Ultra-short echo time spectroscopic imaging in rats: implications for monitoring lipids in glioma gene therapy.
We demonstrate the feasibility of using ultra-short echo time (TE = 2 ms) magnetic resonance spectroscopic imaging (MRSI) to detect intracranial mobile lipids in the rat brain. High-performance outer volume suppression and pre-localization were demonstrated in phantoms and by the total absence of signals arising from extra-cranial lipids in MRSI spectra from control rats. The sequence performance was tested on glioma-bearing BDIX rats. Fast-relaxing lipid signals were spatially varied within a glioma during herpes simplex virus thymidine kinase-mediated gene therapy, demonstrating the potential application of this method.
Liimatainen
Timo
T
Department of Biomedical NMR, A.I.Virtanen Institute, University of Kuopio, Kuopio, Finland.
Hakumäki
Juhana
J
Tkác
Ivan
I
Gröhn
Olli
O
D000818:Animals; D005910:Glioma / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D008055:Lipids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D008055:Lipids