Inhibitory effect of heparan sulfate-like glycosaminoglycans on the infectivity of Chlamydia pneumoniae in HL cells varies between strains.
Glycosaminoglycans are known to participate in the attachment of several chlamydial strains. We studied the effect of heparin, enoxaparin, low-molecular-weight heparin, chondroitin sulfate A, and heparinase I on the infectivity of Chlamydia pneumoniae strain CWL029 and two Finnish isolates, Kajaani 7 and Parola, in an HL cell line which is epithelial in origin. Two Chlamydia trachomatis strains, L2 and E, were used for comparison. The infectivity of all C. pneumoniae strains and C. trachomatis serovar E was inhibited not only by heparin derivatives but also by chondroitin sulfate A and heparinase treatment. Treatment of host cells with heparin derivatives and heparinase was also inhibitory. Different chlamydial strains and species seem, however, to vary in their ability to use heparin in their attachment to host cells.
Yan
Ying
Y
Department of Medical Microbiology, University of Oulu, P.O. Box 5000, 90014 Oulu, Finland. ying.yan@oulu.fi
Silvennoinen-Kassinen
Sylvi
S
Leinonen
Maija
M
Saikku
Pekka
P
D000818:Animals; D002460:Cell Line; D002692:Chlamydia trachomatis / Q000378:metabolism; D016993:Chlamydophila pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology*; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006801:Humans; D051379:Mice
D006025:Glycosaminoglycans