An auxin-inducible F-box protein CEGENDUO negatively regulates auxin-mediated lateral root formation in Arabidopsis.
Previously, we characterized 92 Arabidopsis genes (AtSFLs) similar to the S-locus F-box genes involved in S-RNase-based self-incompatibility and found that they likely play diverse roles in Arabidopsis. In this study, we investigated the role of one of these genes, CEGENDUO (CEG, AtSFL61), in the lateral root formation. A T-DNA insertion in CEG led to an increased lateral root production, which was complemented by transformation of the wild-type gene. Its downregulation by RNAi also produced more lateral roots in transformed Arabidopsis plants whereas its overexpression generated less lateral roots compared to wild-type, indicating that CEG acts as a negative regulator for the lateral root formation. It was found that CEG was expressed abundantly in vascular tissues of the primary root, but not in newly formed lateral root primordia and the root meristem, and induced by exogenous auxin NAA (alpha-naphthalene acetic acid). In addition, the ceg mutant was hyposensitive to NAA, IAA (indole-3-acetic acid) and 2,4-D (2,4-dichlorophenoxyacetic acid), as well as the auxin transport inhibitor TIBA (3,3,5-triiodobenzoic acid), showing that CEG is an auxin-inducible gene. Taken together, our results show that CEG is a novel F-box protein negatively regulating the auxin-mediated lateral root formation in Arabidopsis.
Dong
Li
L
Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, Beijing 100080, China.
Wang
Lei
L
Zhang
Yu'e
Y
Zhang
Yansheng
Y
Deng
Xingwang
X
Xue
Yongbiao
Y
D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics; D005816:Genetic Complementation Test; D007210:Indoleacetic Acids / Q000494:pharmacology*; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D030821:Plants, Genetically Modified / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D014283:Triiodobenzoic Acids / Q000494:pharmacology
D029681:Arabidopsis Proteins; C510157:CEGENDUO protein, Arabidopsis; D044783:F-Box Proteins; D007210:Indoleacetic Acids; D012333:RNA, Messenger; D012097:Repressor Proteins; D014283:Triiodobenzoic Acids; C052968:2,3,5-triiodobenzoic acid