Contour and volume assessment of repairing mandibular osteoperiosteal continuity defects in sheep using recombinant human osteogenic protein 1.
BACKGROUND
This study describes the contour and volume of reconstructed mandibles using recombinant human osteogenic protein 1.


METHODS
The investigation was conducted on six adult sheep, where a unilateral 35 mm parasymphyseal osteoperiosteal continuity defect of the mandible was created. Recombinant human osteogenic protein 1 and type-I collagen (as carrier) were applied to the defects. Radiographic and ultrasonographic examinations were carried out at day 1 of the surgery and 2, 4, 8, and 12 weeks following the surgery. The animals were then sacrificed 3 months after the operation. Postmortem CT-scan was performed for volumetric, cross-sectional area, height and width measurements.


RESULTS
Ultrasound was more efficient than radiographs in demonstrating early callus formation at 2 weeks, while radiographic evidence of bone formation was consistently detectable only after 4 weeks. Using the combination of recombinant human osteogenic protein type 1 and type-I collagen resulted in twice the volume, cross-sectional surface area, and height when compared with those of the corresponding region of the contra-lateral non-operated side of the mandible.


CONCLUSIONS
Within 3 months, recombinant human osteogenic protein type 1 on type-I collagen carrier failed to restore the original contour and volume of mandibular osteoperiosteal continuity defects.
Biotechnology and Craniofacial Research Group, Glasgow Dental Hospital & School and the West of Scotland Oral & Maxillofacial Surgery Service, Glasgow, UK. ml1711990@hotmail.com
Muammar
M
Abu-Serriah
Ashraf
A
Ayoub
David
D
Wray
Nicola
N
Milne
Stuart
S
Carmichael
Jack
J
Boyd
D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000627:therapeutic use*; D002146:Bony Callus / Q000502:physiology; D002417:Cattle; D024042:Collagen Type I / Q000008:administration & dosage; D005260:Female; D006801:Humans; D008334:Mandible / Q000000981:diagnostic imaging / Q000601:surgery*; D010012:Osteogenesis / Q000502:physiology*; D011994:Recombinant Proteins / Q000627:therapeutic use; D015203:Reproducibility of Results; D012756:Sheep; D014057:Tomography, X-Ray Computed; D014463:Ultrasonography
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I; D011994:Recombinant Proteins