title: IFN-gamma: regulation of nitric oxide in the P. aeruginosa-infected cornea.
abstract: OBJECTIVE
  BALB/c mice are resistant to Pseudomonas aeruginosa (P. aeruginosa) keratitis and bacterial killing/stasis requires nitric oxide (NO). NO regulation in the cornea is unknown and was tested in this model.
  
  
  METHODS
  Nitrite detection, IFN-gamma-knockout mice, TNF-alpha neutralization, ELISA, aminoguanidine (AG) treatment, MPO, and plate counts were done.
  
  
  RESULTS
  Evidence shows (i) without IFN-gamma, nitrite levels are elevated, (ii) neutralization of TNF-alpha does not change nitrite levels, and (iii) absence of IFN-alpha and reduced NO synergistically increases disease progress and upregulates pro-inflammatory cytokines, PMN number, and bacterial load.
  
  
  CONCLUSIONS
  IFN-gamma regulates NO levels, and synergistic interaction between the two regulates disease outcome in resistant mice.
authors.affiliation: Department of Anatomy & Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: Sharon A
authors.initials: SA
authors.lastname: McClellan
authors.forename: Shahrzad
authors.initials: S
authors.lastname: Lighvani
authors.forename: Linda D
authors.initials: LD
authors.lastname: Hazlett
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D003315:Cornea / Q000378:metabolism / Q000382:microbiology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D015818:Eye Infections, Bacterial / Q000378:metabolism* / Q000382:microbiology; D005260:Female; D006146:Guanidines / Q000494:pharmacology; D007371:Interferon-gamma / Q000502:physiology*; D007634:Keratitis / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009573:Nitrites / Q000378:metabolism; D011552:Pseudomonas Infections / Q000378:metabolism* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*
chemical_list: D016207:Cytokines; D004791:Enzyme Inhibitors; D006146:Guanidines; D009573:Nitrites; D009569:Nitric Oxide; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; C004479:pimagedine