[Blocking action of pyridoxal-5'-phosphate on nonadrenergic synaptic inhibition of human small intestine smooth muscle].
We investigated the inhibitory synaptic potentials (ISP) in isolated smooth muscle strips of the human duodenum circular layer from the ulcer adjacent region (I group) as well as ileum and distal part of small intestine, which were on a distance of some dozen centimeters from the place of disturbance under the different gastrointestinal diseases (II group). ISP amplitude was several times smaller in the muscle strips of the I group compare to the II group. It could depend on the alterations of smooth muscles cable properties, increase of connective tissue mass, changes in the intestinal nervous system and synaptic transmission in the region adjacent to duodenum ulcer. Pyridoxal-5'-phosphate effectively decreased amplitude and increased ISP latent period in the muscle strips from both groups. N(omega)-nitro-L-arginine, the blocker of NO-synthase did not affect pyridoxal-5'-phosphate activity in smooth muscles. Phosphate group was essential for realization of its influence on ISP in smooth muscles because pyridoxal did not influence both ISP amplitude and ISP latent period.
O V
OV
Romanenko
M M
MM
Grusha
P D
PD
Fomin
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D007410:Intestinal Diseases / Q000378:metabolism / Q000503:physiopathology*; D007421:Intestine, Small / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D008875:Middle Aged; D009130:Muscle, Smooth / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000494:pharmacology; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D009435:Synaptic Transmission / Q000187:drug effects*
D004791:Enzyme Inhibitors; D019335:Nitroarginine; D011732:Pyridoxal Phosphate; D019001:Nitric Oxide Synthase