Inhibition of ganciclovir-resistant human cytomegalovirus replication by Kampo (Japanese herbal medicine).
We examined the effect of Kampo on the replication of ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) in the human embryonic fibroblast cell line MRC-5. Treatment of HCMV-infected cells with Sho-seiryu-to (SST; Xiao-Qing-Long-Tang in Chinese) resulted in the inhibition of viral replication without affecting the cell growth. SST treatment decreased the synthesis of viral DNA, but had no virucidal effect on cell-free HCMV. However, the inhibitory effect of SST on HCMV replication was ablated by anti-interferon-beta (IFN-beta) antibody suggesting that SST inhibits the replication of GCV-resistant HCMV through the induction of IFN-beta. These results suggest that SST is a novel compund with potential as an anti-HCMV.
Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan. t-murayama@hokuriku-u.ac.jp
Tsugiya
T
Murayama
Nobuo
N
Yamaguchi
Kozo
K
Iwamoto
Yoshito
Y
Eizuru
D000998:Antiviral Agents / Q000494:pharmacology*; D003587:Cytomegalovirus / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000188:drug therapy / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D024882:Drug Resistance, Viral; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts; D015774:Ganciclovir / Q000494:pharmacology*; D006801:Humans; D016899:Interferon-beta / Q000378:metabolism; D020835:Medicine, Kampo; D009693:Nucleic Acid Hybridization; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D004279:DNA, Viral; D004365:Drugs, Chinese Herbal; D016899:Interferon-beta; D015774:Ganciclovir