[Isolation, identification and functional characterization of human CD4+ CD25+ Treg cells from human peripheral blood].
OBJECTIVE
To isolate CD4(+) CD25(+) Treg cells from human peripheral blood, and study their functional characteristics.


METHODS
The expressions of Foxp3 in human CD4(+) CD25(+) Treg cells were test by RT-PCR. The regulatory properties of CD4(+) CD25(+) Treg cells were assessed by co-culturing with CD4(+) CD25(-) T cells and CD8(+) T cells, or adding exogenous IL-2. The detection of intracellular cytokine production of IL-4, IL-10 and IFN-gamma was done by flow cytometry.


RESULTS
CD4(+) CD25(+) Treg cells highly expressed Foxp3 and mainly synthesized IL-10 that suppressed the proliferation of CD4(+) CD25(-) T and CD8(+) T cells. Its suppressive function was reversed by high concentration of IL-2 and (or) IL-4.


CONCLUSIONS
CD4(+) CD25(+) Treg cells exhibited a subpopulation of immunoregulatory T cells with suppressive function, which could be reversed by high concentration of IL-2.
Yang
Jiang-hua
JH
Department of Infectious Diseases, the First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China. yjhpath@163.com
Zhang
Yong-xiang
YX
Wang
Fang
F
Yu
Rong-bin
RB
Su
Chuan
C
Sun
Nan-xiong
NX
D001773:Blood Cells / Q000145:classification* / Q000502:physiology; D015704:CD4 Antigens / Q000302:isolation & purification* / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000302:isolation & purification* / Q000502:physiology; D050378:T-Lymphocytes, Regulatory / Q000145:classification* / Q000502:physiology
D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit