title: Expression of costimulatory molecule CD80 in colonic dysplasia in ulcerative colitis: an immunosurveillance mechanism against colorectal cancer?
abstract: OBJECTIVE
  Ulcerative colitis is an established risk factor for colorectal cancer but dysplasia reports are much more frequent than invasive neoplasm diagnosis. The effective activation of T lymphocytes that provide antitumor surveillance requires the presence of costimulation molecules such as CD80 and CD86 on the surface of antigen-presenting cells. The aim of our study was to verify the presence of an in vivo immunosurveillance mechanism in the early stages of colon tumorigenesis.
  
  
  METHODS
  Expression of CD80, CD86, and IFN-gamma in the colonic mucosa of 21 consecutive ulcerative colitis (UC) patients was quantified using reverse transcription polymerase chain reaction. After a 7-year follow-up period, we reviewed the histology of all surveillance colonoscopy specimens for colonic dysplasia. Correlation, frequency, and survival analyses were performed.
  
  
  RESULTS
  CD80 was detectable in seven patients while expression of CD86 and IFN-gamma was evident in all patients. Histology confirmed the presence of dysplasia in eight patients. Patients who had dysplasia showed higher CD80 levels compared to those without dysplasia (p=0.02). Survival analysis demonstrated that cumulative dysplasia rates of CD80-positive patients were significantly higher than those of CD80-negative patients (p=0.04).
  
  
  CONCLUSIONS
  Even if partially limited by a relatively small sample size, our study seems to show an association between CD80 expression and colonic dysplasia in UC patients that may suggest a role for CD80 in the immunosurveillance against colorectal cancer in this early stage of tumorigenesis. On the contrary, CD86 seems to be involved in the inflammatory pathogenesis of UC.
authors.affiliation: Dipartimento di Scienze Chirurgiche e Gastroenterologiche, sez. di Clinica Chirurgica I, University of Padova, via Giustiniani 2, Padova 35128, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Scarpa
authors.forename: Roubik
authors.initials: R
authors.lastname: Behboo
authors.forename: Imerio
authors.initials: I
authors.lastname: Angriman
authors.forename: Attilio
authors.initials: A
authors.lastname: Cecchetto
authors.forename: Renata
authors.initials: R
authors.lastname: D'Incà
authors.forename: Barbara
authors.initials: B
authors.lastname: Termini
authors.forename: Michela
authors.initials: M
authors.lastname: Barollo
authors.forename: Cesare
authors.initials: C
authors.lastname: Ruffolo
authors.forename: Lino
authors.initials: L
authors.lastname: Polese
authors.forename: Giacomo Carlo
authors.initials: GC
authors.lastname: Sturniolo
authors.forename: Davide Francesco
authors.initials: DF
authors.lastname: D'Amico
mesh_terms: D000199:Actins / Q000032:analysis; D000293:Adolescent; D000328:Adult; D000368:Aged; D018122:B7-1 Antigen / Q000032:analysis*; D051940:B7-2 Antigen / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D003113:Colonoscopy; D015179:Colorectal Neoplasms / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D007413:Intestinal Mucosa / Q000737:chemistry / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015166:Monitoring, Immunologic; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis
chemical_list: D000199:Actins; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D014408:Biomarkers, Tumor; D007371:Interferon-gamma