title: Simultaneous quantification of methamphetamine, cocaine, codeine, and metabolites in skin by positive chemical ionization gas chromatography-mass spectrometry.
abstract: A positive chemical ionization gas chromatography-mass spectrometric method was validated to simultaneously quantify drugs and metabolites in skin collected after controlled administration of methamphetamine, cocaine, and codeine. Calibration curves (2.5-100 ng/skin biopsy) for methamphetamine, amphetamine, cocaine, norcocaine, benzoylecgonine, cocaethylene, norcocaethylene, anhydroecgonine methyl ester, morphine, codeine, and 6-acetylmorphine (5-100 ng/skin biopsy for ecgonine methyl ester and ecgonine ethyl ester) exhibited correlation coefficients >0.999 and concentrations +/-20% of target. Intra- and inter-run precisions were <10%. This procedure should be useful for postmortem analysis; data are included on drug concentrations in skin after controlled drug administration.
authors.affiliation: National Institute of Scientific Investigation, 331-1 Shinwol 7-dong, Yangcheon-Gu, Seoul 158-707, Republic of Korea.
authors.forename: Wonkyung
authors.initials: W
authors.lastname: Yang
authors.forename: Allan J
authors.initials: AJ
authors.lastname: Barnes
authors.forename: Mary G
authors.initials: MG
authors.lastname: Ripple
authors.forename: David R
authors.initials: DR
authors.lastname: Fowler
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Cone
authors.forename: Eric T
authors.initials: ET
authors.lastname: Moolchan
authors.forename: Heesun
authors.initials: H
authors.lastname: Chung
authors.forename: Marilyn A
authors.initials: MA
authors.lastname: Huestis
mesh_terms: D002138:Calibration; D003042:Cocaine / Q000378:metabolism*; D003061:Codeine / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008694:Methamphetamine / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012867:Skin / Q000378:metabolism*
chemical_list: D008694:Methamphetamine; D003042:Cocaine; D003061:Codeine