Usefulness of eschar PCR for diagnosis of scrub typhus.
We report here on the case of a child who was infected with scrub typhus, and we made the diagnosis according to the serology and by performing PCRs on the child's eschar. The patient was treated with azithromycin, and he did not experience any complications. Performing nested PCR on the eschar might be both a rapid diagnostic test for scrub typhus in the early acute stage and a differential test as to whether or not a scab is a scrub typhus eschar.
Lee
Seung-Hyun
SH
Department of Pediatrics, Seonam University College of Medicine, Gwangju, South Korea.
Kim
Dong-Min
DM
Cho
Young Shin
YS
Yoon
Sung Ho
SH
Shim
Soo Kyoung
SK
D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002648:Child; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D012285:Orientia tsutsugamushi / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012612:Scrub Typhus / Q000175:diagnosis* / Q000382:microbiology; D012867:Skin / Q000382:microbiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D008565:Membrane Proteins; C064828:Sta56 antigen, Rickettsia tsutsugamushi