title: Interaction partners for human ZNF384/CIZ/NMP4--zyxin as a mediator for p130CAS signaling?
abstract: Transcription factor ZNF384/CIZ/NMP4 was first cloned in rat as a p130Cas-binding protein and has a role in bone metabolism and spermatogenesis. It is recurrently involved in translocations in acute lymphoblastic leukemia. Translocations t(12;17) and t(12;22) fuse ZNF384 to RNA-binding proteins TAF15 and EWSR1, while a translocation t(12;19) generates an E2A/ZNF384 fusion. We screened for ZNF384 interacting proteins using yeast two-hybrid technology. In contrast to its rat homolog, human ZNF384 does not interact with p130CAS. Zyxin, PCBP1, and vimentin, however, were identified as ZNF384-binding partners. Given the interaction between human zyxin and p130CAS, these results suggest that zyxin indirectly enables the interaction of ZNF384 with p130CAS which is described in rat.
authors.affiliation: Human Genome Laboratory, Department of Human Genetics, University of Leuven, Flanders Interuniversity Institute for Biotechnology (VIB), VIB4, Campus Gasthuisberg O&N 06, Herestraat 49 Box 602, B-3000 Leuven, Belgium.
authors.forename: Hilde
authors.initials: H
authors.lastname: Janssen
authors.forename: Peter
authors.initials: P
authors.lastname: Marynen
mesh_terms: D000818:Animals; D002460:Cell Line; D050719:Crk-Associated Substrate Protein / Q000378:metabolism*; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D006023:Glycoproteins / Q000502:physiology*; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism; D006801:Humans; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*; D035381:TATA-Binding Protein Associated Factors / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014746:Vimentin / Q000378:metabolism; D060589:Zyxin
chemical_list: C493841:BCAR1 protein, human; D050719:Crk-Associated Substrate Protein; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C094604:PCBP1 protein, human; D016601:RNA-Binding Proteins; C102041:TAF15 protein, human; D035381:TATA-Binding Protein Associated Factors; D015534:Trans-Activators; D014746:Vimentin; C498621:ZNF384 protein, human; C092842:ZYX protein, human; D060589:Zyxin