title: In vivo imaging of the dendritic arbors of layer V pyramidal cells in the cerebral cortex using a laser scanning microscope with a stick-type objective lens.
abstract: In the field of neuroscience, low-invasive in vivo imaging would be a very useful method of monitoring the morphological dynamics of intact neurons in living animals. At present, there are two widely used in vivo imaging methods; one is the two-photon microscope method, and the other is the fiber optics method. However, these methods are not suitable for the in vivo imaging of deeper subcortical structures. In our study, we have developed a novel method for the in vivo imaging of pyramidal neurons in layer V of the cerebral cortex, utilizing a MicroLSM system and a stick-type objective lens that can be directly inserted into the target tissue. By using this method, we succeeded in obtaining clear images of pyramidal neurons in layer V of the cerebral cortex under a low-invasive condition. The MicroLSM system is a useful and versatile in vivo imaging system that will be applicable not only to the brain but also to other organs.
authors.affiliation: Laboratory for Molecular Gerontology, Mitsubishi Kagaku Institute of Life Sciences Setou Group, Machida 194-8511, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Fukuda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawano
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Tanikawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Oba
authors.forename: Masafumi
authors.initials: M
authors.lastname: Koyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takagi
authors.forename: Mineo
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Nagayama
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002540:Cerebral Cortex / Q000166:cytology*; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure*; D003952:Diagnostic Imaging / Q000379:methods; D007909:Lenses; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000295:instrumentation*; D017966:Pyramidal Cells / Q000166:cytology*
chemical_list: D008164:Luminescent Proteins