title: Determination of nifedipine in serum of women in preterm labor by high-performance liquid chromatography with diode array detection.
abstract: Nifedipine (Nif) is widely used in treating cardiovascular disorders (especially hypertension) and for inhibiting preterm labor. A fully validated selective high-performance liquid chromatographic method with diode array detection, using solid-phase extraction, was developed for the determination of Nif in human serum. To assess specificity, Nif and its degradation products were separated on a Purospher RP-18 (5 microm, 125 x 4 mm) column plus a LiChrospher 100 RP-18 (5 microm, 4 x 4 mm) precolumn with a mobile phase of methanol-10 mM aqueous trifluoroacetic acid, pH 7.3 (57 + 43, v/v); chromatographic separation was followed by UV detection at 238 nm. For toxicological analysis, Nif in the presence of other calcium-channel antagonist drugs was identified under optimum chromatographic conditions. The calibration graph was constructed over the concentration range of 12.5-400 ng/mL in serum with good correlation (r = 0.9956). This method was not subject to interference by other plasma components and was successfully applied to the assay of Nif in spiked human serum and in serum of women in preterm labor after sublingual administration of 30 mg Nif per day divided into 3 equal doses. The mean recovery based on the ratio of the slopes of serum and mobile phase standard curves was 96.5%. The detection and quantification limits of the drug in spiked human serum were found to be 6 and 17.5 ng/mL, respectively. Validation of the method demonstrated good intraday and interday precision, which ranged from 2.18 to 6.67% and from 6.52 to 11.93%, respectively.
authors.affiliation: Medical University of Lublin, Faculty of Pharmacy, Department of Medicinal Chemistry, 6 Chodzki Str, 20-093 Lublin, Poland. dorkow@vp.pl
authors.forename: Dorota
authors.initials: D
authors.lastname: Kowalczuk
authors.forename: Maria Bozena
authors.initials: MB
authors.lastname: Wawrzycka
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Haratym Maj
mesh_terms: D002118:Calcium / Q000737:chemistry; D002121:Calcium Channel Blockers / Q000737:chemistry; D015220:Calcium Channels / Q000737:chemistry; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D000432:Methanol / Q000737:chemistry; D015233:Models, Statistical; D009529:Nicardipine / Q000494:pharmacology; D009543:Nifedipine / Q000097:blood* / Q000494:pharmacology; D009553:Nimodipine / Q000494:pharmacology; D009568:Nitrendipine / Q000494:pharmacology; D007752:Obstetric Labor, Premature; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014269:Trifluoroacetic Acid / Q000032:analysis; D014466:Ultraviolet Rays
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D009553:Nimodipine; D009568:Nitrendipine; D009529:Nicardipine; D014269:Trifluoroacetic Acid; D009543:Nifedipine; D002118:Calcium; D000432:Methanol