title: Aqueous two-phase systems extraction of alpha-toxin from Clostridium perfringens type A.
abstract: Two sequential half-fraction designs were applied to studying the alpha-toxin partition produced by Clostridium perfringens type A in aqueous two phase systems (ATPS), as a function of four factors: PEG molar mass and concentration, phosphate concentration and pH. The highest purification factor, yield and partition coefficient results were obtained with PEG 8000 (15%, w/w), phosphate at 20% (w/w) and pH 8.0. This system allows, in a single step, an alpha-toxin purification of 4.6-fold with final activity yield of 230% and partition coefficient of 113.9 in the PEG rich phase.
authors.affiliation: Departament of Biochemical and Pharmaceutical Technology, University of São Paulo, Av. Prof. Lineu Prestes, 580, B.16. 05508-000, São Paulo, SP, Brazil.
authors.forename: Maria Taciana Holanda
authors.initials: MT
authors.lastname: Cavalcanti
authors.forename: Tatiana Souza
authors.initials: TS
authors.lastname: Porto
authors.forename: Benicio
authors.initials: B
authors.lastname: de Barros Neto
authors.forename: José Luiz
authors.initials: JL
authors.lastname: Lima-Filho
authors.forename: Ana Lúcia Figueiredo
authors.initials: AL
authors.lastname: Porto
authors.forename: Adalberto
authors.initials: A
authors.lastname: Pessoa
mesh_terms: D001427:Bacterial Toxins / Q000302:isolation & purification*; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D010738:Type C Phospholipases / Q000302:isolation & purification* / Q000378:metabolism; D014867:Water
chemical_list: D001427:Bacterial Toxins; D002135:Calcium-Binding Proteins; D014867:Water; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens