[Screening differentially expressed genes in denucleated K562 cells with restriction display technique].
OBJECTIVE
To screen differentially expressed genes in cytochalasin B (CB)-induced denucleated K562 cells by restriction display (RD) technique.


METHODS
The total RNA was isolated and purified from K562 cells before and after CB (10 mug/ml) treatment. The mRNA from both treated and untreated K562 cells were reversely transcribed into cDNA, and the differentially expressed genes were separated using RD technique combined with polyacrylamide gel electrophoresis and sliver staining, followed by cloning, sequencing and homology analysis against GenBank database of these genes.


RESULTS
Seven differentially expressed genes were identified in CB-treated cells including aquaporin 1 (AQP1) gene, which was verified to be up-regulated after CB treatment by RT-PCR.


CONCLUSIONS
AQP1 gene might be in close association with the regulation of denucleation processes and CB-induced proliferation inhibition of K562 cells.
Wei
Min
M
Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China. phenney@fimmu.com
Ma
Wen-li
WL
Song
Yan-bin
YB
Mao
Xiang-ming
XM
Li
Ling
L
Zheng
Wen-ling
WL
D051398:Aquaporin 1 / Q000096:biosynthesis / Q000235:genetics*; D003571:Cytochalasin B / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
C496020:AQP1 protein, human; D051398:Aquaporin 1; D003571:Cytochalasin B