Development and validation of isomer specific RP-HPLC method for quantification of cefpodoxime proxetil.
The present work explains the development and validation of a simple and reliable isomer specific liquid chromatographic method for the quantitative determination of cefpodoxime proxetil (CP) in rat in situ intestinal perfusate samples. Chromatography was carried out by reversed-phase technique on a C-18 column with a mobile phase composed of 20 mM ammonium acetate buffer (pH 5.0) and acetonitrile in the ratio of 62:38 pumped at a flow-rate of 1 ml/min. The detection was carried out at 235 nm and a column temperature of 30 degrees C. The method was evaluated for the various validation parameters, such as linearity, accuracy, precision, LOD, LOQ, specificity, selectivity, and sample stability. The results of intra- and inter-day validation (n = 3) showed the method to be efficient and the same was applied in an in situ permeability study conducted for CP in rats.
Kakumanu
Vasu Kumar
VK
Department of Pharmaceutical Technology, Formulations, National Institute of Pharmaceutical Education and Research, NIPER, Sector 67, Phase X, SAS Nagar, Punjab 160 062, India.
Arora
Vinod Kumar
VK
Bansal
Arvind Kumar
AK
D000818:Animals; D015296:Ceftizoxime / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D007408:Intestinal Absorption; D007536:Isomerism; D008297:Male; D011355:Prodrugs / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000097627:Cefpodoxime Proxetil
D015296:Ceftizoxime; D011355:Prodrugs; D000097627:Cefpodoxime Proxetil