Molecular genetics: DNA analysis of a putative dog clone.
In August 2005, Lee et al. reported the first cloning of a domestic dog from adult somatic cells. This putative dog clone was the result of somatic-cell nuclear transfer from a fibroblast cell of a three-year-old male Afghan hound into a donor oocyte provided by a dog of mixed breed. In light of recent concerns regarding the creation of cloned human cell lines from the same institution, we have undertaken an independent test to determine the validity of the claims made by Lee et al..
Parker
Heidi G
HG
Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Kruglyak
Leonid
L
Ostrander
Elaine A
EA
D000346:Afghanistan; D000483:Alleles; D000818:Animals; D000829:Animals, Domestic / Q000145:classification / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics; D019976:Cloning, Organism / Q000379:methods / Q000592:standards* / Q000662:veterinary*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004285:Dogs / Q000145:classification / Q000235:genetics*; D005260:Female; D005347:Fibroblasts; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D007723:Korea; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D053652:Nuclear Transfer Techniques; D010375:Pedigree; D011110:Polymorphism, Genetic / Q000235:genetics; D011247:Pregnancy; D011336:Probability; D015203:Reproducibility of Results; D014481:United States
D004272:DNA, Mitochondrial; D005819:Genetic Markers; D004247:DNA