Amarogentin can reduce hyperproliferation by downregulation of Cox-II and upregulation of apoptosis in mouse skin carcinogenesis model.
Swertia chirata, is a bitter plant, used in the Indian system of medicine (Ayurveda) for various human ailments. Our laboratory was the first to report the chemopreventive effect of this plant. The antiproliferative and pro-apoptotic action of amarogentin rich fraction of S. chirata is now demonstrated on a mouse skin carcinogenesis model. Immunohistochemical localization revealed a reduction in proliferating and increase in apoptotic cells in skin lesion following treatment, also reflected in the expression of molecular markers--Cox-II and caspase-3 proteins. It may be possible to calculate relative risk, relative protection and attributable risk from the action of test agents on proliferation and apoptosis.
Saha
Prosenjit
P
Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, Kolkata, India. prosenjit_cnci@yahoo.co.in
Mandal
Suvra
S
Das
Ashes
A
Das
Sukta
S
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D004195:Disease Models, Animal; D015536:Down-Regulation; D004591:Electrophoresis, Polyacrylamide Gel; D005960:Glucosides / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D039823:Iridoids / Q000494:pharmacology*; D008297:Male; D008519:Medicine, Traditional; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D012878:Skin Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D029967:Swertia; D015854:Up-Regulation
D005960:Glucosides; D039823:Iridoids; D008565:Membrane Proteins; D010936:Plant Extracts; D015127:9,10-Dimethyl-1,2-benzanthracene; C102609:amarogentin; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D053148:Caspase 3