title: The relationship of soil province to molecular characterization and phylogenetic analysis of Cryptosporidium parvum isolated from calves in Georgia.
abstract: Although Cryptosporidium spp. are found throughout the world and in multiple environmental conditions, few data are available that explore the possibility of an association between specific environmental parameters and the species or strain of Cryptosporidium. This study examines the potential association between a particular Cryptosporidium species/strain found in calves and soil provinces in Georgia, USA. Necropsy cases spanning the years 1996-2002 were tested. No significant differences (P=0.962, chi(2) test of homogeneity) between numbers of positive cases were noted among soil provinces. Phylogenetic analysis of the sequences for the PCR products revealed sequence similarity of the products with Cryptosporidium parvum strain C1. Although, clinical Cryptosporidiosis in calves was not found to be affected by soil province and may be caused by a single genotype, other genotypes may be responsible for subclinical infection and warrant further investigation.
authors.affiliation: Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, The University of Georgia, 43 Brighton Road, Tifton, GA 31793, USA. millerdl@uga.edu
authors.forename: Debra L
authors.initials: DL
authors.lastname: Miller
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Mauel
authors.forename: Alan
authors.initials: A
authors.lastname: Liggett
authors.forename: Murray E
authors.initials: ME
authors.lastname: Hines
authors.forename: Kendall S
authors.initials: KS
authors.lastname: Frazier
authors.forename: Mel
authors.initials: M
authors.lastname: Pence
authors.forename: Lisa
authors.initials: L
authors.lastname: Whittington
authors.forename: Charles A
authors.initials: CA
authors.lastname: Baldwin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002417:Cattle / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000145:classification* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000032:analysis*; D005243:Feces / Q000469:parasitology*; D005845:Georgia; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012987:Soil / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D012987:Soil