Genetic evidence for the involvement of Fcgamma receptor III in experimental autoimmune myasthenia gravis pathogenesis.
Immune complexes and classical complement pathway play vital roles in experimental autoimmune myasthenia gravis (EAMG). To analyze the role of immune complex receptors in EAMG, FcgammaRIII knockout (KO) mice were immunized with AChR and were found out to be resistant to EAMG induction. This was associated with reduced neuromuscular junction deposits, lymph node cell (LNC) IL-6 production and serum complement levels. EAMG resistance of anti-C1q Ab-administered mice was also associated with reduced LNC IL-6 production and neuromuscular junction deposits, indicating C1q involvement in EAMG resistance. The data provide the first direct genetic evidence for Fcgamma receptor involvement in EAMG pathogenesis.
Tüzün
Erdem
E
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555-1070, USA.
Saini
Shamsher S
SS
Yang
Huan
H
Alagappan
Dhivyaa
D
Higgs
Stephen
S
Christadoss
Premkumar
P
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D015703:Antigens, CD / Q000378:metabolism; D015922:Complement C1q / Q000276:immunology / Q000378:metabolism; D050577:Complement C3-C5 Convertases / Q000378:metabolism; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D007074:Immunoglobulin G / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D015850:Interleukin-6 / Q000378:metabolism; D008198:Lymph Nodes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000503:physiopathology; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology; D009469:Neuromuscular Junction / Q000378:metabolism / Q000473:pathology; D011863:Radioimmunoassay / Q000379:methods; D017452:Receptors, IgG / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D011978:Receptors, Nicotinic / Q000235:genetics; D018709:Statistics, Nonparametric; D013997:Time Factors
D000906:Antibodies; D015703:Antigens, CD; D015938:Complement Membrane Attack Complex; C485700:Fcgr3 protein, mouse; D007074:Immunoglobulin G; D015850:Interleukin-6; D017452:Receptors, IgG; D011978:Receptors, Nicotinic; D015922:Complement C1q; D050577:Complement C3-C5 Convertases