Genetic reproductive risk in inversion carriers.
OBJECTIVE
To evaluate the risk of four inversion carriers for producing unbalanced gametes.


METHODS
Prospective analysis of sperm nuclei by fluorescence in situ hybridization (FISH).


METHODS
Universitat Autònoma de Barcelona.


METHODS
Four inversion carriers.


METHODS
A semen sample from each patient was collected and prepared for FISH.


METHODS
The segregation outcome of each inversion was analyzed. The presence of interchromosomal effects (ICE) on chromosomes 13, 18, 21, X, and Y was also evaluated.


RESULTS
A variable production of unbalanced gametes, which implies a heterogeneous behavior of the inversions, was detected. This variability seems to be directly related to the size of the inversion, indicating that the production of recombinant gametes in inversion carriers would not be relevant when the inverted segment is smaller than 100 Mbp.


CONCLUSIONS
Inversions have a well-defined reproductive effect on carriers. Carriers of inversions up to 100 Mbp have a low [corrected] reproductive risk and would not usually benefit from preimplantation genetic diagnosis.
Anton
Ester
E
Unitat de Biologia Cellular, Facultat de Ciéncies, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Vidal
Francesca
F
Egozcue
Josep
J
Blanco
Joan
J
D000328:Adult; D007446:Chromosome Inversion; D020090:Chromosome Segregation; D002882:Chromosomes, Human, Pair 13; D002887:Chromosomes, Human, Pair 18; D002891:Chromosomes, Human, Pair 21; D004171:Diploidy; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008540:Meiosis; D008875:Middle Aged; D012730:Sex Chromosomes; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology