Dictyostelium discoideum chemotaxis: threshold for directed motion.
The chemotactic response of Dictyostelium discoideum cells to stationary, linear gradients of cyclic adenosine 3',5'-monophosphate (cAMP) was studied using microfluidic devices. In shallow gradients of less than 10(-3) nM/microm, the cells showed no directional response and exhibited a constant basal motility. In steeper gradients, cells moved up the gradient on average. The chemotactic speed and the motility increased with increasing steepness up to a plateau at around 10(-1) nM/microm. In very steep gradients, above 10 nM/microm, the cells lost directionality and the motility returned to the sub-threshold level. In the regime of optimal response the difference in receptor occupancy at the front and back of the cell is estimated to be only about 100 molecules.
Song
Loling
L
Laboratory of Atomic and Solid State Physics, Department of Physics, Cornell University, Ithaca, NY 14853, USA.
Nadkarni
Sharvari M
SM
Bödeker
Hendrik U
HU
Beta
Carsten
C
Bae
Albert
A
Franck
Carl
C
Rappel
Wouter-Jan
WJ
Loomis
William F
WF
Bodenschatz
Eberhard
E
D000818:Animals; D002633:Chemotaxis / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism; D004023:Dictyostelium / Q000166:cytology / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods
D000242:Cyclic AMP