title: Crystallization and preliminary X-ray diffraction analysis of the lectin from Dioclea rostrata Benth seeds.
abstract: Lectins from the Diocleinae subtribe (Leguminosae) are highly similar proteins that promote various biological activities with distinctly differing potencies. The structural basis for this experimental data is not yet fully understood. Dioclea rostrata lectin was purified and crystallized by hanging-drop vapour diffusion at 293 K. The crystal belongs to the orthorhombic space group I222, with unit-cell parameters a = 61.51, b = 88.22, c = 87.76 A. Assuming the presence of one monomer per asymmetric unit, the solvent content was estimated to be about 47.9%. A complete data set was collected at 1.87 A resolution.
authors.affiliation: BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Caixa Postal 6043, CEP 60455-970, Brazil.
authors.forename: Plínio
authors.initials: P
authors.lastname: Delatorre
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: Nascimento
authors.forename: Luciana Magalhães
authors.initials: LM
authors.lastname: Melo
authors.forename: Emmanuel Prata de
authors.initials: EP
authors.lastname: Souza
authors.forename: Bruno Anderson Matias da
authors.initials: BA
authors.lastname: Rocha
authors.forename: Raquel G
authors.initials: RG
authors.lastname: Benevides
authors.forename: Taiana Maia de
authors.initials: TM
authors.lastname: Oliveira
authors.forename: Gustavo Arruda
authors.initials: GA
authors.lastname: Bezerra
authors.forename: Maria Júlia Barbosa
authors.initials: MJ
authors.lastname: Bezerra
authors.forename: Rodrigo Maranguape Silva da
authors.initials: RM
authors.lastname: Cunha
authors.forename: Francisco Assis Bezerra da
authors.initials: FA
authors.lastname: Cunha
authors.forename: Valder Nogueira
authors.initials: VN
authors.lastname: Freire
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D007887:Fabaceae / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry*
chemical_list: D037121:Plant Lectins