title: A highly thermostable and alkaline amylase from a Bacillus sp. PN5.
abstract: A highly thermostable alkaline amylase producing Bacillus sp. PN5 was isolated from soil, which yielded 65.23 U mL(-1) of amylase in medium containing (%) 0.6 starch, 0.5 peptone and 0.3 yeast extract at 60 degrees C, pH 7.0 after 60 h of incubation. Maximum amylase activity was at pH 10.0 and 90 degrees C. The enzyme retained 80% activity after 1 h at pH 10.0. It exhibited 65% activity at 105 degrees C and had 100% stability in the temperature range between 80 and 100 degrees C for 1 h. In addition, there was 86.36% stability after 1-h incubation with sodium dodecylsulphate. These properties indicated possible use of this amylase in starch saccharification and detergent formulation.
authors.affiliation: Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India. rksmicro@hotmail.com
authors.forename: Rajendra Kumar
authors.initials: RK
authors.lastname: Saxena
authors.forename: Kakoli
authors.initials: K
authors.lastname: Dutt
authors.forename: Lata
authors.initials: L
authors.lastname: Agarwal
authors.forename: Preeti
authors.initials: P
authors.lastname: Nayyar
mesh_terms: D000681:Amylases / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D000681:Amylases