title: Novel fluorescent oligoDNA probe bearing a multi-conjugated nucleoside with a fluorophore and a non-fluorescent intercalator as a quencher.
abstract: A set of 15mer linear oligoDNA probes bearing a modified nucleoside conjugated with a polyamine/fluorescein/anthraquinone reporting moiety were synthesized. In a single-stranded form, the fluorescence generated by the excitation of fluorescein was efficiently quenched, while marked recovery of the fluorescence was observed when the probes formed duplexes with the fully complementary strand.
authors.affiliation: Department of Chemistry, Faculty of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu 376-8515, Japan.
authors.forename: Sinichi
authors.initials: S
authors.lastname: Kodama
authors.forename: Satoko
authors.initials: S
authors.lastname: Asano
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Moriguchi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sawai
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozuka
mesh_terms: D015342:DNA Probes; D005456:Fluorescent Dyes / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D007364:Intercalating Agents