title: Crystallization and preliminary X-ray diffraction studies of NP24-I, an isoform of a thaumatin-like protein from ripe tomato fruits.
abstract: NP24 is a 24 kDa (207-amino-acid) antifungal thaumatin-like protein (TLP) found in tomato fruits. An isoform of the protein, NP24-I, is reported to play a possible role in ripening of the fruit in addition to its antifungal properties. The protein has been isolated and purified and crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the tetragonal space group P4(3), with unit-cell parameters a = b = 61.01, c = 62.90 A and one molecule per asymmetric unit. X-ray diffraction data were processed to a resolution of 2.45 A and the structure was solved by molecular replacement.
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata 700064, India.
authors.forename: Raka
authors.initials: R
authors.lastname: Ghosh
authors.forename: Chandana
authors.initials: C
authors.lastname: Chakrabarti
mesh_terms: D003460:Crystallization; D018551:Solanum lycopersicum / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D010940:Plant Proteins; D020033:Protein Isoforms; C003427:thaumatin protein, plant