Amplified immunohistochemical detection of PrPsc in animal transmissible spongiform encephalopathies using streptomycin.
Due to its sensitivity, immunohistochemistry (IHC) of abnormal prion protein (PrPsc) is used to study experimental and natural cases of transmissible spongiform encephalopathies (TSEs) such as Creutzfeldt-Jakob disease in humans or scrapie and bovine spongiform encephalopathy (BSE) in animals. The limits of detection are particularly critical when PrPsc IHC is used for diagnostic purposes. In this article, we describe for the first time the use of streptomycin sulfate in IHC, providing a novel original and easy way to amplify specifically PrPsc immunohistochemical detection in natural cases of BSE and scrapie, as well as in experimental TSEs in mice models using two different PrP antibodies.
Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, Unité ATNC, Lyon, Cedex 07-France. a.bencsik@lyon.afssa.fr
Anna A
AA
Bencsik
Anthony W
AW
Coleman
Sabine O S
SO
Debeer
Hervé
H
Perron
Aly
A
Moussa
D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D007202:Indicators and Reagents; D051379:Mice; D008810:Mice, Inbred C57BL; D018621:PrPC Proteins / Q000378:metabolism*; D012608:Scrapie / Q000378:metabolism*; D012756:Sheep; D013307:Streptomycin
D007202:Indicators and Reagents; D018621:PrPC Proteins; D013307:Streptomycin