title: [Construction and expression of fusion gene encoding Hyper-IL-6 in mammalian cells].
abstract: OBJECTIVE
  To construct and express fusion gene encoding Hyper-IL-6 in mammalian cells.
  
  
  METHODS
  Using geneSOEing method, human sIL-6R cDNA was fused with IL-6 cDNA by a linker rich in glycine through PCR technique and cloned into pIRES(2)-EGFP. The constructed plasmid was transfected into mammalian cells. The expression of fusion gene encoding Hyper-IL-6 was detected by GFP and RT-PCR and immunocytochemistry.
  
  
  RESULTS
  The recombinant plasmid pIRES-HIL-6 was successfully constructed and expressed in mammalian cells.
  
  
  CONCLUSIONS
  The success in construction and expression of human Hyper-IL-6 makes it possible to further study its biological functions.
authors.affiliation: Viral Institute for Hepatitis, Key Laboratory of Molecular Biology on Infectious Diseases of Ministry of Education, Chongqing University of Medical Sciences, Chongqing 400010, China.
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Qie
authors.forename: Bo
authors.initials: B
authors.lastname: Qin
authors.forename: Ai-long
authors.initials: AL
authors.lastname: Huang
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Guo-min
authors.initials: GM
authors.lastname: Chen
mesh_terms: D000818:Animals; D018076:DNA, Complementary; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D018076:DNA, Complementary; D004274:DNA, Recombinant; D015850:Interleukin-6; D011993:Recombinant Fusion Proteins