[Protective effects of total alkaloids from rhizoma Coptis chinensis on alcohol-induced gastric lesion in rats].
OBJECTIVE
To study the effects of total alkaloids(TA) from rhizoma Coptis chinensis on alcohol-induced gastric lesion in rats and the possible mechanisms.


METHODS
The experimental gastric damges were established by intragastric(ig) absolute ethanol, and possible protective effects of TA given orally previously were evaluated by following parameters: gastric damage indexes, gastric juice volume, acidity, and mucus quantity. The contents of NO, MDA, *OH, and SOD activity were also measured in gastric mucosa.


RESULTS
TA showed significantly inhibitive effects on gastric damages induced by ig ethanol in a dose dependent manner. The effects of TA (120 mg x kg(-1)) were stronger than that of both cimitidine(70 mg x kg(-1)) and berberine(100 mg x kg(-1)), the quantity of later was equal to TA as calculated with berberine. TA significantly suppressed secretion of gastric acid caused by ethanol without clear influences on gastric juice volume and mucus secretion. TA obviously blunted ethanol-induced elevation of MDA and *OH, as well as decrease of NO level and SOD activity from gastric mucosa.


CONCLUSIONS
It is suggested that the TA is a potent protective agent against ethanol-induced gastric damages. The mechanism of actions may be related with inhibiting the secretion of gastric acid and blunting the increase of MDA and *OH, as well as the decrease of NO level and SOD activity from gastric mucus.
Chongqing University of Medical Sciences, China.
Bei
B
Li
Hua-rong
HR
Liu
Yong-quan
YQ
Pan
Qing-song
QS
Jiang
Jing-chuan
JC
Shang
Xian-hui
XH
Wan
Bai-cheng
BC
He
Jun-qing
JQ
Yang
Qi-xin
QX
Zhou
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D039323:Coptis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D027343:Rhizome / Q000737:chemistry; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D000431:Ethanol