Searching for markers of Creutzfeldt-Jakob disease in cerebrospinal fluid by two-dimensional mapping.
Differential proteomic analysis has been performed on the cerebrospinal fluid (CSF) of six healthy and six patients suffering form sporadic Creutzfeldt-Jakob disease (sCJD), age- and sex-matched, after immuno-subtraction of albumin and immunoglobulins. These maps have revealed 28 polypeptide chains differentially modulated in the sCJD samples, of which 10 appeared to be up-regulated, the remaining 18 being down-regulated. Among those, 13 could be identified upon digestion and MALDI-TOF, MS analysis. In addition, the strong modulation of cystatin C was also confirmed by immunoblot analysis and the highly altered level of the 14-3-3 proteins that escaped detection by 2-D mapping, could be assessed by Western blots and immuno-detection of monomeric and homo- and hetero-dimeric 14-3-3 isotypes. In search for a panel of potential markers for sCJD, we highlight cystatin C, 14-3-3 proteins, transferrin, ubiquitin, Apoliprotein J and perhaps some of the still unidentified, but strongly modulated polypeptide chains detected in the differential map.
Piubelli
Chiara
C
Department of Neurological and Visual Sciences, Section of Neurology, University of Verona, Verona, Italy.
Fiorini
Michele
M
Zanusso
Gianluigi
G
Milli
Alberto
A
Fasoli
Elisa
E
Monaco
Salvatore
S
Righetti
Pier Giorgio
PG
D048948:14-3-3 Proteins / Q000134:cerebrospinal fluid; D000368:Aged; D016022:Case-Control Studies; D002556:Cerebrospinal Fluid Proteins / Q000378:metabolism*; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid*; D055316:Cystatin C; D015891:Cystatins / Q000134:cerebrospinal fluid; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D008875:Middle Aged; D018620:PrPSc Proteins / Q000134:cerebrospinal fluid; D040901:Proteomics
D048948:14-3-3 Proteins; C525030:CST3 protein, human; D002556:Cerebrospinal Fluid Proteins; D055316:Cystatin C; D015891:Cystatins; D018620:PrPSc Proteins