[Curcumin-induced apoptosis in androgen-dependent prostate cancer cell line LNCaP in vitro].
OBJECTIVE
To explore the apoptosis induction by curcumin in androgen-dependent prostate cancer cell line LNCaP).


METHODS
After LNCaP cells were induced by 10, 25, 50, 75, 100 micromol/L curcumin respectively, the cell activity was assayed by MTT at 5, 12 and 24 hours. Flow cytometry and electronic microscopy were adopted to observe cell cycle and morphological changes of LNCaP cells at 24 hours. After 5 hours, the expression of IkappaBalpha in LNCaP cells was detected by Western blotting.


RESULTS
The growth of LNCaP cells was suppressed obviously by curcumin in dose-dependent and time-dependent manners in vitro. There were significant differences in inhibition rate among different concentrations and time groups (P < 0.05). Furthermore, curcumin could arrest the cell cycle of LNCaP cells at G2/M phase in a dose-dependent manner (P <0.01). The ratios of apoptosis were significantly higher than those of controls (P < 0. 5). Curcumin could lead to characteristic morphological changes of apoptosis in LNCaP cells after 24 hours. The expression of IkappaBalpha in LNCaP cell did not show marked changes after the exposure to different concentrations of curcumin within 5 hours.


CONCLUSIONS
Curcumin can suppress the growth of LNCaP, and promotes their apoptosis.
Guo
Hui
H
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, China. tongjiguohui@yahoo.com.cn
Yu
Jian-Hua
JH
Chen
Ke
K
Ye
Zhang-Qun
ZQ
Liu
Guo-Chang
GC
D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020672:I-kappa B Proteins / Q000096:biosynthesis; D008297:Male; D000072000:NF-KappaB Inhibitor alpha; D009376:Neoplasms, Hormone-Dependent; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*
D020672:I-kappa B Proteins; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha; D003474:Curcumin