title: Linseed based saliva substitutes and their effect on mineral dissolution of predemineralized bovine dentin in vitro.
abstract: OBJECTIVE
  Polymers used in saliva substitutes might inhibit remineralization. The present study evaluated the effects of various calcium, phosphate, and fluoride concentrations of linseed based solutions on the mineral loss of predemineralized bovine dentin in vitro.
  
  
  METHODS
  Dentin specimens were prepared, embedded and polished. The specimens were demineralized (pH 5.6; 6 d; 37 degrees C) and half of each sample was covered with nail varnish. Subsequently, the specimens (n=10) were exposed to 14 linseed based solutions (calcium 0-1.1 mM; phosphate 0-7.7 mM, fluoride 0-0.03 mM) at pH 5.5 and 6.5 as well as to Salinum for 14 days (37 degrees C). The differences in mineral loss (DeltaDeltaZ) between the values prior to and after storage in the solutions were evaluated from microradiographs of thin sections.
  
  
  RESULTS
  After the exposure to the various solutions decreased mineral contents compared to those after demineralization could be observed (p<0.05; paired t-test) whereas for the specimens stored in solution with the highest calcium and phosphate concentration no significant change in mineral loss could be evaluated (p>0.05). Salinum induced a significantly greater mineral loss compared to the equivalent saturated solution (p<0.05; ANOVA, Bonferroni). The general linear model revealed a significant dependency for DeltaDeltaZ on 'phosphate' (p=0.000) and 'fluoride' (p=0.001), but not on 'calcium' (p=0.397) and 'pH' (p=0.368).
  
  
  CONCLUSIONS
  The addition of calcium, phosphates, and fluorides seems to have a positive effect on the remineralizing qualities of linseed based saliva substitutes.
authors.affiliation: Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Campus Benjamin Franklin, Charité, Universitätsmedizin Berlin, Germany.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Meyer-Lueckel
authors.forename: Peter
authors.initials: P
authors.lastname: Tschoppe
authors.forename: Andrej M
authors.initials: AM
authors.lastname: Kielbassa
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002130:Calcium Phosphates / Q000494:pharmacology / Q000627:therapeutic use; D002417:Cattle; D003804:Dentin / Q000187:drug effects*; D004356:Drug Storage; D019597:Flax / Q000737:chemistry; D005459:Fluorides / Q000494:pharmacology / Q000627:therapeutic use; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008043:Linseed Oil / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D010936:Plant Extracts / Q000494:pharmacology; D012464:Saliva, Artificial / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D017001:Tooth Demineralization / Q000628:therapy; D014089:Tooth Remineralization / Q000379:methods*
chemical_list: D002130:Calcium Phosphates; D010936:Plant Extracts; C450899:Salinum; D012464:Saliva, Artificial; D008043:Linseed Oil; C020243:calcium phosphate; D005459:Fluorides