title: Sensitivity of canine oocytes to low temperature.
abstract: This study compared the viability of canine oocytes after storage for 5 h at 4 or 38 degrees C. The ovaries were collected after ovariohysterectomy of bitches and transported to the laboratory within 5 h at 4 or 38 degrees C. The collected oocytes were matured in DMEM supplemented with 10% FBS, 0.6 mM/mL cysteine, 0.2 mM pyruvic acid, 20 ng/mL E2 and 1 microg/mL rbST, and incubated for 0, 24 and 48 h, at 38 degrees C and in 95% air with 5% CO2. The viability of the oocytes after 0 h did not differ significantly between 4 and 38 degrees C group (79.6% versus 83.9%), but after 24 and 48 h, significant differences were apparent (13.2% versus 77.8% after 24 h and 0.0% versus 72.9% after 48 h; P < 0.05). Therefore, canine oocytes were remarkably sensitive to low temperatures.
authors.affiliation: Department of Animal Science and Technology, Sunchon National University, 315 Maegok-dong, Sunchon, JeonNam Province 540-742, South Korea.
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.forename: X J
authors.initials: XJ
authors.lastname: Yin
authors.forename: I K
authors.initials: IK
authors.lastname: Kong
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000502:physiology; D003080:Cold Temperature; D004285:Dogs / Q000502:physiology*; D005260:Female; D008856:Microscopy, Fluorescence / Q000662:veterinary; D009865:Oocytes / Q000166:cytology / Q000502:physiology*