title: Expression, purification, crystallization and preliminary crystallographic study of a potential metal-dependent hydrolase with cyclase activity from Thermoanaerobacter tengcongensis.
abstract: The putative metal-dependent hydrolase gene TTE1006 from Thermoanaerobacter tengcongensis strain MB4T (T = type strain; Genbank accession No. AE008691) was heterologously expressed in Escherichia coli. The 205-amino-acid gene product was purified and crystallized. The crystal used for data collection belongs to space group P2(1), with unit-cell parameters a = 85.2, b = 62.1, c = 172.4 A, beta = 104.2 degrees. Using a synchrotron-radiation source, the resolution limit of the data reached 1.87 A. Eight molecules were estimated to be present in the asymmetric unit, with a solvent content of 48%. Structure determination is ongoing using the multiple-wavelength anomalous diffraction (MAD) method and also the molecular-replacement (MR) method.
authors.affiliation: State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Sen
authors.initials: S
authors.lastname: Liu
authors.forename: Guangteng
authors.initials: G
authors.lastname: Wu
authors.forename: Qichen
authors.initials: Q
authors.lastname: Huang
authors.forename: Luhua
authors.initials: L
authors.lastname: Lai
authors.forename: Youqi
authors.initials: Y
authors.lastname: Tang
authors.forename: Hideaki
authors.initials: H
authors.lastname: Unno
authors.forename: Masami
authors.initials: M
authors.lastname: Kusunoki
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D003460:Crystallization; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D006867:Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D017356:Synchrotrons; D046968:Thermoanaerobacter / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D006867:Hydrolases