RrgA and RrgB are components of a multisubunit pilus encoded by the Streptococcus pneumoniae rlrA pathogenicity islet.
The rlrA pathogenicity islet in Streptococcus pneumoniae TIGR4 encodes three surface proteins, RrgA, RrgB, and RrgC, and three sortase enzymes. Using transmission electron microscopy, cell fractionation, cell wall sorting signal domain swapping, and Western blotting, we show that RrgA and RrgB are incorporated into a multisubunit pilus in S. pneumoniae.
Howard Hughes Medical Institute and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.
Julianna
J
LeMieux
David L
DL
Hava
Alan
A
Basset
Andrew
A
Camilli
D019881:Aminoacyltransferases / Q000235:genetics / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000502:physiology; D036781:Fimbriae Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D010861:Fimbriae, Bacterial / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D044404:Genomic Islands / Q000235:genetics* / Q000276:immunology; D046529:Microscopy, Electron, Transmission; D017434:Protein Structure, Tertiary / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D037521:Virulence Factors / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
D001426:Bacterial Proteins; C579340:RrgA protein, Streptococcus pneumoniae; D037521:Virulence Factors; D036781:Fimbriae Proteins; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases