title: Expression of interleukin-8 receptors in patients with adenomyosis.
abstract: OBJECTIVE
  To investigate the expression of interleukin-8 (IL-8) receptors CXCR1 and CXCR2 in adenomyosis.
  
  
  METHODS
  Comparative immunohistochemical study.
  
  
  METHODS
  Academic medical center.
  
  
  METHODS
  Thirty women who had undergone hysterectomy and were proved histopathologically to have adenomyosis, and 27 women without adenomyosis who had a hysterectomy for nonendometrial pathology such as leiomyomata or benign ovarian cysts.
  
  
  METHODS
  Tissue sections were immunostained with murine monoclonal anti-human CXCR1 and CXCR2 antibodies.
  
  
  METHODS
  Microscopic evaluation to assess the presence and localization of CXCR1 and CXCR2 throughout the menstrual cycle in both eutopic endometrial and adenomyotic tissues of women with adenomyosis and compare it with normal endometrium.
  
  
  RESULTS
  In eutopic endometrium of women with adenomyosis, proliferative phase samples showed higher epithelial CXCR1 and CXCR2 staining intensity compared with proliferative phase samples of normal endometrium. Adenomyosis foci expressed higher epithelial CXCR1 compared with the homologous eutopic endometrium and normal endometrium. On the other hand, adenomyosis foci and the homologous eutopic endometrium showed similar epithelial CXCR2 staining intensity, and this expression was higher than the normal controls.
  
  
  CONCLUSIONS
  Intrinsic abnormalities concerning IL-8 and its receptor system may be present in the eutopic endometrium of women affected by adenomyosis. These findings suggest that IL-8 receptors may be involved in the pathogenesis and/or pathophysiology of adenomyosis.
authors.affiliation: Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06520-8063, USA.
authors.forename: Murat
authors.initials: M
authors.lastname: Ulukus
authors.forename: E Cagnur
authors.initials: EC
authors.lastname: Ulukus
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Seval
authors.forename: Ozgur
authors.initials: O
authors.lastname: Cinar
authors.forename: Wenxin
authors.initials: W
authors.lastname: Zheng
authors.forename: Aydin
authors.initials: A
authors.lastname: Arici
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism; D005260:Female; D005498:Follicular Phase; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008875:Middle Aged; D023062:Receptors, Interleukin-8A / Q000378:metabolism*; D023063:Receptors, Interleukin-8B / Q000378:metabolism; D013194:Staining and Labeling; D014591:Uterine Diseases / Q000378:metabolism* / Q000473:pathology
chemical_list: D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B