title: [HLA-DPB1 gene analysis in haematopoietic stem cell transplantations].
abstract: BACKGROUND
  The HLA-DPB1 gene is probably one of HLA class II genes affecting the haematopoietic stem cell transplantation. The aim of the study was to analyse the HLA-DPB1 gene and its match/mismatch in patients transplanted from unrelated HSC donors. The PCR-SSP method was used for the typing of the HLA system.
  
  
  RESULTS
  The cohort consisted of 201 pairs of patient/unrelated HSC donor. Match in HLA-A, -B, -Cw, -DRBI and -DQBI (e.g. 10/10 match) was found in 81 pairs. The HLA-DPBI was tested in them. 18 different HLA-DPBI alleles were identified in this cohort. Complete match (e.g.12/12) was detected in 3% of the 201 analysed pairs only.
  
  
  CONCLUSIONS
  The probability of finding 12/12 matching unrelated HSC donor is limited due to the high percentage of mismatches and inaccessibility of previous HLA-DPB1 results.
authors.affiliation: Ustav hematologie a krevní transfuze, Praha. milena.vrana@uhkt.cz
authors.forename: M
authors.initials: M
authors.lastname: Vraná
authors.forename: M
authors.initials: M
authors.lastname: Dobrovolná
authors.forename: P
authors.initials: P
authors.lastname: Cetkovský
authors.forename: S
authors.initials: S
authors.lastname: Nazarová
authors.forename: H
authors.initials: H
authors.lastname: Vondrácková
authors.forename: P
authors.initials: P
authors.lastname: Sedlácek
mesh_terms: D000328:Adult; D002648:Child; D005787:Gene Frequency; D006682:HLA-DP Antigens / Q000235:genetics*; D059868:HLA-DP beta-Chains; D018380:Hematopoietic Stem Cell Transplantation; D006650:Histocompatibility Testing; D006801:Humans; D014019:Tissue Donors
chemical_list: D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen