Highly sensitive spectrofluorimetric determination of trace amounts of lecithin using a norfloxacin-terbium probe.
BACKGROUND
Lecithin is found throughout biological systems. A new spectrofluorimetric method was developed for the determination of trace amounts of lecithin.


METHODS
Optimum conditions for the determination of lecithin were investigated.


RESULTS
Using norfloxacin (NF)-terbium ion (Tb3+) as a fluorescent probe, in a buffer solution at pH=6.80, lecithin reduced the fluorescence intensity of the NF-Tb3+ complex at lambda=545 nm; the reduced fluorescence intensity of the Tb3+ ion is proportional to the concentration of lecithin. The linear range and detection limit for the determination of lecithin were 1.08 x 10(-6)-3.02 x 10(-5) mol/l and 2.54 x 10(-7) mol/l.


CONCLUSIONS
This method is simple, practical and relatively free of interference from coexisting substances, and can be successfully applied to assess lecithin in serum samples.
Bian
Weiwei
W
Department of Chemistry, Shandong Normal University, Jinan, 250014, P R China.
Jiang
Chongqiu
C
D002412:Cations / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009643:Norfloxacin / Q000097:blood* / Q000737:chemistry; D010713:Phosphatidylcholines / Q000096:biosynthesis / Q000097:blood* / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000097:blood* / Q000737:chemistry; D013997:Time Factors
D002412:Cations; D005456:Fluorescent Dyes; D010713:Phosphatidylcholines; D013725:Terbium; D009643:Norfloxacin