Overexpression of PRE1 and its homologous genes activates Gibberellin-dependent responses in Arabidopsis thaliana.
Gibberellins control various aspects of growth and development. Here, we identified a gene, designated paclobutrazol resistance1 (PRE1), by screening Arabidopsis activation-tagged lines. PRE1 encodes a helix-loop-helix protein and belongs to a small gene family. Physiological and genetic analysis indicated that overexpression of PRE1 altered various aspects of gibberellin-dependent responses such as germination, elongation of hypocotyl/petiole, floral induction and fruit development, and suppressed gibberellin-deficient phenotypes of the ga2 mutant. Expression of some gibberellin-responsive genes was also affected by PRE1. Expression of PRE1 was shown to be early gibberellin inducible in the wild-type plants and under control of SPY and GAI, upstream negative regulators of gibberellin signaling. The shortened hypocotyl length phenotype of the gai-1 mutant was suppressed by PRE1 overexpression. Ectopic overexpression of each of the four PRE1-related genes conferred pleiotropic phenotypes similar to PRE1 overexpression, indicative of overlapping functions among the PRE gene family. Our results of gain-of-function studies suggest that PRE genes may have a regulatory role in gibberellin-dependent development in Arabidopsis thaliana.
Lee
Sumin
S
Department of Molecular Biology, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul 143-747, Republic of Korea.
Lee
Seunghee
S
Yang
Ki-Young
KY
Kim
Young-Mi
YM
Park
So-Yeon
SY
Kim
Soo Young
SY
Soh
Moon-Soo
MS
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology*; D017343:Genes, Plant / Q000235:genetics / Q000502:physiology*; D018525:Germination / Q000235:genetics / Q000502:physiology; D005875:Gibberellins / Q000235:genetics / Q000502:physiology*; D018257:Helix-Loop-Helix Motifs / Q000235:genetics / Q000502:physiology; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D030821:Plants, Genetically Modified; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
D029681:Arabidopsis Proteins; D018744:DNA, Plant; D005875:Gibberellins; C513126:PRE1 protein, Arabidopsis; D014157:Transcription Factors