Crystallization and preliminary X-ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase.
A thermostable thioredoxin reductase isolated from Sulfolobus solfataricus (SsTrxR) has been successfully crystallized in the absence and in the presence of NADP. Two different crystal forms have been obtained. Crystals of the form that yields higher resolution data (1.8 A) belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 76.77, b = 120.68, c = 126.85 A. The structure of the enzyme has been solved by MAD methods using the anomalous signal from the Se atoms of selenomethionine-labelled SsTrxR.
Ruggiero
Alessia
A
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, I-80134 Naples, Italy.
Ruocco
Maria Rosaria
MR
Grimaldi
Pasquale
P
Arcari
Paolo
P
Masullo
Mariorosario
M
Zagari
Adriana
A
Vitagliano
Luigi
L
D000244:Adenosine Diphosphate / Q000737:chemistry; D018360:Crystallography, X-Ray; D004583:Electrons; D004926:Escherichia coli / Q000378:metabolism; D008968:Molecular Conformation; D009249:NADP / Q000737:chemistry; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D012643:Selenium / Q000737:chemistry; D012645:Selenomethionine / Q000737:chemistry; D048229:Sulfolobus solfataricus / Q000201:enzymology*; D013696:Temperature; D013880:Thioredoxin-Disulfide Reductase / Q000737:chemistry*; D014961:X-Ray Diffraction
D011994:Recombinant Proteins; D009249:NADP; D000244:Adenosine Diphosphate; D012645:Selenomethionine; D013880:Thioredoxin-Disulfide Reductase; D012643:Selenium