[Study on mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP].
The polymorphism of mtDNA D-loop of 83 individuals from 9 Chinese indigenous sheep breeds and 2 imported sheep breeds were studied with 5 endonucleases, Hinf I, Msp I, Sau3A I, Xsp I and Taq I, using PCR-RFLP. The results indicated that there existed two basic haplotypes in the region of mtDNA D-loop. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors. The averaged polymorphic degree (Pi value=0.0421%) of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very poor.
Li
Xiang-Long
XL
Department of Animal Science and Technology, Hebei Agriculture University, Baoding 071001, China. lixianglongcn@yahoo.com
Zhang
Zeng-Li
ZL
Gong
Yuan-Fang
YF
Liu
Zheng-Zhu
ZZ
Jia
Qing
Q
Wang
Li-Ze
LZ
D000818:Animals; D002681:China; D006239:Haplotypes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D012756:Sheep / Q000235:genetics*