[The inhibitive effect of COX-2 inhibitor NS-398 on cancer cell growth of human tongue squamous cell carcinoma].
OBJECTIVE
To investigate the inhibitive effect of COX-2 inhibitor NS-398 on cancer cell growth of human tongue squamous cell carcinoma.


METHODS
MTT assay was used to study the inhibitive effect of NS-398 on the growth of Tca8113 cells, and to investigate the time-effect relationship and dose-effect relationship between NS-398 and Tca8113 cells. The effect of NS-398 on cell cycle changes of Tca8113 was studied by flow cytometry (FCM). The statistical date were analysed by SAS 8.1 software. Student's t test and repeated-measure ANOVA were used to analyze the date.


RESULTS
The Tca8113 cell culture median contained 0, 25, 50, 75, 100, 125, 150, 200 micromol/L NS-398, and individually cultured for 48, 72, 96, 120 hours. The inhibitive effect increased with the time prolonging and the concentration increasing. The effect was most obvious with 150 micromol/L NS-398 cultured for 96 hours (concentration: P<0.05, time: P<0.001). NS-398 can cause great increase of Tca8113 cells in G0/G1 phase, while cells in G2/M phase decreased, and cells arrested in G0/G1 phase (P<0.001).


CONCLUSIONS
NS-398 can inhibit the growth of Tca8113 cells. The inhibitive effect of NS-398 on Tca8113 cells is dose-dependent and time-dependent, and this effect may be related to the inhibition of cell circle growth. NS-398 may play an important role in the treatment of oral squamous cell carcinoma.
Sun
Xiao-ju
XJ
School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China.
Wang
Xu-kai
XK
Jia
Lan-ling
LL
D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D006801:Humans; D009578:Nitrobenzenes / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*; D014062:Tongue Neoplasms / Q000473:pathology*
D052246:Cyclooxygenase 2 Inhibitors; D009578:Nitrobenzenes; D013449:Sulfonamides; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide