title: Scale-up of L-lactic acid production by mutant strain Rhizopus sp. MK-96-1196 from 0.003 m3 to 5 m3 in airlift bioreactors.
abstract: In order to study the feasibility of commercial-scale L-lactic acid production by Rhizopus sp. MK-96-1196 using large scale airlift bioreactors (ALBs), a scale-up study from 0.003 m3 to 5 m3 ALB was carried out using oxygen transfer rate (OTR) as the scale-up criterion. Enhanced L-lactic acid production was achieved at OTRs higher than 0.28 (g-O2/l/h) irrespective of the scale of the bioreactor in question: in the range of 0.003 m3 to 5 m3, more than 90 g/lL-lactic acid was produced with a yield of approximately 80%, based on the initial glucose concentration. In future research, we plan to design an ALB greater than 3000 m3 (working volume: 2000 m3) for further studies on the production of L-lactic acid in large quantities.
authors.affiliation: The United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Liu
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Miura
authors.forename: Masaaki
authors.initials: M
authors.lastname: Yaguchi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Arimura
authors.forename: Enock Y
authors.initials: EY
authors.lastname: Park
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Okabe
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D019344:Lactic Acid / Q000096:biosynthesis*; D010100:Oxygen / Q000494:pharmacology; D012233:Rhizopus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D019344:Lactic Acid; D010100:Oxygen