Expression of mouse liver receptor homologue 1 in embryonic stem cells is directed by a novel promoter.
Liver receptor homologue 1 (LRH-1) plays important roles in many physiological processes and embryogenesis. However, little is known about the developmental regulation of lrh-1 expression. We identified a novel transcript of mouse lrh-1 (mlrh-1v2) from embryonic stem (ES) cells. mlrh-1v2 is expressed throughout embryogenesis and in several adult tissues, while the known transcript (mlrh-1v1) appears later during embryogenesis. mlrh-1v2 expression is directed by a new promoter which displays a strong activity in ES cells. The generation of the new transcript is conserved in rats. The identification of novel mlrh-1 variant and promoter is critical for elucidating LRH-1 functions in development and adult tissues.
Gao
Da-ming
DM
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, S212, Yueyang Road 320, Shanghai 200031, China.
Wang
Lin-fang
LF
Liu
Jing
J
Kong
Yu-ying
YY
Wang
Yuan
Y
Xie
You-hua
YH
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017124:Conserved Sequence; D004622:Embryo, Mammalian / Q000166:cytology; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D014644:Genetic Variation; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D013234:Stem Cells / Q000378:metabolism*
C431936:Nr5a2 protein, mouse; D018160:Receptors, Cytoplasmic and Nuclear