293T cells expressing simian virus 40 T antigen are semi-permissive to bovine adenovirus type 3 infection.
Human cells do not normally support productive bovine adenovirus type 3 (BAdV-3) infection. Here, the outcome of BAdV-3 infection of both 293 cells and 293 cells modified to constitutively express the simian virus 40 (SV-40) T antigen (293T cells) was studied. Whereas BAdV-3 could efficiently infect 293 cells, there was a block in virus DNA replication, late-gene expression and virus production. In contrast, replication and efficient virus production could be detected in 293T cells infected with BAdV-3 or transfected with a replication-competent genomic BAdV-3 clone (pFBAV304). Early-phase gene expression was detected readily in both BAdV-3-infected 293 and 293T cells. However, the progression to efficient viral DNA synthesis and late-phase protein synthesis occurred only in 293T cells. Electron microscopy and virus growth kinetics demonstrated the formation of progeny virus in 293T cells. The SV-40 T antigens act to overcome a barrier in BAdV-3 DNA replication in 293 cells.
Patel
Amrutlal K
AK
Vectored Vaccine Program, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.
Tikoo
Suresh K
SK
Vectored Vaccine Program, Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D002460:Cell Line; D004261:DNA Replication; D006801:Humans; D017921:Mastadenovirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D008854:Microscopy, Electron; D014161:Transduction, Genetic; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
D000952:Antigens, Polyomavirus Transforming; D014764:Viral Proteins