title: Alteration of hTERT full-length variant expression level showed different gene expression profiles and genomic copy number changes in breast cancer.
abstract: We analyzed hTERT splicing patterns with respect to telomerase activity in breast cancer. Using a cDNA microarray in 22 cell lines, we observed the difference in expression profiling based on the different levels of full-length variant expression with 71 selected genes. Using 33 known genes that act with the telomerase complex, we performed unsupervised clustering with all cell lines, and found a clustering tendency related to the full-length variant expression level. Using array-based CGH, highly altered genomic copy number changes were found more often in MCF-7 (159 genes) than in MDA-MB-231 (109 genes) and MDA-MB-435 (49 genes), suggesting more genomic changes in MCF-7 cells. On comparing MCF-7 with MDA-MB231 and MDA-MB-435 cell lines, we identified 8 genes with different copy numbers, including dystroglycan, which is located in the p12-21.2 area of chromosome 3. In conclusion, alterations in the level of the full-length variant of hTERT showed different gene expression profiles and genomic copy number changes in breast cancer, which require further study into their cause-and-effect relationship.
authors.affiliation: Cancer Metastasis Research Center, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors.forename: Sun Y
authors.initials: SY
authors.lastname: Rha
authors.forename: Hei C
authors.initials: HC
authors.lastname: Jeung
authors.forename: Woo I
authors.initials: WI
authors.lastname: Yang
authors.forename: Jin J
authors.initials: JJ
authors.lastname: Kim
authors.forename: Tae J
authors.initials: TJ
authors.lastname: Oh
authors.forename: Sung W
authors.initials: SW
authors.lastname: An
authors.forename: Hyun C
authors.initials: HC
authors.lastname: Chung
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D016000:Cluster Analysis; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018628:Gene Dosage; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D045325:HCT116 Cells; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D007527:Isoenzymes; D019098:Telomerase