title: Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis.
abstract: Protein structures from the causative agent of anthrax (Bacillus anthracis) are being determined as part of a structural genomics programme. Amongst initial candidates for crystallographic analysis are enzymes involved in nucleotide biosynthesis, since these are recognized as potential targets in antibacterial therapy. Purine nucleoside phosphorylase is a key enzyme in the purine-salvage pathway. The crystal structure of purine nucleoside phosphorylase (DeoD) from B. anthracis has been solved by molecular replacement at 2.24 A resolution and refined to an R factor of 18.4%. This is the first report of a DeoD structure from a Gram-positive bacterium.
authors.affiliation: Department of Chemistry, University of York, York YO10 5YW, England.
authors.forename: Rosa
authors.initials: R
authors.lastname: Grenha
authors.forename: Vladimir M
authors.initials: VM
authors.lastname: Levdikov
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Fogg
authors.forename: Elena V
authors.initials: EV
authors.lastname: Blagova
authors.forename: James A
authors.initials: JA
authors.lastname: Brannigan
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Wilkinson
authors.forename: Keith S
authors.initials: KS
authors.lastname: Wilson
mesh_terms: D000595:Amino Acid Sequence; D001408:Bacillus anthracis / Q000201:enzymology* / Q000378:metabolism; D019976:Cloning, Organism; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011683:Purine-Nucleoside Phosphorylase / Q000737:chemistry* / Q000378:metabolism; D016415:Sequence Alignment; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D011683:Purine-Nucleoside Phosphorylase