title: No evidence of West Nile virus infection in Dutch blood donors.
abstract: OBJECTIVE
  West Nile virus (WNV) can be transmitted by transfusion through infected blood components. This study aimed to determine the prevalence of WNV infection among Dutch blood donors to assess whether WNV is a possible threat for the Dutch blood supply.
  
  
  METHODS
  Plasma samples from 61 992 blood donations were pooled in 7,749 test pools of eight donations using a Tecan robot. These samples were collected between April and October 2004. The pools were tested for the presence of WNV RNA by using the Procleix WNV assay.
  
  
  RESULTS
  No WNV RNA-positive pools were detected. Based on Poisson distribution statistics, extrapolation of our data to all the Dutch donations in 2004 revealed that between 0 and 55 cases of WNV infection could be expected.
  
  
  CONCLUSIONS
  No evidence of the presence of WNV RNA in Dutch blood donor samples from 2004 was found. However, surveillance of this emerging infection is of importance to safeguard the blood supply in the future because the transmission cycle of WNV is complex and hard to predict.
authors.affiliation: Sanquin Diagnostic Services, Viral Diagnostic Services Department, Amsterdam, the Netherlands. m.koppelman@sanquin.nl
authors.forename: M H G M
authors.initials: MH
authors.lastname: Koppelman
authors.forename: M S
authors.initials: MS
authors.lastname: Sjerps
authors.forename: M
authors.initials: M
authors.lastname: de Waal
authors.forename: H W
authors.initials: HW
authors.lastname: Reesink
authors.forename: H T M
authors.initials: HT
authors.lastname: Cuypers
mesh_terms: D001771:Blood Banks; D001782:Blood Donors; D001803:Blood Transfusion; D005260:Female; D006801:Humans; D008297:Male; D009426:Netherlands; D010949:Plasma / Q000821:virology; D012367:RNA, Viral / Q000097:blood*; D012189:Retrospective Studies; D014901:West Nile Fever / Q000097:blood* / Q000453:epidemiology / Q000635:transmission; D014902:West Nile virus
chemical_list: D012367:RNA, Viral