Performance characteristics of an analytical procedure for determining chloramphenicol residues in muscle tissue by gas chromatography-electron capture detection.
Validation of an analytical method for determining chloramphenicol residues in muscle tissue by gas chromatography-electron capture detection (GC-ECD) was performed according to the latest European Union criteria for the analysis of veterinary drugs in food, laid down by Commission Decision 2002/657/EC. The method using the meta isomer of chloramphenicol as an internal standard proved to be very selective, specific to other related phenicols and accurate to within +3.6% at a concentration level of 8.9 microg/kg, as present in the certified reference material available. The correlation coefficient of the calibration curve was 0.9991. At all three fortification levels studied (0.3, 0.45 and 0.6 microg/kg), repeatability and intra-laboratory reproducibility were <8 and < or =9%, respectively. The decision limit (CCalpha) and detection capability (CCbeta) were 0.07 and 0.12 microg/kg, respectively. The validation results and the results of participation in an international inter-laboratory proficiency test indicate that the method presented is completely suited for regulatory control to screen and quantify chloramphenicol residues in various muscle tissues on a routine basis.
Cerkvenik-Flajs
Vesna
V
Veterinary Faculty of University of Ljubljana, Institute of Food Hygiene and Bromatology, Gerbiceva 60, SI-1000 Ljubljana, Slovenia. vesna.cerkvenik@vf.uni-lj.si
D000818:Animals; D002701:Chloramphenicol / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis / Q000379:methods; D006801:Humans; D008461:Meat Products / Q000032:analysis; D015394:Molecular Structure; D015203:Reproducibility of Results
D002701:Chloramphenicol