title: Cloning, expression, crystallization and preliminary X-ray characterization of cytochrome c552 from a moderate thermophilic bacterium, Hydrogenophilus thermoluteolus.
abstract: The amino-acid sequence of cytochrome c552 (PH c552) from a moderately thermophilic bacterium, Hydrogenophilus thermoluteolus, was more than 50% identical to that of cytochrome c from an extreme thermophile, Hydrogenobacter thermophilus (HT c552), and from a mesophile, Pseudomonas aeruginosa (PA c551). The PH c552 gene was overexpressed as a correctly processed holoprotein in the Escherichia coli periplasm. The overexpressed PH c552 has been crystallized by vapour diffusion from polyethylene glycol 4000 pH 6.5. The crystals belong to space group C222(1), with unit-cell parameters a = 48.98, b = 57.99, c = 56.20 A. The crystals diffract X-rays to around 2.1 A resolution.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Ichiki
authors.forename: Shota
authors.initials: S
authors.lastname: Nakamura
authors.forename: Tadayasu
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Jun
authors.initials: J
authors.lastname: Hasegawa
authors.forename: Susumu
authors.initials: S
authors.lastname: Uchiyama
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nishihara
authors.forename: Keiko
authors.initials: K
authors.lastname: Mizuta
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sambongi
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods; D003574:Cytochrome c Group / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000737:chemistry; D014835:Volatilization; D014961:X-Ray Diffraction
chemical_list: D003574:Cytochrome c Group; C022302:cytochrome C-552