title: Quantitative determination of paralytic shellfish poisoning toxins in shellfish using prechromatographic oxidation and liquid chromatography with fluorescence detection: collaborative study.
abstract: A collaborative study was conducted for the determination of paralytic shellfish poisoning (PSP) toxins in shellfish. The method used liquid chromatography with fluorescence detection after prechromatographic oxidation of the toxins with hydrogen peroxide and periodate. The PSP toxins studied were saxitoxin (STX), neosaxitoxin (NEO), gonyautoxins 2 and 3 (GTX2,3; together), gonyautoxins 1 and 4 (GTX1,4; together), decarbamoyl saxitoxin (dcSTX), B-1 (GTX5), C-1 and C-2 (C1,2; together), and C-3 and C-4 (C3,4; together). B-2 (GTX6) toxin was also included, but for qualitative identification only. Mussels, both blank and naturally contaminated, were mixed and homogenized to provide a variety of PSP toxin mixtures and concentration levels. The same procedure was followed with clams, oysters, and scallops. Twenty-one test samples in total were sent to 21 collaborators who agreed to participate in the study. Results were obtained from 18 laboratories representing 14 different countries. It is recommended that the method be adopted First Action by AOAC INTERNATIONAL.
authors.affiliation: Health Canada, Food Research Division, Bureau of Chemical Safety, Food Directorate, Banting Research Centre 2203D, Ottawa, Ontario, K1A 0L2, Canada. jimlawrence@sympatico.ca
authors.forename: James F
authors.initials: JF
authors.lastname: Lawrence
authors.forename: Barbara
authors.initials: B
authors.lastname: Niedzwiadek
authors.forename: Cathie
authors.initials: C
authors.lastname: Menard
mesh_terms: D000818:Animals; D001681:Biological Assay; D049872:Bivalvia; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002845:Chromatography / Q000379:methods*; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods*; D008387:Marine Toxins / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D010125:Ostreidae; D016877:Oxidants; D010100:Oxygen / Q000378:metabolism; D049897:Pectinidae; D015203:Reproducibility of Results; D012530:Saxitoxin / Q000031:analogs & derivatives / Q000032:analysis; D012758:Shellfish; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors; D014118:Toxins, Biological
chemical_list: D008387:Marine Toxins; D016877:Oxidants; D014118:Toxins, Biological; D012530:Saxitoxin; C034903:decarbamylsaxitoxin; C034902:neosaxitoxin; C013007:gonyautoxins; D010100:Oxygen