Generation of new human embryonic stem cell lines with diploid and triploid karyotypes.
Human pluripotent embryonic stem cells (hESC) have great promise for research into human developmental biology and the development of cell therapies for the treatment of diseases. To meet the increased demand for characterized hESC lines, we present the derivation and characterization of five hESC lines on mouse embryonic fibroblast cells. Our stem cell lines are characterized by morphology, long-term expansion, and expression profiles of a number of specific markers, including TRA-1-60, TRA-1-81, alkaline phosphatase, connexin 43, OCT-4, NANOG, CXCR4, NODAL, LEFTY2, THY-1, TDGF1, PAX6, FOXD3, SOX2, EPHA2, FGF4, TAL1, AC133 and REX-1. The pluripotency of the cell line was confirmed by spontaneous differentiation under in vitro conditions. Whereas all of the cell lines expressed all the characteristics of undifferentiated pluripotent hESC, two of the cell lines carried a triploid karyotype.
Baharvand
Hossein
H
Department of Stem Cells, Royan Institute, Tehran, Iran. baharvand50@yahoo.com
Ashtiani
Saeid Kazemi
SK
Taee
Adeleh
A
Massumi
Mohammad
M
Valojerdi
Mojtaba R
MR
Yazdi
Poopak E
PE
Moradi
Shabnam Z
SZ
Farrokhi
Ali
A
D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D004171:Diploidy; D046149:Embryo Culture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007621:Karyotyping; D011123:Polyploidy
D015415:Biomarkers