title: Polymorphisms in the gene encoding hepatocyte nuclear factor-4alpha and susceptibility to type 2 diabetes in a Polish population.
abstract: Recently, several association studies of type 2 diabetes mellitus (T2DM) and the hepatocyte nuclear factor (HNF)-4alpha gene were reported with conflicting results. Our aim was to search for association between two polymorphisms of HNF-4alpha and T2DM in Polish Caucasians. The study groups comprised of 461 T2DM cases and 366 controls. Genotype-quantitative trait analyses were based on the oral glucose tolerance test (OGTT), glucose and insulin results, and comprised 310 glucose-tolerant subjects. All individuals were genotyped for two HNF-4alpha polymorphisms. The frequencies of the minor alleles were as follows: 19.2% in T2DM vs. 17.6% in controls for rs2144908; and 20.6% vs. 20.1% for rs4810424, respectively. The distributions of alleles, genotypes, and haplotypes of the HNF-4alpha polymorphisms did not differ between the study groups (lowest P = 0.41). None of the examined SNPs showed an association in control subjects with quantitative traits of fasting plasma glucose, fasting insulin, as well as plasma glucose and insulin 2 hours after glucose load in OGTT. We conclude that both examined polymorphisms in HNF-4alpha are not associated with T2DM and prediabetic phenotypes in Polish Caucasian study groups of this size.
authors.affiliation: Department of Metabolic Diseases, Medical College, Jagiellonian University, Krakow, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Wanic
authors.forename: M T
authors.initials: MT
authors.lastname: Malecki
authors.forename: P P
authors.initials: PP
authors.lastname: Wolkow
authors.forename: T
authors.initials: T
authors.lastname: Klupa
authors.forename: J
authors.initials: J
authors.lastname: Skupien
authors.forename: J
authors.initials: J
authors.lastname: Bobrek
authors.forename: E
authors.initials: E
authors.lastname: Kozek
authors.forename: A S
authors.initials: AS
authors.lastname: Krolewski
authors.forename: J
authors.initials: J
authors.lastname: Sieradzki
mesh_terms: D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000453:epidemiology* / Q000235:genetics; D004198:Disease Susceptibility; D005260:Female; D051557:Hepatocyte Nuclear Factor 4 / Q000097:blood*; D006801:Humans; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D007328:Insulin / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D011044:Poland / Q000453:epidemiology; D011110:Polymorphism, Genetic; D011236:Prediabetic State / Q000097:blood / Q000235:genetics; D012016:Reference Values
chemical_list: D001786:Blood Glucose; C496592:HNF4A protein, human; D051557:Hepatocyte Nuclear Factor 4; D007004:Hypoglycemic Agents; D007328:Insulin