title: Colonization of mycoplasma in the upper respiratory tract of AIDS patients with pulmonary symptoms in Chennai, India.
abstract: OBJECTIVE
  Mycoplasmas have been implicated in causing minor to severe respiratory infections in man. Mycoplasmas are considered to act as cofactors in patients with AIDS. A preliminary study was conducted to isolate mycoplasmas from sputum specimens of AIDS patients and non-HIV patients with underlying pulmonary symptoms and signs.
  
  
  METHODS
  A total of 130 sputum samples (100 from AIDS patients and 30 from non-HIV) were cultured on standard pleuropneumonia-like organisms (PPLO) glucose agar up to 3 wk. The plates were examined for the presence of fried-egg colonies characteristic of Mycoplasma. Subsequently the plates were stained using Diene's stain. Sputum specimens from the AIDS patients were also screened for other bacterial pathogens.
  
  
  RESULTS
  Mycoplasmas were detected from 36 (36%) of the AIDS patients and only 5 (16.6%) of the non HIV control individuals with underlying pulmonary symptoms. Data on the detection rates of other microorganisms from the AIDS cases were also analysed.
  
  
  CONCLUSIONS
  This preliminary study provided supportive evidence that mycoplasma colonized in upper respiratory tract of individuals with AIDS to a larger extent than that of the non HIV subjects with pulmonary symptoms. Further studies need to be done to characterize mycoplasma isolates to species level.
authors.affiliation: Department of Microbiology, Dr ALM Postgraduate Institute of Basic Medical Sciences, University of Madras, Chennai, India.
authors.forename: E M
authors.initials: EM
authors.lastname: Shankar
authors.forename: S
authors.initials: S
authors.lastname: Rajasekaran
authors.forename: Usha Anand
authors.initials: UA
authors.lastname: Rao
authors.forename: P
authors.initials: P
authors.lastname: Paramesh
authors.forename: R
authors.initials: R
authors.lastname: Krishnakumar
authors.forename: R
authors.initials: R
authors.lastname: Rajan
authors.forename: H
authors.initials: H
authors.lastname: Kownhar
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D009174:Mycoplasma / Q000302:isolation & purification* / Q000472:pathogenicity; D009175:Mycoplasma Infections / Q000150:complications* / Q000382:microbiology; D012141:Respiratory Tract Infections / Q000150:complications* / Q000382:microbiology; D013183:Sputum / Q000382:microbiology