title: Atrial natriuretic peptide enhances cortisol secretion from guinea-pig adrenal gland: evidence for an indirect paracrine mechanism probably involving the local release of medullary catecholamines.
abstract: Atrial natriuretic peptide (ANP) is a regulatory hormone widely expressed, along with its receptors, in organs and body tissues. ANP is well known to inhibit aldosterone secretion from mammalian adrenals, but its effect on glucocorticoid-hormone production is controversial. In vivo experiments showed that prolonged ANP administration raised the plasma concentration of cortisol in both normal and dexamethasone/captopril-treated guinea pigs (i.e. in animals with pharmacologically interrupted hypothalamic-pituitary-adrenal axis and renin-angiotensin system). ANP did not affect cortisol secretion from dispersed guinea pig zona fasciculata-reticularis cells, but enhanced catecholamine release from adrenomedullary cells. ANP stimulated cortisol output from guinea pig adrenal slices containing medullary chromaffin tissue, and the beta-adrenoceptor antagonist l-alprenolol blocked this effect. The conclusion is drawn that ANP, when the structural integrity of the adrenal gland is preserved, is able to enhance glucocorticoid secretion in guinea pigs, through an indirect mechanism involving the rise in the catecholamine release, which in turn, acting in a paracrine manner, stimulate secretion of inner adrenocortical cells.
authors.affiliation: Department of Zoology, Ranchi University, Ranchi 834008, India.
authors.forename: Dipali
authors.initials: D
authors.lastname: Raha
authors.forename: Cinzia
authors.initials: C
authors.lastname: Tortorella
authors.forename: Giuliano
authors.initials: G
authors.lastname: Neri
authors.forename: Anita
authors.initials: A
authors.lastname: Prasad
authors.forename: Bushra
authors.initials: B
authors.lastname: Raza
authors.forename: Ranu
authors.initials: R
authors.lastname: Raskar
authors.forename: Rakhi
authors.initials: R
authors.lastname: Dubey
authors.forename: Nisarga S
authors.initials: NS
authors.lastname: Sen
authors.forename: Carlo
authors.initials: C
authors.lastname: Macchi
authors.forename: Ludwick K
authors.initials: LK
authors.lastname: Malendowicz
authors.forename: M Firoz
authors.initials: MF
authors.lastname: Ahmad
authors.forename: Gastone G
authors.initials: GG
authors.lastname: Nussdorfer
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects* / Q000378:metabolism; D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism; D000526:Alprenolol / Q000008:administration & dosage / Q000494:pharmacology; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000008:administration & dosage / Q000494:pharmacology*; D002216:Captopril / Q000008:administration & dosage / Q000494:pharmacology; D002395:Catecholamines / Q000378:metabolism; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000378:metabolism; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage / Q000494:pharmacology; D006168:Guinea Pigs; D006854:Hydrocortisone / Q000097:blood / Q000378:metabolism*; D066298:In Vitro Techniques; D007279:Injections, Subcutaneous; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D019899:Paracrine Communication / Q000187:drug effects / Q000502:physiology
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D002395:Catecholamines; D005938:Glucocorticoids; D003907:Dexamethasone; D009320:Atrial Natriuretic Factor; D000526:Alprenolol; D002216:Captopril; D006854:Hydrocortisone; D009638:Norepinephrine; D004837:Epinephrine