title: Magnetic force microscopy analysis of apoptosis of HL-60 cells induced by complex of antisense oligonucleotides and magnetic nanoparticles.
abstract: Magnetic force microscopy (MFM) has been employed to observe antisense oligonucleotides (ASOs)-coupled silica-coated magnetic iron oxide nanoparticles (SMNPs) internalized into human leukemia (HL-60) cells. The experiment demonstrated that the ASOs-coupled SMNPs delivery into the cells really occurred. The nanoparticles were internalized into the cells and the apoptotic topography can be directly visualized simultaneously with MFM technology. These present observations offer direct morphology evidence on studying the apoptosis of tumor cells and provide useful information for better design of new diagnostic and therapeutic tools in tumor treatment.
authors.affiliation: Life and Environment Science College, Shanghai Normal University, Shanghai 200234, PR China. Shenhb@shnu.edu.cn
authors.forename: He-bai
authors.initials: HB
authors.lastname: Shen
authors.forename: De-hong
authors.initials: DH
authors.lastname: Long
authors.forename: Long-zhang
authors.initials: LZ
authors.lastname: Zhu
authors.forename: Xing-Yu
authors.initials: XY
authors.lastname: Li
authors.forename: Ya-ming
authors.initials: YM
authors.lastname: Dong
authors.forename: Neng-qin
authors.initials: NQ
authors.lastname: Jia
authors.forename: Hai-qing
authors.initials: HQ
authors.lastname: Zhou
authors.forename: Xi
authors.initials: X
authors.lastname: Xin
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D005290:Ferric Compounds / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000473:pathology; D008280:Magnetics; D020527:Microscopy, Scanning Probe / Q000295:instrumentation* / Q000379:methods; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; D016376:Oligonucleotides, Antisense; C000499:ferric oxide; D012822:Silicon Dioxide