A rapid LC-MS method for determination of plasma anion profiles of acidotic patients.
In metabolic acidosis, the concentrations of anions associated with intermediary metabolism are increased and can make a significant contribution to the observed acidosis. Here we describe a method for the rapid determination of the plasma ultrafiltrate profile of these anions using liquid chromatography coupled to electrospray ionisation mass spectrometry (LC/ESI-MS). The ultrafiltrate from patients with acidosis resulting from various causes were examined and the results compared to control values. Using the LC/ESI-MS method described, a unique plasma ultrafiltrate anion profile was obtained for each of the groups studied that provides rapid diagnosis of the type of underlying acidosis.
McKinnon
William
W
Renal Research Laboratory, Department Medicine, 4th Floor, North Wing, St. Thomas' Hospital, Lambeth Palace Road, London SE1 7EH, UK.
Lord
Gwyn A
GA
Forni
Lui G
LG
Peron
Jean-Marie R
JM
Hilton
Philip J
PJ
D000138:Acidosis / Q000097:blood*; D000838:Anions / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000838:Anions