Preparation and antimicrobial activity of gelatin microparticles containing propolis against oral pathogens.
Gelatin microparticles containing propolis ethanolic extractive solution were prepared by spray-drying technique. Particles with regular morphology, mean diameter ranging of 2.27 microm to 2.48 microm, and good entrapment efficiency for propolis were obtained. The in vitro antimicrobial activity of microparticles was evaluated against microorganisms of oral importance (Enterococcus faecalis, Streptococcus salivarius, Streptococcus sanguinis, Streptococcus mitis, Streptococcus mutans, Streptococcus sobrinus, Candida albicans, and Lactobacillus casei). The utilized techniques were diffusion in agar and determination of minimum inhibitory concentration. The choice of the method to evaluate the antimicrobial activity of microparticles showed be very important. The microparticles displayed activity against all tested strains of similar way to the propolis, showing greater activity against the strains of E. salivarius, S. sanguinis, S. mitis, and C. albicans.
Bruschi
M L
ML
Programa de Pós-Graduação em Ciências Farmacêuticas, FCFRP- USP, Av. Zeferino Vaz, s/n, Ribeirão Preto, SP, CEP 1404-0903, Brazil. bruschimi@yahoo.com.br
Lara
E H G
EH
Martins
C H G
CH
Vinholis
A H C
AH
Casemiro
L A
LA
Panzeri
H
H
Gremião
M P D
MP
D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000627:therapeutic use*; D002176:Candida albicans / Q000187:drug effects; D004337:Drug Carriers; D000431:Ethanol / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D007780:Lacticaseibacillus casei / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D011429:Propolis / Q000008:administration & dosage* / Q000627:therapeutic use*; D013289:Streptococcaceae / Q000187:drug effects
D000890:Anti-Infective Agents; D004337:Drug Carriers; D000431:Ethanol; D005780:Gelatin; D011429:Propolis