Cell-permeable and biocompatible polymeric nanoparticles for apoptosis imaging.
Here, we report for the first time cell-permeable and biocompatible polymeric nanoparticles consisting of a polymer conjugated to a near-infrared (NIR) fluorescence (Cy5.5)-linked effector caspase-specific peptide. The close spatial proximity of the NIR fluorochromes in polymeric nanoparticles results in an autoquenched state, but polymer nanoparticles give rise to strong NIR fluorescence signal under apoptotic cells. Thus, the smart polymeric nanoparticle developed here is an attractive probe for real-time imaging of apoptosis in single cells.
Biomedical Research Center, Korea Institute of Science and Technology, 39-1 Haweolgog-Dong, Sungbook-Gu, Seoul 136-791, Korea.
Kwangmeyung
K
Kim
Misu
M
Lee
Hyungkyu
H
Park
Jong-Ho
JH
Kim
Sungwon
S
Kim
Hesson
H
Chung
Kuiwon
K
Choi
In-San
IS
Kim
Baik L
BL
Seong
Ick Chan
IC
Kwon
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics*; D002232:Carbocyanines / Q000737:chemistry; D053148:Caspase 3; D053179:Caspase 7; D020169:Caspases / Q000378:metabolism; D002463:Cell Membrane Permeability; D003840:Deoxycholic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008562:Membrane Glycoproteins / Q000494:pharmacology; D049329:Nanostructures / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D011094:Polyethyleneimine / Q000737:chemistry / Q000493:pharmacokinetics; D012542:Scattering, Radiation; D019265:Spectroscopy, Near-Infrared; D013379:Substrate Specificity; D053221:TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D051017:Apoptosis Regulatory Proteins; D001672:Biocompatible Materials; C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D008562:Membrane Glycoproteins; D009842:Oligopeptides; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D014409:Tumor Necrosis Factor-alpha; D003840:Deoxycholic Acid; D011094:Polyethyleneimine; C505407:CASP3 protein, human; C505520:CASP7 protein, human; D053148:Caspase 3; D053179:Caspase 7; D020169:Caspases