title: Heterogeneous aspects of lipid oxidation in dried microencapsulated oils.
abstract: This work was aimed at studying lipid oxidation in dried microencapsulated oils (DMOs) during long-term storage. Samples were prepared by freeze-drying of emulsions containing sodium caseinate and lactose as encapsulating components. Evaluation of lipid oxidation was approached by quantitative analysis of nonvolatile lipid oxidation products and tocopherol. Lipid oxidation products were analyzed by separation of polar compounds by adsorption chromatography followed by HPSEC with refraction index detection for quantitation of oxidized triglyceride monomers, dimers, and oligomers. The analytical method applied enabled the detection of different oxidative patterns between the free and encapsulated oil fractions. The free oil fraction of DMOs showed a typical oxidative pattern for oils in continuous phase, which consisted of a clear induction period, in which hydroperoxides (oxidized triglyceride monomers) accumulated, before oxidation accelerated. The end of the induction period was marked by the total loss of tocopherol and the initiation of polymerization. On the contrary, the encapsulated oil showed a pattern characteristic of a mixture of oils with different oxidation status. Thus, high contents of advanced oxidation compounds (polymerization compounds) were detected when the antioxidant (tocopherol) was still present in high amounts. It is concluded that the encapsulated oil was comprised of oil globules with very different oxidation status. The results obtained in this study gave evidence of heterogeneous aspects of lipid oxidation in a dispersed-lipid food system.
authors.affiliation: Instituto de la Grasa (CSIC), Avenida Padre García Tejero 4, 41012 Sevilla, Spain.
authors.forename: Joaquín
authors.initials: J
authors.lastname: Velasco
authors.forename: Susana
authors.initials: S
authors.lastname: Marmesat
authors.forename: Carmen
authors.initials: C
authors.lastname: Dobarganes
authors.forename: Gloria
authors.initials: G
authors.lastname: Márquez-Ruiz
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002214:Capsules; D003890:Desiccation; D005612:Freeze Drying; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D010938:Plant Oils / Q000737:chemistry*; D024505:Tocopherols / Q000032:analysis; D014280:Triglycerides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002214:Capsules; D010938:Plant Oils; D014280:Triglycerides; D024505:Tocopherols