[The study on the technique of sexing bovine pre-implantation embryos with two-temperature gradient multiplex PCR].
For the stable, reliable, fast method of sexing bovine pre-implantation embryos is still play very important role in husbandry production, the amplification experiment under the condition of the two-temperature gradient PCR was done with bovine samples such as genome, cloning embryos, embryos respectively. As a result, the stable, simple, fast method of two-temperature gradient PCR for sexing bovine pre-implantation embryos was obtained, which only took 57 minutes to identify the embryo sex. A total of 30 dairy embryos were sexed with the two-temperature gradient PCR method in the study. 15 identified embryos (11 female, 4 male) were transferred into 15 recipient cows, among them 7 were pregnant after 60 days. In the end, 5 female calves were aborted in late pregnancy, and 1 female and 1 male dairy calf were born. The sexes of aborted and born calves were fully in accordance with the embryo sex predetermination with PCR method.
Wang
Dong
D
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China. dongwang@iascaas.net.cn
Cheng
Jin-Hua
JH
Zhu
Hua-Bin
HB
Hao
Hai-Sheng
HS
Wang
Zong-Li
ZL
Yang
Bo
B
Wang
Zhen-Ling
ZL
Du
Wei-Hua
WH
Liu
Yong-Hua
YH
Zhang
Ying-Xia
YX
D000818:Animals; D002417:Cattle; D010064:Embryo Implantation / Q000235:genetics*; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D019849:Sex Determination Processes; D013696:Temperature