title: Correlation of the turbo-MP RIA with ImmunoCAP FEIA for determination of food allergen-specific immunoglobulin E.
abstract: It has been reported that in vitro measurement of food-specific IgE can be used to accurately predict food allergy and reduce the risk associated with double-blinded placebo-controlled food challenges (DBPCFC). Our objective was to assess the performance characteristics of the Hycor Turbo-MP quantitative radioimmunoassay for food-specific IgE and to determine this method's comparability to another assay, the Pharmacia ImmunoCAP fluorescence enzyme immunoassay (FEIA). The dynamic range of the Turbo-MP assay is 0.05 to 100 IU/ml, compared to 0.35 to 100 IU/ml for the FEIA. Performance characteristics of the Turbo-MP assay (ie, reproducibility of the calibration curve, within-run precision, total precision, parallelism, and linearity) were determined using samples from the Hycor serum bank. The precision (CV) of IgE calibrator replicates was <10%. The total precision (CV) of the Turbo-MP assay ranged from 8.8% to 18.4% for specific IgE concentrations between 0.28 to 31.4 IU/ml. Testing of serial dilutions of sera with IgE specificities for egg white, cow's milk, codfish, wheat, peanut, and soybean showed that the assay is linear over the entire dynamic range. Serial dilution data (slopes of 1.01 to 1.10) showed parallelism to serial dilutions of the IgE calibrator (slope of 0.96). The Turbo-MP and FEIA methods were both used for quantitative assays of food-specific IgE in 457 serum samples obtained from a clinical reference laboratory. Comparison of specific IgE results by the Turbo-MP and FEIA methods for 6 major food allergens exhibited a slope of 0.99 (0.92 to 1.03) with a correlation coefficient of 0.81.
authors.affiliation: Hycor Stratagene, 7272 Chapman Ave., Garden Grove, CA 92841, USA. kkontis@hycorbiomedical.com
authors.forename: Kris J
authors.initials: KJ
authors.lastname: Kontis
authors.forename: Andre
authors.initials: A
authors.lastname: Valcour
authors.forename: Ashok
authors.initials: A
authors.lastname: Patel
authors.forename: Andy
authors.initials: A
authors.lastname: Chen
authors.forename: Jan
authors.initials: J
authors.lastname: Wang
authors.forename: Julia
authors.initials: J
authors.lastname: Chow
authors.forename: Narayan
authors.initials: N
authors.lastname: Nayak
mesh_terms: D000485:Allergens / Q000276:immunology*; D005453:Fluorescence; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000097:blood*; D011852:Radioallergosorbent Test; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000485:Allergens; D007073:Immunoglobulin E