[Cloning of the conserved fragment of urease gene of Staphylococcus warneri].
OBJECTIVE
To obtain the clone of conserved fragment of urease gene of Staphylococcus warneri.


METHODS
Urease activity was measured by phenol red assay. An internal fragment of the urease gene of Staphylococcus warneri was amplified by PCR with primers derived from conserved amino acid sequences of the large subunit of urease. The PCR product was then cloned into T-vector and sequenced.


RESULTS
Urease activity of Staphylococcus warneri was positive. The internal fragment of urease gene of Staphylococcus warneri was highly homologous to known urease gene cluster of other bacteria.


CONCLUSIONS
In this study, urease activity of Staphylococcus warneri was proved and the internal fragment of urease gene encoding conserved amino acid sequences was cloned.
Wang
Yan
Y
Department of Preventive and Pediatric Dentistry, School of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai 200011, China.
Feng
Xi-ping
XP
Li
Ming-yu
MY
Huang
Zheng-wei
ZW
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D013210:Staphylococcus / Q000235:genetics*; D014510:Urease / Q000235:genetics*
D014510:Urease