New Escherichia coli outer membrane proteins identified through prediction and experimental verification.
Many new Escherichia coli outer membrane proteins have recently been identified by proteomics techniques. However, poorly expressed proteins and proteins expressed only under certain conditions may escape detection when wild-type cells are grown under standard conditions. Here, we have taken a complementary approach where candidate outer membrane proteins have been identified by bioinformatics prediction, cloned and overexpressed, and finally localized by cell fractionation experiments. Out of eight predicted outer membrane proteins, we have confirmed the outer membrane localization for five-YftM, YaiO, YfaZ, CsgF, and YliI--and also provide preliminary data indicating that a sixth--YfaL--may be an outer membrane autotransporter.
Marani
Paola
P
Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.
Wagner
Samuel
S
Baars
Louise
L
Genevaux
Pierre
P
de Gier
Jan-Willem
JW
Nilsson
Ingmarie
I
Casadio
Rita
R
von Heijne
Gunnar
G
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002458:Cell Fractionation; D003001:Cloning, Molecular; D019295:Computational Biology / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D020543:Proteome; C057842:SecB protein, Bacteria; C573499:YfaL protein, E coli