title: Comparison of the molten globule states of thermophilic and mesophilic alpha-amylases.
abstract: In recent years great interest has been generated in the process of protein folding, and the formation of intermediates during the folding process has been proven with new experimental strategies. In the present work, we have examined the molten globule state of Bacillus licheniformis alpha-amylase (BLA) by intrinsic fluorescence and circular dichroism spectra, 1-anilino naphthalene-8-sulfonate (ANS) binding and proteolytic digestion by pepsin, for comparison to its mesophilic counterpart, Bacillus amyloliquefaciens alpha-amylase (BAA). At pH 4.0, both enzymes acquire partially folded state which show characteristics of molten globule state. They unfold in such a way that their hydrophobic surfaces are exposed to a greater extent compared to the native forms. Chemical denaturation studies by guanidine hydrochloride and proteolytic digestion with pepsin show that molten globule state of BLA is more stable than from BAA. Results from gel filtration indicate that BAA has the same compactness at pH 4.0 and 7.5. However, molten globule state of BLA is less compact than its native state. The effects of polyols such as trehalose, sorbitol and glycerol on refolding of enzymes from molten globule to native state were also studied. These polyols are effective on refolding of mesophilic alpha-amylase but only slightly effect on BLA refolding. In addition, the folding pathway and stability of intermediate state of the thermophilic and the mesophilic alpha-amylases are discussed.
authors.affiliation: Department of Biochemistry, Faculty of Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
authors.forename: Maryam Monsef
authors.initials: MM
authors.lastname: Shokri
authors.forename: Khosro
authors.initials: K
authors.lastname: Khajeh
authors.forename: Jahan
authors.initials: J
authors.lastname: Alikhajeh
authors.forename: Ahmad
authors.initials: A
authors.lastname: Asoodeh
authors.forename: Bijan
authors.initials: B
authors.lastname: Ranjbar
authors.forename: Saman
authors.initials: S
authors.lastname: Hosseinkhani
authors.forename: Mehdi
authors.initials: M
authors.lastname: Sadeghi
mesh_terms: D001407:Bacillus / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D017510:Protein Folding; D013997:Time Factors; D000516:alpha-Amylases / Q000737:chemistry*
chemical_list: D000516:alpha-Amylases