The diverse involvement of heterogeneous nuclear ribonucleoprotein K in mitochondrial response to insulin.
Heterogeneous nuclear ribonucleoprotein K (hnRNP K protein) is an RNA/DNA-binding protein that acts in several compartments, including mitochondria. It integrates cellular signaling cascades with multiple processes of gene expression mechanisms. Our studies demonstrate that: (1) insulin activates the import of hnRNP K protein into mitochondria in vitro and in vivo; (2) overexpression of hnRNP K protein modulates insulin-activated mitochondrial gene expression; and (3) insulin treatment stimulates binding of hnRNP K protein to mitochondrial DNA. Based on these and our previously reported results we conclude that hnRNP K protein may be a mediator of mitochondrial response to insulin.
Dzwonek
Artur
A
Department of Gastroenterology, Medical Center for Postgraduate Education and Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Roentgena Street 5, 02-781 Warsaw, Poland.
Mikula
Michał
M
Ostrowski
Jerzy
J
D000818:Animals; D004272:DNA, Mitochondrial; D005786:Gene Expression Regulation; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000502:physiology*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
D004272:DNA, Mitochondrial; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D007328:Insulin; D012333:RNA, Messenger