Comparison and problems of measured values of LH, FSH, and PRL among measurement systems.
Several measurement systems are used for LH, FSH, and PRL in Japan, but the comparison of the measured values is difficult in some cases. In this study, we compared the measured values of 3 types of measurement systems widely used in Japan, SPAC-S, ARCHITECT, and Centaur, in females with a normal menstrual cycle and with various ovulatory disorders. Variant LH was discriminated by the criterion, SPAC-S LH/ARCHITECT LH ratio <0.5. Excluding the variant LH, the correlation of the measured LH values among the measurement systems was high, 0.94-0.99, and the value measured by ARCHITECT LH and Centaur LH was slightly higher than that measured by SPAC-S LH. As for FSH, the correlation was also high, 0.94-1.00, and the value measured by ARCHITECT FSH and Centaur FSH was slightly lower than that measured by SPAC-S FSH. The important judgment criterion of polycystic ovary syndrome (PCOS), a high level of LH relative to FSH, has been set to 1.0 or higher LH/FSH using SPAC-S. When the regression line of the LH/FSH ratio among the measurement systems was investigated, it can be said that 1.25 or higher by ARCHITECT and 1.40 or higher by Centaur are appropriate for the judgment criteria of a high level of LH relative to FSH. As for PRL, the correlation was high, 0.96-0.99, but the value showed large differences among the systems. For the diagnosis of hyperprolactinemia, the higher limit of the normal range may be 15 ng/mL for SPAC-S, 30 ng/mL for ARCHITECT, and 24 ng/mL for Centaur.
Department of Obstetrics and Gynecology, University of Tokushima School of Medicine, Japan.
Takeshi
T
Iwasa
Toshiya
T
Matsuzaki
Naoko
N
Tanaka
Masahiro
M
Minakuchi
Fumi
F
Shimizu
Rie
R
Ogata
Machiko
M
Kiyokawa
Masahiko
M
Maegawa
Toshiyuki
T
Yasui
Akira
A
Kuwahara
Minoru
M
Irahara
D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D006801:Humans; D006966:Hyperprolactinemia / Q000097:blood / Q000175:diagnosis; D015592:Immunoradiometric Assay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D007986:Luteinizing Hormone / Q000097:blood*; D008875:Middle Aged; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000175:diagnosis; D011247:Pregnancy; D011388:Prolactin / Q000097:blood*; D012015:Reference Standards; D012044:Regression Analysis
D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone