Proposal of Giesbergeria voronezhensis gen. nov., sp. nov. and G. kuznetsovii sp. nov. and reclassification of [Aquaspirillum] anulus, [A.] sinuosum and [A.] giesbergeri as Giesbergeria anulus comb. nov., G. sinuosa comb. nov. and G. giesbergeri comb. nov., and [Aquaspirillum] metamorphum and [A.] psychrophilum as Simplicispira metamorpha gen. nov., comb. nov. and S. psychrophila comb. nov.
Five Gram-negative, motile, spiral-shaped strains were isolated from a sulfide spring (D-412T), active sludge of wastewater (D-419T, D-420, D-424) and industrial wastewater (D-416). Comparative 16S rRNA gene sequence analysis showed that the isolates belong to the family Comamonadaceae, within the class Betaproteobacteria, but fall into a distinct cluster. On the basis of phenotypic, chemotaxonomic and phylogenetic data, a new genus, Giesbergeria gen. nov., is proposed, including five species. The type species of the genus is Giesbergeria voronezhensis sp. nov. (type strain D-419T = DSM 12825T = CIP 107340T = VKM B-2350T) and other novel members of the genus are Giesbergeria kuznetsovii sp. nov. (type strain D-412T = DSM 12827T = VKM B-2352T), Giesbergeria giesbergeri comb. nov. (basonym Aquaspirillum giesbergeri), Giesbergeria sinuosa comb. nov. (basonym Aquaspirillum sinuosum) and Giesbergeria anulus comb. nov. (basonym Aquaspirillum anulus). Using the same criteria, isolate D-416 (= DSM 12826) was identified as a strain of [Aquaspirillum] metamorphum. Strain D-416, the type strain of [A.] metamorphum and the type strain of [Aquaspirillum] psychrophilum form a distinct cluster within the family Comamonadaceae (97-97.2% 16S rRNA gene sequence similarity) and share phenotypic and chemotaxonomic properties. Therefore, it is proposed that these strains are reclassified as members of a new genus, Simplicispira gen. nov., as Simplicispira metamorpha comb. nov. (the type species) and Simplicispira psychrophila comb. nov., respectively.
Grabovich
Margarita
M
Department of Biochemistry and Plant Physiology, Faculty of Biology, Voronezh State University, Voronezh, Russia.
Gavrish
Ekaterina
E
All Russian Collection of Microorganisms (VKM), G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
Kuever
Jan
J
Max-Planck-Institute for Marine Microbiology, Celsiusstraße 1, D-28359 Bremen, Germany.
Lysenko
Anatoly M
AM
S. N. Winogradsky Institute of Microbiology of the Russian Academy of Sciences, Pr. 60-letiya Octyabrya 7/2, Moscow, 117811, Russia.
Podkopaeva
Daria
D
Department of Biochemistry and Plant Physiology, Faculty of Biology, Voronezh State University, Voronezh, Russia.
Dubinina
Galina
G
S. N. Winogradsky Institute of Microbiology of the Russian Academy of Sciences, Pr. 60-letiya Octyabrya 7/2, Moscow, 117811, Russia.
D042621:Comamonadaceae / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000502:physiology; D004275:DNA, Ribosomal / Q000737:chemistry; D007220:Industrial Waste; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D004275:DNA, Ribosomal; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D012722:Sewage