title: In vitro models for studying trophoblast transcellular transport.
abstract: In vitro models have proven to be effective in studying the placental transporters that play a role in the exchange of nutrients, waste products, and drugs between the maternal and fetal circulations. Although primary cultures of trophoblast cells can be used to perform uptake, efflux, and metabolism studies, only the rodent HRP-1 and the human BeWo cell lines have been shown to form confluent monolayers when grown on semi-permeable membranes. Protocols for the revival, maintenance, passage, and growth of BeWo cells for transporter expression and transcellular transport studies are provided.
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas, Lawrence, USA.
authors.forename: Claudia J
authors.initials: CJ
authors.lastname: Bode
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.forename: Erik
authors.initials: E
authors.lastname: Rytting
authors.forename: Peter S
authors.initials: PS
authors.lastname: Silverstein
authors.forename: Amber M
authors.initials: AM
authors.lastname: Young
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Audus
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor / Q000378:metabolism*; D002822:Choriocarcinoma; D005260:Female; D006801:Humans; D026901:Membrane Transport Proteins / Q000378:metabolism; D008954:Models, Biological; D010539:Permeability; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: D026901:Membrane Transport Proteins