Effect of organogenesis on steroidal saponin biosynthesis in calli cultures of Ruscus aculeatus.
Calli cultures derived from Ruscus aculeatus rhizomes were investigated for their potential to biosynthesize saponins. The capacity of undifferentiated tissues to form steroidal saponins is very limited, but when the calli developed organogenesis, mainly aerial shoots and roots, the saponin production increased significantly. Plantlets regenerated from aerial shoots of Ruscus calli showed a saponin pattern similar to that of the callus cultures but the levels of saponins found in the aerial part and roots were significantly greater.
Palazón
Javier
J
Laboratorio de Fisiología Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avenida Diagonal 643, 08028 Barcelona, Spain. javierpalazon@ub.edu
Moyano
Elisabeth
E
Bonfill
Mercedes
M
Osuna
Lidia T
LT
Cusidó
Rosa M
RM
Piñol
M Teresa
MT
D018533:Biomass; D038081:Organogenesis / Q000502:physiology*; D010936:Plant Extracts / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry; D012038:Regeneration; D027343:Rhizome / Q000502:physiology; D031443:Ruscus / Q000737:chemistry* / Q000254:growth & development / Q000502:physiology*; D012503:Saponins / Q000032:analysis / Q000096:biosynthesis*
D010936:Plant Extracts; D012503:Saponins