title: Autotrophic growth of Acidithiobacillus ferrooxidans by oxidation of molecular hydrogen using a gas-liquid contactor.
abstract: The iron-oxidizing bacterium, Acidithiobacillus ferrooxidans, was cultivated on a medium without ferrous iron. Molecular hydrogen and air were supplied to the medium. It was found that A. ferrooxidans could grow with hydrogen in the pH range between 2.0 and 3.5. A trickle-bed contactor was used to increase the dissolution rate of hydrogen. The doubling time was increased and the cell concentration reached 4.0 x 10(9) cells ml(-1) after 6 days. When the cells taken from the hydrogen medium were transferred back into the medium containing ferrous iron, the growth rate and the iron-oxidizing ability were the same as the predictions assuming that the microorganism grown with hydrogen was A. ferrooxidans.
authors.affiliation: Department of Applied Chemistry and Chemical Engineering, Kagoshima University, Kagoshima 890-0065, Japan. kai@cen.kagoshima-u.ac.jp
authors.forename: Takami
authors.initials: T
authors.lastname: Kai
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Nagano
authors.forename: Tomonori
authors.initials: T
authors.lastname: Fukumoto
authors.forename: Masaya
authors.initials: M
authors.lastname: Nakajima
authors.forename: Takeshige
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D019149:Bioreactors; D020563:Gammaproteobacteria / Q000254:growth & development* / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction
chemical_list: D006859:Hydrogen