In vitro effects of arecoline on sperm motility and cyclooxygenase-2 expression.
Semen samples were obtained from 30 volunteers who had never consumed betel quid. Swim-up spermatozoa from the 30 seminal samples of non-betel quid chewers and also non-smokers, usually not exposed to passive smoking, were treated in vitro with arecoline at different concentrations to evaluate the action of these drugs on sperm motility. Highly motile sperms were collected and divided into 5 equal fractions. Four fractions were supplemented with various concentrations of arecoline and one as control. The study was carried out at time 0 and +1, +2, +3 and +4 hr of incubation. Sperm cells were also extracted and blotted with COX-2 antibody after arecoline treatment after 4 hr incubation. The sperm motility parameters, i.e., motility, average path velocity, curvilinear velocity, straight-line velocity and linearity, were significantly decreased after arecoline treatment. In vitro, arecoline induces the COX-2 expression of sperm cells in a dose-dependent manner. This is the first report to demonstrate that arecoline may mediate COX-2 expression in human sperms, resulting in inflammation response. This situation may act on the structure responsible for the flagellar motion and cause the reduction of sperm motility.
Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
Tze-Kiong
TK
Er
Eing-Mei
EM
Tsai
Li-Yu
LY
Tsai
Ying-Chin
YC
Ko
Jau-Nan
JN
Lee
D000328:Adult; D001114:Areca / Q000737:chemistry; D001115:Arecoline / Q000633:toxicity*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000201:enzymology / Q000502:physiology
D001115:Arecoline; D051546:Cyclooxygenase 2