Local InsP3-dependent perinuclear Ca2+ signaling in cardiac myocyte excitation-transcription coupling.
Previous work showed that calmodulin (CaM) and Ca2+-CaM-dependent protein kinase II (CaMKII) are somehow involved in cardiac hypertrophic signaling, that inositol 1,4,5-trisphosphate receptors (InsP3Rs) in ventricular myocytes are mainly in the nuclear envelope, where they associate with CaMKII, and that class II histone deacetylases (e.g., HDAC5) suppress hypertrophic gene transcription. Furthermore, HDAC phosphorylation in response to neurohumoral stimuli that induce hypertrophy, such as endothelin-1 (ET-1), activates HDAC nuclear export, thereby regulating cardiac myocyte transcription. Here we demonstrate a detailed mechanistic convergence of these 3 issues in adult ventricular myocytes. We show that ET-1, which activates plasmalemmal G protein-coupled receptors and InsP3 production, elicits local nuclear envelope Ca2+ release via InsP3R. This local Ca2+ release activates nuclear CaMKII, which triggers HDAC5 phosphorylation and nuclear export (derepressing transcription). Remarkably, this Ca2+-dependent pathway cannot be activated by the global Ca2+ transients that cause contraction at each heartbeat. This novel local Ca2+ signaling in excitation-transcription coupling is analogous to but separate (and insulated) from that involved in excitation-contraction coupling. Thus, myocytes can distinguish simultaneous local and global Ca2+ signals involved in contractile activation from those targeting gene expression.
Wu
Xu
X
Loyola University Chicago, Chicago, Illinois 60153, USA.
Zhang
Tong
T
Bossuyt
Julie
J
Li
Xiaodong
X
McKinsey
Timothy A
TA
Dedman
John R
JR
Olson
Eric N
EN
Chen
Ju
J
Brown
Joan Heller
JH
Bers
Donald M
DM
D021581:Active Transport, Cell Nucleus / Q000502:physiology; D000818:Animals; D015220:Calcium Channels / Q000172:deficiency / Q000235:genetics; D020013:Calcium Signaling / Q000502:physiology*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D002147:Calmodulin / Q000378:metabolism; D002478:Cells, Cultured; D019332:Endothelin-1 / Q000502:physiology; D006655:Histone Deacetylases / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate / Q000502:physiology*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D051379:Mice; D018345:Mice, Knockout; D032383:Myocytes, Cardiac / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D009685:Nuclear Envelope / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D011817:Rabbits; D018160:Receptors, Cytoplasmic and Nuclear / Q000172:deficiency / Q000235:genetics; D014158:Transcription, Genetic / Q000502:physiology*
D015220:Calcium Channels; D002147:Calmodulin; D019332:Endothelin-1; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D015544:Inositol 1,4,5-Trisphosphate; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C498618:Hdac5 protein, mouse; D006655:Histone Deacetylases