title: Purification and characterization of fenitrothion hydrolase from Burkholderia sp. NF100.
abstract: The organophosphorus pesticide hydrolase was purified to homogeneity from Burkholderia sp. NF100 by detergent extraction of the cell membrane fraction, anion-exchange, chromatofocusing, and gel filtration chromatographies. The purified enzyme had a molecular mass of 55 kDa and a pI 5.8, and the hydrolase activity was strongly inhibited by EDTA, dithiothreitol (DTT), Hg2+ and 1,10-phenanthroline. The optimum pH and temperature for the enzyme activity were 8.0 and 40 degrees C, respectively. The enzyme hydrolyzed five organophosphorus pesticides.
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu City, Gifu 501-1193, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Tago
authors.forename: Sumiko
authors.initials: S
authors.lastname: Yonezawa
authors.forename: Toshihide
authors.initials: T
authors.lastname: Ohkouchi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Masahito
authors.initials: M
authors.lastname: Hayatsu
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D019117:Burkholderia / Q000201:enzymology*; D002845:Chromatography; D004229:Dithiothreitol / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005278:Fenitrothion / Q000378:metabolism*; D006867:Hydrolases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D008628:Mercury / Q000494:pharmacology; D008970:Molecular Weight; D010575:Pesticides / Q000737:chemistry; D010618:Phenanthrolines / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D010575:Pesticides; D010618:Phenanthrolines; D004492:Edetic Acid; D006867:Hydrolases; D008628:Mercury; D004229:Dithiothreitol; C025205:1,10-phenanthroline; D005278:Fenitrothion