Absence of MutY homologue mutation in patients with multiple sporadic adenomatous polyps in Korea.
OBJECTIVE
Recently, germ-line mutation in the base excision repair gene MYH has been identified to cause a novel autosomal recessive form of familial adenomatous polyposis (FAP). Interestingly, a striking evidence for MYH mutations within different ethnic groups has been demonstrated. In this study, we screened 30 patients with multiple adenomatous polyps for MYH mutations to assess its prevalence and ethnic specificity in Korea.


METHODS
Thirty patients (21 men and 9 women; mean age 62.3 years) with multiple adenomatous polyps were examined for MYH mutations. The mean number of adenomas per patient was 10.0. Sixteen exonic regions and their intronic sequences were amplified by PCR and subjected to SSCP and DNA sequencing analyses.


RESULTS
None of the patients was identified to carry any truncating or sequence alterations in MYH. Our screening for the mutational regions, which were recognized from Caucasian patients or affected Indian families, also failed to detect sequence substitutions.


CONCLUSIONS
Mutation in MYH may be rarely involved in the pathogenesis of multiple sporadic colorectal adenomas in Korean population, although a large-scale analysis will be required to clarify the presence of specific MYH variants in a subset of patients and their role in the predisposition of multiple colorectal adenomas in Korean population.
Kim
Hansoo
H
Department of Medicine, Kyung Hee University School of Medicine, #1 Hoegi-Dong, Dongdaemun-Gu, Seoul 130-702, Korea.
Kim
Hyo-Jong
HJ
Chi
Sung-Gil
SG
Lee
Sang-Kil
SK
Joo
Gwang-Ro
GR
Dong
Seok-Ho
SH
Kim
Byung-Ho
BH
Chang
Young-Woon
YW
Lee
Jung-Il
JI
Chang
Rin
R
D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D001483:Base Sequence; D045647:DNA Glycosylases / Q000235:genetics*; D017931:DNA Primers; D005091:Exons; D005260:Female; D006801:Humans; D007438:Introns; D007723:Korea; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D017931:DNA Primers; D045647:DNA Glycosylases; C061738:mutY adenine glycosylase