title: Mesenchymal lineage precursor cells induce vascular network formation in ischemic myocardium.
abstract: Mesenchymal lineage precursors can be reproducibly isolated from adult mammalian bone marrow and grown in culture. Immunoselection with monoclonal antibodies against STRO-1 and vascular-cell-adhesion molecule 1 (VCAM1/CD106) prior to expansion results in a 1,000-fold enrichment of mesenchymal precursors compared to standard isolation techniques. Intramyocardial injection of human STRO-1-selected precursors in an athymic rat model of acute myocardial infarction results in induction of vascular network formation and arteriogenesis coupled with global functional cardiac recovery.
authors.affiliation: Division of Cardiothoracic Surgery, Columbia University, New York, NY, USA. tpm2102@columbia.edu
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Martens
authors.forename: Fiona
authors.initials: F
authors.lastname: See
authors.forename: Michael D
authors.initials: MD
authors.lastname: Schuster
authors.forename: Hugo P
authors.initials: HP
authors.lastname: Sondermeijer
authors.forename: Marco M
authors.initials: MM
authors.lastname: Hefti
authors.forename: Andrew
authors.initials: A
authors.lastname: Zannettino
authors.forename: Stan
authors.initials: S
authors.lastname: Gronthos
authors.forename: Tetsunori
authors.initials: T
authors.lastname: Seki
authors.forename: Silviu
authors.initials: S
authors.lastname: Itescu
mesh_terms: D036541:ADP-ribosyl Cyclase / Q000032:analysis; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D001854:Bone Marrow Cells / Q000276:immunology; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D003326:Coronary Circulation; D004195:Disease Models, Animal; D058851:GPI-Linked Proteins; D006439:Hemodynamics; D006801:Humans; D018189:Immunomagnetic Separation; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D017202:Myocardial Ischemia / Q000503:physiopathology / Q000628:therapy*; D018919:Neovascularization, Physiologic; D051381:Rats; D011923:Rats, Nude; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis
chemical_list: D015703:Antigens, CD; D058851:GPI-Linked Proteins; D019010:Vascular Cell Adhesion Molecule-1; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2