Cell-permeable and biocompatible polymeric nanoparticles for apoptosis imaging.
Here, we report for the first time cell-permeable and biocompatible polymeric nanoparticles consisting of a polymer conjugated to a near-infrared (NIR) fluorescence (Cy5.5)-linked effector caspase-specific peptide. The close spatial proximity of the NIR fluorochromes in polymeric nanoparticles results in an autoquenched state, but polymer nanoparticles give rise to strong NIR fluorescence signal under apoptotic cells. Thus, the smart polymeric nanoparticle developed here is an attractive probe for real-time imaging of apoptosis in single cells.
Kim
Kwangmeyung
K
Biomedical Research Center, Korea Institute of Science and Technology, 39-1 Haweolgog-Dong, Sungbook-Gu, Seoul 136-791, Korea.
Lee
Misu
M
Park
Hyungkyu
H
Kim
Jong-Ho
JH
Kim
Sungwon
S
Chung
Hesson
H
Choi
Kuiwon
K
Kim
In-San
IS
Seong
Baik L
BL
Kwon
Ick Chan
IC
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics*; D002232:Carbocyanines / Q000737:chemistry; D053148:Caspase 3; D053179:Caspase 7; D020169:Caspases / Q000378:metabolism; D002463:Cell Membrane Permeability; D003840:Deoxycholic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008562:Membrane Glycoproteins / Q000494:pharmacology; D049329:Nanostructures / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D011094:Polyethyleneimine / Q000737:chemistry / Q000493:pharmacokinetics; D012542:Scattering, Radiation; D019265:Spectroscopy, Near-Infrared; D013379:Substrate Specificity; D053221:TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D051017:Apoptosis Regulatory Proteins; D001672:Biocompatible Materials; C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D008562:Membrane Glycoproteins; D009842:Oligopeptides; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha; D003840:Deoxycholic Acid; D011094:Polyethyleneimine; C505407:CASP3 protein, human; C505520:CASP7 protein, human; D053148:Caspase 3; D053179:Caspase 7; D020169:Caspases