[Detection of CD95 and CD95L mRNA expression after liver transplantation using SYBR real-time PCR during acute rejection].
OBJECTIVE
To detect the expression of CD95 and CD95L mRNAs after liver transplantation and investigate the role of CD95 and CD95L in acute liver allograft rejection.


METHODS
The expressions of CD95 and CD95L mRNAs of peripheral blood lymphocyte from 56 liver allograft recipients were examined using SYBR real-time PCR.


RESULTS
CD95 and CD95L mRNA levels in the recipients with acute rejection were significantly higher than those without rejection (P<0.01), and the elevation occurred about 2 days earlier than that of alanine aminotransferase and aspartate aminotransferase.


CONCLUSIONS
CD95 and CD95L are related to acute liver allograft rejection and their mRNA expression level may serve as an indicator for prediction and diagnosis of acute rejection episodes.
Yu
Li-xin
LX
Center of Organ Transplantation, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Han
Shu-ling
SL
Miao
Yun
Y
D000208:Acute Disease; D000328:Adult; D000368:Aged; D052160:Benzothiazoles; D003959:Diamines; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006084:Graft Rejection / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008103:Liver Cirrhosis / Q000601:surgery; D008113:Liver Neoplasms / Q000601:surgery; D016031:Liver Transplantation; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D019014:fas Receptor / Q000235:genetics*
D052160:Benzothiazoles; D003959:Diamines; D053222:Fas Ligand Protein; D009930:Organic Chemicals; D011804:Quinolines; D012333:RNA, Messenger; D019014:fas Receptor; C098022:SYBR Green I