title: Extraction of berberine from rhizome of Coptis chinensis Franch using supercritical fluid extraction.
abstract: Supercritical fluid was used to extract berberine from rhizome of Coptis chinensis Franch. The recovery of berberine was compared with various modifiers, i.e. methanol and 95% ethanol with and without surfactant Tween 80, and 1,2-propanediol. The results show that the yield obtained after 3 h extraction with 1,2-propanediol-modified supercritical carbon dioxide was the highest (from 6.91%, w/w at 200 bar to 7.53%, w/w at 500 bar), while that obtained with 95% ethanol modified-supercritical carbon dioxide was the lowest (from 0.15%, w/w at 300 bar to 0.19%, w/w at 600 bar). The recovery of berberine was not improved by adding 5% Tween 80 in supercritical fluid.
authors.affiliation: Department of Bioengineering and Pharmaceutics, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China. liu_ben0@hotmail.com
authors.forename: Ben
authors.initials: B
authors.lastname: Liu
authors.forename: Wenjing
authors.initials: W
authors.lastname: Li
authors.forename: Yiling
authors.initials: Y
authors.lastname: Chang
authors.forename: Wenhong
authors.initials: W
authors.lastname: Dong
authors.forename: Li
authors.initials: L
authors.lastname: Ni
mesh_terms: D001599:Berberine / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D039323:Coptis / Q000737:chemistry*; D012015:Reference Standards; D012997:Solvents / Q000737:chemistry*
chemical_list: D012997:Solvents; D001599:Berberine