title: Tissue engineering of articular cartilage with autologous cultured adipose tissue-derived stromal cells using atelocollagen honeycomb-shaped scaffold with a membrane sealing in rabbits.
abstract: Adipose tissue derived stromal cells (ATSCs), which were isolated from adipose tissue of rabbit, have shown to possess multipotential, that is, they differentiate into osteoblasts and adipocytes in plate-culturing and into chondrocytes in an established aggregate culture using defined differentiation-inductive medium. The aim of this study was to evaluate the utility of ATSCs in tissue engineering procedures for repair of articular cartilage-defects using the atelocollagen honeycomb-shaped scaffold with a membrane sealing (ACHMS-scaffold). We intended to repair full-thickness articular cartilage defects in rabbit knees using autologously cultured ATSCs embedded in the ACHMS-scaffold. ATSCs were incubated within the ACHMS-scaffold to allow a high density and three-dimensional culture with control medium. An articular cartilage defect was created on the patellar groove of the femur, and the defect was filled with the ATSCs-containing ACHMS-scaffold, ACHMS-scaffold alone, or empty (control). Twelve weeks after the operation, the histological analyses showed that only the defects treated with the ATSCs-containing ACHMS-scaffold were filled with reparative hyaline cartilage, highly expressed Type II collagen. These results indicate that transplantation of autologous ATSCs-containing ACHMS-scaffold is effective in repairing articular cartilage defects.
authors.affiliation: Department of Orthopaedic Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Masuoka
authors.forename: Takashi
authors.initials: T
authors.lastname: Asazuma
authors.forename: Hidemi
authors.initials: H
authors.lastname: Hattori
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshihara
authors.forename: Masato
authors.initials: M
authors.lastname: Sato
authors.forename: Kouji
authors.initials: K
authors.lastname: Matsumura
authors.forename: Takemi
authors.initials: T
authors.lastname: Matsui
authors.forename: Bonpei
authors.initials: B
authors.lastname: Takase
authors.forename: Koichi
authors.initials: K
authors.lastname: Nemoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002358:Cartilage, Articular; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003094:Collagen; D008297:Male; D011817:Rabbits; D012038:Regeneration / Q000502:physiology; D017154:Stromal Cells; D023822:Tissue Engineering
chemical_list: C059821:atelocollagen; D003094:Collagen