A role for transmembrane domains V and VI in ligand binding and maturation of the angiotensin II AT1 receptor.
Several studies have proposed that angiotensin II (Ang II) binds to its receptor AT1 through interactions with residues in helices V and VI, suggesting that the distance between these helices is crucial for ligand binding. Based on a 3D model of AT1 in which the C-terminus of Ang II is docked, we identified the hydrophobic residues of TM V and VI pointing towards the external face of the helices, which may play a role in the structure of the binding pocket and in the structural integrity of the receptor. We performed a systematic mutagenesis study of these residues and examined the binding, localization, maturation, and dimerization of the mutated receptors. We found that mutations of hydrophobic residues to alanine in helix V do not alter binding, whereas mutations to glutamate lead to loss of binding without a loss in cell surface expression, suggesting that the external face of helix V may not directly participate in binding, but may rather contribute to the structure of the binding pocket. In contrast, mutations of hydrophobic residues to glutamate in helix VI lead to a loss in cell surface expression, suggesting that the external surface of helix VI plays a structural role and ensures correct folding of the receptor.
Pignatari
Graciela C
GC
Department of Biophysics, Escola Paulista de Medicina, UNIFESP, São Paulo 04023-062, Brazil.
Rozenfeld
Raphael
R
Ferro
Emer S
ES
Oliveira
Laerte
L
Paiva
Antonio C M
AC
Devi
Lakshmi A
LA
D000409:Alanine / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D018698:Glutamic Acid / Q000737:chemistry / Q000235:genetics; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D044140:Receptor, Angiotensin, Type 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D008024:Ligands; D008565:Membrane Proteins; D044140:Receptor, Angiotensin, Type 1; D000804:Angiotensin II; D018698:Glutamic Acid; D000409:Alanine