title: Biochemical and morphological analysis of basement membrane component expression in corneoscleral and cribriform human trabecular meshwork cells.
abstract: OBJECTIVE
  To determine whether cells of the cribriform trabecular meshwork (TM) express basement membrane (BM) components similar to corneoscleral TM cells and to determine whether cribriform cells are connected to the elastic tendon net of the TM.
  
  
  METHODS
  TM cells of the corneoscleral and the cribriform regions were cultured from 10 eyes of 10 donors, aged 20 to 87 years. Cell types were classified by alpha-smooth muscle actin (smA), desmin, and alphaB-crystallin staining. Expression of collagen type IV (ColIV) chains alpha1 to 6; collagen type VIII (ColVIII) alpha1; laminin subunits alpha1 to 5, beta1 to 3, and gamma1 to 3; and nidogen 1 and 2 was tested in both cell types by semiquantitative RT-PCR (sqPCR). Expression of ColIValpha2, ColVIIIalpha1, laminin beta2, and nidogen 1 was quantified by Northern blot analysis. The response to transforming growth factor (TGF)-beta2 treatment was investigated. Serial tangential and sagittal TM sections of 16 eyes from 10 donors (aged 12-90 years) were used for electron- and immunoelectron microscopy.
  
  
  RESULTS
  Both TM cell types expressed ColIV chains alpha1, alpha2, alpha4, alpha5, and alpha6; ColVIII alpha1; laminin subunits alpha3, alpha4, beta1, beta2, beta3, gamma1, and gamma2; and nidogen 1, as determined by Northern blot analysis and sqPCR. ColIV alpha3; laminin subunits alpha1, alpha2, and gamma3; and nidogen 2 were not detectable by PCR. Responses to TGF-beta2 treatment did not differ between cell types. In vivo, all cribriform cells were in contact with ColIV containing BM material and were found to connect to the cribriform elastic network.
  
  
  CONCLUSIONS
  Cribriform and corneoscleral TM cells show no differences in expression of BM components and response to TGF-beta2. The direct connection of cribriform cells to the elastic tendon network suggests that they are under mechanical tension. This could explain previous findings of alphaB-crystallin expression in the cribriform region.
authors.affiliation: Department of Human Anatomy, University Regensburg, Regensburg, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Fuchshofer
authors.forename: Ulrich
authors.initials: U
authors.lastname: Welge-Lüssen
authors.forename: Elke
authors.initials: E
authors.lastname: Lütjen-Drecoll
authors.forename: Marco
authors.initials: M
authors.lastname: Birke
mesh_terms: D000199:Actins / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001485:Basement Membrane / Q000378:metabolism* / Q000648:ultrastructure; D015152:Blotting, Northern; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D003315:Cornea / Q000166:cytology / Q000378:metabolism*; D003893:Desmin / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D012313:RNA / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012590:Sclera / Q000166:cytology / Q000378:metabolism*; D014129:Trabecular Meshwork / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053781:Transforming Growth Factor beta2; D038203:alpha-Crystallin B Chain / Q000378:metabolism
chemical_list: D000199:Actins; D003893:Desmin; D016326:Extracellular Matrix Proteins; D007797:Laminin; D008562:Membrane Glycoproteins; C509041:TGFB2 protein, human; D016212:Transforming Growth Factor beta; D053781:Transforming Growth Factor beta2; D038203:alpha-Crystallin B Chain; C029967:nidogen; D012313:RNA; D003094:Collagen