High-resolution diffraction from crystals of a membrane-protein complex: bacterial outer membrane protein OmpC complexed with the antibacterial eukaryotic protein lactoferrin.
Crystals of the complex formed between the outer membrane protein OmpC from Escherichia coli and the eukaryotic antibacterial protein lactoferrin from Camelus dromedarius (camel) have been obtained using a detergent environment. Initial data processing suggests that the crystals belong to the hexagonal space group P6, with unit-cell parameters a = b = 116.3, c = 152.4 A, alpha = beta = 90, gamma = 120 degrees. This indicated a Matthews coefficient (VM) of 3.3 A3 Da(-1), corresponding to a possible molecular complex involving four molecules of lactoferrin and two porin trimers in the unit cell (4832 amino acids; 533.8 kDa) with 63% solvent content. A complete set of diffraction data was collected to 3 A resolution at 100 K. Structure determination by molecular replacement is in progress. Structural study of this first surface-exposed membrane-protein complex with an antibacterial protein will provide insights into the mechanism of action of OmpC as well as lactoferrin.
Sundara Baalaji
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N
Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India.
Acharya
K Ravi
KR
Singh
T P
TP
Krishnaswamy
S
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002162:Camelus; D003460:Crystallization; D004058:Diffusion; D004926:Escherichia coli / Q000737:chemistry*; D007781:Lactoferrin / Q000737:chemistry*; D018272:Porins / Q000737:chemistry*; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; C113724:OmpC protein; D018272:Porins; D007781:Lactoferrin