title: Meeting report: Application of genotyping methods to assess risks from cryptosporidium in watersheds.
abstract: A workshop titled "Application of Genotyping Methods to Assess Pathogen Risks from Cryptosporidium in Drinking Water Catchments" was held at the International Water Association biennial conference, Marrakech, Morocco, 23 September 2004. The workshop presented and discussed the findings of an interlaboratory trial that compared methods for genotyping Cryptosporidium oocysts isolated from feces. The primary goal of the trial and workshop was to assess the utility of current Cryptosporidium genotyping methods for determining the public health significance of oocysts isolated from feces in potable-water-supply watersheds. An expert panel of 16 watershed managers, public health practitioners, and molecular parasitologists was assembled for the workshop. A subordinate goal of the workshop was to educate watershed management and public health practitioners. An open invitation was extended to all conference delegates to attend the workshop, which drew approximately 50 interested delegates. In this report we summarize the peer consensus emerging from the workshop. Recommendations on the use of current methods by watershed managers and public health practitioners were proposed. Importantly, all the methods that were reported in the trial were mutually supporting and found to be valuable and worthy of further utility and development. Where there were choices as to which method to apply, the small-subunit ribosomal RNA gene was considered to be the optimum genetic locus to target. The single-strand conformational polymorphism method was considered potentially the most valuable for discriminating to the subtype level and where a large number of samples were to be analyzed. A research agenda for protozoan geneticists was proposed to improve the utility of methods into the future. Standardization of methods and nomenclature was promoted.
authors.affiliation: Ecowise Environmental, Canberra, Australia. cferguson@ecowise.com.au
authors.forename: Christobel
authors.initials: C
authors.lastname: Ferguson
authors.forename: Dan
authors.initials: D
authors.lastname: Deere
authors.forename: Martha
authors.initials: M
authors.lastname: Sinclair
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Chalmers
authors.forename: Kristin
authors.initials: K
authors.lastname: Elwin
authors.forename: Stephen
authors.initials: S
authors.lastname: Hadfield
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
authors.forename: Una
authors.initials: U
authors.lastname: Ryan
authors.forename: Robin
authors.initials: R
authors.lastname: Gasser
authors.forename: Youssef Abs
authors.initials: YA
authors.lastname: El-Osta
authors.forename: Melita
authors.initials: M
authors.lastname: Stevens
mesh_terms: D000818:Animals; D003458:Cryptosporidium / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006801:Humans; D033661:Oocysts / Q000235:genetics / Q000302:isolation & purification; D018807:Polymorphism, Single-Stranded Conformational; D018570:Risk Assessment; D014881:Water Supply