A sensitive sandwich DNA array using fluorescent nanoparticle probes.
An ultrasensitive sandwich DNA array using highly fluorescent and photostable dyedoped silica nanoparticles is described. Compared to traditional sandwich arrays in which fluorophores have been used to signal target DNA molecules, the developed nanoparticle probes provide a much stronger fluorescent emission. Signal amplification of the dyedoped silica nanoparticles originates from the large number of dye molecules doped inside each individual nanoparticle. In addition, the silica matrix of the nanoparticles protects dye molecules from photobleaching. Thus, the dye-doped nanoparticles provide a constant fluorescent signal that is sufficient for detection of trace amounts of target DNA. By immobilizing a complementary DNA sequence to the target onto the nanoparticle surface, a fluorescent nanoparticle-DNA probe is formed. These nanoparticle probes are then used as superemitting reagents to perform a typical sandwich assay. By using a high-resolution fluorescent microscope, individual nanoparticle-DNA probes that have been hybridized to capture target strands can be observed clearly at low target DNA concentrations. More important, the number of the nanoparticle-DNA probes hybridized to the target DNA is proportional to the target DNA concentration in solution. By counting the number of localized fluorescent "spots" on the array, the target DNA concentration can be determined. In this chapter, detailed methods used to synthesize nanoparticle-DNA probes, fabricate the sandwich array, prepare the substrate, and quantitatively determine DNA concentration are described.
Zhao
Xiaojun
X
Department of Chemistry, University of North Dakota, Grand Forks, USA.
Pierce
David T
DT
Huan
Yanfu
Y
D005456:Fluorescent Dyes; D044085:Microfluidics; D015335:Molecular Probes; D049329:Nanostructures; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*
D005456:Fluorescent Dyes; D015335:Molecular Probes