title: Expression, purification, crystallization and preliminary X-ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri.
abstract: Xanthomonas axonopodis pv. citri YaeQ (XAC2396) is a member of a family of bacterial proteins conserved in several Gram-negative pathogens. Here, the cloning, expression, purification and crystallization of the 182-residue (20.6 kDa) YaeQ protein are described. Recombinant YaeQ containing selenomethionine was crystallized in space group P2(1) and crystals diffracted to 1.9 A resolution at a synchrotron source. The unit-cell parameters are a = 39.75, b = 91.88, c = 48.03 A, beta = 108.37 degrees. The calculated Matthews coefficient suggests the presence of two YaeQ molecules in the asymmetric unit. Initial experimental phases were calculated by the multiple-wavelength anomalous dispersion technique and an interpretable electron-density map was obtained.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CEP 05508-000, São Paulo, SP, Brazil.
authors.forename: Cristiane R
authors.initials: CR
authors.lastname: Guzzo
authors.forename: Ronaldo A P
authors.initials: RA
authors.lastname: Nagem
authors.forename: Leonor M P
authors.initials: LM
authors.lastname: Galvão-Botton
authors.forename: Beatriz G
authors.initials: BG
authors.lastname: Guimarães
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Medrano
authors.forename: João A R G
authors.initials: JA
authors.lastname: Barbosa
authors.forename: Chuck S
authors.initials: CS
authors.lastname: Farah
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012645:Selenomethionine / Q000737:chemistry / Q000378:metabolism; D014974:Xanthomonas / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D012645:Selenomethionine