title: In vivo measurement of the longitudinal relaxation time of arterial blood (T1a) in the mouse using a pulsed arterial spin labeling approach.
abstract: A novel method for measuring the longitudinal relaxation time of arterial blood (T1a) is presented. Knowledge of T1a is essential for accurately quantifying cerebral perfusion using arterial spin labeling (ASL) techniques. The method is based on the flow-sensitive alternating inversion recovery (FAIR) pulsed ASL (PASL) approach. We modified the standard FAIR acquisition scheme by incorporating a global saturation pulse at the beginning of the recovery period. With this approach the FAIR tissue signal difference has a simple monoexponential dependence on the recovery time, with T1a as the time constant. Therefore, FAIR measurements performed over a range of recovery times can be fitted to a monoexponential recovery curve and T1a can be calculated directly. This eliminates many of the difficulties associated with the measurement of T1a. Experiments performed in vivo in the mouse at 2.35T produced a mean value of 1.51 s for T1a, consistent with previously published values.
authors.affiliation: RCS Unit of Biophysics, Institute of Child Health, University College London, London, UK. thomas@medphys.ucl.ac.uk
authors.forename: David L
authors.initials: DL
authors.lastname: Thomas
authors.forename: Mark F
authors.initials: MF
authors.lastname: Lythgoe
authors.forename: David G
authors.initials: DG
authors.lastname: Gadian
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Ordidge
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D002560:Cerebrovascular Circulation / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D013113:Spin Labels
chemical_list: D013113:Spin Labels