title: Crystallization and preliminary X-ray diffraction analysis of a chitin-binding domain of hyperthermophilic chitinase from Pyrococcus furiosus.
abstract: The crystallization and preliminary X-ray diffraction analysis of the chitin-binding domain of chitinase from a hyperthermophilic archaeon, Pyrococcus furiosus, are reported. The recombinant protein was prepared using an Escherichia coli overexpression system and was crystallized by the hanging-drop vapour-diffusion method. An X-ray diffraction data set was collected to 1.70 A resolution. The crystal belonged to space group P4(3)2(1)2 or P4(1)2(1)2. The unit-cell parameters were determined to be a = b = 48.8, c = 85.0 A.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakamura
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishikawa
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hagihara
authors.forename: Takashi
authors.initials: T
authors.lastname: Oku
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakagawa
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ataka
authors.forename: Koichi
authors.initials: K
authors.lastname: Uegaki
mesh_terms: D002686:Chitin / Q000737:chemistry / Q000378:metabolism; D002688:Chitinases / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020027:Pyrococcus furiosus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D011994:Recombinant Proteins; D002686:Chitin; D002688:Chitinases