title: Rodent adrenocortical cells display high affinity binding sites and proteins for inhibin A, and express components required for autocrine signalling by activins and bone morphogenetic proteins.
abstract: Inhibins are expressed in the adrenal cortex, but little is known of their binding or role in the adrenal. The aims of the present study were, first, to establish whether a mouse adrenocortical (AC) cell line expresses inhibins/activins and bone morphogenetic proteins (BMP), along with proteins required for inhibin to antagonise activin and BMP actions and, secondly, to characterise and compare inhibin binding sites and proteins in the rat adrenal gland and AC cells. AC cells were found to: (1) express mRNA for multiple BMPs (BMP-2, -3, -4, -6, -8a), growth/differentiation factors (GDF-1, -3, -5, -9), Lefty A and B, and the inhibin alpha, beta(A) and beta(B) subunits (2) secrete inhibin A and inhibin B and (3) express mRNA encoding the inhibin co-receptor, betaglycan, along with activin and BMP type I (ALK2-7) and type II (ActRII, ActRIIB, BMPRII) receptors, and binding proteins (follistatin, BAMBI, gremlin). When applied to sections of rat adrenal glands, [(125)I]inhibin A specifically bound to cells of the adrenal cortex, mainly in the zona reticularis. Scatchard analyses of in vitro [(125)I]inhibin A binding to dispersed rat adrenal cells and AC cells revealed sites of high affinity (K(d)(1) of 0.18 and 0.15 nM, respectively) and low affinity (K(d)(2) of 2.6 and 1.3 nM, respectively. Competition for [(125)I]inhibin A binding by activin A or B (30 nM) was negligible, whereas BMP-2, -6 and -7 competed for between 21 and 33% of specific inhibin A binding (IC(50) between 0.2 and 0.3 nM). Inhibin B crossreaction with inhibin A binding sites was < 8%. Multiple binding protein complexes (molecular weight ranging from 35 to > 220 kDa) were affinity labelled by [(125)I]inhibin A on both the primary rat adrenal and AC cells. The species of > 220 kDa were shown by immunoprecipitation to include betaglycan, the species of 105 kDa is consistent in size with type II receptors for activin/BMP, and that of 62 kDa co-migrates with the inhibin-follistatin complex. In summary, the results show that inhibin A binds selectively and with both high and low affinity to AC cells via multiple binding proteins, including a single betaglycan-like species. The results support the role of glycosylated betaglycan in the high affinity binding of inhibin A, but provide consistent evidence from two independent sources of adrenal cells that inhibin A interacts with several membrane proteins in addition to those currently understood to mediate the anti-activin/BMP actions of inhibin.
authors.affiliation: Prince Henry's Institute of Medical Research, PO Box 5152, Clayton, 3168 Victoria, Australia. paul.farnworth@princehenrys.org
authors.forename: Paul G
authors.initials: PG
authors.lastname: Farnworth
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.forename: Pauline
authors.initials: P
authors.lastname: Leembruggen
authors.forename: Guck T
authors.initials: GT
authors.lastname: Ooi
authors.forename: Craig
authors.initials: C
authors.lastname: Harrison
authors.forename: David M
authors.initials: DM
authors.lastname: Robertson
authors.forename: Jock K
authors.initials: JK
authors.lastname: Findlay
mesh_terms: D028341:Activins / Q000378:metabolism*; D000302:Adrenal Cortex / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D019898:Autocrine Communication / Q000502:physiology*; D001665:Binding Sites; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D047468:Immunoprecipitation; D007265:Inhibins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis* / Q000235:genetics; D007553:Isotope Labeling; D008297:Male; D011485:Protein Binding; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019485:Bone Morphogenetic Proteins; D047908:Intracellular Signaling Peptides and Proteins; C422362:inhibin A; C422364:inhibin B; C094377:inhibin-alpha subunit; D028341:Activins; D007265:Inhibins