Molecular characterization of beta-lactamase genes blaA and blaB of Yersinia enterocolitica biovar 1A.
The beta-lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction-restriction fragment length polymorphism revealed genetic heterogeneity in blaA but not in blaB. Cluster analysis of blaA restriction profiles grouped the strains into three groups. The blaA gene of Y. enterocolitica biovar 1A showed a high degree of sequence homology to that of Y. enterocolitica 8081 (biovar 1B) and Y. enterocolitica Y-56 (biovar 4), whereas homology was low with class A beta-lactamase genes of other members of the family Enterobacteriaceae. The pI 8.7 of enzyme Bla-A of Y. enterocolitica biovar 1A was similar to that of biovars 2, 3 and 4. The enzyme Bla-B focused at 6.8 and 7.1, indicating that biovar 1A strains produced a 'B-like' enzyme. This is the first study to have investigated the genetic heterogeneity of the beta-lactamase genes of Y. enterocolitica.
Microbial Pathogenicity Laboratory, Department of Microbiology, University of Delhi South Campus, New Delhi, India.
Sachin
S
Sharma
Shilpi
S
Mittal
Sarita
S
Mallik
Jugsharan S
JS
Virdi
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D016000:Cluster Analysis; D004783:Environmental Microbiology; D005060:Europe; D006801:Humans; D007194:India; D008460:Meat / Q000382:microbiology; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D017385:Sequence Homology; D013552:Swine / Q000382:microbiology; D014481:United States; D015009:Yersinia Infections / Q000382:microbiology; D015008:Yersinia enterocolitica / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; D001618:beta-Lactamases