Bone-related gene profiles in developing calvaria.
Generating a comprehensive understanding of osteogenesis-related gene profiles is very important in the development of new treatments for osteopenic conditions. Developing calvaria undergoes a typical intramembranous bone-forming process. To identify genes associated with osteoblast differentiation, we isolated total RNAs from parietal bones, that represent active osteoblasts, and sutural mesenchyme, that represents osteoprogenitor cells, and comprehensively analyzed their gene expression profiles using an oligo-based Affymetrix microarray chip containing 22,690 probes. About 2100 genes with "Present" calls had more than 2-fold higher expression in bone compared to sutures while 73 of these genes had more than 8-fold expression. Some of these genes are already known to be bone-related biomarkers: VitD receptor, bone sialoprotein, osteocalcin, osteopontin, MMP13, etc. Eight genes were selected and subjected to confirmation by quantitative real-time RT-PCR analyses. All the genes tested showed higher expression in bones, ranging from 5- to 140-fold. Several of these genes are ESTs while others are already known but their functions in osteogenesis were not previously known. Most genes of the BMP and FGF families probed in the Genechip analysis were more highly expressed in bone tissues compared to suture. All differentially-expressed Runx and Dlx family genes also showed higher expression in bone. These results imply that our data is valid and can be used as a good standard for the mining of osteogenesis-related genes.
Cho
Je-Yoel
JY
Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu, Republic of Korea.
Lee
Won-Bong
WB
Kim
Hyun-Jung
HJ
Mi Woo
Kyung
K
Baek
Jeong-Hwa
JH
Choi
Je-Yong
JY
Hur
Cheol-Gu
CG
Ryoo
Hyun-Mo
HM
D000818:Animals; D001846:Bone Development / Q000235:genetics*; D002454:Cell Differentiation; D003393:Cranial Sutures / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D050437:Genes, Developmental / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D046228:Microarray Analysis; D010006:Osteoblasts / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012886:Skull / Q000196:embryology* / Q000378:metabolism*
D012333:RNA, Messenger