title: High performance liquid chromatography-mass spectrometry for metabonomics: potential biomarkers for acute deterioration of liver function in chronic hepatitis B.
abstract: Metabonomics methods have been successfully applied to the drug discovery, toxicology, phytochemistry, and clinical fields. Here, we report a self-developed metabonomics platform which is based on high performance liquid chromatography-mass spectrometry (HPLC-MS) technique and applied to the investigation of acute deterioration of liver function in chronic hepatitis B to find the potential biomarkers. Sera from 50 healthy persons and 37 patients with acute deterioration of liver function in chronic hepatitis B were analyzed by HPLC-MS after removal of proteins. After de-noise, peak detection and peak alignment, the data of metabolites were fed to partial least squares discriminant analysis (PLS-DA) to find the potential biomarkers. According to the corresponding tandem mass results, several potential biomarkers were identified: Lysophosphatidyl Choline (LPC) C18:0, LPC C16:0, LPC C18:1, LPC C18:2, and glycochenodeoxycholic acid (GCDCA) (or its isomer glycodeoxycholic acid (GDCA)). On the basis of the relevant literature and pathway databases, the biological significance of the present study is discussed.
authors.affiliation: National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023 Dalian, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Xinjie
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.forename: Chang
authors.initials: C
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Jiangshan
authors.initials: J
authors.lastname: Wang
authors.forename: Lanjuan
authors.initials: L
authors.lastname: Li
authors.forename: Jianren
authors.initials: J
authors.lastname: Gu
authors.forename: Shengli
authors.initials: S
authors.lastname: Yang
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
mesh_terms: D000328:Adult; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005999:Glycochenodeoxycholic Acid; D019694:Hepatitis B, Chronic / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology*; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000503:physiopathology*; D008111:Liver Function Tests; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D005999:Glycochenodeoxycholic Acid