title: The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription.
abstract: Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein-protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.
authors.affiliation: Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, Box 285, Stockholm 171 77, Sweden. piergiorgio.percipalle@ki.se
authors.forename: Piergiorgio
authors.initials: P
authors.lastname: Percipalle
authors.forename: Nathalie
authors.initials: N
authors.lastname: Fomproix
authors.forename: Erica
authors.initials: E
authors.lastname: Cavellán
authors.forename: Renate
authors.initials: R
authors.lastname: Voit
authors.forename: Georg
authors.initials: G
authors.lastname: Reimer
authors.forename: Tim
authors.initials: T
authors.lastname: Krüger
authors.forename: Johan
authors.initials: J
authors.lastname: Thyberg
authors.forename: Ulrich
authors.initials: U
authors.lastname: Scheer
authors.forename: Ingrid
authors.initials: I
authors.lastname: Grummt
authors.forename: Ann-Kristin Ostlund
authors.initials: AK
authors.lastname: Farrants
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D024461:Myosin Type I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011485:Protein Binding / Q000235:genetics; D012318:RNA Polymerase I / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D000092468:Bromodomain Containing Proteins
chemical_list: D000251:Adenosine Triphosphatases; D002868:Chromosomal Proteins, Non-Histone; D047908:Intracellular Signaling Peptides and Proteins; D024461:Myosin Type I; D009687:Nuclear Proteins; D012318:RNA Polymerase I; D014157:Transcription Factors; C116750:BAZ1B protein, human; D000092468:Bromodomain Containing Proteins; C099927:NMI protein, human; C409462:SMARCA5 protein, human; C460367:MYO1C protein, human