Evaluation, in vitro, of the radioprotection of DNA from gamma-rays by naphazoline.
Design of compounds that can protect efficiently against gamma-rays irradiation is a great challenge. An ionizing event can cause variety of DNA damage scenarios leading to mutagenesis, cell death. 2-(1-Naphthylmethyl)-2-imidazoline (naphazoline, NP) is a drug belonging to the vasoregulator class, which was shown to be a very interesting compound in radioprotection. In order to highlight the NP radioprotective activity, a comparison of its ability to protect DNA against either gamma-irradiation or radicals generated by Fenton's reaction was made. Results show that NP inhibits efficiently the generation of DNA single-strand breaks and that NP is a potent radioprotector and also an hydroxyl radical scavenger.
Laboratoire Hétérochimie fondamentale et appliquée, UMR 5069-CNRS/UPS, université Paul-Sabatier, 118, route de Narbonne, 31062 Toulouse cedex 9, France.
Caroline
C
Prouillac
Benoît
B
Célariès
Patricia
P
Vicendo
Ghassoub
G
Rima
D004247:DNA / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D004249:DNA Damage; D004279:DNA, Viral / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D005720:Gamma Rays; D017665:Hydroxyl Radical; D016296:Mutagenesis; D009278:Naphazoline / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D004279:DNA, Viral; D011837:Radiation-Protective Agents; D017665:Hydroxyl Radical; D004247:DNA; D009278:Naphazoline