[Some markers of oxidative stress in hypertension induced by chronic inhibition of nitric oxide synthase].
The aim of this study was to estimate some markers of oxidative stress in plasma and kidney of rats with experimental hypertension induced by chronic inhibition of nitric oxide synthase (NOS) by L-NAME (methyl ester of N-nitro-L-arginin). We investigated the concentration of lipid peroxidation products such as aldehydes, lipid hydroperoxides and the total antioxidant status (FRAP) in plasma. We also measured the concentration of reduced SH groups in plasma, renal cortex and medulla. The chronic blockade of NO synthesis resulted in elevation of systolic blood pressure which was associated with increase (29.2%) in SH groups concentration in renal cortex. The level of lipid peroxidation products and FRAP in plasma and renal medulla remained unchanged. We conclude that in experimental hypertension induced by chronic inhibition of NOS there are no changes in plasma markers of oxidative stress; whereas in renal cortex we noted significant increase in reduced SH groups. The alteration of thiols synthesis may contribute in the development of pathogenic changes in renal structure and function, especially in NO activity.
Wójcicka
Grazyna
G
Katedra i Zakład Patofizjologii Akademii Medycznej w Lublinie.
Jamroz
Anna
A
Bełtowski
Jerzy
J
D000302:Adrenal Cortex / Q000378:metabolism / Q000503:physiopathology; D000447:Aldehydes / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers; D004791:Enzyme Inhibitors; D006973:Hypertension / Q000201:enzymology / Q000209:etiology / Q000503:physiopathology; D015227:Lipid Peroxidation; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
D000447:Aldehydes; D000975:Antioxidants; D015415:Biomarkers; D004791:Enzyme Inhibitors; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester