title: Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis.
abstract: Quantitative culture, quantitative PCR and the galactomannan enzyme immunoassay (EIA) were compared for their ability to determine the pulmonary fungal burden in a murine model of invasive aspergillosis. Quantitative culture of specimens containing hyphae under-represented the absolute fungal burden in established infection when compared with the two other methods. The best correlation was observed between the two non-culture methods. Higher variability was observed with the galactomannan EIA when compared with quantitative PCR. Collectively, these data suggest that quantitative PCR is the preferred method for determination of the pulmonary fungal burden in experimental aspergillosis.
authors.affiliation: Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada. donald.sheppard@mcgill.ca
authors.forename: D C
authors.initials: DC
authors.lastname: Sheppard
authors.forename: K A
authors.initials: KA
authors.lastname: Marr
authors.forename: D N
authors.initials: DN
authors.lastname: Fredricks
authors.forename: L Y
authors.initials: LY
authors.lastname: Chiang
authors.forename: T
authors.initials: T
authors.lastname: Doedt
authors.forename: S G
authors.initials: SG
authors.lastname: Filler
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000382:microbiology*; D001230:Aspergillus / Q000302:isolation & purification*; D046508:Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D008168:Lung / Q000382:microbiology; D008172:Lung Diseases, Fungal / Q000382:microbiology*; D051379:Mice; D016133:Polymerase Chain Reaction