title: Pulsed-field gel electrophoresis as a discriminatory typing technique for the biothreat agent burkholderia mallei.
abstract: Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) was used to type 21 laboratory strains of Burkholderia mallei. We demonstrated good resolution by PFGE together with clustering of some geographically related isolates, and confirmed previous observations that B. mallei is clonal as defined by MLST.
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Narisara
authors.initials: N
authors.lastname: Chantratita
authors.forename: Mongkol
authors.initials: M
authors.lastname: Vesaratchavest
authors.forename: Vanaporn
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: Rachaneeporn
authors.initials: R
authors.lastname: Tiyawisutsri
authors.forename: Tsedev
authors.initials: T
authors.lastname: Ulziitogtokh
authors.forename: Erhan
authors.initials: E
authors.lastname: Akcay
authors.forename: Nicholas P J
authors.initials: NP
authors.lastname: Day
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
mesh_terms: D000483:Alleles; D000818:Animals; D023482:Bioterrorism; D042726:Burkholderia mallei / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005896:Glanders / Q000382:microbiology*; D006736:Horses; D006801:Humans; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012703:Serotyping
chemical_list: D004269:DNA, Bacterial