[Activities of superoxide dismutase, glutathione peroxidase and catalase in erythrocytes of women smoking during pregnancy].
Oxidative stress in smoking pregnant women is assumed to be enhanced by oxidants and free radicals of tobacco smoke. Inactivation and removal of reactive oxygen species depend on reactions involving in the antioxidant defence system. The aim of our study was to recognise the efficiency of enzymatic antioxidants in erythrocytes of women smoking during pregnancy. Therefore we measured activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in red blood cells. Healthy, pregnant women (n=147) were divided into groups non-smoking (n=85) and smoking (n=62) according to questionnaire declaration and concentration of cotinine in serum and urine. During pregnancy activity o SOD increased and was higher by 20% in III in comparison to the first trimester. In smoking pregnant women activity of SOD was higher than in tobacco abstinent, however decreased and was 15% lower in the late pregnancy than in I trimester. At the beginning of pregnancy activity of GPx was higher in smoking group than in non-smoking one. In both studied groups glutathione peroxidase decreased during pregnancy and was lower in III trimester by 10% and 30% in tobacco abstinent and smoking women respectively. In non-smoking women activity o CAT increased on the course of pregnancy and was higher by 20% in III in comparison to the first trimester. In smoking group that activity was lower and decreased at the end of pregnancy to 70% of that observed in I trimester. In smoking pregnant women activity of SOD was significantly correlated with concentration of cotinine both in serum (r=0.36; p<0.05) and in urine (r=0.33; p<0.01). The similar correlation was observed between activity of GPx and level of cotinine in urine (r=0.33; p<0.01). Our results indicate that smoking during pregnancy modifies antioxidant defence system in red parameters may have a negative effect on antioxidant protection systems in neonates.
Chełchowska
Magdalena
M
Zakład Biochemii Klinicznej Instytutu Matki i Dziecka w Warszawie. lipidy@imid.med.pl
Laskowska-Klita
Teresa
T
Niemiec
Krzysztof T
KT
D000328:Adult; D002374:Catalase / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D011247:Pregnancy; D012907:Smoking / Q000097:blood* / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*
D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase