Oxidative DNA cleavage induced by an iron(III) flavonoid complex: synthesis, crystal structure and characterization of chlorobis(flavonolato)(methanol) iron(III) complex.
A flavonol iron(III) complex, [Fe(flavonolato)(2)Cl(MeOH)], has been prepared. The compound has been characterized by X-ray crystallography, spectroscopy, magnetism and electronic paramagnetic resonance (EPR) at X- and Q-band. The geometrical environment around the metal is best described as rhombic distorted octahedral. This distortion has also been inferred from the magnetic measurements and from the EPR spectra at different temperatures, E/D(rhombicity parameter) approximately 0.06. The DNA cleavage activity of the iron(III) complex with and without ascorbate/hydrogen peroxide is reported. Mechanisms of the oxidative cleavage have been proposed when DNA strand scission is performed both with and without ascorbate/hydrogen peroxide activation.
El Amrani
Fátima Ben Allal
FB
Departament de Química Inorgànica, Universitat de València, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain.
Perelló
Lourdes
L
Real
Jose A
JA
González-Alvarez
Marta
M
Alzuet
Gloria
G
Borrás
Joaquín
J
García-Granda
Santiago
S
Montejo-Bernardo
José
J
D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004578:Electron Spin Resonance Spectroscopy; D005290:Ferric Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D008958:Models, Molecular; D015394:Molecular Structure; D018384:Oxidative Stress; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D005290:Ferric Compounds; C512472:chlorobis(flavonolato)(methanol) iron(III); D004247:DNA