Lipopolysaccharide heterogeneity among Burkholderia pseudomallei from different geographic and clinical origins.
Heterogeneous patterns were obtained for lipopolysaccharide (LPS) from 1,327 Burkholderia pseudomallei isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, silver staining, and immunoblot analysis. Two LPS serotypes (A and B) possessing different ladder profiles and a rough LPS without ladder appearances were identified. All three LPS types were antigenically distinct by immunoblotting. The predominant type A (97%) produced the lowest amount of biofilm. The two less common types (smooth type B and rough type) were found more in clinical than environmental isolates and more in Australian isolates than Thai isolates. These isolates were more often associated with relapse than with primary infection.
Anuntagool
Narisara
N
Faculty of Tropical Medicine, and Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand. narisara@tropmedres.ac
Wuthiekanun
Vanaporn
V
White
Nicholas J
NJ
Currie
Bart J
BJ
Sermswan
Rasana W
RW
Wongratanacheewin
Surasakdi
S
Taweechaisupapong
Suwimol
S
Chaiyaroj
Sansanee C
SC
Sirisinha
Stitaya
S
D000818:Animals; D000940:Antigenic Variation; D018441:Biofilms / Q000254:growth & development; D016957:Burkholderia pseudomallei / Q000378:metabolism* / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000737:chemistry / Q000145:classification* / Q000276:immunology / Q000378:metabolism; D008554:Melioidosis / Q000382:microbiology*
D008070:Lipopolysaccharides