title: Sensitive line probe assay that simultaneously detects mutations conveying resistance to lamivudine and adefovir.
abstract: The INNO-LiPA HBV DR v2 assay is designed to detect hepatitis B virus mutations conveying resistance to lamivudine and adefovir. Our study confirms that this assay can simultaneously detect the presence of lamivudine and adefovir resistance mutations in clinical samples, has a high degree of concordance with sequencing, and can detect mutants earlier.
authors.affiliation: Division of Gastroenterology, University of Michigan Medical Center, 1500 East Medical Center Drive, 3912 Taubman Center, Box 0362, Ann Arbor, Michigan 48109-0362, USA.
authors.forename: Munira
authors.initials: M
authors.lastname: Hussain
authors.forename: Scott
authors.initials: S
authors.lastname: Fung
authors.forename: Evelien
authors.initials: E
authors.lastname: Libbrecht
authors.forename: Erwin
authors.initials: E
authors.lastname: Sablon
authors.forename: Carmela
authors.initials: C
authors.lastname: Cursaro
authors.forename: Pietro
authors.initials: P
authors.lastname: Andreone
authors.forename: Anna S F
authors.initials: AS
authors.lastname: Lok
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D024882:Drug Resistance, Viral / Q000235:genetics; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000188:drug therapy / Q000821:virology; D006801:Humans; D066298:In Vitro Techniques; D019259:Lamivudine / Q000494:pharmacology*; D009154:Mutation; D063065:Organophosphonates / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
chemical_list: D000998:Antiviral Agents; D015342:DNA Probes; D004279:DNA, Viral; D063065:Organophosphonates; D019259:Lamivudine; C053001:adefovir; D012194:RNA-Directed DNA Polymerase; D000225:Adenine