title: Lactobacillus nantensis sp. nov., isolated from French wheat sourdough.
abstract: A polyphasic taxonomic study of the bacterial flora isolated from traditional French wheat sourdough, using phenotypic characterization and phylogenetic as well as genetic methods, revealed a consistent group of isolates that could not be assigned to any recognized species. These results were confirmed by randomly amplified polymorphic DNA and amplified fragment length polymorphism fingerprinting analyses. Cells were Gram-positive, homofermentative rods. Comparative 16S rRNA gene sequence analysis of the representative strain LP33T indicated that these strains belong to the genus Lactobacillus and that they formed a branch distinct from their closest relatives Lactobacillus farciminis, Lactobacillus alimentarius, Lactobacillus paralimentarius and Lactobacillus mindensis. DNA-DNA reassociation experiments with the three phylogenetically closest Lactobacillus species confirmed that LP33T (= DSM 16982T = CIP 108546T = TMW 1.1265T) represents the type strain of a novel species, for which the name Lactobacillus nantensis sp. nov. is proposed.
authors.affiliation: Department of Microbiology, Faculty of Biology, University Sofia, 8 Dragan Tzankov Street, 1162 Sofia, Bulgaria.|Laboratoire de Microbiologie Alimentaire et Industrielle (LMAI), Unité de Recherche QM2A, ENITIAA, rue de la Géraudière, BP 82225, 44322 Nantes Cedex 3, France.
authors.forename: Rosica
authors.initials: R
authors.lastname: Valcheva
authors.affiliation: Laboratoire de Microbiologie Alimentaire et Industrielle (LMAI), Unité de Recherche QM2A, ENITIAA, rue de la Géraudière, BP 82225, 44322 Nantes Cedex 3, France.
authors.forename: Mounir F
authors.initials: MF
authors.lastname: Ferchichi
authors.affiliation: Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, 85350 Freising, Germany.
authors.forename: Maher
authors.initials: M
authors.lastname: Korakli
authors.affiliation: Department of Microbiology, Faculty of Biology, University Sofia, 8 Dragan Tzankov Street, 1162 Sofia, Bulgaria.
authors.forename: Iskra
authors.initials: I
authors.lastname: Ivanova
authors.affiliation: Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, 85350 Freising, Germany.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Gänzle
authors.affiliation: Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, 85350 Freising, Germany.
authors.forename: Rudi F
authors.initials: RF
authors.lastname: Vogel
authors.affiliation: Laboratoire de Microbiologie Alimentaire et Industrielle (LMAI), Unité de Recherche QM2A, ENITIAA, rue de la Géraudière, BP 82225, 44322 Nantes Cedex 3, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Prévost
authors.affiliation: Laboratoire de Microbiologie Alimentaire et Industrielle (LMAI), Unité de Recherche QM2A, ENITIAA, rue de la Géraudière, BP 82225, 44322 Nantes Cedex 3, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Onno
authors.affiliation: Laboratoire de Microbiologie Alimentaire et Industrielle (LMAI), Unité de Recherche QM2A, ENITIAA, rue de la Géraudière, BP 82225, 44322 Nantes Cedex 3, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Dousset
mesh_terms: D001939:Bread / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000737:chemistry; D005285:Fermentation; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000382:microbiology*
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S