title: Recombination of Feline calicivirus within an endemically infected cat colony.
abstract: To understand the evolution of the family Caliciviridae, the persistence of Feline calicivirus (FCV) was studied within an endemically infected cat colony. Polymerase and capsid sequences were analysed for 34 FCV isolates obtained over a 4 year period. Initially, the colony was infected with one strain of virus, but a second distinct strain was later identified. Subsequently, the emergence of a recombinant virus was observed, containing elements of both of the strains circulating within the colony. The recombination event mapped close to the ORF1/ORF2 junction. This is consistent with recombination in other caliciviruses, suggesting a common mechanism within this family. This is the first report of recombination within the genus Vesivirus in the family Caliciviridae and the first time that a recombination event has been observed where the parental strains have also been identified.
authors.affiliation: Department of Veterinary Clinical Sciences, University of Liverpool Veterinary Teaching Hospital, Leahurst, Chester High Road, Neston, South Wirral CH64 7TE, UK.
authors.forename: K P
authors.initials: KP
authors.lastname: Coyne
authors.affiliation: Department of Veterinary Pathology, University of Liverpool Veterinary Teaching Hospital, Leahurst, Chester High Road, Neston, South Wirral CH64 7TE, UK.
authors.forename: F C
authors.initials: FC
authors.lastname: Reed
authors.affiliation: Department of Veterinary Pathology, University of Liverpool Veterinary Teaching Hospital, Leahurst, Chester High Road, Neston, South Wirral CH64 7TE, UK.
authors.forename: C J
authors.initials: CJ
authors.lastname: Porter
authors.affiliation: Department of Veterinary Clinical Sciences, University of Liverpool Veterinary Teaching Hospital, Leahurst, Chester High Road, Neston, South Wirral CH64 7TE, UK.
authors.forename: S
authors.initials: S
authors.lastname: Dawson
authors.affiliation: Department of Veterinary Pathology, University of Liverpool Veterinary Teaching Hospital, Leahurst, Chester High Road, Neston, South Wirral CH64 7TE, UK.
authors.forename: R M
authors.initials: RM
authors.lastname: Gaskell
authors.affiliation: Department of Veterinary Clinical Sciences, University of Liverpool Veterinary Teaching Hospital, Leahurst, Chester High Road, Neston, South Wirral CH64 7TE, UK.
authors.forename: A D
authors.initials: AD
authors.lastname: Radford
mesh_terms: D000818:Animals; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017927:Calicivirus, Feline / Q000145:classification / Q000235:genetics*; D002371:Cat Diseases / Q000453:epidemiology* / Q000821:virology; D002415:Cats; D002460:Cell Line; D019353:Endemic Diseases; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA