Slower cycling of nestin-positive cells in neurosphere culture.
Neural stem cells are multipotent and self-renewing cells with important potential application in cell replacement therapy in brain damage. Many studies have shown that nestin-positive cells represent neural stem and progenitor cells in the central neural system. Here, we derived neural stem cells from the subventricular zone of a newborn nestin-promoter-driven green fluorescent protein mouse, and found that the percentage of nestin-positive cells decreased continuously at each passage in neurosphere culture. Using the relative proliferation ratio and relative division ratio analysis, we concluded that the slower cycling of nestin-positive cells was responsible for this decrease.
Ma
Bao-Feng
BF
Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou, China.
Liu
Xiao-Mei
XM
Xie
Xing-Mei
XM
Zhang
Ai-Xia
AX
Zhang
Jia-Qing
JQ
Yu
Wei-Hua
WH
Zhang
Xiu-Ming
XM
Li
Shu-Nong
SN
Lahn
Bruce T
BT
Xiang
Andy Peng
AP
D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D018874:Spheroids, Cellular; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors
D015415:Biomarkers; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D049452:Green Fluorescent Proteins