title: Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus.
abstract: Acetylene hydratase is a tungsten-containing hydroxylase that converts acetylene to acetaldehyde in a unique reaction that requires a strong reductant. The subsequent disproportionation of acetaldehyde yields acetate and ethanol. Crystals of the tungsten/iron-sulfur protein acetylene hydratase from Pelobacter acetylenicus strain WoAcy 1 (DSM 3246) were grown by the vapour-diffusion method in an N2/H2 atmosphere using polyethylene glycol as precipitant. Growth of crystals suitable for X-ray analysis strictly depended on the presence of Ti(III) citrate or dithionite as reducing agents.
authors.affiliation: Abteilung Molekulare Strukturbiologie, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany. oeinsle@gwdg.de
authors.forename: Oliver
authors.initials: O
authors.lastname: Einsle
authors.forename: Holger
authors.initials: H
authors.lastname: Niessen
authors.forename: Dietmar J
authors.initials: DJ
authors.lastname: Abt
authors.forename: Grazyna
authors.initials: G
authors.lastname: Seiffert
authors.forename: Bernhard
authors.initials: B
authors.lastname: Schink
authors.forename: Robert
authors.initials: R
authors.lastname: Huber
authors.forename: Albrecht
authors.initials: A
authors.lastname: Messerschmidt
authors.forename: Peter M H
authors.initials: PM
authors.lastname: Kroneck
mesh_terms: D000595:Amino Acid Sequence; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D003460:Crystallization; D020564:Deltaproteobacteria / Q000201:enzymology*; D006836:Hydro-Lyases / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D012984:Software; D017356:Synchrotrons; D014414:Tungsten / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D006836:Hydro-Lyases; C096200:acetylene hydratase; D014414:Tungsten