Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes.
We have developed spotted cell microarrays for measuring cellular phenotypes on a large scale. Collections of cells are printed, stained for subcellular features, then imaged via automated, high-throughput microscopy, allowing systematic phenotypic characterization. We used this technology to identify genes involved in the response of yeast to mating pheromone. Besides morphology assays, cell microarrays should be valuable for high-throughput in situ hybridization and immunoassays, enabling new classes of genetic assays based on cell imaging.
Narayanaswamy
Rammohan
R
Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, 2500 Speedway, University of Texas, Austin, TX 78712, USA.
Niu
Wei
W
Scouras
Alexander D
AD
Hart
G Traver
GT
Davies
Jonathan
J
Ellington
Andrew D
AD
Iyer
Vishwanath R
VR
Marcotte
Edward M
EM
D020869:Gene Expression Profiling; D005800:Genes, Fungal / Q000235:genetics; D016681:Genome, Fungal; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D010675:Pheromones / Q000235:genetics*; D051964:Receptors, Mating Factor / Q000235:genetics*; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
D010675:Pheromones; D051964:Receptors, Mating Factor; D029701:Saccharomyces cerevisiae Proteins