Effect of docosahexaenoic acid and alpha-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation.
The aim of the present experiment was to study the effect of fish oil and Vitamin E rich diets on semen production, sperm functions and composition in broiler breeders. The following parameters were measured: semen volume and concentration, sperm motility and viability, sperm susceptibility to induced peroxidation, sperm lipid and alpha-tocopherol contents. Dietary n-3 PUFA were successfully transferred into spermatozoan phospholipid by fish oil feeding according to the following main features: (a) the C22:6n-3 and C22:5n - 3 contents were increased, but C22:4n-6 remained the peculiar and major polyunsaturate; (b) the content and proportion of total PUFA did not change; (c) the proportional increase of n-3 PUFA was compensated by the decrease of n-6 PUFA, an increase in the proportion of n-9 fatty acids was also found. The sperm content of alpha-tocopherol was doubled increasing the dietary availability of the vitamin to 300 mg/kg of feed. The specific n-3 PUFA and Vitamin E enrichment of chicken sperm affected cell functions. Significant interactions between the two treatments were also found for some parameters. The best sperm quality condition in control sperm (rich mainly in n-6 PUFA) was found supplying 200mg Vitamin E/kg of feed to the male breeders, and in contrast in n-3 rich sperm supplying 300 mg Vitamin E/kg.
Cerolini
S
S
Department of Veterinary Science and Technology for Food Safety (VSA), University of Milan, via Trentacoste 2, 20134 Milan, Italy. silvia.cerolini@unimi.it
Zaniboni
L
L
Maldjian
A
A
Gliozzi
T
T
D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena / Q000187:drug effects; D000818:Animals; D002645:Chickens / Q000502:physiology*; D019587:Dietary Supplements; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000502:physiology*; D008055:Lipids / Q000032:analysis*; D008297:Male; D011786:Quality Control; D012661:Semen / Q000166:cytology*; D013094:Spermatozoa / Q000737:chemistry*; D024502:alpha-Tocopherol / Q000032:analysis / Q000494:pharmacology*
D008055:Lipids; D004281:Docosahexaenoic Acids; D024502:alpha-Tocopherol