Expression, purification, crystallization and preliminary X-ray analysis of a C-terminal fragment of the Epstein-Barr virus ZEBRA protein.
A C-terminal fragment of the Epstein-Barr virus immediate-early transcription factor ZEBRA has been expressed as a recombinant protein in Escherichia coli and purified to homogeneity. The fragment behaves as a dimer in solution, consistent with the presence of a basic region leucine-zipper (bZIP) domain. Crystals of the fragment in complex with a DNA duplex were grown by the hanging-drop vapour-diffusion technique using polyethylene glycol 4000 and magnesium acetate as crystallization agents. Crystals diffract to better than 2.5 A resolution using synchrotron radiation (lambda = 0.976 A). Crystals belong to space group C2, with unit-cell parameters a = 94.2, b = 26.5, c = 98.1 A, beta = 103.9 degrees.
Morand
Patrice
P
European Molecular Biology Laboratory, Grenoble Outstation, BP 181, 38042 Grenoble Cedex 9, France.
Budayova-Spano
Monika
M
Perrissin
Monique
M
Müller
Christoph W
CW
Petosa
Carlo
C
D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000737:chemistry*; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000302:isolation & purification; D010446:Peptide Fragments / Q000737:chemistry; D020836:Protein Structure, Quaternary; D015534:Trans-Activators / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D014764:Viral Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification
C063676:BZLF1 protein, Herpesvirus 4, Human; D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D010446:Peptide Fragments; D015534:Trans-Activators; D014764:Viral Proteins