Crystallization and preliminary crystallographic analysis of the nickel-responsive regulator NikR from Pyrococcus horikoshii.
The nickel-responsive repressor from Pyrococcus horikoshii OT3 (PhNikR) has been crystallized in the apo form (PhNikR-apo) and two nickel-bound forms (PhNikR-Ni-1 and PhNikR-Ni-2). The PhNikR-apo crystals belong to space group P2(1), with unit-cell parameters a = 75.78, b = 54.32, c = 77.28 A, beta = 116.07 degrees, and diffract to 2.2 A. The PhNikR-Ni-1 crystals belong to space group P4(1)2(1)2, with unit-cell parameters a = b = 99.89, c = 97.98 A, and diffract to 3.0 A and the PhNikR-Ni-2 crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 109.95, c = 79.0 A, and diffract to 2.1 A. The crystals obtained were suitable for detailed structural studies.
Kitao
Tomoe
T
Highthroughput Factory, RIKEN Harima Institute, 1-1-1 Kouto, Hyogo 679-5148, Japan.
Kuroishi
Chizu
C
Tahirov
Tahir H
TH
D019843:Archaeal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D003460:Crystallization; D009532:Nickel / Q000494:pharmacology; D043902:Pyrococcus horikoshii / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D014961:X-Ray Diffraction
D019843:Archaeal Proteins; D011994:Recombinant Proteins; D012097:Repressor Proteins; D009532:Nickel