Crystallization and preliminary X-ray analysis of cytosolic alpha-mannosidase from Thermotoga maritima.
Class II alpha-mannosidase cleaves off alpha-1,2-, alpha-1,3- and alpha-1,6-mannose residues. In this paper, the crystallization and preliminary X-ray analysis of cytosolic class II alpha-mannosidase from Thermotoga maritima (TM1851), a family 38 glycoside hydrolase, is described. The crystal of recombinant TM1851 belongs to the C-centred monoclinic space group C2, with unit-cell parameters a = 244.7, b = 87.4, c = 166.6 A, beta = 124.7 degrees. X-ray diffraction data were collected to a resolution of 2.9 A.
Nakajima
Masahiro
M
Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
Fushinobu
Shinya
S
Imamura
Hiromi
H
Shoun
Hirofumi
H
Wakagi
Takayoshi
T
D003460:Crystallization; D018360:Crystallography, X-Ray; D003600:Cytosol / Q000201:enzymology; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D005810:Multigene Family; D020124:Thermotoga maritima / Q000201:enzymology*; D043323:alpha-Mannosidase / Q000737:chemistry* / Q000145:classification / Q000378:metabolism
D043323:alpha-Mannosidase; D008358:Mannose