[Repair of mandibular central fissures in rabbits with hBMP-2 gene modified tissue engineered bone].
OBJECTIVE
To evaluate the effectiveness of the tissue engineered bone substitute loaded with periosteal-derived osteoblasts (POBs) transfected by adenovirus mediated human bone morphogenetic protein-2 gene (Ad-hBMP-2) in the repair of rabbit mandibular central fissures.


METHODS
45 rabbits with the soft tissue in the mandibular central fissures removed were randomly divided into 5 groups, group I: Ad-hBMP-2 transfected POBs/bioglass group (n=10); group II: adenovirus mediated enhanced green fluorescent protein (Ad-EGFP) gene transfected POBs/bioglass group (n=10); group III: untransfected POBs/bioglass group (n=10); group IV: single bioglass group (n=10); group V: control group (n=5). The above bone substitutes were implanted in the rabbit mandibular central fissures respectively except group V. The samples were studied by gross, X-ray, histomorphology, histomorphometrical analysis and biomechanics after 2, 4, 8, 12 weeks respectively. One-way ANOVA was used for statistical analysis.


RESULTS
In gross view, the rabbit mandibular central fissures in group I were replaced by new bone including cortical bone from the 4th week. X-ray examination showed that the higher bone density was found in the rabbit mandibular central fissures of group I 4 to 8 weeks after implantation. Histomorphometrical analysis showed much more new bony callus in group I than in other groups (P<0.01). The maximal anti-bending load and bending rigidity of the implanted bone substitute of group I were significantly higher than those of group II, III and IV (P<0.01).


CONCLUSIONS
The tissue engineered bone substitute loaded with POBs transfected by human BMP-2 gene could get the best result in the repair of rabbit mandibular central fissures, therefore, it is likely to be used in the repair of alveolar clefts.
Ye
Zhan-chao
ZC
Stomatology College, Shandong University, Jinan 250012, Shangdong Province, China.
Wei
Feng-cai
FC
Wang
Ke-tao
KT
Sun
Shan-zhen
SZ
Zhao
Hua-qiang
HQ
Li
Guo-ju
GJ
D000256:Adenoviridae; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics*; D018786:Bone Substitutes; D002516:Ceramics; D003750:Dental Fissures / Q000628:therapy*; D006801:Humans; D008334:Mandible; D010006:Osteoblasts; D011817:Rabbits; D011897:Random Allocation; D023822:Tissue Engineering; D014162:Transfection; D014945:Wound Healing
C022107:Bioglass; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes