title: Polymorphisms in the canine glutamate transporter-1 gene: identification and variation among five dog breeds.
abstract: Excitatory amino acid transporters (EAATs) are important for terminating glutamatergic neurotransmission and protect central nervous system (CNS) neurons from glutamatergic excitotoxicity. We selected these genes as targets that may relate to canine behavioral traits. After screening four EAAT genes (glutamate transporter-1; GLT-1, excitatory amino acid transporter 4; EAAT4, excitatory amino acid carrier; EAAC1, glutamate/aspartate transporter; GLAST) for single nucleotide polymorphisms (SNPs), we identified two silent SNPs (C129T and T471C) in the GLT-1 gene. We genotyped 193 dogs of 5 breeds and found significant variation among breeds in these two SNPs in GLT-1. The C129T polymorphism was not observed in Malteses and Miniature Schnauzers. These results suggest that polymorphisms in the GLT-1 gene may be useful markers for examining how the genetic background relates to the behavioral traits of dogs.
authors.affiliation: Laboratory of Veterinary Ethology, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Niwako
authors.initials: N
authors.lastname: Ogata
authors.forename: Chie
authors.initials: C
authors.lastname: Hashizume
authors.forename: Yukihide
authors.initials: Y
authors.lastname: Momozawa
authors.forename: Koji
authors.initials: K
authors.lastname: Masuda
authors.forename: Takefumi
authors.initials: T
authors.lastname: Kikusui
authors.forename: Yukari
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Mori
mesh_terms: D000483:Alleles; D000818:Animals; D001522:Behavior, Animal; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D027342:Excitatory Amino Acid Transporter 2 / Q000737:chemistry / Q000235:genetics*; D014644:Genetic Variation; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D027342:Excitatory Amino Acid Transporter 2; D004247:DNA