title: Polymerase chain reaction in miniaturized systems: big progress in little devices.
abstract: A critical need exists for advanced technologies that enable genomic-based DNA analysis to be performed with significantly higher throughput and at a significantly lower cost than is attainable with current hardware. Miniaturized polymerase chain reaction systems offer an attractive platform to address these needs, combining the ability to perform rapid thermocycling with a portable device format that can be inexpensively mass produced. We review recent efforts aimed at developing these next-generation systems and discuss some of the practical considerations involved in adapting this technology for routine laboratory use.
authors.affiliation: Department of Chemical Engineering, Texas A&M University, College Station, USA.
authors.forename: Kalee D
authors.initials: KD
authors.lastname: Spitzack
authors.forename: Victor M
authors.initials: VM
authors.lastname: Ugaz
mesh_terms: D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods* / Q000639:trends; D044085:Microfluidics; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods* / Q000639:trends