Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment.
A rod-shaped bacterium, designated CL-TF13T, was isolated from a tidal flat in Ganghwa, Korea. Analysis of the 16S rRNA gene sequence revealed an affiliation with the genus Tenacibaculum. The sequence similarities between CL-TF13T and type strains of members of the genus Tenacibaculum were from 94.2 to 97.4%. Cells were motile by means of gliding. Strain CL-TF13T grew on solid medium as pale-yellow colonies with an irregular spreading edge. The strain was able to grow in NaCl at a range of 3-5%. They grew within a temperature range of 5-40 degrees C and at pH range of 6-10. The major fatty acids were summed feature 3 (C(16:1)omega7c and/or iso-C(15:0) 2-OH, 19.6%), iso-C(15:0) (18.8%) and iso-C(17:0) 3-OH (13.6%). Fatty acids such as C(18:3)omega6c (6,9,12) (1.5%) and summed feature 4 (iso I- and/or anteiso B-C(17:1), 1.3%) were uniquely found in minor quantities in CL-TF13T among Tenacibaculum species. The DNA G + C content was 30 mol%. According to physiological data, fatty-acid composition and 16S rRNA gene sequence, CL-TF13T could be assigned to the genus Tenacibaculum but distinguished from the recognized species of the genus. Therefore, strain CL-TF13T (= KCCM 42115T = JCM 13039T) represents a novel species, for which the name Tenacibaculum litoreum sp. nov. is proposed.
Choi
Dong Han
DH
School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Kim
Yoon-Gon
YG
School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Hwang
Chung Yeon
CY
School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Yi
Hana
H
School of Biological Sciences, Seoul National University, San 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Chun
Jongsik
J
School of Biological Sciences, Seoul National University, San 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Cho
Byung Cheol
BC
School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
D004275:DNA, Ribosomal / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D007723:Korea; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S