Shotgun identification of proteins from uredospores of the bean rust Uromyces appendiculatus.
We are interested in learning more about the proteome of Uromyces appendiculatus, the fungus that causes common bean rust. Knowledge of the proteins that differentiate life-cycle stages and distinguish infectious bodies such as uredospores, germlings, appressoria, and haustoria may be used to define host-pathogen interactions or serve as targets for chemical inhibition of the fungus. We have used 2-D nanoflowLC-MS/MS to identify more than 400 proteins from asexual uredospores. A majority of the proteins appear to have roles in protein folding or protein catabolism. We present a model by which an abundance of heat shock proteins and translation elongation factors may enhance a spore's ability to survive environmental stresses and rapidly initiate protein production upon germination.
Cooper
Bret
B
Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. cooperb@ba.ars.usda.gov
Garrett
Wesley M
WM
Campbell
Kimberly B
KB
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000378:metabolism*; D002853:Chromatography, Liquid; D007887:Fabaceae / Q000382:microbiology; D005656:Fungal Proteins / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D017510:Protein Folding; D040901:Proteomics / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D013172:Spores, Fungal / Q000378:metabolism*
D005656:Fungal Proteins; D010455:Peptides