title: Proteomic comparison of needles from blister rust-resistant and susceptible Pinus strobus seedlings reveals upregulation of putative disease resistance proteins.
abstract: In order to characterize a hypersensitive-like reaction in selected Pinus strobus seedlings to Cronartium ribicola, a proteomic comparison of needles from resistant and susceptible seedlings was undertaken using two-dimensional gel electrophoresis (2-DE). The results revealed 19 polypeptides specific to resistant seedlings and seven of these specific to infected resistant seedlings. There were 13 polypeptides up-regulated (> or = 3-fold increase) in resistant family P327 in comparison to needle tissue from susceptible and mock-inoculated seedlings. Electrospray ionization liquid chromatography and tandem mass spectrometry was used to sequence 11 proteins from the 2-DE gels. Sequences obtained from electrospray ionization liquid chromatography and tandem mass spectrometry were used for MS-BLAST and Pro-ID database searches allowing identification with a 95 to 99% confidence level. Six proteins were determined to be homologs of proteins with known roles in disease resistance, five were determined to be homologs of members of the leucine-rich repeat (LRR) superfamily, and one was a homolog of heat shock protein 90, a protein that serves as a cofactor for certain LRR proteins. This is the first report of members of the LRR family with functional homologs in Pinus strobus and of a molecular basis for white pine blister rust resistance in Pinus strobus.
authors.affiliation: Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Cir., St. Paul, MN 55108, USA. smit1278@umn.edu
authors.forename: Jason A
authors.initials: JA
authors.lastname: Smith
authors.forename: Robert A
authors.initials: RA
authors.lastname: Blanchette
authors.forename: Todd A
authors.initials: TA
authors.lastname: Burnes
authors.forename: James J
authors.initials: JJ
authors.lastname: Jacobs
authors.forename: Leeann
authors.initials: L
authors.lastname: Higgins
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Witthuhn
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: David
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Gillman
mesh_terms: D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000502:physiology; D004198:Disease Susceptibility / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism; D028223:Pinus / Q000033:anatomy & histology / Q000382:microbiology / Q000502:physiology*; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D036226:Seedlings / Q000378:metabolism* / Q000382:microbiology; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D021241:Spectrometry, Mass, Electrospray Ionization; D015854:Up-Regulation
chemical_list: D010455:Peptides; D010940:Plant Proteins; D020543:Proteome