title: Surface properties of dioleoyl-sn-glycerol-3-ethylphosphocholine, a cationic phosphatidylcholine transfection agent, alone and in combination with lipids or DNA.
abstract: Long-chain cationic amphipaths are routinely used for transfecting DNA into cells, although the mechanism of DNA delivery by these agents is poorly understood. Since their interfacial properties are undoubtedly involved at some stage in the process, a comprehensive study of the surface behavior of at least one of these compounds is highly desirable. Hence, the behavior of the cationic transfection agent EDOPC (dioleoyl-sn-glycerol-3-ethylphosphocholine or O-ethyldioleoylphosphatidylcholine), has been characterized at the air-water interface, by itself and in mixtures with other phospholipids. Surface pressure-molecular area isotherms obtained at the argon-buffer interface revealed that EDOPC is considerably (5-10 A(2)) more expanded than the parent phosphatidylcholine (DOPC) and even more expanded than the corresponding phosphatidylglycerol (DOPG), which has a similar charge density (of opposite polarity) as EDOPC. A 1:1 mixture of EDOPC and DOPG is very slightly condensed relative to DOPG and considerably condensed relative to EDOPC. The surface/dipole potential of this mixture is the mean of those of EDOPC and DOPG and is almost the same as that of DOPC. When the composition of EDOPC mixtures was varied, several surface parameters, including surface dipole moment, collapse pressure, and compressibility, exhibited discontinuities at a 1:1 mole ratio. EDOPC is unusually surface-active; the equilibrium surface tension of its dispersion was lower and the rate of fall of the surface tension (dynamic surface activity) of a dispersion with an initially clean surface was more than an order of magnitude greater than that for dispersions of DOPG. A 1:1 mixture of the cationic lipoid and phosphatidylglycerol had lower surface activity than DOPC in water but similar surface activity in 0.1 NaCl. Analysis, in terms of surface concentration, of the formation of EDOPC monolayers at the air interface of vesicle dispersions revealed a simple exponential rise to a maximum, at least for higher concentrations. Addition of a small proportion of DNA to EDOPC increased its dynamic surface activity even though DNA alone has no detectable surface activity at the concentrations used. This enhancement by DNA is presumably due to the disruption of the continuity of the bilayer and creation of defects from which lipoid spreads readily. The surface properties of this cationic compound, both alone and in combination with anionic lipids, provide insight into the previously described nonbilayer phase preferences of cationic-anionic lipid mixtures. In addition, they provide critical data (area condensation of mixed cationic-anionic monolayers) supporting a previously proposed mechanism of fusion of cationic bilayers with anionic bilayers. Such a process, involving anionic cellular membranes, is believed to be required for release of DNA from lipoplexes and is therefore a key stage of transfection.
authors.affiliation: Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500, USA. macd@northwestern.edu
authors.forename: Robert C
authors.initials: RC
authors.lastname: Macdonald
authors.forename: Alex
authors.initials: A
authors.lastname: Gorbonos
authors.forename: Maureen M
authors.initials: MM
authors.lastname: Momsen
authors.forename: Howard L
authors.initials: HL
authors.lastname: Brockman
mesh_terms: D001128:Argon; D004247:DNA / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D013816:Thermodynamics; D014162:Transfection; D014867:Water
chemical_list: D008055:Lipids; D010713:Phosphatidylcholines; D014867:Water; D001128:Argon; D004247:DNA; C017251:1,2-oleoylphosphatidylcholine