Crystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds.
A lectin from Canavalia maritima seeds (ConM) was purified and submitted to crystallization experiments. The best crystals were obtained using the vapour-diffusion method at a constant temperature of 293 K and grew in 7 d. A complete structural data set was collected to 2.1 A resolution using a synchrotron-radiation source. The ConM crystal belongs to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 67.15, b = 70.90, c = 97.37 A. A molecular-replacement search found a solution with a correlation coefficient of 69.2% and an R factor of 42.5%. Crystallographic refinement is under way.
Gadelha
Carlos Alberto de Almeida
CA
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Caixa Postal 6043, CEP 60455-970, Brazil.
Moreno
Frederico Bruno Mendes Batista
FB
Santi-Gadelha
Tatiane
T
Cajazeiras
João Batista
JB
Rocha
Bruno Anderson M da
BA
Rustiguel
Joane Kathelen Rodrigues
JK
Freitas
Beatriz Tupinamba
BT
Canduri
Fernanda
F
Delatorre
Plínio
P
Azevedo
Walter Filgueira de
WF
Cavada
Benildo S
BS
D032449:Canavalia / Q000737:chemistry*; D003460:Crystallization; D037121:Plant Lectins / Q000737:chemistry* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry*; D014961:X-Ray Diffraction
D037121:Plant Lectins; C039268:lectin, Canavalia