Mesna protects intestinal mucosa from ischemia/reperfusion injury.
BACKGROUND
Mesna is a thiol used for the prevention of oxazaphosphorine-induced hemorrhagic cystitis. However, its antioxidant properties on renal and hepatorenal oxidative damage, as well as its mucoprotective effect on the intestinal epithelium have also been shown. The aim of this study was to investigate the potential beneficial effect of mesna on ischemia/reperfusion (I/R)-induced oxidant damage of the intestinal mucosa.


METHODS
Wistar rats were subjected to intestinal I/R for 30 min, induced by occlusion of the superior mesenteric artery, followed by 60 min reperfusion. Mesna was administered at 3 time points relative to ischemia; 60 min before ischemia, at the onset of ischemia or at the onset of reperfusion. At the end of the study period, jejunal segments were excised and assessed for histopathologic score, apoptotic index using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick-end labeling (TUNEL) assay and glutathione/glutathione disulfide (GSH/GSSG) ratio, as a marker of oxidative stress.


RESULTS
I/R caused deterioration of histological characteristics and induction of apoptosis and oxidative stress in the intestinal mucosa. Changes regarding histology and apoptosis were prevented when mesna was administered 60 min before ischemia, but were attenuated when mesna was administered at the onset of ischemia or reperfusion. In all mesna groups, oxidative stress was reduced.


CONCLUSIONS
Mesna can ameliorate or even prevent intestinal I/R injury by reducing oxidative stress.
Ypsilantis
Petros
P
Laboratory of Experimental Surgery, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece. pipsil@med.duth.gr
Lambropoulou
Maria
M
Tentes
Ioannis
I
Kortsaris
Alexandros
A
Papadopoulos
Nikolaos
N
Simopoulos
Constantinos
C
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D017209:Apoptosis; D003250:Constriction; D005978:Glutathione / Q000032:analysis / Q000737:chemistry; D020287:In Situ Nick-End Labeling; D007413:Intestinal Mucosa / Q000473:pathology*; D007583:Jejunum / Q000473:pathology; D017538:Mesenteric Artery, Superior; D015080:Mesna / Q000008:administration & dosage*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*
D000975:Antioxidants; D020011:Protective Agents; D005978:Glutathione; D015080:Mesna