Expression of nuclear factor-kappaB in traumatic cataract.
OBJECTIVE
To study the differences in expression of nuclear factor-kappaB (NF-kappaB) between human traumatic cataract and normal lenticular epithelial cells.


METHODS
Total RNA of anterior capsule specimens was taken under the microscope from normal cadaveric eyes donors and those suffering from traumatic cataract to make semi-quantitative RT-PCR and conduct analysis of differences in expression of NF-kappaB between them.


RESULTS
As compared with the mean of 0.8337 in normal control group, the expression equivalent of NF-kappaB was 0.9074 for the lenticular epithelial cells in traumatic cataract sufferers, and the differences are of noticeable significance (t = 2.447, P<0.05) accordingly.


CONCLUSIONS
NF-kappaB is likely a kind of transcription factor necessary to maintain metabolism of normal lenticular epithelial cells. Higher NF-kappaB available in the traumatic cataract sufferer's lenticular epithelial cells means NF-kappaB is of possible relevance to occurrence and development of traumatic cataract.
Department of Ophthalmology, Affiliated Hospital of Qingdao University Medical School, Qingdao 266000, China.
Qian
Q
Yang
Gui-Qiu
GQ
Zhao
Qian
Q
Li
Xiang-Ping
XP
Liu
D000293:Adolescent; D000328:Adult; D002386:Cataract / Q000235:genetics / Q000378:metabolism* / Q000628:therapy; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D007908:Lens, Crystalline / Q000293:injuries* / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction
D016328:NF-kappa B