title: Sulfolobus solfataricus translation initiation factor 1 stimulates translation initiation complex formation.
abstract: The eukaryotic translation initiation factor 1 binds to the ribosome during translation initiation. It is instrumental for initiator-tRNA and mRNA binding, and has a function in selection of the authentic start codon. Here, we show that the archaeal homolog aIF1 has analogous functions. The aIF1 protein of the archaeon Sulfolobus solfataricus is bound to the small ribosomal subunit during translation initiation and accelerates binding of initiator-tRNA and mRNA to the ribosome. Accordingly, aIF1 stimulated translation of an mRNA in a S. solfataricus in vitro translation system. Moreover, this study suggested that the C terminus of the factor is of relevance for its function.
authors.affiliation: Max F. Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter, Dr. Bohrgasse 9/4, 1030 Vienna, Austria.
authors.forename: David
authors.initials: D
authors.lastname: Hasenöhrl
authors.forename: Dario
authors.initials: D
authors.lastname: Benelli
authors.forename: Alessandra
authors.initials: A
authors.lastname: Barbazza
authors.forename: Paola
authors.initials: P
authors.lastname: Londei
authors.forename: Udo
authors.initials: U
authors.lastname: Bläsi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D015977:Eukaryotic Initiation Factor-1 / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D005798:Genes, Bacterial; D006160:Guanosine Triphosphate / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000502:physiology*; D034622:RNA Interference; D019642:RNA, Archaeal / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D016944:Sulfolobus / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D015977:Eukaryotic Initiation Factor-1; D019642:RNA, Archaeal; D006160:Guanosine Triphosphate