Spatial fluorescence cross-correlation spectroscopy.
We present an alternative method for diffusion measurements of fluorescent species in solution by use of confocal microscopy and fluorescence correlation spectroscopy techniques. It consists of making a time and spatial dual correlation in which one detects the fluorescence signals from two nearby separate confocal volumes and cross correlates them. To improve the spatial discrimination between the two confocal volumes we propose filtering of fluorescence photocounts by rejecting the fluorescence background, which corresponds to particles located far from the center of the detection volumes.
Jaffiol
Rodolphe
R
Laboratoire de Spectrometrie Physique, UMR CNRS 5588, Universite Joseph Fourier, B.P. 87, 38402 Saint Martin d'Heres cedex, France. rodolphe.jaffiol@utt.fr
Blancquaert
Yoann
Y
Delon
Antoine
A
Derouard
Jacques
J
D000465:Algorithms; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013223:Statistics as Topic