Chain length effects of oligo(L-lysine)-shelled dendrimers on interaction with DNA.
The interaction of novel oligo(L-lysine)-shelled dendrimers (G3-PLL) with DNA was studied by means of circular dichroism spectroscopy, dynamic light scattering, and melting behavior of double-stranded DNA. G3-PLLs having various oligo(L-lysine) (PLL) segment (n = 5-40) were successfully synthesized by graft-polymerization of L-lysine NCA initiated with amino groups at the 3rd-generation poly(amidoamine) dendrimer surface. The ionization property of the newly prepared G3-PLLs were first examined. The evaluated pK(a) values (8.3-8.5) for G3-PLLs were found to be significantly lower than those (9.2-9.4) for the corresponding linear PLLs, probably due to alignment of PLL segments on the three-dimensional core surface. The binding experiments of G3-PLLs to DNA showed that double-stranded DNAs were fairly strongly bound to G3-PLLs primarily through electrostatic interactions. In addition, G3-PLLs served as a DNA cross-linker. A longer PLL-containing G3-PLL was found to interact with DNA more effectively than a shorter one.
Department of Molecular Science and Technology, Faculty of Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan. nhigashi@mail.doshisha.ac.jp
Nobuyuki
N
Higashi
Akiko
A
Uchino
Yuta
Y
Mizuguchi
Masazo
M
Niwa
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D050091:Dendrimers / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008958:Models, Molecular; D009691:Nucleic Acid Denaturation; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D011107:Polylysine / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012995:Solubility; D013696:Temperature; D014867:Water
D003432:Cross-Linking Reagents; D050091:Dendrimers; D009842:Oligopeptides; D014867:Water; D011107:Polylysine; D004247:DNA