Liquid chromatographic determination of fosthiazate residues in environmental samples and application of the method to a fosthiazate field dissipation study.
A method was developed and validated for the determination of residues of the organophosphorus nematicide fosthiazate in soil and water by using reversed-phase liquid chromatography with UV detection. Good recoveries (>85%) of fosthiazate residues were obtained from water samples (drinking water, groundwater, and liquid chromatography water) after passage of 0.5-2 L water through solid-phase extraction (SPE) C-18 cartridges and subsequent elution with ethyl acetate. Residues in soil were extracted with methanol-water (75 + 25, v/v) on a wrist-action shaker, and the extract was cleaned up on C-18 SPE cartridges before analysis. The method was validated by analysis of a range of soils with different physicochemical characteristics; recoveries exceeded 87% at fortification levels ranging from 0.02 to 5.0 mg/kg. The precision values obtained for the method, expressed as repeatability and reproducibility, were satisfactory (<11%). Fosthiazate detection limits were 0.025 microg/L and 0.005 mg/kg for water and soil samples, respectively. The decline in the levels of fosthiazate residues in soil was measured after application of fosthiazate to protected tomato cultivation. The dissipation of fosthiazate residues followed first-order kinetics with a calculated half-life of 21 days.
Tsiropoulos
Nicholas G
NG
University of Thessaly, Crop Production and Rural Environment, Department of Agriculture, Analytical Chemistry and Pesticides Laboratory, Fytokou Str, 384 46, Nea Ionia-Volos, Greece. ntsirop@uth.gr
Likas
Dimitrios T
DT
Karpouzas
Dimitrios G
DG
D000085:Acetates / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002845:Chromatography / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D007700:Kinetics; D000432:Methanol / Q000032:analysis / Q000737:chemistry; D008956:Models, Chemical; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis; D015203:Reproducibility of Results; D012987:Soil; D012989:Soil Pollutants / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013844:Thiazoles / Q000032:analysis* / Q000737:chemistry; D053778:Thiazolidines; D013997:Time Factors; D014867:Water / Q000032:analysis / Q000737:chemistry
D000085:Acetates; D009943:Organophosphorus Compounds; D010573:Pesticide Residues; D012987:Soil; D012989:Soil Pollutants; D013844:Thiazoles; D053778:Thiazolidines; D014867:Water; C007650:ethyl acetate; C493102:fosthiazate; D000432:Methanol