title: Crystallization and halide phasing of the C-terminal domain of human KIN17.
abstract: Here, the crystallization and initial phasing of the C-terminal domain of human KIN17, a 45 kDa protein mainly expressed in response to ionizing radiation and overexpressed in certain tumour cell lines, are reported. Crystals diffracting to 1.4 A resolution were obtained from 10% ethylene glycol, 27% PEG 6000, 500 mM LiCl and 100 mM sodium acetate pH 6.3 in space group P2(1)2(1)2(1), with unit-cell parameters a = 45.75, b = 46.31, c = 60.80 A and one molecule in the asymmetric unit. Since this domain has a basic pI, heavy-atom derivatives were obtained by soaking the crystals with negatively charged ions such as tungstate and iodine. The replacement of LiCl by KI in the cryosolution allowed the determination of phases from iodide ions to give an interpretable electron-density map.
authors.affiliation: Département d'Ingénierie et d'Etude des Protéines, Commissariat a l'Energie Atomique, 91191 Gif-sur-Yvette, France. albane.lemaire@cea.fr
authors.forename: Albane
authors.initials: A
authors.lastname: le Maire
authors.forename: Marc
authors.initials: M
authors.lastname: Schiltz
authors.forename: Sandrine
authors.initials: S
authors.lastname: Braud
authors.forename: Muriel
authors.initials: M
authors.lastname: Gondry
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Charbonnier
authors.forename: Sophie
authors.initials: S
authors.lastname: Zinn-Justin
authors.forename: Enrico
authors.initials: E
authors.lastname: Stura
mesh_terms: D002712:Chlorides / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006801:Humans; D007454:Iodides / Q000737:chemistry; D009687:Nuclear Proteins / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D017434:Protein Structure, Tertiary / Q000187:drug effects; D016601:RNA-Binding Proteins; D017973:Tungsten Compounds / Q000737:chemistry
chemical_list: D002712:Chlorides; D004268:DNA-Binding Proteins; D007454:Iodides; C489487:KIN protein, human; D009687:Nuclear Proteins; D010710:Phosphates; D016601:RNA-Binding Proteins; D017973:Tungsten Compounds; C045951:tungstate