Scavenging of reactive oxygen species by Eriobotrya japonica seed extract.
We have clarified that Eriobotrya japonica seed extract has strong antioxidative activity, and is effective for the prevention and treatment of various diseases, such as hepatopathy and nephropathy. In this study, to investigate the influences of components of Eriobotrya japonica seed extract on its antioxidative activity, extracts were prepared using various solvents (n-hexane (Hex), ethyl acetate (EtOAc), n-butanol (n-BuOH), methanol (MeOH) and H2O) and the antioxidative activity of the solvent fractions and components was evaluated based on the scavenging of various radicals (DPPH and O2(-)) measured by the ESR method and the inhibition of Fe3+-ADP induced NADPH dependent lipid peroxidation in rat liver microsomes. The radical scavenging activities and inhibitory activities on lipid peroxidation differed among the solvent fractions and components. In the n-BuOH, MeOH and H2O fractions, radical scavenging activity and inhibitory activity on lipid peroxidation were high. In addition, these fractions contained abundant polyphenols, and the radical scavenging activity increased with the polyphenol content. In the low-polar Hex and EtOAc fractions, the radical scavenging activity was low, but the lipid peroxidation inhibition activity was high. These fractions contained beta-sitosterol, and the inhibitory activity on lipid peroxidation was high. Based on these findings, the antioxidative activity of Eriobotrya japonica seed extract may be derived from many components involved in a complex mechanism, resulting in high activity.
Yokota
Junko
J
Department of Biomedical Science, Kochi Medical Graduate School, Nankoku, Japan. yokotaj@med.kochi-u.ac.jp
Takuma
Daisuke
D
Hamada
Atsuhide
A
Onogawa
Masahide
M
Yoshioka
Saburo
S
Kusunose
Masahiko
M
Miyamura
Mitsuhiko
M
Kyotani
Shojiro
S
Nishioka
Yutaka
Y
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001713:Biphenyl Compounds; D004578:Electron Spin Resonance Spectroscopy; D031983:Eriobotrya / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007501:Iron / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D016877:Oxidants / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D012639:Seeds / Q000737:chemistry; D012855:Sitosterols / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D016877:Oxidants; D010851:Picrates; D010936:Plant Extracts; D012855:Sitosterols; D013481:Superoxides; D009249:NADP; C025473:gamma-sitosterol; D000244:Adenosine Diphosphate; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron