title: Mitochondrial DNA deletion in granulosa and cumulus oophorus cells.
abstract: The incidence of the 4,977-bp deletion in mitochondrial DNA (DeltamtDNA4977) in 73 pairs of granulosa and cumulus oophorus cells was studied with polymerase chain reaction (PCR), and was significantly higher in granulosa cells (GC) (67/73, 91%) than in cumulus oophorus cells (17/73, 23.3%), independent of the donors' age. In the cumulus oophorus cells, the oocyte morphology, the ooplasma diameter, and the proportion of oocytes fertilized normally were comparable between those with and without DeltamtDNA4977; whereas the oocyte diameter and the zona thickness were significantly higher in those with DeltamtDNA4977.
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Hong Kong. cwcchan@graduate.hku.hk
authors.forename: Carina C W
authors.initials: CC
authors.lastname: Chan
authors.forename: Vincent W S
authors.initials: VW
authors.lastname: Liu
authors.forename: Estella Y L
authors.initials: EY
authors.lastname: Lau
authors.forename: William S B
authors.initials: WS
authors.lastname: Yeung
authors.forename: Ernest H Y
authors.initials: EH
authors.lastname: Ng
authors.forename: Pak-Chung
authors.initials: PC
authors.lastname: Ho
mesh_terms: D000328:Adult; D000375:Aging / Q000502:physiology; D004272:DNA, Mitochondrial; D005260:Female; D017353:Gene Deletion; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D009865:Oocytes / Q000648:ultrastructure*; D006080:Ovarian Follicle / Q000166:cytology* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D015044:Zona Pellucida / Q000648:ultrastructure
chemical_list: D004272:DNA, Mitochondrial