Aging of mesenchymal stem cell in vitro.
BACKGROUND
A hot new topic in medical treatment is the use of mesenchymal stem cells (MSC) in therapy. The low frequency of this subpopulation of stem cells in bone marrow (BM) necessitates their in vitro expansion prior to clinical use. We evaluated the effect of long term culture on the senescence of these cells.


RESULTS
The mean long term culture was 118 days and the mean passage number was 9. The average number of PD decreased from 7.7 to 1.2 in the 10th passage. The mean telomere length decreased from 9.19 Kbp to 8.7 kbp in the 9th passage. Differentiation potential dropped from the 6th passage on. The culture's morphological abnormalities were typical of the Hayflick model of cellular aging.


CONCLUSIONS
We believe that MSC enter senescence almost undetectably from the moment of in vitro culturing. Simultaneously these cells are losing their stem cell characteristics. Therefore, it is much better to consider them for cell and gene therapy early on.
Bonab
Mandana Mohyeddin
MM
Hematology-Oncology & BMT Research Center, Shariati Hospital, Tehran University of Medical Sciences, Iran. mohyeddin@sina.tums.ac.ir
Alimoghaddam
Kamran
K
Talebian
Fatemeh
F
Ghaffari
Syed Hamid
SH
Ghavamzadeh
Ardeshir
A
Nikbin
Behrouz
B
D017667:Adipocytes / Q000166:cytology; D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007700:Kinetics; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008875:Middle Aged; D010011:Osteocytes / Q000166:cytology; D016615:Telomere / Q000378:metabolism; D013997:Time Factors