Characterisation of Streptomyces spheroides NovW and revision of its functional assignment to a dTDP-6-deoxy-D-xylo-4-hexulose 3-epimerase.
Characterisation of recombinant Streptomyces spheroides NovW in vitro suggests that it is not a kinetically competent dual action dTDP-6-deoxy-d-xylo-4-hexulose 3,5-epimerase, but possesses only significant 3-epimerase activity.
Tello
Mónica
M
Centre for Carbohydrate Chemistry, University of East Anglia, Norwich, NR4 7TJ, UK.
Jakimowicz
Piotr
P
Errey
James C
JC
Freel Meyers
Caren L
CL
Walsh
Christopher T
CT
Buttner
Mark J
MJ
Lawson
David M
DM
Field
Robert A
RA
D002238:Carbohydrate Epimerases / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D007700:Kinetics; D008958:Models, Molecular; D015394:Molecular Structure; D009675:Novobiocin / Q000096:biosynthesis / Q000737:chemistry; D012997:Solvents; D013302:Streptomyces / Q000737:chemistry* / Q000201:enzymology*
D012997:Solvents; D009675:Novobiocin; D002238:Carbohydrate Epimerases