title: A putative polyketide-synthesis protein XC5357 from Xanthomonas campestris: heterologous expression, crystallization and preliminary X-ray analysis.
abstract: Xanthomonas campestris pv. campestris (Xcc) is a Gram-negative yellow-pigmented bacterium and is the causative agent of black rot, one of the major worldwide diseases of cruciferous crops. It also synthesizes a variety of polyketide metabolites that lead to important antibiotics. XC5357 is a putative 12.2 kDa protein of unknown structure from Xcc that is likely to be essential for polyketide synthesis. It was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals belong to the triclinic space group P1, with unit-cell parameters a = 43.7, b = 43.7, c = 46.5 A, alpha = 65.0, beta = 64.9, gamma = 73.4 degrees, and diffracted to a resolution of 1.85 A.
authors.affiliation: Institute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan.
authors.forename: Chiao-Li
authors.initials: CL
authors.lastname: Chu
authors.forename: Ko-Hsin
authors.initials: KH
authors.lastname: Chin
authors.forename: Fu-Yang
authors.initials: FY
authors.lastname: Lin
authors.forename: Chia-Cheng
authors.initials: CC
authors.lastname: Chou
authors.forename: Cheng-Chung
authors.initials: CC
authors.lastname: Lee
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Shr
authors.forename: Ping-Chiang
authors.initials: PC
authors.lastname: Lyu
authors.forename: Andrew H-J
authors.initials: AH
authors.lastname: Wang
authors.forename: Shan-Ho
authors.initials: SH
authors.lastname: Chou
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D048630:Polyketide Synthases / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D016959:Xanthomonas campestris / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D048630:Polyketide Synthases