title: Purification, crystallization and preliminary X-ray diffraction analysis of the Kelch-like motif region of mouse Keap1.
abstract: Keap1 (Kelch-like ECH-associating protein 1) is a negative regulator of the Nrf2 transcription factor in the cytoplasm. The Kelch/DGR (double-glycine repeat) domain of Keap1 associates with Nrf2 as well as with actin filaments. A recombinant protein containing both the Kelch/DGR domain and the C-terminal region of mouse Keap1 was expressed in Escherichia coli, purified to near-homogeneity and crystallized by the sitting-drop vapour-diffusion method. The crystal belongs to space group P6(1) or P6(5), with unit-cell parameters a = b = 102.95, c = 55.21 A, and contains one molecule in the asymmetric unit. A complete diffraction data was collected to 2.25 A resolution using an R-AXIS IV++ imaging plate mounted on an RA-Micro7 Cu Kalpha rotating-anode X-ray generator.
authors.affiliation: RIKEN Genomic Sciences Center, Tsurumi, Yokohama 230-0045, Japan.
authors.forename: Balasundaram
authors.initials: B
authors.lastname: Padmanabhan
authors.forename: Maria
authors.initials: M
authors.lastname: Scharlock
authors.forename: Kit I
authors.initials: KI
authors.lastname: Tong
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Moon-Il
authors.initials: MI
authors.lastname: Kang
authors.forename: Akira
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Takehisa
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Akiko
authors.initials: A
authors.lastname: Tanaka
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D000199:Actins / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D003001:Cloning, Molecular; D003460:Crystallization; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli; D000072019:Kelch-Like ECH-Associated Protein 1; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C117301:Keap1 protein, mouse; D000072019:Kelch-Like ECH-Associated Protein 1; D011993:Recombinant Fusion Proteins