Crystallization and preliminary X-ray crystallographic analysis of a galactose-specific lectin from Dolichos lablab.
The galactose-specific lectin from the seeds of Dolichos lablab has been crystallized using the hanging-drop vapour-diffusion technique. The crystals belong to space group P1, with unit-cell parameters a = 73.99, b = 84.13, c = 93.15 A, alpha = 89.92, beta = 76.01, gamma = 76.99 degrees. X-ray diffraction data to a resolution of 3.0 A have been collected under cryoconditions (100 K) using a MAR imaging-plate detector system mounted on a rotating-anode X-ray generator. Molecular-replacement calculations carried out using the available structures of legume lectins as search models revealed that the galactose-specific lectin from D. lablab forms a tetramer similar to soybean agglutinin; two such tetramers are present in the asymmetric unit.
Latha
V Lavanya
VL
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.
Kulkarni
Kiran A
KA
Rao
R Nagender
RN
Kumar
N Siva
NS
Suguna
K
K
D003460:Crystallization; D018360:Crystallography, X-Ray; D031294:Dolichos / Q000737:chemistry* / Q000378:metabolism; D005690:Galactose / Q000737:chemistry / Q000378:metabolism*; D037121:Plant Lectins / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D030262:Soybean Proteins / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry; D010835:Phytohemagglutinins
D005690:Galactose; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins