title: Expression of chemokine-like factor 1 is upregulated during T lymphocyte activation.
abstract: Chemokine-like factor 1 (CKLF1) is a cytokine with chemotactic effects on leukocytes and a functional ligand of CCR4. This cytokine is widely expressed and the level of expression is reported to be upregulated in asthma and rheumatoid arthritis (RA), disease conditions in which T lymphocytes are over-activated. In order to determine the expression profile of CKLF1 in activated T lymphocytes, we first employed a PCR-based method on human blood fractions cDNA panels and found that CKLF1 was upregulated in activated CD4+ and CD8+ cells, with no obvious changes in CD19+ cells. We further performed kinetic analyses of CKLF1 expression in phytohemagglutinin (PHA)-stimulated human peripheral blood lymphocytes (PBL) at both the mRNA and protein levels. In resting PBL, the constitutive expression of CKLF1 was low at mRNA level and barely detectable at the protein level; however, both were remarkably upregulated by PHA, appearing at 8h after PHA-stimulation and persisting up to 72h. These results suggest that CKLF1 may be involved in T lymphocyte activation and further study of CKLF1 function will prove valuable.
authors.affiliation: Lab of Medical Immunology, School of Basic Medical Science, Peking University, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Ji
authors.initials: J
authors.lastname: Zhong
authors.forename: Yingyu
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qiu
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Dalong
authors.initials: D
authors.lastname: Ma
authors.forename: Wenling
authors.initials: W
authors.lastname: Han
mesh_terms: D018941:Antigens, CD19 / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D002478:Cells, Cultured; D018925:Chemokines / Q000096:biosynthesis*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology*; D062792:MARVEL Domain-Containing Proteins; D010835:Phytohemagglutinins / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D018941:Antigens, CD19; C432676:CKLF protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2