title: PCL-PU composite vascular scaffold production for vascular tissue engineering: attachment, proliferation and bioactivity of human vascular endothelial cells.
abstract: A new compliant scaffold suitable for small-diameter vascular grafts has been developed that promotes strong attachment of endothelial cells. Composite scaffolds were produced by wet spinning polycaprolactone (PCL) fibres which form the luminal surface, then electrospinning porous polyurethane (PU) onto the back of the PCL fibres to form the vessel wall substitute. Human endothelial cells demonstrated strong attachment to the composite PCL-PU scaffold, and proliferated to form a monolayer with strong PECAM-1 expression and cobblestone morphology. Attached cells demonstrated abundant release of von Willebrand factor, nitric oxide and ICAM-1 under physiological stimuli, and exhibited an immune response to lipopolysaccharide. The composite scaffold may also deliver bioactive molecules. Active trypsin, used as a test molecule, had a defined 48 h pattern of release from luminal PCL fibres. These data confirm the potential of this novel composite scaffold in vascular tissue engineering.
authors.affiliation: UK Centre for Tissue Engineering, University of Manchester, Oxford Rd, Manchester M13 9PT, UK. matthew.williamson@manchester.ac.uk
authors.forename: Matthew Richard
authors.initials: MR
authors.lastname: Williamson
authors.forename: Richard
authors.initials: R
authors.lastname: Black
authors.forename: Cay
authors.initials: C
authors.lastname: Kielty
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007797:Laminin / Q000235:genetics; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009569:Nitric Oxide / Q000378:metabolism; D011091:Polyesters; D011140:Polyurethanes; D023822:Tissue Engineering / Q000379:methods*; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D007797:Laminin; D011091:Polyesters; D011140:Polyurethanes; C097043:laminin alpha5; D014841:von Willebrand Factor; D018799:Intercellular Adhesion Molecule-1; C016240:polycaprolactone; D009569:Nitric Oxide