F0F1-ATPase as biosensor to detect single virus.
F(0)F(1)-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody-antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F(0)F(1)-ATPase, which was indicated by F1300, was directly observed by a fluorescence microscope. The results demonstrate that the biosensor loading of virus particles has remarkable signal-to-noise ratio (3.8:1) compared to its control at single molecular level, and will be convenient, quick, and even super-sensitive for detecting virus particles.
Liu
Xiaolong
X
The National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Zhang
Yun
Y
Yue
Jiachang
J
Jiang
Peidong
P
Zhang
Zhenxi
Z
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D009980:Influenza A virus / Q000276:immunology* / Q000302:isolation & purification*; D008856:Microscopy, Fluorescence / Q000379:methods; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D006180:Proton-Translocating ATPases