title: Crystallization of a truncated soluble human semicarbazide-sensitive amine oxidase.
abstract: Human semicarbazide-sensitive amine oxidase (SSAO) is a homodimeric copper-containing monoamine oxidase that occurs in both a membrane-bound and a soluble form. SSAO is also known as vascular adhesion protein-1 (VAP-1). A truncated soluble form of human SSAO (comprising residues 29-763) was expressed in human embryonic kidney 293 cells and purified to homogeneity. Tetragonal crystals were obtained and a data set extending to 2.5 A was collected. The crystals are merohedrally twinned and the estimation of the twinning fraction was complicated by pseudo-symmetry and the anisotropic character of the crystals. Using a recently developed method for twinning detection that is insensitive to phenomena such as anisotropy or pseudo-symmetry [Padilla & Yeates (2003), Acta Cryst. D59, 1124-1130], the twinning fraction was estimated to be 0.3. The structure was eventually solved by molecular replacement in space group P4(3).
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University, Biomedical Centre, Box 596, SE-751 24 Uppsala, Sweden.
authors.forename: Emma
authors.initials: E
authors.lastname: Jakobsson
authors.forename: Joakim
authors.initials: J
authors.lastname: Nilsson
authors.forename: Ulla
authors.initials: U
authors.lastname: Källström
authors.forename: Derek
authors.initials: D
authors.lastname: Ogg
authors.forename: Gerard J
authors.initials: GJ
authors.lastname: Kleywegt
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D002460:Cell Line; D003460:Crystallization; D019281:Dimerization; D005822:Genetic Vectors; D006801:Humans; D007668:Kidney / Q000196:embryology; D008322:Mammals; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D017384:Sequence Deletion; D014162:Transfection; D014961:X-Ray Diffraction
chemical_list: D011994:Recombinant Proteins; D006631:Amine Oxidase (Copper-Containing)