title: Crystallization and preliminary X-ray analysis of alpha-xylosidase from Escherichia coli.
abstract: Glycoside hydrolases have been implicated in many biological processes. To date, they have been classified into 93 glycoside hydrolase (GH) families based on amino-acid sequence similarity. alpha-Xylosidase from Escherichia coli belongs to GH family 31 and catalyzes the release of alpha-xylose from the non-reducing terminal side of alpha-xyloside. Single crystals of alpha-xylosidase have been grown by vapour diffusion at 293 K from 10%(w/v) PEG 20K, 2%(v/v) 2-propanol, 2%(v/v) glycerol and 0.1 M 2-morpholinoethanesulfonic acid pH 5.5. These crystals belong to space group P2(1)2(1)2(1) and X-ray diffraction data were collected to a resolution of 2.75 A. Crystals of selenomethionyl-substituted alpha-xylosidase were also obtained, which diffracted to at least 3.0 A. Based on the value of VM, the asymmetric unit in these crystals was assumed to contain six molecules.
authors.affiliation: Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Momoyo
authors.initials: M
authors.lastname: Kitamura
authors.forename: Toyoyuki
authors.initials: T
authors.lastname: Ose
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okuyama
authors.forename: Hiromi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.forename: Haruhide
authors.initials: H
authors.lastname: Mori
authors.forename: Atsuo
authors.initials: A
authors.lastname: Kimura
authors.forename: Isao
authors.initials: I
authors.lastname: Tanaka
mesh_terms: D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D014961:X-Ray Diffraction; D014995:Xylosidases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D014995:Xylosidases