Semibiological molecular machine with an implemented "AND" logic gate for regulation of protein folding.
A semibiological molecular machine with an implemented "AND" logic gate was developed, which was capable of controlling the folding process of proteins in response to ATP and light as input stimuli. The molecular design made use of a genetically engineered chaperonin GroEL bearing, at both entrance parts of its cylindrical cavity, cysteine residues, which were functionalized by an azobenzene derivative to construct photoresponsive mechanical gates (azo-GroEL). This engineered chaperonin trapped denatured green fluorescent protein (GFP(denat)) and prohibited its refolding. However, when hosting azo-GroEL detected ATP (input stimulus 1) and UV light (input stimulus 2) at the same time, it quickly released GFP(denat) to allow its refolding. In contrast, reception of either input stimulus 1 or 2 resulted in only very slow or no substantial refolding of GFP(denat). Implementation of such "AND" logic gate mechanisms in mechanically driven biomolecular systems is an important step toward the design of secured drug delivery systems.
Muramatsu
Shinichi
S
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Kinbara
Kazushi
K
Taguchi
Hideki
H
Ishii
Noriyuki
N
Aida
Takuzo
T
D000251:Adenosine Triphosphatases / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry* / Q000528:radiation effects; D018834:Chaperonin 60 / Q000737:chemistry* / Q000528:radiation effects; D002942:Circular Dichroism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000528:radiation effects; D008128:Logic; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000528:radiation effects; D010777:Photochemistry; D017510:Protein Folding; D013056:Spectrophotometry, Ultraviolet
D001391:Azo Compounds; D018834:Chaperonin 60; D020409:Molecular Motor Proteins; D049452:Green Fluorescent Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C009850:azobenzene