Mechanism of Cl- selection by a glutamate-gated chloride (GluCl) receptor revealed through mutations in the selectivity filter.
To learn about the mechanism of ion charge selectivity by invertebrate glutamate-gated chloride (GluCl) channels, we swapped segments between the GluClbeta receptor of Caenorhabditis elegans and the vertebrate cationic alpha7-acetylcholine receptor and monitored anionic/cationic permeability ratios. Complete conversion of the ion charge selectivity in a set of receptor microchimeras indicates that the selectivity filter of the GluClbeta receptor is created by a sequence connecting the first with the second transmembrane segments. A single substitution of a negatively charged residue within this sequence converted the selectivity of the GluClbeta receptor's pore from anionic to cationic. Unexpectedly, elimination of the charge of each basic residue of the selectivity filter, one at a time or concomitantly, moderately reduced the P(Cl)/P(Na) ratios, but the GluClbeta receptor's mutants retained high capacity to select Cl(-) over Na(+). These results indicate that, unlike the proposed case of anionic Gly- and gamma-aminobutyric acid-gated ion channels, positively charged residues do not play the key role in the selection of ionic charge by the GluClbeta receptor. Taken together with measurements of the effective open pore diameter and with structural modeling, the study presented here collectively indicates that in the most constricted part of the open GluClbeta receptor's channel, Cl(-) interacts with backbone amides, where it undergoes partial dehydration necessary for traversing the pore.
Sunesen
Morten
M
Unit of Receptors and Cognition, URA 2182 CNRS, Pasteur Institute, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France.
de Carvalho
Lia Prado
LP
Dufresne
Virginie
V
Grailhe
Regis
R
Savatier-Duclert
Nathalie
N
Gibor
Gilad
G
Peretz
Asher
A
Attali
Bernard
B
Changeux
Jean-Pierre
JP
Paas
Yoav
Y
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans; D018118:Chloride Channels / Q000737:chemistry* / Q000235:genetics*; D002712:Chlorides / Q000737:chemistry*; D004594:Electrophysiology; D005971:Glutamates / Q000737:chemistry*; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
D018118:Chloride Channels; D002712:Chlorides; D005971:Glutamates; D011993:Recombinant Fusion Proteins; C109777:glutamate-gated chloride channels