title: Evaluation of genetic damage in human peripheral lymphocytes exposed to mineral trioxide aggregate and Portland cements.
abstract: summary Mineral trioxide aggregate (MTA) and Portland cement are being used in dentistry as root-end-filling material for periapical surgery and for the sealing of communications between the root canal system and the surrounding tissues. However, genotoxicity tests for complete risk assessment of these compounds have not been conducted up to now. In the present study, the genotoxic effects of MTA and Portland cements were evaluated in peripheral lymphocytes from 10 volunteers by the alkaline single cell gel (comet) assay. The results pointed out that the single cell gel (comet) assay failed to detect the presence of DNA damage after a treatment of peripheral lymphocytes by MTA and Portland cements for concentrations up to 1000 mug mL(-1). In summary, our results indicate that exposure to MTA or Portland cements may not be a factor that increases the level of DNA lesions in human peripheral lymphocytes as detected by single cell gel (comet) assay.
authors.affiliation: Departamento de Patologia, Núcleo de Avaliação Toxicogenética e Cancerígena, TOXICAN, Faculdade de Medicina de Botucatu, UNESP, SP, Brazil.
authors.forename: M G
authors.initials: MG
authors.lastname: Braz
authors.forename: E A
authors.initials: EA
authors.lastname: Camargo
authors.forename: D M F
authors.initials: DM
authors.lastname: Salvadori
authors.forename: M E A
authors.initials: ME
authors.lastname: Marques
authors.forename: D A
authors.initials: DA
authors.lastname: Ribeiro
mesh_terms: D000328:Adult; D017607:Aluminum Compounds / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology*; D002478:Cells, Cultured; D020552:Comet Assay / Q000379:methods; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage / Q000235:genetics*; D003738:Dental Cements / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D010087:Oxides / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000494:pharmacology; D017640:Silicates / Q000494:pharmacology*
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D003738:Dental Cements; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D004247:DNA