title: In vitro evaluation of the antibacterial activity of Arctium lappa as a phytotherapeutic agent used in intracanal dressings.
abstract: The discovery of natural biocomponents from plants with antibacterial activity on endodontic microbiota may lead to new therapies. This study evaluated the antibacterial activity of a phytotherapeutic agent prepared from an ethyl acetate fraction (AcOEt) extracted from Arctium lappa. This agent was compared with calcium hydroxide as an intracanal dressing. Twenty-seven maxillary canines were instrumented, sterilized and inoculated with a mixed bacterial suspension of Pseudomonas aeruginosa, Escherichia coli, Lactobacillus acidophilus, Streptococcus mutans and Candida albicans. The teeth were divided into three groups and their canals filled with: group 1, calcium hydroxide and propylene glycol; group 2, a paste containing AcOEt fraction of A. lappa and propylene glycol; group 3, propylene glycol (control). At 7, 14 and 30 days, three teeth from each group were opened and a paper point was placed in the root canal for 5 min. The paper points were transferred to Petri dishes with Brain Heart Infusion (BHI). The bacterial growth was classified. Mild bacterial growth was found in group 1 at all time intervals; in group 2 there was severe growth at 7 days, but no growth at 14 and 30 days. The phytotherapeutic agent extracted from an AcOEt fraction of A. lappa inhibited the growth of all the microorganisms in this study.
authors.affiliation: University of Ribeirão Preto, Av Costábile Romano, 2201, Ribeirânia, Ribeirão Preto, SP, Brazil, 14096-380.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gentil
authors.forename: Juliana Vianna
authors.initials: JV
authors.lastname: Pereira
authors.forename: Yara T Corrêa Silva
authors.initials: YT
authors.lastname: Sousa
authors.forename: Rosimeire
authors.initials: R
authors.lastname: Pietro
authors.forename: Manoel D Sousa
authors.initials: MD
authors.lastname: Neto
authors.forename: Luiz Pascoal
authors.initials: LP
authors.lastname: Vansan
authors.forename: Suzelei
authors.initials: S
authors.lastname: de Castro França
mesh_terms: D000085:Acetates / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D031170:Arctium / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D001458:Bandages / Q000382:microbiology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D002177:Candidiasis / Q000188:drug therapy; D003786:Dental Pulp Cavity / Q000187:drug effects / Q000382:microbiology*; D006801:Humans; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018915:Root Canal Preparation / Q000379:methods; D013997:Time Factors
chemical_list: D000085:Acetates; D000900:Anti-Bacterial Agents; D010936:Plant Extracts; C007650:ethyl acetate; D002126:Calcium Hydroxide