Assembling and cloning genes for fusion proteins using reverse transcription one-step overlap extension PCR method.
Nagy
Zsolt B
ZB
Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, Szeged H-6701, Hungary.
Varga-Orvos
Zoltán
Z
Szakál
Barnabás
B
Tamás
László
L
Puskás
László G
LG
D000818:Animals; D037421:Anthozoa / Q000737:chemistry; D003001:Cloning, Molecular / Q000379:methods; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014162:Transfection; D051844:alpha-Synuclein / Q000235:genetics; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D051844:alpha-Synuclein; D000097573:Red Fluorescent Protein