title: Experimental axonopathy induced by immunization with Campylobacter jejuni lipopolysaccharide from a patient with Guillain-Barré syndrome.
abstract: New Zealand white rabbits were immunized with a lipopolysaccharide (LPS) extracted from a Campylobacter jejuni HS:19 strain isolated from a GBS patient expressing GM1 and GD1a-like epitopes, Freund's adjuvant (group I) and Freund's adjuvant plus keyhole lympet hemocyanin (KLH) (group II). Both groups showed high titers of anti-LPS and anti-GM1 and lower titers of anti-GD1b and anti-GD1a antibodies. Weakness and axonal degeneration in sciatic nerves was detected in 1/11 of group I and 6/7 of group II. This model replicates, at least in part, the pathogenetic process hypothesized in the human axonal GBS with antiganglioside antibodies post C. jejuni infection and indicates that KLH plays an additional role in neuropathy induction.
authors.affiliation: Department of Oncology and Neurosciences University "G. d'Annunzio" and Istitute of Aging, Ce.S.I., Foundation University "G. d'Annunzio", Chieti-Pescara, Italy.
authors.forename: Christina M
authors.initials: CM
authors.lastname: Caporale
authors.forename: Margherita
authors.initials: M
authors.lastname: Capasso
authors.forename: Mirella
authors.initials: M
authors.lastname: Luciani
authors.forename: Vincenza
authors.initials: V
authors.lastname: Prencipe
authors.forename: Beatrice
authors.initials: B
authors.lastname: Creati
authors.forename: Paola
authors.initials: P
authors.lastname: Gandolfi
authors.forename: Maria V
authors.initials: MV
authors.lastname: De Angelis
authors.forename: Antonio
authors.initials: A
authors.lastname: Di Muzio
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Caporale
authors.forename: Antonino
authors.initials: A
authors.lastname: Uncini
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D015153:Blotting, Western / Q000379:methods; D016123:Campylobacter jejuni / Q000737:chemistry* / Q000276:immunology; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes; D005677:G(M1) Ganglioside / Q000276:immunology; D005732:Gangliosides / Q000276:immunology; D015870:Gene Expression / Q000276:immunology; D020275:Guillain-Barre Syndrome / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D007114:Immunization; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D011129:Polyradiculoneuropathy / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D011817:Rabbits; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial; D000939:Epitopes; D005732:Gangliosides; D008070:Lipopolysaccharides; C021298:ganglioside, GD1a; D005677:G(M1) Ganglioside