title: [Correlation of changes in circulating bone marrow stem cells in pregnant rabbits after acute myocardial infarction with serum estradiol].
abstract: OBJECTIVE
  To investigate the changes in circulating bone marrow stem cells in pregnant rabbits after AMI (AMI) and their relationship with estradiol.
  
  
  METHODS
  Three groups of rabbits were used, namely pregnancy and AMI group, AMI group without pregnancy, and sham operation group with pregnancy. The ratio of CD90(+) cells in the peripheral blood was determined with flow cytometry in all the rabbits, and serum estradiol level measured. Four weeks after AMI, hemodynamic measurements were carried out. The morphological changes of the myocardial tissues were examined with ImageJ 1.31.
  
  
  CONCLUSIONS
  Four weeks after AMI, the two pregnancy groups showed a higher Left ventricular end systolic pressure(LVESP) and+dp/dtmax, lower left ventricular end-diastolic pressure (LVEDP) and -dp/dtmax and high levels of CD90(+) cells in peripheral blood than AMI group without pregnancy (P<0.01). The ratio of circulating CD90(+) cells increased gradually with gestational age and peaked at the end stage of pregnancy. After delivery the circulating CD90+ cell ratio decreased sharply, showing a significant correlation with serum estradiol level (r=0.725, P<0.01). Four weeks after AMI, the pregnancy group had smaller myocardial infarction (MI) volume than the non-pregnant group (22.17+/-6.34% vs 38.86+/-5.97%, P<0.05). Circulating bone marrow stem cells increased during pregnancy with gestational age and peaked at the end stage of pregnancy. Ten days after delivery, the stem cells resumed basically the normal level. The proportion of circulating bone marrow stem cells was significantly correlated with the level of serum estradiol during pregnancy, and mobilization of the bone marrow stem cells induced by acute ischemic event in pregnant rabbits was advanced. 4 weeks after AMI, the pregnant rabbits showed better heart contraction and diastolic function than the non-pregnant ones.
authors.affiliation: Department of Cardiology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China. zhengxp001@160.com
authors.forename: Xiao-pu
authors.initials: XP
authors.lastname: Zheng
authors.forename: Ai-qun
authors.initials: AQ
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: An-ping
authors.initials: AP
authors.lastname: Dong
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Geng
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D004958:Estradiol / Q000097:blood*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D009200:Myocardial Contraction; D009203:Myocardial Infarction / Q000097:blood* / Q000503:physiopathology; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000097:blood; D011817:Rabbits; D018800:Thy-1 Antigens / Q000097:blood*; D013997:Time Factors
chemical_list: D018800:Thy-1 Antigens; D004958:Estradiol