Measurement of action spectra of light-activated processes.
We report on a new experimental technique suitable for measurement of light-activated processes, such as fluorophore transport. The usefulness of this technique is derived from its capacity to decouple the imaging and activation processes, allowing fluorescent imaging of fluorophore transport at a convenient activation wavelength. We demonstrate the efficiency of this new technique in determination of the action spectrum of the light mediated transport of rhodamine 123 into the parasitic protozoan Giardia duodenalis.
Ross
Justin A
JA
University of Queensland, Centre for Biophotonics and Laser Science, School of Physical Sciences, Saint Lucia, 4072 Queensland, Australia. justinr@physics.uq.edu.au
Zvyagin
Andrei V
AV
Heckenberg
Norman R
NR
Upcroft
Jacqui
J
Upcroft
Peter
P
Rubinsztein-Dunlop
Halina
H
D000818:Animals; D001693:Biological Transport, Active / Q000528:radiation effects; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005872:Giardia / Q000378:metabolism* / Q000528:radiation effects; D008027:Light; D008565:Membrane Proteins / Q000378:metabolism*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D020112:Rhodamine 123 / Q000493:pharmacokinetics*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D008565:Membrane Proteins; D020112:Rhodamine 123