title: Internal Transcribed Spacer (ITS) of rDNA of appendaged and non-appendaged strains of Microsporum gypseum reveals Microsporum appendiculatum as its synonym.
abstract: Recently a new taxon of geophilic dermatophytes was established as Microsporum appendiculatum Bhat and Mariam, based on the presence of appendaged macroconidia. However, such appendages are already known in the related species Microsporum gypseum. We conducted a survey of soil in central India as a part of a microbial biodiversity project and obtained two strains of M. gypseum with appendaged macroconidia. Using phenotypical characterization in combination with sequencing and restriction fragment length polymorphism (RFLP) of the Internal Transcribed Spacer (ITS) region of rDNA, we found that all strains of appendaged species are identical. Therefore M. appendiculatum is regarded as a synonym of M. gypseum.
authors.affiliation: Mycological Research Laboratory, Department of Biological Sciences, Rani Durgavati University, Jabalpur 482001, MP, India. rahulxsharma@yahoo.co.in
authors.forename: Rahul
authors.initials: R
authors.lastname: Sharma
authors.forename: Ram C
authors.initials: RC
authors.lastname: Rajak
authors.forename: Akhilesh K
authors.initials: AK
authors.lastname: Pandey
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Gräser
mesh_terms: D004271:DNA, Fungal / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D007194:India; D008864:Microsporum / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012988:Soil Microbiology; D013045:Species Specificity; D009626:Terminology as Topic
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer