[Interaction between RNA molecules of a two-componet trans-analog of antigenomic HDV-ribozyme].
Association of RNA molecules forming a two-component B:LS trans-analog of antigenomic HDV ribozyme was studied. From previously synthesized trans-ribozymes the B:LS ribozyme differs by length and sequence of its RNA molecules (33 and 34 bp, respectively), topology of functional parts and the absence of very short reaction product. The ribozyme displays a biphasic kinetics of self-cleavage similar to that of cis-ribozyme. Our original kinetic scheme for the B:LS trans-ribozyme self-cleavage (www.cardio.ru\labgen\RZ_e.html)describes a possible cause of biphasic nature of the reaction curve, namely, variation of the rate-limiting stage in the series of successive conformational transformations which coincide with the ribozyme self-cleavage. Interactions between the molecules involved in the reaction, i.e., "multimerization" of entire ribozyme and its components can be regarded as another cause of the biphasic kinetics. B:LS trans-ribozyme is a convenient model for the investigation of this process, since the binding of LS and B allows the formation of complexes with 1B:2LS or 2B:1LS stoichiometry and complexes with the cleavage products. We examined the factors determining dissociation-association of the ribozyme components using a series of electrophoreses under nondenaturing conditions. The possibility of interaction between cis- and transribozyme components was confirmed experimentally. In the presence of LS excess over B the ribozyme can form multimeres. These findings suggest the involvement of intermolecular interactions in native cis-ribozyme self-cleavage.
Alekseenkova
V A
VA
Belianko
T I
TI
Lukin
M A
MA
Savochkina
L P
LP
Bibilashvili
R Sh
RSh
D020029:Base Pairing; D001483:Base Sequence; D016679:Genome, Viral; D003698:Hepatitis Delta Virus / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry*; D012367:RNA, Viral / Q000235:genetics*; D013329:Structure-Activity Relationship
D016337:RNA, Catalytic; D012367:RNA, Viral