title: Purification, crystallization and preliminary X-ray analysis of the catalytic domain of the Escherichia coli tRNase colicin D.
abstract: The tRNase domain of colicin D, which cleaves only tRNA(Arg)s at the 3' side of their anticodon loops, has been expressed in Escherichia coli with its inhibitor protein and purified to a form free from the inhibitor using a low-pH buffer. Crystals were obtained by the hanging-drop vapour-diffusion method at 278 K from a buffer containing 100 mM Tris-HCl pH 8.5, 22% PEG MME 2000 and 1 mM nickel(II) chloride. Diffraction data to 1.05 A resolution were collected at BL41XU, SPring-8. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.7, b = 65.5, c = 96.5 A.
authors.affiliation: Department of Biotechnology, The University of Tokyo, Yayoi 1-1-1, Tokyo 113-8657, Japan.
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Takahashi
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Ogawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Hidaka
authors.forename: Kanju
authors.initials: K
authors.lastname: Ohsawa
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Masaki
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yajima
mesh_terms: D020134:Catalytic Domain; D003460:Crystallization; D018360:Crystallography, X-Ray; D004722:Endoribonucleases / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D021381:Protein Transport; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; C501879:colicin D immunity protein, E coli; D004722:Endoribonucleases