title: Formation of pancreatic duct epithelium from bone marrow during neonatal development.
abstract: Recent reports suggest that bone marrow-derived cells engraft and differentiate into pancreatic tissue at very low frequency after pancreatic injury. All such studies have used adult recipients. The aim of our studies was to investigate the potential of bone marrow to contribute to the exocrine and endocrine components of the pancreas during the normal rapid growth of the organ that occurs during the neonatal period. Five to ten million bone marrow cells from adult, male, transgenic, green fluorescent protein (GFP) mice were injected into neonatal nonobese diabetic/severely compromised immunodeficient/beta2microglobulin-null mice 24 hours after birth. Two months after bone marrow transplantation, pancreas tissue was analyzed with fluorescence immunohistochemistry and fluorescence in situ hybridization (FISH). Co-staining of GFP, with anticytokeratin antibody, and with FISH for the presence of donor Y chromosome indicated that up to 40% of ducts (median 4.6%) contained epithelial cells derived from donor bone marrow. In some of these donor-derived ducts, there were clusters of large and small ducts, all comprised of GFP+ epithelium, suggesting that whole branching structures were derived from donor bone marrow. In addition, rare cells that coexpressed GFP and insulin were found within islets. Unlike pancreatic damage models, no bone marrow-derived vascular endothelial cells were found. In contrast to the neonatal recipients, bone marrow transplanted into adult mice rarely generated ductal epithelium or islet cells (p<.05 difference between adult and neonate transplants). These findings demonstrate the existence in bone marrow of pluripotent stem cells or epithelial precursors that can migrate to the pancreas and differentiate into complex organ-specific structures during the neonatal period.
authors.affiliation: Division of Research Immunology/BMT, Department of Pathology, Childrens Hospital Los Angeles, 4650 Sunset Blvd., M.S. #62, Los Angeles, CA 90027, USA.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wang
authors.forename: Shundi
authors.initials: S
authors.lastname: Ge
authors.forename: Ignacio
authors.initials: I
authors.lastname: Gonzalez
authors.forename: George
authors.initials: G
authors.lastname: McNamara
authors.forename: C Barth
authors.initials: CB
authors.lastname: Rountree
authors.forename: Kenny Kezhe
authors.initials: KK
authors.lastname: Xi
authors.forename: Grace
authors.initials: G
authors.lastname: Huang
authors.forename: Anil
authors.initials: A
authors.lastname: Bhushan
authors.forename: Gay M
authors.initials: GM
authors.lastname: Crooks
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells / Q000502:physiology; D016026:Bone Marrow Transplantation; D007515:Islets of Langerhans / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D008822:Mice, Transgenic; D010179:Pancreas / Q000502:physiology*; D010183:Pancreatic Ducts / Q000166:cytology / Q000502:physiology*; D012038:Regeneration