title: Extracellular matrix protein 1 inhibits the activity of matrix metalloproteinase 9 through high-affinity protein/protein interactions.
abstract: Extracellular matrix protein 1 (ECM1), an approximately 85-kDa glycoprotein with broad tissue distribution, harbors mutations in lipoid proteinosis (LP), a heritable disease characterized by reduplication of basement membranes and hyalinization of dermis, associated with neurologic disorders. The mechanisms leading from ECM1 mutations to LP phenotype are unknown. In this study, we explored ECM1 protein-protein interactions utilizing yeast two-hybrid genetic screen of human placental library, which identified nine interacting proteins, including matrix metalloproteinase 9 (MMP9). The interactions were confirmed by beta-galactosidase assay with isolated clones and by co-immunoprecipitation which narrowed the interacting segment in ECM1 to the C-terminal tandem repeat 2 (amino acids 236-361). This peptide segment also inhibited MMP9 activity in a gelatin-based ELISA assay. We propose that ECM1-mediated reduction in MMP9 proteolytic activity may have relevance to pathogenesis of LP.
authors.affiliation: Department of Dermatology and Cutaneous Biology, Jefferson Medical College, Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Fujimoto
authors.forename: Joseph
authors.initials: J
authors.lastname: Terlizzi
authors.forename: Sirpa
authors.initials: S
authors.lastname: Aho
authors.forename: Raymond
authors.initials: R
authors.lastname: Brittingham
authors.forename: Andrzej
authors.initials: A
authors.lastname: Fertala
authors.forename: Noritaka
authors.initials: N
authors.lastname: Oyama
authors.forename: John A
authors.initials: JA
authors.lastname: McGrath
authors.forename: Jouni
authors.initials: J
authors.lastname: Uitto
mesh_terms: D015374:Biosensing Techniques; D003001:Cloning, Molecular; D018389:Codon, Nonsense; D004251:DNA Transposable Elements; D004789:Enzyme Activation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015723:Gene Library; D005820:Genetic Testing; D006801:Humans; D047468:Immunoprecipitation; D008065:Lipoid Proteinosis of Urbach and Wiethe / Q000209:etiology / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
chemical_list: D018389:Codon, Nonsense; D004251:DNA Transposable Elements; C490607:ECM1 protein, human; D016326:Extracellular Matrix Proteins; D061965:Matrix Metalloproteinase Inhibitors; D020780:Matrix Metalloproteinase 9