Interleukin-1beta gene polymorphism associated with hepatocellular carcinoma in hepatitis B virus infection.
OBJECTIVE
To examine the effect of interleukin-1-beta (IL-1beta) promoter region C-511T and IL-1 receptor antagonist (IL-1RN) polymorphism among the patients with chronic hepatitis B virus (HBV) infection (HCC and non-HCC).


METHODS
Genomic DNA from 136 Thai patients with chronic HBV infection (HCC=46 and non-HCC=90) and 152 healthy individuals was genotyped for IL-1beta gene polymorphism (-511) using polymerase chain reaction with sequence specific primers (PCR-SSP). The variable number of tandem repeats (VNTR) of IL-1RN gene was assessed by a PCR-based assay. The association between these genes and status of the disease was evaluated by chi2 test.


RESULTS
IL-1B-511 genotype C/C was found to be significantly different in patients with HCC when compared with healthy individuals (P=0.036, OR=2.29, 95%CI=1.05-4.97) and patients without HCC (P=0.036, OR=2.52, 95%CI=1.05-6.04). Analysis of allele frequencies of IL-1B-511 showed that IL-1B-511 C allele was also significantly increased in patients with HCC, compared to that in healthy control (P=0.033, OR=1.72, 95%CI=1.04-2.84). However, no significant association in IL-1RN gene was found between the two groups.


CONCLUSIONS
IL-1B-511C allele, which may be associated with high IL-1B production in the liver, is a genetic marker for the development of HCC in chronic hepatitis B patients in Thai population.
Hirankarn
Nattiya
N
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Rama IV Road, Bangkok 10330, Thailand. fmednpt@md.chula.ac.th
Kimkong
Ingorn
I
Kummee
Pittaya
P
Tangkijvanich
Pisit
P
Poovorawan
Yong
Y
D000483:Alleles; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000150:complications / Q000235:genetics / Q000276:immunology*; D016022:Case-Control Studies; D005787:Gene Frequency; D019694:Hepatitis B, Chronic / Q000150:complications / Q000235:genetics / Q000276:immunology*; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics*; D008113:Liver Neoplasms / Q000150:complications / Q000235:genetics / Q000276:immunology*; D018598:Minisatellite Repeats; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012795:Sialoglycoproteins / Q000235:genetics; D013785:Thailand
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D012795:Sialoglycoproteins