Identification of alternatively spliced dab1 isoforms in zebrafish.
We have investigated the genomic organization, the occurrence of alternative splicing and the differential expression of the zebrafish disabled1 (dab1) gene. Dab1 is a key effector of the Reelin pathway, which regulates neuronal migration during brain development in vertebrates. The coding region of the zebrafish dab1 gene spans over 600 kb of genomic DNA and is composed of 15 exons. Alternative splicing in a region enriched for tyrosine residues generates at least three different isoforms. These isoforms are developmentally regulated and show differential tissue expression. Comparison with mouse and human data shows an overall conservation of the genomic organization with different alternative splicing events generating species-specific isoforms. Because these alternative splicing events give rise to isoforms with different numbers of phosphorylateable tyrosines, we speculate that alternative splicing of the dab1 gene in zebrafish and in other vertebrates regulates the nature of the cellular response to the Reelin signal.
Costagli
Arianna
A
Department of Anatomy and Developmental Biology, University College London, Gower Street, London, WC1E 6BT, UK.
Felice
Barbara
B
Guffanti
Alessandro
A
Wilson
Stephen W
SW
Mione
Marina
M
D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D018387:Codon, Initiator; D018388:Codon, Terminator; D018076:DNA, Complementary; D005091:Exons; D005786:Gene Expression Regulation; D006801:Humans; D017403:In Situ Hybridization; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D009474:Neurons / Q000502:physiology; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D000091042:Reelin Protein; D013045:Species Specificity; D014443:Tyrosine / Q000235:genetics; D015027:Zebrafish / Q000235:genetics*
D048868:Adaptor Proteins, Signal Transducing; D018387:Codon, Initiator; D018388:Codon, Terminator; C115785:DAB1 protein, human; D018076:DNA, Complementary; C103773:Dab1 protein, mouse; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D000091042:Reelin Protein; D014443:Tyrosine; C000717127:RELN protein, human; C000717128:Reln protein, mouse