Pre- and post-challenge immuno-haematological changes in Labeo rohita juveniles fed gelatinised or non-gelatinised carbohydrate with n-3 PUFA.
The combined effect of dietary carbohydrate type and n-3 PUFA (EPA+DHA) on pre- and post-challenge haemato-immunological responses in Labeo rohita juveniles was studied. Fish were fed for 67days with six different test diets containing either gelatinised (G) or non-gelatinised (NG) corn (43%) with three levels of n-3 PUFA (0.5%, 1.0% and 2.0%). During the pre-challenge period, significantly higher (P<0.05) NBT, serum lysozyme activity, total protein and globulin content was recorded in the NG carbohydrate fed groups. Highest NBT value was recorded in the groups fed with 1.0% n-3 PUFA, whereas the highest serum lysozyme activity (P<0.05) was recorded at either 0.5% or 2.0% n-3 PUFA fed groups in both the pre- and post-challenge period. Feeding of NG corn significantly increased the total leucocyte count, lysozyme activity, A/G ratio and decreased the total erythrocyte count, haemoglobin, serum total protein and globulin content of L. rohita juveniles during the post-challenge period. Similarly, feeding of n-3 PUFA at any level significantly increased the immunological parameters like lysozyme activity or A/G ratio, whereas total leukocyte count increased due to feeding of either 0.5% or 1.0% n-3 PUFA. The NBT and albumin values remained similar in both the pre- and post-challenge period. After challenge with Aeromonas hydrophila, the highest survival was recorded in the NG carbohydrate fed groups, whereas the lowest survival was recorded in the highest level of n-3 PUFA fed group irrespective of dietary carbohydrate type. Thus, a high level of G carbohydrate as well as n-3 PUFA is found to be immunosuppressive in L. rohita juveniles. NG carbohydrate supplemented with 1.0% n-3 PUFA is found to be optimum to enhance the immunity in L. rohita juveniles.
Misra
Sougat
S
Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
Sahu
N P
NP
Pal
A K
AK
Xavier
Biji
B
Kumar
Shivendra
S
Mukherjee
S C
SC
D016980:Aeromonas hydrophila / Q000276:immunology; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001798:Blood Proteins / Q000187:drug effects*; D002347:Carps / Q000097:blood / Q000276:immunology* / Q000382:microbiology; D004032:Diet / Q000662:veterinary; D004040:Dietary Carbohydrates / Q000008:administration & dosage* / Q000145:classification / Q000276:immunology / Q000494:pharmacology; D004906:Erythrocyte Count / Q000662:veterinary; D005227:Fatty Acids / Q000032:analysis / Q000145:classification / Q000276:immunology; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000276:immunology / Q000494:pharmacology; D005398:Fisheries; D009113:Muramidase / Q000097:blood / Q000187:drug effects*; D016897:Respiratory Burst / Q000187:drug effects* / Q000502:physiology; D016019:Survival Analysis
D001798:Blood Proteins; D004040:Dietary Carbohydrates; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D009113:Muramidase