A proteome reference map and proteomic analysis of Bifidobacterium longum NCC2705.
A comprehensive proteomic study was carried out to identify and characterize proteins expressed by Bifidobacterium longum NCC2705. A total of 708 spots representing 369 protein entries were identified by MALDI-TOF-MS and/or ESI-MS/MS. Isoelectric point values estimated by gel electrophoresis matched closely with their predicted ones, although some discrepancies exist suggesting that post-translational protein modifications might be common in B. longum. The identified proteins represent 21.4% of the predicted 1727 ORFs in the genome and correspond to 30% of the predicted proteome. Moreover 95 hypothetical proteins were experimentally identified. This is the first compilation of a proteomic reference map for the important probiotic organism B. longum NCC2705. The study aimed to define a number of cellular pathways related to important physiological processes at the proteomic level. Proteomic comparison of glucose- and fructose-grown cells revealed that fructose and glucose are catabolized via the same degradation pathway. Interestingly the sugar-binding protein specific to fructose (BL0033) and Frk showed higher levels of expression in cells grown on fructose than on glucose as determined by semiquantitative RT-PCR. BL0033 time course and concentration experiments showed that the induction time and fructose concentration correlates to increased expression of BL0033. At the same time, an ABC (ATP-binding cassette) transporter ATP-binding protein (BL0034) was slightly up-regulated in cells grown on fructose compared with glucose. All of the above results suggest that the uptake of fructose into the cell may be conducted by a specific transport system in which BL0033 might play an important role.
Yuan
Jing
J
Beijing Institute of Biotechnology, State Key Laboratory of Pathogen and Biosecurity, 100071 Beijing, China.
Zhu
Li
L
Liu
Xiankai
X
Li
Ting
T
Zhang
Ying
Y
Ying
Tianyi
T
Wang
Bin
B
Wang
Junjun
J
Dong
Hua
H
Feng
Erling
E
Li
Qiang
Q
Wang
Jie
J
Wang
Hongxia
H
Wei
Kaihua
K
Zhang
Xuemin
X
Huang
Cuifeng
C
Huang
Peitang
P
Huang
Liuyu
L
Zeng
Ming
M
Wang
Hengliang
H
D018528:ATP-Binding Cassette Transporters; D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis*; D001644:Bifidobacterium / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D006360:Heat-Shock Proteins / Q000032:analysis; D009711:Nucleotides / Q000378:metabolism; D016366:Open Reading Frames; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D018528:ATP-Binding Cassette Transporters; D000596:Amino Acids; D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D009711:Nucleotides; D020543:Proteome; D005632:Fructose; D005947:Glucose