[Primary culture and morphologic observation of eutopic and ectopic endometrial cells from patients with endometriosis].
OBJECTIVE
To explore the method of primary culture for endometriotic cells and to find out the differences in morphological manifestations among endometriotic cells and eutopic endometrial cells sampled from patients with endometriosis and endometriosis-free women.


METHODS
Endometriotic and eutopic endometrial cells were cultured by modified method of primary culture. The endometriotic cell types were observed and differentiated under optical and electron microscopes.


RESULTS
The success rates for culture of eutopic endometrial cells from endometriosis-free women and patients with endometriosis were 91.67% and 93.75% respectively. The success rate for culture of endometriotic cells was 75.00%. The size of endometriotic glandular cells was similar to those of eutopic endometrial glandular cells from endometriosis-free women and patients with endometriosis. The chromatin was manifold and the nucleus was augmented in the endometriotic glandular cells. The endometriotic stromal cells were smaller than the eutopic endometrial stromal cells from endometriosis-free women and patients with endometriosis. Many tiny villi and protuberances on plasma membrane could be seen in the endometriotic stromal cells.


CONCLUSIONS
The success rate for culture of endometriotic cells can be elevated through improving the method of primary culture. The ultrastructures of endometriotic glandular and stromal cells are obviously different from those of eutopic endometrial glandular and stromal cells from endometriosis-free women and patients with endometriosis.
Yu
Chao-Qin
CQ
Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. chqyu81@gmail.com
Shi
Shu-Fang
SF
Liu
Yu-Huan
YH
Wang
Rui-Xia
RX
Song
Yan-Hua
YH
Yu
Jin
J
D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004715:Endometriosis / Q000473:pathology*; D004717:Endometrium / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D008875:Middle Aged