Crystallization of the C-terminal domain of the addiction antidote CcdA in complex with its toxin CcdB.
CcdA and CcdB are the antidote and toxin of the ccd addiction module of Escherichia coli plasmid F. The CcdA C-terminal domain (CcdAC36; 36 amino acids) was crystallized in complex with CcdB (dimer of 2 x 101 amino acids) in three different crystal forms, two of which diffract to high resolution. Form II belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 37.6, b = 60.5, c = 83.8 A and diffracts to 1.8 A resolution. Form III belongs to space group P2(1), with unit-cell parameters a = 41.0, b = 37.9, c = 69.6 A, beta = 96.9 degrees, and diffracts to 1.9 A resolution.
Buts
Lieven
L
Department of Molecular and Cellular Interactions, Vlaams Interinuversitair Instituut voor Biotechnologie and Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
De Jonge
Natalie
N
Loris
Remy
R
Wyns
Lode
L
Dao-Thi
Minh-Hoa
MH
D001426:Bacterial Proteins / Q000737:chemistry*; D001427:Bacterial Toxins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D019143:Evolution, Molecular; D008565:Membrane Proteins / Q000737:chemistry*; D015233:Models, Statistical; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D014118:Toxins, Biological / Q000737:chemistry; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C104882:CcdA protein, Bacillus; C068356:CcdB protein, Plasmid F; D008565:Membrane Proteins; D014118:Toxins, Biological