Activation of mitogen activated protein kinase (MAPK) during D-galactosamine intoxication in the rat liver.
A significant increase in plasma glutamate-oxaloacetate transaminase and glutamate-pyruvate transaminase was observed 6 h after intraperitoneal administration of D-galactosamine (D-Galn). Three hours after administration of D-Galn, the vitamin C concentration in the liver decreased significantly compared to that in a control group and thereafter the hepatic vitamin C concentration remained at a significantly lower level. Phosphorylated JNK (c-Jun NH2-terminal kinase) and phosphorylated ERK (extracellular signal-regulated kinase) started increasing 3 h after D-Galn treatment and remained at a high level for 6-12 h after the treatment, while phosphorylated p38 MAPK increased significantly 6 h after D-Galn administration. These results indicated that oxidative stress and the activation of JNK and ERK took place almost simultaneously, followed by the activation of p38 MAPK.
Nishioka
Hitomi
H
Department of Food Science and Nutrition, Nara Women's University, Nara 630-8506, Japan.
Kishioka
Terumi
T
Iida
Chinatsu
C
Fujii
Kozue
K
Ichi
Ikuyo
I
Kojo
Shosuke
S
D019883:Alkyl and Aryl Transferases / Q000378:metabolism; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D005688:Galactosamine / Q000378:metabolism* / Q000506:poisoning*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D051381:Rats; D017855:Transferases (Other Substituted Phosphate Groups) / Q000378:metabolism
D005688:Galactosamine; D019883:Alkyl and Aryl Transferases; C112459:geranylpyrophosphate olivetolate geranyltransferase; D020928:Mitogen-Activated Protein Kinases; D017855:Transferases (Other Substituted Phosphate Groups); C106504:UDP-GlcNAc-undecaprenyl phosphate N-acetylglucosaminyl 1-phosphate transferase