title: Mechanism underlying specificity of proteins targeting inorganic materials.
abstract: Adhesion force analysis using atomic force microscopy clearly revealed for the first time the mechanism underlying the specific binding between a titanium surface and ferritin possessing the sequence of Ti-binding peptide in its N-terminal domain. Our results proved that the specific binding is due to double electrostatic bonds between charged residue and surface groups of the substrate. Furthermore, it is also demonstrated that the accretion of surfactant reduces nonspecific interactions, dramatically enhancing the selectivity and specificity of Ti-binding peptide.
authors.affiliation: Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Yokohama, Kanagawa 226-8502, Japan. tomo_hayashi@mac.com
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Hayashi
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Sano
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Shiba
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kumashiro
authors.forename: Kenji
authors.initials: K
authors.lastname: Iwahori
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yamashita
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hara
mesh_terms: D000595:Amino Acid Sequence; D006046:Gold; D007287:Inorganic Chemicals / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity; D055672:Static Electricity; D000267:Tissue Adhesions
chemical_list: D007287:Inorganic Chemicals; D011506:Proteins; D006046:Gold