[Changes of gene expression profile of multiple myeloma cell line RPMI 8226 treated by arsenic trioxide].
OBJECTIVE
To compare the changes of gene expression profiles of multiple myeloma cell line RPMI 8226 before and after 24-hour intervention of arsenic trioxide.


METHODS
The responses of the RPMI 8226 cells to arsenic trioxide were determined with cDNA microarray which included 4,096 different human genes.


RESULTS
Of these 4,096 genes, the expressions of 273 genes were altered significantly at mRNA level. The expressions of 121 genes were up-regulated while the expressions of 152 genes were down-regulated.


CONCLUSIONS
The effect of arsenic trioxide on RPMI 8226 cells is related to changing the expression levels of a number of genes. ZFYVE16, ALK1 and TXNIP genes may play important roles in apoptosis and differentiation of RPMI 8226 cells.
Wang
Meng-Chang
MC
Department of Hematology, First Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710061, China. Swallow3956@sina.com
Liu
Shan-Xi
SX
Liu
Peng-Bo
PB
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006801:Humans; D009101:Multiple Myeloma / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D010087:Oxides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D000077237:Arsenic Trioxide