A novel approach for improving the productivity of ubiquinone-10 producing strain by low-energy ion beam irradiation.
Agrobacterium tumefaciens ATCC4452 cells were irradiated by nitrogen ion beam, a new mutagen, with energy of 10 keV and fluence ranging from 2.6x10(14) ions/cm2 to 6.5x10(15) ions/cm2. A similar "saddle shape" survival curve due to ion beam irradiation appeared again in this study. Some mutants with high yield of ubiquinone-10 were induced by ion implantation. High mutation rate and wide mutation spectrum were also observed in the experiment. These results suggested that the mutagenic effect of such low-energy ion influx into bacterium cells could result from multiple processes involving direct collision of particles with cytoplasm, nucleolus, and cascade atomic and molecular reactions due to plentiful primary and secondary particles.
Gu
Shao-Bin
SB
Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, P.O. Box 1126, Hefei 230031P, PR China.
Yao
Jian-Ming
JM
Yuan
Qi-Peng
QP
Xue
Pei-Jian
PJ
Zheng
Zhi-Ming
ZM
Wang
Li
L
Yu
Zeng-Liang
ZL
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D007218:Industrial Microbiology / Q000379:methods*; D009154:Mutation; D009584:Nitrogen / Q000737:chemistry; D011839:Radiation, Ionizing; D014451:Ubiquinone / Q000096:biosynthesis* / Q000235:genetics
D014451:Ubiquinone; C025204:Ubiquinone Q2; D009584:Nitrogen