title: [Isolation, identification and functional characterization of human CD4+ CD25+ Treg cells from human peripheral blood].
abstract: OBJECTIVE
  To isolate CD4(+) CD25(+) Treg cells from human peripheral blood, and study their functional characteristics.
  
  
  METHODS
  The expressions of Foxp3 in human CD4(+) CD25(+) Treg cells were test by RT-PCR. The regulatory properties of CD4(+) CD25(+) Treg cells were assessed by co-culturing with CD4(+) CD25(-) T cells and CD8(+) T cells, or adding exogenous IL-2. The detection of intracellular cytokine production of IL-4, IL-10 and IFN-gamma was done by flow cytometry.
  
  
  RESULTS
  CD4(+) CD25(+) Treg cells highly expressed Foxp3 and mainly synthesized IL-10 that suppressed the proliferation of CD4(+) CD25(-) T and CD8(+) T cells. Its suppressive function was reversed by high concentration of IL-2 and (or) IL-4.
  
  
  CONCLUSIONS
  CD4(+) CD25(+) Treg cells exhibited a subpopulation of immunoregulatory T cells with suppressive function, which could be reversed by high concentration of IL-2.
authors.affiliation: Department of Infectious Diseases, the First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China. yjhpath@163.com
authors.forename: Jiang-hua
authors.initials: JH
authors.lastname: Yang
authors.forename: Yong-xiang
authors.initials: YX
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Rong-bin
authors.initials: RB
authors.lastname: Yu
authors.forename: Chuan
authors.initials: C
authors.lastname: Su
authors.forename: Nan-xiong
authors.initials: NX
authors.lastname: Sun
mesh_terms: D001773:Blood Cells / Q000145:classification* / Q000502:physiology; D015704:CD4 Antigens / Q000302:isolation & purification* / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000302:isolation & purification* / Q000502:physiology; D050378:T-Lymphocytes, Regulatory / Q000145:classification* / Q000502:physiology
chemical_list: D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit