title: Lactulose and mannitol intestinal permeability detected by capillary electrophoresis.
abstract: Aim of this study was to set up a method by capillary electrophoresis to detect lactulose and mannitol in urine after an oral load, and to estimate the intestinal permeability in controls and in type I diabetes patients. The underivatized carbohydrates were monitored by indirect UV detection using sorbate, cetyltrimethylammonium bromide and LiOH as background electrolyte. Urines were purified by solid phase extraction, shaken with cation exchange resin, filtered and analysed. Carbohydrates migrated in <10 min in relation to their pK(a) and M(r). Controls (n = 33) and patients (n = 23) had an excretion ratio lactulose/mannitol 0.025 (0.018-0.051) and 0.067 (0.050-0.127), respectively (p < 0.01, median, interquartile range).
authors.affiliation: Department of Medicine, Surgery and Dental Science, University of Milan, H San Paolo, via Di Rudini' 8, 20142 Milan, Italy. rita.paroni@unimi.it
authors.forename: Rita
authors.initials: R
authors.lastname: Paroni
authors.forename: Isabella
authors.initials: I
authors.lastname: Fermo
authors.forename: Laura
authors.initials: L
authors.lastname: Molteni
authors.forename: Laura
authors.initials: L
authors.lastname: Folini
authors.forename: Matteo Rocco
authors.initials: MR
authors.lastname: Pastore
authors.forename: Andrea
authors.initials: A
authors.lastname: Mosca
authors.forename: Emanuele
authors.initials: E
authors.lastname: Bosi
mesh_terms: D000293:Adolescent; D000328:Adult; D002138:Calibration; D016022:Case-Control Studies; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007408:Intestinal Absorption; D007792:Lactulose / Q000493:pharmacokinetics* / Q000652:urine; D008353:Mannitol / Q000493:pharmacokinetics* / Q000652:urine
chemical_list: D008353:Mannitol; D007792:Lactulose