title: Antithrombogenic properties of bioconjugate streptokinase-polyglycerol dendrimers.
abstract: Dendrimers are monodisperse, spherical and hyperbranched synthetic macromolecules with a large number of surface groups that have the potential to act as carriers for drug immobilization by covalent binding or charge transfer complexation. In this work, a bioconjugate of streptokinase and a polyglycerol dendrimer (PGLD) generation 5 was used to obtain fibrinolytic surfaces. The PGLD dendrimer was synthesized by the ring opening polymerization of deprotonated glycidol using polyglycerol as core functionality in a step-growth processes denominated divergent synthesis. The PGLD dendritic structure was confirmed by gel permeation chromatography (GPC), nuclear magnetic resonance (1H-NMR, 13C-NMR) and matrix assisted laser desorption/ionization (MALDI-TOF) techniques. The synthesized dendrimer presented low dispersion in molecular weights (Mw/Mn = 1.05) and a degree of branching of 0.82 which characterize the polymer dendritic structure. The blood compatibility of the bioconjugate PGLD-Sk was evaluated by in vitro assays such as platelet adhesion and thrombus formation. Uncoated polystyrene -microtitre plates (ELISA) was used as reference. The epifluorescence microscopy results indicate that PGLD-Sk coating showed an improved antithrombogenic character relative to the uncoated ELISA plates.
authors.affiliation: Departamento de Física e Química, Instituto de Ciências Exatas, Universidade Federal de Itajubá (UNIFEI), Av. BPS. 1303, 37500-903, Itajubá, Minas Gerais, Brasil.
authors.forename: Edson Giuliani Ramos
authors.initials: EG
authors.lastname: Fernandes
authors.forename: Alvaro Antonio Alencar
authors.initials: AA
authors.lastname: de Queiroz
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Abraham
authors.forename: Julio
authors.initials: J
authors.lastname: San Román
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001792:Blood Platelets / Q000378:metabolism; D050091:Dendrimers / Q000737:chemistry; D004339:Drug Compounding; D016503:Drug Delivery Systems; D004797:Enzyme-Linked Immunosorbent Assay; D004800:Enzymes, Immobilized; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D005990:Glycerol / Q000737:chemistry*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D019007:P-Selectin / Q000096:biosynthesis; D010973:Platelet Adhesiveness; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013300:Streptokinase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D050091:Dendrimers; D004800:Enzymes, Immobilized; D005343:Fibrinolytic Agents; D019007:P-Selectin; D011108:Polymers; C043941:polyglycerol; D013300:Streptokinase; D005990:Glycerol