title: Identification of multiple nuclear localization signals in murine Elf3, an ETS transcription factor.
abstract: We investigated nuclear localization signal (NLS) determinants within the AT-hook and ETS DNA-binding domains of murine Elf3 (mElf3), a member of the subfamily of epithelium-specific ETS transcription factors. Deletion mutants containing the AT-hook, ETS domain or both localized strictly in the nucleus, suggesting that these individual domains contain independent NLS motif(s). Within the AT-hook domain, four basic residues (244KRKR247) were critical for strong NLS activity, and two potent bipartite NLS motifs (236-252 and 249-267) were sufficient for nuclear import of mElf3, although less efficient than the full domain. In addition, one stretch of basic residues (318KKK320) within the ETS domain appears to be essential for mElf3 nuclear localization. Taken together, mElf3 contains multiple NLS motifs, which may function cooperatively to effect efficient nuclear transport.
authors.affiliation: Chabiotech Co. Ltd., Seoul 135-907, South Korea.
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Do
authors.forename: Hyuk
authors.initials: H
authors.lastname: Song
authors.forename: Heung-Mo
authors.initials: HM
authors.lastname: Yang
authors.forename: Dong-Ku
authors.initials: DK
authors.lastname: Kim
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
authors.forename: Jin-Hoi
authors.initials: JH
authors.lastname: Kim
authors.forename: Kwang-Yul
authors.initials: KY
authors.lastname: Cha
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Chung
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Kim
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000737:chemistry*; D049452:Green Fluorescent Proteins; D051379:Mice; D008853:Microscopy; D019913:Nuclear Localization Signals / Q000737:chemistry*; D050784:Proto-Oncogene Protein c-ets-1 / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry*; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; C494436:Elf3 protein, mouse; D019913:Nuclear Localization Signals; D050784:Proto-Oncogene Protein c-ets-1; D014157:Transcription Factors; D049452:Green Fluorescent Proteins