title: Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes.
abstract: We have developed spotted cell microarrays for measuring cellular phenotypes on a large scale. Collections of cells are printed, stained for subcellular features, then imaged via automated, high-throughput microscopy, allowing systematic phenotypic characterization. We used this technology to identify genes involved in the response of yeast to mating pheromone. Besides morphology assays, cell microarrays should be valuable for high-throughput in situ hybridization and immunoassays, enabling new classes of genetic assays based on cell imaging.
authors.affiliation: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, 2500 Speedway, University of Texas, Austin, TX 78712, USA.
authors.forename: Rammohan
authors.initials: R
authors.lastname: Narayanaswamy
authors.forename: Wei
authors.initials: W
authors.lastname: Niu
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Scouras
authors.forename: G Traver
authors.initials: GT
authors.lastname: Hart
authors.forename: Jonathan
authors.initials: J
authors.lastname: Davies
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
authors.forename: Vishwanath R
authors.initials: VR
authors.lastname: Iyer
authors.forename: Edward M
authors.initials: EM
authors.lastname: Marcotte
mesh_terms: D020869:Gene Expression Profiling; D005800:Genes, Fungal / Q000235:genetics; D016681:Genome, Fungal; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D010675:Pheromones / Q000235:genetics*; D051964:Receptors, Mating Factor / Q000235:genetics*; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D010675:Pheromones; D051964:Receptors, Mating Factor; D029701:Saccharomyces cerevisiae Proteins