title: Rescue and regeneration of injured peripheral nerve axons by intrathecal insulin.
abstract: Insulin peptide, acting through tyrosine kinase receptor pathways, contributes to nerve development or repair. In this work, we examined the direction, impact and repertoire of insulin signaling in vivo during peripheral nerve regeneration in rats. First, we demonstrated that insulin receptor is expressed on lumbar dorsal root ganglia neuronal perikarya using immunohistochemistry. Immunoblots and polymerase chain reactions confirmed the presence of both alpha and beta insulin receptor subunits in dorsal root ganglia. In vivo and in vitro assessment of dorsal root ganglion neurons showed preferential localization of insulin receptor to perikaryal sites. In vivo, intrathecal delivery of fluorescein isothiocyanate-labeled insulin identified localization around dorsal root ganglia neurons. The direction and impact of potential insulin signaling was evaluated by concurrently delivering insulin or carrier over a 2 week period using mini-osmotic pumps, either intrathecally, near nerve, or with both deliveries, following a selective sural nerve crush injury. Only intrathecal insulin increased the number and maturity of regenerating sensory sural nerve axons distal to the crush site. As well, only intrathecal insulin rescued retrograde loss of sural axons after crush. In a separate experiment, insulin also rescued retrograde loss and atrophy of deep peroneal, largely motor, axons post-injury. Intrathecal insulin increased the expression of calcitonin-gene-related peptide in regenerating sprouts, increased the number of visualized regenerating fiber clusters, and reduced downregulation of calcitonin-gene-related peptide in dorsal root ganglia neurons. Insulin delivered intrathecally does not appear to influence expression of insulin-like growth factor-1 at dorsal root ganglion neurons or near peripheral nerve injury, but was associated with upregulation of insulin receptor alpha subunit in dorsal root ganglia. Intrathecal insulin delivery was associated with greater recovery of thermal sensation and longer distances to stimulus response with the pinch test following sural nerve crush. Insulin signaling at neuron perikarya can drive distal sensory axon regrowth, rescue retrograde alterations of axons and alter axon peptide expression. Moreover, such actions are associated with upregulation of its own receptor.
authors.affiliation: Department of Clinical Neurosciences and the Hotchkiss Brain Institute, University of Calgary, Room 168, 3330 Hospital Drive, NW, Calgary, Alberta, Canada T2N 4N1.
authors.forename: C
authors.initials: C
authors.lastname: Toth
authors.forename: V
authors.initials: V
authors.lastname: Brussee
authors.forename: J A
authors.initials: JA
authors.lastname: Martinez
authors.forename: D
authors.initials: D
authors.lastname: McDonald
authors.forename: F A
authors.initials: FA
authors.lastname: Cunningham
authors.forename: D W
authors.initials: DW
authors.lastname: Zochodne
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D001522:Behavior, Animal; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000627:therapeutic use; D007839:Functional Laterality / Q000502:physiology; D005727:Ganglia, Spinal / Q000166:cytology; D005786:Gene Expression Regulation / Q000187:drug effects; D007328:Insulin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D008297:Male; D009409:Nerve Crush / Q000379:methods; D009416:Nerve Regeneration / Q000187:drug effects*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D010147:Pain Measurement / Q000379:methods; D010523:Peripheral Nervous System Diseases / Q000188:drug therapy* / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000502:physiology / Q000528:radiation effects; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism
chemical_list: D007328:Insulin; D012333:RNA, Messenger; C031573:insulin, fluorescein-isothiocyanated-; D011972:Receptor, Insulin; D010751:Phosphopyruvate Hydratase; D016650:Fluorescein-5-isothiocyanate; D015740:Calcitonin Gene-Related Peptide