Application of RNA interference in tick salivary gland research.
Ticks are obligate ectoparasites that feed on a variety of hosts including mammals, birds and reptiles. Prolonged attachment on the host and an ability to transmit a wide variety of pathogens are the special features of tick feeding. Salivary glands are the major route for secretion of excess fluid, several proteins, and factors that counteract the host immune response and hence play a significant role in the success of tick feeding. RNA interference (RNAi) enables scientists to silence genes encoding proteins in an absolutely sequence specific manner at the mRNA level. This technique has already been successfully employed in analyzing roles of proteins of important functions or in assigning roles to several proteins of unknown functions in a variety of animals. In this review, we outline the process of RNAi and the applicability of RNAi in tick salivary gland research.
Ramakrishnan
Vijay G
VG
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, 74078, USA.
Aljamali
Majd N
MN
Sauer
John R
JR
Essenberg
Richard C
RC
D000818:Animals; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012469:Salivary Glands / Q000737:chemistry / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism*; D013987:Ticks / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D019476:Insect Proteins; D012471:Salivary Proteins and Peptides