[Expression of costimulator 4-1BBL and B7-1 on glioma cell lines].
OBJECTIVE
Costimulatory molecules 4-1BBL and B7-1 have been proposed to be therapeutic targets to improve and sustain antitumor immune response. This study was to investigate the expression of 4-1BBL and B7-1 on several glioma cell lines.


METHODS
The expression of 4-1BBL and B7-1 on glioma cell lines T98G, MGR2, MGR1, SF767, SKMG1, SKMG4, and UW28 was detected by flow cytometry. The cytotoxic effects of vincristine (VCR) on the glioma cell lines were assessed with MTT assay. The correlation of 4-1BBL expression to drug resistance was analyzed.


RESULTS
The 7 glioma cell lines showed various levels of 4-1BBL expression, but none expressed B7-1. The positive rate of 4-1BBL was above 30% in T98G and MGR1 cells which were resistant to VCR; while it was below 10% in MGR2, SF767, SKMG1, SKMG4, and UW28 cells which were sensitive to VCR.


CONCLUSIONS
All of the detected cell lines express 4-1BBL, but none expresses B7-1. The glioma cells with high 4-1BBL expression are resistant to VCR.
State Key Laboratory of Oncology in South China, Guangzhou, Guangdong 510060, P. R. China.
Yong-Gao
YG
Mu
Hui
H
Peng
Jun-Ying
JY
Zhang
Cui-Jie
CJ
Shao
Chang-You
CY
Wu
Zhong-Ping
ZP
Chen
D053330:4-1BB Ligand; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D018122:B7-1 Antigen / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects; D019008:Drug Resistance, Neoplasm; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008024:Ligands; D048069:Tumor Necrosis Factors / Q000378:metabolism*; D014750:Vincristine / Q000494:pharmacology
D053330:4-1BB Ligand; D000972:Antineoplastic Agents, Phytogenic; D018122:B7-1 Antigen; D008024:Ligands; C505974:TNFSF9 protein, human; D048069:Tumor Necrosis Factors; D014750:Vincristine