[Differentiation of strains and isolates of chicken reovirus using PCR and heteroduplex mobility assay in amplified cDNA].
The method of chicken reovirus strain differentiation was worked out on the basis of RT-PCR and heteroduplex mobility assay (HMA). The S3 gene cDNA (633-896 b.p.) of some Russian and Italian chicken reovirus isolates was amplified by RT-PCR. The analysis of these cDNA samples was carried out by HMA. The relation between nucleotide differences and relative mobility of compared cDNA heteroduplex was reflected by the regression curve. The equation of linear regression was derived (y = 91.726-0.89x; where y is the level of nucleotide difference of compared cDNA (%), x is the relative mobility of compared cDNA heteroduplex (%)). This method made it possible to take correct results within 5-35% of nucleotide difference in heteroduplex sequences.
Andreĭchuk
D B
DB
Mudrak
D E
DE
Khitrova
L B
LB
Drygin
V V
VV
D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D002645:Chickens / Q000821:virology; D018076:DNA, Complementary / Q000032:analysis; D020180:Heteroduplex Analysis; D011201:Poultry Diseases / Q000821:virology*; D016601:RNA-Binding Proteins / Q000235:genetics; D012044:Regression Analysis; D012087:Reoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012088:Reoviridae Infections / Q000662:veterinary* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D036022:Capsid Proteins; D018076:DNA, Complementary; D016601:RNA-Binding Proteins; C053179:sigma protein 3, Reovirus