Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of XC847, a 3'-5' oligoribonuclease from Xanthomonas campestris.
Oligoribonucleases are essential components of RNA and DNA metabolism and close homologues of genes encoding them are found not only in prokaryotes but also in a wide range of eukaryotes, including yeast and humans. Inactivation of the oligoribonuclease gene (orn) can result in cellular lethality. Despite their important biological function, they have been studied little from a structural point of view. In this report, the cloning, expression, crystallization and preliminary X-ray analysis of XC847, a DEDDh-type 3'-5' oligoribonuclease from the plant pathogen Xanthomonas campestris pv. campestris, a Gram-negative bacterium causing major worldwide disease of cruciferous crops, is described. The XC847 crystals diffracted to a resolution of at least 2.1 A. They are tetragonal and belong to space group P4(3)2(1)2, with unit-cell parameters a = b = 67.5, c = 89.8 A. One molecule is present per asymmetric unit.
Wu
Yan-You
YY
Department of Life Science, National Central University, Jung-Li 300, Taoyuan, Taiwan.
Chin
Ko-Hsin
KH
Chou
Chia-Cheng
CC
Lee
Cheng-Chung
CC
Shr
Hui-Lin
HL
Gao
Fei Philip
FP
Lyu
Ping-Chiang
PC
Wang
Andrew H-J
AH
Chou
Shan-Ho
SH
D020816:Amino Acid Motifs; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005095:Exoribonucleases / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D014961:X-Ray Diffraction; D016959:Xanthomonas campestris / Q000201:enzymology*
D012313:RNA; D004247:DNA; D005095:Exoribonucleases