In vitro sulfation of N-acetyllactosaminide by soluble recombinant human beta-Gal-3'-sulfotransferase.
Membrane-bound beta-Gal-3'-sulfotransferase (GP3ST) was expressed and used for in vitro sulfation of Tamm-Horsfall glycoprotein. Further, the regioselective transfer of sulfate to an N-acetyllactosamine derivative could be realised with soluble chimeric GP3ST, also in combination with Lac transglycosylation by means of beta-galactosidase. Two alternative straightforward chemical syntheses for the target compound could be elaborated.
Greimel
P
P
Institute of Organic Chemistry, University of Hamburg, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany.
Jabs
Sabrina
S
Storch
Stefan
S
Cherif
Slim
S
Honke
Koichi
K
Braulke
Thomas
T
Thiem
Joachim
J
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D006224:Cricetinae; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D009088:Mucoproteins / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D013205:Staphylococcal Protein A / Q000737:chemistry; D013431:Sulfates / Q000378:metabolism; D015238:Sulfotransferases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000652:urine; D058949:Uromodulin
D009088:Mucoproteins; D009844:Oligosaccharides; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; D013431:Sulfates; C548612:UMOD protein, human; D058949:Uromodulin; D015238:Sulfotransferases; C466142:glycoprotein-3-sulfotransferase; D006026:Glycoside Hydrolases