title: Identification of prion protein gene polymorphisms in goats from Italian scrapie outbreaks.
abstract: Susceptibility to scrapie in sheep is influenced by polymorphisms of the prion protein (PrP) gene, whereas no strong association between genetics and scrapie has yet been determined in goats due to the limited number of studies on these animals. In this case-control study on 177 goats from six Italian scrapie outbreaks, the association between PrP alleles and the occurrence of scrapie was studied. Three silent mutations and 11 PrP polymorphisms were identified, of which two polymorphisms (L133Q and M137I) and one silent mutation (T202T) have not been reported previously. Twelve alleles were determined by cloning. Statistical analysis suggested a possible protective role against scrapie for the glutamine to lysine mutation at codon 222.
authors.affiliation: CEA (Centro di referenza per le Encefalopatie Animali), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Turin, Italy.
authors.forename: P L
authors.initials: PL
authors.lastname: Acutis
authors.affiliation: Central Institute for Animal Disease Control (CIDC-Lelystad), PO Box 2004, 8203 AA Lelystad, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Bossers
authors.affiliation: Central Institute for Animal Disease Control (CIDC-Lelystad), PO Box 2004, 8203 AA Lelystad, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Priem
authors.affiliation: CEA (Centro di referenza per le Encefalopatie Animali), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Turin, Italy.
authors.forename: M V
authors.initials: MV
authors.lastname: Riina
authors.affiliation: CEA (Centro di referenza per le Encefalopatie Animali), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Turin, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Peletto
authors.affiliation: CEA (Centro di referenza per le Encefalopatie Animali), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Turin, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Mazza
authors.affiliation: CEA (Centro di referenza per le Encefalopatie Animali), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Turin, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Casalone
authors.affiliation: Istituto di Ricerche Farmacologiche Mario Negri, Via Eritrea 62, 20157 Milan, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Forloni
authors.affiliation: CEA (Centro di referenza per le Encefalopatie Animali), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Turin, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Ru
authors.affiliation: CEA (Centro di referenza per le Encefalopatie Animali), Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Turin, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Caramelli
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D004196:Disease Outbreaks; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D015511:Goat Diseases / Q000453:epidemiology* / Q000235:genetics; D006041:Goats; D007558:Italy / Q000453:epidemiology; D009154:Mutation; D011110:Polymorphism, Genetic; D011328:Prions / Q000235:genetics* / Q000378:metabolism; D012608:Scrapie / Q000453:epidemiology* / Q000235:genetics
chemical_list: D011328:Prions