[Yellow laccase from the fungus Pleurotus ostreatus D1: purification and characterization].
Yellow laccase was isolated from a solid-phase culture of the fungus Pleurotus ostreatus D1 and characterized. It is a copper-containing enzyme with molecular weight 64 kDa. Its absorption spectrum lacks the maximum at 610 nm, characteristic of fungal laccases and corresponding to type I copper atom. The optimum pH values for the enzyme were determined. They proved to be: 7.0 for syringaldazine, 8.0 for pyrocatechol, and 4.0 for 2,2'-azine-bis-(3-ethylbenzothiazoline-6-sulfonate and 2,6-dimethoxyphenol. Kinetic parameters (Km and Vmax) for oxidation of these substrates were determined. The effect of inhibitors (SDS, 2-mercaptoethanol, and EDTA) on the activity of the enzyme was studied. It was shown that yellow laccase from Pleurotus ostreatus D1 oxidized anthracene to anthraquinone by 95% without any mediator.
Pozdniakova
N N
NN
Turkovskaia
O V
OV
Iudina
E N
EN
Rodakiewicz-Nowak
Y
Y
D000873:Anthracenes / Q000737:chemistry; D000880:Anthraquinones / Q000737:chemistry; D002384:Catalysis; D002396:Catechols / Q000737:chemistry; D003300:Copper; D004492:Edetic Acid / Q000737:chemistry; D006835:Hydrazones / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000737:chemistry* / Q000302:isolation & purification; D008623:Mercaptoethanol / Q000737:chemistry; D020076:Pleurotus / Q000201:enzymology*; D011748:Pyrogallol / Q000031:analogs & derivatives / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D000873:Anthracenes; D000880:Anthraquinones; D002396:Catechols; D006835:Hydrazones; D011748:Pyrogallol; C009370:syringaldazine; D012967:Sodium Dodecyl Sulfate; C010120:pyrogallol 1,3-dimethyl ether; D008623:Mercaptoethanol; D003300:Copper; D004492:Edetic Acid; D042845:Laccase; C034020:anthracene; C034221:catechol