title: [Effects of extract of Ginkgo biloba on intercellular adhesion molecule-1 and mRNA expression in rats with cerebral injury].
abstract: OBJECTIVE
  To investigate the effects of Extract of Ginkgo biloba on ICAM-1 and mRNA expression in rats with cerebral injury, and discuss its protective mechanism.
  
  
  METHODS
  The acute closed brain injury model was set up in rats according Feeney's method. Seventy-two rats were randomly divided into four groups. The levels of ICAM-1 expression were observed using immunohistochemistry and computerized imaging technique and the expression of mRNA were studied using RT-PCR techniques.
  
  
  RESULTS
  ICAM-1 positive cells located in the transitional zone between the necrotic core and normal cortex. ICAM-1 protein and mRNA expression began to show 6 hours after cerebral injury, peaked at 24 hours. As compared with the model group, ICAM-1 and mRNA expression was significantly reduced in the group EGb (P < 0.01).
  
  
  CONCLUSIONS
  EGb can markedly reduce the injury of nerve cell in the transitional zone, alleviate rat cerebral injury, and decrease ICAM-1 and mRNA expression and neutrophils infiltration. The protection may be related with its inhibition on ICAM-1 and mRNA expression.
authors.affiliation: Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041.
authors.forename: Rongjun
authors.initials: R
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: You
authors.forename: Bowen
authors.initials: B
authors.lastname: Cai
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.forename: Jianjian
authors.initials: J
authors.lastname: Tang
authors.forename: Zhan
authors.initials: Z
authors.lastname: Liu
authors.forename: Qingfang
authors.initials: Q
authors.lastname: Jiao
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D001930:Brain Injuries / Q000378:metabolism*; D005260:Female; D020441:Ginkgo biloba / Q000737:chemistry*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018696:Neuroprotective Agents; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1