title: Development of DARPP-32-positive parts of fetal pig ganglionic eminence and ventral mesencephalon in organotypic slice co-cultures.
abstract: Neurons from the fetal pig dopaminergic ventral mesencephalon (VM) and basal ganglia anlage (the ganglionic eminence) were co-cultured as organotypic slice cultures to study the development of the two interconnected brain areas. During a short developmental period (E35-E42), a groove separates the ganglionic eminence into a lateral and a medial part. This was used (a) to study the developmental expression of the striatal marker protein, dopamine and adenosine 3,5-monophosphate regulated phospho-protein (DARPP-32) in the two parts and (b) to compare innervations of the two parts by tyrosine hydroxylase (TH)-positive, dopaminergic fibers from co-cultured slices of the ventral mesencephalon. DARPP-32 expression was more extensive and dense in cultures of the lateral part of the striatal anlage than the medial part. The DARPP-32-positive areas moreover overlapped with areas rich in acetylcholine esterase (AChE) and were the preferred target areas for TH-positive fibers from the co-cultured VM.
authors.affiliation: Anatomy and Neurobiology, Institute of Medical Biology, SDU-Odense University, Winsløwparken 21, DK-5000 Odense C, Denmark. amdall@health.sdu.dk
authors.forename: Annette Møller
authors.initials: AM
authors.lastname: Dall
authors.forename: Jens
authors.initials: J
authors.lastname: Zimmer
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000196:embryology* / Q000378:metabolism*; D018920:Coculture Techniques / Q000379:methods; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32 / Q000096:biosynthesis*; D005260:Female; D047109:Fetal Development / Q000502:physiology; D005333:Fetus / Q000378:metabolism* / Q000502:physiology; D008636:Mesencephalon / Q000196:embryology* / Q000378:metabolism*; D009924:Organ Culture Techniques / Q000379:methods; D013552:Swine
chemical_list: D051937:Dopamine and cAMP-Regulated Phosphoprotein 32