Acceleration of de novo bone formation following application of autogenous bone to particulated anorganic bovine material in vivo.
OBJECTIVE
This prospective animal study examined the de novo bone formation following application of deproteinized bovine bone matrix (DBBM) with or without autogenous bone (AB) to osseous defects.


METHODS
Defects of defined size were created in the frontal skull of domestic pigs and filled with DBBM alone (group A) and DBBM+25%AB (group B). De novo bone formation was analyzed qualitatively and quantitatively at 9 different times (0.5, 1, 2, 3, 4, 6, 8, 12, 26 weeks) by means of light microscopy, microradiography, and statistical analysis.


RESULTS
Histological analysis indicated sufficient osseointegration of DBBM in both groups. Microradiography demonstrated a significant increase of bone formation in group B after 6 weeks (P = .0159) and 8 weeks (P = .0317).


CONCLUSIONS
The addition of 25%AB to DBBM results in accelerated de novo bone formation in osseous defects. This effect is likely caused by osteoinductive properties of cellular elements transplanted with the autogenous bone.
Thorwarth
Michael
M
Department of Oral and Maxillofacial Surgery/Plastic Surgery, University of Jena, Jena, Germany. Michael.Thorwarth@med.uni-jena.de
Schlegel
Karl Andreas
KA
Wehrhan
Falk
F
Srour
Safwan
S
Schultze-Mosgau
Stefan
S
D000818:Animals; D001857:Bone Matrix / Q000637:transplantation*; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology*; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D005260:Female; D008852:Microradiography; D008903:Minerals / Q000494:pharmacology*; D011446:Prospective Studies; D012886:Skull / Q000601:surgery; D018709:Statistics, Nonparametric; D034421:Sus scrofa; D013552:Swine; D014182:Transplantation, Autologous
C077540:Bio-Oss; D018786:Bone Substitutes; D008903:Minerals