Determination of phthalates in milk and milk products by liquid chromatography/tandem mass spectrometry.
A liquid chromatographic/tandem mass spectrometric method using pneumatically assisted electrospray ionisation (LC/ESI-MS/MS) was developed for determination of dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), di-'isononyl' phthalate (DINP) and di-'isodecyl' phthalate (DIDP) in milk and milk products including infant formulas. The phthalates were extracted by a mixture of tert-butyl methyl ether and hexane from liquid samples. DBP, BBP and DEHP were removed from fats by liquid/liquid extraction into acetonitrile while DINP and DIDP were cleaned up on deactivated silica. The phthalates were detected in positive ion mode after separation on a reversed-phase C5 analytical column. Two transition products were monitored for each compound. The detection limits related to the transition products of lowest abundance were in the range 5-9 microg/kg.
Sørensen
Lambert K
LK
Steins Laboratorium, Blomstervej 1, 8381 Tilst, Denmark. lks@steins.dk
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D043302:Cultured Milk Products / Q000737:chemistry; D004785:Environmental Pollutants / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D010795:Phthalic Acids / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D004785:Environmental Pollutants; D010795:Phthalic Acids; C032279:phthalic acid