title: Shotgun identification of proteins from uredospores of the bean rust Uromyces appendiculatus.
abstract: We are interested in learning more about the proteome of Uromyces appendiculatus, the fungus that causes common bean rust. Knowledge of the proteins that differentiate life-cycle stages and distinguish infectious bodies such as uredospores, germlings, appressoria, and haustoria may be used to define host-pathogen interactions or serve as targets for chemical inhibition of the fungus. We have used 2-D nanoflowLC-MS/MS to identify more than 400 proteins from asexual uredospores. A majority of the proteins appear to have roles in protein folding or protein catabolism. We present a model by which an abundance of heat shock proteins and translation elongation factors may enhance a spore's ability to survive environmental stresses and rapidly initiate protein production upon germination.
authors.affiliation: Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. cooperb@ba.ars.usda.gov
authors.forename: Bret
authors.initials: B
authors.lastname: Cooper
authors.forename: Wesley M
authors.initials: WM
authors.lastname: Garrett
authors.forename: Kimberly B
authors.initials: KB
authors.lastname: Campbell
mesh_terms: D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000378:metabolism*; D002853:Chromatography, Liquid; D007887:Fabaceae / Q000382:microbiology; D005656:Fungal Proteins / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D017510:Protein Folding; D040901:Proteomics / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D013172:Spores, Fungal / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D010455:Peptides