The value of autofluorescence as a diagnostic feature of acute promyelocytic leukemia.
Autofluorescence is an immunophenotypic characteristic of leukemic blasts in acute promyelocytic leukemia (APL). We examined the fluorescent intensity of isotype controls in 25 cases of APML and 25 controls with acute myeloid leukemia. The fluorescence of the FITC- and PE-conjugated controls was consistently higher in APML. Autofluorescence may therefore represent a helpful diagnostic marker in APML.
Patrick J
PJ
Hayden
Niamh M
NM
O'Connell
David A
DA
O'Brien
Paul
P
O'Rourke
Emer
E
Lawlor
Paul V
PV
Browne
D001752:Blast Crisis / Q000175:diagnosis / Q000473:pathology; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D005434:Flow Cytometry / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D016130:Immunophenotyping; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis* / Q000473:pathology