A fluorescent nanosensor for apoptotic cells.
A biocompatible surface-functionalized nanoparticle was designed to sense phosphatidylserine exposed on apoptotic cells. We conjugated synthetic artificial phosphatidylserine binding ligands in a multivalent fashion onto magnetofluorescent nanoparticles. Our results show that (1) the synthetic nanoparticles bind to apoptotic cells, (2) there is excellent correlation with annexin V staining by microscopy, and (3) FACS analysis with nanoparticles allows the measurement of therapeutic apoptosis induction. The described nanomaterials should be useful for a variety of biomedical applications including in vivo imaging of apoptosis.
Quinti
Luisa
L
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, 02129, USA.
Weissleder
Ralph
R
Tung
Ching-Hsuan
CH
D017209:Apoptosis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D005434:Flow Cytometry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis*; D006801:Humans; D019169:Jurkat Cells; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*
D005456:Fluorescent Dyes