title: Characterization of Bovine herpesvirus type 4 isolated from cattle with mastitis and subclinical infection by the virus among cattle.
abstract: An outbreak of contagious mastitis occurred among cattle on a farm, and bovine herpesviruses were isolated from the affected mammary tissues, scabs and abscess discharge of the cattle. A bovine herpesvirus type 4 (BoHV-4)-specific fragment was amplified from the isolates by polymerase chain reaction (PCR). Restriction endonuclease analyses demonstrated that the isolates were related to Movar-like European type BoHV-4. To determine the ratio of BoHV-4 subclinical infection in the cattle, a genomic survey was performed by PCR for cattle that were moved to the animal hygiene service station in Ibaraki prefecture. The BoHV-4 genome was occasionally detected in peripheral blood leukocytes, lymph nodes and nervous tissues. The rate of BoHV-4 subclinical infection was relatively high in the cattle.
authors.affiliation: National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Izumi
authors.forename: Satoko
authors.initials: S
authors.lastname: Tsuduku
authors.forename: Kenji
authors.initials: K
authors.lastname: Murakami
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Tsuboi
authors.forename: Misako
authors.initials: M
authors.lastname: Konishi
authors.forename: Makoto
authors.initials: M
authors.lastname: Haritani
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kamiyoshi
authors.forename: Kumiko
authors.initials: K
authors.lastname: Kimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sentsui
mesh_terms: D000038:Abscess / Q000662:veterinary / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D026021:Herpesvirus 4, Bovine / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D014412:Tumor Virus Infections / Q000662:veterinary* / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral