Specific detection of gaseous NO and 15NO in the headspace from liquid-phase reactions involving NO-generating organic, inorganic, and biochemical samples using a mid-infrared laser.
Nitric oxide (NO) is an important biological signaling agent. The specific detection of NO represents a continuing challenge in the field of NO research. Many methods are currently employed for the detection of NO. Here, we report a qualitative but specific detection method for gaseous NO liberated in and from solution taking advantage of its low solubility. Importantly, our mid-infrared laser absorption method does not depend on any chemical derivatization of NO, and is applicable over a wide range of concentrations for both protein work and in organic-inorganic modeling work. We also apply this method to the specific detection of 15NO.
Yi
Jun
J
Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, OK 73019, USA.
Namjou
Khosrow
K
Zahran
Zaki N
ZN
McCann
Patrick J
PJ
Richter-Addo
George B
GB
D005740:Gases / Q000032:analysis*; D007834:Lasers; D009569:Nitric Oxide / Q000032:analysis*; D009587:Nitrogen Isotopes / Q000032:analysis; D012995:Solubility; D012996:Solutions / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods*
D005740:Gases; D009587:Nitrogen Isotopes; D012996:Solutions; D009569:Nitric Oxide