title: Cleaved amplified polymorphic sequence and amplified fragment length polymorphism markers linked to the fertility restorer gene in chili pepper (Capsicum annuum L.).
abstract: Cytoplasmic male sterility (CMS) in plants, which is due to failure to produce functional pollen, is a maternally inherited trait. Specific nuclear genes that sup-press CMS, termed fertility restorer (Rf) genes, have been identified in several plants. In this study, Rf-linked molecular markers in pepper (Capsicum annuum L.) were detected by bulked segregant analysis of eight amplified fragment length polymorphisms (AFLPs). Only AFRF8 was successfully converted to a cleaved amplified polymorphic sequence (CAPS) marker. This was named AFRF8CAPS and genotype determination using it agreed with that obtained with the original AFRF8. A linkage map with a total size of 54.1 cM was constructed with AFRF8CAPS and the seven AFLP markers using the Kosambi function. The AFRF8CAPS marker was shown to be closest to Rf with a genetic distance of 1.8 cM. These markers will be useful for fast and reliable detection of restorer lines during F(1) hybrid seed production and breeding programs in pepper.
authors.affiliation: Center for Plant Molecular Genetics and Breeding Research, Seoul National University, Seoul 151-921, Korea.
authors.forename: Dong Sun
authors.initials: DS
authors.lastname: Kim
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Kim
authors.forename: Jae Hyoung
authors.initials: JH
authors.lastname: Yoo
authors.forename: Byung-Dong
authors.initials: BD
authors.lastname: Kim
mesh_terms: D002212:Capsicum / Q000235:genetics*; D002874:Chromosome Mapping; D005298:Fertility / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage / Q000235:genetics*; D005819:Genetic Markers; D010641:Phenotype; D051479:Plant Infertility / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*
chemical_list: D005819:Genetic Markers