A highly thermostable and alkaline amylase from a Bacillus sp. PN5.
A highly thermostable alkaline amylase producing Bacillus sp. PN5 was isolated from soil, which yielded 65.23 U mL(-1) of amylase in medium containing (%) 0.6 starch, 0.5 peptone and 0.3 yeast extract at 60 degrees C, pH 7.0 after 60 h of incubation. Maximum amylase activity was at pH 10.0 and 90 degrees C. The enzyme retained 80% activity after 1 h at pH 10.0. It exhibited 65% activity at 105 degrees C and had 100% stability in the temperature range between 80 and 100 degrees C for 1 h. In addition, there was 86.36% stability after 1-h incubation with sodium dodecylsulphate. These properties indicated possible use of this amylase in starch saccharification and detergent formulation.
Saxena
Rajendra Kumar
RK
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India. rksmicro@hotmail.com
Dutt
Kakoli
K
Agarwal
Lata
L
Nayyar
Preeti
P
D000681:Amylases / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D013696:Temperature
D000681:Amylases