Preliminary X-ray diffraction analysis of crystals from the recombinantly expressed human major histocompatibility antigen HLA-B*2704 in complex with a viral peptide and with a self-peptide.
The product of the human leukocyte antigen (HLA) gene HLA-B*2704 differs from that of the prototypical subtype HLA-B*2705 by three amino acids at heavy-chain residues 77 (Ser instead of Asp), 152 (Glu instead of Val) and 211 (Gly instead of Ala). In contrast to the ubiquitous HLA-B*2705 subtype, HLA-B*2704 occurs only in orientals. Both subtypes are strongly associated with spondyloarthropathies and the peptides presented by these subtypes are suspected to play a role in disease pathogenesis. HLA-B*2704 was crystallized in complex with a viral peptide and with a self-peptide using the hanging-drop vapour-diffusion method with PEG as a precipitant. Both crystals belong to space group P2(1)2(1)2(1). Data sets were collected to 1.60 A (complex with the self-peptide pVIPR) or to 1.90 A (complex with the viral peptide pLMP2) resolution using synchrotron radiation. With HLA-B*2705 complexed with pVIPR as a search model, unambiguous molecular-replacement solutions were found for the complexes of HLA-B*2704 with both peptides.
Loll
Bernhard
B
Institut für Chemie/Kristallographie, Freie Universität Berlin, Takustrasse 6, 14195 Berlin, Germany.
Zawacka
Anna
A
Biesiadka
Jacek
J
Petter
Cordula
C
Rückert
Christine
C
Saenger
Wolfram
W
Uchanska-Ziegler
Barbara
B
Ziegler
Andreas
A
D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005978:Glutathione / Q000737:chemistry; D006680:HLA Antigens / Q000737:chemistry*; D015235:HLA-B Antigens / Q000737:chemistry*; D015796:HLA-B27 Antigen; D006801:Humans; D008285:Major Histocompatibility Complex; D016296:Mutagenesis; D010455:Peptides / Q000737:chemistry; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction
D006680:HLA Antigens; D015235:HLA-B Antigens; C510610:HLA-B*27:04 antigen; D015796:HLA-B27 Antigen; D010455:Peptides; D011994:Recombinant Proteins; D005978:Glutathione