title: Conversion of Sphingobium chlorophenolicum ATCC 39723 to a hexachlorobenzene degrader by metabolic engineering.
abstract: The gene cassette (camA+ camB+ camC) encoding a cytochrome P-450cam variant was integrated into the nonessential gene pcpM of the pentachlorophenol degrader Sphingobium chlorophenolicum ATCC 39723 by homologous recombination. The recombinant strain could degrade hexachlorobenzene at a rate of 0.67 nmol.mg (dry weight)-1.h-1, and intermediate pentachlorophenol was also identified.
authors.affiliation: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Da-Zhong
authors.initials: DZ
authors.lastname: Yan
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Ning-Yi
authors.initials: NY
authors.lastname: Zhou
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D019475:Camphor 5-Monooxygenase / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D006581:Hexachlorobenzene / Q000378:metabolism*; D010416:Pentachlorophenol / Q000378:metabolism; D011995:Recombination, Genetic; D042301:Sphingomonadaceae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006581:Hexachlorobenzene; D010416:Pentachlorophenol; D019475:Camphor 5-Monooxygenase