[Retroviral-mediated gene transfer as an effective tool for the in vitro genetic transformation of chicken embryonic cells and production of transgenic chickens].
The data on the in vitro and in vivo (into embryonic disk) retroviral-mediated transfer of genetic information into chicken embryonic cells are presented. The estimated transformation frequency of the cultured target cells constituted 8 x 10(-4) to 5 x 10(-3). A transgenic rooster, carrying recombinant DNA in blood, heart, liver, and intestine cells, was obtained.
Volkova
N A
NA
Zinov'eva
N A
NA
Volkova
L V
LV
Ernst
L K
LK
D000818:Animals; D030801:Animals, Genetically Modified; D002478:Cells, Cultured; D002642:Chick Embryo / Q000166:cytology*; D002645:Chickens / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D066298:In Vitro Techniques; D009928:Organ Specificity; D010957:Plasmids; D012190:Retroviridae