title: Quantification of Feline immunodeficiency virus (FIVpco) in peripheral blood mononuclear cells, lymph nodes and plasma of naturally infected cougars.
abstract: Infection of domestic cats with Feline immunodeficiency virus (FIV) results in a fatal immunodeficiency disease, similar to Human immunodeficiency virus 1 (HIV-1) in humans. Elevated plasma viral loads in domestic cats are correlated to decreased survival time and disease progression. However, FIV is also maintained as an apathogenic infection in other members of the family Felidae including cougars, Puma concolour (FIVpco). It is not known whether the lack of disease in cougars is a result of diminished virus replication. A real-time PCR assay was developed to quantify both FIVpco proviral and plasma viral loads in naturally infected cougars. Proviral loads quantified from peripheral blood mononuclear cells (PBMC) ranged from 2.90 x 10(1) to 6.72 x 10(4) copies per 10(6) cells. Plasma viral loads ranged from 2.30 x 10(3) to 2.81 x 10(6) RNA copies ml(-1). These data indicate that FIVpco viral loads are comparable to viral loads observed in endemic and epidemic lentivirus infections. Thus, the lack of disease in cougars is not due to low levels of virus replication. Moreover, significant differences observed among cougar PBMC proviral loads correlated to viral lineage and cougar age (P=0.014), which suggests that separate life strategies exist within FIVpco lineages. This is the first study to demonstrate that an interaction of lentivirus lineage and host age significantly effect proviral loads.
authors.affiliation: Division of Biological Sciences, University of Montana, HS104, Missoula, MT 59812, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Blake
authors.affiliation: Department of Mathematical Sciences, University of Montana, HS104, Missoula, MT 59812, USA.
authors.forename: Jon
authors.initials: J
authors.lastname: Graham
authors.affiliation: Division of Biological Sciences, University of Montana, HS104, Missoula, MT 59812, USA.
authors.forename: Mary
authors.initials: M
authors.lastname: Poss
mesh_terms: D000818:Animals; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016180:Lentivirus Infections / Q000662:veterinary* / Q000821:virology; D007963:Leukocytes, Mononuclear / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010949:Plasma / Q000821:virology; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D046011:Puma / Q000821:virology*; D017422:Sequence Analysis, DNA; D019562:Viral Load