title: Correlation of in vitro cytokine responses with the chemical composition of soil-derived particulate matter.
abstract: We treated human lung epithelial cells, type BEAS-2B, with 10-80 microg/cm2 of dust from soils and road surfaces in the western United States that contained particulate matter (PM) < 2.5 microm aerodynamic diameter. Cell viability and cytokine secretion responses were measured at 24 hr. Each dust sample is a complex mixture containing particles from different minerals mixed with biogenic and anthropogenic materials. We determined the particle chemical composition using methods based on the U.S. Environmental Protection Agency Speciation Trends Network (STN) and the National Park Service Interagency Monitoring of Protected Visual Environments (IMPROVE) network. The functionally defined carbon fractions reported by the ambient monitoring networks have not been widely used for toxicology studies. The soil-derived PM2.5 from different sites showed a wide range of potency for inducing the release of the proinflammatory cytokines interleukin-6 (IL-6) and IL-8 in vitro. Univariate regression and multivariate redundancy analysis were used to test for correlation of viability and cytokine release with the concentrations of 40 elements, 7 ions, and 8 carbon fractions. The particles showed positive correlation between IL-6 release and the elemental and pyrolyzable carbon fractions, and the strongest correlation involving crustal elements was between IL-6 release and the aluminum:silicon ratio. The observed correlations between low-volatility organic components of soil- and road-derived dusts and the cytokine release by BEAS-2B cells are relevant for investigation of mechanisms linking specific air pollution particle types with the initiating events leading to airway inflammation in sensitive populations.
authors.affiliation: Department of Pharmacology and Toxicology, University of Utah, Salt Lake City, Utah 84112-5820, USA. John.Veranth@utah.edu
authors.forename: John M
authors.initials: JM
authors.lastname: Veranth
authors.forename: Tyler A
authors.initials: TA
authors.lastname: Moss
authors.forename: Judith C
authors.initials: JC
authors.lastname: Chow
authors.forename: Raed
authors.initials: R
authors.lastname: Labban
authors.forename: William K
authors.initials: WK
authors.lastname: Nichols
authors.forename: John C
authors.initials: JC
authors.lastname: Walton
authors.forename: John G
authors.initials: JG
authors.lastname: Watson
authors.forename: Garold S
authors.initials: GS
authors.lastname: Yost
mesh_terms: D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity*; D002244:Carbon / Q000032:analysis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004391:Dust / Q000032:analysis; D004784:Environmental Monitoring; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D008345:Manganese / Q000032:analysis / Q000633:toxicity; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*
chemical_list: D000393:Air Pollutants; D004391:Dust; D015850:Interleukin-6; D016209:Interleukin-8; D012989:Soil Pollutants; D008345:Manganese; D002244:Carbon