Crystallization of a protein using dehydration without a precipitant.
Hypoxic response protein I (HRPI) is a protein of unknown biochemical function whose expression is very strongly upregulated in response to oxygen depletion in Mycobacterium tuberculosis. Crystals have been grown from a solution of full-length HRPI by the unusual method of dehydration without the use of precipitants. The crystals produced diffract to a maximum resolution of 2.1 A and belong to space group P4(1)2(1)2 (or P4(3)2(1)2), with unit-cell parameters a = b = 79.18, c = 37.34 A.
Sharpe
Miriam L
ML
School of Biological Sciences, Centre for Molecular Biodiscovery, University of Auckland, Private Bag 92-019, Auckland 1003, New Zealand. m.sharpe@auckland.ac.nz
Baker
Edward N
EN
Lott
J Shaun
JS
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods*; D003890:Desiccation; D009169:Mycobacterium tuberculosis / Q000737:chemistry; D014961:X-Ray Diffraction
D001426:Bacterial Proteins