The spore photoproduct lyase repairs the 5S- and not the 5R-configured spore photoproduct DNA lesion.
The spore photoproduct lyase is a Fe-S/AdoMet DNA repair enzyme, which directly repairs spore lesions, induced by UV irradiation of spores, using an unknown radical mechanism. The air sensitive radical SAM enzyme was for the first time challenged with synthetically pure substrates. It was found that the enzyme recognizes a synthetic 5S-configured spore lesion without the central phosphodiester bond. The 5R-configured lesion is in contrast to current belief not a substrate.
Department of Chemistry and Biochemistry, LMU Munich, Butenandtstr. 5-13, D-81377, Munich, Germany.
Marcus G
MG
Friedel
Olivier
O
Berteau
J Carsten
JC
Pieck
Mohamed
M
Atta
Sandrine
S
Ollagnier-de-Choudens
Marc
M
Fontecave
Thomas
T
Carell
D001412:Bacillus subtilis / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair; D010755:Organophosphates / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D010755:Organophosphates; D011506:Proteins; C088014:spore photoproduct lyase