title: Inflammatory lesion and parasite load are inversely associated in Leishmania amazonensis infected mice genetically selected according to oral tolerance susceptibility.
abstract: Two strains of mice selected according to extreme phenotypes of susceptibility and resistance to oral tolerance (TS and TR mice, respectively) were infected with 1 x 10(7) Leishmania amazonensis promastigotes and studied comparatively. TS mice developed a minor pathology while permitting parasite growth with the presence of increased IL-4, IL-10 and IFN-gamma, and lower NO and IL-2 levels and delayed-type hypersensitivity (DTH). In contrast, in TR mice, footpad swelling was increased but parasite growth was reduced. They produced lower IL-4, IL-10 and IFN-gamma but increased NO, IL-2 levels, DTH, activated spleen macrophages and periarteriolar lymphoid sheaths. The results suggest that the tolerogenic TS mouse profile, with higher IL-10 production, impaired lesion development but also avoided macrophage leishmanicidal activity, maintaining in this manner a silent parasite load. On the other hand, the TR mouse profile contributed to lesion progression with controlled parasite load. To directly address the influence of oral tolerance on infection, mice were gavaged with OVA, and 7 days afterwards were infected and challenged to bystander suppression with OVA in the same footpad. In TR mice gavaged with 25 mg OVA the inflammatory lesion was largely enhanced, while with 5 mg OVA the lesion was diminished. In TS mice the footpad swelling was always lower. However, the bystander effect did not modify the establishment of infection; and similarly to the control non-bystander mice, parasite clearance was maintained in TR and prevented in TS mice. Therefore, a better comprehension of immunoregulation of innate and adaptive immunity in the early stages of infection is necessary for the development of protocols preventing inflammation and contributing to the elimination of parasites.
authors.affiliation: Laboratório de Imunobiologia, Departamento Biologia Celular e Genética, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Rua S. Francisco Xavier, 524, PHLC, Maracanã, CEP 20559-900 Rio de Janeiro, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Tavares
authors.forename: Ricardo
authors.initials: R
authors.lastname: da Conceição Ribeiro
authors.forename: Antonio
authors.initials: A
authors.lastname: Carlos da Silva
mesh_terms: D000284:Administration, Oral; D000818:Animals; D021461:Animals, Outbred Strains; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D020022:Genetic Predisposition to Disease; D007108:Immune Tolerance / Q000235:genetics*; D007249:Inflammation / Q000473:pathology; D007891:Leishmania / Q000276:immunology / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000235:genetics* / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology; D010270:Parasite Egg Count; D010587:Phagocytosis; D012641:Selection, Genetic; D012867:Skin / Q000469:parasitology / Q000473:pathology; D013154:Spleen / Q000276:immunology
chemical_list: D016207:Cytokines; D009569:Nitric Oxide; D010047:Ovalbumin