Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin.
Human glycosylated haemoglobin A1C is a stable minor variant formed in vivo by post-translational modification of the main form of haemoglobin by glucose. Crystals of oxyHbA1C were obtained using the hanging-drop vapour-diffusion method and PEG as precipitant. The diffraction pattern of the crystal extends to a resolution of 2.3 A at 120 K. The crystals belong to space group C2, with unit-cell parameters a = 237.98, b = 59.27, c = 137.02 A, alpha = 90.00, beta = 125.40, gamma = 90.00 degrees. The presence of two and a half molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 9.70 A3 Da(-1) and a solvent content of 49%.
Syakhovich
Vitaly E
VE
Department of Biochemistry and Biophysics, International Sakharov Environmental University, Dolgobrodskaya St 23, 220009 Minsk, Belarus.
Saraswathi
N T
NT
Ruff
Marc
M
Bokut
Sergey B
SB
Moras
Dino
D
D003460:Crystallization; D018360:Crystallography, X-Ray; D006442:Glycated Hemoglobin / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans
D006442:Glycated Hemoglobin