Presence of mitomycin-C in the anterior chamber after photorefractive keratectomy.
OBJECTIVE
To assess the presence of mitomycin-C (MMC) in hen aqueous humor after photorefractive keratectomy (PRK).


METHODS
Instituto Universitario de Oftalmobiología Aplicada, Faculty of Medicine, University of Valladolid, and Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Valladolid, Spain.


METHODS
Mitomycin-C 0.02% was applied topically for 2 minutes to a right hen's eye after PRK (Group A) and to the left eye with intact epithelium (Group B). At different time points (10, 30, 60, 360, and 720 minutes), aqueous humor was extracted and high-performance liquid chromatography was performed to detect and quantify MMC levels.


RESULTS
The mean maximum drug concentration of MMC measured in the aqueous humor was 187.250 microg/L +/- 4.349 (SD) in Group A and 93.000 +/- 4.899 microg/L in Group B, both detected 10 minutes after topical application. Statistically significant differences were found between Groups A and B at 10, 30, and 60 minutes, with decreasing MMC levels in both groups but a higher concentration in Group A. After 360 minutes, MMC levels were undetectable in Group B and after 720 minutes in Group A.


CONCLUSIONS
Mitomycin-C was detectable in the aqueous humor of the hen eye after topical application in PRK-treated eyes and in eyes with intact epithelium. The presence of MMC is of concern as it may lead to ocular toxicity in the long term.
Torres
Rodrigo M
RM
Instituto Universitario de Oftalmobiología Aplicada, Universidad de Valladolid, Spain.
Merayo-Lloves
Jesús
J
Daya
Sheraz M
SM
Blanco-Mezquita
José T
JT
Espinosa
Marcela
M
Nozal
Maria J
MJ
Bernal
José L
JL
Bernal
José
J
D000287:Administration, Topical; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics*; D001082:Aqueous Humor / Q000378:metabolism*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003315:Cornea / Q000378:metabolism* / Q000601:surgery; D054018:Lasers, Excimer; D016685:Mitomycin / Q000493:pharmacokinetics*; D018903:Photorefractive Keratectomy
D000903:Antibiotics, Antineoplastic; D016685:Mitomycin