Oligonucleotides with cyclohexene-nucleoside building blocks: crystallization and preliminary X-ray studies of a left-handed sequence GTGTACAC.
Cyclohexene nucleic acids contain a cyclohexene ring instead of the normal beta-D-2'-deoxyribose. The cyclohexene oligonucleotide GTGTACAC was synthesized using phosphoramidite chemistry and standard protecting groups. Crystals of GTGTACAC were obtained at 289 K by the hanging-drop vapour-diffusion technique. The crystals diffract to 1.7 A resolution and belong to the trigonal space group R3, with unit-cell parameters a = 41.434, c = 66.735 A.
Robeyns
Koen
K
Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B-3001 Heverlee, Belgium.
Herdewijn
Piet
P
Van Meervelt
Luc
L
D001483:Base Sequence; D003460:Crystallization / Q000379:methods; D003510:Cyclohexanes; D053138:Cyclohexenes; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D009943:Organophosphorus Compounds; D014835:Volatilization; D014961:X-Ray Diffraction
D003510:Cyclohexanes; D053138:Cyclohexenes; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; C052568:cyclohexene