title: Effective proliferation of low level Legionella pneumophila serogroup 1 cells using coculture procedure with Acanthamoeba castellanii.
abstract: The multiplications of low level Legionella pneumophila serogroup 1 cells by the coculture procedure with Acanthamoeba castellanii were tested in five strains. The cells in all strains proliferated effectively for isolating. This procedure might be a useful means of improving the successful isolation from environmental and clinical specimens of low level Legionella cells, and pursuing the source of infection.
authors.affiliation: Division of 1st Microbiology, Hiroshima Prefectural Institute of Public Health and Environment, Minamimachi 1-6-29, Minami-ku, Hiroshima 734-0007, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Seno
authors.forename: Miyoko
authors.initials: M
authors.lastname: Sakaki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ogawa
authors.forename: Hanako
authors.initials: H
authors.lastname: Matsuda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Takeda
mesh_terms: D048248:Acanthamoeba castellanii / Q000254:growth & development*; D000818:Animals; D018920:Coculture Techniques; D015169:Colony Count, Microbial; D016952:Legionella pneumophila / Q000254:growth & development*; D007877:Legionnaires' Disease / Q000517:prevention & control; D014871:Water Microbiology