title: Purification, crystallization and preliminary X-ray crystallographic analysis of rice lectin from Oryza sativa.
abstract: Lectins with sugar-binding specificity are widely distributed in higher plants and various other species. The expression of rice lectin from Oryza sativa is up-regulated in the growing coleoptile when anaerobic stress persists. A rice lectin of molecular weight 15.2 kDa has been crystallized using the hanging-drop vapour-diffusion method. From the diffraction of the lectin crystals at 1.93 A resolution, the unit cell belongs to space group P3(1), with unit-cell parameters a = 98.58, b = 98.58, c = 44.72 A. Preliminary analysis indicates that there are two lectin molecules in an asymmetric unit with a large solvent content, 70.1%.
authors.affiliation: Life Science Group, Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
authors.forename: Yen-Chieh
authors.initials: YC
authors.lastname: Huang
authors.forename: Yi-Hung
authors.initials: YH
authors.lastname: Lin
authors.forename: Chia-Hao
authors.initials: CH
authors.lastname: Shih
authors.forename: Chun-Liang
authors.initials: CL
authors.lastname: Shih
authors.forename: Tschining
authors.initials: T
authors.lastname: Chang
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Chen
mesh_terms: D000693:Anaerobiosis / Q000502:physiology; D003460:Crystallization; D018360:Crystallography, X-Ray; D012275:Oryza / Q000254:growth & development / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D037121:Plant Lectins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D015854:Up-Regulation
chemical_list: D037121:Plant Lectins; C040599:rice lectin