title: Microcantilevers modified by horseradish peroxidase intercalated nano-assembly for hydrogen peroxide detection.
abstract: Detection of hydrogen peroxide is of industry interest and of biological importance. Here we report a new approach to hydrogen peroxide measurement using multilayer modified microcantilevers. Through a layer-by-layer nanoassembly technique, horseradish peroxidase was intercalated into a nanoscale multilayer assembly on one surface of microcantilevers. These enzyme-functionalized microcantilevers deflected in response to hydrogen peroxide concentrations in the nanomolar level. The magnitudes of bending were proportional to the concentrations of hydrogen peroxide. Furthermore, our study also showed that microcantilever technique may be used as a novel, more sensitive tool for the study of the conformational or structural changes of enzymes or proteins on surfaces.
authors.affiliation: Institute for Micromanufacturing, Louisiana Tech University, 911 Hergot Avenue, Ruston, LA 71272, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Yan
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Shi
authors.forename: Kalisha
authors.initials: K
authors.lastname: Hill
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Ji
mesh_terms: D000327:Adsorption; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004798:Enzymes / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D008956:Models, Chemical; D008968:Molecular Conformation; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
chemical_list: D004798:Enzymes; D011506:Proteins; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase