title: A simple method for constructing a tagged protein.
abstract: We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.
authors.affiliation: Department of Biomedical Sciences, National Institute of Health, Seoul [122-701], Korea.
authors.forename: C
authors.initials: C
authors.lastname: Jo
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: S A
authors.initials: SA
authors.lastname: Jo
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005818:Genetic Engineering / Q000379:methods*; D051379:Mice; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D025842:SUMO-1 Protein