[Determination of opiates in biological human samples by liquid chromatography-tandem mass spectrometry].
OBJECTIVE
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of opiates in biological samples according to the emerging problem in drugs abuse.


METHODS
Opiates such as heroin, 6-acetylmorphine, morphine, codeine, acetylcodeine, hydrocodone and hydromorphone were isolated from human blood, urine, oral fluid and hair using a simple extraction and consequently analyzed using LC-MS/MS. The method was evaluated by real cases.


RESULTS
The mobile phase give the optimum separation for opiates. The detection limit of morphine in urine with dilution and liquid-liquid extraction and in hair is 10ng/mL, 0.01 ng/mL and 0.01 ng/mg, respectively.


CONCLUSIONS
The method is simple and rapid, offering superior sensitivity and selectivity for opiates. The target compounds comprising hydrocodone and hydromorphone enlarge the applied area.
Xiang
Ping
P
Institute of Forensic Sciences, Ministry of Justice, P.R.China.
Shen
Min
M
Shen
Bao-hua
BH
Ma
Dong
D
Bu
Jun
J
Jiang
Yan
Y
Zhuo
Xian-yi
XY
D002853:Chromatography, Liquid; D003061:Codeine / Q000032:analysis; D005554:Forensic Medicine / Q000379:methods; D006197:Hair / Q000737:chemistry*; D006801:Humans; D006853:Hydrocodone / Q000032:analysis; D004091:Hydromorphone / Q000032:analysis; D009020:Morphine / Q000032:analysis; D009294:Narcotics / Q000032:analysis*; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry
D009294:Narcotics; D006853:Hydrocodone; D009020:Morphine; D004091:Hydromorphone; D003061:Codeine