Methylocystis rosea sp. nov., a novel methanotrophic bacterium from Arctic wetland soil, Svalbard, Norway (78 degrees N).
A Gram-negative, rod-shaped, non-motile, non-spore-forming, pink-pigmented bacterium, SV97T, was isolated from a wetland soil near Ny-Alesund, Svalbard Islands, Norway (78 degrees N). On the basis of 16S rRNA gene sequence similarity, strain SV97T was shown to belong to the Alphaproteobacteria and was highly related to a number of non-characterized Methylocystis strains with GenBank accession nos AJ458507 and AJ458502 (100 %) and AF177299, AJ458510, AJ458467, AJ458471, AJ431384, AJ458475, AJ458484, AJ458501 and AJ458466 (99 %). The most closely related type strains were Methylocystis parvus OBBP(T) (97.2 %) and Methylocystis echinoides IMET 10491T (97%). The closest related recognized species within the genus Methylosinus was Methylosinus sporium NCIMB 11126T (96.0% similarity). Chemotaxonomic and phenotypic data (C(18:1)omega8 as the major fatty acid, non-motile, no rosette formation) supported the affiliation of strain SV97T to the genus Methylocystis. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain SV97(T) from the two recognized Methylocystis species. Strain SV97T therefore represents a novel species, for which the name Methylocystis rosea sp. nov. is proposed, with the type strain SV97T (= DSM 17261T = ATCC BAA-1196T).
Wartiainen
Ingvild
I
Department of Biology, Faculty of Science, University of Tromsø, N-9037 Tromsø, Norway.
Hestnes
Anne Grethe
AG
Department of Biology, Faculty of Science, University of Tromsø, N-9037 Tromsø, Norway.
McDonald
Ian R
IR
Department of Biological Sciences, University of Waikato, Private Bag 3105, Hamilton, New Zealand.
Svenning
Mette M
MM
Department of Biology, Faculty of Science, University of Tromsø, N-9037 Tromsø, Norway.
D001110:Arctic Regions; D001482:Base Composition; D004275:DNA, Ribosomal / Q000737:chemistry; D042083:Methylocystaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D009664:Norway; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S