Investigation of pseudorabies virus latency in nervous tissues of seropositive pigs exposed to field strain.
The prevalence and quantity of latent pseudorabies virus (PrV) in nervous tissues of pigs exposed to field strain in Korea was investigated by nested and real-time PCR. Nervous tissues including trigeminal ganglion (TG), olfactory bulb (OB), and brain stem (BS) were collected from 94 seropositive pigs. PrV latent infection in nervous tissues was initially investigated by nested PCR targeting three glycoprotein genes (gB, gE, and gG). Based on the obtained result, latent infection was detected in 95.7% of screened animals. Furthermore, it was revealed that the examined tissues harbored different copy numbers of latent PrV genome ranging from <10(2.0) to 10(7.1) copies per microgram of genomic DNA in real-time PCR analysis. These results show that under normal conditions, levels of latent PrV in the nervous tissues of pigs can vary across a wide range. Therefore, the data presented here provides information regarding control of the endemic state of PrV in Korea.
Yoon
Hyun-A
HA
Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Eo
Seong-Kug
SK
Aleyas
Abi George
AG
Cha
Se-Yeoun
SY
Lee
John-Hwa
JH
Chae
Joon-Seok
JS
Jang
Hyung-Kwan
HK
Cho
Eong-Gon
EG
Song
Hee-Jong
HJ
D000818:Animals; D001933:Brain Stem / Q000821:virology; D002353:Carrier State / Q000662:veterinary / Q000821:virology; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D005260:Female; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D007723:Korea / Q000453:epidemiology; D009417:Nerve Tissue / Q000821:virology*; D009830:Olfactory Bulb / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011557:Pseudorabies / Q000453:epidemiology / Q000276:immunology / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000276:immunology / Q000821:virology*; D012668:Trigeminal Ganglion / Q000821:virology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics; D017735:Virus Latency
D004279:DNA, Viral; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins