A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA).
MicroRNAs represent a class of short (approximately 22 nt), noncoding regulatory RNAs involved in development, differentiation, and metabolism. We describe a novel microarray platform for genome-wide profiling of mature miRNAs (miChip) using locked nucleic acid (LNA)-modified capture probes. The biophysical properties of LNA were exploited to design probe sets for uniform, high-affinity hybridizations yielding highly accurate signals able to discriminate between single nucleotide differences and, hence, between closely related miRNA family members. The superior detection sensitivity eliminates the need for RNA size selection and/or amplification. MiChip will greatly simplify miRNA expression profiling of biological and clinical samples.
Castoldi
Mirco
M
Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany.
Schmidt
Sabine
S
Benes
Vladimir
V
Noerholm
Mikkel
M
Kulozik
Andreas E
AE
Hentze
Matthias W
MW
Muckenthaler
Martina U
MU
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D015152:Blotting, Northern; D015342:DNA Probes; D020869:Gene Expression Profiling / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D012680:Sensitivity and Specificity
D015342:DNA Probes; D035683:MicroRNAs; D009696:Nucleic Acids; D015345:Oligonucleotide Probes