Oxidative damage to surfactant protein D in pulmonary diseases.
Surfactant protein D is an important innate host defence molecule that has been shown to interact with a variety of pathogens and to play a role in surfactant homeostasis. The aim of this study was to examine the influence of oxidation on surfactant protein D in different lung diseases. Bronchoalveolar lavage fluids (BALFs) from patients with different grade of protein oxidation were examined for changes in the primary chain and the quaternary structure of surfactant protein D. Significant changes of quaternary surfactant protein-D (SP-D) structure were detected under oxidative conditions in vitro and in vivo. The functional capacity of surfactant protein D to agglutinate bacteria was impaired by oxidation. We conclude that surfactant protein D is an important target of free radicals generated in the lungs. Host defence may be impaired due to the oxidation of surfactant protein D and may contribute to the suppurative lung diseases like cystic fibrosis (CF).
Starosta
Vitaliy
V
Lung Research Group, Children's Hospital of Ludwig Maximilians University, Munich, Germany.
Starosta
Vitality
V
Griese
Matthias
M
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D015153:Blotting, Western; D029481:Bronchitis, Chronic / Q000503:physiopathology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002648:Child; D002675:Child, Preschool; D006801:Humans; D018384:Oxidative Stress / Q000502:physiology*; D050050:Protein Carbonylation; D020836:Protein Structure, Quaternary; D037663:Pulmonary Surfactant-Associated Protein D / Q000737:chemistry* / Q000378:metabolism*
D037663:Pulmonary Surfactant-Associated Protein D