Mouse plasmacytoid dendritic cells derive exclusively from estrogen-resistant myeloid progenitors.
Current models predict that mouse plasmacytoid dendritic cells (PDCs) derive from lymphoid progenitors. However, we show PDCs arise exclusively from common myeloid progenitors (CMPs) characterized by low-level expression of several lymphoid-associated genes, including a RAG2/GFP reporter transgene. This conclusion is supported by both adoptive transfer experiments and an estrogen treatment strategy that led to marked depletion of very early lymphoid progenitors without affecting RAG2/GFP(+) CMPs or the developmental kinetics, RAG-mediated recombinase activity, and cytokine production of PDCs. These data suggest that PDCs arise exclusively from early myeloid progenitors and that promiscuous low-level expression of lymphoid-associated genes is a general feature of PDC progenitors among CMPs.
Harman
Benjamin C
BC
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 36th and Hamilton Walk, 230 John Morgan Bldg, Philadelphia, 19104, USA.
Miller
Juli P
JP
Nikbakht
Neda
N
Gerstein
Rachel
R
Allman
David
D
D019264:Adoptive Transfer; D000818:Animals; D019070:Cell Lineage; D004268:DNA-Binding Proteins / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology*; D004351:Drug Resistance; D004967:Estrogens / Q000494:pharmacology; D005434:Flow Cytometry; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D023461:Myeloid Progenitor Cells / Q000166:cytology*
D004268:DNA-Binding Proteins; D004967:Estrogens; C499308:Rag2 protein, mouse; D049452:Green Fluorescent Proteins