Microbial communities in the garbage composting with rice hull as an amendment revealed by culture-dependent and -independent approaches.
The diversity and succession of microbial communities during the garbage composting with rice hull as an amendment were studied by denaturing gradient gel electrophoresis (DGGE) and clone library analysis of PCR-amplified 16S ribosomal DNA (rDNA) with universal primers. Based on temperature changes, the composting process could be divided into thermophilic, cooling-down, and maturing stages. The DGGE profiles and clone library analysis revealed that the microbial community drastically changed during the composting process from the thermophilic to the maturing stages. The dominant bacterial group changed from the phylum Firmicutes in the thermophilic stage to the phylum Bacteroidetes in the maturing stage. This change in microbial communities may be significant for the composting process. The diversity of cultivated bacteria isolated from samples taken at various stages of the composting process was low. A total of 87 isolates were classified as belonging to only four different groups. These groups were also detected in the DGGE profiles and by the clone library analysis. Our study indicated that a combination of culture-dependent and -independent approaches could be very useful for monitoring both bacterial diversity and the succession of communities during the composting process. This study would be beneficial for assessing the ecological consequences of disposal of organic waste.
Takaku
Hiroaki
H
Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Higashijima 265-1, Niigata, Niigata 956-8603, Japan. htakaku@niigatayakudai.jp
Kodaira
Shoko
S
Kimoto
Ayumi
A
Nashimoto
Masayuki
M
Takagi
Masamichi
M
D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D002244:Carbon / Q000032:analysis / Q000378:metabolism; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D004586:Electrophoresis; D005735:Garbage; D015723:Gene Library; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D012275:Oryza / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D009584:Nitrogen