title: Biocompatibility of cluster-assembled nanostructured TiO2 with primary and cancer cells.
abstract: We have characterized the biocompatibility of nanostructured TiO2 films produced by the deposition of a supersonic beam of TiOx clusters. Physical analysis shows that these films possess, at the nanoscale, a granularity and porosity mimicking those of typical extracellular matrix structures and adsorption properties that could allow surface functionalization with different macromolecules such as DNA, proteins, and peptides. To explore the biocompatibility of this novel nanostructured surface, different cancer and primary cells were analyzed in terms of morphological appearance (by bright field microscopy and immunofluorescence) and growth properties, with the aim to evaluate cluster-assembled TiO2 films as substrates for cell-based and tissue-based applications. Our results strongly suggest that this new biomaterial supports normal growth and adhesion of primary and cancer cells with no need for coating with ECM proteins; we thus propose this new material as an optimal substrate for different applications in cell-based assays, biosensors or microfabricated medical devices.
authors.affiliation: European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy. roberta.carbone@ifom-ieo-campus.it
authors.forename: Roberta
authors.initials: R
authors.lastname: Carbone
authors.forename: Ida
authors.initials: I
authors.lastname: Marangi
authors.forename: Andrea
authors.initials: A
authors.lastname: Zanardi
authors.forename: Luca
authors.initials: L
authors.lastname: Giorgetti
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Chierici
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Berlanda
authors.forename: Alessandro
authors.initials: A
authors.lastname: Podestà
authors.forename: Francesca
authors.initials: F
authors.lastname: Fiorentini
authors.forename: Gero
authors.initials: G
authors.lastname: Bongiorno
authors.forename: Paolo
authors.initials: P
authors.lastname: Piseri
authors.forename: Pier Giuseppe
authors.initials: PG
authors.lastname: Pelicci
authors.forename: Paolo
authors.initials: P
authors.lastname: Milani
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D003599:Cytoskeleton / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008027:Light; D008422:Materials Testing; D008544:Melanocytes / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D043942:Nanotubes; D009369:Neoplasms / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D012542:Scattering, Radiation; D013499:Surface Properties; D013997:Time Factors; D023822:Tissue Engineering; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D010455:Peptides; C009495:titanium dioxide; D004247:DNA; D014025:Titanium; D001973:Bromodeoxyuridine