title: Methods for isolation of cells from the human fetal-maternal interface.
abstract: During human pregnancy, fetal placental cells known as trophoblast invade into the uterine mucosal lining, coming into contact with maternal cells including a specialized population of leukocytes. In order to understand the interaction of maternal cells with trophoblast, it is useful to be able to isolate the various cell types from the fetal-maternal interface so that they may be characterized and co-cultured in vitro. This chapter details the methods we use in our laboratory for enrichment and/or purification of trophoblast cells, decidual leukocytes, decidual natural killer cells, decidual stromal cells, and decidual glandular epithelial cells from human first-trimester tissue samples.
authors.affiliation: Department of Pathology, University of Cambridge, United Kingdom.
authors.forename: Anita
authors.initials: A
authors.lastname: Trundley
authors.forename: Lucy
authors.initials: L
authors.lastname: Gardner
authors.forename: Jacquie
authors.initials: J
authors.lastname: Northfield
authors.forename: Chiwen
authors.initials: C
authors.lastname: Chang
authors.forename: Ashley
authors.initials: A
authors.lastname: Moffett
mesh_terms: D002469:Cell Separation / Q000379:methods*; D004847:Epithelial Cells; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural; D007962:Leukocytes; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D014327:Trophoblasts