title: Genomic features of attenuated Junín virus vaccine strain candidate.
abstract: Junin virus strain Candid #1 was developed as a live attenuated vaccine for Argentine haemorrhagic fever. In this paper, we report the nucleotide sequences of L RNA of Candid #1 and examine the relationship to its more virulent ancestors Junin virus XJ#44 and XJ 13 (prototype) and other closely and distantly related arenaviruses. Comparisons of the nucleotide and amino acid sequences of L and Z genes of Candid #1 and its progenitor strains revealed twelve point mutations in the L polypeptide that are unique to the vaccine strain. These changes could be provisionally associated with the attenuated phenotype. In contrast, Z ORF was completely conserved among all strains.
authors.affiliation: LIGBCM, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Rogue Saenz Peña 180, B1876BXD, Bernal, Buenos Aires, Argentina.
authors.forename: Sandra Elizabeth
authors.initials: SE
authors.lastname: Goñi
authors.forename: Javier Alonso
authors.initials: JA
authors.lastname: Iserte
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Ambrosio
authors.forename: Victor
authors.initials: V
authors.lastname: Romanowski
authors.forename: Pablo Daniel
authors.initials: PD
authors.lastname: Ghiringhelli
authors.forename: Mario Enrique
authors.initials: ME
authors.lastname: Lozano
mesh_terms: D000818:Animals; D001117:Arenaviridae Infections / Q000276:immunology / Q000517:prevention & control; D016679:Genome, Viral; D006168:Guinea Pigs; D006801:Humans; D018051:Junin virus / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D017354:Point Mutation; D012367:RNA, Viral / Q000235:genetics; D013045:Species Specificity; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
chemical_list: D012367:RNA, Viral; D014613:Vaccines, Attenuated; D014764:Viral Proteins; D014765:Viral Vaccines