Immunomodulatory effects of IL-12 secreted by Lactococcus lactis on Th1/Th2 balance in ovalbumin (OVA)-induced asthma model mice.
Asthma is a chronic lung disease characterized by allergen-induced airway inflammation and orchestrated by Th2 cells. Interleukin-12, a Th1-promoting cytokine, is capable of inhibit the Th2-driven allergen-induced airway changes and therefore considered as an attractive molecule to treat asthma. Recent epidemiological and clinical studies suggest a possible role of Lactococcus lactis in the prevention of allergic diseases. In this study, we evaluated the immunomodulatory effects of live L. lactis secreting a biologically active form of IL-12 (LL-IL12) in a mouse model of ovalbumin (OVA)-induced asthma. Intranasal mice administration with LL-IL12 resulted in a shift Th2 to Th1 with elevated IFN-gamma and decreased IL-4 levels. In addition, a profound decrease in airway hyper-responsiveness and pulmonary inflammation was also observed in mice administered with LL-IL12. These promising preclinical results suggest the feasibility of this approach to be used in the treatment of asthma.
Wu
Changyi
C
Department of Anesthesiology, First Affiliated Hospital, China Medical University, Shenyang.
Yang
Guang
G
Bermúdez-Humarán
Luis G
LG
Pang
Qingfeng
Q
Zeng
Yinming
Y
Wang
Junke
J
Gao
Xinyue
X
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000096:biosynthesis / Q000494:pharmacology*; D000281:Administration, Intranasal; D000818:Animals; D018927:Anti-Asthmatic Agents; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D004342:Drug Hypersensitivity / Q000473:pathology; D004804:Eosinophils / Q000502:physiology; D018664:Interleukin-12 / Q000008:administration & dosage / Q000096:biosynthesis / Q000494:pharmacology*; D013294:Lactococcus lactis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D018417:Th1 Cells / Q000187:drug effects*; D018418:Th2 Cells / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D018927:Anti-Asthmatic Agents; D016207:Cytokines; D018664:Interleukin-12; D010047:Ovalbumin