Adhesion of Lactobacillus plantarum 423 and Lactobacillus salivarius 241 to the intestinal tract of piglets, as recorded with fluorescent in situ hybridization (FISH), and production of plantaricin 423 by cells colonized to the ileum.
OBJECTIVE
To determine which intestinal section of pre and postweaned piglets are colonized by Lactobacillus plantarum 423 and Lactobacillus salivarius 241, and follow production of plantaricin 423 in a gastro-intestinal model.


RESULTS
Lactobacillus plantarum 423 and Lact. salivarius 241, single or in combination, were administered to 1-, 14- and 28-day-old (postweaned) piglets. According to results obtained by fluorescent in situ hybridization (FISH), Lact. plantarum 423 adhered strongly to the ileum and posterior colon and Lact. salivarius 241 to the duodenum in preweaned piglets. High numbers of strain 241 were recorded in the duodenum and posterior colon of postweaned piglets, whereas strain 423 remained localized to the ileum. Lowering in Enterococcus faecalis cell numbers were recorded when preweaned piglets were challenged with strain 241. Plantaricin 423 was produced for 96 h in the ileum section of a gastro-intestinal model.


CONCLUSIONS
Lactobacillus plantarum 423 and Lact. salivarius 241 adhere to different sections of the intestinal tract, depending on the piglet's age. Ent. faecalis were inhibited in vivo, probably by plantaricin 423.


CONCLUSIONS
Fluorescent in situ hybridization proved valuable in the detection of probiotic bacteria adhered to the intestine. This is the first report of bacteriocin production in a model simulating the porcine gastro-intestinal tract.
Maré
L
L
Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa.
Wolfaardt
G M
GM
Dicks
L M T
LM
D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001422:Bacterial Adhesion / Q000502:physiology; D001430:Bacteriocins / Q000096:biosynthesis*; D003106:Colon / Q000382:microbiology; D015169:Colony Count, Microbial / Q000379:methods; D004386:Duodenum / Q000382:microbiology; D013293:Enterococcus faecalis / Q000302:isolation & purification; D041981:Gastrointestinal Tract / Q000382:microbiology*; D007082:Ileum / Q000382:microbiology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007583:Jejunum / Q000382:microbiology; D007778:Lactobacillus / Q000502:physiology*; D048191:Lactiplantibacillus plantarum / Q000502:physiology; D019936:Probiotics / Q000008:administration & dosage*; D013045:Species Specificity; D013552:Swine
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C471833:plantaricin 423