title: Preoperative radiotherapy contributes to induction of proliferative activity of CD8+ tumor-infiltrating T-cells in oral squamous cell carcinoma.
abstract: In order to evaluate host immune response to cancer, many methods have been applied. However, in the field of oral squamous cell carcinoma, evaluation of host immune response on the basis of proliferative activity of tumor-infiltrating T-cells (TIL) has not been reported. Therefore, we applied double immunohistochemical staining of proliferation markers Ki-67 and CD8 to surgically resected and paraffin-embedded tissue sections for 35 cases of oral squamous cell carcinoma. With this method, there was a significant correlation between the percentage of Ki-67+CD8+TIL and the intra-tumor epithelium infiltration rate of CD8+TIL (P=0.0237). In the process of analysis, we found that the proliferative activity of CD8+TIL tended to correlate (P=0.0859) with clinical N factor (lymph node metastasis), which was previously reported to suppress host immune response. We therefore assumed there was another factor inducing host immune response. The proliferative activity of CD8+TIL was well correlated with preoperative radiotherapy (P=0.0200) while there was no significant correlation between the proliferative activity of CD8+TIL and other clinical factors; age, tumor size, clinical stage, pathological N factor (P=0.5410, 0.7769, 0.1041, and 0.1072, respectively). Our present results strongly imply that preoperative radiotherapy is a very important factor in oral squamous cell carcinoma inducing host immune response regardless of the clinical factors present.
authors.affiliation: Departments of Oral and Maxillofacial Sciences, Immunopathology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan.
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Suwa
authors.forename: Masanao
authors.initials: M
authors.lastname: Saio
authors.forename: Naoki
authors.initials: N
authors.lastname: Umemura
authors.forename: Tomomi
authors.initials: T
authors.lastname: Yamashita
authors.forename: Makoto
authors.initials: M
authors.lastname: Toida
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shibata
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takami
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016827:CD8 Antigens / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology / Q000532:radiotherapy*; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects*; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D016246:Lymphocytes, Tumor-Infiltrating / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000532:radiotherapy*; D011300:Preoperative Care; D012044:Regression Analysis
chemical_list: D016827:CD8 Antigens; D019394:Ki-67 Antigen