title: Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA microarray.
abstract: A central challenge of genomics is to detect, simply and inexpensively, all differences in sequence among the genomes of individual members of a species. We devised a system to detect all single-nucleotide differences between genomes with the use of data from a single hybridization to a whole-genome DNA microarray. This allowed us to detect a variety of spontaneous single-base pair substitutions, insertions, and deletions, and most (>90%) of the approximately 30,000 known single-nucleotide polymorphisms between two Saccharomyces cerevisiae strains. We applied this approach to elucidate the genetic basis of phenotypic variants and to identify the small number of single-base pair changes accumulated during experimental evolution of yeast.
authors.affiliation: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA. dgresham@genomics.princeton.edu
authors.forename: David
authors.initials: D
authors.lastname: Gresham
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Ruderfer
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Pratt
authors.forename: Joseph
authors.initials: J
authors.lastname: Schacherer
authors.forename: Maitreya J
authors.initials: MJ
authors.lastname: Dunham
authors.forename: David
authors.initials: D
authors.lastname: Botstein
authors.forename: Leonid
authors.initials: L
authors.lastname: Kruglyak
mesh_terms: D019020:Directed Molecular Evolution; D005800:Genes, Fungal; D016681:Genome, Fungal; D023281:Genomics; D009154:Mutation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D017384:Sequence Deletion; D013489:Suppression, Genetic