[HLA-DPB1 gene analysis in haematopoietic stem cell transplantations].
BACKGROUND
The HLA-DPB1 gene is probably one of HLA class II genes affecting the haematopoietic stem cell transplantation. The aim of the study was to analyse the HLA-DPB1 gene and its match/mismatch in patients transplanted from unrelated HSC donors. The PCR-SSP method was used for the typing of the HLA system.


RESULTS
The cohort consisted of 201 pairs of patient/unrelated HSC donor. Match in HLA-A, -B, -Cw, -DRBI and -DQBI (e.g. 10/10 match) was found in 81 pairs. The HLA-DPBI was tested in them. 18 different HLA-DPBI alleles were identified in this cohort. Complete match (e.g.12/12) was detected in 3% of the 201 analysed pairs only.


CONCLUSIONS
The probability of finding 12/12 matching unrelated HSC donor is limited due to the high percentage of mismatches and inaccessibility of previous HLA-DPB1 results.
Vraná
M
M
Ustav hematologie a krevní transfuze, Praha. milena.vrana@uhkt.cz
Dobrovolná
M
M
Cetkovský
P
P
Nazarová
S
S
Vondrácková
H
H
Sedlácek
P
P
D000328:Adult; D002648:Child; D005787:Gene Frequency; D006682:HLA-DP Antigens / Q000235:genetics*; D059868:HLA-DP beta-Chains; D018380:Hematopoietic Stem Cell Transplantation; D006650:Histocompatibility Testing; D006801:Humans; D014019:Tissue Donors
D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen