Novel immunoassay for carcinoembryonic antigen based on protein A-conjugated immunosensor chip by surface plasmon resonance and cyclic voltammetry.
In this study, an immunosensor chip utilizing surface plasmon resonance (SPR) and cyclic voltammetry (CV) was fabricated for detecting carcinoembryonic antigen (CEA). Specifically, we applied in parallel an SPR instrument and a CV device to monitor the assembly of carcinoembryonic antibody (anti-CEA) on a protein A-conjugated surface and the subsequent ligand reaction. The immunosensor chips were constructed by various concentrations of protein A. To determine the surface characteristics of different self-assembly monolayers (SAMs), several quantitative and kinetic measurements were carried out. The extent of immobilization of anti-CEA and the immune response of anti-CEA antibody against CEA were measured using the SPR instrument and CV device. The terminal functional groups of protein A have different effects on the adsorption and covalent binding of immunoprotein depending on the steric hindrance. Through the parallel measurements, we demonstrate that SPR and CV are sensitive to measure the antigen-antibody binding capacity.
Tang
Dian-Ping
DP
Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest University, 400700, Chongqing, People's Republic of China. tdping@swnu.edu.cn
Yuan
Ruo
R
Chai
Ya-Qin
YQ
D015374:Biosensing Techniques / Q000295:instrumentation*; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000276:immunology; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D013205:Staphylococcal Protein A / Q000032:analysis* / Q000276:immunology; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D014159:Transducers
D002272:Carcinoembryonic Antigen; D013205:Staphylococcal Protein A