Isolation and purification of oridonin from Rabdosia rubescens using upright counter-current chromatography.
A preparative counter-current chromatography (CCC) method for isolation and purification of oridonin, a new cancer chemoprevention agent, from the Chinese medicinal plant Rabdosia rubescens was successfully established. The crude oridonin was obtained by elution with a light petroleum/acetone solvent mixture from ethanol extracts of R. rubescens using column chromatography on silica gel. With a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (1:2:1:2, v/v), 120 mg of oridonin at the purity of 97.8% was obtained from 200 mg of the crude sample in a single-step CCC separation. The structure of oridonin was identified by ESI-MS, 1H NMR, and 13C NMR.
Lu
Yanbin
Y
Department of Chemistry, Zhejiang University, Hangzhou, 310027, China.
Sun
Cuirong
C
Pan
Yuanjiang
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D037602:Isodon / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012997:Solvents
D004224:Diterpenes; D045786:Diterpenes, Kaurane; D004365:Drugs, Chinese Herbal; D012997:Solvents; C011959:oridonin