title: Sophorolipid produced from the new yeast strain Wickerhamiella domercqiae induces apoptosis in H7402 human liver cancer cells.
abstract: The effects of sophorolipid on the growth and apoptosis of H7402 human liver cancer cells were investigated. By treatment with sophorolipid, a dose- and time-dependent inhibition of cell proliferation was observed. The cells developed many features of apoptosis, including condensation of chromatin, nuclear fragmentation, and appearance of apoptotic bodies, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling positive cells were stained dark brown. Sophorolipid treatment induced apoptosis in H7402 cells by blocking cell cycle at G1 phase and partly at S phase, activating caspase-3, and increasing Ca2+ concentration in cytoplasm. These findings may suggest a potential use of sophorolipid for liver cancer treatment.
authors.affiliation: State Key Lab of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Lab of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China. songx@sdu.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Song
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan, 250100, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Lab of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Yin-Bo
authors.initials: YB
authors.lastname: Qu
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan, 250100, China.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism* / Q000494:pharmacology*; D017209:Apoptosis; D001203:Ascomycota / Q000378:metabolism; D002118:Calcium / Q000032:analysis; D053148:Caspase 3 / Q000032:analysis; D045744:Cell Line, Tumor; D002470:Cell Survival; D002843:Chromatin / Q000378:metabolism; D003124:Colorimetry; D003593:Cytoplasm / Q000737:chemistry; D053938:DNA Fragmentation; D005470:Fluorometry; D016193:G1 Phase / Q000187:drug effects; D006017:Glycolipids / Q000096:biosynthesis* / Q000494:pharmacology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology*; D046529:Microscopy, Electron, Transmission; D008858:Microscopy, Phase-Contrast; D016196:S Phase / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D002843:Chromatin; D006017:Glycolipids; C067958:16-(2'-O-glucopyranosylglucopyranosyloxy)hexadecanoic acid 1',4''-lactone 6',6''-diacetate; D053148:Caspase 3; D002118:Calcium