Crystallization and preliminary X-ray analysis of the Man(alpha1-2)Man-specific lectin from Bowringia mildbraedii in complex with its carbohydrate ligand.
The lectin from Bowringia mildbraedii seeds crystallizes in the presence of the disaccharide Man(alpha1-2)Man. The best crystals grow at 293 K within four weeks after a pre-incubation at 277 K to induce nucleation. A complete data set was collected to a resolution of 1.90 A using synchrotron radiation. The crystals belong to space group I222, with unit-cell parameters a = 66.06, b = 86.35, c = 91.76 A, and contain one lectin monomer in the asymmetric unit.
Garcia-Pino
Abel
A
Department of Molecular and Cellular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
Loris
Remy
R
Wyns
Lode
L
Buts
Lieven
L
D000595:Amino Acid Sequence; D000818:Animals; D002241:Carbohydrates; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D004187:Disaccharides / Q000737:chemistry*; D007887:Fabaceae / Q000378:metabolism; D008358:Mannose / Q000737:chemistry*; D008362:Mannosides / Q000737:chemistry*; D015233:Models, Statistical; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry*; D011487:Protein Conformation; D011817:Rabbits; D012639:Seeds; D017386:Sequence Homology, Amino Acid; D017356:Synchrotrons; D013696:Temperature; D014965:X-Rays
D002241:Carbohydrates; D004187:Disaccharides; D008362:Mannosides; D037121:Plant Lectins; C475460:mannosyl alpha(1-6)-mannoside; D008358:Mannose