title: Crystallization and preliminary X-ray analysis of cytosolic alpha-mannosidase from Thermotoga maritima.
abstract: Class II alpha-mannosidase cleaves off alpha-1,2-, alpha-1,3- and alpha-1,6-mannose residues. In this paper, the crystallization and preliminary X-ray analysis of cytosolic class II alpha-mannosidase from Thermotoga maritima (TM1851), a family 38 glycoside hydrolase, is described. The crystal of recombinant TM1851 belongs to the C-centred monoclinic space group C2, with unit-cell parameters a = 244.7, b = 87.4, c = 166.6 A, beta = 124.7 degrees. X-ray diffraction data were collected to a resolution of 2.9 A.
authors.affiliation: Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakajima
authors.forename: Shinya
authors.initials: S
authors.lastname: Fushinobu
authors.forename: Hiromi
authors.initials: H
authors.lastname: Imamura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shoun
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Wakagi
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D003600:Cytosol / Q000201:enzymology; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D005810:Multigene Family; D020124:Thermotoga maritima / Q000201:enzymology*; D043323:alpha-Mannosidase / Q000737:chemistry* / Q000145:classification / Q000378:metabolism
chemical_list: D043323:alpha-Mannosidase; D008358:Mannose