Diagnostic surface expression of SWAP-70 on HIV-1 infected T cells.
Following immunization with HIV-1 infected cells, a hybridoma cell line termed 9F11 was established from the P3U-1 myeloma line fused with lymphocytes from a trans-chromosome (TC) mouse, that harbors human chromosomes containing immunoglobulin genes. The 9F11 human IgM monoclonal antibody (9F11 Ab) reacts with HIV-1 infected MOLT4 cells but not with uninfected MOLT4 cells, and causes immune cytolysis with homologous human complement at a concentration as low as 0.4 microg/ml. This Ab was used to perform immunoscreening of a cDNA expression library derived from HIV-1 infected cells. All positive cDNA clones contained SWAP-70 cDNA. SWAP-70 RNA and protein expression are much stronger in HIV-1 infected cells. SWAP-70 was also detected on the surface of HIV-1 infected cells by flow cytometric analysis. The monocyte cell line U937 cells expresses SWAP-70 on its cell surface regardless of whether it was infected with HIV-1. Furthermore, among PBMCs surface expression of SWAP-70 was detected on CD21+, CD56+ and CD14+ cells. Although CD3+ cells scarcely express SWAP-70 on their surface, once activated, they become positive. SWAP-70 may therefore serve as a marker for T cell differentiation as well as for HIV-1 infection.
Kimbara
Noriaki
N
Department of Biodefense Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467-8601, Japan.
Dohi
Natsuki
N
Miyamoto
Mizuyu
M
Asai
Suzuka
S
Okada
Hidechika
H
Okada
Noriko
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002454:Cell Differentiation; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D007075:Immunoglobulin M / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000821:virology; D008213:Lymphocyte Activation; D051379:Mice; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000821:virology*; D020298:U937 Cells
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; D007075:Immunoglobulin M; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins; C491873:SWAP70 protein, human