title: Ex vivo biochemical analysis of CFTR in human rectal biopsies.
abstract: This report describes the first biosynthetic analysis of the cystic fibrosis transmembrane conductance regulator (CFTR) in freshly excised human rectal biopsies. Expression of functional CFTR was assessed by intestinal current measurement (ICM) prior to biosynthetic studies. Several structural features of CFTR are found to be comparable to those established in CFTR-expressing cell lines. Interestingly, maturation of CFTR increases substantially in tissue incubated at 26 degrees C. Our data provide a solid basis for future studies on the characterisation of CFTR in pathological cases.
authors.affiliation: Klinische Forschergruppe, OE 6711, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, D-30623 Hannover, Germany. barneveld.andrea.van@mh-hannover.de
authors.forename: Andrea
authors.initials: A
authors.lastname: van Barneveld
authors.forename: Frauke
authors.initials: F
authors.lastname: Stanke
authors.forename: Manfred
authors.initials: M
authors.lastname: Ballmann
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Naim
authors.forename: Burkhard
authors.initials: B
authors.lastname: Tümmler
mesh_terms: D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002217:Carbachol / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D005576:Colforsin / Q000494:pharmacology; D003545:Cysteine / Q000378:metabolism; D003550:Cystic Fibrosis / Q000473:pathology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D004594:Electrophysiology; D006632:Histamine / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D047468:Immunoprecipitation; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000737:chemistry / Q000473:pathology; D007553:Isotope Labeling; D008875:Middle Aged; D012007:Rectum / Q000737:chemistry* / Q000473:pathology; D013462:Sulfur Radioisotopes
chemical_list: D013462:Sulfur Radioisotopes; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005576:Colforsin; D006632:Histamine; D002217:Carbachol; D003545:Cysteine