title: The isolation of soyasaponins by fractional precipitation, solid phase extraction, and low pressure liquid chromatography.
abstract: Bioactive soyasaponins are present in soybean (Glycine max). In this study, the isolation of soyasaponins in relatively pure form (>80%) using precipitation, solid phase extraction and reverse phase low pressure liquid chromatography (RP-LPLC) is described. Soy flour soyasaponins were separated from non-saponins by methanol extraction and precipitation with ammonium sulphate. Acetylated group A soyasaponins were isolated first by solid phase extraction followed by RP-LPLC (solvent: ethanol-water). Soyasaponins, from a commercial preparation, were saponified and fractionated into deacetylated group A and group B soyasaponins by solid phase extraction (methanol-water). Partial hydrolysis of group B soyasaponins produced a mixture of soyasaponin III and soyasapogenol B monoglucuronide. RP-LPLC of deacetylated group A soyasaponins separated soyasaponin A1 and A2 (38% methanol); of group B soyasaponins isolated soyasaponin I (50% ethanol); and of the partial hydrolysate separated soyasaponin III from soyasapogenol B monoglucuronide (50% ethanol). This methodology provides soyasaponin fractions that are suitable for biological evaluation.
authors.affiliation: Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, M5S 3E2, Canada.
authors.forename: D M
authors.initials: DM
authors.lastname: Gurfinkel
authors.forename: W F
authors.initials: WF
authors.lastname: Reynolds
authors.forename: A V
authors.initials: AV
authors.lastname: Rao
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D005590:Fractional Precipitation; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D012503:Saponins; C407096:soyasapogenol B; C116604:soyasapogenol A; C056684:soyasaponin I; C072357:soyasaponin III; C106126:soyasaponin II; D009828:Oleanolic Acid