Spying the neutralizing epitopes on E2 N-terminal by candidate epitope-vaccines against classical swine fever virus.
Our previous study proved that the N-terminal (aa693-711) of glycoprotein E2 contained sequential neutralizing epitopes. In this study, four candidate epitope-vaccines (EVs) were separately prepared and evaluated. Among them, epitope-vaccine EV-BC1a (BC1a: aa693-699) induced high level of epitope-specific neutralizing antibodies and exhibited similar protective capability with that induced by Chinese vaccine strain (C-strain). These results confirmed CKEDYRY (aa693-699) as a principal sequential neutralizing epitope on E2 N-terminal. Moreover, these findings also indicate that epitope-vaccine is a potent candidate strategy for marker vaccine against classical swine fever virus (CSFV).
Dong
Xiao-Nan
XN
Laboratory of Immunology, Department of Biology, Tsinghua University, Protein Science Laboratory of the Ministry of Education, Beijing 100084, PR China. xndong00@mails.tsinghua.edu.cn
Chen
Ying-Hua
YH
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D006691:Classical Swine Fever / Q000276:immunology / Q000517:prevention & control; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000235:genetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D034421:Sus scrofa; D022581:Vaccines, Marker / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes; D022581:Vaccines, Marker; D022223:Vaccines, Subunit; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C087086:glycoprotein E2, classical swine fever virus