Cytoskeleton changes following differentiation of N1E-115 neuroblastoma cell line.
No systematic approach to detect expression of differentiation-related elements was published so far. The undifferentiated N1E-115 neuroblastoma cell line was switched into a neuronal phenotype by DMSO treatment and used for proteomic experiments. We used two-dimensional gel electrophoresis followed by unambiguous mass spectrometrical identification of proteins to generate a map of cytoskeleton proteins (CPs), i.e., to search for differentiation-related structures. Alpha-actin, actin-like protein 6A, gamma-tubulin complex component 2, tubulin alpha 3/alpha 7, CLIP associating protein 2, B4 integrin interactor homolog were detectable in the undifferentiated cell line exclusively and neuron-specific CPs drebrin and presynaptic density protein 95, actin-related protein 2/3, alpha and beta-centractin, PDZ-domain actin binding protein, actinin alpha 1, profilin II, ezrin, coactosin-like protein, transgelin 2, myosin light polypeptide 6, tubulin alpha 2, 6 and 7, beta tubulin (94% similar with tubulin beta-2), tubulin beta 3, tubulin tyrosine ligase-like protein 1, lamin B1 and keratin 20 were observed in the differentiated cell line only. We herein identified differentiation-related expressional patterns thus providing new evidence for the role of CPs in the process of neuronal differentiation.
Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
J-E
JE
Oh
K
K
Karlmark Raja
J-H
JH
Shin
A
A
Pollak
M
M
Hengstschläger
G
G
Lubec
D000818:Animals; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000032:analysis*; D003599:Cytoskeleton / Q000737:chemistry*; D004121:Dimethyl Sulfoxide; D015180:Electrophoresis, Gel, Two-Dimensional; D051379:Mice; D016501:Neurites / Q000648:ultrastructure; D009447:Neuroblastoma; D009474:Neurons / Q000737:chemistry* / Q000166:cytology* / Q000648:ultrastructure; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003598:Cytoskeletal Proteins; D004121:Dimethyl Sulfoxide