[Changes in serum leptin levels in patients with surgically induced stress responses].
OBJECTIVE
To explore the effect of operative trauma induced stress responses on serum leptin levels.


METHODS
Serum samples of patients who had undergone resection of hepatic tumors or cholecystectomy were collected, and highly sensitive radioimmunoassay and enzyme-linked immunoadsorbent assay (ELISA) were used to determine serum levels of leptin, granulocyte-clone stimulating factor (G-CSF), C-reactive protein (CRP) and adrenocorticotropin hormone (ACTH) in the blood of these patients.


RESULTS
Compared with self-control before operation, serum leptin levels decreased slightly right after an abdominal operation (T0), it reached the highest level 1 day after operation (T1), and began to decrease from 2 days (T2) to 4 days after operation (T4), but the level was still higher than that before operation. Serum leptin levels of patients undergoing laparoscopic operation showed no significant difference when compared with that of laparotomy patients. G-SF levels decreased significantly after operation in both groups, and didn't recover to the levels before operation from T1 to T4. CRP levels slightly decreased in both groups at T0, but increased significantly higher than the levels before operation from T1 to T4. ACTH levels of decreased significantly in laparotomy patients from T0 to T1, and began to recover on T2, while that of laparoscopic operation patients showed no significant difference before and after operation.


CONCLUSIONS
Serum leptin levels of patients increase significantly and constantly subsequent to operative trauma induced stress responses, but this change has no correlation with that of CRP, G-SF and ACTH.
Yan
Guang-tao
GT
Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of PLA, Beijing 100853, China. yan301@263.net
Hao
Xiu-hua
XH
Xue
Hui
H
Lin
Ji
J
Zhang
Kai
K
Wang
Lu-huan
LH
D000324:Adrenocorticotropic Hormone / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood; D006801:Humans; D020738:Leptin / Q000097:blood*; D011184:Postoperative Period; D013514:Surgical Procedures, Operative
D020738:Leptin; D016179:Granulocyte Colony-Stimulating Factor; D000324:Adrenocorticotropic Hormone; D002097:C-Reactive Protein