title: Expression, renaturation and functional analysis of an excitatory insect-specific toxin from scorpion Buthus martensii Karsch.
abstract: The cDNA of BmK IT-AP, an excitatory insect toxin from the scorpion Buthus martensi Karsch that has an analgesic effect on mammalian cells, was expressed in E. coli in the form of an inclusion body. Following denaturation and reduction, the recombinant protein was renatured and purified by liquid chromatography. The authenticity of the recombinant product was confirmed by bioassay and its electrophysiological effect on insect sodium channel.
authors.affiliation: Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, People's Republic of China.
authors.forename: Chong
authors.initials: C
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Frank
authors.initials: F
authors.lastname: Bossmans
authors.forename: Rong-Huan
authors.initials: RH
authors.lastname: Zhu
authors.forename: Jan
authors.initials: J
authors.lastname: Tytgat
authors.forename: Da-Cheng
authors.initials: DC
authors.lastname: Wang
mesh_terms: D000818:Animals; D004594:Electrophysiology; D015870:Gene Expression; D007814:Larva / Q000187:drug effects; D009036:Moths / Q000187:drug effects; D009865:Oocytes; D018408:Patch-Clamp Techniques; D020673:Protein Renaturation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity; D012604:Scorpion Venoms / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity*; D012605:Scorpions / Q000737:chemistry* / Q000235:genetics; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D011994:Recombinant Proteins; D012604:Scorpion Venoms; D015222:Sodium Channels