Immobilization of phospholipid-avidin on fused-silica capillaries for chiral separation in open-tubular capillary electrochromatography.
Phospholipid-coated fused-silica capillaries with immobilized avidin were applied in the chiral separation of D,L-tryptophan, D,L-PTH-serine, and D,L-PTH-threonine at pH 7.4 by open-tubular CEC. Liposomes prepared from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(Cap biotinyl), or 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(Biotinyl) with different amounts of phosphatidylserine were assessed as phospholipid coating materials. The stability of the coating and the success of the coating procedure were evaluated in terms of the repeatability of the enantiomer migration times and the resolution of enantiomers. The coating procedure itself significantly affected the migration times and resolution of the enantiomers. Reliable chiral separations with high separation efficiencies were achieved through careful choice of the coating method.
Han
Nan-Yin
NY
Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Helsinki, Finland.
Hautala
Jari T
JT
Bo
Tao
T
Wiedmer
Susanne K
SK
Riekkola
Marja-Liisa
ML
D000327:Adsorption; D001360:Avidin / Q000737:chemistry*; D019921:Biotinylation; D002845:Chromatography / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008081:Liposomes; D010669:Phenylthiohydantoin / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D012694:Serine / Q000031:analogs & derivatives / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism; D013912:Threonine / Q000031:analogs & derivatives / Q000302:isolation & purification; D014364:Tryptophan / Q000302:isolation & purification
D008081:Liposomes; D010743:Phospholipids; D001360:Avidin; D010669:Phenylthiohydantoin; D013912:Threonine; D012694:Serine; D012822:Silicon Dioxide; D014364:Tryptophan