[Production of miniantibodies to Agrobacterium tumefaciens VirE2 virulence protein by the method of phage display].
The scFv miniantibodies to the recombinant protein VirE2 from Agrobacterium tumefaciens were obtained by the method of phage display. The miniantibodies were purified and tested using timmunodot method for binding to a recombinant protein from Escherichia coli and to the native protein VirE2 from A. tumefaciens. The functional activity of the miniantibodies was comparable to the activity of mouse polyclonal antibodies against the VirE2 protein.
Velikov
V A
VA
Ermoshina
O S
OS
Volokhina
I V
IV
Chumakov
M I
MI
D016960:Agrobacterium tumefaciens / Q000737:chemistry / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000276:immunology; D007473:Ion Channels / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D019151:Peptide Library; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D007135:Immunoglobulin Variable Region; D007473:Ion Channels; D019151:Peptide Library; D011994:Recombinant Proteins; C051583:virE2 protein, Agrobacterium