title: Proteome of the bacterium Mycoplasma penetrans.
abstract: A proteome map of Mycoplasma penetrans has been constructed using two-dimensional gel electrophoresis in combination with mass spectrometry (MS). Mycoplasma penetrans infects the urogenital and respiratory tracts of humans. A total of 207 spots were characterized with MS and, in comparing the experimental data with the DNA sequence-derived predictions, it was possible to assign these 207 spots to 153 genes. The Pro-Q Diamond phosphoprotein dye technology was used for the fluorescent detection of 26 phosphoproteins in the 4-7 pH range.
authors.affiliation: Institut de Biotecnología i de Biomedicina and Departament de Bioquímica i Biología Molecular, Universitat Autonoma de Barcelona. 08193, Bellaterra, Barcelona, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: Ferrer-Navarro
authors.forename: Antonio
authors.initials: A
authors.lastname: Gómez
authors.forename: Oscar
authors.initials: O
authors.lastname: Yanes
authors.forename: Raquel
authors.initials: R
authors.lastname: Planell
authors.forename: Francesc Xavier
authors.initials: FX
authors.lastname: Avilés
authors.forename: Jaume
authors.initials: J
authors.lastname: Piñol
authors.forename: Josep Antoni
authors.initials: JA
authors.lastname: Pérez Pons
authors.forename: Enrique
authors.initials: E
authors.lastname: Querol
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D018661:Mycoplasma penetrans / Q000737:chemistry* / Q000235:genetics; D010449:Peptide Mapping; D020543:Proteome / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D020543:Proteome