title: IL-18 gene polymorphisms affect IL-18 production capability by monocytes.
abstract: We previously demonstrated a significant association between IL-18 gene polymorphism 105A/C and asthma. In this study, we investigated the relationship of IL-18 gene polymorphism to IL-18 production capability by monocytes. The frequency of gene polymorphisms including IL-18-105A/C and IL-18--137G/C was determined by PCR analyses. The IL-18 production by monocytes stimulated without or with LPS or A23187+PMA for 1day was measured by ELISA. The produced IL-18 spontaneously or in response to A23187+PMA by monocytes was significantly higher for volunteers with 105A/A genotype than with 105A/C genotype. Similarly, the production capability of IL-18 by monocytes from volunteers with -137G/G genotype was significantly higher than that with -137G/C genotype and significant linkage disequilibrium was observed between 105A/C and -137G/C polymorphism. Thus, the genetic capacity to produce more IL-18 in response to stimuli may affect the onset of asthma.
authors.affiliation: Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Medical School, Osaka 565-0871, Japan.
authors.forename: Junsuke
authors.initials: J
authors.lastname: Arimitsu
authors.forename: Toru
authors.initials: T
authors.lastname: Hirano
authors.forename: Shinji
authors.initials: S
authors.lastname: Higa
authors.forename: Mari
authors.initials: M
authors.lastname: Kawai
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Naka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ogata
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Shima
authors.forename: Minoru
authors.initials: M
authors.lastname: Fujimoto
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yamadori
authors.forename: Keisuke
authors.initials: K
authors.lastname: Hagiwara
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Ohgawara
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kuwabara
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kawase
authors.forename: Toshio
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D001249:Asthma / Q000235:genetics* / Q000276:immunology*; D005260:Female; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics* / Q000276:immunology*; D015810:Linkage Disequilibrium / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology*; D011110:Polymorphism, Genetic / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D020382:Interleukin-18