Metabolic support of moderate activity differs from patterns seen after extreme behavior in the desert iguana Dipsosaurus dorsalis.
This study examined glucose and lactate metabolism in an iguanid lizard, Dipsosaurus dorsalis, during rest and after activity patterned on field behavior (15 s of running at 1 m/s). Metabolite oxidation and incorporation into glycogen by the whole animal, the liver, and oxidative and glycolytic muscle fibers were measured using (14)C- and (13)C-labeled compounds. Results showed that lactate metabolism is more responsive to changes that occurred between rest and recovery, whereas glucose appears to play a more steady state role. After activity, lactate oxidation produced 57 times as much ATP during 1 h of recovery than did glucose oxidation. However, lactate oxidation rates were elevated for only 30 min after activity, while glucose oxidation remained elevated beyond 1 h. Lactate was the primary source for glycogen synthesis during recovery, and glucose was the main glycogenic substrate during rest. This study supports previous research showing that brief activity in D. dorsalis is primarily supported by glycolysis and phosphocreatine breakdown, but it also suggests that there may be less of a reliance on glycolysis and a greater reliance on phosphocreatine than previously shown. The findings presented here indicate that the metabolic consequences of the behaviorally relevant activity studied are less severe than has been suggested by studies using more extreme activity patterns.
Department of Integrative Physiology, University of Colorado, Boulder, CO 80309-0354, USA. donovane@colorado.edu
Edward R
ER
Donovan
Todd T
TT
Gleeson
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D003889:Desert Climate; D005260:Female; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000097:blood / Q000378:metabolism; D006019:Glycolysis / Q000502:physiology; D007076:Iguanas / Q000378:metabolism*; D019344:Lactic Acid / Q000097:blood / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009043:Motor Activity / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism
D019344:Lactic Acid; D000255:Adenosine Triphosphate; D006003:Glycogen; D005947:Glucose