Differential expression of two class III chitinases in two types of roots of Quercus robur during pre-mycorrhizal interactions with Piloderma croceum.
Expression of two plant chitinase genes, representing members of class III chitinases, was studied in Quercus robur roots during interactions in a pre-mycorrhizal stage with the ectomycorrhizal fungus Piloderma croceum. Chitinase gene expression was compared in lateral roots destined to form ectomycorrhiza, and in principal roots that are not directly involved in mycorrhizal interactions. The transcript level of the first chitinase (QrchitIII-1) was upregulated in lateral roots, whereas no significant differential expression was observed in principal roots. The second chitinase (QrchitIII-2) was regulated neither in lateral nor in principal roots in presence of the fungus. Because P. croceum did not induce significant chitinase responses in principal roots, the enhanced expression of QrchitIII-1 in lateral roots after inoculation may be related to some steps in symbiosis ontogenesis.
Frettinger
Patrick
P
Department of Terrestrial Ecology, Institute of Botany I, University of Leipzig, Johannisallee 21-23, 04103, Leipzig, Germany.|"Tree-Microbe Interactions Unit", Institut National de la Recherche Agronomique, Champenoux, 54280, France.|Institute of General Botany and Plant Physiology, University of Jena, Dornburger Straβe 159, 07743, Jena, Germany.
Herrmann
Sylvie
S
Department of Terrestrial Ecology, Institute of Botany I, University of Leipzig, Johannisallee 21-23, 04103, Leipzig, Germany.|Institute of Ecology, University of Jena, Dornburger Straβe 159, 07743, Jena, Germany.
Lapeyrie
Frédéric
F
"Tree-Microbe Interactions Unit", Institut National de la Recherche Agronomique, Champenoux, 54280, France.
Oelmüller
Ralf
R
Institute of General Botany and Plant Physiology, University of Jena, Dornburger Straβe 159, 07743, Jena, Germany.
Buscot
François
F
Department of Terrestrial Ecology, Institute of Botany I, University of Leipzig, Johannisallee 21-23, 04103, Leipzig, Germany. francois.buscot@ufz.de.|Center for Environmental Research (UFZ), Department for Soil Ecology, Theodor-Lieser-Straβe 4, 06120, Halle, Germany. francois.buscot@ufz.de.
D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D038821:Mycorrhizae / Q000502:physiology*; D010940:Plant Proteins; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000382:microbiology; D029963:Quercus / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D013559:Symbiosis
D010940:Plant Proteins; D002688:Chitinases; C110892:chitinase, class III