title: Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds.
abstract: A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 A resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P2(1)2(1)2(1). Crystallographic refinement and full amino-acid sequence determination are in progress.
authors.affiliation: Department of Biochemistry, Federal University of Ceará, Brazil. bscavada@ufc.br
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.forename: Emmanuel S
authors.initials: ES
authors.lastname: Marinho
authors.forename: Emmanuel P
authors.initials: EP
authors.lastname: Souza
authors.forename: Raquel G
authors.initials: RG
authors.lastname: Benevides
authors.forename: Plínio
authors.initials: P
authors.lastname: Delatorre
authors.forename: Luis A G
authors.initials: LA
authors.lastname: Souza
authors.forename: Kyria S
authors.initials: KS
authors.lastname: Nascimento
authors.forename: Alexandre H
authors.initials: AH
authors.lastname: Sampaio
authors.forename: Frederico B M B
authors.initials: FB
authors.lastname: Moreno
authors.forename: Joane K R
authors.initials: JK
authors.lastname: Rustiguel
authors.forename: Fernanda
authors.initials: F
authors.lastname: Canduri
authors.forename: Walter F
authors.initials: WF
authors.lastname: de Azevedo
authors.forename: Henri
authors.initials: H
authors.lastname: Debray
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D007887:Fabaceae / Q000737:chemistry*; D006384:Hemagglutination; D008358:Mannose / Q000737:chemistry; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits; D012639:Seeds / Q000737:chemistry*
chemical_list: D037121:Plant Lectins; D008358:Mannose