Effect of PEG spacer on cellulose adsorbent for the removal of low density lipoprotein-cholesterol.
Carboxyl modified PEG spacer was synthesized and linked covalently to cellulose beads. L-lysine ligand was coupled to the spacer and its selective affinity for low-density lipoprotein-cholesterol (LDL-C) was determined. It was found that the adsorption capacity and the efficiency of the ligand for adsorption of LDL-C was increased when PEG spacer was used. Experimental results showed that with the chain length of PEG spacers increased from 1000Da to 6000 Da, the average adsorption capacity of LDL-C was enhanced from 0.242 mg/ml to 0.903 mg/ml. Above results indicate that PEG spacers are conducible to the selective removal of LDL-C from human plasma. In addition, LDL-C adsorbent with PEG spacers has a low adsorption capacity for high-density lipoprotein (HDL-C).
Wang
Shenqi
S
Key Laboratory of Bioactive Materials, Ministry of Education Nankai University, Tianjin, P.R. China.
Guo
Xinji
X
Wang
Lianyong
L
Wang
Weichao
W
Yu
Yaoting
Y
D000327:Adsorption; D001781:Blood Component Removal / Q000379:methods; D002482:Cellulose / Q000737:chemistry*; D008078:Cholesterol, LDL / Q000737:chemistry*; D006801:Humans; D008239:Lysine / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
D008078:Cholesterol, LDL; D011092:Polyethylene Glycols; D002482:Cellulose; D008239:Lysine