Proteomic analysis of serum marker proteins in recipient mice with liver cirrhosis after bone marrow cell transplantation.
We previously found that transplantation with bone marrow cells (BMCs) improves liver function and liver fibrosis in cirrhotic mice. In the presence of liver damage induced by carbon tetrachloride (CCl4), transplanted BMC migrated into the peri-portal region and trans-differentiated into hepatocytes that produce albumin. Thus under these conditions, BMC transplantation induces liver regeneration. Detecting serum marker proteins is important to monitor the recovery of liver function of cirrhotic mice after BMC transplantation. We therefore initially resolved proteins extracted from serum samples at 48 h after BMC transplantation by 2-DE and compared spot intensity between control and BMC groups of mice. Six protein spots increased in the BMC group compared with the control group. MS revealed that these spots comprised apolipoprotein A1 (apoA1), apolipoprotein C3 (apoC3), vitamin D-binding protein, alpha-1-antitrypsin and proteasome subunit alpha type 1. We subsequently confirmed the levels of apoA1 in serum and liver samples by immunoblotting. ApoA1 increased at early stage (48 h and 1 wk) after BMC transplantation in this mouse model of liver cirrhosis. The early elevation of apoA1 might be useful to predict liver regeneration in cirrhotic mice after BMC transplantation.
Yokoyama
Yuichiro
Y
Department of Molecular Science and Applied Medicine (Gastroenterology and Hepatology), Yamaguchi University School of Medicine, Minami Kogushi, Ube, Yamaguchi, Japan.
Terai
Shuji
S
Ishikawa
Tsuyoshi
T
Aoyama
Koji
K
Urata
Yohei
Y
Marumoto
Yoshio
Y
Nishina
Hiroshi
H
Nakamura
Kazuyuki
K
Okita
Kiwamu
K
Sakaida
Isao
I
D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood; D001056:Apolipoproteins C / Q000097:blood; D016026:Bone Marrow Transplantation / Q000009:adverse effects* / Q000379:methods*; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002465:Cell Movement; D008099:Liver / Q000502:physiology; D008103:Liver Cirrhosis / Q000209:etiology*; D051379:Mice; D046988:Proteasome Endopeptidase Complex; D011506:Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012038:Regeneration; D014809:Vitamin D-Binding Protein / Q000097:blood; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis
D016632:Apolipoprotein A-I; D001056:Apolipoproteins C; D011506:Proteins; D014809:Vitamin D-Binding Protein; D000515:alpha 1-Antitrypsin; D002251:Carbon Tetrachloride; D046988:Proteasome Endopeptidase Complex; C486213:Psme1 protein, mouse