[Identification of sarcosaphagous flies by analyses the sequence of 16S rDNA].
OBJECTIVE
To solve the difficulties of identification of Sarcosaphagous flies such as Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) which could not be identified by analyzing the 278bp and 635 bp regions of the gene encoding for cytochrome oxidase subunit I and II (CO I and CO II) in mtDNA.


METHODS
Specimens were collected from the corpses of rabbits on the grassland in Huhhot and Chengdu, the sequences of 551 bp region of 16S rDNA of their mtDNA were analyzed, the multiple-alignment program DNAMAN(version 4.0) and MEGA 2.1 sofeware were employed for sequence alignments neighbour-joining tree construction.


RESULTS
Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) were distinguished successfully by sequence analysis of The 551 bp region of the gene of 16S rDNA.


CONCLUSIONS
The 551 bp region of the gene of 16S rDNA of sarcosaphagous flies can be used for identifying them on species level effectively. It is likely to be a successful compliment to identify the sarcosaphagous flies by sequence analysis of CO I and CO II in mtDNA.
Sun
Xiao-ming
XM
Institute of Forensic Medicine, Sichuan University, Chengdu 610041, China. lupusstar@163.com
Cai
Ji-feng
JF
Ying
Bin-wu
BW
Fang
Yue-qin
YQ
Yun
Li-bing
LB
Yuan
Wan-an
WA
Gao
Shan
S
Hou
Yi-ping
YP
D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D004175:Diptera / Q000145:classification / Q000235:genetics*; D005554:Forensic Medicine / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D011817:Rabbits; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S