[Evidence for the absence of tick-borne encephalitis virus RNA in bioassays].
Reverse transcription-polymerase chain reaction was used to develop a procedure with internal monitoring that may provide evidence for the absence of tick-borne encephalitis virus RNA in bioassays. The proposed procedure is useful in evaluating the safety and effectiveness of a tick-borne encephalitis vaccine.
Romanova
L Iu
LIu
Kozlovskaia
L I
LI
Shevtsova
A S
AS
Karganova
G G
GG
D000818:Animals; D002460:Cell Line; D017931:DNA Primers; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014765:Viral Vaccines / Q000592:standards
D017931:DNA Primers; D012367:RNA, Viral; D014765:Viral Vaccines