Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.
The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.
Hunt
Dana E
DE
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, 48-421, 77 Massachusetts Ave., Cambridge, MA 02139, USA.
Klepac-Ceraj
Vanja
V
Acinas
Silvia G
SG
Gautier
Clement
C
Bertilsson
Stefan
S
Polz
Martin F
MF
D001419:Bacteria / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D015723:Gene Library; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D012338:RNA, Ribosomal, 23S