[Evaluation of a new fluorometric immunoassay for the detection of anti-cyclic citrullinated peptides autoantibodies in rheumatoid arthritis].
Anti-cyclic citrullinated antibodies occurrence is a recent serological marker for rheumatoid arthritis. The aim of our study was to evaluate the measurement of these antibodies by a new fluorescent-enzyme immunoassay, called EliA CCP, fully automated onto UniCAP 100. This evaluation reveals correct and shows a within run imprecision of 4.6 to 10.5 % and a between-assay imprecision of 9,5 %. The comparison with an Elisa method (Euroimmun) shows a good correlation of anti-CCP concentrations without any major discrepancy.
Laboratoire d'Immunologie et Allergologie, CHU Angers.
F
F
Dubois-Galopin
C
C
Masson
A
A
Chevailler
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000175:diagnosis*; D001323:Autoantibodies / Q000097:blood*; D005260:Female; D005470:Fluorometry; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D008875:Middle Aged; D010456:Peptides, Cyclic / Q000276:immunology*; D015203:Reproducibility of Results
D001323:Autoantibodies; D010456:Peptides, Cyclic; C487763:cyclic citrullinated peptide