Cp*Rh-based indicator-displacement assays for the identification of amino sugars and aminoglycosides.
Indicator-displacement assays based on the organometallic complex [{Cp*RhCl2}2] (Cp*=pentamethylcyclopentadienyl) and the dye gallocyanine were used to sense amino sugars and aminoglycosides in buffered aqueous solution by conducting UV-visible spectroscopy. The data of three assays at pH 7.0, 8.0, and 9.0 were sufficient to distinguish between the amino sugars galactosamine, glucosamine, mannosamine and the aminoglycosides kanamycin A, kanamycin B, amikacin, apramycin, paromomycin, and streptomycin. Furthermore, the assays were used to characterize mixtures of aminoglycosides and obtain quantitative information about the respective analytes.
Zaubitzer
Friederike
F
Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Buryak
Andrey
A
Severin
Kay
K
D000606:Amino Sugars / Q000032:analysis*; D000617:Aminoglycosides / Q000032:analysis*; D002240:Carbohydrate Sequence; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D008969:Molecular Sequence Data; D009942:Organometallic Compounds / Q000737:chemistry*; D010078:Oxazines / Q000737:chemistry; D012238:Rhodium / Q000737:chemistry; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000606:Amino Sugars; D000617:Aminoglycosides; D009942:Organometallic Compounds; D010078:Oxazines; C007062:gallocyanine; D012238:Rhodium