In vitro models for studying human uterine and placental macrophages.
Human monocytes and macrophages, which are also called mononuclear phagocytes, represent a major arm of the innate immune system. These cells not only protect against infection but are also central to tissue remodeling and production of chemokines, cytokines, and growth factors. Tissue macrophages reside in the human placenta and uterine decidua throughout pregnancy, where they comprise part of the host defense network and facilitate placental and extraembryonic development. The purpose of this chapter is to describe methods for establishing useful models of human uteroplacental macrophages: (1) differentiated U937 myelomonocytic cells, (2) peripheral blood monocytes, (3) peripheral blood monocyte-derived macrophages, (4) decidual macrophages, and (5) placental macrophages.
McIntire
Ramsey H
RH
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS, USA.
Petroff
Margaret G
MG
Phillips
Teresa A
TA
Hunt
Joan S
JS
D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D003470:Culture Media; D004717:Endometrium / Q000276:immunology; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D009000:Monocytes / Q000166:cytology; D010920:Placenta / Q000276:immunology*; D011247:Pregnancy; D020298:U937 Cells / Q000502:physiology; D014599:Uterus / Q000276:immunology*
D003470:Culture Media