[Polymorphisms analysis of mitochondrial DNA in coding area].
OBJECTIVE
To analyze mitochondrial DNA (mtDNA) polymorphisms in coding area and provide a theoretical basis for applying in forensic science.


METHODS
The primers of 8162F/8483R and 13070F/13299R were designed according to the Anderson's sequence. Using PCR-sequencing method to detect polymorphisms of mtDNA nt8162-8483 and nt13070-13299.


RESULTS
The lengths of the amplicons were 322 bp and 230 bp respectively. There were 24 mitochondrial haplotypes defined by 21 variable positions in both regions. The gene diversity was estimated at 0.751 1, and the probability of two randomly selected individuals having identical mtDNA types was 0.256 4.


CONCLUSIONS
The polymorphic sites within mtDNA coding area can be useful in combination with mtDNA control region in order to increase the discrimination power.
Liu
Ya-cheng
YC
Forensic Medical Examination Center of Beijing Public Security Bureau, Beijing 100085, China. yachenglicc@163.com
Hao
Jin-ping
JP
Yan
Jian-gwei
JG
Tang
Hui
H
Wang
Jing
J
Ren
He
H
Ren
Jia-cheng
JC
D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006238:Haploidy; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004272:DNA, Mitochondrial