Molecular diagnosis of Bordetella pertussis infection by evaluation of formalin-fixed tissue specimens.
Formalin-fixed lung or trachea tissue specimens from four infants and one adolescent who died of respiratory illness were tested for Bordetella pertussis by conventional and real-time PCR assays. B. pertussis was confirmed in all cases. PCR can be an invaluable retrospective diagnostic tool for evaluating archival tissues from patients with suspected fatal pertussis.
Tatti
Kathleen M
KM
Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Viral and Rickettsial Disease, Infectious Disease Pathology Activity, 1600 Clifton Rd., NE, Mailstop G30, Atlanta, Georgia 30333, USA. ket2@cdc.gov
Wu
Kai-Hui
KH
Sanden
Gary N
GN
Greer
Patricia
P
Sumner
John
J
Guarner
Jeannette
J
Paddock
Christopher D
CD
Zaki
Sherif R
SR
D000293:Adolescent; D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D007223:Infant; D008168:Lung / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D016707:Tissue Fixation; D014917:Whooping Cough / Q000175:diagnosis* / Q000382:microbiology*
D004269:DNA, Bacterial; D005404:Fixatives; D005557:Formaldehyde