Protective effects of Korean herbal remedy against oxidative stress in cardiomyocytes.
Ondamtanggagambang (ODG) has been used as a prescription for psychological anxiety and depression in Korean medicine. In this study, we found that ODG have protective effects against oxidative stress in cardiomyocytes. Pretreatment with ODG extract prevented H2O2 and ZnCl2-induced cell damage in H9c2 cardiomyocytes, whereas simultaneous treatment of ODG extract did not. The protective effect of ODG extract on oxidative stress-induced damage was suppressed significantly by heme oxygenase (HO) inhibitors, zinc protoporphyrin-IX (ZnPP-IX, p < 0.01) and tin protoporphyrin-IX (SnPP-IX, p < 0.01) in H9c2 cells. ODG stimulation of cells strongly induced the expression of HO-1 protein. Taken together, it is suggested that ODG-induced expression of HO-1 may have a beneficial role in cardiomyocytes under oxidative stress.
Kim
Myung-Sunny
MS
Korea Food Research Institute, Kyongki-do, 463-746, Republic of Korea.
Kwon
Dae Young
DY
Cho
Hye-Jin
HJ
Lee
Myeong Soo
MS
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D002712:Chlorides / Q000494:pharmacology; D029001:Herbal Medicine; D015151:Immunoblotting / Q000379:methods; D007723:Korea; D019684:Magnoliopsida / Q000737:chemistry*; D008665:Metalloporphyrins / Q000494:pharmacology; D000432:Methanol / Q000737:chemistry; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D011524:Protoporphyrins / Q000494:pharmacology; D051381:Rats; D017967:Zinc Compounds / Q000494:pharmacology
D000906:Antibodies; D002712:Chlorides; D008665:Metalloporphyrins; D010936:Plant Extracts; D011524:Protoporphyrins; D017967:Zinc Compounds; C017803:zinc protoporphyrin; C016837:zinc chloride; C032628:tin protoporphyrin IX; D000432:Methanol