Islet recovery and reversal of murine type 1 diabetes in the absence of any infused spleen cell contribution.
A cure for type 1 diabetes will probably require the provision or elicitation of new pancreatic islet beta cells as well as the reestablishment of immunological tolerance. A 2003 study reported achievement of both advances in the NOD mouse model by coupling injection of Freund's complete adjuvant with infusion of allogeneic spleen cells. It was concluded that the adjuvant eliminated anti-islet autoimmunity and the donor splenocytes differentiated into insulin-producing (presumably beta) cells, culminating in islet regeneration. Here, we provide data indicating that the recovered islets were all of host origin, reflecting that the diabetic NOD mice actually retain substantial beta cell mass, which can be rejuvenated/regenerated to reverse disease upon adjuvant-dependent dampening of autoimmunity.
Section on Immunology and Immunogenetics, Joslin Diabetes Center, and Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, and Harvard Stem Cell Institute, 1 Joslin Place, Boston, MA 02215, USA.
Junko
J
Nishio
Jason L
JL
Gaglia
Stuart E
SE
Turvey
Christopher
C
Campbell
Christophe
C
Benoist
Diane
D
Mathis
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015551:Autoimmunity; D001786:Blood Glucose / Q000032:analysis; D002454:Cell Differentiation; D017690:Cell Transplantation; D003131:Combined Modality Therapy; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D005260:Female; D005620:Freund's Adjuvant / Q000627:therapeutic use*; D050417:Insulin-Secreting Cells / Q000166:cytology*; D007515:Islets of Langerhans / Q000166:cytology* / Q000473:pathology; D016381:Islets of Langerhans Transplantation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D020641:Polymorphism, Single Nucleotide; D012038:Regeneration; D013154:Spleen / Q000166:cytology* / Q000276:immunology
D000906:Antibodies; D001786:Blood Glucose; D005620:Freund's Adjuvant