Identification of genes for mycothiol biosynthesis in Streptomyces coelicolor A3(2).
Mycothiol is a low molecular weight thiol compound produced by a number of actinomycetes, and has been suggested to serve both anti-oxidative and detoxifying roles. To investigate the metabolism and the role of mycothiol in Streptomyces coelicolor, the biosynthetic genes (mshA, B, C, and D) were predicted based on sequence homology with the mycobacterial genes and confirmed experimentally. Disruption of the mshA, C, and D genes by PCR targeting mutagenesis resulted in no synthesis of mycothiol, whereas the mshB mutation reduced its level to about 10% of the wild type. The results indicate that the mshA, C, and D genes encode non-redundant biosynthetic enzymes, whereas the enzymatic activity of MshB (acetylase) is shared by at least one other gene product, most likely the mca gene product (amidase).
Park
Joo-Hong
JH
School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
Cha
Chang-Jun
CJ
Roe
Jung-Hye
JH
D000115:Acetylesterase / Q000235:genetics / Q000378:metabolism; D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media; D003545:Cysteine; D004187:Disaccharides / Q000096:biosynthesis* / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial; D006020:Glycopeptides; D048088:Informatics; D007294:Inositol; D009154:Mutation; D011720:Pyrazoles / Q000737:chemistry; D048372:Streptomyces coelicolor / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D001426:Bacterial Proteins; D003470:Culture Media; D004187:Disaccharides; D006020:Glycopeptides; D011720:Pyrazoles; D013438:Sulfhydryl Compounds; C089265:mycothiol; D007294:Inositol; D000123:Acetyltransferases; C470312:acetyl-CoA 1-D-myo-inosityl 2-L-cysteinylamido-2-deoxy-alpha-D-glucopyranoside acetyltransferase; D000115:Acetylesterase; D003545:Cysteine