title: Solution-stirring method improves crystal quality of human triosephosphate isomerase.
abstract: We examined the effect of a solution-stirring method on human triosephosphate isomerase crystallization. The crystals diffracted to more than 1.4 A resolution, whereas those obtained by the normal vapour-diffusion technique diffracted to 2.8 A. These results clearly show that the solution-stirring method is valuable and useful for protein crystallization because of its effectiveness and simplicity.
authors.affiliation: SOSHO Project (Crystal Design Project), Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Adachi
authors.forename: Ai
authors.initials: A
authors.lastname: Niino
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Kinoshita
authors.forename: Masaichi
authors.initials: M
authors.lastname: Warizaya
authors.forename: Riyo
authors.initials: R
authors.lastname: Maruki
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Takano
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Satoshi
authors.initials: S
authors.lastname: Murakami
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Mori
authors.forename: Takatomo
authors.initials: T
authors.lastname: Sasaki
mesh_terms: D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray; D006801:Humans; D012996:Solutions / Q000737:chemistry; D014305:Triose-Phosphate Isomerase / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D012996:Solutions; D014305:Triose-Phosphate Isomerase