title: Degradation of a Plasticizer, di-n-Butylphthalate by Delftia sp. TBKNP-05.
abstract: A bacterial strain Delftia sp. TBKNP-05 isolated by para-hydroxybenzoate enrichment technique is capable of degrading di-n-butylphthalate (DBP) as a sole source of carbon and energy. Analysis of intermediates by thin layer chromatography and high performance liquid chromatography indicated the presence of monobutylphthalate (MBP), phthalate (PA), and protocatechuate (PCA). The washed cells grown on DBP and PA showed appreciable oxidation of DBP, MBP, PA, and PCA. The enzyme activities in cell free extracts of Delftia sp. TBKNP-05 exhibited the presence of DBP esterase, MBP esterase, PA-dioxygenase, and protocatechuate 4, 5-dioxygenase. The protocatechuate is metabolized by a meta-cleavage pathway leading to further mineralization of the compound in this bacterium.
authors.affiliation: Department of Biochemistry, Gulbarga University, Gulbarga-585 106, Karnataka, India. goudartbk@rediffmail.com
authors.forename: Neelakanteshwar K
authors.initials: NK
authors.lastname: Patil
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kundapur
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
authors.forename: T B
authors.initials: TB
authors.lastname: Karegoudar
mesh_terms: D001673:Biodegradation, Environmental; D001692:Biological Transport; D042742:Delftia / Q000145:classification / Q000201:enzymology / Q000378:metabolism*; D003993:Dibutyl Phthalate / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000032:analysis; D010100:Oxygen / Q000378:metabolism; D010795:Phthalic Acids / Q000032:analysis; D010802:Phylogeny; D010968:Plasticizers / Q000378:metabolism*
chemical_list: D062385:Hydroxybenzoates; D010795:Phthalic Acids; D010968:Plasticizers; D003993:Dibutyl Phthalate; C009091:protocatechuic acid; C032279:phthalic acid; D010100:Oxygen; C028577:monobutyl phthalate