[Bioinformatics analysis of YZ-2 and preparation of polyclonal antibody].
OBJECTIVE
To analyze YZ-2, a novel protein associated with cerebral ischemia and prepares its polyclonal antibody.


METHODS
According to the bioinformatics analysis and prediction of the possible high structure, hydrophilicity and antigenicity of YZ-2, a 22-amino acid residue partial peptide of YZ-2 was synthesized. The synthesized peptide was then used to immunize. And the properties of anti-YZ2 were analyzed by ELISA and Western blot.


RESULTS
The hydrophilicity and antigenicity were predicted by methods of bioinformatics. The polyclonal antibody of YZ-2 was successfully obtained and its specificity and sensitivity were conformed by ELISA and Western blot.


CONCLUSIONS
By the bioinformatics analysis and prediction, the hydrophilicity and antigenicity of YZ2 were analyzed. The antibody of YZ-2 was successfully obtained.
Zang
Lin-quan
LQ
Department of Pharmacology, Pharmaceutical School, Guangdong Pharmaceutical University, Guangzhou 510006, China. zanglq@yahoo.com
Qiu
Peng-xin
PX
Xiao
Ru
R
Yan
Guang-mei
GM
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D019411:Clinical Laboratory Techniques; D019295:Computational Biology; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D011506:Proteins / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
D000906:Antibodies; D011506:Proteins