title: Expression of the onconeural CV2/CRMP5 antigen in thymus and thymoma.
abstract: Anti-CV2 antibodies (AB) react with the developmentally regulated neural proteins CRMPs and particularly with CRMP5. They occur with small cell lung cancer (SCLC) and thymoma. SCLCs universally express CRMP5. We investigated the expression of CRMPs in thymoma and thymus. In thymoma, none of the CRMPs were detected by immunohistochemistry in tumorous epithelial cells with specific antibodies including CRMP5 but an antibody reacting with a peptide common to the CRMPs labeled a 66-kDa protein in Western blot of rat brain, thymus, and thymoma extracts. Thus, the normal CRMP5 is probably not expressed by tumorous epithelial cells. These results indicate that the mechanisms leading to CRMP5 autoimmunization are different in SCLC and thymoma.
authors.affiliation: Equipe d'Accueil 3062, Université Jean-Monnet, Saint-Etienne, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Camdessanché
authors.forename: François
authors.initials: F
authors.lastname: Lassablière
authors.forename: David
authors.initials: D
authors.lastname: Meyronnet
authors.forename: Karine
authors.initials: K
authors.lastname: Férraud
authors.forename: Léna
authors.initials: L
authors.lastname: Absi
authors.forename: Jérôme
authors.initials: J
authors.lastname: Honnorat
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Antoine
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D002531:Cerebellum / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D006867:Hydrolases; D007150:Immunohistochemistry / Q000379:methods; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013945:Thymoma / Q000235:genetics / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism*; D013952:Thymus Hyperplasia / Q000235:genetics / Q000473:pathology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C413755:Dpysl5 protein, rat; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C415087:DPYSL5 protein, human; D006867:Hydrolases