Application of liposomes to sonoporation.
A method to prepare liposomes is presented. Liposomes made in our laboratory were characterized acoustically and optically. The phase velocity and attenuation of liposomes in suspension (concentration = 10(9)/mL) were measured, ranging from 2 to 14 MHz, using ultrasound spectroscopy. Anti-rabbit IgG conjugated with Alexafluor 647 was delivered into Jurkat cells in suspension, using the liposomes, by 10 % duty cycle ultrasound tonebursts of 2.2 MHz (the in situ spatial peak-pressure amplitude = 80 W/cm2) with an efficiency of 13 %. It has been experimentally shown that liposomes may be an alternative stable agent to Optison for delivering macromolecules into cells.
Wu
Junru
J
Department of Physics, University of Vermont, Burlington, VT 05405-0125, USA. JWU@ZOO.UVM.EDU
Chen
Di
D
Pepe
Jason
J
Himberg
Benjamin E
BE
Ricń
Mercedes
M
D000418:Albumins; D000818:Animals; D002138:Calibration; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D005466:Fluorocarbons; D006801:Humans; D007074:Immunoglobulin G / Q000008:administration & dosage; D019169:Jurkat Cells; D008081:Liposomes; D045423:Microbubbles; D015612:Phonophoresis / Q000379:methods*; D011817:Rabbits; D013057:Spectrum Analysis / Q000379:methods; D013601:T-Lymphocytes / Q000378:metabolism*
D000418:Albumins; C099458:FS 069; D005466:Fluorocarbons; D007074:Immunoglobulin G; D008081:Liposomes