Pre-treatment of high oil and grease pet food industrial wastewaters using immobilized lipase hydrolyzation.
Wastewaters generating from pet food industries contain high concentration of oil and grease (O&G), which is difficult to treat through conventional biological treatment systems. In this study, the hydrolysis of O&G originating from pet food industrial wastewater was evaluated. Candida rugosa lipase was immobilized in calcium alginate beads and applied in the hydrolysis experiment. Results showed that approximately 50% of the O&G was hydrolyzed due to the enzyme activity. A significant increment in COD and VFA production was also observed. The immobilized lipase activity was confirmed with p-nitrophenyl palmitate (pNPP) before and after O&G hydrolysis. During the 3-day experiment, approximately 65% of the beads were recovered and after the hydrolysis, approximately 70% of the enzyme activity remained in the beads. This study shows the potential of immobilized lipase as a pre-treatment step in biological treatment of pet food manufacturing wastewater.
Jeganathan
Jeganaesan
J
Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario N6A 5B9, Canada.
Bassi
Amarjeet
A
Nakhla
George
G
D000821:Animal Feed; D004800:Enzymes, Immobilized; D019649:Food Industry; D015386:Hazardous Substances; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007220:Industrial Waste; D008049:Lipase / Q000737:chemistry*; D009821:Oils; D010100:Oxygen / Q000737:chemistry; D010168:Palmitates / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013696:Temperature; D014865:Waste Disposal, Fluid / Q000379:methods
D004800:Enzymes, Immobilized; D015386:Hazardous Substances; D007220:Industrial Waste; D009821:Oils; D010168:Palmitates; D011506:Proteins; C050015:4-nitrophenyl palmitate; D008049:Lipase; D010100:Oxygen