[Combined enzymatic modification of stevioside and rebaudioside A].
Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside and rebaudiosides A with the use of starch as a donor. CGTases produced by B. stearothermophilus B-5076 B. macerans BIO-4m were the most effective biocatalysts. This method can be successfully used for direct transglycosylation of stevia extract without purification of its individual components.
Kochikian
V T
VT
Markosian
A A
AA
Abelian
L A
LA
Balaian
A M
AM
Abelian
V A
VA
D001407:Bacillus / Q000201:enzymology; D002853:Chromatography, Liquid; D045786:Diterpenes, Kaurane / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry*; D005964:Glucosyltransferases / Q000737:chemistry; D006031:Glycosylation; D013213:Starch / Q000737:chemistry; D036625:Stevia / Q000737:chemistry*; D013549:Sweetening Agents / Q000737:chemistry*
D045786:Diterpenes, Kaurane; D005960:Glucosides; D013549:Sweetening Agents; C012043:stevioside; D013213:Starch; C025474:rebaudioside A; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase