Passive air sampler as a tool for long-term air pollution monitoring: Part 2. Air genotoxic potency screening assessment.
The capability of passive air sampling to be employed in the evaluation of direct genotoxicity of ambient air samples was assessed. Genotoxic effects of the total extracts from the polyurethane foam filters exposed for 28 days during a regional passive air sampling campaign were investigated. Twenty sampling sites were selected in Brno city on the area of approximately 20x20 km in October and November 2004. Brno is the second largest city of the Czech Republic, highly industrialized with approximately 370,000 of permanent inhabitants. The levels of PAHs, PCBs, and chlorinated pesticides were determined in all samples. Fraction of each extract was also assayed in the bacterial genotoxicity test using Escherichia coli sulA::lacZ. Complete dose-response relationships of the air extracts were determined. The statistical analysis showed significant correlation between observed biological effects and PAHs concentrations in samples.
RECETOX-Research Center for Environmental Chemistry and Ecotoxicology, Masaryk University, Kamenice 3/126, 625 00 Brno, Czech Republic. cupr@recetox.muni.cz
Pavel
P
Cupr
Jana
J
Klánová
Tomás
T
Bartos
Zuzana
Z
Flegrová
Jirí
J
Kohoutek
Ivan
I
Holoubek
D000393:Air Pollutants / Q000032:analysis*; D002947:Cities; D018153:Czech Republic; D004249:DNA Damage; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D005240:Feasibility Studies; D006801:Humans; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D030781:Organisms, Genetically Modified; D011140:Polyurethanes
D000393:Air Pollutants; D009153:Mutagens; D011140:Polyurethanes; C028279:polyurethane foam