title: Crystallization and initial crystal characterization of the C-terminal phosphoglycerate mutase homology domain of Sts-1.
abstract: Sts-1 is a multidomain protein that plays an important role in T-cell signaling. Sts-1 contains a ubiquitin-association (UBA) domain at the N-terminus, followed by an Src homology-3 (SH3) domain and a C-terminal domain that shares sequence homology to phosphoglycerate mutases (PGMs). The C-terminal domain of Sts-1, Sts-1(PGM), crystallizes in space group C2 with two different crystal forms. The first crystal form contains two or three Sts-1PGM molecules in the asymmetric unit and diffracts to 1.82 A resolution, with unit-cell parameters a = 116.2, b = 74.3, c = 100.1 A, alpha = gamma = 90, beta = 101.5 degrees. The second crystal form contains four or six Sts-1(PGM) molecules in the asymmetric unit, with unit-cell parameters a = 214.9, b = 75.1, c = 116.4 A, alpha = gamma = 90, beta = 111.6 degrees. Greater than 95% complete native and SeMet data sets have been collected and structure determination using the multiple anomalous dispersion (MAD) technique is ongoing.
authors.affiliation: Department of Physiology and Biophysics, Basic Sciences Tower, Stony Brook University, Stony Brook, NY 11794-8661, USA.
authors.forename: Holly
authors.initials: H
authors.lastname: Kleinman
authors.forename: Bradley
authors.initials: B
authors.lastname: Ford
authors.forename: James
authors.initials: J
authors.lastname: Keller
authors.forename: Nick
authors.initials: N
authors.lastname: Carpino
authors.forename: Nicolas
authors.initials: N
authors.lastname: Nassar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D051379:Mice; D008969:Molecular Sequence Data; D010736:Phosphoglycerate Mutase / Q000737:chemistry*; D017027:Protein Tyrosine Phosphatases; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry*; D012645:Selenomethionine; D016415:Sequence Alignment
chemical_list: D011948:Receptors, Antigen, T-Cell; D012645:Selenomethionine; D017027:Protein Tyrosine Phosphatases; C553497:TULA-2 protein, mouse; D010736:Phosphoglycerate Mutase