title: Gene silencing in mosquito salivary glands by RNAi.
abstract: Salivary glands are the ultimate site of development in the insect of mosquito born pathogens such as Plasmodium. Mosquito salivary glands also secrete components involved in anti-haemostatic activities and allergic reactions. We investigated the feasibility of RNAi as a tool for functional analysis of genes expressed in Anopheles gambiae salivary glands. We show that specific gene silencing in salivary glands requires the use of large amounts of dsRNA, condition that differs from those for efficient RNAi in other mosquito tissues. Using this protocol, we demonstrated the role of AgApy, which encodes an apyrase, in the probing behaviour of An. gambiae.
authors.affiliation: Unité de Biologie et Génétique du Paludisme, Institut Pasteur, 28 Rue du Dr Roux 75724 Paris Cedex, France. boisson@pasteur.fr
authors.forename: Bertrand
authors.initials: B
authors.lastname: Boisson
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Jacques
authors.forename: Valérie
authors.initials: V
authors.lastname: Choumet
authors.forename: Estelle
authors.initials: E
authors.lastname: Martin
authors.forename: Jiannong
authors.initials: J
authors.lastname: Xu
authors.forename: Ken
authors.initials: K
authors.lastname: Vernick
authors.forename: Catherine
authors.initials: C
authors.lastname: Bourgouin
mesh_terms: D000818:Animals; D000852:Anopheles / Q000033:anatomy & histology / Q000235:genetics*; D001081:Apyrase / Q000235:genetics / Q000378:metabolism; D008297:Male; D010974:Platelet Aggregation; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism*
chemical_list: D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D001081:Apyrase