Methods for investigating placental fatty acid transport.
Fatty acids (FAs), especially essential fatty acids (EFAs) and their long chain polyunsaturated fatty acid (LCPUFAs) derivatives, are critical for proper fetal development. The fetus relies on the placental transfer of EFAs from the maternal circulation for development. In fact, fatty acid transfer is highly directional from the mother to the fetus. Significant changes in placental fatty acid transport and metabolism, the two primary processes that govern placental FA supply from mother to fetus, can subsequently result in aberrant fetal fatty acid/lipid homeostasis and dramatically increase the risk of abnormal fetal development. Besides passive diffusion, specific fatty acid transfer conferring proteins can actively mediate directional placental fatty acid uptake and transport. Enzymes for fatty acid beta-oxidation and synthesis and the ones participating PUFA metabolism, including cytochrome P450 (mainly CYP4A), cyclooxygenases (COXs), and lipooxygenases (LOXs), have also been identified in the placenta. Methods for studying functional placental fatty acid uptake/transport/metabolism are discussed, focusing on an in vitro placental trophoblast model and long chain unsaturated fatty acids. The relevant theory of FA transport pathways, kinetic data analysis (uptake rates, permeability, influx/efflux ratio, Km, and so on) and high-performance liquid chromatography identification are also discussed.
Xu
Yan
Y
Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, USA.
Cook
Thomas J
TJ
Knipp
Gregory T
GT
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D014327:Trophoblasts / Q000378:metabolism
D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids