title: Intraprostatic lymphocyte profiles in aged wistar rats with estradiol induced prostate inflammation.
abstract: OBJECTIVE
  We report LS phenotypes in aged Wistar rats (Charles Rivers, Wilmington, Massachusetts) with EPI.
  
  
  METHODS
  EPI was induced in 36 to 40-week-old male rats by castration with subcutaneous injections of 17beta-estradiol (0.25 mg/kg daily) plus dihydrotestosterone propionate (2.5 mg/kg daily) in sesame oil for 30 days. Controls were sham castrated, aged rats that were injected with sesame oil. Prostate, spleen and blood LSs in aged and young (10 to 12-week-old) rats were identified by flow cytometry in a cluster of differentiation system.
  
  
  RESULTS
  All prostates in 6, 17beta-estradiol plus dihydrotestosterone propionate treated rats and in 3 of 7 controls (43%) showed inflammation foci. All studied LSs in Aged-SPIs and Aged-EPIs were similar. Blood LSs in Aged-SPIs, Aged-EPIs, Aged-NPIs and Youngs showed no differences. Levels of lymphocytes bearing the natural killer marker were decreased, and levels of total T and CD4(+) T cells were increased in prostates with age. Intraprostatic and splenic levels of CD4(+) natural killer T cells were down-regulated significantly in Aged-SPIs and Aged-EPIs compared to those in Aged-NPIs and Youngs. Levels of CD45RC(+)CD4(+)alphabetaTCR(+) T cells were decreased 2-fold in the spleen and up-regulated 2-fold in the prostate of Aged-SPIs and Aged-EPIs compared to those in Aged-NPIs and Youngs.
  
  
  CONCLUSIONS
  Similar LS features in Aged-SPIs and Aged-EPIs may indicate that the EPI model is appropriate for studies of the immune aspects of prostate inflammation. Imbalance between suppressive CD4(+) natural killer T cells and autoreactive CD45RC(+)CD4(+)alphabetaTCR(+) T cells in Aged-SPIs and Aged-EPIs may suggest their role in prostate inflammation in this model.
authors.affiliation: Jim and Eilleen Dicke Research Laboratory, Department of Urology, School of Medicine, Case Western Reserve University-University Hospitals of Cleveland, Cleveland, Ohio, USA. yvv@case.edu
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Vykhovanets
authors.forename: Martin I
authors.initials: MI
authors.lastname: Resnick
authors.forename: Susan Ruth
authors.initials: SR
authors.lastname: Marengo
mesh_terms: D000367:Age Factors; D000818:Animals; D004958:Estradiol / Q000008:administration & dosage; D018655:Lymphocyte Count; D008214:Lymphocytes; D008297:Male; D011472:Prostatitis / Q000139:chemically induced / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004958:Estradiol