title: Mold infestation of wet spray-applied cellulose insulation.
abstract: Mold investigations were conducted in four buildings that had been insulated with wet spray-applied cellulose insulation (WSACI). Bulk WSACI samples were collected and analyzed by quantitative polymerase chain reaction (QPCR) methods. Airborne mold was evaluated using both Burkard total mold spore and Andersen culturable/viable sampling methods. Although reportedly treated with biocidal borates, QPCR analyses indicated that elevated concentrations of mold cells (reported as spore equivalents per gram) may be present in WSACI. QPCR analyses showed the following: (1) very high concentrations of Penicillium chrysogenum in samples from two of four buildings; (2) very high concentrations of Stachybotrys chartarum in samples from one building and a more moderate presence in a second; (3) moderately high concentrations of Aspergillus versicolor in samples from one building and more moderate concentrations in a second; (4) the presence of the opportunistic pathogen, Aspergillus fumigatus, in samples from three of the four buildings, and (5) the presence of 22 of 23 target mold species. Elevated airborne total mold spore concentrations were observed in all four of the buildings investigated. Culturable/viable airborne mold concentrations were moderately elevated in three of the four buildings. Mold genera/types present were relatively consistent among airborne mold samples collected by both methods and bulk sample analyses. Results of this study suggest that WSACI has the potential to cause elevated airborne mold levels in buildings where it has been applied and pose significant mold exposure and public health risks.
authors.affiliation: Department of Natural Resources and Environmental Management, Ball State University, Muncie, IN 47306, USA.
authors.forename: Thad J
authors.initials: TJ
authors.lastname: Godish
authors.forename: Diana R
authors.initials: DR
authors.lastname: Godish
mesh_terms: D000391:Air Microbiology; D016902:Air Pollution, Indoor / Q000032:analysis*; D002482:Cellulose; D003252:Construction Materials / Q000382:microbiology*; D004784:Environmental Monitoring; D005160:Facility Design and Construction; D005658:Fungi / Q000145:classification; D006798:Housing; D017132:Workplace
chemical_list: D002482:Cellulose