The vibrissal motor output following severing and repair of the facial nerve in the newborn rat reorganises less than in the adult.
This study examined the ability of facial motoneurons and motor cortex to reorganise their relationship with the somatic musculature following the severing and repair of the facial nerve in rats at birth. In each adult rat, the organisation of the facial nucleus and the cortical motor output corresponding to the normal side were compared with those corresponding to the reinnervated side. Labelling was used to reveal reinnervation-induced long-term changes in the motoneuron pool supplying vibrissal muscles. Cortical motor output was assessed by mapping the vibrissal movement area extension and thresholds evoked by intracortical microstimulation. After facial nerve reinnervation: (i) the proportion of labelled cell profiles decreased by 85.2% of that in the control side and cortical representation of vibrissal movement decreased by 66.3% of that in control hemispheres; (ii) the reorganised vibrissal representation was shrunken to the medialmost portion of the normal vibrissal representation and there was a medial extension of the forelimb representation, and a more modest lateral extension of eye representation, into the vibrissal territory; (iii) the normal pattern of contralateral vibrissal movement was observed in only 10% of the vibrissal sites, whereas ipsilateral vibrissal movement was found in 53% of the vibrissal sites; (iv) there was an increase in the mean threshold required to evoke contralateral vibrissal movement (32.5+/-11.1 vs. 20.5+/-6.9 microA). Thresholds to evoke other types of movement were similar to normal. These changes indicate that an incomplete motor axon regeneration at birth does not restore normal innervation and normal cortical control over the vibrissal muscles.
Franchi
Gianfranco
G
Dipartimento di Scienze Biomediche e Terapie Avanzate, Sezione di Fisiologia umana e Centro di Neuroscienze Università di Ferrara, 44100 Ferrara, Italy. fhg@dns.unife.it
Maggiolini
Emma
E
Muzzioli
Valerio
V
Guandalini
Paola
P
D000200:Action Potentials / Q000502:physiology; D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001931:Brain Mapping; D003627:Data Interpretation, Statistical; D004594:Electrophysiology; D005154:Facial Nerve / Q000502:physiology*; D020220:Facial Nerve Injuries / Q000473:pathology / Q000503:physiopathology; D007839:Functional Laterality / Q000502:physiology; D006735:Horseradish Peroxidase; D009044:Motor Cortex / Q000166:cytology / Q000502:physiology; D009046:Motor Neurons / Q000502:physiology*; D009068:Movement / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014738:Vibrissae / Q000294:innervation / Q000502:physiology*
D006735:Horseradish Peroxidase