Mesenchymal lineage precursor cells induce vascular network formation in ischemic myocardium.
Mesenchymal lineage precursors can be reproducibly isolated from adult mammalian bone marrow and grown in culture. Immunoselection with monoclonal antibodies against STRO-1 and vascular-cell-adhesion molecule 1 (VCAM1/CD106) prior to expansion results in a 1,000-fold enrichment of mesenchymal precursors compared to standard isolation techniques. Intramyocardial injection of human STRO-1-selected precursors in an athymic rat model of acute myocardial infarction results in induction of vascular network formation and arteriogenesis coupled with global functional cardiac recovery.
Martens
Timothy P
TP
Division of Cardiothoracic Surgery, Columbia University, New York, NY, USA. tpm2102@columbia.edu
See
Fiona
F
Schuster
Michael D
MD
Sondermeijer
Hugo P
HP
Hefti
Marco M
MM
Zannettino
Andrew
A
Gronthos
Stan
S
Seki
Tetsunori
T
Itescu
Silviu
S
D036541:ADP-ribosyl Cyclase / Q000032:analysis; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D001854:Bone Marrow Cells / Q000276:immunology; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D003326:Coronary Circulation; D004195:Disease Models, Animal; D058851:GPI-Linked Proteins; D006439:Hemodynamics; D006801:Humans; D018189:Immunomagnetic Separation; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D017202:Myocardial Ischemia / Q000503:physiopathology / Q000628:therapy*; D018919:Neovascularization, Physiologic; D051381:Rats; D011923:Rats, Nude; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis
D015703:Antigens, CD; D058851:GPI-Linked Proteins; D019010:Vascular Cell Adhesion Molecule-1; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2