title: [Construction and identification of cDNA library from peripheral blood lymphocytes of Tibetans in Qinghai-Tibet plateau].
abstract: OBJECTIVE
  To construct the full length cDNA library of the peripheral blood lymphocytes in Tibetans and to identify the quality of the library.
  
  
  METHODS
  The lymphocytes were separated from healthy Tibetan fresh blood, and total RNA was extracted. The full length cDNA library was constructed by SMART (switching mechanism at 5' end of RNA transcript) technique. The unamplified library was titered, and the percentage of recombinant clones were determined. The library was amplified, and the size of the inserts was identified by PCR.
  
  
  RESULTS
  The titer of the constructed cDNA library was 1.8x10(9) pfu/L with the percentage of recombinant clones above 98%, and that of the amplified library was 8x10(12) pfu/L. The insert's size ranged from 0.75 to 2.0 kb with the average length of 1.35 kb.
  
  
  CONCLUSIONS
  The constructed cDNA library of peripheral blood lymphocytes of Tibetans has excellent quality, and it lays a solid founclation for further screening and cloning hypoxia-related genes of Tibetans living at high altitudes.
authors.affiliation: Research Center for High Altitude Medicine of Qinghai University, Xining 810001, China. woshiyuntian@163.com
authors.forename: Yun-tian
authors.initials: YT
authors.lastname: Chen
authors.forename: Jian-fang
authors.initials: JF
authors.lastname: Wu
authors.forename: Yue
authors.initials: Y
authors.lastname: Cao
authors.forename: Ri-li
authors.initials: RL
authors.lastname: Ge
mesh_terms: D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D004274:DNA, Recombinant / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008913:Minority Groups; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D018609:Tibet / Q000208:ethnology; D055815:Young Adult
chemical_list: D004274:DNA, Recombinant