Determination of paralytic shellfish toxins in dinoflagellate Alexandrium tamarense by using isotachophoresis/capillary electrophoresis.
Baseline separation of seven paralytic shellfish toxins (PSTs), namely decarbamoylsaxitoxin (dcSTX), saxitoxin (STX), neosaxitoxin (NEO), gonyautoxin-2 (GTX-2), gonyautoxin-3 (GTX-3), gonyautoxin-1 (GTX-1), and gonyautoxin-4 (GTX-4), was achieved by using capillary ITP (CITP)/CE with UV detection. Separation parameters including duration time and voltage in CITP process, separation voltage, and pH and concentration of buffer were optimized. The developed method provided linear responses from 1.3 to 200 microM for the PSTs. The LOD ranged from 0.1 to 0.3 microM. PST extracts from two algal strains of Alexandrium tamarense were analyzed and the toxin concentrations in the samples were quantified with an internal standard method by using NEO as the internal standard. The algal extract of A. tamarense HK9301 contained 332 microM GTX-2 and 224 microM GTX-3, while the PSTs were not detected in the extract of A. tamarense CI01.
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
Youyi
Y
Wu
Alvin Yam Tat
AY
Ho
Pei-Yuan
PY
Qian
Kelvin Sze-Yin
KS
Leung
Zongwei
Z
Cai
Jin-Ming
JM
Lin
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002021:Buffers; D002457:Cell Extracts; D004141:Dinoflagellida / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008387:Marine Toxins / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D010243:Paralysis / Q000139:chemically induced; D013997:Time Factors
D002021:Buffers; D002457:Cell Extracts; D008387:Marine Toxins