Peroxisomal trans-2-enoyl-CoA reductase is involved in phytol degradation.
Phytol is a naturally occurring precursor of phytanic acid. The last step in the conversion of phytol to phytanoyl-CoA is the reduction of phytenoyl-CoA mediated by an, as yet, unidentified enzyme. A candidate for this reaction is a previously described peroxisomal trans-2-enoyl-CoA reductase (TER). To investigate this, human TER was expressed in E. coli as an MBP-fusion protein. The purified recombinant protein was shown to have high reductase activity towards trans-phytenoyl-CoA, but not towards the peroxisomal beta-oxidation intermediates C24:1-CoA and pristenoyl-CoA. In conclusion, our results show that human TER is responsible for the reduction of phytenoyl-CoA to phytanoyl-CoA in peroxisomes.
Laboratory Genetic Metabolic Diseases (F0-224), Department of Clinical Chemistry, Emma's Children's Hospital, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.
J
J
Gloerich
J P N
JP
Ruiter
D M
DM
van den Brink
R
R
Ofman
S
S
Ferdinandusse
R J A
RJ
Wanders
D003065:Coenzyme A / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D020675:Peroxisomes / Q000201:enzymology*; D010831:Phytanic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D010836:Phytol / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
D011993:Recombinant Fusion Proteins; C089917:phytanoyl-coenzyme A; D010831:Phytanic Acid; D010836:Phytol; D044925:Oxidoreductases Acting on CH-CH Group Donors; C434934:trans-2-enoyl-CoA reductase (NADPH); D009247:NADH, NADPH Oxidoreductases; D003065:Coenzyme A