Searching for hemostatic modifier genes affecting the phenotype of mice with very low levels of FVII.
FVII(tTA/-) mice are heterozygous for the FVII- null allele and a gene-targeted allele expressing very low levels of FVII. Approximately half the FVII(tTA/-) in a mixed C57Bl/6:129x1/SVJ background survive much longer than mice in a C57Bl/6 background, suggesting that genetic background affects the survival of mice expressing very low levels of FVII. A lineage of long-term surviving FVII(tTA/-) mice in a predominantly C57Bl/6 background was generated by selecting 16 weeks or older FVII(tTA/-) males and breeding them with C57Bl/6 FVII+/- females. It is postulated that these mice maintain 129x1/SVJ alleles at putative hemostatic modifier loci necessary for the survival of mice expressing very low levels of FVII. A SNP genomic scan was performed on DNA from long-term surviving FVII(tTA/-) mice. The scan identified 8 regions enriched for 129x1/SVJ sequences that might contain putative hemostatic modifier genes.
Rosen
Elliot D
ED
Medical and Molecular Genetics, Indiana University School of Medicine, 975 W Walnut Street, IB130, Indianapolis, IN 46202, USA. edrosen@iupui.edu
Xuei
Xiaoling
X
Suckow
Mark
M
Edenberg
Howard
H
D000818:Animals; D002874:Chromosome Mapping; D005167:Factor VII / Q000032:analysis*; D005168:Factor VII Deficiency / Q000401:mortality; D023281:Genomics; D006706:Homeostasis / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D040641:Quantitative Trait Loci; D017741:Survivors
D005167:Factor VII