[Study on DNA damage induced by microcystin-LR].
OBJECTIVE
To investigate whether microcystin-LR (MC-LR) at doses which were not cytotoxic can induce DNA damage and compare its effect on HL-7702 and KB cell lines.


METHODS
Cytotoxin and DNA damage were detected by MTT and comet assay, respectively.


RESULTS
As doses ranged from 10 to 100 micro/L, MC-LR showed no significant impact on viability of these two cell lines. However, DNA damage induced by MC-LR occurred at the dose of 30 microg/L in HL-7702 cell and significantly increased with dose. MC-LR did not induce DNA damage in KB cell.


CONCLUSIONS
MC-LR has potential genotoxicity, DNA damage induced by MC-LR is more significant in hepatocyte with bile acid transportation system than other cell lines.
School of Public Health, Fudan University, Shanghai 200032, China.
He-yan
HY
Wu
Jin
J
Su
Wei
W
Shi
D002460:Cell Line; D020552:Comet Assay; D004249:DNA Damage; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D007624:KB Cells; D008387:Marine Toxins; D052998:Microcystins / Q000633:toxicity*
D008387:Marine Toxins; D052998:Microcystins; C057862:cyanoginosin LR