title: Spectrophotometric and electrical studies of charge-transfer complexes of sodium flucloxacillin with pi-acceptors.
abstract: The present study is interested to develop a simple, rapid and accurate spectrophotometric method for determination of sodium flucloxacillin (fluc) in pure form and pharmaceutical formulations. The charge-transfer (CT) interactions between sodium flucloxacillin as electron donor and chloranilic acid (CLA), dichloroquinone 4-chloroimide (DCQ), 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) and 7,7,8,8 tetracyano-p-quinodimethane (TCNQ), as pi-electron acceptors have been investigated spectrophotometrically. Different variables affecting the reaction were studied and optimized. Under the optimum conditions, linear relationships with good correlation coefficients (0.9979-0.9995) were found between the absorbance and the concentration of the drug in the range 16-880 microg ml(-1). The proposed methods were applied successfully to the determination of the examined drug either in pure or pharmaceutical dosage forms with good accuracy and precision. The formation of the CT-complexes and the sites of interaction were confirmed by elemental analysis CHN, UV-vis, IR, (1)H NMR and mass spectra techniques. Based on Job's method of continuous variation plots, the obtained results indicate the formation of 1:1 charge-transfer complexes with the general formula [(fluc)(acceptor)]. Statistical analysis of the obtained results showed no significant difference between the proposed method and official method.
authors.affiliation: Chemistry Department, Faculty of Education, Port-Said, Suez Canal University, Egypt.
authors.forename: Moamen S
authors.initials: MS
authors.lastname: Refat
authors.forename: Akram M
authors.initials: AM
authors.lastname: El-Didamony
mesh_terms: D000042:Absorption; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D005436:Floxacillin / Q000032:analysis* / Q000737:chemistry; D011809:Quinones / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D011809:Quinones; D005436:Floxacillin