Sequence analysis of the haemagglutinin and fusion protein genes of peste-des-petits ruminants vaccine virus of Indian origin.
The amino acid composition of the two surface proteins of peste-des-petits ruminants vaccine virus belonging to lineage four from India were deduced from the nucleotide sequence. The fusion (F) protein gene of PPRV Sungri/96 is 2405 nucleotides long and in relation to the length, it is 80 nucleotides longer than that of PPRV Nigeria/75/1 which are found to be present at the 5'UTR of this virus. The complete F gene alignment with other morbillivirus reveals a homology of 89% with PPRV/Nigeria/75/1 and 48-51% with other morbilliviruses. The F protein of PPRV Sungri/96 exhibited characteristics similarity to those of other morbillivirus F proteins. The overall amino acid similarity with its counterpart PPRV Nigeria/75/1 was 96%; with other morbilliviruses it is 65-74%. The PPRV Sungri/96 haemagglutinin (H) protein gene is 1954 nucleotides long and showed a sequence homology of 90.7% with PPRV/Nigeria/75/1 and with other morbilliviruses it ranged from 33% to 45%. At amino acids level, PPRV Sungri/96 showed a homology of 92.3% with PPRV/Nigeria/75/1 and 34-49% with other morbilliviruses. The phylogenetic tree constructed for F and H gene reveals four separate groups which is very similar to that found in other genes. To the best of our knowledge this is the first report describing the F and H genes of an Indian isolate.
Dhar
P
P
Indian Veterinary Research Institute, Izatanagar, 243 122, Bareilly, India.
Muthuchelvan
D
D
Sanyal
A
A
Kaul
R
R
Singh
R P
RP
Singh
R K
RK
Bandyopadhyay
S K
SK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics; D007194:India; D008969:Molecular Sequence Data; D018107:Peste-des-petits-ruminants virus / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014760:Viral Fusion Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D014760:Viral Fusion Proteins; D014765:Viral Vaccines