Phytosterol-mediated inhibition of intestinal cholesterol absorption is independent of ATP-binding cassette transporter A1.
An increased activity of ATP-binding cassette transporter (ABC) A1 has been proposed as a mechanism underlying the hypocholesterolaemic effect of phytosterols. In the present study, ABCA1-deficient mice (ABCA1-/- mice) were used to examine the involvement of the ABCA1 in the reduction of intestinal cholesterol absorption in response to a phytosterol-enriched diet. A decrease in intestinal cholesterol absorption of 39 and 35 % was observed after phytosterol treatment in ABCA1+/+ mice and in ABCA1-/- mice, respectively. No statistically significant changes in plasma lipoprotein profile or in intestinal ABCG5, ABCG8 and Niemann-Pick C1-Like 1 gene expression levels were found when phytosterol-treated ABCA1-/- mice and untreated ABCA1-/- mice were compared. We conclude that phytosterol inhibition of cholesterol absorption in mice is independent of ABCA1.
Calpe-Berdiel
Laura
L
Institut de Recerca, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
Escolà-Gil
Joan Carles
JC
Blanco-Vaca
Francisco
F
D064286:ATP Binding Cassette Transporter 1; D000071018:ATP Binding Cassette Transporter, Subfamily G, Member 5; D000071019:ATP Binding Cassette Transporter, Subfamily G, Member 8; D018528:ATP-Binding Cassette Transporters / Q000032:analysis / Q000378:metabolism*; D000042:Absorption; D000818:Animals; D002791:Cholesterol, Dietary / Q000493:pharmacokinetics*; D019587:Dietary Supplements; D015870:Gene Expression; D007413:Intestinal Mucosa / Q000378:metabolism*; D008074:Lipoproteins / Q000032:analysis; D026901:Membrane Transport Proteins / Q000032:analysis; D051379:Mice; D008811:Mice, Inbred DBA; D010840:Phytosterols / Q000008:administration & dosage*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
C462600:ABCG5 protein, mouse; C462601:ABCG8 protein, mouse; D064286:ATP Binding Cassette Transporter 1; D000071018:ATP Binding Cassette Transporter, Subfamily G, Member 5; D000071019:ATP Binding Cassette Transporter, Subfamily G, Member 8; D018528:ATP-Binding Cassette Transporters; D002791:Cholesterol, Dietary; D008074:Lipoproteins; D026901:Membrane Transport Proteins; C482445:Npc1l1 protein, mouse; D010840:Phytosterols