Enhancement of DNA vaccine potency through linkage of antigen to filamentous bacteriophage coat protein III domain I.
Although DNA-based cancer vaccines have been successfully tested in mouse models, a major drawback of cancer vaccination still remains, namely that tumour antigens are weak and fail to generate a vigorous immune response in tumour-bearing patients. Genetic technology offers strategies for promoting immune pathways by adding immune-activating genes to the tumour antigen sequence. In this work, we converted a model non-immunogenic antigen into a vaccine by fusing it to domain I of the filamentous bacteriophage coat protein III gene. Vaccination with a DNA construct encoding the domain I fusion generated antigen-specific T helper 1-type cellular immune responses. These results demonstrate that the incorporation of protein III into a DNA vaccine formulation can modulate the gene-mediated immune response and may thus provide a strategy for improving its therapeutic effect.
Servicio de Inmunología, Hospital Universitario Puerta de Hierro, Madrid, Spain.
Angel M
AM
Cuesta
Eduardo
E
Suárez
Martin
M
Larsen
Kim Bak
KB
Jensen
Laura
L
Sanz
Marta
M
Compte
Peter
P
Kristensen
Luis
L
Alvarez-Vallina
D000276:Adjuvants, Immunologic; D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology*; D036022:Capsid Proteins; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood; D004268:DNA-Binding Proteins / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000096:biosynthesis; D007135:Immunoglobulin Variable Region / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology
D000276:Adjuvants, Immunologic; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D036022:Capsid Proteins; D016207:Cytokines; D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D007135:Immunoglobulin Variable Region; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D014760:Viral Fusion Proteins