title: Protective effect of dietary long-chain n-3 polyunsaturated fatty acids on bone loss in gonad-intact middle-aged male rats.
abstract: This study evaluated the effect of a fat blend containing long-chain (LC) n-3 PUFA on bone mineral density (BMD) and bone metabolism in gonad-intact middle-aged male rats (12 months old, n 28). Seven rats were killed on day 0 of dietary intervention to determine the baseline BMD. The remaining rats (seven per group) were fed a diet with one of the following dietary lipid treatments (g/kg diet): 167 g safflower oil + 33 g menhaden oil (N6 + N3 diet, control), 200 g safflower oil (N6 diet, almost devoid of LC n-3 PUFA), or 190 g menhaden oil + 10 g corn oil (N3 diet, rich in LC n-3 PUFA) for 20 weeks. After 20 weeks, all dietary treatment groups had a lower BMD compared with the baseline reference. However, rats fed the N3 diet had the highest bone mineral content and cortical + subcortical BMD compared with those fed the N6 and control N6 + N3 diet. Compared with the control (N6 + N3) group, rats fed the N3 diet had higher values for serum insulin-like growth factor-I, parathyroid hormone, 1,25-(OH)2 vitamin D3 and bone-specific alkaline phosphatase activity, but lower bone NO production and urinary Ca, whereas rats fed the N6 diet had higher bone prostaglandin E2 production and serum pyridinoline. These findings indicate a protective action of LC n-3 PUFA on ageing-induced bone loss in gonad-intact middle-aged male rats through a modulation of local factors and systemic calcitrophic hormones.
authors.affiliation: Department of Pathology, Texas Tech University Health Sciences Center, Lubbock, USA. Leslie.Shen@ttuhsc.edu
authors.forename: Chwan-Li
authors.initials: CL
authors.lastname: Shen
authors.forename: James K
authors.initials: JK
authors.lastname: Yeh
authors.forename: Jahan
authors.initials: J
authors.lastname: Rasty
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Watkins
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000596:Amino Acids / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D015519:Bone Density / Q000187:drug effects* / Q000502:physiology; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis; D005227:Fatty Acids / Q000032:analysis; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis; D010710:Phosphates / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D014807:Vitamin D / Q000097:blood
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D010710:Phosphates; D014807:Vitamin D; D009569:Nitric Oxide; C015484:pyridinoline; D007334:Insulin-Like Growth Factor I; D000469:Alkaline Phosphatase; D015232:Dinoprostone; D002118:Calcium