title: Cloning and expression of deoxyribonuclease II from chicken.
abstract: Acid endonucleases of the deoxyribonuclease II (DNase II, EC 3.1.22.1) family have been implicated in the degradation of DNA from apoptotic cell corpses formed in the process of normal mammalian development. Although a predicted DNase II has been detected in the chicken through expressed sequence tag (EST) analysis, to date no homolog of these important enzymes has been identified in vivo in any avian species. Here we report the cloning and expression of DNase II from the chicken, Gallus gallus. When expressed, the 363 amino acid glycoprotein is observed to be approximately 45 kDa in size and to exhibit DNA hydrolytic activity at pH 5 consistent with DNase II in other species. Furthermore, chicken DNase II sequence is compared with an identified partial sequence from the zebra finch, Taeniopygia guttata, as well as the previously identified homologs found in the fowlpox and canarypox viruses and the previously cloned mammalian DNases II. Through analysis of its amino acid sequence, comparative gene structure, and conserved synteny, chicken DNase II appears to represent a member of the DNase IIbeta subfamily and the apparent lack of a DNase IIalpha homolog in the chicken has important evolutionary implications for the study of this gene family.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Avian Disease and Oncology Laboratory, 3606 East Mount Hope Road, East Lansing, Michigan 48823, USA.
authors.forename: Kyle S
authors.initials: KS
authors.lastname: MacLea
authors.forename: Hans H
authors.initials: HH
authors.lastname: Cheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D004706:Endodeoxyribonucleases / Q000235:genetics*; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D015870:Gene Expression; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014162:Transfection
chemical_list: D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II