Effect of orally administered probiotic E. coli strain Nissle 1917 on intestinal mucosal immune cells of healthy young pigs.
Several beneficial effects of probiotics have been described in studies using rodent disease models and in human patients; however, the underlying mechanisms remained mostly unclear. Only a few studies focused on the effects of probiotics on the intestinal mucosal immune system. Here, we studied the effect of the probiotic strain E. coli Nissle 1917 (EcN) administered orally to young pigs at two concentrations (10(9) and 10(11)CFU/d for 21 days) on the gut-associated lymphatic tissue. This probiotic strain was shown recently to reduce recurrence of inflammation in ulcerative colitis patients. We quantified the number and distribution of intestinal immune cells (granulocytes, mast cells, CD4+, CD8+, CD25+, IgA+ lymphocytes) and the mucosal mRNA expression of cytokines (IFN-gamma, TNF-alpha, TGF-beta, IL-10) and antimicrobial peptides (PR-39, NK-lysin, prepro-defensin-beta 1, protegrins). The number and distribution of cells were highly different between small intestinal and colon segments in all groups, but were not influenced by EcN, except high dose EcN fed pigs (10(11) CFU/d) showing an increase in mucosal CD8+ cells in the ascending colon. The mRNA analysis revealed no changes associated with EcN feeding. In conclusion, according to our analyses EcN has only minor effects on the distribution of mucosal immune cells in the gut of healthy individuals. The well-established preventive effects of EcN might therefore be relate to other mechanisms than simple modulation of immune cell distribution.
Duncker
Swantje C
SC
Department of Physiology, School of Veterinary Medicine, Bischofsholer Damm 15, 30173 Hannover, Germany. duncker@mcmaster.ca
Lorentz
Axel
A
Schroeder
Bernd
B
Breves
Gerhard
G
Bischoff
Stephan C
SC
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000276:immunology; D002452:Cell Count / Q000662:veterinary; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D023082:Defensins / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology*; D018928:Immunity, Mucosal / Q000187:drug effects*; D007150:Immunohistochemistry / Q000662:veterinary; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology*; D019936:Probiotics / Q000494:pharmacology*; D011510:Proteolipids / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000276:immunology*
D023181:Antimicrobial Cationic Peptides; D016207:Cytokines; D023082:Defensins; C093238:NK-lysin; D011510:Proteolipids; D012333:RNA, Messenger; C072212:PR 39