title: Crystallization and preliminary X-ray crystallographic study of the extracellular domain of the 4-1BB ligand, a member of the TNF family.
abstract: The 4-1BB ligand, a member of the tumour necrosis factor (TNF) family, is an important co-stimulatory molecule that plays a key role in the clonal expansion and survival of CD8+ T cells. Signalling through binding of the 4-1BB ligand and 4-1BB has been reported to enhance CD8+ T-cell expansion and protect activated CD8+ T cells from death. The 4-1BB ligand is an integral protein expressed on activated antigen-presenting cells. The extracellular domain of the 4-1BB ligand fused with glutathione-S-transferase was expressed in Escherichia coli (Origami) and purified by using affinity and ion-exchange column chromatographic methods. Crystals of the 4-1BB ligand were obtained at 290 K by the hanging-drop vapour-diffusion method. X-ray diffraction data were collected from these crystals to 2.8 A resolution and the crystals belong to space group C2, with unit-cell parameters a = 114.6, b = 73.8, c = 118.50 A, beta = 115.5 degrees.
authors.affiliation: Department of Biology, Yonsei University, Seoul 120-749, South Korea.
authors.forename: Jung-Sue
authors.initials: JS
authors.lastname: Byun
authors.forename: Dong-Uk
authors.initials: DU
authors.lastname: Kim
authors.forename: Byungchan
authors.initials: B
authors.lastname: Ahn
authors.forename: Byoung Se
authors.initials: BS
authors.lastname: Kwon
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Cho
mesh_terms: D053330:4-1BB Ligand; D003460:Crystallization; D018360:Crystallography, X-Ray; D005110:Extracellular Space / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D005810:Multigene Family; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D048069:Tumor Necrosis Factors / Q000737:chemistry* / Q000235:genetics
chemical_list: D053330:4-1BB Ligand; D011993:Recombinant Fusion Proteins; C505974:TNFSF9 protein, human; D048069:Tumor Necrosis Factors