Scatter factor mitigates HIV-1 gp120-induced human mesangial cell injury.
HIV-1 gp120 protein has been shown to promote mesangial cell (MC) injury. Scatter factor (SF) is a growth factor that plays a reparative role in various experimental models of renal lesions. We hypothesize that SF protects MC against HIV-1 gp120-induced MC injury. gp120 at a low dose, stimulated HMC proliferation (p < 0.0001). SF (50 ng/ml) further enhanced low-dose gp120-induced HMC proliferation. However, gp120 at higher doses (10-100 ng/ml) promoted HMC apoptosis. Nevertheless, SF attenuated the high-dose gp120-induced HMC apoptosis. Interestingly, gp120 at a low dose not only induced NF-kappaB activation but also increased p21(cip1/waf1) andp27(kip1) protein levels.
Department of Medicine, Long Island Jewish Medical Center (LIJMC), New Hyde Park, NY, USA.
Aditi A
AA
Kapasi
Saijun
S
Fan
Pravin C
PC
Singhal
D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015699:HIV Envelope Protein gp120 / Q000008:administration & dosage / Q000494:pharmacology*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D006801:Humans; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology* / Q000502:physiology; D016328:NF-kappa B / Q000378:metabolism
D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21; D015699:HIV Envelope Protein gp120; D016328:NF-kappa B; D050760:Cyclin-Dependent Kinase Inhibitor p27; D017228:Hepatocyte Growth Factor