title: [Comparison of effects of two kinds of fluid for resuscitation on bacterial translocation and inflammation of small intestine in rats with hemorrhagic shock].
abstract: OBJECTIVE
  To investigate the effects of Ringer's solution (RS) or 6% hydroxyethyl saline (HES) on bacterial translocation and inflammation of the small intestine in rats with hemorrhagic shock.
  
  
  METHODS
  Fifty healthy male SD rats were randomly divided into the sham group (SHA group, n=10), the Ringer's solution group (RS group, n=20) and 6% hydroxyethyl saline group (HES group, n=20). Controlled hemorrhagic shock model was reproduced in RS and HES groups. Bacterial translocation to the liver, the content of tumor necrosis factor-alpha (TNF-alpha) in intestinal tissue, and the myeloperoxidase (MPO) activity in the intestinal tissue were determined and compared among the groups, and the pathologic changes in the small intestine were observed.
  
  
  RESULTS
  The mortality rate, bleeding volume and Chiu's scores were same in HES and RS groups (all P>0.05). Compared to SHA group, bacterial count and TNF-alpha level were increased significantly in HES and RS groups, and they were higher at 1 hour and lower at 24 hours in HES group than those in RS group. Compared to the SHA group, MPO activity increased at 1 hour in RS and HES groups, but no significant difference between the groups was found at 24 hours.
  
  
  CONCLUSIONS
  RS prevents compromise of the intestinal barrier function better than the HES at 1 hour after fluid resuscitation. However, HES seems to be better in protecting the intestinal barrier function compared with RS at 24 hours after fluid resuscitation.
authors.affiliation: The Key Lab of Anesthesiology of Jiangsu Province, The Anesthesiology Institute of Xuzhou Medical College, Xuzhou 221002, Jiangsu, China. gaodays@163.com
authors.forename: Xin-yue
authors.initials: XY
authors.lastname: Gao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
authors.forename: Chang-yi
authors.initials: CY
authors.lastname: Wu
authors.forename: Qing-feng
authors.initials: QF
authors.lastname: Pang
authors.forename: Jun-li
authors.initials: JL
authors.lastname: Cao
authors.forename: Yin-ming
authors.initials: YM
authors.lastname: Zeng
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D004195:Disease Models, Animal; D005440:Fluid Therapy / Q000379:methods*; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology*; D007421:Intestine, Small / Q000187:drug effects / Q000473:pathology; D007552:Isotonic Solutions / Q000494:pharmacology*; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077331:Ringer's Solution; D012771:Shock, Hemorrhagic / Q000382:microbiology / Q000473:pathology / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D006895:Hydroxyethyl Starch Derivatives; D007552:Isotonic Solutions; D014409:Tumor Necrosis Factor-alpha; D000077331:Ringer's Solution; D009195:Peroxidase