Crystallization and preliminary crystallographic studies of an antitumour lectin from the edible mushroom Agrocybe aegerita.
An antitumour lectin named AAL has been purified from the fruiting body of edible mushroom Agrocybe aegerita. In addition to having a distinct bioactivity, AAL shows strong inhibition effects on human and mouse tumour cells. It has been shown that AAL exerts its antitumour effects via apoptosis-induction. AAL and AAL-lactose complex have been crystallized and their diffraction data were collected with resolution of 2.6 A and 3.0 A, respectively. Both crystals belong to space group P 6122 with unit cell parameters a = 123.98 A, b = 123.98 A, c = 56.86 A, alpha= beta=90 degrees, gamma = 120 degrees and a = 123.69 A, b = 123.69 A, c = 56.64 A, alpha = beta = 90 degrees, gamma = 120 degrees, respectively.
Yang
Na
N
Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
Liang
Yi
Y
Xiang
Ye
Y
Zhang
Ying
Y
Sun
Hui
H
Wang
Da-Cheng
DC
D000363:Agaricales / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005502:Food; D037102:Lectins / Q000737:chemistry*
D000970:Antineoplastic Agents; D037102:Lectins