Crystallization and preliminary X-ray crystallographic analysis of osteoclast-stimulating factor.
Osteoclast-stimulating factor increases osteoclast formation and bone resorption through a cellular signal transduction cascade, possibly by its interaction with c-Src or related family members. Crystals of human osteoclast-stimulating factor were obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant. The crystals are primitive orthorhombic and belong to P222 or a related space group, with unit-cell parameters a = 38.1, b = 54.9, c = 64.7 A.
Li
Ming
M
Laboratory of Structural Biology, Tsinghua University, Beijing 100084, People's Republic of China.
Meng
Zhaohui
Z
Xu
Yanhui
Y
Lou
Zhiyong
Z
Rao
Zihe
Z
D001483:Base Sequence; D002881:Chromosomes, Human, Pair 12; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014961:X-Ray Diffraction
D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D010455:Peptides; C408994:osteoclast stimulating factor