title: The gamma-parvin-integrin-linked kinase complex is critically involved in leukocyte-substrate interaction.
abstract: Leukocyte extravasation is an important step of inflammation, in which integrins have been demonstrated to play an essential role by mediating the interaction of leukocytes with the vascular endothelium and the subendothelial extracellular matrix. Previously, we identified an integrin-linked kinase (ILK)-binding protein affixin (beta-parvin), which links initial integrin signals to rapid actin reorganization, and thus plays critical roles in fibroblast migration. In this study, we demonstrate that gamma-parvin, one of three mammalian parvin family members, is specifically expressed in several lymphoid and monocytic cell lines in a complementary manner to affixin. Like affixin, gamma-parvin directly associates with ILK through its CH2 domain and colocalizes with ILK at focal adhesions as well as the leading edge of PMA-stimulated U937 cells plated on fibronectin. The overexpression of the C-terminal fragment containing CH2 domain or the depletion of gamma-parvin by RNA interference inhibits the substrate adhesion of MCP-1-stimulated U937 cells and the spreading of PMA-stimulated U937 cells on fibronectin. Interestingly, the overexpression of the CH2 fragment or the gamma-parvin RNA interference also disrupts the asymmetric distribution of PTEN and F-actin observed at the very early stage of cell spreading, suggesting that the ILK-gamma-parvin complex is essential for the establishment of cell polarity required for leukocyte migration. Taken together with the results that gamma-parvin could form a complex with some important cytoskeletal proteins, such as alphaPIX, alpha-actinin, and paxillin as demonstrated for affixin and actopaxin (alpha-parvin), the results in this study suggest that the ILK-gamma-parvin complex is critically involved in the initial integrin signaling for leukocyte migration.
authors.affiliation: Department of Internal Medicine and Clinical Immunology, Yokohama City Graduate School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan.
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Yoshimi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamaji
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suzuki
authors.forename: Wataru
authors.initials: W
authors.lastname: Mishima
authors.forename: Mayumi
authors.initials: M
authors.lastname: Okamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Obana
authors.forename: Chie
authors.initials: C
authors.lastname: Matsuda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Miwa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ohno
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ishigatsubo
mesh_terms: D000185:Actinin / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D017353:Gene Deletion; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010703:Phorbol Esters / Q000494:pharmacology; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D013379:Substrate Specificity; D000099200:Scaffold Protein ILK
chemical_list: D000185:Actinin; D018932:Chemokine CCL2; D005353:Fibronectins; D020662:Guanine Nucleotide Exchange Factors; D010703:Phorbol Esters; D017346:Protein Serine-Threonine Kinases; D000099200:Scaffold Protein ILK; C486593:PARVG protein, human