Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv.
Fatty acid-CoA racemase plays an important role in the beta-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P3(1) or P3(2), with unit-cell parameters a = b = 109.56, c = 147.97 A. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 A3 Da(-1). A complete native data set has been collected at 2.7 A resolution using a synchrotron-radiation source.
Rhee
Kyung Hee
KH
Biomedical Research Center, Division of Life Sicence, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Sungbuk-gu, Seoul 136-791, South Korea.
Lee
Ki Seog
KS
Priyadarshi
Amit
A
Kim
Eunice Eunkyung
EE
Hwang
Kwang Yeon
KY
D042962:Acyl-CoA Oxidase / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D005227:Fatty Acids / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D019998:Racemases and Epimerases / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins; D013237:Stereoisomerism; D017356:Synchrotrons; D014961:X-Ray Diffraction
D005227:Fatty Acids; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; D042962:Acyl-CoA Oxidase; D019998:Racemases and Epimerases; D010100:Oxygen