title: Green polymers from Geobacillus thermodenitrificans DSM465--candidates for molecularly imprinted materials.
abstract: Novel molecular recognition materials were prepared from water soluble proteins from thermophile G. thermodenitrificans DSM465 by an alternative molecular imprinting method. Water soluble proteins from G. thermodenitrificans DSM465 were converted into the molecularly imprinted materials by adopting 9-EA as a print molecule. The molecularly imprinted protein membranes recognized As in preference to Gs. The adsorption isotherms led to the conclusion that molecular recognition sites toward As were constructed by the presence of 9-EA during the membrane preparation process. The affinity constant between As and the molecular recognition site thus constructed was determined to be 1.75 x 10(5) mol(-1) dm(3). The results obtained in the present study suggest that water soluble proteins from G. thermodenitrificans DSM465 is one of environmentally-friendly 'green' polymers to be converted into molecular recognition materials by applying an alternative molecular imprinting method.
authors.affiliation: Laboratory for Applied Polymer Chemistry, Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kawamura
authors.forename: Akinori
authors.initials: A
authors.lastname: Ejima
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sakurai
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hayashi
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000327:Adsorption; D001406:Bacillaceae / Q000737:chemistry* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D008567:Membranes, Artificial; D008968:Molecular Conformation; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012995:Solubility; D013718:Tensile Strength; D014867:Water / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; D011506:Proteins; D014867:Water