Plasma cells and Fc receptors in human adipose tissue--lipogenic and anti-inflammatory effects of immunoglobulins on adipocytes.
We have previously reported high immunoglobulin expression in human omental adipose tissue. The aim of this work was to investigate plasma cell density and Fc receptor (FcR) expression in human adipose tissue depots and in vitro effects of immunoglobulins on adipocyte function. Plasma cell density was higher in the visceral compared to the subcutaneous depot (10.0+/-1.56% and 5.2+/-0.98%, respectively, n=20, p<0.05). Microarray analysis revealed expression of four FcR genes in adipose tissue; FCGR2A, FCGR2B, FCER1G, and FCGRT. FCGR2A was highly expressed in adipocytes in both depots and this was verified by immunohistochemistry. Expression of IL-1beta and IL-6 was markedly reduced in adipocytes after incubation with the Fc moiety of immunoglobulin G (Fc) (p<0.01). Furthermore, Fc stimulated adipocyte lipogenesis as potently as insulin (p<0.05), but did not influence lipolysis. In conclusion, immunoglobulins produced by plasma cells in human adipose tissue could influence adipocyte metabolism and cytokine production.
Palming
Jenny
J
Department of Metabolism and Cardiovascular Research, Institute of Medicine, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden.
Gabrielsson
Britt G
BG
Jennische
Eva
E
Smith
Ulf
U
Carlsson
Björn
B
Carlsson
Lena M S
LM
Lönn
Malin
M
D017667:Adipocytes / Q000276:immunology; D000273:Adipose Tissue / Q000166:cytology* / Q000276:immunology*; D000328:Adult; D002452:Cell Count; D016207:Cytokines / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D050356:Lipid Metabolism / Q000276:immunology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D009852:Omentum / Q000166:cytology; D010950:Plasma Cells / Q000276:immunology*; D011961:Receptors, Fc / Q000032:analysis* / Q000235:genetics*
D016207:Cytokines; D007136:Immunoglobulins; D011961:Receptors, Fc