Target peptide recognition by S100P protein and role of central linker region and dimer interface.
Interaction between S100P and its target protein is an essential step in several cellular functions. The amphipathic mellitin peptide binds tightly to S100P protein in the presence of calcium cation. Since little is known about the recognition sequence, mellitin interaction form a model for S100P. Interaction between mellitin and protein examined to identify key regions required for the protein-protein interaction.
Tutar
Yusuf
Y
Cumhuriyet Universitesi Kimya Bölümü Biyokimya A.B.D. Sivas Turkey. ytutar@cumhuriyet.edu.tr
D002118:Calcium / Q000378:metabolism; D002151:Calorimetry; D002850:Chromatography, Gel; D019281:Dimerization; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008555:Melitten / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D009418:S100 Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
D010455:Peptides; D009418:S100 Proteins; D008555:Melitten; D014443:Tyrosine; D014364:Tryptophan; D002118:Calcium