Novel method for aroma recovery from the bioconversion of lutein to beta-ionone by Trichosporon asahii using a mesoporous silicate material.
In this work, we report on the synthesis and ability of the mesoporous material MCM-41 to adsorb the norisoprenoid beta-ionone. This compound, with a violet aroma note, can be produced from lutein by the yeast Trichosporon asahii through a bioconversion process. We found that beta-ionone inhibited the yeast growth and constrained aroma formation. Growth inhibition was overcome using silicate MCM-41 as sorbent device in a fermentation system that allowed product removal from the culture medium by headspace manipulation. Compared to a commercial silica gel, the mesoporous material exhibited a 4.5-fold higher beta-ionone adsorption. Contrasting to cultures without the sorbent device, the presence of MCM-41 allowed a marked increase (14-fold) in beta-ionone production. Our results suggested that confinement of the norisoprenoid into the sorbent material bypassed its toxicity which allowed a better beta-ionone production. This study represents the first report on the use of MCM-41 to recover an aroma produced by fermentation and therefore, a novel application for a mesoporous material.
Rodríguez-Bustamante
E
E
Departamento de Biología Molecular y Biotecnología del Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, DF 04510, Mexico. erodriguezb@gmail.com
Maldonado-Robledo
G
G
Sánchez-Contreras
A
A
Klimova
T
T
Arreguín-Espinosa
R
R
Sánchez
S
S
D000327:Adsorption; D014975:Lutein / Q000737:chemistry* / Q000378:metabolism; D045792:Norisoprenoids / Q000737:chemistry* / Q000378:metabolism; D009812:Odorants; D014250:Trichosporon / Q000378:metabolism*; D017641:Zeolites / Q000737:chemistry*
D045792:Norisoprenoids; D017641:Zeolites; C008157:beta-ionone; D014975:Lutein