Tissue engineering of small intestinal tissue using collagen sponge scaffolds seeded with smooth muscle cells.
In a previously reported attempt to regenerate small intestine with autologous tissues, collagen scaffolds were used without cell seeding or with autologous mesenchymal stem cell seeding. However the regenerated intestine lacked a smooth muscle layer. To accomplish regeneration of a smooth muscle layer, this present study used collagen scaffolds seeded with the smooth muscle cells (SMC) in a canine model. Autologous SMC were isolated from stomach wall and cultured. Two types of scaffolds were fabricated: in SMC (+), cultured SMCs were mixed with collagen solution and poured into a collagen sponge; and in SMC (-), SMCs were omitted. Both scaffolds were implanted into defects of isolated ileum as a patch graft. Animals were euthanized at 4, 8, and 12 weeks; for the last time point, the ileal loop had been reanastomosed at 8 weeks. At 12 weeks, the SMC (-) group showed a luminal surface covered by a regenerated epithelial cell layer with very short villi; however only a thin smooth muscle layer was observed, representing the muscularis mucosae. In the SMC (+) group, the luminal surface was covered completely by a relatively well-developed epithelial layer with numerous villi. Implanted SMCs were seen in the lamina propria and formed a smooth muscle layer. Thus, we concluded that collagen sponge scaffolds seeded with autologous SMCs have a potential for small intestine regeneration.
Nakase
Yuen
Y
Department of Surgery and Regenerative Medicine, Division of Surgery and Physiology of Digestive System, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan. yuen-n@koto.kpu-m.ac.jp
Hagiwara
Akeo
A
Nakamura
Tatsuo
T
Kin
Syuichi
S
Nakashima
Susumu
S
Yoshikawa
Tetsuji
T
Fukuda
Ken-Ichirou
K
Kuriu
Yoshiaki
Y
Miyagawa
Kouji
K
Sakakura
Chohei
C
Otsuji
Eigo
E
Shimizu
Yasuhiko
Y
Ikada
Yoshihito
Y
Yamagishi
Hisakazu
H
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry*; D004285:Dogs; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D020007:Implants, Experimental; D007421:Intestine, Small / Q000166:cytology*; D045164:Mesenchymal Stem Cell Transplantation; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000166:cytology* / Q000648:ultrastructure; D013270:Stomach; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate