Design and characterization of magnetic resonance imaging gene reporters.
We review the current status for magnetic resonance contrast agents that are designed to act as gene reporters. The basic concepts behind magnetic resonance and contrast enhancement are discussed, and factors that influence design of activatable contrast agents are presented. Several designs for magnetic resonance imaging (MRI) gene reporters are described, including contrast agents that are activated by beta-galactosidase, and marker genes that code for proteins that sequester iron. Methods to characterize the uptake and delivery of contrast agents are outlined.
Louie
Angelique
A
Department of Biomedical Engineering, University of California, Davis, USA.
D000818:Animals; D003287:Contrast Media; D015195:Drug Design; D020869:Gene Expression Profiling / Q000379:methods*; D018390:Gene Targeting / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D015202:Protein Engineering / Q000379:methods
D003287:Contrast Media