Liquid Tissue: proteomic profiling of formalin-fixed tissues.
Identification and quantitation of candidate biomarker proteins in large numbers of individual tissues is required to validate specific proteins, or panels of proteins, for clinical use as diagnostic, prognostic, toxicological, or therapeutic markers. Mass spectrometry (MS) provides an exciting analytical methodology for this purpose. Liquid Tissue MS protein preparation allows researchers to utilize the vast, already existing, collections offormalin-fixed paraffin-embedded (FFPE) tissues for the procurement of peptides and the analysis across a variety of MS platforms.
Prieto
DaRue A
DA
SAIC-Frederick, Inc., National Cancer Institute, Frederick, MD, USA.
Hood
Brian L
BL
Darfler
Marlene M
MM
Guiel
Thomas G
TG
Lucas
David A
DA
Conrads
Thomas P
TP
Veenstra
Timothy D
TD
Krizman
David B
DB
D002853:Chromatography, Liquid; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000473:pathology; D005557:Formaldehyde / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000032:analysis*; D016612:Paraffin Embedding; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016707:Tissue Fixation
D009363:Neoplasm Proteins; D005557:Formaldehyde