Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy.
In order to measure the cycling of a few ( approximately 6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads.
Borejdo
J
J
Department of Molecular Biology and Immunology, the University of North Texas HSC, Fort Worth, TX 76107, USA. jborejdo@hsc.unt.edu
Talent
J
J
Akopova
I
I
Burghardt
T P
TP
D000199:Actins / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000378:metabolism; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000031:analogs & derivatives / Q000378:metabolism; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D007537:Isometric Contraction; D007700:Kinetics; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D020409:Molecular Motor Proteins / Q000502:physiology* / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000502:physiology* / Q000648:ultrastructure; D009210:Myofibrils / Q000201:enzymology / Q000378:metabolism; D009218:Myosins / Q000378:metabolism* / Q000648:ultrastructure; D012235:Rhodamines; D012399:Rotation
D000199:Actins; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D020409:Molecular Motor Proteins; D012235:Rhodamines; C041829:1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide methiodide; D000251:Adenosine Triphosphatases; D009218:Myosins; D005022:Ethyldimethylaminopropyl Carbodiimide