Use of Sno Strip filter-paper wicks for collection of genital-tract samples allows reproducible determination of human immunodeficiency virus type 1 (HIV-1) RNA viral load with a commercial HIV-1 viral load assay.
To assess the reproducibility of measurements of cervical and vaginal human immunodeficiency virus (HIV) viral load, 92 duplicate cervical and 88 duplicate vaginal samples were collected from 13 HIV-infected women using Sno Strip filter-paper wicks. RNA was eluted from the strips, extracted, and assayed using a modified protocol for the Roche Cobas Amplicor HIV-1 Monitor assay. Pearson's correlation coefficient (R), coefficient of determination (D), and Bland-Altman plots (BA) were used to compare paired log10-transformed viral loads. Analysis of duplicate same-site samples showed good reproducibility (cervix: R = 0.72, D = 52%, BA = 89% within range; vagina: R = 0.72, D = 51%, BA = 87% within range); paired cervix/vagina measurements showed moderate correlation only (R = 0.56; D = 31.3%). Standardized sample collection and simple modification of the Roche Cobas Amplicor HIV-1 Monitor assay allows reproducible measurement of genital viral load.
Sherlock
Christopher H
CH
Diagnostic Virology and Reference Laboratory, Providence Health Care, University of British Columbia, 4500 Oak Street, Vancouver, BC V6H 3N1, Canada. csherloc@interchange.ubc.ca
Lott
Paula M
PM
Money
Deborah M
DM
Merrick
Linda
L
Arikan
Yasemin
Y
Remple
Valencia P
VP
Craib
Kevin
K
Burdge
David R
DR
D002584:Cervix Uteri / Q000821:virology; D005260:Female; D005836:Genitalia, Female / Q000821:virology*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D015203:Reproducibility of Results; D014599:Uterus / Q000821:virology; D014773:Virology / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data
D012367:RNA, Viral