Attenuation of neutrophil infiltration and proinflammatory cytokines by Cissus quadrangularis: a possible prevention against gastric ulcerogenesis.
Reactive oxygen species, neutrophil infiltration and proinflammatory cytokines play an important role in the pathogenesis of gastric ulcers caused by aspirin. The present study demonstrates the healing effect of Cissus quadrangularis extract (CQE) through inhibitory action on generation of lipid peroxidation, proinflammatory cytokines and neutrophil infiltration. The concentration ofmalondialdehye (MDA), protein carbonyl content, conjugated dienes, mucosal (SH) sulphydryls, uric acid, tumor necrosis factor-alpha (TNF-alpha), and activities of myeloperoxidase (MPO), xanthine oxidase (XO) and antioxidative enzymes were determined in the gastric mucosa. Administration of CQE significantly attenuated the gastric lesions induced by aspirin and this was accompanied by the rise in uric acid, antioxidative enzymes, SH groups, and a significant decrease in lipid peroxidase, TNF-alpha, MPO and XO activities. These findings suggest that the significant gastroprotective activity could be mediated by the antioxidant activity as well as by the attenuation of oxidative mechanism and proinflammatory cytokines.
Jainu
Mallika
M
Department of Biochemistry, University of Madras, Guindy Campus, Chennai, Tamil Nadu, India.
Shyamala Devi
Chennam Srinivasulu
CS
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D001241:Aspirin; D032422:Cissus; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D020556:Neutrophil Infiltration / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D016207:Cytokines; D010936:Plant Extracts; D020011:Protective Agents; D001241:Aspirin