Quantitative analysis of allergenic ingredients in eluate extracted from used denture base resin.
Allergenic ingredients extracted from used denture base resin were quantitatively analysed using a gas chromatograph/mass spectrometer and high performance liquid chromatography. Methyl methacrylate (MMA), hydroquinone (HQ), formaldehyde (FMA), benzoyl peroxide (BPO), benzoic acid (BA) and methyl benzoate (MB) were detected in a eluate from all of the dentures, while ethyleneglycol dimethacrylate (DME) was detected in the eluate from 87 dentures in use for <15 years. MMA, HQ, FMA, BPO and MB showed a decrease in correlation to the period of denture use, but continuing to be evident even after 29 years of use. The MMA showed the highest level of elution and a relatively moderate decrease over time. The elution of BA, on the contrary, showed an increase with the period of denture use up to about 10 years and subsequently reached a plateau. Our results indicate that purported allergens exist in the resin base and can be eluted into the oral cavity, even in patients using an old denture for a period of nearly 30 years.
Mikai
M
M
Division of Removable Prosthodontics and Management of Oral Function, Department of Developmental and Reconstructive Medicine, Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Koike
M
M
Fujii
H
H
D000180:Acrylic Resins / Q000737:chemistry*; D000485:Allergens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003819:Denture Bases; D016379:Denture, Partial, Fixed, Resin-Bonded; D003778:Dentures; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D013997:Time Factors
D000180:Acrylic Resins; D000485:Allergens