title: Hyperbaric oxygen induces vascular endothelial growth factor and reduces liver injury in regenerating rat liver after partial hepatectomy.
abstract: OBJECTIVE
  The aim of this study was to investigate the effect and the mechanism of hyperbaric oxygen treatment on regenerating rat liver after partial hepatectomy (PH).
  
  
  METHODS
  Wistar rats underwent a 70% PH, followed by treatment with hyperbaric oxygen starting 8 h after PH. The regenerated liver weight and serum parameters were compared. Proliferation of both hepatocytes and sinusoidal endothelial cell (SEC) was also monitored by evaluating the proliferating cell nuclear antigen (PCNA) labeling index. Furthermore, the hepatic adenosine triphosphate levels and vascular endothelial growth factor (VEGF) protein expression were analyzed at different times.
  
  
  RESULTS
  Hyperbaric oxygen treatment significantly reduced the serum alanine aminotransferase levels at 24 h, total bilirubin and total bile acid levels at 48 and 72 h, respectively. No significant differences in the hepatic adenosine triphosphate levels, the restitution of liver weight, or PCNA positive hepatocytes were observed between the two groups. The PCNA positive SEC, in contrast, was significantly increased in the hyperbaric oxygen group at 48h, furthermore, the hyperbaric oxygen treatment significantly increased the expression of VEGF protein in the regenerating liver at 24 and 48 h.
  
  
  CONCLUSIONS
  Hyperbaric oxygen treatment can be considered as a therapeutic modality after massive PH.
authors.affiliation: The Department of Surgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. hiditi@surg2@med.kyushu-u.ac.jp
authors.forename: Hideki
authors.initials: H
authors.lastname: Ijichi
authors.forename: Akinobu
authors.initials: A
authors.lastname: Taketomi
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Yoshizumi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Uchiyama
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yonemura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Soejima
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Shimada
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Maehara
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D042783:Endothelial Cells / Q000166:cytology; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology; D006931:Hyperbaric Oxygenation; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008115:Liver Regeneration / Q000187:drug effects / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D014947:Wounds and Injuries / Q000517:prevention & control / Q000628:therapy
chemical_list: D042461:Vascular Endothelial Growth Factor A; D000255:Adenosine Triphosphate