title: Hybrid dynamic coating with n-dodecyl beta-D-maltoside and methyl cellulose for high-performance carbohydrate analysis on poly(methyl methacrylate) chips.
abstract: Hybrid dynamic coating using n-dodecyl beta-d-maltoside (DDM) and methyl cellulose (MC) has been developed for suppression of analyte adsorption and electroosmotic flow (EOF) in a poly(methyl methacrylate) (PMMA) channel. The adsorption of APTS-labeled sugars in a PMMA channel was obviously suppressed with DDM dynamic coating; however, EOF was reduced only by a factor of approximately 25%, resulting in irreproducible separations. In contrast, both analyte adsorption and EOF in a PMMA channel were efficiently minimized with MC coating; however, concentrated MC above 0.3% was required to achieve high-performance separations, which greatly increased viscosity of the solution and caused difficulties during buffer loading and rinsing. In addition, n-dodecyltrimethylammonium chloride did not show observable effects on reducing analyte adsorption, although it has the same hydrophobic alkyl chain as DDM. These results strongly indicated that the polysaccharide moiety of surface modifiers has a specific affinity to surface charges and is crucial to achieving efficient and stable dynamic coating on the PMMA surface. Hybrid dynamic coating with 0.25% DDM and 0.03% MC was found to minimize both analyte adsorption and EOF in a PMMA channel to a negligible level, while still keeping a low viscosity of the solution. High-speed and high-throughput profiling of the N-linked glycans derived from alpha1-acid glycoprotein, fetuin, and ribonuclease B was demonstrated in both single-channel and 10-channel PMMA chips using DDM-MC hybrid coating. We propose that DDM-MC hybrid coating might be a general method for suppressing analyte adsorption and EOF in polymer MCE devices. The current MCE-based method might be a promising alternative for high-throughput screening of carbohydrate alterations in glycoproteins.
authors.affiliation: Nano-bioanalysis Team, Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho 2217-14, Takamatsu 761-0395, Japan. dangfqn@hotmail.com
authors.forename: Fuquan
authors.initials: F
authors.lastname: Dang
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kakehi
authors.forename: Jingjun
authors.initials: J
authors.lastname: Cheng
authors.forename: Osamu
authors.initials: O
authors.lastname: Tabata
authors.forename: Masaya
authors.initials: M
authors.lastname: Kurokawa
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakajima
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D000327:Adsorption; D002241:Carbohydrates / Q000032:analysis*; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005960:Glucosides; D006023:Glycoproteins / Q000737:chemistry; D008747:Methylcellulose; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000592:standards; D019904:Polymethyl Methacrylate; D011134:Polysaccharides / Q000032:analysis
chemical_list: D002241:Carbohydrates; D005960:Glucosides; D006023:Glycoproteins; D011134:Polysaccharides; C040358:dodecyl maltoside; D008747:Methylcellulose; D019904:Polymethyl Methacrylate