title: A family knockout of all four Drosophila metallothioneins reveals a central role in copper homeostasis and detoxification.
abstract: Metallothioneins are ubiquitous, small, cysteine-rich proteins with the ability to bind heavy metals. In spite of their biochemical characterization, their in vivo function remains elusive. Here, we report the generation of a metallothionein gene family knockout in Drosophila melanogaster by targeted disruption of all four genes (MtnA to -D). These flies are viable if raised in standard laboratory food. During development, however, they are highly sensitive to copper, cadmium, and (to a lesser extent) zinc load. Metallothionein expression is particularly important for male viability; while copper load during development affects males and females equally, adult males lacking metallothioneins display a severely reduced life span, possibly due to copper-mediated oxidative stress. Using various reporter gene constructs, we find that different metallothioneins are expressed with virtually the same tissue specificity in larvae, notably in the intestinal tract at sites of metal accumulation, including the midgut's "copper cells." The same expression pattern is observed with a synthetic minipromoter consisting only of four tandem metal response elements. From these and other experiments, we conclude that tissue specificity of metallothionein expression is a consequence, rather than a cause, of metal distribution in the organism. The bright orange luminescence of copper accumulated in copper cells of the midgut is severely reduced in the metallothionein gene family knockout, as well as in mutants of metal-responsive transcription factor 1 (MTF-1), the main regulator of metallothionein expression. This indicates that an in vivo metallothionein-copper complex forms the basis of this luminescence. Strikingly, metallothionein mutants show an increased, MTF-1-dependent induction of metallothionein promoters in response to copper, cadmium, silver, zinc, and mercury. We conclude that free metal, but not metallothionein-bound metal, triggers the activation of MTF-1 and that metallothioneins regulate their own expression by a negative feedback loop.
authors.affiliation: IMB Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
authors.forename: Dieter
authors.initials: D
authors.lastname: Egli
authors.forename: Hasmik
authors.initials: H
authors.lastname: Yepiskoposyan
authors.forename: Anand
authors.initials: A
authors.lastname: Selvaraj
authors.forename: Kuppusamy
authors.initials: K
authors.lastname: Balamurugan
authors.forename: Rama
authors.initials: R
authors.lastname: Rajaram
authors.forename: Andreas
authors.initials: A
authors.lastname: Simons
authors.forename: Gerd
authors.initials: G
authors.lastname: Multhaup
authors.forename: Simone
authors.initials: S
authors.lastname: Mettler
authors.forename: Alla
authors.initials: A
authors.lastname: Vardanyan
authors.forename: Oleg
authors.initials: O
authors.lastname: Georgiev
authors.forename: Walter
authors.initials: W
authors.lastname: Schaffner
mesh_terms: D000375:Aging / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D002104:Cadmium / Q000378:metabolism; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006706:Homeostasis / Q000235:genetics*; D008658:Inactivation, Metabolic / Q000235:genetics*; D049449:Luminescence; D008297:Male; D008628:Mercury / Q000378:metabolism / Q000494:pharmacology; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism*; D005810:Multigene Family; D011995:Recombination, Genetic; D020218:Response Elements; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000097809:Transcription Factor MTF-1
chemical_list: D002104:Cadmium; D003300:Copper; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D008628:Mercury; D008668:Metallothionein; D014157:Transcription Factors; D000097809:Transcription Factor MTF-1