Bioluminescence imaging of live infected salmonids reveals that the fin bases are the major portal of entry for Novirhabdovirus.
Although Novirhabdovirus viruses, like the Infectious hematopietic necrosis virus (IHNV), have been extensively studied, limited knowledge exists on the route of IHNV entry during natural infection. A recombinant IHNV (rIHNV) expressing the Renilla luciferase gene was generated and used to infect trout. A noninvasive bioluminescence assay was developed so that virus replication in live fish could be followed hours after infection. We provide here evidence that the fin bases are the portal of entry into the fish. Confirmation was brought by the use of a nonpathogenic rIHNV, which was shown to persist in fins for 3 weeks postinfection.
Unité de Virologie et Immunologie Moléculaires, INRA, CRJ Domaine de Vilvert, 78352 Jouy en Josas, France.
Abdallah
A
Harmache
Monique
M
LeBerre
Stéphanie
S
Droineau
Marco
M
Giovannini
Michel
M
Brémont
D000818:Animals; D004274:DNA, Recombinant / Q000235:genetics; D005121:Extremities / Q000033:anatomy & histology / Q000502:physiology*; D005393:Fish Diseases / Q000503:physiopathology / Q000821:virology; D017930:Genes, Reporter; D016679:Genome, Viral; D007091:Image Processing, Computer-Assisted; D029142:Infectious hematopoietic necrosis virus / Q000235:genetics / Q000472:pathogenicity*; D007700:Kinetics; D008099:Liver / Q000378:metabolism / Q000821:virology; D008156:Luciferases / Q000378:metabolism; D049449:Luminescence; D029141:Novirhabdovirus / Q000502:physiology*; D017686:Oncorhynchus mykiss / Q000033:anatomy & histology* / Q000821:virology*; D018353:Rhabdoviridae Infections / Q000401:mortality / Q000503:physiopathology* / Q000662:veterinary / Q000821:virology; D013154:Spleen / Q000378:metabolism / Q000821:virology; D014779:Virus Replication
D004274:DNA, Recombinant; D008156:Luciferases