Crystallization and preliminary crystallographic analysis of endo-1,3-beta-glucanase from Arthrobacter sp.
Endo-1,3-beta-glucanases hydrolyze internal 1,3-beta-glucosyl linkages. The endo-1,3-beta-glucanase from Arthrobacter sp. was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group P4(1), with unit-cell parameters a = 71.31, c = 60.07 A, and contained one molecule per asymmetric unit. The Matthews coefficient (VM) and the solvent content were 2.35 A3 Da(-1) and 47.63%, respectively. Diffraction data were collected to a resolution of 1.66 A at SPring-8 using a MAR CCD area detector and gave a data set with an overall Rmerge of 5.4% and a completeness of 99.4%.
Pang
Zhongcun
Z
Graduate School of Agriculture, Kyoto Prefectural University, 1-5 Shimogamo, Kyoto 606-8522, Japan.
Kang
You-Na
YN
Ban
Mizuho
M
Oda
Masayuki
M
Kobayashi
Ryo
R
Ohnishi
Masatake
M
Mikami
Bunzo
B
D001173:Arthrobacter / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D018360:Crystallography, X-Ray; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D008958:Models, Molecular; D017433:Protein Structure, Secondary
D001426:Bacterial Proteins; D006026:Glycoside Hydrolases; C028020:licheninase