title: Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence.
abstract: OBJECTIVE
  We have previously shown that phototherapy increases cell growth and impairs protein secretion of fibroblasts. Our objective was to study the effect of phototherapy on osteoblast-like cells in culture treated with dexamethasone.
  
  
  METHODS
  Rat calvaria osteoblast-like cells were previously treated or not with dexamethasone and then, they were irradiated or not with a GaAlAs diode laser (wavelength of 780 nm, 10 mW, 3 J/cm2). Adhesion, proliferation, and osteonectin synthesis were analyzed.
  
  
  RESULTS
  Phototherapy increased the proliferation rate of cells independently of dexamethasone presence. Adhesion and osteonectin synthesis were not significantly influenced by laser and/or dexamethasone.
  
  
  CONCLUSIONS
  Based on the conditions of this study we concluded that phototherapy acts as a proliferative stimulus on osteoblast-like cells, even under the influence of dexamethasone. Thus, we suggest that phototherapy can be of importance as co-adjuvant in bone clinical manipulation in order to accelerate bone regeneration.
authors.affiliation: Department of Oral Pathology, São Paulo, São Paulo, Brazil 05508-900.
authors.forename: Neusa A
authors.initials: NA
authors.lastname: Fujihara
authors.forename: Karen R N
authors.initials: KR
authors.lastname: Hiraki
authors.forename: Márcia M
authors.initials: MM
authors.lastname: Marques
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000528:radiation effects; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D003907:Dexamethasone / Q000494:pharmacology; D028022:Low-Level Light Therapy; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects*; D015676:Osteonectin / Q000096:biosynthesis; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D015676:Osteonectin; D003907:Dexamethasone