Solution-stirring method improves crystal quality of human triosephosphate isomerase.
We examined the effect of a solution-stirring method on human triosephosphate isomerase crystallization. The crystals diffracted to more than 1.4 A resolution, whereas those obtained by the normal vapour-diffusion technique diffracted to 2.8 A. These results clearly show that the solution-stirring method is valuable and useful for protein crystallization because of its effectiveness and simplicity.
Adachi
Hiroaki
H
SOSHO Project (Crystal Design Project), Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Niino
Ai
A
Kinoshita
Takayoshi
T
Warizaya
Masaichi
M
Maruki
Riyo
R
Takano
Kazufumi
K
Matsumura
Hiroyoshi
H
Inoue
Tsuyoshi
T
Murakami
Satoshi
S
Mori
Yusuke
Y
Sasaki
Takatomo
T
D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray; D006801:Humans; D012996:Solutions / Q000737:chemistry; D014305:Triose-Phosphate Isomerase / Q000737:chemistry*; D014961:X-Ray Diffraction
D012996:Solutions; D014305:Triose-Phosphate Isomerase