title: Microglial expression of chemokine receptor CCR5 during rat forebrain development and after perinatal hypoxia-ischemia.
abstract: The chemokine macrophage inflammatory protein 1alpha (CCL3) is expressed by immune cells in the normal and injured perinatal brain. To determine whether the chemokine receptor CCR5 is a relevant target for CCL3 in the brain, we used RT-PCR and immunocytochemistry to assess changes in CCR5 expression and localization in developing normal and injured rat forebrain. CCR5 protein was expressed predominately by resting and activated microglia until 2 weeks of age. Neonatal hypoxia-ischemia increased CCR5 mRNA expression while causing CCR5 internalization, indicating receptor activation. These data implicate CCR5 in microglial recruitment and activation during brain development and after neonatal brain injury.
authors.affiliation: Department of Neurology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
authors.forename: Rita M
authors.initials: RM
authors.lastname: Cowell
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xu
authors.forename: Jack M
authors.initials: JM
authors.lastname: Parent
authors.forename: Faye S
authors.initials: FS
authors.lastname: Silverstein
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D020925:Hypoxia-Ischemia, Brain / Q000503:physiopathology*; D007150:Immunohistochemistry; D017628:Microglia / Q000378:metabolism*; D018613:Microscopy, Confocal; D016548:Prosencephalon / Q000254:growth & development* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D019713:Receptors, CCR5 / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D019713:Receptors, CCR5