Antibacterial effect of selected root-end filling materials.
The purpose of this study was to evaluate the antibacterial activity of leachable components of selected root-end filling materials: amalgam, ProRoot MTA (mineral trioxide aggregate), Intermediate Restorative Material (IRM), Super Bond C&B, Geristore, Dyract, Clearfil APX composite with SE Bond, or Protect Bond. The direct contact test (DCT) with Staphylococcus aureus, Enterococcus faecalis, and Pseudomonas aeruginosa, was used. The materials were tested immediately after application to the microtiter wells (fresh samples) and after setting for 3 days (set samples). Ten microliters of bacterial suspension was added to each well for direct contact with each material for 1 h at 37 degrees C. Growth of surviving bacteria was then measured in a microplate spectrophotometer hourly at 620 nm for 15 h. Twelve uncoated wells using identical inoculum size served as positive controls. The data obtained at the end of 15 h was subjected to one-way ANOVA and post hoc comparisons were done using Tamhane's T2 test. Fresh samples of all materials showed a 3-h delay in exponential growth of both E. faecalis and S. aureus, and a 5-h delay in growth of P. aeruginosa. Set samples of IRM and ProRoot MTA cements showed generally greater antibacterial activity than the other materials: both completely inhibited P. aeruginosa, and both delayed or limited growth of E. faecalis. The DCT, by being quantitative and virtually independent of solubility and diffusion, was found suitable to assay solid root-end filling materials. IRM and ProRoot MTA were generally more potent inhibitors of bacterial-growth than the other tested materials.
Eldeniz
Ayce Unverdi
AU
Department of Endodontics, Faculty of Dentistry, University of Selcuk, Konya, Turkey. ayce71@hotmail.com
Hadimli
Hasan Huseyin
HH
Ataoglu
Hanife
H
Orstavik
Dag
D
D017607:Aluminum Compounds / Q000494:pharmacology; D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001896:Boron Compounds / Q000494:pharmacology; D017610:Calcium Compounds / Q000494:pharmacology; D020770:Compomers / Q000494:pharmacology; D003723:Dental Amalgam / Q000494:pharmacology; D017220:Dentin-Bonding Agents / Q000494:pharmacology; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects; D005899:Glass Ionomer Cements / Q000494:pharmacology; D008689:Methacrylates / Q000494:pharmacology; D008768:Methylmethacrylates / Q000494:pharmacology; D050296:Microbial Viability / Q000187:drug effects; D010087:Oxides / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D019279:Resin Cements / Q000494:pharmacology; D012117:Resins, Synthetic / Q000494:pharmacology; D012184:Retrograde Obturation / Q000379:methods; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
D017607:Aluminum Compounds; D000891:Anti-Infective Agents, Local; D001896:Boron Compounds; D017610:Calcium Compounds; C503075:Clearfil Protect Bond; C438313:Clearfil SE Bond; D020770:Compomers; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; C088486:Dyract; C074933:Geristore; D005899:Glass Ionomer Cements; D008689:Methacrylates; D008768:Methylmethacrylates; D010087:Oxides; D019279:Resin Cements; D012117:Resins, Synthetic; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C086631:mineral trioxide aggregate; C031667:IRM cement; D003723:Dental Amalgam; C049495:Super-bond