Novel fluorescent oligoDNA probe bearing a multi-conjugated nucleoside with a fluorophore and a non-fluorescent intercalator as a quencher.
A set of 15mer linear oligoDNA probes bearing a modified nucleoside conjugated with a polyamine/fluorescein/anthraquinone reporting moiety were synthesized. In a single-stranded form, the fluorescence generated by the excitation of fluorescein was efficiently quenched, while marked recovery of the fluorescence was observed when the probes formed duplexes with the fully complementary strand.
Kodama
Sinichi
S
Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu 376-8515, Japan.
Asano
Satoko
S
Moriguchi
Tomohisa
T
Sawai
Hiroaki
H
Shinozuka
Kazuo
K
D015342:DNA Probes; D005456:Fluorescent Dyes / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D015342:DNA Probes; D005456:Fluorescent Dyes; D007364:Intercalating Agents