[Some bone turnover markers in serum of healthy children and adolescents in relation to age and gender].
The purpose of this study was the investigation of serum concentration of serum C-telopeptide of type I collagen (s-CTX) and bone alkaline phosphatase (BALP) in Polish healthy children aged 2-18 years. We studied 141 healthy children (64 girls, 77 boys) divided into 3 age groups of both genders: prebubertal, pubertal and postpubertal. The level of s-CTX was determined by Serum CrossLaps One Step ELISA kit (Osteometer Bio Tech, Denmark) and the activity of BALP was measured using an enzyme immunoassay Alkphase-B kit (Metra Biosystems, USA). We observed that both markers of bone turnover showed sigmoid regression curves with increasing age. The peak values of s-CTX and BALP occurred during puberty in the girls aged 8-13 years (2266 +/- 368 ng/ml; 113.4 +/- 21.9 U/L) and in the boys aged 10-15 years (2139 +/- 489 ng/ml; 117.8 +/- 24.7 U/L). In all children after puberty, we observed a gradual lowering of both markers. However, girls showed decreased postpubertal values of s-CTX and BALP 2-3 years earlier than boys, reflecting the earlier completion of puberty in girls. The correlation between s-CTX and BALP was statistically significant (r = 0.578; p < 0.0001) in tested children. The results of this study will be useful in the diagnosis and monitoring of therapy in bone diseases.
Gajewska
Joanna
J
Z Zakładu Biochemii Klinicznej Instytutu Matki i Dziecka w Warszawie. biochem@imid.med.pl
Ambroszkiewicz
Jadwiga
J
Laskowska-Klita
Teresa
T
D000293:Adolescent; D000367:Age Factors; D000469:Alkaline Phosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001846:Bone Development / Q000502:physiology*; D016723:Bone Remodeling / Q000502:physiology*; D001842:Bone and Bones / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D024042:Collagen Type I / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010455:Peptides / Q000097:blood*; D011627:Puberty / Q000378:metabolism; D012016:Reference Values; D012737:Sex Factors
D015415:Biomarkers; D024042:Collagen Type I; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide; D000469:Alkaline Phosphatase