[The application of microsatellite markers for parentage determination in selective breeding of Pacific white shrimp (Litopenaeus vannamei)].
Used simulations and controlled mating to examine the potential of microsatellite markers in assigning parentage to Pacific white shrimp progeny. Cervus simulations demonstrated that the theoretical expectations for parentage exclusion of 10 microsatellite loci and six most polymorphic of the 10 loci were both 0.99, and the assignment success rate of the 6 most polymorphic microsatellite loci set was nearly to 0.97 with 95% confidence. Based on this information, offspring from 10 crosses where parents were known were genotyped by the 6 microsatellite loci and used for parentage analysis. The result showed that assignment success of the progeny to their 'true' mother and father was 88% and 78% respectively, which were lower than predicted by the Cervus simulations. This could be explained by the existence of null or mutant alleles and by Taq DNA Polymerase slippage in the microsatellite loci.
Wang
Hong-Xia
HX
Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. hongxiawang007@ms.qdio.ac.cn
Wu
Chang-Gong
CG
Zhang
Liu-Suo
LS
Xiang
Jian-Hai
JH
D000818:Animals; D018895:Microsatellite Repeats / Q000235:genetics*; D010375:Pedigree; D033561:Penaeidae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction