Urinary liver-type fatty acid binding protein as a useful biomarker in chronic kidney disease.
BACKGROUND
We reported that urinary L-FABP reflected the progression of chronic kidney disease (CKD). This study is aimed to evaluate the clinical significance of urinary liver type fatty acid binding protein (L-FABP) as a biomarker for monitoring CKD.


METHODS
Urinary L-FABP was measured using human L-FABP ELISA kit (CMIC.Co., Ltd., Tokyo, Japan). The relations between urinary L-FABP and clinical parameters were evaluated in non-diabetic CKD (n = 48) for a year. In order to evaluate the influence of serum L-FABP derived from liver upon urinary L-FABP, both serum and urinary L-FABP were simultaneously measured in patients with CKD (n = 73).


RESULTS
For monitoring CKD, the cut-off value in urinary L-FABP was determined as 17.4 microg/g.cr. by using a receiver operating characteristics (ROC) curve. Renal function deteriorated significantly more in patients with 'high' urinary L-FABP (n = 36) than in those with 'low' L-FABP (n = 12). The decrease in creatinine clearance was accompanied by an increase in urinary L-FABP, but not in urinary protein. Serum L-FABP in patients with CKD was not correlated with urinary L-FABP.


CONCLUSIONS
Urinary excretion of L-FABP increases with the deterioration of renal function. Serum L-FABP did not influence on urinary L-FABP. Urinary L-FABP may be a useful clinical biomarker for monitoring CKD.
Internal Medicine, St. Marianna University School of Medicine, Kanagawa, Japan.
Atsuko
A
Kamijo
Takeshi
T
Sugaya
Akihisa
A
Hikawa
Masaya
M
Yamanouchi
Yasunobu
Y
Hirata
Toshihiko
T
Ishimitsu
Atsushi
A
Numabe
Masao
M
Takagi
Hiroshi
H
Hayakawa
Fumiko
F
Tabei
Tokuichiro
T
Sugimoto
Naofumi
N
Mise
Masao
M
Omata
Kenjiro
K
Kimura
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D050556:Fatty Acid-Binding Proteins / Q000097:blood* / Q000652:urine*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000175:diagnosis*; D008099:Liver / Q000378:metabolism; D008297:Male; D008875:Middle Aged
D015415:Biomarkers; C493319:FABP1 protein, human; D050556:Fatty Acid-Binding Proteins