Knockdown of CD146 reduces the migration and proliferation of human endothelial cells.
Our previous study has demonstrated that CD146 molecule is a biomarker on vascular endothelium, which is involved in angiogenesis and tumor growth. However the mechanism behind is not clear. Here we have for the first time developed a novel CD146 blockade system using CD146 siRNA to study its function on endothelial cells. Our data showed that CD146 siRNA specifically blocked the expression of CD146 on both mRNA and protein levels, leading to the significant suppression of HUVEC proliferation, adhesion and migration. These results demonstrate that CD146 plays a key role in vascular endothelial cell activity and angiogenesis, and CD146 siRNA can be used as a new inhibitor for anti-angiogenesis therapy.
Kang
Yanyong
Y
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China.
Wang
Fengcai
F
Feng
Jing
J
Yang
Dongling
D
Yang
Xu
X
Yan
Xiyun
X
D051929:CD146 Antigen / Q000187:drug effects* / Q000235:genetics; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology; D005786:Gene Expression Regulation; D006801:Humans; D018919:Neovascularization, Physiologic; D012333:RNA, Messenger / Q000187:drug effects; D034741:RNA, Small Interfering / Q000494:pharmacology*; D014945:Wound Healing / Q000502:physiology
D051929:CD146 Antigen; D012333:RNA, Messenger; D034741:RNA, Small Interfering