title: Cloning, expression, purification, crystallization and preliminary crystallographic study of the protein module (BIV2-Helix) in the fusion core of bovine immunodeficiency-like virus (BIV) gp40.
abstract: The fusion core of bovine immunodeficiency virus (BIV) gp40 is proposed to be involved in membrane fusion. However, no crystal structures are yet available. A predicted protein module BIV2-Helix of BIVgp40 has been expressed in Escherichia coli and purified by chromatography. Recombinant BIV2-Helix was crystallized using the hanging-drop vapour-diffusion technique at 291 K. The crystals were grown in MPD and belonged to the primitive rhombohedral space group R3, with unit-cell parameters a = 39.17, b = 39.17, c = 295.05 A and two molecules per asymmetric unit. X-ray diffraction data were collected to 1.76 A in the home laboratory from a single crystal.
authors.affiliation: Laboratory of Structural Biology, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lou
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Meng
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Tian
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics*; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins