[Effects and mechanism of pentoxifylline on the expression of intercellular adhesion molecule-1 in the rat cardiac myocytes after lipopolysaccharide challenge].
OBJECTIVE
To investigate the effects of pentoxifylline (PTX) on the expression of intercellular adhesion molecule-1 (ICAM-1) of rat cardiac myocytes after lipopolysaccharide (LPS) challenge in vitro, and to evaluate the protective effect of PTX on cardiac myocytes and its mechanism.


METHODS
Cultured cardiac myocytes were randomly divided into three groups: control group, LPS group and PTX protection group. No drug was given in the control group. In LPS group, a part of cardiac myocytes were stimulated with 100 microg/L of LPS for 2, 4, 6, 8 hours, respectively, and the others were stimulated with 10, 50, 100 microg/L LPS, respectively, for 6 hours. Expression of ICAM-1, nuclear factor-KappaB (NF-KappaB) p65 and NF-KappaB inhibitor-alpha (IKappaB-alpha) in cardiac myocytes was respectively measured with enzyme-linked immunoadsorbent assay (ELISA), immunohistochemistry, and Western blot. The expression of ICAM-1 in cardiac myocyte was also determined after the addition of 50, 100, 200 mg/L of PTX before LPS challenge.


RESULTS
The intensity of expression of ICAM-1 was highly dependent on the stimulation of LPS in dose depending and time-depending manner. The stimulation quickly resulted in activation of NF-KappaB p65 to translocate into nuclei. This process peaked at 1 hours and then declined. The expression of IKappaB-alpha was depressed at first, then elevated. With the addition of PTX, the expression of ICAM-1 and NF-KappaB p65 was depressed, and the expression of IKappaB-alpha was elevated.


CONCLUSIONS
PTX may have a protective effect on the cardiac myocytes against LPS injury through inhibiting the pathway of NF-KappaB, which regulates the production of ICAM-1.
Gu
Jian-jun
JJ
Department of Intensive Care Unit, The Second Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China. gujianjundt@163.com
Sun
Hua
H
D000818:Animals; D002478:Cells, Cultured; D020672:I-kappa B Proteins / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism; D010431:Pentoxifylline / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D020672:I-kappa B Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; C000606206:Nfkbia protein, rat; D018799:Intercellular Adhesion Molecule-1; D000072000:NF-KappaB Inhibitor alpha; D010431:Pentoxifylline