title: Mitogen-activated protein kinase/extracellular signal-regulated protein kinase activation of cultured human dental pulp cells by low-power gallium-aluminium-arsenic laser irradiation.
abstract: OBJECTIVE
  To examine whether low-power laser irradiation (LPLI) promotes cellular proliferation of human dental pulp-derived fibroblast-like cells (dental pulp cells).
  
  
  METHODS
  Dental pulp cells were obtained by primary culture of human dental pulp tissues from extracted third molar teeth. The phosphorylation of the mitogen-activated protein kinase (MAPK) family after LPLI of these cells was investigated by Western blotting. By using a specific MAPK/ERK kinase (MEK) inhibitor (PD098059), the specific effect of LPLI on the MAPK pathway was also investigated by Western blotting as described above. The incorporation of [3H]thymidine into the cells after LPLI was determined, and statistical analysis was performed by Wilcoxon signed-ranks test.
  
  
  RESULTS
  Extracellular signal-regulated protein kinase (ERK) 1/2 was phosphorylated between 5 and 30 min after LPLI. Moreover, PD098059 inhibited LPLI-mediated ERK1/2 activation. LPLI did not affect p38 MAPK or c-Jun N-terminal kinase (JNK) phosphorylation. But LPLI did not stimulate [3H]thymidine incorporation into these cells.
  
  
  CONCLUSIONS
  These results indicated that LPLI activated MAPK/ERK, a signal for proliferation, differentiation and survival, but did not activate the stress signals p38 MAPK and JNK in human dental pulp cells.
authors.affiliation: Department of Endodontics, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Miyata
authors.forename: T
authors.initials: T
authors.lastname: Genma
authors.forename: M
authors.initials: M
authors.lastname: Ohshima
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: O
authors.initials: O
authors.lastname: Takeichi
authors.forename: B
authors.initials: B
authors.lastname: Ogiso
authors.forename: K
authors.initials: K
authors.lastname: Otsuka
mesh_terms: D003782:Dental Pulp / Q000166:cytology / Q000528:radiation effects*; D004789:Enzyme Activation / Q000528:radiation effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism / Q000528:radiation effects*; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D053685:Laser Therapy; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism / Q000528:radiation effects*; D018709:Statistics, Nonparametric
chemical_list: D048049:Extracellular Signal-Regulated MAP Kinases; D020928:Mitogen-Activated Protein Kinases