title: Characteristic expression patterns of allatostatin-like peptide, FMRFamide-related peptide, orcokinin, tachykinin-related peptide, and SIFamide in the olfactory system of crayfish Procambarus clarkii.
abstract: The olfactory system plays important roles in various crustacean behaviors. Despite numerous studies on different aspects of the olfactory neural pathway, only the decapod-tachykinin-related peptide (decapod-TRP) has been identified as a neuromodulator in this processing to date. To establish the functions of other related neuropeptides, we initially performed cDNA cloning of FMRFamide-related peptide (FaRP) and allatostatin (AST)-like peptide from the crayfish Procambarus clarkii, followed by in situ hybridization (ISH) analysis of these peptides, along with decapod-TRP, orcokinin, and crustacean-SIFamide. Cloned FaRP cDNA encodes seven copies of C-terminal RN(F/Y)LRFamide-containing peptide, whereas AST-like peptide cDNA comprises 29 copies of AST-like peptide (-YXFGLamide) and three additional putative peptides. ISH analysis of the brain revealed specific expression of crustacean-SIFamide mRNA in most projection neurons (cell cluster 10), and predominant localization of other mRNAs to interneurons. The data suggest that the crustacean-SIFamide neuropeptide is involved in output of the deutocerebrum to the protocerebrum. Double-fluorescence ISH data further disclose that, in cluster 9, orcokinin is coexpressed in decapod-TRP-specific interneurons, whereas AST-like peptide-containing cells do not overlap with orcokinin-expressing cells. On the other hand, FaRP-expressing cells overlap with both orcokinin- and AST-like peptide-specific cells. In cluster 11, where signals for AST-like peptide are absent, a number of interneurons express both decapod-TRP and orcokinin, emphasizing a close relationship between these two factors with regard to olfactory processing, and possibly tactile and/or visual sensory systems. These characteristic expression patterns of neuropeptides support their distinct involvement in the modulation of olfactory processing.
authors.affiliation: Suntory Institute for Bioorganic Research, Wakayamadai, Shimamoto, Mishima, Osaka 618-8503, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Yasuda-Kamatani
authors.forename: Akikazu
authors.initials: A
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000166:cytology / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D019835:FMRFamide / Q000031:analogs & derivatives / Q000235:genetics; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000378:metabolism; D007395:Interneurons / Q000166:cytology / Q000378:metabolism; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009479:Neuropeptides / Q000235:genetics*; D009833:Olfactory Pathways / Q000166:cytology / Q000378:metabolism*; D009842:Oligopeptides / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012903:Smell / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology; D015320:Tachykinins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; C102819:NEB-LFamide; D009479:Neuropeptides; D009842:Oligopeptides; D012333:RNA, Messenger; D015320:Tachykinins; C079067:orcokinin; D019835:FMRFamide