Plant regeneration after long-term callus culture in clones of Asparagus officinalis L.
Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg x l(-1) kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg x l(-1) 2,4-D and 1 mg x l(-1) kinetin. Shoot primordia (SP) induction from > 18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained with clone "265" on MS medium with 5 mg x l(-1) 2iP, 1 mg x l(-1) IAA, 3% sucrose and 0.9% agar. The highest percentage of root induction (100%) was achieved with clone '265' on MS medium with 0.1 mg x l(-1) kinetin, 0.1 mg x l(-1) NAA, 1.32 mg x l(-1) ancymidol, 7% glucose and 0.8% agar. Important medium x genotype interactions were detected, pointing to the need of adjusting this and other in vitro protocols for specific asparagus genotypes.
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Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria and Facultad de Ciencias Agrarias (FCA), Universidad Nacional de Mar del Plata, C. C. 276, (7620) Balcarce, Argentina.
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D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D027761:Asparagus Plant / Q000254:growth & development* / Q000502:physiology*; D001593:Benzyl Compounds; D002999:Clone Cells; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods; D003583:Cytokinins / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology; D011687:Purines; D012038:Regeneration; D013997:Time Factors
D001593:Benzyl Compounds; D003470:Culture Media; D003583:Cytokinins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; C030737:indoleacetic acid; C480551:benzylaminopurine; D007701:Kinetin