title: Stability of selected hematology variables in canine blood kept at room temperature in EDTA for 24 and 48 hours.
abstract: BACKGROUND
  Most hematologic analyses are performed within a short time of blood sampling, but samples collected at the end of a week may have to be stored for up to 2 days. The stability of hematologic constituents is poorly documented.
  
  
  OBJECTIVE
  The objective of this study was to compare the results of RBC, WBC and platelet counts, hemoglobin (Hgb) concentration, and MCV before and after storage of canine blood at room temperature for 24 and 48 hours.
  
  
  METHODS
  One hundred fifty-two K3-EDTA canine blood specimens from 2 veterinary hospitals were analyzed within 4 hours of collection, then 24 and 48 hours later with a Coulter T540 hematology analyzer. Results were compared by Passing-Bablock agreement, difference plots, and according to their classification as normal or abnormal based on reference intervals.
  
  
  RESULTS
  RBC count and Hgb concentration were stable for the duration of the study. Differences in WBC and platelet counts varied with the specimen, independently of the initial value. MCV increased consistently over the 2 days. However, only a few results were misclassified.
  
  
  CONCLUSIONS
  Whole blood specimens stored for up to 2 days at room temperature are suitable for cell counts and Hgb measurement. However, potential variations have to be known to avoid misinterpretations, especially near the decision limits.
authors.affiliation: Laboratoire Vebiotel, Arcueil, France. christine.medaille@wanadoo.fr
authors.forename: C
authors.initials: C
authors.lastname: Médaille
authors.forename: A
authors.initials: A
authors.lastname: Briend-Marchal
authors.forename: J P
authors.initials: JP
authors.lastname: Braun
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs / Q000097:blood*; D004492:Edetic Acid / Q000737:chemistry / Q000494:pharmacology*; D004906:Erythrocyte Count / Q000662:veterinary; D005260:Female; D006454:Hemoglobins; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D013696:Temperature; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D006454:Hemoglobins; D004492:Edetic Acid