title: The pressure effect on the structure and functions of protein disulfide isomerase.
abstract: Studying on the pressure effects of the structure and functions of the multidomain protein, protein disulfide isomerase (PDI), the intrinsic Trp fluorescence spectra of PDI were measured under high pressure. PDI has 5 Trp residues and the two of all Trp residues are located at the neighborhood of the active site (WCGHC) for isomerase activity. On the basis of the red shift of center of spectral mass (CSM) of the intrinsic Trp fluorescence and the decrease in its fluorescence intensity, the changes in tertiary structure of PDI were observed above 100 MPa. These structural changes were completed at 400 MPa. The CSM of 400 MPa denatured PDI was comparable to that of 6.0 M GdnHCl denatured one. All of the Trp residues included in PDI are completely exposed to aqueous medium at 400 MPa. However, there is the significant difference between the pressure and GdnHCl-denatured PDI. The Trp fluorescence intensity was decreased with increasing pressure, but increased with the increase of the GdnHCl concentration. It is implied that the pressure-denatured state of PDI might remain compact not to be extensively unfolded. In the point of view about the reversibility of pressure-treated PDI, the tertiary structure was completely recovered after released to ambient pressure. The disulfide reduction and chaperone activity of 400 MPa-treated PDI were also recovered to be comparable to those of native one. Despite of a multidomain protein, the excellence in both structural and functional recovery of pressure-denatured PDI is quite remarkable. These unique properties of PDI against high pressure provide the insights into understanding the pressure-induced denaturation of PDI.
authors.affiliation: Department of Applied Chemistry, College of Science and Engineering, Ritsumeikan University, 1-1-1, Noji-higashi, Kusatsu, Shiga 525-8577, Japan. taniguti@se.ritsumei.ac.jp
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ado
authors.forename: Naohiro
authors.initials: N
authors.lastname: Takeda
authors.forename: Masakazu
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D005453:Fluorescence; D019803:Glutathione Disulfide / Q000737:chemistry; D019791:Guanidine / Q000737:chemistry; D018832:Molecular Chaperones / Q000737:chemistry*; D011312:Pressure; D011487:Protein Conformation; D011489:Protein Denaturation; D019704:Protein Disulfide-Isomerases / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry
chemical_list: D018832:Molecular Chaperones; D014364:Tryptophan; D019704:Protein Disulfide-Isomerases; D019791:Guanidine; D019803:Glutathione Disulfide