Molecular identification of Candida dubliniensis isolated from oral lesions of HIV-positive and HIV-negative patients in São Paulo, Brazil.
Candida dubliniensis is a new, recently described species of yeast. This emerging oral pathogen shares many phenotypic and biochemical characteristics with C. albicans, making it hard to differentiate between them, although they are genotypically distinct. In this study, PCR (Polymerase Chain Reaction) was used to investigate the presence of C. dubliniensis in samples in a culture collection, which had been isolated from HIV-positive and HIV-negative patients with oral erythematous candidiasis. From a total of 37 samples previously identified as C. albicans by the classical method, two samples of C. dubliniensis (5.4%) were found through the use of PCR. This study underscores the presence of C. dubliniensis, whose geographical and epidemiological distribution should be more fully investigated.
Chavasco
Jorge Kleber
JK
Universidade Federal de Alfenas, UNINCOR, MG, Brazil. jkchavasco@uol.com.br
Paula
Claudete Rodrigues
CR
Hirata
Mario Hiroyuki
MH
Aleva
Natanael Atilas
NA
Melo
Carlos Eduardo de
CE
Gambale
Walderez
W
Ruiz
Luciana da Silva
Lda S
Franco
Marília Caixeta
MC
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000382:microbiology*; D001938:Brazil; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000175:diagnosis / Q000382:microbiology*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
D004271:DNA, Fungal