title: Biodegradation of oxadiazon by a soil isolated Pseudomonas fluorescens strain CG5: Implementation in an herbicide removal reactor and modelling.
abstract: An oxadiazon-degrading bacterial, Pseudomonas strain CG5, was isolated from an agricultural contaminated soil. This strain CG5 was able to grow on 10mg of oxadiazon per l, yielding 5.18+/-0.2 mg of protein biomass mol(-1). GC-MS analyses of the metabolites from oxadiazon catabolism revealed its dehalogenation and degradation to form non-toxic end-products, cells were then immobilized by adsorption on a ceramic support to be used as biocatalysts in herbicide removal biofilm-reactor processes. Seventy-two per cent of the oxadiazon was removed, and the maximum specific substrate uptake rate was 10.63+/-0.5 microg h(-1) mg(-1) prot. A new mathematical model was developed to interpret and predict the behaviour of the bacteria and pollutants in a biofilm-reactor system, to consider biofilm structural and morphological properties.
authors.affiliation: Department Bioquímica y Biología Molecular IV, Universidad Complutense (UCM), Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Garbi
authors.forename: Luis
authors.initials: L
authors.lastname: Casasús
authors.forename: Roberto
authors.initials: R
authors.lastname: Martinez-Alvarez
authors.forename: Jose
authors.initials: J
authors.lastname: Ignacio Robla
authors.forename: Margarita
authors.initials: M
authors.lastname: Martín
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors; D006540:Herbicides / Q000378:metabolism*; D018613:Microscopy, Confocal; D008962:Models, Theoretical; D010069:Oxadiazoles / Q000378:metabolism*; D010865:Pilot Projects; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D006540:Herbicides; D010069:Oxadiazoles; D012989:Soil Pollutants; C012466:oxadiazon