Crystallization and preliminary crystallographic analysis of an aminoglycoside kinase from Legionella pneumophila.
9-Aminoglycoside phosphotransferase type Ia [APH(9)-Ia] is a resistance factor in Legionella pneuemophila, the causative agent of legionnaires' disease. It is responsible for providing intrinsic resistance to the antibiotic spectinomycin. APH(9)-Ia phosphorylates one of the hydroxyl moieties of spectinomycin in an ATP-dependent manner, abolishing the antibiotic properties of this drug. Here, the crystallization and preliminary X-ray studies of this enzyme in two crystal forms is reported. One of the these crystal forms provides diffraction data to a resolution of 1.7 A.
Lemke
Christopher T
CT
Department of Microbiology and Immunology, McGill University, Canada.
Hwang
Jiyoung
J
Xiong
Bing
B
Cianciotto
Nicholas P
NP
Berghuis
Albert M
AM
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization / Q000379:methods; D019868:Kanamycin Kinase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016952:Legionella pneumophila / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D019868:Kanamycin Kinase