title: Crystallization of the C-terminal domain of the addiction antidote CcdA in complex with its toxin CcdB.
abstract: CcdA and CcdB are the antidote and toxin of the ccd addiction module of Escherichia coli plasmid F. The CcdA C-terminal domain (CcdAC36; 36 amino acids) was crystallized in complex with CcdB (dimer of 2 x 101 amino acids) in three different crystal forms, two of which diffract to high resolution. Form II belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 37.6, b = 60.5, c = 83.8 A and diffracts to 1.8 A resolution. Form III belongs to space group P2(1), with unit-cell parameters a = 41.0, b = 37.9, c = 69.6 A, beta = 96.9 degrees, and diffracts to 1.9 A resolution.
authors.affiliation: Department of Molecular and Cellular Interactions, Vlaams Interinuversitair Instituut voor Biotechnologie and Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
authors.forename: Lieven
authors.initials: L
authors.lastname: Buts
authors.forename: Natalie
authors.initials: N
authors.lastname: De Jonge
authors.forename: Remy
authors.initials: R
authors.lastname: Loris
authors.forename: Lode
authors.initials: L
authors.lastname: Wyns
authors.forename: Minh-Hoa
authors.initials: MH
authors.lastname: Dao-Thi
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001427:Bacterial Toxins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D019143:Evolution, Molecular; D008565:Membrane Proteins / Q000737:chemistry*; D015233:Models, Statistical; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D014118:Toxins, Biological / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; C104882:CcdA protein, Bacillus; C068356:CcdB protein, Plasmid F; D008565:Membrane Proteins; D014118:Toxins, Biological