[Preparation and characterization of immunogen against microcystin LR].
OBJECTIVE
To prepare Microcystins-LR (MC-LR) conjugates with the carrier protein for developing a new immune assay for MC-LR.


METHODS
MC-LR was coupled to BSA or KLH by "activated ester" and "water-soluble carbodimide". Measure the combine ratio of MC-LR with KLH or BSA by the UV scanning or the Bradford's method.


RESULTS
The results are 9.1 and 9.4. New Zealand rabbits was immunized by MC-LR-KLH. The titer of the polyantibodies was over 25,600 measured by id ELISA.


CONCLUSIONS
MC-LR-KLH was proved the suitable immunogen against MC-LR.
Zhao
Xiao-lian
XL
Jiangsu Institute of Microbiology, Wuxi 214063, China.
Sun
Wei-rong
WR
Sun
Xiu-lan
XL
Tang
Jian
J
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001427:Bacterial Toxins / Q000032:analysis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D017979:Gold Colloid; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis* / Q000276:immunology*; D011817:Rabbits
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D017979:Gold Colloid; D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR