Cytokinetics of secondary neurulation in chick embryos: Hamburger and Hamilton stages 16-45.
OBJECTIVE
In an attempt to understand the events in the secondary neurulation in embryonic stage, we investigated cytokinetic changes in the tail bud of normal developing chick embryos.


METHODS
We investigated chick embryos of Hamburger and Hamilton stages 16-45 by staining for proliferating cell nuclear antigen (PCNA) and applying the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) method.


CONCLUSIONS
The cytokinetics of secondary neurulation have several distinctive properties: a general chronological delay compared to primary neurulation; absence of ventrodorsal differences; larger rostrocaudal differences; and degeneration of the distal end of the spinal cord in the later stages of development. These differences in morphogenetic and cytokinetic behavior between primary and secondary neurulation may help to improve our understanding of anomalies originating during secondary neurulation.
Yang
Hee-Jin
HJ
Department of Neurosurgery, Seoul National University Boramae Hospital, Seoul, South Korea.
Wang
Kyu-Chang
KC
Chi
Je G
JG
Lee
Myung-Sook
MS
Lee
Yun-Jin
YJ
Kim
Seung-Ki
SK
Lee
Chang Sub
CS
Cho
Byung-Kyu
BK
D000367:Age Factors; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002454:Cell Differentiation; D002490:Central Nervous System / Q000166:cytology* / Q000196:embryology*; D002642:Chick Embryo; D048749:Cytokinesis / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D020287:In Situ Nick-End Labeling / Q000379:methods; D009474:Neurons / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
D018809:Proliferating Cell Nuclear Antigen