Graphite epoxy composite electrodes modified with bacterial cells.
The modification of a graphite-epoxy composite electrode (GECE) with bacterial cells along with an analytical application are presented. Pseudomonas putida DSM 50026 was used as a biological component and the measurement was based on the respiratory activity of the cells. The optimization of working conditions of resulting biosensor (including pH and temperature) was conducted and the limit of detection was calculated as 7 microM phenol based on the signal to noise ratio. Then the system was applied for xenobiotic detection. Resulting sample signals were found to be very similar with the standard solutions having the same concentration while the recoveries of the spiked samples were close to 100%.
Kirgöz
Ulkü Anik
UA
Mugla University, Faculty of Arts and Sciences, Chemistry Department, 48000 Kötekli-Mugla.
Odaci
Dilek
D
Timur
Suna
S
Merkoçi
Arben
A
Pazarlioğlu
Nurdan
N
Telefoncu
Azmi
A
Alegret
Salvador
S
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D004852:Epoxy Compounds / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D016958:Pseudomonas putida / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D013696:Temperature; D015262:Xenobiotics / Q000032:analysis
D004852:Epoxy Compounds; D010636:Phenols; D015262:Xenobiotics; D006108:Graphite