A low-cost medium for mannitol production by Lactobacillus intermedius NRRL B-3693.
The production of mannitol by Lactobacillus intermedius NNRL B-3693 using molasses as an inexpensive carbon source was evaluated. The bacterium produced mannitol (104 g/l) from molasses and fructose syrups (1:1; total sugars, 150 g/l; fructose:glucose 4:1) in 16 h. Several kinds of inexpensive organic and inorganic nitrogen sources and corn steep liquor were evaluated for their potential to replace more expensive nitrogen sources derived from Bacto-peptone and yeast extract. Soy peptone D (5 g/l) and corn steep liquor (50 g/l) were found to be suitable substitutes for Bacto-peptone (5 g/l) and Bacto-yeast extract (5 g/l), respectively. The bacterium produced 105 g mannitol per liter from the molasses and fructose syrup (1:1, total sugars 150 g/l; fructose:glucose 4:1) in 22 h using a combination of soy peptone D (5 g/l) and corn steep liquor (50 g/l). This is the first report on the production of mannitol by fermentation using molasses and corn steep liquor.
Saha
Badal C
BC
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA. sahabc@ncaur.usda.gov
D019149:Bioreactors; D003362:Cost-Benefit Analysis; D003470:Culture Media / Q000191:economics*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000145:classification / Q000378:metabolism*; D008353:Mannitol / Q000378:metabolism*; D008965:Molasses
D003470:Culture Media; D019344:Lactic Acid; D008353:Mannitol