title: Proteomic analysis of mice hippocampus in simulated microgravity environment.
abstract: Space travel induces many deleterious effects on the flight crew due to the '0' g environment. The brain experiences a tremendous fluid shift, which is responsible for many of the detrimental changes in physical behavior seen in astronauts. It therefore indicates that the brain may undergo major changes in its protein levels in a '0' g environment to counteract the stress. Analysis of these global changes in proteins may explain to better understand the functioning of brain in a '0' g condition. Toward such an effort, we have screened proteins in the hippocampus of mice kept in simulated microgravity environment for 7 days and have observed a few changes in major proteins as compared to control mice. Essentially, the results show a major loss of proteins in the hippocampus of mice subjected to simulated microgravity. These changes occur in structural proteins such as tubulin, coupled with the loss of proteins involved in metabolism. This preliminary investigation leads to an understanding of the alteration of proteins in the hippocampus in response to the microgravity environment.
authors.affiliation: Molecular Neurotoxicology Laboratory and Proteomics Core, Department of Biology, Texas Southern University, Houston, Texas 77004, USA.
authors.forename: Poonam
authors.initials: P
authors.lastname: Sarkar
authors.forename: Shubhashish
authors.initials: S
authors.lastname: Sarkar
authors.forename: Vani
authors.initials: V
authors.lastname: Ramesh
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Hayes
authors.forename: Renard L
authors.initials: RL
authors.lastname: Thomas
authors.forename: Bobby L
authors.initials: BL
authors.lastname: Wilson
authors.forename: Helen
authors.initials: H
authors.lastname: Kim
authors.forename: Stephen
authors.initials: S
authors.lastname: Barnes
authors.forename: Anil
authors.initials: A
authors.lastname: Kulkarni
authors.forename: Neal
authors.initials: N
authors.lastname: Pellis
authors.forename: Govindarajan T
authors.initials: GT
authors.lastname: Ramesh
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000737:chemistry* / Q000378:metabolism*; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018474:Weightlessness Simulation; D051846:beta-Synuclein / Q000737:chemistry / Q000378:metabolism
chemical_list: D020543:Proteome; D011768:Pyruvate Dehydrogenase Complex; D051846:beta-Synuclein