title: Metabolic characterization of the R6/2 transgenic mouse model of Huntington's disease by high-resolution MAS 1H NMR spectroscopy.
abstract: The metabolic consequences of Huntington's disease in the R6/2 mouse model were investigated using NMR spectroscopy and pattern recognition to characterize selected brain regions, muscle, blood, and urine. Global increases in relative brain concentrations of osmolytes, creatine, glutamine, and lactate, and decreases in acetate and N-acetylaspartate were found together with striatal-specific lower concentrations of GABA and choline. Clear differentiation of R6/2 and wild-type mice was also obtained for urine and blood metabolite profiles that may have applicability for monitoring HD in human populations.
authors.affiliation: Biological Chemistry, Biomedical Sciences Division, Faculty of Medicine, Imperial College, London, SW7 2AZ, United Kingdom.
authors.forename: Tsz M
authors.initials: TM
authors.lastname: Tsang
authors.forename: Ben
authors.initials: B
authors.lastname: Woodman
authors.forename: Gerard A
authors.initials: GA
authors.lastname: McLoughlin
authors.forename: Julian L
authors.initials: JL
authors.lastname: Griffin
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Tabrizi
authors.forename: Gillian P
authors.initials: GP
authors.lastname: Bates
authors.forename: Elaine
authors.initials: E
authors.lastname: Holmes
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001923:Brain Chemistry; D004195:Disease Models, Animal; D005260:Female; D006816:Huntington Disease / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D008137:Longitudinal Studies; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D015415:Biomarkers