title: Versican V0 and V1 guide migratory neural crest cells.
abstract: We previously showed the selective expression of the chondroitin sulfate proteoglycans versican V0 and V1 in barrier tissues that impede the migration of neural crest cells during embryonic trunk development (Landolt, R. M., Vaughan, L., Winterhalter, K. H., and Zimmermann, D. R. (1995) Development 212, 2303-2312). To test for an active involvement of these isoforms in the guidance process, we have now established protocols to isolate intact versican V0 and V1 in quantities sufficient for functional experiments. Using stripe choice assays, we demonstrate that pure preparations of either a mixture of versican V0/V1 or V1 alone strongly inhibit the migration of multipotent Sox10/p75NTR double-positive early neural crest stem cells on fibronectin by interfering with cell-substrate adhesion. We show that this inhibition is largely core glycoprotein-dependent, as the complete removal of the glycosaminoglycan chains has only a minor effect on the inhibitory capacity. Our findings support the notion that versican variants V0 and V1 act, possibly in concert with other inhibitory molecules such as aggrecan and ephrins, in directing the migratory streams of neural crest cells to their appropriate target tissues.
authors.affiliation: Laboratory of Molecular Biology, Department of Pathology, University Hospital Zurich, CH-8091 Zurich, Switzerland.
authors.forename: Shilpee
authors.initials: S
authors.lastname: Dutt
authors.forename: Maurice
authors.initials: M
authors.lastname: Kléber
authors.forename: Mattia
authors.initials: M
authors.lastname: Matasci
authors.forename: Lukas
authors.initials: L
authors.lastname: Sommer
authors.forename: Dieter R
authors.initials: DR
authors.lastname: Zimmermann
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D011508:Chondroitin Sulfate Proteoglycans / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D006023:Glycoproteins / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D037181:Lectins, C-Type / Q000502:physiology*; D009432:Neural Crest / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism*; D053675:Versicans
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D005353:Fibronectins; D006023:Glycoproteins; D006025:Glycosaminoglycans; D037181:Lectins, C-Type; C508322:VCAN protein, human; C508324:Vcan protein, rat; D053675:Versicans