High-resolution STM imaging of novel poly(G)-poly(C) DNA molecules.
High-resolution scanning tunneling microscopy (STM) imaging of single double-stranded poly(G)-poly(C) DNA molecules, made by a novel synthesis method, shows the molecules morphology. The STM images reveal a periodic structure of approximately 4 nm, seen as repeating "bulbs" along the molecules. These "bulbs" are associated with the molecule helix (the major grooves). "Nicks", two per 100 nm on the average, are observed along the DNA as well. The STM measurements were supported by a morphological statistics of the DNA molecule groove length and apparent height relative to the surface.
Shapir
Errez
E
Physical Chemistry Department, The Hebrew University, Jerusalem 91904, Israel.
Cohen
Hezy
H
Borovok
Natalia
N
Kotlyar
Alexander B
AB
Porath
Danny
D
D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D011066:Poly C / Q000737:chemistry*; D011068:Poly G / Q000737:chemistry*
D011068:Poly G; D011066:Poly C; D004247:DNA