Inhibitory effect of Patrinia scabiosaefolia on acute pancreatitis.
OBJECTIVE
To investigate the effect of Patrinia scabiosaefolia (PS) on the cholecystokinin (CCK) octapeptide-induced acute pancreatitis (AP) in rats.


METHODS
Wistar rats weighing 240-260 g were divided into three groups: (1) Normal saline-treated group; (2) treatment with PS at 100 mg/kg group, in which PS was administered orally, followed by subcutaneous administration of 75 microg/kg CCK octapeptide three times after 1, 3 and 5 h, and this whole procedure was repeated for 5 d; (3) treatment with saline group, in which the protocols were the same as in treatment group with PS. We determined the pancreatic weight/body weight ratio, the levels of pancreatic HSP60, HSP72 and the secretion of pro-inflammatory cytokines. Repeated CCK octapeptide treatment resulted in the typical laboratory findings of experimentally induced pancreatitis.


RESULTS
PS reduced the pancreatic weight/body weight ratio, the levels of serum amylase and lipase, and inhibited expressions of pro-inflammatory cytokines in the CCK octapeptide-induced AP. Furthermore, PS pretreatment increased the pancreatic levels of HSP60 and HSP72.


CONCLUSIONS
Pretreatment with PS has an anti-inflammatory effect on CCK octapeptide-induced AP.
Seo
Sang-Wan
SW
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea.
Park
Cheung-Seog
CS
Hong
Seung-Heon
SH
Kwon
Kang-Beom
KB
Moon
Hyoung-Chul
HC
Song
Bong-Keun
BK
Kim
Kyung-Yo
KY
Park
Young-Min
YM
Song
Ho-Joon
HJ
Kim
Hyung-Min
HM
Park
Sung-Joo
SJ
D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D008049:Lipase / Q000097:blood; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D032405:Patrinia / Q000737:chemistry*; D008517:Phytotherapy; D028321:Plant Preparations / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012844:Sincalide
D018834:Chaperonin 60; D016207:Cytokines; D050884:HSP72 Heat-Shock Proteins; D028321:Plant Preparations; D008049:Lipase; D000681:Amylases; D012844:Sincalide