title: Use of bovine bone graft and bone membrane in defects surgically created in the cranial vault of rabbits. Histologic comparative analysis.
abstract: OBJECTIVE
  This study was proposed to analyze histologically the process of repairing bone defects created surgically in the cranial vaults of rabbits.
  
  
  METHODS
  Thirty adult male rabbits (Oryctolagus cunilicus) received, under general anesthesia, bilateral parietal osteotomies by means of a 6-mm-diameter trephine. The bony defects were divided into 4 groups. In group 1 the defect did not receive any treatment; in group 2 the defect was filled with lyophilized bovine bone (Biograft); in group 3 it was filled with bovine bone and covered with a bone matrix membrane (Bioplate); in group 4 it was covered with a bone matrix membrane. Animals were sacrificed in 3 equal groups at 15, 30, and 60 days. The specimens were subjected to routine laboratory procedures to evaluate the degree of bone repair.
  
  
  RESULTS
  After 60 days, new bone formation in group 2 was not satisfactory when compared to that of group 3. Large amounts of new bone formation in maturation were seen in group 3. In the defects covered with a membrane the results were similar to those of group 1 (i.e., the cavity was filled with fibrous connective tissue). The implanted bone and membranes were totally resorbed.
  
  
  CONCLUSIONS
  The use of a membrane served as a barrier against the migration of cells from the adjacent tissue and the bone graft/membrane preserved the cavity space, resulting in an enhanced osteogenic effect.
authors.affiliation: Dental School at Arafatuba, UNESP, São Paulo, Brazil. thaqueiroz@hotmail.com
authors.forename: Thallita Pereira
authors.initials: TP
authors.lastname: Queiroz
authors.forename: Eduardo
authors.initials: E
authors.lastname: Hochuli-Vieira
authors.forename: Marisa Aparecida Cabrini
authors.initials: MA
authors.lastname: Gabrielli
authors.forename: Daniela Cristina Joanitti
authors.initials: DC
authors.lastname: Cancian
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D048091:Guided Tissue Regeneration / Q000379:methods*; D008297:Male; D008567:Membranes, Artificial; D011817:Rabbits; D012886:Skull / Q000601:surgery*
chemical_list: D008567:Membranes, Artificial