High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats.
Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.
Commonwealth Scientific and Industrial Research Organization, Food Futures National Research Flagship, P.O. Box 93, North Ryde 1670, NSW, Australia.
Ahmed
A
Regina
Anthony
A
Bird
David
D
Topping
Sarah
S
Bowden
Judy
J
Freeman
Tina
T
Barsby
Behjat
B
Kosar-Hashemi
Zhongyi
Z
Li
Sadequr
S
Rahman
Matthew
M
Morell
D015061:1,4-alpha-Glucan Branching Enzyme / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000688:Amylose / Q000378:metabolism*; D000818:Animals; D004032:Diet; D030841:Food, Genetically Modified; D005818:Genetic Engineering; D005936:Glucans / Q000737:chemistry; D006304:Health Status; D006801:Humans; D007420:Intestine, Large / Q000378:metabolism; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008297:Male; D030821:Plants, Genetically Modified; D034622:RNA Interference; D051381:Rats; D017207:Rats, Sprague-Dawley; D013213:Starch / Q000737:chemistry / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000378:metabolism*
D005936:Glucans; D007527:Isoenzymes; D013213:Starch; D000688:Amylose; D015061:1,4-alpha-Glucan Branching Enzyme