title: Effects of anti-bursin monoclonal antibody on immunosuppression in the duck (Cherry Valley duck).
abstract: To study the immunologic function of bursin, we analyzed the effects of anti-bursin monoclonal antibody (mAb) on the immunosuppression in ducks (Cherry Valley duck) by injecting various doses of the anti-bursin mAb into 13-d duck embryos. After hatch, cell-mediated immune activity and humoral responses were studied using lymphocyte proliferation test, tube agglutination test, and indirect enzyme-linked immunosorbent assay to detect anti-Escherichia coli antibodies and antibodies to Riemerella anatipestifer, respectively. Simultaneously, relative weights (BW-adjusted) of bursa of Fabricius (BF), spleen, and thymus were determined. Additionally, the morphology of BF, spleen, and thymus was examined at various ages using conventional histology. Follicle morphology of BF was analyzed by image analysis. The results indicated that anti-bursin mAb markedly decreased duck lymphocyte proliferation, the antibody-producing ability to bacteria, as well as the relative BF weight. Moreover, the anti-bursin mAb hindered the development of BF follicles.
authors.affiliation: Institute of Hydrobiology of Chinese Academy of Sciences, WuHan, China. gsl@jmu.edu.cn
authors.forename: S
authors.initials: S
authors.lastname: Guo
authors.forename: N H
authors.initials: NH
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Guan
authors.forename: J
authors.initials: J
authors.lastname: Feng
authors.forename: W
authors.initials: W
authors.lastname: Huang
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D002060:Bursa of Fabricius / Q000033:anatomy & histology; D004372:Ducks / Q000196:embryology / Q000276:immunology*; D004625:Embryo, Nonmammalian / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D041965:Flavobacteriaceae / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007108:Immune Tolerance / Q000276:immunology*; D007114:Immunization / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation / Q000187:drug effects; D008221:Lymphoid Tissue / Q000033:anatomy & histology; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000276:immunology* / Q000502:physiology; D009929:Organ Size / Q000187:drug effects
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007106:Immune Sera; D007074:Immunoglobulin G; D009842:Oligopeptides; C016281:bursopoietin