High performance liquid chromatography-mass spectrometry for metabonomics: potential biomarkers for acute deterioration of liver function in chronic hepatitis B.
Metabonomics methods have been successfully applied to the drug discovery, toxicology, phytochemistry, and clinical fields. Here, we report a self-developed metabonomics platform which is based on high performance liquid chromatography-mass spectrometry (HPLC-MS) technique and applied to the investigation of acute deterioration of liver function in chronic hepatitis B to find the potential biomarkers. Sera from 50 healthy persons and 37 patients with acute deterioration of liver function in chronic hepatitis B were analyzed by HPLC-MS after removal of proteins. After de-noise, peak detection and peak alignment, the data of metabolites were fed to partial least squares discriminant analysis (PLS-DA) to find the potential biomarkers. According to the corresponding tandem mass results, several potential biomarkers were identified: Lysophosphatidyl Choline (LPC) C18:0, LPC C16:0, LPC C18:1, LPC C18:2, and glycochenodeoxycholic acid (GCDCA) (or its isomer glycodeoxycholic acid (GDCA)). On the basis of the relevant literature and pathway databases, the biological significance of the present study is discussed.
Yang
Jun
J
National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023 Dalian, China.
Zhao
Xinjie
X
Liu
Xiaoli
X
Wang
Chang
C
Gao
Peng
P
Wang
Jiangshan
J
Li
Lanjuan
L
Gu
Jianren
J
Yang
Shengli
S
Xu
Guowang
G
D000328:Adult; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005999:Glycochenodeoxycholic Acid; D019694:Hepatitis B, Chronic / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology*; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000503:physiopathology*; D008111:Liver Function Tests; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D053719:Tandem Mass Spectrometry
D015415:Biomarkers; D005999:Glycochenodeoxycholic Acid