Kinetic oxygen measurements by CVC96 in L-929 cell cultures.
Generally animal and human cells use oxygen during their whole life. Consequently the oxygen use is a simple indicator to test the vitality of cells. When the vitality decreases by the delivery of toxic substances the decrease can be observed directly by the oxygen-use of the cells. To get fast information of the vitality of cells we have measured the O2-tension by testing a new model of a bioreactor, the Cell Vitality Checker 96 (CVC96), in practical application. With this CVC96, soon a simple test will exist for the measurement of the oxygen use. In this respect the question had to be answered whether the use in the laboratory is easy and whether oxygen as a parameter in the vitality test can also be applied in future for problems in the field of material testing.
Plate
Ulrich
U
Department of Cranio-Maxillofacial Surgery, University of Münster, Waldeyerstrasse, Münster, Germany. plateu@uni-muenster.de
Polifke
Tobias
T
Sommer
Dieter
D
Wünnenberg
Jörg
J
Wiesmann
Hans-Peter
HP
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D007739:L Cells; D008163:Luminescent Measurements / Q000379:methods; D051379:Mice; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*
D003470:Culture Media; D010100:Oxygen