[Effect of disc displacement on mRNA expression of urokinase plasminogen activator and its inhibitor-1 in synovial tissues].
OBJECTIVE
To investigate the effect of anterior disc displacement on the expression of urokinase plasminogen activator and its inhibitor-1 (uPA/PAI-1) in synovial tissues.


METHODS
Forty Japanese white rabbits were used in this study. The animals were killed at 4 days, 1, 2, 4, 8 and 12 weeks postoperatively, respectively. In situ hybridization technology was applied to detect the expression of uPA/PAI-1 mRNA in synovial membrane.


RESULTS
In normal synovial tissues, synovial lining cells and a few fibrosblasts with mild positive staining were occasionally seen. More synovial lining cells and fibrosblasts with moderate postive signals were found 1 week after operation. Since then, the degree of staining for uPA/PAI-1 increased gradually. By the end of 12 weeks postoperatively, strong signals of uPA/PAI-1 mRNA were detected.


CONCLUSIONS
There is a harmonized uPA/PAI-1 system existing in synovial tissues. The high expression of uPA and PAI-1 mRNA in synovial tissues indicates that the uPA/PAI-1 system may play an important role in the process of synovitis resulted from anterior disc displacement.
Zhan
Jing
J
Dept. of Oral and Maxillofacial Surgery, Hospital of Stomatology, Zhejiang University, Hangzhou 310006, China.
Wu
Li-Qun
LQ
Gu
Zhi-Yuan
ZY
Zhang
Yin-Kai
YK
Hu
Ji-An
JA
D000818:Animals; D017403:In Situ Hybridization; D010958:Plasminogen; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D011817:Rabbits; D013583:Synovial Membrane; D014568:Urokinase-Type Plasminogen Activator
D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D010958:Plasminogen; D014568:Urokinase-Type Plasminogen Activator