title: Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I.
abstract: Crystals of site-specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 A resolution at 100 K. The crystal belongs to space group P2(1), with unit-cell parameters a = 57.76, b = 90.67, c = 71.71, beta = 110.1 degrees. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume.
authors.affiliation: Max-Planck Research Unit for Structural Biology, Protein Dynamics Group, Hamburg 22607, Germany. galina@mpghdb.desy.de
authors.forename: Galina S
authors.initials: GS
authors.lastname: Kachalova
authors.forename: Eugeny A
authors.initials: EA
authors.lastname: Rogulin
authors.forename: Rimma I
authors.initials: RI
authors.lastname: Artyukh
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Perevyazova
authors.forename: Ludmila A
authors.initials: LA
authors.lastname: Zheleznaya
authors.forename: Nickolay I
authors.initials: NI
authors.lastname: Matvienko
authors.forename: Hans D
authors.initials: HD
authors.lastname: Bartunik
mesh_terms: D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D013379:Substrate Specificity; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D003850:Deoxyribonuclease I