Crystallization and preliminary crystallographic study of the yeast Malassezia sympodialis allergen Mala s 1.
The opportunistic yeast Malassezia sympodialis can act as an allergen and elicit specific IgE- and T-cell reactivity in patients with atopic eczema. The first identified major allergen from M. sympodialis, Mala s 1, is present on the cell surface of the yeast. Recombinant Mala s 1 was expressed in Escherichia coli, purified and refolded in a soluble form. Crystals of Mala s 1 were obtained in 25% PEG 8K, 0.2 M (NH4)2SO4. Crystals belong to space group P2(1)2(1)2, with unit-cell parameters a = 44.4, b = 163.7, c = 50.6 A, and diffract to 1.35 A resolution.
Vilhelmsson
Monica
M
Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden. monica.vilhelmsson@medks.ki.se
Hallberg
B Martin
BM
Rasool
Omid
O
Zargari
Arezou
A
Scheynius
Annika
A
Achour
Adnane
A
D000485:Allergens / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D000946:Antigens, Fungal / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008290:Malassezia / Q000276:immunology
D000485:Allergens; D000946:Antigens, Fungal; D005656:Fungal Proteins; C510167:Mala s 1 allergen, Malassezia sympodialis