title: Crystallization and preliminary structure analysis of the blue laccase from the ligninolytic fungus Panus tigrinus.
abstract: The blue laccase from the white-rot basidiomycete fungus Panus tigrinus, an enzyme involved in lignin biodegradation, has been crystallized. P. tigrinus laccase crystals grew within one week at 296 K using the sitting-drop vapour-diffusion method in 22%(w/v) PEG 4000, 0.2 M CaCl2, 100 mM Tris-HCl pH 7.5. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 54.2, b = 111.6, c = 97.1, beta = 97.7 degrees , and contain 46% solvent. A complete native data set was collected to 1.4 A resolution at the copper edge. Molecular replacement using the Coprinus cinereus laccase structure (PDB code 1hfu) as a starting model was performed and initial electron-density maps revealed the presence of a full complement of copper ions. Model refinement is in progress. The P. tigrinus laccase structural model exhibits the highest resolution available to date and will assist in further elucidation of the catalytic mechanism and electron-transfer processes for this class of enzymes.
authors.affiliation: Department of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Ferraroni
authors.forename: Ilaria
authors.initials: I
authors.lastname: Duchi
authors.forename: Nina M
authors.initials: NM
authors.lastname: Myasoedova
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Leontievsky
authors.forename: Ludmila A
authors.initials: LA
authors.lastname: Golovleva
authors.forename: Andrea
authors.initials: A
authors.lastname: Scozzafava
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Briganti
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification; D042845:Laccase / Q000737:chemistry* / Q000302:isolation & purification; D008031:Lignin / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: D005656:Fungal Proteins; D008031:Lignin; D042845:Laccase