title: [Isolation of a novel peptide that binds to BLyS from a 12-mer phage display peptide library].
abstract: A 12-mer phage display peptide library was screened for specific binders against B lymphocyte stimulator (BLyS). After 3 rounds of panning, positive phage clones were enriched. ELISAs were used to identify positive phages, and the DNA encoding the positive peptide (RHKIQLRQNIIT) was cloned and expressed as a GST fusion protein in E. coli. After purification, the identity of the fusion protein was confirmed through its specific binding to BLyS by ELISA. We have obtained a peptide that can bind to BLyS and probably act as an antagonistic peptide against the natural BLyS receptor.
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. dingylding@yahoo.com.cn
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Ding
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Qiong
authors.initials: Q
authors.lastname: Shen
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Gong
mesh_terms: D053264:B-Cell Activating Factor / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D053264:B-Cell Activating Factor; D019151:Peptide Library; D010455:Peptides