Bisulfite-modified target DNA array for aberrant methylation analysis.
Aberrant DNA methylation of CpG islands is among the earliest and most frequent alterations in cancer. It is of great importance to develop simple and high-throughput methods of methylation analysis for earlier cancer diagnosis or the detection of recurrence. In this study, bisulfite-modified target DNA arrays were prepared on positively charged nylon membrane with two different procedures: fixing PCR products and fixing genomic DNA. First, a bisulfite PCR product array was prepared through fixing PCR products amplified in bisulfite sequencing primers from the bisulfite-modified genomic DNA of different clinical samples on membrane. Furthermore, bisulfite-modified genomic DNA of the different samples was directly fixed on membrane to fabricate bisulfite genomic DNA arrays. The two kinds of arrays were hybridized by probes labeled with digoxigenin, and the hybridization signals were obtained through chemiluminescent detection. The methylation statuses of the IGFBP7 gene for breast tumor and normal tissue samples and for normal human blood cell samples were detected successfully by the two procedures. It was shown that the methods are reliable and sensitive and that they have high potential in screening molecular methylation markers from a large number of clinical samples.
Zhou
Dongrui
D
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
Qiao
Wanqiong
W
Yang
Liguo
L
Lu
Zuhong
Z
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation; D017931:DNA Primers; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D013447:Sulfites / Q000737:chemistry*
D017931:DNA Primers; D013447:Sulfites; D004247:DNA