title: A new taxoid from a callus culture of Taxus cuspidata as an MDR reversal agent.
abstract: A new taxoid, 5alpha,13alpha-diacetoxy-10beta-cinnamoyloxy-4(20),11-taxadien-9alpha-ol (1) along with its 9,10-isomer, taxinine NN-11 (2) were isolated from the callus cultures of Taxus cuspidata. The structures were identified by the analyses of the spectral data and chemical method. Their in vitro cytotoxicity against 3 cell lines (HepG2, WI-38 and VA-13) and multidrug resistance (MDR) reversal activity toward 2780AD tumor cells were preliminarily evaluated, the low cytotoxicities and potent MDR reversal activities suggested that they might be good lead compounds of tumor MDR reversal agent.
authors.affiliation: Department of Chemistry and Chemical Engineering, Niigata University, Ikarashi, Japan. jgdai@imm.ac.cn
authors.forename: Jungui
authors.initials: J
authors.lastname: Dai
authors.forename: Jiao
authors.initials: J
authors.lastname: Bai
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Shigenori
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Junichi
authors.initials: J
authors.lastname: Sakai
authors.forename: Seiko
authors.initials: S
authors.lastname: Oka
authors.forename: Miwa
authors.initials: M
authors.lastname: Kiuchi
authors.forename: Katutoshi
authors.initials: K
authors.lastname: Hirose
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tomida
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuruo
authors.forename: Minjie
authors.initials: M
authors.lastname: Li
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ando
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D043823:Taxoids / Q000737:chemistry* / Q000494:pharmacology*; D020946:Taxus / Q000737:chemistry*; D014700:Verapamil / Q000494:pharmacology
chemical_list: C510517:5alpha,13alpha-diacetoxy-10beta-cinnamoyloxy-4(20),11-taxadien-9alpha-ol; D000972:Antineoplastic Agents, Phytogenic; D002121:Calcium Channel Blockers; D005452:Fluoresceins; D043823:Taxoids; D014700:Verapamil; C007740:fluorexon