title: The secretory granule protein syncollin localizes to HL-60 cells and neutrophils.
abstract: The secretory granule protein syncollin was first identified in the exocrine pancreas where a population of the protein is associated with the luminal surface of the zymogen granule membrane. In this study we provide first morphological and biochemical evidence that, in addition to its pancreatic localization, syncollin is also present in neutrophilic granulocytes of rat and human origin. By immunohistological studies, syncollin was detected in neutrophilic granulocytes of the spleen. Furthermore, syncollin is expressed by the promyelocytic HL-60 cells, where it is stored in azurophilic granules and in a vesicular compartment. These findings were confirmed by fractionation experiments and immunoelectron microscopy. Treatment with a phorbol ester triggered the release of syncollin indicating that in HL-60 cells it is a secretory protein that can be mobilized upon stimulation. A putative role for syncollin in host defense is discussed.
authors.affiliation: Department of Cell Biology and Cell Pathology, University of Marburg, Robert-Koch Str. 6, D-35037 Marburg, Germany.
authors.forename: Jan-Philipp
authors.initials: JP
authors.lastname: Bach
authors.forename: Heike
authors.initials: H
authors.lastname: Borta
authors.forename: Waltraud
authors.initials: W
authors.lastname: Ackermann
authors.forename: Floriane
authors.initials: F
authors.lastname: Faust
authors.forename: Oliver
authors.initials: O
authors.lastname: Borchers
authors.forename: Michael
authors.initials: M
authors.lastname: Schrader
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D018922:HL-60 Cells; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D016253:Microscopy, Immunoelectron; D009504:Neutrophils / Q000378:metabolism* / Q000648:ultrastructure; D009928:Organ Specificity; D009195:Peroxidase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D022142:Secretory Vesicles / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; C107454:Sycn protein, rat; D009195:Peroxidase