High-resolution imaging of progressive articular cartilage degeneration.
The objective of this study was to develop and verify a new technique for monitoring the progression of osteoarthritis (OA) by combining a rat model with the imaging modality optical coherence tomography (OCT). Time-sequential, in vivo, OCT imaging was performed on the left femoral condyles of 12 Wistar rats following sodium-iodoacetic acid-induced OA progression. The right femoral condyles (untreated) were also imaged and served as controls. Imaging was performed on days 0, 10, 20, 30, and 60 with an OCT system capable of acquiring images at four frames per second and an axial resolution of 5 microm. Progressive changes were analyzed using an OA scoring system. OCT successfully identified progressive cartilage degeneration as well as alteration of the cartilage/bone interface. Significant changes to both of these structures were observed in the sodium-iodoacetic acid-injected condyles. Structural changes detected with OCT were confirmed histologically. OCT in combination with a well-known model used in arthritis research represents a powerful tool for following degenerative joint disease progression in a given animal by detecting changes to the cartilage/bone interface and articular cartilage.
Adams
Samuel B
SB
Department of Orthopedic Surgery, Brigham and Women's Hospital, 75 Francis Street, Boston, Massachusetts 02139, USA.
Herz
Paul R
PR
Stamper
Debra L
DL
Roberts
Mark J
MJ
Bourquin
Stephane
S
Patel
Nirlep A
NA
Schneider
Karl
K
Martin
Scott D
SD
Shortkroff
Sonya
S
Fujimoto
James G
JG
Brezinski
Mark E
ME
D000818:Animals; D002358:Cartilage, Articular / Q000473:pathology*; D004195:Disease Models, Animal; D007461:Iodoacetates; D008297:Male; D010003:Osteoarthritis / Q000175:diagnosis*; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D041623:Tomography, Optical Coherence / Q000379:methods*
D007461:Iodoacetates