A comparative study of upright counter-current chromatography and high-performance liquid chromatograpohy for preparative isolation and purification ofphenolic compounds from Magnoliae officinalis.
A comparative study of preparative isolation and purification of the phenolic compounds magnolol and honokiol from the Chinese medicinal plant Magnoliae officinalis by upright counter-current chromatography (CCC) and semi-preparative HPLC is presented. The comparison reveals that with a two-phase solvent system composed of light petroleum (bp 60-90 degrees C)-ethyl acetate-tetrachloromethane-methanol-water (1:1:8:6:1, v/v), 1250 mg of honokiol and 520 mg of magnolol, with a purity of 98.7 and 99.5%, respectively, were obtained from 2.0 g of a crude sample of Magnoliae officinalis in a single CCC separation. In contrast, semi-preparative HPLC allowed isolation and purification of these two phenolic compounds with significantly lower productivity and higher solvent consumption. Structures of the purified compounds were identified by 1H and 13C NMR.
Lu
Yanbin
Y
Department of Chemistry, Zhejiang University, Hangzhou, China.
Sun
Cuirong
C
Pan
Yuanjiang
Y
D001713:Biphenyl Compounds / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods*; D017705:Lignans / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D031566:Magnolia / Q000737:chemistry*; D015394:Molecular Structure; D012997:Solvents; D000498:Allyl Compounds; D010636:Phenols
D001713:Biphenyl Compounds; D017705:Lignans; D012997:Solvents; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols