title: Differential expression of two class III chitinases in two types of roots of Quercus robur during pre-mycorrhizal interactions with Piloderma croceum.
abstract: Expression of two plant chitinase genes, representing members of class III chitinases, was studied in Quercus robur roots during interactions in a pre-mycorrhizal stage with the ectomycorrhizal fungus Piloderma croceum. Chitinase gene expression was compared in lateral roots destined to form ectomycorrhiza, and in principal roots that are not directly involved in mycorrhizal interactions. The transcript level of the first chitinase (QrchitIII-1) was upregulated in lateral roots, whereas no significant differential expression was observed in principal roots. The second chitinase (QrchitIII-2) was regulated neither in lateral nor in principal roots in presence of the fungus. Because P. croceum did not induce significant chitinase responses in principal roots, the enhanced expression of QrchitIII-1 in lateral roots after inoculation may be related to some steps in symbiosis ontogenesis.
authors.affiliation: Department of Terrestrial Ecology, Institute of Botany I, University of Leipzig, Johannisallee 21-23, 04103, Leipzig, Germany.|"Tree-Microbe Interactions Unit", Institut National de la Recherche Agronomique, Champenoux, 54280, France.|Institute of General Botany and Plant Physiology, University of Jena, Dornburger Straβe 159, 07743, Jena, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Frettinger
authors.affiliation: Department of Terrestrial Ecology, Institute of Botany I, University of Leipzig, Johannisallee 21-23, 04103, Leipzig, Germany.|Institute of Ecology, University of Jena, Dornburger Straβe 159, 07743, Jena, Germany.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Herrmann
authors.affiliation: "Tree-Microbe Interactions Unit", Institut National de la Recherche Agronomique, Champenoux, 54280, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lapeyrie
authors.affiliation: Institute of General Botany and Plant Physiology, University of Jena, Dornburger Straβe 159, 07743, Jena, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Oelmüller
authors.affiliation: Department of Terrestrial Ecology, Institute of Botany I, University of Leipzig, Johannisallee 21-23, 04103, Leipzig, Germany. francois.buscot@ufz.de.|Center for Environmental Research (UFZ), Department for Soil Ecology, Theodor-Lieser-Straβe 4, 06120, Halle, Germany. francois.buscot@ufz.de.
authors.forename: François
authors.initials: F
authors.lastname: Buscot
mesh_terms: D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D038821:Mycorrhizae / Q000502:physiology*; D010940:Plant Proteins; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000382:microbiology; D029963:Quercus / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D013559:Symbiosis
chemical_list: D010940:Plant Proteins; D002688:Chitinases; C110892:chitinase, class III