Transcriptome-scale similarities between mouse and human skeletal muscles with normal and myopathic phenotypes.
BACKGROUND
Mouse and human skeletal muscle transcriptome profiles vary by muscle type, raising the question of which mouse muscle groups have the greatest molecular similarities to human skeletal muscle.


METHODS
Orthologous (whole, sub-) transcriptome profiles were compared among four mouse-human transcriptome datasets: (M) six muscle groups obtained from three mouse strains (wildtype, mdx, mdx5cv); (H1) biopsied human quadriceps from controls and Duchenne muscular dystrophy patients; (H2) four different control human muscle types obtained at autopsy; and (H3) 12 different control human tissues (ten non-muscle).


RESULTS
Of the six mouse muscles examined, mouse soleus bore the greatest molecular similarities to human skeletal muscles, independent of the latters' anatomic location/muscle type, disease state, age and sampling method (autopsy versus biopsy). Significant similarity to any one mouse muscle group was not observed for non-muscle human tissues (dataset H3), indicating this finding to be muscle specific.


CONCLUSIONS
This observation may be partly explained by the higher type I fiber content of soleus relative to the other mouse muscles sampled.
Kho
Alvin T
AT
Children's Hospital Informatics Program, Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts, USA. alvin_kho@hms.harvard.edu
Kang
Peter B
PB
Kohane
Isaac S
IS
Kunkel
Louis M
LM
D000818:Animals; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009135:Muscular Diseases / Q000235:genetics* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D014158:Transcription, Genetic