Human B-ind1 gene promoter: cloning and regulation by histone deacetylase inhibitors.
Histone deacetylase inhibitors (HDIs) induced expression of the B-ind1 protein that is a component of Rac-1-signaling pathways leading to the modulation of gene expression. In the present study, we have determined the structure of the human B-ind1 gene promoter region. The oligocapping method revealed that the transcriptional start site of the human B-ind1 gene is located at 166 bases upstream of the first adenine residue of the translation start site that is highly homologous to an initiator (Inr) consensus sequence. In reporter assays, transactivation of the B-ind1 promoter was observed up to 300 bp of the initiation site. Deletion analysis of the promoter region revealed that histone deacetylase inhibitors (HDIs)-induced luciferase response was regulated by the core promoter elements. Mutation introduced into the proximal CG-boxes decreased most of the basal and HDIs-induced promoter activity. These results suggested a novel mechanism, which implicate minimal core promoter elements as potential mediator of HDIs.
Sabbah
Michèle
M
Institut National de la Santé et de la Recherche Médicale U673, 184, rue du Faubourg Saint-Antoine, Hôpital Saint-Antoine, 75571 Paris cedex 12, France.
Saucier
Cécile
C
Redeuilh
Gérard
G
D024506:5' Flanking Region; D001483:Base Sequence; D001665:Binding Sites; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D017930:Genes, Reporter; D056572:Histone Deacetylase Inhibitors; D006801:Humans; D006836:Hydro-Lyases; D047908:Intracellular Signaling Peptides and Proteins; D008156:Luciferases / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D016329:Sp1 Transcription Factor / Q000737:chemistry; D024363:Transcription Initiation Site; D015533:Transcriptional Activation
D056572:Histone Deacetylase Inhibitors; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D016329:Sp1 Transcription Factor; D008156:Luciferases; C410722:HACD3 protein, human; D006836:Hydro-Lyases