Strong resistance of oligo(phosphorylcholine) self-assembled monolayers to protein adsorption.
In this work, we demonstrate the strong resistance of oligo(phosphorylcholine) (OPC) self-assembled monolayers (SAMs) to protein adsorption and cell adhesion. OPC SAMs were characterized using X-ray photoelectron spectroscopy (XPS), and protein adsorption was measured using a surface plasmon resonance (SPR) sensor. Results are compared with those of phosphorylcholine (PC) SAMs. Despite the existence of negative charge on OPC SAMs and the simple synthesis procedure of OPC thiols, OPC SAMs resist protein adsorption as effectively as or better than PC SAMs formed from highly purified PC thiols. The ease of their preparation and the effectiveness of their function make OPC SAMs an attractive alternative for creating nonfouling surfaces.
Chen
Shengfu
S
Department of Chemical Engineering, and Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.
Liu
Lingyun
L
Jiang
Shaoyi
S
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D010767:Phosphorylcholine / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D020349:Surface Plasmon Resonance
D011506:Proteins; D010767:Phosphorylcholine