Normal specification of the extraembryonic lineage after somatic nuclear transfer.
To examine the establishment and maintenance of trophectoderm (TE) lineage in somatic cloned blastocysts, the expression of Cdx2, a key molecule for specification of TE fate, was immunohistochemically examined simultaneously with Oct4 expression. Cloned mouse embryos were made by nuclear transfer using cumulus cells, tail-tip fibroblasts, and embryonic stem cells. After 96 h of culture, the rates of Oct4-expressing blastocysts were as low as 50% and 60% for cumulus and fibroblast clones, respectively. However, regardless of Oct4 expression, the majority of those cloned blastocysts (> 90%) normally expressed Cdx2. Thus, even though somatic cloned embryos have reduced potential to produce the inner cell mass lineage, the TE lineage can be established and maintained.
Kishigami
Satoshi
S
Laboratory for Genomic Reprogramming, Center for Developmental Biology RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Kobe 650-0047, Japan. kiskigami@cdb.riken.jp
Hikichi
Takafusa
T
Van Thuan
Nguyen
N
Ohta
Hiroshi
H
Wakayama
Sayaka
S
Bui
Hong-Thuy
HT
Mizutani
Eiji
E
Wakayama
Teruhiko
T
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D000071616:CDX2 Transcription Factor; D018929:Cell Culture Techniques; D019070:Cell Lineage; D002467:Cell Nucleus; D002999:Clone Cells / Q000166:cytology; D004622:Embryo, Mammalian / Q000166:cytology*; D018398:Homeodomain Proteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D050814:Octamer Transcription Factor-3 / Q000032:analysis; D013234:Stem Cells / Q000166:cytology; D014157:Transcription Factors / Q000032:analysis
D000071616:CDX2 Transcription Factor; C487514:Cdx2 protein, mouse; D018398:Homeodomain Proteins; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D014157:Transcription Factors