Recognition imaging with a DNA aptamer.
We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (approximately 90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.
Lin
Liyun
L
Department of Physics, Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Wang
Hongda
H
Liu
Yan
Y
Yan
Hao
H
Lindsay
Stuart
S
D000538:Aluminum Silicates / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006801:Humans; D007073:Immunoglobulin E / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D013917:Thrombin / Q000737:chemistry
D000538:Aluminum Silicates; D052157:Aptamers, Nucleotide; D007073:Immunoglobulin E; D004247:DNA; D013917:Thrombin; C011934:mica