[Isolation of a novel peptide that binds to BLyS from a 12-mer phage display peptide library].
A 12-mer phage display peptide library was screened for specific binders against B lymphocyte stimulator (BLyS). After 3 rounds of panning, positive phage clones were enriched. ELISAs were used to identify positive phages, and the DNA encoding the positive peptide (RHKIQLRQNIIT) was cloned and expressed as a GST fusion protein in E. coli. After purification, the identity of the fusion protein was confirmed through its specific binding to BLyS by ELISA. We have obtained a peptide that can bind to BLyS and probably act as an antagonistic peptide against the natural BLyS receptor.
Ding
Yan-Li
YL
School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. dingylding@yahoo.com.cn
Han
Wei
W
Shen
Qiong
Q
Liu
Hui
H
Yang
Sheng-Li
SL
Gong
Yi
Y
D053264:B-Cell Activating Factor / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism*; D011485:Protein Binding
D053264:B-Cell Activating Factor; D019151:Peptide Library; D010455:Peptides