title: Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans.
abstract: CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A, and diffract to 2.2 A resolution using synchrotron radiation.
authors.affiliation: Institut de Recherches Microbiologiques JM Wiame, Belgium.
authors.forename: Catherine
authors.initials: C
authors.lastname: Tricot
authors.forename: Sebastien
authors.initials: S
authors.lastname: van Aelst
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
authors.forename: Max
authors.initials: M
authors.lastname: Mergeay
authors.forename: Victor
authors.initials: V
authors.lastname: Stalon
authors.forename: Johan
authors.initials: J
authors.lastname: Wouters
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D042521:Burkholderiaceae / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D015394:Molecular Structure
chemical_list: D001426:Bacterial Proteins