Crystallization and preliminary X-ray analysis of reducing-end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125.
The reducing-end xylose-releasing exo-oligoxylanase (Rex) from Bacillus halodurans C-125, a novel family GH8 glycoside hydrolase, was crystallized by the hanging-drop vapour-diffusion method using 13.6 mg ml(-1) purified Rex, 5.6%(v/v) polyethylene glycol 4000, 70 mM sodium acetate pH 4.6 and 30%(v/v) glycerol. Suitable crystals grew after incubation for 5 d at 293 K. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 52.69, b = 86.02, c = 87.92 A. X-ray diffraction data were collected at a resolution of 1.35 A.
Honda
Yuji
Y
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Fushinobu
Shinya
S
Hidaka
Masafumi
M
Wakagi
Takayoshi
T
Shoun
Hirofumi
H
Kitaoka
Motomitsu
M
D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D003460:Crystallization; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction; D014961:X-Ray Diffraction; D014994:Xylose / Q000378:metabolism*; D014995:Xylosidases / Q000737:chemistry* / Q000302:isolation & purification
D001426:Bacterial Proteins; D014994:Xylose; D014995:Xylosidases; C510191:reducing-end xylose-releasing exo-oligoxylanase, Bacillus halodurans; D043364:Endo-1,4-beta Xylanases