title: Trimethylantimony dichloride causes genotoxic effects in Chinese hamster ovary cells after forced uptake.
abstract: In our study, we demonstrate that trimethylantimony dichloride (TMSb) does not induce micronucleus (MN) formation, chromosome aberrations (CA) or sister chromatid exchanges (SCE) under normal conditions in Chinese hamster ovary (CHO-9) cells in vitro up to an applied concentration of 1 mM, nor is it significantly cytotoxic. TMSb is taken up by the cells in a dose-dependent manner, but the percentage uptake of incubation substrate is low (max 0.05%). Intracellular TMSb concentration is two-fold increased after electroporation and under these forced uptake conditions MN formation is also significantly elevated. These data indicate that resistance to TMSb in CHO-9 cells occurs at the uptake and not at the intracellular level.
authors.affiliation: Institute of Hygiene and Occupational Medicine, University Hospital Essen, Hufelandstrasse 55, 45122 Essen, Germany. elke.dopp@uni-essen.de
authors.forename: E
authors.initials: E
authors.lastname: Dopp
authors.forename: L M
authors.initials: LM
authors.lastname: Hartmann
authors.forename: A-M
authors.initials: AM
authors.lastname: Florea
authors.forename: U
authors.initials: U
authors.lastname: von Recklinghausen
authors.forename: S
authors.initials: S
authors.lastname: Rabieh
authors.forename: B
authors.initials: B
authors.lastname: Shokouhi
authors.forename: A V
authors.initials: AV
authors.lastname: Hirner
authors.forename: A W
authors.initials: AW
authors.lastname: Rettenmeier
mesh_terms: D000818:Animals; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D018274:Electroporation; D048629:Micronuclei, Chromosome-Defective; D009942:Organometallic Compounds / Q000493:pharmacokinetics / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
chemical_list: D009942:Organometallic Compounds; C113588:trimethylantimony