title: Isolation, crystallization and preliminary X-ray analysis of a methanol-induced corrinoid protein from Moorella thermoacetica.
abstract: A corrinoid protein was induced and overexpressed in methanol-grown cells of the thermophilic anaerobic bacterium Moorella thermoacetica. The protein was purified from cytosolic extracts. After screening for crystallization conditions and optimization, crystals were obtained that diffracted strongly on a rotating-anode X-ray source. A diffraction data set was collected and processed including reflections to 1.9 A resolution. Reflections were indexed in a primitive orthorhombic cell with unit-cell parameters a = 55.69, b = 62.74, c = 34.54 A. N-terminal amino-acid sequencing indicates that the crystals contain a C-terminal fragment of the protein.
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhou
authors.forename: Amaresh
authors.initials: A
authors.lastname: Das
authors.forename: Jeff E
authors.initials: JE
authors.lastname: Habel
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Jessie
authors.initials: J
authors.lastname: Chang
authors.forename: Lirong
authors.initials: L
authors.lastname: Chen
authors.forename: Doowon
authors.initials: D
authors.lastname: Lee
authors.forename: Duong
authors.initials: D
authors.lastname: Nguyen
authors.forename: Shu-Huey
authors.initials: SH
authors.lastname: Chang
authors.forename: Wolfram
authors.initials: W
authors.lastname: Tempel
authors.forename: John P
authors.initials: JP
authors.lastname: Rose
authors.forename: Lars G
authors.initials: LG
authors.lastname: Ljungdahl
authors.forename: Bi-Cheng
authors.initials: BC
authors.lastname: Wang
mesh_terms: D000105:Acetyl Coenzyme A / Q000096:biosynthesis / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D045728:Corrinoids / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006094:Gram-Positive Bacteria / Q000201:enzymology* / Q000378:metabolism; D000432:Methanol / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D045728:Corrinoids; D000105:Acetyl Coenzyme A; D000432:Methanol