Determination of nucleic acids based on the quenching effect on resonance light scattering of the Y(III)-1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolone-4'-)hexane-dione system.
Nucleic acids can quench resonance light scattering (RLS) intensity of the Y(III)-1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolone-4'-)hexane-dione(BPMPHD) complex in the pH range 5.0-5.8. Under optimal conditions, there are linear relationships between the quenching of RLS and the concentration of nucleic acids in the range 6.3 x 10(-8)-2.1 x 10(-5) g/mL for fish sperm DNA (fsDNA), 1.2 x 10(-8)-5.0 x 10(-5) g/mL for calf thymus DNA (ctDNA) and 6.0 x 10(-8)-2.0 x 10(-5) g/mL for yeast RNA (yRNA). The detection limits (3 s) of fsDNA, ctDNA and yRNA are 0.7 ng/mL, 3.8 ng/mL and 4.2 ng/mL, respectively.
Wu
Xia
X
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
Yang
Jing He
JH
Sun
Shuna
S
Guo
Changying
C
Ran
Dehuan
D
Zheng
Jinhua
J
D000447:Aldehydes / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000737:chemistry; D005399:Fishes; D006863:Hydrogen-Ion Concentration; D008297:Male; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry*; D011720:Pyrazoles / Q000737:chemistry*; D012313:RNA / Q000032:analysis / Q000737:chemistry; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013094:Spermatozoa / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism
C512861:1,6-bi(1'-phenyl-3'-methyl-5'-pyrazolon-4'-)hexanedione; D000447:Aldehydes; D009696:Nucleic Acids; D011720:Pyrazoles; D012313:RNA; D004247:DNA; C427203:calf thymus DNA