Scale-up of L-lactic acid production by mutant strain Rhizopus sp. MK-96-1196 from 0.003 m3 to 5 m3 in airlift bioreactors.
In order to study the feasibility of commercial-scale L-lactic acid production by Rhizopus sp. MK-96-1196 using large scale airlift bioreactors (ALBs), a scale-up study from 0.003 m3 to 5 m3 ALB was carried out using oxygen transfer rate (OTR) as the scale-up criterion. Enhanced L-lactic acid production was achieved at OTRs higher than 0.28 (g-O2/l/h) irrespective of the scale of the bioreactor in question: in the range of 0.003 m3 to 5 m3, more than 90 g/lL-lactic acid was produced with a yield of approximately 80%, based on the initial glucose concentration. In future research, we plan to design an ALB greater than 3000 m3 (working volume: 2000 m3) for further studies on the production of L-lactic acid in large quantities.
Liu
Tiejun
T
The United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Miura
Shigenobu
S
Yaguchi
Masaaki
M
Arimura
Tomohiro
T
Park
Enock Y
EY
Okabe
Mitsuyasu
M
D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D019344:Lactic Acid / Q000096:biosynthesis*; D010100:Oxygen / Q000494:pharmacology; D012233:Rhizopus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D019344:Lactic Acid; D010100:Oxygen