Cellular uptake of phosphorothioate oligonucleotide facilitated by cationic porphyrin: a microfluorescence study.
Cationic 5,10,15,20-tetrakis (1-methyl-4-pyridyl) porphyrin was tested as a delivery agent for oligonucleotides. By using fluorescence microimaging, it has been shown that complexation of the porphyrin to the phosphorothioate analog of dT(15) labeled by rhodamine enabled its nonendocytic penetration into the cell and regular distribution in the cytoplasm and preferentially into the nucleus. Time-resolved microfluorescence spectroscopy revealed that the oligonucleotide integrity was kept. A small fraction of the porphyrin molecules seems to undergo change of the binding mode after internalization, probably due to duplex formation between the oligonucleotide and its cellular target sequences, or due to dissociation of the porphyrin from the oligonucleotide and subsequent interactions in the cellular environment.
Kocisová
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E
Institute of Physics, Charles University, Ke Karlovu 3, 2 Prague 12116, Czech Republic. kocisova@karlov.mff.cuni.cz
Praus
P
P
Mojzes
P
P
Sureau
F
F
Stepánek
J
J
Seksek
O
O
Turpin
P Y
PY
D016475:3T3 Cells; D000818:Animals; D051379:Mice; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000378:metabolism*; D011166:Porphyrins / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D016376:Oligonucleotides, Antisense; D011166:Porphyrins