title: Cellular unbinding forces of initial adhesion processes on nanopatterned surfaces probed with magnetic tweezers.
abstract: To study the dependence of unbinding forces on the distance of molecularly defined and integrin specific c(-RGDfK-) ligand patches in initial cellular adhesion processes, we developed a magnetic tweezers setup for applying vertical forces of up to 200 pN to rat embryonic fibroblasts. The ligand patch distance is controlled with a hexagonally close packed pattern of biofunctionalized gold nanoparticles prepared by block-copolymer micelle nanolithography. Each gold nanoparticle potentially activates up to one alpha(v)beta(3)-integrin. The distances between the gold nanoparticles determine the separation of individual integrins and thus the assembly of integrin clusters. The results show an increase in cellular unbinding forces from approximately 6 to more than 200 pN for a decreasing ligand distance of 145 to 58 nm after 5 min of cell adhesion. Furthermore, we observe a strong dependence on adhesion time during the first 10 min of cell surface contact suggesting an active, cooperative cell response that is controlled by the spacing between individually activated integrins.
authors.affiliation: Max-Planck-Institute for Metals Research, Department New Materials and Biosystems, Heisenbergstrasse 3, D-70569 Stuttgart, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Walter
authors.forename: Christine
authors.initials: C
authors.lastname: Selhuber
authors.forename: Horst
authors.initials: H
authors.lastname: Kessler
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D005347:Fibroblasts; D016023:Integrins / Q000378:metabolism; D008280:Magnetics; D008854:Microscopy, Electron; D049329:Nanostructures / Q000737:chemistry*; D051381:Rats
chemical_list: D016023:Integrins