title: X-ray diffraction analysis and molecular-replacement solution of the cyan fluorescent protein dsFP483.
abstract: A novel cyan fluorescent protein, dsFP483 from the coral Discosoma striata, has been crystallized. Diffraction data were collected to 2.1 A and processed in space group C2. Molecular-replacement methods were applied using the closely related red fluorescent protein DsRed as a search model. The asymmetric unit appears to contain six protein molecules (1.5 tetramers), five of which (83%) could be located by the molecular-replacement searches.
authors.affiliation: Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA.
authors.forename: Meitian
authors.initials: M
authors.lastname: Wang
authors.forename: Hetal N
authors.initials: HN
authors.lastname: Patel
authors.forename: Rebekka M
authors.initials: RM
authors.lastname: Wachter
mesh_terms: D000818:Animals; D037421:Anthozoa; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008164:Luminescent Proteins / Q000494:pharmacology; D008958:Models, Molecular; D015233:Models, Statistical; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: C485182:Cyan Fluorescent Protein; D008164:Luminescent Proteins; C413663:fluorescent protein 583; D049452:Green Fluorescent Proteins