title: Crystallization and preliminary X-ray crystallographic studies of Mycobacterium tuberculosis chorismate mutase.
abstract: Chorismate mutase catalyzes the first committed step in the biosynthesis of the aromatic amino acids phenylalanine and tyrosine in bacteria, fungi and higher plants. The recent re-annotation of the Mycobacterium tuberculosis genome has revealed the presence of a duplicate set of genes coding for chorismate mutase. The mycobacterial gene Rv1885c bears <20% sequence homology to other bacterial chorismate mutases, thus serving as a potential target for the development of inhibitors specific to the pathogen. The M. tuberculosis chorismate mutase was crystallized in space group C2 and the crystals diffracted to a resolution of 2.2 A. Matthews coefficient and self-rotation function calculations revealed the presence of two monomers in the asymmetric unit.
authors.affiliation: Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam, Hyderabad 500076, India.
authors.forename: Rohini
authors.initials: R
authors.lastname: Qamra
authors.forename: Prachee
authors.initials: P
authors.lastname: Prakash
authors.forename: Bandi
authors.initials: B
authors.lastname: Aruna
authors.forename: Seyed E
authors.initials: SE
authors.lastname: Hasnain
authors.forename: Shekhar C
authors.initials: SC
authors.lastname: Mande
mesh_terms: D002826:Chorismate Mutase / Q000737:chemistry* / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D019897:Periplasm / Q000201:enzymology; D011302:Prephenate Dehydratase / Q000378:metabolism; D011303:Prephenate Dehydrogenase / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D011303:Prephenate Dehydrogenase; D011302:Prephenate Dehydratase; D002826:Chorismate Mutase