Photosensitizing action of isomeric zinc N-methylpyridylporphyrins in human carcinoma cells.
The success of photodynamic therapy (PDT), as a minimally invasive approach, in treating both neoplastic and non-neoplastic diseases has stimulated the search for new compounds with potential application in PDT. We have previously reported that Zn(II) N-alkylpyridylporphyrins (ZnTM-2(3,4)-PyP(4+) and ZnTE-2-PyP(4+)) can act as photosensitizers and kill antibiotic-resistant bacteria. This study investigated the photosensitizing effects of the isomers of ZnTMPyP(4+) (ZnTM-2(3,4)-PyP(4+)) and respective ligands on a human colon adenocarcinoma cell line. At 10 microM and 30 min of illumination the isomeric porphyrins completely inhibited cell growth, and at 20 microM killed approximately 50% of the cancer cells. All these effects were entirely light-dependent. The isomers of the ZnTMPyP(4+) and the respective ligands show high photosensitizing efficiency and no toxicity in the dark. Their efficacy as photosensitizers is comparable to that of hematoporphyrin derivative (HpD).
Faculty of Medicine, Kuwait University, Department of Biochemistry, Safat.
Dalal A
DA
Al-Mutairi
James D
JD
Craik
Ines
I
Batinic-Haberle
Ludmil T
LT
Benov
D000230:Adenocarcinoma / Q000188:drug therapy / Q000532:radiotherapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000494:pharmacology*; D020033:Protein Isoforms / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D017319:Photosensitizing Agents; D011166:Porphyrins; D020033:Protein Isoforms; D015032:Zinc