Determination of aflatoxin B1 in tiger nut-based soft drinks.
An analytical method for the determination of aflatoxin B1 in a tiger nut-based soft drinks named 'horchata' is described. The method is based on an immunoaffinity clean-up, followed by HPLC separation and fluorescence detection after electrochemical post-column derivatization. The detection limit (S/N = 3) and the quantification limit (S/N = 10) were 0.02 and 0.06 microg kg(-1), respectively. The mean recovery at a level of 2 microg l(-1) was 88% (n = 6) and the coefficient of variation was 9%. The method was applied to conduct a small market survey for a beverage named 'horchata' that is frequently consumed by parts of the population in Southern Europe. Twenty-two samples from Spanish and Belgian supermarkets were analysed. As a result, only one sample was found to contain aflatoxin B1 at the limit of quantitation of the method.
Arranz
I
I
Food Safety & Quality Unit, Institute for Reference Materials and Measurements, European Commission - Joint Research Centre, Retieseweg, B-2440 Geel, Belgium. isabel.arranz-hernandez@cec.eu.int
Stroka
J
J
Neugebauer
M
M
D016604:Aflatoxin B1 / Q000032:analysis* / Q000633:toxicity; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D029786:Cyperus / Q000737:chemistry*; D004034:Diet Surveys; D005506:Food Contamination / Q000032:analysis*; D011042:Poisons / Q000032:analysis* / Q000633:toxicity; D018570:Risk Assessment / Q000379:methods; D013030:Spain
D011042:Poisons; D016604:Aflatoxin B1