Increased levels of mitochondrial gene transcripts in the thermally selected rainbow trout (Oncorhynchus mykiss) strain during embryonic development.
To investigate molecular mechanisms involved in thermal resistance of rainbow trout, Oncorhynchus mykiss, embryos from thermally selected strain in various developmental stages were treated at 22 degrees C for 30 min and subsequently developed at 12 degrees C using the Donaldson strain as a reference. The embryos were evaluated for their hatching rate along with the ratio of embryos having an abnormal appearance and subjected to mRNA arbitrarily primed reverse transcription-polymerase chain reaction (RAP RT-PCR). One of the genes dominantly expressed in the thermally selected strain (COX II) coded for cytochrome c oxidase subunit II. Northern blot analysis revealed that the accumulated levels of COX II transcripts were more abundant in embryos and unfertilized eggs from the thermally selected strain than those from the Donaldson strain. Furthermore, the differential expression patterns of the ATPase 6-8 gene were similar to those of the COX II gene, whereas the ATP synthase beta-subunit gene showed no significant differences between the two strains.
Ikeguchi
Koki
K
Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.
Ineno
Toshinao
T
Itoi
Shiro
S
Kondo
Hidehiro
H
Kinoshita
Shigeharu
S
Watabe
Shugo
S
D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004527:Egg Proteins / Q000032:analysis / Q000502:physiology; D003576:Electron Transport Complex IV / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D023061:Gene Order; D050259:Genes, Mitochondrial / Q000502:physiology*; D018882:Heat Stress Disorders / Q000401:mortality / Q000662:veterinary; D006358:Hot Temperature; D025261:Mitochondrial Proton-Translocating ATPases / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000196:embryology* / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment
C506526:ATP5F1B protein, human; D004527:Egg Proteins; C117108:cytochrome C oxidase subunit II; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases