The dynamic nature of mollusc egg surface architecture and its relation to the microtubule network.
Dynamic changes in the surface architecture pattern of embryos of the slipper limpet (Crepidula fornicata, Mollusca) were found in this study to correlate with the dynamic activity and pattern of the underlying mitotic spindle microtubule network, revealed by fluorescent labelling and confocal imaging techniques. Examination of a series of optical sections indicate that this network appears to be spatially co-ordinated together as a whole throughout the embryo. The microtubule pattern also associated with abnormal multipolar spindles resulting from an applied static magnetic field, indicating that the pattern may be generated by a natural endogenous field source. The patterning characteristics of the surface and microtubule network together provide further morphological evidence for a primary morphogenetic or developmental field system which organises the primary body axis and co-ordinates the pattern of cleavage.
Tyler
Sheena E B
SE
Faculty of Life Sciences, University of Manchester, England. sheena.tyler@man.ac.uk
Kimber
Susan J
SJ
D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002970:Cleavage Stage, Ovum / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000502:physiology; D005260:Female; D008280:Magnetics; D008297:Male; D008870:Microtubules / Q000502:physiology*; D008974:Mollusca / Q000196:embryology / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D008941:Spindle Apparatus / Q000502:physiology; D013499:Surface Properties