Analysis of the CDR3 length of TCR alphabeta T cells in the peripheral blood of patients with chronic hepatitis B.
To clarify the characteristics of TCR alphabeta T cells in the peripheral blood of patients with chronic hepatitis B (CHB), we investigated the patterns of Complementarity Determining Region3 (CDR3) length distribution for all 24 TCR BV gene families and 32 TCR AV gene family in the peripheral blood lymphocytes of two patients with CHB and two healthy controls by immunoscope spectratyping technique. We found that the profiles of CDR3 length distribution for all TCR AV and TCR BV family showed Gaussian distribution (the polyclonal TCR alphabeta T cells) in healthy controls, however, the restricted usage of TCR BV and TCR AV family (the oligoclonal TCR alphabeta T cells) has been monitored in two CHB patients, furthermore, the oligoclonal TCR alphabeta T cells showed the different CDR3 sequences and length, it might be correlated to the different epitope of hepatitis B virus (HBV) or the different HLA type of the patients. Detailed analysis of the CDR3 length of TCR alphabeta T cells might be interesting to light on the further study of the individualized immunotherapy of CHB and the further research of the new T lymphocyte epitope vaccine of HBV.
Yao
Xin-Sheng
XS
Institute of Molecular Immunology, Southern Medical University (The First Military Medical University), GuangZhou 510515, PR China.
Zhang
Guang-Wen
GW
Ma
Li
L
Wen
Qian
Q
Hou
Jin-Lin
JL
Meng
Min-Jie
MJ
Ma
Shi-Wu
SW
Liang
Min-Feng
MF
Lin
Ying
Y
Wu
Zhen-Qiang
ZQ
He
Xiao-Wei
XW
Wang
Ju-Fang
JF
Wang
Xiao-Ning
XN