Presentation of the HCV-derived lipopeptide LP20-44 by dendritic cells enhances function of in vitro-generated CD4+ T cells via up-regulation of TLR2.
In vitro immunization with HCV lipopeptide aa 20-44 results in antigen-specific T cells. Here, we studied whether presentation of the lipopeptide by dendritic cells (DC) results in enhanced T cell functions. DC-immunized T cells showed significantly augmented proliferation and IFN-gamma secretion upon HCV-specific re-stimulation. This effect was related to significantly increased expression of TLR2 on DC-immunized CD4+ T cells and required TLR2 triggering during re-stimulation. In contrast, numbers of HLA-A2-pentamer-positive CD8+ T cells and granzyme B-secreting T cells remained unchanged. Thus, up-regulation of TLR2 during immunization with DC enhances functions of CD4+ T cells but not CD8+ T cells.
Langhans
Bettina
B
Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53105 Bonn, Germany. bettina.langhans@ukb.uni-bonn.de
Braunschweiger
Ingrid
I
Nischalke
Hans-Dieter
HD
Nattermann
Jacob
J
Sauerbruch
Tilman
T
Spengler
Ulrich
U
D000276:Adjuvants, Immunologic; D000328:Adult; D017951:Antigen Presentation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D003545:Cysteine / Q000031:analogs & derivatives; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D053804:Granzymes; D016174:Hepacivirus / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D008074:Lipoproteins / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012697:Serine Endopeptidases / Q000032:analysis; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis*; D015854:Up-Regulation; D014764:Viral Proteins / Q000737:chemistry / Q000276:immunology*
D000276:Adjuvants, Immunologic; D008074:Lipoproteins; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2; D014764:Viral Proteins; D007371:Interferon-gamma; C038837:tripalmitoyl cysteine; C509213:GZMB protein, human; D053804:Granzymes; D012697:Serine Endopeptidases; D003545:Cysteine