IL-18 receptor expression on epithelial cells is upregulated by TNF alpha.
IL-18 is a multifunctional cytokine that augments both innate and acquired immunity and potentiates Th1 and Th2 reactions. We studied the expression of IL-18 receptor (IL-18R) on renal and respiratory epithelial cell lines. Both cell lines upregulated IL-18R mRNA and IL-18R membrane expression in response to TNF alpha and other proinflammatory cytokines. The function of IL-18R was confirmed by induction of IL-8 release from epithelial cells in response to recombinant IL-18. Epithelial cells may represent an important target for IL-18, mainly under inflammatory conditions associated with TNF alpha release.
Krásná
Eliska
E
Department of Immunology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
Kolesár
Libor
L
Slavcev
Antonij
A
Valhová
Sárka
S
Kronosová
Bohumíra
B
Jaresová
Marcela
M
Stríz
Ilja
I
D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics / Q000494:pharmacology; D053727:Interleukin-18 Receptor alpha Subunit; D016209:Interleukin-8 / Q000378:metabolism; D018123:Receptors, Interleukin / Q000378:metabolism*; D053724:Receptors, Interleukin-18; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects*
C508572:IL18R1 protein, human; D020382:Interleukin-18; D053727:Interleukin-18 Receptor alpha Subunit; D016209:Interleukin-8; D018123:Receptors, Interleukin; D053724:Receptors, Interleukin-18; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha