Generation and characterization of a novel hematopoietic progenitor cell line with DC differentiation potential.
Studies of hematopoietic stem cell (HSC)-derived dendritic cells (DCs) are often limited by the rarity of HSC. To facilitate the study of DCs, we have generated a novel cell line (CR1) by retroviral Notch(IC) gene transfer into Sca1(+)ckit(+)lin- HSC. CR1 cells proliferated in vitro in the presence of recombinant interleukin-3. They maintained an immature progenitor cell phenotype and an intact karyotype. In the presence of granulocyte-macrophage colony-stimulating factor or Flt3L, CR1 cells differentiated into myeloid and plasmacytoid DCs, respectively. Functionally, CR1 cells were comparable to primary bone-marrow-derived DCs with respect to Toll-like-receptor-mediated maturation, cytokine release and capacity to induce effective antitumor immunity. CR1 cells thus provide an elegant new cellular tool to study DC development, function and preclinical DC-based immunotherapies.
Rathinam
C
C
Department of Pediatric Hematology and Oncology, Hannover Medical School, Hannover, Germany.
Sauer
M
M
Ghosh
A
A
Rudolph
C
C
Hegazy
A
A
Schlegelberger
B
B
Welte
K
K
Klein
C
C
D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003713:Dendritic Cells / Q000166:cytology* / Q000637:transplantation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D007167:Immunotherapy / Q000379:methods; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000494:pharmacology; D007621:Karyotyping; D007938:Leukemia / Q000276:immunology* / Q000517:prevention & control; D008565:Membrane Proteins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
D007377:Interleukin-3; D008565:Membrane Proteins; D011994:Recombinant Proteins; C084881:flt3 ligand protein; D016178:Granulocyte-Macrophage Colony-Stimulating Factor