ChemMatrix, a poly(ethylene glycol)-based support for the solid-phase synthesis of complex peptides.
CM (ChemMatrix) resin is a new, totally poly(ethylene glycol) (PEG)-based resin, made exclusively from primary ether bonds and, therefore, highly chemically stable. It exhibits good loading and is user-friendly because of its free-flowing form upon drying. It performs excellently for the preparation of hydrophobic, highly structured, and poly-Arg peptides, as compared to polystyrene (PS) resins. In the most striking example, stepwise solid-phase assembly of the highly complex beta-amyloid (1-42) peptide resulted in a crude material of 91% purity. In contrast, literature procedures using PS or PEG-PS-based resins for this peptide required convergent approaches, additional time-consuming steps, or both. In addition to the difficulties of its synthesis, characterization of the beta-amyloid (1-42) peptide as a monomer is also a challenge, and methods for characterization by HPLC and MALDI-TOF have also been developed.
García-Martín
Fayna
F
Barcelona Biomedical Research Institute, Barcelona Science Park, University of Barcelona, Josep Samitier 1, 08028-Barcelona, Spain.
Quintanar-Audelo
Martina
M
García-Ramos
Yésica
Y
Cruz
Luis J
LJ
Gravel
Catherine
C
Furic
Robert
R
Côté
Simon
S
Tulla-Puche
Judit
J
Albericio
Fernando
F
D016229:Amyloid beta-Peptides / Q000138:chemical synthesis*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D010446:Peptide Fragments / Q000138:chemical synthesis*; D010455:Peptides / Q000138:chemical synthesis*; D011092:Polyethylene Glycols; D012117:Resins, Synthetic; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013055:Spectrophotometry, Infrared
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D010455:Peptides; D012117:Resins, Synthetic; C075222:amyloid beta-protein (1-42); D011092:Polyethylene Glycols