[Time-dependent expression of astroglial S100beta following diffuse brain injury in rats].
OBJECTIVE
To investigate the dynamics of the induction of S100beta in different parts of rat brain following the diffuse brain injury.


METHODS
Immunohistochemistry and auto-image analysis were to determine the expression of astroglial S100beta after diffuse brain injury in rats. Forty rats were distributed into groups according to injury time of 30min, and2,4,12,24h, and 3,6 d after diffuse brain injury, and normal rats as control.


RESULTS
The number of S100beta positive cells in the four areas increased significantly followed by a decrease, and then a further increase. The expression of S100beta could be detected increasing in 2h, and increased significantly in 4h, and it reached apex 12h after DBI, and decreased gradually to the level less than normal 3d, and returned to normal 7d following injury. In the postmortem injury groups, there were no significant changes in anti-S100beta immunoreactivities in four areas of brain compared to the control group.


CONCLUSIONS
The present study showed the time-dependent expression of S100beta is obvious following diffuse brain injury, and suggested S100beta be suitable as a marker for brain injury age determination.
Huang
Ping
P
Key Laboratory of environment and Genes Related to Diseases of Ministry of Education, Xi'an Jiaotong University, Xi'an 710061, China.
Liu
Yan-feng
YF
Tuo
Ya
Y
Zhang
Ping
P
Ding
Cun-jing
CJ
Fang
Jie
J
Wang
Zheng-yuan
ZY
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001929:Brain Edema / Q000209:etiology / Q000473:pathology; D001930:Brain Injuries / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009414:Nerve Growth Factors / Q000032:analysis*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000032:analysis*; D013194:Staining and Labeling; D013997:Time Factors
D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins