Amino acid sequence of RTX-A's isoform actinoporin from the sea anemone, Radianthus macrodactylus.
The amino acid sequence of actinoporin RTX-A (175 aa) from the sea anemone Radianthus macrodactylus was determined by sequencing of clones obtained via amplification of cDNA. It was established that RTX-A possessed high homology with HmgIII from Heteractis magnifica (87%) and StI, StII from Stichodactyla helianthus (84 and 87%, respectively). The analysis of structural and functional relationships within RTX-A was carried out. The some disagreement concerning to significant role of several amino acid residues for actinoporins exhibition of hemolytic activity was found.
Il'ina
Anna
A
Pacific Institute of Bioorganic Chemistry, Far East Branch, Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russian Federation. a.ilyina@gmail.com
Lipkin
Aleksey
A
Barsova
Ekaterina
E
Issaeva
Marina
M
Leychenko
Elena
E
Guzev
Konstantin
K
Monastyrnaya
Margarita
M
Lukyanov
Sergey
S
Kozlovskaya
Emma
E
D000595:Amino Acid Sequence; D000818:Animals; D003064:Cnidarian Venoms / Q000737:chemistry*; D003603:Cytotoxins / Q000737:chemistry*; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry; D012615:Sea Anemones / Q000737:chemistry*; D016415:Sequence Alignment
D003064:Cnidarian Venoms; D003603:Cytotoxins; D020033:Protein Isoforms