Cloning and characterization of two EREBP transcription factors from cotton (Gossypium hirsutum L.).
In this research, two EREBP (ethylene response element binding protein) genes were isolated by the yeast one-hybrid system, named GhEREB2 and GhEREB3, and both have one intron in their coding regions. The deduced amino acid sequences of GhEREB2 and GhEREB3 have some typical features of transcription factors, one potential basic nuclear-localization signal, one possible acidic activation domain, and one conserved DNA binding domain, and they show high similarity, especially in the DNA-binding domain. GhEREB2 was expressed in roots, stems, and leaves (low levels), but GhEREB3 was only expressed in leaves. Furthermore, the expression of GhEREB2 and GhEREB3 was induced by ethylene and jasmonic acid. In addition, GhEREB2 and GhEREB3 proteins specifically bind to a GCC-box and strongly activate the expression of HIS3 and LacZ reporter genes in yeast. Collectively, these results suggest that GhEREB2 and GhEREB3 might be involved as positive transcription factors in biotic stress signal transduction pathways.
Duan
Hongying
H
College of Biological Sciences, China Agriculture University, Beijing.
Li
Fuguang
F
Wu
Xudong
X
Ma
Dongmei
D
Wang
Mao
M
Hou
Yuxia
Y
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D003368:Gossypium / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques
D004268:DNA-Binding Proteins; D010940:Plant Proteins; D020033:Protein Isoforms; C093680:ethylene-responsive element binding protein