In vivo rat model of preeclampsia.
Preeclampsia is characterized by the clinical triad of new hypertension, proteinuria, and edema after 20 wk of gestation. Recent evidence suggests that disturbances in angiogenic factors (such as vascular endothelial growth factor and placental growth factor) signaling and endothelial health may play a major role in the pathogenesis of preeclampsia. Exogenous administration of soluble fms-like tyrosine kinase-1 (sFlt-1; a circulating anti-angiogenic protein) results in a preeclampsia-like phenotype in rats. This chapter describes the methods we use in our laboratory to create the sFlt-1 induced-rat model of preeclampsia.
Karumanchi
S Ananth
SA
Department of Medicine, Obstetrics and Gynecology, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, MA, USA.
Stillman
Isaac E
IE
D000256:Adenoviridae; D000818:Animals; D001794:Blood Pressure; D004195:Disease Models, Animal; D005260:Female; D007668:Kidney / Q000473:pathology; D008854:Microscopy, Electron; D011225:Pre-Eclampsia / Q000473:pathology / Q000503:physiopathology* / Q000652:urine; D011247:Pregnancy; D011507:Proteinuria / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000502:physiology*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood / Q000502:physiology*
C501164:Flt1 protein, rat; D040281:Vascular Endothelial Growth Factor Receptor-1