title: [Neuroprotection of chloride channel blockers against NMDA-induced apoptosis of cultured rat hippocampal neurons].
abstract: Activation of N-methyl-d-aspartic acid (NMDA) receptor plays an important role in neuronal apoptosis induced by cerebral ischemia but the underlying mechanisms are still unclear. The present study examined the neuroprotection of three chloride blockers in an in vitro cell model of cerebral ischemia established by treatment of cultured rat hippocampal neurons with NMDA. Hoechst 33258 staining and MTT assay were used to detect neuronal apoptosis and cell viability, respectively. The neuroprotective effects of chloride channel blockers on the cell viability and neuronal apoptosis were only observed when the blockers were applied before NMDA exposure. In comparison with DIDS, SITS showed more potent protective effect in a dose-dependent manner, whereas NPPB showed no significant neuroprotective effect. The results demonstrate that pretreatment with both SITS and DIDS have protective effect against neuronal apoptosis, which is achieved by blocking both NMDA receptor and chloride channel.
authors.affiliation: Department of Neurobiology, Southern Medical University, Guangzhou 510515, China. qzchang@tom.com
authors.forename: Quan-zhong
authors.initials: QZ
authors.lastname: Chang
authors.forename: De-hui
authors.initials: DH
authors.lastname: Hu
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Tian-ming
authors.initials: TM
authors.lastname: Gao
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D006690:Bisbenzimidazole / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018118:Chloride Channels / Q000037:antagonists & inhibitors*; D006624:Hippocampus / Q000166:cytology; D008856:Microscopy, Fluorescence; D016202:N-Methylaspartate / Q000494:pharmacology*; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018118:Chloride Channels; D018696:Neuroprotective Agents; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D016202:N-Methylaspartate; D006690:Bisbenzimidazole; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid