Determination of four nitroimidazoles in poultry and swine muscle and eggs by liquid chromatography/tandem mass spectrometry.
A rapid method is presented for the determination of 4 nitroimidazoles in poultry and swine muscle and eggs by liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS). Samples were extracted with acetonitrile, then evaporated the organic phase. After filtration, the extract was directly injected into the LC/ESI-MS/MS system. The LC separation was made on a C8 column by applying a gradient composed of water and acetonitrile. Overall average recoveries ranged from 50-86% for egg, 66-115% for poultry muscle, and 79-111% for swine muscle. The decision limits ranged from 0.05-0.25 microg/kg for egg, 0.07-0.27 microg/kg for poultry muscle, and 0.07-0.26 microg/kg for swine muscle; and detection capabilities ranged from 0.51-0.68 microg/kg for egg, 0.41-0.75 microg/kg for poultry muscle, and 0.53-0.78 microg/kg for swine muscle.
Xia
Xi
X
China Agricultural University, College of Veterinary Medicine, Department of Pharmacology and Toxicology, Beijing 100094, People's Republic of China.
Li
Xiaowei
X
Shen
Jianzhong
J
Zhanc
Suxia
S
Ding
Shuangyang
S
Jiang
Haiyang
H
D000097:Acetonitriles / Q000032:analysis; D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D004531:Eggs; D005504:Food Analysis / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009132:Muscles / Q000378:metabolism; D009593:Nitroimidazoles / Q000032:analysis*; D011200:Poultry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine; D013997:Time Factors; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D009593:Nitroimidazoles; D014867:Water; C032159:acetonitrile