title: Isolation of yessotoxin 32-O-[beta-L-arabinofuranosyl-(5'-->1'')-beta-L-arabinofuranoside] from Protoceratium reticulatum.
abstract: Yessotoxin 32-O-[beta-L-arabinofuranosyl-(5'-->1'')-beta-L-arabinofuranoside] (3) was isolated from extracts of Protoceratium reticulatum during a large scale isolation of yessotoxin (1). The structure was characterized by mass spectrometry and NMR spectroscopy. Di-glycoside-3, along with the corresponding mono-glycoside (2) were detected in cultures of P. reticulatum originating from Europe and New Zealand, suggesting that production of arabinosides of 1 is a normal feature of this alga. Formation of multiply charged anions and fragmentation of 3 occurred much more readily than for 1 and 2 under the LC-MS conditions used in this study.
authors.affiliation: Department of Toxinology, AgResearch Ltd, Ruakura Research Centre, PB 3123, East Street, Hamilton, New Zealand. chris.miles@agresearch.co.nz
authors.forename: Christopher O
authors.initials: CO
authors.lastname: Miles
authors.forename: Alistair L
authors.initials: AL
authors.lastname: Wilkins
authors.forename: Andrew I
authors.initials: AI
authors.lastname: Selwood
authors.forename: Allan D
authors.initials: AD
authors.lastname: Hawkes
authors.forename: Dwayne J
authors.initials: DJ
authors.lastname: Jensen
authors.forename: Janine M
authors.initials: JM
authors.lastname: Cooney
authors.forename: Veronica
authors.initials: V
authors.lastname: Beuzenberg
authors.forename: A Lincoln
authors.initials: AL
authors.lastname: MacKenzie
mesh_terms: D000818:Animals; D008958:Models, Molecular; D015394:Molecular Structure; D008978:Mollusk Venoms / Q000737:chemistry* / Q000302:isolation & purification*; D035964:Oxocins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D008978:Mollusk Venoms; D035964:Oxocins; C510944:yessotoxin 32-O-(beta-L-arabinofuranosyl-(5'-1'')-beta-L-arabinofuranoside), Protoceratium reticulatum