title: Crystallization and preliminary X-ray characterization of a ferritin from the hyperthermophilic archaeon and anaerobe Pyrococcus furiosus.
abstract: Crystals of the title protein have been produced and preliminary structural analysis has been carried out. The crystals belong to the orthorhombic space group C222(1), with unit-cell parameters a = 258.1, b = 340.1, c = 266.5 A. The protein forms a 24-mer of 20 kDa subunits, which assemble with 432 non-crystallographic symmetry. A total of 36 monomers are found in the asymmetric unit, corresponding to one and a half 24-mers.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. República, EAN, 2784-505 Oeiras, Portugal. matias@itqb.unl.pt
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Matias
authors.forename: Jana
authors.initials: J
authors.lastname: Tatur
authors.forename: Maria Arménia
authors.initials: MA
authors.lastname: Carrondo
authors.forename: Wilfred R
authors.initials: WR
authors.lastname: Hagen
mesh_terms: D001105:Archaea / Q000201:enzymology*; D002478:Cells, Cultured; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005293:Ferritins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry; D020027:Pyrococcus furiosus / Q000201:enzymology* / Q000378:metabolism; D013696:Temperature
chemical_list: D021122:Protein Subunits; D005293:Ferritins; D007501:Iron