On-chip micellar electrokinetic chromatographic separation of phenolic chemicals in waters.
This paper describes on-chip micellar electrokinetic chromatography (MEKC) separation of bisphenol A and 3 kinds of alkylphenols, which have been recently recognized as endocrine disrupting chemicals for fish by the Japanese government, using microchip capillary electrophoresis with UV detection. We successfully obtained high-speed separation of the phenolic chemicals within 15 s as optimizing in microfluidic controls and MEKC separation conditions. We obtained fairly good linearity with correlation coefficient of over 0.98 from 0 to 50 mg/l phenolic chemicals except for 4-nonylphenol, which sample is the mixture of many geometrical isomers (r = 0.86). The values of the relative standard deviation for peak height in 50 mg/l phenolic chemicals were less than 8% except for bisphenol A (11.0%). The limits of detection obtained at a signal-to-noise ratio of 3 were from 5.6 to 20.0 mg/l. To realize on-site monitoring, we described strategy for on-chip MEKC analysis of the phenolic chemicals in waters using a portable analyzer based on microfluidic devices.
Wakida
Shin-ichi
S
Human Stress Signal Research Center (HSS), National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan. s.wakida@aist.go.jp
Fujimoto
Kazushi
K
Nagai
Hidenori
H
Miyado
Takashi
T
Shibutani
Yasuhiko
Y
Takeda
Sahori
S
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D052244:Endocrine Disruptors / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis*
D052244:Endocrine Disruptors; D010636:Phenols; D014874:Water Pollutants, Chemical