title: Thiolate exchange in [TmR]ZnSR' complexes and relevance to the mechanisms of thiolate alkylation reactions involving zinc enzymes and proteins.
abstract: The zinc and cadmium thiolate complexes [TmBut]MSCH2C(O)N(H)Ph (M = Zn, Cd) may be obtained via treatment of the respective methyl complex [TmBut]MMe with PhN(H)C(O)CH2SH. The molecular structure of [TmBut]ZnSCH2C(O)N(H)Ph has been determined by X-ray diffraction, thereby demonstrating the presence of an intramolecular N-H S hydrogen bond between the amide N-H group and thiolate sulfur atom. [TmBut]ZnSCH2C(O)N(H)Ph mimics the function of the Ada DNA repair protein by undergoing alkylation with MeI to give [TmBut]ZnI and MeSCH2C(O)N(H)Ph. A series of crossover experiments and 1H NMR magnetization transfer studies establish that thiolate exchange between [TmR]ZnSR' derivatives is facile in this system, an observation that supports the previous suggestion that the alkylation of [TmPh]ZnSCH2C(O)N(H)Ph by MeI may proceed via a sequence that involves dissociation of [PhN(H)C(O)CH2S]-.
authors.affiliation: Department of Chemistry, Columbia University, 3000 Broadway, MC 3155, New York, NY 10027, USA.
authors.forename: Jonathan G
authors.initials: JG
authors.lastname: Melnick
authors.forename: Guang
authors.initials: G
authors.lastname: Zhu
authors.forename: Daniela
authors.initials: D
authors.lastname: Buccella
authors.forename: Gerard
authors.initials: G
authors.lastname: Parkin
mesh_terms: D000478:Alkylation; D004798:Enzymes / Q000737:chemistry*; D008958:Models, Molecular; D011506:Proteins / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: D004798:Enzymes; D011506:Proteins; D013438:Sulfhydryl Compounds; D015032:Zinc