title: Bioactive dibenzylbutyrolactone and dibenzylbutanediol lignans from Peperomia duclouxii.
abstract: Six new dibenzylbutyrolactone (6-11) and two new dibenzylbutanediol lignans (12, 13) were obtained from Peperomia duclouxii. The structures were elucidated mainly by the analysis of NMR and MS data. The anticancer activity against a normal (WI-38) and a simian virus 40-transformed human lung fibroblast cell (VA-13) and a hepatoma G2 cell (HepG2) and the MDR reversal activity of the isolated compounds were examined. Compound 7 showed moderate inhibitory activity against VA-13 and HepG2 with IC(50) values of 23.2 and 26.4 microM, respectively. Compound 2 inhibited the growth of HepG2 cells with an IC(50) of 42.8 microM. Compounds 2 and 13 exhibited stronger MDR reversal activity than verapamil, at 25 and 2.5 microg/mL, respectively, and 4, 5, and 7 showed comparable activity with verapamil, at 25, 25, and 2.5 microg/mL, respectively.
authors.affiliation: The National Center for Drug Screening, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China. nali9898@hotmail.com
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Jian-Lin
authors.initials: JL
authors.lastname: Wu
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Sakai
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Wang
authors.forename: Saori
authors.initials: S
authors.lastname: Kakuta
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Furuya
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tomida
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuruo
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ando
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D036941:Peperomia / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D017705:Lignans; D014700:Verapamil