Identification of transcription complexes that contain the double bromodomain protein Brd2 and chromatin remodeling machines.
We use affinity purification of the double bromodomain protein Brd2 to isolate a multicomponent nuclear complex from cultured cells, and apply mass spectrometry/proteomics methods to identify the participants. We then confirm by immunoblot several transcription co-activators and co-repressors, proteins of the Swi/Snf chromatin remodeling complex, which regulate transcription control of cyclin A. This multiprotein complex is likely to contribute to cell cycle control and play a role in proliferation and cancer.
Denis
Gerald V
GV
Pharmacology and Medicine, Cancer Research Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA. gdenis@bu.edu
McComb
Mark E
ME
Faller
Douglas V
DV
Sinha
Anupama
A
Romesser
Paul B
PB
Costello
Catherine E
CE
D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D042002:Chromatin Assembly and Disassembly / Q000235:genetics; D019925:Cyclin A / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D017434:Protein Structure, Tertiary / Q000235:genetics; D014157:Transcription Factors; D014158:Transcription, Genetic; D000092468:Bromodomain Containing Proteins
D018797:Cell Cycle Proteins; D019925:Cyclin A; D046912:Multiprotein Complexes; D017346:Protein Serine-Threonine Kinases; D014157:Transcription Factors; C098415:BRD2 protein, human; D000092468:Bromodomain Containing Proteins