Cryopreserved tracheal grafts: a review of the literature.
Cryopreserved tracheal grafts have been used in several experimental models of long segment replacement. The clinical application of the procedure has been limited due to the fact that contradictory results have been reported. The purpose of this article is to present a review of the literature on tracheal cryopreservation. Despite the fact that most authors indicate that cryopreserved tracheal allografts retain viability and have a low immunological response, though they continue to function after transplantation with good epithelialization and patency, cryopreservation leads to significant damage to cartilage, the degree of which is based on the freezing-storage methods that affect the function and durability of a graft. The long-term storage of cartilage must therefore be investigated in more detail in basic research models of cartilage viability: the evaluation of chondrocyte apoptosis, and the use of different solutions for tracheal cryopreservation other than RPMI-1640, Dulbecco's modified Eagle's, Eurocollins, and TC-199. Furthermore, problems that involve improving the blood supply to the graft after extensive resection and immunosuppression must be resolved before tracheal cryopreservation can become a clinically established method for tracheal grafts.
Department of Experimental Surgery Research, National Institute of Respiratory Diseases, Doctoral Program in Biological Sciences, Autonomous Metropolitan University-Xochimilco, Mexico.
Avelina
A
Sotres-Vega
Jaime
J
Villalba-Caloca
Rogelio
R
Jasso-Victoria
J Raúl
JR
Olmos-Zúñiga
Miguel
M
Gaxiola-Gaxiola
Matilde
M
Baltazares-Lipp
Alfredo
A
Santibañez-Salgado
Patricio
P
Santillán-Doherty
D000818:Animals; D015925:Cryopreservation; D003451:Cryoprotective Agents; D006085:Graft Survival; D013696:Temperature; D013997:Time Factors; D014132:Trachea / Q000098:blood supply / Q000473:pathology / Q000637:transplantation*; D014184:Transplantation, Homologous / Q000276:immunology; D019737:Transplants
D003451:Cryoprotective Agents