The role of reactive oxygen species in the herbicide acetochlor-induced DNA damage on Bufo raddei tadpole liver.
After exposure of Bufo raddei tadpoles to acetochlor (ACETO) for 14 days, malondialdehyde (MDA) and DNA-single strand break (DNA-SSB) in livers were analyzed. An enhanced accumulation of MDA suggests that ACETO causes oxidative stress, and the significant increase in the level of DNA-SSB indicates that ACETO induces DNA damage in a dose-dependent manner as well. On the basis of the fact that oxidative stress is caused by excessive production of reactive oxygen species (ROS), and the present results, we speculate that ACETO-induced DNA damage may be a consequence of the generation of ROS. To evaluate this hypothesis, tadpoles were treated with ROS scavenger, N-acetyl-L-cysteine (NAC) or melatonin (MEL), prior to ACETO exposure. The decrease of DNA-SSB level and the increase of total antioxidant capability (TAC) show that ACETO-caused DNA damage can be attenuated by NAC and MEL. In addition, a negative correlation was observed between the extent of DNA damage and the level of TAC in tadpole liver. In conclusion, the results suggest that ACETO-induced DNA damage is mediated by ROS.
Liu
Yang
Y
School of Life Sciences, Lanzhou University, Lanzhou 730000, Gansu, China.
Zhang
Yingmei
Y
Liu
Jianghai
J
Huang
Dejun
D
D000111:Acetylcysteine / Q000633:toxicity; D000818:Animals; D002025:Bufonidae / Q000235:genetics / Q000502:physiology*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology; D006540:Herbicides / Q000633:toxicity*; D007814:Larva / Q000187:drug effects / Q000235:genetics; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008550:Melatonin / Q000633:toxicity; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000032:analysis / Q000633:toxicity*; D013223:Statistics as Topic; D014052:Toluidines / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
D016166:Free Radical Scavengers; D006540:Herbicides; D017382:Reactive Oxygen Species; D014052:Toluidines; D014874:Water Pollutants, Chemical; C043377:acetochlor; D004247:DNA; D008550:Melatonin; D000111:Acetylcysteine