The use of mitochondrial cytochrome oxidase I gene (COI) to differentiate two UK blowfly species -- Calliphora vicina and Calliphora vomitoria.
Traditionally identification of forensically important insects has been carried out based upon morphological differences between species. However insect evidence found at a crime scene may on occasion be difficult to distinguish by morphological techniques and under these circumstances another method of accurate identification is required. This work utilises a cytochrome oxidase I partial mitochondrial gene region (COI) to distinguish the two of the main UK blowfly species -- Calliphora vicina (Robineau Desvoidy) and Calliphora vomitoria (Linnaeus) (Diptera:Calliphoridae). Seventeen interspecific differences in COI sequence were located. Use of the restriction enzyme SfcI on this gene region provides a simple method for distinguishing between C. vicina and C. vomitoria.
Ames
Carole
C
King's College London, Department of Forensic Science and Drug Monitoring, London, UK.
Turner
Bryan
B
Daniel
Barbara
B
D000818:Animals; D004262:DNA Restriction Enzymes / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D004175:Diptera / Q000145:classification* / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D004772:Entomology; D018732:Forensic Anthropology; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV; D004262:DNA Restriction Enzymes; C041749:endodeoxyribonuclease SfiI; D015252:Deoxyribonucleases, Type II Site-Specific