title: The effects of lateralized training on spontaneous forelimb preference, lesion deficits, and graft-mediated functional recovery after unilateral striatal lesions in rats.
abstract: The ability of striatal embryonic grafts to promote functional recovery on complex behavioral tasks depends on various factors, including the amount of striatal-like tissue within the grafts and the duration of post-graft training. However, how the innate paw bias of animals is affected by experience, or influences recovery following injury, is less known. Here, we have examined the effects of intrinsic side bias and lateralized limb use training on spontaneous forelimb preference and graft-mediated functional recovery in a skilled reaching task in a rodent model of Huntington's disease. Naïve rats were assessed on their baseline paw preferences when reaching between the bars of their cage to retrieve sugar pellets from a tray attached outside. Next, rats were lesioned unilaterally in the lateral dorsal striatum with quinolinic acid, and 7-10 days later, half of the animals were given suspension grafts prepared from E15 whole ganglionic eminence implanted into the lesioned striatum. The animals then received extensive unilateral training, either ipsi- or contralateral to the side of the lesion and graft in separate subgroups, on the 'staircase' task until asymptotic performance was obtained. As reported previously, the grafts alleviated lesion-induced deficits in retrieving pellets from the contralateral staircase. Spontaneous biases were then reassessed in the cage-reaching task. Irrespective of whether the animal received ipsilateral or contralateral staircase training, the unilateral lesions induced a significant shift in spontaneous bias towards the ipsilateral paw. Grafted animals showed a similar shift in bias if staircase training was given to the ipsilateral paw but showed no change in spontaneous bias (similar to controls) if they had received contralateral training during the post-transplantation period. The results suggest that striatal grafts can alleviate lesion-induced changes in their spontaneous side preferences, but only if they receive extensive training in the use of the contralateral limb, compatible with the notion that recovery is use-dependent.
authors.affiliation: Brain Repair Group, School of Biosciences, Cardiff University, Museum Avenue Box 911, Cardiff CF10 3US, UK. dobrossymd@cf.ac.uk
authors.forename: Máté D
authors.initials: MD
authors.lastname: Döbrössy
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Dunnett
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001930:Brain Injuries / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D016380:Brain Tissue Transplantation / Q000379:methods*; D003342:Corpus Striatum / Q000187:drug effects / Q000473:pathology / Q000637:transplantation*; D004195:Disease Models, Animal; D004622:Embryo, Mammalian; D016332:Fetal Tissue Transplantation / Q000379:methods; D007839:Functional Laterality / Q000502:physiology*; D006085:Graft Survival; D008297:Male; D010805:Physical Conditioning, Animal / Q000379:methods*; D011597:Psychomotor Performance / Q000502:physiology; D017378:Quinolinic Acid / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*; D013997:Time Factors; D034941:Upper Extremity / Q000503:physiopathology
chemical_list: D017378:Quinolinic Acid