Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence.
OBJECTIVE
We have previously shown that phototherapy increases cell growth and impairs protein secretion of fibroblasts. Our objective was to study the effect of phototherapy on osteoblast-like cells in culture treated with dexamethasone.


METHODS
Rat calvaria osteoblast-like cells were previously treated or not with dexamethasone and then, they were irradiated or not with a GaAlAs diode laser (wavelength of 780 nm, 10 mW, 3 J/cm2). Adhesion, proliferation, and osteonectin synthesis were analyzed.


RESULTS
Phototherapy increased the proliferation rate of cells independently of dexamethasone presence. Adhesion and osteonectin synthesis were not significantly influenced by laser and/or dexamethasone.


CONCLUSIONS
Based on the conditions of this study we concluded that phototherapy acts as a proliferative stimulus on osteoblast-like cells, even under the influence of dexamethasone. Thus, we suggest that phototherapy can be of importance as co-adjuvant in bone clinical manipulation in order to accelerate bone regeneration.
Fujihara
Neusa A
NA
Department of Oral Pathology, São Paulo, São Paulo, Brazil 05508-900.
Hiraki
Karen R N
KR
Marques
Márcia M
MM
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000528:radiation effects; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D003907:Dexamethasone / Q000494:pharmacology; D028022:Low-Level Light Therapy; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects*; D015676:Osteonectin / Q000096:biosynthesis; D051381:Rats
D000893:Anti-Inflammatory Agents; D015676:Osteonectin; D003907:Dexamethasone