Intergeniculate leaflet: contributions to photic and non-photic responsiveness of the hamster circadian system.
The circadian visual system is able to integrate light energy over time, enabling phase response and Fos induction in the suprachiasmatic nucleus to increase in proportion to the total energy of the photic stimulus. In the present studies, the contribution of the intergeniculate leaflet to light energy integration by the hamster circadian rhythm system was evaluated. Fos protein is induced in intergeniculate leaflet neurons at much lower irradiance levels than seen in suprachiasmatic nucleus neurons. Bilateral N-methyl-d-aspartate lesions of the intergeniculate leaflet decreased phase response of the circadian locomotor rhythm to high irradiance and, in animals exposed to long duration light stimuli, reduced Fos induction in the suprachiasmatic nucleus. Normal photon integration, as indicated by attenuated rhythm phase shifts and Fos induction in suprachiasmatic nucleus cells in response to the energy in light stimuli, does not occur in the absence of the intergeniculate leaflet and is likely to be a property of the circadian rhythm system, rather than solely of the suprachiasmatic nucleus. Anatomical analysis showed that virtually no intergeniculate leaflet neurons projecting to the suprachiasmatic nucleus contain Fos induced by either light or locomotion in a novel wheel. However, cells projecting to the pretectum were found to contain novel-wheel induced Fos. The intergeniculate leaflet is implicated in the normal assessment of light by the circadian rhythm system, but the circuitry by which either photic or non-photic information gains access to the suprachiasmatic nucleus may be more complex than previously thought.
Graduate Program in Neuroscience, Stony Brook University, Stony Brook, NY 10016, USA.
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D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count / Q000379:methods; D002772:Cholera Toxin / Q000008:administration & dosage / Q000378:metabolism; D002940:Circadian Rhythm / Q000502:physiology* / Q000528:radiation effects; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity; D005455:Fluorescent Antibody Technique / Q000379:methods; D007839:Functional Laterality / Q000502:physiology; D005829:Geniculate Bodies / Q000166:cytology / Q000293:injuries / Q000502:physiology*; D008297:Male; D016202:N-Methylaspartate / Q000633:toxicity; D009475:Neurons, Afferent / Q000502:physiology / Q000528:radiation effects; D016761:Oncogene Proteins v-fos / Q000378:metabolism; D010775:Photic Stimulation / Q000379:methods; D012420:Running / Q000502:physiology; D013493:Suprachiasmatic Nucleus / Q000378:metabolism* / Q000528:radiation effects; D014795:Visual Pathways / Q000502:physiology*
D018690:Excitatory Amino Acid Agonists; D016761:Oncogene Proteins v-fos; D016202:N-Methylaspartate; D002772:Cholera Toxin