[Plant regeneration from callus of Changiums myrnioides].
OBJECTIVE
To set up the callus culture system of Changiums myrnioides and the plantlet regeneration system by the proliferation and differentiation of callus.


METHODS
The segment of stems used as the explants were cultured on dedifferentiation and differentiation medium supplied with different auxin and cytokinin.


RESULTS
MS medium with NAA 0.5 - 1.0 mg/L + KT 0.1 mg/L was best combination for the induction of callus, MS medium with 6 - BA 1 - 2 mg/L + GA 1 - 2 mg/L was suitable for the differentiation of adventitious buds, and MS medium with IBA 0.5 - 1 mg/L was suitable for the induction of root.


CONCLUSIONS
Callus culture may be used as a useful method to get regenerated plantlet of Changiums myrnioides under certain condition.
Xu
Xiuquan
X
College of Biological and Environmental Science, Jiangsu University, Zhenjiang 212013.
Yan
Chunyan
C
Yu
Rongmin
R
D019661:Apiaceae / Q000254:growth & development*; D003470:Culture Media; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D012038:Regeneration; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010937:Plant Growth Regulators