Estimation of human sperm gene-specific deoxyribonucleic acid damage by real-time polymerase chain reaction analysis.
It has been reported that spermatozoa of infertile men have poorer DNA integrity than that of fertile men. Using analyses based on real-time polymerase chain reaction, we have estimated the relative gene-specific DNA damage in human spermatozoa and have observed that the spermatozoa of infertile men possess measurably more DNA damage than that of fertile men.
San Gabriel
Maria
M
Division of Urology, Department of Surgery, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
Zhang
Xiaoyang
X
Zini
Armand
A
D020087:Alu Elements / Q000235:genetics; D016022:Case-Control Studies; D003199:Computer Systems; D004249:DNA Damage; D005240:Feasibility Studies; D005914:Globins / Q000235:genetics; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D013094:Spermatozoa; D016615:Telomere / Q000235:genetics
D007335:Insulin-Like Growth Factor II; D005914:Globins