Compact soft x-ray transmission microscopy with sub-50 nm spatial resolution.
In this paper, the development of compact transmission soft x-ray microscopy (XM) with sub-50 nm spatial resolution for biomedical applications is described. The compact transmission soft x-ray microscope operates at lambda = 2.88 nm (430 eV) and is based on a tabletop regenerative x-ray source in combination with a tandem ellipsoidal condenser mirror for sample illumination, an objective micro zone plate and a thinned back-illuminated charge coupled device to record an x-ray image. The new, compact x-ray microscope system requires the fabrication of proper x-ray optical devices in order to obtain high-quality images. For an application-oriented microscope, the alignment procedure is fully automated via computer control through a graphic user interface. In imaging studies using our compact XM system, a gold mesh image was obtained with 45 nm resolution at x580 magnification and 1 min exposure. Images of a biological sample (Coscinodiscus oculoides) were recorded.
Kim
Kyong Woo
KW
Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University School of Medicine, Iksan, Jeonbuk 570-749, Korea.
Kwon
Youngman
Y
Nam
Ki-Yong
KY
Lim
Jong-Hyeok
JH
Kim
Kyu-Gyum
KG
Chon
Kwon Su
KS
Kim
Byoung Hoon
BH
Kim
Dong Eon
DE
Kim
JinGon
J
Ahn
Byoung Nam
BN
Shin
Hyun Joon
HJ
Rah
Seungyu
S
Kim
Ki-Ho
KH
Chae
Jin Seok
JS
Gweon
Dae Gab
DG
Kang
Dong Woo
DW
Kang
Sung Hoon
SH
Min
Jin Young
JY
Choi
Kyu-Sil
KS
Yoon
Seong Eon
SE
Kim
Eun-A
EA
Namba
Yoshiharu
Y
Yoon
Kwon-Ha
KH
D017377:Diatoms / Q000648:ultrastructure; D004577:Electron Probe Microanalysis / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008853:Microscopy; D046529:Microscopy, Electron, Transmission / Q000379:methods; D055096:Optical Devices; D017785:Photons; D012984:Software; D014965:X-Rays