[The effects of EPHPH on acoustic brainstem response threshold and its relative factors of senile model rats].
OBJECTIVE
The effect of EPHPH on aged deafness and its mechanism was explored by observing the effects of EPHPH on acoustic brainstem response threshold (ABRT), malondialdehyde (MDA), superoxide dismutase (SOD), total-nitrogen monoxide (NOS) and typing-nitrogen monoxide (iNOS) in cochleae tissues of senile model rats.


METHODS
ABRT value was measured by induced potentiometer, MDA was measured by TBA colorimetry, SOD was determined by nitrite method, NOS and iNOS was determined by spectrophotometry in cochleae tissues of senile model rats that separately administered by gavage the alcoholic extract from 2.16, 4.32 or 8.64 g x kg(-1) x d(-1) PHPH once a day for 6 months.


RESULTS
ABRT value and MDA content notably increased, activities) of SOD, NOS and iNOS notably decreased in cochlear tissues of senile model rats from control. It indicated on relative analysis that relativity between ABRT value ascent and MDA ascent showed positive, relaxivities between ABRT value ascent and activity descents of SOD, NOS and iNOS showed all negative. It differently resisted ABRT value ascent and MDA ascent in cochlear tissues that senile model rats separately administered the alcoholic extract from 2.16, 4.32 or 8.64 g x kg(-1) x d(-1) PHPH whose relaxivities between quantity and efficiency showed all negative. It differently inhibited activity descents of SOD, NOS and iNOS in cochlear tissues that senile model rats separately administered various doses of EPHPH whose relaxivities between quantity and efficiency showed all positive.


CONCLUSIONS
EPHPH may relieve the degree of aged deafness and its mechanism may be connected with eliminating oxygen free radicals and increasing the activities of SOD, NOS and iNOS in cochleae of natural senile rats.
Yuan
Yiming
Y
Department of Otolaryngology, Second Affiliated Hospital of Lanzhou Medical College, Lanzhou 730030, China.
Ge
Bin
B
Xu
Aixia
A
Gao
Xiang
X
Zhang
Zhenming
Z
D000375:Aging / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D001309:Auditory Threshold; D016057:Evoked Potentials, Auditory, Brain Stem; D005260:Female; D008315:Malondialdehyde / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D010936:Plant Extracts; D008315:Malondialdehyde; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase