From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags were identified and used to raise monospecific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL compared with normal lymphoid tissues. We propose that genome-based, affinity proteomics, using protein epitope signature tag-induced antibodies, is an efficient way to rapidly identify a number of disease-associated protein candidates of both previously known and unknown identities.
Ek
Sara
S
Department of Immunotechnology, Lund University, SE-22007 Lund, Sweden. sara.ek@immun.lth.se
Andréasson
Ulrika
U
Hober
Sophia
S
Kampf
Caroline
C
Pontén
Fredrik
F
Uhlén
Mathias
M
Merz
Hartmut
H
Borrebaeck
Carl A K
CA
D000912:Antibodies, Neoplasm; D016022:Case-Control Studies; D002648:Child; D000939:Epitopes; D018985:Epitopes, B-Lymphocyte; D006801:Humans; D008221:Lymphoid Tissue / Q000737:chemistry / Q000166:cytology; D020522:Lymphoma, Mantle-Cell / Q000737:chemistry* / Q000235:genetics* / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D040901:Proteomics / Q000379:methods*; D046888:Tissue Array Analysis
D000912:Antibodies, Neoplasm; D000939:Epitopes; D018985:Epitopes, B-Lymphocyte; D009363:Neoplasm Proteins