title: Hepatitis C virus-related internal ribosome entry sites are found in multiple genera of the family Picornaviridae.
abstract: The internal ribosome entry site (IRES) elements from porcine enterovirus 8 and simian virus 2, two members of a proposed new genus within the family Picornaviridae, were characterized. These IRES elements, in common with the porcine teschovirus 1 IRES, were found to be related functionally and structurally to the IRES element from Hepatitis C virus, a member of the family Flaviviridae. Partial secondary structure predictions were derived and functional assays demonstrated that these IRES elements continued to be active when eIF4G was cleaved and when the activity of eIF4A was blocked.
authors.affiliation: BBSRC, Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, UK.
authors.forename: Louisa S
authors.initials: LS
authors.lastname: Chard
authors.affiliation: Department of Biochemistry, McIntyre Medical Sciences Building, Montreal, QC H3G 1Y6, Canada.
authors.forename: Marie-Eve
authors.initials: ME
authors.lastname: Bordeleau
authors.affiliation: McGill Cancer Center, McIntyre Medical Sciences Building, Montreal, QC H3G 1Y6, Canada.|Department of Biochemistry, McIntyre Medical Sciences Building, Montreal, QC H3G 1Y6, Canada.
authors.forename: Jerry
authors.initials: J
authors.lastname: Pelletier
authors.affiliation: Department of Chemistry, Biology and Marine Sciences, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Tanaka
authors.affiliation: BBSRC, Institute for Animal Health, Pirbright, Woking, Surrey GU24 0NF, UK.
authors.forename: Graham J
authors.initials: GJ
authors.lastname: Belsham
mesh_terms: D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D018387:Codon, Initiator; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004771:Enteroviruses, Porcine / Q000235:genetics* / Q000472:pathogenicity; D016174:Hepacivirus / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D008969:Molecular Sequence Data; D010849:Picornaviridae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism*; D013552:Swine / Q000821:virology
chemical_list: D020121:5' Untranslated Regions; D018387:Codon, Initiator; D018076:DNA, Complementary; D012367:RNA, Viral