Metabonomic strategy for the investigation of the mode of action of the phytotoxin (5S,8R,13S,16R)-(-)-pyrenophorol using 1H nuclear magnetic resonance fingerprinting.
The biochemical mode of action of (5S,8R,13S,16R)-(-)-pyrenophorol isolated from a Drechslera avenae pathotype was investigated by using metabolic fingerprinting. (1)H NMR spectra of crude leaf extracts from untreated Avena sterilis seedlings and A. sterilis seedlings treated with pyrenophorol were compared with those obtained from treatments with the herbicides diuron, glyphosate, mesotrione, norflurazon, oxadiazon, and paraquat. Multivariate analysis was carried out to group treatments according to the mode of action of the phytotoxic substances applied. Analysis results revealed that none of the herbicide treatments fitted the pyrenophorol model and indicate that the effect of the phytotoxin on A. sterilis differs than those caused by glyphosate, mesotrione, norflurazon, oxadiazon, paraquat, and diuron, which inhibit 5-enolpyruvylshikimate-3-phosphate synthase, 4-hydroxyphenyl-pyruvate-dioxygenase, phytoene desaturase, protoporphyrinogen oxidase, photosystem I, and photosystem II, respectively. The method applied, combined with appropriate data preprocessing and analysis, was found to be rapid for the screening of phytotoxic substances for metabolic effects.
Aliferis
Konstantinos A
KA
Pesticide Science Laboratory, Agricultural University of Athens, Greece.
Chrysayi-Tokousbalides
Maria
M
D000704:Analysis of Variance; D001203:Ascomycota / Q000737:chemistry; D006540:Herbicides / Q000494:pharmacology*; D006571:Heterocyclic Compounds / Q000494:pharmacology*; D007659:Ketones / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D006109:Poaceae / Q000737:chemistry / Q000187:drug effects; D036226:Seedlings / Q000737:chemistry
D006540:Herbicides; D006571:Heterocyclic Compounds; D007659:Ketones; D010936:Plant Extracts; C509954:pyrenophorol