Multiresidue determination of eleven quinolones in milk by liquid chromatography with fluorescence detection.
A liquid chromatographic method with fluorescence detection was developed for simultaneous determination of norfloxacin, ofloxacin, ciprofloxacin, pefloxacin, lomefloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid, and flumequine in milk The samples were extracted with 10% trichloroacetic acid/acetonitrile (9 + 1, v/v) and cleaned by Strata-X reversed-phase solid-phase extraction cartridges. The 11 quinolones were separated on a reversed-phase C18 column (Hypersil BDS-C18) with mobile phase gradient elution and detected with fluorescence by means of a wavelength program. The recoveries for milk fortified with the 11 quinolones at 3 levels were 69-88% with acceptable relative standard deviations of <9% (intraday) and <14% (interday). The limits of detection were 23 microg/L for enrofloxacin, and 1-9 microg/L for the other 10 quinolones.
Yang
Guixiang
G
South China Agricultural University, College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, Guangzhou, 510642, Guangdong, People's Republic of China.
Lin
Baoyin
B
Zeng
Zhenling
Z
Chen
Zhangliu
Z
Huang
Xianhui
X
D000818:Animals; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002845:Chromatography; D002853:Chromatography, Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000031:analogs & derivatives / Q000032:analysis; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000032:analysis; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D009643:Norfloxacin / Q000032:analysis; D015242:Ofloxacin / Q000032:analysis; D010093:Oxolinic Acid / Q000032:analysis; D015366:Pefloxacin / Q000032:analysis; D015363:Quinolones / Q000032:analysis* / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
D024841:Fluoroquinolones; D015363:Quinolones; D012997:Solvents; C068581:danofloxacin; D015366:Pefloxacin; D000077422:Enrofloxacin; D002939:Ciprofloxacin; C047225:difloxacin; D015242:Ofloxacin; D010093:Oxolinic Acid; C053091:lomefloxacin; D009643:Norfloxacin; C047224:sarafloxacin; C012976:flumequine