title: Crystallization and preliminary X-ray crystallographic analysis of a conserved domain in plants and prokaryotes from Pyrococcus horikoshii OT3.
abstract: A plant- and prokaryote-conserved domain (PPC) has previously been found in AT-hook motif nuclear localized protein 1 (AHL1) localized in the nuclear matrix of Arabidopsis thaliana (AtAHL1). AtAHL1 has a DNA-binding function. Mutation analyses of AtAHL1 has previously revealed that the hydrophobic region of the PPC domain is essential for its nuclear localization. In this study, the PPC of the hyperthermophilic archaebacterium Pyrococcus horikoshii (PhPPC) was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to the hexagonal space group P6(3)22, with unit-cell parameters a = b = 53.69, c = 159.2 A. Data were obtained at 100 K, with diffraction being observed to a resolution of 1.7 A. A complete data set from crystals of the SeMet-substituted protein was also obtained.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871 Osaka, Japan.
authors.forename: Linyen
authors.initials: L
authors.lastname: Lin
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakano
authors.forename: Susumu
authors.initials: S
authors.lastname: Uchiyama
authors.forename: Satoru
authors.initials: S
authors.lastname: Fujimoto
authors.forename: Sachihiro
authors.initials: S
authors.lastname: Matsunaga
authors.forename: Shota
authors.initials: S
authors.lastname: Nakamura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Tadayasu
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Kiichi
authors.initials: K
authors.lastname: Fukui
mesh_terms: D020816:Amino Acid Motifs; D029681:Arabidopsis Proteins / Q000737:chemistry*; D017124:Conserved Sequence; D003460:Crystallization / Q000379:methods; D004268:DNA-Binding Proteins / Q000737:chemistry*; D010944:Plants / Q000737:chemistry; D011387:Prokaryotic Cells / Q000737:chemistry; D043902:Pyrococcus horikoshii / Q000737:chemistry*; D012645:Selenomethionine; D014961:X-Ray Diffraction
chemical_list: C496143:AHL1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D012645:Selenomethionine