title: Borrelia burgdorferi sensu stricto invasiveness is correlated with OspC-plasminogen affinity.
abstract: Borrelia burgdorferi sensu lato, the causative agent of Lyme borreliosis, is transmitted through tick bite. Lyme borreliosis evolves in two stages: a primary red skin lesion called erythema migrans; later on, invasive bacteria disseminate to distant sites inducing secondary manifestations (neuropathies, arthritis, carditis, late skin disorders). It has been previously suggested that the ospC gene could be associated with invasiveness in humans depending on its sequence. Here, we confirm the pattern of invasiveness, according to B. burgdorferi sensu stricto (B. b. ss) ospC group, using the mouse as an experimental host of B. b. ss. As it has been shown that the host plasminogen activation system is used by B. burgdorferi to disseminate throughout the host, we studied the interaction of plasminogen with OspC proteins from invasive and non-invasive groups of B. b. ss. Using two methods, ELISA and surface plasmon resonance, we demonstrate that indeed OspC is a plasminogen-binding protein. Moreover, significant differences in binding affinity for plasminogen are correlated with different invasiveness patterns in mice. These results suggest that the correlation between ospC polymorphism and Borrelia invasiveness in humans is linked, at least in part, to differences in OspC affinity for plasminogen.
authors.affiliation: Laboratoire des Spirochètes, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris Cedex 15, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Lagal
authors.forename: Denis
authors.initials: D
authors.lastname: Portnoï
authors.forename: Grazyna
authors.initials: G
authors.lastname: Faure
authors.forename: Danièle
authors.initials: D
authors.lastname: Postic
authors.forename: Guy
authors.initials: G
authors.lastname: Baranton
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D025065:Borrelia burgdorferi / Q000502:physiology*; D006801:Humans; D008193:Lyme Disease / Q000382:microbiology; D051379:Mice; D016513:Mice, SCID; D010958:Plasminogen / Q000378:metabolism*; D011485:Protein Binding; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; C079616:OspC protein; D010958:Plasminogen