title: [The application of microsatellite markers for parentage determination in selective breeding of Pacific white shrimp (Litopenaeus vannamei)].
abstract: Used simulations and controlled mating to examine the potential of microsatellite markers in assigning parentage to Pacific white shrimp progeny. Cervus simulations demonstrated that the theoretical expectations for parentage exclusion of 10 microsatellite loci and six most polymorphic of the 10 loci were both 0.99, and the assignment success rate of the 6 most polymorphic microsatellite loci set was nearly to 0.97 with 95% confidence. Based on this information, offspring from 10 crosses where parents were known were genotyped by the 6 microsatellite loci and used for parentage analysis. The result showed that assignment success of the progeny to their 'true' mother and father was 88% and 78% respectively, which were lower than predicted by the Cervus simulations. This could be explained by the existence of null or mutant alleles and by Taq DNA Polymerase slippage in the microsatellite loci.
authors.affiliation: Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. hongxiawang007@ms.qdio.ac.cn
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Wang
authors.forename: Chang-Gong
authors.initials: CG
authors.lastname: Wu
authors.forename: Liu-Suo
authors.initials: LS
authors.lastname: Zhang
authors.forename: Jian-Hai
authors.initials: JH
authors.lastname: Xiang
mesh_terms: D000818:Animals; D018895:Microsatellite Repeats / Q000235:genetics*; D010375:Pedigree; D033561:Penaeidae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction