Papillomavirus E2 protein interacts with and stimulates human topoisomerase I.
The papillomavirus (PV) E2 protein plays a role in recruiting viral and cellular DNA replication factors, such as PV E1 or RPA to PV genomes. Using both purified proteins and through co-precipitation, it was determined that HPV-11 E2 binds human topoisomerase I. E2 can stimulate topoisomerase I DNA relaxation activity 3- to 4-fold. Conversely, topoisomerase I is unable to stimulate E2 DNA binding. These findings suggest that stimulation of topoisomerase I by E2 may help promote efficient relaxation of the torsional stress induced by PV DNA replication.
University at Buffalo, Departments of Microbiology and Immunology, Biochemistry and the Witebsky Center for Microbial Pathogenesis, 210 Biomedical Research Building, School of Medicine and Biomedical Sciences, Buffalo, NY 14214, USA.
Randolph V
RV
Clower
Yan
Y
Hu
Thomas
T
Melendy
D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D004797:Enzyme-Linked Immunosorbent Assay; D047468:Immunoprecipitation; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication / Q000502:physiology
D004279:DNA, Viral; C117355:E2 protein, Human papillomavirus type 11; D014764:Viral Proteins; D004264:DNA Topoisomerases, Type I