Respiratory syncytial virus detection by Remel Xpect, Binax Now RSV, direct immunofluorescent staining, and tissue culture.
The performance characteristics of Xpect RSV (XP) and Binax Now RSV (BN) were compared to those of direct fluorescent-antibody staining and/or tissue culture for detection of respiratory syncytial virus (RSV) in nasopharyngeal aspirate and wash samples from children (n = 110) and adults (n = 66). The sensitivity, specificity, positive predictive value, and negative predictive value of XP were 75%, 98%, 95%, and 90%, respectively; and those of BN were 74%, 100%, 100%, and 90%, respectively. The performances of the assays were similar within a given age group and specimen type (nasopharyngeal aspirate or wash specimen). XP and BN are useful for screening for RSV in respiratory specimens when large volumes are tested or low levels of staffing occur.
Borek
Anita P
AP
Johns Hopkins Medical Institutions, Meyer Building, Room B1-193, 600 N. Wolfe St., Baltimore, Maryland 21287-7093, USA.
Clemens
Susan H
SH
Gaskins
Valerie K
VK
Aird
Deborah Z
DZ
Valsamakis
Alexandra
A
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000821:virology; D011237:Predictive Value of Tests; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000821:virology; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data