Effect of mouse Sim2 gene on the cell cycle of PC12 cells.
Sim2 gene plays an important role in the pathogenesis of Down syndrome (DS). To observe the effect of mouse Sim2 (mSim2) on the cell cycle of PC12 cells in vitro and explore the role of Sim2 in the pathogenesis of DS, we cloned the full open reading frame of mSim2 into the pcDNA3 vector and transfected it into PC12 cells, before analysing the effect of mSim2 on the cell cycle. A eukaryotic expression vector of mSim2 (pcDNA3-mSim2) was successfully constructed. There was notable expression of mSim2 mRNA in the cells transfected with pcDNA3-Sim2. Flow cytometry showed that there were more cells in G(0)/G(1) phase in the Sim2-transfected cells than that in the controls (P < 0.01), and significantly fewer in G(2)/M phase (P < 0.01). The mRNA and protein expressions of cyclin E decreased in the Sim2-transfected cells, while p27 expression increased significantly (P < 0.01). It is concluded that Sim2 may play an important role in the pathogenesis of DS by inhibiting the cell cycle, which is related to the decreased expression of cyclin E and increased expression of p27.
Meng
Xianfang
X
Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Shi
Jing
J
Peng
Bin
B
Zou
Xiaojing
X
Zhang
Chun
C
D000818:Animals; D001483:Base Sequence / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle; D002478:Cells, Cultured; D003001:Cloning, Molecular; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D016366:Open Reading Frames / Q000235:genetics; D016716:PC12 Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
D051792:Basic Helix-Loop-Helix Proteins; D019927:Cyclin E; D050760:Cyclin-Dependent Kinase Inhibitor p27; D012333:RNA, Messenger; C486578:Sim2 protein, mouse