title: Aberrant maspin expression in gallbladder epithelium is associated with intestinal metaplasia in patients with cholelithiasis.
abstract: OBJECTIVE
  Aberrant expression of maspin protein related to DNA hypomethylation in the promoter region is frequently observed in gallbladder carcinomas, whereas the non-tumorous gallbladder epithelium is maspin negative. We investigated maspin expression in non-tumorous gallbladder epithelium in patients with cholelithiasis.
  
  
  METHODS
  An immunohistochemical study of maspin expression was performed in 69 patients with cholelithiasis and 30 patients with gastric cancer without cholelithiasis.
  
  
  RESULTS
  Immunoreactivity for maspin was observed in focal and patchy regions of the gallbladder epithelium. Positive immunoreactivity for maspin was significantly associated with the presence of intestinal metaplasia in patients with cholelithiasis (p<0.05).
  
  
  CONCLUSIONS
  The high incidence of aberrant maspin expression in both intestinal metaplasia and carcinoma of the gallbladder supports the assumption that intestinal metaplasia of the gallbladder may predispose to gallbladder carcinoma.
authors.affiliation: Department of Pathology, Iwate Medical University School of Medicine, Uchimaru 19-1, 020-8505 Morioka, Japan. chihaya@iwate-med.ac.jp
authors.forename: C
authors.initials: C
authors.lastname: Maesawa
authors.forename: S
authors.initials: S
authors.lastname: Ogasawara
authors.forename: A
authors.initials: A
authors.lastname: Yashima-Abo
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Kotani
authors.forename: S
authors.initials: S
authors.lastname: Masuda
authors.forename: Y
authors.initials: Y
authors.lastname: Nagata
authors.forename: T
authors.initials: T
authors.lastname: Iwaya
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Oyake
authors.forename: Y
authors.initials: Y
authors.lastname: Akiyama
authors.forename: H
authors.initials: H
authors.lastname: Kawamura
authors.forename: T
authors.initials: T
authors.lastname: Masuda
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D016022:Case-Control Studies; D002769:Cholelithiasis / Q000737:chemistry* / Q000473:pathology; D018450:Disease Progression; D004727:Endothelium / Q000737:chemistry / Q000473:pathology; D005260:Female; D005704:Gallbladder / Q000737:chemistry* / Q000473:pathology; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D008679:Metaplasia; D008875:Middle Aged; D015843:Serpins / Q000032:analysis*; D013274:Stomach Neoplasms / Q000737:chemistry
chemical_list: D014408:Biomarkers, Tumor; C085344:SERPIN-B5; D015843:Serpins