Inhibitory effects of Akacid (plus) on growth and aflatoxin production by Aspergillus parasiticus.
The effects of Akacid plus, a novel guanidine-based polymer first introduced as a biocidal and disinfectant agent were studied on Aspergillus parasiticus growth and its aflatoxin (AF) productivity. The fungus was cultured on yeast extract-sucrose (YES) broth in presence of various twofold serial dilutions of 25% Akacid plus (1.5-96 microL/50 mL medium) and then incubated in shaking condition with 150 rev./min at 28 degrees C for 96 h. Based on obtained results, Akacid plus was found to significantly inhibit both growth and aflatoxin B1 (AFB1) synthesis in very low concentrations in a dose-dependent manner. Fungal growth inhibition was determined in the range of 9.6-99.6% in mycelia exposed to the total concentration range of 1.5-48 microL. A final concentration of 96 microL was necessary to completely inhibit the growth of fungus. Under similar conditions, AFB1 synthesis was found to be strongly inhibited by 8.1-98.0% in presence of 1.5-24 microL Akacid plus with a maximum of 100% by 48 microL concentration. With respect to the unique physico-chemical properties of Akacid plus, its marked inhibitory effects on A. parasiticus growth and its AFB1 synthesis shown for the first time in this study make it a promising candidate for application in prevention programmes of AF contamination of susceptible crops.
Razzaghi-Abyaneh
Mehdi
M
Department of Mycology, Pasteur Institute of Iran, Tehran, 13164, Iran. mrab442@yahoo.com
Shams-Ghahfarokhi
Masoomeh
M
Eslamifar
Ali
A
Schmidt
Oskar J
OJ
Gharebaghi
Reza
R
Karimian
Mehdi
M
Naseri
Alireza
A
Sheikhi
Mohammad
M
D016604:Aflatoxin B1 / Q000378:metabolism*; D000935:Antifungal Agents / Q000494:pharmacology; D001230:Aspergillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*
C512857:AKACID Plus; D000935:Antifungal Agents; D006146:Guanidines; D011108:Polymers; D016604:Aflatoxin B1