Trimethylantimony dichloride causes genotoxic effects in Chinese hamster ovary cells after forced uptake.
In our study, we demonstrate that trimethylantimony dichloride (TMSb) does not induce micronucleus (MN) formation, chromosome aberrations (CA) or sister chromatid exchanges (SCE) under normal conditions in Chinese hamster ovary (CHO-9) cells in vitro up to an applied concentration of 1 mM, nor is it significantly cytotoxic. TMSb is taken up by the cells in a dose-dependent manner, but the percentage uptake of incubation substrate is low (max 0.05%). Intracellular TMSb concentration is two-fold increased after electroporation and under these forced uptake conditions MN formation is also significantly elevated. These data indicate that resistance to TMSb in CHO-9 cells occurs at the uptake and not at the intracellular level.
Dopp
E
E
Institute of Hygiene and Occupational Medicine, University Hospital Essen, Hufelandstrasse 55, 45122 Essen, Germany. elke.dopp@uni-essen.de
Hartmann
L M
LM
Florea
A-M
AM
von Recklinghausen
U
U
Rabieh
S
S
Shokouhi
B
B
Hirner
A V
AV
Rettenmeier
A W
AW
D000818:Animals; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D018274:Electroporation; D048629:Micronuclei, Chromosome-Defective; D009942:Organometallic Compounds / Q000493:pharmacokinetics / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D009942:Organometallic Compounds; C113588:trimethylantimony