Interferon-beta antibodies have a higher affinity in patients with neutralizing antibodies compared to patients with non-neutralizing antibodies.
In this study, we investigated the affinity, determined by a relative affinity assay, using increasing concentrations of sodium-isothiocyanate to disrupt the antigen antibody binding of neutralizing and non-neutralizing antibodies against interferon-beta (IFNbeta)-1a and -1b in 73 serum samples of MS patients treated with IFNbeta-1a or -1b. Relative affinity values were significantly higher in NAB-positive compared to NAB-negative samples and in samples of IFNbeta-1a-treated patients compared to IFNbeta-1b. A significant positive correlation between relative affinity values and therapy duration indicates affinity maturation as another qualitative factor in IFNbeta neutralization.
Department of Neurology, Innsbruck Medical University, Anichstrasse 35, 6020 Innsbruck, Austria.
C
C
Gneiss
P
P
Tripp
R
R
Ehling
M
M
Khalil
A
A
Lutterotti
R
R
Egg
I
I
Mayringer
B
B
Künz
T
T
Berger
M
M
Reindl
F
F
Deisenhammer
D000704:Analysis of Variance; D000906:Antibodies / Q000502:physiology / Q000627:therapeutic use*; D001667:Binding, Competitive / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D016899:Interferon-beta / Q000276:immunology* / Q000378:metabolism; D009103:Multiple Sclerosis / Q000276:immunology / Q000628:therapy*; D009500:Neutralization Tests / Q000379:methods; D013997:Time Factors; D016896:Treatment Outcome
D000906:Antibodies; D016899:Interferon-beta