Cysteine-free glutathione-S-tranferase as a tool for thiol-specific labeling of proteins.
Tzvetkov
Nikolay
N
Max-Planck Institute of Biochemistry, Hanover Medical School, Martinsried, Germany. tzvetkov@bpc.mh-hannover.de
Breuer
Peter
P
Boteva
Raina
R
D019943:Amino Acid Substitution; D002850:Chromatography, Gel; D002942:Circular Dichroism; D003545:Cysteine / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010957:Plasmids; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012694:Serine / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D011506:Proteins; D013438:Sulfhydryl Compounds; D012694:Serine; D005982:Glutathione Transferase; D005978:Glutathione; D003545:Cysteine