Distribution of the UV filter 3-benzylidene camphor in rat following topical application.
A straightforward analytical method for determination of 3-benzylidene camphor (3-BC) in rat adipose tissue, brain, liver, muscle, plasma and testis following topical application was developed and validated. Three exposure levels (60, 180 and 540 mg kg(-1) day(-1)) were tested for 65 days in male Sprague-Dawley rats (24 days postnatal). Sample preparation involving homogenization and n-heptane or methanol extraction of the tissue was applied before injection into the LC-ESI-MS-MS system. The response was linear from 2 to 100 microg l(-1) for the qualifier and the quantifier MRM transitions (R(2) (quantifier) > 0.994). Detection limit of the method corresponded to 0.005 microg g(-1) tissue and 12.5 microg l(-1) plasma, respectively. Recovery was determined for all tissues (adipose tissue: 40%; all other tissues: 80-100%) at three individual levels. 3-(4-Methyl benzylidene camphor) (4-MBC) was used throughout the study as internal standard. 3-Benzylidene camphor was detected in all tissues at all exposure levels at concentrations between 0.05 microg g(-1) (liver) and 36 microg g(-1) (adipose tissue) and in plasma at 16-89 microg l(-1). The method allowed for the quantification of 3-benzylidene camphor in all tested tissues following topical application. Furthermore, it was shown that 3-benzylidene camphor can be found in various tissues in the rat following topical application. These findings may suggest that following use of 3-benzylidene camphor containing sunscreen, similar disposition and distribution may occur in humans.
Søeborg
Tue
T
Department of Pharmaceutics and Analytical Chemistry, Danish University of Pharmaceutical Sciences, Universitetsparken 2, DK-2100 Copenhagen, Denmark. tes@dfuni.dk
Ganderup
Niels-Christian
NC
Kristensen
Jakob Højer
JH
Bjerregaard
Poul
P
Pedersen
Knud Ladegaard
KL
Bollen
Peter
P
Hansen
Steen Honoré
SH
Halling-Sørensen
Bent
B
D000287:Administration, Topical; D000818:Animals; D001593:Benzyl Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002164:Camphor / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D013473:Sunscreening Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014466:Ultraviolet Rays
D001593:Benzyl Compounds; D013473:Sunscreening Agents; C511303:3-benzylidene camphor; D002164:Camphor