title: Antibacterial effects of human group IIA and group XIIA phospholipase A2 against Helicobacter pylori in vitro.
abstract: Group IIA phospholipase A2 (PLA2-IIA) is an enzyme which has important roles in inflammation and infection. Recently, a novel human secretory PLA2 called group XIIA PLA2 (PLA2-XIIA) has been identified. Both PLA2-IIA and PLA2-XIIA are bactericidal against Gram-positive bacteria like many other secretory PLA2s. However, PLA2-XIIA is the only known PLA2 displaying significant bactericidal activity against the Gram-negative bacterium Escherichia coli. We examined the antibacterial properties of recombinant human PLA2-IIA and PLA2-XIIA against Helicobacter pylori, a Gram-negative bacterium, in vitro. PLA2-IIA was not bactericidal against H. pylori, whereas PLA2-XIIA effectively killed H. pylori at a concentration of 50 microg/ml but was not bactericidal at concentrations of 0.5 microg/ml and 5 microg/ml.
authors.affiliation: Department of Surgery, University of Turku, Finland. rajaa_ezzahiri@hotmail.com
authors.forename: Heikki T
authors.initials: HT
authors.lastname: Huhtinen
authors.forename: Juha O
authors.initials: JO
authors.lastname: Grönroos
authors.forename: Juha M
authors.initials: JM
authors.lastname: Grönroos
authors.forename: Jaakko
authors.initials: J
authors.lastname: Uksila
authors.forename: Michael H
authors.initials: MH
authors.lastname: Gelb
authors.forename: Timo J
authors.initials: TJ
authors.lastname: Nevalainen
authors.forename: V Jukka O
authors.initials: VJ
authors.lastname: Laine
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D054501:Group II Phospholipases A2; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori / Q000254:growth & development*; D006801:Humans; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D011994:Recombinant Proteins / Q000494:pharmacology; D013272:Stomach Diseases / Q000188:drug therapy* / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2