Refractive index matching applied to fecal smear clearing.
Thick smears of human feces can be made adequate for identification of helminth eggs by means of refractive index matching. Although this effect can be obtained by simply spreading a fleck of feces on a microscope slide, a glycerol solution has been routinely used to this end. Aiming at practicability, a new quantitative technique has been developed. To enhance both sharpness and contrast of the images, a sucrose solution (refractive index = 1.49) is used, which reduces the effect of light-scattering particulates. To each slide a template-measured (38.5 mm3) fecal sample is transferred. Thus, egg counts and sensitivity evaluations are easily made.
Instituto de Medicina Tropical de São Paulo, SP, Brasil.
Claúdio S
CS
Ferreira
D000818:Animals; D005243:Feces / Q000469:parasitology*; D006376:Helminths / Q000302:isolation & purification*; D006801:Humans; D010270:Parasite Egg Count / Q000379:methods; D012031:Refractometry; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods*; D013395:Sucrose
D013395:Sucrose