Effects of blockade of cerebral lymphatic drainage on regional cerebral blood flow and brain edema after subarachnoid hemorrhage.
The study was designed to observe the influence of blockade of cerebral lymphatic drainage on the regional cerebral blood flow (rCBF) and brain edema after experimental subarachnoid hemorrhage (SAH). Wistar rats were divided into non-SAH, SAH, and SAH plus cervical lymphatic blockade (SAH + CLB) groups. Autologous arterial hemolysate was injected into rat's cisterna magna to induce SAH. The rCBF was recorded continuously by a laser Doppler flowmeter. Intracranial pressure (ICP) was also monitored. After 24 hours and 72 hours of SAH, the rats were sacrificed and the brain was harvested for water content detection. It was found that there was no obvious change of rCBF and brain water content during the experiment in non-SAH group. An immediate and persistent drop in rCBF was found in SAH group. The drop in rCBF was more obvious in SAH + CLB group. CLB also worsened the SAH-induced increase in ICP. The brain water content 24 hours and 72 hours after induction of SAH in SAH group increased significantly. CLB led to a further increase of brain water content. In conclusion, blockade of cerebral lymphatic drainage pathway deteriorates the secondary cerebral ischemia and brain edema after SAH.
Sun
Bao-Liang
BL
Institute of Microcirculation & Department of Neurology, Affiliated Hospital, Taishan Medical College, Taian, Shandong 271000, China. blsun@tsmc.edu.cn
Xia
Zuo-Li
ZL
Wang
Jing-Ru
JR
Yuan
Hui
H
Li
Wen-Xia
WX
Chen
Yu-She
YS
Yang
Ming-Feng
MF
Zhang
Su-Ming
SM
D000818:Animals; D001783:Blood Flow Velocity; D001921:Brain; D001929:Brain Edema / Q000209:etiology*; D002545:Brain Ischemia / Q000209:etiology*; D002560:Cerebrovascular Circulation; D004195:Disease Models, Animal; D042601:Lymphatic Vessels / Q000293:injuries*; D051381:Rats; D017208:Rats, Wistar; D013345:Subarachnoid Hemorrhage / Q000150:complications*; D014867:Water / Q000032:analysis
D014867:Water