Polyinosinic-polycytidylic acid induces the expression of GRO-alpha in BEAS-2B cells.
Growth-related oncogene protein-alpha (GRO-alpha)/CXCLl is a chemokine that activates neutrophils and plays an important role in inflammatory reactions. Polyinosinic-polycytidylic acid (poly IC) is a synthetic double-stranded RNA (dsRNA), which is a ligand for Toll-like receptor-3. Poly IC mimics viral infection when applied to cells and induces inflammatory and immune responses. In the present study, we found the induction of GRO-alpha in BEAS-2B bronchial epithelial cells treated with poly IC. Pretreatment of cells with 2-aminopurine, an inhibitor for dsRNA-dependent protein kinase (PKR), inhibited the expression of GRO-alpha-induced by poly IC. Overexpression of interferon-regulatory factor-3 (IRF-3) or retinoic-acid inducible gene-I (RIG-I) enhanced the induction of GRO-alpha by poly IC. PKR, IRF-3, and RIG-I may be involved in the poly IC-induced expression of GRO-alpha in BEAS-2B cells. Airway viral infection may elicit GRO-alpha expression in the bronchial epithelium, which may be implicated in inflammatory and immune reactions.
Yamashita
Koji
K
Department of Vascular Biology, Institute of Brain Science, University School of Medicine, Hirosaki, Japan.
Imaizumi
Tadaatsu
T
Taima
Kageaki
K
Fujita
Takashi
T
Ishikawa
Akira
A
Yoshida
Hidemi
H
Oyama
Chikara
C
Satoh
Kei
K
D001980:Bronchi / Q000166:cytology*; D002460:Cell Line; D054360:Chemokine CXCL1; D019743:Chemokines, CXC / Q000032:analysis / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism*; D007369:Interferon Inducers / Q000494:pharmacology*; D011070:Poly I-C / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D017077:Culture Media, Conditioned; D036341:Intercellular Signaling Peptides and Proteins; D007369:Interferon Inducers; D012333:RNA, Messenger; D011070:Poly I-C