title: Unique microRNA molecular profiles in lung cancer diagnosis and prognosis.
abstract: MicroRNA (miRNA) expression profiles for lung cancers were examined to investigate miRNA's involvement in lung carcinogenesis. miRNA microarray analysis identified statistical unique profiles, which could discriminate lung cancers from noncancerous lung tissues as well as molecular signatures that differ in tumor histology. miRNA expression profiles correlated with survival of lung adenocarcinomas, including those classified as disease stage I. High hsa-mir-155 and low hsa-let-7a-2 expression correlated with poor survival by univariate analysis as well as multivariate analysis for hsa-mir-155. The miRNA expression signature on outcome was confirmed by real-time RT-PCR analysis of precursor miRNAs and cross-validated with an independent set of adenocarcinomas. These results indicate that miRNA expression profiles are diagnostic and prognostic markers of lung cancer.
authors.affiliation: Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Nozomu
authors.initials: N
authors.lastname: Yanaihara
authors.forename: Natasha
authors.initials: N
authors.lastname: Caplen
authors.forename: Elise
authors.initials: E
authors.lastname: Bowman
authors.forename: Masahiro
authors.initials: M
authors.lastname: Seike
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kumamoto
authors.forename: Ming
authors.initials: M
authors.lastname: Yi
authors.forename: Robert M
authors.initials: RM
authors.lastname: Stephens
authors.forename: Aikou
authors.initials: A
authors.lastname: Okamoto
authors.forename: Jun
authors.initials: J
authors.lastname: Yokota
authors.forename: Tadao
authors.initials: T
authors.lastname: Tanaka
authors.forename: George Adrian
authors.initials: GA
authors.lastname: Calin
authors.forename: Chang-Gong
authors.initials: CG
authors.lastname: Liu
authors.forename: Carlo M
authors.initials: CM
authors.lastname: Croce
authors.forename: Curtis C
authors.initials: CC
authors.lastname: Harris
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs