Establishment and characterization of three immortal bovine muscular epithelial cell lines.
We have established three immortal bovine muscular epithelial (BME) cell lines, one spontaneously immortalized (BMES), the second SV40LT-mediated (BMEV) and the third hTERT-mediated (BMET). The morphology of the three immortal cell lines was similar to that of early passage primary BME cells. Each of the immortal cell lines made cytokeratin, a typical epithelial marker. BMET grew faster than the other immortal lines and the BME cells, in 10% FBS-DMEM medium, whereas neither the primary cells nor the three immortal cell lines grew in 0.5% FBS-DMEM. The primary BME cells and the immortal cell lines, with the exception of BMES, made increasing amounts of p53 protein when treated with doxorubicin, a DNA damaging agent. On the other hand, almost half of the cells in populations of the three immortal cell lines may lack p16(INK4a) regulatory function, compared to primary BME cells that were growth arrested by enforced expression of p16(INK4a). In soft-agar assays, the primary cells and immortal cell lines proved to be less transformed in phenotype than HeLa cells. The three immortal epithelial-type cell lines reported here are the first cell lines established from muscle tissue of bovine or other species.
Jin
Xun
X
The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University, Seoul 136-701, Korea.
Lee
Joong-Seob
JS
Kwak
Sungwook
S
Lee
Soo-Yeon
SY
Jung
Ji-Eun
JE
Kim
Tae-Kyung
TK
Xu
Chenxiong
C
Hong
Zhongshan
Z
Li
Zhehu
Z
Kim
Sun-Myung
SM
Pian
Xumin
X
Lee
Dong-Hee
DH
Yoon
Jong-Taek
JT
You
Seungkwon
S
Choi
Yun-Jaie
YJ
Kim
Huunggee
H
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002460:Cell Line; D002461:Cell Line, Transformed; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D007700:Kinetics; D009132:Muscles / Q000166:cytology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D019941:Cyclin-Dependent Kinase Inhibitor p16; D016159:Tumor Suppressor Protein p53