title: Naltrexone influences protein kinase Ce and integrin alpha7 activity in SH-SY5Y neuroblastoma cells.
abstract: Alcohol influences the neuroadaptation of brain cells where receptors and enzymes like protein kinase C (PKC) exist. Naltrexone acts on opioid receptors. However, other mechanisms of action remain unknown. We prepared SH-SY5Y neuroblastoma cells, and fed them with 150 mM ethanol for 72 h followed by treatment with naltrexone for 24 h. We performed microarray analysis and reverse transcriptase-polymerase chain reaction. Our results showed that PKCepsilon increased 1.90 times and showed an overall decreasing pattern as time increased. Phosphorylated ERK also increased 2.0 times according to the change of PKCepsilon. Integrin alpha7 increased 2.32 times and showed an increasing pattern as time increased. In conclusion, naltrexone influences PKCepsilon neuronal signaling system and endothelial adhesion molecule integrin alpha7 in addition to the well-known opioid system.
authors.affiliation: Department of Psychiatry, Kwandong University, Gangneung 210-701, Korea.
authors.forename: Dong Yul
authors.initials: DY
authors.lastname: Oh
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Jung
authors.forename: Byung Hwan
authors.initials: BH
authors.lastname: Yang
authors.forename: Jun Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Ihn Geun
authors.initials: IG
authors.lastname: Choi
authors.forename: Young Gyu
authors.initials: YG
authors.lastname: Chai
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D039001:Integrin alpha Chains / Q000378:metabolism*; D009271:Naltrexone / Q000494:pharmacology*; D009447:Neuroblastoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D051744:Protein Kinase C-epsilon / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D015703:Antigens, CD; D018076:DNA, Complementary; D039001:Integrin alpha Chains; C085725:integrin alpha7; D009271:Naltrexone; D051744:Protein Kinase C-epsilon