Overexpression of PRV-1 gene in polycythemia rubra vera and essential thrombocythemia.
The polycythemia rubra vera 1 gene (PRV-1), a member of the urokinase-type plasminogen activator receptor superfamily, is overexpressed in granulocytes isolated from the peripheral blood of patients with polycythemia vera (PV) and essential thrombocythemia (ET). PRV-1 overexpression is the first reliable molecular marker of these myeloproliferative disorders, and its detection allows us to discriminate PV and ET from secondary erythrocytosis and thrombocytosis. PRV-1 overexpression can be investigated by several techniques, including Northern analysis, reverse-transcription (RT)-polymerase chain reaction (PCR), and real-time PCR. Among these, RT-PCR is the most rapid, reliable, and feasible method for the detection of PRV-1 overexpression in highly purified peripheral blood granulocytes.
Martini
Maurizio
M
Instituto di Anatomia Patologica and Ematologia, Università Cattalica del Sacro Cuore, Roma, Italy.
Teofili
Luciana
L
Larocca
Luigi M
LM
D003937:Diagnosis, Differential; D058851:GPI-Linked Proteins; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000502:physiology; D006801:Humans; D007519:Isoantigens / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics*; D011087:Polycythemia Vera / Q000175:diagnosis / Q000235:genetics*; D012313:RNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013920:Thrombocythemia, Essential / Q000175:diagnosis / Q000235:genetics*
C407242:CD177 protein, human; D058851:GPI-Linked Proteins; D007519:Isoantigens; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D012313:RNA