title: Proteomic analysis of the tegument and excretory-secretory products of adult Schistosoma bovis worms.
abstract: Schistosoma bovis is a ruminant pathogen that is poorly known at a molecular level. With an aim of identifying the parasite proteins involved in host-parasite interplay, we studied two protein extracts that contain, respectively, the proteins excreted/secreted by the adult worm (ES) and the tegumental proteins exposed to the host (TG). The 2-DE, 2-D immunoblot and MS were employed to separate and identify the antigenic proteins and the most abundant non-antigenic proteins in each extract. There were some 400 and 600 spots detected in the ES and the TG extracts, respectively. Ninety-six spots were subjected to MS analysis and 64 of them were identified. Overall, we identified 18 S. bovis proteins located at the host-parasite interface, 16 of which have not been identified previously in this parasite, and one of which -lysozyme- has never been reported in a Schistosoma species. Of the proteins identified, at least 4 can counteract host defence mechanisms. The other proteins are also likely to play some role in the host-parasite relationships. Therefore, studies in grater depth on all these proteins will provide a better understanding of how this parasite interacts with its host and new strategies for anti-schistosome drug or vaccine design.
authors.affiliation: Unidad de Patología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (CSIC), Salamanca, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Pérez-Sánchez
authors.forename: Alicia
authors.initials: A
authors.lastname: Ramajo-Hernández
authors.forename: Vicente
authors.initials: V
authors.lastname: Ramajo-Martín
authors.forename: Ana
authors.initials: A
authors.lastname: Oleaga
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D020543:Proteome / Q000276:immunology / Q000378:metabolism*; D040901:Proteomics; D012547:Schistosoma / Q000276:immunology / Q000378:metabolism*; D012552:Schistosomiasis / Q000276:immunology / Q000378:metabolism; D012756:Sheep / Q000469:parasitology; D012867:Skin / Q000276:immunology / Q000378:metabolism*; D012908:Snails / Q000469:parasitology
chemical_list: D020543:Proteome