title: Acceleration of de novo bone formation following application of autogenous bone to particulated anorganic bovine material in vivo.
abstract: OBJECTIVE
  This prospective animal study examined the de novo bone formation following application of deproteinized bovine bone matrix (DBBM) with or without autogenous bone (AB) to osseous defects.
  
  
  METHODS
  Defects of defined size were created in the frontal skull of domestic pigs and filled with DBBM alone (group A) and DBBM+25%AB (group B). De novo bone formation was analyzed qualitatively and quantitatively at 9 different times (0.5, 1, 2, 3, 4, 6, 8, 12, 26 weeks) by means of light microscopy, microradiography, and statistical analysis.
  
  
  RESULTS
  Histological analysis indicated sufficient osseointegration of DBBM in both groups. Microradiography demonstrated a significant increase of bone formation in group B after 6 weeks (P = .0159) and 8 weeks (P = .0317).
  
  
  CONCLUSIONS
  The addition of 25%AB to DBBM results in accelerated de novo bone formation in osseous defects. This effect is likely caused by osteoinductive properties of cellular elements transplanted with the autogenous bone.
authors.affiliation: Department of Oral and Maxillofacial Surgery/Plastic Surgery, University of Jena, Jena, Germany. Michael.Thorwarth@med.uni-jena.de
authors.forename: Michael
authors.initials: M
authors.lastname: Thorwarth
authors.forename: Karl Andreas
authors.initials: KA
authors.lastname: Schlegel
authors.forename: Falk
authors.initials: F
authors.lastname: Wehrhan
authors.forename: Safwan
authors.initials: S
authors.lastname: Srour
authors.forename: Stefan
authors.initials: S
authors.lastname: Schultze-Mosgau
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000637:transplantation*; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology*; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D005260:Female; D008852:Microradiography; D008903:Minerals / Q000494:pharmacology*; D011446:Prospective Studies; D012886:Skull / Q000601:surgery; D018709:Statistics, Nonparametric; D034421:Sus scrofa; D013552:Swine; D014182:Transplantation, Autologous
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D008903:Minerals