title: Involvement of brain-derived neurotrophic factor in the regulation of hypothalamic somatostatin in vivo.
abstract: Brain-derived neurotrophic factor (BDNF) has been extensively studied in the central nervous system as a survival and differentiation factor and in plasticity processes. In vitro, BDNF has been shown to stimulate cellular differentiation and neurohormones synthesis and release. We demonstrated that BDNF is a potent and specific stimulatory agent of somatostatin (SRIH) synthesis in primary cultures of hypothalamic neurons. However, less information is available about its function on SRIH neurons in vivo. In the present study, we examined the effect of in vivo intracerebroventricular BDNF administration in adult non-anesthetized male rats. Two distinct experimental approaches were used: acute intracerebroventricular injection and long-term (14 days) continuous infusion (Alzet micro-pumps). We demonstrate that single intracerebroventricular BDNF injections (5 microg/rat) induce an early (60 and 180 min) decrease in the SRIH mRNA signal in the hypothalamic periventricular nucleus (PeVN) accompanied by a decrease of the hypothalamic SRIH content. 48 h after the acute injection, SRIH mRNA levels and peptide content strongly and significantly increased. After continuous intracerebroventricular BDNF administration (12 microg/day for 14 days), a significant increase in the SRIH hypothalamic content was observed. Nevertheless, the increase in peptide content was not correlated with a similar increase in the PeVN messenger level. These findings show the involvement of BDNF in the in vivo regulation of somatostatinergic neurons in adult rats, which clearly differs according to the BDNF administration mode.
authors.affiliation: Molecular Mechanisms in Neurodegenerative Dementia Laboratory, U710 Inserm, University of Montpellier 2, EPHE, Place E Bataillon, 34095 Montpellier, France. lgivalois@univ-montp2.fr
authors.forename: Laurent
authors.initials: L
authors.lastname: Givalois
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Naert
authors.forename: Lucia
authors.initials: L
authors.lastname: Tapia-Arancibia
authors.forename: Sandor
authors.initials: S
authors.lastname: Arancibia
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007031:Hypothalamus / Q000737:chemistry* / Q000187:drug effects; D007091:Image Processing, Computer-Assisted; D017403:In Situ Hybridization / Q000379:methods; D007260:Infusion Pumps; D007276:Injections, Intraventricular; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013004:Somatostatin / Q000032:analysis / Q000235:genetics*; D013268:Stimulation, Chemical; D013997:Time Factors
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D013004:Somatostatin