The biosynthetic gene cluster for the beta-lactam antibiotic tabtoxin in Pseudomonas syringae.
DNA sequence analysis revealed that the biosynthetic genes of the unusual beta-lactam antibiotic tabtoxin reside at the att site adjacent to the lysC tRNA gene in Pseudomonas syringae BR2. ORFs encoded within the region included ones with similarity to beta-lactam synthase and clavaminic acid synthase, as well as amino acid synthesis enzymes. Novel ORFs were present in a portion of the biosynthetic region associated with a toxin hypersensitivity phenotype. Tabtoxin resistance was associated with a fragment containing a major facilitator superfamily (MFS) transporter gene.
Kinscherf
Thomas G
TG
USDA/ARS Vegetable Crops Research Unit and Department of Plant Pathology, University of Wisconsin - Madison, Madison, WI 53706, USA.
Willis
David K
DK
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial; D004151:Dipeptides / Q000096:biosynthesis* / Q000235:genetics*; D005810:Multigene Family; D010957:Plasmids / Q000235:genetics; D044224:Pseudomonas syringae / Q000235:genetics* / Q000378:metabolism*; D012343:RNA, Transfer / Q000096:biosynthesis / Q000235:genetics
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004151:Dipeptides; D012343:RNA, Transfer; C003854:tabtoxin