[Effects of genistein on the expressions of cell cycle proteins in salivary adenoid cystic carcinoma cell line SACC-83].
OBJECTIVE
To investigate the molecular mechanism of cell cycle arrest induced by tyrosine protein kinase inhibitor, genistein, in human salivary adenoid cystic carcinoma cell line SACC-83.


METHODS
SACC-83 cells cultured in vitro were treated with genistein, the expressions of CyclinB1, Cdk1, CyclinD1 and Cdk4 proteins were detected with Western blotting, and the results were quantitatively analyzed by FluorChem V2.0 software, statistical analysis was performed with analysis of variance using SPSS11.5 software.


RESULTS
With the increase of concentration of genistein and the elongation of time, the expression of CyclinB1, Cdk1, CyclinD1 and Cdk4 proteins was significantly decreased. Treated with 220 micromol/L of genistein for 3 days, the expression level of CyclinB1, Cdk1, CyclinD1 and Cdk4 proteins was 58%, 64%, 46% and 43% of the control group, respectively (P<0.01).


CONCLUSIONS
The cell cycle G2/M arrest induced by genistein in human salivary adenoid cystic carcinoma cell line SACC-83 may be associated with the downregulations of CyclinB1, Cdk1, CyclinD1 and Cdk4 protein expressions.
Ma
Jie
J
Department of Oral Medicine, Second Hospital of China Medical University, Shenyang 110004, Liaoning Province, China. zgbl_2003@163.com
Wang
Jie
J
Zhong
Ming
M
Wang
Zhao-yuan
ZY
D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism*; D059447:Cell Cycle Checkpoints; D018797:Cell Cycle Proteins / Q000187:drug effects*; D045744:Cell Line, Tumor; D051358:Cyclin-Dependent Kinase 4 / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D012468:Salivary Gland Neoplasms / Q000378:metabolism*
D018797:Cell Cycle Proteins; D019833:Genistein; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4