title: Destruction of human retinoblastoma after treatment by the E variant of encephalomyocarditis virus.
abstract: The oncolytic effects of encephalomyocarditis (EMC) virus were examined in human retinoblastoma cell (Y79) cultures which were infected with 10(4 )tissue culture infectious doses (TCIDs) of the E variant of EMC (EMC-E) virus. The TCIDs were used to titer the maximum effect of EMC virus on L-929 cells. In-vitro studies showed 90% cytopathic effect (CPE) at 24 h and 100% CPE at 52 h. The CPE was used to observe pathologic effects of the cells. In-vivo studies employing human retinoblastoma grown as a tumor in nude mice, revealed degeneration of 80% of the tumor cells at 3 days and total destruction at 4 days following inoculation with the EMC-E virus. The virus is highly neurotropic in mice, but is usually not pathogenic in man. These studies suggest a possible new direction in the treatment of retinoblastoma and other malignant tumors using the viral technology.
authors.affiliation: Department of Laboratories, Isaac Albert Research Institute of Kingsbrook Jewish Medical Center, 585 Schenectady Ave., Brooklyn, NY 11203, USA.
authors.forename: Masazumi
authors.initials: M
authors.lastname: Adachi
authors.forename: Steven E
authors.initials: SE
authors.lastname: Brooks
authors.forename: Maxine R
authors.initials: MR
authors.lastname: Stein
authors.forename: Bernice E
authors.initials: BE
authors.lastname: Franklin
authors.forename: Francis A
authors.initials: FA
authors.lastname: Caccavo
mesh_terms: D000818:Animals; D004686:Encephalomyocarditis virus / Q000145:classification; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000473:pathology / Q000628:therapy* / Q000648:ultrastructure / Q000821:virology; D050130:Oncolytic Virotherapy / Q000379:methods*; D050504:Oncolytic Viruses / Q000145:classification; D012175:Retinoblastoma / Q000473:pathology / Q000628:therapy* / Q000648:ultrastructure / Q000821:virology*; D014407:Tumor Cells, Cultured