Selection and Characterization of Aspartokinase Feedback-Insensitive Mutants of Azotobacter vinelandii.
Aspartokinase feedback-insensitive mutants of Azotobacter vinelandii were selected as resistant to l-threonine, (beta)-hydroxynorvaline, or S-(2-aminoethyl)-l-cysteine. l-Threonine-resistant strains were classified into three groups based on their ability to transport l-threonine and their growth response to O-methylthreonine and (beta)-hydroxynorvaline. Most of the mutants were transport defective; however, some were desensitized to feedback regulation.
Ekechukwu
C R
CR
Burns
T A
TA
Melton
T
T