E-cadherin, laminin and collagen IV expression in the evolution from dysplasia to oral squamous cell carcinoma.
OBJECTIVE
Study the loss or reduction of the cellular adhesion mediated for E-cadherin in oral leukoplakias, oral squamous cell carcinomas and metastatic nodules. Study the loss of continuity of the laminin and collagen IV expression in the epithelial basal membrane from the biological development of the oral leukoplakias and oral carcinomas.


METHODS
we have studied 124 samples of patient pays leukoplakias and oral carcinomas with diverse diagnosis that embrace from normal epithelium (13 samples), mild dysplasias (2), moderate dysplasias (12), in situ carcinomas (13), microinvasive carcinomas (11) oral squamous cell carcinomas (64 samples) and metastatic nodules (9). 7 blocks of tissue microarrays were built with needle of 2mm and was carried out a study by means of immunohistochemical technique for E-cadherin (clone 36, Biogenex), Laminin (078P, Biogenex) and Collagen IV (PHM12, Biogenex).


RESULTS
In Mild and Moderate Dysplasias the results present loss of E-cadherin, Laminin, and Collagen IV (20%) expression. in situ and microinvasive carcinomas, the results presented loss of E-cadherin expression (73%), and loss in Laminin and Collagen IV expression (57%). In the squamous cell carcinomas , we find E-cadherin underexpression (90%) and discontinuity in the Basal Membrane. (70%). All the metastatic nodules presented loss of E-cadherin expression and discontinuity in Laminin and Collagen IV expression.


CONCLUSIONS
The loss of E-cadherin expression is increased when increasing the dysplasia grade of lesions. The loss of continuity in the laminin and Collagen IV expression follow a parallel evolution from dysplasias to metastatic nodules. The underexpression of the three markers has been significant in the evolution of the oral lesions.
Santos-García
Antonio
A
Department of Surgery, University of Salamanca School of Medicine, Salamanca. asantos@infomed.es
Abad-Hernández
M Mar
MM
Fonseca-Sánchez
Emilio
E
Julián-González
Roberto
R
Galindo-Villardón
Purificación
P
Cruz-Hernández
Juan Jesús
JJ
Bullón-Sopelana
Agustín
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015820:Cadherins / Q000096:biosynthesis*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D024141:Collagen Type IV / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007797:Laminin / Q000096:biosynthesis*; D007972:Leukoplakia, Oral / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology*; D011230:Precancerous Conditions
D015820:Cadherins; D024141:Collagen Type IV; D007797:Laminin