title: Recombinant bovine uteroglobin at 1.6 A resolution: a preliminary X-ray crystallographic analysis.
abstract: Uteroglobin (UG) is a conserved protein which is induced by progesterone and secreted by the epithelia of various mammalian reproductive and respiratory organs. Recombinant bovine uteroglobin (recbUG), consisting of 80 amino acids with a C-terminal His6 tag, was overexpressed in Escherichia coli and purified. The protein was crystallized in two geometric forms, rhomboid and cuneate (wedge-shaped), by the hanging-drop vapour-diffusion method at 295 K. The rhomboid crystals diffracted to a maximum resolution of 1.6 A using synchrotron radiation. These crystals belong to space group P2(1)2(1)2, with unit-cell parameters a = 81.42, b = 82.82, c = 45.26 A, and contain four monomers per asymmetric unit. The cuneate crystals diffracted to 2.35 A resolution using a rotating-anode generator. These crystals belong to space group C222(1), with unit-cell parameters a = 43.39, b = 93.94, c = 77.30 A, and contain two molecules per asymmetric unit.
authors.affiliation: Institute of Anatomy and Reproductive Biology, Medical School RWTH Aachen, Wendlingweg 2, 52074 Aachen, Germany.
authors.forename: Victoria
authors.initials: V
authors.lastname: von der Decken
authors.forename: Heinrich
authors.initials: H
authors.lastname: Delbrück
authors.forename: Andreas
authors.initials: A
authors.lastname: Herrler
authors.forename: Henning M
authors.initials: HM
authors.lastname: Beier
authors.forename: Rainer
authors.initials: R
authors.lastname: Fischer
authors.forename: Kurt M V
authors.initials: KM
authors.lastname: Hoffmann
mesh_terms: D000818:Animals; D002417:Cattle; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014598:Uteroglobin / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D014598:Uteroglobin