title: Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers.
abstract: We show herein that interaction in aqueous solution of the two components of binary toxin from Bacillus sphaericus, BinA and BinB, leads to a dramatic conformational change, from beta turns or random coil, to beta structure. Also, either BinA or BinB separately or their equimolar mixture, interact with lipid bilayers resulting in further conformational changes. Upon membrane association, the change in conformation observed for BinA or BinB separately is different from that observed when the proteins are combined, indicating that proper folding depends on the presence of the complementary subunit. We also show, in contrast to previous reports, that BinB, but not BinA, is able to insert in model neutral lipid monolayers.
authors.affiliation: Institute of Molecular Biology and Genetics, Mahidol University, Salaya, Phuttamonthol, Nakornpathom 73170, Thailand. mbpbs@mahidol.ac.th
authors.forename: Panadda
authors.initials: P
authors.lastname: Boonserm
authors.forename: Seangduen
authors.initials: S
authors.lastname: Moonsom
authors.forename: Chanikarn
authors.initials: C
authors.lastname: Boonchoy
authors.forename: Boonhiang
authors.initials: B
authors.lastname: Promdonkoy
authors.forename: Krupakar
authors.initials: K
authors.lastname: Parthasarathy
authors.forename: Jaume
authors.initials: J
authors.lastname: Torres
mesh_terms: D001427:Bacterial Toxins / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008565:Membrane Proteins / Q000737:chemistry*; D011487:Protein Conformation; D012996:Solutions
chemical_list: D001427:Bacterial Toxins; D008051:Lipid Bilayers; D008565:Membrane Proteins; D012996:Solutions; C046134:binB protein, Bacillus sphaericus