Crystal structure of non-fused glutathione S-transferase from Schistosoma japonicum in complex with glutathione.
Schistosoma japonicum glutathione S-transferase (SjGST) is a common fusion tag in recombinant protein production, and its 3-dimensional structure has been studied in the context of drug design. We have determined the crystal structure of non-fused SjGST complexed with glutathione, and compare it to complexes between glutathione and SjGST fusion proteins.
Kursula
I
I
Department of Biochemistry, Biocenter Oulu, Oulu University, Oulu, Finland.
Heape
A M
AM
Kursula
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P
D000818:Animals; D018360:Crystallography, X-Ray; D005978:Glutathione / Q000737:chemistry* / Q000378:metabolism*; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012549:Schistosoma japonicum / Q000201:enzymology* / Q000235:genetics
D011994:Recombinant Proteins; D005982:Glutathione Transferase; D005978:Glutathione