Changes in chymotrypsin hydrolysis of beta-lactoglobulin A induced by high hydrostatic pressure.
Beta-lactoglobulin (beta-Lg) was subjected to high pressures up to 400 MPa and proteolysis with chymotrypsin. The hydrolysates were analyzed by SDS-PAGE and RP-HPLC, and the fragments obtained were identified by ESI-MS/MS. The results obtained showed that beta-Lg was hydrolyzed by chymotrypsin in a "progressive proteolysis" manner at either atmospheric or high pressure. Proteolysis during or after high-pressure treatment showed longer and more hydrophobic peptides than proteolysis at atmospheric pressure. Chymotrypsin showed a behavior similar to that of trypsin, with some differences, probably related to the orientation of the target residues specific for each enzyme. The similarities between proteolytic fragments produced by the two enzymes support that proteolysis enhancement under high pressure depends on the substrate changes rather than the enzyme. High pressure seemed to accelerate the first steps of proteolysis, probably through dimer dissociation, while leaving portions of the molecule more resistant to the enzyme.
Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
Rosa
R
Chicón
Rosina
R
López-Fandiño
Ana
A
Quirós
Josefina
J
Belloque
D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000378:metabolism*; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D006874:Hydrostatic Pressure; D007782:Lactoglobulins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D010446:Peptide Fragments / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D013379:Substrate Specificity
D007782:Lactoglobulins; D010446:Peptide Fragments; D002918:Chymotrypsin