title: Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis.
abstract: Phosphatidylinositol mannosyltransferase (PimA) is an essential enzyme for mycobacterial growth that catalyses the first mannosylation step in phosphatidyl-myo-inositol mannoside (PIM) biosynthesis. The enzyme belongs to the large GT4 family of glycosyltransferases, for which no structure is currently available. Recombinant purified PimA from Mycobacterium smegmatis has been crystallized in the presence of GDP and myo-inositol. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 37.2, b = 72.4, c = 138.2 A, and diffract to 2.4 A resolution.
authors.affiliation: Unité de Biochimie Structurale, CNRS URA 2185, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: Marcelo E
authors.initials: ME
authors.lastname: Guerin
authors.forename: Alejandro
authors.initials: A
authors.lastname: Buschiazzo
authors.forename: Jana
authors.initials: J
authors.lastname: Korduláková
authors.forename: Mary
authors.initials: M
authors.lastname: Jackson
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Alzari
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008364:Mannosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D020102:Mycobacterium smegmatis / Q000201:enzymology* / Q000378:metabolism; D010716:Phosphatidylinositols / Q000096:biosynthesis / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D010716:Phosphatidylinositols; D011994:Recombinant Proteins; C034154:phosphatidylinositol mannoside; D008364:Mannosyltransferases