Reinfections with strains of Trypanosoma cruzi, of different biodemes as a factor of aggravation of myocarditis and myositis in mice.
Reinfections with Trypanosoma cruzi in patients from endemic areas have been claimed to be an aggravation factor of cardiac manifestations in Chagas' disease. In the present study, the influence of triple infections with strains of different biodemes, on cardiac and skeletal muscle lesions was experimentally tested. Fifty eight mice chronically infected with the Colombian strain (Biodeme Type III) were successively reinfected as follows: 1st group--reinfected with 21 SF strain (Type II) followed by Y strain (Type I ); 2nd--group reinfections with Y strain followed by 21SF strain. Isoenzyme analysis of parasites from hemocultures obtained from triple infected mice, revealed the patterns of three distinct zymodemes in the same animal. Each Trypanosoma cruzi strain was reisolated after four passages in mice on either the 7th, 14th or 30th day after inoculation with the blood of triple infected mice. Histopathology results demonstrated a significant exacerbation of cardiac and skeletal muscle inflammatory lesions, confirmed by morphometric evaluation, in mice with triple infection. No aggravation of parasitism was detected. The possibility of an enhancement of cellular response in the triple infected mice is suggested.
Andrade
Sonia Gumes
SG
Laboratório de Chagas Experimental, Autoimunidade e Imunologia Celular, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA. sgandrade@cpqgm.fiocruz.br
Campos
Rozália Figueira
RF
Sobral
Karina Souza Castro
KS
Magalhães
Juracy Barbosa
JB
Guedes
Ricardo S Pereira
RS
Guerreiro
Marcos Lázaro
ML
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology / Q000473:pathology*; D007527:Isoenzymes / Q000032:analysis; D051379:Mice; D009205:Myocarditis / Q000469:parasitology* / Q000473:pathology; D009220:Myositis / Q000469:parasitology* / Q000473:pathology; D018512:Parasitemia / Q000473:pathology; D013045:Species Specificity; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000145:classification* / Q000201:enzymology / Q000472:pathogenicity
D007527:Isoenzymes