Construction of a microsatellite linkage map with two sequenced rice varieties.
Based on the successful development of new microsatellite markers from the data of two whole-sequenced rice varieties, japonica variety Nipponbare and indica variety 9311, an F2 population of 90 lines, which was derived from a single cross between Nipponbare and 9311, was applied to construct a genetic linkage framework map. The map covered 2 455.7 cM of total genomic length, and consisted of 152 simple sequence repeats (SSRs) loci including 46 pairs of new SSR primers developed by our research institute. The average genetic distance between two markers was 16.16 cM. In addition, markers RM345 and RM494, which have not been mapped on the Temnykh's map et al. (2001) were anchored on the sixth chromosome of this map. We compared this research with maps of Temnykh et al.(2001) and LAN et al. (2003) regarding the aspects of type and size of population, type and quantity of markers, and the marker arrangement order on chromosome, etc. Results indicated that the similarity of marker linear alignment was 93.81% between this map and T-map. Finally, the important significance of using sequenced rice varieties to construct linkage map was also discussed.
Zhang
Qi-Jun
QJ
Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, China.
Ye
Shao-Ping
SP
Li
Jie-Qin
JQ
Zhao
Bing
B
Liang
Yong-Shu
YS
Peng
Yong
Y
Li
Ping
P
D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D008040:Genetic Linkage; D018895:Microsatellite Repeats / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D018744:DNA, Plant