Landscape of the hnRNP K protein-protein interactome.
The heterogeneous nuclear ribonucleoprotein K is an ancient RNA/DNA-binding protein that is involved in multiple processes that compose gene expression. The pleiotropic action of K protein reflects its ability to interact with different classes of factors, interactions that are regulated by extracellular signals. We used affinity purification and MS to better define the repertoire of K protein partners. We identified a large number of new K protein partners, some typically found in subcellular compartments, such as plasma membrane, where K protein has not previously been seen. Electron microscopy showed K protein in the nucleus, cytoplasm, mitochondria, and in vicinity of plasma membrane. These observations greatly expanded the view of the landscape of K protein-protein interaction and provide new opportunities to explore signal transduction and gene expression in several subcellular compartments.
Mikula
Michał
M
Department of Gastroenterology, Medical Center for Postgraduate Education and Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland.
Dzwonek
Artur
A
Karczmarski
Jakub
J
Rubel
Tymon
T
Dadlez
Michał
M
Wyrwicz
Lucjan S
LS
Bomsztyk
Karol
K
Ostrowski
Jerzy
J
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000737:chemistry*; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000473:pathology; D013058:Mass Spectrometry / Q000379:methods; D025941:Protein Interaction Mapping; D040901:Proteomics / Q000379:methods*; D051381:Rats; D015398:Signal Transduction; D013347:Subcellular Fractions
D034641:Heterogeneous-Nuclear Ribonucleoprotein K