Determination and characterization of bovine interleukin-17 cDNA.
Interleukin-17 (IL-17) is a proinflammatory cytokine produced by activated memory T cells, and it appears to play an upstream role in T cell-triggered inflammation by stimulating stromal cells to secrete other cytokines. We hypothesize that IL-17 plays a role in the recruitment of neutrophils in the bovine mammary gland during infection or immune-mediated inflammation. The rapid amplification of cDNA ends (RACE) method was used to obtain a cDNA of bovine IL-17 (BoIL-17) containing a 462-bp open reading frame (ORF) encoding a protein of 153 amino acids (aa) with a molecular mass of 17.2 kDa, a 23-residue NH(2)-terminal signal peptide, a single potential N-linked glycosylation site, and 6 cysteine residues. BoIL-17 protein shared 73.5% identity with the human protein and 67% with the mouse and rat proteins. Sf9 insect cells were transfected with BoIL-17 cDNA, and supernatant was tested for biologic activity on a primary culture of bovine mammary epithelial cells (MECs). mRNA synthesis of IL-6, IL-8, and growth-related oncogene alpha (Groalpha) was induced, suggesting a functional role for IL-17 in mammary immunity.
Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France. riollet@tours.inra.fr
Céline
C
Riollet
Doriane
D
Mutuel
Martine
M
Duonor-Cérutti
Pascal
P
Rainard
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D020029:Base Pairing; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D019743:Chemokines, CXC / Q000096:biosynthesis; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003545:Cysteine / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics*; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D020381:Interleukin-17 / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D008321:Mammary Glands, Animal / Q000166:cytology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D021382:Protein Sorting Signals; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera / Q000166:cytology / Q000821:virology
D019743:Chemokines, CXC; D018076:DNA, Complementary; D020381:Interleukin-17; D015850:Interleukin-6; D016209:Interleukin-8; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011994:Recombinant Proteins; D003545:Cysteine