title: High resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell lines.
abstract: Tumours develop from clonally expanded population of cells harbouring aberrations of oncogenes and tumour suppressor genes. In this study, metaphase and array comparative genomic hybridization showed good correlation of aberration profiles in lung adenocarcinoma cell lines from patients with different tobacco exposure. Recurrent DNA gains were found at chromosomes 1, 7, 8, 17, 20, and deletions at 1, 3, 8, 9, 10, 12, 17, 18, 19. Candidate tumour loci and encompassed genes at 7p21 (AGR2), 8q21(TPD52), 20q13 (ZNF217, WFDC2, EEF1A2) and 10p15 (KLF6) were analyzed by dual colour FISH for genomic changes and quantitative PCR for expression changes. Results indicated that EEF1A2 and KLF6 were strong candidates of oncogene and tumour suppressor genes, respectively. This study illustrates, a practical strategy for identifying candidate cancer genes from microarray data.
authors.affiliation: Department of Pathology, Queen Mary Hospital, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.forename: David Chi Leung
authors.initials: DC
authors.lastname: Lam
authors.forename: Kam Chu
authors.initials: KC
authors.lastname: Han
authors.forename: Vicky Pui Chi
authors.initials: VP
authors.lastname: Tin
authors.forename: Wai Sing
authors.initials: WS
authors.lastname: Suen
authors.forename: Elaine
authors.initials: E
authors.lastname: Wang
authors.forename: Wah Kit
authors.initials: WK
authors.lastname: Lam
authors.forename: Wei Wen
authors.initials: WW
authors.lastname: Cai
authors.forename: Lap Ping
authors.initials: LP
authors.lastname: Chung
authors.forename: Maria Pik
authors.initials: MP
authors.lastname: Wong
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D002869:Chromosome Aberrations; D005260:Female; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008677:Metaphase / Q000235:genetics; D046228:Microarray Analysis / Q000379:methods; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured