes confirmed conservation of the flanking primer sites of this region. Serum samples from 20 patients with a clinical diagnosis of hepatitis E were further analyzed by PCR using the same primers, 13 were positive and could be classified into genotype 4. These data strongly suggested that this newly identified region could be used for future HEV genotyping.
Department of Microbiology and Immunology, School of Medicine, Southeast University, 87# Dingjiaqiao Rd., Nanjing, Jiangsu 210009, China. zhailj@iastate.edu
Lijie
L
Zhai
Xing
X
Dai
Jihong
J
Meng
D000704:Analysis of Variance; D017931:DNA Primers; D016679:Genome, Viral; D016751:Hepatitis E / Q000097:blood / Q000821:virology; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers