Application of a fast high-performance liquid chromatography method for simultaneous determination of furanic compounds and glucosylisomaltol in breakfast cereals.
The high-performance liquid chormatography method reported in the present paper provides a fast, low-cost, precise, and simple technique to analyze simultaneously 3 different indicators of nonenzymatic browning, i.e., hydroxymethylfurfural (HMF), furfural, and glucosylisomaltol (GIM), in breakfast cereals. These compounds were extracted in aqueous solution and separated on a reversed-phase C18 column with water-acetonitrile (95 + 5, v/v) mobile phase under isocratic conditions. Average recovery rates for HMF, furfural, and GIM were 99.1, 98.4, and 99.4%, respectively. The coefficients of variation were 3.67, 2.42, and 1.59% for HMF, furfural, and GIM, respectively. The detection limit was 0.01 mg/kg, and the quantitation limit was 0.05 mg/kg for the 3 studied compounds.
Rufián-Henares
Jose A
JA
Consejo Superior de Investigaciones Cientificas, Instituto del Frío, José Antonio Nováis 10, E-28040 Madrid, Spain.
Delgado-Andrade
Cristina
C
Morales
Francisco J
FJ
D000097:Acetonitriles / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D002523:Edible Grain / Q000378:metabolism*; D005504:Food Analysis / Q000379:methods*; D005663:Furans / Q000737:chemistry*; D007534:Isomaltose / Q000031:analogs & derivatives* / Q000032:analysis; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D012964:Sodium / Q000737:chemistry; D014867:Water
D000097:Acetonitriles; D005663:Furans; C470212:glucosylisomaltol; D014867:Water; D007534:Isomaltose; D012964:Sodium; C039281:furan; C032159:acetonitrile