title: [Identification of sarcosaphagous flies by analyses the sequence of 16S rDNA].
abstract: OBJECTIVE
  To solve the difficulties of identification of Sarcosaphagous flies such as Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) which could not be identified by analyzing the 278bp and 635 bp regions of the gene encoding for cytochrome oxidase subunit I and II (CO I and CO II) in mtDNA.
  
  
  METHODS
  Specimens were collected from the corpses of rabbits on the grassland in Huhhot and Chengdu, the sequences of 551 bp region of 16S rDNA of their mtDNA were analyzed, the multiple-alignment program DNAMAN(version 4.0) and MEGA 2.1 sofeware were employed for sequence alignments neighbour-joining tree construction.
  
  
  RESULTS
  Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) were distinguished successfully by sequence analysis of The 551 bp region of the gene of 16S rDNA.
  
  
  CONCLUSIONS
  The 551 bp region of the gene of 16S rDNA of sarcosaphagous flies can be used for identifying them on species level effectively. It is likely to be a successful compliment to identify the sarcosaphagous flies by sequence analysis of CO I and CO II in mtDNA.
authors.affiliation: Institute of Forensic Medicine, Sichuan University, Chengdu 610041, China. lupusstar@163.com
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Sun
authors.forename: Ji-feng
authors.initials: JF
authors.lastname: Cai
authors.forename: Bin-wu
authors.initials: BW
authors.lastname: Ying
authors.forename: Yue-qin
authors.initials: YQ
authors.lastname: Fang
authors.forename: Li-bing
authors.initials: LB
authors.lastname: Yun
authors.forename: Wan-an
authors.initials: WA
authors.lastname: Yuan
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Hou
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D004175:Diptera / Q000145:classification / Q000235:genetics*; D005554:Forensic Medicine / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D011817:Rabbits; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S