Silencing of filamin A gene expression inhibits Ca2+ -sensing receptor signaling.
Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca2+ -sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function.
Huang
Chunfa
C
Division of Nephrology, Department of Medicine, Case Western Reserve University, Louis Stokes Veteran Affairs Medical Center, 10701 East Blvd., 151W, Cleveland, OH 44106, USA. chunfa.huang@case.edu
Wu
Zhenzhen
Z
Hujer
Kristine M
KM
Miller
R Tyler
RT
D002460:Cell Line; D003285:Contractile Proteins / Q000235:genetics* / Q000502:physiology; D064448:Filamins; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006801:Humans; D020935:MAP Kinase Signaling System; D008840:Microfilament Proteins / Q000235:genetics* / Q000502:physiology; D034741:RNA, Small Interfering / Q000494:pharmacology; D044169:Receptors, Calcium-Sensing / Q000037:antagonists & inhibitors*; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors; D015398:Signal Transduction / Q000235:genetics*
D003285:Contractile Proteins; D064448:Filamins; D008840:Microfilament Proteins; D034741:RNA, Small Interfering; D044169:Receptors, Calcium-Sensing; D043562:Receptors, G-Protein-Coupled