Human and chicken antibodies to gangliosides following infection by Campylobacter jejuni.
Campylobacteriosis is frequently associated with Guillain-Barré syndrome. Poultry are frequently highly colonized with Campylobacter jejuni and are a major foodborne vehicle for campylobacteriosis. In this study, high titer anti-GM1 antibodies were found in the serum of a laboratory worker who developed campylobacteriosis. The microbiologically confirmed strain VLA2/18 (non-serotyped) was isolated from the worker and subsequently inoculated into chickens, resulting in high titers of serum antibodies to GM1. However, none of the immunized chickens in our study showed any noticeable neurological symptoms, such as paralysis or cramping. High titer anti-GM1 antibodies in chicken and human sera strongly inhibited spontaneous muscle action potential in an in vitro system of spinal cord and muscle cell co-culture. In addition, infection of chickens with C. jejuni strains 81116 (HS6) and 99/419 (HS21) or immunization with purified GM1, GM2, and GM3 resulted in elevation of serum anti-ganglioside antibodies with an inhibitory effect on spontaneous muscle action potential. Immunoabsorption studies demonstrated that this inhibitory activity is due to anti-ganglioside antibodies. On the other hand, anti-GM1 is the only specific human serum antibody to induce an inhibitory effect on neuromuscular junctions. Chicken anti-GM1 antibodies showed a strong inhibitory effect, but anti-GM2 and -GM3 had weaker activities. Taken together, our data suggest that campylobacteriosis in chickens may provide a strong link between infection and the development of anti-ganglioside antibody-mediated peripheral nerve dysfunctions.
Usuki
Seigo
S
Institute of Molecular Medicine and Genetics and Institute of Neuroscience, Medical College of Georgia, Augusta, GA 30912-2697, USA.
Taguchi
Kyoji
K
Cawthraw
Shaun A
SA
Shibata
Keiko
K
Ariga
Toshio
T
Newell
Diane G
DG
Yu
Robert K
RK
D000200:Action Potentials / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood*; D002169:Campylobacter Infections / Q000276:immunology* / Q000517:prevention & control; D016123:Campylobacter jejuni / Q000276:immunology* / Q000302:isolation & purification; D002645:Chickens; D018920:Coculture Techniques; D005732:Gangliosides / Q000276:immunology*; D006801:Humans; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control; D051381:Rats; D013045:Species Specificity
D000907:Antibodies, Bacterial; D005732:Gangliosides