title: Multiple-axis tomography: applications to basal bodies from Paramecium tetraurelia.
abstract: BACKGROUND
  Transmission electron tomography is becoming a powerful tool for studying subcellular components of cells. Classical approaches for electron tomography consist of recording images along a single-tilt axis. This approach is being improved by dual-axis reconstructions and/or high-tilt devices (tilt angle>+/-60 degrees) on microscopes to compensate part of the information loss due to the 'missing wedge' phenomena.
  
  
  RESULTS
  In the present work we have evaluated the extension of the dual-axis technique to a multiple-axis approach, and we demonstrate a freely available plug-in for the Java-based freeware image-analysis software ImageJ. Our results from phantom and experimental data sets from Paramecium tetraurelia epon-embedded sections have shown that multiple-axis tomography achieves results equivalent to those obtained by dual-axis approach without the requirement for high-tilt devices.
  
  
  CONCLUSIONS
  This new approach allows performance of high-resolution tomography, avoiding the need for high-tilt devices, and therefore will increase the access of electron tomography to a larger community.
authors.affiliation: INSERM U759, Imagerie intégrative, 91405 Orsay, France, and Institut Curie, Centre de Recherche, Laboratoire Raymond Latarjet, Centre Universitaire d'Orsay, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Messaoudi
authors.forename: Nicole Garreau
authors.initials: NG
authors.lastname: de Loubresse
authors.forename: Thomas
authors.initials: T
authors.lastname: Boudier
authors.forename: Pascale
authors.initials: P
authors.lastname: Dupuis-Williams
authors.forename: Sergio
authors.initials: S
authors.lastname: Marco
mesh_terms: D000465:Algorithms; D000818:Animals; D002923:Cilia / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron / Q000379:methods*; D008870:Microtubules / Q000648:ultrastructure; D016805:Paramecium tetraurelia / Q000166:cytology / Q000648:ultrastructure*; D014054:Tomography / Q000379:methods*