Macroscopic 2D networks self-assembled from nanometer-sized protein/DNA complexes.
We demonstrate the self-assembly of DNA and DNA binding proteins into two-dimensional networks that are then addressable by sending a second protein to a specific recognition site on the DNA network. These networks cover centimeters in area but can be addressed with nanometer precision. This hierarchical self-assembly of specific DNA protein complexes will be the basis for complex positioning of single molecules in two and three dimensions.
Manzanera
Maximino
M
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
Frankel
Daniel J
DJ
Li
Haitao
H
Zhou
Dejian
D
Bruckbauer
Andreas
A
Kreutzmann
Peter
P
Blackburn
Jonathan M
JM
Abell
Chris
C
Rayment
Trevor
T
Klenerman
David
D
Barker
Paul D
PD
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D016328:NF-kappa B / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D011506:Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*; D013057:Spectrum Analysis
D016328:NF-kappa B; D011506:Proteins; D004247:DNA