title: Phospholipid fatty acid composition and diamine oxidase activity of intestinal mucosa from rats treated with irinotecan hydrochloride (CPT-11) under vegetable oil-enriched diets: comparison between perilla oil and corn oil.
abstract: BACKGROUND
  Irinotecan hydrochloride (CPT-11), a topoisomerase I inhibitor highly effective for various cancers, has its dosage limited by diffuse mucosal damage with increased prostaglandin (PG) E(2). However, an analysis of intestinal phospholipid fatty acid composition after CPT-11 treatment has not been reported. This study aimed to evaluate intestinal phospholipid fatty acid composition in relation to intestinal mucosal integrity and plasma and mucosal PGE(2) levels after CPT-11 treatment. The effect of dietary vegetable oil supplementation, perilla oil vs corn oil, was also evaluated.
  
  
  METHODS
  Intestinal phospholipid fatty acid composition, PGE(2) level, mucosal diamine oxidase (DAO) activity, diarrhea, and blood tests were evaluated in rats injected with CPT-11 under a conventional diet. The same parameters were compared among 3 different dietary vegetable oil supplementations: perilla oil, corn oil, and a 1:3, respectively, mixture with a semisynthetic diet during 14 days.
  
  
  RESULTS
  CPT-11 treatment caused severe diarrhea, and intestinal mucosal fatty acid composition changed with increased PGE(2) level and decreased DAO activity. Decreases in eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and EPA/arachidonic acid (AA) ratio in colonic mucosa were observed. Perilla oil increased omega-3 polyunsaturated fatty acids, alpha-linolenic acid, EPA, and EPA/AA ratio and decreased plasma PGE(2). But the amounts used were not enough to attenuate intestinal damage from CPT-11 treatment.
  
  
  CONCLUSIONS
  CPT-11 induced changes of intestinal mucosal fatty acid composition with increased PGE(2) level and decreased intestinal integrity; perilla oil shows the possibility of being able to attenuate those changes.
authors.affiliation: Division of Surgical Metabolism, Faculty of Health Science, Kobe University School of Medicine, 7-10-2 Tomogaoka, Suma-ku, Kobe 654-0142, Japan. musa@ams.kobe-u.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Usami
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ohata
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kishimoto
authors.forename: Keiko
authors.initials: K
authors.lastname: Ohmae
authors.forename: Michiko
authors.initials: M
authors.lastname: Aoyama
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyoshi
authors.forename: Yuri
authors.initials: Y
authors.lastname: Fueda
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000009:adverse effects; D016718:Arachidonic Acid / Q000032:analysis; D002166:Camptothecin / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives*; D003314:Corn Oil / Q000008:administration & dosage; D004032:Diet; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D015232:Dinoprostone / Q000032:analysis / Q000097:blood; D004281:Docosahexaenoic Acids / Q000032:analysis; D015118:Eicosapentaenoic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D015525:Fatty Acids, Omega-3 / Q000032:analysis; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000201:enzymology; D000077146:Irinotecan; D008297:Male; D010743:Phospholipids / Q000032:analysis*; D010938:Plant Oils / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000032:analysis
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D010743:Phospholipids; D010938:Plant Oils; D017962:alpha-Linolenic Acid; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; C058216:perilla seed oil; D000077146:Irinotecan; D003314:Corn Oil; D015118:Eicosapentaenoic Acid; D006631:Amine Oxidase (Copper-Containing); D015232:Dinoprostone; D002166:Camptothecin