Infectivity of porcine circovirus 1 and circovirus 2 in primary porcine hepatocyte and kidney cell cultures.
Infectivity of porcine circovirus (PCV) 1 and PCV2 was examined in primary porcine hepatocyte culture by comparing that of PCV in primary kidney cell culture. The virus titer of PCV2-infected hepatocyte cultures was higher than that of the PCV1-infected hepatocyte cultures and the PCV-infected kidney cell cultures. The number of virus-positive cells was most abundant in PCV2-infected hepatocyte cultures as determined by immunohistochemistry and/or in situ hybridization. The results of our data suggest that PCV2 preferably infects cultured hepatocytes as observed in the liver of pigs with postweaning multisystemic wasting syndrome.
Hirai
Takuya
T
Nippon Institute for Biological Science, 9-2221-1 Shinmachi, Ome, Tokyo 198-0024, Japan.
Nunoya
Tetsuo
T
Ihara
Takeshi
T
Saitoh
Toshiki
T
Shibuya
Kazumoto
K
Nakamura
Keigo
K
D000818:Animals; D000831:Animals, Newborn; D000956:Antigens, Viral / Q000302:isolation & purification; D002478:Cells, Cultured; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000276:immunology / Q000472:pathogenicity*; D022781:Hepatocytes / Q000821:virology*; D007150:Immunohistochemistry / Q000662:veterinary; D007668:Kidney / Q000166:cytology / Q000821:virology; D008107:Liver Diseases / Q000662:veterinary* / Q000821:virology; D008297:Male; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D013556:Swine, Miniature; D019282:Wasting Syndrome / Q000662:veterinary* / Q000821:virology
D000956:Antigens, Viral