Mutation of phosphotransacetylase but not isocitrate lyase reduces the virulence of Salmonella enterica serovar Typhimurium in mice.
A phosphotransacetylase (pta) mutant of Salmonella enterica serovar Typhimurium was attenuated in mice but survived normally in macrophages. Complementation of the pta mutation in trans restored virulence. An isocitrate lyase (aceA) mutant was virulent, so the inability to use acetate as a sole carbon source does not explain the phenotype.
VA Healthcare San Diego, 3350 La Jolla Village Dr., San Diego, CA 92161, USA.
Yang Re
YR
Kim
Shaun R
SR
Brinsmade
Zheng
Z
Yang
Jorge
J
Escalante-Semerena
Joshua
J
Fierer
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D007522:Isocitrate Lyase / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D009154:Mutation; D010709:Phosphate Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D012480:Salmonella Infections / Q000382:microbiology / Q000401:mortality; D012486:Salmonella typhimurium / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence
D001426:Bacterial Proteins; D010709:Phosphate Acetyltransferase; D007522:Isocitrate Lyase