title: RT-PCR analysis of Candida albicans ALS gene expression in a hyposalivatory rat model of oral candidiasis and in HIV-positive human patients.
abstract: ALS gene expression was studied in the hyposalivatory rat model of oral candidiasis and in clinical specimens collected from HIV-positive patients to assess similarities in expression patterns between the model system and clinical isolates. Two Candida albicans strains, SC5314 and OY-2-76, were used in the rat model system and infection progressed for 3 or 5 days. The strains produced similar oral lesions at 3 days. At 5 days, strain OY-2-76 produced more superficial lesions containing relatively more yeast forms compared to invasive hyphal forms observed for strain SC5314. For all infections, the most severe lesions were observed on the tongue and gingiva overlying the mandible. ALS transcripts were easier to detect by RT-PCR later in infection and under other conditions where more fungal cells were present. Expression of ALS1, ALS2, ALS3 and ALS4 was observed in rats infected for 3 days with ALS5 and ALS9 transcripts detected after 5 days of infection. Expression of ALS6 was observed in a single specimen from a 5-day infection while ALS7 transcript was never found. Expression of all ALS genes was observed in oral clinical material collected from HIV-positive patients although ALS6 and ALS7 transcripts required an extra PCR amplification step to be detected. Overall, the patterns of ALS gene expression were similar between the rat model and human clinical specimens, suggesting that the model would be useful for studying the phenotype of al delta/al delta mutant strains.
authors.affiliation: Department of Pathobiology, University of Illinois, Urbana, Illinois 61802, USA.
authors.forename: Clayton B
authors.initials: CB
authors.lastname: Green
authors.forename: Sandra Manfra
authors.initials: SM
authors.lastname: Marretta
authors.forename: Georgina
authors.initials: G
authors.lastname: Cheng
authors.forename: Fady F
authors.initials: FF
authors.lastname: Faddoul
authors.forename: E J
authors.initials: EJ
authors.lastname: Ehrhart
authors.forename: Lois L
authors.initials: LL
authors.lastname: Hoyer
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology* / Q000473:pathology; D000328:Adult; D000818:Animals; D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism; D002180:Candidiasis, Oral / Q000382:microbiology* / Q000473:pathology / Q000821:virology; D004195:Disease Models, Animal; D005260:Female; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D006678:HIV / Q000254:growth & development; D015658:HIV Infections / Q000382:microbiology* / Q000821:virology; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000382:microbiology; D012313:RNA / Q000737:chemistry / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014987:Xerostomia / Q000382:microbiology*
chemical_list: D005656:Fungal Proteins; D012313:RNA