title: [Differentiation of capripoxvirus species and strains by polymerase chain reaction].
abstract: A fast and simple method for capripoxvirus species identification has been developed. The method is based on multiplex polymerase chain reaction (MPCR) with species-specific primers and does not require nucleotide sequencing or restriction analysis of PCR products. To differentiate vaccine stains used in Russia and countries of the former Soviet Union from epizootic isolates of sheep pox virus, a method based on restriction analysis of the ankyrin-repeat protein gene fragment amplified by PCR has been developed. Being highly specific, both methods may be used for routine diagnosis of capripoxvirus-associated diseases.
authors.forename: E S
authors.initials: ES
authors.lastname: Orlova
authors.forename: A V
authors.initials: AV
authors.lastname: Shcherbakova
authors.forename: V I
authors.initials: VI
authors.lastname: Diev
authors.forename: V M
authors.initials: VM
authors.lastname: Zakharov
mesh_terms: D000818:Animals; D018151:Capripoxvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D016133:Polymerase Chain Reaction; D012756:Sheep / Q000821:virology; D014765:Viral Vaccines / Q000235:genetics
chemical_list: D017931:DNA Primers; D014765:Viral Vaccines