title: Recognition imaging with a DNA aptamer.
abstract: We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (approximately 90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.
authors.affiliation: Department of Physics, Biodesign Institute, Arizona State University, Tempe, AZ, USA.
authors.forename: Liyun
authors.initials: L
authors.lastname: Lin
authors.forename: Hongda
authors.initials: H
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
authors.forename: Stuart
authors.initials: S
authors.lastname: Lindsay
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006801:Humans; D007073:Immunoglobulin E / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D013917:Thrombin / Q000737:chemistry
chemical_list: D000538:Aluminum Silicates; D052157:Aptamers, Nucleotide; D007073:Immunoglobulin E; D004247:DNA; D013917:Thrombin; C011934:mica