title: Analysis of cytotoxic T lymphocyte associated antigen 4 gene polymorphisms in patients with ulcerative colitis.
abstract: OBJECTIVE
  Ulcerative colitis (UC) is a multifactorial disease associated with dysregulated immunity. Recently, cytotoxic T lymphocyte associated antigen 4 (CTLA-4) gene polymorphisms have been reported in association with several autoimmune diseases in several populations. In the present study, the possible implication of the CTLA-4 gene as a risk factor for UC in the Iranian population was investigated.
  
  
  METHODS
  One hundred UC patients and 100 healthy subjects were studied. CTLA-4 exon 1 position 49 (A/G: codon 17: Thr/Ala) polymorphisms were investigated by polymerase chain reaction single strand confirmation polymorphism method. Four of the patients and one of the healthy controls were excluded from the study because of incomplete DNA extraction.
  
  
  RESULTS
  The allele frequencies of A and G in 96 patients (A: 66.1%; G: 33.9%) were not significantly different from the 99 control subjects (A: 63.1%; G: 36.9%, P > 0.05). No significant differences in the distribution of genotype frequencies were observed between A + 49G gene polymorphisms and UC in the Iranian population (P > 0.05).
  
  
  CONCLUSIONS
  CTLA-4 polymorphism is not associated with UC in the Iranian population.
authors.affiliation: Gastroenterohepatology Research Center, Nemazee Hospital, Shiraz, Iran. lankaran@sums.ac.ir
authors.forename: Kamran B
authors.initials: KB
authors.lastname: Lankarani
authors.forename: Ashraf
authors.initials: A
authors.lastname: Karbasi
authors.forename: Tahereh
authors.initials: T
authors.lastname: Kalantari
authors.forename: Hooman
authors.initials: H
authors.lastname: Yarmohammadi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Saberi-Firoozi
authors.forename: Mahvash
authors.initials: M
authors.lastname: Alizadeh-Naeeni
authors.forename: Ali R
authors.initials: AR
authors.lastname: Taghavi
authors.forename: Mahammad R
authors.initials: MR
authors.lastname: Fattahi
authors.forename: Abbas
authors.initials: A
authors.lastname: Ghaderi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000235:genetics*; D060908:CTLA-4 Antigen; D003093:Colitis, Ulcerative / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D004247:DNA / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D015994:Incidence; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D004247:DNA