title: Slower cycling of nestin-positive cells in neurosphere culture.
abstract: Neural stem cells are multipotent and self-renewing cells with important potential application in cell replacement therapy in brain damage. Many studies have shown that nestin-positive cells represent neural stem and progenitor cells in the central neural system. Here, we derived neural stem cells from the subventricular zone of a newborn nestin-promoter-driven green fluorescent protein mouse, and found that the percentage of nestin-positive cells decreased continuously at each passage in neurosphere culture. Using the relative proliferation ratio and relative division ratio analysis, we concluded that the slower cycling of nestin-positive cells was responsible for this decrease.
authors.affiliation: Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou, China.
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Ma
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Liu
authors.forename: Xing-Mei
authors.initials: XM
authors.lastname: Xie
authors.forename: Ai-Xia
authors.initials: AX
authors.lastname: Zhang
authors.forename: Jia-Qing
authors.initials: JQ
authors.lastname: Zhang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Yu
authors.forename: Xiu-Ming
authors.initials: XM
authors.lastname: Zhang
authors.forename: Shu-Nong
authors.initials: SN
authors.lastname: Li
authors.forename: Bruce T
authors.initials: BT
authors.lastname: Lahn
authors.forename: Andy Peng
authors.initials: AP
authors.lastname: Xiang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D018874:Spheroids, Cellular; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D049452:Green Fluorescent Proteins