Universal Collection Medium (UCM) is as suitable as the Standard Transport Medium (STM) for Hybrid Capture II (HC-2) assay.
BACKGROUND
Collection of cervical-vaginal material in liquid media enables simultaneous evaluation of both oncologic cytology and molecular tests for the detection of Human papillomavirus (HPV), Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG). Universal Collection Medium (UCM) has been developed to fulfill this objective.


OBJECTIVE
To compare Hybrid Capture II (HC-2) to diagnose HPV, NG and CT in specimens collected in UCM and in the current Digene Standard Transport Medium (STM).


METHODS
The study was cross-sectional. Three collections of endocervical and ectocervical material were performed in each of 893 women referred for colposcopy in the following order: (1) to prepare a conventional Pap smear slide using the accompanying brush of the STM kit and with Ayre spatula; (2) for HC-2 test and liquid-based cytology using a 1 ml UCM vial as transport medium; material was collected with another similar brush; (3) for HC-2 test using a 1 ml STM vial as transport medium; material was collected with the same brush that we used in the procedure no. (1) (conventional Pap smear). HC-2 results from samples taken from STM and UCM media were compared by using simple linear regression analysis and Kappa statistic.


CONCLUSIONS
HC-2 results from the two media were highly correlated: high-risk HPV (kappa=0.92; r(2)=0.92), low-risk HPV (kappa=0.85; r(2)=0.86) and NG/CT (kappa=0.96; r(2)=0.81). Despite being obtained from a second specimen, the UCM HC-2 results were equivalent to those obtained with the standard medium STM and the UCM medium.
Department of Gynecology, Federal University of São Paulo-Escola Paulista de Medicina (UNIFESP-EPM), Rua Borges Lagoa, 1231 CJ 62, CEP 04038-034, São Paulo (SP), Brazil. taha@uol.com.br
Nabiha Saadi Abrahão
NS
Taha
José
J
Focchi
Julisa Chamorro Lascasas
JC
Ribalta
Adauto
A
Castelo
Attila
A
Lorincz
Gerson Botacini
GB
Dôres
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002584:Cervix Uteri / Q000821:virology; D002692:Chlamydia trachomatis / Q000302:isolation & purification; D003127:Colposcopy; D003430:Cross-Sectional Studies; D003470:Culture Media / Q000592:standards*; D015344:DNA Probes, HPV; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D016014:Linear Models; D008163:Luminescent Measurements; D008875:Middle Aged; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization / Q000379:methods*; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000302:isolation & purification; D013048:Specimen Handling / Q000379:methods; D014626:Vaginal Smears / Q000379:methods
D000911:Antibodies, Monoclonal; D003470:Culture Media; D015344:DNA Probes, HPV