title: Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China.
abstract: Three Gram-positive, moderately halophilic, spore-forming rods, designated XH-63T, XH-62 and EJ-15, were isolated from two salt lakes located near Xilin Hot and Ejinor, in Inner Mongolia, China. The strains were strictly aerobic and motile, with spherical, terminal and deforming endospores. They grew at pH 5.0-9.0 (optimal growth at pH 7.5), between 4 and 45 degrees C (optimal growth at 37 degrees C) and at salinities of 1-20% (w/v) total salts, growing optimally at 10% (w/v) salts. They had meso-diaminopimelic acid in the cell wall peptidoglycan and DNA G + C contents of 36.1-37.1 mol%. The polar lipid pattern of strain XH-63T, selected as the representative strain, consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and a phospholipid and two amino phospholipids of unknown structure. This strain possessed anteiso-C(15:0) and anteiso-C(17:0) as the major fatty acids (altogether representing 72.5% of total) and MK-7 as the major menaquinone. 16S rRNA gene analysis of the three strains showed that they were within the Gracilibacillus cluster, with highest sequence similarity (95.4-95.8%) with Gracilibacillus dipsosauri. Based on a combination of phenotypic, chemotaxonomic and phylogenetic features, it is proposed that the three isolates represent a novel species of the genus Gracilibacillus, Gracilibacillus orientalis sp. nov. The type strain is strain XH-63T (= CCM 7326T = AS 1.4250T = CECT 7097T).
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors.forename: I J
authors.initials: IJ
authors.lastname: Carrasco
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Márquez
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China.
authors.forename: X
authors.initials: X
authors.lastname: Yanfen
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Biotechnology, University of the Western Cape, Bellville 7535, Cape Town, South Africa.
authors.forename: D A
authors.initials: DA
authors.lastname: Cowan
authors.affiliation: Genencor International, Leiden, The Netherlands.
authors.forename: B E
authors.initials: BE
authors.lastname: Jones
authors.affiliation: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, UK.
authors.forename: W D
authors.initials: WD
authors.lastname: Grant
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Ventosa
mesh_terms: D001406:Bacillaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D004275:DNA, Ribosomal / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
chemical_list: D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride