Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds.
A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 A resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P2(1)2(1)2(1). Crystallographic refinement and full amino-acid sequence determination are in progress.
Cavada
Benildo S
BS
Department of Biochemistry, Federal University of Ceará, Brazil. bscavada@ufc.br
Marinho
Emmanuel S
ES
Souza
Emmanuel P
EP
Benevides
Raquel G
RG
Delatorre
Plínio
P
Souza
Luis A G
LA
Nascimento
Kyria S
KS
Sampaio
Alexandre H
AH
Moreno
Frederico B M B
FB
Rustiguel
Joane K R
JK
Canduri
Fernanda
F
de Azevedo
Walter F
WF
Debray
Henri
H
D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D007887:Fabaceae / Q000737:chemistry*; D006384:Hemagglutination; D008358:Mannose / Q000737:chemistry; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits; D012639:Seeds / Q000737:chemistry*
D037121:Plant Lectins; D008358:Mannose