Immobilization and enzymatic activity of glucose oxidase on polystyrene surface modified with ozone aeration and UV irradiation in distilled water and/or aqueous ammonia solution.
Adsorption condition and enzymatic activity of glucose oxidase (GOD) on polystyrene (PS) film surfaces modified with ozone aeration and UV irradiation (O3/UV) treatment were investigated. The total amount of GOD immobilized on the PS film modified with the O3/UV treatment in distilled water (PS-W film) was approximately twice as large as that on the film treated in an aqueous ammonia solution (PS-A film), whereas the specific activity of GOD on the PS-A film was four times higher than that on the PS-W film. In contrast, no enzymatic activity of GOD on the non-treated PS film was observed because of irreversible denaturation of the adsorbed GOD. We therefore conclude that the PS films modified by the O3/UV treatment in the aqueous media are effective in immobilizing GOD.
Yanagisawa
Ken
K
Faculty of Biomedical Engineering, Toin University of Yokohama, 1614 Kurogane-cho, Aoba-ku, Yokohama 225-8502, Japan.
Murakami
Takurou N
TN
Tokuoka
Yoshikazu
Y
Ochiai
Akira
A
Takahashi
Mitsuo
M
Kawashima
Norimichi
N
D000327:Adsorption; D000641:Ammonia / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000493:pharmacokinetics / Q000528:radiation effects; D005949:Glucose Oxidase / Q000737:chemistry* / Q000493:pharmacokinetics / Q000528:radiation effects; D018625:Microscopy, Atomic Force; D010126:Ozone / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry*
D004800:Enzymes, Immobilized; D011137:Polystyrenes; D012996:Solutions; D014867:Water; D010126:Ozone; D000641:Ammonia; D005949:Glucose Oxidase