Spectrophotometric determination of anthracycline anticancer agents with aluminum(III) and chromazurol S in a nonionic surfactant micellar medium.
A simple and highly sensitive spectrophotometric method for the determination of anthracycline anticancer agents, such as Daunorubicin hydrochloride (DAU), was established by using aluminum(III) and Chromazurol S (CAS) in a nonionic surfactant micellar medium. In the case of determination of DAU, the apparent molar absorptivity was 1.3 x 10(5) dm3 mol(-1) cm(-1) at 615 nm. Beer's law was obeyed in the concentration range of 0.028 - 2.82 microg ml(-1) for DAU. Owing to no need for solvent extraction, this method could be applied to assays of DAU and related drugs in pharmaceutical preparations.
Nakao
Masahiro
M
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. m04927@gly.oups.ac.jp
Yamazaki
Chikako
C
Tominaga
Hiroshi
H
Yamaguchi
Takako
T
Fujita
Yoshikazu
Y
D000535:Aluminum / Q000737:chemistry*; D018943:Anthracyclines / Q000737:chemistry*; D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004396:Coloring Agents / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D062385:Hydroxybenzoates / Q000737:chemistry*; D007477:Ions; D008823:Micelles; D012044:Regression Analysis; D013053:Spectrophotometry; D013501:Surface-Active Agents / Q000737:chemistry
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; D000970:Antineoplastic Agents; D004396:Coloring Agents; D062385:Hydroxybenzoates; D007477:Ions; D008823:Micelles; D013501:Surface-Active Agents; C015076:chrome azurol S; D000535:Aluminum