Detection of bile salt-dependent lipase, a 110 kDa pancreatic protein, in urines of healthy subjects.
Bile salt-dependent lipase (BSDL), a 110 kDa glycoprotein secreted by the pancreatic acinar cells, participates in the duodenal hydrolysis of dietary lipid esters. Recent in vitro and in vivo studies demonstrated that the BSDL reaches the blood via a transcytosis motion through enterocytes, suggesting that this enzyme may play a role in vascular biology. Once in the blood, BSDL should be eliminated. We address the hypothesis that BSDL may be filtered by the glomerulus and eliminated in urines. Immunological methods and proteomic were used to detect and to characterize BSDL in urine. The immunoreactive form of BSDL was detected in urines of 36 male subjects devoid of renal failure. Proteomic demonstrated that the immunoreactive protein is BSDL. Experiments using a monoclonal antibody to the oncofetal glycoform of pancreatic BSDL suggested that the protein is not expressed by renal cells but originates from the pancreas via circulation. We demonstrate that under normal physiological conditions, BSDL, a high-molecular weight blood glycoprotein, can be filtered by the renal glomerulus to be eliminated in urines.
Comte
B
B
INSERM U-559 and Faculté de Médecine, Univ de la Méditerranée, Marseille, France.
Franceschi
C
C
Sadoulet
M-O
MO
Silvy
F
F
Lafitte
D
D
Benkoel
L
L
Nganga
A
A
Daniel
L
L
Bernard
J-P
JP
Lombardo
D
D
Mas
E
E
D000328:Adult; D000595:Amino Acid Sequence; D001692:Biological Transport / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D005374:Filtration; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D007678:Kidney Glomerulus / Q000098:blood supply / Q000502:physiology*; D007685:Kidney Tubules, Collecting / Q000737:chemistry / Q000502:physiology; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D008969:Molecular Sequence Data; D010179:Pancreas / Q000737:chemistry* / Q000502:physiology; D011485:Protein Binding; D040901:Proteomics; D002787:Sterol Esterase / Q000032:analysis / Q000378:metabolism* / Q000652:urine*
C054784:bile salt-stimulated lipase; D002787:Sterol Esterase