[Recombinant non-structural 3A, 3B and 3AB proteins of foot-and-mouth disease virus: use for differentiation of vaccinated and infected cattle].
Recombinant proteins 3A, 3B and 3AB were obtained by expression in Escherichia coli and purified by metal-chelate chromatography. The proteins were used as antigens in indirect ELISA to differentiate vaccinated and infected cattle. While testing 200 sera from cattle 3A-ELISA was more sensitive and specific than 3B- and 3AB-ELISA. Compared with "Chekit FMD-3ABC", 3A-ELISA showed the same level of specificity and higher level of sensitivity.
Iakovleva
A S
AS
Shcherbakov
A V
AV
Kan'shina
A V
AV
Mudrak
N S
NS
Fomina
T A
TA
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002353:Carrier State / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000175:diagnosis / Q000517:prevention & control / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000956:Antigens, Viral; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines