Magnetic force microscopy analysis of apoptosis of HL-60 cells induced by complex of antisense oligonucleotides and magnetic nanoparticles.
Magnetic force microscopy (MFM) has been employed to observe antisense oligonucleotides (ASOs)-coupled silica-coated magnetic iron oxide nanoparticles (SMNPs) internalized into human leukemia (HL-60) cells. The experiment demonstrated that the ASOs-coupled SMNPs delivery into the cells really occurred. The nanoparticles were internalized into the cells and the apoptotic topography can be directly visualized simultaneously with MFM technology. These present observations offer direct morphology evidence on studying the apoptosis of tumor cells and provide useful information for better design of new diagnostic and therapeutic tools in tumor treatment.
Shen
He-bai
HB
Life and Environment Science College, Shanghai Normal University, Shanghai 200234, PR China. Shenhb@shnu.edu.cn
Long
De-hong
DH
Zhu
Long-zhang
LZ
Li
Xing-Yu
XY
Dong
Ya-ming
YM
Jia
Neng-qin
NQ
Zhou
Hai-qing
HQ
Xin
Xi
X
Sun
Yang
Y
D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D005290:Ferric Compounds / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000473:pathology; D008280:Magnetics; D020527:Microscopy, Scanning Probe / Q000295:instrumentation* / Q000379:methods; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry
D005290:Ferric Compounds; D016376:Oligonucleotides, Antisense; C000499:ferric oxide; D012822:Silicon Dioxide