title: Genetic analysis of ADIPOR1 and ADIPOR2 candidate polymorphisms for type 2 diabetes in the Caucasian population.
abstract: Adiponectin is a metabolic link between adipose tissue and insulin action, mediating part of obesity-associated insulin resistance and type 2 diabetes. Two adiponectin receptors have been identified, and we investigated whether sequence variations in adiponectin receptor 1 (ADIPOR1) and adiponectin receptor 2 (ADIPOR2) genes could contribute to the genetic risk for type 2 diabetes in a case-control study of 1,498 Caucasian subjects. We sequenced the putative functional regions of the two genes in 48 subjects and selected single nucleotide polymorphisms (SNPs) from the public database. Five SNPs in ADIPOR1 and 12 in ADIPOR2 were tested for association with type 2 diabetes. No SNP of ADIPOR1 showed association in any of the samples from the French population. In contrast, three SNPs of ADIPOR2 showed nominal evidence for association with type 2 diabetes before correction for multiple testing (odds ratio [OR] 1.29-1.37, P = 0.034-0.014); only rs767870, located in intron 6, was replicated in an additional diabetes dataset (n = 636, OR 1.29, P = 0.020) with significant allelic association from the overall meta-analysis of 2,876 subjects (adjusted OR 1.25 [95% CI 1.07-1.45], P = 0.0051). In conclusion, our data suggest a modest contribution of ADIPOR2 variants in diabetes risk in the French population.
authors.affiliation: CNRS 8090, Institut de Biologie, Institut Pasteur de Lille, 1 rue du Professeur Calmette, BP 245, 59019 Lille, France. martine.vaxillaire@pasteur-lille.fr
authors.forename: Martine
authors.initials: M
authors.lastname: Vaxillaire
authors.forename: Aurélie
authors.initials: A
authors.lastname: Dechaume
authors.forename: Valérie
authors.initials: V
authors.lastname: Vasseur-Delannoy
authors.forename: Saida
authors.initials: S
authors.lastname: Lahmidi
authors.forename: Vincent
authors.initials: V
authors.lastname: Vatin
authors.forename: Frédéric
authors.initials: F
authors.lastname: Leprêtre
authors.forename: Philippe
authors.initials: P
authors.lastname: Boutin
authors.forename: Serge
authors.initials: S
authors.lastname: Hercberg
authors.forename: Guillaume
authors.initials: G
authors.lastname: Charpentier
authors.forename: Christian
authors.initials: C
authors.lastname: Dina
authors.forename: Philippe
authors.initials: P
authors.lastname: Froguel
mesh_terms: D000328:Adult; D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface / Q000235:genetics*; D044465:White People / Q000235:genetics*
chemical_list: C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface