title: Use of natural dye-casein complexes: effect of proteolytic treatment.
abstract: The activity of proteolytic enzymes is commonly measured using casein as a substrate. A modified caseinolysis assay was developed with natural dyes such as juglone, lawsone, berberine, and quercetin for Subtilisin carlsberg, protease type XVI, and trypsin, respectively. The pH dependence and incubation time were determined. K(m), V(max), and k(cat)/K(m) values were also determined for these enzymes. Lawsone was found to be a better substrate than the others.
authors.affiliation: Department of Chemistry, Faculty of Science Art, Dicle University, Diyarbakir, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Doğru
authors.forename: Zübeyde
authors.initials: Z
authors.lastname: Baysal
authors.forename: Cetin
authors.initials: C
authors.lastname: Aytekin
authors.forename: Haluk
authors.initials: H
authors.lastname: Aydin
mesh_terms: D002364:Caseins / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010447:Peptide Hydrolases / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D002364:Caseins; D004396:Coloring Agents; D010447:Peptide Hydrolases