[In vivo comparison of preservation of murine islets of Langerhans].
OBJECTIVE
A new preservation solution containing polyethyleneglycol (PEG) was studied. The length of the PEG chain determines its biological properties. The objective of this study was to define the optimal length of PEG chains to obtain the best tissue preservation.


METHODS
A murine model of islet of Langerhans transplantation was used. Solutions containing 8 kDa, 20 kDa or 35 kDa PEG at a molar concentration of 1.5 mM were compared to HBSS. Primary non-function (PNF) and mean graft survival rates were studied in these 4 groups.


RESULTS
Better preservation was obtained with 20 kDa PEG than with 8 kDa or 35 kDa (PNF rates of 0% vs 56% vs 37%, respectively). The difference with HBSS (22% PNF) was not significant. Graft survival was also longer with 20 kDa PEG than with 35 kDa PEG (23.1 +/- 4.4 days vs 8.6 +/- 7.6 days). Differences with HBSS (13.4 +/- 11.8 days) and 8 kDa PEG (12.2 +/- 14.7 days) were not significant.


CONCLUSIONS
The best preservation was obtained with 20 kDa PEG at a molar concentration of 1.5 mM. Other PEG concentrations need to be studied.
Neuzillet
Yann
Y
Faculté de Médecine Pitié-Salpêtrière, Université Pierre et Marie Curie (Paris VI), Laboratoire d'Immunologie Cellulaire et Tissulaire, INSERM U543, Paris, France.
Giraud
Sébastien
S
Lagorce
Lorraine
L
Eugène
Michel
M
Richard
François
F
Debrè
Patrice
P
Barrou
Benoît
B
D000818:Animals; D007515:Islets of Langerhans; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D019188:Organ Preservation Solutions
D019188:Organ Preservation Solutions