Cloning and embryonic expression of five distinct sfrp genes in the zebrafish Danio rerio.
Recently, a new member of the secreted frizzled-related protein (sFRP) family, named tlc, has been identified as expressed by the anterior neural border (ANB) cells in the zebrafish Danio rerio. Tlc plays an important role in telencephalic induction and patterning. In absence of Tlc, formation of the telencephalon is severely delayed, but not abolished. This prompted us to clone the other zebrafish sfrp family members and analyse their expression patterns, in search of a family member that may partly functionally overlap with Tlc. Except sizzled, expression profile of sfrp genes in zebrafish has not been reported so far. Here, we describe the cloning of full-length cDNA for sfrp1a, sfrp1b, sfrp2, sfrp3 and sfrp5 gene transcripts and we examine their expression at different embryonic stages. Only sfrp1a is expressed in the anterior neural plate including the ANB cells where and when tlc is expressed. Interestingly, compared to both tlc and sfrp1a, wnt genes are complementary expressed more posteriorly in the neural plate. Later, both sfrp1a and sfrp5 expression profiles are overlapping, in particular at pharyngula stage these genes are expressed in the ventral part of the forebrain, midbrain and hindbrain. sfrp1b, sfrp2 and sfrp3 are mainly expressed in mesodermal and endodermal embryonic tissues. Expression profiles of these different genes in zebrafish gave interesting clues on the possible function and evolution of sFRPs in zebrafish and other organisms.
Tendeng
Christian
C
MRC Centre for Developmental Neurobiology, New Hunt's House, King's College London, London SE1 9RT, United Kingdom.
Houart
Corinne
C
D000818:Animals; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D017124:Conserved Sequence; D004625:Embryo, Nonmammalian; D019143:Evolution, Molecular; D005775:Gastrula / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D010802:Phylogeny; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D019170:Somites / Q000378:metabolism; D013116:Spinal Cord / Q000196:embryology / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D029961:Zebrafish Proteins / Q000378:metabolism; D000099274:Frizzled-Related Proteins; D000094742:Secreted Frizzled-Related Proteins
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D029961:Zebrafish Proteins; D000099274:Frizzled-Related Proteins; C515514:Sfrp1a protein, zebrafish; C515515:Sfrp1b protein, zebrafish; C515511:Sfrp2 protein, zebrafish; D000094742:Secreted Frizzled-Related Proteins; C514540:Sfrp5 protein, zebrafish; C515510:frzb protein, zebrafish