title: [Polymorphisms analysis of mitochondrial DNA in coding area].
abstract: OBJECTIVE
  To analyze mitochondrial DNA (mtDNA) polymorphisms in coding area and provide a theoretical basis for applying in forensic science.
  
  
  METHODS
  The primers of 8162F/8483R and 13070F/13299R were designed according to the Anderson's sequence. Using PCR-sequencing method to detect polymorphisms of mtDNA nt8162-8483 and nt13070-13299.
  
  
  RESULTS
  The lengths of the amplicons were 322 bp and 230 bp respectively. There were 24 mitochondrial haplotypes defined by 21 variable positions in both regions. The gene diversity was estimated at 0.751 1, and the probability of two randomly selected individuals having identical mtDNA types was 0.256 4.
  
  
  CONCLUSIONS
  The polymorphic sites within mtDNA coding area can be useful in combination with mtDNA control region in order to increase the discrimination power.
authors.affiliation: Forensic Medical Examination Center of Beijing Public Security Bureau, Beijing 100085, China. yachenglicc@163.com
authors.forename: Ya-cheng
authors.initials: YC
authors.lastname: Liu
authors.forename: Jin-ping
authors.initials: JP
authors.lastname: Hao
authors.forename: Jian-gwei
authors.initials: JG
authors.lastname: Yan
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: He
authors.initials: H
authors.lastname: Ren
authors.forename: Jia-cheng
authors.initials: JC
authors.lastname: Ren
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006238:Haploidy; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial