Proteome of the bacterium Mycoplasma penetrans.
A proteome map of Mycoplasma penetrans has been constructed using two-dimensional gel electrophoresis in combination with mass spectrometry (MS). Mycoplasma penetrans infects the urogenital and respiratory tracts of humans. A total of 207 spots were characterized with MS and, in comparing the experimental data with the DNA sequence-derived predictions, it was possible to assign these 207 spots to 153 genes. The Pro-Q Diamond phosphoprotein dye technology was used for the fluorescent detection of 26 phosphoproteins in the 4-7 pH range.
Ferrer-Navarro
Mario
M
Institut de Biotecnología i de Biomedicina and Departament de Bioquímica i Biología Molecular, Universitat Autonoma de Barcelona. 08193, Bellaterra, Barcelona, Spain.
Gómez
Antonio
A
Yanes
Oscar
O
Planell
Raquel
R
Avilés
Francesc Xavier
FX
Piñol
Jaume
J
Pérez Pons
Josep Antoni
JA
Querol
Enrique
E
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D018661:Mycoplasma penetrans / Q000737:chemistry* / Q000235:genetics; D010449:Peptide Mapping; D020543:Proteome / Q000737:chemistry* / Q000235:genetics
D001426:Bacterial Proteins; D020543:Proteome