title: Structure of Lmaj006129AAA, a hypothetical protein from Leishmania major.
abstract: The gene product of structural genomics target Lmaj006129 from Leishmania major codes for a 164-residue protein of unknown function. When SeMet expression of the full-length gene product failed, several truncation variants were created with the aid of Ginzu, a domain-prediction method. 11 truncations were selected for expression, purification and crystallization based upon secondary-structure elements and disorder. The structure of one of these variants, Lmaj006129AAH, was solved by multiple-wavelength anomalous diffraction (MAD) using ELVES, an automatic protein crystal structure-determination system. This model was then successfully used as a molecular-replacement probe for the parent full-length target, Lmaj006129AAA. The final structure of Lmaj006129AAA was refined to an R value of 0.185 (Rfree = 0.229) at 1.60 A resolution. Structure and sequence comparisons based on Lmaj006129AAA suggest that proteins belonging to Pfam sequence families PF04543 and PF01878 may share a common ligand-binding motif.
authors.affiliation: Department of Biochemistry, University of Washington, Seattle, WA 98195-7742, USA.
authors.forename: Tracy
authors.initials: T
authors.lastname: Arakaki
authors.forename: Isolde
authors.initials: I
authors.lastname: Le Trong
authors.forename: Eric
authors.initials: E
authors.lastname: Phizicky
authors.forename: Erin
authors.initials: E
authors.lastname: Quartley
authors.forename: George
authors.initials: G
authors.lastname: DeTitta
authors.forename: Joseph
authors.initials: J
authors.lastname: Luft
authors.forename: Angela
authors.initials: A
authors.lastname: Lauricella
authors.forename: Lori
authors.initials: L
authors.lastname: Anderson
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Kalyuzhniy
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Worthey
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Myler
authors.forename: David
authors.initials: D
authors.lastname: Kim
authors.forename: David
authors.initials: D
authors.lastname: Baker
authors.forename: Wim G J
authors.initials: WG
authors.lastname: Hol
authors.forename: Ethan A
authors.initials: EA
authors.lastname: Merritt
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D018320:Leishmania major / Q000737:chemistry*; D015800:Protozoan Proteins / Q000737:chemistry*
chemical_list: D015800:Protozoan Proteins