Development of a process for the production of the anticancer lead compound pleurotin by fermentation of Hohenbuehelia atrocaerulea.
Pleurotin is a naphthoquinone antibiotic originally isolated from Pleurotus griseus. Two pleurotin producing strains of Hohenbuehelia atrocaerulea have been identified, which, on solid substrate fermentation for 2 months yield 1-2 mg/l of the antibiotic. Described here is the lengthy developmental process which resulted in a production protocol being developed which reliably yields pleurotin from liquid fermentation at >300 mg/l. Critical to obtaining this increase in titer was inclusion in the media of an aqueous extract of alder wood.
Shipley
S M
SM
Natural Products Support Group, SAIC-Frederick, Inc, Bldg. 431, Box B, Frederick, MD 21702, USA.
Barr
A L
AL
Graf
S J
SJ
Collins
R P
RP
McCloud
T G
TG
Newman
D J
DJ
D000363:Agaricales / Q000378:metabolism*; D029661:Alnus; D005285:Fermentation; D006576:Heterocyclic Compounds, 4 or More Rings / Q000378:metabolism*; D015394:Molecular Structure; D010936:Plant Extracts / Q000378:metabolism
D006576:Heterocyclic Compounds, 4 or More Rings; D010936:Plant Extracts; C479485:pleurotin