Vesicular glutamate transporter 3 (VGLUT3) identifies spatially segregated excitatory terminals in the rat substantia nigra.
The excitability of dopaminergic (DA) neurons in the substantia nigra is controlled by the convergent activity of multiple glutamatergic afferents. Here, we show that vesicular glutamate transporter 3 (VGLUT3)-immunoreactive (ir) terminals segregate to the perisomatic region of DA neurons in the substantia nigra pars compacta, and VGLUT3 decorates a synapse population distinct from those marked by vesicular glutamate transporters 1 and 2. VGLUT3-ir nerve endings form asymmetric terminals on DA neurons. Retrograde tracing suggests the superior colliculus as an origin of excitatory VGLUT3-ir afferents. Collectively, our data indicate that VGLUT3 identifies a novel excitatory terminal subset that contributes to the tuning of DA cell excitability in the substantia nigra.
Martín-Ibañez
Raquel
R
Laboratory for Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Scheeles väg 1:A1.
Jenstad
Monica
M
Berghuis
Paul
P
Edwards
Robert H
RH
Hioki
Hiroyuki
H
Kaneko
Takeshi
T
Mulder
Jan
J
Canals
Josep M
JM
Ernfors
Patrik
P
Chaudhry
Farrukh A
FA
Harkany
Tibor
T
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D002867:Chromones; D003911:Dextrans / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods; D016253:Microscopy, Immunoelectron / Q000379:methods; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013378:Substantia Nigra / Q000166:cytology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D050988:Vesicle-Associated Membrane Protein 2 / Q000378:metabolism; D050599:Vesicular Glutamate Transport Protein 2 / Q000378:metabolism; D050496:Vesicular Glutamate Transport Proteins / Q000378:metabolism*
C470766:5-hydroxy-2-(14'-nonadecenyl) chromone; D002867:Chromones; D003911:Dextrans; D043884:Receptor, Cannabinoid, CB1; C482103:Slc17a8 protein, rat; D050988:Vesicle-Associated Membrane Protein 2; D050599:Vesicular Glutamate Transport Protein 2; D050496:Vesicular Glutamate Transport Proteins; C076397:biotinylated dextran amine; D012701:Serotonin; D001710:Biotin; D014446:Tyrosine 3-Monooxygenase; D010751:Phosphopyruvate Hydratase