title: Imaging of the three-dimensional alveolar structure and the alveolar mechanics of a ventilated and perfused isolated rabbit lung with Fourier domain optical coherence tomography.
abstract: In this feasibility study, Fourier domain optical coherence tomography (FDOCT) is used for visualizing the 3-D structure of fixated lung parenchyma and to capture real-time cross sectional images of the subpleural alveolar mechanics in a ventilated and perfused isolated rabbit lung. The compact and modular setup of the FDOCT system allows us to image the first 500 microm of subpleural lung parenchyma with a 3-D resolution of 16 x 16 x 8 microm (in air). During mechanical ventilation, real-time cross sectional FDOCT images visualize the inflation and deflation of alveoli and alveolar sacks (acini) in successive images of end-inspiratory and end-expiratory phase. The FDOCT imaging shows the relation of local alveolar mechanics to the setting of tidal volume (VT), peak airway pressure, and positive end-expiratory pressure (PEEP). Application of PEEP leads to persistent recruitment of alveoli and acini in the end-expiratory phase, compared to ventilation without PEEP where alveolar collapse and reinflation are observed. The imaging of alveolar mechanics by FDOCT will help to determine the amount of mechanical stress put on the alveolar walls during tidal ventilation, which is a key factor in understanding the development of ventilator induced lung injury (VILI).
authors.affiliation: Medical Faculty of Technical University Dresden, Clinical Sensoring and Monitoring, Fetscherstrasse 74, 01307 Dresden, Germany. Alexander.popp@mailbox.tu-dresden.de
authors.forename: Alexander
authors.initials: A
authors.lastname: Popp
authors.forename: Martina
authors.initials: M
authors.lastname: Wendel
authors.forename: Lilla
authors.initials: L
authors.lastname: Knels
authors.forename: Thea
authors.initials: T
authors.lastname: Koch
authors.forename: Edmund
authors.initials: E
authors.lastname: Koch
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D005583:Fourier Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D066298:In Vitro Techniques; D010477:Perfusion; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000502:physiology*; D011817:Rabbits; D015203:Reproducibility of Results; D012121:Respiration, Artificial; D015656:Respiratory Mechanics; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods