Polymorphonuclear neutrophils deliver activation signals and antigenic molecules to dendritic cells: a new link between leukocytes upstream of T lymphocytes.
Polymorphonuclear neutrophils (PMNs) are rapidly recruited to tissues upon injury or infection. There, they can encounter local and/or recruited immature dendritic cells (iDCs), a colocalization that could promote at least transient interactions and mutually influence the two leukocyte populations. Using human live blood PMNs and monocyte-derived iDCs, we examined if these leukocytes actually interacted and whether this influenced DC function. Indeed, coculture with live but not apoptotic PMNs led to up-regulation of membrane CD40, CD86, and human leukocyte antigen (HLA)-DR on DCs. Whereas CD40 up-regulation was dependent on soluble factors released by PMNs, as determined in cultures conducted in different chambers, cell contact was necessary for CD86 and HLA-DR up-regulation, a process that was inhibited by anti-CD18 antibodies, indicating that CD18 ligation was required. We also found that via a cell contact-dependent mechanism, DCs acquired Candida albicans-derived antigens from live as well as from apoptotic PMNs and could thus elicit antigen-specific T lymphocyte responses. Altogether, our data demonstrate the occurrence of cross-talk between human PMNs and DCs and provide new insights into the immune processes occurring upstream of the interactions between DCs and T lymphocytes.
Megiovanni
Anna Maria
AM
Unité Mixte de Recherche 7151 Centre National de la Recherche Scientifique-Université Paris, France.
Sanchez
Françoise
F
Robledo-Sarmiento
Macarena
M
Morel
Céline
C
Gluckman
Jean Claude
JC
Boudaly
Sarah
S
D017951:Antigen Presentation / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D051940:B7-2 Antigen / Q000276:immunology; D018821:CD18 Antigens / Q000276:immunology; D019013:CD40 Antigens / Q000276:immunology; D002176:Candida albicans / Q000276:immunology; D002177:Candidiasis / Q000276:immunology; D002450:Cell Communication / Q000276:immunology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D016130:Immunophenotyping; D008026:Ligation; D008213:Lymphocyte Activation / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D015854:Up-Regulation / Q000276:immunology
D000954:Antigens, Surface; D051940:B7-2 Antigen; D018821:CD18 Antigens; D019013:CD40 Antigens; D006684:HLA-DR Antigens