Isolation of a novel strain of Butyrivibrio fibrisolvens that isomerizes linoleic acid to conjugated linoleic acid without hydrogenation, and its utilization as a probiotic for animals.
OBJECTIVE
Isolation of a new strain of Butyrivibrio fibrisolvens possessing great capacity to produce conjugated linoleic acid (CLA) in order to utilize as a probiotic for animals.


RESULTS
A novel strain (MDT-5) was isolated from the goat rumen, which exclusively converted linoleic acid (LA) to CLA, because of its high LA isomerase activity with virtually no CLA reductase activity. MDT-5 also converted linolenic acid to conjugated linolenic acid that may be more bioactive than CLA. The oral administration of MDT-5 every other day to mice for 2 weeks resulted in increased amounts of CLA in the contents of the large intestine (2.5-fold), as well as in adipose tissue (threefold). Feeding a high-LA diet, as well as prolonging the period of MDT-5 administration, further increased the CLA content in body fat.


CONCLUSIONS
MDT-5 has by far greater ability to produce CLA than any other known bacteria. Administration of MDT-5 to mice increases CLA production in the large intestine, which results in increased CLA absorption.


CONCLUSIONS
MDT-5 may be useful in pet animals as a probiotic to provide CLA continuously.
Department of Life Science, Meiji University, Kawasaki, Japan.
S
S
Fukuda
Y
Y
Suzuki
M
M
Murai
N
N
Asanuma
T
T
Hino
D000273:Adipose Tissue / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D045856:Butyrivibrio / Q000302:isolation & purification*; D004032:Diet; D006041:Goats; D007420:Intestine, Large / Q000378:metabolism; D007536:Isomerism; D019787:Linoleic Acid / Q000008:administration & dosage / Q000378:metabolism; D008041:Linoleic Acids / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000032:analysis / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D019936:Probiotics / Q000008:administration & dosage*; D012417:Rumen / Q000382:microbiology
D008041:Linoleic Acids; D044243:Linoleic Acids, Conjugated; D019787:Linoleic Acid