title: Cellulose-based scaffold materials for cartilage tissue engineering.
abstract: Non-woven cellulose II fabrics were used as scaffolds for in vitro cartilage tissue engineering. The scaffolds were activated in a saturated Ca(OH)(2) solution and subsequently coated with a calcium phosphate layer precipitated from a supersaturated physiological solution. Chondrocyte cell response and cartilage development were investigated. The cell adherence was significantly improved compared to untreated cellulose fabrics, and the proliferation and vitality of the adhered chondrocytes were excellent, indicating the biocompatibility of these materials. A homogeneous distribution of the seeded cells was possible and the development of cartilageous tissue could be proved. In contact with a physiological chondrocyte solution, calcium is expected to be leached out from the precipitated layer, which might lead to a microenvironment that triggers the development of cartilage in a way similar to cartilage repair in the vicinity of subchondral bone.
authors.affiliation: Department of Materials Science-Biomaterials, University of Erlangen-Nuernberg, Henkestr. 91, 91052 Erlangen, Germany. Frank.Mueller@ww.uni-erlangen.de
authors.forename: Frank A
authors.initials: FA
authors.lastname: Müller
authors.forename: Lenka
authors.initials: L
authors.lastname: Müller
authors.forename: Ingo
authors.initials: I
authors.lastname: Hofmann
authors.forename: Peter
authors.initials: P
authors.lastname: Greil
authors.forename: Magdalene M
authors.initials: MM
authors.lastname: Wenzel
authors.forename: Rainer
authors.initials: R
authors.lastname: Staudenmaier
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002356:Cartilage / Q000166:cytology* / Q000254:growth & development*; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002482:Cellulose / Q000737:chemistry*; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry; D008422:Materials Testing; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D002482:Cellulose