Photochemical hammerhead ribozyme activation.
We report the light-activation of allosteric cis and trans acting ribozymes via decaging of a small organic molecule ligand. To achieve this effectively, we introduce an optimized N-caging group based on a nitrobenzyl core structure. This approach can potentially be employed toward a light-induced control of gene function.
Young
Douglas D
DD
North Carolina State University, Department of Chemistry, Raleigh, NC 27695-8204, USA.
Deiters
Alexander
A
D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D009690:Nucleic Acid Conformation; D010777:Photochemistry; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D014466:Ultraviolet Rays
D016337:RNA, Catalytic