title: Lasers-an effective artificial source of radiation for the cultivation of anoxygenic photosynthetic bacteria.
abstract: The laser diode (LD) is a unique light source that can efficiently produce all radiant energy within the narrow wavelength range used most effectively by a photosynthetic microorganism. We have investigated the use of a single type of LD for the cultivation of the well-studied anoxygenic photosynthetic bacterium, Rhodobacter capsulatus (Rb. capsulatus). An array of vertical-cavity surface-emitting lasers (VCSELs) was driven with a current of 25 mA, and delivered radiation at 860 nm with 0.4 nm linewidth. The emitted light was found to be a suitable source of radiant energy for the cultivation of Rb. capsulatus. The dependence of growth rate on incident irradiance was quantified. Despite the unusual nearly monochromatic light source used in these experiments, no significant changes in the pigment composition and in the distribution of bacteriochlorophyll between LHII and LHI-RC were detected in bacterial cells transferred from incandescent light to laser light. We were also able to show that to achieve a given growth rate in a light-limited culture, the VCSEL required only 30% of the electricity needed by an incandescent bulb, which is of great significance for the potential use of laser-devices in biotechnological applications and photobioreactor construction.
authors.affiliation: School of Information Technology & Electrical Engineering, The University of Queensland, Brisbane Qld 4072, Australia.
authors.forename: K
authors.initials: K
authors.lastname: Bertling
authors.forename: T J
authors.initials: TJ
authors.lastname: Hurse
authors.forename: U
authors.initials: U
authors.lastname: Kappler
authors.forename: A D
authors.initials: AD
authors.lastname: Rakić
mesh_terms: D000222:Adaptation, Physiological; D007834:Lasers; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000528:radiation effects*; D045332:Photosystem II Protein Complex / Q000528:radiation effects*; D016354:Rhodobacter capsulatus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex