Characterization of 23 CC chemokine genes and analysis of their expression in channel catfish (Ictalurus punctatus).
Chemokines are a large family of chemotactic cytokines playing crucial roles in the innate immune response. CC chemokines constitute the largest subfamily of chemokines, with 28 CC chemokines identified from mammalian species. However, the status of CC chemokines in teleosts is yet to be determined. We previously identified 26 catfish CC chemokine cDNAs from catfish. In this study, we isolated and sequenced 23 channel catfish CC chemokine genes amounting to a total of over 56 kb of genomic sequences. Genomic organization of the 23 CC chemokine genes was determined by comparing the generated genomic sequences with the previously identified cDNA sequences. Microsatellites were identified from 16 catfish CC chemokine genes allowing them to be utilized for genome mapping. Structural analysis indicated conservation of genomic organization of CC chemokine genes, which may facilitate the establishment of orthologies. Expression of all known catfish CC chemokine transcripts was assessed in nine important tissues. Of the 26 catfish CC chemokine genes, 14 were universally expressed, six were widely expressed in many tissues, while six were highly tissue-specific.
Bao
Baolong
B
The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849, USA.
Peatman
Eric
E
Peng
Xu
X
Baoprasertkul
Puttharat
P
Wang
Guiling
G
Liu
Zhanjiang
Z
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019742:Chemokines, CC / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D017931:DNA Primers; D020869:Gene Expression Profiling; D015723:Gene Library; D007059:Ictaluridae / Q000235:genetics* / Q000276:immunology*; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA
D019742:Chemokines, CC; D017931:DNA Primers; D012333:RNA, Messenger