Characterization of a novel polymeric scaffold for potential application in tendon/ligament tissue engineering.
Unlike braided fabrics, knitted scaffolds have been proven to favor deposition of collagenous connective tissue matrix, which is crucial for tendon/ligament reconstruction. But cell seeding of such scaffolds often requires a gel system, which is unstable in a dynamic situation, especially in the knee joint. This study developed a novel, biodegradable nano-microfibrous polymer scaffold by electrospinning PLGA nanofibers onto a knitted PLGA scaffold in order to provide a large biomimetic surface for cell attachment. Porcine bone marrow stromal cells were seeded onto either the novel scaffolds by pipetting a cell suspension (Group I) or the knitted PLGA scaffolds by immobilizing in fibrin gel (Group II). Cell attachment at 36 hours, cell proliferation and extracellular matrix synthesis at 1 week, and mechanical properties over 2 weeks were investigated. Cell attachment was comparable and cell proliferation was faster in Group I. Moreover, cellular function was more actively exhibited in Group I, as evident by the higher expression of collagen I, decorin, and biglycan genes. Thus, this novel scaffold, facilitating cell seeding and promoting cell proliferation, function, and differentiation, could be applied with promise in tissue engineering of tendon/ligament.
Sahoo
S
S
Tissue Repair Lab, Division of Bioengineering, National University of Singapore, Singapore.
Ouyang
H
H
Goh
James C-H
JC
Tay
T E
TE
Toh
S L
SL
D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000235:genetics; D019344:Lactic Acid; D008022:Ligaments / Q000648:ultrastructure; D008422:Materials Testing; D018613:Microscopy, Confocal; D049329:Nanostructures; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells; D013552:Swine; D013710:Tendons / Q000648:ultrastructure; D023822:Tissue Engineering
D001672:Biocompatible Materials; D016326:Extracellular Matrix Proteins; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid