title: [Bioinformatics analysis of YZ-2 and preparation of polyclonal antibody].
abstract: OBJECTIVE
  To analyze YZ-2, a novel protein associated with cerebral ischemia and prepares its polyclonal antibody.
  
  
  METHODS
  According to the bioinformatics analysis and prediction of the possible high structure, hydrophilicity and antigenicity of YZ-2, a 22-amino acid residue partial peptide of YZ-2 was synthesized. The synthesized peptide was then used to immunize. And the properties of anti-YZ2 were analyzed by ELISA and Western blot.
  
  
  RESULTS
  The hydrophilicity and antigenicity were predicted by methods of bioinformatics. The polyclonal antibody of YZ-2 was successfully obtained and its specificity and sensitivity were conformed by ELISA and Western blot.
  
  
  CONCLUSIONS
  By the bioinformatics analysis and prediction, the hydrophilicity and antigenicity of YZ2 were analyzed. The antibody of YZ-2 was successfully obtained.
authors.affiliation: Department of Pharmacology, Pharmaceutical School, Guangdong Pharmaceutical University, Guangzhou 510006, China. zanglq@yahoo.com
authors.forename: Lin-quan
authors.initials: LQ
authors.lastname: Zang
authors.forename: Peng-xin
authors.initials: PX
authors.lastname: Qiu
authors.forename: Ru
authors.initials: R
authors.lastname: Xiao
authors.forename: Guang-mei
authors.initials: GM
authors.lastname: Yan
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D019411:Clinical Laboratory Techniques; D019295:Computational Biology; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D011506:Proteins / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000906:Antibodies; D011506:Proteins