title: Polychromatic LED in oval full-thickness wound healing in non-diabetic and diabetic rats.
abstract: OBJECTIVE
  Our goal was to determine the efficacy of polychromatic light-emitting diode (LED) in the enhancement of wound healing in non-diabetic and diabetic rats.
  
  
  BACKGROUND
  LEDs are increasingly used as an alternative light source for phototherapy.
  
  
  METHODS
  A cluster of 25 LED photons at 510-543, 594-599, 626-639, 640-670, and 842-872 nm wavelengths with 272-mW output power was used. Male Sprague-Dawley rats (n = 61) were randomly assigned into non-diabetic and diabetic groups and into light treatment groups, that is, control, 5, 10, 20, and 30 J/cm(2). Streptozotocin was used for diabetes induction. Wounds were created using a scalpel after 1 week of wounding. Wounds were measured daily and plotted in time, and the trendline was fitted to obtain slope values. Relative wound healing percentage was computed as follows: RWH% = [(Slope treated - Slope control)/(Slope control)] x 100. The t-test (alpha = 0.05) was used for analysis.
  
  
  RESULTS
  The RWH% in the non-diabetic rats was insignificant (p > 0.05) at 5, 10, 20, and 30 J/cm(2) treatments, giving 4.3 +/- 1.97%, 5.4 +/- 1.94%, 4.5 +/- 1.96%, and 1.2 +/- 2.03%, respectively. The healing of diabetic rats was significantly impaired by -11.7 +/- 3.25% (p = 0.02), which was mitigated by 5 J/cm(2) treatment (2.4 +/- 3.02%, (p) = 0.40) and 10 J/cm(2) treatment (-5.5 +/- 3.28%, p = 0.11). Diabetic wound healing using 20 J/cm(2) (-8.7 +/- 3.39%, p = 0.03) and 30 J/cm(2) (-10.90 +/- 1.97%, p = 0.01) affected significant inhibition.
  
  
  CONCLUSIONS
  The effect of polychromatic LED therapy in oval full-thickness wound-healing in the diabetic model with the use of 5 and 10 J/cm(2) is promising. Further studies to determine optimum dosimetry and efficacy of LEDs are recommended.
authors.affiliation: Laser Medicine Research Section, Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
authors.forename: Farouk A H
authors.initials: FA
authors.lastname: Al-Watban
authors.forename: Bernard L
authors.initials: BL
authors.lastname: Andres
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D008297:Male; D023421:Models, Animal; D010789:Phototherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000528:radiation effects*; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects*