[Expression of caspase-8 after brain injury from fluid percussion in rats].
OBJECTIVE
To provide the evidence of the relationship between brain injury and the time of injury.


METHODS
Rats were contused on brain by fluid percussion, then were killed after injury for 15 min, 30 min, 1,3,6,12 h, and 1,4,7,14 d respectively. The expression of caspase-8 were detected by immunohistochemical technology on rat brain section and the results were assessed by image analysis system in the cerebral cortex, thalamus, and hippocampus.


RESULTS
The expression of caspase-8 in cortex and hippocampus could be detected in 30 min after injury, increased significantly in 3h, reached apex in 1d after injury, remained 4d before decreased. In addition, the expression of caspase-8 can be detected in 1h after injury and reached apex in 1d after injury, and remained 4d then reduced.


CONCLUSIONS
It seems that the expression of caspase-8 should be a useful target for diagnosis of early brain injury.
Yang
Jing
J
West China College of Preclinical Medicine and Forensic Sciences of Sichuan University, Chendu 610041, China.
Wang
Ye
Y
Chen
Xiao-gang
XG
Peng
Qi-yi
QY
Li
Lei-bo
LB
Liu
Min
M
D000818:Animals; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D053181:Caspase 8 / Q000032:analysis*; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D016489:Head Injuries, Closed / Q000378:metabolism* / Q000473:pathology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling; D013788:Thalamus / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
C505528:Casp8 protein, rat; D053181:Caspase 8