Localization of precursor proteins and mRNA of type I and III collagens in usual interstitial pneumonia and sarcoidosis.
The aim of this study was to assess and compare the accumulation and distribution of newly synthesized type I and III collagens in usual interstitial pneumonia (UIP) and pulmonary sarcoidosis. Lung biopsies from 10 patients with UIP and 13 patients with sarcoidosis were investigated by immunohistochemical technique and mRNA in situ hybridization. The antibodies for the aminoterminal propeptide of type I procollagen and the aminoterminal propeptide of type III procollagen (PINP and PIIINP, respectively) were used. When compared to healthy lung, levels of type I pN- and type III pN-collagens were increased in both of these disorders. Type I procollagen was mostly present as intracellular spots in newly formed fibrosis in UIP while type III pN-collagen was expressed extracellularly underneath metaplastic alveolar epithelium. Type I procollagen was present intracellularly within and around the granulomas of sarcoidosis, whereas type III pN-collagen was expressed extracellularly, mainly around the granulomas. mRNAs of both collagens colocalized with the precursor proteins. We conclude that the expression of precursor proteins and mRNA of type I and type III collagens is increased in UIP and sarcoidosis, reflecting mainly active synthesis of these collagens in different areas of the lung.
Kaarteenaho-Wiik
Riitta
R
Department of Pathology, University of Oulu, and Oulu University Hospital, Oulu, Finland. Riitta.Kaarteenaho-Wiik@oulu.fi
Lammi
Lauri
L
Lakari
Essi
E
Kinnula
Vuokko L
VL
Risteli
Juha
J
Ryhänen
Lasse
L
Pääkkö
Paavo
P
D024042:Collagen Type I / Q000235:genetics* / Q000378:metabolism*; D024061:Collagen Type III / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D017563:Lung Diseases, Interstitial / Q000235:genetics / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D021381:Protein Transport; D011650:Pulmonary Alveoli; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012507:Sarcoidosis / Q000235:genetics / Q000378:metabolism*
D024042:Collagen Type I; D024061:Collagen Type III; D011498:Protein Precursors; D012333:RNA, Messenger