Transformation of rhizobia with broad-host-range plasmids by using a freeze-thaw method.
Several species of rhizobia were successfully transformed with broad-host-range plasmids of different replicons by using a modified freeze-thaw method. A generic binary vector (pPZP211) was maintained in Mesorhizobium loti without selection and stably inherited during nodulation. The method could extend the potential of rhizobia as a vehicle for plant transformation.
Vincze
Eva
E
Department of Genetics and Biotechnology, Research Centre Flakkebjerg, Danish Institute of Agricultural Sciences, Forsøgsvej 1, DK-4200 Slagelse, Denmark. eva.vincze@agrsci.dk
Bowra
Steve
S
D020561:Alphaproteobacteria / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D020369:Bradyrhizobium / Q000235:genetics; D003470:Culture Media; D005615:Freezing; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics; D020579:Sinorhizobium / Q000235:genetics; D014169:Transformation, Bacterial
D003470:Culture Media