[Constructing of STR slippage model by optimizing some factors].
OBJECTIVE
To construct STR slippage model and study factors involved in this procedure.


METHODS
DNA samples were amplified with the technology of Degenerate oligonucleotide- primed PCR, then their products were taken as later DNA template and their STR genotype were analyzed by optimizing several factors.


RESULTS
STR slippage model was constructed.


CONCLUSIONS
Several factors were involved in the produce of STR slippage, such as amount of modulate DNA, concentration of MgCl2, property of DNA polymerase, motif sequence of STR loci, sample, etc.
Deng
Jian-qiang
JQ
Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063,China. forensicpatho@163.com
Li
Ying-bi
YB
Wu
Jin
J
Hou
Yi-ping
YP
D004247:DNA / Q000302:isolation & purification; D017931:DNA Primers; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008274:Magnesium; D008957:Models, Genetic; D016133:Polymerase Chain Reaction / Q000379:methods*; D020080:Tandem Repeat Sequences / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA; D008274:Magnesium