Lactulose and mannitol intestinal permeability detected by capillary electrophoresis.
Aim of this study was to set up a method by capillary electrophoresis to detect lactulose and mannitol in urine after an oral load, and to estimate the intestinal permeability in controls and in type I diabetes patients. The underivatized carbohydrates were monitored by indirect UV detection using sorbate, cetyltrimethylammonium bromide and LiOH as background electrolyte. Urines were purified by solid phase extraction, shaken with cation exchange resin, filtered and analysed. Carbohydrates migrated in <10 min in relation to their pK(a) and M(r). Controls (n = 33) and patients (n = 23) had an excretion ratio lactulose/mannitol 0.025 (0.018-0.051) and 0.067 (0.050-0.127), respectively (p < 0.01, median, interquartile range).
Paroni
Rita
R
Department of Medicine, Surgery and Dental Science, University of Milan, H San Paolo, via Di Rudini' 8, 20142 Milan, Italy. rita.paroni@unimi.it
Fermo
Isabella
I
Molteni
Laura
L
Folini
Laura
L
Pastore
Matteo Rocco
MR
Mosca
Andrea
A
Bosi
Emanuele
E
D000293:Adolescent; D000328:Adult; D002138:Calibration; D016022:Case-Control Studies; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007408:Intestinal Absorption; D007792:Lactulose / Q000493:pharmacokinetics* / Q000652:urine; D008353:Mannitol / Q000493:pharmacokinetics* / Q000652:urine
D008353:Mannitol; D007792:Lactulose