[Specific coalescent peptide of CD59 screened by phage library].
OBJECTIVE
To screen and identify the short-peptide which specifically bind to human CD59 so as to design short-peptide clamp with counteracting tumor escape activity.


METHODS
Using CHO cells which high express human CD59 as target cells, the phage 12 peptide library was screened for 5 rounds by competitive binding test. After five rounds of screening and competitive binding test, positive phage clones were screened by ELISA and sequenced.


RESULTS
8 out of 16 phage clones which were chosen randomly were identified to have high binding power to CD59. After sequencing, 3 high homologous amino acids were obtained. DNAstar analysis showed that 3 sequences had some homology with the sequence of human CD2 published by PubMed.


CONCLUSIONS
The obtained sequence HxAxxxxxxPxx is helpful for design of short-peptide clamp of active sites of CD59 related to tumor escape.
Cheng
Ying
Y
Department of Immunology, Medical College of Qingdao University, Qingdao 266021, China. chengying66@sina.com
Gao
Mei-hua
MH
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D018957:CD59 Antigens / Q000737:chemistry* / Q000378:metabolism; D016466:CHO Cells; D020134:Catalytic Domain; D006224:Cricetinae; D003412:Cricetulus; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D019139:Tumor Escape / Q000187:drug effects*
D018957:CD59 Antigens; D019151:Peptide Library; D010455:Peptides