A simple method for constructing a tagged protein.
We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.
Jo
C
C
Department of Biomedical Sciences, National Institute of Health, Seoul [122-701], Korea.
Kim
J
J
Jo
S A
SA
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005818:Genetic Engineering / Q000379:methods*; D051379:Mice; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D025842:SUMO-1 Protein