title: Measurement of the free concentration of octylphenol in biological samples with negligible depletion-solid phase microextraction (nd-SPME): analysis of matrix effects.
abstract: A negligible depletion-solid phase microextraction (nd-SPME) method is presented to measure free concentrations of octylphenol in biological samples. Potential confounding factors, such as matrix effects, are studied as well. Fouling of the fibre appears to occur, but it does not seem to reduce or enhance the measured uptake of octylphenol. In the setup applied here, without any agitation, it has also been found that there is a large effect of protein presence on the kinetics of octylphenol uptake. In addition, an apparent affinity constant of octylphenol for bovine serum albumin was determined.
authors.affiliation: Institute of Risk Assessment Sciences, IRAS, Utrecht University, P.O. Box 80176, 3508 TD Utrecht, The Netherlands. Minne.Heringa@kiwa.nl
authors.forename: Minne B
authors.initials: MB
authors.lastname: Heringa
authors.forename: Chris
authors.initials: C
authors.lastname: Hogevonder
authors.forename: Frans
authors.initials: F
authors.lastname: Busser
authors.forename: Joop L M
authors.initials: JL
authors.lastname: Hermens
mesh_terms: D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D010636:Phenols / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D003470:Culture Media; D010636:Phenols; C474055:octylphenol; D012710:Serum Albumin, Bovine