Systems pharmacogenomics in yeast.
Evaluation of: Lee W, St Onge RP, Proctor M et al.: Genome-wide requirements for resistance to functionally distinct DNA-damaging agents. PLoS Genet. 1(2), e24 (2005). Despite intense study, the mechanistic and therapeutic differences in cellular responses to DNA-damaging agents are not fully understood. In order to expand knowledge of DNA damage, the authors of this study assayed the effects of 12 anticancer agents on the complete pool of approximately 4700 homozygous deletion strains of Saccharomyces cerevisiae. The screens identified genes in well-characterized DNA, in addition to genes whose role in DNA-damage-response pathways had not previously been established.
Cahan
Patrick
P
Washington University School of Medicine, Department of Medicine, Division of Oncology, Computational Biology Program, St Louis, Missouri 63110, USA. pcahan@wustl.edu
Marsh
Sharon
S
McLeod
Howard L
HL
D000970:Antineoplastic Agents / Q000494:pharmacology*; D016000:Cluster Analysis; D004249:DNA Damage / Q000187:drug effects*; D004271:DNA, Fungal / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D019008:Drug Resistance, Neoplasm; D016681:Genome, Fungal; D030781:Organisms, Genetically Modified; D010597:Pharmacogenetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D000970:Antineoplastic Agents; D004271:DNA, Fungal