Post-initiation chemopreventive effects of dietary bovine lactoferrin on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumorigenesis in female A/J mice.
We investigated the effects of bovine LF (bLF) on different phases of NNK-induced lung tumorigenesis in A/J mice. Mice were orally administered 0.02, 0.2 and 2% bLF during the initiation phase, and 2% bLF during the whole tumorigenesis phase or post-initiation phase. Administered bLF during the post-initiation phase showed significant reduction of macroscopical lung nodules, and immunohistochemically decreased expression levels of cell proliferation marker and increased expression levels of apoptosis marker in lung proliferative lesions. bLF might inhibit NNK-induced mouse lung tumorigenesis, only when given limited to the post-initiation phase, through modification of cell proliferation and/or apoptosis.
Matsuda
Yoko
Y
Department of Pathology and Host-Defense, Faculty of Medicine, Onco-Pathology, Kagawa University, 1750-1 Ikenobe, Kida-Gun, Kagawa, Japan.
Saoo
Kousuke
K
Hosokawa
Kyoko
K
Yamakawa
Keiko
K
Yokohira
Masanao
M
Zeng
Yu
Y
Takeuchi
Hijiri
H
Imaida
Katsumi
K
D000236:Adenoma / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D053148:Caspase 3 / Q000032:analysis; D002417:Cattle; D019587:Dietary Supplements; D005260:Female; D006965:Hyperplasia / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D007781:Lactoferrin / Q000008:administration & dosage / Q000627:therapeutic use*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D051379:Mice; D008805:Mice, Inbred A; D009602:Nitrosamines; D009929:Organ Size / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis
D009602:Nitrosamines; D018809:Proliferating Cell Nuclear Antigen; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone; D007781:Lactoferrin; D053148:Caspase 3