title: Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit.
abstract: The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcepsilonRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.
authors.affiliation: San Raffaele Scientific Institute, via Olgettina 58, 20132 Milan, Italy. s.burastero@hsr.it
authors.forename: S E
authors.initials: SE
authors.lastname: Burastero
authors.forename: C
authors.initials: C
authors.lastname: Paolucci
authors.forename: D
authors.initials: D
authors.lastname: Breda
authors.forename: G
authors.initials: G
authors.lastname: Monasterolo
authors.forename: R E
authors.initials: RE
authors.lastname: Rossi
authors.forename: L
authors.initials: L
authors.lastname: Vangelista
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001491:Basophils / Q000378:metabolism*; D015550:Cell Degranulation / Q000276:immunology; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005189:False Positive Reactions; D006801:Humans; D015289:Leukotrienes / Q000378:metabolism*; D051379:Mice; D011933:Reagent Kits, Diagnostic / Q000592:standards; D015203:Reproducibility of Results
chemical_list: D000911:Antibodies, Monoclonal; D015289:Leukotrienes; D011933:Reagent Kits, Diagnostic