Purification, crystallization and preliminary X-ray diffraction studies on human Ca2+-binding protein S100B.
S100B, a Ca2+-binding protein, acts intracellularly as a Ca2+-signalling protein but is also secreted to the extracellular space, acting in a cytokine-like manner through its receptor RAGE. Recombinant human S100B has been purified and crystallized in the Ca2+-bound state. Size-exclusion chromatography indicates that S100B can exist as a dimer and as a multimer in solution. Crystals of S100B diffract to 1.9 A and belong to space group P2(1), with unit-cell parameters a = 63.4, b = 81.6, c = 71.5 A, alpha = 90, beta = 107, gamma = 90 degrees. Preliminary analysis of the X-ray data indicate that there are four homodimers per asymmetric unit.
Ostendorp
Thorsten
T
Fachbereich Biologie, Universität Konstanz, Postfach M665, Universitätsstrasse 10, 78467 Konstanz, Germany.
Heizmann
Claus W
CW
Kroneck
Peter M H
PM
Fritz
Günter
G
D002118:Calcium / Q000378:metabolism*; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D016207:Cytokines / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D019281:Dimerization; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009414:Nerve Growth Factors / Q000737:chemistry*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction; D014965:X-Rays
D016207:Cytokines; D018076:DNA, Complementary; D009414:Nerve Growth Factors; D011994:Recombinant Proteins; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human; D002118:Calcium