Encircled: large-scale purification of replication origins from Mammalian chromosomes.
A novel cloning strategy for sequences comprising mammalian replication origins, described by Mesner et al. (2006) in a recent issue of Molecular Cell, utilizes an origin trapping assay in which replication bubbles are selectively retained in agarose due to their circular nature.
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, 55455, USA.
Anja-Katrin
AK
Bielinsky
Miruthubashini
M
Raveendranathan
D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D005057:Eukaryotic Cells; D015723:Gene Library; D018741:Replication Origin / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D013762:Tetrahydrofolate Dehydrogenase