Taenia solium and Taenia ovis: stage-specific expression of the vaccine antigen genes, TSOL18, TSOL16, and homologues, in oncospheres.
Recombinant antigens that have been cloned from Taenia solium and Taenia ovis have been shown to be highly effective when used as vaccines against cysticercosis in the intermediate hosts. This study investigated the presence of mRNA encoding the TSOL18 and TSOL16 antigens in different life-cycle stages of T. solium, and their related homologues in T. ovis. Reverse transcription-PCR and Southern blotting demonstrated that the antigens are stage-specifically expressed in the oncosphere. The apparent absence of expression of TSOL18 in the metacestode life-cycle stage suggests that the vaccine based on this antigen targets exclusively the early stages in the development of the parasite.
Gauci
Charles G
CG
University of Melbourne, Department of Veterinary Science, 250 Princes Highway, Werribee, Vic. 3030, Australia. charlesg@unimelb.edu.au
Verástegui
Manuela R
MR
Gilman
Robert H
RH
Lightowlers
Marshall W
MW
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D015139:Blotting, Southern; D003551:Cysticercosis / Q000517:prevention & control*; D015870:Gene Expression; D008018:Life Cycle Stages / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D013621:Taenia / Q000235:genetics / Q000276:immunology*; D041201:Taenia solium / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*
D000947:Antigens, Helminth; D012333:RNA, Messenger; C113407:TSOL-18 antigen; C512850:TSOL16 antigen, Taenia solium; D014614:Vaccines, Synthetic