title: Crystallization and preliminary X-ray analysis of gene product 44 from bacteriophage Mu.
abstract: Bacteriophage Mu baseplate protein gene product 44 (gp44) is an essential protein required for the assembly of viable phages. To investigate the roles of gp44 in baseplate assembly and infection, gp44 was crystallized at pH 6.0 in the presence of 20% 2-methyl-2,4-pentanediol. The crystals belong to space group R3, with unit-cell parameters a = b = 127.47, c = 63.97 A. The crystals diffract X-rays to at least 2.1 A resolution and are stable in the X-ray beam and are therefore appropriate for structure determination. Native data have been collected to 2.1 A resolution using a DIP6040 image-plate system at beamline BL44XU at the SPring-8 facility in Japan.
authors.affiliation: Institute for Protein Research, Osaka University, Yamada-oka, Suita 565-0871, Japan.
authors.forename: Youhei
authors.initials: Y
authors.lastname: Kondou
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kitazawa
authors.forename: Shigeki
authors.initials: S
authors.lastname: Takeda
authors.forename: Eiki
authors.initials: E
authors.lastname: Yamashita
authors.forename: Mineyuki
authors.initials: M
authors.lastname: Mizuguchi
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kawano
authors.forename: Tomitake
authors.initials: T
authors.lastname: Tsukihara
mesh_terms: D010583:Bacteriophage mu / Q000235:genetics*; D003460:Crystallization; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D011994:Recombinant Proteins; D015678:Viral Structural Proteins