title: Retinal nerve fiber layer assessment using optical coherence tomography with active optic nerve head tracking.
abstract: OBJECTIVE
  To develop an eye-motion-tracking optical coherence tomographic (OCT) method and assess its effect on image registration and nerve fiber layer (NFL) thickness measurement reproducibility.
  
  
  METHODS
  A system capable of tracking common fundus features based on reflectance changes was integrated into a commercial OCT unit (OCT II; Carl Zeiss Meditec, Inc., Dublin, CA) and tested on healthy subjects and patients with glaucoma. Twenty successive peripapillary NFL scans were obtained with tracking and 20 without tracking, for 40 images in each session for each eye. Subjects participated in one session on three different days. Composite OCT scans and composite fundus images were generated for assessment of eye tracking. NFL thickness measurement reproducibility was also assessed.
  
  
  RESULTS
  Seven healthy and nine glaucomatous eyes of 16 subjects were recruited. A qualitative assessment of composite OCT scans and composite fundus images showed little motion artifact or blurring along edges and blood vessels during tracking; however, those structures were less clearly defined when tracking was disengaged. There was no significant reproducibility difference with and without tracking in both intra- and intersession NFL measurement SD calculations in any location. The mean retinal pixel SD was significantly smaller with tracking than without (490.9 +/- 19.3 microm vs. 506.4 +/- 31.8 microm, P = 0.005, paired t-test).
  
  
  CONCLUSIONS
  A retinal-tracking system was successfully developed and integrated into a commercial OCT unit. Tracking OCT improved the consistency of scan registration, but did not influence NFL thickness measurement reproducibility in this small sample study.
authors.affiliation: UPMC Eye Center, Eye and Ear Institute, Ophthalmology and Visual Science Research Center, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA. ishikawah@upmc.edu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Gabriele
authors.forename: Gadi
authors.initials: G
authors.lastname: Wollstein
authors.forename: R Daniel
authors.initials: RD
authors.lastname: Ferguson
authors.forename: Daniel X
authors.initials: DX
authors.lastname: Hammer
authors.forename: L Adelina
authors.initials: LA
authors.lastname: Paunescu
authors.forename: Siobahn A
authors.initials: SA
authors.lastname: Beaton
authors.forename: Joel S
authors.initials: JS
authors.lastname: Schuman
mesh_terms: D000328:Adult; D000368:Aged; D003941:Diagnostic Techniques, Ophthalmological; D005133:Eye Movements; D005901:Glaucoma / Q000175:diagnosis*; D006801:Humans; D009412:Nerve Fibers / Q000473:pathology*; D009898:Optic Disk / Q000503:physiopathology*; D015203:Reproducibility of Results; D012165:Retinal Ganglion Cells / Q000473:pathology*; D041623:Tomography, Optical Coherence / Q000379:methods*