title: Detection of Newcastle disease virus using nucleic acid sequence-based amplification.
abstract: Newcastle disease (ND) is a contagious and widespread avian disease affecting most species of birds. ND virus (NDV) is the only member of the avian paramyxovirus serotype 1 (APMV1) causing ND outbreak in bird flocks. The technique of nucleic acid sequence-based amplification (NASBA) is a potential method to rapidly and reliably detect NDV isolates. Here, we describe an effective and unprecedented method for detecting NDV strains of all pathotypes. A conserved region of the fusion protein gene was used for designing oligonucleotides specific to all NDV pathotypes. The dynamic range of this NDV NASBA detection method is comparable to virus culture and therefore the NDV NASBA method is a potential alternative for NDV screening and surveillance.
authors.affiliation: National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, No. 427 Maduan Street, Harbin 150001, China.
authors.forename: Shang-Jin
authors.initials: SJ
authors.lastname: Cui
authors.forename: Yin-Wan Wendy
authors.initials: YW
authors.lastname: Fung
authors.forename: Lok Ting
authors.initials: LT
authors.lastname: Lau
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Liu
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Wang
authors.forename: Guang-Zhi
authors.initials: GZ
authors.lastname: Tong
authors.forename: Jianguo
authors.initials: J
authors.lastname: Chen
authors.forename: Albert Cheung Hoi
authors.initials: AC
authors.lastname: Yu
mesh_terms: D000818:Animals; D001717:Birds; D002645:Chickens; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics*; D005784:Gene Amplification; D009521:Newcastle Disease / Q000175:diagnosis*; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010641:Phenotype; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral