Reversal of diabetes in non-obese diabetic mice without spleen cell-derived beta cell regeneration.
Autoimmune destruction of beta cells is the predominant cause of type 1 diabetes mellitus (T1DM) in humans and is modeled in non-obese diabetic (NOD) mice. Many therapeutic interventions prevent the development of T1DM in NOD mice, but few can induce its reversal once established. Intervention with Freund's complete adjuvant, semi-allogeneic splenocytes, and temporary islet transplantation has been reported to cure NOD mice of established T1DM. Using the same approach, we report here that this treatment cured 32% of NOD mice of established diabetes (>340 milligrams per deciliter blood glucose), although beta cells in these mice were not derived from donor splenocytes.
Section of Transplantation, Department of Surgery, University of Chicago, Chicago, IL 60637, USA. achong@uchicago.edu
Anita S
AS
Chong
Jikun
J
Shen
Jing
J
Tao
Dengping
D
Yin
Andrey
A
Kuznetsov
Manami
M
Hara
Louis H
LH
Philipson
D000818:Animals; D015551:Autoimmunity; D001786:Blood Glucose / Q000032:analysis; D002454:Cell Differentiation; D017690:Cell Transplantation; D003131:Combined Modality Therapy; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D005260:Female; D005620:Freund's Adjuvant / Q000627:therapeutic use*; D049452:Green Fluorescent Proteins / Q000032:analysis; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000502:physiology; D016381:Islets of Langerhans Transplantation; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008822:Mice, Transgenic; D012038:Regeneration; D013154:Spleen / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
D001786:Blood Glucose; D049452:Green Fluorescent Proteins; D005620:Freund's Adjuvant