Consecutive use of two multiplex PCR-based assays for simultaneous identification and determination of capsular status of nine common Neisseria meningitidis serogroups associated with invasive disease.
We developed two Neisseria meningitidis multiplex PCR assays to be used consecutively that allow determination of the serogroup and capsular status of serogroup A, B, C, 29E, W135, X, and Y cnl-3/cnl-1-like-containing N. meningitidis isolates by direct analysis of the amplicon size. These assays offer a rapid and simple method of serogrouping N. meningitidis.
Bennett
Désirée E
DE
Epidemiology and Molecular Biology Unit, The Children's University Hospital, Temple Street, Dublin 1, Ireland.
Cafferkey
Mary T
MT
D001483:Base Sequence; D002353:Carrier State / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008589:Meningococcal Infections / Q000382:microbiology; D009345:Neisseria meningitidis / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D038381:Neisseria meningitidis, Serogroup A / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D038541:Neisseria meningitidis, Serogroup B / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D038542:Neisseria meningitidis, Serogroup C / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D038582:Neisseria meningitidis, Serogroup W-135 / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D038581:Neisseria meningitidis, Serogroup Y / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods* / Q000706:statistics & numerical data; D014774:Virulence / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial