In vitro evaluation of the antibacterial activity of Arctium lappa as a phytotherapeutic agent used in intracanal dressings.
The discovery of natural biocomponents from plants with antibacterial activity on endodontic microbiota may lead to new therapies. This study evaluated the antibacterial activity of a phytotherapeutic agent prepared from an ethyl acetate fraction (AcOEt) extracted from Arctium lappa. This agent was compared with calcium hydroxide as an intracanal dressing. Twenty-seven maxillary canines were instrumented, sterilized and inoculated with a mixed bacterial suspension of Pseudomonas aeruginosa, Escherichia coli, Lactobacillus acidophilus, Streptococcus mutans and Candida albicans. The teeth were divided into three groups and their canals filled with: group 1, calcium hydroxide and propylene glycol; group 2, a paste containing AcOEt fraction of A. lappa and propylene glycol; group 3, propylene glycol (control). At 7, 14 and 30 days, three teeth from each group were opened and a paper point was placed in the root canal for 5 min. The paper points were transferred to Petri dishes with Brain Heart Infusion (BHI). The bacterial growth was classified. Mild bacterial growth was found in group 1 at all time intervals; in group 2 there was severe growth at 7 days, but no growth at 14 and 30 days. The phytotherapeutic agent extracted from an AcOEt fraction of A. lappa inhibited the growth of all the microorganisms in this study.
Gentil
Marcelo
M
University of Ribeirão Preto, Av Costábile Romano, 2201, Ribeirânia, Ribeirão Preto, SP, Brazil, 14096-380.
Pereira
Juliana Vianna
JV
Sousa
Yara T Corrêa Silva
YT
Pietro
Rosimeire
R
Neto
Manoel D Sousa
MD
Vansan
Luiz Pascoal
LP
de Castro França
Suzelei
S
D000085:Acetates / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D031170:Arctium / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D001458:Bandages / Q000382:microbiology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D002177:Candidiasis / Q000188:drug therapy; D003786:Dental Pulp Cavity / Q000187:drug effects / Q000382:microbiology*; D006801:Humans; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018915:Root Canal Preparation / Q000379:methods; D013997:Time Factors
D000085:Acetates; D000900:Anti-Bacterial Agents; D010936:Plant Extracts; C007650:ethyl acetate; D002126:Calcium Hydroxide