The influence of cigarette smoke inhalation and its cessation on the tooth-supporting alveolar bone: a histometric study in rats.
OBJECTIVE
It has been previously shown that smoking may enhance periodontal breakdown and impair bone healing around titanium implants. However, there is a lack of information concerning the effect of smoking on the tooth-supporting alveolar bone. Thus, the aim of this study was to histometrically evaluate the influence of cigarette smoke inhalation and its cessation on tooth-supporting alveolar bone.


METHODS
Sixty male Wistar rats were randomly assigned to one of the following groups: group 1 - control (n = 15), group 2 - 2 months of cigarette smoke inhalation (n = 13), group 3 - 3 months of cigarette smoke inhalation and 2 months without exposure to cigarette smoke inhalation (n = 16) and group 4 - 5 months of cigarette smoke inhalation (n = 16). Five months after the beginning of cigarette smoke inhalation regime (2 months for group 2), the animals were killed and the mandible was removed and prepared for histological sections. The proportion of mineralized tissue in the furcation area (i.e. a 1000 microm zone under the furcation and between the roots) was obtained.


RESULTS
Data analysis demonstrated that the animals continuously exposed to cigarette smoke inhalation presented a decreased proportion of mineralized tissue (groups 2 and 4), when compared to control and cessation groups (groups 1 and 3) (p < 0.05). Similar levels of proportion of mineralized tissue were observed in groups 1 and 3, showing a beneficial effect of cigarette smoke inhalation cessation on proportion of mineralized tissue.


CONCLUSIONS
Within the limits of the present study, it can be concluded that cigarette smoke inhalation may affect the tooth-supporting bone as early as 2 months after the initial exposure, and that smoke exposure cessation may revert its negative impact on the alveolar bone.
César-Neto
João Batista
JB
Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, University of Campinas, São Paulo, Brazil.
Benatti
Bruno Braga
BB
Sallum
Enilson Antônio
EA
Casati
Márcio Zaffalon
MZ
Nociti
Francisco Humberto
FH
D016301:Alveolar Bone Loss / Q000209:etiology*; D000704:Analysis of Variance; D000818:Animals; D015519:Bone Density; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012907:Smoking / Q000009:adverse effects*; D016540:Smoking Cessation; D013997:Time Factors