Probability of lethal damages of cryopreserved biological objects during storage.
This study demonstrated that with a cyclic change of the temperature from -196 degree C up to -130 degree C or -100 degree C part of cryopreserved microorganisms, tumor and human embryo cells died. This should be taken into account when moving and transporting the cryopreserved objects. Storage of samples under the above-mentioned stable temperatures did not result in additional death of cells.
Vysekantsev
I P
IP
Institute for problems of cryobiology and cryomedicine of the National Academy of Sciences of the Ukraine, 23 Pereyaslavskaya str., 61015, Kharkov, Ukraine. cryo@online.kharkov.ua
Gurina
T M
TM
Martsenyuk
V F
VF
Petrenko
T F
TF
Kudokotseva
E V
EV
Koshchiy
S V
SV
Groshevoy
M I
MI
D000818:Animals; D016923:Cell Death; D002470:Cell Survival; D015925:Cryopreservation; D006801:Humans; D008099:Liver / Q000166:cytology / Q000196:embryology; D008114:Liver Neoplasms, Experimental; D009420:Nervous System / Q000166:cytology / Q000196:embryology; D012441:Saccharomyces cerevisiae; D013211:Staphylococcus aureus; D014407:Tumor Cells, Cultured