[DNA extraction from old bones].
OBJECTIVE
To explore an efficient method for extracting DNA from old bones.


METHODS
Using an organic combined with and Microcon 100 to extract and purify DNA.


RESULTS
The extracted DNA was successfully genotyped by using florescence labeling STR multiplex amplification.


CONCLUSIONS
This method will be useful for forensic scientists in identification of DNA from old bones.
Zhang
Jie
J
Criminal Police Department of Taian City Public Security Bureau, Taian 271000, China. zxingda@163.com
Xue
Qing-jie
QJ
Tang
Guang-feng
GF
Peng
Lei
L
D001842:Bone and Bones / Q000737:chemistry*; D002102:Cadaver; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D005269:Femur / Q000737:chemistry; D018732:Forensic Anthropology / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D020080:Tandem Repeat Sequences
D004247:DNA