Transcript profiles of Nitrosomonas europaea during growth and upon deprivation of ammonia and carbonate.
The transcriptome of Nitrosomonas europaea was analyzed with whole-genome microarrays. Growing cells were compared to cells deprived of (NH4)2SO4 and Na2CO3. Hybridization signals were detected for 76% of the genes represented on the array under either or both conditions. Transcript levels for 68% of the genes were at least twofold higher in growing cells than in deprived cells, while only 0.42% of the genes were present at more than twofold higher levels in deprived cells. Transcript levels for the remaining 7% of the genes did not change significantly with the treatments. These trends were confirmed for selected genes by Northern hybridizations and quantitative RT-PCR. Compared to heterotrophic bacteria, N. europaea downregulates a greater proportion of its genes and fewer genes appear to be associated with the adaptation to starvation.
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA.
Xueming
X
Wei
Tingfen
T
Yan
Norman G
NG
Hommes
Xiuduan
X
Liu
Liyou
L
Wu
Crystal
C
McAlvin
Martin G
MG
Klotz
Luis A
LA
Sayavedra-Soto
Jizhong
J
Zhou
Daniel J
DJ
Arp
D000641:Ammonia / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002254:Carbonates / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D018869:Heat-Shock Response; D043370:Nitrosomonas europaea / Q000254:growth & development* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002254:Carbonates; D000641:Ammonia