Misacylation of tRNA in prokaryotes: a re-evaluation.
Misacylation of tRNA by a non-cognate amino acid is a natural phenomenon and occurs with a frequency of approximately 1 in 10,000 due to occasional mistakes in aminoacyl transfer RNA (tRNA) synthesis. In a number of prokaryotic organisms, misacylation of selenocysteinyl tRNA, glutaminyl tRNA and aspartyl tRNAs has particular physiological meaning. Recently, misacylation has emerged as a powerful tool for studying specific interactions between aa-tRNAs and associated protein factors. The present review provides an overview of the application of misacylated tRNA in research.
Stortchevoi
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School of Medicine, Yale University, New Haven, Connecticut, 06510, USA. alexei.stortchevoi@yale.edu
D000215:Acylation; D009154:Mutation; D011387:Prokaryotic Cells / Q000502:physiology*; D011506:Proteins / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics* / Q000378:metabolism*
D011506:Proteins; D012343:RNA, Transfer