title: Crystallization and preliminary X-ray diffraction analysis of human growth and differentiation factor 5 (GDF-5).
abstract: Growth and differentiation factor 5 (GDF-5) belongs to the large TGF-beta superfamily of secreted signalling proteins and plays a pivotal role in skeletal development during embryogenesis. The gene for human GDF-5 was cloned, expressed in Escherichia coli and purified to homogeneity. Crystals were obtained that diffracted to 2.2 A resolution. A native data set was acquired, showing that the crystals belong to a trigonal space group, i.e. P3(1)21 or P3(2)21, with unit-cell parameters a = b = 97.1, c = 48.3 A. Initial analysis suggest the presence of only one monomer in the asymmetric unit, resulting in a high solvent content of 72% in the crystal.
authors.affiliation: Lehrstuhl für Physiologische Chemie II, Theodor-Boveri Institut für Biowissenschaften (Biozentrum), Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany. mueller@biozentrum.uni-wuerzburg.de
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Mueller
authors.forename: Maike
authors.initials: M
authors.lastname: Gottermeier
authors.forename: Walter
authors.initials: W
authors.lastname: Sebald
authors.forename: Joachim
authors.initials: J
authors.lastname: Nickel
mesh_terms: D019485:Bone Morphogenetic Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D055428:Growth Differentiation Factor 5; D006801:Humans; D012516:Osteosarcoma; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D016212:Transforming Growth Factor beta / Q000737:chemistry
chemical_list: D019485:Bone Morphogenetic Proteins; C525893:GDF5 protein, human; D055428:Growth Differentiation Factor 5; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta