title: qPCR-DAMS: a database tool to analyze, manage, and store both relative and absolute quantitative real-time PCR data.
abstract: Quantitative real-time PCR is an important high-throughput method in the biomedical sciences. However, existing software has limitations in handling both relative and absolute quantification. We designed quantitative PCR data analysis and management system (qPCR-DAMS), a database tool based on Access 2003, to deal with such shortcomings by the addition of integrated mathematical procedures. qPCR-DAMS allows a user to choose among four methods for data processing within a single software package: 1) ratio relative quantification, 2) absolute level, 3) normalized absolute expression, and 4) ratio absolute quantification. qPCR-DAMS also provides a tool for multiple reference gene normalization. qPCR-DAMS has three quality control steps and a data display system to monitor data variation. In summary, qPCR-DAMS is a handy tool for real-time PCR users.
authors.affiliation: Department of Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA.
authors.forename: Nili
authors.initials: N
authors.lastname: Jin
authors.forename: Keyu
authors.initials: K
authors.lastname: He
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
mesh_terms: D003628:Database Management Systems; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D008962:Models, Theoretical; D016133:Polymerase Chain Reaction