Conditional knockout mouse for tissue-specific disruption of the cyclooxygenase-2 (Cox-2) gene.
Cyclooxygenase-2 (Cox-2) modulates many normal functions, and appears to play a role in a wide variety of pathophysiologic conditions. Cox-2 gene expression is induced in many different cell types, in response to many distinct stimuli. We generated a conditional knockout mouse in which critical exons of the Cox-2 gene are flanked with loxP sites. Cox-2(flox/flox) mice appear normal and are fertile. Recombination at the loxP sites, loss of Cox-2 protein expression, and prevention of induced PGE2 accumulation are observed in Cox-2(flox/flox) mouse embryo fibroblasts following infection with an adenovirus expressing CRE recombinase. In vivo recombination at the Cox-2(flox) allele was demonstrated in the liver of Cox-2(flox/flox) mice following intravenous injection of adenovirus expressing CRE recombinase. Spatially and temporally restricted elimination of the Cox-2 gene in Cox-2(flox/flox) conditional knockout mice should provide a valuable tool to analyze the cell type-specific role of Cox-2 in many disease models.
Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA.
Tomo-O
TO
Ishikawa
Harvey R
HR
Herschman
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000235:genetics*; D004622:Embryo, Mammalian / Q000166:cytology / Q000201:enzymology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D018390:Gene Targeting; D006579:Heterozygote; D006720:Homozygote; D019426:Integrases / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D016133:Polymerase Chain Reaction; D011453:Prostaglandins / Q000378:metabolism; D011995:Recombination, Genetic; D017384:Sequence Deletion; D014162:Transfection
D011453:Prostaglandins; D051546:Cyclooxygenase 2; C045073:Cre recombinase; D019426:Integrases