YtkD and MutT protect vegetative cells but not spores of Bacillus subtilis from oxidative stress.
ytkD and mutT of Bacillus subtilis encode potential 8-oxo-dGTPases that can prevent the mutagenic effects of 8-oxo-dGTP. Loss of YtkD but not of MutT increased the spontaneous mutation frequency of growing cells. However, cells lacking both YtkD and MutT had a higher spontaneous mutation frequency than cells lacking YtkD. Loss of either YtkD or MutT sensitized growing cells to hydrogen peroxide (H2O2) and t-butylhydroperoxide (t-BHP), and the lack of both proteins sensitized growing cells to these agents even more. In contrast, B. subtilis spores lacking YtkD and MutT were not sensitized to H2O2, t-BHP, or heat. These results suggest (i) that YtkD and MutT play an antimutator role and protect growing cells of B. subtilis against oxidizing agents, and (ii) that neither YtkD nor MutT protects spores against potential DNA damage induced by oxidative stress or heat.
Castellanos-Juárez
Francisco X
FX
Institute of Investigation in Experimental Biology, Faculty of Chemistry, University of Guanajuato, Guanajuato, 36050 Gto., Mexico.
Alvarez-Alvarez
Carlos
C
Yasbin
Ronald E
RE
Setlow
Barbara
B
Setlow
Peter
P
Pedraza-Reyes
Mario
M
D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D004260:DNA Repair / Q000502:physiology; D045643:DNA Repair Enzymes / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000633:toxicity; D009154:Mutation; D018384:Oxidative Stress; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000502:physiology*; D013171:Spores, Bacterial / Q000187:drug effects; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D001426:Bacterial Proteins; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D010744:Phosphoric Monoester Hydrolases; C078207:8-oxodGTPase; D045643:DNA Repair Enzymes