The structure at 2.4 A resolution of the protein from gene locus At3g21360, a putative Fe(II)/2-oxoglutarate-dependent enzyme from Arabidopsis thaliana.
The crystal structure of the gene product of At3g21360 from Arabidopsis thaliana was determined by the single-wavelength anomalous dispersion method and refined to an R factor of 19.3% (Rfree = 24.1%) at 2.4 A resolution. The crystal structure includes two monomers in the asymmetric unit that differ in the conformation of a flexible domain that spans residues 178-230. The crystal structure confirmed that At3g21360 encodes a protein belonging to the clavaminate synthase-like superfamily of iron(II) and 2-oxoglutarate-dependent enzymes. The metal-binding site was defined and is similar to the iron(II) binding sites found in other members of the superfamily.
Bitto
Eduard
E
Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin-Madison, USA.
Bingman
Craig A
CA
Allard
Simon T M
ST
Wesenberg
Gary E
GE
Aceti
David J
DJ
Wrobel
Russell L
RL
Frederick
Ronnie O
RO
Sreenath
Hassan
H
Vojtik
Frank C
FC
Jeon
Won Bae
WB
Newman
Craig S
CS
Primm
John
J
Sussman
Michael R
MR
Fox
Brian G
BG
Markley
John L
JL
Phillips
George N
GN
D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D003460:Crystallization; D018360:Crystallography, X-Ray; D054637:Dual-Specificity Phosphatases; D007501:Iron / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry* / Q000378:metabolism
D029681:Arabidopsis Proteins; D007656:Ketoglutaric Acids; D007501:Iron; D006899:Mixed Function Oxygenases; C066046:clavaminate synthase; C481271:DsPTP1 protein, Arabidopsis; D054637:Dual-Specificity Phosphatases; D017027:Protein Tyrosine Phosphatases