title: Complex assembly, crystallization and preliminary X-ray crystallographic studies of rhesus macaque MHC Mamu-A*01 complexed with an immunodominant SIV-Gag nonapeptide.
abstract: Simian immunodeficiency virus (SIV) infection in rhesus macaques has been used as the best model for the study of human immunodeficiency virus (HIV) infection in humans, especially in the cytotoxic T-lymphocyte (CTL) response. However, the structure of rhesus macaque (or any other monkey model) major histocompatibility complex class I (MHC I) presenting a specific peptide (the ligand for CTL) has not yet been elucidated. Here, using in vitro refolding, the preparation of the complex of the rhesus macaque MHC I allele (Mamu-A*01) with human beta2m and an immunodominant peptide, CTPYDINQM (Gag_CM9), derived from SIV Gag protein is reported. The complex (45 kDa) was crystallized; the crystal belongs to space group I422, with unit-cell parameters a = b = 183.8, c = 155.2 A. The crystal contains two molecules in the asymmetric unit and diffracts X-rays to 2.8 A resolution. The structure is being solved by molecular replacement and this is the first attempt to determined the crystal structure of a peptide-nonhuman primate MHC complex.
authors.affiliation: Laboratory of Molecular Immunology and Molecular Virology, Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
authors.forename: Fuliang
authors.initials: F
authors.lastname: Chu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lou
authors.forename: Bin
authors.initials: B
authors.lastname: Gao
authors.forename: John I
authors.initials: JI
authors.lastname: Bell
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry*; D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D015683:Gene Products, gag / Q000737:chemistry*; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016056:Immunodominant Epitopes / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002479:Inclusion Bodies; D008253:Macaca mulatta; D010446:Peptide Fragments; D011485:Protein Binding; D015302:Simian Immunodeficiency Virus / Q000737:chemistry*; D014835:Volatilization
chemical_list: D000956:Antigens, Viral; D015683:Gene Products, gag; D015395:Histocompatibility Antigens Class I; D016056:Immunodominant Epitopes; C110764:Mamu-A 01 antigen; D010446:Peptide Fragments