Induction of hypericins in Hypericum perforatum in response to chromium.
Seedlings of Hypericum perforatum were grown with 0.01 and 0.1 mM of chromium added to the nutrient media. A treatment with 0.01 mM Cr(VI) for seven days resulted in an increased production of protopseudohypericin (+135%), hypericin (+38%) and pseudohypericin (+5%). Treatment with 0.1 mM Cr(VI) for two days also caused an increase of protopseudohypericin (+167%), hypericin (25%) and pseudohypericin (+5%). The greatest effect of chromium treatment was observed at a concentration of 0.1 mM for seven days: protopseudohypericin increased +404% and pseudohypericin to +379%. Hypericin was not affected by this treatment.
Institute of Botany, University of Urbino, Via Bramante, 28, 61029 Urbino, Italy. tirillini@uniurb.it
Bruno
B
Tirillini
Antonio
A
Ricci
Giorgio
G
Pintore
Mario
M
Chessa
Simona
S
Sighinolfi
D000704:Analysis of Variance; D000873:Anthracenes; D002857:Chromium / Q000032:analysis / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D020902:Hypericum / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D010569:Perylene / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism*; D010946:Plants, Medicinal / Q000187:drug effects* / Q000378:metabolism; D036226:Seedlings / Q000737:chemistry / Q000187:drug effects
D000873:Anthracenes; D002857:Chromium; C108324:protopseudohypericin; D010569:Perylene; C004965:hypericin; C056602:pseudohypericin