[Effect of guizhi decoction on PKA and PKC activities of hypothalamus in fever rats].
OBJECTIVE
To investigate the changes of the activity of both protein kinase A and C and the mechanisms of antipyretic action of Guizhi decoction.


METHODS
The fever responses were observed after combination injection of H-89 (a selective inhibitor of PKA) and calphostin C (a selective inhibitor of PKC), and oral pretreatment of Guizhi decoction in fever rats induced by an intra-cerebroventricular (icv) injection of an EP3 agonist, and both PKA and PKC activity in hypothalamus were measured in rats pretreated with Guizhi decoction and vehicle using isotopic tracing assay.


RESULTS
The rise in rat body temperature was inhibited by H-89, Calphostin C, and Guizhi decoction, moreover, pretreatment with Guizhi decoction reduced PKA activity obviously. PKC activity in model rats exhibited a tendency to drop compared with that of control group, Oral administration of Guizhi decoction in large dose inhibited the response significantly, while the low dose of Guzhi decoction has no effect on PKC.


CONCLUSIONS
Both PKA and PKC may participate in the mechanism of fever induction by EP3 agonist. The decrease of PKA and PKC may contribute to the antipyretic action of Guizhi decoction, some isoenzyme of PKC may play a role in the fever production.
Zhou
Jun
J
Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing.
Li
Cang-hai
CH
Huo
Hai-ru
HR
Kang
Xu-liang
XL
Li
Lan-fang
LF
Jiang
Nan
N
Jiang
Ting-lian
TL
D018712:Analgesics, Non-Narcotic / Q000494:pharmacology; D000818:Animals; D032904:Cinnamomum aromaticum / Q000737:chemistry; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D015232:Dinoprostone / Q000031:analogs & derivatives; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005334:Fever / Q000139:chemically induced / Q000201:enzymology*; D007031:Hypothalamus / Q000201:enzymology*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D011493:Protein Kinase C / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018078:Receptors, Prostaglandin E / Q000819:agonists; D058308:Receptors, Prostaglandin E, EP3 Subtype
D018712:Analgesics, Non-Narcotic; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C545649:Ptger3 protein, rat; D018078:Receptors, Prostaglandin E; D058308:Receptors, Prostaglandin E, EP3 Subtype; C016767:sulprostone; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D015232:Dinoprostone