title: Crystallization of the FaeE chaperone of Escherichia coli F4 fimbriae.
abstract: F4 (formerly K88) fimbriae from enterotoxigenic Escherichia coli are assembled via the FaeE/FaeD chaperone/usher pathway. The chaperone FaeE crystallizes in three crystal forms, all belonging to space group C2. Crystals of form 1 diffract to 2.3 A and have unit-cell parameters a = 195.7, b = 78.5, c = 184.6 A, beta = 102.2 degrees. X-ray data for crystal form 2 were collected to 2.7 A using an SeMet variant of FaeE. The crystals have unit-cell parameters a = 136.4, b = 75.7, c = 69.4 A, beta = 92.8 degrees. Crystals of form 3 were formed in a solution containing the FaeE-FaeG complex and diffract to 2.8 A. Unit-cell parameters are a = 109.7, b = 78.6, c = 87.8 A, beta = 96.4 degrees.
authors.affiliation: Laboratorium voor Ultrastructuur, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussel, Belgium. ivmolle@vub.ac.be
authors.forename: Inge
authors.initials: I
authors.lastname: Van Molle
authors.forename: Lieven
authors.initials: L
authors.lastname: Buts
authors.forename: Fanny
authors.initials: F
authors.lastname: Coppens
authors.forename: Liu
authors.initials: L
authors.lastname: Qiang
authors.forename: Lode
authors.initials: L
authors.lastname: Wyns
authors.forename: Remy
authors.initials: R
authors.lastname: Loris
authors.forename: Julie
authors.initials: J
authors.lastname: Bouckaert
authors.forename: Henri
authors.initials: H
authors.lastname: De Greve
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D029968:Escherichia coli Proteins / Q000737:chemistry*; D010861:Fimbriae, Bacterial / Q000737:chemistry; D018832:Molecular Chaperones / Q000737:chemistry*; D046912:Multiprotein Complexes / Q000737:chemistry; D012645:Selenomethionine; D014961:X-Ray Diffraction
chemical_list: D018830:Adhesins, Escherichia coli; D029968:Escherichia coli Proteins; C494388:FaeG protein, E coli; D018832:Molecular Chaperones; D046912:Multiprotein Complexes; C070012:faeE protein, E coli; D012645:Selenomethionine