Application of optical isomer analysis by diastereomer derivatization GC/MS to determine the condition of patients with short bowel syndrome.
To establish a method for separating the optical isomers of lactic acid, we modified the derivatization steps in our procedure for urinary mass-screening for inborn errors of metabolism. For chiral recognition, we chose O-trifluoroacetyl-(-)-menthylation derivatization instead of our previous method, trimethylsilyl derivatization, and the samples were then analyzed under GC/MS by capillary gas chromatography on a DB-5MS column. This method can be used to follow-up the condition of a patient with short bowel syndrome and to prevent onset and/or seizure. d-Lactic acid was also isolated from the urine of healthy controls as one of the main peaks in the chromatogram.
Inoue
Yoshito
Y
Division of Human Genetics, Medical Research Institute, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Ishikawa 9200293, Japan. yosh@kanazawa-med.ac.jp
Shinka
Toshihiro
T
Ohse
Morimasa
M
Ikawa
Hiromichi
H
Kuhara
Tomiko
T
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D019344:Lactic Acid / Q000032:analysis* / Q000737:chemistry; D008297:Male; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012778:Short Bowel Syndrome / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D013237:Stereoisomerism
D019344:Lactic Acid