title: Inoculation of mares and very young foals with EHV-1 glycoproteins D and B reduces virus shedding following respiratory challenge with EHV-1.
abstract: We have previously demonstrated that intramuscular inoculation of EHV-1 glycoprotein D (gD) and glycoprotein B (gB) produced by a recombinant baculovirus and formulated with the adjuvant Iscomatrix elicited virus-neutralizing antibody and gD- and gB-specific ELISA antibody in adult horses. In this study, 14 mares and their very young foals were inoculated with a combination of baculovirus-expressed EHV-1 gD and EHV-1 gB (EHV-1 gDBr) and challenged with a respiratory strain of EHV-1. Following experimental challenge, inoculated mares and foals shed virus in nasal secretions on significantly fewer occasions compared to uninoculated mares and foals. Uninoculated foals born from inoculated mares were no more protected against experimental challenge than uninoculated foals born from uninoculated mares. The results suggest that it is indeed possible to induce partial protection in very young foals through vaccination, and while the inoculation did not prevent infection, it did reduce the frequency of viral shedding with the potential to thereby reduce the risk and prevalence of infection in a herd situation.
authors.affiliation: Department of Biological Sciences, Macquarie University, Sydney 2109, Australia.
authors.forename: C E
authors.initials: CE
authors.lastname: Foote
authors.forename: S L
authors.initials: SL
authors.lastname: Raidal
authors.forename: G
authors.initials: G
authors.lastname: Pecenpetelovska
authors.forename: J E
authors.initials: JE
authors.lastname: Wellington
authors.forename: J M
authors.initials: JM
authors.lastname: Whalley
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000235:genetics / Q000276:immunology*; D022283:Herpesvirus Vaccines / Q000276:immunology / Q000627:therapeutic use*; D006734:Horse Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology*; D006736:Horses; D007114:Immunization / Q000379:methods / Q000662:veterinary*; D009297:Nasal Mucosa / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D022283:Herpesvirus Vaccines; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C488988:glycoprotein D, Equid herpesvirus 1