Development of dialyzer with immobilized glycoconjugate polymers for removal of Shiga-toxin.
The dialyzer for Shiga-toxin elimination was developed and its performance was established. The dialyzer was prepared by immobilization of multivalent ligands. Glycoconjugate polymers having oligosaccharides and amino groups were synthesized to function as Shiga-toxin adsorbents. The amino group was utilized to immobilize the polymer inside the cellulose hollow fiber of the dialyzer. Cellulose hollow fibers packed in the dialyzer were carboxymethylated under moderate conditions. The glycoconjugate polymers were bound covalently to the hollow fibers of the dialyzer by condensation reaction between the amino group of the polymer and the carboxyl group of the cellulose hollow fiber. Shiga-toxin eliminabilities of the prepared dialyzers were evaluated at various conditions. Even at high concentration of protein such as FCS, the dialyzer showed an excellent performance for Shiga-toxin adsorption.
Miyagawa
Atsushi
A
Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Watanabe
Miho
M
Igai
Katsura
K
Kasuya
Maria Carmelita Z
MC
Natori
Yasuhiro
Y
Nishikawa
Kiyotaka
K
Hatanaka
Kenichi
K
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D015153:Blotting, Western; D002241:Carbohydrates / Q000494:pharmacology; D002522:Chlorocebus aethiops; D003956:Dialysis; D015314:Dialysis Solutions; D004305:Dose-Response Relationship, Drug; D006001:Glycoconjugates / Q000737:chemistry*; D015312:Hemodialysis Solutions; D007700:Kinetics; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D008567:Membranes, Artificial; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry*; D022621:Shiga Toxin / Q000737:chemistry*; D013997:Time Factors; D014709:Vero Cells
D001672:Biocompatible Materials; D002241:Carbohydrates; D015314:Dialysis Solutions; D006001:Glycoconjugates; D015312:Hemodialysis Solutions; D008024:Ligands; D008567:Membranes, Artificial; D011108:Polymers; D022621:Shiga Toxin