title: Laboratory colonization of Anopheles aquasalis (Diptera: Culicidae) in Belém, Pará, Brazil.
abstract: Anopheles aquasalis Curry, a coastal malaria vector with a Neotropical distribution, was collected from Belém, Pará state, Brazil, and 500 adults per cage were maintained at the Instituto Evandro Chagas insectary at 26 -30 degrees C and 80 -90% RH, where they fed on a 10% domestic sugar solution and blood from white mice. Oviposition of the parental generation (P) occurred in fresh water in dark cups introduced into mosquito cages. After eclosion, 100 larvae per pan were reared in artesian well water (salinity 0.04 g liter(-10) and fed ground fish food until pupation. After force mating in the F1 generation, the eight subsequent generations were free mating. Mean larval mortality was < 1%, and the mean developmental time from eclosion to emergence was 7.7 d (F1), 7.6 d (F2), 8 d (F3), and 7.5 d (F4). The maximum daily production of pupae (from the fourth generation on) occurred on day 6 postoviposition.
authors.affiliation: Programa em Malaria, Seção de Parasitologia do Instituto Evandro Chagas, Belém, Pará, Brazil.
authors.forename: Ana N M
authors.initials: AN
authors.lastname: Da Silva
authors.forename: Carla C B
authors.initials: CC
authors.lastname: Dos Santos
authors.forename: Raimundo N L
authors.initials: RN
authors.lastname: Lacerda
authors.forename: Edvaldo P
authors.initials: EP
authors.lastname: Santa Rosa
authors.forename: Raimundo T L
authors.initials: RT
authors.lastname: De Souza
authors.forename: Deocleciano
authors.initials: D
authors.lastname: Galiza
authors.forename: Izis
authors.initials: I
authors.lastname: Sucupira
authors.forename: Jan E
authors.initials: JE
authors.lastname: Conn
authors.forename: Marinete M
authors.initials: MM
authors.lastname: Póvoa
mesh_terms: D000818:Animals; D000852:Anopheles / Q000254:growth & development* / Q000502:physiology; D001938:Brazil; D001947:Breeding; D002645:Chickens; D006224:Cricetinae; D005260:Female; D007303:Insect Vectors / Q000254:growth & development* / Q000502:physiology; D008297:Male; D051379:Mice; D010058:Oviposition; D011784:Quail