title: Release of interleukin-6 via the regulated secretory pathway in PC12 cells.
abstract: A growing body of evidence suggests that diverse growth factors such as neurotrophins (NTs), insulin-like growth factor-1 (IGF-1), and glial cell line-derived neurotrophic factor (GDNF) can be released via the regulated secretory pathway in neuronal cells, possibly representing a mechanism for preferentially supplying these growth factors to active synapses. Here we investigated whether interleukin-6 (IL-6), a member of the family of neuropoietic cytokines, can be released via stimulus-coupled secretion as well. IL-6 was expressed in PC12 cells, a neuronal model cell line that is frequently used for the study of vesicle release and trafficking. Regulated secretion of this cytokine was induced by 0.5 mM ATP and treatment with epidermal growth factor (EGF) and nerve growth factor (NGF). Release induced by 0.5 mM ATP but not by NGF or EGF depended on the presence of extracellular Ca(++). Furthermore, IL-6 colocalized with the dense core vesicle (DCV)-marker secretogranin-II (Sg-II) in transfected PC12 cells. Our data suggest that the neuropoietic cytokine IL-6 can be sorted to the regulated secretory pathway in neuronal cells and indicate a potential role for this cytokine in synaptic plasticity.
authors.affiliation: Department of Neurology, Philipps-University, Rudolf-Bultmann-Str. 8, D-35039 Marburg, Germany. moellerc@staff.uni-marburg.de
authors.forename: Jens Carsten
authors.initials: JC
authors.lastname: Möller
authors.forename: Alex
authors.initials: A
authors.lastname: Krüttgen
authors.forename: Rosi
authors.initials: R
authors.lastname: Burmester
authors.forename: Joachim
authors.initials: J
authors.lastname: Weis
authors.forename: Wolfgang H
authors.initials: WH
authors.lastname: Oertel
authors.forename: Eric M
authors.initials: EM
authors.lastname: Shooter
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002864:Chromogranins / Q000378:metabolism; D004347:Drug Interactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004815:Epidermal Growth Factor / Q000494:pharmacology; D007150:Immunohistochemistry / Q000379:methods; D015850:Interleukin-6 / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000494:pharmacology; D016716:PC12 Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D022142:Secretory Vesicles / Q000187:drug effects* / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: D002864:Chromogranins; D015850:Interleukin-6; D004815:Epidermal Growth Factor; D000255:Adenosine Triphosphate; D020932:Nerve Growth Factor; D002118:Calcium