Fluorescent proteins as markers in the plant secretory pathway.
The use of fluorescent proteins and live cell imaging has greatly increased our knowledge of cell biology in recent years. Not only can these technologies be used to study protein trafficking under different conditions, but they have also been of use in elucidating the relationships between different organelles in a noninvasive manner. The use of multiple different fluorochromes allows the observation of interactions between organelles and between proteins, making this one of the fastest-developing and exciting fields at this time. In this review, we discuss the multitude of fluorescent markers that have been generated to study the plant secretory pathway. Although these markers have been used to solve many mysteries in this field, some areas that require further discussion remain.
Hanton
Sally L
SL
Department of Biology, 112 Science Place, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada.
Brandizzi
Federica
F
D029681:Arabidopsis Proteins / Q000502:physiology; D001426:Bacterial Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D008164:Luminescent Proteins / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000502:physiology; D009685:Nuclear Envelope / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism; D020696:rab5 GTP-Binding Proteins / Q000378:metabolism; D020931:ran GTP-Binding Protein / Q000502:physiology; D021601:trans-Golgi Network / Q000378:metabolism
D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; C485182:Cyan Fluorescent Protein; C507138:ERD2 protein, Arabidopsis; D008164:Luminescent Proteins; D008565:Membrane Proteins; C485192:green fluorescent protein, Aequorea victoria; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins; D020696:rab5 GTP-Binding Proteins; D020931:ran GTP-Binding Protein