Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome.
Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence [mHSFYL (Riken cDNA 4933413G11Rik)], which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA- or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis.
Kinoshita
Keigo
K
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School, Japan.
Shinka
Toshikatsu
T
Sato
Youichi
Y
Kurahashi
Hiroki
H
Kowa
Hiroe
H
Chen
Gang
G
Umeno
Mayumi
M
Toida
Kazunori
K
Kiyokage
Emi
E
Nakano
Takuro
T
Ito
Susumu
S
Nakahori
Yutaka
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D041322:Chromosomes, Human, Y / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009845:Oligospermia / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013087:Spermatids / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors