Determination of domoic acid by on-line coupled capillary isotachophoresis with capillary zone electrophoresis.
An on-line coupled capillary isotachophoresis--capillary zone electrophoresis (cITP-CZE) method for the determination of domoic acid in shellfish and algae is described. The optimised cITP-CZE electrolyte system was 10 mM HCl + 20 mM beta-alanine (BALA) + 0.05% hydroxyethylcellulose (leading electrolyte), 5 mM caproic acid (terminating electrolyte) and 20 mM caproic acid + 20 mM BALA + 0.1% HPMC (background electrolyte). A clear separation of the domoic acid from the other components of methanolic sample extract was achieved within 25 min. Method characteristics, i.e., linearity (0-200 microg/l), accuracy (recovery 101+/-3%), intra-assay repeatability (2.4%) and detection limit (1.5 microg/l) were determined. Speed of analysis, low laboriousness, high sensitivity and low running cost are the typical attributes of the cITP-CZE method. Developed method was successfully applied to analysis of shellfish samples and food supplements containing algae extract.
Kvasnicka
Frantisek
F
Department of Food Preservation and Meat technology, Institute of Chemical Technology Prague, Technická 3, 166 28 Prague 6, Czech Republic. kvasnicf@vscht.cz
Sevcík
Rudolf
R
Voldrich
Michal
M
D002138:Calibration; D004586:Electrophoresis / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D056890:Eukaryota / Q000737:chemistry; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D012015:Reference Standards; D012758:Shellfish / Q000032:analysis
C012301:domoic acid; D007608:Kainic Acid