Detection of Newcastle disease virus using nucleic acid sequence-based amplification.
Newcastle disease (ND) is a contagious and widespread avian disease affecting most species of birds. ND virus (NDV) is the only member of the avian paramyxovirus serotype 1 (APMV1) causing ND outbreak in bird flocks. The technique of nucleic acid sequence-based amplification (NASBA) is a potential method to rapidly and reliably detect NDV isolates. Here, we describe an effective and unprecedented method for detecting NDV strains of all pathotypes. A conserved region of the fusion protein gene was used for designing oligonucleotides specific to all NDV pathotypes. The dynamic range of this NDV NASBA detection method is comparable to virus culture and therefore the NDV NASBA method is a potential alternative for NDV screening and surveillance.
Cui
Shang-Jin
SJ
National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, No. 427 Maduan Street, Harbin 150001, China.
Fung
Yin-Wan Wendy
YW
Lau
Lok Ting
LT
Liu
Wen-Bin
WB
Wang
Yun-Feng
YF
Tong
Guang-Zhi
GZ
Chen
Jianguo
J
Yu
Albert Cheung Hoi
AC
D000818:Animals; D001717:Birds; D002645:Chickens; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics*; D005784:Gene Amplification; D009521:Newcastle Disease / Q000175:diagnosis*; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010641:Phenotype; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*
D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral