title: Reactivity of antibodies from syphilis patients to a protein array representing the Treponema pallidum proteome.
abstract: To identify antigens important in the human immune response to syphilis, the serum antibody reactivity of syphilitic patients was examined with 908 of the 1,039 proteins in the proteome of Treponema pallidum subsp. pallidum using a protein array enzyme-linked immunosorbent assay. Thirty-four proteins exhibited significant reactivity when assayed with human sera from patients in the early latent stage of syphilis. A subset of antigens identified were further scrutinized for antibody reactivity at primary, secondary, and latent disease stages, and the results demonstrate that the humoral immune response to individual T. pallidum proteins develops at different rates during the time course of infection.
authors.affiliation: Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
authors.forename: Mary Beth
authors.initials: MB
authors.lastname: Brinkman
authors.forename: Matthew
authors.initials: M
authors.lastname: McKevitt
authors.forename: Melanie
authors.initials: M
authors.lastname: McLoughlin
authors.forename: Carla
authors.initials: C
authors.lastname: Perez
authors.forename: Jerrilyn
authors.initials: J
authors.lastname: Howell
authors.forename: George M
authors.initials: GM
authors.lastname: Weinstock
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Norris
authors.forename: Timothy
authors.initials: T
authors.lastname: Palzkill
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome; D013587:Syphilis / Q000276:immunology*; D014210:Treponema pallidum / Q000235:genetics* / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D020543:Proteome