Quantitative multiplex CARS spectroscopy in congested spectral regions.
A novel procedure is developed to describe and reproduce experimental coherent anti-Stokes Raman scattering (CARS) data, with particular emphasis on highly congested spectral regions. The approach, exemplified here with high-quality multiplex CARS data, makes use of spontaneous Raman scattering results. It is shown that the underlying vibrational Raman response can be retrieved from the multiplex CARS spectra, so that the Raman spectrum can be reconstituted, provided an adequate signal-to-noise ratio (SNR) is present in the experimental data and sufficient a priori knowledge of the vibrational resonances involved exists. The conversion of CARS to Raman data permits a quantitative interpretation of CARS spectra. This novel approach is demonstrated for highly congested multiplex CARS spectra of adenosine mono-, di-, and triphosphate (AMP, ADP, and ATP), nicotinamide adenine dinucleotide (NAD+), and small unilamellar vesicles (SUVs) of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). Quantitative determination of nucleotide concentrations and composition analysis in mixtures is demonstrated.
Rinia
Hilde A
HA
Swammerdam Institute for Life Sciences, University of Amsterdam, P.O. Box 94062, 1090 GB Amsterdam, The Netherlands.
Bonn
Mischa
M
Müller
Michiel
M
D000255:Adenosine Triphosphate / Q000737:chemistry; D009243:NAD / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014732:Vibration; D014867:Water / Q000737:chemistry
D010713:Phosphatidylcholines; D014867:Water; D009243:NAD; D000255:Adenosine Triphosphate; C028694:1-palmitoyl-2-oleoylphosphatidylcholine