title: Effects of subminimum inhibitory concentrations of antibiotics on the Pasteurella multocida proteome.
abstract: Subminimum inhibitory concentrations (sub-MICs) of antibiotics can be therapeutically effective, but the underlying molecular mechanisms are not well-characterized. We analyzed the Pasteurella multocida proteome response to sub-MICs of amoxicillin, chlortetracycline, and enrofloxacin using isotope-coded affinity tags (ICAT). There were parallel effects on inhibition of growth kinetics and suppression of protein expression by clusters of orthologous groups (COG) categories. Potential compensatory mechanisms enabling antibiotic adaptation were identified, including increased RecA expression caused by enrofloxacin.
authors.affiliation: College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi 39762, USA.
authors.forename: Bindu
authors.initials: B
authors.lastname: Nanduri
authors.forename: Mark L
authors.initials: ML
authors.lastname: Lawrence
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Boyle
authors.forename: Mahalingam
authors.initials: M
authors.lastname: Ramkumar
authors.forename: Shane C
authors.initials: SC
authors.lastname: Burgess
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002751:Chlortetracycline / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000494:pharmacology; D016979:Pasteurella multocida / Q000187:drug effects* / Q000254:growth & development; D014176:Protein Biosynthesis / Q000187:drug effects; D020543:Proteome / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D020543:Proteome; D000077422:Enrofloxacin; D000658:Amoxicillin; D002751:Chlortetracycline