title: A new solid phase extraction clean-up method for the determination of 12 type A and B trichothecenes in cereals and cereal-based food by LC-MS/MS.
abstract: A new reliable and cost-efficient solid phase extraction-based clean-up method for the determination of 12 type A and B trichothecenes [deoxynivalenol (DON), nivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, fusarenon-X, T-2 toxin, HT-2 toxin, neosolaniol, monoacetoxy-scirpenol, diacetoxyscirpenol, T-2 triol and T-2 tetraol] in cereals and cereal-based food is presented. Furthermore, the suitability for the simultaneous determination of zearalenone is examined. Toxins were extracted from cereal samples using ACN/water (80/20, v/v), purified by means of a new Bond Elut Mycotoxin column and analyzed via liquid chromatography-electrospray ionization tandem mass spectrometry. Limits of detection were calculated for the matrix wheat and ranged from 0.3 to 5 ng/g, depending on the toxin. Average recovery rates for the tested compounds in seven cereal-based matrices have been determined ranging from 65 to 104%. The relative standard deviations of the complete method ranged from 2.67 (DON, wheat) to 20.0% (T-2 toxin, oats).
authors.affiliation: Chemisches und Veterinäruntersuchungsamt Stuttgart, Fellbach, Germany.
authors.forename: Marianna
authors.initials: M
authors.lastname: Klötzel
authors.forename: Uwe
authors.initials: U
authors.lastname: Lauber
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Humpf
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D005670:Fusarium / Q000378:metabolism; D007202:Indicators and Reagents; D011042:Poisons / Q000032:analysis*; D015203:Reproducibility of Results; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014255:Trichothecenes / Q000032:analysis*; D015025:Zearalenone / Q000032:analysis
chemical_list: D007202:Indicators and Reagents; D011042:Poisons; D012997:Solvents; D014255:Trichothecenes; D015025:Zearalenone