The 47kDa Akt substrate associates with phosphodiesterase 3B and regulates its level in adipocytes.
We have previously described a novel putative 47kDa substrate for the protein kinase Akt (designated AS47) in 3T3-L1 adipocytes. In the present study, we have found by co-immunoprecipitation that AS47 was associated with cyclic nucleotide phosphodiesterase 3B (PDE3B) in lysates of 3T3-L1 adipocytes. The patterns of expression of AS47 and PDE3B upon 3T3-L1 adipocyte differentiation, among mouse tissues, and in adipocytes with and without the transcription factor C/EBPalpha were virtually coincident. Partial knockdown of AS47 in 3T3-L1 adipocytes with shRNA resulted in a similar reduction in PDE3B protein. These results indicate that AS47 exists in a complex with PDE3B in adipocytes and that the amount of AS47 protein regulates the amount of PDE3B.
Chavez
Jose A
JA
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA.
Gridley
Scott
S
Sano
Hiroyuki
H
Lane
William S
WS
Lienhard
Gustav E
GE
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D041721:3T3-L1 Cells; D017667:Adipocytes; D000818:Animals; D002265:Carboxylic Ester Hydrolases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D005786:Gene Expression Regulation / Q000502:physiology; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D013379:Substrate Specificity
D008565:Membrane Proteins; D010750:Phosphoproteins; D051057:Proto-Oncogene Proteins c-akt; C510002:Abhd15 protein, mouse; D002265:Carboxylic Ester Hydrolases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; C517293:Pde3b protein, mouse