Transformation of undomesticated strains of Bacillus subtilis by protoplast electroporation.
A rapid method combining the use of protoplasts and electroporation was developed to transform recalcitrant wild strains of Bacillus subtilis. The method described here allows transformation with both replicative and integrative plasmids, as well as with chromosomal DNA, and provides a valuable tool for molecular genetic analysis of interesting Bacillus strains, which are hard to transform by conventional methods.
Romero
Diego
D
Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos s/n, E-29071 Málaga, Spain.
Pérez-García
Alejandro
A
Veening
Jan-Willem
JW
de Vicente
Antonio
A
Kuipers
Oscar P
OP
D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology*; D018274:Electroporation / Q000379:methods*; D011523:Protoplasts / Q000502:physiology*; D014170:Transformation, Genetic / Q000502:physiology*