Up-regulation and increased phosphorylation of protein kinase C (PKC) delta, mu and theta in the degenerating rd1 mouse retina.
The rd1 mouse serves as a model for inherited photoreceptor degeneration: retinitis pigmentosa. Microarray techniques were employed to compare the transcriptomes of rd1 and congenic wild-type retinas at postnatal day 11, when degenerative processes have started but most photoreceptors are still present. Of the several genes that were differentially expressed, focus was put on those associated with the protein kinase C (PKC) signaling pathway, in particular PKCdelta, mu and theta. Microarray identified these as being up-regulated in the rd1 retina, which was confirmed by QRT-PCR. Western blotting and immunostaining, using antibodies against either total or phosphorylated variants of the PKC isoforms, revealed increased expression and phosphorylation of PKCdelta, mu and theta in the rd1 retina at the protein level as well. Our results suggest that these PKC isoforms are involved in rd1 degeneration.
Azadi
Seifollah
S
Department of Ophthalmology, Lund University, BMC-B13, SE-221 84 Lund, Sweden.
Paquet-Durand
Francois
F
Medstrand
Patrik
P
van Veen
Theo
T
Ekström
Per A R
PA
D000818:Animals; D019295:Computational Biology; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D051745:Protein Kinase C-delta / Q000235:genetics / Q000378:metabolism*; D000074121:Protein Kinase C-theta; D012160:Retina / Q000166:cytology / Q000473:pathology / Q000502:physiology; D012174:Retinitis Pigmentosa / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation
D007527:Isoenzymes; C497259:Prkcd protein, mouse; C089212:protein kinase D; C489593:Prkcq protein, mouse; D011493:Protein Kinase C; D051745:Protein Kinase C-delta; D000074121:Protein Kinase C-theta