Crystallization and preliminary X-ray diffraction analysis of HML, a lectin from the red marine alga Hypnea musciformis.
HML, a lectin from the red marine alga Hypnea musciformis, defines a novel lectin family. Orthorhombic crystals of HML belonging to space group P2(1)2(1)2(1) grew within three weeks at 293 K using the hanging-drop vapour-diffusion method. A complete data set was collected at 2.4 A resolution. HML is the first marine alga lectin to be crystallized.
Nagano
Celso S
CS
Instituto de Biomedicina de Valencia, CSIC, Valencia, Spain.
Gallego del Sol
Francisca
F
Cavada
Benildo S
BS
Nascimento
Kyria Santiago Do
KS
Nunes
Eudismar Vale
EV
Sampaio
Alexandre H
AH
Calvete
Juan J
JJ
D020418:Algal Proteins / Q000737:chemistry*; D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004058:Diffusion; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry*; D015233:Models, Statistical; D008969:Molecular Sequence Data; D000461:Rhodophyta / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D014461:Ultracentrifugation; D014961:X-Ray Diffraction
D020418:Algal Proteins; D037102:Lectins