title: Determination of Fc function with frozen red blood cells.
abstract: The Fc function of immunoglobulins is commonly determined by an assay based on monitoring immunoglobulin induced, complement mediated red cell lysis. This assay requires a continuous source of fresh red cells. We have shown that the assay can be successfully performed with frozen red cells. The possibility of access to a stored standard stock of red cells will improve the convenience of performing the assay and could contribute to improved assay reproducibility.
authors.affiliation: CSL Bioplasma, Research and Development, 189-209 Camp Road, Broadmeadows, Victoria 3047, Australia.
authors.forename: V
authors.initials: V
authors.lastname: Gurevich
authors.forename: J
authors.initials: J
authors.lastname: Bertolini
authors.forename: K
authors.initials: K
authors.lastname: Lyons
mesh_terms: D001681:Biological Assay; D001793:Blood Preservation; D015925:Cryopreservation; D004912:Erythrocytes / Q000187:drug effects*; D005615:Freezing; D006461:Hemolysis; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology / Q000494:pharmacology*
chemical_list: D007141:Immunoglobulin Fc Fragments