title: [Immunological efficiency of DNA vaccine targeting dendritic cells against tumor].
abstract: OBJECTIVE
  To construct the DNA vaccine containing ovalbumin (OVA) and Fc fusion gene targeting dentritic cells (DCs) and evaluate its anti-tumor efficiency in an E.G7-OVA-bearing tumor model.
  
  
  METHODS
  Constructed OVA-Fc-pcDNA3.1 plasmids were transfected into CHO cells with lipofectamine and the in vitro expression of OVA-Fc was determined by flow cytometry and ELISA. (51)Cr-relaese assay was used to determine the anti-tumor activity of cytotoxic T lymphocytes (CTLs) from splenocytes of immunized mice. According to tumor volume and survival time of the mice, the therapeutic effect of this vaccine was evaluated in E.G7-OVA-bearing tumor model.
  
  
  RESULTS
  DNA sequencing and restriction endonuclease digestion analysis indicated that the recombinant expression vector OVA-Fc-pcDNA3.1 had been constructed successfully. OVA-Fc expression could be detected in CHO cells transfected with OVA-Fc-pcDNA3.1 by ELISA and flow cytometry. The DNA vaccine containing OVA-Fc fusion gene inhibited tumor's growth and prolonged the time of tumor-bearing mice due to elicit the CTL-mediated anti-tumor immunity.
  
  
  CONCLUSIONS
  OVA-Fc-pcDNA3.1 DNA vaccine targeting dendritic cells can elicit the CTL-mediated anti-tumor immunity, which lays the foundation for further clinical experiments.
authors.affiliation: Cancer Center of Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. oncology_bozhu@yahoo.com.cn
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Cheng
authors.forename: Yu-zhong
authors.initials: YZ
authors.lastname: Duan
authors.forename: Bo
authors.initials: B
authors.lastname: Guo
authors.forename: Yu-zhang
authors.initials: YZ
authors.lastname: Wu
authors.forename: Zheng-tang
authors.initials: ZT
authors.lastname: Chen
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D005822:Genetic Vectors / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000235:genetics / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D019444:Vaccines, DNA / Q000008:administration & dosage*; D023041:Xenograft Model Antitumor Assays
chemical_list: D019444:Vaccines, DNA; D010047:Ovalbumin