Simultaneous determination of key bioactive components in Hedyotis diffusa by capillary electrophoresis.
A capillary zone electrophoresis (CZE) method based on systematic one-variable-at-a time approach was developed for the analysis of four important bioactive components (geniposidic acid, ursolic acid, quercetin and p-coumaric acid) in the extract of Hedyotis diffusa (HD). Separations were carried out in a fused-silica capillary tube with peak detection at 214 nm. Good separation was achieved using a 20 mM borate buffer containing 5% acetonitrile as organic modifier and pH adjusted to 10.0. Operating voltage was 15 kV and temperature was maintained at 25 degrees C while hydrodynamic injection was 5s. A good linearity, with correlation coefficients in the ranges of 0.997-0.999 was obtained in the calibration curves of each standard. Relative standard deviation (R.S.D.) of migration time was between 0.32 and 0.70% and deviation of corrected peak area was between 8.84 and 11.99%. These results indicate that this method could be used for rapid and simultaneous analysis of the bioactive components in HD and other herbal products.
Cheung
H Y
HY
Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong. bhhonym@cityu.edu.hk
Cheung
S H
SH
Law
M L
ML
Lai
W P
WP
D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D032064:Hedyotis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results
D002021:Buffers