Cloning, purification, crystallization and preliminary X-ray analysis of XC229, a conserved hypothetical protein from Xanthomonas campestris.
Xanthomonas campestris pv. campestris is a Gram-negative yellow-pigmented pathogenic bacterium that causes black rot, one of the major worldwide diseases of cruciferous crops. Its genome contains approximately 4500 genes, roughly one third of which have no known structure and/or function. However, some of these unknown genes are highly conserved among several different bacterial genuses. XC229 is one such protein containing 134 amino acids. It was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to a resolution of at least 1.80 A. It is cubic and belongs to space group I2(x)3, with unit-cell parameters a = b = c = 106.8 A. It contains one or two molecules per asymmetric unit.
Chin
Ko-Hsin
KH
Institute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan.
Kuo
Wei-Tien
WT
Chou
Chia-Cheng
CC
Shr
Hui-Lin
HL
Lyu
Ping-Chiang
PC
Wang
Andrew H-J
AH
Chou
Shan-Ho
SH
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D023281:Genomics; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D014961:X-Ray Diffraction; D016959:Xanthomonas campestris / Q000378:metabolism*
D001426:Bacterial Proteins; D011994:Recombinant Proteins