title: Crystallization and preliminary X-ray crystallographic study of disproportionating enzyme from potato.
abstract: Disproportionating enzyme (D-enzyme; EC 2.4.1.25) is a 59 kDa protein that belongs to the alpha-amylase family. D-enzyme catalyses intramolecular and intermolecular transglycosylation reactions of alpha-1,4 glucan. A crystal of the D-enzyme from potato was obtained by the hanging-drop vapour-diffusion method. Preliminary X-ray data showed that the crystal diffracts to 2.0 A resolution and belongs to space group C222(1), with unit-cell parameters a = 69.7, b = 120.3, c = 174.2 A.
authors.affiliation: Laboratory of Enzyme Chemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Imamura
authors.forename: Takanori
authors.initials: T
authors.lastname: Matsuura
authors.forename: Zhengmao
authors.initials: Z
authors.lastname: Ye
authors.forename: Takeshi
authors.initials: T
authors.lastname: Takaha
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Fujii
authors.forename: Masami
authors.initials: M
authors.lastname: Kusunoki
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Nitta
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D006004:Glycogen Debranching Enzyme System / Q000737:chemistry* / Q000302:isolation & purification; D006031:Glycosylation; D010940:Plant Proteins / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D017931:DNA Primers; D006004:Glycogen Debranching Enzyme System; D010940:Plant Proteins; D011994:Recombinant Proteins; C020569:4 alpha-glucanotransferase