[The effect of peroxynitrite on the affinity of hemoglobin for oxygen in vitro].
The aim of the study was to estimate the influence of peroxynitrite on the affinity of hemoglobin for oxygen in vitro. It was shown that the incubation of venous blood with peroxynitrite resulted in a rise of affinity of hemoglobin for oxygen. Presumably, this effect is realized through the generation of various forms of hemoglobin: heme-oxidized and modified at the amino acid residues of the protein. The dependence of the results of hemoglobin-peroxynitrite reaction on the carbon dioxide tension and the saturation of hemoglobin by oxygen is discussed.
D000818:Animals; D002021:Buffers; D004912:Erythrocytes / Q000187:drug effects; D006454:Hemoglobins / Q000737:chemistry* / Q000187:drug effects*; D006358:Hot Temperature; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D030421:Peroxynitrous Acid / Q000494:pharmacology*; D051381:Rats
D002021:Buffers; D006454:Hemoglobins; D030421:Peroxynitrous Acid; D010100:Oxygen