title: Bluetongue virus: (1) in pregnant white-tailed deer (2) a plaque reduction neutralization test.
abstract: Six white-tailed deer (Odocoileus virginianus) were infected with bluetongue virus (BTs, vaccinal strain) approximately one-third of the way through their gestation period. One deer died of bluetongue 21 days after inoculation. Of the five surviving the infection, one had two mummified fetuses, and the others no fetuses upon euthanasia two weeks after term. Fetuses were present in two control deer and in the one which died of bluetongue. A plaque reduction neutralization test for bluetongue virus was developed and described for the first time and its sensitivity illustrated by high post inoculation titers which ranged from 1:3200 to greater than 1:16000.
authors.affiliation: Department of Veterinary Science, University of Wisconsin, Madison, Wisconsin 53706, USA.
authors.forename: F C
authors.initials: FC
authors.lastname: Thomas
authors.forename: D O
authors.initials: DO
authors.lastname: Trainer
mesh_terms: D000034:Abortion, Veterinary / Q000662:veterinary / Q000821:virology; D000818:Animals; D001819:Bluetongue / Q000175:diagnosis*; D001820:Bluetongue virus / Q000302:isolation & purification* / Q000472:pathogenicity; D003670:Deer / Q000821:virology*; D005260:Female; D009500:Neutralization Tests / Q000379:methods / Q000662:veterinary*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D019562:Viral Load / Q000662:veterinary