title: Predicting the clinical behavior of ovarian cancer from gene expression profiles.
abstract: We investigated whether prognostic information is reflected in the expression patterns of ovarian carcinoma samples. RNA obtained from seven FIGO stage I without recurrence, seven platin-sensitive advanced-stage (III or IV), and six platin-resistant advanced-stage ovarian tumors was hybridized on a complementary DNA microarray with 21,372 spotted clones. The results revealed that a considerable number of genes exhibit nonaccidental differential expression between the different tumor classes. Principal component analysis reflected the differences between the three tumor classes and their order of transition. Using a leave-one-out approach together with least squares support vector machines, we obtained an estimated classification test accuracy of 100% for the distinction between stage I and advanced-stage disease and 76.92% for the distinction between platin-resistant versus platin-sensitive disease in FIGO stage III/IV. These results indicate that gene expression patterns could be useful in clinical management of ovarian cancer.
authors.affiliation: Department of Electrical Engineering ESAT-SCD, K.U.Leuven, Leuven-Heverlee, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: De Smet
authors.forename: N L M M
authors.initials: NL
authors.lastname: Pochet
authors.forename: K
authors.initials: K
authors.lastname: Engelen
authors.forename: T
authors.initials: T
authors.lastname: Van Gorp
authors.forename: P
authors.initials: P
authors.lastname: Van Hummelen
authors.forename: K
authors.initials: K
authors.lastname: Marchal
authors.forename: F
authors.initials: F
authors.lastname: Amant
authors.forename: D
authors.initials: D
authors.lastname: Timmerman
authors.forename: B L R
authors.initials: BL
authors.lastname: De Moor
authors.forename: I B
authors.initials: IB
authors.lastname: Vergote
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000627:therapeutic use; D019008:Drug Resistance, Neoplasm; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology*; D017671:Platinum Compounds / Q000627:therapeutic use; D011237:Predictive Value of Tests
chemical_list: D000970:Antineoplastic Agents; D017671:Platinum Compounds