title: Evaluation of extracts and oils of mosquito (Diptera: Culicidae) repellent plants from Sweden and Guinea-Bissau.
abstract: In laboratory tests, ethyl acetate extracts of Hyptis suaveolens Poit. from Guinea-Bissau and Rhododendon tomentosum (Stokes) H. Harmaja (formerly Ledum palustre L.) and Myrica gale L. significantly reduced probing activity of Aedes aegypti (L.). In the field in southern Sweden, extracts of leaves of R. tomentosum, M. gale, and Achillea millefolium L. significantly reduced biting by Aedes mosquitoes. Volatile compounds from M. gale, R. tomentosum, A. millefolium, and H. suaveolens were collected by solid phase microextraction (SPME). Alternatively, compounds in the plants were subjected to extraction by organic solvents of different polarities or by steam distillation and collection by SPME. Compounds collected were identified by gas chromatography-mass spectrometry. Leaves of H. suaveolens contained mainly beta-caryophyllene, bergamotene, and terpinolene. The volatile fraction of an ethyl acetate extract of H. suaveolens was collected by SPME and included beta-caryophyllene, (-) -sabinene, beta-pinene, limonene, alpha-pinene, and bergamotene. The main volatiles detected were alpha-pinene, alpha-phellandrene, myrcene, and limonene from M. gale leaves or inflorescences; pcymene, sabinene, and terpinyl acetate from leaves of R. tomentosum; and (-)-germacrene D, beta-pinene, sabinene, and alpha-pinene from A. millefolium leaves or inflorescences. The selected plant species contained numerous volatiles known to have insecticidal, acaricidal, "pesticidal," and/ or insect repellent properties.
authors.affiliation: Medical Entomology Unit, Department of Systematic Zoology, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18 d, SE-752 36 Uppsala, Sweden.
authors.forename: Thomas G T
authors.initials: TG
authors.lastname: Jaenson
authors.forename: Katinka
authors.initials: K
authors.lastname: Pålsson
authors.forename: Anna-Karin
authors.initials: AK
authors.lastname: Borg-Karlson
mesh_terms: D000085:Acetates / Q000737:chemistry; D031162:Achillea / Q000737:chemistry; D000330:Aedes / Q000187:drug effects*; D000818:Animals; D001681:Biological Assay; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006169:Guinea-Bissau; D006801:Humans; D031331:Hyptis / Q000737:chemistry; D007299:Insect Bites and Stings / Q000517:prevention & control; D007302:Insect Repellents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009032:Mosquito Control; D031641:Myrica / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology*; D029793:Rhododendron / Q000737:chemistry; D013548:Sweden
chemical_list: D000085:Acetates; D007302:Insect Repellents; D010936:Plant Extracts; D010938:Plant Oils; C007650:ethyl acetate