title: Mutant p53 protein in serum could be used as a molecular marker in human breast cancer.
abstract: p53 wild-type is a tumor suppressor gene involved in DNA gene transcription or DNA repair mechanisms. When damage to DNA is unrepairable, p53 induces programmed cell death (apoptosis). The mutant p53 gene is the most frequent molecular alteration in human cancer, including breast cancer. Here, we analyzed the genetic alterations in p53 oncogene expression in 55 patients with breast cancer at different stages and in 8 normal women. We measured by ELISA assay the serum levels of p53 mutant protein and p53 antibodies. Immunohistochemistry and RT-PCR using specific p53 primers as well as mutation detection by DNA sequencing were also evaluated in breast tumor tissue. Serological p53 antibody analysis detected 0/8 (0%), 0/4 (0%) and 9/55 (16.36%) positive cases in normal women, in patients with benign breast disease and in breast carcinoma, respectively. We found positive p53 mutant in the sera of 0/8 (0.0%) normal women, 0/4 (0%) with benign breast disease and 29/55 (52.72%) with breast carcinoma. Immunohistochemistry evaluation was positive in 29/55 (52.73%) with mammary carcinoma and 0/4 (0%) with benign breast disease. A very good correlation between p53 mutant protein detected in serum and p53 accumulation by immunohistochemistry (83.3% positive in both assays) was found in this study. These data suggest that detection of mutated p53 could be a useful serological marker for diagnostic purposes.
authors.affiliation: Instituto de Analistas Clinicos Asociados, IACA, Buenos Aires, Argentina. ga_balogh@fccc.edu
authors.forename: G A
authors.initials: GA
authors.lastname: Balogh
authors.forename: D A
authors.initials: DA
authors.lastname: Mailo
authors.forename: M M
authors.initials: MM
authors.lastname: Corte
authors.forename: P
authors.initials: P
authors.lastname: Roncoroni
authors.forename: H
authors.initials: H
authors.lastname: Nardi
authors.forename: E
authors.initials: E
authors.lastname: Vincent
authors.forename: D
authors.initials: D
authors.lastname: Martinez
authors.forename: M E
authors.initials: ME
authors.lastname: Cafasso
authors.forename: A
authors.initials: A
authors.lastname: Frizza
authors.forename: G
authors.initials: G
authors.lastname: Ponce
authors.forename: E
authors.initials: E
authors.lastname: Vincent
authors.forename: E
authors.initials: E
authors.lastname: Barutta
authors.forename: P
authors.initials: P
authors.lastname: Lizarraga
authors.forename: G
authors.initials: G
authors.lastname: Lizarraga
authors.forename: C
authors.initials: C
authors.lastname: Monti
authors.forename: E
authors.initials: E
authors.lastname: Paolillo
authors.forename: R
authors.initials: R
authors.lastname: Vincent
authors.forename: R
authors.initials: R
authors.lastname: Quatroquio
authors.forename: C
authors.initials: C
authors.lastname: Grimi
authors.forename: H
authors.initials: H
authors.lastname: Maturi
authors.forename: M
authors.initials: M
authors.lastname: Aimale
authors.forename: C
authors.initials: C
authors.lastname: Spinsanti
authors.forename: H
authors.initials: H
authors.lastname: Montero
authors.forename: J
authors.initials: J
authors.lastname: Santiago
authors.forename: L
authors.initials: L
authors.lastname: Shulman
authors.forename: M
authors.initials: M
authors.lastname: Rivadulla
authors.forename: M
authors.initials: M
authors.lastname: Machiavelli
authors.forename: G
authors.initials: G
authors.lastname: Salum
authors.forename: M A
authors.initials: MA
authors.lastname: Cuevas
authors.forename: J
authors.initials: J
authors.lastname: Picolini
authors.forename: A
authors.initials: A
authors.lastname: Gentili
authors.forename: R
authors.initials: R
authors.lastname: Gentili
authors.forename: J
authors.initials: J
authors.lastname: Mordoh
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics* / Q000276:immunology; D001943:Breast Neoplasms / Q000097:blood / Q000235:genetics / Q000473:pathology*; D002278:Carcinoma in Situ / Q000097:blood / Q000235:genetics / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000097:blood / Q000235:genetics / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008875:Middle Aged; D009154:Mutation; D009367:Neoplasm Staging; D016159:Tumor Suppressor Protein p53 / Q000097:blood / Q000235:genetics* / Q000276:immunology
chemical_list: D001323:Autoantibodies; D014408:Biomarkers, Tumor; D016159:Tumor Suppressor Protein p53