Electron paramagnetic resonance decay constant and oxidative stresses in liver microsomes of the selenium-deficient rat.
The free radical-reducing activity and the membrane fluidity of liver microsomes from selenium-deficient (SeD) rats were examined by means of electron paramagnetic resonance (EPR) spin label method using nitroxyl-labeled stearic acids. Our findings show that the membrane fluidity and lipid peroxidation levels in SeD rat liver microsome were relatively unchanged compared with normal rat. In contrast, SeD caused the induction of liver microsomal cytochrome P-450 activity. The nitroxyl spin probes are substrates for reduction-relating cytochrome P-450. Previous in vivo studies suggested that the total liver free radical reduction activity in SeD rat was decreased. In contrast, SeD caused the induction of liver microsomal cytochrome P-450 activity, and the reduction rate of nitroxyl radical existing at shallow depth in membrane was increased. Selenium-deficient rats experienced an increase in hydrogen peroxide (H2O2) due to a pronounced loss of glutathione peroxidase (GSH-Px) activity. This masked the overall reduction rate of the nitroxyl spin probe by reoxidation of the hydroxylamine form. Although the SeD condition caused induction of liver cytochrome P-450 and chronic increased H2O2, this did not result in oxidative liver damage. An increased level of glutathione in SeD liver was also evident, likely due to the absence of GSH-Px activity. Using the EPR spin label method, we have shown that SeD causes complicated redox changes in the liver, notably, alterations in the levels of cytochrome P-450 and GSH-Px systems.
Matsumoto
Ken-ichiro
K
Department of Physical Chemistry, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan.
Suzuki
Ayako
A
Washimi
Haruka
H
Hisamatsu
Atsushi
A
Okajo
Aya
A
Ui
Iori
I
Endo
Kazutoyo
K
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004032:Diet; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D008560:Membrane Fluidity; D008862:Microsomes, Liver / Q000378:metabolism* / Q000648:ultrastructure; D009589:Nitrogen Oxides; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage / Q000172:deficiency*; D013113:Spin Labels; D013229:Stearic Acids; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D009589:Nitrogen Oxides; D013113:Spin Labels; D013229:Stearic Acids; D017392:Thiobarbituric Acid Reactive Substances; C031183:stearic acid; D003577:Cytochrome P-450 Enzyme System; D005979:Glutathione Peroxidase; D005978:Glutathione; C039900:nitroxyl; D012643:Selenium