Interaction of hemoglobin vesicles, a cellular-type artificial oxygen carrier, with human plasma: effects on coagulation, kallikrein-kinin, and complement systems.
Hemoglobin vesicles (HbVs), cellular-type artificial oxygen carriers containing human hemoglobin, were assessed for their biocompatibility by mixing with human plasma in vitro. Among three kinds of HbVs (PEG-DPEA-HbV, PEG-DPPG-HbV and DPPG-HbV), PEG-DPEA-HbV did not affect the extrinsic or intrinsic coagulation activities of the plasma, while PEG-DPPG-HbV and DPPG-HbV tended to shorten the intrinsic coagulation time. The kallikrein-kinin cascade of the plasma was slightly activated by PEG-DPPG-HbV and DPPG-HbV, but not by PEG-DPEA-HbV. The complement consumption of the plasma was observed by incubation with DPPG-HbV, but not with PEG-DPEA-HbV or PEG-DPPG-HbV. These results indicate that PEG-DPEA-HbV has a higher biocompatibility with human plasma.
Abe
Hideki
H
Hokkaido Red Cross Blood Center, Japanese Red Cross, Yamanote 2-2, Nishi-ku, Sapporo 063-0002, Japan. abeh@hokkaido.bc.jrc.or.jp
Fujihara
Mitsuhiro
M
Azuma
Hiroshi
H
Ikeda
Hisami
H
Ikebuchi
Kenji
K
Takeoka
Shinji
S
Tsuchida
Eishun
E
Harashima
Hideyoshi
H
D001777:Blood Coagulation / Q000187:drug effects*; D001802:Blood Substitutes / Q000737:chemistry / Q000494:pharmacology*; D003165:Complement System Proteins / Q000737:chemistry* / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D007610:Kallikreins / Q000737:chemistry* / Q000378:metabolism; D007705:Kinins / Q000737:chemistry* / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing / Q000379:methods; D010100:Oxygen / Q000737:chemistry; D010949:Plasma / Q000737:chemistry / Q000378:metabolism
D001802:Blood Substitutes; D006454:Hemoglobins; D007705:Kinins; D008081:Liposomes; D003165:Complement System Proteins; D007610:Kallikreins; D010100:Oxygen