title: Rapid preparation of stable isotope labeled peptides that bind to target proteins by a phage library system.
abstract: We have developed a system for directly isolating foreign peptides displayed on the N-terminus of the major coat protein of bacteriophage M13. The phage particle in this system is formed as a mixture of wild type and modified coat proteins. The N-terminal segment of the modified coat protein was mutated for chemical cleavage, in order to obtain the displayed peptide from the major coat protein. Using 13C, 15N- labeled medium, we introduced stable isotopes, 13C and/or 15N, into the coat proteins. The NMR spectra for the cleaved peptides from the phage particles could be recorded within a few days after the selection of the phage clone.
authors.affiliation: Japan Biological Information Research Center (JBIRC), Japan Biological Informatics Consortium (JBIC), Tokyo, 135-0064, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Mizukoshi
authors.forename: Hideo
authors.initials: H
authors.lastname: Takahashi
authors.forename: Ichio
authors.initials: I
authors.lastname: Shimada
mesh_terms: D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002247:Carbon Isotopes / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D019151:Peptide Library; D010455:Peptides