Expression of Barley Endopeptidase B in Trichoderma reesei.
The gene for barley endopeptidase B (EPB) has been expressed in the filamentous fungus Trichoderma reesei from the cbh1 promoter. The EPB signal sequence allowed secretion of over 90% of the recombinant protein. Yields reached about 500 mg of immunoreactive protein per liter and exceeded values for any other protein derived from a higher eukaryotic organism produced in T. reesei.
R
R
Saarelainen
A
A
Mantyla
H
H
Nevalainen
P
P
Suominen