Characterization of semenogelin proteins in the human retina.
Semenogelin I and II are the major proteins present in semen coagulum. In the present study, semenogelin I and II were detected in human RPE lysates by proteomic analysis. We further analyzed the expression of these proteins in the retinal cells in vivo and in vitro. Western blots detected semenogelin I and II in both RPE and neural retina while the vitreous contained only SgII. Cryo and paraffin sections of human retina were processed for both immunofluorescence and DAB reaction with an antibody that recognizes both forms of semenogelin proteins. Retina and RPE total lysates were evaluated for the presence of these proteins and in a human RPE cell line (D407). Both proteins were detected by western blot in human RPE and in D407 cell lysates. Immunoreactivity was detected in the ganglion cell and photoreceptor layer of the retina. Our data support the expression of semenogelin I and II in the human retina in several different compartments. Further studies towards addressing the function of these proteins in the retina are in progress.
Bonilha
Vera L
VL
Department of Ophthalmic Research, The Cole Eye Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH, 44195, USA. bonilhav@ccf.org
Rayborn
Mary E
ME
Shadrach
Karen
K
Lundwall
Ake
A
Malm
Johan
J
Bhattacharya
Sanjoy K
SK
Crabb
John W
JW
Hollyfield
Joe G
JG
D000368:Aged; D000369:Aged, 80 and over; D002829:Choroid / Q000737:chemistry; D005136:Eye Proteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008875:Middle Aged; D020419:Photoreceptor Cells, Vertebrate / Q000737:chemistry; D010857:Pigment Epithelium of Eye / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D012160:Retina / Q000737:chemistry* / Q000166:cytology; D012165:Retinal Ganglion Cells / Q000737:chemistry; D029607:Seminal Vesicle Secretory Proteins / Q000032:analysis*; D014822:Vitreous Body / Q000737:chemistry
D005136:Eye Proteins; D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen