The sperm chromatin structure assay as a diagnostic tool in the human fertility clinic.
BACKGROUND
Sperm DNA integrity has been shown to be necessary for achieving and sustaining embryo development. The objective was to evaluate the sperm chromatin structure assay (SCSA) as a diagnostic tool in clinical practice for intrauterine insemination (IUI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatments.


METHODS
A total of 385 semen samples from 234 couples were frozen for SCSA, and smears were prepared for morphology: 48 IUI, 139 IVF and 47 ICSI. The main SCSA variables were DNA fragmentation index (DFI), standard deviation of DFI (SD-DFI) and high DNA stainability (HDS), and the reproductive outcomes were biochemical pregnancy (BP), clinical pregnancy (CP) and implantation ratio (IR).


RESULTS
The results showed no significant difference in the fertility variables BP, CP and IR when <27% DFI was used between the IVF and ICSI groups. A low number of patients received IUI with low success rate, and statistical analysis was therefore not performed. Ongoing pregnancy was achieved for both IVF and ICSI couples with DFI levels >27%, and six couples in ICSI treatment achieved CP full-term. DFI >27% had a high prognostic power for predicting no CP for IVF patients, with a specificity of 97%. Couples diagnosed with male infertility had a significantly higher level of DFI compared to couples with idiopathic fertility. Sperm head morphology showed low but significant correlations with the SCSA variables.


CONCLUSIONS
SCSA is a useful tool in andrological diagnosis and contributes with a prognosis for the fertility outcome of conventional IVF. Although full-term pregnancy can be achieved with assisted reproductive techniques with a DFI >27%, the probability of a successful pregnancy may be reduced.
Department of Large Animal Sciences, Section for Veterinary Reproduction & Obstetrics, The Royal Veterinary & Agricultural University, Frederiksberg C, Denmark. gbh@kvl.dk
Gry Brandt
GB
Boe-Hansen
Jens
J
Fedder
Annette Kjaer
AK
Ersbøll
Preben
P
Christensen
D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis*; D007315:Insemination, Artificial / Q000379:methods; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D013094:Spermatozoa / Q000378:metabolism; D016896:Treatment Outcome
D002843:Chromatin; D004247:DNA