title: Kitasatospora sampliensis sp. nov., a novel actinobacterium isolated from soil of a sugar-cane field in India.
abstract: Polyphasic characterization of an actinomycete strain VT-36(T) isolated from a sugar-cane field soil sample collected in Punjab State, India, revealed that the strain belongs to the genus Kitasatospora. The strain's chemotaxonomic characters and G+C content of DNA (76.5 mol%) were typical of members of the genus. Analysis of the 16S rRNA gene sequence supported the generic affiliation of the strain and showed that its closest phylogenetic relative was Kitasatospora putterlickiae F18-98T (= DSM 44665T) (98.3 % 16S rRNA gene sequence similarity). The similarities with type strains of all other Kitasatospora species were in the range 95.1-97.0 %. The results of DNA-DNA hybridization showed 54 % relatedness of the isolate and K. putterlickiae F18-98T. Based on the above data and the phenotypic differences from K. putterlickiae and other Kitasatospora species, it is proposed that the isolate should be classified as the type strain of a novel species, Kitasatospora sampliensis sp. nov., with strain VT-36T (= MTCC 6546T = DSM 44898T = JCM 13010T) as the type strain.
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector 39-A, Chandigarh, 160 036, India.
authors.forename: S
authors.initials: S
authors.lastname: Mayilraj
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector 39-A, Chandigarh, 160 036, India.
authors.forename: S
authors.initials: S
authors.lastname: Krishnamurthi
authors.affiliation: Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector 39-A, Chandigarh, 160 036, India.
authors.forename: P
authors.initials: P
authors.lastname: Saha
authors.affiliation: Department of Microbiology, Guru Nanak Dev University (GNDU), Amritsar, 143 005, India.
authors.forename: H S
authors.initials: HS
authors.lastname: Saini
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D002241:Carbohydrates; D004275:DNA, Ribosomal / Q000737:chemistry; D007194:India; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D002241:Carbohydrates; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S