X-ray crystallographic characterization of rhesus macaque MHC Mamu-A*02 complexed with an immunodominant SIV-Gag nonapeptide.
Simian immunodeficiency virus (SIV) in the rhesus macaque is regarded as a classic animal model, playing a crucial role in HIV vaccine strategies and therapeutics by characterizing various cytotoxic T-lymphocyte (CTL) responses in macaque monkeys. However, the availability of well documented structural reports focusing on rhesus macaque major histocompatibility complex class I (MHC I) molecules remains extremely limited. Here, a complex of the rhesus macaque MHC I molecule (Mamu-A*02) with human beta2m and an immunodominant SIV-Gag nonapeptide, GESNLKSLY (GY9), has been crystallized. The crystal diffracts X-rays to 2.7 A resolution and belongs to space group C2, with unit-cell parameters a = 124.11, b = 110.45, c = 100.06 A, and contains two molecules in the asymmetric unit. The availability of the structure, which is being solved by molecular replacement, will provide new insights into rhesus macaque MHC I (Mamu-A*02) presenting pathogenic SIV peptides.
Feng
Youjun
Y
Laboratory of Molecular Immunology and Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Qi
Jianxun
J
Zhang
Huimin
H
Wang
Jinzi
J
Liu
Jinhua
J
Jiang
Fan
F
Gao
Feng
F
D000818:Animals; D017951:Antigen Presentation; D003460:Crystallization; D018360:Crystallography, X-Ray; D015683:Gene Products, gag / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D016056:Immunodominant Epitopes / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008253:Macaca mulatta; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D015302:Simian Immunodeficiency Virus / Q000737:chemistry* / Q000276:immunology*
D015683:Gene Products, gag; D015395:Histocompatibility Antigens Class I; D016056:Immunodominant Epitopes; C089390:Mamu-A 02 antigen; D010446:Peptide Fragments