Aqueous two-phase systems extraction of alpha-toxin from Clostridium perfringens type A.
Two sequential half-fraction designs were applied to studying the alpha-toxin partition produced by Clostridium perfringens type A in aqueous two phase systems (ATPS), as a function of four factors: PEG molar mass and concentration, phosphate concentration and pH. The highest purification factor, yield and partition coefficient results were obtained with PEG 8000 (15%, w/w), phosphate at 20% (w/w) and pH 8.0. This system allows, in a single step, an alpha-toxin purification of 4.6-fold with final activity yield of 230% and partition coefficient of 113.9 in the PEG rich phase.
Cavalcanti
Maria Taciana Holanda
MT
Departament of Biochemical and Pharmaceutical Technology, University of São Paulo, Av. Prof. Lineu Prestes, 580, B.16. 05508-000, São Paulo, SP, Brazil.
Porto
Tatiana Souza
TS
de Barros Neto
Benicio
B
Lima-Filho
José Luiz
JL
Porto
Ana Lúcia Figueiredo
AL
Pessoa
Adalberto
A
D001427:Bacterial Toxins / Q000302:isolation & purification*; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D010738:Type C Phospholipases / Q000302:isolation & purification* / Q000378:metabolism; D014867:Water
D001427:Bacterial Toxins; D002135:Calcium-Binding Proteins; D014867:Water; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens