title: [Cloning of the conserved fragment of urease gene of Staphylococcus warneri].
abstract: OBJECTIVE
  To obtain the clone of conserved fragment of urease gene of Staphylococcus warneri.
  
  
  METHODS
  Urease activity was measured by phenol red assay. An internal fragment of the urease gene of Staphylococcus warneri was amplified by PCR with primers derived from conserved amino acid sequences of the large subunit of urease. The PCR product was then cloned into T-vector and sequenced.
  
  
  RESULTS
  Urease activity of Staphylococcus warneri was positive. The internal fragment of urease gene of Staphylococcus warneri was highly homologous to known urease gene cluster of other bacteria.
  
  
  CONCLUSIONS
  In this study, urease activity of Staphylococcus warneri was proved and the internal fragment of urease gene encoding conserved amino acid sequences was cloned.
authors.affiliation: Department of Preventive and Pediatric Dentistry, School of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xi-ping
authors.initials: XP
authors.lastname: Feng
authors.forename: Ming-yu
authors.initials: MY
authors.lastname: Li
authors.forename: Zheng-wei
authors.initials: ZW
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D013210:Staphylococcus / Q000235:genetics*; D014510:Urease / Q000235:genetics*
chemical_list: D014510:Urease