title: [Azoospermia factor microdeletions in idiopathic azoospermia and severe oligozoospermia].
abstract: OBJECTIVE
  To observe the relationship between microdeletions of AZF( azoospermia factor) on Y chromosome in male with idiopathic azoospermia and severe oligozoospermia.
  
  
  METHODS
  Only patients with an apparently normal 46,XY karyotype and normal FSH, LH and T were included in this study. Multiplex PCR was used to detect the sequence-tagged sites( STS) as follows :sY84, sY86, sY127, sY134, sY152, sY153, sY254, sY255, and ZFX/Y was used as internal control gene.
  
  
  RESULTS
  No microdeletion was detected in the control whereas 8 microdeletion cases existed in 67 idiopathic azoospermia and severe oligozoospermia, including 4 in AZFc, 2 in AZFa + AZFc, 1 in AZFc + AZFb, and 1 in AZFb. The prevalence rate of microdeletion was 11.94%, which was statistically different from the control.
  
  
  CONCLUSIONS
  Microdeletions in the AZF regions on the long arm of the Y-chromosome are associated with idiopathic azoospermic and severely oligozoospermic men. Multiplex PCR was a rapid and reliable method for screening microdeletions of AZF.
authors.affiliation: Key Laboratory for Improving Birth Outcome Technique of Shandong Provincial Institute of Family Planning Resaerch, Jinan, Shandong 250002, China. jyf690629@163.com
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Jia
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Wu
authors.forename: Yi
authors.initials: Y
authors.lastname: Qiu
authors.forename: Ji-Ying
authors.initials: JY
authors.lastname: Qu
authors.forename: Chang-Zheng
authors.initials: CZ
authors.lastname: Song
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y; D056426:Genetic Loci; D006801:Humans; D007621:Karyotyping; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D016133:Polymerase Chain Reaction; D029589:Seminal Plasma Proteins / Q000235:genetics*
chemical_list: D029589:Seminal Plasma Proteins