title: Normal specification of the extraembryonic lineage after somatic nuclear transfer.
abstract: To examine the establishment and maintenance of trophectoderm (TE) lineage in somatic cloned blastocysts, the expression of Cdx2, a key molecule for specification of TE fate, was immunohistochemically examined simultaneously with Oct4 expression. Cloned mouse embryos were made by nuclear transfer using cumulus cells, tail-tip fibroblasts, and embryonic stem cells. After 96 h of culture, the rates of Oct4-expressing blastocysts were as low as 50% and 60% for cumulus and fibroblast clones, respectively. However, regardless of Oct4 expression, the majority of those cloned blastocysts (> 90%) normally expressed Cdx2. Thus, even though somatic cloned embryos have reduced potential to produce the inner cell mass lineage, the TE lineage can be established and maintained.
authors.affiliation: Laboratory for Genomic Reprogramming, Center for Developmental Biology RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Kobe 650-0047, Japan. kiskigami@cdb.riken.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kishigami
authors.forename: Takafusa
authors.initials: T
authors.lastname: Hikichi
authors.forename: Nguyen
authors.initials: N
authors.lastname: Van Thuan
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohta
authors.forename: Sayaka
authors.initials: S
authors.lastname: Wakayama
authors.forename: Hong-Thuy
authors.initials: HT
authors.lastname: Bui
authors.forename: Eiji
authors.initials: E
authors.lastname: Mizutani
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Wakayama
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D000071616:CDX2 Transcription Factor; D018929:Cell Culture Techniques; D019070:Cell Lineage; D002467:Cell Nucleus; D002999:Clone Cells / Q000166:cytology; D004622:Embryo, Mammalian / Q000166:cytology*; D018398:Homeodomain Proteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D050814:Octamer Transcription Factor-3 / Q000032:analysis; D013234:Stem Cells / Q000166:cytology; D014157:Transcription Factors / Q000032:analysis
chemical_list: D000071616:CDX2 Transcription Factor; C487514:Cdx2 protein, mouse; D018398:Homeodomain Proteins; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D014157:Transcription Factors