Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: simultaneous binding to DNA phosphate backbone.
Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.
Zhao
Chunxia
C
Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
Dai
Qing
Q
Seino
Takehiro
T
Cui
Ying-Yu
YY
Nishizawa
Seiichi
S
Teramae
Norio
N
D000584:Amiloride / Q000737:chemistry*; D020029:Base Pairing; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D005456:Fluorescent Dyes; D006860:Hydrogen Bonding; D008024:Ligands; D010710:Phosphates / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013941:Thymine / Q000737:chemistry*
D005456:Fluorescent Dyes; D008024:Ligands; D010710:Phosphates; D000584:Amiloride; D004247:DNA; D013941:Thymine