title: [Kinetic turbidimetric limulus assay of bacterial endotoxin in the replacement solution of on-line hemodiafiltration].
abstract: OBJECTIVE
  To determine bacterial endotoxin in the replacement solution of on-line hemodiafiltration (on-line HDF) using kinetic turbidimetric limulus test.
  
  
  RESULTS
  Validation test was performed with the replacement solution of on-line HDF in which quantified standard endotoxin was added. The recovery rates of endotoxin from the replacement solution and its dilutions at 1/5, 1/10, and 1/20 were 58.17%, 106.7%, 99.00% and 98.79%, respectively, suggesting that the optimal dilution was at 1/10. Standard endotoxin was added into the replacement solution of on-line HDF of 3 batches (040408, 040511,040527), and the recovery rates in their dilution at 1/10 were 76.32%, 99.00% and 96.24%, respectively. The standard endotoxin in the working curve was 1.00, 0.125, and 0.0156 Eu/ml (endotoxin unit/ml), and the dilution at 1/10 of the replacement solution is effective to eliminate the interference in limulus test.
  
  
  CONCLUSIONS
  Kinetic turbidimetric limulus test provide a means to detect endotoxin in the replacement solution of on-line HDF.
authors.affiliation: Department of Pharmacy, People's Hospital of Guangdong Province, Guangzhou 510080, China. laohaiyan1208@vip.sina.com
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Lao
authors.forename: Qiu-xiao
authors.initials: QX
authors.lastname: Lin
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Xin-ling
authors.initials: XL
authors.lastname: Liang
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Ju-jin
authors.initials: JJ
authors.lastname: Feng
mesh_terms: D004731:Endotoxins / Q000032:analysis*; D017583:Hemodiafiltration / Q000379:methods*; D015312:Hemodialysis Solutions / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D008033:Limulus Test; D009391:Nephelometry and Turbidimetry / Q000379:methods
chemical_list: D004731:Endotoxins; D015312:Hemodialysis Solutions