Comparison of the compact dry CF with the most probable number method (AOAC official method 966.24) for enumeration of coliform bacteria in raw meats. Performance-Tested Methods" 110401.
Compact Dry CF is a ready-to-use test method for the enumeration of coliform bacteria in food. The plates are presterilized and contain culture medium and a cold water-soluble gelling agent. The medium should be rehydrated with 1 mL diluted sample inoculated into the center of the self-diffusible medium, allowing the solution to diffuse by capillary action. The plate can be incubated at 35 degrees C for 20-24 h and the colonies counted without any further working steps. The Compact Dry CF medium plates were validated with 5 different raw meats. The performance tests were conducted at 35 degrees C. In all studies performed, no apparent differences were observed between the Compact Dry CF method and the AOAC Official Method 966.24 results. For the accuracy claim (n = 75), a correlation factor of r2 = 0.91 (coliform) could be assigned, as stated in the application for Performance-Tested Method. No significant variations in coliform bacterial counts were observed with different production lots or plates of diverse storage age by the quality consistency and storage robustness studies.
Kodaka
Hidemasa
H
Nissui Pharmaceutical Co. Ltd., 1075-2 Hokunanmoro, Yuki, Ibaraki 307-0036, Japan. h-kodaka@yki.nissui-pharm.jp
Teramura
Hajime
H
Nirazuka
Tadanobu
T
Mizuochi
Shingo
S
D000818:Animals; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000378:metabolism*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D008461:Meat Products / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012756:Sheep; D013552:Swine; D013696:Temperature; D013997:Time Factors