title: Lipopolysaccharide heterogeneity among Burkholderia pseudomallei from different geographic and clinical origins.
abstract: Heterogeneous patterns were obtained for lipopolysaccharide (LPS) from 1,327 Burkholderia pseudomallei isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, silver staining, and immunoblot analysis. Two LPS serotypes (A and B) possessing different ladder profiles and a rough LPS without ladder appearances were identified. All three LPS types were antigenically distinct by immunoblotting. The predominant type A (97%) produced the lowest amount of biofilm. The two less common types (smooth type B and rough type) were found more in clinical than environmental isolates and more in Australian isolates than Thai isolates. These isolates were more often associated with relapse than with primary infection.
authors.affiliation: Faculty of Tropical Medicine, and Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand. narisara@tropmedres.ac
authors.forename: Narisara
authors.initials: N
authors.lastname: Anuntagool
authors.forename: Vanaporn
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: White
authors.forename: Bart J
authors.initials: BJ
authors.lastname: Currie
authors.forename: Rasana W
authors.initials: RW
authors.lastname: Sermswan
authors.forename: Surasakdi
authors.initials: S
authors.lastname: Wongratanacheewin
authors.forename: Suwimol
authors.initials: S
authors.lastname: Taweechaisupapong
authors.forename: Sansanee C
authors.initials: SC
authors.lastname: Chaiyaroj
authors.forename: Stitaya
authors.initials: S
authors.lastname: Sirisinha
mesh_terms: D000818:Animals; D000940:Antigenic Variation; D018441:Biofilms / Q000254:growth & development; D016957:Burkholderia pseudomallei / Q000378:metabolism* / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000737:chemistry / Q000145:classification* / Q000276:immunology / Q000378:metabolism; D008554:Melioidosis / Q000382:microbiology*
chemical_list: D008070:Lipopolysaccharides