title: [Study on mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP].
abstract: The polymorphism of mtDNA D-loop of 83 individuals from 9 Chinese indigenous sheep breeds and 2 imported sheep breeds were studied with 5 endonucleases, Hinf I, Msp I, Sau3A I, Xsp I and Taq I, using PCR-RFLP. The results indicated that there existed two basic haplotypes in the region of mtDNA D-loop. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors. The averaged polymorphic degree (Pi value=0.0421%) of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very poor.
authors.affiliation: Department of Animal Science and Technology, Hebei Agriculture University, Baoding 071001, China. lixianglongcn@yahoo.com
authors.forename: Xiang-Long
authors.initials: XL
authors.lastname: Li
authors.forename: Zeng-Li
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Yuan-Fang
authors.initials: YF
authors.lastname: Gong
authors.forename: Zheng-Zhu
authors.initials: ZZ
authors.lastname: Liu
authors.forename: Qing
authors.initials: Q
authors.lastname: Jia
authors.forename: Li-Ze
authors.initials: LZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002681:China; D006239:Haplotypes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D012756:Sheep / Q000235:genetics*