title: Effect of recurrent laryngeal nerve section on the laryngeal muscles of adult rabbits.
abstract: OBJECTIVE
  To examine the effect of recurrent laryngeal nerve section on myonuclear addition and myofiber remodeling in adult rabbit laryngeal muscles.
  
  
  METHODS
  Unilateral section of the recurrent laryngeal nerve was performed on adult rabbits. Rabbits were labeled with bromodeoxyuridine, euthanized, and the laryngeal muscles were examined for bromodeoxyuridine (brdU) and neonatal myosin heavy chain (MyHC) expression.
  
  
  RESULTS
  One week after nerve section, brdU-positive myonuclei were significantly increased on the denervated and contralateral sides. Neonatal MyHC expression significantly increased. By 24 weeks, the upregulation of myonuclear addition returned to control levels.
  
  
  CONCLUSIONS
  Laryngeal muscle denervation results in significant upregulation of myonuclear addition. Denervation also resulted in a significant upregulation of neonatal MyHC expression.
  
  
  CONCLUSIONS
  Laryngeal muscles can be successfully reinnervated after long periods of denervation. This significant and maintained increase in myonuclear addition may contribute to the ability of laryngeal muscles to survive for extended periods following interruption of the recurrent laryngeal nerve.
authors.affiliation: Department of Otolaryngology, University of Minnesota, Rm. 374 Lions Research Building, 2001 6th Street SE, Minneapolis, MN 55455, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Shinners
authors.forename: George S
authors.initials: GS
authors.lastname: Goding
authors.forename: Linda K
authors.initials: LK
authors.lastname: McLoon
mesh_terms: D000818:Animals; D000963:Antimetabolites; D001973:Bromodeoxyuridine; D002467:Cell Nucleus / Q000648:ultrastructure; D016189:Dystrophin / Q000032:analysis; D007821:Laryngeal Muscles / Q000294:innervation* / Q000473:pathology / Q000648:ultrastructure; D009121:Muscle Denervation; D018485:Muscle Fibers, Skeletal / Q000648:ultrastructure; D018995:Myosin Heavy Chains / Q000032:analysis; D011817:Rabbits; D012009:Recurrent Laryngeal Nerve / Q000601:surgery*; D012038:Regeneration / Q000502:physiology; D012508:Sarcolemma / Q000648:ultrastructure; D015854:Up-Regulation
chemical_list: D000963:Antimetabolites; D016189:Dystrophin; D018995:Myosin Heavy Chains; D001973:Bromodeoxyuridine