[The study of ribosomal protein l15 cDNA sequences as a molecular marker in the teleostei phylogenetic analysis].
In this study, the full-length cDNAs of RPL15 (ribosomal protein L15) gene were cloned and sequenced from fifteen fishes of five orders under Teleostei. The complete ORF sequences were analyzed for phylogenetic reconstruction for the first time to evaluate the potential of RPL15 gene as a novel marker in resolving teleostean phylogenetic relationships. The resultant NJ, MP and ML trees with Anguilla japonica as the outgroup were generally resolved and largely congruent with the morphology-based tree though some incongruities were observed. The incongruities were then analyzed for some explanations. The results suggested that: (1) RPL15 gene was highly conserved during eukaryotic evolution; (2) RPL15 ORF might be a good phylogenetic marker for resolving teleostean relationships. It might be especially appropriate for the higher-level relationships (such as interordinal), and it was possibly suitable for lower-level relationships as well. The same can be true for other eukaryotes.
Xiang
Zhu
Z
Key Laboratory of the Ministry of Education for Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Zhang
Jing-Nan
JN
Song
Ping
P
Hu
Jia-Rui
JR
Zhong
Yang
Y
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics*; D005399:Fishes / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D012269:Ribosomal Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012269:Ribosomal Proteins; C043649:ribosomal protein L15