Mitochondrial DNA deletion in granulosa and cumulus oophorus cells.
The incidence of the 4,977-bp deletion in mitochondrial DNA (DeltamtDNA4977) in 73 pairs of granulosa and cumulus oophorus cells was studied with polymerase chain reaction (PCR), and was significantly higher in granulosa cells (GC) (67/73, 91%) than in cumulus oophorus cells (17/73, 23.3%), independent of the donors' age. In the cumulus oophorus cells, the oocyte morphology, the ooplasma diameter, and the proportion of oocytes fertilized normally were comparable between those with and without DeltamtDNA4977; whereas the oocyte diameter and the zona thickness were significantly higher in those with DeltamtDNA4977.
Chan
Carina C W
CC
Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Hong Kong. cwcchan@graduate.hku.hk
Liu
Vincent W S
VW
Lau
Estella Y L
EY
Yeung
William S B
WS
Ng
Ernest H Y
EH
Ho
Pak-Chung
PC
D000328:Adult; D000375:Aging / Q000502:physiology; D004272:DNA, Mitochondrial; D005260:Female; D017353:Gene Deletion; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D009865:Oocytes / Q000648:ultrastructure*; D006080:Ovarian Follicle / Q000166:cytology* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D015044:Zona Pellucida / Q000648:ultrastructure
D004272:DNA, Mitochondrial