Transcript profiles of Nitrosomonas europaea during growth and upon deprivation of ammonia and carbonate.
The transcriptome of Nitrosomonas europaea was analyzed with whole-genome microarrays. Growing cells were compared to cells deprived of (NH4)2SO4 and Na2CO3. Hybridization signals were detected for 76% of the genes represented on the array under either or both conditions. Transcript levels for 68% of the genes were at least twofold higher in growing cells than in deprived cells, while only 0.42% of the genes were present at more than twofold higher levels in deprived cells. Transcript levels for the remaining 7% of the genes did not change significantly with the treatments. These trends were confirmed for selected genes by Northern hybridizations and quantitative RT-PCR. Compared to heterotrophic bacteria, N. europaea downregulates a greater proportion of its genes and fewer genes appear to be associated with the adaptation to starvation.
Wei
Xueming
X
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA.
Yan
Tingfen
T
Hommes
Norman G
NG
Liu
Xiuduan
X
Wu
Liyou
L
McAlvin
Crystal
C
Klotz
Martin G
MG
Sayavedra-Soto
Luis A
LA
Zhou
Jizhong
J
Arp
Daniel J
DJ
D000641:Ammonia / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002254:Carbonates / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D018869:Heat-Shock Response; D043370:Nitrosomonas europaea / Q000254:growth & development* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002254:Carbonates; D000641:Ammonia