Novel magnetic and fluorescent nanocomposite as a sensitive probe for the determination of proteins.
The Fe(3)O(4)/(sodium oleic acid/ethyltrimethyl ammonium bromide)(n)/4-aminobenzoic acid (Fe(3)O(4)/(NaOL/CTAB)(n)/PABA) nanocomposites have been prepared by a layer-by-layer self-assembly approach. This kind of nanocomposites have fluorescent, magnetic and water-soluble properties. Taking advantage of the magnetic property of nanocomposites, we can separated them from solution easily by using a permanent magnet. By using their strong fluorescence, we can detect proteins. At pH 6.98, the fluorescence of Fe(3)O(4)/(NaOL/CTAB)(n)/PABA nanocomposites can be enhanced by the proteins. Under optimal conditions, the linear ranges of calibration curves were 0.2-20, 0.2-13, 0.2-10 microg mL(-1) for gamma-globulin (gamma-IgG), human serum albumin (HSA), and bovine serum albumin (BSA), respectively. The detection limits were 0.02, 0.01, 0.02 for gamma-IgG, HSA and BSA, respectively. The method has been applied to analyze the total proteins in human samples and the results were in good agreement with those reported by the hospital. This method is sensitive, simple and potential in many areas.
Wang
Lun
L
College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China. WangLun@mail.ahnu.edu.cn
Hong
Shi
S
Wang
Leyu
L
Dong
Li
L
Bian
Guirong
G
Xia
Tingting
T
Chen
Hongqi
H
D010129:4-Aminobenzoic Acid / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D002138:Calibration; D002417:Cattle; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D058085:Iron Compounds / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry; D008280:Magnetics; D008854:Microscopy, Electron; D053761:Nanocomposites / Q000032:analysis* / Q000737:chemistry / Q000648:ultrastructure; D009994:Osmolar Concentration; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014867:Water; D005719:gamma-Globulins / Q000032:analysis / Q000737:chemistry
D005456:Fluorescent Dyes; D058085:Iron Compounds; D011506:Proteins; D012996:Solutions; D005719:gamma-Globulins; D014867:Water; D012710:Serum Albumin, Bovine; D010129:4-Aminobenzoic Acid