title: Hemoglobin effects in the Saville assay.
abstract: There is a great need to establish accurate, sensitive methods for measuring the concentration of nitrosothiols. Although some progress may have been made recently, differing methodologies have lead to reports of basal levels of nitrosothiols in human plasma that differ by three orders of magnitude. The Saville assay has been widely accepted as an accurate method for measuring nitrosothiols, but one that suffers from sensitivity below that of some other methods. Recently, it has been suggested that when hemoglobin is included in reaction mixtures used for the Saville assay, the sensitivity can be increased by an order of magnitude. Here we show that, on the contrary, the presence of sufficient hemoglobin in the Saville assay decreases its sensitivity.
authors.affiliation: Department of Physics, Wake Forest University, Winston-Salem, NC 27109-7507, USA.
authors.forename: Swati
authors.initials: S
authors.lastname: Basu
authors.forename: Jared D
authors.initials: JD
authors.lastname: Hill
authors.forename: Howard
authors.initials: H
authors.lastname: Shields
authors.forename: Jinming
authors.initials: J
authors.lastname: Huang
authors.forename: S
authors.initials: S
authors.lastname: Bruce King
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Kim-Shapiro
mesh_terms: D001391:Azo Compounds; D006454:Hemoglobins; D026422:S-Nitrosoglutathione / Q000032:analysis*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods
chemical_list: D001391:Azo Compounds; D006454:Hemoglobins; D026422:S-Nitrosoglutathione