Evaluation of ghee based formulation for wound healing activity.
Formulation containing neomycin and ghee was evaluated for wound-healing potential on different experimental models of wounds in rats. The rats were divided into six groups of group 1 as control, group 2 as treated with neomycin only, group 3 as treated only with ghee, group 4 treated with F-1 formulation containing ghee 40% and neomycin 0.5%, group 5 treated with F-2 formulation containing ghee 50% and neomycin 0.5% and group 6 treated with F-3 formulation containing ghee and ointment base in all two wound models, each group consisting of six rats. Wound contraction ability in excision wound model was measured at different time intervals and study was continued until wound is completely healed. Tensile strength was measured in 10-day-old incision wound and quantitative estimation of hydroxy proline content in the healed tissue was determined in 10-day-old excision wound. Histological studies were done on 10-day-old sections of regenerated tissue of incision wound. F-2 formulation containing ghee 50% and neomycin 0.5% showed statistically significant response, in terms of wound contracting ability, wound closure time, period of epithelization, tensile strength of the wound, regeneration of tissues at wound site when compared with the control group and these results were comparable to those of a reference neomycin ointment.
Department of Pharmaceutical Sciences, Nagpur University, India. vureprasad@gmail.com
Vure
V
Prasad
Avinash Kumar
AK
Dorle
D000818:Animals; D003124:Colorimetry; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D004355:Drug Stability; D005260:Female; D006909:Hydroxyproline / Q000032:analysis; D008297:Male; D009355:Neomycin / Q000008:administration & dosage / Q000494:pharmacology; D009824:Ointments; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength
D004041:Dietary Fats; D009824:Ointments; D009355:Neomycin; D006909:Hydroxyproline