Gene expression profiles of CD34+ cells in myelodysplastic syndromes: involvement of interferon-stimulated genes and correlation to FAB subtype and karyotype.
To gain insight into the poorly understood pathophysiology of the myelodysplastic syndromes (MDSs), we have determined the gene expression profiles of the CD34+ cells of 55 patients with MDS by using a comprehensive array platform. These profiles showed many similarities to reported interferon-gamma-induced gene expression in normal CD34+ cells; indeed the 2 most up-regulated genes, IFIT1 and IFITM1, are interferon-stimulated genes (ISGs). Alterations in the expression of ISGs may play a role in the hematologic features of MDS, such as peripheral blood cytopenias. Up-regulation of IFIT1 is a potential diagnostic marker for MDS. We determined whether distinct gene expression profiles were associated with specific FAB and cytogenetic groups. CD34+ cells from patients with refractory anemia with ringed sideroblasts (RARS) showed a particular gene expression profile characterized by up-regulation of mitochondrial-related genes and, in particular, of those of heme synthesis (eg, ALAS2). CD34+ cells from patients with the del(5q) had a distinct gene expression profile, characterized by down-regulation of genes assigned to 5q, and up-regulation of the histone HIST1 gene cluster at chromosome 6p21 and of genes related to the actin cytoskeleton. This study provides important and new insights into the pathophysiology of MDS.
Pellagatti
Andrea
A
Leukaemia Research Fund (LRF) Molecular Haematology Unit, Nuffield Department of Clinical Laboratory Sciences (NDCLS), John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom.
Cazzola
Mario
M
Giagounidis
Aristoteles A N
AA
Malcovati
Luca
L
Porta
Matteo G Della
MG
Killick
Sally
S
Campbell
Lisa J
LJ
Wang
Li
L
Langford
Cordelia F
CF
Fidler
Carrie
C
Oscier
David
D
Aul
Carlo
C
Wainscoat
James S
JS
Boultwood
Jacqueline
J
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D018952:Antigens, CD34 / Q000235:genetics* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D007391:International Cooperation; D007621:Karyotyping; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D048868:Adaptor Proteins, Signal Transducing; D018952:Antigens, CD34; D007372:Interferons