title: Expression profiles of elastase1 (NvElastaseI) and secretory leukocyte protease inhibitor (NvSLPI) during forelimb regeneration in adult Notophthalmus viridescens suggest a role in epithelial remodeling and delamination.
abstract: Extracellular proteases and their inhibitors may regulate a number of important processes involved in forelimb regeneration in the adult newt, including epithelial remodeling, breakdown of extracellular matrix, and dedifferentiation. We have identified a newt homologue of human ElastaseI (NvElastaseI) and its potential inhibitor, SLPI (NvSLPI), and evaluated their spatial and temporal expression during limb regeneration. NvElastaseI is upregulated early in regeneration and is associated with subdermal and wound epithelial cells, suggesting an involvement in wound healing and the generation of the wound epithelium. Up until 15 days post-amputation, NvElastaseI is also scattered throughout the developing blastema and may have a role in the dedifferentiation of stump tissues. NvSLPI is found at the interface between the intact skin and the wound epithelium, and may limit NvElastaseI activity. NvSLPI is also expressed in dermal glands, and is likely involved in anti-microbial activity or function. Quite apart from regeneration, complementary patterns of expression of NvElastaseI and NvSLPI are associated with newt epithelial sloughing.
authors.affiliation: University of Ottawa Eye Institute,Ottawa Hospital, General Division, Ottawa, ON, Canada.
authors.forename: Sandy Gian
authors.initials: SG
authors.lastname: Vascotto
authors.forename: Shawn
authors.initials: S
authors.lastname: Beug
authors.forename: Richard A
authors.initials: RA
authors.lastname: Liversage
authors.forename: Catherine
authors.initials: C
authors.lastname: Tsilfidis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004848:Epithelium / Q000502:physiology; D005552:Forelimb / Q000502:physiology*; D015870:Gene Expression; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009673:Notophthalmus viridescens / Q000235:genetics / Q000502:physiology*; D010196:Pancreatic Elastase / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012038:Regeneration / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D053481:Secretory Leukocyte Peptidase Inhibitor; D010196:Pancreatic Elastase