Oxidation-reduction respiratory chains and ATP synthase complex are localized in detergent-resistant lipid rafts.
In order to detect and identify ubiquitous lipid raft marker proteins, we isolated lipid rafts from different mouse organs, including the liver, lung, large brain, and kidney, and analyzed their proteins via 2-DE. Many protein spots were determined to be ubiquitous in all of the lipid rafts, and were annotated via LC and MS/MS. Twelve proteins were identified as ubiquitous raft proteins, and most of these were determined to be mitochondrial proteins, including mortalin, prohibitin, voltage-dependent anion channel, ATP synthase, NADH dehydrogenase, and ubiquinol-cytochrome c reductase. Via immunoblotting, these proteins were shown to exist in detergent-resistant lipid rafts prepared using different organ tissues. Since these oxidation-reduction respiratory chains and ATP synthase complex were detected in detergent-resistant lipid raft fractions which had been isolated from the plasma membrane but not from the mitochondria, and found in the cell surface when determined by immunofluoresence and immunohistochemistry, we conclude that plasma membrane lipid rafts might contain oxidation-reduction respiratory chains and ATP synthase complex.
Kim
Ki-Bum
KB
School of Life Sciences and Biotechnology, Korea University, Anam-Dong, Sungbook-Ku, Seoul, Korea.
Lee
Joong-Won
JW
Lee
Chang Seok
CS
Kim
Bong-Woo
BW
Choo
Hyo-Jung
HJ
Jung
Soon-Young
SY
Chi
Sung-Gil
SG
Yoon
Young-Sil
YS
Yoon
Gyesoon
G
Ko
Young-Gyu
YG
D025181:ATP Synthetase Complexes / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002853:Chromatography, Liquid / Q000379:methods; D003902:Detergents / Q000494:pharmacology*; D004579:Electron Transport; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D013058:Mass Spectrometry / Q000379:methods; D021962:Membrane Microdomains / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D040901:Proteomics / Q000379:methods*
D003902:Detergents; D025181:ATP Synthetase Complexes