Crystallization and preliminary X-ray analysis of the YjgF/YER057c/UK114-family protein ST0811 from Sulfolobus tokodaii strain 7.
ST0811 from Sulfolobus tokodaii strain 7, a member of the YjgF/YER057c/UK114 protein family, was crystallized by the sitting-drop vapour-diffusion method using PEG 10,000 as precipitant. The crystals diffracted X-rays to beyond 2.0 A resolution using an in-house rotating-anode generator. The crystals belonged to the rhombohedral space group R3, with hexagonal unit-cell parameters a = b = 55.0, c = 223.2 A. The crystals contained two molecules in the asymmetric unit (VM = 2.3 A3 Da(-1)) and had a solvent content of 47%.
Miyakawa
Takuya
T
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan. amtanok@mail.ecc.u-tokyo.ac.jp
Hatano
Ken Ichi
KI
Lee
Woo Cheol
WC
Kato
Yusuke
Y
Sawano
Yoriko
Y
Yumoto
Fumiaki
F
Nagata
Koji
K
Tanokura
Masaru
M
D019843:Archaeal Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D016944:Sulfolobus / Q000737:chemistry* / Q000145:classification*
D019843:Archaeal Proteins