Comparison of K-Cl cotransport expression in high and low K dog erythrocytes.
K-Cl cotransport plays a crucial role in regulatory volume decrease of erythrocytes. K-Cl cotransport activities in dog erythrocytes with an inherited high Na-K pump activity (HK) and normal erythrocytes (LK) were compared. Nitrite (NO(2)) stimulated K-Cl cotransport activity in HK cells around 14-fold at 2.4 mM, and it also increased the Km value of this cotransporter. Real-time PCR and western blot analysis revealed that K-Cl cotransporter 1 was dominant, and that the quantity of K-Cl cotransporter 1 protein was comparable between HK and LK erythrocytes. These results suggest that the difference in cotransport activity was not caused by the amount of K-Cl cotransport protein but by a difference in the regulation system, which is susceptible to oxidant.
Ochiai
Hideharu
H
Research Institute of Biosciences, Azabu University, Sagamihara, Kanagawa, Japan.
Higa
Kazunari
K
Hisamatsu
Shin
S
Fujise
Hiroshi
H
D000818:Animals; D048429:Cell Size; D002712:Chlorides / Q000097:blood*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012413:Rubidium / Q000378:metabolism; D012977:Sodium Nitrite / Q000494:pharmacology; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D000096922:K Cl- Cotransporters
D002712:Chlorides; D010042:Ouabain; D011188:Potassium; D012413:Rubidium; D012977:Sodium Nitrite; D027981:Symporters; D000096922:K Cl- Cotransporters