title: Iron release and ROS generation from mineral particles are not related to cytokine release or apoptosis in exposed A549 cells.
abstract: The generation of reactive oxygen species (ROS) by mineral particles is believed to be central to their toxicity and their ability to induce inflammation. Surface bound or soluble iron may contribute to the particle-effects by enhancing the ROS generation through the Fenton reaction. Nevertheless, the importance of ROS and transition metals to mineral particle-induced effects is still unclear and further investigations are needed. In the present study we have investigated different mineral particles for their total iron content, amount of soluble iron at pH 7.0 and 4.0, their ability to generate ROS in a cell-free environment, and their ability to induce cytokine release and apoptosis in a human alveolar epithelial cell line (A549). All the investigated parameters varied considerably between the different particles, with the exception of ability to induce apoptosis. Total iron content did not reflect the amount of soluble iron, and neither total nor soluble iron was correlated with ROS generation. Moreover, iron content and ROS was not correlated with the ability of particles to induce cytokine release or apoptosis. The present results suggest that there is no clear relationship between the particles iron content and ability to generate ROS. Moreover, neither iron content nor the ability to induce ROS generation appears to be a prerequisite for the inflammatory potential or cytotoxicity of mineral particles.
authors.affiliation: Department of Air Pollution and Noise, Division of Environmental Medicine, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen, N-0403 Oslo, Norway. johan.ovrevik@fhi.no
authors.forename: J
authors.initials: J
authors.lastname: Ovrevik
authors.forename: R B
authors.initials: RB
authors.lastname: Hetland
authors.forename: R P
authors.initials: RP
authors.lastname: Schins
authors.forename: T
authors.initials: T
authors.lastname: Myran
authors.forename: P E
authors.initials: PE
authors.lastname: Schwarze
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008903:Minerals / Q000633:toxicity*; D010316:Particle Size; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000393:Air Pollutants; D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8; D008903:Minerals; D017382:Reactive Oxygen Species; D007501:Iron