[Effects of different levels of vitamin C on UV radiation-induced DNA damage].
The Raman spectra of DNA in different levels of vitamin C with 10 min ultraviolet radiation and 30 min ultraviolet radiation were reported. The intensity of ultraviolet radiation was 18.68 W x m(-2). The experimental results proved that vitamin C can reduce DNA damage solely, but the effects are related to the concentration of vitamin C. When the concentration of vitamin C was lower than 0.35 mmol x L(-1), DNA was protected better with the rising of vitamin C concentration during ultraviolet radiation. DNA was protected best when vitamin C was 0.35 mmol x L(-1). When vitamin C concentration was higher than 0.35 mmol x L(-1), vitamin C accelerated DNA damage with 10 min ultraviolet radiation. But DNA was also protected with 30 min ultraviolet radiation as vitamin C was 0.35-0.65 mmol x L(-1). However, DNA was only protected slightly at high concentration of vitamin C.
Zhou
Dian-Feng
DF
Jiangsu Provincial Key Lab for Photoelectric Technology, Analysis and Test Center, Nanjing Normal University, Nanjing 210097, China.
Ke
Wei-Zhong
WZ
Chen
Nian-Feng
NF
Ji
Kang
K
D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry*; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D013059:Spectrum Analysis, Raman; D014466:Ultraviolet Rays
D004247:DNA; C427203:calf thymus DNA; D001205:Ascorbic Acid