title: Coenzyme Q is irreplaceable by demethoxy-coenzyme Q in plasma membrane of Caenorhabditis elegans.
abstract: A procedure was developed to isolate fractions enriched in plasma membrane from Caenorhabditis elegans. Coenzyme Q9 (Q9) was found in plasma membrane isolated from either wild-type or long-lived qm30 and qm51 clk-1 mutant strains of Caenorhabditis elegans, along with dietary coenzyme Q8 (Q8) and the biosynthetic intermediate demethoxy-Q9 (DMQ9). NADH was able to reduce both Q8 and Q9, but not DMQ9. Our results indicate that DMQ9 cannot achieve the same redox role of Q9 in plasma membrane, suggesting that proportion of all these Q isoforms in plasma membrane must be an important factor in establishing the clk-1 mutant phenotype.
authors.affiliation: Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide, Ctra. de Utrera, km 1, E-41013 Sevilla, Spain. aarrluq@upo.es
authors.forename: Antonio
authors.initials: A
authors.lastname: Arroyo
authors.forename: Carlos
authors.initials: C
authors.lastname: Santos-Ocaña
authors.forename: Macarena
authors.initials: M
authors.lastname: Ruiz-Ferrer
authors.forename: Sergio
authors.initials: S
authors.lastname: Padilla
authors.forename: Angela
authors.initials: A
authors.lastname: Gavilán
authors.forename: Juan C
authors.initials: JC
authors.lastname: Rodríguez-Aguilera
authors.forename: Plácido
authors.initials: P
authors.lastname: Navas
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry / Q000648:ultrastructure*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D002462:Cell Membrane / Q000737:chemistry*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism*
chemical_list: C104339:CLK-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; C510543:demethoxy-coenzyme Q9; D009243:NAD; D014451:Ubiquinone; C030778:ubiquinone 8; C030571:ubiquinone 9