title: Crystallization and preliminary X-ray analysis of mitochondrial presequence receptor Tom20 in complexes with a presequence from aldehyde dehydrogenase.
abstract: Most mitochondrial proteins are synthesized in the cytosol and must be imported into the mitochondria. Many mitochondrial precursor proteins have an extra leader sequence at their N-terminus called a presequence. Presequences are recognized by the Tom20 receptor protein. Based on the previously determined NMR structure of rat Tom20, a fragment corresponding to the core structure was generated. A cysteine residue was added at the C-terminus of the rat aldehyde dehydrogenase presequence to fix the presequence peptide onto the Tom20 fragment via an intermolecular disulfide bond. Two crystal forms of the complex were successfully obtained with different designs of the linker sequence which diffracted to 2.1 and 1.9 A. Crystal dehydration and subsequent annealing was essential to obtain good diffraction data for the 2.1 A crystal form.
authors.affiliation: Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Fukuoka 812-8582, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Igura
authors.forename: Toyoyuki
authors.initials: T
authors.lastname: Ose
authors.forename: Takayuki
authors.initials: T
authors.lastname: Obita
authors.forename: Chiaki
authors.initials: C
authors.lastname: Sato
authors.forename: Katsumi
authors.initials: K
authors.lastname: Maenaka
authors.forename: Toshiya
authors.initials: T
authors.lastname: Endo
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kohda
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D010455:Peptides; D021122:Protein Subunits; D011956:Receptors, Cell Surface; C489998:Tomm20 protein, rat; D000444:Aldehyde Dehydrogenase; D003545:Cysteine