title: Presence of mitomycin-C in the anterior chamber after photorefractive keratectomy.
abstract: OBJECTIVE
  To assess the presence of mitomycin-C (MMC) in hen aqueous humor after photorefractive keratectomy (PRK).
  
  
  METHODS
  Instituto Universitario de Oftalmobiología Aplicada, Faculty of Medicine, University of Valladolid, and Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Valladolid, Spain.
  
  
  METHODS
  Mitomycin-C 0.02% was applied topically for 2 minutes to a right hen's eye after PRK (Group A) and to the left eye with intact epithelium (Group B). At different time points (10, 30, 60, 360, and 720 minutes), aqueous humor was extracted and high-performance liquid chromatography was performed to detect and quantify MMC levels.
  
  
  RESULTS
  The mean maximum drug concentration of MMC measured in the aqueous humor was 187.250 microg/L +/- 4.349 (SD) in Group A and 93.000 +/- 4.899 microg/L in Group B, both detected 10 minutes after topical application. Statistically significant differences were found between Groups A and B at 10, 30, and 60 minutes, with decreasing MMC levels in both groups but a higher concentration in Group A. After 360 minutes, MMC levels were undetectable in Group B and after 720 minutes in Group A.
  
  
  CONCLUSIONS
  Mitomycin-C was detectable in the aqueous humor of the hen eye after topical application in PRK-treated eyes and in eyes with intact epithelium. The presence of MMC is of concern as it may lead to ocular toxicity in the long term.
authors.affiliation: Instituto Universitario de Oftalmobiología Aplicada, Universidad de Valladolid, Spain.
authors.forename: Rodrigo M
authors.initials: RM
authors.lastname: Torres
authors.forename: Jesús
authors.initials: J
authors.lastname: Merayo-Lloves
authors.forename: Sheraz M
authors.initials: SM
authors.lastname: Daya
authors.forename: José T
authors.initials: JT
authors.lastname: Blanco-Mezquita
authors.forename: Marcela
authors.initials: M
authors.lastname: Espinosa
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Nozal
authors.forename: José L
authors.initials: JL
authors.lastname: Bernal
authors.forename: José
authors.initials: J
authors.lastname: Bernal
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics*; D001082:Aqueous Humor / Q000378:metabolism*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003315:Cornea / Q000378:metabolism* / Q000601:surgery; D054018:Lasers, Excimer; D016685:Mitomycin / Q000493:pharmacokinetics*; D018903:Photorefractive Keratectomy
chemical_list: D000903:Antibiotics, Antineoplastic; D016685:Mitomycin