Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I.
Crystals of site-specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 A resolution at 100 K. The crystal belongs to space group P2(1), with unit-cell parameters a = 57.76, b = 90.67, c = 71.71, beta = 110.1 degrees. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume.
Kachalova
Galina S
GS
Max-Planck Research Unit for Structural Biology, Protein Dynamics Group, Hamburg 22607, Germany. galina@mpghdb.desy.de
Rogulin
Eugeny A
EA
Artyukh
Rimma I
RI
Perevyazova
Tatyana A
TA
Zheleznaya
Ludmila A
LA
Matvienko
Nickolay I
NI
Bartunik
Hans D
HD
D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D013379:Substrate Specificity; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D003850:Deoxyribonuclease I