[Determination of concentration and aggregate size in influenza virus preparations using the true UV-absorption spectra].
It is well-known that influenza virus (IV) preparations are characterized by very large contribution of light-scattering to their UV absorption spectra. With the help of so called extrapolation method we managed to measure true absorption spectra of IV preparations and to determine absorption coefficients (E0.1(1) (cm, 280)) for the intact IV virions and for IV subviral particles. These coefficients turned out to equal 1.26 +/- 0.17 and 0.96 +/- 0.11 for the virions and subviral particles respectively. The knowledge of exact IV concentration is necessary for quantitative physico-chemical studies of IV virions and their components. It is also shown that UV absorption spectra measurements allow to register IV virion aggregation. Aggregation properties of IV subviral particles were also studied.
Ksenofontov
A L
AL
Kozlovskiĭ
V S
VS
Kordiukova
L V
LV
Radiukhin
V A
VA
Timofeeva
A V
AV
Dobrov
E N
EN
D000818:Animals; D002642:Chick Embryo; D006863:Hydrogen-Ion Concentration; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays