title: Preparation, crystallization and preliminary X-ray analysis of YjcG protein from Bacillus subtilis.
abstract: Bacillus subtilis YjcG is a functionally uncharacterized protein with 171 residues that has no structural homologue in the Protein Data Bank. However, it shows sequence homology to bacterial and archaeal 2'-5' RNA ligases. In order to identify its exact function via structural studies, the yjcG gene was amplified from B. subtilis genomic DNA and cloned into the expression vector pET21-DEST. The protein was expressed in a soluble form in Escherichia coli and was purified to homogeneity. Crystals suitable for X-ray analysis were obtained that diffracted to 2.3 A and belonged to space group C2, with unit-cell parameters a = 99.66, b = 73.93, c = 61.77 A, beta = 113.56 degrees.
authors.affiliation: National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Chiomui
authors.initials: C
authors.lastname: Chan
authors.forename: Yu He
authors.initials: YH
authors.lastname: Liang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zheng
authors.forename: Lanfen
authors.initials: L
authors.lastname: Li
authors.forename: Xiao Dong
authors.initials: XD
authors.lastname: Su
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017385:Sequence Homology
chemical_list: D001426:Bacterial Proteins