Three-dimensional nanohydroxyapatite/chitosan scaffolds as potential tissue engineered periodontal tissue.
The development of suitable three-dimensional scaffold for the maintenance of cellular viability and differentiation is critical for applications in periodontal tissue engineering. In this work, different ratios of porous nanohydroxyapatite/chitosan (HA/chitosan) scaffolds are prepared through a freeze-drying process. These scaffolds are evaluated in vitro by the analysis of microscopic structure, porosity, and cytocompatibility. The expression of type I collagen and alkaline phosphatase (ALP) activity are detected with real-time polymerase chain reaction (RT-PCR). Human periodontal ligament cells (HPLCs) transfected with enhanced green fluorescence protein (EGFP) are seeded onto the scaffolds, and then these scaffolds are implanted subcutaneously into athymic mice. The results indicated that the porosity and pore diameter of the HA/chitosan scaffolds are lower than those of pure chitosan scaffold. The HA/chitosan scaffold containing 1% HA exhibited better cytocompatibility than the pure chitosan scaffold. The expression of type I collagen and ALP are up-regulated in 1% HA/chitosan scaffold. After implanted in vivo, EGFP-transfected HPLCs not only proliferate but also recruit surrounding tissue to grow in the scaffold. The degradation of the scaffold significantly decreased in the presence of HA. This study demonstrated the potential of HA/ chitosan scaffold as a good substrate candidate in periodontal tissue engineering.
Zhang
Yu-Feng
YF
Ministry Education Key Laboratory for Oral Biomedical Engineering School of Stomatology, Wuhan University, Wuhan 430079, PR China.
Cheng
Xiang-Rong
XR
Chen
Yun
Y
Shi
Bin
B
Chen
Xiao-Hui
XH
Xu
Dong-Xuan
DX
Ke
Jin
J
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D003764:Dental Materials / Q000737:chemistry / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016062:Porosity; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering
D001672:Biocompatible Materials; D024042:Collagen Type I; D003764:Dental Materials; D012333:RNA, Messenger; D048271:Chitosan; D017886:Durapatite; D000469:Alkaline Phosphatase