title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapB (Rv2773c) from Mycobacterium tuberculosis.
abstract: Dihydrodipicolinate reductase from Mycobacterium tuberculosis (DapB, DHDPR, Rv2773c) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in three different crystal forms. Preliminary diffraction data analysis suggests the presence of two tetramers in the asymmetric unit of one crystal form and half a tetramer in the other two crystal forms.
authors.affiliation: EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany.
authors.forename: Georgia
authors.initials: G
authors.lastname: Kefala
authors.forename: Robert
authors.initials: R
authors.lastname: Janowski
authors.forename: Santosh
authors.initials: S
authors.lastname: Panjikar
authors.forename: Christoph
authors.initials: C
authors.lastname: Mueller-Dieckmann
authors.forename: Manfred S
authors.initials: MS
authors.lastname: Weiss
mesh_terms: D016880:Anisotropy; D002845:Chromatography; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D050746:Dihydrodipicolinate Reductase / Q000737:chemistry* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D009841:Oligonucleotides; D050746:Dihydrodipicolinate Reductase