[Influence of different F1-specific components of Yersinia pestis capsular antigen on cell-mediated immunity].
The F1-specific components of Y. pestis capsular antigen, isolated by Baker's method, were shown to differ by their biological activity and the character of action on cell-mediated immunity factors, used in this study. Depending on the method of isolation, antigens could vary in the proportion of their components, which determined the specific features of the total preparations obtained in this investigation. Out of the four components under study having the same antigenic specificity, but different physico-chemical characteristics and genetically determined synthesis, only one component exhibited biological properties dominating in the secreted form of F1, more similar in their composition to protein Caf1. The other three components exhibited immunological activity on the level of unspecific protection in different ways, depending on the model, and influencing the outcome of the interaction "bacteria-macrophage". Lectin-like hemagglutinating and hemolyzing activity was shown by components not related to protein Caf1. The multi-component character of preparations F1 (Baker) and the individual activity of their components should be taken into consideration when using capsular antigen and different methods of its isolation for different aims.
L V
LV
Kosse
S A
SA
Lebedeva
A S
AS
Cherniavskaia
N A
NA
Shikulia
N S
NS
Eremenko
O K
OK
Bichul'
A N
AN
Terent'ev
D000818:Animals; D016667:Bacterial Capsules / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D006168:Guinea Pigs; D007111:Immunity, Cellular; D007279:Injections, Subcutaneous; D008213:Lymphocyte Activation; D017737:Macrophages, Peritoneal / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D010587:Phagocytosis; D010930:Plague / Q000276:immunology* / Q000517:prevention & control; D010931:Plague Vaccine / Q000008:administration & dosage / Q000276:immunology*; D011817:Rabbits; D014611:Vaccination; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology
D001426:Bacterial Proteins; D010931:Plague Vaccine; D022223:Vaccines, Subunit; C086725:caf1 protein, Yersinia pestis