Flow-through partial-filling affinity capillary electrophoresis using a crossreactive antibody for enantiomeric separations.
It is demonstrated that the separation of diastereoisomers and enantiomers can be accomplished by the flow-through partial-filling affinity CE using a crossreactive mAb. This approach revealed differences in the binding strength of the (+/-)-cis- and (+/-)-trans-benzo[a]pyrene tetrols with the anti-polycyclic aromatic hydrocarbon mAb and demonstrated that (+)-enantiomers are more strongly immunocomplexed than their (-)-counterparts. It is proposed that crossreactive monoclonal antibodies (i.e. mAb raised against achiral molecule and possessing limited selectivity) could be effectively utilized for specific stereoisomeric differentiation and chiral separations.
Grubor
Nenad M
NM
Ames Laboratory - USDOE, Department of Chemistry, Iowa State University, Ames, Iowa, IA, USA.
Armstrong
Daniel W
DW
Jankowiak
Ryszard
R
D000345:Affinity Labels; D000911:Antibodies, Monoclonal; D001564:Benzo(a)pyrene / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D003429:Cross Reactions; D019075:Electrophoresis, Capillary / Q000379:methods*; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D000345:Affinity Labels; D000911:Antibodies, Monoclonal; D001564:Benzo(a)pyrene; C074998:benzo(a)pyrene-7,8,9,10-tetrol