Effects of the frequency and duration of cyclic stress on the mechanical properties of cultured collagen fascicles from the rabbit patellar tendon.
The effects of frequency or duration of cyclic stress on the mechanical properties of collagen fascicles were studied by means of in vitro tissue culture experiments. Collagen fascicles of approximately 300 microm in diameter were obtained from rabbit patellar tendons. During culture, cyclic stress having the peak stress of approximately 2 MPa was applied to the fascicles at 1 Hz for 1 hour/day (1 Hz-1 h group), at 1 Hz for 4 hours/day (1 Hz-4 h group), or at 4 Hz for 1 hour/day (4 Hz-1 h group). The frequency of 4 Hz and the duration of 1 hour/day are considered to be similar to those of the in vivo stress applied to fascicles in the intact rabbit patellar tendon. After culture for 1 or 2 weeks, the mechanical properties of the fascicles were determined using a microtensile tester, and were compared to the properties of non-cultured, fresh fascicles (control group) and the fascicles cultured under no load condition (non-loaded group). The tangent modulus and tensile strength of fascicles in the 4 Hz-1 h group were similar to those in the control group; however, the fascicles of the 1 Hz-1 h and 1 Hz-4 h groups had significantly lower values than those of the control group. There was no significant difference in the tensile strength between the 1 Hz-1 h and non-loaded groups, although the strength in the 1 Hz-4 h group was significantly higher than that of the non-loaded group. It was concluded that the frequency and duration of cyclic stress significantly affect the mechanical properties of cultured collagen fascicles. If we apply cyclic stress having the frequency and duration which are experienced in vivo, the biomechanical properties are maintained at control, normal level. Lower frequencies or less cycles of applied force induce adverse effects.
Yamamoto
Ei
E
Biomechanics Laboratory, Department of Mechanical Engineering and Biomimetics, School of Biology-Oriented Science and Technology, Kinki University, Naga, Wakayama 649-6493, Japan. ei@waka.kindai.ac.jp
Kogawa
Daisuke
D
Tokura
Susumu
S
Hayashi
Kozaburo
K
D000818:Animals; D002478:Cells, Cultured; D019245:Compressive Strength / Q000502:physiology; D004548:Elasticity; D005260:Female; D024022:Fibrillar Collagens / Q000502:physiology*; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D009991:Oscillometry / Q000379:methods; D010329:Patella / Q000502:physiology*; D017847:Patellar Ligament / Q000502:physiology*; D010507:Periodicity; D010812:Physical Stimulation / Q000379:methods*; D011817:Rabbits; D013314:Stress, Mechanical; D013710:Tendons / Q000502:physiology*; D013718:Tensile Strength / Q000502:physiology
D024022:Fibrillar Collagens