title: Development of a process for the production of the anticancer lead compound pleurotin by fermentation of Hohenbuehelia atrocaerulea.
abstract: Pleurotin is a naphthoquinone antibiotic originally isolated from Pleurotus griseus. Two pleurotin producing strains of Hohenbuehelia atrocaerulea have been identified, which, on solid substrate fermentation for 2 months yield 1-2 mg/l of the antibiotic. Described here is the lengthy developmental process which resulted in a production protocol being developed which reliably yields pleurotin from liquid fermentation at >300 mg/l. Critical to obtaining this increase in titer was inclusion in the media of an aqueous extract of alder wood.
authors.affiliation: Natural Products Support Group, SAIC-Frederick, Inc, Bldg. 431, Box B, Frederick, MD 21702, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Shipley
authors.forename: A L
authors.initials: AL
authors.lastname: Barr
authors.forename: S J
authors.initials: SJ
authors.lastname: Graf
authors.forename: R P
authors.initials: RP
authors.lastname: Collins
authors.forename: T G
authors.initials: TG
authors.lastname: McCloud
authors.forename: D J
authors.initials: DJ
authors.lastname: Newman
mesh_terms: D000363:Agaricales / Q000378:metabolism*; D029661:Alnus; D005285:Fermentation; D006576:Heterocyclic Compounds, 4 or More Rings / Q000378:metabolism*; D015394:Molecular Structure; D010936:Plant Extracts / Q000378:metabolism
chemical_list: D006576:Heterocyclic Compounds, 4 or More Rings; D010936:Plant Extracts; C479485:pleurotin