[Role of keratynocytes in preservation of oral mucosa epithelium integrity. Part II].
The keratinocytes participate in all wound healing phases indirectly (secretion of polypeptide growth factors and enzymes) or directly (reepithelialization). In vitro culturing, and clinical application of keratinocytes in facilitating the process of wound healing have been the aim of many studies. The proliferation index of epithelial keratinocytes is higher than of those from the epidermis, in vitro epithelial cell differentiation is relatively slow and due to culturing it is possible to get an adequate quantity of material for transplantation procedure. A new method of culturing (an enzymatic method of cell isolation, serum free, and without feeder layer-mice's fibroblasts 3T3-method of culturing) allows to obtain epithelial cells with density required for transplantation in 10 days. The clinical application of Keratinocytes in Fibrin Glue Suspension (KFGS) on the wounded tissue allows to reinforce healing properties of both constituents (cells and fibrin matrix). Authors described two cases of KFGS application i.e.: in widening of attached gingiva, and in covering the wounded area after the excision of granuloma (granuloma fissuratum).
Joanna
Zarzecka
Z
Pracownia Stomatologii Zachowawczej z Endodoncja Instytutu Stomatologii Collegium Medicum, Uniwersytetu Jagiellońskiego w Krakowie.
Zapała
Jan
J
Drukała
Justyna
J
D000328:Adult; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000378:metabolism / Q000502:physiology*; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism* / Q000473:pathology; D010484:Periapical Granuloma / Q000378:metabolism / Q000473:pathology