Expression of stem cell markers on mononuclear cells derived from heparinized cadaveric organ donors before and after disconnection from the respirator.
We have attempted to evaluate the level of the earliest human hematopoietic cell marker expression (CD34, CD117, CD133, CD184) on cells obtained from heparinized cadaveric organ donors before and after disconnection from the respirator. Moreover, we compared various cell populations: (1) coexpressing CD34/CD117; (2) CD34/CD133; (3) highly enriched hematopoietic stem cells (CD34+CXCR4+CD45+); and (4) highly enriched tissue-committed stem cells (CD34+CXCR4+CD45-). Finally, we analyzed whether the level of hematopoietic stem cell marker expression depended on the age of the donor. The expression of the membrane receptors (CD34, CD45, CD117, CD133, CD184) was studied by flow cytometry. We observed that the proportion of mononuclear cells expressing these markers slightly decreased in bone marrow harvested after disconnection from the respirator compared with the samples obtained before disconnection. Moreover, the proportion of cells expressing CD117 antigen depended on age of the donor.
Machaliński
B
B
Department of General Pathology, Pomeranian Medical University, Al. Powstańców Wlkp. 72, 70-111 Szczecin, Poland. machalin@sci.pam.szczecin.pl
Paczkowska
E
E
Hałasa
M
M
Pabisiak
K
K
Walczak
M
M
Sieńko
J
J
Kozik
W
W
Ostrowski
M
M
Syrenicz
A
A
Sulikowski
T
T
Machalińska
A
A
D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000502:physiology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting / Q000379:methods; D012122:Ventilators, Mechanical
D015703:Antigens, CD; D015415:Biomarkers