Plant regeneration through somatic embryogenesis from tissues of mature oak trees: true-to-type conformity of plantlets by RAPD analysis.
Somatic embryogenesis was induced in expanding leaf explants excised from epicormic shoots forced from branch segments taken at four different times of year from a mature oak (Quercus robur L.). Branch segments 2-4 cm in diameter produced most shoots when collected in March. Somatic embryos were induced on explants derived from branches of all collection dates, although collection in November seemed to afford the best results. Germination and conversion ability of embryos of embryogenic lines derived from six oak trees depended heavily on genotype, conversion rates ranging from 0 to 70%. RAPD analyses found no evidence of genetic variation either within or between the embryogenic lines established from three of these trees, or between these lines and the trees of origin, or between somatic embryo derived plantlets and the trees of origin. The embryogenic system used in this study appears to be suitable for true-to-type clonal propagation of mature oak genotypes.
Valladares
S
S
Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain.
Sánchez
C
C
Martínez
M T
MT
Ballester
A
A
Vieitez
A M
AM
D003470:Culture Media; D018525:Germination; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development; D029963:Quercus / Q000187:drug effects / Q000196:embryology* / Q000502:physiology*; D019105:Random Amplified Polymorphic DNA Technique; D012038:Regeneration / Q000502:physiology*; D046509:Tissue Culture Techniques
D003470:Culture Media; D010937:Plant Growth Regulators