Combined effects of NaF and SLS on acid- and polysaccharide-formation of biofilm and planktonic cells.
Bacteria grow preferentially attached to surfaces embedded in an exopolysaccharide matrix to form biofilms. In this mode of growth, bacteria often show reduced susceptibility to antimicrobials. The aim of this study was to investigate whether the combination of NaF and the detergent sodium lauryl sulphate (SLS) could result in additive effects on acid formation by planktonic and biofilm cells, as well as on extracellular polysaccharide formation. An additive inhibitory effect on Streptococcus mutans acid formation was observed, both in planktonic and biofilm cells. In dental biofilms, SLS alone and in combination with NaF reduced acid formation. Extracellular polysaccharide formation by S. mutans and saliva was reduced by SLS alone and in combination with NaF. In dentifrices and mouthrinse solutions, NaF and SLS are often present in combination. It remains to be determined whether an additive effect on acid formation may also occur in dental biofilms under different concentrations from those used in the present study, and whether the effects may be selective for certain bacterial species.
Department of Oral Biology, Faculty of Dentistry, University of Oslo, N0316 Oslo, Norway. f.c.petersen@odont.uio.no
Fernanda C
FC
Petersen
Synnøve
S
Assev
Anne Aa
AA
Scheie
D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D001422:Bacterial Adhesion / Q000187:drug effects; D018407:Bacterial Physiological Phenomena; D018441:Biofilms; D003773:Dental Plaque / Q000382:microbiology / Q000517:prevention & control*; D004357:Drug Synergism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009067:Mouthwashes / Q000494:pharmacology*; D012463:Saliva / Q000378:metabolism / Q000382:microbiology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D012969:Sodium Fluoride / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects / Q000378:metabolism*; D013501:Surface-Active Agents / Q000494:pharmacology*
D009067:Mouthwashes; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D012969:Sodium Fluoride