title: Cryopreservation of preantral ovarian follicles in situ from domestic cats (Felis catus) using different cryoprotective agents.
abstract: The objective of this investigation was to verify the structural characteristics of preantral follicles (PAF) of cat ovarian tissue after cryopreservation in 1.5 M glycerol or ethylene glycol, using a slow-freezing procedure. Ovaries (n = 10) from domestic cats were divided into fragments. One fragment was immediately preserved for classical histology (fresh control), and additional fragments were immersed in minimum essential medium plus 10% bovine fetal serum (MEM+BFS), or MEM+BFS supplemented with 1.5 M glycerol or ethylene glycol. The samples were frozen and plunged into liquid nitrogen. After 1 wk, the samples were thawed. A total of 600 PAF were evaluated. In the fresh control, there were 71.3% normal PAF. After thawing, the rates of normal PAF were 26.0, 39.3 and 58.0% for samples without cryoprotectant or with glycerol or ethylene glycol, respectively. We concluded that ethylene glycol was useful for the cryopreservation of feline PAF in situ.
authors.affiliation: Laboratory of Carnivore Reproduction - PPGCV/UECE, Paranjana Ave. 1700, Itaperi, Fortaleza-Ceará 60740-000, Brazil. ana_kelen@yahoo.com.br
authors.forename: Ana Kelen F
authors.initials: AK
authors.lastname: Lima
authors.forename: Alexandre R
authors.initials: AR
authors.lastname: Silva
authors.forename: Regiane R
authors.initials: RR
authors.lastname: Santos
authors.forename: Daniele M
authors.initials: DM
authors.lastname: Sales
authors.forename: Andreia F
authors.initials: AF
authors.lastname: Evangelista
authors.forename: José R
authors.initials: JR
authors.lastname: Figueiredo
authors.forename: Lúcia D M
authors.initials: LD
authors.lastname: Silva
mesh_terms: D000818:Animals; D002415:Cats / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D003451:Cryoprotective Agents; D019855:Ethylene Glycol; D005260:Female; D005990:Glycerol; D006651:Histocytochemistry / Q000662:veterinary; D006080:Ovarian Follicle
chemical_list: D003451:Cryoprotective Agents; D019855:Ethylene Glycol; D005990:Glycerol