title: Transgene insertion pattern analysis using genomic walking in a transgenic mouse line.
abstract: A transgene mapping technique (Noguchi et al., Exp. Anim. 53:103-111, 2004) is described that can be used to analyze transgene integration patterns in transgenic mice. The technique was used to reveal that a transgenic mouse line (GM1-sy#116) harbored inverted and direct tandem repeats of both intact and partial pCAGGS-based transgenes in the G2 region of chromosome 1. This complicated concatenation of transgenes may have been caused by simple end-joining of DNA constructs fragmented by exposure to UV transillumination during gel-purification, and by nuclease digestion inside zygote pronuclei. The results suggest that care should be taken to avoid unwanted fragmentation during the preparation of vector constructs.
authors.affiliation: Department of Veterinary Science, National Institute of Infectious Diseases, Toyama, Tokyo, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hata
authors.forename: Naho
authors.initials: N
authors.lastname: Takekawa
authors.forename: Minako
authors.initials: M
authors.lastname: Koura
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takano
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Noguchi
authors.forename: Kozue
authors.initials: K
authors.lastname: Uchio-Yamada
authors.forename: Junichiro
authors.initials: J
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D016386:Chromosome Walking; D015698:Genomic Library; D005838:Genotype; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D011995:Recombination, Genetic; D019076:Transgenes / Q000235:genetics*