title: New bioluminogenic substrates for monoamine oxidase assays.
abstract: Novel bioluminogenic substrates were designed for probing monoamine oxidase (MAO) activity based on a simple and effective beta-elimination strategy. By modifying the amino group and the central core of luciferin derivatives, we have developed a series of substrates useful for assays of MAO A or B, or both. One of these substrates, exhibiting low Km values and high signal-to-background ratios with both isozymes, was shown to accurately measure the Ki values of known MAO inhibitors. This substrate is a key component in the development of a highly sensitive homogeneous MAO assay for high-throughput screening (HTS) of compounds in drug discovery and for monitoring MAO activity in complex biological systems. This design strategy should be applicable to fluorogenic MAO substrates and could broaden the structural requirements of substrates for other enzyme assays.
authors.affiliation: Promega Biosciences, Inc., 277 Granada Drive, San Luis Obispo, California 93401, USA. wenhui.zhou@promega.com
authors.forename: Wenhui
authors.initials: W
authors.lastname: Zhou
authors.forename: Michael P
authors.initials: MP
authors.lastname: Valley
authors.forename: John
authors.initials: J
authors.lastname: Shultz
authors.forename: Erika M
authors.initials: EM
authors.lastname: Hawkins
authors.forename: Laurent
authors.initials: L
authors.lastname: Bernad
authors.forename: Troy
authors.initials: T
authors.lastname: Good
authors.forename: Dave
authors.initials: D
authors.lastname: Good
authors.forename: Terry L
authors.initials: TL
authors.lastname: Riss
authors.forename: Dieter H
authors.initials: DH
authors.lastname: Klaubert
authors.forename: Keith V
authors.initials: KV
authors.lastname: Wood
mesh_terms: D049454:Firefly Luciferin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism; D007700:Kinetics; D049408:Luminescent Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D008995:Monoamine Oxidase / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D049408:Luminescent Agents; D049454:Firefly Luciferin; D008995:Monoamine Oxidase