title: The filarial parasite Onchocerca volvulus generates the lipid mediator prostaglandin E(2).
abstract: Prostaglandins exhibit regulatory effects on the vascular and immune system. Prostaglandin E(2) (PGE(2)) modulates T helper (Th) cell and effector cell functional reactivity, thereby promoting Th2 responses. We found significant expression of PGE(2) in male and female Onchocerca volvulus. Using immunohistology, PGE(2) was predominantly detected in the hypodermis of adult O. volvulus, the metabolically most active tissue of the filaria. In contrast, the muscles were PGE(2)-negative and the epithelia of intestine and uterus and male genital tract showed only weak staining. Oocytes were well labeled whereas embryos and sperms did not react. Less pronounced PGE(2) staining was observed in some dermal microfilariae. The expression of PGE(2) was found independent of antifilarial (ivermectin) as well as anti-endobacterial (doxycycline) treatment of O. volvulus-infected patients. PGE(2) was also demonstrated in extracts of adult worms by high-performance liquid chromatography-mass spectrometry. Release of PGE(2) from live or moribund filariae can affect the host s metabolism and immune response in favor of the filarial parasite.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Germany. nbrattig@bni-hamburg.de
authors.forename: Norbert W
authors.initials: NW
authors.lastname: Brattig
authors.forename: Arline
authors.initials: A
authors.lastname: Schwohl
authors.forename: Rainer
authors.initials: R
authors.lastname: Rickert
authors.forename: Dietrich W
authors.initials: DW
authors.lastname: Büttner
mesh_terms: D000818:Animals; D015232:Dinoprostone / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D008297:Male; D017181:Onchocerca volvulus / Q000033:anatomy & histology / Q000378:metabolism*; D009855:Onchocerciasis / Q000469:parasitology
chemical_list: D015232:Dinoprostone