title: Removal or maintenance of inositol-linked acyl chain in glycosylphosphatidylinositol is critical in trypanosome life cycle.
abstract: The protozoan parasite Trypanosoma brucei is coated by glycosylphosphatidylinositol (GPI)-anchored proteins. During GPI biosynthesis, inositol in phosphatidylinositol becomes acylated. Inositol is deacylated prior to attachment to variant surface glycoproteins in the bloodstream form, whereas it remains acylated in procyclins in the procyclic form. We have cloned a T. brucei GPI inositol deacylase (GPIdeAc2). In accordance with the acylation/deacylation profile, the level of GPIdeAc2 mRNA was 6-fold higher in the bloodstream form than in the procyclic form. Knockdown of GPIdeAc2 in the bloodstream form caused accumulation of an inositol-acylated GPI, a decreased VSG expression on the cell surface and slower growth, indicating that inositol-deacylation is essential for the growth of the bloodstream form. Overexpression of GPIdeAc2 in the procyclic form caused an accumulation of GPI biosynthetic intermediates lacking inositol-linked acyl chain and decreased cell surface procyclins because of release into the culture medium, indicating that overexpression of GPIdeAc2 is deleterious to the surface coat of the procyclic form. Therefore, the GPI inositol deacylase activity must be tightly regulated in trypanosome life cycle.
authors.affiliation: Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Yeonchul
authors.initials: Y
authors.lastname: Hong
authors.forename: Kisaburo
authors.initials: K
authors.lastname: Nagamune
authors.forename: Yasu S
authors.initials: YS
authors.lastname: Morita
authors.forename: Fumiki
authors.initials: F
authors.lastname: Nakatani
authors.forename: Hisashi
authors.initials: H
authors.lastname: Ashida
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Maeda
authors.forename: Taroh
authors.initials: T
authors.lastname: Kinoshita
mesh_terms: D000215:Acylation; D000818:Animals; D003001:Cloning, Molecular; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis* / Q000737:chemistry; D007294:Inositol / Q000378:metabolism*; D008018:Life Cycle Stages; D008562:Membrane Glycoproteins / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D014346:Trypanosoma brucei brucei / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; C053277:procyclic acidic repetitive protein, Trypanosoma; D007294:Inositol; C436709:GPIdeAc protein, Trypanosoma brucei; D010744:Phosphoric Monoester Hydrolases