A multidimensional overpressured layer chromatographic method for the characterization of tetrazine libraries.
The recent combinatorial approach in synthetic organic chemistry started a new age in drug discovery. The generation of compound libraries in combination with high-throughput screening has become the method of choice for the production of new pharmacological leads for chemical optimization. Characterization and separation of such pool of compounds have been lagging behind the synthetic and screening methodologies. Overpressured layer chromatography (OPLC) is an instrumentalized planar liquid chromatographic technique associated with the use of optimized layers prepared from particles of narrow particle size distribution and small diameter. On one hand, uni-directional OPLC allows the simultaneous separation of large number of samples in minutes. On the other hand, two-dimensional OPLC offers multidimensional separation on a single layer. This paper shows the complete multidimensional separation of a tetrazine library prepared by parallel combinatorial synthesis. In general, this approach may become the method of choice for the characterization of compound libraries.
Gombosuren
Naran
N
Eötvös Loránd University, Department of Organic Chemistry, Pázmány sétány 1/A, H-1117 Budapest, Hungary.
Novák
Zoltán
Z
Kotschy
András
A
Mincsovics
Emil
E
Dibó
Gábor
G
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006575:Heterocyclic Compounds, 3-Ring / Q000032:analysis / Q000138:chemical synthesis* / Q000737:chemistry
D006575:Heterocyclic Compounds, 3-Ring