[Construction and expression of fusion gene encoding Hyper-IL-6 in mammalian cells].
OBJECTIVE
To construct and express fusion gene encoding Hyper-IL-6 in mammalian cells.


METHODS
Using geneSOEing method, human sIL-6R cDNA was fused with IL-6 cDNA by a linker rich in glycine through PCR technique and cloned into pIRES(2)-EGFP. The constructed plasmid was transfected into mammalian cells. The expression of fusion gene encoding Hyper-IL-6 was detected by GFP and RT-PCR and immunocytochemistry.


RESULTS
The recombinant plasmid pIRES-HIL-6 was successfully constructed and expressed in mammalian cells.


CONCLUSIONS
The success in construction and expression of human Hyper-IL-6 makes it possible to further study its biological functions.
Qie
Chun-hua
CH
Viral Institute for Hepatitis, Key Laboratory of Molecular Biology on Infectious Diseases of Ministry of Education, Chongqing University of Medical Sciences, Chongqing 400010, China.
Qin
Bo
B
Huang
Ai-long
AL
Liu
Qi
Q
Chen
Guo-min
GM
D000818:Animals; D018076:DNA, Complementary; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D004274:DNA, Recombinant; D015850:Interleukin-6; D011993:Recombinant Fusion Proteins