title: Crystallization and preliminary X-ray diffraction analysis of iron regulatory protein 1 in complex with ferritin IRE RNA.
abstract: Iron regulatory protein 1 (IRP1) is a bifunctional protein with activity as an RNA-binding protein or as a cytoplasmic aconitase. Interconversion of IRP1 between these mutually exclusive states is central to cellular iron regulation and is accomplished through iron-responsive assembly and disassembly of a [4Fe-4S] cluster. When in its apo form, IRP1 binds to iron responsive elements (IREs) found in mRNAs encoding proteins of iron storage and transport and either prevents translation or degradation of the bound mRNA. Excess cellular iron stimulates the assembly of a [4Fe-4S] cluster in IRP1, inhibiting its IRE-binding ability and converting it to an aconitase. The three-dimensional structure of IRP1 in its different active forms will provide details of the interconversion process and clarify the selective recognition of mRNA, Fe-S sites and catalytic activity. To this end, the apo form of IRP1 bound to a ferritin IRE was crystallized. Crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 109.6, b = 80.9, c = 142.9 A, beta = 92.0 degrees. Native data sets have been collected from several crystals with resolution extending to 2.8 A and the structure has been solved by molecular replacement.
authors.affiliation: Department of Microbiology and Immunology, University of Illinois, Chicago, Illinois 60612-7334, USA.
authors.forename: Anna I
authors.initials: AI
authors.lastname: Selezneva
authors.forename: Giorgio
authors.initials: G
authors.lastname: Cavigiolio
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Theil
authors.forename: William E
authors.initials: WE
authors.lastname: Walden
authors.forename: Karl
authors.initials: K
authors.lastname: Volz
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D005293:Ferritins / Q000737:chemistry* / Q000235:genetics; D035941:Iron Regulatory Protein 1 / Q000737:chemistry*; D035925:Iron-Regulatory Proteins / Q000737:chemistry* / Q000235:genetics; D011817:Rabbits
chemical_list: D001059:Apoproteins; D035925:Iron-Regulatory Proteins; D005293:Ferritins; D035941:Iron Regulatory Protein 1