Crystallization and preliminary X-ray crystallographic analysis of the GluR0 ligand-binding core from Nostoc punctiforme.
GluR0 from Nostoc punctiforme (NpGluR0) is a bacterial homologue of the ionotropic glutamate receptor. The ligand-binding core of NpGluR0 was crystallized at 294 K using the hanging-drop vapour-diffusion method. The L-glutamate-complexed crystal belongs to space group C222(1), with unit-cell parameters a = 78.0, b = 145.1, c = 132.1 A. The crystals contain three subunits in the asymmetric unit, with a VM value of 2.49 A3 Da(-1). The diffraction limit of the L-glutamate complex data set was 2.1 A using synchrotron X-ray radiation at beamline BL-4A of the Pohang Accelerator Laboratory (Pohang, Korea).
Lee
Jun Hyuck
JH
Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, South Korea.
Park
Soo Jeong
SJ
Rho
Seong-Hwan
SH
Im
Young Jun
YJ
Kim
Mun-Kyoung
MK
Kang
Gil Bu
GB
Eom
Soo Hyun
SH
D000595:Amino Acid Sequence; D015925:Cryopreservation; D003460:Crystallization; D018360:Crystallography, X-Ray; D004058:Diffusion; D018698:Glutamic Acid / Q000737:chemistry; D008024:Ligands; D008969:Molecular Sequence Data; D046937:Nostoc / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D017470:Receptors, Glutamate / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D017356:Synchrotrons; D013696:Temperature; D014961:X-Ray Diffraction
D008024:Ligands; D017470:Receptors, Glutamate; D018698:Glutamic Acid