title: Chromohalobacter nigrandesensis sp. nov., a moderately halophilic, Gram-negative bacterium isolated from Lake Tebenquiche on the Atacama Saltern, Chile.
abstract: A total of 52 strains of moderately halophilic bacteria isolated from hypersaline sediment of Lake Tebenquiche on the Atacama Saltern, Chile, were subjected to a taxonomic study. The morphological, physiological, biochemical and nutritional characteristics of the strains matched those described for the genus Chromohalobacter. Cells were Gram-negative, non-spore-forming, rod-shaped and motile. A black pigmentation was produced. One strain, designated LTS-4N(T), grew optimally at 32 degrees C. Growth occurred in media containing 0.5-25% (w/v) total salts; the optimum was 7.5% (w/v) total salts. The pH range for growth was 5-10. The G + C content of the DNA of strain LTS-4N(T) was 59.8 mol%. Analysis of 16S rRNA gene sequence similarity revealed that strain LTS-4N(T) was closely related to Chromohalobacter species; however, DNA-DNA hybridization of representative strain LTS-4N(T) failed to associate the strain with any species of the genus Chromohalobacter with validly published names. Therefore, the name Chromohalobacter nigrandesensis sp. nov. is proposed. The type strain is LTS-4N(T) (= CECT 5315T = DSM 14323T).
authors.affiliation: Laboratory of Microbiology, 'Universidad Técnica Federico Santa María, Sede Viña del Mar', Viña del Mar, Chile.|Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, 18071 Granada, Spain.
authors.forename: Bernardo
authors.initials: B
authors.lastname: Prado
authors.affiliation: Department of Medical Technology, 'Facultad de Ciencias de la Salud, Universidad de Antofagasta', Av. Jaime Guzmán s/n, Antofagasta, Chile.|Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, 18071 Granada, Spain.
authors.forename: Catherine
authors.initials: C
authors.lastname: Lizama
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, 18071 Granada, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Aguilera
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, 18071 Granada, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Ramos-Cormenzana
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, 18071 Granada, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Fuentes
authors.affiliation: Laboratory of Environmental Microbiology, Catholic University of Valparaiso, Valparaíso, Chile.
authors.forename: Victoriano
authors.initials: V
authors.lastname: Campos
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, 18071 Granada, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Monteoliva-Sánchez
mesh_terms: D002677:Chile; D004275:DNA, Ribosomal / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology*; D044129:Halomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S