title: Induction of zinc transporters by forskolin in human trophoblast BeWo cells.
abstract: During pregnancy, the zinc level in fetal serum is up to two-fold higher than that in the maternal serum at the end of pregnancy, but the mechanism of zinc release from the placenta into fetal circulation is not well understood. In this study, we determined the expression profiles of zinc transporters in human trophoblast BeWo cells, a representative human trophoblast cell line. Zn transporter 1-8 (ZnT1-8), Zrt/IRT-like protein 1 and Zn transporter-like transporter 1 were detected in BeWo cells by reverse transcription-polymerase chain reaction. Forskolin (FK) is a representative inducer of differentiation of BeWo cells cytotrophoblast into syncytiotrophoblast. Treatment of BeWo cells with FK resulted in morphological changes of BeWo cells into syncytiotrophoblast cells and secretion of human chorionic gonadotropin, which is a characteristic of syncytiotrophoblast cells. Treatment of BeWo cells with FK elevated ZnT1, 2 and 4 mRNA levels. These data about expression profiles of ZnTs may be useful for further investigation of placental biology.
authors.affiliation: Department of Biopharmaceutics and Pharmaceutics, Showa Pharmaceutical University, Machida-shi, Tokyo 194-8543, Japan.
authors.forename: Nagayoshi
authors.initials: N
authors.lastname: Asano
authors.forename: Masuo
authors.initials: M
authors.lastname: Kondoh
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ebihara
authors.forename: Makiko
authors.initials: M
authors.lastname: Fujii
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Naoki
authors.initials: N
authors.lastname: Utoguchi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Enomoto
authors.forename: Keiichi
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D027682:Cation Transport Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015854:Up-Regulation; D015032:Zinc
chemical_list: D027682:Cation Transport Proteins; D006063:Chorionic Gonadotropin; D012333:RNA, Messenger; C498299:SLC30A1 protein, human; C491597:SLC30A4 protein, human; D005576:Colforsin; D015032:Zinc