Optimization of medium and cultivation conditions for alkaline protease production by the marine yeast Aureobasidium pullulans.
A yeast strain, Aureobasidium pullulans, which could produce the high yield of protease was isolated from sediment of saltern in Qingdao, China. Maximum production of enzyme (623.1 U/mg protein; 7.2 U/ml) was obtained in a medium containing 2.5 g soluble starch and 2.0 g NaNO(3), 100ml seawater, initial pH 6.0, after fermentation at 24.5 degrees C for 30 h. The protease had the highest activity at pH 9.0 and 45 degrees C.
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UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China. zhenming@sdu.edu.cn
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Wang
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Li
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D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development; D001426:Bacterial Proteins / Q000378:metabolism*; D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D010450:Endopeptidases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
D001426:Bacterial Proteins; D003470:Culture Media; D002244:Carbon; D010450:Endopeptidases; C087822:alkaline protease; D009584:Nitrogen