title: Comparative analyses of canine distemper viral isolates from clinical cases of canine distemper in vaccinated dogs.
abstract: Sequence and phylogenetic analyses of three isolates of canine distemper virus (CDV) isolated from three dogs with a vaccination history were compared with the same analyses of vaccine virus isolated from a vaccine used for dogs. The three dogs showed clinical signs of a recent major type of CD in Japan, including oculonasal discharge and diarrhea, and pathological findings including non-suppurative encephalitis, pneumonia, mild gastroenteritis and lymphoid depletion. Inclusion bodies were in the stomach without inflammation and encephalitis was without clinical signs. One of the highest titers of CDV in different organs of the three dogs was commonly systemic lymphatic organs, including the spleen, lymph nodes and tonsils. New isolates of CDV joined to the clades of the Asia 1 group that is far from the vaccine group. These results surely indicate that wild strains of CDV from dogs with a vaccination history were not reversed vaccine virus, and that the dogs showed characteristics of recent CD in Japan.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: N T
authors.initials: NT
authors.lastname: Lan
authors.forename: R
authors.initials: R
authors.lastname: Yamaguchi
authors.forename: A
authors.initials: A
authors.lastname: Inomata
authors.forename: Y
authors.initials: Y
authors.lastname: Furuya
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: S
authors.initials: S
authors.lastname: Sugano
authors.forename: S
authors.initials: S
authors.lastname: Tateyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D004216:Distemper / Q000175:diagnosis* / Q000473:pathology / Q000517:prevention & control / Q000821:virology; D004217:Distemper Virus, Canine / Q000145:classification* / Q000302:isolation & purification*; D004285:Dogs; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000235:genetics; D007150:Immunohistochemistry / Q000662:veterinary; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014709:Vero Cells; D014765:Viral Vaccines
chemical_list: D006389:Hemagglutinins, Viral; D010750:Phosphoproteins; D014765:Viral Vaccines