title: Effect of organogenesis on steroidal saponin biosynthesis in calli cultures of Ruscus aculeatus.
abstract: Calli cultures derived from Ruscus aculeatus rhizomes were investigated for their potential to biosynthesize saponins. The capacity of undifferentiated tissues to form steroidal saponins is very limited, but when the calli developed organogenesis, mainly aerial shoots and roots, the saponin production increased significantly. Plantlets regenerated from aerial shoots of Ruscus calli showed a saponin pattern similar to that of the callus cultures but the levels of saponins found in the aerial part and roots were significantly greater.
authors.affiliation: Laboratorio de Fisiología Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avenida Diagonal 643, 08028 Barcelona, Spain. javierpalazon@ub.edu
authors.forename: Javier
authors.initials: J
authors.lastname: Palazón
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Moyano
authors.forename: Mercedes
authors.initials: M
authors.lastname: Bonfill
authors.forename: Lidia T
authors.initials: LT
authors.lastname: Osuna
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Cusidó
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Piñol
mesh_terms: D018533:Biomass; D038081:Organogenesis / Q000502:physiology*; D010936:Plant Extracts / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry; D012038:Regeneration; D027343:Rhizome / Q000502:physiology; D031443:Ruscus / Q000737:chemistry* / Q000254:growth & development / Q000502:physiology*; D012503:Saponins / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D010936:Plant Extracts; D012503:Saponins