Superoxide anion as a marker of ischemia-reperfusion injury of the transplanted kidney.
Reactive oxygen species (ROS) are considered to be important factors involved in the pathophysiology of renal ischemia-reperfusion injury. ROS-induced alterations of proteins, carbohydrates, DNA, and lipid membranes lead to cell and organ dysfunction. Several antioxidant defense mechanisms exist to prevent or limit oxidant injury. Cellular Cu-Zn superoxide dismutase, catalase, and cellular glutathione peroxidase (cGSH-Px) are enzyme ROS scavengers, implicated in the protection against kidney damage resulting from ischemia-reperfusion injury. Reduced glutathione, a cosubstrate of cGSH-Px, have been shown to display a reductive properties without the contribution of enzymes. We examined superoxide anion (O(2)(-)) production by neutrophils, without and with stimulation using opsonized zymosan, in the whole blood of renal transplant patients before and after (5 and 15 minutes) reperfusion. The mean O(2)(-) concentration after reperfusion was statistically significantly higher than that before reperfusion.
Masztalerz
M
M
Department of Transplantology, Nicolaus Copernicus University, Collegium Medicum, ul Skłodowskiej-Curie 9, 85-094 Bydgoszcz, Poland. masztalerzm@wp.pl
Włodarczyk
Z
Z
Czuczejko
J
J
Słupski
M
M
Kedziora
J
J
D000328:Adult; D015415:Biomarkers / Q000097:blood; D016166:Free Radical Scavengers; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology; D017382:Reactive Oxygen Species; D015427:Reperfusion Injury / Q000097:blood*; D013481:Superoxides / Q000097:blood*
D015415:Biomarkers; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D013481:Superoxides