Assessment of the toxicity of mixtures of copper, 9,10-phenanthrenequinone, and phenanthrene to Daphnia magna: evidence for a reactive oxygen mechanism.
Polycyclic aromatic hydrocarbons and their derivatives are ubiquitous environmental contaminants. They are commonly present in complex mixtures with other contaminants, such as metals. The toxicities of phenanthrene (PHE) and 9,10-phenanthrenequinone (PHQ) with or without Cu were determined using Daphnia magna. Copper was the most toxic among the three chemicals tested, followed by PHQ and then PHE, with 48-h median effective concentrations (EC50s) of 0.96, 1.72, and 5.33 microM, respectively. Copper at 0.31 microM, or approximately the 5% effective concentration, decreased the EC50 of PHQ from 1.72 to 0.28 microM. Likewise, PHQ at 1.2 microM, or approximately the 10% effective concentration, significantly lowered the EC50 of Cu from 0.96 to 0.30 microM. This synergistic effect was not observed, however, in mixtures of Cu and PHE based on the response addition model. Assimilation of Cu wasfound to be similar with or without PHQ at increasing external concentrations of Cu, indicating that the increased toxicity of their mixtures is physiologically based. The ability of Cu plus PHQ to generate reactive oxygen species (ROS) was measured as well. Copper alone caused elevated ROS levels at a low concentration (0.63 microM). With PHQ present, however, this elevation in ROS occurred at an even lower Cu level (0.31 microM). Possible attenuation effects of ascorbic acid (vitamin C) on toxicity and ROS production induced by Cu, PHQ, and their mixtures were then examined. Ascorbic acid protected against Cu and Cu-plus-PHQ mixture-mediated toxicity but did not affect PHQ toxicity. Ascorbic acid also lowered ROS levels in the presence of Cu and Cu plus PHQ. We conclude that there exist potential toxic interactions between metals and modified PAHs and that these interactions can involve ROS formation.
Xie
Fangli
F
Department of Biology, University of Waterloo, Ontario N2L 3G1, Canada.
Koziar
Stephen A
SA
Lampi
Mark A
MA
Dixon
D George
DG
Warren
Norwood P
NP
Borgmann
Uwe
U
Huang
Xiao-Dong
XD
Greenberg
Bruce M
BM
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D003300:Copper / Q000633:toxicity*; D003621:Daphnia; D004347:Drug Interactions; D009153:Mutagens / Q000633:toxicity*; D010616:Phenanthrenes / Q000633:toxicity*; D017382:Reactive Oxygen Species; D014873:Water Pollutants / Q000633:toxicity
D000975:Antioxidants; D009153:Mutagens; D010616:Phenanthrenes; D017382:Reactive Oxygen Species; D014873:Water Pollutants; C005399:9,10-phenanthrenequinone; C031181:phenanthrene; D003300:Copper; D001205:Ascorbic Acid