Intra-articular enzyme administration for joint disease in feline mucopolysaccharidosis VI: enzyme dose and interval.
Degenerative joint changes have been reported in human mucopolysaccharidosis VI (MPS VI) and are a prominent feature of feline MPS VI. Joint disease has proven refractory to intravenous enzyme replacement therapy (ERT) in the MPS VI cat because enzyme is unable to reach cells in cartilage. In this study, enzyme was infused directly into the intraarticular space to determine whether joint tissues are able to respond to replacement enzyme. Clearance of glycosaminoglycans from chondrocytes was observed at a dose of 10 microg recombinant human N-acetylgalactosamine-4-sulfatase (rh4S), but greater clearance was observed with higher doses. The chondrocytes at the articular surface were cleared preferentially. Lysosomal vacuolation in cruciate ligament and synovial cells also decreased upon addition of rh4S. One month after injection of rh4S, a slight reaccumulation of storage was observed at the surface of the joint, but extensive reaccumulation was observed 2 mo after injection. These results indicate that by bypassing the synovium using intraarticular ERT, significant reduction in storage material in joint tissues can be achieved. Localized ERT in the joint space provides a mechanism for delivering enzyme directly to the articular cartilage and a potential therapy for joint pathology in MPS VI.
Lysosomal Diseases Research Unit, Department of Genetic Medicine, Children, Youth and Women's Health Service, North Adelaide, Australia.
Dyane
D
Auclair
Leanne K
LK
Hein
John J
JJ
Hopwood
Sharon
S
Byers
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002415:Cats; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D007592:Joint Diseases / Q000209:etiology / Q000473:pathology / Q000628:therapy / Q000662:veterinary; D007596:Joints / Q000033:anatomy & histology / Q000473:pathology; D008297:Male; D009087:Mucopolysaccharidosis VI / Q000150:complications / Q000473:pathology / Q000628:therapy / Q000662:veterinary; D020051:N-Acetylgalactosamine-4-Sulfatase / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use; D013583:Synovial Membrane / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D011994:Recombinant Proteins; D020051:N-Acetylgalactosamine-4-Sulfatase