Effective proliferation of low level Legionella pneumophila serogroup 1 cells using coculture procedure with Acanthamoeba castellanii.
The multiplications of low level Legionella pneumophila serogroup 1 cells by the coculture procedure with Acanthamoeba castellanii were tested in five strains. The cells in all strains proliferated effectively for isolating. This procedure might be a useful means of improving the successful isolation from environmental and clinical specimens of low level Legionella cells, and pursuing the source of infection.
Seno
Masato
M
Division of 1st Microbiology, Hiroshima Prefectural Institute of Public Health and Environment, Minamimachi 1-6-29, Minami-ku, Hiroshima 734-0007, Japan.
Sakaki
Miyoko
M
Ogawa
Hiromi
H
Matsuda
Hanako
H
Takeda
Yoshihiro
Y
D048248:Acanthamoeba castellanii / Q000254:growth & development*; D000818:Animals; D018920:Coculture Techniques; D015169:Colony Count, Microbial; D016952:Legionella pneumophila / Q000254:growth & development*; D007877:Legionnaires' Disease / Q000517:prevention & control; D014871:Water Microbiology