Crystallization and preliminary crystallographic analysis of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica.
Crystals of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica (molecular weight 36.5 kDa) have been grown at 291 K using 2.5 M NaCl as precipitant. The crystals diffract to 3.0 A resolution at 100 K. The crystals belong to space group P321, with unit-cell parameters a = 121.98, b = 121.98, c = 56.71 A. There is one molecule in the asymmetric unit and the solvent content is estimated to be 62.1% by volume.
Kachalova
Galina S
GS
Max-Planck Unit for Structural Molecular Biology, Protein Dynamics Group, Hamburg 22607, Germany. galina@mpghdb.desy.de
Artyukh
Rimma I
RI
Lavrova
Natalia V
NV
Ryazanova
Elena M
EM
Karyagina
Anna S
AS
Kubareva
Elena A
EA
Bartunik
Hans D
HD
D003460:Crystallization; D018360:Crystallography, X-Ray; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000737:chemistry*; D042806:Neisseria lactamica / Q000201:enzymology*
D004248:DNA (Cytosine-5-)-Methyltransferases