[Analysis of the secreted proteins encoded by genes in genome of filamental fungus (Neurospora crassa)].
The completed genome sequence of filamental fungus Neurospora crassa has completed and 10,082 open reading frames (ORFs) were predicted in whole genome. Among them, 437 proteins were secreted ones based on the combination of prediction algorithms SignalP v3.0 and PSORT, transmembrane domains prediction algorithms TMHMM v2.0 and THUMBUP, potential GPI-anchor sites prediction algorithm big-PI predictor and the subcellular protein location prediction algorithm TargetP v1.01. The minimum ORF length of the encoding sequences for 437 secreted proteins was 252 bp, the maximum ORF length was 6 604 bp and average was 1 433 bp. The length of signal peptide of secreted proteins ranged from 15 to 59 amino acids. Among the 437 secreted proteins, 205 were provided with function description. The function of secretory proteins were involved in kinds of enzyme, cell energy, transmission, cell recovered itself and defense mechanism et al. These results implied that processes catalyzed by these proteins probably occurred outside of cells, at least partially.
Zhou
Xiao-Gang
XG
Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China. zxg88@163.com
Li
Cheng-Yun
CY
Zhao
Zhi-Wei
ZW
Su
Yuan
Y
Zhang
Shao-Song
SS
Li
Jin-Bin
JB
Yang
Jing
J
Liu
Lin
L
Lin
Yan-Fen
YF
D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics* / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000235:genetics
D005656:Fungal Proteins; D021382:Protein Sorting Signals