Development of a dual fluorogenic and chromogenic dipeptidyl peptidase IV substrate.
A new far-red dual fluorogenic and chromogenic substrate, 5-glycylprolylglycylprolyl-9-di-3-sulfonyl-propylaminobenza[a]phenoxazonium perchlorate (GPGP-2SBPO), was developed for dipeptidyl peptidase IV (DPP-IV) sensing. The glycylprolylglycylprolyl tetrapeptide was chosen as the recognition sequence due to its stability under physiological conditions. In contrast, the truncated substrate, GP-2SBPO, containing only a glycylprolyl peptide, is unstable. Proteolysis of GPGP-2SBPO was assayed by monitoring the absorbance and fluorescence signals from the released fluorochrome, 2SBPO, at 625 and 670nm, respectively.
Ho
Nan-Hui
NH
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
Weissleder
Ralph
R
Tung
Ching-Hsuan
CH
D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006868:Hydrolysis; D009842:Oligopeptides / Q000737:chemistry*; D013379:Substrate Specificity
C512305:5-glycylprolylglycylprolyl-9-di-3-sulfonyl-propylaminobenza(a)phenoxazonium perchlorate; D005456:Fluorescent Dyes; D009842:Oligopeptides; D018819:Dipeptidyl Peptidase 4