Interleukin-1 alpha alters the expression of matrix metalloproteinases and collagen degradation by pulp fibroblasts.
Matrix metalloproteinases (MMPs) and the tissue inhibitors of MMPs (TIMPs) have been suggested to play a role in dental pulp destruction. This study examined the effects of interleukin (IL)-1 alpha on pulp fibroblasts. The ability of these cells to degrade collagen was determined with or without IL-1 alpha utilizing a cell-mediated collagen degradation assay. Reverse transcriptase-polymerase chain reaction was utilized to examine the mRNA expression of multiple MMPs and TIMPs with and without IL-1 alpha, while Western blot analyses and zymography were utilized to examine their protein expression. The collagen degradation mediated by these cells was stimulated by IL-1 alpha and inhibited by MMP inhibitors. IL-1 alpha increased the mRNA expression of MMP-1 and MMP-3, as well as induced MMP-7. Western blot analyses confirmed these results. IL-1 alpha increased the secreted protein level of TIMP-1, while only slightly affected the level of TIMP-2. These results suggest that IL-1 alpha can induce pulp destruction by differentially regulating MMPs and TIMPs.
Wisithphrom
Kessiri
K
Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, Indiana 46202, USA.
Murray
Peter E
PE
Windsor
L Jack
LJ
D000328:Adult; D015153:Blotting, Western; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003782:Dental Pulp / Q000166:cytology / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019714:Tissue Inhibitor of Metalloproteinases / Q000096:biosynthesis; D014746:Vimentin / Q000096:biosynthesis
D017077:Culture Media, Conditioned; D007375:Interleukin-1; D019714:Tissue Inhibitor of Metalloproteinases; D014746:Vimentin; D003094:Collagen; D020782:Matrix Metalloproteinases