title: Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.
abstract: The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, 48-421, 77 Massachusetts Ave., Cambridge, MA 02139, USA.
authors.forename: Dana E
authors.initials: DE
authors.lastname: Hunt
authors.forename: Vanja
authors.initials: V
authors.lastname: Klepac-Ceraj
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Acinas
authors.forename: Clement
authors.initials: C
authors.lastname: Gautier
authors.forename: Stefan
authors.initials: S
authors.lastname: Bertilsson
authors.forename: Martin F
authors.initials: MF
authors.lastname: Polz
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D015723:Gene Library; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D012338:RNA, Ribosomal, 23S