title: Isolation of the genome sequence strain Mycobacterium avium 104 from multiple patients over a 17-year period.
abstract: The genome sequence strain 104 of the opportunistic pathogen Mycobacterium avium was isolated from an adult AIDS patient in Southern California in 1983. Isolates of non-paratuberculosis M. avium from 207 other patients in Southern California and elsewhere were examined for genotypic identity to strain 104. This process was facilitated by the use of a novel two-step approach. In the first step, all 208 strains in the sample were subjected to a high-throughput, large sequence polymorphism (LSP)-based genotyping test, in which DNA from each strain was tested by PCR for the presence or absence of 4 hypervariable genomic regions. Nineteen isolates exhibited an LSP type that resembled that of strain 104. This subset of 19 isolates was then subjected to high-resolution repetitive sequence-based PCR typing, which identified 10 isolates within the subset that were genotypically identical to strain 104. These isolates came from 10 different patients at 5 clinical sites in the western United States, and they were isolated over a 17-year time span. Therefore, the sequenced genome of M. avium strain 104 has been associated with disease in multiple patients in the western United States. Although M. avium is known for its genetic plasticity, these observations also show that strains of the pathogen can be genotypically stable over extended time periods.
authors.affiliation: Seattle Biomedical Research Institute, 307 Westlake Avenue N, Suite 500, Seattle, Washington 98109, USA. kthoran@u.washington.edu
authors.forename: Kathleen L
authors.initials: KL
authors.lastname: Horan
authors.forename: Robert
authors.initials: R
authors.lastname: Freeman
authors.forename: Kris
authors.initials: K
authors.lastname: Weigel
authors.forename: Makeda
authors.initials: M
authors.lastname: Semret
authors.forename: Stacy
authors.initials: S
authors.lastname: Pfaller
authors.forename: Terry C
authors.initials: TC
authors.lastname: Covert
authors.forename: Dick
authors.initials: D
authors.lastname: van Soolingen
authors.forename: Sylvia C
authors.initials: SC
authors.lastname: Leão
authors.forename: Marcel A
authors.initials: MA
authors.lastname: Behr
authors.forename: Gerard A
authors.initials: GA
authors.lastname: Cangelosi
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D008040:Genetic Linkage; D016680:Genome, Bacterial; D006801:Humans; D017720:Molecular Epidemiology; D015269:Mycobacterium avium Complex / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology; D009894:Opportunistic Infections / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial