[Analysis of point mutations in BRCA1 gene using hybridization on hydrogel microchips].
Germ-line mutations in BRCA1 gene account for a substantial proportion of inherited breast and ovarian cancers. Identification of these mutations allows molecular diagnosis for breast cancer susceptibility. We have developed method for identification of 185delAG, 300T>G, 4153delA, 4158A>G and 5382insC mutations in BRCA1 gene using hybridization with microarray of geL-immobilized oligonucleotides (microchip). The microchip was tested with 36 control samples, carrying the above-mentioned mutations and 65 clinical cases with breast cancer. Our data demonstrated that developed microchip can be very effective and realible tool, easily introduced in ordinary medical and genetic laboratories.
Fedorova
O E
OE
Sinitska
O N
ON
Tikhomirova
L P
LP
Vishnevskaia
Ia V
IaV
Zasedatelev
A S
AS
Nasedkina
T V
TV
D000328:Adult; D000368:Aged; D019313:BRCA1 Protein / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D020100:Hydrogels; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D017354:Point Mutation
D019313:BRCA1 Protein; D020100:Hydrogels