[Study on application of the whole genome amplification in LCN].
OBJECTIVE
To establish a method for whole genome amplification (WGA) based on (multiple displacement amplification MDA), and achieve DNA analysis of the human genome from low copy number (LCN).


METHODS
DNA sample was used for WGA according to the REPLI-g kit protocol (QIAGEN, Germany). WGA product was used for DNA analysis according to the Applied Biosystems Profiler Plus kit protocol (ABI, USA).


RESULTS
WGA product of DNA sample (10 pg) can be used for STR genotyping.


CONCLUSIONS
WGA technology could be helpful for LCN DNA analysis.
Zhou
Huai-gu
HG
Shanghai Institute of Criminal Science and Technology, Shanghai 200083,China.
Zhang
Chen
C
D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
D004247:DNA