title: Capillary zone electrophoretic determination of phenolic compounds in chess (Bromus inermis L.) plant extracts.
abstract: A simple CZE method for quantification of phenolic compounds (vanillin, cinnamic, sinapic, chlorogenic, syringic, ferulic, benzoic, p-coumaric, vanillic, p-hydroxybenzoic, rosmarinic, caffeic, gallic and protocatechuic acids) in less than 10 min using 20 mM sodium tetraborate (pH 9.2) with 5% v/v methanol as a BGE and with UV detection at 254 nm is described. The LODs (3 S/N) ranged between 0.02 and 0.12 microg/ mL. Repeatabilities (RSDs) were 0.66-1.8 and 1.56-4.23% for migration times and peak areas (n = 5), respectively. The method was applied to the determination of phenolic compounds in chess (Bromus inermis L.) after Soxhlet extraction and purification of the crude extracts with SPE procedures. The results compared well with those obtained by liquid chromatographic method. B. inermis was found as a suitable model plant containing a broad spectrum of phenolic compounds in easily detectable concentrations and as a potential source of antioxidants.
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry Brno, Czech Republic.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Sterbová
authors.forename: Jirí
authors.initials: J
authors.lastname: Vlcek
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Kubán
mesh_terms: D031724:Bromus / Q000737:chemistry*; D002021:Buffers; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D002021:Buffers; D004573:Electrolytes; D010636:Phenols; D010936:Plant Extracts