IL-18 gene polymorphisms affect IL-18 production capability by monocytes.
We previously demonstrated a significant association between IL-18 gene polymorphism 105A/C and asthma. In this study, we investigated the relationship of IL-18 gene polymorphism to IL-18 production capability by monocytes. The frequency of gene polymorphisms including IL-18-105A/C and IL-18--137G/C was determined by PCR analyses. The IL-18 production by monocytes stimulated without or with LPS or A23187+PMA for 1day was measured by ELISA. The produced IL-18 spontaneously or in response to A23187+PMA by monocytes was significantly higher for volunteers with 105A/A genotype than with 105A/C genotype. Similarly, the production capability of IL-18 by monocytes from volunteers with -137G/G genotype was significantly higher than that with -137G/C genotype and significant linkage disequilibrium was observed between 105A/C and -137G/C polymorphism. Thus, the genetic capacity to produce more IL-18 in response to stimuli may affect the onset of asthma.
Arimitsu
Junsuke
J
Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Medical School, Osaka 565-0871, Japan.
Hirano
Toru
T
Higa
Shinji
S
Kawai
Mari
M
Naka
Tetsuji
T
Ogata
Atsushi
A
Shima
Yoshihito
Y
Fujimoto
Minoru
M
Yamadori
Tomoki
T
Hagiwara
Keisuke
K
Ohgawara
Tomoharu
T
Kuwabara
Yusuke
Y
Kawase
Ichiro
I
Tanaka
Toshio
T
D001249:Asthma / Q000235:genetics* / Q000276:immunology*; D005260:Female; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics* / Q000276:immunology*; D015810:Linkage Disequilibrium / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology*; D011110:Polymorphism, Genetic / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D020382:Interleukin-18