Evaluation of an ELISA for detection of anti-parietal cell antibody.
OBJECTIVE
Anti-parietal cell antibody (APCA) is used for diagnosis of pernicious anemia and type A gastritis. APCA is present in patients with Helicobacter pylori (H. pylori)-positive gastritis and is related to atrophic gastritis and gastric carcinoma. The aim of the study was to determine whether an enzyme-linked immunosorbent assay (ELISA) can be used to detect APCA.


METHODS
Study subjects were 134 patients (74 men, 60 women; mean age, 63 years; range, 18-88 years) with gastric carcinoma, gastric ulcer, duodenal ulcer, gastritis, type A gastritis or pernicious anemia and H. pylori-negative normal mucosa subjects in the stomach. APCA was measured in all subjects by our ELISA method. Results of ELISA were compared with results of indirect immunofluorescence (IIF).


RESULTS
The percentage of samples that were APCA-positives by IIF at a 1/20 or greater serum dilutions was similar to that by ELISA (86.9%). Agreement between ELISA and IIF for presence of APCA was 78.3% in patients with gastric carcinoma, 80.1% (gastric ulcer), 59.1% (duodenal ulcer), 51.7% (gastritis), 75.0% (type A gastritis), 100% (pernicious anemia) and 80.0% (normal).


CONCLUSIONS
ELISA is a useful method for detection of APCA for diagnosis of PA and type A gastritis.
Sugiu
Kuniaki
K
Division of Gastroenterology, Department of Internal Medicine, Kawasaki Medical School, Kurashiki, Japan.
Kamada
Tomoari
T
Ito
Masanori
M
Kaya
Shunji
S
Tanaka
Aki
A
Kusunoki
Hiroaki
H
Hata
Jiro
J
Haruma
Ken
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D008875:Middle Aged; D010295:Parietal Cells, Gastric / Q000276:immunology*
D001323:Autoantibodies