Pharmacokinetic study of p-coumaric acid in mouse after oral administration of extract of Ananas comosus L. leaves.
Quantification of p-coumaric acid in mouse plasma following oral administration of Ananas comosus L. leaves was achieved by reversed-phase high-performance liquid chromatography using a mobile phase of water-acetonitrile (82:18, v/v) and UV detection at 310 nm. The method was linear (determination coefficient, r2 = 0.9997) within the tested range (0.04-1.28 microg/mL). Intra- and inter-day precision coefficients of variation and accuracy bias were acceptable (maximal CV value was 4.06% for intra-day and 4.19% for inter-day) over the entire range. The recoveries were 90.63, 97.98 and 100.01% for concentrations of 0.04, 0.32 and 1.28 microg/mL, respectively. This is a very rapid, sensitive and economical way to determine p-coumaric acid concentration in mouse plasma after oral administration of A. comosus leaves. The concentration-time curve was fitted to the one-compartment model. This is the first time that p-coumaric acid extracted from A. comosus leaves was detected by HPLC-UV method and its pharmacokinetic characteristic was comprehensively studied.
Meng
Zhen
Z
Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
Wang
Wei
W
Xing
Dong-Ming
DM
Lei
Fan
F
Lan
Jia-Qi
JQ
Du
Li-Jun
LJ
D000284:Administration, Oral; D031230:Ananas / Q000737:chemistry*; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry*; D011422:Propionates; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D003373:Coumaric Acids; D010936:Plant Extracts; D011422:Propionates; C495469:p-coumaric acid