Crystallization and preliminary X-ray characterization of a ferritin from the hyperthermophilic archaeon and anaerobe Pyrococcus furiosus.
Crystals of the title protein have been produced and preliminary structural analysis has been carried out. The crystals belong to the orthorhombic space group C222(1), with unit-cell parameters a = 258.1, b = 340.1, c = 266.5 A. The protein forms a 24-mer of 20 kDa subunits, which assemble with 432 non-crystallographic symmetry. A total of 36 monomers are found in the asymmetric unit, corresponding to one and a half 24-mers.
Matias
Pedro M
PM
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, EAN, 2784-505 Oeiras, Portugal. matias@itqb.unl.pt
Tatur
Jana
J
Carrondo
Maria Arménia
MA
Hagen
Wilfred R
WR
D001105:Archaea / Q000201:enzymology*; D002478:Cells, Cultured; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005293:Ferritins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry; D020027:Pyrococcus furiosus / Q000201:enzymology* / Q000378:metabolism; D013696:Temperature
D021122:Protein Subunits; D005293:Ferritins; D007501:Iron