[Changes in renal cell apoptosis and inflammatory cytokines in early stage of sepsis in rats].
OBJECTIVE
To investigate the relationship between changes in renal cell apoptosis and inflammatory cytokines in sepsis rats.


METHODS
Sepsis was produced in rats by cecal ligation and puncture. Changes in tumor necrosis factor-alpha (TNF-alpha) and monocyte chemotactic protein-1 (MCP-1) levels in renal tissues were determined by enzyme-linked immunoadsorbent assay (ELISA), respectively. Meanwhile, apoptosis rate was also assessed by Annexin V/PI staining and flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) methods.


RESULTS
TNF-alpha and MCP-1 levels in renal tissues were significantly increased in rats with sepsis in the early stage. Apoptosis rate of renal cells increased and reached the highest level at 3 hours after sepsis was established and decreased after 6 hours, and the differences were significant (P<0.05 or P<0.01).


CONCLUSIONS
TNF-alpha and MCP-1 are cytokines which are activated in early stage of sepsis. The increase in renal cell apoptosis in the early stage of sepsis is related with the release of cytokines.
Zhang
Yu
Y
Department of Emergency, First Hospital, Dalian Medical College, Dalian 116011, Liaoning, China.
Ren
Yan-bo
YB
Jiang
Li
L
D000818:Animals; D017209:Apoptosis; D018932:Chemokine CCL2 / Q000378:metabolism*; D004195:Disease Models, Animal; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000378:metabolism / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D018932:Chemokine CCL2; D014409:Tumor Necrosis Factor-alpha