Joint effects of microwave and chromium trioxide on root tip cells of Vicia faba.
The mutagenic effects of microwave and chromium trioxide (CrO(3)) on Vicia faba root tip were studied. Micronucleus assay and chromosomal aberration assay were used to determine the mitotic index, the micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells induced by microwave and CrO(3). The results showed that the micronucleus frequency decreased, and that the mitotic index and chromosomal aberration frequency showed linear dose responses to CrO(3), in treatment of microwave for 5 s. In microwave of 25 s, the mitotic index decreased, the micronucleus frequency and chromosomal aberration frequency increased with increase of CrO(3) concentration. We concluded that microwave and CrO(3) had antagonistic effect on the mitotic index of Vicia faba root tip cells, but had synergetic effect on micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells.
Qian
Xiao-wei
XW
School of Life and Environmental Science, Wenzhou University, Wenzhou 325003, China. qianxiaowei@126.com
Luo
Wei-hua
WH
Zheng
Ou-xiang
OX
D002478:Cells, Cultured; D017608:Chromium Compounds / Q000633:toxicity*; D002869:Chromosome Aberrations / Q000187:drug effects / Q000528:radiation effects; D032461:Chromosomes, Plant / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D015162:Micronucleus Tests; D008872:Microwaves / Q000009:adverse effects*; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D031307:Vicia faba / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*
D017608:Chromium Compounds; C028801:chromium trioxide