title: Protein and gene expression of VTG in response to 4-nonylphenol in rockfish (Sebastes schlegeli).
abstract: To investigate the in vivo estrogenic activity of 4-nonylphenol (4-NP) on reproductive function, we have measured the expression level of rockfish vitellogenin (rVTG) mRNA, concentration of plasma VTG and levels of plasma estradiol-17beta(E(2)) and testosterone (T) by radioimmunoassay (RIA). An rVTG mRNA transcript of approximately 4.0 kb was extracted from hepatic tissue for Northern blot analysis, and the effects of 4-NP on rVTG mRNA expression in vivo with both male and female juvenile rockfish were examined. The level of rVTG mRNA expression was increased 48 h after injection with 4-NP of 10 mg/kg body mass and after injection in both male and female rockfish. The level of rVTG mRNA expression was increased 24 h after injection in male rockfish injected with 4-NP of 25 mg/kg body mass, while the level of rVTG mRNA expression was increased 12h after injection in female injected with same dosage. Concentrations of plasma rVTG in female injected with 4-NP of 10 and 25 mg/kg b.w. were increased 72 h after injection and reached 35 and 66 mg/mL, respectively. In male, rVTG concentrations with the dosage of 25 mg/kg body mass were increased 72 h after injection and reached to 14 and 65 mg/mL at 168 h, respectively. Plasma concentrations of T and E(2) in female and male injected with 4-NP were not significantly different between two 4-NP dosages. These results suggest that 4-NP may disrupt the reproductive system of immature rockfish by acting directly on vitellogenesis. Immature females injected with 4-NP were more sensitive than immature males, but VTG in immature males is likely to return to normal conditions slowly. There are obviously large interspecies differences in response of fish to 4-NP.
authors.affiliation: South Sea Institute, KORDI, 391 Jangbuk-ri Jangmok-myon, Geoje.
authors.forename: Jee-Hyun
authors.initials: JH
authors.lastname: Jung
authors.forename: Won Joon
authors.initials: WJ
authors.lastname: Shim
authors.forename: R F
authors.initials: RF
authors.lastname: Addison
authors.forename: Jae Min
authors.initials: JM
authors.lastname: Baek
authors.forename: Chang-Hee
authors.initials: CH
authors.lastname: Han
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000097:blood; D005260:Female; D005399:Fishes / Q000097:blood / Q000378:metabolism*; D005786:Gene Expression Regulation; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010636:Phenols / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D012737:Sex Factors; D013739:Testosterone / Q000097:blood; D013997:Time Factors; D014818:Vitellogenesis / Q000187:drug effects; D014819:Vitellogenins / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D010636:Phenols; D012333:RNA, Messenger; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D013739:Testosterone; D004958:Estradiol; C041594:4-nonylphenol