Enantiomeric separation of chiral pesticides by high-performance liquid chromatography on an amylose tris-(S)-1-phenylethylcarbamate chiral stationary phase.
Amylose tris-(S)-1-phenylethylcarbamate chiral stationary phase (CSP) was prepared. The direct enantiomeric separation of chiral pesticides on this CSP had been studied by HPLC. The mobile phase was n-hexane-isopropanol at a flow rate of 1.0 mL/min. The effects of isopropanol content and column temperature on retention and enantioselectivity were investigated. Thirty-two samples were tested, of which ten interacted enantioselectively with the CSP. Five samples were completely resolved and another five underwent near-baseline or partial resolution. The enantiomers were identified by a circular dichroism detector. Linear van't Hoff plots were established and the thermodynamic parameters were thus calculated.
Wang
Peng
P
Department of Applied Chemistry, China Agricultural University, Beijing, China.
Liu
Donghui
D
Lei
Xiaoqiang
X
Jiang
Shuren
S
Zhou
Zhiqiang
Z
D000688:Amylose / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D007202:Indicators and Reagents; D010575:Pesticides / Q000737:chemistry / Q000302:isolation & purification* / Q000592:standards; D012015:Reference Standards; D013237:Stereoisomerism; D013696:Temperature; D013816:Thermodynamics
D007202:Indicators and Reagents; D010575:Pesticides; C106151:amylose tris(1-phenylethylcarbamate); D000688:Amylose