title: The structure at 2.4 A resolution of the protein from gene locus At3g21360, a putative Fe(II)/2-oxoglutarate-dependent enzyme from Arabidopsis thaliana.
abstract: The crystal structure of the gene product of At3g21360 from Arabidopsis thaliana was determined by the single-wavelength anomalous dispersion method and refined to an R factor of 19.3% (Rfree = 24.1%) at 2.4 A resolution. The crystal structure includes two monomers in the asymmetric unit that differ in the conformation of a flexible domain that spans residues 178-230. The crystal structure confirmed that At3g21360 encodes a protein belonging to the clavaminate synthase-like superfamily of iron(II) and 2-oxoglutarate-dependent enzymes. The metal-binding site was defined and is similar to the iron(II) binding sites found in other members of the superfamily.
authors.affiliation: Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin-Madison, USA.
authors.forename: Eduard
authors.initials: E
authors.lastname: Bitto
authors.forename: Craig A
authors.initials: CA
authors.lastname: Bingman
authors.forename: Simon T M
authors.initials: ST
authors.lastname: Allard
authors.forename: Gary E
authors.initials: GE
authors.lastname: Wesenberg
authors.forename: David J
authors.initials: DJ
authors.lastname: Aceti
authors.forename: Russell L
authors.initials: RL
authors.lastname: Wrobel
authors.forename: Ronnie O
authors.initials: RO
authors.lastname: Frederick
authors.forename: Hassan
authors.initials: H
authors.lastname: Sreenath
authors.forename: Frank C
authors.initials: FC
authors.lastname: Vojtik
authors.forename: Won Bae
authors.initials: WB
authors.lastname: Jeon
authors.forename: Craig S
authors.initials: CS
authors.lastname: Newman
authors.forename: John
authors.initials: J
authors.lastname: Primm
authors.forename: Michael R
authors.initials: MR
authors.lastname: Sussman
authors.forename: Brian G
authors.initials: BG
authors.lastname: Fox
authors.forename: John L
authors.initials: JL
authors.lastname: Markley
authors.forename: George N
authors.initials: GN
authors.lastname: Phillips
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D003460:Crystallization; D018360:Crystallography, X-Ray; D054637:Dual-Specificity Phosphatases; D007501:Iron / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D007656:Ketoglutaric Acids; D007501:Iron; D006899:Mixed Function Oxygenases; C066046:clavaminate synthase; C481271:DsPTP1 protein, Arabidopsis; D054637:Dual-Specificity Phosphatases; D017027:Protein Tyrosine Phosphatases