title: Identification and characterization of a G-protein regulatory motif in WAVE1.
abstract: The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate GPR motifs. The WAVE1 GPR peptide inhibited GTPgammaS binding to purified G-protein. Endogenous Gialpha and WAVE1 coimmunoprecipitated from brain lysates. A WAVE1-G-protein complex was also observed following transfection of COS7 cells with Gialpha3 and WAVE1. The docking of Gialpha within a WAVE1 scaffolding complex may facilitate dynamic cycling and/or targeting for efficient and localized control of actin polymerization.
authors.affiliation: Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, 1901 Perdido Street, New Orleans, LA 70112, USA.
authors.forename: Kyoung Seob
authors.initials: KS
authors.lastname: Song
authors.forename: Yuri K
authors.initials: YK
authors.lastname: Peterson
authors.forename: Andrew
authors.initials: A
authors.lastname: Freidman
authors.forename: Joe B
authors.initials: JB
authors.lastname: Blumer
authors.forename: Motohiko
authors.initials: M
authors.lastname: Sato
authors.forename: S M
authors.initials: SM
authors.lastname: Lanier
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D006150:Guanine Nucleotides / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D051381:Rats; D015398:Signal Transduction; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D006150:Guanine Nucleotides; D051300:Wiskott-Aldrich Syndrome Protein Family; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D019204:GTP-Binding Proteins; D019206:GTP-Binding Protein alpha Subunits, Gi-Go