title: Molecular analysis of Fiji disease virus segments 2, 4 and 7 completes the genome sequence.
abstract: The complete nucleotide sequences of Fiji disease virus (FDV) genome segments S2, S4 and S7 were determined. This now completes the sequencing of all ten dsRNA genome segments of the Fijivirus type member, FDV, which comprises a total of 29339 nt. FDV S2, S4 and S7 comprised 3820, 3568 and 2194 nt, respectively. S2 and S4 each contained a single open reading frame (ORF), which encoded putative proteins of 137 and 133 kDa, respectively, while S7 contained two ORFs, which encoded putative proteins of 42 and 37 kDa. The putative amino acid sequences of FDV S2 and S4 showed most similarity to the gene products of Rice black-streaked dwarf virus (RBSDV) S2 and RBSDV S3, respectively. The putative amino acid sequences of FDV S7 ORF I and II showed most similarity to Maize rough dwarf virus (MRDV) S6 ORF I and RBSDV S7 ORF II, respectively. Phylogenetic analyses showed that FDV was most closely related to the group 2 fijiviruses.
authors.affiliation: Science Research Centre, Faculty of Science, Queensland University of Technology, 2 George Street, 4000, Brisbane, Qld, Australia. r.harding@qut.edu.au
authors.forename: Robert M
authors.initials: RM
authors.lastname: Harding
authors.forename: Parichart
authors.initials: P
authors.lastname: Burns
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Geijskes
authors.forename: Richard M
authors.initials: RM
authors.lastname: McQualter
authors.forename: James L
authors.initials: JL
authors.lastname: Dale
authors.forename: Grant R
authors.initials: GR
authors.lastname: Smith
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D016679:Genome, Viral; D008970:Molecular Weight; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000145:classification / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D020079:Terminal Repeat Sequences; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins