title: Crystallization and preliminary X-ray diffraction studies of the pneumococcal teichoic acid phosphorylcholine esterase Pce.
abstract: The pneumococcal phosphorylcholine esterase (Pce or CbpE) is a modular protein that hydrolyses the phosphorylcholine residues present in the teichoic and lipoteichoic acids of the pneumococcal cell wall. Pce has been crystallized using the hanging-drop vapour-diffusion method at 291 K. Diffraction-quality monoclinic crystals belong to space group C2, with unit-cell parameters a = 169.82, b = 57.26, c = 67.44 A, beta = 112.60 degrees. A 2.7 A resolution SAD data set from a non-isomorphous Gd-HPDO3A Pce derivative was collected at the gadolinium L(III) absorption edge using synchrotron radiation.
authors.affiliation: Departamento de Química-Física de Macromoléculas Biológicas, Instituto Química-Física Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Lagartera
authors.forename: Ana
authors.initials: A
authors.lastname: González
authors.forename: Meike
authors.initials: M
authors.lastname: Stelter
authors.forename: Pedro
authors.initials: P
authors.lastname: García
authors.forename: Richard
authors.initials: R
authors.lastname: Kahn
authors.forename: Margarita
authors.initials: M
authors.lastname: Menéndez
authors.forename: Juan A
authors.initials: JA
authors.lastname: Hermoso
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D015183:Restriction Mapping; D017384:Sequence Deletion; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013296:Streptococcus pneumoniae / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D010446:Peptide Fragments; D011994:Recombinant Proteins; D002265:Carboxylic Ester Hydrolases; C022718:teichoic acid phosphorylcholine esterase