title: Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection.
abstract: In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion of LSA-3 gene was cloned, and its recombinant protein (rLSA-3) was expressed in Escherichia coli and purified by column chromatography. The purified rLSA-3 and 120 test blood/serum samples collected from inhabitants in malaria-endemic areas of Mandalay, Myanmar were used for this study. In microscopic examinations of blood samples, P. falciparum positive rate was 39.1% (47/120) in thin smear trials, and 33.3% (40/120) in thick smear trials. Although the positive rate associated with the rLSA-3 (30.8%) was lower than that of the blood stage antigens (70.8%), rLSA-3 based enzyme-linked immunosorbent assay could detect 12 seropositive cases (10.0%), in which blood stage antigens were not detected. These results indicate that the LSA-3 is a useful antigen for an early serodiagnosis of P. falciparum infection.
authors.affiliation: Division of Tropical and Endemic Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul.
authors.forename: Hyeong-Woo
authors.initials: HW
authors.lastname: Lee
authors.forename: Sung-Ung
authors.initials: SU
authors.lastname: Moon
authors.forename: Hye-Sun
authors.initials: HS
authors.lastname: Ryu
authors.forename: Yeon-Joo
authors.initials: YJ
authors.lastname: Kim
authors.forename: Shin-Hyeong
authors.initials: SH
authors.lastname: Cho
authors.forename: Gyung-Tae
authors.initials: GT
authors.lastname: Chung
authors.forename: Khin
authors.initials: K
authors.lastname: Lin
authors.forename: Byoung-Kuk
authors.initials: BK
authors.lastname: Na
authors.forename: Yoon
authors.initials: Y
authors.lastname: Kong
authors.forename: Kyung-Suk
authors.initials: KS
authors.lastname: Chung
authors.forename: Tong-Soo
authors.initials: TS
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D017125:Genes, Protozoan / Q000235:genetics / Q000276:immunology; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood / Q000175:diagnosis*; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000276:immunology*; D010966:Plasmodium vivax / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D017931:DNA Primers; D016054:DNA, Protozoan; C063362:QF116 antigen, Plasmodium falciparum; D011994:Recombinant Proteins; C067302:liver stage-specific antigen, Plasmodium