Molecular systematics of Zopfiella and allied genera: evidence from multi-gene sequence analyses.
This study aims to reveal the phylogenetic relationships of Zopfiella and allied genera in the Sordariales. Multiple gene sequences (partial 28S rDNA, ITS/5.8S rDNA and partial beta-tubulin) were analysed using MP and Bayesian analyses. Analyses of different gene datasets were performed individually and then combined to infer phylogenies. Phylogenetic analyses show that currently recognised Zopfiella species are polyphyletic. Based on sequence analyses and morphology, it appears that Zopfiella should be restricted to species having ascospores with a septum in the dark cell. Our molecular analysis also shows that Zopfiella should be placed in Lasiosphaeriaceae rather than Chaetomiaceae. Cercophora and Podospora are also polyphyletic, which is in agreement with previous studies. Our analyses show that species possessing a Cladorrhinum anamorph are phylogenetically closely related. In addition, there are several strongly supported clades, characterised by species possessing divergent morphological characters. It is difficult to predict which characters are phylogenetically informative for delimiting these clades.
Centre for Research in Fungal Diversity, Department of Ecology and Biodiversity, The University of Hong Kong, Pokfulam Rd, Hong Kong SAR, PR China. leicai@hkusua.hku.hk
Lei
L
Cai
Rajesh
R
Jeewon
Kevin D
KD
Hyde
D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012339:RNA, Ribosomal, 28S / Q000737:chemistry / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020037:Sordariales / Q000235:genetics*; D014404:Tubulin / Q000737:chemistry / Q000235:genetics
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S; D014404:Tubulin