[Kinetic turbidimetric limulus assay of bacterial endotoxin in the replacement solution of on-line hemodiafiltration].
OBJECTIVE
To determine bacterial endotoxin in the replacement solution of on-line hemodiafiltration (on-line HDF) using kinetic turbidimetric limulus test.


RESULTS
Validation test was performed with the replacement solution of on-line HDF in which quantified standard endotoxin was added. The recovery rates of endotoxin from the replacement solution and its dilutions at 1/5, 1/10, and 1/20 were 58.17%, 106.7%, 99.00% and 98.79%, respectively, suggesting that the optimal dilution was at 1/10. Standard endotoxin was added into the replacement solution of on-line HDF of 3 batches (040408, 040511,040527), and the recovery rates in their dilution at 1/10 were 76.32%, 99.00% and 96.24%, respectively. The standard endotoxin in the working curve was 1.00, 0.125, and 0.0156 Eu/ml (endotoxin unit/ml), and the dilution at 1/10 of the replacement solution is effective to eliminate the interference in limulus test.


CONCLUSIONS
Kinetic turbidimetric limulus test provide a means to detect endotoxin in the replacement solution of on-line HDF.
Lao
Hai-yan
HY
Department of Pharmacy, People's Hospital of Guangdong Province, Guangzhou 510080, China. laohaiyan1208@vip.sina.com
Lin
Qiu-xiao
QX
Yang
Min
M
Liang
Xin-ling
XL
Shi
Wei
W
Li
Hui
H
Feng
Ju-jin
JJ
D004731:Endotoxins / Q000032:analysis*; D017583:Hemodiafiltration / Q000379:methods*; D015312:Hemodialysis Solutions / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D008033:Limulus Test; D009391:Nephelometry and Turbidimetry / Q000379:methods
D004731:Endotoxins; D015312:Hemodialysis Solutions