title: Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin.
abstract: Human glycosylated haemoglobin A1C is a stable minor variant formed in vivo by post-translational modification of the main form of haemoglobin by glucose. Crystals of oxyHbA1C were obtained using the hanging-drop vapour-diffusion method and PEG as precipitant. The diffraction pattern of the crystal extends to a resolution of 2.3 A at 120 K. The crystals belong to space group C2, with unit-cell parameters a = 237.98, b = 59.27, c = 137.02 A, alpha = 90.00, beta = 125.40, gamma = 90.00 degrees. The presence of two and a half molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 9.70 A3 Da(-1) and a solvent content of 49%.
authors.affiliation: Department of Biochemistry and Biophysics, International Sakharov Environmental University, Dolgobrodskaya St 23, 220009 Minsk, Belarus.
authors.forename: Vitaly E
authors.initials: VE
authors.lastname: Syakhovich
authors.forename: N T
authors.initials: NT
authors.lastname: Saraswathi
authors.forename: Marc
authors.initials: M
authors.lastname: Ruff
authors.forename: Sergey B
authors.initials: SB
authors.lastname: Bokut
authors.forename: Dino
authors.initials: D
authors.lastname: Moras
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006442:Glycated Hemoglobin / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans
chemical_list: D006442:Glycated Hemoglobin