Structure of Citrobacter freundii L-methionine gamma-lyase.
L-Methionine gamma-lyase (MGL) is a pyridoxal 5'-phosphate (PLP) dependent enzyme that catalyzes gamma-elimination of L-methionine. The crystal structure of MGL from Citrobacter freundii has been determined at 1.9 A resolution. The spatial fold of the protein is similar to those of MGLs from Pseudomonas putida and Trichomonas vaginalis. The comparison of these structures revealed that there are differences in PLP-binding residues and positioning of the surrounding flexible loops.
Mamaeva
D V
DV
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 119991 Moscow, Russia.
Morozova
E A
EA
Nikulin
A D
AD
Revtovich
S V
SV
Nikonov
S V
SV
Garber
M B
MB
Demidkina
T V
TV
D001426:Bacterial Proteins / Q000737:chemistry; D001665:Binding Sites; D013437:Carbon-Sulfur Lyases / Q000737:chemistry*; D016971:Citrobacter freundii / Q000201:enzymology*; D018360:Crystallography, X-Ray; D015394:Molecular Structure; D017433:Protein Structure, Secondary
D001426:Bacterial Proteins; D013437:Carbon-Sulfur Lyases; C021558:L-methionine gamma-lyase