Purification, crystallization and preliminary crystallographic analysis of the Hermes transposase.
DNA transposition is the movement of a defined segment of DNA from one location to another. Although the enzymes that catalyze transposition in bacterial systems have been well characterized, much less is known about the families of transposase enzymes that function in higher organisms. Active transposons have been identified in many insect species, providing tools for gene identification and offering the possibility of altering the genotypes of natural insect populations. One of these active transposons is Hermes, a 2749-base-pair element from Musca domestica that encodes its own transposase. An N-terminally deleted version of the Hermes transposase (residues 79-612) has been overexpressed and purified, and crystals that diffract to 2.1 A resolution have been obtained at 277 K by the hanging-drop method.
Perez
Zhanita N
ZN
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD, USA.
Musingarimi
Primrose
P
Craig
Nancy L
NL
Dyda
Fred
F
Hickman
Alison Burgess
AB
D000818:Animals; D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods; D006793:Houseflies / Q000201:enzymology*; D017384:Sequence Deletion; D019895:Transposases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014835:Volatilization; D014961:X-Ray Diffraction
D019895:Transposases