Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of gentically modified crops. 3. Assessing unintended effects.
The current procedures to assess the safety of food and feed derived from modern biotechnology include the investigation of possible unintended effects. To improve the probability of detecting unintended effects, profiling techniques such as proteomics are currently tested as complementary analytical tools to the existing safety assessment. An optimized two-dimensional gel electrophoresis (2DE) method was used as a proteomics approach to investigate insertional and pleiotropic effects on the proteome due to genetic engineering. Twelve transgenic Arabidopsis thaliana lines were analyzed by 2DE, and their seed proteomes were compared to that of their parental line as well as to 12 Arabidopsis ecotype lines. The genetic modification of the Arabidopsis lines, using three different genes and three different promoters, did not cause unintended changes to the analyzed seed proteome. Differences in spot quantity between transgenic and nontransgenic lines fell in the range of values found in the 12 Arabidopsis ecotype lines or were related to the introduced gene.
Product Safety Center, Monsanto Company, 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, USA. m.ruebelt@gmail.com
Martin C
MC
Ruebelt
Markus
M
Lipp
Tracey L
TL
Reynolds
Jon J
JJ
Schmuke
James D
JD
Astwood
Dean
D
DellaPenna
Karl-Heinz
KH
Engel
Klaus-Dieter
KD
Jany
D017360:Arabidopsis / Q000737:chemistry* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005818:Genetic Engineering / Q000009:adverse effects*; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*
D020543:Proteome