title: Complement C3c and related protein biomarkers in amyotrophic lateral sclerosis and Parkinson's disease.
abstract: We have used quantitative 2D gel electrophoresis to analyze serum proteins from 422 patients with neurodegenerative diseases and normal individuals in an unbiased approach to identify biomarkers. Differences in abnormal serum levels were found between amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD), and related disorders for 34 protein biomarker spots, nine of which were related to the complement system. Of these nine, four spots originated from the Complement C3b-alpha-chain (C3c(1), C3c(2a), C3c(2b), and C3dg). The C3c spots (C3c(1), C3c(2a), and C3c(2b)) had the same amino acid sequence and glycosylation, though only C3c(1) was phosphorylated. In addition, Complement Factors H, Bb, and Pre-Serum amyloid protein displayed different serum concentrations in ALS, PD, and normal sera, whereas Complement C4b gamma-chain and Complement Factor I did not. The differential expression of the complement proteins provides potentially useful biomarkers as well as evidence for the involvement of inflammatory processes in the pathogenesis of ALS and PD.
authors.affiliation: Power3 Medical Products, Inc., The Woodlands, TX, USA. igoldknopf@power3.medical.com
authors.forename: Ira L
authors.initials: IL
authors.lastname: Goldknopf
authors.forename: Essam A
authors.initials: EA
authors.lastname: Sheta
authors.forename: Jennifer
authors.initials: J
authors.lastname: Bryson
authors.forename: Brian
authors.initials: B
authors.lastname: Folsom
authors.forename: Chris
authors.initials: C
authors.lastname: Wilson
authors.forename: Jeff
authors.initials: J
authors.lastname: Duty
authors.forename: Albert A
authors.initials: AA
authors.lastname: Yen
authors.forename: Stanley H
authors.initials: SH
authors.lastname: Appel
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000097:blood* / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D015932:Complement C3c / Q000032:analysis*; D006801:Humans; D010300:Parkinson Disease / Q000097:blood* / Q000175:diagnosis*; D018570:Risk Assessment / Q000379:methods*; D012307:Risk Factors
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D015932:Complement C3c