Overview and perspectives the transcriptome of Paracoccidioides brasiliensis.
Paracoccidioides brasiliensis is a dimorphic and thermo-regulated fungus which is the causative agent of paracoccidioidomycosis, an endemic disease widespread in Latin America that affects 10 million individuals. Pathogenicity is assumed to be a consequence of the dimorphic transition from mycelium to yeast cells during human infection. This review shows the results of the P. brasiliensis transcriptome project which generated 6,022 assembled groups from mycelium and yeast phases. Computer analysis using the tools of bioinformatics revealed several aspects from the transcriptome of this pathogen such as: general and differential metabolism in mycelium and yeast cells; cell cycle, DNA replication, repair and recombination; RNA biogenesis apparatus; translation and protein fate machineries; cell wall; hydrolytic enzymes; proteases; GPI-anchored proteins; molecular chaperones; insights into drug resistance and transporters; oxidative stress response and virulence. The present analysis has provided a more comprehensive view of some specific features considered relevant for the understanding of basic and applied knowledge of P. brasiliensis.
Andrade
Rosângela V
RV
Laboratorio de Biologia Molecular, Departamento de Biologia Celular, Universidade de Brasília, Brasilia, DF, 70910-900, Brazil.
Da Silva
Silvana P
SP
Torres
Fernando A G
FA
Poças-Fonseca
Marcio José
MJ
Silva-Pereira
Ildenete
I
Maranhão
Andrea Q
AQ
Campos
Elida G
EG
Moraes
Lídia Maria P
LM
Jesuíno
Rosália S A
RS
Pereira
Maristela
M
Soares
Célia M A
CM
Walter
Maria Emília M T
ME
Carvalho
Maria Joseá A
MJ
Almeida
Nalvo F
NF
Brigido
Marcelo M
MM
Felipe
Maria Sueli S
MS
D002473:Cell Wall / Q000378:metabolism; D048271:Chitosan / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D005800:Genes, Fungal; D016681:Genome, Fungal; D006801:Humans; D007843:Latin America / Q000453:epidemiology; D018832:Molecular Chaperones / Q000235:genetics; D018384:Oxidative Stress / Q000235:genetics; D010228:Paracoccidioides / Q000235:genetics* / Q000648:ultrastructure; D010229:Paracoccidioidomycosis / Q000453:epidemiology / Q000382:microbiology; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics
D005656:Fungal Proteins; D018832:Molecular Chaperones; D048271:Chitosan