A comparative in vitro study of the toxic potency of five inorganic lead compounds on a rat liver epithelial cell line (REL).
Relative insolubility of inorganic Pb compounds is one of the major problems in the evaluation of the toxicological profile of this metal. Different characteristics of Pb-containing solutions may, in fact, alter the biological properties of Pb compounds and influence their toxic potency. To investigate these aspects, we used selected experimental conditions to evaluate and compare the specific biological effects of five inorganic Pb compounds (soluble salts and oxide) on the viability and proliferation rate of a rat liver-derived cell line (REL cells). The study was performed according to classical toxicological criteria (dose- and time-response, reversibility/transience of the effect). Each Pb compound was accurately solubilised and the quantification of the real concentration of Pb(II) ions was performed either on the culture media used for each treatment, or on the extracts of exposed cells. Our study shows that four, out of the five Pb compounds we tested, induce the same dose- and time-related anti-proliferative effects on REL cells, being these effects also reversible, transient and directly related to the intracellular content of the metal. Since the intracellular concentration of the metal and, consequently, its biological effects on REL cells, directly depends on the bioavailability of the Pb(II) cation present in the treatment solutions, our results indicate that, in the experimental procedures aimed to assess the toxic potency of this metal, the solubility of each Pb compound should be carefully evaluated and taken into account.
Aleo
M F
MF
Unit of Biochemistry, School of Medicine, University of Brescia, Department of Biomedical Sciences and Biotechnologies, viale Europa 11, 25123 Brescia, Italy. aleo@med.unibs.it
Bettoni
F
F
Boniotti
J
J
Morandini
F
F
Giuliani
R
R
Steimberg
N
N
Apostoli
P
P
Mazzoleni
G
G
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects; D007854:Lead / Q000378:metabolism / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D051381:Rats
D007854:Lead