title: The alkaline transition of blue copper proteins, Cucumis sativus plastocyanin and Pseudomonas aeruginosa azurin.
abstract: Autoreduction of Cucumis sativus plastocyanin and Pseudomonas aeruginosa azurin took place at alkaline pHs, having been accompanied by the decrease in the intensities of the charge transfer band, Cys-S- (pi)-->Cu(II) at 597 and 626 nm, and the Cu(II)-EPR signals with small AII values of 6.5 x 10(-3) and 5.3 x 10(-3) cm(-1) for plastocyanin and azurin, respectively. Further, an extra Cu(II)-EPR signal with a large AII value of 21 x 10(-3) cm(-1) also reversibly emerged with increasing pH. Plastocyanin and azurin are in an equilibrium of the three forms at alkaline pHs.
authors.affiliation: Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa, Ishikawa 920-1192, Japan. ts0513@kenroku.kanazawa-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sakurai
mesh_terms: D001400:Azurin / Q000737:chemistry*; D018553:Cucumis sativus / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D008667:Metalloproteins / Q000737:chemistry; D010084:Oxidation-Reduction; D010970:Plastocyanin / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D008667:Metalloproteins; D001400:Azurin; D010970:Plastocyanin