ASIC3-immunoreactive neurons in the rat vagal and glossopharyngeal sensory ganglia.
ASIC3-immunoreactivity (ir) was examined in the rat vagal and glossopharyngeal sensory ganglia. In the jugular, petrosal and nodose ganglia, 24.8%, 30.8% and 20.6% of sensory neurons, respectively, were immunoreactive for ASIC3. These neurons were observed throughout the ganglia. A double immunofluorescence method demonstrated that many ASIC3-immunoreactive (ir) neurons co-expressed calcitonin gene-related peptide (CGRP)- or vanilloid receptor subtype 1 (VRL-1)-ir in the jugular (CGRP, 77.8%; VRL-1, 28.0%) and petrosal ganglia (CGRP, 61.7%; VRL-1, 21.5%). In the nodose ganglion, however, such neurons were relatively rare (CGRP, 6.3%; VRL-1, 0.4%). ASIC3-ir neurons were mostly devoid of tyrosine hydroxylase in these ganglia. However, some ASIC3-ir neurons co-expressed calbindin D-28k in the petrosal (5.5%) and nodose ganglia (3.8%). These findings may suggest that ASIC3-containing neurons have a wide variety of sensory modalities in the vagal and glossopharyngeal sensory ganglia.
Fukuda
T
T
Department of Oral and Maxillofacial Rehabilitation, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8525, Japan.
Ichikawa
H
H
Terayama
R
R
Yamaai
T
T
Kuboki
T
T
Sugimoto
T
T
D062565:Acid Sensing Ion Channels; D000818:Animals; D064026:Calbindins; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D017950:Ganglia, Sensory / Q000166:cytology*; D005930:Glossopharyngeal Nerve / Q000166:cytology*; D007150:Immunohistochemistry / Q000379:methods; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism*; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D015222:Sodium Channels / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014630:Vagus Nerve / Q000166:cytology*
C568269:ASIC3 protein, rat; D062565:Acid Sensing Ion Channels; D064026:Calbindins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G; D015222:Sodium Channels; D050916:TRPV Cation Channels; C476722:Trpv2 protein, rat; D014446:Tyrosine 3-Monooxygenase; D015740:Calcitonin Gene-Related Peptide