title: Preparation and structural determination of large oligosaccharides derived from acharan sulfate.
abstract: The structures of a series of large oligosaccharides derived from acharan sulfate were characterized. Acharan sulfate is an unusual glycosaminoglycan isolated from the giant African snail, Achatina fulica. Oligosaccharides from decasaccharide to hexadecasaccharide were enzymatically prepared using heparin lyase II and purified. Capillary electrophoresis and gel electrophoresis confirmed the purity of these oligosaccharides. Their structures, determined by ESI-MS and NMR, were consistent with the major repeating sequence in acharan sulfate, -->4)-alpha-d-GlcN(p)Ac-(1-->4)-alpha-l-IdoA(p)2S-(1-->, terminated by 4-linked alpha-d-GlcN(p)Ac residue at the reducing end and by 4,5-unsaturated pyranosyluronic acid 2-sulfate at the non-reducing end.
authors.affiliation: Department of Chemistry and Chemical Biology, Chemical and Biological Engineering, and Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Lianli
authors.initials: L
authors.lastname: Chi
authors.forename: Eva M
authors.initials: EM
authors.lastname: Munoz
authors.forename: Hyung Seok
authors.initials: HS
authors.lastname: Choi
authors.forename: Young Wan
authors.initials: YW
authors.lastname: Ha
authors.forename: Yeong Shik
authors.initials: YS
authors.lastname: Kim
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Toida
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D000818:Animals; D001438:Bacteroidaceae / Q000201:enzymology; D002240:Carbohydrate Sequence; D002384:Catalysis; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000302:isolation & purification; D019763:Heparin Lyase / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry*; D012908:Snails / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D006025:Glycosaminoglycans; D009844:Oligosaccharides; C106771:acharan sulfate; D019763:Heparin Lyase