Long-term anergy in orally tolerized mice is linked to decreased B7.2 expression on B cells.
Durable antigen (Ag)-specific T- and B-cell anergy induced by oral tolerance is an attractive strategy for immunotherapy of allergic diseases. Here, we address the lasting effect of oral tolerance induction in naïve or primed mice to ovalbumin (OVA) on antibody production. Single feeding with OVA prior to immunization or double feeding, before and after Ag priming, in A/Sn mice, induced a long-lasting suppression of IgE, IgG1 and IgG2a responses up to 8 months after immunization. In contrast, primed-fed mice had transient IgE inhibition. Naive and double-treated mice showed marked Ag-specific unresponsiveness and scarce cytokines production. Inhibition of IL-2 and IFN-gamma secretion in naïve-fed mice were restored in the presence of anti-CD28 mAb plus Ag stimulation. The durable inhibition of Ab production in OVA-fed mice was related to the persistent decrease of B7.2 expression on B cells. Ag feeding in naive and primed status may be a prophylactic measure to avoid later Ag sensitization.
Futata
Eliana A
EA
Laboratório de Dermatologia e Imunodeficiências, Faculdade de Medicina da Universidade de São Paulo, Instituto de Medicina Tropical--Prédio II, Av Dr Enéas de Carvalho Aguiar, 500, 3 degrees Andar, 05403-000- São Paulo, Brazil.
de Brito
Cyro A
CA
Victor
Jefferson Russo
JR
Fusaro
Ana Elisa
AE
Oliveira
Célia R
CR
Maciel
Milton
M
da Silva Duarte
Alberto José
AJ
Sato
Maria Notomi
MN
D000284:Administration, Oral; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D051940:B7-2 Antigen / Q000096:biosynthesis / Q000235:genetics*; D060908:CTLA-4 Antigen; D017635:Clonal Anergy / Q000276:immunology*; D005260:Female; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis / Q000378:metabolism; D007700:Kinetics; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology*; D051381:Rats; D011920:Rats, Inbred WF
D015703:Antigens, CD; D051940:B7-2 Antigen; D060908:CTLA-4 Antigen; C556707:Ctla4 protein, mouse; D007132:Immunoglobulin Isotypes; D010047:Ovalbumin