A DNA vaccine encoding a fatty acid-binding protein of Clonorchis sinensis induces protective immune response in Sprague-Dawley rats.
Clonorchis sinensis, the Chinese liver fluke, resides chronically in the biliary tract, and fatty acid-binding protein (FABP) is known to play an important role in the intracellular transport of long-chain fatty acids obtained from the host. Although FABP has stimulated considerable interest as a vaccine candidate, the nature of C. sinensis FABP (CsFABP) remains unclear. We investigated the immunogenicity and protective efficacy of a DNA vaccine encoding CsFABP. The intradermal injection of plasmid DNA carrying the CsFABP gene (pcDNA3.1-FABP) into Sprague-Dawley (SD) rats induced both humoural and cellular immune responses. Animals injected with pcDNA3.1-FABP developed FABP-specific antibody, which is dominance of IgG2a in sera. In addition, the DNA vaccine elicited the production of IFN-gamma, but not the production of IL-4 in spleen cells stimulated with recombinant FABP. Moreover, pcDNA3.1-FABP induced a significant level of protection, decreased worm burden (40.9%, P<0.05) in SD rats against C. sinensis metacerariae challenge. These results suggest that pcDNA3.1-FABP induces a typical T helper-1-dominated immune response and it is a good candidate for use in future clonorchiasis vaccination studies.
Lee
J-S
JS
Department of Biology, Daejeon University, Daejeon, Korea.
Kim
I S
IS
Sohn
W-M
WM
Lee
J
J
Yong
T-S
TS
D000279:Administration, Cutaneous; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D002460:Cell Line; D003003:Clonorchiasis / Q000517:prevention & control*; D003004:Clonorchis sinensis / Q000235:genetics / Q000276:immunology*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D008969:Molecular Sequence Data; D010270:Parasite Egg Count; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology* / Q000627:therapeutic use
D000909:Antibodies, Helminth; D050556:Fatty Acid-Binding Proteins; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D007371:Interferon-gamma