Association of urokinase gene 3'-UTR polymorphism with calcium oxalate nephrolithiasis.
OBJECTIVE
Urokinase might play a role in the formation of kidney stones. The aim of this study was to investigate the role of the urokinase gene in calcium oxalate nephrolithiasis.


METHODS
A control group of 150 healthy individuals having no history of stone formation (mean age 40 +/- 11.5 years) and a group of 130 patients (mean age 40.5 +/- 10.5 years) with recurrent calcium oxalate stones were examined. The C/T polymorphism of the urokinase gene was detected using polymerase chain reaction (PCR)-based restriction analysis.


RESULTS
A marginally significant difference (P = 0.035) was found in the distribution of the urokinase gene 3' untranslated region (UTR) C/T polymorphism between patients with stones and controls. The odds ratio for the risk of the T allele in stone patients was 1.006 (95% CI 0.63-1.62).


CONCLUSIONS
The T allele of 3' UTR of the urokinase gene may not be associated with a higher risk of stone formation.
Mittal
Rama D
RD
Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India. rmittl@sgpgi.ac.in
Bid
Hemant K
HK
Kumar
Anant
A
Bhandari
M
M
D000328:Adult; D000483:Alleles; D002129:Calcium Oxalate / Q000652:urine*; D004247:DNA / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007669:Kidney Calculi / Q000201:enzymology* / Q000235:genetics / Q000652:urine; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011379:Prognosis; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*
D002129:Calcium Oxalate; D004247:DNA; D014568:Urokinase-Type Plasminogen Activator