The influence of adhesive and invasive properties of Staphylococcus aureus defective in fibronectin-binding proteins on secretion of interleukin-6 by human endothelial cells.
Fibronectin-binding proteins (FnBP) are surface adhesins of Staphylococcus aureus documented to be virulence attributes in, for example, endovascular infections. By using mutants of S. aureus defective in the FnBPA and B genes we have investigated whether these adhesins affect cytokine expression in human umbilical vein endothelial cells (HUVEC). S. aureus expressing FnBPA and B adhered to and were internalized into HUVEC to a greater extent compared to mutants defective in expression of FnBP. Production and release of IL-6 was higher from endothelial cells infected with the parent FnBP-expressing strain compared to the FnBP-defective mutants. These results indicate that adhesion to and invasion of S. aureus into endothelial cells are important regulators of cytokine expression.
Söderquist
Bo
B
Department of Infectious Diseases, University Hospital, Orebro, Sweden. bo.soderquist@orebroll.se
Alriksson
Ingegerd
I
Källman
Jan
J
Kihlström
Erik
E
D018829:Adhesins, Bacterial / Q000235:genetics* / Q000276:immunology; D001422:Bacterial Adhesion / Q000276:immunology / Q000502:physiology; D042783:Endothelial Cells / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism*; D009154:Mutation; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000254:growth & development / Q000276:immunology
D018829:Adhesins, Bacterial; D015850:Interleukin-6; C078635:fibronectin-binding proteins, bacterial