Naltrexone influences protein kinase Ce and integrin alpha7 activity in SH-SY5Y neuroblastoma cells.
Alcohol influences the neuroadaptation of brain cells where receptors and enzymes like protein kinase C (PKC) exist. Naltrexone acts on opioid receptors. However, other mechanisms of action remain unknown. We prepared SH-SY5Y neuroblastoma cells, and fed them with 150 mM ethanol for 72 h followed by treatment with naltrexone for 24 h. We performed microarray analysis and reverse transcriptase-polymerase chain reaction. Our results showed that PKCepsilon increased 1.90 times and showed an overall decreasing pattern as time increased. Phosphorylated ERK also increased 2.0 times according to the change of PKCepsilon. Integrin alpha7 increased 2.32 times and showed an increasing pattern as time increased. In conclusion, naltrexone influences PKCepsilon neuronal signaling system and endothelial adhesion molecule integrin alpha7 in addition to the well-known opioid system.
Oh
Dong Yul
DY
Department of Psychiatry, Kwandong University, Gangneung 210-701, Korea.
Jung
Kyoung Hwa
KH
Yang
Byung Hwan
BH
Lee
Jun Suk
JS
Choi
Ihn Geun
IG
Chai
Young Gyu
YG
D015703:Antigens, CD / Q000378:metabolism*; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D039001:Integrin alpha Chains / Q000378:metabolism*; D009271:Naltrexone / Q000494:pharmacology*; D009447:Neuroblastoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D051744:Protein Kinase C-epsilon / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D015703:Antigens, CD; D018076:DNA, Complementary; D039001:Integrin alpha Chains; C085725:integrin alpha7; D009271:Naltrexone; D051744:Protein Kinase C-epsilon