title: Transformation of undomesticated strains of Bacillus subtilis by protoplast electroporation.
abstract: A rapid method combining the use of protoplasts and electroporation was developed to transform recalcitrant wild strains of Bacillus subtilis. The method described here allows transformation with both replicative and integrative plasmids, as well as with chromosomal DNA, and provides a valuable tool for molecular genetic analysis of interesting Bacillus strains, which are hard to transform by conventional methods.
authors.affiliation: Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos s/n, E-29071 Málaga, Spain.
authors.forename: Diego
authors.initials: D
authors.lastname: Romero
authors.forename: Alejandro
authors.initials: A
authors.lastname: Pérez-García
authors.forename: Jan-Willem
authors.initials: JW
authors.lastname: Veening
authors.forename: Antonio
authors.initials: A
authors.lastname: de Vicente
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology*; D018274:Electroporation / Q000379:methods*; D011523:Protoplasts / Q000502:physiology*; D014170:Transformation, Genetic / Q000502:physiology*