[Mechanisms of peroxidase oxidation of o-dianisidine, 3,3',5,5'-tetramethylbenzidine, and o-phenylenediamine in the presence of sodium dodecyl sulfate].
Peroxidase oxidation of o-dianisidine, 3,3',5,5'-tetramethylbenzidine, and o-phenylenediamine in the presence of sodium dodecyl sulfate (SDS), an anionic surfactant, was spectrophotometrically studied. It was found that 0.1-100 mM SDS concentrations stabilize intermediates formed in the peroxidase oxidation of these substrates. The cause of the stabilization is an electrostatic interaction between positively charged intermediates and negatively charged surfactant.
Kireĭko
A V
AV
Veselova
I A
IA
Shekhovtsova
T N
TN
D001560:Benzidines / Q000737:chemistry*; D003962:Dianisidine / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D010084:Oxidation-Reduction; D010655:Phenylenediamines / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods
D001560:Benzidines; D010655:Phenylenediamines; D012967:Sodium Dodecyl Sulfate; C021758:3,3',5,5'-tetramethylbenzidine; C034193:1,2-diaminobenzene; D006735:Horseradish Peroxidase; D003962:Dianisidine