A colorimetric assay for S-adenosylhomocysteine hydrolase.
A colorimetric method for S-adenosyl-L-homocysteine hydrolase (SAHase) which uses S-adenosyl-L-homocysteine (SAH) as substrate is described. This method involves the hydrolytic conversion of SAH into adenosine (ADO) and L-homocysteine (HCY). The formation of HCY is quantified using Ellman's reagent and spectrophotometrical measured at 412 nm. Under these assay conditions, the product was followed continuously in a facile and quantitative manner until substrate conversion was complete. This method is an easy, cheap and shorter alternative to more complex methods and it is applicable to routine clinical analysis and in the assay and development of new S-nucleosidylhomocysteines to be used as therapeutic compounds.
Lozada-Ramírez
J D
JD
Department of Biochemistry and Molecular Biology-A, Faculty of Biology, University of Murcia, Campus Espinardo, E-30071 MURCIA, Spain.
Martínez-Martínez
I
I
Sánchez-Ferrer
A
A
García-Carmona
F
F
D000243:Adenosine Deaminase / Q000494:pharmacology; D043383:Adenosylhomocysteinase / Q000493:pharmacokinetics*; D003124:Colorimetry / Q000379:methods*; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D008961:Models, Structural
D004228:Dithionitrobenzoic Acid; D043383:Adenosylhomocysteinase; D000243:Adenosine Deaminase