Human RFP2 gene promoter: unique structure and unusual strength.
Human gene RFP2 is a candidate tumor suppressor located at 13q14.3 and deleted in multiple tumor types. To explore regulation of RFP2, we determined structure of the 5'-untranslated region of RFP2 gene and its promoter. RFP2 promoter area is TATA-less, highly enriched in G and C nucleotides, and contains multiple quadruplex forming GGGGA-repeats. Deletion analysis of 5'-flanking sequences demonstrated that repeat containing fragment possesses activity seven times exceeding that of the combined SV40 promoter/enhancer. Other unusual features of the RFP2 promoter include anomalously high electrostatic fields induced by sequence-dependent dipoles and very low nucleosome forming potential. A "minimized" version of the RFP2 promoter could be used for overexpression of the various transgenes in the mammalian cells.
Skoblov
Mikhail
M
Russian Center of Medical Genetics, Russian Academy of Medical Sciences, Moscow, Russia.
Shakhbazov
Konstantin
K
Oshchepkov
Dmitry
D
Ivanov
Dmitry
D
Guskova
Anna
A
Ivanov
Dmitry
D
Rubtsov
Petr
P
Prasolov
Vladimir
V
Yankovsky
Nick
N
Baranova
Ancha
A
D001483:Base Sequence; D019295:Computational Biology; D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics*; D020224:Expressed Sequence Tags; D054856:G-Quadruplexes; D005786:Gene Expression Regulation / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D004268:DNA-Binding Proteins; D009707:Nucleosomes; C112217:TRIM13 protein, human; D025521:Tumor Suppressor Proteins; D004247:DNA