Effects of subminimum inhibitory concentrations of antibiotics on the Pasteurella multocida proteome.
Subminimum inhibitory concentrations (sub-MICs) of antibiotics can be therapeutically effective, but the underlying molecular mechanisms are not well-characterized. We analyzed the Pasteurella multocida proteome response to sub-MICs of amoxicillin, chlortetracycline, and enrofloxacin using isotope-coded affinity tags (ICAT). There were parallel effects on inhibition of growth kinetics and suppression of protein expression by clusters of orthologous groups (COG) categories. Potential compensatory mechanisms enabling antibiotic adaptation were identified, including increased RecA expression caused by enrofloxacin.
Nanduri
Bindu
B
College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi 39762, USA.
Lawrence
Mark L
ML
Boyle
Carolyn R
CR
Ramkumar
Mahalingam
M
Burgess
Shane C
SC
D000658:Amoxicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002751:Chlortetracycline / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000494:pharmacology; D016979:Pasteurella multocida / Q000187:drug effects* / Q000254:growth & development; D014176:Protein Biosynthesis / Q000187:drug effects; D020543:Proteome / Q000187:drug effects / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D020543:Proteome; D000077422:Enrofloxacin; D000658:Amoxicillin; D002751:Chlortetracycline