title: Expression, purification and X-ray crystallographic analysis of thioredoxin from Streptomyces coelicolor.
abstract: Thioredoxins are ubiquitous proteins that serve as reducing agents and general protein disulfide reductases. In turn, they are reduced by electrons obtained from the NADPH-containing thioredoxin reductase. Thioredoxins have been isolated and characterized from a large number of organisms. The Gram-positive bacterium Streptomyces coelicolor contains three thioredoxins that are involved in unknown biological processes. trxA from S. coelicolor was cloned and expressed in Escherichia coli and the protein purified and crystallized using the hanging-drop method of vapour diffusion. The crystal structure of thioredoxin A has been determined at 1.5 A resolution using a synchrotron-radiation source. The protein reveals a thioredoxin-like fold with a typical CXXC active site. The crystal exhibits the symmetry of space group P2(1)2(1)2, with unit-cell parameters a = 43.6, b = 71.8, c = 33.2 A.
authors.affiliation: Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynska dolina CH-1, 842 15 Bratislava, Slovak Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Stefankova
authors.forename: Jana
authors.initials: J
authors.lastname: Maderova
authors.forename: Imrich
authors.initials: I
authors.lastname: Barak
authors.forename: Marta
authors.initials: M
authors.lastname: Kollarova
authors.forename: Zbyszek
authors.initials: Z
authors.lastname: Otwinowski
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D017931:DNA Primers; D004926:Escherichia coli / Q000737:chemistry; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D048372:Streptomyces coelicolor / Q000737:chemistry*; D017356:Synchrotrons; D013879:Thioredoxins / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D013879:Thioredoxins