Crystallization and halide phasing of the C-terminal domain of human KIN17.
Here, the crystallization and initial phasing of the C-terminal domain of human KIN17, a 45 kDa protein mainly expressed in response to ionizing radiation and overexpressed in certain tumour cell lines, are reported. Crystals diffracting to 1.4 A resolution were obtained from 10% ethylene glycol, 27% PEG 6000, 500 mM LiCl and 100 mM sodium acetate pH 6.3 in space group P2(1)2(1)2(1), with unit-cell parameters a = 45.75, b = 46.31, c = 60.80 A and one molecule in the asymmetric unit. Since this domain has a basic pI, heavy-atom derivatives were obtained by soaking the crystals with negatively charged ions such as tungstate and iodine. The replacement of LiCl by KI in the cryosolution allowed the determination of phases from iodide ions to give an interpretable electron-density map.
le Maire
Albane
A
Département d'Ingénierie et d'Etude des Protéines, Commissariat a l'Energie Atomique, 91191 Gif-sur-Yvette, France. albane.lemaire@cea.fr
Schiltz
Marc
M
Braud
Sandrine
S
Gondry
Muriel
M
Charbonnier
Jean-Baptiste
JB
Zinn-Justin
Sophie
S
Stura
Enrico
E
D002712:Chlorides / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006801:Humans; D007454:Iodides / Q000737:chemistry; D009687:Nuclear Proteins / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D017434:Protein Structure, Tertiary / Q000187:drug effects; D016601:RNA-Binding Proteins; D017973:Tungsten Compounds / Q000737:chemistry
D002712:Chlorides; D004268:DNA-Binding Proteins; D007454:Iodides; C489487:KIN protein, human; D009687:Nuclear Proteins; D010710:Phosphates; D016601:RNA-Binding Proteins; D017973:Tungsten Compounds; C045951:tungstate