title: Laser autofocusing system for high-resolution cell biological imaging.
abstract: Automated acquisition of high resolution, light microscope images of cells is becoming a common requirement in modern proteomic and cellomic research. A prerequisite for such microscopy is fine focus tuning, commonly optimized by multiple exposures, followed by image sharpness analysis. We describe here an extremely fast and accurate laser autofocusing system with distinct advantages for large-scale cell-based screening.
authors.affiliation: Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Liron
authors.forename: Y
authors.initials: Y
authors.lastname: Paran
authors.forename: N G
authors.initials: NG
authors.lastname: Zatorsky
authors.forename: B
authors.initials: B
authors.lastname: Geiger
authors.forename: Z
authors.initials: Z
authors.lastname: Kam
mesh_terms: D001331:Automation; D002460:Cell Line; D048430:Cell Shape; D004867:Equipment Design; D007834:Lasers; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D055095:Optics and Photonics / Q000295:instrumentation