The early dark-response in Arabidopsis thaliana revealed by cDNA microarray analysis.
Despite intense research on light responses in plants, the consequences of a simple shift from light to darkness remain poorly characterized. We have examined the transcriptome of Arabidopsis thaliana seedling leaves upon a shift from constant light to darkness for between 1 and 8 h, while excluding most effects associated with circadian oscillation. Expression clustering and gene ontology analyses identified about 790 responsive genes implicated in diverse cellular processes. Compared to the better-studied long-term dark adaptation response, the early response to darkness is partially overlapping yet clearly distinct, encompassing early transient, early sustained, and late response clusters. The repressor of photomorphogenesis, COP1 (constitutive photomorphogenic 1), is not a chief regulator of the early response to darkness, in contrast to its well-established role during long-term dark adaptation and etiolation. Only part of the early dark response can be understood as the opposite of the response following a dark-to-light transition and as a response to sugar deprivation. Bioinformatic comparisons with published microarray datasets further suggest that abscisic acid (ABA) signaling plays a prominent role in the early response to darkness, although this effect is not mediated by an increase in the ABA level. The potential basis for the co-regulation by darkness and ABA is discussed in light of sugar and redox signaling.
Kim
Byung-Hoon
BH
Department of Biochemistry, Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37996-0840, USA.
von Arnim
Albrecht G
AG
D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D015152:Blotting, Northern; D002241:Carbohydrates; D002940:Circadian Rhythm; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D018076:DNA, Complementary / Q000378:metabolism*; D003624:Darkness; D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D005821:Genetic Techniques; D005947:Glucose / Q000494:pharmacology; D006056:Golgi Apparatus / Q000378:metabolism; D008027:Light; D046228:Microarray Analysis; D008954:Models, Biological; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009991:Oscillometry; D010084:Oxidation-Reduction; D018521:Plant Physiological Phenomena; D012333:RNA, Messenger / Q000378:metabolism; D050436:Regulatory Elements, Transcriptional; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012542:Scattering, Radiation; D015398:Signal Transduction; D013997:Time Factors; D015854:Up-Regulation
D029681:Arabidopsis Proteins; D002241:Carbohydrates; D018076:DNA, Complementary; D012333:RNA, Messenger; D000040:Abscisic Acid; D005947:Glucose