Tobacco mosaic virus (TMV) and potato virus X (PVX) coat proteins confer heterologous interference to PVX and TMV infection, respectively.
Replication of Potato virus X (PVX) was reduced in transgenic protoplasts that accumulated wild-type coat protein (CPWT) of Tobacco mosaic virus (TMV) or a mutant CP, CP(T42W), that produced highly ordered states of aggregation, including pseudovirions. This reaction is referred to as heterologous CP-mediated resistance. However, protoplasts expressing a CP mutant that abolished aggregation and did not produce pseudovirions, CPT28W, did not reduce PVX replication. Similarly, in transgenic tobacco plants producing TMV CPWT or CP(T42W), there was a delay in local cell-to-cell spread of PVX infection that was not observed in CP(T28W) plants or in non-transgenic plants. The results suggest that the quaternary structure of the TMV CP regulates the mechanism(s) of heterologous CP-mediated resistance. Similarly, transgenic protoplasts that produced PVX CP conferred transient protection against infection by TMV RNA. Transgenic plants that accumulated PVX CP reduced the cell-to-cell spread of infection and resulted in a delay in systemic infection following inoculation with TMV or TMV RNA. Heterologous CP-mediated resistance was characterized by a brief delay in systemic infection, whilst homologous CP-mediated resistance conferred reduced or no systemic infection.
Bazzini
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AA
Instituto de Biotecnología, INTA Castelar, Las Cabañas y Los Reseros, B1712WAA Buenos Aires, Argentina.|Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA.
Asurmendi
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S
Instituto de Biotecnología, INTA Castelar, Las Cabañas y Los Reseros, B1712WAA Buenos Aires, Argentina.|Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA.
Hopp
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HE
Instituto de Biotecnología, INTA Castelar, Las Cabañas y Los Reseros, B1712WAA Buenos Aires, Argentina.
Beachy
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RN
Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, USA.
D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified; D017863:Potexvirus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D014026:Nicotiana / Q000235:genetics / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D014779:Virus Replication
D036022:Capsid Proteins