title: [Cloning and expression of soluble B lymphocyte stimulator].
abstract: OBJECTIVE
  To clone and express soluble B lymphocyte stimulator (sBLyS).
  
  
  METHODS
  Total RNA was isolated from peripheral blood mononuclear cells, and used to synthesize cDNA by reverse transcription. sBLyS cDNA was amplified by PCR with specific primers and inserted into a prokaryotic expression vector pET-30a. Recombinant plasmid was transformed into E.coli strain BL21(DE3). sBLyS was expressed in E.coli, purified in vitro, and analyzed with peptide mass fingerprinting and Daudi cell proliferation assay.
  
  
  RESULTS
  sBLyS cDNA was cloned. Peptide mass fingerprinting of purified BLyS matched with that of BLyS proteins. Purified sBLyS could stimulate Daudi cell proliferation in vitro.
  
  
  CONCLUSIONS
  sBLyS with biological activity was successfully expressed and purified.
authors.affiliation: Department of Molecular and Cellular Pharmacology, Tianjin University, Tianjin 300072, China. jsun@public3.bta.net.cn
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Jian-nan
authors.initials: JN
authors.lastname: Feng
authors.forename: Ying-xun
authors.initials: YX
authors.lastname: Sun
authors.forename: Mei-ru
authors.initials: MR
authors.lastname: Hu
authors.forename: Bei-feng
authors.initials: BF
authors.lastname: Shen
mesh_terms: D000818:Animals; D053264:B-Cell Activating Factor / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D005784:Gene Amplification; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry* / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: D053264:B-Cell Activating Factor; D018076:DNA, Complementary