European and American subgroup C isolates of avian metapneumovirus belong to different genetic lineages.
The gene encoding the attachment glycoprotein (G) was sequenced in three French isolates of-subgroup C avian metapneumovirus (APV-C) from ducks. With 1771 nt, this gene proved as long as recently published for North-American APV-C isolates from turkeys. The nt sequences of the duck viruses shared 99% identity but proved only 75-83% identical with their North-American counterparts, viruses of both origins encoding 585 amino acid (aa)-long G proteins. Alignments revealed more homogeneity within the European and North-American groups (at least 98 and 79% aa identity, respectively) than between European and North-American viruses (at best 70% a identity), and confirmed the presence of an extracellular divergent domain (positions 302-484) in APV-C G. A phylogenetic analysis demonstrated that North-American and French isolates of APV-C belonged to significantly different genetic lineages, in agreement with the different geographical origin and host species of these viruses.
Toquin
D
D
French Agency for Food Safety (AFSSA), Avian and Rabbit Virology Immunology and parasitology Unit (VIPAC), BP53, 22440, Ploufragan, France.
Guionie
O
O
Jestin
V
V
Zwingelstein
F
F
Allee
C
C
Eterradossi
N
N
D000595:Amino Acid Sequence; D000818:Animals; D004372:Ducks; D005060:Europe; D005814:Genes, Viral; D029121:Metapneumovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009656:North America; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000235:genetics
D014759:Viral Envelope Proteins; C116204:attachment protein G