Microcantilevers modified by horseradish peroxidase intercalated nano-assembly for hydrogen peroxide detection.
Detection of hydrogen peroxide is of industry interest and of biological importance. Here we report a new approach to hydrogen peroxide measurement using multilayer modified microcantilevers. Through a layer-by-layer nanoassembly technique, horseradish peroxidase was intercalated into a nanoscale multilayer assembly on one surface of microcantilevers. These enzyme-functionalized microcantilevers deflected in response to hydrogen peroxide concentrations in the nanomolar level. The magnitudes of bending were proportional to the concentrations of hydrogen peroxide. Furthermore, our study also showed that microcantilever technique may be used as a novel, more sensitive tool for the study of the conformational or structural changes of enzymes or proteins on surfaces.
Yan
Xiaodong
X
Institute for Micromanufacturing, Louisiana Tech University, 911 Hergot Avenue, Ruston, LA 71272, USA.
Shi
Xiaolei
X
Hill
Kalisha
K
Ji
Hai-Feng
HF
D000327:Adsorption; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004798:Enzymes / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D008956:Models, Chemical; D008968:Molecular Conformation; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
D004798:Enzymes; D011506:Proteins; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase