title: Oocyte maturity and preimplantation development in relation to follicle diameter in gonadotropin-releasing hormone agonist or antagonist treatments.
abstract: OBJECTIVE
  To analyze the distribution of oocytes nuclear maturational stages and intracytoplasmic sperm injection (ICSI) outcome in relation to follicular size, after controlled ovarian hyperstimulation (COH), using gonadotropin-releasing hormone (GnRH) antagonist or agonist protocols.
  
  
  METHODS
  Prospective comparative study.
  
  
  METHODS
  Fertility unit in an academic hospital.
  
  
  METHODS
  Normo-ovulatory patients with unexplained, male and/or tubal factor infertility undergoing ICSI treatment.
  
  
  METHODS
  Oocytes were retrieved from small (7-12 mm) and large follicles (>12 mm).
  
  
  METHODS
  Oocyte nuclear maturation stage, fertilization and pre-implantation embryonic development of the mature ova evaluated.
  
  
  RESULTS
  Distribution of oocyte maturation stages from small follicles did not differ between the two COH protocols. From large follicles, a statistically significantly higher percentage of immature oocytes was retrieved after antagonist compared with agonist treatment (16 vs 11%, respectively). No statistically significant differences existed regarding ICSI outcome of mature ova within the same follicle diameter range between COH protocols. Inseminated ova from small follicles presented a significantly higher number of embryos with delayed development on day 3 after ICSI.
  
  
  CONCLUSIONS
  A greater heterogeneity of maturity was found in the population of oocyte stages that were present after the antagonist protocol. Matured oocytes retrieved from small follicles generated embryos of lower developmental potential than oocytes derived from larger follicles.
authors.affiliation: Follicle Biology Laboratory, Free University of Brussels, Brussels, Belgium. daniela_nogueira@yahoo.com
authors.forename: Daniela
authors.initials: D
authors.lastname: Nogueira
authors.forename: Shevach
authors.initials: S
authors.lastname: Friedler
authors.forename: Morey
authors.initials: M
authors.lastname: Schachter
authors.forename: Arieh
authors.initials: A
authors.lastname: Raziel
authors.forename: Raphael
authors.initials: R
authors.lastname: Ron-El
authors.forename: Johan
authors.initials: J
authors.lastname: Smitz
mesh_terms: D000328:Adult; D001755:Blastocyst; D016922:Cellular Senescence; D047108:Embryonic Development; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists* / Q000037:antagonists & inhibitors*; D006801:Humans; D007246:Infertility / Q000473:pathology / Q000628:therapy*; D009865:Oocytes; D009929:Organ Size; D006080:Ovarian Follicle / Q000473:pathology*; D011446:Prospective Studies; D016037:Single-Blind Method; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D007987:Gonadotropin-Releasing Hormone