title: Capillary flow porometry to assess the seal provided by root-end filling materials in a standardized and reproducible way.
abstract: This in vitro study evaluated the root-end sealing ability of gutta-percha + AH26 (GP), Ketac-Fil, Fuji IX (FIX), tooth-colored MTA (MTA), IRM, Ketac-Fil + conditioner (Ketac-FilC), and Fuji IX + conditioner (FIXC). A total of 140 standardized bovine root sections were divided into seven groups, filled with the mentioned root-end filling materials, and, at 48 h, submitted to capillary flow porometry to assess minimum, mean flow and maximum pore diameters. Results were statistically analyzed using nonparametric Kruskal-Wallis and Dunn tests. Level of significance was set at 0.05. Using the Kruskal-Wallis tests we found that there was no significant difference between the minimum pore diameters of the different materials, but significant differences between the mean flow (p < 0.001) and maximum (p < 0.001) pore diameters could be demonstrated. For the mean flow pore diameters, there was a significant difference between FIX and all other materials, between Ketac-Fil and IRM and between Ketac-FilC and IRM. Concerning maximum pore diameters, there was a significant difference between FIX and all other materials, between Ketac-Fil and MTA, GP and IRM, FIXC and IRM, and Ketac-FilC and IRM. The data showed that each sample had leaked. Glass ionomer cements leaked more than other materials, although dentin conditioning diminished the maximum through pore diameters. This maximum pore diameter, which corresponds to the largest leak in the sample, together with the size of bacteria and their metabolites, will be indicative of the eventual leakage along the root-end filling materials.
authors.affiliation: Department of Operative Dentistry and Endodontology, Dental School, Ghent University, Ghent University Hospital, Gent, Belgium. mieke.debruyne@ugent.be
authors.forename: Mieke A A
authors.initials: MA
authors.lastname: De Bruyne
authors.forename: Roger J E
authors.initials: RJ
authors.lastname: De Bruyne
authors.forename: Roeland J G
authors.initials: RJ
authors.lastname: De Moor
mesh_terms: D000134:Acid Etching, Dental; D000180:Acrylic Resins; D017607:Aluminum Compounds; D000818:Animals; D001729:Bismuth; D017610:Calcium Compounds; D002417:Cattle; D003763:Dental Leakage / Q000175:diagnosis*; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; D004853:Epoxy Resins; D005899:Glass Ionomer Cements; D006171:Gutta-Percha; D008298:Maleates; D008768:Methylmethacrylates; D008833:Microcirculation; D010087:Oxides; D016062:Porosity; D015203:Reproducibility of Results; D012184:Retrograde Obturation / Q000379:methods*; D012387:Root Canal Filling Materials; D017640:Silicates; D012834:Silver; D014025:Titanium; D015035:Zinc Oxide-Eugenol Cement
chemical_list: D000180:Acrylic Resins; D017607:Aluminum Compounds; D017610:Calcium Compounds; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; D004853:Epoxy Resins; D005899:Glass Ionomer Cements; C510940:Ketac Dentin Conditioner; D008298:Maleates; D008768:Methylmethacrylates; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C110713:fuji IX; C086631:mineral trioxide aggregate; D012834:Silver; C006903:carbopol 940; C028044:epoxy resin AH-26; C031667:IRM cement; C040964:Ketac-Fil; D006171:Gutta-Percha; D014025:Titanium; D001729:Bismuth