The cloning and characterization of a soluble epoxide hydrolase in chicken.
The mammalian soluble epoxide hydrolase (sEH) plays a role in the regulation of blood pressure and vascular homeostasis through its hydrolysis of the endothelial-derived messenger molecules, the epoxyeicosatrienoic acids. This study reports the cloning and expression of a sEH homolog from chicken liver. The resulting 63-kDa protein has an isoelectric point of 6.1. The recombinant enzyme displayed epoxide hydrolase activity when assayed with [3H]-trans-1,3-diphenylpropene oxide (t-DPPO), as well as trans-9,10-epoxystearate and the cis-8,9-, 11,12-, and 14,15- epoxyeicosatrienoic acids. The chicken enzyme displayed a lower kcat:Km for t-DPPO than the mammalian enzymes. The enzyme was sensitive to urea-based inhibitors developed for mammalian sEH. Such compounds could be used to study the role of chicken sEH in conditions in which endothelial-derived vasodilation is believed to be impaired, such as pulmonary hypertension syndrome.
Harris
T R
TR
Department of Entomology and Cancer Research Center, University of California, Davis 95616, USA.
Morisseau
C
C
Walzem
R L
RL
Ma
S J
SJ
Hammock
B D
BD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D004730:Endothelium, Vascular / Q000502:physiology; D004851:Epoxide Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004852:Epoxy Compounds / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D011994:Recombinant Proteins; D016415:Sequence Alignment; D012995:Solubility; D013379:Substrate Specificity; D014316:Tritium; D014664:Vasodilation
C096857:1,3-diphenylpropene oxide; D018076:DNA, Complementary; D004852:Epoxy Compounds; D011994:Recombinant Proteins; D014316:Tritium; D004851:Epoxide Hydrolases