Residue analysis for halofuginone in sturgeon muscle by immunoaffinity cleanup and liquid chromatography.
A high-performance liquid chromatography (LC) method was developed for the determination of halofuginone (HFG) in sturgeon muscle. The extracted samples were cleaned up by an immunoaffinity chromatography column that was prepared by covalently coupling polyclonal antibodies against HFG to cyanogen bromide (CNBr) activated Sepharose 4B. The eluate was evaporated to dryness, and residues were determined by LC with absorbance detection at 243 nm. Recoveries of HFG from samples fortified at 20-200 microg/kg levels ranged 74.6-81.1%, with coefficients of variation of 0.7-8.6%. The detection limit was estimated to be 10 microg/kg in a 2 g sample.
Ding
Shuangyang
S
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, People's Republic of China.
Hou
Yali
Y
Wu
Ningpeng
N
Shen
Jiangzhong
J
D000818:Animals; D001492:Bass; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D003488:Cyanogen Bromide / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D009132:Muscles / Q000378:metabolism*; D010880:Piperidines; D011799:Quinazolines / Q000032:analysis*; D052999:Quinazolinones; D012685:Sepharose / Q000032:analysis; D012995:Solubility; D013696:Temperature; D013997:Time Factors; D014018:Tissue Distribution
D010880:Piperidines; D011799:Quinazolines; D052999:Quinazolinones; D012685:Sepharose; C010176:halofuginone; D003488:Cyanogen Bromide