[Effects of exposure of different skin areas to low-power laser light].
The effect of helium-neon laser light of extremely low power of 0.2 mW/cm2 and wavelength 632.8 nm on the immune status of mice bearing solid tumors was studied. The evaluation of the status of tumor-bearing animals was provided by taking into account the number of immune cells, cytokine concentration (tumor necrosis factor, interleukin 2, production of nitric oxide, expression of heat shock proteins (Hsp70 and Hsp90), and activity of natural killers. The model of a solid tumor was formed by subcutaneous injection of Ehrlich carcinoma cells, and average life span of tumor-bearing mice achieved about 55 days. Different areas of the skin of tumor-bearing mice were subjected either to a single (1 min, dose 0.012 J/cm3) or repeated exposure to laser light (1 min, 48-h intervals, 30 days). Two different areas were irradiated: the thymus projection area or a hind limb with solid tumors. The results showed that chronic exposure of tumor-bearing mice in the thymus projection area, and especially, hind limb, reduced the resistance, which manifested itself in the acceleration of tumor growth and a tendency of mouse life span to decrease. On the contrary, a single exposure stimulated the antitumor immunity for several days after the exposure. The results show the expediency of further investigation of the immunomodulative effects of low-power laser light and the necessity of monitoring the immune system during laser therapy.
Glushkov
O V
OV
Novoselova
E G
EG
Cherenkov
D A
DA
Novoselova
T V
TV
Khrenov
M O
MO
Lunin
S M
SM
Chudnovskiĭ
V M
VM
Iusupov
V I
VI
Fesenko
E E
EE
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology* / Q000532:radiotherapy*; D006360:Heat-Shock Proteins / Q000378:metabolism; D006371:Helium / Q000737:chemistry; D007109:Immunity / Q000528:radiation effects*; D007376:Interleukin-2 / Q000378:metabolism; D028022:Low-Level Light Therapy; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009356:Neon / Q000737:chemistry; D009569:Nitric Oxide / Q000096:biosynthesis; D012867:Skin / Q000528:radiation effects*; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000528:radiation effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D006360:Heat-Shock Proteins; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D006371:Helium; D009569:Nitric Oxide; D009356:Neon