[Generation of Ayu17-449 gene knockout mice by gene trapping].
We report the generation of Ayu17-449 gene knockout mice in an effort to facilitate studies of its function during mouse development, A special gene trapping vector was transfected into mouse ES cells. The clone with the trapped gene trap was identified by 5' RACE and Southern blot analysis. Ayu17-449 trapped mice were then generated by using this ES clone. In addition, the expression of Ayu17-449 in this mutant line was analyzed with Northern blot. Results showed that in these mutant mice, the trapping vector was located upstream of the Ayu17-449 initiation site, and as a result, the transcription of Ayu17-449 gene was inhibited. Ayu17-449 trapped mice are a useful tool for the analysis of Ayu17-449 gene function in mouse development.
Tang
Hua
H
Key Laboratory of Molecular Infectious Diseases, Chongqing University of Medical Sciences, Chongqing 400016, China. tanghua86162003@yahoo.com.cn
Tang
Ren-Kuan
RK
Huang
Ai-Long
AL
Araki
Kimi
K
Yamamura
Kenichi
K
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D005796:Genes / Q000235:genetics*; D005838:Genotype; D051379:Mice; D018345:Mice, Knockout; D016133:Polymerase Chain Reaction