title: Amino acid sequence of RTX-A's isoform actinoporin from the sea anemone, Radianthus macrodactylus.
abstract: The amino acid sequence of actinoporin RTX-A (175 aa) from the sea anemone Radianthus macrodactylus was determined by sequencing of clones obtained via amplification of cDNA. It was established that RTX-A possessed high homology with HmgIII from Heteractis magnifica (87%) and StI, StII from Stichodactyla helianthus (84 and 87%, respectively). The analysis of structural and functional relationships within RTX-A was carried out. The some disagreement concerning to significant role of several amino acid residues for actinoporins exhibition of hemolytic activity was found.
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far East Branch, Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russian Federation. a.ilyina@gmail.com
authors.forename: Anna
authors.initials: A
authors.lastname: Il'ina
authors.forename: Aleksey
authors.initials: A
authors.lastname: Lipkin
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Barsova
authors.forename: Marina
authors.initials: M
authors.lastname: Issaeva
authors.forename: Elena
authors.initials: E
authors.lastname: Leychenko
authors.forename: Konstantin
authors.initials: K
authors.lastname: Guzev
authors.forename: Margarita
authors.initials: M
authors.lastname: Monastyrnaya
authors.forename: Sergey
authors.initials: S
authors.lastname: Lukyanov
authors.forename: Emma
authors.initials: E
authors.lastname: Kozlovskaya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003064:Cnidarian Venoms / Q000737:chemistry*; D003603:Cytotoxins / Q000737:chemistry*; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry; D012615:Sea Anemones / Q000737:chemistry*; D016415:Sequence Alignment
chemical_list: D003064:Cnidarian Venoms; D003603:Cytotoxins; D020033:Protein Isoforms