Microdeformational wound therapy: effects on angiogenesis and matrix metalloproteinases in chronic wounds of 3 debilitated patients.
The vacuum-assisted closure (VAC) device causes microdeformations of the wound surface in contact with the foam. Because angiogenesis and matrix metalloproteinase (MMP) activity are altered in chronic wounds, we hypothesized that microdeformations stimulate capillary formation and affect MMP activity. A VAC device was used to deliver microdeformational wound therapy (MDWT) to the chronic wounds of 3 debilitated patients. Debrided tissue was obtained from wound areas with and without foam contact. Microvessel density and MMP activity were determined by immunohistochemistry and zymography, respectively. Microvessel density of MDWT-treated wounds was 4.5% (+/-0.8) compared with areas not covered by foam [1.6% (+/-0.1)] (P = 0.05) during the first week of treatment and 2.7% (+/-0.3) compared with untreated tissue [1.3% (+/-0.1)] (P = 0.03) during the second treatment week. Wounds subjected to MDWT had greater microvessel density compared with the same wound prior to treatment [1.5% (+/-0.3)] (P = 0.02). MMP-9/NGAL (neutrophil gelatinase-associated lipocalin), MMP-9, latent MMP-2, and active MMP-2 were reduced by 15%-76% in MDWT-treated wounds. MDWT provides a favorable wound-healing environment by increasing angiogenesis and decreasing MMP activity in chronic wounds.
Division of Plastic Surgery, Harvard Plastic Surgery Program, Brigham and Women's Hospital, Boston, MA 02115, USA.
Arin K
AK
Greene
Mark
M
Puder
Roopali
R
Roy
Danielle
D
Arsenault
Stephanie
S
Kwei
Marsha A
MA
Moses
Dennis P
DP
Orgill
D000368:Aged; D001706:Biopsy; D002196:Capillaries / Q000166:cytology; D002452:Cell Count; D002908:Chronic Disease; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000502:physiology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology; D019651:Plastic Surgery Procedures / Q000295:instrumentation*; D014945:Wound Healing / Q000502:physiology*; D014947:Wounds and Injuries / Q000276:immunology / Q000473:pathology* / Q000628:therapy*
D019408:Platelet Endothelial Cell Adhesion Molecule-1; D020782:Matrix Metalloproteinases