title: Detection of a novel variant bla(CTX-M-3) extended spectrum beta-lactamase gene in a community-acquired Escherichia coli isolate.
abstract: A highly cefotaxime- and cefepime-resistant but ceftazidime-sensitive Escherichia coli isolate was recovered from a community-acquired urinary infection of a Greek patient. Susceptibility testing, transfer assays, plasmid analysis as well as PCR and sequencing techniques were used to investigate the underlying mechanism of resistance. The isolate carried a new variant of the bla(CTX-M-3) gene that possessed a T instead of A at nt position 663. Cefotaxime resistance was transferable and carried on a 60 kb plasmid. The bla(CTX-M-3) variant was located downstream of an ISEcp1B element. The emergence of this new derivative indicates further evolution of the worldwide-distributed bla(CTX-M-3) gene.
authors.affiliation: Department of Medical Microbiology, University of Thessalia, Mezourlo, Larissa, Greece.
authors.forename: Spyros
authors.initials: S
authors.lastname: Pournaras
authors.forename: Athanassios
authors.initials: A
authors.lastname: Tsakris
authors.forename: Alexandros
authors.initials: A
authors.lastname: Ikonomidis
authors.forename: Antonios
authors.initials: A
authors.lastname: Markogiannakis
authors.forename: Ioulia
authors.initials: I
authors.lastname: Kristo
authors.forename: Antonios N
authors.initials: AN
authors.lastname: Maniatis
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000077723:Cefepime; D002439:Cefotaxime / Q000494:pharmacology; D018550:Cephalosporin Resistance / Q000235:genetics*; D002511:Cephalosporins / Q000494:pharmacology; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D005260:Female; D014644:Genetic Variation; D006115:Greece; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D014552:Urinary Tract Infections / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D000077723:Cefepime; C417271:beta-lactamase CTX-M-3; D001618:beta-Lactamases; D002439:Cefotaxime