title: A rapid LC-MS method for determination of plasma anion profiles of acidotic patients.
abstract: In metabolic acidosis, the concentrations of anions associated with intermediary metabolism are increased and can make a significant contribution to the observed acidosis. Here we describe a method for the rapid determination of the plasma ultrafiltrate profile of these anions using liquid chromatography coupled to electrospray ionisation mass spectrometry (LC/ESI-MS). The ultrafiltrate from patients with acidosis resulting from various causes were examined and the results compared to control values. Using the LC/ESI-MS method described, a unique plasma ultrafiltrate anion profile was obtained for each of the groups studied that provides rapid diagnosis of the type of underlying acidosis.
authors.affiliation: Renal Research Laboratory, Department Medicine, 4th Floor, North Wing, St. Thomas' Hospital, Lambeth Palace Road, London SE1 7EH, UK.
authors.forename: William
authors.initials: W
authors.lastname: McKinnon
authors.forename: Gwyn A
authors.initials: GA
authors.lastname: Lord
authors.forename: Lui G
authors.initials: LG
authors.lastname: Forni
authors.forename: Jean-Marie R
authors.initials: JM
authors.lastname: Peron
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Hilton
mesh_terms: D000138:Acidosis / Q000097:blood*; D000838:Anions / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000838:Anions