Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci.
We developed a new multiplex PCR assay for detection of Panton-Valentine leukocidin virulence genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. This assay is simple, rapid, and accurate and offers the potential for prompt detection of newly emerging community-associated methicillin-resistant Staphylococcus aureus.
McClure
Jo-Ann
JA
Centre for Antimicrobial Resistance, Calgary Health Region/Calgary Laboratory Services/University of Calgary, Calgary, Alberta, Canada.
Conly
John M
JM
Lau
Vicky
V
Elsayed
Sameer
S
Louie
Thomas
T
Hutchins
Wendy
W
Zhang
Kunyan
K
D000416:Alberta / Q000453:epidemiology; D001427:Bacterial Toxins / Q000235:genetics*; D001431:Bacteriological Techniques; D001483:Base Sequence; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D005098:Exotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D007956:Leukocidins; D016106:Methicillin Resistance / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin