Liquid chromatography determination of citalopram enantiomers using beta-cyclodextrin as a chiral mobile phase additive.
A reliable and specific method for the determination of citalopram enantiomers was developed and validated. Chromatographic resolution of citalopram enantiomers was made on a Shim-pack (5 microm particle size) cyanopropyl column with beta-cyclodextrin (beta-CD) as an effective chiral mobile phase additive. The composition of the mobile phase was (90 + 10, v/v) aqueous 0.1% triethylammonium acetate buffer, pH 4.0 (adjusted with acetic acid), and acetonitrile, containing 12 mM beta-CD. The flow rate was 0.8 mL/min with ultraviolet detection at 240 nm. The effects of the mobile phase composition, concentration of beta-CD, and pH of the triethylammonium acetate buffer on peak shape and resolution of the enantiomers were investigated. The calibration graphs were linear (r = 0.9999, n = 8) in the range of 1-40 microg/mL for S(+) citalopram and R-(-) citalopram. The limit of detection values were 5.51 x 10(-3) and 4.35 x 10(-3) pg/mL, while the limit of quantification values were found to be 1.84 x 10(-2) and 1.45 x 10(-2) microg/mL for S-(+) citalopram and R-(-) citalopram, respectively.
El-Gindy
Alaa
A
Suez Canal University, Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Ismailia 41522, Egypt.
Emara
Samy
S
Mesbah
Mostafa K
MK
Hadad
Ghanda M
GM
D019342:Acetic Acid / Q000737:chemistry; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002845:Chromatography / Q000379:methods; D002853:Chromatography, Liquid; D015283:Citalopram / Q000032:analysis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology; D013237:Stereoisomerism; D013997:Time Factors; D014466:Ultraviolet Rays; D047392:beta-Cyclodextrins / Q000737:chemistry*
D019342:Acetic Acid; D015283:Citalopram; D000644:Quaternary Ammonium Compounds; D017367:Selective Serotonin Reuptake Inhibitors; D047392:beta-Cyclodextrins; C031215:betadex