Development and validation of a method for the quantitation of Delta9 tetrahydrocannabinol in human plasma by high performance liquid chromatography after solid-phase extraction.
A high performance liquid chromatography (HPLC) procedure for the determination of Delta9 tetrahydrocannabinol (THC) in human plasma is described. A two-step solid-phase extraction on CN cartridges was coupled with a reversed phase HPLC system. THC was eluted using a mobile phase composed of methanol, acetonitrile and tetrabutylammonium perchlorate solution (0.005 M, pH 3.2), through a C18 Nucleosil column and detected at a wavelength of 215 nm. Calibration curve was linear over the range 5-100 ng/ml with a lower limit of quantification validated at 5 ng/ml. Extraction recovery using the developed extraction procedure was higher than 85%. This method is presently used for the quantification of THC in plasma samples from regular cannabis smokers.
Abbara
Chadi
C
Laboratoire de Pharmacologie, Service de Pharmacie, Hôpital Paul BROUSSE (AP-HP), 12, Avenue Paul Vaillant Couturier, 94800 Villejuif, France. chadi.abbara@pbr.ap-hop-paris.fr
Galy
Romain
R
Benyamina
Amine
A
Reynaud
Michel
M
Bonhomme-Faivre
Laurence
L
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013759:Dronabinol / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D013759:Dronabinol