Disentangling information flow in the Ras-cAMP signaling network.
The perturbation of signal-transduction molecules elicits genomic-expression effects that are typically neither restricted to a small set of genes nor uniform. Instead there are broad, varied, and complex changes in expression across the genome. These observations suggest that signal transduction is not mediated by isolated pathways of information flow to distinct groups of genes in the genome. Rather, multiple entangled paths of information flow influence overlapping sets of genes. Using the Ras-cAMP pathway in Saccharomyces cerevisiae as a model system, we perturbed key pathway elements and collected genomic-expression data. Singular value decomposition was applied to separate the genome-wide transcriptional response into weighted expression components exhibited by overlapping groups of genes. Molecular interaction data were integrated to connect gene groups to perturbed signaling elements. The resulting series of linked subnetworks maps multiple putative pathways of information flow through a dense signaling network, and provides a set of testable hypotheses for complex gene-expression effects across the genome.
Institute for Systems Biology, Seattle, Washington 98103, USA. gcarter@systemsbiology.org
Gregory W
GW
Carter
Steffen
S
Rupp
Gerald R
GR
Fink
Timothy
T
Galitski
D000242:Cyclic AMP / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics*; D008957:Models, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D018631:ras Proteins / Q000235:genetics*
D029701:Saccharomyces cerevisiae Proteins; D000242:Cyclic AMP; C489015:RAS2 protein, S cerevisiae; D018631:ras Proteins