Crystallization and preliminary X-ray analysis of gene product 44 from bacteriophage Mu.
Bacteriophage Mu baseplate protein gene product 44 (gp44) is an essential protein required for the assembly of viable phages. To investigate the roles of gp44 in baseplate assembly and infection, gp44 was crystallized at pH 6.0 in the presence of 20% 2-methyl-2,4-pentanediol. The crystals belong to space group R3, with unit-cell parameters a = b = 127.47, c = 63.97 A. The crystals diffract X-rays to at least 2.1 A resolution and are stable in the X-ray beam and are therefore appropriate for structure determination. Native data have been collected to 2.1 A resolution using a DIP6040 image-plate system at beamline BL44XU at the SPring-8 facility in Japan.
Kondou
Youhei
Y
Institute for Protein Research, Osaka University, Yamada-oka, Suita 565-0871, Japan.
Kitazawa
Daisuke
D
Takeda
Shigeki
S
Yamashita
Eiki
E
Mizuguchi
Mineyuki
M
Kawano
Keiichi
K
Tsukihara
Tomitake
T
D010583:Bacteriophage mu / Q000235:genetics*; D003460:Crystallization; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D014961:X-Ray Diffraction
D011994:Recombinant Proteins; D015678:Viral Structural Proteins