Cytokine regulation in SARS coronavirus infection compared to other respiratory virus infections.
The pathogenesis of severe acute respiratory syndrome (SARS) is poorly understood and cytokine dysregulation has been suggested as one relevant mechanism to be explored. We compared the cytokine profile in Caco2 cells after infection of SARS coronavirus (SARS-CoV) with other respiratory viruses including respiratory syncytial virus (RSV), influenza A virus (FluAV), and human parainfluenza virus type 2 (hPIV2). Interferon (IFN) system (production and response) was not suppressed by SARS-CoV infection. Therefore, SARS-CoV replication was suppressed by pretreatment with IFN. SARS-CoV and RSV induced high levels of IL-6 and RANTES compared with FluAV and hPIV2. Induction level of suppressor of cytokine signaling-3 (SOCS3) by SARS-CoV was significantly lower than that by RSV in spite of the significant production of IL-6. Toll-like receptors 4 and 9, which correlate with the induction of inflammatory response, were upregulated by SARS-CoV infection. Collectively, overinduction of inflammatory cytokine and dysregulation of cytokine signaling may contribute to the immunopathology associated with "severe" inflammation in SARS.
Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, Japan.
Tamaki
T
Okabayashi
Hiroaki
H
Kariwa
Shin-ichi
S
Yokota
Shigeo
S
Iki
Tomokazu
T
Indoh
Noriko
N
Yokosawa
Ikuo
I
Takashima
Hiroyuki
H
Tsutsumi
Nobuhiro
N
Fujii
D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D007372:Interferons / Q000235:genetics / Q000378:metabolism*; D012141:Respiratory Tract Infections / Q000276:immunology* / Q000821:virology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000472:pathogenicity*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology* / Q000821:virology; D014777:Virus Diseases / Q000276:immunology* / Q000821:virology; D014779:Virus Replication
D016207:Cytokines; D007372:Interferons