title: Three types of spontaneously active anionic channels in malaria-infected human red blood cells.
abstract: The electrophysiological study of red blood cells (RBCs), using the patch-clamp technique, has been going through a renaissance with the recent discovery of novel channel activity in the host plasma membrane of Plasmodium falciparum-infected human RBCs (S.A. Desai et al., Nature 406, 1001-1005, 2000; S.M. Huber et al., EMBO J. 21 (2002) 22-30; S. Egee et al., J. Physiol. 542 (2002) 795-801). This arose from the finding that malaria-infected RBCs have altered permeability characteristics due to the induction of new permeation pathways (NPPs) (H. Ginsburg, Novartis Foundation Symposium 226 (1999) 99-108; K. Kirk, Physiol. Rev. 81 (2001) 495-537), which are defined, using non-electrophysiological techniques, as having the general characteristics of anion channels (i.e. high anion permeability, linear concentration dependence, inability to distinguish between stereo-isomers of permeant solutes). Discovering potent and specific inhibitors of the NPPs is an important therapeutic challenge, but too many questions remain unanswered: do the NPPs correspond to a single path or multiple pathways? Are they parasite-derived proteins? Are they up-regulated or modified endogenous quiescent red blood cell proteins? This article concerns the identification of different types of anionic channels that are expressed in malaria-infected human RBCs. Implications regarding the presence of these different types of channels in infected RBCs and their functional significance are discussed.
authors.affiliation: CNRS, UMR 7150, Station Biologique, Place G. Teissier, BP 74, 29682 Roscoff cedex, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Bouyer
authors.forename: Stéphane
authors.initials: S
authors.lastname: Egée
authors.forename: Serge L
authors.initials: SL
authors.lastname: Thomas
mesh_terms: D000818:Animals; D000838:Anions; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004594:Electrophysiology; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology* / Q000502:physiology; D006801:Humans; D007473:Ion Channels / Q000502:physiology*; D008288:Malaria / Q000097:blood* / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D010963:Plasmodium falciparum / Q000472:pathogenicity
chemical_list: D000838:Anions; D007473:Ion Channels