Metabolic characterization of the R6/2 transgenic mouse model of Huntington's disease by high-resolution MAS 1H NMR spectroscopy.
The metabolic consequences of Huntington's disease in the R6/2 mouse model were investigated using NMR spectroscopy and pattern recognition to characterize selected brain regions, muscle, blood, and urine. Global increases in relative brain concentrations of osmolytes, creatine, glutamine, and lactate, and decreases in acetate and N-acetylaspartate were found together with striatal-specific lower concentrations of GABA and choline. Clear differentiation of R6/2 and wild-type mice was also obtained for urine and blood metabolite profiles that may have applicability for monitoring HD in human populations.
Tsang
Tsz M
TM
Biological Chemistry, Biomedical Sciences Division, Faculty of Medicine, Imperial College, London, SW7 2AZ, United Kingdom.
Woodman
Ben
B
McLoughlin
Gerard A
GA
Griffin
Julian L
JL
Tabrizi
Sarah J
SJ
Bates
Gillian P
GP
Holmes
Elaine
E
D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001923:Brain Chemistry; D004195:Disease Models, Animal; D005260:Female; D006816:Huntington Disease / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D008137:Longitudinal Studies; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D019906:Nuclear Magnetic Resonance, Biomolecular
D015415:Biomarkers