title: Cell production in injured vocal folds: a rat study.
abstract: OBJECTIVE
  Fibroblasts are reported to play an important role in producing the extracellular matrix of the vocal fold. However, no reports have focused on how and where these cells are generated in the vocal fold after injury. To reveal the characteristics of vocal fold cell production, we investigated cell proliferation in the acute phase of wound healing.
  
  
  METHODS
  Using a telescope for guidance, we made an incision in the middle region of the vocal fold tissue in 24 rats and performed immunohistochemical staining for vimentin, alpha-smooth muscle actin, and 5-bromo-2-deoxyuridine.
  
  
  RESULTS
  After injury, epithelialization occurred with a peak at day 1, and fibroblasts proliferated in the lamina propria with a peak at day 3, whereas those in the macula flava did not show any increased proliferation.
  
  
  CONCLUSIONS
  It is suggested that the fibroblasts in the macula flava have functions different from those of fibroblasts in the lamina propria and that the macula flava does not serve as a cell source for the vocal fold in response to injury.
authors.affiliation: Division of Otolaryngology-Head and Neck Surgery, University of Wisconsin-Madison, K4-789 CSC, 600 Highland Ave, Madison, WI 53792, USA.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tateya
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tateya
authors.forename: Xinhong
authors.initials: X
authors.lastname: Lim
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Sohn
authors.forename: Diane M
authors.initials: DM
authors.lastname: Bless
mesh_terms: D000199:Actins; D000818:Animals; D001973:Bromodeoxyuridine; D049109:Cell Proliferation; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D007150:Immunohistochemistry; D007820:Laryngeal Mucosa / Q000166:cytology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014746:Vimentin; D014827:Vocal Cords / Q000166:cytology* / Q000293:injuries*; D014945:Wound Healing / Q000502:physiology
chemical_list: D000199:Actins; D016326:Extracellular Matrix Proteins; D014746:Vimentin; D001973:Bromodeoxyuridine