The carboxyl-terminal domain of large T antigen rescues SV40 host range activity in trans independent of acetylation.
The host range activity of SV40 has been described as the inability of mutant viruses with deletions in the C terminal region of large T Ag to replicate in certain types of African green monkey kidney cells. We constructed new mutant viruses expressing truncated T Ag proteins and found that these mutant viruses exhibited the host range phenotype. The host range phenotype was independent of acetylation of T Ag at lysine 697. Co-expression of the C terminal domain of T Ag (aa 627-708) in trans increased both T Ag and VP1 mRNA as well as protein levels for host range mutant viruses in the restrictive cell type. In addition, the T Ag 627-708 fragment promoted the productive lytic infection of host range mutant viruses in the nonpermissive cell type. The carboxyl-terminal region of T Ag contains a biological function essential for the SV40 viral life cycle.
Poulin
Danielle L
DL
Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
DeCaprio
James A
JA
D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D036022:Capsid Proteins / Q000032:analysis; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D002522:Chlorocebus aethiops; D015870:Gene Expression; D005816:Genetic Complementation Test; D008239:Lysine / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D017384:Sequence Deletion; D013539:Simian virus 40 / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D010948:Viral Plaque Assay
D000952:Antigens, Polyomavirus Transforming; D036022:Capsid Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; C062481:VP1 protein, polyomavirus; D008239:Lysine