Germ line specific expression of a protein phosphatase Y interacting protein (PPYR1) in Drosophila.
PPYR1, the product of the CG15031 gene, was identified as a protein phosphatase Y (PPY) interacting protein in Drosophila melanogaster using a yeast two-hybrid screen. PPYR1 displays a biphasic expression pattern: the maternal protein is abundant in the developing egg chambers and in the early embryos, while the zygotic protein appears later in development and is localized specifically in the testes of the males. The maternal and zygotic gene products differ from each other in their size having apparent molecular masses of 47 and 66 kDa, respectively. The maternal PPYR1 is localized in the cytoplasm of the follicular and nurse cells and is deposited as a ribonucleoprotein complex in the oocyte. In the early embryos, the PPYR1 is distributed evenly, and it gradually diminishes during embryonic development. Zygotic PPYR1 is expressed exclusively in the testes, predominantly in the cytoplasm of the spermatocytes. PPY is localized in the nuclei of the same cells. Our results suggest that PPYR1 has two distinct developmental isoforms: a maternal protein the expression of which is independent of PPY and a zygotic protein which is co-expressed with PPY.
Kókai
Endre
E
Department of Medical Chemistry, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Hungary.
Szuperák
Milán
M
Alphey
Luke
L
Gausz
János
J
Adám
Géza
G
Dombrádi
Viktor
V
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D009865:Oocytes / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D020033:Protein Isoforms; D012333:RNA, Messenger; D013090:Spermatocytes / Q000378:metabolism; D013737:Testis / Q000196:embryology / Q000378:metabolism
D029721:Drosophila Proteins; C512449:PPYR1 protein, Drosophila; D020033:Protein Isoforms; D012333:RNA, Messenger; C104054:PpY-55A protein, Drosophila; D010749:Phosphoprotein Phosphatases