title: Comparison of induction during development between Xenopus tropicalis and Xenopus laevis.
abstract: Several in vitro systems exist for the induction of animal caps using growth factors such as activin. In this paper, we compared the competence of activin-treated animal cap cells dissected from the late blastulae of Xenopus tropicalis and Xenopus laevis. The resultant tissue explants from both species differentiated into mesodermal and endodermal tissues in a dose-dependent manner. In addition, RT-PCR analysis revealed that organizer and mesoderm markers were expressed in a similar temporal and dose-dependent manner in tissues from both organisms. These results indicate that animal cap cells from Xenopus tropicalis have the same competence in response to activin as those from Xenopus laevis.
authors.affiliation: Department of Life Sciences (Biology), Graduate School of Art and Sciences, University of Tokyo, Tokyo, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Sedohara
authors.forename: Keiko
authors.initials: K
authors.lastname: Suzawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Asashima
mesh_terms: D028341:Activins / Q000494:pharmacology; D000818:Animals; D036703:Blastula / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004627:Embryonic Induction / Q000187:drug effects / Q000502:physiology*; D005260:Female; D008297:Male; D014982:Xenopus laevis
chemical_list: D028341:Activins