An optical biosensor for rapid and label-free detection of cells.
We report a broadly applicable optical method for rapid and label-free detection of as few as 45 cells. In this method, bacterial cells are detected by measuring the amount of laser light transmitted through a small glass well functionalized with antibodies which specifically recognize and capture the cells. The described approach is simple, rapid, economical, and promising for portable and high-throughput detection of a wide variety of pathogenic and infectious cells.
School of Mechanical Engineering, and Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana 47907, USA.
Ghanashyam
G
Acharya
Chun-Li
CL
Chang
Cagri
C
Savran
D000907:Antibodies, Bacterial / Q000737:chemistry; D000918:Antibody Specificity; D001431:Bacteriological Techniques / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*
D000907:Antibodies, Bacterial