title: Determination of zearalenone in barley, maize and wheat flour, polenta, and maize-based baby food by immunoaffinity column cleanup with liquid chromatography: interlaboratory study.
abstract: An interlaboratory study was performed on behalf of the UK Food Standards Agency to evaluate the effectiveness of an affinity column cleanup liquid chromatography (LC) method for the determination of zearalenone (ZON) in a variety of cereals and cereal products at proposed European regulatory limits. The test portion is extracted with acetonitrile:water. The sample extract is filtered, diluted, and applied to an affinity column. The column is washed, and ZON is eluted with acetonitrile. ZON is quantified by reversed-phase LC with fluorescence detection. Barley, wheat and maize flours, polenta, and a maize-based baby food naturally contaminated, spiked, and blank (very low level) were sent to 28 collaborators in 9 European countries and 1 collaborator in New Zealand. Participants were asked to spike test portions of all samples at a ZON concentration equivalent to 100 microg/kg. Average recoveries ranged from 91-111%. Based on results for 4 artificially contaminated samples (blind duplicates) and 1 naturally contaminated sample (blind duplicate), the relative standard deviation for repeatability (RSDr) ranged from 6.9-35.8%, and the relative standard deviation for reproducibility (RSDR) ranged from 16.4-38.2%. The method showed acceptable within- and between-laboratory precision for all 5 matrixes, as evidenced by HorRat values <1.7.
authors.affiliation: Central Science Laboratory, Sand Hutton, York, YO41 1LZ, United Kingdom. s.macdonald@csl.gov.uk
authors.forename: Susan J
authors.initials: SJ
authors.lastname: MacDonald
authors.forename: Sharron
authors.initials: S
authors.lastname: Anderson
authors.forename: Paul
authors.initials: P
authors.lastname: Brereton
authors.forename: Roger
authors.initials: R
authors.lastname: Wood
authors.forename: Andrew
authors.initials: A
authors.lastname: Damant
mesh_terms: D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods; D002853:Chromatography, Liquid; D005433:Flour / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D001467:Hordeum / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D008856:Microscopy, Fluorescence; D015233:Models, Statistical; D008970:Molecular Weight; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014908:Triticum / Q000378:metabolism*; D003313:Zea mays / Q000378:metabolism*; D015025:Zearalenone / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D015025:Zearalenone