Transcranial magnetic stimulation attenuates cell loss and oxidative damage in the striatum induced in the 3-nitropropionic model of Huntington's disease.
An investigation was conducted on the effect of transcranial magnetic field stimulation (TMS) on the free radical production and neuronal cell loss produced by 3-nitropropionic acid in rats. The effects of 3-nitropropionic acid were evaluated by examining the following changes in: the quantity of hydroperoxides and total radical-trapping antioxidant potential (TRAP), lipid peroxidation products, protein carbonyl groups, reduced glutathione (GSH) content, glutathione peroxidase (GSH-Px), catalase and succinate dehydrogenase (SDH) activities; total nitrite and cell death [morphological changes, quantification of neuronal loss and lactate dehydrogenase (LDH) levels]. Our results reveal that 3-nitropropionic acid induces oxidative and nitrosative stress in the striatum, prompts cell loss and also shows that TMS prevents the harmful effects induced by the acid. In conclusion, the results show the ability of TMS to modify neuronal response to 3-nitropropionic acid.
Túnez
Isaac
I
Departamento de Bioquímica y Biología Molecular, y, Facultad de Medicina, Universidad de Córdoba, Córdoba, Spain. fm2tufii@uco.es
Drucker-Colín
René
R
Jimena
Ignacio
I
Medina
Francisco J
FJ
Muñoz
Maria del Carmen
Mdel C
Peña
José
J
Montilla
Pedro
P
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D016923:Cell Death / Q000528:radiation effects*; D003342:Corpus Striatum / Q000473:pathology* / Q000528:radiation effects; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D006816:Huntington Disease / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D006861:Hydrogen Peroxide / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D056913:Mediator Complex Subunit 1; D009573:Nitrites / Q000378:metabolism; D009574:Nitro Compounds; D018384:Oxidative Stress / Q000528:radiation effects*; D011422:Propionates; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D013385:Succinate Dehydrogenase / Q000378:metabolism; D050781:Transcranial Magnetic Stimulation; D014157:Transcription Factors / Q000378:metabolism
C538857:Med1 protein, rat; D056913:Mediator Complex Subunit 1; D009573:Nitrites; D009574:Nitro Compounds; D011422:Propionates; D014157:Transcription Factors; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013385:Succinate Dehydrogenase; D005978:Glutathione; C015392:3-nitropropionic acid