A low-cost medium for mannitol production by Lactobacillus intermedius NRRL B-3693.
The production of mannitol by Lactobacillus intermedius NNRL B-3693 using molasses as an inexpensive carbon source was evaluated. The bacterium produced mannitol (104 g/l) from molasses and fructose syrups (1:1; total sugars, 150 g/l; fructose:glucose 4:1) in 16 h. Several kinds of inexpensive organic and inorganic nitrogen sources and corn steep liquor were evaluated for their potential to replace more expensive nitrogen sources derived from Bacto-peptone and yeast extract. Soy peptone D (5 g/l) and corn steep liquor (50 g/l) were found to be suitable substitutes for Bacto-peptone (5 g/l) and Bacto-yeast extract (5 g/l), respectively. The bacterium produced 105 g mannitol per liter from the molasses and fructose syrup (1:1, total sugars 150 g/l; fructose:glucose 4:1) in 22 h using a combination of soy peptone D (5 g/l) and corn steep liquor (50 g/l). This is the first report on the production of mannitol by fermentation using molasses and corn steep liquor.
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA. sahabc@ncaur.usda.gov
Badal C
BC
Saha
D019149:Bioreactors; D003362:Cost-Benefit Analysis; D003470:Culture Media / Q000191:economics*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000145:classification / Q000378:metabolism*; D008353:Mannitol / Q000378:metabolism*; D008965:Molasses
D003470:Culture Media; D019344:Lactic Acid; D008353:Mannitol