title: Multiplexing RT-PCR for the detection of multiple miRNA species in small samples.
abstract: MicroRNAs are short (approximately 22 nucleotides), non-coding RNAs that play critical roles in gene regulation and may be used as rapid precise diagnostic indicators of early stages of cancer. The small size of these RNAs makes detection of multiple microRNA species in very small samples problematic. Here we investigate the parameters associated with multiplexing RT-PCR to obtain relative abundance profiles of multiple microRNAs in small sample sizes down to the amount of RNA found in a single cell.
authors.affiliation: Applied Biosystems, 850 Lincoln Centre Dr., Foster City, CA 94404, USA. laokq@appliedbiosystems.com
authors.forename: Kaiqin
authors.initials: K
authors.lastname: Lao
authors.forename: Nan Lan
authors.initials: NL
authors.lastname: Xu
authors.forename: Vivian
authors.initials: V
authors.lastname: Yeung
authors.forename: Caifu
authors.initials: C
authors.lastname: Chen
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Livak
authors.forename: Neil A
authors.initials: NA
authors.lastname: Straus
mesh_terms: D006801:Humans; D008168:Lung / Q000737:chemistry; D035683:MicroRNAs / Q000032:analysis*; D009206:Myocardium / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D035683:MicroRNAs