Mapping sequences required for nuclear localization and the transcriptional activation function of the Arabidopsis protein AINTEGUMENTA.
The Arabidopsis thaliana floral development protein AINTEGUMENTA (ANT) is a member of a large family of DNA binding proteins (AP2/ERF family) that control plant growth and development in response to developmental or environmental signals. Transcriptional activation and/or repression activities have been demonstrated for several members of this protein family. We have used fusions between ANT and the GAL4 DNA binding domain to identify an 80 amino acid sequence important for the transcriptional activation function of ANT. This region shows similarity to transcriptional activation domains in other proteins, as it is rich in Ser/Thr, Gln/Asn, and acidic amino acids. We also demonstrate that ANT can activate gene expression in Arabidopsis plants through binding to a DNA sequence corresponding to an in vitro determined ANT binding site. Finally, we show that ANT is nuclear localized and that the sequence KKKR (amino acids 252-255) is required for nuclear localization of the protein.
Krizek
Beth A
BA
Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA. krizek@sc.edu
Sulli
Chidananda
C
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D002874:Chromosome Mapping; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008239:Lysine / Q000502:physiology; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000032:analysis; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D015533:Transcriptional Activation; D015003:Yeasts / Q000235:genetics
C099055:AINTEGUMENTA protein, Arabidopsis; D029681:Arabidopsis Proteins; D005656:Fungal Proteins; D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D008239:Lysine