Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.).
Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypocotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.
Rao
Abdul Qayyum
AQ
Institution Centre of Excellence in Molecular Biology, University of the Punjab, Lahore 53700, Pakistan.
Hussain
S Sarfraz
SS
Shahzad
M Saqib
MS
Bokhari
S Yassir Abbas
SY
Raza
M Hashim
MH
Rakha
Allah
A
Majeed
A
A
Shahid
A Ali
AA
Saleem
Zafar
Z
Husnain
Tayyab
T
Riazuddin
S
S
D015084:2,4-Dichlorophenoxyacetic Acid; D001593:Benzyl Compounds; D018548:Cotyledon / Q000254:growth & development; D003470:Culture Media; D003368:Gossypium / Q000196:embryology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018546:Hypocotyl / Q000254:growth & development; D007701:Kinetin; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D012038:Regeneration / Q000502:physiology*; D014170:Transformation, Genetic; D015026:Zeatin
D001593:Benzyl Compounds; D003470:Culture Media; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; D015026:Zeatin; C480551:benzylaminopurine; D007701:Kinetin