title: Production, purification, crystallization and preliminary X-ray analysis of adeno-associated virus serotype 8.
abstract: Adeno-associated viruses (AAVs) are actively being developed for clinical gene-therapy applications and the efficiencies of the vectors could be significantly improved by a detailed understanding of their viral capsid structures and the structural determinants of their tissue-transduction interactions. AAV8 is approximately 80% identical to the more widely studied AAV2, but its liver-transduction efficiency is significantly greater than that of AAV2 and other serotypes. The production, purification, crystallization and preliminary X-ray crystallographic analysis of AAV8 viral capsids are reported. The crystals diffract X-rays to 3.0 A resolution using synchrotron radiation and belong to the hexagonal space group P6(3)22, with unit-cell parameters a = 257.5, c = 443.5 A. The unit cell contains two viral particles, with ten capsid viral protein monomers per crystallographic asymmetric unit.
authors.affiliation: Department of Biochemistry and Molecular Biology, Center for Structural Biology, McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Michael Douglas
authors.initials: MD
authors.lastname: Lane
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Nam
authors.forename: Eric
authors.initials: E
authors.lastname: Padron
authors.forename: Brittney
authors.initials: B
authors.lastname: Gurda-Whitaker
authors.forename: Eric
authors.initials: E
authors.lastname: Kohlbrenner
authors.forename: George
authors.initials: G
authors.lastname: Aslanidi
authors.forename: Barry
authors.initials: B
authors.lastname: Byrne
authors.forename: Robert
authors.initials: R
authors.lastname: McKenna
authors.forename: Nicholas
authors.initials: N
authors.lastname: Muzyczka
authors.forename: Sergei
authors.initials: S
authors.lastname: Zolotukhin
authors.forename: Mavis
authors.initials: M
authors.lastname: Agbandje-McKenna
mesh_terms: D000818:Animals; D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D003460:Crystallization / Q000379:methods; D000229:Dependovirus / Q000737:chemistry*; D007313:Insecta; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D036022:Capsid Proteins; D014764:Viral Proteins