Identification of Spodoptera litura multicapsid nucleopolyhedrovirus ORF97, a novel protein associated with envelope of occlusion-derived virus.
Open reading frame (ORF) 97 of Spodoptera litura multicapsid nucleopolyhedrovirus (Splt97) is a member of 11k gene family (InterPro database accession number: IPR009313) in insect viruses, potentially encoding 112 amino acids with a predicted molecular weight of 13.2 kDa. Sequence analysis indicated that Splt97 has 8-50% amino acid identity with the homologues of other known baculoviruses including Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) ORF108. Splt97 transcription in SpltMNPV-infected Spli-221 cells was detected from 6 to 96 h postinfection (p.i.) and the mRNA start site was mapped within a consensus baculovirus early promoter motif (ACATT). Time course of Splt97 expression in SpltMNPV-infected cells showed that Splt97 was expressed from 48 through 96 h p.i. as a 13 kDa protein. Western blot analysis revealed that Splt97 was present in occlusion-derived virus (ODV). Furthermore, when ODV was fractionated into nucleocapsid and envelope components, Splt97 was shown to be present only in the envelope. These results suggested that Splt97 was a baculovirus ODV-associated protein.
Chen
Weichun
W
State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, People's Republic of China.
Li
Zhaofei
Z
Li
Sainan
S
Li
Lingling
L
Yang
Kai
K
Pang
Yi
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera / Q000821:virology; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
D004279:DNA, Viral; D012367:RNA, Viral; D014759:Viral Envelope Proteins