[Azoospermia factor microdeletions in idiopathic azoospermia and severe oligozoospermia].
OBJECTIVE
To observe the relationship between microdeletions of AZF( azoospermia factor) on Y chromosome in male with idiopathic azoospermia and severe oligozoospermia.


METHODS
Only patients with an apparently normal 46,XY karyotype and normal FSH, LH and T were included in this study. Multiplex PCR was used to detect the sequence-tagged sites( STS) as follows :sY84, sY86, sY127, sY134, sY152, sY153, sY254, sY255, and ZFX/Y was used as internal control gene.


RESULTS
No microdeletion was detected in the control whereas 8 microdeletion cases existed in 67 idiopathic azoospermia and severe oligozoospermia, including 4 in AZFc, 2 in AZFa + AZFc, 1 in AZFc + AZFb, and 1 in AZFb. The prevalence rate of microdeletion was 11.94%, which was statistically different from the control.


CONCLUSIONS
Microdeletions in the AZF regions on the long arm of the Y-chromosome are associated with idiopathic azoospermic and severely oligozoospermic men. Multiplex PCR was a rapid and reliable method for screening microdeletions of AZF.
Jia
Yi-Fang
YF
Key Laboratory for Improving Birth Outcome Technique of Shandong Provincial Institute of Family Planning Resaerch, Jinan, Shandong 250002, China. jyf690629@163.com
Wu
Ai-Hua
AH
Qiu
Yi
Y
Qu
Ji-Ying
JY
Song
Chang-Zheng
CZ
D000328:Adult; D053713:Azoospermia / Q000235:genetics*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y; D056426:Genetic Loci; D006801:Humans; D007621:Karyotyping; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D016133:Polymerase Chain Reaction; D029589:Seminal Plasma Proteins / Q000235:genetics*
D029589:Seminal Plasma Proteins