title: From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
abstract: Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal lymphoid tissues. We propose that genome-based, affinity proteomics, using protein epitope signature tag-induced antibodies, is an efficient way to rapidly identify a number of disease-associated protein candidates of both previously known and unknown identities.
authors.affiliation: Department of Immunotechnology, Lund University, SE-22007 Lund, Sweden. sara.ek@immun.lth.se
authors.forename: Sara
authors.initials: S
authors.lastname: Ek
authors.forename: Ulrika
authors.initials: U
authors.lastname: Andréasson
authors.forename: Sophia
authors.initials: S
authors.lastname: Hober
authors.forename: Caroline
authors.initials: C
authors.lastname: Kampf
authors.forename: Fredrik
authors.initials: F
authors.lastname: Pontén
authors.forename: Mathias
authors.initials: M
authors.lastname: Uhlén
authors.forename: Hartmut
authors.initials: H
authors.lastname: Merz
authors.forename: Carl A K
authors.initials: CA
authors.lastname: Borrebaeck
mesh_terms: D000912:Antibodies, Neoplasm; D016022:Case-Control Studies; D002648:Child; D000939:Epitopes; D018985:Epitopes, B-Lymphocyte; D006801:Humans; D008221:Lymphoid Tissue / Q000737:chemistry / Q000166:cytology; D020522:Lymphoma, Mantle-Cell / Q000737:chemistry* / Q000235:genetics* / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D040901:Proteomics / Q000379:methods*; D046888:Tissue Array Analysis
chemical_list: D000912:Antibodies, Neoplasm; D000939:Epitopes; D018985:Epitopes, B-Lymphocyte; D009363:Neoplasm Proteins