title: Pseudonocardia ammonioxydans sp. nov., isolated from coastal sediment.
abstract: Actinomycete strain H9T was isolated from coastal sediment of the Jiao-Dong peninsula (near Tsingdao city) in China, and was identified by means of polyphasic taxonomy. The strain grew autotrophically on modified nitrifying medium and heterotrophically on Luria-Bertani medium, with NaCl ranging from 0 to 8% (w/v) (optimal growth at 3.5%). The 16S rRNA gene sequence similarities of strain H9T to members of the genus Pseudonocardia ranged from 93.0 to 97.5%, indicating that strain H9T was phylogenetically related to members of the genus Pseudonocardia. Strain H9T had type IV cell wall and type PIII phospholipid, and its major menaquinone was MK-8 (H4). DNA-DNA relatedness values between strain H9T and Pseudonocardia kongjuensis, Pseudonocardia autotrophica and Pseudonocardia compacta were 42, 13 and 11%, respectively. These results support the conclusion that H9T represents a novel species within the genus Pseudonocardia, for which the name Pseudonocardia ammonioxydans sp. nov. is proposed, with the type strain H9T (= CGMCC 4.1877T = JCM 12462T).
authors.affiliation: State Key Laboratory of Microbial Resources at the Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, PR China.
authors.forename: Zhi-Pei
authors.initials: ZP
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources at the Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, PR China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Microbial Resources at the Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, PR China.
authors.forename: Zhi-Heng
authors.initials: ZH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources at the Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, PR China.
authors.forename: Shuang-Jiang
authors.initials: SJ
authors.lastname: Liu
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D001482:Base Composition; D002681:China; D004275:DNA, Ribosomal / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S