title: [Induction of apoptosis and inhibition of proliferation in Hep-2 by survivin gene targeting in vitro].
abstract: OBJECTIVE
  To study induction of apoptosis and inhibition of proliferation in Hep-2 by survivin gene targeting.
  
  
  METHODS
  Antisense survivin RNA expression vector was constructed and then was transfected to human laryngeal carcinoma cell line Hep-2 by lipofectamine. HpEGFP/survivin cells were abstained by using G418. The level of survivin protein before and after transfection was determined by Western blot. Proliferation activity was measured by MTT assay. The experiment of colony formation in soft agar was carried out. Apoptosis was assessed by flow cytometry and acridine orange (AO).
  
  
  RESULTS
  After antisense survivin RNA plasmids were transfected, the level of survivin protein was inhibited in Hep-2. Compared with control, proliferation of HpEGFP/survivin cells transfected with the recombinant of antisense survivin RNA were suppressed significantly. The experiment of colony formation in soft agar showed the ability of colony formation decreased in HpEGFP/survivin cells compared to control (P <0.05). Apoptosis rate increased about 1.81 folds compared with control.
  
  
  CONCLUSIONS
  The recombination antisense survivin RNA plasmid can partly inhibit the level of survivin protein expression in Hep-2. Antisense survivin RNA can induce apoptosis and inhibit the proliferation of Hep-2 by down-regulating the expression of endogenous survivin in vitro.
authors.affiliation: Department of Otorhinolaryngology, the Second Hospital of Jilin University, Changchun, China.
authors.forename: Lianji
authors.initials: L
authors.lastname: Wen
authors.forename: Changqing
authors.initials: C
authors.lastname: Li
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Lifang
authors.initials: L
authors.lastname: Gao
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.forename: Guofang
authors.initials: G
authors.lastname: Guan
authors.forename: Chunshun
authors.initials: C
authors.lastname: Jin
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018390:Gene Targeting; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D007822:Laryngeal Neoplasms / Q000473:pathology; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D010957:Plasmids; D016372:RNA, Antisense / Q000235:genetics; D000077022:Survivin; D014162:Transfection
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D016372:RNA, Antisense; D000077022:Survivin