title: Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: simultaneous binding to DNA phosphate backbone.
abstract: Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Zhao
authors.forename: Qing
authors.initials: Q
authors.lastname: Dai
authors.forename: Takehiro
authors.initials: T
authors.lastname: Seino
authors.forename: Ying-Yu
authors.initials: YY
authors.lastname: Cui
authors.forename: Seiichi
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Norio
authors.initials: N
authors.lastname: Teramae
mesh_terms: D000584:Amiloride / Q000737:chemistry*; D020029:Base Pairing; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D005456:Fluorescent Dyes; D006860:Hydrogen Bonding; D008024:Ligands; D010710:Phosphates / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013941:Thymine / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D010710:Phosphates; D000584:Amiloride; D004247:DNA; D013941:Thymine