Epigallocatechin-3-gallate protects toluene diisocyanate-induced airway inflammation in a murine model of asthma.
Epigallocatechin-3-gallate (EGCG), a major form of tea catechin, has anti-allergic properties. To elucidate the anti-allergic mechanisms of EGCG, we investigated its regulation of matrix metalloproteinase (MMP-9) expression in toluene diisocyanate (TDI)-inhalation lung tissues as well as TNF-alpha and Th2 cytokine (IL-5) production in BAL fluid. Compared with untreated asthmatic mice those administrated with EGCG had significantly reduced asthmatic reaction. Also, increased reactive oxygen species (ROS) generation by TDI inhalation was diminished by administration of EGCG in BAL fluid. These results suggest that EGCG regulates inflammatory cell migration possibly by suppressing MMP-9 production and ROS generation, and indicate that EGCG may be useful as an adjuvant therapy for bronchial asthma.
Kim
Seung-Hun
SH
Department of Pediatrics, Pusan National University College of Medicine, Pusan 602-739, Republic of Korea.
Park
Hee-Ju
HJ
Lee
Chang-Min
CM
Choi
Il-Whan
IW
Moon
Dong-Oh
DO
Roh
Hwan-Jung
HJ
Lee
Hern-Ku
HK
Park
Yeong-Min
YM
D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D015848:Interleukin-5 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D051379:Mice; D017382:Reactive Oxygen Species; D012137:Respiratory System / Q000473:pathology*; D014051:Toluene 2,4-Diisocyanate / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D015848:Interleukin-5; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D014051:Toluene 2,4-Diisocyanate; D002392:Catechin; C045651:epigallocatechin gallate; D020780:Matrix Metalloproteinase 9