[Promotion of wound healing through incorporation of bFGF-impregnated microspheres into collagen membrane].
OBJECTIVE
To investigate the promotion effects of the collagen membrane incorporating bFGF-impregnated microspheres on the wound healing of the pig skin losing its full-thickness layers.


METHODS
The bFGF containing microspheres was added into the dry microspheres. The collagen membranes were prepared by incorporating bFGF-impregnated microspheres, and 6 York pig models of skin wounds with loss of their full-thickness layers were established for the observation of the effects on the wound healing.


RESULTS
The healing time and the 28-day healing rate were 27.30 +/- 1.14 days and 98. 12% +/- 1.97%, respectively. The healing rate was significantly higher and the healing time was significantly shorter in the experimental group than in the control group (P < 0.05). The histological examination showed that the proliferation condition of the epidermis was also much better in the experimental group.


CONCLUSIONS
Incorporation of bFGF-impregnated microspheres into the collagen membrane is a promising method of promoting the healing of the wound with a loss of the full-thickness skin.
Huang
Sha
S
Tissue Engineering Center, Stomatological College, Fourth Military Medical University, Xi'an, Shaanxi 710032, P R China. huangsha@fmmu.edu.cn
Jin
Yan
Y
Deng
Tianzheng
T
Wu
Hong
H
Liu
Tao
T
Liu
Jianming
J
D000818:Animals; D003094:Collagen; D003692:Delayed-Action Preparations; D004195:Disease Models, Animal; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000494:pharmacology*; D008863:Microspheres; D012867:Skin / Q000293:injuries; D013552:Swine; D014945:Wound Healing / Q000187:drug effects*
D003692:Delayed-Action Preparations; D016222:Fibroblast Growth Factor 2; D003094:Collagen