Role of gap junction in the expression of morphine-induced antinociception.
The present study was undertaken to investigate whether gap junctional communication could be involved in morphine-induced antinociceptive response using blockers of the gap junctional channel, carbenoxolone and Gap27. Intrathecal pretreatment with either carbenoxolone or Gap27 caused a dose-dependent attenuation of morphine-induced antinociception. Furthermore, the dose-response line for morphine-induced antinociception was shifted to the right by 2.53-fold following intrathecal treatment with carbenoxolone. These findings suggest that gap-junctional-dependent communication in the mouse spinal cord may play, at least in part, a role in the expression of morphine-induced antinociception.
Suzuki
Masami
M
Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Shinagawa-ku, Tokyo 142-8501, Japan.
Narita
Minoru
M
Nakamura
Atsushi
A
Suzuki
Tsutomu
T
D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002229:Carbenoxolone / Q000008:administration & dosage / Q000494:pharmacology; D017630:Connexins / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D017629:Gap Junctions / Q000502:physiology*; D007278:Injections, Spinal; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009020:Morphine / Q000008:administration & dosage / Q000494:pharmacology*; D009842:Oligopeptides; D010146:Pain / Q000517:prevention & control*; D010147:Pain Measurement / Q000379:methods
D000701:Analgesics, Opioid; D017630:Connexins; D009842:Oligopeptides; C455880:gap 27 peptide; D009020:Morphine; D002229:Carbenoxolone