title: Landscape of the hnRNP K protein-protein interactome.
abstract: The heterogeneous nuclear ribonucleoprotein K is an ancient RNA/DNA-binding protein that is involved in multiple processes that compose gene expression. The pleiotropic action of K protein reflects its ability to interact with different classes of factors, interactions that are regulated by extracellular signals. We used affinity purification and MS to better define the repertoire of K protein partners. We identified a large number of new K protein partners, some typically found in subcellular compartments, such as plasma membrane, where K protein has not previously been seen. Electron microscopy showed K protein in the nucleus, cytoplasm, mitochondria, and in vicinity of plasma membrane. These observations greatly expanded the view of the landscape of K protein-protein interaction and provide new opportunities to explore signal transduction and gene expression in several subcellular compartments.
authors.affiliation: Department of Gastroenterology, Medical Center for Postgraduate Education and Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Mikula
authors.forename: Artur
authors.initials: A
authors.lastname: Dzwonek
authors.forename: Jakub
authors.initials: J
authors.lastname: Karczmarski
authors.forename: Tymon
authors.initials: T
authors.lastname: Rubel
authors.forename: Michał
authors.initials: M
authors.lastname: Dadlez
authors.forename: Lucjan S
authors.initials: LS
authors.lastname: Wyrwicz
authors.forename: Karol
authors.initials: K
authors.lastname: Bomsztyk
authors.forename: Jerzy
authors.initials: J
authors.lastname: Ostrowski
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000737:chemistry*; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000473:pathology; D013058:Mass Spectrometry / Q000379:methods; D025941:Protein Interaction Mapping; D040901:Proteomics / Q000379:methods*; D051381:Rats; D015398:Signal Transduction; D013347:Subcellular Fractions
chemical_list: D034641:Heterogeneous-Nuclear Ribonucleoprotein K