title: Vesicular glutamate transporter 3 (VGLUT3) identifies spatially segregated excitatory terminals in the rat substantia nigra.
abstract: The excitability of dopaminergic (DA) neurons in the substantia nigra is controlled by the convergent activity of multiple glutamatergic afferents. Here, we show that vesicular glutamate transporter 3 (VGLUT3)-immunoreactive (ir) terminals segregate to the perisomatic region of DA neurons in the substantia nigra pars compacta, and VGLUT3 decorates a synapse population distinct from those marked by vesicular glutamate transporters 1 and 2. VGLUT3-ir nerve endings form asymmetric terminals on DA neurons. Retrograde tracing suggests the superior colliculus as an origin of excitatory VGLUT3-ir afferents. Collectively, our data indicate that VGLUT3 identifies a novel excitatory terminal subset that contributes to the tuning of DA cell excitability in the substantia nigra.
authors.affiliation: Laboratory for Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Scheeles väg 1:A1.
authors.forename: Raquel
authors.initials: R
authors.lastname: Martín-Ibañez
authors.forename: Monica
authors.initials: M
authors.lastname: Jenstad
authors.forename: Paul
authors.initials: P
authors.lastname: Berghuis
authors.forename: Robert H
authors.initials: RH
authors.lastname: Edwards
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hioki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kaneko
authors.forename: Jan
authors.initials: J
authors.lastname: Mulder
authors.forename: Josep M
authors.initials: JM
authors.lastname: Canals
authors.forename: Patrik
authors.initials: P
authors.lastname: Ernfors
authors.forename: Farrukh A
authors.initials: FA
authors.lastname: Chaudhry
authors.forename: Tibor
authors.initials: T
authors.lastname: Harkany
mesh_terms: D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D002867:Chromones; D003911:Dextrans / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods; D016253:Microscopy, Immunoelectron / Q000379:methods; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013378:Substantia Nigra / Q000166:cytology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D050988:Vesicle-Associated Membrane Protein 2 / Q000378:metabolism; D050599:Vesicular Glutamate Transport Protein 2 / Q000378:metabolism; D050496:Vesicular Glutamate Transport Proteins / Q000378:metabolism*
chemical_list: C470766:5-hydroxy-2-(14'-nonadecenyl) chromone; D002867:Chromones; D003911:Dextrans; D043884:Receptor, Cannabinoid, CB1; C482103:Slc17a8 protein, rat; D050988:Vesicle-Associated Membrane Protein 2; D050599:Vesicular Glutamate Transport Protein 2; D050496:Vesicular Glutamate Transport Proteins; C076397:biotinylated dextran amine; D012701:Serotonin; D001710:Biotin; D014446:Tyrosine 3-Monooxygenase; D010751:Phosphopyruvate Hydratase