Molecular cloning and characterization of vitelline precursor protein B1 from Clonorchis sinensis.
In trematodes, vitelline precursor proteins are required for eggshell formation. A cDNA clone of Clonorchis sinensis (CsVpB1) was selected from an EST pool, encoding a polypeptide of 245 amino acids. The CsVpB1 polypeptide demonstrated homology with vitelline precursor proteins from trematodes with high sequential identities. In a phylogenic tree, CsVpB1 clustered with trematode VpB proteins. The CsVpB1 polypeptide was found to be rich in tyrosine residues, including putative predihydroxyphenyl alanine (DOPA) residues, involved in cross-linking of the precursor proteins. Mouse immune sera were raised against a recombinant CsVpB1 protein. In adult C. sinensis, CsVpB1 protein was exclusively localized in vitelline follicles. Based on these results, the CsVpB1 cDNA is believed to encode a VpB of C. sinensis.
Tang
Yi
Y
Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul, Republic of Korea.
Cho
Pyo Yun
PY
Kim
Bong Soo
BS
Hong
Sung-Jong
SJ
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D003003:Clonorchiasis / Q000175:diagnosis / Q000276:immunology; D003004:Clonorchis sinensis / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D018076:DNA, Complementary / Q000737:chemistry; D020224:Expressed Sequence Tags / Q000737:chemistry; D015723:Gene Library; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D047048:Vitellins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
D000947:Antigens, Helminth; D018076:DNA, Complementary; D015801:Helminth Proteins; D007106:Immune Sera; D011498:Protein Precursors; D011994:Recombinant Proteins; D047048:Vitellins