title: Enterococcus silesiacus sp. nov. and Enterococcus termitis sp. nov.
abstract: Three enterococci constituted two aberrant branches after numerical analysis of (GTG)5-PCR fingerprints: analogous patterns were found for two water isolates, strains W213 and W442T, and a separate position was found for an isolate from the gut of a termite, strain LMG 8895T. 16S rRNA gene sequence analysis classified all three strains in the Enterococcus faecalis species group. Further sequencing analysis of the housekeeping gene pheS (encoding the phenylalanyl-tRNA synthase alpha-subunit) and whole-cell-protein analysis confirmed a distinct position for the two water isolates and the termite strain, respectively. DNA-DNA hybridization experiments and distinct phenotypic features between the strains studied and representatives of the E. faecalis species group confirmed novel species status, respectively, for the two water isolates, strains W213 and W442T, and for strain LMG 8895T. The names Enterococcus silesiacus sp. nov. and Enterococcus termitis sp. nov. are proposed for the novel taxa, with W442T (= CCM 7319T = LMG 23085T) and LMG 8895T (= CCM 7300T) as the respective type strains.
authors.affiliation: Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Švec
authors.affiliation: BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Vancanneyt
authors.affiliation: Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Ivo
authors.initials: I
authors.lastname: Sedláček
authors.affiliation: Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.|BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Sabri M
authors.initials: SM
authors.lastname: Naser
authors.affiliation: BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Cindy
authors.initials: C
authors.lastname: Snauwaert
authors.affiliation: BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Karen
authors.initials: K
authors.lastname: Lefebvre
authors.affiliation: BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Hoste
authors.affiliation: Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.|BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry; D016983:Enterococcus / Q000145:classification* / Q000235:genetics / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S