Phytoextraction with Brassica napus L.: a tool for sustainable management of heavy metal contaminated soils.
Phytoextraction is a promising tool to extract metals from contaminated soils and Brassica napus L. seems to be a possible candidate species for this purpose. To select accessions with the ability to accumulate cadmium, hydroponically grown 21 day old seedlings of 77 B. napus L. accessions were exposed to 0.2 microM CdSO(4) for an additional 10 days. The effects of Cd on several parameters were quantified i.e.; shoot Cd concentration ([Cd](shoot)), total amount of Cd in shoots (Total Cd) and the shoot to root Cd concentration ratio (S/R ratio). Though generally natural variation was low for [Cd](shoot), Total Cd and S/R ratio, a number of accessions could be selected. Our results indicated that Total Cd and S/R ratio are independent parameters for Cd accumulation and translocation. The selected varieties were then tested in field experiments on two locations nearby metal smelters. The two locations differed in extractable soil Cd, Zn, Ca concentration and pH levels. On both locations B. napus L. accessions showed significant differences in [Cd](shoot) and Total Cd. Furthermore we found significant correlations between Cd and Zn accumulation in shoots. There were site-specific effects with respect to Cd accumulation in the B. napus L. accessions, however, two accessions seem to perform equally well on both sites. The results of the field experiment suggest that certain B. napus L. accessions are suitable for phytoextraction of moderately heavy metal contaminated soils.
Grispen
Veerle M J
VM
Department of Ecology and Physiology of Plants, Vrije Universiteit, De Boelelaan 1085, NL-1081 HV Amsterdam, The Netherlands.
Nelissen
Hans J M
HJ
Verkleij
Jos A C
JA
D001530:Belgium; D001673:Biodegradation, Environmental; D001937:Brassica / Q000235:genetics / Q000378:metabolism*; D002104:Cadmium / Q000032:analysis; D018527:Hydroponics; D007220:Industrial Waste; D008669:Metallurgy; D019216:Metals, Heavy / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry; D012987:Soil / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis; D015032:Zinc / Q000032:analysis
D007220:Industrial Waste; D019216:Metals, Heavy; D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium; D015032:Zinc