Determination of Fc function with frozen red blood cells.
The Fc function of immunoglobulins is commonly determined by an assay based on monitoring immunoglobulin induced, complement mediated red cell lysis. This assay requires a continuous source of fresh red cells. We have shown that the assay can be successfully performed with frozen red cells. The possibility of access to a stored standard stock of red cells will improve the convenience of performing the assay and could contribute to improved assay reproducibility.
Gurevich
V
V
CSL Bioplasma, Research and Development, 189-209 Camp Road, Broadmeadows, Victoria 3047, Australia.
Bertolini
J
J
Lyons
K
K
D001681:Biological Assay; D001793:Blood Preservation; D015925:Cryopreservation; D004912:Erythrocytes / Q000187:drug effects*; D005615:Freezing; D006461:Hemolysis; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology / Q000494:pharmacology*
D007141:Immunoglobulin Fc Fragments