title: [Expression of caspase-8 after brain injury from fluid percussion in rats].
abstract: OBJECTIVE
  To provide the evidence of the relationship between brain injury and the time of injury.
  
  
  METHODS
  Rats were contused on brain by fluid percussion, then were killed after injury for 15 min, 30 min, 1,3,6,12 h, and 1,4,7,14 d respectively. The expression of caspase-8 were detected by immunohistochemical technology on rat brain section and the results were assessed by image analysis system in the cerebral cortex, thalamus, and hippocampus.
  
  
  RESULTS
  The expression of caspase-8 in cortex and hippocampus could be detected in 30 min after injury, increased significantly in 3h, reached apex in 1d after injury, remained 4d before decreased. In addition, the expression of caspase-8 can be detected in 1h after injury and reached apex in 1d after injury, and remained 4d then reduced.
  
  
  CONCLUSIONS
  It seems that the expression of caspase-8 should be a useful target for diagnosis of early brain injury.
authors.affiliation: West China College of Preclinical Medicine and Forensic Sciences of Sichuan University, Chendu 610041, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Chen
authors.forename: Qi-yi
authors.initials: QY
authors.lastname: Peng
authors.forename: Lei-bo
authors.initials: LB
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Liu
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D053181:Caspase 8 / Q000032:analysis*; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D016489:Head Injuries, Closed / Q000378:metabolism* / Q000473:pathology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling; D013788:Thalamus / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
chemical_list: C505528:Casp8 protein, rat; D053181:Caspase 8