title: Purification, crystallization and preliminary X-ray diffraction analysis of the histone chaperone cia1 from fission yeast.
abstract: In fission yeast, cia1+ is an essential gene that encodes a histone chaperone, a homologue of human CIA (CCG1-interacting factor A) and budding yeast Asf1p (anti-silencing function-1), which both facilitate nucleosome assembly by interacting with the core histones H3/H4. The conserved domain (residues 1-161) of the cia1+-encoded protein was expressed in Escherichia coli, purified to near-homogeneity and crystallized by the sitting-drop vapour-diffusion method. The protein was crystallized in the monoclinic space group C2, with unit-cell parameters a = 79.16, b = 40.53, c = 69.79 A, beta = 115.93 degrees and one molecule per asymmetric unit. The crystal diffracted to beyond 2.10 A resolution using synchrotron radiation.
authors.affiliation: RIKEN Genomic Sciences Center, 1-7-22 Suehiro, Tsurumi, Yokohama 230-0045, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Umehara
authors.forename: Yumi
authors.initials: Y
authors.lastname: Otta
authors.forename: Keiko
authors.initials: K
authors.lastname: Tsuganezawa
authors.forename: Takehisa
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Akiko
authors.initials: A
authors.lastname: Tanaka
authors.forename: Masami
authors.initials: M
authors.lastname: Horikoshi
authors.forename: Balasundaram
authors.initials: B
authors.lastname: Padmanabhan
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D018797:Cell Cycle Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D020868:Gene Silencing; D005822:Genetic Vectors; D006657:Histones / Q000737:chemistry; D015233:Models, Statistical; D009707:Nucleosomes / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D012568:Schizosaccharomyces; D013696:Temperature; D014158:Transcription, Genetic; D014961:X-Ray Diffraction
chemical_list: D018797:Cell Cycle Proteins; C472823:Cia1p protein, S cerevisiae; D018076:DNA, Complementary; D006657:Histones; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins