The effect of D,L-beta-hydroxybutyric acid on cell death and proliferation in L929 cells.
As a prerequisite for tissue engineering applications, researchers must understand the effect on local cell types of the degradation products of biodegradable polymers. Polyhydroxybutyrate (PHB) has received special interest as an implant material, because it degrades to release a normal component of blood and tissue, D,L-beta-hydroxybutyrate (HB). We report that HB (0.02 g/l) promoted cell proliferation in cultured L929 cells plated at high cell density (1 x 10(5) cells/well) but not lower cell densities. While HB did not affect cell cycle progression, it significantly inhibited cell death. HB treatment prevented necrosis, reducing cell membrane permeability 4h following serum withdrawal from the medium, and for all subsequent time points. In summary, HB promotes proliferation of L929 cells in high-density cultures by preventing apoptotic and necrotic cell death. This property makes biodegradable polymers containing HB, such as PHBHHx, attractive candidates for tissue engineering applications, especially those requiring the regeneration of large numbers of cells.
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Shan
S
Cheng
Guo-Qiang
GQ
Chen
Michael
M
Leski
Bing
B
Zou
Yang
Y
Wang
Qiong
Q
Wu
D020155:3-Hydroxybutyric Acid / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D051379:Mice; D011108:Polymers / Q000378:metabolism
D001672:Biocompatible Materials; D011108:Polymers; D020155:3-Hydroxybutyric Acid