title: Preliminary crystallographic analysis of sugar cane phosphoribosylpyrophosphate synthase.
abstract: Phosphoribosylpyrophosphate synthases (PRS; EC 2.7.6.1) are enzymes that are of central importance in several metabolic pathways in all cells. The sugar cane PRS enzyme contains 328 amino acids with a molecular weight of 36.6 kDa and represents the first plant PRS to be crystallized, as well as the first phosphate-independent PRS to be studied in molecular detail. Sugar cane PRS was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. Using X-ray diffraction experiments it was determined that the crystals belong to the orthorhombic system, with space group P2(1)2(1)2 and unit-cell parameters a = 213.2, b = 152.6, c = 149.3 A. The crystals diffract to a maximum resolution of 3.3 A and a complete data set to 3.5 A resolution was collected and analysed.
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, Caixa Postal 369, 13560-970 São Carlos, SP, Brazil.
authors.forename: H B
authors.initials: HB
authors.lastname: Napolitano
authors.forename: S A
authors.initials: SA
authors.lastname: Sculaccio
authors.forename: O H
authors.initials: OH
authors.lastname: Thiemann
authors.forename: G
authors.initials: G
authors.lastname: Oliva
mesh_terms: D004926:Escherichia coli / Q000201:enzymology; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D012268:Ribose-Phosphate Pyrophosphokinase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D031786:Saccharum / Q000201:enzymology*; D014162:Transfection; D014961:X-Ray Diffraction
chemical_list: D011994:Recombinant Proteins; D012268:Ribose-Phosphate Pyrophosphokinase