title: Simultaneous determination of three local anesthetic drugs from the pipecoloxylidide group in human serum by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic (HPLC) method has been developed for the simultaneous analysis of the local anesthetic amide drugs, bupivacaine, mepivacaine and ropivacaine, belonging to the pipecoloxylidide group using a C(18) reversed-phase column (150 x 4.6 mm I.D.) filled with 5-microm particles and attached to a UV detector. The mobile phase was composed of acetonitrile-methanol-30 mM NaH(2)PO(4) (pH 5.6) (100:100:300, v/v/v) and the flow rate was 1ml/min. The absorbance of the eluate was monitored at 210 nm. The retention times of the three compounds were: 4.6 min (mepivacaine), 9.7min (ropivacaine) and 16.4 min (bupivacaine). With this sample preparation method, good and consistent recoveries of the three compounds were obtained: 88-91% for mepivacaine, 87-89% for ropivacaine and 88-91% for bupivacaine. The limit of quantification for three compounds in human serum was 2 ng/ml for mepivacaine, 5 ng/ml for bupivacaine and ropivacaine. This method may be useful in clinical and forensic applications for the determination or identification of the local anesthetic drugs: bupivacaine, mepivacaine or ropivacaine.
authors.affiliation: Institute of Community Medicine, University of Tsukuba, Ibaraki-ken 305-8575, Japan. einosuke@md.tsukuba.ac.jp
authors.forename: Einosuke
authors.initials: E
authors.lastname: Tanaka
authors.forename: Takako
authors.initials: T
authors.lastname: Nakamura
authors.forename: Shinichi
authors.initials: S
authors.lastname: Inomata
authors.forename: Katsuya
authors.initials: K
authors.lastname: Honda
mesh_terms: D000577:Amides / Q000097:blood*; D000779:Anesthetics, Local / Q000097:blood*; D002045:Bupivacaine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008619:Mepivacaine / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D000077212:Ropivacaine; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000577:Amides; D000779:Anesthetics, Local; D000077212:Ropivacaine; D008619:Mepivacaine; D002045:Bupivacaine