title: The effect of cromoglycate on time-dependent histamine and serotonin concentrations in stored blood products.
abstract: Biogenic amines, having vascular and inflammatory effects, are accepted as a potential threat for some non-hemolytic transfusion reactions. The aim of this study was to investigate time-dependent histamine/serotonin levels in stored blood products and to see whether cromoglycate has any effect on these mediators. Either for platelet or whole blood, 10-fold concentrations of cromoglycate (1 microg ml(-1), 10 microg ml(-1), 100 microg ml(-1)) with controls prepared as pairs of replicate bags collected from two healthy subjects, separately. By using enzyme immunoassay, histamine and serotonin levels were determined in platelet or blood replicates. Histamine levels increased significantly with time but serotonin remained unchanged during the storage of platelet or blood specimens. Cromoglycate had no effect on these biogenic amines except an increase of serotonin in whole blood specimens containing 100 microg ml(-1) of it. So, cromoglycate cannot protect blood products against rising levels of histamine or serotonin.
authors.affiliation: Mersin University, Faculty of Medicine, Department of Internal Medicine, Clinical Immunology Section, Mersin, Turkey. kamukon@msn.com
authors.forename: Kamuran
authors.initials: K
authors.lastname: Konca
authors.forename: E Naci
authors.initials: EN
authors.lastname: Tiftik
authors.forename: Gönül
authors.initials: G
authors.lastname: Aslan
authors.forename: Arzu
authors.initials: A
authors.lastname: Kanik
authors.forename: Atilla
authors.initials: A
authors.lastname: Yalçin
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001782:Blood Donors; D001793:Blood Preservation / Q000379:methods*; D004205:Cromolyn Sodium / Q000494:pharmacology*; D006632:Histamine / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008297:Male; D012701:Serotonin / Q000097:blood*
chemical_list: D012701:Serotonin; D006632:Histamine; D004205:Cromolyn Sodium