title: Crystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds.
abstract: A lectin from Canavalia maritima seeds (ConM) was purified and submitted to crystallization experiments. The best crystals were obtained using the vapour-diffusion method at a constant temperature of 293 K and grew in 7 d. A complete structural data set was collected to 2.1 A resolution using a synchrotron-radiation source. The ConM crystal belongs to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 67.15, b = 70.90, c = 97.37 A. A molecular-replacement search found a solution with a correlation coefficient of 69.2% and an R factor of 42.5%. Crystallographic refinement is under way.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Caixa Postal 6043, CEP 60455-970, Brazil.
authors.forename: Carlos Alberto de Almeida
authors.initials: CA
authors.lastname: Gadelha
authors.forename: Frederico Bruno Mendes Batista
authors.initials: FB
authors.lastname: Moreno
authors.forename: Tatiane
authors.initials: T
authors.lastname: Santi-Gadelha
authors.forename: João Batista
authors.initials: JB
authors.lastname: Cajazeiras
authors.forename: Bruno Anderson M da
authors.initials: BA
authors.lastname: Rocha
authors.forename: Joane Kathelen Rodrigues
authors.initials: JK
authors.lastname: Rustiguel
authors.forename: Beatriz Tupinamba
authors.initials: BT
authors.lastname: Freitas
authors.forename: Fernanda
authors.initials: F
authors.lastname: Canduri
authors.forename: Plínio
authors.initials: P
authors.lastname: Delatorre
authors.forename: Walter Filgueira de
authors.initials: WF
authors.lastname: Azevedo
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D032449:Canavalia / Q000737:chemistry*; D003460:Crystallization; D037121:Plant Lectins / Q000737:chemistry* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D037121:Plant Lectins; C039268:lectin, Canavalia