An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro.
The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5'-GCGTCGGTTTCGGTGCTCAC-3') was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-gamma (IFN-gamma) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.
Takahashi
Noritoshi
N
Food Research and Development Laboratory, Morinaga Milk Industry Co., Ltd, Zama, Kanagawa, Japan. no_takah@morinagamilk.co.jp
Kitazawa
Haruki
H
Shimosato
Takeshi
T
Iwabuchi
Noriyuki
N
Xiao
Jin-Zhong
JZ
Iwatsuki
Keiji
K
Kokubo
Sadayuki
S
Saito
Tadao
T
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D001483:Base Sequence; D001644:Bifidobacterium / Q000235:genetics* / Q000276:immunology*; D049109:Cell Proliferation; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D019936:Probiotics / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D013047:Specific Pathogen-Free Organisms
D004269:DNA, Bacterial; D007132:Immunoglobulin Isotypes; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D018664:Interleukin-12; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma