Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit.
The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcepsilonRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.
Burastero
S E
SE
San Raffaele Scientific Institute, via Olgettina 58, 20132 Milan, Italy. s.burastero@hsr.it
Paolucci
C
C
Breda
D
D
Monasterolo
G
G
Rossi
R E
RE
Vangelista
L
L
D000818:Animals; D000911:Antibodies, Monoclonal; D001491:Basophils / Q000378:metabolism*; D015550:Cell Degranulation / Q000276:immunology; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005189:False Positive Reactions; D006801:Humans; D015289:Leukotrienes / Q000378:metabolism*; D051379:Mice; D011933:Reagent Kits, Diagnostic / Q000592:standards; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D015289:Leukotrienes; D011933:Reagent Kits, Diagnostic