title: [Screening of a phenanthrene-degrading bacterium and its degradation conditions].
abstract: Several PAHs-degrading bacteria were isolated from the soil near a petrochemicals factory, and one strain Fl0a identified as B. sphaericus was chosen for use. The study on the phenanthrene-degradation potential of the strain and its affecting factors showed that at 28 degrees C, the degradation rate of phenanthrene (50 mg x L(-1)) was 98.12% after 27 hours rotary culture, and 98.47% after 84 hours static culture. F10a had a good phenanthrene-degradation capability when the pH was 4, 6 and 8, but its growth was inhibited when pH was 10. Cr2+ was toxic to the strain, Cu2+ could delay the degradation of phenanthrene, while Zn2+ and Pb2+ had no significant effects. The degradation rate of phenanthrene (200 mg x L(-1)) was 99.6% after 84 hours rotary culture. A significant positive relationship was found between bacterial growth and phenanthrene degradation.
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environmnent, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China. linktozl@yahoo.com.cn
authors.forename: Le
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiafang
authors.initials: X
authors.lastname: Sheng
authors.forename: Shijin
authors.initials: S
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
mesh_terms: D001673:Biodegradation, Environmental; D003300:Copper / Q000737:chemistry; D006094:Gram-Positive Bacteria / Q000302:isolation & purification* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000737:chemistry; D010616:Phenanthrenes / Q000378:metabolism*; D012988:Soil Microbiology; D015032:Zinc / Q000737:chemistry
chemical_list: D010616:Phenanthrenes; D007854:Lead; C031181:phenanthrene; D003300:Copper; D015032:Zinc