[Study on protective effect of tetrandrine on lipopolysaccharide induced pancreatic acinar cell damage and its mechanism].
OBJECTIVE
To evaluate the protective effect of tetrandrine (Tet) on lipopolysaccharide (LPS)-induced pancreatic acinar cell damage and to explore its mechanism.


METHODS
SD male rat's pancreatic acinar cells were isolated by collagenase digestion and they were pre-treated with Tet (50 micromol/L and 100 micromol/L), then exposed to LPS (10 mg/L) or conventional culture medium respectively. At 0, 1, 4, 10 hours after treatment with the agents, cell viability was determined by methyl thiazolyl tetrazolium (MTT), and the supernatant supernate of cells was collected for determination of the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) and phospholipase A(2) (PLA(2)). Some cells were loaded with Fluo-3/AM, and the dynamic change in intracellular Ca(2+) ([Ca(2+)]i) in single pancreatic acinar cell was determined by laser scanning confocal microscopy.


RESULTS
Tet attenuated LPS-induced cell damage (P<0.05 and P<0.01) and inhibited the elevation of cytosolic free calcium of rat pancreatic acinar cells. In the supernatant, Tet pretreatment decreased the content of MDA and the activity of PLA(2) and increased the activity of SOD (all P<0.05).


CONCLUSIONS
Tet attenuates LPS-induced cell damage by blocking [Ca(2+)]i overload, inhibiting superoxide response, decreasing activity of pancreatic enzyme, thus it shows a protective effect on pancreatic acinar cells.
Zhang
Hong
H
Department of Pathophysiology, Shanxi College of Traditional Chinese Medicine, Xianyang 712046, Shanxi, China. zhangh1227@sohu.com
Li
Yong-yu
YY
Wu
Xian-zhong
XZ
D000818:Animals; D044182:Benzylisoquinolines / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D054467:Phospholipases A2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
D044182:Benzylisoquinolines; D008070:Lipopolysaccharides; C009438:tetrandrine; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D054467:Phospholipases A2; D002118:Calcium