title: Ligand profiling and identification technology for searching bioactive ligands.
abstract: We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chromatography, and thus are inevitably limited by the low abundance of the peptide of interest and by a low yield due to the many column steps. Using this new technology, insulin was successfully identified and diarginylinsulin, a minor intermediate form of insulin, was unexpectedly also identified simultaneously from 100 mg of porcine pancreatic tissue. This integrative technology could be used to search for various low-abundance peptides (or bioactive molecules) rapidly and simultaneously, by applying this to the later stages of traditional sequential purification.
authors.affiliation: Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Moon-Chang
authors.initials: MC
authors.lastname: Baek
authors.forename: Sun-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Kyungmoo
authors.initials: K
authors.lastname: Yea
authors.forename: Youndong
authors.initials: Y
authors.lastname: Kim
authors.forename: Byung-Dae
authors.initials: BD
authors.lastname: Lee
authors.forename: Jaeyoon
authors.initials: J
authors.lastname: Kim
authors.forename: Hae-Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Mee-Hee
authors.initials: MH
authors.lastname: Kang
authors.forename: Sun-Kyu
authors.initials: SK
authors.lastname: Choi
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Kim
authors.forename: Taehoon G
authors.initials: TG
authors.lastname: Lee
authors.forename: Pann-Ghill
authors.initials: PG
authors.lastname: Suh
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Ryu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods*; D003720:Densitometry; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007328:Insulin / Q000031:analogs & derivatives / Q000032:analysis*; D008024:Ligands; D013058:Mass Spectrometry / Q000379:methods*; D036103:Nanotechnology; D010179:Pancreas / Q000737:chemistry; D010447:Peptide Hydrolases / Q000494:pharmacology; D010455:Peptides / Q000032:analysis*; D051381:Rats; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine
chemical_list: D007328:Insulin; D008024:Ligands; D010455:Peptides; C063853:insulin, Arg(B31,B32)-; D011972:Receptor, Insulin; D010447:Peptide Hydrolases