A highly sensitive and rapid assay for protein determination in human urine and penicillin using a Rayleigh light-scattering technique with benzeneazo-8-acetylamino-1-naphthol-3,6-disulfonic acid sodium salt.
A simple and sensitive method was conducted for the determination of trace amounts of proteins with benzeneazo-8-acetylamino-1-naphthol-3,6-disulfonic acid sodium salt (azophloxine, AP) using a Rayleigh light-scattering (RLS) technique. At pH 2.60 and in the presence of an emulsifier OP microemulsion, the RLS of AP can be greatly enhanced by proteins, owing to the interaction between AP and protein. The enhanced intensity is proportional to the concentration of proteins. Four proteins, including bovine serum albumin (BSA), human serum albumin (HSA), lysozyme (Lys) and gamma globulin (gamma-G) have been tested. For example, the linear range of BSA was 0 - 0.06 microg mL(-1) with detection limits of 2.38 ng mL(-1). The method was applied to the analysis of protein in human urine and penicillin samples with satisfactory results. The relative standard deviation was in all instances less than 4.0%, and the recovery was in the range of 97.5 - 104%.
Wei
Qin
Q
School of Chemistry & Chemical Engineering, Jinan University, Jinan 250022, China. sdjndxwq@263.net
Wu
Dan
D
Li
Yan
Y
Du
Bin
B
Wang
Jun Hong
JH
D001391:Azo Compounds / Q000737:chemistry*; D001547:Benzaldehydes / Q000737:chemistry; D002021:Buffers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D008823:Micelles; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry; D009282:Naphthalenesulfonates / Q000737:chemistry*; D010406:Penicillins / Q000652:urine*; D011506:Proteins / Q000032:analysis*; D011507:Proteinuria / Q000175:diagnosis* / Q000652:urine; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000737:chemistry; D016482:Urinalysis / Q000379:methods*; D005719:gamma-Globulins / Q000737:chemistry
D001391:Azo Compounds; D001547:Benzaldehydes; D002021:Buffers; D008823:Micelles; D009282:Naphthalenesulfonates; D010406:Penicillins; D011506:Proteins; D012709:Serum Albumin; D005719:gamma-Globulins; C037541:red 2G; D009113:Muramidase