Novel conducting polymer electrolyte biosensor based on poly(1-vinyl imidazole) and poly(acrylic acid) networks.
Biosensor construction and characterization studies of poly(acrylic acid) (PAA) and poly(1-vinyl imidazole) (PVI) complex systems have been carried out. The biosensors were prepared by mixing PAA with PVI at several stoichiometric ratios, x (molar ratio of the monomer repeat units). The enzyme, invertase, was entrapped in the PAA/PVA interpenetrating polymer networks during complexation. Modifications were made on the PAA/PVI conducting polymer electrolyte matrixes to improve the stability and performance of the polymer electrolyte-based enzyme biosensor. The maximum reaction rate (V(max)) and Michaelis-Menten constant (K(m)) were investigated for the immobilized invertase. The temperature and pH optimization, operational stability, and shelf life of the polymer electrolyte biosensor were also examined.
Arslan
Ahu
A
Department of Chemistry, Middle East Technical University, 06531 Ankara, Turkey.
Kiralp
Senem
S
Toppare
Levent
L
Bozkurt
Ayhan
A
D000180:Acrylic Resins / Q000737:chemistry*; D015374:Biosensing Techniques; D004573:Electrolytes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry*; D007700:Kinetics; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D043324:beta-Fructofuranosidase / Q000737:chemistry
D000180:Acrylic Resins; D004573:Electrolytes; D007093:Imidazoles; D011108:Polymers; D011145:Polyvinyls; C072974:polyvinylimidazole; C006903:carbopol 940; D043324:beta-Fructofuranosidase