title: [In vivo comparison of preservation of murine islets of Langerhans].
abstract: OBJECTIVE
  A new preservation solution containing polyethyleneglycol (PEG) was studied. The length of the PEG chain determines its biological properties. The objective of this study was to define the optimal length of PEG chains to obtain the best tissue preservation.
  
  
  METHODS
  A murine model of islet of Langerhans transplantation was used. Solutions containing 8 kDa, 20 kDa or 35 kDa PEG at a molar concentration of 1.5 mM were compared to HBSS. Primary non-function (PNF) and mean graft survival rates were studied in these 4 groups.
  
  
  RESULTS
  Better preservation was obtained with 20 kDa PEG than with 8 kDa or 35 kDa (PNF rates of 0% vs 56% vs 37%, respectively). The difference with HBSS (22% PNF) was not significant. Graft survival was also longer with 20 kDa PEG than with 35 kDa PEG (23.1 +/- 4.4 days vs 8.6 +/- 7.6 days). Differences with HBSS (13.4 +/- 11.8 days) and 8 kDa PEG (12.2 +/- 14.7 days) were not significant.
  
  
  CONCLUSIONS
  The best preservation was obtained with 20 kDa PEG at a molar concentration of 1.5 mM. Other PEG concentrations need to be studied.
authors.affiliation: Faculté de Médecine Pitié-Salpêtrière, Université Pierre et Marie Curie (Paris VI), Laboratoire d'Immunologie Cellulaire et Tissulaire, INSERM U543, Paris, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Neuzillet
authors.forename: Sébastien
authors.initials: S
authors.lastname: Giraud
authors.forename: Lorraine
authors.initials: L
authors.lastname: Lagorce
authors.forename: Michel
authors.initials: M
authors.lastname: Eugène
authors.forename: François
authors.initials: F
authors.lastname: Richard
authors.forename: Patrice
authors.initials: P
authors.lastname: Debrè
authors.forename: Benoît
authors.initials: B
authors.lastname: Barrou
mesh_terms: D000818:Animals; D007515:Islets of Langerhans; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D019188:Organ Preservation Solutions
chemical_list: D019188:Organ Preservation Solutions