Immobilization of pancreatic lipase on chitin and chitosan.
In this study, porcine pancreatic lipase (EC 3.1.1.3) was immobilized on chitin and chitosan by adsorption and subsequent crosslinking with glutaraldehyde, which was added before (conjugation) or after (crosslinking) washing unbound proteins. Conjugation proved to be the better method for both supports. The properties of free and immobilized enzymes were also investigated and compared. The results showed that the pH optimum was shifted from 8.5 to 9.0 for both the immobilized enzymes. Also, the optimum temperature was shifted from 30 to 40 degrees C for chitin-enzyme and to 45 degrees C for chitosan-enzyme conjugates. The immobilization efficiency is low, but the immobilized enzymes have good reusability and stability (storage and operational). Besides these properties, the immobilized lipases were also suitable for catalyzing esterification reactions of fatty acids and fatty alcohols, both with a medium chain length. According to our results, esterification activities of immobilized lipases were two- and four-fold higher for chitosan- and chitin-enzyme, than for the free enzyme, respectively. The immobilization procedure shows a great potential for commercial applications of the immobilized lipase, a relatively low cost commercial enzyme.
Kilinç
Ali
A
Department of Biochemistry, Faculty of Science, Ege University, Bornova-Izmir, Turkey. ali.kilinc@ege.edu.tr
Teke
Mustafa
M
Onal
Seçil
S
Telefoncu
Azmi
A
D000818:Animals; D002686:Chitin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D020799:Pancrelipase / Q000737:chemistry*; D013379:Substrate Specificity; D013552:Swine; D013696:Temperature
D004800:Enzymes, Immobilized; D002686:Chitin; D020799:Pancrelipase; D048271:Chitosan