title: Notch signaling in astrocytes and neuroblasts of the adult subventricular zone in health and after cortical injury.
abstract: The postnatal subventricular zone (SVZ) is a niche for continuous neurogenesis in the adult brain and likely plays a fundamental role in self-repair responses in neurodegenerative conditions. Maintenance of the pool of neural stem cells within this area depends on cell-cell communication such as that provided by the Notch signaling pathway. Notch1 receptor mRNA has been found distributed in different areas of the postnatal brain including the SVZ. Although the identity of Notch1-expressing cells has been established in the majority of these areas, it is still unclear what cell types within the SVZ are expressing components of this pathway. Here we demonstrate that most of expression of Notch1 in the adult SVZ occurs in polysialylated neural cell adhesion molecule (PSA-NCAM)-positive neural precursors and in glial fibrillary acidic protein-positive SVZ astrocytes. Notch1 was also found in PSA-NCAM-positive neuroblasts located within the rostral migratory stream (RMS) but much less in those that have reached the olfactory bulb. We show that two of the naturally occurring Notch1 activators, Jagged1 and Delta1, are also expressed in the SVZ and within the RMS in the adult mouse brain. Finally, using a model of cortical stab wound, we show that the astrogliogenic response of the SVZ to injury is accompanied by activation of the Notch pathway.
authors.affiliation: Laboratory for Gene Therapy of Neurodegenerative Disorders, San Raffaele Telethon Institute for Gene Therapy, Milan, Italy. givogri.maria@hsr.it
authors.forename: Maria I
authors.initials: MI
authors.lastname: Givogri
authors.forename: Maria
authors.initials: M
authors.lastname: de Planell
authors.forename: Francesca
authors.initials: F
authors.lastname: Galbiati
authors.forename: Daniela
authors.initials: D
authors.lastname: Superchi
authors.forename: Angela
authors.initials: A
authors.lastname: Gritti
authors.forename: Angelo
authors.initials: A
authors.lastname: Vescovi
authors.forename: Jean
authors.initials: J
authors.lastname: de Vellis
authors.forename: Ernesto R
authors.initials: ER
authors.lastname: Bongarzone
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D001930:Brain Injuries / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D020547:Lateral Ventricles / Q000166:cytology / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009416:Nerve Regeneration / Q000502:physiology*; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051881:Receptor, Notch1 / Q000235:genetics*; D018121:Receptors, Cytokine / Q000378:metabolism; D000072099:Serrate-Jagged Proteins; D012794:Sialic Acids / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: C498762:CRLF2 protein, human; D002135:Calcium-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D036341:Intercellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; C000606274:Jag1 protein, mouse; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D039842:Neural Cell Adhesion Molecule L1; C497762:Notch1 protein, mouse; D051881:Receptor, Notch1; D018121:Receptors, Cytokine; D000072099:Serrate-Jagged Proteins; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule