Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling.
Sex-specific isolation of cells from mixtures would greatly facilitate forensic casework. Thus, male and female cell mixtures were marked with a fluorescent X/Y-probe CEP X SpectrumOrange/Y SpectrumGreen DNA probe kit for fluorescence in situ hybridization, and single cells were isolated via laser microdissection (LMD). DNA profiling of LMD isolated, hybridized cells showed usable short tandem repeat profiles for at least 20 cells, which are comparable with results from other studies. To simulate casework samples, the method was also optimized for air-dried samples.
Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7a, 80337, Munich, Germany. katja.anslinger@med.uni-muenchen.de
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Anslinger
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Bayer
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Mack
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Eisenmenger
D001773:Blood Cells; D001801:Blood Stains; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D042282:Microdissection / Q000379:methods*; D018613:Microscopy, Confocal; D012727:Sex Characteristics; D012732:Sex Determination Analysis / Q000379:methods*
D005456:Fluorescent Dyes