title: Expression, purification, crystallization and preliminary X-ray analysis of the human RuvB-like protein RuvBL1.
abstract: RuvBL1, an evolutionary highly conserved protein related to the AAA+ family of ATPases, has been crystallized using the hanging-drop vapour-diffusion method at 293 K. The crystals are hexagonal and belong to space group P6, with unit-cell parameters a = b = 207.1, c = 60.7 A and three molecules in the asymmetric unit.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2781-901 Oeiras, Portugal.
authors.forename: Sabine
authors.initials: S
authors.lastname: Gorynia
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Matias
authors.forename: Susana
authors.initials: S
authors.lastname: Gonçalves
authors.forename: Ricardo
authors.initials: R
authors.lastname: Coelho
authors.forename: Gonçalo
authors.initials: G
authors.lastname: Lopes
authors.forename: Mónica
authors.initials: M
authors.lastname: Thomaz
authors.forename: Martina
authors.initials: M
authors.lastname: Huber
authors.forename: Bernard
authors.initials: B
authors.lastname: Haendler
authors.forename: Peter
authors.initials: P
authors.lastname: Donner
authors.forename: Maria Arménia
authors.initials: MA
authors.lastname: Carrondo
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D017124:Conserved Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004265:DNA Helicases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D011994:Recombinant Proteins; C061260:RuvB protein, Bacteria; D000074183:ATPases Associated with Diverse Cellular Activities; D004265:DNA Helicases; C486549:RUVBL1 protein, human