title: Immune interaction between components of acellular pertussis-diphtheria-tetanus (DTaP) vaccine and Haemophilus influenzae b (Hib) conjugate vaccine in a rat model.
abstract: We have previously shown that, consistent with clinical trial results, the immune response to a Haemophilus influenzae b (Hib) conjugate vaccine in a rat model was compromised and modulated when given combined with a DTaP3 vaccine, as compared to both vaccines given separately. The present study extended our investigation to evaluate the immunogenicity of all DTaP3 components in combined versus separate administration of Hib with DTaP3 and investigated immune interactions between Hib and individual components of DTaP3. Rats were immunised with Hib and DTaP3 or with Hib and individual DTaP3 components. Cellular and humoral immune responses to Hib and DTaP3 components were evaluated. Our results indicate that the immunogenicity of DTaP3 components was similar or greater in combined versus separate administration of Hib and DTaP3. Moreover, combined administration of Hib and TT reduced immunogenicity of both Hib and TT. Hib immunogenicity was also significantly reduced when given combined with FHA and following adsorption to Al(OH)3.
authors.affiliation: Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK. fmawas@nibsc.ac.uk
authors.forename: Fatme
authors.initials: F
authors.lastname: Mawas
authors.forename: Robert
authors.initials: R
authors.lastname: Dickinson
authors.forename: Alexandra
authors.initials: A
authors.lastname: Douglas-Bardsley
authors.forename: Dorothy K L
authors.initials: DK
authors.lastname: Xing
authors.forename: Dorothea
authors.initials: D
authors.lastname: Sesardic
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Corbel
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology; D000276:Adjuvants, Immunologic / Q000494:pharmacology; D017607:Aluminum Compounds / Q000494:pharmacology; D000536:Aluminum Hydroxide / Q000494:pharmacology; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D016667:Bacterial Capsules; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D018073:Haemophilus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D010710:Phosphates / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013745:Tetanus Toxoid / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000276:Adjuvants, Immunologic; D017607:Aluminum Compounds; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D013745:Tetanus Toxoid; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; D015847:Interleukin-4; D000536:Aluminum Hydroxide; D007371:Interferon-gamma; C012714:aluminum phosphate