Use of enzyme-linked immunospot assay with Mycobacterium tuberculosis-specific peptides for diagnosis of recent infection with M. tuberculosis after accidental laboratory exposure.
This report of an accidental exposure to Mycobacterium tuberculosis in a microbiological laboratory illustrates the value of gamma interferon enzyme-linked immunospot assay using peptides of ESAT-6, CFP-10, TB37.6, and TB7.7 for the diagnosis of latent infection. In particular, positive responses to peptides 2 to 6 of TB37.6 were observed exclusively in recently infected persons.
Leyten
Eliane M S
EM
Department of Infectious Diseases, C5P, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
Mulder
Bert
B
Prins
Corine
C
Weldingh
Karin
K
Andersen
Peter
P
Ottenhoff
Tom H M
TH
van Dissel
Jaap T
JT
Arend
Sandra M
SM
D000062:Accidents, Occupational; D001426:Bacterial Proteins / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007753:Laboratories; D008297:Male; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D009426:Netherlands; D009784:Occupational Diseases / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology; D016273:Occupational Exposure; D010455:Peptides / Q000302:isolation & purification; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000209:etiology* / Q000382:microbiology
D001426:Bacterial Proteins; D010455:Peptides