title: Determination of excitatory amino acids in biological fluids by capillary electrophoresis with optical fiber light-emitting diode induced fluorescence detection.
abstract: The capillary electrophoresis (CE) system with optical fiber light-emitting diode (optical fiber LED) induced fluorescence detector was developed for the analysis of the excitatory amino acids (EAAs) tagged with naphthalene-2,3-dicarboxaldehyde (NDA). The separation of EAAs was carried out in an uncoated fused-silica capillary (50 cm x 75 microm i.d.) with a buffer of 10 mM borate at pH 9.3 and an applied voltage of 20 kV. High sensitivity was obtained by the use of optical fiber LED induced fluorescence detector with a violet LED as the excitation light source. The limits of detection (S/N = 3) for glutamic acid (Glu) and aspartic acid (Asp) were 2.1 x 10(-8) and 2.3 x 10(-8) M, respectively. The detection approach was successfully applied to the analysis of Glu and Asp in biological fluids including human serum, rabbit serum and human cerebrospinal fluid (CSF) with satisfactory results.
authors.affiliation: College of Chemistry, Sichuan University, Chengdu 610065, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
authors.forename: Hongyan
authors.initials: H
authors.lastname: Yuan
authors.forename: Dan
authors.initials: D
authors.lastname: Xiao
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D018846:Excitatory Amino Acids / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D006801:Humans; D055095:Optics and Photonics; D011817:Rabbits; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D018846:Excitatory Amino Acids