Structure at 1.6 A resolution of the protein from gene locus At3g22680 from Arabidopsis thaliana.
The gene product of At3g22680 from Arabidopsis thaliana codes for a protein of unknown function. The crystal structure of the At3g22680 gene product was determined by multiple-wavelength anomalous diffraction and refined to an R factor of 16.0% (Rfree = 18.4%) at 1.60 A resolution. The refined structure shows one monomer in the asymmetric unit, with one molecule of the non-denaturing detergent CHAPS {3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate} tightly bound. Protein At3g22680 shows no structural homology to any other known proteins and represents a new fold in protein conformation space.
Allard
Simon T M
ST
Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin-Madison, USA.
Bingman
Craig A
CA
Johnson
Kenneth A
KA
Wesenberg
Gary E
GE
Bitto
Eduard
E
Jeon
Won Bae
WB
Phillips
George N
GN
D017360:Arabidopsis / Q000378:metabolism*; D002793:Cholic Acids / Q000494:pharmacology; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D015233:Models, Statistical; D011487:Protein Conformation; D012645:Selenomethionine / Q000737:chemistry; D048372:Streptomyces coelicolor / Q000378:metabolism; D014961:X-Ray Diffraction
D002793:Cholic Acids; D003902:Detergents; D012645:Selenomethionine; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate