Rapid PCR amplification of DNA utilizing Coriolis effects.
A novel polymerase chain reaction (PCR) method is presented that utilizes Coriolis and centrifugal effects, produced by rotation of the sample disc, in order to increase internal circulatory rates, and with them temperature homogenization and mixing speeds. A proof of concept has been presented by testing a rapid 45-cycle PCR DNA amplification protocol. During the repeated heating and cooling that constitutes a PCR process, the 100 microL samples were rotated at a speed equivalent to an effective acceleration of gravity of 7,000 g. A cycle time of 20.5 s gave a total process time of 15 min to complete the 45 cycles. A theoretical and numerical analysis of the resulting flow, which describes the increased mixing and temperature homogenization, is presented. The device gives excellent reaction speed efficiency, which is beneficial for rapid PCR.
Mårtensson
Gustaf
G
Department of Mechanics, Royal Institute of Technology (KTH), Stockholm, Sweden. gustaf@mech.kth.se.
Skote
Martin
M
Malmqvist
Mats
M
Falk
Mats
M
Asp
Allan
A
Svanvik
Nicke
N
Johansson
Arne
A
D003312:Coriolis Force; D004247:DNA / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012399:Rotation; D013696:Temperature
D004247:DNA