title: Cytotoxic diacetylenic spiroketal enol ethers from Plagius flosculosus.
abstract: Three new diacetylenic spiroketal enol ethers named flosculins A (1), B (2), and C (3), along with five known compounds (4-8) of the same structural class, were isolated from the leaves of Plagius flosculosus. The structures were deduced by extensive 1D and 2D NMR spectroscopy and mass spectrometry. All isolated compounds exhibited significant cytotoxic activity against leukemia cells (Jurkat T and HL-60). Compounds 5-8 induced apoptosis in HL-60 cells with corresponding IC(50) values ranging from 4 to 6 microM.
authors.affiliation: Dipartimento Farmaco Chimico Tecnologico, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Casu
authors.forename: Leonardo
authors.initials: L
authors.lastname: Bonsignore
authors.forename: Manuela
authors.initials: M
authors.lastname: Pinna
authors.forename: Mariano
authors.initials: M
authors.lastname: Casu
authors.forename: Costantino
authors.initials: C
authors.lastname: Floris
authors.forename: Jürg
authors.initials: J
authors.lastname: Gertsch
authors.forename: Filippo
authors.initials: F
authors.lastname: Cottiglia
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D004988:Ethers, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007558:Italy; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013141:Spiro Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004988:Ethers, Cyclic; D013141:Spiro Compounds