Crystallization and preliminary X-ray crystallographic study of disproportionating enzyme from potato.
Disproportionating enzyme (D-enzyme; EC 2.4.1.25) is a 59 kDa protein that belongs to the alpha-amylase family. D-enzyme catalyses intramolecular and intermolecular transglycosylation reactions of alpha-1,4 glucan. A crystal of the D-enzyme from potato was obtained by the hanging-drop vapour-diffusion method. Preliminary X-ray data showed that the crystal diffracts to 2.0 A resolution and belongs to space group C222(1), with unit-cell parameters a = 69.7, b = 120.3, c = 174.2 A.
Imamura
Kayo
K
Laboratory of Enzyme Chemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
Matsuura
Takanori
T
Ye
Zhengmao
Z
Takaha
Takeshi
T
Fujii
Kazutoshi
K
Kusunoki
Masami
M
Nitta
Yasunori
Y
D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D006004:Glycogen Debranching Enzyme System / Q000737:chemistry* / Q000302:isolation & purification; D006031:Glycosylation; D010940:Plant Proteins / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000201:enzymology*; D014961:X-Ray Diffraction
D017931:DNA Primers; D006004:Glycogen Debranching Enzyme System; D010940:Plant Proteins; D011994:Recombinant Proteins; C020569:4 alpha-glucanotransferase