Human splenic dendritic cells react differently to bacterial and allogeneic antigens.
We investigated the ability of bacterial (cells, LPS, and DNA) or allogeneic antigens to stimulate splenic dendritic cells (DCs) and expression of Toll-like receptors (TLRs), CD14 (co-functional molecule to TLR), CD83 (activation molecule on migrating DCs), CD123 (IL-3R specific for myeloid DC), Hsp60 and Hsp90 (heat shock proteins) involved in recognizing pathogen-associated molecular patterns (PAMP) and other pathogen recognition receptors (PRR) ligands. Allogeneic stimulation of DC TLRs was weak compared with that of bacterial products. This suggests that the highly conserved TLRs destined to react to bacterial products do not recognize donor products differing at the major histocompatibility complex (MHC) locus, at least using in vitro culture conditions.
Stanislawska
J
J
Department of Surgical Research and Transplantation, Medical Research Center, Polish Academy of Sciences, ul. Pawinskiego 5, 02-106 Warsaw, Poland.
Interewicz
B
B
Durlik
M
M
Olszewski
W L
WL
D000942:Antigens, Bacterial / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D007519:Isoantigens / Q000276:immunology*; D013154:Spleen / Q000276:immunology*
D000942:Antigens, Bacterial; D015703:Antigens, CD; D007519:Isoantigens