[Effects of p,p'-dDE on the expression of androgen-binding protein, transferrin and inhibin B mRNA in rat sertoli cells].
OBJECTIVE
To explore effects of p, p'DDE on the expression of androgen binding protein (ABP), transferrin (Tf) and inhibin B (INH B) mRNA in testis Sertoli cells of Sprague Dawley rats.


METHODS
A method has been set up to obtain a large number of viable Sertoli cells from SD rats of 18-20 days of age. With a series of concentration p,p'-DDE (10, 30, and 50 micromol/L) co-incubating the Sertoli cells in vitro, the expression of ABP, Tf and INH B mRNA were determined by RT-PCR.


RESULTS
a) With increase of the incubated p, p'-DDE, the expression of ABP mRNA in Sertoli cells went up while that of Tf and INH B dropped in a dose-dependent manner (P < 0. 05). b) The correlation analysis among ABP, Tf and INH B showed that negative relationships were found between ABP and Tf or INH B, respectively (r = - 0. 391 3, P = 0. 032 5; r = - 0.235 2, P = 0.0158), and that positive correlation was indicated between Tf and INH B (r =0.4516, P =0.0047).


CONCLUSIONS
p,p'-DDE is a reproductive toxicant which disrupts the transcription of ABP, Tf and INH B in rat Sertoli cells so as to result in reproductive dysfunction.
Liu
Guo-Hong
GH
Department of Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Wang
Chong
C
Liu
Hong-Kai
HK
Yang
Ke-Di
KD
D000727:Androgen-Binding Protein / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D003633:Dichlorodiphenyl Dichloroethylene / Q000633:toxicity*; D007265:Inhibins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D014168:Transferrin / Q000096:biosynthesis* / Q000235:genetics
D000727:Androgen-Binding Protein; D012333:RNA, Messenger; D014168:Transferrin; C422364:inhibin B; D003633:Dichlorodiphenyl Dichloroethylene; D007265:Inhibins