title: Consecutive use of two multiplex PCR-based assays for simultaneous identification and determination of capsular status of nine common Neisseria meningitidis serogroups associated with invasive disease.
abstract: We developed two Neisseria meningitidis multiplex PCR assays to be used consecutively that allow determination of the serogroup and capsular status of serogroup A, B, C, 29E, W135, X, and Y cnl-3/cnl-1-like-containing N. meningitidis isolates by direct analysis of the amplicon size. These assays offer a rapid and simple method of serogrouping N. meningitidis.
authors.affiliation: Epidemiology and Molecular Biology Unit, The Children's University Hospital, Temple Street, Dublin 1, Ireland.
authors.forename: Désirée E
authors.initials: DE
authors.lastname: Bennett
authors.forename: Mary T
authors.initials: MT
authors.lastname: Cafferkey
mesh_terms: D001483:Base Sequence; D002353:Carrier State / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008589:Meningococcal Infections / Q000382:microbiology; D009345:Neisseria meningitidis / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D038381:Neisseria meningitidis, Serogroup A / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D038541:Neisseria meningitidis, Serogroup B / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D038542:Neisseria meningitidis, Serogroup C / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D038582:Neisseria meningitidis, Serogroup W-135 / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D038581:Neisseria meningitidis, Serogroup Y / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods* / Q000706:statistics & numerical data; D014774:Virulence / Q000235:genetics
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial