PIP aquaporin gene expression in arbuscular mycorrhizal Glycine max and Lactuca sativa plants in relation to drought stress tolerance.
Although the discovery of aquaporins in plants has resulted in a paradigm shift in the understanding of plant water relations, the relationship between aquaporins and plant responses to drought still remains elusive. Moreover, the contribution of aquaporin genes to the enhanced tolerance to drought in arbuscular mycorrhisal (AM) plants has never been investigated. Therefore, we studied, at a molecular level, whether the expression of aquaporin-encoding genes in roots is altered by the AM symbiosis as a mechanism to enhance host plant tolerance to water deficit. In this study, genes encoding plasma membrane aquaporins (PIPs) from soybean and lettuce were cloned and their expression pattern studied in AM and nonAM plants cultivated under well-watered or drought stressed conditions. Results showed that AM plants responded to drought stress by down-regulating the expression of the PIP genes studied and anticipating its down-regulation as compared to nonAM plants. The possible physiological implications of this down-regulation of PIP genes as a mechanism to decrease membrane water permeability and to allow cellular water conservation is further discussed.
Porcel
Rosa
R
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Granada, 18008, Spain.
Aroca
Ricardo
R
Azcón
Rosario
R
Ruiz-Lozano
Juan Manuel
JM
D020346:Aquaporins / Q000737:chemistry* / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D004190:Disasters; D015536:Down-Regulation; D015966:Gene Expression Regulation, Fungal; D018506:Gene Expression Regulation, Plant; D005800:Genes, Fungal; D017343:Genes, Plant; D018545:Lactuca / Q000235:genetics* / Q000378:metabolism; D050356:Lipid Metabolism; D018515:Plant Leaves; D018517:Plant Roots / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012987:Soil; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism; D013559:Symbiosis; D013997:Time Factors; D014867:Water / Q000737:chemistry
D020346:Aquaporins; D017931:DNA Primers; D018076:DNA, Complementary; D012987:Soil; D014867:Water; D012313:RNA