Does mRNA level of microsomal carnitine palmitoyltransferase predict yield of peripheral blood stem cell apheresis?
Transplantation of autologous hematopoietic stem cells is a well established therapeutic procedure. Despite advances in efficacy of the stem cell mobilization and apheresis process until now a predictive factor for the expected stem cell yield before initiation of mobilization therapy could not be identified. The main objective of our study was to evaluate alterations in enzymes involved in fatty acid metabolism on the level of gene expression in mononuclear cells, as changes in relative mRNA levels of these enzymes could represent the hematopoietic regenerative potential. Data of 23 consecutive patients with different lymphoid malignancies undergoing stem cell mobilization were analyzed. Our results show that mRNA levels of microsomal carnitine palmitoyltransferase in peripheral blood mononuclear cells quantified before application of mobilization therapy correlate positively with the amount of CD34 positive cells in peripheral blood before first apheresis, in the first apheresis product and in the total harvest outcome. The association of enzymes involved in fatty acid metabolism with hematoopoiesis was further confirmed in healthy subjects on altitude-adaptation training and in proliferating or differentiating HL60 cells. This gives evidence for a possible predictive value of such analyzes though further data of a larger sample are to be collected to confirm our observations.
3rd Department of Medicine, Hanusch Hospital, H. Collinstrasse 30, 1140 Vienna, Austria.
Michael
M
Fillitz
Heidrun
H
Karlic
Heinz
H
Tüchler
Johannes
J
Zeibig
Wolfgang
W
Spiegel
Peter
P
Wihlidal
Franz
F
Varga
Elisabeth
E
Pittermann
Michael
M
Pfeilstöcker
D000293:Adolescent; D000328:Adult; D000531:Altitude; D018952:Antigens, CD34 / Q000097:blood; D001781:Blood Component Removal; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics*; D003114:Colony-Forming Units Assay; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000032:analysis; D019204:GTP-Binding Proteins / Q000032:analysis; D018922:HL-60 Cells; D019337:Hematologic Neoplasms / Q000097:blood / Q000473:pathology; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D020962:Heterotrimeric GTP-Binding Proteins / Q000032:analysis; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D008875:Middle Aged; D027701:Organic Cation Transport Proteins / Q000032:analysis; D036102:Peripheral Blood Stem Cell Transplantation; D012333:RNA, Messenger / Q000097:blood* / Q000235:genetics; D000074058:Solute Carrier Family 22 Member 5; D014182:Transplantation, Autologous
D018952:Antigens, CD34; D027701:Organic Cation Transport Proteins; D012333:RNA, Messenger; C492020:SLC22A5 protein, human; D000074058:Solute Carrier Family 22 Member 5; D002334:Carnitine O-Palmitoyltransferase; D019204:GTP-Binding Proteins; C112631:G protein alpha 16; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D020962:Heterotrimeric GTP-Binding Proteins