What is the true supply of amino acids for a dairy cow?
Improving the prediction of milk protein yield relies on knowledge of both protein supply and requirement. Definition of protein/amino acid supply in ruminants is a challenging task, due to feedstuff variety and variability and to the remodeling of nutrient intake by the rumen microflora. The questions arise, therefore, how and where should we measure the real supply of AA in the dairy cow? This review will follow the downstream flow of AA from duodenum to peripheral tissue delivery, with a glance at the efficiency of transfer into milk protein. Duodenal AA flow comprises rumen undegradable feed, microbial protein, and endogenous secretions. Most attention has been directed toward definition of the first two contributions but the latter fraction can represent as much as 20% of duodenal flow. More information is needed on what factors affect its magnitude and overall impact. Once digested, AA are absorbed into the portal vein. The ratio of portal absorption to small intestinal apparent digestion varies among essential AA, from 0.43 (threonine) to 0.76 (phenylalanine), due to the contributions of preduodenal endogenous secretions to the digestive flow, non-reabsorption of endogenous secretions and gut oxidation of AA. Few data are available on these phenomena in dairy cows but the evidence indicates that they alter the profile of AA available for anabolic purposes. Recent comparisons of estimated duodenal flux and measured portal flux have prompted a revisit of the NRC (2001) approach to estimate AA flows at the duodenum. Changes to the model are proposed that yield predictions that better fit the current knowledge of AA metabolism across the gut. After absorption, AA flow first to the liver where substantial and differential net removal occurs, varying from zero for the branched-chain AA to 50% of portal absorption for phenylalanine. This process alters the pattern of net supply to the mammary gland. Overall, intermediary metabolism of AA between the duodenum and the mammary gland biologically explains the decreased efficiency of the transfer of absorbed AA into milk protein as maximal yield is approached. Therefore, variable, rather than fixed, factors for transfer efficiencies must be incorporated into future predictive models.
Lapierre
H
H
Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Lennoxville, Quebec, J1M 1Z3, Canada. lapierreh@agr.gc.ca
Pacheco
D
D
Berthiaume
R
R
Ouellet
D R
DR
Schwab
C G
CG
Dubreuil
P
P
Holtrop
G
G
Lobley
G E
GE
D000042:Absorption; D000601:Amino Acids, Essential / Q000032:analysis / Q000378:metabolism* / Q000493:pharmacokinetics; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001682:Biological Availability; D002417:Cattle / Q000378:metabolism / Q000502:physiology*; D003612:Dairying; D004044:Dietary Proteins / Q000378:metabolism / Q000493:pharmacokinetics; D004386:Duodenum / Q000378:metabolism; D005260:Female; D007419:Intestinal Secretions / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D008954:Models, Biological; D010084:Oxidation-Reduction; D011169:Portal Vein / Q000378:metabolism
D000601:Amino Acids, Essential; D004044:Dietary Proteins