Sterilizing Bacillus pumilus spores using supercritical carbon dioxide.
Supercritical carbon dioxide (SC CO(2)) has been evaluated as a new sterilization technology. Results are presented on killing of B. pumilus spores using SC CO(2) containing trace levels of additives. Complete killing was achieved with 200 part per million (ppm) hydrogen peroxide in SC CO(2) at 60 degrees C, 27.5 MPa. Addition of water to SC CO(2) resulted in greater than three-log killing, but this is insufficient to claim sterilization. Neither ethanol nor isopropanol when added to SC CO(2) affected killing.
Zhang
Jian
J
Department of Chemical Engineering, University of South Carolina, Columbia, SC 29208, USA.
Burrows
Sarah
S
Gleason
Courtney
C
Matthews
Michael A
MA
Drews
Michael J
MJ
Laberge
Martine
M
An
Yuehuei H
YH
D019840:2-Propanol; D001407:Bacillus / Q000254:growth & development*; D002245:Carbon Dioxide; D000431:Ethanol; D006861:Hydrogen Peroxide; D013171:Spores, Bacterial / Q000254:growth & development; D013242:Sterilization / Q000295:instrumentation / Q000379:methods*
D002245:Carbon Dioxide; D000431:Ethanol; D006861:Hydrogen Peroxide; D019840:2-Propanol