Simultaneous quantification of methamphetamine, cocaine, codeine, and metabolites in skin by positive chemical ionization gas chromatography-mass spectrometry.
A positive chemical ionization gas chromatography-mass spectrometric method was validated to simultaneously quantify drugs and metabolites in skin collected after controlled administration of methamphetamine, cocaine, and codeine. Calibration curves (2.5-100 ng/skin biopsy) for methamphetamine, amphetamine, cocaine, norcocaine, benzoylecgonine, cocaethylene, norcocaethylene, anhydroecgonine methyl ester, morphine, codeine, and 6-acetylmorphine (5-100 ng/skin biopsy for ecgonine methyl ester and ecgonine ethyl ester) exhibited correlation coefficients >0.999 and concentrations +/-20% of target. Intra- and inter-run precisions were <10%. This procedure should be useful for postmortem analysis; data are included on drug concentrations in skin after controlled drug administration.
Yang
Wonkyung
W
National Institute of Scientific Investigation, 331-1 Shinwol 7-dong, Yangcheon-Gu, Seoul 158-707, Republic of Korea.
Barnes
Allan J
AJ
Ripple
Mary G
MG
Fowler
David R
DR
Cone
Edward J
EJ
Moolchan
Eric T
ET
Chung
Heesun
H
Huestis
Marilyn A
MA
D002138:Calibration; D003042:Cocaine / Q000378:metabolism*; D003061:Codeine / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008694:Methamphetamine / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012867:Skin / Q000378:metabolism*
D008694:Methamphetamine; D003042:Cocaine; D003061:Codeine