Determination of mitiglinide in rat plasma by high-performance liquid chromatography with UV detection.
A selective and sensitive high-performance liquid chromatography method has been developed and validated for determination of mitiglinide (MGN) in rat plasma using 2-(4-biphenylyl) propionic acid (BPA) as internal standard. Liquid-liquid extraction was used for sample preparation. Chromatographic separation was achieved on a C(18) column using acetonitrile and 0.02 mol/l KH(2)PO(4) buffer (pH 4.0) (45:55, v/v) as mobile phase delivered at 1.0 ml/min. The UV detector was set at 210 nm. The assay was linear over the range 0.1-20 microg/ml for MGN. The average extraction recoveries of MGN and BPA from rat plasma were 98.6 and 97.4%, respectively. The developed method has been applied to the pharmacokinetic study of MGN in rats.
Lushan
Yu
Y
Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310031, China.
Su
Zeng
Z
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007004:Hypoglycemic Agents / Q000097:blood*; D007211:Indoles / Q000097:blood*; D054833:Isoindoles; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D007004:Hypoglycemic Agents; D007211:Indoles; D054833:Isoindoles; C087255:mitiglinide