title: Role of connective tissue growth factor in fibronectin synthesis in cultured human prostate stromal cells.
abstract: OBJECTIVE
  To clarify the correlation of connective tissue growth factor (CTGF) expression and fibronectin (FN) synthesis after transforming growth factor-beta-1 (TGF-beta1) stimulation in human prostate stromal cells.
  
  
  METHODS
  Primary cultures of human prostate stromal cells were established from nine normal prostates by the explant method. The gene and protein expressions of CTGF and FN after TGF-beta1 treatment were examined. We also investigated the effect of CTGF blockade on TGF-beta1-induced FN synthesis by CTGF antisense oligodeoxynucleotide treatment.
  
  
  RESULTS
  CTGF expression was detected in all nine prostate stromal cells by reverse transcriptase-polymerase chain reaction and immunoblotting. In prostatic tissues, CTGF expression was observed more strongly in epithelial cells than in the stromal area by immunohistochemistry. The upregulation of both CTGF and FN protein by TGF-beta1 treatment was demonstrated in a dose-dependent manner. CTGF antisense oligodeoxynucleotide inhibited TGF-beta1-stimulated FN synthesis.
  
  
  CONCLUSIONS
  CTGF plays a crucial role in extracellular matrix production as a TGF-beta1 downstream mediator in human prostate stromal cells, suggesting that CTGF blockade is likely to be a therapeutic target against benign prostatic hyperplasia.
authors.affiliation: Division of Urology, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Science, Niigata, Japan. kazuyas@med.niigata-u.ac.jp
authors.forename: Kazuya
authors.initials: K
authors.lastname: Suzuki
authors.forename: Kenji
authors.initials: K
authors.lastname: Obara
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Yamana
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bilim
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Itoi
authors.forename: Kota
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor; D005353:Fibronectins / Q000096:biosynthesis*; D006801:Humans; D017874:Immediate-Early Proteins / Q000502:physiology*; D018969:Insulin-Like Growth Factor Binding Proteins / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology*; D008297:Male; D011467:Prostate / Q000166:cytology* / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism
chemical_list: C526317:CCN2 protein, human; D005353:Fibronectins; D017874:Immediate-Early Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; D055513:Connective Tissue Growth Factor