Incorporation of a disaccharide nucleoside into the backbone of double-stranded DNA: crystallization and preliminary X-ray diffraction.
Incorporation of a disaccharide nucleoside into double-stranded DNA can be considered as a chemical (non-enzymatic) alternative for site-specific cleavage of DNA. Crystals of the sequence d(CGCGAATT*CGCG), where * is an incorporated ribose, were obtained by hanging-drop vapour diffusion and diffracted to 2.6 A. The crystals belong to the orthorhombic space group P222(1), with unit-cell parameters a = 41.52, b = 57.63, c = 81.39 A, indicating a new crystal packing motif for an oligonucleotide dodecamer sequence.
Van Hecke
Kristof
K
Biomolecular Architecture, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, B-3001 Leuven (Heverlee), Belgium.
Uytterhoeven
Koen
K
Mikhailov
Sergey N
SN
Herdewijn
Piet
P
Van Meervelt
Luc
L
D003460:Crystallization; D004247:DNA / Q000737:chemistry*; D004058:Diffusion; D004187:Disaccharides; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D012266:Ribose / Q000737:chemistry; D014961:X-Ray Diffraction
D004187:Disaccharides; D009705:Nucleosides; D009841:Oligonucleotides; D012266:Ribose; D004247:DNA