title: Assembly, activation, and trafficking of the Fet3p.Ftr1p high affinity iron permease complex in Saccharomyces cerevisiae.
abstract: The high affinity iron uptake complex in the yeast plasma membrane (PM) consists of the ferroxidase, Fet3p, and the ferric iron permease, Ftr1p. We used a combination of yeast two-hybrid analysis, confocal fluorescence microscopy, and fluorescence resonance energy transfer (FRET) quantification to delineate the motifs in the two proteins required for assembly and maturation into an uptake-competent complex. The cytoplasmic, carboxyl-terminal domain of each protein contains a four-residue motif adjacent to the cytoplasm-PM interface that supports an interaction between the proteins. This interaction has been quantified by two-hybrid analysis and is required for assembly and trafficking of the complex to the PM and for the approximately 13% maximum FRET efficiency determined. In contrast, the Fet3p transmembrane domain (TM) can be exchanged with the TM domain from the vacuolar ferroxidase, Fet5p, with no loss of assembly and trafficking. A carboxyl-terminal interaction between the vacuolar proteins, Fet5p and Fth1p, also was quantified. As a measure of the specificity of interaction, no interaction between heterologous ferroxidase permease pairs was observed. Also, whereas FRET was quantified between fluorescent fusions of the copper permease (monomers), Ctr1p, none was observed between Fet3p and Ctr1p. The results are consistent with a (minimal) heterodimer model of the Fet3p.Ftr1p complex that supports the trafficking of iron from Fet3p to Ftr1p for iron permeation across the yeast PM.
authors.affiliation: Department of Biochemistry, School of Medicine and Biomedical Sciences, The University at Buffalo, Buffalo, New York 14214.
authors.forename: Arvinder
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Biochemistry, School of Medicine and Biomedical Sciences, The University at Buffalo, Buffalo, New York 14214.
authors.forename: Scott
authors.initials: S
authors.lastname: Severance
authors.affiliation: Department of Biochemistry, School of Medicine and Biomedical Sciences, The University at Buffalo, Buffalo, New York 14214.
authors.forename: Navjot
authors.initials: N
authors.lastname: Kaur
authors.affiliation: Department of Biochemistry, School of Medicine and Biomedical Sciences, The University at Buffalo, Buffalo, New York 14214.
authors.forename: William
authors.initials: W
authors.lastname: Wiltsie
authors.affiliation: Department of Biochemistry, School of Medicine and Biomedical Sciences, The University at Buffalo, Buffalo, New York 14214. Electronic address: camkos@buffalo.edu.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Kosman
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002570:Ceruloplasmin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D004789:Enzyme Activation; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D007501:Iron / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D021381:Protein Transport; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C098723:FTR1 protein, S cerevisiae; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D003300:Copper; D007501:Iron; D002570:Ceruloplasmin; C085513:FET3 protein, S cerevisiae