Expression, purification, crystallization and preliminary crystallographic analysis of the PAB0955 gene product.
PAB0955 from Pyrococcus abyssi is a prototype of a new Walker-type ATPase/GTPase conserved in archaea and eukaryota but not found in bacteria. PAB0955 has been expressed, purified and crystallized, and it has been shown that this thermostable protein is dimeric in reductive conditions. Crystals have been obtained either without nucleotide or in the presence of GDP or GTPgammaS. Preliminary X-ray crystallographic data up to 2.08 A resolution have been collected from these crystals.
Gras
Stéphanie
S
Laboratoire de Cristallographie et Cristallogénèse des Protéines, Institut de Biologie Structurale J.-P. Ebel, CEA-CNRS-UJF, 41 Rue Jules Horowitz, F-38027 Grenoble, France.
Fernandez
Bernard
B
Chaumont
Valérie
V
Carpentier
Philippe
P
Armengaud
Jean
J
Housset
Dominique
D
D017766:Acid Anhydride Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D018360:Crystallography, X-Ray; D017931:DNA Primers; D019641:DNA, Archaeal / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry; D019711:Pyrococcus / Q000737:chemistry* / Q000235:genetics
D001426:Bacterial Proteins; D017931:DNA Primers; D019641:DNA, Archaeal; D010446:Peptide Fragments; D017766:Acid Anhydride Hydrolases