Stokes polarimetry imaging of rat tail tissue in a turbid medium: degree of linear polarization image maps using incident linearly polarized light.
Illumination with incident linearly polarized light on tissue and polarization state measurements of the remitted light provide a means by which various tissue structures can be differentiated. A rat tail is embedded within a turbid gelatin such that there is a variable depth of medium above it. By varying the incident polarization angle (IPA) of the illuminating linearly polarized light, the geometry, and the orientation angle of the tissue, a series of 2-D degree of linear polarization image maps are created using our Stokes polarimetry imaging technique. The image maps show locations of the polarization-sensitive structures in the rat tail, including soft tissue, intervertebral disks, and tendons. The observed morphologies in the image maps indicate locations where the depolarization of light differs according to the tissue type and underlying layers. The data indicate the importance of varying the IPA, and that tissue dichroism and birefringence affect the degree of linear polarization image maps. Diagnostic information regarding subsurface tissue structures is obtained.
Wu
Paul J
PJ
Northwestern University, Biomedical Engineering Department, Evanston, Illinois 60208, USA.
Walsh
Joseph T
JT
D000818:Animals; D066298:In Vitro Techniques; D008859:Microscopy, Polarization / Q000379:methods*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012031:Refractometry / Q000379:methods*; D013623:Tail / Q000166:cytology* / Q000502:physiology*