Effects of gentiopicroside, sweroside and swertiamarine, secoiridoids from gentian (Gentiana lutea ssp. symphyandra), on cultured chicken embryonic fibroblasts.
Wound healing properties of Gentian (Gentiana lutea ssp. symphyandra) extract and its main constituents, gentiopicroside, sweroside and swertiamarine (compounds 1-3, respectively) were evaluated by comparison with dexpanthenol on cultured chicken embryonic fibroblasts. The extract was also analyzed by HPLC to quantify its constituents. Chicken embryonic fibroblasts from fertilized eggs were incubated with the plant extract and its constituents, compounds 1-3. Using microscopy, mitotic ability, morphological changes and collagen production in the cultured fibroblasts were evaluated as parameters. Wound healing activity of Gentian seems to be mainly due to the increase in the stimulation of collagen production and the mitotic activity by compounds 2 and 3, respectively (p < 0.005 in all cases). All three compounds also exhibited cytoprotective effects, which may cause a synergism in terms of wound healing activity of Gentian. The findings demonstrated the wound healing activity of Gentian, which has previously been based only on ethnomedical data.
Anadolu University, Faculty of Pharmacy, Department of Pharmacognosy, Eskisehir, Turkey. nozturk@anadolu.edu.tr
Nilgün
N
Oztürk
Seval
S
Korkmaz
Yusuf
Y
Oztürk
K Hüsnü Can
KH
Başer
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003094:Collagen / Q000096:biosynthesis / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects*; D030012:Gentiana; D005960:Glucosides / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D057888:Iridoid Glucosides; D039823:Iridoids / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D011714:Pyrans / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D012879:Skin Physiological Phenomena; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D010936:Plant Extracts; D011714:Pyrans; C012997:gentiopicroside; D003094:Collagen; C049412:sweroside