title: [Preliminary study on adult odontoblast culture in situ].
abstract: OBJECTIVE
  To establish an in situ culture model of adult odontoblasts.
  
  
  METHODS
  Thirty intact and healthy third molars freshly prepared from 20-30 year old individuals were randomly divided into three groups. Each group had 10 molars. Group 1 was pulp tissue extraction group. Group 2 was serum-containing culture group. Group 3 was serum-free culture group. The root was dissected from the crown and the pulp was pulled out to make odontoblasts remaining in the crown. The odontoblasts were cultured in situ either in medium containing serum or serum-free medium for up to 7 days. The growth status of the cells was examined by light microscopy and cell morphology and distribution was analyzed by scanning electron microscopy. Cell viability was determined by trypan blue staining.
  
  
  RESULTS
  After pulp removal at room temperature, odontoblasts remained in the wall of the pulp chamber, and kept viable and good morphology during the 7-day culture.
  
  
  CONCLUSIONS
  We have successfully established an in situ culture model of adult primary odontoblasts in either serum-containing or serum-free medium.
authors.affiliation: Department of Oral Medicine, School of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai 200011, China. interdalao.student@sina.com
authors.forename: Xiao-chun
authors.initials: XC
authors.lastname: Wang
authors.forename: Jing-ping
authors.initials: JP
authors.lastname: Liang
authors.forename: Hao-yan
authors.initials: HY
authors.lastname: Li
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Zhang
authors.forename: Ai-mei
authors.initials: AM
authors.lastname: Song
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000328:Adult; D018929:Cell Culture Techniques; D003782:Dental Pulp; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008964:Molar, Third; D009804:Odontoblasts; D055815:Young Adult