Relationship between leukocyte population and nutritive conditions in dairy herds with frequently appearing mastitis.
To clarify the relationship between cellular immune status and nutritive condition, feeding program, blood profiles, and leukocyte populations were analyzed in two dairy herds experiencing frequent mastitis. Fourteen of the 35 lactating cows in herd A, and 18 of the 50 lactating cows in herd B scored positive on the California Mastitis Test (CMT), and 3 of the 73 lactating cows were CMT positive in herd C, which was the control. All herds were evaluated during five different milking stages, and blood was collected from five cows at each stage. With regard to feed content, the percentages of total digestible nutrients (TDN) and crude protein (CP) were found to be lower in herds A and B than in herd C. Levels of serum total cholesterol and blood urea nitrogen were lower in herds A and B than those in herd C. Neutrophil counts in herds A and B were increased compared to the neutrophil counts in herd C. On the other hand, the numbers of CD3(+) T cells and CD14-MHC class(+) cells were lower in herd A and B than in herd C. A decrease in peripheral lymphocytes and undernourishment were observed in the herds with frequent occurring mastitis.
Ohtsuka
Hiromichi
H
Department of Veterinary Internal Medicine, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan.
Kohiruimaki
Masayuki
M
Hayashi
Tomohito
T
Katsuda
Ken
K
Matsuda
Kei-ichi
K
Masui
Machiko
M
Abe
Ryo
R
Kawamura
Sei-ichi
S
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D001806:Blood Urea Nitrogen; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002417:Cattle; D002784:Cholesterol / Q000097:blood; D003612:Dairying; D005260:Female; D006400:Hematocrit / Q000662:veterinary; D007774:Lactation; D007958:Leukocyte Count / Q000662:veterinary; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008414:Mastitis, Bovine / Q000097:blood / Q000276:immunology*; D008892:Milk / Q000382:microbiology; D009752:Nutritional Status / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D001786:Blood Glucose; D001798:Blood Proteins; D017252:CD3 Complex; D018950:Lipopolysaccharide Receptors; D002784:Cholesterol