[Transcriptional regulation of placental transforming growth factor-beta by calcitriol in prostate cancer cells is androgen-independent].
Calcitriol (1 alpha,25-dihydroxycholecalciferol) inhibits prostate cancer cell growth. It has been shown that inhibition of growth of prostate cancer LNCaP cells by calcitriol is androgen-dependent. Using cDNA microarray we showed that calcitriol induced expression of placental transforming growth factor-beta (PTGF-beta), which is known to suppress cell growth. We studied regulation of PTGF-beta gene expression by calcitriol and 5alpha-dihydrotestosterone and analyzed whether induction of PTGF-beta transcription by calcitriol is androgen-dependent. Using real-time PCR we demonstrate that 5alpha-dihydrotestosterone up-regulates PTGF-beta mRNA. We do not find an effect of 5alpha-dihydrotestosterone or antiandrogen Casodex on calcitriol-induced PTGF-beta mRNA level and conclude that induction of PTGF-beta transcription by calcitriol is androgen-independent.
Nazarova
N Iu
NIu
Chikhirzhina
G I
GI
Tuohimaaa
P
P
D043582:5-alpha-Dihydroprogesterone / Q000494:pharmacology; D000726:Androgen Antagonists / Q000494:pharmacology; D000728:Androgens / Q000502:physiology*; D000813:Anilides / Q000494:pharmacology; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis*; D002117:Calcitriol / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D055436:Growth Differentiation Factor 15; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D009570:Nitriles; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D014105:Tosyl Compounds
D000726:Androgen Antagonists; D000728:Androgens; D000813:Anilides; D019485:Bone Morphogenetic Proteins; C526140:GDF15 protein, human; D055436:Growth Differentiation Factor 15; D008565:Membrane Proteins; D009570:Nitriles; D014105:Tosyl Compounds; D043582:5-alpha-Dihydroprogesterone; C053541:bicalutamide; D002117:Calcitriol