Phenotypic comparison of multiple monocyte-related populations in murine peripheral blood and bone marrow.
BACKGROUND
Monocyte subsets are not well defined in murine peripheral blood (PB). Monocyte-related populations could also be located in bone marrow (BM), but studies correlating monocyte populations found in these two tissues are lacking. This study simultaneously analyzed PB and BM to phenotypically define multiple monocyte-related subsets in each location.


METHODS
Murine PB and BM cells were simultaneously stained for monocyte-related populations, using five-color flow cytometry. Relevant subsets were defined on the basis of Ly-6C, CD11b, and wheat germ agglutinin phenotype in addition to light-scatter characteristics. These populations were extensively characterized for the expression of other myeloid and dendritic cell markers, adhesion molecules, chemokine receptors, and growth factor receptors.


RESULTS
Six monocyte-related populations were defined, three each in BM and PB. No identical populations were found between the two tissues. Two BM populations and one PB population have heterogeneous expression of many markers, suggesting additional complexity among monocyte-related subsets.


CONCLUSIONS
The murine monocytic series comprises multiple subsets, differing between PB and BM. This study defines and extensively phenotypes six of these populations, providing preliminary information about possible developmental relationships and migratory capacities of these cells.
Schlueter
Annette J
AJ
Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, 52242, USA. schluetera@uihc.uiowa.edu
Glasgow
Judith K
JK
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D039481:CD11b Antigen / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000737:chemistry / Q000166:cytology*; D010641:Phenotype; D013047:Specific Pathogen-Free Organisms; D014909:Wheat Germ Agglutinins / Q000032:analysis*
D000950:Antigens, Ly; D015415:Biomarkers; D039481:CD11b Antigen; C494192:Ly-6C antigen, mouse; D014909:Wheat Germ Agglutinins