Osteogenic differentiation of human dental papilla mesenchymal cells.
We isolated dental papilla from impacted human molar and proliferated adherent fibroblastic cells after collagenase treatment of the papilla. The cells were negative for hematopoietic markers but positive for CD29, CD44, CD90, CD105, and CD166. When the cells were further cultured in the presence of beta-glycerophosphate, ascorbic acid, and dexamethasone for 14 days, mineralized areas together with osteogenic differentiation evidenced by high alkaline phosphatase activity and osteocalcin contents were observed. The differentiation was confirmed at both protein and gene expression levels. The cells can also be cryopreserved and, after thawing, could show in vivo bone-forming capability. These results indicate that mesenchymal type cells localize in dental papilla and that the cells can be culture expanded/utilized for bone tissue engineering.
Ikeda
Etsuko
E
National Institute of Advanced Industrial Science and Technology (AIST), Research Institute for Cell Engineering (RICE), Tissue Engineering Research Group, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan.
Hirose
Motohiro
M
Kotobuki
Noriko
N
Shimaoka
Hideki
H
Tadokoro
Mika
M
Maeda
Masahiko
M
Hayashi
Yoshiko
Y
Kirita
Tadaaki
T
Ohgushi
Hajime
H
D002113:Calcification, Physiologic / Q000502:physiology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002648:Child; D003771:Dental Papilla / Q000166:cytology* / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*