title: IL-10 polymorphism and cell-mediated immune response to Chlamydia trachomatis.
abstract: Chlamydia trachomatis infection induces an inflammatory response that is crucial in resolving acute infection but may also play a key role in the pathogenesis of C trachomatis associated infertility. The immune response is linked to cytokine secretion pattern which is influenced by the host genetic background. To study a relationship between interleukin-10 (IL-10) promoter -1082 polymorphism and cell-mediated immune response during C trachomatis infection in vitro, lymphocyte proliferation and cytokine (IL-10, IFN-gamma, TNF-alpha, IL-2, IL-4 and IL-5) secretion were analysed in subjects with different IL-10 genotypes. Enhanced IL-10 secretion and reduced antigen-specific lymphocyte proliferative and IFN-gamma responses were found in subjects with IL-10 -1082 GG genotype when compared to those with -1082 AA genotype. CD14+ monocytes were main source of IL-10 indicating that these cells are important regulators of the antigen-specific cell-mediated responses during active C trachomatis infection. We conclude that impaired cell-mediated response to C trachomatis is associated with IL-10 genotype in subjects with high IL-10 producing capacity. A comparison of immune markers between subjects with a history of noncomplicated and complicated infection is needed to further understand the confounding factors associated with the development of C trachomatis associated sequelae.
authors.affiliation: National Public Health Institute, Oulu, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Ohman
authors.forename: A
authors.initials: A
authors.lastname: Tiitinen
authors.forename: M
authors.initials: M
authors.lastname: Halttunen
authors.forename: S
authors.initials: S
authors.lastname: Birkelund
authors.forename: G
authors.initials: G
authors.lastname: Christiansen
authors.forename: P
authors.initials: P
authors.lastname: Koskela
authors.forename: M
authors.initials: M
authors.lastname: Lehtinen
authors.forename: J
authors.initials: J
authors.lastname: Paavonen
authors.forename: H-M
authors.initials: HM
authors.lastname: Surcel
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D002690:Chlamydia Infections / Q000235:genetics / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000235:genetics*; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D000907:Antibodies, Bacterial; D016207:Cytokines; D016753:Interleukin-10; D007371:Interferon-gamma