Purification, crystallization and preliminary X-ray diffraction analysis of the human major histocompatibility antigen HLA-B*2703 complexed with a viral peptide and with a self-peptide.
The product of the human leukocyte antigen (HLA) gene HLA-B*2703 differs from that of the prototypical subtype HLA-B*2705 by a single amino acid at heavy-chain residue 59 that is involved in anchoring the peptide N-terminus within the A pocket of the molecule. Two B*2703-peptide complexes were crystallized using the hanging-drop vapour-diffusion method using PEG 8000 as a precipitant. The crystals belong to space group P2(1) (pVIPR peptide) or P2(1)2(1)2(1) (pLMP2 peptide). Data sets were collected to 1.55 A (B*2703-pVIPR) or 2.0 A (B*2703-pLMP2) resolution using synchrotron radiation. With B*2705-pVIPR as a search model, a clear molecular-replacement solution was found for both B*2703 complexes.
Loll
Bernhard
B
Institut für Chemie/Kristallographie, Freie Universität Berlin, Takustrasse 6, 14195 Berlin, Germany.
Zawacka
Anna
A
Biesiadka
Jacek
J
Rückert
Christine
C
Volz
Armin
A
Saenger
Wolfram
W
Uchanska-Ziegler
Barbara
B
Ziegler
Andreas
A
D000936:Antigen-Antibody Complex / Q000737:chemistry*; D000956:Antigens, Viral / Q000737:chemistry; D001324:Autoantigens / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D015235:HLA-B Antigens / Q000737:chemistry* / Q000276:immunology; D015796:HLA-B27 Antigen; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D014764:Viral Proteins / Q000737:chemistry; D014835:Volatilization; D014961:X-Ray Diffraction
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D001324:Autoantigens; D015235:HLA-B Antigens; C510618:HLA-B*27:03 antigen; D015796:HLA-B27 Antigen; D010446:Peptide Fragments; D014764:Viral Proteins