title: Compact soft x-ray transmission microscopy with sub-50 nm spatial resolution.
abstract: In this paper, the development of compact transmission soft x-ray microscopy (XM) with sub-50 nm spatial resolution for biomedical applications is described. The compact transmission soft x-ray microscope operates at lambda = 2.88 nm (430 eV) and is based on a tabletop regenerative x-ray source in combination with a tandem ellipsoidal condenser mirror for sample illumination, an objective micro zone plate and a thinned back-illuminated charge coupled device to record an x-ray image. The new, compact x-ray microscope system requires the fabrication of proper x-ray optical devices in order to obtain high-quality images. For an application-oriented microscope, the alignment procedure is fully automated via computer control through a graphic user interface. In imaging studies using our compact XM system, a gold mesh image was obtained with 45 nm resolution at x580 magnification and 1 min exposure. Images of a biological sample (Coscinodiscus oculoides) were recorded.
authors.affiliation: Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Korea.
authors.forename: Kyong Woo
authors.initials: KW
authors.lastname: Kim
authors.forename: Youngman
authors.initials: Y
authors.lastname: Kwon
authors.forename: Ki-Yong
authors.initials: KY
authors.lastname: Nam
authors.forename: Jong-Hyeok
authors.initials: JH
authors.lastname: Lim
authors.forename: Kyu-Gyum
authors.initials: KG
authors.lastname: Kim
authors.forename: Kwon Su
authors.initials: KS
authors.lastname: Chon
authors.forename: Byoung Hoon
authors.initials: BH
authors.lastname: Kim
authors.forename: Dong Eon
authors.initials: DE
authors.lastname: Kim
authors.forename: JinGon
authors.initials: J
authors.lastname: Kim
authors.forename: Byoung Nam
authors.initials: BN
authors.lastname: Ahn
authors.forename: Hyun Joon
authors.initials: HJ
authors.lastname: Shin
authors.forename: Seungyu
authors.initials: S
authors.lastname: Rah
authors.forename: Ki-Ho
authors.initials: KH
authors.lastname: Kim
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Chae
authors.forename: Dae Gab
authors.initials: DG
authors.lastname: Gweon
authors.forename: Dong Woo
authors.initials: DW
authors.lastname: Kang
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Kang
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Min
authors.forename: Kyu-Sil
authors.initials: KS
authors.lastname: Choi
authors.forename: Seong Eon
authors.initials: SE
authors.lastname: Yoon
authors.forename: Eun-A
authors.initials: EA
authors.lastname: Kim
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Namba
authors.forename: Kwon-Ha
authors.initials: KH
authors.lastname: Yoon
mesh_terms: D017377:Diatoms / Q000648:ultrastructure; D004577:Electron Probe Microanalysis / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008853:Microscopy; D046529:Microscopy, Electron, Transmission / Q000379:methods; D055096:Optical Devices; D017785:Photons; D012984:Software; D014965:X-Rays