The tick plasma lectin, Dorin M, is a fibrinogen-related molecule.
A lectin, named Dorin M, previously isolated and characterized from the hemolymph plasma of the soft tick, Ornithodoros moubata, was cloned and sequenced. The immunofluorescence using confocal microscopy revealed that Dorin M is produced in the tick hemocytes. A tryptic cleavage of Dorin M was performed and the resulting peptide fragments were sequenced by Edman degradation and/or mass spectrometry. Two of three internal peptide sequences displayed a significant similarity to the family of fibrinogen-related molecules. Degenerate primers were designed and used for PCR with hemocyte cDNA as a template. The sequence of the whole Dorin M cDNA was completed by the method of RACE. The tissue-specific expression investigated by RT-PCR revealed that Dorin M, in addition to hemocytes, is significantly expressed in salivary glands. The derived amino-acid sequence clearly shows that Dorin M has a fibrinogen-like domain, and exhibited the most significant similarity with tachylectins 5A and 5B from a horseshoe crab, Tachypleus tridentatus. In addition, other protein and binding characteristics suggest that Dorin M is closely related to tachylectins-5. Since these lectins have been reported to function as non-self recognizing molecules, we believe that Dorin M may play a similar role in an innate immunity of the tick and, possibly, also in pathogen transmission by this vector.
Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological Sciences, University of South Bohemia, Branisovská 31, Ceské Budĕjovice 370 05, Czech Republic.
Ryan O M
RO
Rego
Vojtĕch
V
Kovár
Petr
P
Kopácek
Christoph
C
Weise
Petr
P
Man
Ivo
I
Sauman
Libor
L
Grubhoffer
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006434:Hemocytes / Q000737:chemistry; D019476:Insect Proteins / Q000032:analysis / Q000737:chemistry* / Q000502:physiology; D037102:Lectins / Q000097:blood* / Q000737:chemistry* / Q000502:physiology; D008969:Molecular Sequence Data; D026861:Ornithodoros / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012469:Salivary Glands / Q000378:metabolism; D016415:Sequence Alignment
D019476:Insect Proteins; D037102:Lectins