title: Level of M(IP)2C sphingolipid affects plant defensin sensitivity, oxidative stress resistance and chronological life-span in yeast.
abstract: The antifungal plant defensin DmAMP1 interacts with fungal sphingolipids of mannosyldiinositolphosphorylceramide (M(IP)2C) class. We screened a Saccharomyces cerevisiae transposon (Tn) mutant library against DmAMP1 and identified one DmAMP1-resistant mutant with the Tn inserted in the M(IP)2C biosynthesis gene IPT1 (DmTn11) and one DmAMP1-hypersensitive mutant with the Tn inserted in rDNA (HsTnII). However, tetrad analysis pointed to HsTnII as a spontaneous mutant. Apparently, membranes of DmTn11 lack M(IP)2C, whereas membranes of HsTnII have increased M(IP)2C levels. In addition, DmTn11 and HsTnII are characterized by increased and reduced oxidative stress resistance/chronological life-span (CL), respectively. A putative involvement of M(IP)2C in oxidative stress and CL in yeast is discussed.
authors.affiliation: Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium.
authors.forename: An M
authors.initials: AM
authors.lastname: Aerts
authors.forename: Isabelle E J A
authors.initials: IE
authors.lastname: François
authors.forename: Leen
authors.initials: L
authors.lastname: Bammens
authors.forename: Bruno P A
authors.initials: BP
authors.lastname: Cammue
authors.forename: Bart
authors.initials: B
authors.lastname: Smets
authors.forename: Joris
authors.initials: J
authors.lastname: Winderickx
authors.forename: Sabina
authors.initials: S
authors.lastname: Accardo
authors.forename: Dirk E
authors.initials: DE
authors.lastname: De Vos
authors.forename: Karin
authors.initials: K
authors.lastname: Thevissen
mesh_terms: D023082:Defensins / Q000502:physiology*; D006028:Glycosphingolipids / Q000032:analysis / Q000502:physiology*; D009154:Mutation; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D010944:Plants / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics; D015003:Yeasts / Q000166:cytology*
chemical_list: D023082:Defensins; D006028:Glycosphingolipids; C068389:mannose-bis(inositolphospho)ceramide; D010100:Oxygen