Sophorolipid produced from the new yeast strain Wickerhamiella domercqiae induces apoptosis in H7402 human liver cancer cells.
The effects of sophorolipid on the growth and apoptosis of H7402 human liver cancer cells were investigated. By treatment with sophorolipid, a dose- and time-dependent inhibition of cell proliferation was observed. The cells developed many features of apoptosis, including condensation of chromatin, nuclear fragmentation, and appearance of apoptotic bodies, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling positive cells were stained dark brown. Sophorolipid treatment induced apoptosis in H7402 cells by blocking cell cycle at G1 phase and partly at S phase, activating caspase-3, and increasing Ca2+ concentration in cytoplasm. These findings may suggest a potential use of sophorolipid for liver cancer treatment.
Chen
Jing
J
State Key Lab of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
Song
Xin
X
State Key Lab of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China. songx@sdu.edu.cn.
Zhang
Hui
H
Institute of Developmental Biology, School of Life Science, Shandong University, Jinan, 250100, China.
Qu
Yin-Bo
YB
State Key Lab of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
Miao
Jun-Ying
JY
Institute of Developmental Biology, School of Life Science, Shandong University, Jinan, 250100, China.
D000970:Antineoplastic Agents / Q000378:metabolism* / Q000494:pharmacology*; D017209:Apoptosis; D001203:Ascomycota / Q000378:metabolism; D002118:Calcium / Q000032:analysis; D053148:Caspase 3 / Q000032:analysis; D045744:Cell Line, Tumor; D002470:Cell Survival; D002843:Chromatin / Q000378:metabolism; D003124:Colorimetry; D003593:Cytoplasm / Q000737:chemistry; D053938:DNA Fragmentation; D005470:Fluorometry; D016193:G1 Phase / Q000187:drug effects; D006017:Glycolipids / Q000096:biosynthesis* / Q000494:pharmacology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology*; D046529:Microscopy, Electron, Transmission; D008858:Microscopy, Phase-Contrast; D016196:S Phase / Q000187:drug effects
D000970:Antineoplastic Agents; D002843:Chromatin; D006017:Glycolipids; C067958:16-(2'-O-glucopyranosylglucopyranosyloxy)hexadecanoic acid 1',4''-lactone 6',6''-diacetate; D053148:Caspase 3; D002118:Calcium