Safety limit of the extent of hepatectomy for rats with moderately fatty liver: experimental study concerning living liver donor safety.
BACKGROUND
The aim of the present study was to determine whether rats with moderately fatty liver could withstand a 90% hepatectomy, which rats with normal livers can survive.


METHODS
Nine-week-old male Wistar rats were used. Normal rat chow was fed to the normal liver group, and fat-enriched rat chow was fed to the fatty liver group for 4 weeks to induce a moderately fatty liver. The authors have previously reported that this fatty liver rat model can cause fatal liver dysfunction after reduced-size-liver transplantation. A 90% and 95% hepatectomy were performed using rats of both groups to evaluate posthepatectomized liver function.


RESULTS
All rats undergoing a 90% hepatectomy were still alive 30 days after the hepatectomy, but the rats that underwent a 95% hepatectomy were all dead within 4 days regardless of group. Increases in the liver remnant wet weight measured until 7 postoperative days after 90% hepatectomy were almost similar among the two groups. Alanin aminotransferase measured at 24, 48, 72, and 168 h after a 90% hepatectomy were significantly higher in the fatty liver group than in the normal liver group. Similarly, at up to 72 h postoperatively, the serum hyarulonic acids were significantly higher in the fatty liver group.


CONCLUSIONS
A moderately fatty liver did not cause mortality in 90% hepatectomized rats. However, it caused a higher degree of hepatic parenchymal as well as sinusoidal injury.
Morioka
Daisuke
D
Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Fukuura, Kanazawa, Yokohama, Japan. dmorioka@hotmail.com
Watanabe
Kazuteru
K
Makino
Hirochika
H
Saito
Shuji
S
Ueda
Michio
M
Kubota
Toru
T
Sekido
Hitoshi
H
Matsuo
Ken-Ichi
K
Ichikawa
Yasushi
Y
Endo
Itaru
I
Togo
Shinji
S
Shimada
Hiroshi
H
D000818:Animals; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000097:blood / Q000401:mortality / Q000601:surgery*; D005500:Follow-Up Studies; D006498:Hepatectomy / Q000379:methods* / Q000401:mortality; D006820:Hyaluronic Acid / Q000097:blood; D016031:Liver Transplantation / Q000379:methods*; D019520:Living Donors; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015996:Survival Rate; D000637:Transaminases / Q000097:blood; D016896:Treatment Outcome
D015415:Biomarkers; D006820:Hyaluronic Acid; D000637:Transaminases