In vitro antioxidant activity and antigenotoxicity of 5-n-alkylresorcinols.
Incubation with 5-n-alkylresorcinols (chain lengths C15:0, C17:0, C19:0, C21:0, and C23:0) increased the self-protection capacity of HT29 human colon cancer cells against DNA damage induced by hydrogen peroxide and genotoxic fecal water samples using comet assay (single-cell gel electrophoresis assay). The alkylresorcinols did not exert potent antioxidant activity in the FRAP (ferric reduction ability of plasma) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical assays. However they were able to significantly inhibit copper-mediated oxidation of human LDL (low-density lipoprotein) in vitro, and pentadecylresorcinol at 25 micromol/L increased lag time by 65 min. The results show that alkylresorcinols have antigenotoxic and antioxidant activity under in vitro conditions.
Parikka
Kirsti
K
Laboratory of Organic Chemistry, Department of Chemistry, University of Helsinki, P.O. Box 55, FIN-00014 Helsinki, Finland. kirsti.parikka@helsinki.fi
Rowland
Ian R
IR
Welch
Robert W
RW
Wähälä
Kristiina
K
D000478:Alkylation; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D003300:Copper / Q000737:chemistry; D004249:DNA Damage / Q000187:drug effects; D005243:Feces / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D019073:HT29 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000737:chemistry; D009153:Mutagens; D010084:Oxidation-Reduction; D010851:Picrates; D012118:Resorcinols / Q000737:chemistry / Q000494:pharmacology*
D016587:Antimutagenic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005290:Ferric Compounds; D008077:Lipoproteins, LDL; D009153:Mutagens; D010851:Picrates; D012118:Resorcinols; D003300:Copper; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl