Identification of differently expressed genes in human colorectal adenocarcinoma.
OBJECTIVE
To investigate the differently expressed genes in human colorectal adenocarcinoma.


METHODS
The integrated approach for gene expression profiling that couples suppression subtractive hybridization, high-throughput cDNA array, sequencing, bioinformatics analysis, and reverse transcriptase real-time quantitative polymerase chain reaction (PCR) was carried out. A set of cDNA clones including 1260 SSH inserts amplified by PCR was arrayed using robotic printing. The cDNA arrays were hybridized with fluorescent-labeled probes prepared from RNA of human colorectal adenocarcinoma (HCRAC) and normal colorectal tissues.


RESULTS
A total of 86 genes were identified, 16 unknown genes and 70 known genes. The transcription factor Somult9 influencing cell differentiation was downregulated. At the same time, Heat shock protein 10 KDis downregulated and Calmoulin is up-regulated.


CONCLUSIONS
Downregulation of heat shock protein 10 KD lost its inhibition of Ras, and then attenuated the Ras GTPase signaling pathway, increased cell proliferation and inhibited cell apoptosis. Down-regulated transcription factor Somult9 influences cell differentiation and cell-specific gene expression. Down-regulated Somult9 also decreases its binding to calmodulin, accumulates calmodulin as receptor-activated kinase and phosphorylase kinase due to the activation of PhK.
Chen
Yao
Y
Department of Anatomy, Basic and Legal Medical Institute, West China Medical Center, Sichuan University, Chengdu 610041, Sichuan Province, China. xmxfh@263.net
Zhang
Yi-Zeng
YZ
Zhou
Zong-Guang
ZG
Wang
Gang
G
Yi
Zeng-Ni
ZN
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D017209:Apoptosis / Q000235:genetics; D002147:Calmodulin / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D004273:DNA, Neoplasm / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm; D006609:High Mobility Group Proteins / Q000235:genetics / Q000502:physiology; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055758:SOX9 Transcription Factor; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D020703:ras GTPase-Activating Proteins / Q000502:physiology; D018631:ras Proteins / Q000235:genetics / Q000502:physiology
D002147:Calmodulin; D004273:DNA, Neoplasm; D006609:High Mobility Group Proteins; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D014157:Transcription Factors; D020703:ras GTPase-Activating Proteins; D018631:ras Proteins