Influence of Cu(II) on the interaction of sulfite with DNA.
The quantitative influence of Cu(II) on the interaction of eukaryotic DNA with sulfite (SO(3)(2-)), which is a derivative of sulfur dioxide in the human body, was studied using ultraviolet (UV) absorption spectrometry. The results showed that under physiological pH conditions, SO(3)(2-) reacted weakly with DNA at concentrations of up to 10(-1)M, at which point a rapid increase in the reaction constant and the reaction number of SO(3)(2-) with DNA was observed. The addition of Cu(II) at concentrations ranging from 6.67 x 10(-4) to 3.33 x 10(-3)M to DNA-SO(3)(2-) binary systems increased the reaction constant of SO(3)(2-) with DNA 41- to 115-fold at a low concentration of SO(3)(2-) (10(-3)M), and 4- to 84-fold at an intermediate concentration of SO(3)(2-) (10(-2)M), but had little influence on the reaction number of SO(3)(2-) with DNA compared with the absence of Cu(II). When the concentration of SO(3)(2-) reached 10(-1)M, the presence of Cu(II) reduced the reaction number but had no effect on the reaction constant of SO(3)(2-) with DNA. These results show that the efficiency of SO(3)(2-) is increased in the presence of Cu(II) at high concentrations of SO(3)(2-).
Guo
Dong-Sheng
DS
College of Environment and Resources, Shanxi University, Taiyuan 030006, China. dongsguo@sxu.edu.cn
Yuan
Xiao-Ying
XY
Liang
Jie-Qing
JQ
D000393:Air Pollutants / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D003300:Copper / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects*; D018736:DNA Adducts; D004249:DNA Damage; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D013056:Spectrophotometry, Ultraviolet; D013447:Sulfites / Q000494:pharmacology*; D013458:Sulfur Dioxide / Q000494:pharmacology*
D000393:Air Pollutants; D018736:DNA Adducts; D013447:Sulfites; D013458:Sulfur Dioxide; D003300:Copper; D004247:DNA; C025026:sodium sulfite