Cloning, expression, crystallization and preliminary X-ray analysis of a putative multiple antibiotic resistance repressor protein (MarR) from Xanthomonas campestris.
The multiple antibiotic resistance operon (marRAB) is a member of the multidrug-resistance system. When induced, this operon enhances resistance of bacteria to a variety of medically important antibiotics, causing a serious global health problem. MarR is a marR-encoded protein that represses the transcription of the marRAB operon. Through binding with salicylate and certain antibiotics, however, MarR can derepress and activate the marRAB operon. In this report, the cloning, expression, crystallization and preliminary X-ray analysis of XC1739, a putative MarR repressor protein present in the Xanthomonas campestris pv. campestris, a Gram-negative bacterium causing major worldwide disease of cruciferous crops, are described. The XC1739 crystals diffracted to a resolution of at least 1.8 A. They are orthorhombic and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 39.5, b = 54.2 and c = 139.5 A, respectively. They contain two molecules in the asymmetric unit from calculation of the self-rotation function.
Tu
Zhi-Le
ZL
Institute of Biochemistry, National Chung-Hsing University, Taichung 40227, Taiwan.
Li
Juo-Ning
JN
Chin
Ko-Hsin
KH
Chou
Chia-Cheng
CC
Lee
Cheng-Chung
CC
Shr
Hui-Lin
HL
Lyu
Ping-Chiang
PC
Gao
Fei Philip
FP
Wang
Andrew H-J
AH
Chou
Shan-Ho
SH
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics*; D009876:Operon; D011487:Protein Conformation; D012097:Repressor Proteins / Q000737:chemistry; D014961:X-Ray Diffraction; D016959:Xanthomonas campestris / Q000737:chemistry* / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C080004:MarR protein, E coli; D008565:Membrane Proteins; D012097:Repressor Proteins; C075768:secF protein, Bacteria