title: Intracellular IL-4, IL-10, and IFN-gamma levels of leukemic cells and bone marrow T cells in acute leukemia.
abstract: The quantitative levels of intracellular cytokines IL-4, IL-10, and IFN-gamma (ie, the number of bound PE-conjugated antibody molecules/cell) of leukemic cells and bone marrow T cells (bmT cells) of acute leukemia patients were analyzed by flow cytometry. One hundred, thirty-one (95 AML, 25 ALL, 11 ABL) patients were studied. The leukemic cell IL-4 level was highest in the monocytic AML group (1735 +/- 1056) and lowest in the dysplastic AML group (960 +/- 545). The IFN-gamma level was highest in the acute promyelocytic leukemia (APL) group (495 +/- 159), and lowest in the ALL group (252 +/- 119). The IL-10 level was not significantly different among the diagnosis groups. In bmT cells, the IL-10 level was highest in the dysplastic AML group (972 +/- 1049) and lowest in the APL group (397 +/- 352). The leukemic cell cytokine levels were lowest and bmT cell cytokine levels were highest in the dysplastic AML group. There were no significant correlations of these cytokine levels with 2-yr survival rate, complete remission (CR) rate, or relapse rate. The cytokine levels of bmT cells at the time of CR became normal and were not different among the diagnosis groups. In summary, leukemic cell and bmT cell cytoplasmic expression profiles of IL-4, IL-10, and IFN-gamma are characteristic for each diagnostic group of acute leukemia patients and the profiles of bmT cells are normal at the time of CR.
authors.affiliation: Department of Clinical Pathology, Catholic University Medical College, St. Mary's Hospital, Youngdeungpogu, Youido-dong 62, Seoul, Korea (South) 150-713.
authors.forename: Hun Hee
authors.initials: HH
authors.lastname: Park
authors.forename: Myungshin
authors.initials: M
authors.lastname: Kim
authors.forename: Bong-Hee
authors.initials: BH
authors.lastname: Lee
authors.forename: Jihyang
authors.initials: J
authors.lastname: Lim
authors.forename: Yonggoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Lee
authors.forename: Woo Sung
authors.initials: WS
authors.lastname: Min
authors.forename: Chang Suk
authors.initials: CS
authors.lastname: Kang
authors.forename: Won Il
authors.initials: WI
authors.lastname: Kim
authors.forename: Sang In
authors.initials: SI
authors.lastname: Shim
authors.forename: Kyungja
authors.initials: K
authors.lastname: Han
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood*; D015847:Interleukin-4 / Q000097:blood*; D007938:Leukemia / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D007951:Leukemia, Myeloid / Q000097:blood / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000188:drug therapy / Q000378:metabolism; D012074:Remission Induction; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma