Aortic coarctation induces oxidative stress in rat tissues.
The purpose of this study was to evaluate the induction of oxidative stress in heart and erythrocytes from rats with abdominal aorta coarctation (Coa) compared with sham-operated normotensive controls (Sham). The group of Coa animals developed myocardial hypertrophy, showing heart homogenates markedly increased levels of reduced glutathione (48%), lipid peroxidation (148%) and activation of superoxide dismutase and glutathione peroxidase (189% and 37%, respectively), compared with controls. Other oxidative stress indicators were also altered in erythrocytes from Coa rats: increased protein carbonyl content (141%) and total glutathione level (349%) were determined. Inactivation of the antioxidant enzymes catalase (27%), superoxide dismutase (58%) and glutathione peroxidase (25%) was observed in erythrocytes from the Coa group. Taken jointly our results provide strong evidence for the production of oxidative stress in heart and erythrocytes from aortic coarcted rats.
Polizio
Ariel Héctor
AH
Departamento de Química Biológica, Instituto de Química y Fisicoquímica Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina.
Gorzalczany
Susana
S
Taira
Carlos
C
Peña
Clara
C
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001012:Aorta, Abdominal; D001017:Aortic Coarctation / Q000150:complications / Q000201:enzymology / Q000378:metabolism*; D001835:Body Weight; D006332:Cardiomegaly / Q000201:enzymology / Q000209:etiology / Q000378:metabolism; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D009929:Organ Size; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D008054:Lipid Peroxides; D005978:Glutathione