[Effects of union of albumin and egg yolk on human semen cryopreservation].
OBJECTIVE
To provide a more effective cryoprotective medium (CPM), effect of union of albumin and egg yolk on human sperm cryopreservation was studied.


METHODS
Egg yolk-glycerol-sodium citrate was regarded as CPM of the control group and egg yolk-glycerol-sodium citrate with different concentrations of albumin (1, 2, 3, 4, 5 g/L) were regarded as CPMs of experiment groups. Before and after cryopreservation, sperm movement parameters were assessed by using computer-aided sperm analyzer (CASA) system, and then egg yolk-glycerol-sodium citrate group added 1 g/L albumin was selected, whose movement parameters were the best among the experimental groups, and egg yolk-glycerol-sodium citrateto group as the control to compare sperm survival rate, membrane integrity, function of mitochondrion and ultrastruction.


RESULTS
Sperm in egg yolk-glycerol-sodium citrate added I g/L albumin showed significantly higher motility, viability than those in the control group and other experimental groups (P < 0.05). Sperm in egg yolk-glycerol-sodium citrate group added 1 g/L albumin had significantly higher survival rate, head unpigmenting rate than those in control group (P < 0.05). Sperm in egg yolk-glycerol-sodium citrate group added I g/L albumin manifested significantly higher succinate dehydrogenase (SDH) activity than that in control group (P < 0.05) and had better ultrastructure than that in control group.


CONCLUSIONS
Union of two kinds of albumin and egg yolk has better effects on human sperm cryopreservation than those of solitary use of egg yolk. The action of albumin is related to its concentration, and albumin combining with egg yolk may have plus and complementary effects on human sperm cryopreservation.
Huang
Dong-Hui
DH
Family Planning Research Institute, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430030, China. jhsyyis@126.com
Zhao
Hu
H
Xiong
Cheng-Liang
CL
Sheng
Ji-Yun
JY
Zhou
Jie-Ling
JL
D000328:Adult; D000418:Albumins; D015925:Cryopreservation; D004530:Egg Yolk; D006801:Humans; D008297:Male; D012662:Semen Preservation / Q000379:methods*
D000418:Albumins