Comparison of the compact dry EC with the most probable number method (AOAC official method 966.24) for enumeration of Escherichia coli and coliform bacteria in raw meats. Performance-Tested Method 110402.
Compact Dry E. coli/Coliform Count (EC) is a ready-to-use test method for the enumeration of Escherichia coli and coliform bacteria in food. The plates are presterilized and contain culture medium and a cold water-soluble gelling agent. The medium should be rehydrated with 1 mL diluted sample inoculated onto the center of the self-diffusible medium, allowing the solution to diffuse by capillary action. The plate can be incubated at 35 degrees C for 20-24 h and the colonies counted without any further working steps. The Compact Dry EC medium plates were validated as an analysis tool for determining colony-forming units (CFU) of E. coli and coliform bacteria from a variety of raw meats using 5 different types of raw meats. The performance tests were conducted at 35 degrees C. In all studies performed, no apparent differences were observed between the Compact Dry EC method and the AOAC Official Method 966.24 results. For the accuracy claim (n = 75), a correlation factor of r2 = 0.93 (E. coli) and r2 = 0.93 (coliform bacteria) could be assigned, as stated in the application for "Performance-Tested Method."
Kodaka
Hidemasa
H
Nissui Pharmaceutical Co. Ltd., 1075-2, Hokunanmoro, Yuki, Ibaraki 307-0036, Japan. h-kodaka@yki.nissui-pharm.jp
Mizuochi
Shingo
S
Teramura
Hajime
H
Nirazuka
Tadanobu
T
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000378:metabolism*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D008461:Meat Products / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012756:Sheep; D013552:Swine; D013696:Temperature; D013997:Time Factors