title: Does two episodes of acute urinary retention lead to additional ischemia-reperfusion injury in rat bladder?
abstract: OBJECTIVE
  To determine whether two episodes of acute urinary retention lead to additional ischemia-reperfusion injury due to decompression of the bladder, or not.
  
  
  METHODS
  Sham, retention and recurrent retention groups consisting of 5, 8 and 8 Wistar Albino male rats were randomized, respectively. After the bladders of rats were emptied with 3F catheter, penile urethras were clamped with aneurism clamp and waited for 30 min after diuresis was forced. At the end of this period, penile clamps were removed and the bladder was again decompressed with 3F catheter and after 30 min removed for examination. In the recurrent retention group, the same process was repeated after an interval of one week. Malonedialdehyde (MDA) levels, indicator of lipid peroxidation and myeloperoxidase (MPO) levels, indicator of leukocyte activation, were examined biochemically in the tissues of the removed bladders.
  
  
  RESULTS
  In the retention and recurrent retention groups, the average increase in bladder MDA and MPO values was higher than the values of sham group (P < 0.05), however, no significant difference was determined between retention and recurrent retention groups (P > 0.05).
  
  
  CONCLUSIONS
  In the bladder tissue, due to acute urinary retention and following decompression process, ischemia-reperfusion injury occurs. Two episodes of acute urinary retention do not lead to additional the ischemia-reperfusion injury that develops in the bladder.
authors.affiliation: Department of Urology, Pamukkale University School of Medicine, Denizli, Turkey. zsinik@pamukkale.edu.tr
authors.forename: Zafer
authors.initials: Z
authors.lastname: Sinik
authors.forename: Gokhan
authors.initials: G
authors.lastname: Guler
authors.forename: Tahir
authors.initials: T
authors.lastname: Turan
authors.forename: Selahattin
authors.initials: S
authors.lastname: Sert
authors.forename: Zafer
authors.initials: Z
authors.lastname: Aybek
authors.forename: Levent
authors.initials: L
authors.lastname: Tuncay
mesh_terms: D000818:Animals; D019299:Decompression, Surgical / Q000009:adverse effects*; D004195:Disease Models, Animal; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012008:Recurrence; D015427:Reperfusion Injury / Q000209:etiology* / Q000378:metabolism; D016055:Urinary Retention / Q000150:complications* / Q000378:metabolism / Q000601:surgery*
chemical_list: D008315:Malondialdehyde; D009195:Peroxidase