title: Crystallization, data collection and phasing of the molybdate-binding protein of the phytopathogen Xanthomonas axonopodis pv. citri.
abstract: Xanthomonas axonopodis pv. citri ModA protein is the ABC periplasmic binding component responsible for the capture of molybdate. The protein was crystallized with sodium molybdate using the hanging-drop vapour-diffusion method in the presence of PEG or sulfate. X-ray diffraction data were collected to a maximum resolution of 1.7 A using synchrotron radiation. The crystal belongs to the orthorhombic space group C222(1), with unit-cell parameters a = 68.15, b = 172.14, c = 112.04 A. The crystal structure was solved by molecular-replacement methods and structure refinement is in progress.
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: C P
authors.initials: CP
authors.lastname: Santacruz
authors.forename: A
authors.initials: A
authors.lastname: Balan
authors.forename: L C S
authors.initials: LC
authors.lastname: Ferreira
authors.forename: J A R G
authors.initials: JA
authors.lastname: Barbosa
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D008982:Molybdenum / Q000378:metabolism*; D033902:Periplasmic Binding Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D014974:Xanthomonas / Q000737:chemistry*
chemical_list: D018528:ATP-Binding Cassette Transporters; D033902:Periplasmic Binding Proteins; D011994:Recombinant Proteins; C044659:molybdate; D008982:Molybdenum