Hormonal regulation and differential expression of neuropilin (NRP)-1 and NRP-2 genes in bovine granulosa cells.
Although much is known about the biology of vascular endothelial growth factor (VEGF) and its receptors, little is known about the role of the VEGF receptors neuropilin (NRP)-1 and NRP-2 in the process of bovine follicle development. The aim of the present study was to examine the hormonal regulation of NRP-1 and NRP-2 mRNAs by real-time PCR in follicles from the bovine ovary and in cultured granulosa cells. The NRP-1 gene was expressed in the granulosa and theca cells in the post-selection (POF) and pre-selection (PRF) follicles in the bovine ovary. In contrast, the NRP-2 gene was expressed only in the theca cells in the POF and the PRF. The level of NRP-1 mRNA was significantly increased by treatment of the cultured granulosa cells with 10 ng/ml estradiol (E2). In contrast, the addition of progesterone (P4) to the culture medium decreased the expression of the NRP-1 gene. The level of NRP-1 mRNA was increased by 10 ng/ml E2 with or without 1 ng/ml P4, but the level of NRP-1 mRNA was decreased if the P4 level was increased to 10 ng/ml, even when 1 ng/ml E2 was also added. Follicle-stimulating hormone did not stimulate the expression of the NRP-1 gene. These results are the first data showing that NRP-1, but not NRP-2, is expressed in the granulosa cells of bovine follicles and that NRP-1 gene expression is regulated by sex steroids. Our findings suggest the involvement of NRP-1 in follicle development in the cow.
Shimizu
Takashi
T
Graduate School of Animal and Food Hygiene and Department of Agriculture and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan. shimizut@obihiro.ac.jp
Jayawardana
Barana C
BC
Nishimoto
Hiromi
H
Kaneko
Etsushi
E
Tetsuka
Masafumi
M
Miyamoto
Akio
A
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005498:Follicular Phase; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D008183:Luteal Phase; D039942:Neuropilin-1 / Q000235:genetics*; D039943:Neuropilin-2 / Q000235:genetics*; D011374:Progesterone / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D039943:Neuropilin-2; D039942:Neuropilin-1; D011374:Progesterone; D004958:Estradiol