In vivo induction of mucosal immune responses by intranasal administration of chitosan microspheres containing Bordetella bronchiseptica DNT.
In vitro immune-stimulating activities of Bordetella bronchiseptica dermonecrotoxin (BBD)-loaded in chitosan microspheres (CMs) were reported with a mouse alveolar macrophage cell line (RAW264.7). Based on the report, in vivo activity of immune-induction was investigated by intranasal administration of the BBD-loaded CMs into mice. BBD was loaded into the CMs prepared by an ionic gelation process with tripolyphosphate. Mice were immunized by direct administration of the BBD-loaded CMs into the nasal cavity. After immunization of the mice, BBD-specific immune responses (IgG and IgA titers) were measured in sera, nasal wash, and saliva by ELISA. BBD-specific IgA titers in the nasal cavity were time- and dose-dependently increased by the administration. Similar phenomena were observed in the analysis of systemic IgA and IgG in sera. However, the antibody in saliva was undetectable by ELISA. These results suggested that direct vaccination via the nasal cavity was effective for targeting nasal-associated lymphoid tissues, and that CMs were an efficient adjuvant in nasal mucosal immunity for atrophic rhinitis vaccine.
Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Mi Lan
ML
Kang
Sang Gyun
SG
Kang
Hu-Lin
HL
Jiang
Seung Won
SW
Shin
Deog Yong
DY
Lee
Jeong-Min
JM
Ahn
Nabin
N
Rayamahji
In-Kyu
IK
Park
Sung Jae
SJ
Shin
Chong-Su
CS
Cho
Han Sang
HS
Yoo
D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D016950:Bordetella bronchiseptica / Q000276:immunology; D002460:Cell Line; D048271:Chitosan / Q000008:administration & dosage*; D004797:Enzyme-Linked Immunosorbent Assay; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D012463:Saliva / Q000276:immunology
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D048271:Chitosan