Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1.
Four amino-terminal immunoglobulin (Ig) modules and three fibronectin type III (F3) modules of the mouse neural cell-adhesion molecule L1 have been expressed in Drosophila S2 cells. The Ig modules I-IV of L1 crystallized in a trigonal space group, with unit-cell parameters a = b = 239.6, c = 99.3 A, and the crystals diffracted X-rays to a resolution of about 3.5 A. The F3 modules I-III of L1 crystallized in a tetragonal space group, with unit-cell parameters a = b = 80.1, c = 131 A, and the crystals diffracted X-rays to 2.8 A resolution. This is a step towards the structure determination of the multimodular constructs of the neural cell-adhesion molecule L1 in order to understand the function of L1 on a structural basis.
Kulahin
Nikolaj
N
Protein Laboratory, Institute of Molecular Pathology, Panum Institute, Blegdamsvej 3C, DK-2200 Copenhagen, Denmark. kulahin@plab.ku.dk
Kasper
Christina
C
Kristensen
Ole
O
Kastrup
Jette Sandholm
JS
Berezin
Vladimir
V
Bock
Elisabeth
E
Gajhede
Michael
M
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D005353:Fibronectins / Q000737:chemistry*; D015870:Gene Expression; D007136:Immunoglobulins / Q000737:chemistry*; D051379:Mice; D039842:Neural Cell Adhesion Molecule L1 / Q000737:chemistry*
D005353:Fibronectins; D007136:Immunoglobulins; D039842:Neural Cell Adhesion Molecule L1