Involvement of oxidative stress induction in Na+ toxicity and its relation to the inhibition of a Ca2+ -dependent but calcineurin-independent mechanism in Saccharomyces cerevisiae.
Uridine 5'-hexadecylphosphate (UMPC16) inhibited the growth of Saccharomyces cerevisiae under a hypersaline stress condition with Na+ more strongly than the calcineurin inhibitor cyclosporine A (CsA). Additional Ca2+ supplementation similarly suppressed the inhibitory activities of UMPC16 and CsA on yeast cell growth in a medium with Na+. UMPC16, but not CsA, accelerated mitochondrial reactive oxygen species (ROS) generation in combination with Na+, suggesting its inhibition of a Ca2+ -dependent but calcineurin-independent mechanism for protection against Na+ toxicity.
Tanaka
Toshio
T
Department of Biology and Geosciences, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan. tanakato@sci.osaka-cu.ac.jp
Nishio
Kyoichi
K
Usuki
Yoshinosuke
Y
Fujita
Ken-Ichi
K
D065095:Calcineurin Inhibitors; D002118:Calcium / Q000494:pharmacology*; D016572:Cyclosporine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism; D009997:Osmotic Pressure; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000502:physiology*; D012964:Sodium / Q000037:antagonists & inhibitors / Q000633:toxicity*; D014542:Uridine Monophosphate / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology
D065095:Calcineurin Inhibitors; D017382:Reactive Oxygen Species; C409383:uridine 5'-hexadecylphosphate; D016572:Cyclosporine; D012964:Sodium; D014542:Uridine Monophosphate; D002118:Calcium