title: [Study on application of the whole genome amplification in LCN].
abstract: OBJECTIVE
  To establish a method for whole genome amplification (WGA) based on (multiple displacement amplification MDA), and achieve DNA analysis of the human genome from low copy number (LCN).
  
  
  METHODS
  DNA sample was used for WGA according to the REPLI-g kit protocol (QIAGEN, Germany). WGA product was used for DNA analysis according to the Applied Biosystems Profiler Plus kit protocol (ABI, USA).
  
  
  RESULTS
  WGA product of DNA sample (10 pg) can be used for STR genotyping.
  
  
  CONCLUSIONS
  WGA technology could be helpful for LCN DNA analysis.
authors.affiliation: Shanghai Institute of Criminal Science and Technology, Shanghai 200083,China.
authors.forename: Huai-gu
authors.initials: HG
authors.lastname: Zhou
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
mesh_terms: D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D004247:DNA