[Study on vascular endothelial injuries and inflammation related cytokines in patients with multiple organ dysfunction syndrome].
OBJECTIVE
To investigate the relationship between the circulating endothelial cell (CEC), coagulation, fibrinolysis indexes and inflammation related cytokines in patients with multiple organ dysfunction syndrome (MODS).


METHODS
Thirty-five patients with MODS (MODS group) and 20 healthy individuals (control group) were enrolled for study. CECs were harvested by isopycnic centrifugation method. Plasma prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FG), D-dimer, and serum tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) were determined simultaneously. The relationship between dynamic changes in CEC, TNF-alpha, IL-6 and that in PT, APTT, FG, D-dimer was analyzed.


RESULTS
Compared to the control group, CEC numbers, serum TNF-alpha, IL-6 levels and plasma D-dimer level significantly increased, PT and APTT were prolonged, and FG significantly decreased in MODS group (all P<0.05). The quantity of CEC and serum levels of TNF-alpha and IL-6 elevated with the lapse of time. Correlation analysis revealed a significant positive correlation between CEC and serum levels of TNF-alpha and IL-6 (r(1)=0.536, P(1)=0.000; r(2)=0.412, P(2)=0.002), and the same correlation between serum TNF-alpha, IL-6 levels and PT, APTT.


CONCLUSIONS
Elevation of serum inflammation related cytokines and vascular endothelial cell damage occur in MODS patients. Serum inflammation related cytokines play an important role in endothelial damage, and an increase in CEC number may be attributed to vessel injury.
Wang
Jing
J
Department of Emergency, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, Shanxi, China. wangjinghehe@sina.com
Qiao
Wan-hai
WH
D000328:Adult; D016207:Cytokines / Q000097:blood*; D042783:Endothelial Cells / Q000473:pathology*; D004730:Endothelium, Vascular / Q000473:pathology*; D005260:Female; D005340:Fibrinogen; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000097:blood* / Q000473:pathology; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D005340:Fibrinogen