title: Crystallization and preliminary X-ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase.
abstract: A thermostable thioredoxin reductase isolated from Sulfolobus solfataricus (SsTrxR) has been successfully crystallized in the absence and in the presence of NADP. Two different crystal forms have been obtained. Crystals of the form that yields higher resolution data (1.8 A) belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 76.77, b = 120.68, c = 126.85 A. The structure of the enzyme has been solved by MAD methods using the anomalous signal from the Se atoms of selenomethionine-labelled SsTrxR.
authors.affiliation: Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, I-80134 Naples, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Ruggiero
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Ruocco
authors.forename: Pasquale
authors.initials: P
authors.lastname: Grimaldi
authors.forename: Paolo
authors.initials: P
authors.lastname: Arcari
authors.forename: Mariorosario
authors.initials: M
authors.lastname: Masullo
authors.forename: Adriana
authors.initials: A
authors.lastname: Zagari
authors.forename: Luigi
authors.initials: L
authors.lastname: Vitagliano
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry; D018360:Crystallography, X-Ray; D004583:Electrons; D004926:Escherichia coli / Q000378:metabolism; D008968:Molecular Conformation; D009249:NADP / Q000737:chemistry; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D012643:Selenium / Q000737:chemistry; D012645:Selenomethionine / Q000737:chemistry; D048229:Sulfolobus solfataricus / Q000201:enzymology*; D013696:Temperature; D013880:Thioredoxin-Disulfide Reductase / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D011994:Recombinant Proteins; D009249:NADP; D000244:Adenosine Diphosphate; D012645:Selenomethionine; D013880:Thioredoxin-Disulfide Reductase; D012643:Selenium