title: Establishment and characterization of three immortal bovine muscular epithelial cell lines.
abstract: We have established three immortal bovine muscular epithelial (BME) cell lines, one spontaneously immortalized (BMES), the second SV40LT-mediated (BMEV) and the third hTERT-mediated (BMET). The morphology of the three immortal cell lines was similar to that of early passage primary BME cells. Each of the immortal cell lines made cytokeratin, a typical epithelial marker. BMET grew faster than the other immortal lines and the BME cells, in 10% FBS-DMEM medium, whereas neither the primary cells nor the three immortal cell lines grew in 0.5% FBS-DMEM. The primary BME cells and the immortal cell lines, with the exception of BMES, made increasing amounts of p53 protein when treated with doxorubicin, a DNA damaging agent. On the other hand, almost half of the cells in populations of the three immortal cell lines may lack p16(INK4a) regulatory function, compared to primary BME cells that were growth arrested by enforced expression of p16(INK4a). In soft-agar assays, the primary cells and immortal cell lines proved to be less transformed in phenotype than HeLa cells. The three immortal epithelial-type cell lines reported here are the first cell lines established from muscle tissue of bovine or other species.
authors.affiliation: The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University, Seoul 136-701, Korea.
authors.forename: Xun
authors.initials: X
authors.lastname: Jin
authors.forename: Joong-Seob
authors.initials: JS
authors.lastname: Lee
authors.forename: Sungwook
authors.initials: S
authors.lastname: Kwak
authors.forename: Soo-Yeon
authors.initials: SY
authors.lastname: Lee
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Jung
authors.forename: Tae-Kyung
authors.initials: TK
authors.lastname: Kim
authors.forename: Chenxiong
authors.initials: C
authors.lastname: Xu
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Hong
authors.forename: Zhehu
authors.initials: Z
authors.lastname: Li
authors.forename: Sun-Myung
authors.initials: SM
authors.lastname: Kim
authors.forename: Xumin
authors.initials: X
authors.lastname: Pian
authors.forename: Dong-Hee
authors.initials: DH
authors.lastname: Lee
authors.forename: Jong-Taek
authors.initials: JT
authors.lastname: Yoon
authors.forename: Seungkwon
authors.initials: S
authors.lastname: You
authors.forename: Yun-Jaie
authors.initials: YJ
authors.lastname: Choi
authors.forename: Huunggee
authors.initials: H
authors.lastname: Kim
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002460:Cell Line; D002461:Cell Line, Transformed; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D007700:Kinetics; D009132:Muscles / Q000166:cytology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D016159:Tumor Suppressor Protein p53