title: Human papillomavirus infections in women seeking cervical Papanicolaou cytology of Durango, Mexico: prevalence and genotypes.
abstract: BACKGROUND
  HPV infection in women from developing countries is an important public health problem. Therefore, we sought to determine the prevalences of HPV infection and HPV genotypes in a female population of Durango City, Mexico. Also to determine whether any socio-demographic characteristic from the women associated with HPV infection exists.
  
  
  METHODS
  Four hundred and ninety eight women seeking cervical Papanicolaou examination in three public Health Centers were examined for HPV infection. All women were tested for HPV DNA PCR by using HPV universal primers. In addition, all positive HPV DNA PCR samples were further analyzed for genotyping of HPV genotype 16, 18 and 33. Socio-demographic characteristics from each participant were also obtained.
  
  
  RESULTS
  Twenty-four out of four hundred and ninety-eight (4.8%) women were found infected by HPV. HPV genotype 16 was found in 18 out of the 24 (75%) infected women. Two of them were also coinfected by HPV genotype 18 (8.3%). In the rest 6 PCR positive women, genotyping for HPV genotypes 16, 18 and 33 were negative.
  
  
  CONCLUSIONS
  The prevalence of HPV in women of Durango City is low; however, most infected women have high risk HPV genotype. The women who were studied showed low frequency of risk factors for HPV infection and this may explain the low prevalence of HPV infection. The high frequency of high risk HPV genotypes observed might explain the high rate of mortality for cervical cancer in our region.
authors.affiliation: Facultad de Medicina, Universidad Juárez del Estado de Durango, Durango, Mexico. lfsanguiano@hotmail.com
authors.forename: Luis Francisco
authors.initials: LF
authors.lastname: Sánchez-Anguiano
authors.forename: Cosme
authors.initials: C
authors.lastname: Alvarado-Esquivel
authors.forename: Miguel Arturo
authors.initials: MA
authors.lastname: Reyes-Romero
authors.forename: Margarita
authors.initials: M
authors.lastname: Carrera-Rodríguez
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005838:Genotype; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D015995:Prevalence; D014626:Vaginal Smears