Protective effect of panaxydol and panaxynol on sodium nitroprusside-induced apoptosis in cortical neurons.
An excess of the free radical nitric oxide (NO) is viewed as a deleterious factor involved in various CNS disorders. The protective effect of panaxydol (PND) and panaxynol (PNN) on sodium nitroprusside (SNP)-induced neuronal apoptosis and potential mechanism were investigated in primary cultured rat cortical neurons. Pretreatment of the cells with PND or PNN for 24 h following 1mM SNP, an exogenous NO donor, exposure for 1h, resulted significantly in reduction of cell death induced by SNP determined by MTT assay, LDH release and Hoechst staining. 5 microM PND and PNN also reduced the up-regulation of the pro-apoptotic gene, Bax, down-regulation of the anti-apoptotic gene, Bcl-2. The observations demonstrated that PND and PNN protect neurons against SNP-induced apoptosis via regulating the apoptotic related genes. The results raise the possibility that PND and PNN reduce neurodegeneration in the Alzheimer's brain.
Nie
Bao-Ming
BM
Institute of Pharmaceutical Research, School of Medicine, Shanghai Jiao Tong University, PR China.
Yang
Li-Min
LM
Fu
Sai-Li
SL
Jiang
Xiao-Yan
XY
Lu
Pei-Hua
PH
Lu
Yang
Y
D000480:Alkynes / Q000494:pharmacology; D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D053280:Diynes; D005233:Fatty Alcohols / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D009599:Nitroprusside / Q000633:toxicity*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000480:Alkynes; D053280:Diynes; D005233:Fatty Alcohols; D005609:Free Radicals; D020011:Protective Agents; D009599:Nitroprusside; C018541:falcarinol; D009569:Nitric Oxide; C055778:panaxydol