title: [Determination of opiates in biological human samples by liquid chromatography-tandem mass spectrometry].
abstract: OBJECTIVE
  Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of opiates in biological samples according to the emerging problem in drugs abuse.
  
  
  METHODS
  Opiates such as heroin, 6-acetylmorphine, morphine, codeine, acetylcodeine, hydrocodone and hydromorphone were isolated from human blood, urine, oral fluid and hair using a simple extraction and consequently analyzed using LC-MS/MS. The method was evaluated by real cases.
  
  
  RESULTS
  The mobile phase give the optimum separation for opiates. The detection limit of morphine in urine with dilution and liquid-liquid extraction and in hair is 10ng/mL, 0.01 ng/mL and 0.01 ng/mg, respectively.
  
  
  CONCLUSIONS
  The method is simple and rapid, offering superior sensitivity and selectivity for opiates. The target compounds comprising hydrocodone and hydromorphone enlarge the applied area.
authors.affiliation: Institute of Forensic Sciences, Ministry of Justice, P.R.China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xiang
authors.forename: Min
authors.initials: M
authors.lastname: Shen
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Shen
authors.forename: Dong
authors.initials: D
authors.lastname: Ma
authors.forename: Jun
authors.initials: J
authors.lastname: Bu
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Xian-yi
authors.initials: XY
authors.lastname: Zhuo
mesh_terms: D002853:Chromatography, Liquid; D003061:Codeine / Q000032:analysis; D005554:Forensic Medicine / Q000379:methods; D006197:Hair / Q000737:chemistry*; D006801:Humans; D006853:Hydrocodone / Q000032:analysis; D004091:Hydromorphone / Q000032:analysis; D009020:Morphine / Q000032:analysis; D009294:Narcotics / Q000032:analysis*; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D009294:Narcotics; D006853:Hydrocodone; D009020:Morphine; D004091:Hydromorphone; D003061:Codeine