Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping.
Effective methods for manipulating, isolating and sorting cells and particles are essential for the development of microfluidic-based life science research and diagnostic platforms. We demonstrate an integrated optical platform for cell and particle sorting in microfluidic structures. Fluorescent-dyed particles are excited using an integrated optical waveguide network within micro-channels. A diode-bar optical trapping scheme guides the particles across the waveguide/micro-channel structures and selectively sorts particles based upon their fluorescent signature. This integrated detection and separation approach streamlines microfluidic cell sorting and minimizes the optical and feedback complexity commonly associated with extant platforms.
Applegate
Robert W
RW
Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, CO 80401, USA. rapplega@mines.edu
Squier
Jeff
J
Vestad
Tor
T
Oakey
John
J
Marr
David W M
DW
Bado
Philippe
P
Dugan
Mark A
MA
Said
Ali A
AA
D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003102:Colloids / Q000737:chemistry; D004867:Equipment Design; D005434:Flow Cytometry / Q000295:instrumentation*; D007834:Lasers; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics; D010316:Particle Size; D012680:Sensitivity and Specificity; D018511:Systems Integration
D003102:Colloids