title: Pleomorphic adenoma arising in an accessory lacrimal gland of Wolfring.
abstract: OBJECTIVE
  To describe a patient with pleomorphic adenoma arising in an accessory lacrimal gland of Wolfring in the lower lid and to illustrate the immunohistochemical and molecular cytogenetics.
  
  
  METHODS
  Single interventional case report.
  
  
  METHODS
  A 62-year-old man presented with a 20-year history of a painless slowly growing mass at the temporal part of the right lower eyelid. Histological, immunohistochemical, and fluorescence in situ hybridization studies of the excised tumor were performed.
  
  
  RESULTS
  Histological evaluation showed many glandular elements embedded in a myxoid stroma. The tumor was situated beneath an area of a normal accessory lacrimal gland of Wolfring and in close association with normal meibomian glands. Myoepithelial tumor cells in the myxoid stroma reacted strongly with an antibody against glial fibrillary acidic protein, which did not bind to normal lacrimal gland tissue. Tumor cells with both epithelial and myoepithelial morphologies reacted positively for both pleomorphic adenoma gene-1 and high-mobility group A2 proteins. Fluorescence in situ hybridization analysis showed no evidence of clonal translocations or numerical abnormalities involving chromosome 8 or 12.
  
  
  CONCLUSIONS
  Pleomorphic adenoma of the accessory lacrimal gland is an exceedingly rare tumor of the ocular adnexa. Glial fibrillary acidic protein seems to be a tumor-associated antigen. Genetically, this case of pleomorphic adenoma arising from an accessory lacrimal gland of Wolfring is identical with those originating from salivary glands.
authors.affiliation: Eye Pathology Institute, University of Copenhagen, Copenhagen, Denmark. gaja@eyepath.ku.dk
authors.forename: Ghassan A
authors.initials: GA
authors.lastname: Alyahya
authors.forename: Göran
authors.initials: G
authors.lastname: Stenman
authors.forename: Fredrik
authors.initials: F
authors.lastname: Persson
authors.forename: Jan U
authors.initials: JU
authors.lastname: Prause
authors.forename: Karen
authors.initials: K
authors.lastname: Skjødt
authors.forename: Jon P
authors.initials: JP
authors.lastname: Saunte
authors.forename: Steffen
authors.initials: S
authors.lastname: Heegaard
mesh_terms: D008949:Adenoma, Pleomorphic / Q000737:chemistry / Q000473:pathology* / Q000601:surgery; D014408:Biomarkers, Tumor / Q000032:analysis; D005142:Eyelid Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007124:Immunoenzyme Techniques; D017404:In Situ Hybridization, Fluorescence; D007766:Lacrimal Apparatus Diseases / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged
chemical_list: D014408:Biomarkers, Tumor