Chlamydia pneumoniae infections augment atherosclerotic lesion formation: a role for serum amyloid P.
Multiple reports have demonstrated an association between Chlamydia pneumoniae (Cpn) and cardiovascular disease. In this study we evaluated the effect of Cpn infections on early lesion progression in C57BL/6J mice. Since plaque formation in these mice does not develop past the initial stage, we thought these mice might be a better model for unravelling the effect of Cpn infection on early lesion type progression. C57BL/6J mice were fed an atherogenic diet and injected 10 times with 5 x 10(7) IFU Cpn or mock. At sacrifice, lesion number, size and type were analysed. To study the role of Cpn in inflammation, serum amyloid P (SAP) in plasma was determined as well as T-cells, macrophages and SAP in the lesions. In the aortic sinus of both groups, type 2 lesions were found. Cpn infection resulted in a 2.2-fold increase in total lesion size (Cpn: 10821+/-2429 microm(2)vs mock: 5022+/-1348 microm(2); p=0.04). No difference in lesion number was observed. Also, Cpn infection increased SAP in the lesions from 1.10(-4)+/-0.1.10(-4) SAP-positive cells/lesion area to 10.10(-4)+/-1.10(-4) SAP-positive cells/lesion area (p=0.05). The influx of T-lymphocytes and macrophages in the lesions as well as SAP plasma levels were not different between groups. Multiple Cpn infections resulted in a significant increase in total lesion size of C57BL/6J mice. Increase in total SAP-positive area in infected mice suggests a role for this acute-phase protein in lesion enlargement.
Ezzahiri
R
R
Department of Surgery, University Hospital Maastricht, The Netherlands.
Stassen
F R M
FR
Kurvers
H R M
HR
Dolmans
V
V
Kitslaar
P J E H M
PJ
Bruggeman
C A
CA
D000818:Animals; D050197:Atherosclerosis / Q000097:blood / Q000382:microbiology* / Q000473:pathology; D023521:Chlamydophila Infections / Q000097:blood / Q000150:complications* / Q000382:microbiology / Q000473:pathology; D016993:Chlamydophila pneumoniae / Q000254:growth & development*; D002784:Cholesterol / Q000097:blood; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006651:Histocytochemistry; D008264:Macrophages / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D000683:Serum Amyloid P-Component / Q000378:metabolism*; D012850:Sinus of Valsalva / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000473:pathology; D014280:Triglycerides / Q000097:blood
D004269:DNA, Bacterial; D000683:Serum Amyloid P-Component; D014280:Triglycerides; D002784:Cholesterol