Pulsed-field gel electrophoresis as a discriminatory typing technique for the biothreat agent burkholderia mallei.
Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) was used to type 21 laboratory strains of Burkholderia mallei. We demonstrated good resolution by PFGE together with clustering of some geographically related isolates, and confirmed previous observations that B. mallei is clonal as defined by MLST.
Chantratita
Narisara
N
Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Vesaratchavest
Mongkol
M
Wuthiekanun
Vanaporn
V
Tiyawisutsri
Rachaneeporn
R
Ulziitogtokh
Tsedev
T
Akcay
Erhan
E
Day
Nicholas P J
NP
Peacock
Sharon J
SJ
D000483:Alleles; D000818:Animals; D023482:Bioterrorism; D042726:Burkholderia mallei / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005896:Glanders / Q000382:microbiology*; D006736:Horses; D006801:Humans; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012703:Serotyping
D004269:DNA, Bacterial