Assessment of GaN chips for culturing cerebellar granule neurons.
In this work, the behaviors of cerebellar granule neurons prepared from 7-day-old Wistar rats on gallium nitride (GaN) were investigated. We believe that this is the first time that the GaN has been used as a substrate for neuron cultures to examine its effect on cell response in vitro. The GaN surface structure and its relationship with cells were examined by atomic force microscopy (AFM), metallography microscopy, scanning electron microscopy (SEM), lactate dehydrogenase (LDH) release and Western blot analysis. GaN is a so-called III-V compound semiconductor material with a wide bandgap and a relatively high bandgap voltage. Compared with silicon used for most neural chips, neurons seeded on GaN were able to form an extensive neuritic network and expressed very high levels of GAP-43 coincident with the neurite outgrowth. Therefore, the GaN structure may spatially mediate cellular response that can promote neuronal cell attachment, differentiation and neuritic growth. The favorable biocompatibility characteristics of GaN can be used to measure electric signals from networks of neuronal cells in culture to make it a possible candidate for use in a microelectrode array.
Young
Tai-Horng
TH
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan, ROC. thyoung@ha.mc.ntu.edu.tw
Chen
Chi-Ruei
CR
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002531:Cerebellum / Q000166:cytology; D019922:GAP-43 Protein / Q000032:analysis; D005708:Gallium / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D046228:Microarray Analysis / Q000379:methods*; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D012825:Silicon / Q000494:pharmacology
D019922:GAP-43 Protein; C473348:gallium nitride; D005708:Gallium; D007770:L-Lactate Dehydrogenase; D012825:Silicon