Enhancement of the binding ability of a ligand for nucleobase recognition by introducing a methyl group.
The recognition ability of pteridine derivatives for nucleobases opposite an abasic (AP) site in an oligodeoxynucleotide (ODN) duplex is enhanced by using a propylene residue (Spacer-C3) as an AP site. The recognition ability is further enhanced both by attaching methyl groups to a fluorescent ligand and by measuring the fluorescence response at 5 degrees C; 6.2 x 10(6) M(-1) of the binding constant is attained between 2-amino-6,7-dimethyl-4-hydroxypteridine and guanine opposite the AP site in water.
Dai
Qing
Q
Department of Chemistry, Graduate School of Science, Tohoku University, Japan.
Xu
Chun-Yan
CY
Sato
Yusuke
Y
Yoshimoto
Keitaro
K
Nishizawa
Seiichi
S
Teramae
Norio
N
D001665:Binding Sites; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D011485:Protein Binding; D011621:Pteridines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014867:Water / Q000737:chemistry
D008024:Ligands; D009705:Nucleosides; D009841:Oligonucleotides; D011621:Pteridines; D014867:Water