Gene targeting in plants: fingers on the move.
Zinc-finger endonucleases (ZFNs) make targeted double-stranded breaks in genomic DNA and, thus, stimulate recombination and repair processes at specific sites. ZFNs can now be harnessed to stimulate homologous recombination and gene targeting in plants, which represents a major step towards modifying the plant genome more precisely. ZFN-mediated gene targeting is likely to become a powerful tool for genome research and genetic engineering.
Kumar
Sandeep
S
Department of Botany, North Carolina State University, Raleigh, NC 27695-7612, USA. skumar3@unity.ncsu.edu
Allen
George C
GC
Thompson
William F
WF
D042002:Chromatin Assembly and Disassembly; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000737:chemistry* / Q000378:metabolism*; D018390:Gene Targeting / Q000379:methods*; D018745:Genome, Plant; D010944:Plants / Q000235:genetics*; D011995:Recombination, Genetic; D016335:Zinc Fingers / Q000502:physiology*
D004706:Endodeoxyribonucleases