title: Crystallization and preliminary X-ray diffraction analysis of a new chitin-binding protein from Parkia platycephala seeds.
abstract: A chitin-binding protein named PPL-2 was purified from Parkia platycephala seeds and crystallized. Crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 55.19, b = 59.95, c = 76.60 A, and grew over several days at 293 K using the hanging-drop method. Using synchrotron radiation, a complete structural data set was collected to 1.73 A resolution. The preliminary crystal structure of PPL-2, determined by molecular replacement, presents a correlation coefficient of 0.558 and an R factor of 0.439. Crystallographic refinement is in progress.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Biomol-Lab, CEP 60451-970, Caixa Postal 6043, Fortaleza-Ceará, Brazil. bscavada@ufc.br
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.forename: Rolando E R
authors.initials: RE
authors.lastname: Castellón
authors.forename: Georg G
authors.initials: GG
authors.lastname: Vasconcelos
authors.forename: Bruno A M
authors.initials: BA
authors.lastname: Rocha
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Bezerra
authors.forename: Henri
authors.initials: H
authors.lastname: Debray
authors.forename: Plínio
authors.initials: P
authors.lastname: Delatorre
authors.forename: Celso S
authors.initials: CS
authors.lastname: Nagano
authors.forename: Marcos
authors.initials: M
authors.lastname: Toyama
authors.forename: Vicente P T
authors.initials: VP
authors.lastname: Pinto
authors.forename: Frederico B M B
authors.initials: FB
authors.lastname: Moreno
authors.forename: Fernanda
authors.initials: F
authors.lastname: Canduri
authors.forename: Walter F de
authors.initials: WF
authors.lastname: Azevedo
mesh_terms: D000595:Amino Acid Sequence; D002686:Chitin / Q000378:metabolism*; D003460:Crystallization; D007887:Fabaceae / Q000737:chemistry*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry*; D011485:Protein Binding; D012639:Seeds / Q000737:chemistry*; D016415:Sequence Alignment; D014961:X-Ray Diffraction
chemical_list: D010940:Plant Proteins; D002686:Chitin