title: Osteogenic differentiation of human dental papilla mesenchymal cells.
abstract: We isolated dental papilla from impacted human molar and proliferated adherent fibroblastic cells after collagenase treatment of the papilla. The cells were negative for hematopoietic markers but positive for CD29, CD44, CD90, CD105, and CD166. When the cells were further cultured in the presence of beta-glycerophosphate, ascorbic acid, and dexamethasone for 14 days, mineralized areas together with osteogenic differentiation evidenced by high alkaline phosphatase activity and osteocalcin contents were observed. The differentiation was confirmed at both protein and gene expression levels. The cells can also be cryopreserved and, after thawing, could show in vivo bone-forming capability. These results indicate that mesenchymal type cells localize in dental papilla and that the cells can be culture expanded/utilized for bone tissue engineering.
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Research Institute for Cell Engineering (RICE), Tissue Engineering Research Group, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan.
authors.forename: Etsuko
authors.initials: E
authors.lastname: Ikeda
authors.forename: Motohiro
authors.initials: M
authors.lastname: Hirose
authors.forename: Noriko
authors.initials: N
authors.lastname: Kotobuki
authors.forename: Hideki
authors.initials: H
authors.lastname: Shimaoka
authors.forename: Mika
authors.initials: M
authors.lastname: Tadokoro
authors.forename: Masahiko
authors.initials: M
authors.lastname: Maeda
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Kirita
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohgushi
mesh_terms: D002113:Calcification, Physiologic / Q000502:physiology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002648:Child; D003771:Dental Papilla / Q000166:cytology* / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*