Assessments of the accumulation severities of amyloid beta-protein and hyperphosphorylated tau in the medial temporal cortex of control and Alzheimer's brains.
Alzheimer's disease (AD) is characterized neuropathologically by neuritic plaques (NPs), and neurofibrillary tangles (NFTs). So far, the following key issues are not yet answered to the disease: (1) the accumulation degrees of three Abeta variants, and tau phosphorylation epitopes in AD as compared to control; (2) the correlation degrees of levels of three Abeta variants with different tau phosphorylation epitopes; (3) the correlation degrees of levels of three Abeta variants and different tau phosphorylation epitopes with Braak and CERAD staging systems. To address these issues, levels of Abeta40, Abeta42, and Abeta43, and phosphorylated tau were assessed by dot blots in homogenates of the medial temporal cortex from AD and control brains in the present study. These data implied different roles of tau phosphorylation epitopes in formation of NFTs, and in this process, Abeta might play a key role. Assessments of levels of these abnormal proteins by dot blots may serve as a useful complement to the morphological evaluations in diagnosis of AD.
Zhou
Xin-Wen
XW
Karolinska Institutet, Department of Neurotec, Geriatric-lab, Novum Plan 5, S-14157, Huddinge, Sweden.
Li
Xu
X
Bjorkdahl
Cecilia
C
Sjogren
Magnus J
MJ
Alafuzoff
Irina
I
Soininen
Hilkka
H
Grundke-Iqbal
Inge
I
Iqbal
Khalid
K
Winblad
Bengt
B
Pei
Jin-Jing
JJ
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D001923:Brain Chemistry / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008297:Male; D016874:Neurofibrillary Tangles / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism*; D010766:Phosphorylation; D013702:Temporal Lobe / Q000378:metabolism / Q000473:pathology*; D025801:Ubiquitin / Q000378:metabolism; D016875:tau Proteins / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D000939:Epitopes; D010446:Peptide Fragments; D025801:Ubiquitin; D016875:tau Proteins