Quasielastic light scattering study of amyloid beta-protein fibril formation.
Quasielastic light scattering spectroscopy (QLS) is an optical method for the determination of diffusion coefficients of particles in solution. Here we discuss the principles of QLS and explain how the distribution of particle sizes can be reconstructed from the measured correlation function of scattered light. Non-invasive observation of the temporal evolution of particle sizes provides a powerful tool for studying protein assembly. We illustrate practical applications of QLS with examples from studies of fibril formation of the amyloid beta-protein.
Lomakin
Aleksey
A
Department of Physics and Center for Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. aleksey@mit.edu
Teplow
David B
DB
D016229:Amyloid beta-Peptides / Q000096:biosynthesis* / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D008027:Light; D012542:Scattering, Radiation
D016229:Amyloid beta-Peptides