Use of natural dye-casein complexes: effect of proteolytic treatment.
The activity of proteolytic enzymes is commonly measured using casein as a substrate. A modified caseinolysis assay was developed with natural dyes such as juglone, lawsone, berberine, and quercetin for Subtilisin carlsberg, protease type XVI, and trypsin, respectively. The pH dependence and incubation time were determined. K(m), V(max), and k(cat)/K(m) values were also determined for these enzymes. Lawsone was found to be a better substrate than the others.
Doğru
Mehmet
M
Department of Chemistry, Faculty of Science Art, Dicle University, Diyarbakir, Turkey.
Baysal
Zübeyde
Z
Aytekin
Cetin
C
Aydin
Haluk
H
D002364:Caseins / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010447:Peptide Hydrolases / Q000737:chemistry*; D013379:Substrate Specificity
D002364:Caseins; D004396:Coloring Agents; D010447:Peptide Hydrolases