Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection.
In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion of LSA-3 gene was cloned, and its recombinant protein (rLSA-3) was expressed in Escherichia coli and purified by column chromatography. The purified rLSA-3 and 120 test blood/serum samples collected from inhabitants in malaria-endemic areas of Mandalay, Myanmar were used for this study. In microscopic examinations of blood samples, P. falciparum positive rate was 39.1% (47/120) in thin smear trials, and 33.3% (40/120) in thick smear trials. Although the positive rate associated with the rLSA-3 (30.8%) was lower than that of the blood stage antigens (70.8%), rLSA-3 based enzyme-linked immunosorbent assay could detect 12 seropositive cases (10.0%), in which blood stage antigens were not detected. These results indicate that the LSA-3 is a useful antigen for an early serodiagnosis of P. falciparum infection.
Lee
Hyeong-Woo
HW
Division of Tropical and Endemic Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul.
Moon
Sung-Ung
SU
Ryu
Hye-Sun
HS
Kim
Yeon-Joo
YJ
Cho
Shin-Hyeong
SH
Chung
Gyung-Tae
GT
Lin
Khin
K
Na
Byoung-Kuk
BK
Kong
Yoon
Y
Chung
Kyung-Suk
KS
Kim
Tong-Soo
TS
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D017125:Genes, Protozoan / Q000235:genetics / Q000276:immunology; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood / Q000175:diagnosis*; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000276:immunology*; D010966:Plasmodium vivax / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
D000953:Antigens, Protozoan; D017931:DNA Primers; D016054:DNA, Protozoan; C063362:QF116 antigen, Plasmodium falciparum; D011994:Recombinant Proteins; C067302:liver stage-specific antigen, Plasmodium