[Genome conservatism of phiKMV-like bacteriophages (T7 supergroup) active against Pseudomonas aeruginosa].
The T7-like phiKMV bacteriophage active on Pseudomonas aeruginosa was previously isolated by us and shown to have DNA resistant to many endonucleases. A loss of sensitive sites might be a consequence of a long phiKMV evolution on different hosts. To elucidate, whether this trait is shared by other similar phages, several new phiKMV-like phages were isolated from different sources and compared. All studied phiKMV-like phages formed three groups, insignificantly differing in the number and localization of endonuclease-sensitive DNA sites. This confirms that the present-day phages of this species have highly conserved genomes. Mutational "restoration" of the lost sites may be restricted by a lethal effect. The phiKMV-like phages were shown for the first time to increase the rate of in vitro accumulation of giant phiKZ-like phages of P. aeruginosa. This effect is characteristic only of phiKMV-like phages.
Burkal'tseva
M V
MV
Pleteneva
E A
EA
Shaburova
O V
OV
Kadykov
V A
VA
Krylov
V N
VN
D001434:Bacteriophage Typing; D004262:DNA Restriction Enzymes / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D016679:Genome, Viral; D017105:Pseudomonas Phages / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000821:virology*
D004279:DNA, Viral; D004262:DNA Restriction Enzymes