Transient permeability induced by alkyl derivatives of amphotericin B in lipid membranes.
Individual ionic channels were shown to be formed in the brain cholesterol containing phospholipid membranes by two-sided addition of the amphotericin B alkyl derivatives. At concentrations between 10(-8) and 10(-7) M, the resulting conductance appeared to be transient. Existence of different antibiotic assemblies was justified by the kinetic analysis of the membrane conductance decline following the antibiotic washing out. In order to account for the transient characteristics of the induced conductance, it was proposed that the antibiotic oligomers incorporate into the membrane from the aqueous phase, form channels aggregating with cholesterol, and then dissociate in the bilayer into non-active degraded oligomeric or monomeric forms.
Department of Biophysics, Baku State University, Z. Khalilov str., 23 AZ-1148 Baku, Azerbaijan.
Vafa
V
Ibragimova
Irada
I
Alieva
Khalil
K
Kasumov
Vitaly
V
Khutorsky
D000666:Amphotericin B / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D004563:Electrochemistry; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D008051:Lipid Bilayers; D000666:Amphotericin B