title: A comprehensive expression analysis of the WRKY gene superfamily in rice plants during defense response.
abstract: To understand the transcriptional regulatory mechanism of host genes during the activation of defense responses in rice, we isolated WRKY transcription factors whose expressions were altered upon attack of the fungal pathogen Magnaporthe grisea, the causal agent of the devastating rice blast disease. A systematic expression analysis of OsWRKYs (Oryza sativa L. WRKYs) revealed that among 45 tested genes the expression of 15 genes was increased remarkably in an incompatible interaction between rice and M. grisea. Twelve of the M. grisea-inducible OsWRKY genes were also differentially regulated in rice plants infected with the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). In experiments with defense signaling molecules, the expression of two genes, OsWRKY45 and OsWRKY62, was increased in salicylic acid (SA)-treated leaves and the expression of three genes, OsWRKY10, OsWRKY82, and OsWRKY85 was increased by jasmonic acid (JA) treatment. OsWRKY30 and OsWRKY83 responded to both SA- and JA treatments. The expression profiles suggest that a large number of WRKY DNA-binding proteins are involved in the transcriptional activation of defense-related genes in response to rice pathogens.
authors.affiliation: Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University, Yongin, 449-701, Korea.
authors.forename: Hak-Seung
authors.initials: HS
authors.lastname: Ryu
authors.forename: Muho
authors.initials: M
authors.lastname: Han
authors.forename: Sang-Kyu
authors.initials: SK
authors.lastname: Lee
authors.forename: Jung-Il
authors.initials: JI
authors.lastname: Cho
authors.forename: Nayeon
authors.initials: N
authors.lastname: Ryoo
authors.forename: Sunggi
authors.initials: S
authors.lastname: Heu
authors.forename: Youn-Hyung
authors.initials: YH
authors.lastname: Lee
authors.forename: Seong Hee
authors.initials: SH
authors.lastname: Bhoo
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Wang
authors.forename: Tae-Ryong
authors.initials: TR
authors.lastname: Hahn
authors.forename: Jong-Seong
authors.initials: JS
authors.lastname: Jeon
mesh_terms: D015152:Blotting, Northern; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D006790:Host-Parasite Interactions; D020082:Magnaporthe / Q000502:physiology; D005810:Multigene Family / Q000235:genetics*; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology; D054883:Oxylipins; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D014974:Xanthomonas / Q000502:physiology
chemical_list: D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D012333:RNA, Messenger; C011006:jasmonic acid; D020156:Salicylic Acid