title: Comparison of the TaqMan and LightCycler systems in pharmacogenetic testing: evaluation of CYP2C9*2/*3 polymorphisms.
abstract: BACKGROUND
  Pharmacogenetic testing for drug-metabolizing enzymes is not yet widely used in clinical practice.
  
  
  METHODS
  In an attempt to facilitate the application of this procedure, we have compared two real-time PCR-based methods, the TaqMan and the LightCycler for the pharmacogenetic evaluation of CYP2C9*2/*3 polymorphisms.
  
  
  CONCLUSIONS
  Both procedures are suitable for pharmacogenetic studies. The TaqMan procedure was less expensive in terms of cost per sample, but the TaqMan apparatus is more expensive than the LightCycler apparatus.
authors.affiliation: CEINGE-Biotecnologie Avanzate S.C.a r.l., Naples, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Toriello
authors.forename: Pasquale
authors.initials: P
authors.lastname: Meccariello
authors.forename: Cristina
authors.initials: C
authors.lastname: Mazzaccara
authors.forename: Rosanna
authors.initials: R
authors.lastname: Di Fiore
authors.forename: Carmela
authors.initials: C
authors.lastname: Esposito
authors.forename: Lucia
authors.initials: L
authors.lastname: Sacchetti
mesh_terms: D000483:Alleles; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics / Q000378:metabolism*; D065729:Cytochrome P-450 CYP2C9; D006801:Humans; D010597:Pharmacogenetics / Q000191:economics / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: C450260:CYP2C9 protein, human; D065729:Cytochrome P-450 CYP2C9; D001189:Aryl Hydrocarbon Hydroxylases; D019914:Taq Polymerase