Myotube assembly on nanofibrous and micropatterned polymers.
Skeletal muscle consists of parallel bundles of myotubes formed by the fusion of myoblasts. We fabricated nanofibrous and micropatterned polymers as cell culture substrates to guide the morphogenesis of muscular tissue. The nanoscale and microscale topographic features regulate cell and cytoskeleton alignment, myotube assembly, myotube striation, and myoblast proliferation. This bottom-up approach from nanoscale to tissue level demonstrates the potential of nanofibrous polymers for engineering the assembly of cell and tissue structure.
Huang
Ngan F
NF
Joint Graduate Program in Bioengineering, University of California San Francisco/University of California Berkeley, 94720-1762, USA.
Patel
Shyam
S
Thakar
Rahul G
RG
Wu
Jun
J
Hsiao
Benjamin S
BS
Chu
Benjamin
B
Lee
Randall J
RJ
Li
Song
S
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D051379:Mice; D008855:Microscopy, Electron, Scanning; D018485:Muscle Fibers, Skeletal / Q000378:metabolism* / Q000648:ultrastructure; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013379:Substrate Specificity; D023822:Tissue Engineering / Q000379:methods*
D011108:Polymers