Short communication: Inactivation of microbial contaminants in raw milk La Serena cheese by high-pressure treatments.
La Serena cheese, a Spanish variety made from Merino ewes' raw milk, has a high pH value, low salt content, and high moisture, conditions that are all favorable for growth and survival of contaminating microorganisms, including pathogens. To improve its microbiological quality and safety, high-pressure treatments at 300 or 400 MPa for 10 min at 10 degrees C were applied to 2 batches of La Serena cheese on d 2 or 50 of ripening. Cheese treated on d 2 at 300 MPa showed viable aerobic counts that were 0.99 log units lower than those for control cheese on d 3 and showed counts of enterococci, coagulase-positive staphylococci, gram-negative bacteria, and coliforms that were 2.05, 0.49, 3.14, and 4.13 log units lower, respectively, than control cheese. For cheese treated on d 2 at 400 MPa, the respective reductions in counts were 2.02, 2.68, 1.45, 3.96, and 5.50 log units. On d 60, viable aerobic counts in cheese treated on d 50 at 300 MPa were 0.50 log units lower than those in control cheese, and counts of enterococci, gram-negative bacteria, and coliforms were 1.37, 2.30, and 4.85 log units lower, respectively. For cheese treated on d 50 at 400 MPa, the respective reductions in counts were 1.29, 1.98, 4.47, and > 5 log units. High-pressure treatments at 300 or 400 MPa on d 2 or 50 reduced significantly the counts of undesirable microorganisms, improving the microbiological quality and safety of La Serena cheese immediately after treatment and at the end of the ripening period.
Arqués
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Departamento de Tecnología de Alimentos, INIA, Madrid, 28040, Spain.
Garde
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Gaya
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Medina
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Nuñez
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D000818:Animals; D002611:Cheese / Q000382:microbiology*; D015169:Colony Count, Microbial; D004755:Enterobacteriaceae / Q000254:growth & development; D016983:Enterococcus / Q000254:growth & development; D005511:Food Handling / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000737:chemistry / Q000382:microbiology; D011312:Pressure; D012756:Sheep; D013211:Staphylococcus aureus / Q000254:growth & development; D013997:Time Factors