Fluorescent nanoparticles as selective Cu(II) sensors.
Latex nanoparticles functionalized with cyclam (1,4,8,11-tetraazacyclotetradecane), a copper chelator, have been doped with a fluorescent dye (BODIPY derivative: 4,4-difluoro-8-(2',4',6'-trimethyl)phenyl-2,6-diethyl-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene). The bulky, hydrophobic fluorophore dissolves within the nanoparticles' polymer core up to a concentration of about 88.4 micromol g(-1). At this concentration the fluorescence yield is about 0.80. Adding Cu2+ ions to the solution decreases the fluorescence because of the energy transfer between the dye and the violet copper cyclam complexes. The response is fast: 90% of the quenching occurs within 1 s. The Cu2+ detection threshold is of 1 nanomolar. No interferences were observed with zinc and nickel ions.
Méallet-Renault
Rachel
R
Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires, CNRS UMR 8531, Ecole Normale Supérieure de Cachan, 94235, Cachan Cedex, France. rachelm@ppsm.ens-cachan.fr
Hérault
Alexandre
A
Vachon
Jean-Jacques
JJ
Pansu
Robert B
RB
Amigoni-Gerbier
Sonia
S
Larpent
Chantal
C
D001896:Boron Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D004735:Energy Transfer; D005454:Fluorescence Polarization / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D006571:Heterocyclic Compounds / Q000737:chemistry*; D006575:Heterocyclic Compounds, 3-Ring / Q000138:chemical synthesis / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
C511354:4,4-difluoro-8-(2',4',6'-trimethyl)phenyl-2,6-diethyl-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D002614:Chelating Agents; D005456:Fluorescent Dyes; D006571:Heterocyclic Compounds; D006575:Heterocyclic Compounds, 3-Ring; C024560:cyclam; D003300:Copper