title: Crystallization and preliminary X-ray diffraction analyses of pseudechetoxin and pseudecin, two snake-venom cysteine-rich secretory proteins that target cyclic nucleotide-gated ion channels.
abstract: Cyclic nucleotide-gated (CNG) ion channels play pivotal roles in sensory transduction of retinal and olfactory neurons. The elapid snake toxins pseudechetoxin (PsTx) and pseudecin (Pdc) are the only known protein blockers of CNG channels. These toxins are structurally classified as cysteine-rich secretory proteins and exhibit structural features that are quite distinct from those of other known small peptidic channel blockers. This article describes the crystallization and preliminary X-ray diffraction analyses of these toxins. Crystals of PsTx belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 60.30, b = 61.59, c = 251.69 A, and diffraction data were collected to 2.25 A resolution. Crystals of Pdc also belonged to space group P2(1)2(1)2(1), with similar unit-cell parameters a = 60.71, b = 61.67, c = 251.22 A, and diffraction data were collected to 1.90 A resolution.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Suzuki
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Zui
authors.initials: Z
authors.lastname: Fujimoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Morita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuno
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D054815:Cyclic Nucleotide-Gated Cation Channels; D003545:Cysteine / Q000032:analysis*; D004546:Elapid Venoms / Q000737:chemistry*; D007473:Ion Channels / Q000378:metabolism*; D012910:Snake Venoms / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D054815:Cyclic Nucleotide-Gated Cation Channels; D004546:Elapid Venoms; D007473:Ion Channels; D012910:Snake Venoms; C117676:pseudechetoxin, Pseudechis australis; D003545:Cysteine