Crystallization and X-ray analysis of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics.
A recombinant 2-deoxy-scyllo-inosose synthase from Bacillus circulans has been crystallized at 277 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.30 A resolution at 100 K using synchrotron radiation at the Photon Factory. The crystals are monoclinic and belong to space group P2(1), with unit-cell parameters a = 80.5, b = 70.4, c = 83.0 A, beta = 117.8 degrees. The presence of two molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 2.89 A3 Da(-1) and a solvent constant of 57.4% by volume.
Nango
Eriko
E
Department of Chemistry, Tokyo Institute of Technology, O-okayama, Tokyo 152-8551, Japan.
Kumasaka
Takashi
T
Sato
Takao
T
Tanaka
Nobuo
N
Kakinuma
Katsumi
K
Eguchi
Tadashi
T
D000617:Aminoglycosides / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D006595:Hexosamines / Q000737:chemistry; D008190:Lyases / Q000737:chemistry*; D008956:Models, Chemical; D015233:Models, Statistical; D019761:Phosphorus-Oxygen Lyases / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D017356:Synchrotrons; D013696:Temperature
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D006595:Hexosamines; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; D008190:Lyases; C020001:3-dehydroquinate synthetase; D019761:Phosphorus-Oxygen Lyases; C085631:2-deoxy-scyllo-inosose synthase; C007265:2-deoxystreptamine