Birth after double cryopreservation of human oocytes at metaphase II and pronuclear stages.
OBJECTIVE
To document the possibility to double-cryopreserve human metaphase II and pronuclear oocytes.


METHODS
Case report.


METHODS
University-based IVF center.


METHODS
A couple with primary andrological infertility.


METHODS
Pronuclear stage oocytes derived after sperm injection into frozen-thawed metaphase II oocytes were cryopreserved again for later transfer.


METHODS
Post-thaw survival rates and pregnancy.


RESULTS
Five metaphase II oocytes survived the freeze-thaw procedure. Three of five oocytes presented with two pronuclei after sperm injection and were cryopreserved again. Post-thawing, all three oocytes developed into good quality embryos for transfer. A singleton pregnancy was achieved, resulting in the birth of a healthy girl.


CONCLUSIONS
Double-cryopreservation of oocytes at metaphase II and at pronuclear stage is a treatment option in human-assisted reproduction.
Montag
Markus
M
Department of Gynecological Endocrinology and Reproductive Medicine, University of Bonn, Bonn, Germany. Markus.Montag@ukb.uni-bonn.de
van der Ven
Katrin
K
Dorn
Christoph
C
Isachenko
Vladimir
V
Isachenko
Eugenia
E
van der Ven
Hans
H
D000328:Adult; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008677:Metaphase; D009865:Oocytes / Q000166:cytology*; D036801:Parturition; D011247:Pregnancy; D011418:Prophase; D020554:Sperm Injections, Intracytoplasmic