Crystallization of a truncated soluble human semicarbazide-sensitive amine oxidase.
Human semicarbazide-sensitive amine oxidase (SSAO) is a homodimeric copper-containing monoamine oxidase that occurs in both a membrane-bound and a soluble form. SSAO is also known as vascular adhesion protein-1 (VAP-1). A truncated soluble form of human SSAO (comprising residues 29-763) was expressed in human embryonic kidney 293 cells and purified to homogeneity. Tetragonal crystals were obtained and a data set extending to 2.5 A was collected. The crystals are merohedrally twinned and the estimation of the twinning fraction was complicated by pseudo-symmetry and the anisotropic character of the crystals. Using a recently developed method for twinning detection that is insensitive to phenomena such as anisotropy or pseudo-symmetry [Padilla & Yeates (2003), Acta Cryst. D59, 1124-1130], the twinning fraction was estimated to be 0.3. The structure was eventually solved by molecular replacement in space group P4(3).
Jakobsson
Emma
E
Department of Cell and Molecular Biology, Uppsala University, Biomedical Centre, Box 596, SE-751 24 Uppsala, Sweden.
Nilsson
Joakim
J
Källström
Ulla
U
Ogg
Derek
D
Kleywegt
Gerard J
GJ
D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D002460:Cell Line; D003460:Crystallization; D019281:Dimerization; D005822:Genetic Vectors; D006801:Humans; D007668:Kidney / Q000196:embryology; D008322:Mammals; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D017384:Sequence Deletion; D014162:Transfection; D014961:X-Ray Diffraction
D011994:Recombinant Proteins; D006631:Amine Oxidase (Copper-Containing)