Ex vivo biochemical analysis of CFTR in human rectal biopsies.
This report describes the first biosynthetic analysis of the cystic fibrosis transmembrane conductance regulator (CFTR) in freshly excised human rectal biopsies. Expression of functional CFTR was assessed by intestinal current measurement (ICM) prior to biosynthetic studies. Several structural features of CFTR are found to be comparable to those established in CFTR-expressing cell lines. Interestingly, maturation of CFTR increases substantially in tissue incubated at 26 degrees C. Our data provide a solid basis for future studies on the characterisation of CFTR in pathological cases.
van Barneveld
Andrea
A
Klinische Forschergruppe, OE 6711, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, D-30623 Hannover, Germany. barneveld.andrea.van@mh-hannover.de
Stanke
Frauke
F
Ballmann
Manfred
M
Naim
Hassan Y
HY
Tümmler
Burkhard
B
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002217:Carbachol / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D005576:Colforsin / Q000494:pharmacology; D003545:Cysteine / Q000378:metabolism; D003550:Cystic Fibrosis / Q000473:pathology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D004594:Electrophysiology; D006632:Histamine / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D047468:Immunoprecipitation; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000737:chemistry / Q000473:pathology; D007553:Isotope Labeling; D008875:Middle Aged; D012007:Rectum / Q000737:chemistry* / Q000473:pathology; D013462:Sulfur Radioisotopes
D013462:Sulfur Radioisotopes; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005576:Colforsin; D006632:Histamine; D002217:Carbachol; D003545:Cysteine