title: Purification, crystallization and preliminary X-ray analysis of a hexameric beta-glucosidase from wheat.
abstract: The wheat beta-glucosidase TaGlu1b, which is only active in a hexameric form, was tagged with 6xHis at the N-terminus, overexpressed in Escherichia coli and purified in two steps. The protein complexed with a substrate aglycone was crystallized at 293 K from a solution containing 10 mM HEPES pH 7.2, 1 M LiSO4 and 150 mM NaCl using the hanging-drop vapour-diffusion method. Diffraction data were collected to 1.7 A at the Photon Factory. The crystal belongs to space group P4(1)32, with unit-cell parameters a = b = c = 194.65 A, alpha = beta = gamma = 90 degrees. The asymmetric unit was confirmed by molecular-replacement solution to contain one monomer, giving a solvent content of 72.1%.
authors.affiliation: Department of Applied Biology and Chemisry, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Sue
authors.forename: Kana
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kouyama
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Kanju
authors.initials: K
authors.lastname: Ohsawa
authors.forename: Toru
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Hajime
authors.initials: H
authors.lastname: Iwamura
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yajima
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D020836:Protein Structure, Quaternary; D014908:Triticum / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry*
chemical_list: D001617:beta-Glucosidase