title: Use of oligoarrays for characterization of community-onset methicillin-resistant Staphylococcus aureus.
abstract: Until recently, methicillin-resistant Staphylococcus aureus (MRSA) was considered the prototype of a hospital-acquired bacterial pathogen. However, recent reports have shown that MRSA has now emerged in the community. Characterization of specific markers for distinguishing the origin of isolates could contribute to improved knowledge of MRSA epidemiology. The release of whole-genome sequences of hospital- and community-acquired S. aureus strains allowed the development of whole-genome content analysis techniques, including microarrays. We developed a microarray composed of 8,191 open reading frame-specific oligonucleotides covering >99% of the four sequenced S. aureus genomes (N315, Mu50, MW2, and COL) to evaluate gene contents of hospital- and community-onset S. aureus strains. In parallel, pulsed-field gel electrophoresis, variable number of tandem repeats, antibiogram, staphylococcal cassette chromosome-mec element typing, and presence of the Panton-Valentine leukocidin gene were evaluated in a collection of 15 clinical isolates. Clusters obtained with microarrays showed a high degree of similarity with those obtained by pulsed-field gel electrophoresis or variable number of tandem repeats. Clusters clearly segregated hospital-onset strains from community-onset strains. Moreover, the microarray approach allowed definition of novel marker genes and chromosomal regions specific for given groups of isolates, thus providing better discrimination and additional information compared to pulsed-field gel electrophoresis and variable number of tandem repeats. Finally, the comparative genome hybridization approach unraveled the occurrence of multiple horizontal transfer events leading to community-onset MRSA as well as the need for a specific genetic background in recipient strains for both the acquisition and the stability of the mec element.
authors.affiliation: Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals, CH-1211 Geneva 14, Switzerland.
authors.forename: Thibaud
authors.initials: T
authors.lastname: Koessler
authors.forename: Patrice
authors.initials: P
authors.lastname: Francois
authors.forename: Yvan
authors.initials: Y
authors.lastname: Charbonnier
authors.forename: Antoine
authors.initials: A
authors.lastname: Huyghe
authors.forename: Manuela
authors.initials: M
authors.lastname: Bento
authors.forename: Sasi
authors.initials: S
authors.lastname: Dharan
authors.forename: Gesuele
authors.initials: G
authors.lastname: Renzi
authors.forename: Daniel
authors.initials: D
authors.lastname: Lew
authors.forename: Stephan
authors.initials: S
authors.lastname: Harbarth
authors.forename: Didier
authors.initials: D
authors.lastname: Pittet
authors.forename: Jacques
authors.initials: J
authors.lastname: Schrenzel
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D002648:Child; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology*; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D008297:Male; D016106:Methicillin Resistance / Q000235:genetics*; D008875:Middle Aged; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010802:Phylogeny; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D013557:Switzerland / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial