title: Enhancement of the binding ability of a ligand for nucleobase recognition by introducing a methyl group.
abstract: The recognition ability of pteridine derivatives for nucleobases opposite an abasic (AP) site in an oligodeoxynucleotide (ODN) duplex is enhanced by using a propylene residue (Spacer-C3) as an AP site. The recognition ability is further enhanced both by attaching methyl groups to a fluorescent ligand and by measuring the fluorescence response at 5 degrees C; 6.2 x 10(6) M(-1) of the binding constant is attained between 2-amino-6,7-dimethyl-4-hydroxypteridine and guanine opposite the AP site in water.
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University, Japan.
authors.forename: Qing
authors.initials: Q
authors.lastname: Dai
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Xu
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Keitaro
authors.initials: K
authors.lastname: Yoshimoto
authors.forename: Seiichi
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Norio
authors.initials: N
authors.lastname: Teramae
mesh_terms: D001665:Binding Sites; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D011485:Protein Binding; D011621:Pteridines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D008024:Ligands; D009705:Nucleosides; D009841:Oligonucleotides; D011621:Pteridines; D014867:Water