Conversion of Sphingobium chlorophenolicum ATCC 39723 to a hexachlorobenzene degrader by metabolic engineering.
The gene cassette (camA+ camB+ camC) encoding a cytochrome P-450cam variant was integrated into the nonessential gene pcpM of the pentachlorophenol degrader Sphingobium chlorophenolicum ATCC 39723 by homologous recombination. The recombinant strain could degrade hexachlorobenzene at a rate of 0.67 nmol.mg (dry weight)-1.h-1, and intermediate pentachlorophenol was also identified.
Yan
Da-Zhong
DZ
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
Liu
Hong
H
Zhou
Ning-Yi
NY
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D019475:Camphor 5-Monooxygenase / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D006581:Hexachlorobenzene / Q000378:metabolism*; D010416:Pentachlorophenol / Q000378:metabolism; D011995:Recombination, Genetic; D042301:Sphingomonadaceae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D006581:Hexachlorobenzene; D010416:Pentachlorophenol; D019475:Camphor 5-Monooxygenase