Antibacterial effects of human group IIA and group XIIA phospholipase A2 against Helicobacter pylori in vitro.
Group IIA phospholipase A2 (PLA2-IIA) is an enzyme which has important roles in inflammation and infection. Recently, a novel human secretory PLA2 called group XIIA PLA2 (PLA2-XIIA) has been identified. Both PLA2-IIA and PLA2-XIIA are bactericidal against Gram-positive bacteria like many other secretory PLA2s. However, PLA2-XIIA is the only known PLA2 displaying significant bactericidal activity against the Gram-negative bacterium Escherichia coli. We examined the antibacterial properties of recombinant human PLA2-IIA and PLA2-XIIA against Helicobacter pylori, a Gram-negative bacterium, in vitro. PLA2-IIA was not bactericidal against H. pylori, whereas PLA2-XIIA effectively killed H. pylori at a concentration of 50 microg/ml but was not bactericidal at concentrations of 0.5 microg/ml and 5 microg/ml.
Huhtinen
Heikki T
HT
Department of Surgery, University of Turku, Finland. rajaa_ezzahiri@hotmail.com
Grönroos
Juha O
JO
Grönroos
Juha M
JM
Uksila
Jaakko
J
Gelb
Michael H
MH
Nevalainen
Timo J
TJ
Laine
V Jukka O
VJ
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D054501:Group II Phospholipases A2; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000254:growth & development*; D006801:Humans; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D011994:Recombinant Proteins / Q000494:pharmacology; D013272:Stomach Diseases / Q000188:drug therapy* / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2