Expression of ethylene receptors Dl-ERS1-3 and Dl-ERS2, and ethylene response during flower senescence in Delphinium.
To clarify the relationships of flower senescence, especially sepal abscission, and ethylene receptor gene expression in different flower parts, we isolated two cDNAs encoding ethylene receptors Dl-ERS1-3 and Dl-ERS2 from Delphinium flowers. Deduced polypeptides possessed no response regulator domain, indicating that they belong to a family of ethylene response sensor (ERS) ethylene receptors. Dl-ERS1-3 and Dl-ERS2 exhibited constitutive levels during flower senescence. Exogenous ethylene increased transcript levels in sepals, which are influenced by ethylene but not in gynoecia and receptacles, which produce ethylene. It was suggested that expression of ethylene receptor genes under ethylene exposure was differentially regulated in each organ of the flower.
Tanase
Koji
K
National Institute of Floricultural Science, National Agriculture and Bio-oriented Research Organization, Fujimoto 2-1, Tsukuba 305-8519, Ibaraki, Japan. tanase@affrc.go.jp
Ichimura
Kazuo
K
D000595:Amino Acid Sequence; D017124:Conserved Sequence; D031882:Delphinium / Q000145:classification / Q000254:growth & development* / Q000502:physiology*; D005030:Ethylenes / Q000378:metabolism / Q000494:pharmacology*; D035264:Flowers / Q000254:growth & development / Q000502:physiology*; D015870:Gene Expression; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D005030:Ethylenes; D010940:Plant Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant; C036216:ethylene