Plasma glutathione peroxidase by ELISA and relationship to selenium level.
BACKGROUND
Many assays have investigated GPx activity but there are few reports of immunoreactive methods of GPx plasma determinations and their relationship with plasma selenium.


METHODS
A cross-sectional analysis of selenium and GPx was carried out on 170 adults randomly selected from the electoral role. Blood samples were collected and analysed for plasma GPx using a commercial enzyme-linked immunosorbent assay (ELISA) kit and plasma selenium using magnetic sector ICP-MS. Further investigations of GPx level were carried out using one-dimensional (1D) and two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE).


RESULTS
Plasma GPx was associated with plasma Se level (r(2)=0.24, P<0.0001). Mean (range) Se was 108.9 (67.4-268.0) microg/l. Mean (range) plasma GPx was 18.5 (3.8-43.8) microg/ml, significantly higher than the theoretical mean GPx of around 6.5-6.9 microg/ml based on ICP-MS results and the proportion of Se in intact GPx. GPx was higher in males than females and lower in current than non-smokers and ex-smokers. PAGE analysis of glycine dissociated ELISA wells revealed GPx as well as a number of unknown proteins with significant variability between high and low GPx samples.


CONCLUSIONS
Plasma GPx is correlated with Se status, however, there is considerable GPx variability between patients and ELISA methods appear to significantly overestimate true plasma GPx.
Jacobson
Glenn A
GA
School of Pharmacy, University of Tasmania, Private Bag 26, Hobart, Tasmania 7001, Australia. glenn.jacobson@utas.edu.au
Narkowicz
Christian
C
Tong
Yoke Ching
YC
Peterson
Gregory M
GM
D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D011897:Random Allocation; D012643:Selenium / Q000097:blood*
D005979:Glutathione Peroxidase; D012643:Selenium