Protective effects of IL4-589T and RANTES-28G on HIV-1 disease progression in infected Thai females.
OBJECTIVE
To evaluate the effect of polymorphisms in interleukin-4 (IL4) and RANTES promoters on disease progression in HIV-1 infected Thais.


METHODS
Antiretroviral (ARV) drug-free HIV-1 infected females from the prospective cohort.


METHODS
A total of 246 DNA samples were genotyped for IL4 and RANTES promoter polymorphisms by PCR-RFLP. Associations of genotype with HIV-1 disease progression were assessed with respect to baseline clinical data including plasma HIV-1 load, CD4 cell counts, and proportion of symptomatic/AIDS, and survival status during 3 years of follow-up.


RESULTS
Patients with homozygous IL4-589T allele showed a significantly lower HIV-1 viral load (P = 0.005) and a higher CD4 cell count (P = 0.003) than the other patients with heterozygous IL4-589C/T or homozygous IL4-589C allele. Kaplan-Meier analysis demonstrated an apparent but insignificant trend towards better survival in homozygous IL4-589T patients. On the other hand, patients with RANTES-28G allele showed a significantly better survival while those with RANTES In1.1C allele without RANTES-28G showed a significantly poorer survival compared with those who did not possess either RANTES In1.1C or RANTES-28G (P = 0.02), although those polymorphisms only weakly associated with baseline viral load and CD4 cell counts.


CONCLUSIONS
Our results implicate the significant protective effect of IL4-589T and RANTES-28G on HIV disease progression in Thais. In contrast, RANTES In1.1C without RANTES-28G had an accelerating effect on HIV disease progression.
Wichukchinda
Nuanjun
N
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
Nakayama
Emi E
EE
Rojanawiwat
Archawin
A
Pathipvanich
Panita
P
Auwanit
Wattana
W
Vongsheree
Suthon
S
Ariyoshi
Koya
K
Sawanpanyalert
Pathom
P
Shioda
Tatsuo
T
D018791:CD4 Lymphocyte Count; D018946:Chemokine CCL5 / Q000235:genetics*; D018450:Disease Progression; D004812:Epidemiologic Methods; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology / Q000821:virology; D020276:HIV Long-Term Survivors; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D011379:Prognosis; D019562:Viral Load
D018946:Chemokine CCL5; D015847:Interleukin-4