title: Recommendation for measuring and reporting chloride by ISEs in undiluted serum, plasma or blood.
abstract: The proposed recommendation for measuring and reporting chloride in undiluted plasma or blood by ion-selective electrodes (ISEs) will provide results that are identical to chloride concentrations measured by coulometry for standardized normal plasma or blood samples. It is applicable to all current ISEs dedicated to chloride measurement in undiluted samples that meet the requirements. However, in samples with reduced water concentration, results by coulometry are lower than by ion-selective electrode due to volume displacement. The quantity measured by this standardized ISE procedure is called the ionized chloride concentration. It may be clinically more relevant than the chloride concentration as determined by coulometry, photometry or by ISE after dilution of the sample.
authors.affiliation: Laboratory of Clinical Chemistry, National Institute of Nutrition, Bab Saadoun, Tunis, Tunisia.
authors.forename: Mohammed C Ben
authors.initials: MC
authors.lastname: Rayana
authors.forename: Robert W
authors.initials: RW
authors.lastname: Burnett
authors.forename: Arthur K
authors.initials: AK
authors.lastname: Covington
authors.forename: Paul
authors.initials: P
authors.lastname: D'Orazio
authors.forename: Niels
authors.initials: N
authors.lastname: Fogh-Andersen
authors.forename: Ellis
authors.initials: E
authors.lastname: Jacobs
authors.forename: Ritu
authors.initials: R
authors.lastname: Kataky
authors.forename: Wolf R
authors.initials: WR
authors.lastname: Külpmann
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kuwa
authors.forename: Lasse
authors.initials: L
authors.lastname: Larsson
authors.forename: Andrzej
authors.initials: A
authors.lastname: Lewenstam
authors.forename: Anton H J
authors.initials: AH
authors.lastname: Maas
authors.forename: Gerhard
authors.initials: G
authors.lastname: Mager
authors.forename: Jerzy W
authors.initials: JW
authors.lastname: Naskalski
authors.forename: Anthony O
authors.initials: AO
authors.lastname: Okorodudu
authors.forename: Christoph
authors.initials: C
authors.lastname: Ritter
authors.forename: Andrew
authors.initials: A
authors.lastname: St John
mesh_terms: D000704:Analysis of Variance; D002138:Calibration; D002712:Chlorides / Q000097:blood*; D004563:Electrochemistry; D006801:Humans; D007201:Indicator Dilution Techniques; D017736:Ion-Selective Electrodes; D010783:Photometry; D010949:Plasma / Q000737:chemistry*; D011188:Potassium / Q000097:blood*; D012016:Reference Values; D015203:Reproducibility of Results; D012964:Sodium / Q000097:blood*; D017186:Titrimetry
chemical_list: D002712:Chlorides; D012964:Sodium; D011188:Potassium