Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features.
Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. In this second article, we investigate the platelet-associated features of this biomaterial. During PRF processing by centrifugation, platelets are activated and their massive degranulation implies a very significant cytokine release. Concentrated platelet-rich plasma platelet cytokines have already been quantified in many technologic configurations. To carry out a comparative study, we therefore undertook to quantify PDGF-BB, TGFbeta-1, and IGF-I within PPP (platelet-poor plasma) supernatant and PRF clot exudate serum. These initial analyses revealed that slow fibrin polymerization during PRF processing leads to the intrinsic incorporation of platelet cytokines and glycanic chains in the fibrin meshes. This result would imply that PRF, unlike the other platelet concentrates, would be able to progressively release cytokines during fibrin matrix remodeling; such a mechanism might explain the clinically observed healing properties of PRF.
Dohan
David M
DM
Biophysics Laboratory, Faculty of Dental Surgery, University of Paris V, Paris, France. drdohand@hotmail.com
Choukroun
Joseph
J
Diss
Antoine
A
Dohan
Steve L
SL
Dohan
Anthony J J
AJ
Mouhyi
Jaafar
J
Gogly
Bruno
B
D000328:Adult; D000077214:Becaplermin; D001792:Blood Platelets / Q000502:physiology*; D002469:Cell Separation / Q000379:methods; D005337:Fibrin / Q000737:chemistry* / Q000494:pharmacology / Q000502:physiology*; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology; D005782:Gels; D006025:Glycosaminoglycans / Q000097:blood; D006133:Growth Substances / Q000097:blood*; D006490:Hemostatics / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000502:physiology; D008297:Male; D044367:Phase Transition; D010974:Platelet Aggregation / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000502:physiology; D020574:Proto-Oncogene Proteins c-sis; D016212:Transforming Growth Factor beta / Q000097:blood; D053773:Transforming Growth Factor beta1; D014945:Wound Healing / Q000187:drug effects*
D015718:Fibrin Tissue Adhesive; D005782:Gels; D006025:Glycosaminoglycans; D006133:Growth Substances; D006490:Hemostatics; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D000077214:Becaplermin; D007334:Insulin-Like Growth Factor I; D005337:Fibrin