title: CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells.
abstract: The application of human embryonic stem (hES) cells in regenerative medicine will require rigorous quality control measures to ensure the safety of hES cell-derived grafts. During propagation in vitro, hES cells can acquire cytogenetic abnormalities as well as submicroscopic genetic lesions, such as small amplifications or deletions. Many of the genetic abnormalities that arise in hES cell cultures are also implicated in human cancer development. The causes of genetic instability of hES cells in culture are poorly understood, and commonly used cytogenetic methods for detection of abnormal cells are capable only of low-throughput analysis on small numbers of cells. The identification of biomarkers of genetic instability in hES cells would greatly facilitate the development of culture methods that preserve genomic integrity. Here we show that CD30, a member of the tumor necrosis factor receptor superfamily, is expressed on transformed but not normal hES cells, and that CD30 expression protects hES cells against apoptosis.
authors.affiliation: Monash Institute of Medical Research, Monash University, and the Australian Stem Cell Centre, Bldg. 75 STRIP, Wellington Road, Clayton, Victoria 3800 Australia.
authors.forename: Daniella
authors.initials: D
authors.lastname: Herszfeld
authors.forename: Ernst
authors.initials: E
authors.lastname: Wolvetang
authors.forename: Emma
authors.initials: E
authors.lastname: Langton-Bunker
authors.forename: Tung-Liang
authors.initials: TL
authors.lastname: Chung
authors.forename: Adam A
authors.initials: AA
authors.lastname: Filipczyk
authors.forename: Souheir
authors.initials: S
authors.lastname: Houssami
authors.forename: Pegah
authors.initials: P
authors.lastname: Jamshidi
authors.forename: Karen
authors.initials: K
authors.lastname: Koh
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Laslett
authors.forename: Anna
authors.initials: A
authors.lastname: Michalska
authors.forename: Linh
authors.initials: L
authors.lastname: Nguyen
authors.forename: Benjamin E
authors.initials: BE
authors.lastname: Reubinoff
authors.forename: Irene
authors.initials: I
authors.lastname: Tellis
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Auerbach
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Ording
authors.forename: Leendert H J
authors.initials: LH
authors.lastname: Looijenga
authors.forename: Martin F
authors.initials: MF
authors.lastname: Pera
mesh_terms: D015415:Biomarkers / Q000032:analysis; D018236:Carcinoma, Embryonal / Q000378:metabolism* / Q000473:pathology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D017730:Ki-1 Antigen / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D017730:Ki-1 Antigen