title: Human colostrum: identification of minor proteins in the aqueous phase by proteomics.
abstract: Human colostrum is an important source of protective, nutritional and developmental factors for the newborn. We have investigated the low abundance proteins in the aqueous phase of human colostrum, after depletion of the major proteins secretory IgA, lactoferrin, alpha-lactalbumin and HSA by immunoabsorption, using 2-D LC and gel-based proteomic methods. One hundred and fifty-one proteins were identified, 83 of which have not been previously reported in human colostrum, or milk. This is the first comprehensive proteomic analysis of human colostrum produced during the first 48 h of lactation.
authors.affiliation: LactoPharma, School of Biological Sciences, University of Auckland, Auckland, New Zealand.
authors.forename: David J
authors.initials: DJ
authors.lastname: Palmer
authors.forename: Van C
authors.initials: VC
authors.lastname: Kelly
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Smit
authors.forename: Sulee
authors.initials: S
authors.lastname: Kuy
authors.forename: Catriona G
authors.initials: CG
authors.lastname: Knight
authors.forename: Garth J
authors.initials: GJ
authors.lastname: Cooper
mesh_terms: D003126:Colostrum / Q000737:chemistry* / Q000201:enzymology; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000737:chemistry; D007144:Immunoglobulin J-Chains / Q000737:chemistry; D007147:Immunoglobulin Light Chains / Q000737:chemistry; D007768:Lactalbumin / Q000737:chemistry; D007781:Lactoferrin / Q000737:chemistry; D011247:Pregnancy; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D040901:Proteomics; D012709:Serum Albumin / Q000737:chemistry; D014867:Water
chemical_list: D007071:Immunoglobulin A, Secretory; D007144:Immunoglobulin J-Chains; D007147:Immunoglobulin Light Chains; D011506:Proteins; D020543:Proteome; D012709:Serum Albumin; D014867:Water; D007768:Lactalbumin; D007781:Lactoferrin