title: New liquid chromatographic-chemometric approach for the determination of sunset yellow and tartrazine in commercial preparation.
abstract: A new liquid chromatographic (LC)-chemometric approach was developed for the determination of sunset yellow (SUN) and tartrazine (TAR) in commercial preparations. This approach uses LC and chemometric calibration methods, i.e., classical least-squares (CLS), principal component regression (PCR), and partial-least squares (PLS), simultaneously. The combined LC-chemometric approaches, denoted as LC-CLS, LC-PCR, and LC-PLS, are based on photodiode array (PDA) detection at multiple wavelengths. Optimum chromatographic separation of SUN and TAR with allura red as the internal standard (IS) was obtained by using a Waters Symmetry C18 column, 5 microm, 4.6 x 250 mm, and 0.2 M acetate buffer (pH 5)-acetonitrile-methano-bidistilled water (55 + 20 + 15 + 10, v/v) as the mobile phase at a flow rate of 1.9 mL/min. The LC data sets consisting of the ratios of analyte peak areas to the IS peak area were obtained by using PDA detection at 5 wavelengths (465, 470, 475, 480, and 485 nm). LC-chemometric calibrations for SUN and TAR were separately constructed by using the relationship between the peak-area ratio and the training sets for each colorant. LC-chemometric approaches were tested for different synthetic mixtures containing SUN and TAR in the presence of the IS. These LC-chemometric calibrations were applied to a commercial preparation of the 2 colorants. The experimental results of the LC-chemometric approaches were compared with those obtained by a developed classical LC method using single-wavelength detection.
authors.affiliation: Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry 06100, Tandoğan, Ankara, Turkey. dinc@pharmacy.ankara.edu.tr
authors.forename: Erdal
authors.initials: E
authors.lastname: Dinç
authors.forename: A Hakan
authors.initials: AH
authors.lastname: Aktaş
authors.forename: Ozgür
authors.initials: O
authors.lastname: Ustündağ
mesh_terms: D000465:Algorithms; D001391:Azo Compounds / Q000032:analysis*; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D016018:Least-Squares Analysis; D008027:Light; D016014:Linear Models; D008956:Models, Chemical; D004364:Pharmaceutical Preparations; D016133:Polymerase Chain Reaction; D012044:Regression Analysis; D015203:Reproducibility of Results; D013607:Tablets; D013645:Tartrazine / Q000032:analysis*; D013678:Technology, Pharmaceutical
chemical_list: D001391:Azo Compounds; D004364:Pharmaceutical Preparations; D013607:Tablets; C005842:6-hydroxy-5-((p- sulfophenyl)azo)-2-naphthalenesulfonic acid disodium salt; D013645:Tartrazine