title: Characterization of a novel glycine-rich protein from the cell wall of maize silk tissues.
abstract: The isolation, characterization and regulation of expression of a maize silk-specific gene is described. zmgrp5 (Zea mays glycine-rich protein 5) encodes a 187 amino acid glycine-rich protein that displays developmentally regulated silk-specific expression. Northern, Western, in situ mRNA hybridization and transient gene expression analyses indicate that zmgrp5 is expressed in silk hair and in cells of the vascular bundle and pollen tube transmitting tissue elements. The protein is secreted into the extracellular matrix and is localized in the cell wall fraction mainly through interactions mediated by covalent disulphide bridges. Taken together, these results suggest that the protein may play a role in maintaining silk structure during development. This is the first documented isolation of a stigma-specific gene from maize, an important agronomic member of the Poaceae family.
authors.affiliation: Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Bldg., Room 2091, Ottawa, Ontario, Canada.
authors.forename: T Y
authors.initials: TY
authors.lastname: Tao
authors.forename: T
authors.initials: T
authors.lastname: Ouellet
authors.forename: K
authors.initials: K
authors.lastname: Dadej
authors.forename: S S
authors.initials: SS
authors.lastname: Miller
authors.forename: D A
authors.initials: DA
authors.lastname: Johnson
authors.forename: J
authors.initials: J
authors.lastname: Singh
mesh_terms: D000595:Amino Acid Sequence; D002473:Cell Wall / Q000737:chemistry*; D018076:DNA, Complementary / Q000302:isolation & purification; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050436:Regulatory Elements, Transcriptional; D016415:Sequence Alignment; D014158:Transcription, Genetic; D003313:Zea mays / Q000166:cytology*
chemical_list: D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C073976:GRP protein, Zea mays