[Effect of ethyl pyruvate on peroxidation injury of intestinal mucosa in rats with severe abdominal infection].
OBJECTIVE
To investigate the preventive effect of ethyl pyruvate (EP) on peroxidation injury to intestinal mucosa in rats with severe abdominal infection.


METHODS
Thirty-six SD rats were divided randomly into three groups (n=30 in each group): control group (laparotomy only), infection group [cecal ligation and puncture (CLP) was performed to reproduce severe abdominal infection model] and EP group (CLP plus 40 mg/kg EP subcutaneous injection, once per 8 hours). The changes in intestinal mucosa pathologic score were observed, and malondialdehyde (MDA) and myeloperoxidase (MPO) activities in intestinal tissue, and serum MDA levels were determined at postoperative 24 and 48 hours.


RESULTS
Inflammation of small intestine mucosa was more severe in the infection group than in EP group, and the pathologic scores were lower in EP group than those of the infection group at post-CLP 24 and 48 hours (all P<0.05). There was a significant positive correlation between the intestinal and plasma MDA in the infection group (r=0.867, P<0.05). The MDA and MPO levels in intestinal tissue and serum were higher in the infection group than in EP group and control group (all P<0.05).


CONCLUSIONS
With severe intraperitoneal infection in rats, the intestinal mucosa is damaged by the reactive oxygen species. EP could ameliorate the injury of intestinal mucosa by attenuating the injurious effects of the reactive oxygen species.
Li
Kun
K
Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China.
Wu
Cheng-tang
CT
Qiu
Xue-hong
XH
D034841:Abdominal Cavity; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007239:Infections / Q000378:metabolism / Q000473:pathology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011773:Pyruvates / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D011773:Pyruvates; C046522:ethyl pyruvate; D008315:Malondialdehyde; D009195:Peroxidase