title: Structure and binding kinetics of three different human CD1d-alpha-galactosylceramide-specific T cell receptors.
abstract: Invariant human TCR Valpha24-Jalpha18+/Vbeta11+ NKT cells (iNKT) are restricted by CD1d-alpha-glycosylceramides. We analyzed crystal structures and binding characteristics for an iNKT TCR plus two CD1d-alpha-GalCer-specific Vbeta11+ TCRs that use different TCR Valpha chains. The results were similar to those previously reported for MHC-peptide-specific TCRs, illustrating the versatility of the TCR platform. Docking TCR and CD1d-alpha-GalCer structures provided plausible insights into their interaction. The model supports a diagonal orientation of TCR on CD1d and suggests that complementarity determining region (CDR)3alpha, CDR3beta, and CDR1beta interact with ligands presented by CD1d, whereas CDR2beta binds to the CD1d alpha1 helix. This docking provides an explanation for the dominant usage of Vbeta11 and Vbeta8.2 chains by human and mouse iNKT cells, respectively, for recognition of CD1d-alpha-GalCer.
authors.affiliation: Department of Rheumathology and Clinical Immunology, University of Bern, Inselspital, Berne CH-3010, Switzerland. stephan.gadola@insel.ch
authors.forename: Stephan D
authors.initials: SD
authors.lastname: Gadola
authors.forename: Michael
authors.initials: M
authors.lastname: Koch
authors.forename: Jon
authors.initials: J
authors.lastname: Marles-Wright
authors.forename: Nikolai M
authors.initials: NM
authors.lastname: Lissin
authors.forename: Dawn
authors.initials: D
authors.lastname: Shepherd
authors.forename: Gediminas
authors.initials: G
authors.lastname: Matulis
authors.forename: Karl
authors.initials: K
authors.lastname: Harlos
authors.forename: Peter M
authors.initials: PM
authors.lastname: Villiger
authors.forename: David I
authors.initials: DI
authors.lastname: Stuart
authors.forename: Bent K
authors.initials: BK
authors.lastname: Jakobsen
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Cerundolo
authors.forename: E Yvonne
authors.initials: EY
authors.lastname: Jones
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D018949:Antigens, CD1 / Q000737:chemistry* / Q000276:immunology; D055610:Antigens, CD1d; D022801:Complementarity Determining Regions / Q000737:chemistry* / Q000276:immunology; D018360:Crystallography, X-Ray; D005699:Galactosylceramides / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008958:Models, Molecular; D011485:Protein Binding / Q000276:immunology; D020836:Protein Structure, Quaternary; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry* / Q000276:immunology; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D018949:Antigens, CD1; D055610:Antigens, CD1d; C526877:CD1D protein, human; D022801:Complementarity Determining Regions; D005699:Galactosylceramides; D016693:Receptors, Antigen, T-Cell, alpha-beta