In vitro biosynthesis of the antitumor agent azinomycin B.
[reaction: see text] Azinomycins have potential therapeutic value as antitumor agents; however, their biosynthesis is poorly understood. Here, we provide the first demonstration of a protein cell-free system capable of supporting complete in vitro biosynthesis of the antitumor agent azinomycin B. The cell-free system is utilized to probe the cofactor dependence and substrate requirements of the pathway en route to azinomycin.
Liu
Chaomin
C
Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.
Kelly
Gilbert T
GT
Watanabe
Coran M H
CM
D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry; D002384:Catalysis; D036341:Intercellular Signaling Peptides and Proteins; D015394:Molecular Structure; D009281:Naphthalenes / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D013302:Streptomyces / Q000201:enzymology*
D000970:Antineoplastic Agents; D036341:Intercellular Signaling Peptides and Proteins; D009281:Naphthalenes; D010455:Peptides; C051055:azinomycin B