title: Oxidation-reduction respiratory chains and ATP synthase complex are localized in detergent-resistant lipid rafts.
abstract: In order to detect and identify ubiquitous lipid raft marker proteins, we isolated lipid rafts from different mouse organs, including the liver, lung, large brain, and kidney, and analyzed their proteins via 2-DE. Many protein spots were determined to be ubiquitous in all of the lipid rafts, and were annotated via LC and MS/MS. Twelve proteins were identified as ubiquitous raft proteins, and most of these were determined to be mitochondrial proteins, including mortalin, prohibitin, voltage-dependent anion channel, ATP synthase, NADH dehydrogenase, and ubiquinol-cytochrome c reductase. Via immunoblotting, these proteins were shown to exist in detergent-resistant lipid rafts prepared using different organ tissues. Since these oxidation-reduction respiratory chains and ATP synthase complex were detected in detergent-resistant lipid raft fractions which had been isolated from the plasma membrane but not from the mitochondria, and found in the cell surface when determined by immunofluoresence and immunohistochemistry, we conclude that plasma membrane lipid rafts might contain oxidation-reduction respiratory chains and ATP synthase complex.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Anam-Dong, Sungbook-Ku, Seoul, Korea.
authors.forename: Ki-Bum
authors.initials: KB
authors.lastname: Kim
authors.forename: Joong-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Chang Seok
authors.initials: CS
authors.lastname: Lee
authors.forename: Bong-Woo
authors.initials: BW
authors.lastname: Kim
authors.forename: Hyo-Jung
authors.initials: HJ
authors.lastname: Choo
authors.forename: Soon-Young
authors.initials: SY
authors.lastname: Jung
authors.forename: Sung-Gil
authors.initials: SG
authors.lastname: Chi
authors.forename: Young-Sil
authors.initials: YS
authors.lastname: Yoon
authors.forename: Gyesoon
authors.initials: G
authors.lastname: Yoon
authors.forename: Young-Gyu
authors.initials: YG
authors.lastname: Ko
mesh_terms: D025181:ATP Synthetase Complexes / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002853:Chromatography, Liquid / Q000379:methods; D003902:Detergents / Q000494:pharmacology*; D004579:Electron Transport; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D013058:Mass Spectrometry / Q000379:methods; D021962:Membrane Microdomains / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D040901:Proteomics / Q000379:methods*
chemical_list: D003902:Detergents; D025181:ATP Synthetase Complexes