Transit time dispersion in pulmonary and systemic circulation: effects of cardiac output and solute diffusivity.
We present an in vivo method for analyzing the distribution kinetics of physiological markers into their respective distribution volumes utilizing information provided by the relative dispersion of transit times. Arterial concentration-time curves of markers of the vascular space [indocyanine green (ICG)], extracellular fluid (inulin), and total body water (antipyrine) measured in awake dogs under control conditions and during phenylephrine or isoproterenol infusion were analyzed by a recirculatory model to estimate the relative dispersions of transit times across the systemic and pulmonary circulation. The transit time dispersion in the systemic circulation was used to calculate the whole body distribution clearance, and an interpretation is given in terms of a lumped organ model of blood-tissue exchange. As predicted by theory, this relative dispersion increased linearly with cardiac output, with a slope that was inversely related to solute diffusivity. The relative dispersion of the flow-limited indicator antipyrine exceeded that of ICG (as a measure of intravascular mixing) only slightly and was consistent with a diffusional equilibration time in the extravascular space of approximately 10 min, except during phenylephrine infusion, which led to an anomalously high relative dispersion. A change in cardiac output did not alter the heterogeneity of capillary transit times of ICG. The results support the view that the relative dispersions of transit times in the systemic and pulmonary circulation estimated from solute disposition data in vivo are useful measures of whole body distribution kinetics of indicators and endogenous substances. This is the first model that explains the effect of flow and capillary permeability on whole body distribution of solutes without assuming well-mixed compartments.
Weiss
Michael
M
Section of Pharmacokinetics, Dept. of Pharmacology, Martin Luther Univ. Halle-Wittenberg, D-06097 Halle, Germany. michael.weiss@medizin.uni-halle.de
Krejcie
Tom C
TC
Avram
Michael J
MJ
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000465:Algorithms; D000818:Animals; D000983:Antipyrine / Q000493:pharmacokinetics; D001775:Blood Circulation / Q000502:physiology*; D002302:Cardiac Output / Q000502:physiology*; D004058:Diffusion; D004285:Dogs; D006614:Hindlimb / Q000098:blood supply; D007208:Indocyanine Green; D007328:Insulin; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008954:Models, Biological; D015233:Models, Statistical; D010656:Phenylephrine / Q000494:pharmacology; D011652:Pulmonary Circulation / Q000502:physiology*; D012039:Regional Blood Flow / Q000502:physiology; D014662:Vasoconstrictor Agents / Q000494:pharmacology
D000318:Adrenergic beta-Agonists; D007328:Insulin; D014662:Vasoconstrictor Agents; D010656:Phenylephrine; D007208:Indocyanine Green; D007545:Isoproterenol; D000983:Antipyrine