Species identification of erythromycin-resistant Campylobacter isolates and optimization of a duplex PCR for rapid detection.
This study describes the approach used to verify the species identity of 23 erythromycin-resistant Campylobacter isolates whose identity was initially determined based mainly on the results of the rapid hippurate hydrolysis test or the results of the API-Campy identification system. Species identification of the isolates investigated was confirmed by repeating hippurate hydrolysis using a modification of the rapid hydrolysis test, in addition to performing three genetic-based assays. The original identification was verified in 69.6% of the isolates. The remaining isolates showed discrepancies in identity as determined by results of the identification assays performed. A duplex PCR assay, targeting the hipO and aspA genes, indicated the existence of mixed cultures of C. jejuni and C. coli in the frozen stocks of two of these isolates.
Kos
Veronica N
VN
Department of Medical Microbiology and Immunology, 1-41 Medical Sciences Building, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada.
Gibreel
Amera
A
Keelan
Monika
M
Taylor
Diane E
DE
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D017000:Campylobacter coli / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D017931:DNA Primers; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D004917:Erythromycin / Q000494:pharmacology*; D050296:Microbial Viability; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D004917:Erythromycin