title: Post mortem parasitological evaluation of dogs seroreactive for Leishmania from Rio de Janeiro, Brazil.
abstract: A parasitological study was conducted on 66 dogs seroreactive for Leishmania captured as a control measure of visceral leishmaniasis in the State of Rio de Janeiro, Brazil. Biological samples from different anatomical sites were collected during autopsy of the animals and cultured on biphasic medium (NNN/Schneider). The Leishmania isolates were characterized by isoenzyme electrophoresis. Leishmania was isolated from 80.3% of the animals: 12 animals with Leishmania (Viannia) braziliensis isolated exclusively from cutaneous lesions, 39 with L. (L.) chagasi isolated from different sites in the same animal, and 2 with simultaneous isolation of L. (V.) braziliensis from cutaneous lesions and L. (L.) chagasi from different sites. Isolation in culture revealed the absence of Leishmania parasites in 13 animals. The results obtained confirm the existence of mixed infections in dogs in Rio de Janeiro and indicate the need to complement the investigation of seroreactive dogs using methods for the parasitological diagnosis and identification of Leishmania species.
authors.affiliation: Serviço de Parasitologia, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil. fmadeira@ipec.fiocruz.br
authors.forename: Maria
authors.initials: M
authors.lastname: de Fátima Madeira
authors.forename: Armando
authors.initials: A
authors.lastname: de O Schubach
authors.forename: Tânia M P
authors.initials: TM
authors.lastname: Schubach
authors.forename: Sandro A
authors.initials: SA
authors.lastname: Pereira
authors.forename: Fabiano B
authors.initials: FB
authors.lastname: Figueiredo
authors.forename: Cibele
authors.initials: C
authors.lastname: Baptista
authors.forename: Cristianni A
authors.initials: CA
authors.lastname: Leal
authors.forename: Cíntia X
authors.initials: CX
authors.lastname: Melo
authors.forename: Eliame M
authors.initials: EM
authors.lastname: Confort
authors.forename: Mauro C A
authors.initials: MC
authors.lastname: Marzochi
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001938:Brazil; D002478:Cells, Cultured; D004283:Dog Diseases / Q000276:immunology / Q000469:parasitology* / Q000473:pathology; D004285:Dogs; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007527:Isoenzymes / Q000032:analysis; D007891:Leishmania / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D007892:Leishmania braziliensis / Q000302:isolation & purification; D007896:Leishmaniasis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*
chemical_list: D000913:Antibodies, Protozoan; D007527:Isoenzymes