Rhesus rhadinovirus R15 encodes a functional homologue of human CD200.
A viral CD200 homologue (vCD200) encoded by open reading frame R15 of rhesus rhadinovirus (RRV), a gammaherpesvirus closely related to human herpesvirus 8 (HHV-8), is described here. RRV vCD200 shares 30% and 28% amino acid identity with human CD200 (huCD200) and HHV-8 vCD200, respectively. In vitro analysis indicated that an Fc fusion (vCD200-Fc) is expressed as a glycoprotein with a core molecular mass of 53 kDa. Utilizing monoclonal antibodies raised against vCD200-Fc, vCD200 expression was detected on the surfaces of and within supernatants from infected fibroblasts. Furthermore, in vitro assays demonstrated that vCD200-Fc treatment of monocyte-derived macrophages reduces tumor necrosis factor transcript and protein levels, implying that RRV encodes a functional vCD200.
Langlais
Carly L
CL
Vaccine and Gene Therapy Institute, Oregon Health & Science University, West Campus, 505 N.W. 185th Avenue, Beaverton, Oregon 97006, USA.
Jones
John M
JM
Estep
Ryan D
RD
Wong
Scott W
SW
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016466:CHO Cells; D006224:Cricetinae; D005347:Fibroblasts / Q000821:virology; D006801:Humans; D008253:Macaca mulatta / Q000821:virology*; D008264:Macrophages / Q000821:virology; D008969:Molecular Sequence Data; D018144:Rhadinovirus / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D015703:Antigens, CD; D014764:Viral Proteins; C042739:antigens, CD200