title: Crystallization and preliminary crystallographic analysis of endo-1,3-beta-glucanase from Arthrobacter sp.
abstract: Endo-1,3-beta-glucanases hydrolyze internal 1,3-beta-glucosyl linkages. The endo-1,3-beta-glucanase from Arthrobacter sp. was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group P4(1), with unit-cell parameters a = 71.31, c = 60.07 A, and contained one molecule per asymmetric unit. The Matthews coefficient (VM) and the solvent content were 2.35 A3 Da(-1) and 47.63%, respectively. Diffraction data were collected to a resolution of 1.66 A at SPring-8 using a MAR CCD area detector and gave a data set with an overall Rmerge of 5.4% and a completeness of 99.4%.
authors.affiliation: Graduate School of Agriculture, Kyoto Prefectural University, 1-5 Shimogamo, Kyoto 606-8522, Japan.
authors.forename: Zhongcun
authors.initials: Z
authors.lastname: Pang
authors.forename: You-Na
authors.initials: YN
authors.lastname: Kang
authors.forename: Mizuho
authors.initials: M
authors.lastname: Ban
authors.forename: Masayuki
authors.initials: M
authors.lastname: Oda
authors.forename: Ryo
authors.initials: R
authors.lastname: Kobayashi
authors.forename: Masatake
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Bunzo
authors.initials: B
authors.lastname: Mikami
mesh_terms: D001173:Arthrobacter / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D018360:Crystallography, X-Ray; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D008958:Models, Molecular; D017433:Protein Structure, Secondary
chemical_list: D001426:Bacterial Proteins; D006026:Glycoside Hydrolases; C028020:licheninase