title: Effects of PPAR and RXR ligands in semaphorin 6B gene expression of human MCF-7 breast cancer cells.
abstract: This study tests the hypothesis that the activators of peroxisome proliferator-activated receptors (PPARs) and 9-cis-retinoic acid receptor (RXR) regulate human semaphorin 6B (Sema6B) gene expression. The human MCF-7 breast adenocarcinoma cell line was chosen because it expresses Sema6B at a high level. The Sema6B mRNA level was analyzed by RT-PCR and the semaphorin 6B protein content was determined using a polyclonal antibody that we have produced and characterized. Treatments with fenofibrate (a PPARalpha activator) and troglitazone (a PPARgamma ligand) strongly decreased the Sema6B mRNA. The drop in Sema6B mRNA level and in protein content was more important when the treatment combined the action of fenofibrate or troglitazone and 9-cis-retinoic acid. On the other hand, no significant change was observed in the Sema6B mRNA and protein levels when the cells were exposed to the combined action of GW610742 (a PPARbeta activator) and 9-cis-retinoic acid. These data suggest that PPARalpha/RXR and PPARgamma/RXR heterodimers are involved in the regulation of Sema6B gene expression and open new perspectives concerning the participation of these nuclear receptors in cell recognition and migration.
authors.affiliation: IFR 111/EA 3446, Peroxisome Proliferators, Faculty of Sciences, BP 239, 54506 Vandoeuvre-les-Nancy, France. Hossam.Murad@scbiol.uhp-nancy.fr
authors.forename: Hossam
authors.initials: H
authors.lastname: Murad
authors.forename: Philippe
authors.initials: P
authors.lastname: Collet
authors.forename: Cecile
authors.initials: C
authors.lastname: Huin-Schohn
authors.forename: Nehmann
authors.initials: N
authors.lastname: Al-Makdissy
authors.forename: Geraldine
authors.initials: G
authors.lastname: Kerjan
authors.forename: Alain
authors.initials: A
authors.lastname: Chedotal
authors.forename: Mireille
authors.initials: M
authors.lastname: Donner
authors.forename: Marie Dominique
authors.initials: MD
authors.lastname: Devignes
authors.forename: Philippe
authors.initials: P
authors.lastname: Becuwe
authors.forename: Herve
authors.initials: H
authors.lastname: Schohn
authors.forename: Lionel
authors.initials: L
authors.lastname: Domenjoud
authors.forename: Michel
authors.initials: M
authors.lastname: Dauça
mesh_terms: D006801:Humans; D000077556:Alitretinoin; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D002839:Chromans / Q000494:pharmacology; D019281:Dimerization; D011345:Fenofibrate / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D019073:HT29 Cells; D020014:K562 Cells; D047493:PPAR alpha / Q000819:agonists / Q000737:chemistry; D047495:PPAR gamma / Q000819:agonists / Q000737:chemistry; D047488:Retinoid X Receptors / Q000819:agonists / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism; D013844:Thiazoles / Q000494:pharmacology; D045162:Thiazolidinediones / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D000077288:Troglitazone
chemical_list: D000077556:Alitretinoin; D002839:Chromans; D011345:Fenofibrate; C503394:GW 610742; D047493:PPAR alpha; D047495:PPAR gamma; D047488:Retinoid X Receptors; D012333:RNA, Messenger; C504081:SEMA6B protein, human; D039961:Semaphorins; D013844:Thiazoles; D045162:Thiazolidinediones; D014212:Tretinoin; D000077288:Troglitazone