Micellar gradients in size-exclusion simulated moving bed chromatography.
The selectivity of size-exclusion chromatography (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatography (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatography with the non-ionic surfactant C12E23 as gradient forming solute, and demonstrated by applying it to a relevant chromatographic protein separation problem.
Horneman
D A
DA
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Ottens
M
M
Keurentjes
J T F
JT
van der Wielen
L A M
LA
D002850:Chromatography, Gel / Q000379:methods*; D019281:Dimerization; D007074:Immunoglobulin G / Q000737:chemistry / Q000302:isolation & purification; D008823:Micelles
D007074:Immunoglobulin G; D008823:Micelles