Modulation of mitogen-activated protein kinases (MAPK) activity in response to different immune stimuli in haemocytes of the common periwinkle Littorina littorea.
The modulation of mitogen-activated protein kinase (MAPK) activity in haemocytes of the common periwinkle (Littorina littorea) in response to immune challenges by lipopolysaccharide from Echerichia coli (LPS), mannan from baker's yeast Saccharomyces cerevisiae and secretory-excretory products (SEP) of trematodes Himasthla elongata (Echinostomatidae) or after the treatment with phorbol ester (PMA) has been studied by Western blotting using affinity purified rabbit polyclonal antibodies. Exposure of the cells in suspension to PMA, LPS and mannan triggered an activation of p38 and ERK2. The JNK-mediated cascade was modulated differently by the elicitors examined. PMA treatment caused a transient activation of the JNK54 isoform, LPS exposure resulted in a decrease in activity of JNK46, and mannan had no effect on JNK phosphorylation status. Incubation of periwinkle haemocytes in culture medium containing trematode SEP did not affect the activity of any MAPK.
Laboratory 13, Institute of Evolutionary Physiology and Biochemistry RAS, 194223, pr. Torez 44, Saint-Petersburg, Russia. nyakovleva@gmail.com
Nadya V
NV
Iakovleva
Alexander M
AM
Gorbushin
Kenneth B
KB
Storey
D000595:Amino Acid Sequence; D000818:Animals; D004926:Escherichia coli / Q000737:chemistry; D048049:Extracellular Signal-Regulated MAP Kinases / Q000096:biosynthesis / Q000187:drug effects; D006434:Hemocytes / Q000276:immunology; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000096:biosynthesis / Q000187:drug effects; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D008351:Mannans / Q000276:immunology / Q000494:pharmacology*; D020928:Mitogen-Activated Protein Kinases / Q000187:drug effects* / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D016415:Sequence Alignment; D012908:Snails / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014200:Trematoda / Q000737:chemistry* / Q000276:immunology; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis / Q000187:drug effects
D008070:Lipopolysaccharides; D008351:Mannans; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D013755:Tetradecanoylphorbol Acetate