The NPM-RAR fusion protein associated with the t(5;17) variant of APL does not interact with PML.
The PML protein localizes to regions of the nucleus known as nuclear bodies or PODs. However, in t(15;17) Acute Promyelocytic Leukemia (APL) blasts, PML is found in a micro-punctate pattern. In order to test the hypothesis that delocalization of PML from PODs is necessary for APL, we investigated the interaction of the t(5;17) APL fusion protein NPM-RAR with PML. NPM-RAR localizes diffusely throughout the nucleoplasm. NPM-RAR does not alter the localization of PML in transfected HeLa cells, and does not associate with PML in vitro. These studies suggest that NPM-RAR does not interact with PML.
Rush
Elizabeth A
EA
Department of Medicine, University of Pittsburgh Medical Center and the University of Pittsburgh Cancer Institute, USA.
Schlesinger
Kimberly W
KW
Watkins
Simon C
SC
Redner
Robert L
RL
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D000071156:Promyelocytic Leukemia Protein; D011485:Protein Binding; D012680:Sensitivity and Specificity; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014178:Translocation, Genetic / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
C120276:NPM-RARalpha protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D015514:Oncogene Proteins, Fusion; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human