title: Determination of nucleic acids based on the quenching effect on resonance light scattering of the Y(III)-1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolone-4'-)hexane-dione system.
abstract: Nucleic acids can quench resonance light scattering (RLS) intensity of the Y(III)-1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolone-4'-)hexane-dione(BPMPHD) complex in the pH range 5.0-5.8. Under optimal conditions, there are linear relationships between the quenching of RLS and the concentration of nucleic acids in the range 6.3 x 10(-8)-2.1 x 10(-5) g/mL for fish sperm DNA (fsDNA), 1.2 x 10(-8)-5.0 x 10(-5) g/mL for calf thymus DNA (ctDNA) and 6.0 x 10(-8)-2.0 x 10(-5) g/mL for yeast RNA (yRNA). The detection limits (3 s) of fsDNA, ctDNA and yRNA are 0.7 ng/mL, 3.8 ng/mL and 4.2 ng/mL, respectively.
authors.affiliation: Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Jing He
authors.initials: JH
authors.lastname: Yang
authors.forename: Shuna
authors.initials: S
authors.lastname: Sun
authors.forename: Changying
authors.initials: C
authors.lastname: Guo
authors.forename: Dehuan
authors.initials: D
authors.lastname: Ran
authors.forename: Jinhua
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000737:chemistry; D005399:Fishes; D006863:Hydrogen-Ion Concentration; D008297:Male; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry*; D011720:Pyrazoles / Q000737:chemistry*; D012313:RNA / Q000032:analysis / Q000737:chemistry; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013094:Spermatozoa / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism
chemical_list: C512861:1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolon-4'-)hexanedione; D000447:Aldehydes; D009696:Nucleic Acids; D011720:Pyrazoles; D012313:RNA; D004247:DNA; C427203:calf thymus DNA