Maize response to acute arsenic toxicity as revealed by proteome analysis of plant shoots.
Aerial parts (shoots) of maize seedlings fed hydroponically with 300 muM sodium arsenate [As(V)] or 250 muM sodium arsenite [As(III)] for 24 h were analyzed for differentially expressed proteins by 2-DE and digital image analysis. About 15% of total detected proteins (74 out of 500) were up- or, mainly, down-regulated by arsenic, among which 14 were selected as being those most affected by the metalloid. These proteins were analyzed by MALDI-TOF MS and 7 of them were identified: translation initiation factor eIF-5A, ATP synthase, cysteine synthase, malate dehydrogenase, protein kinase C inhibitor, Tn10 transposase-like protein, and guanine nucleotide binding protein. Each of these proteins was completely repressed by As(V) and/or As(III), except protein kinase C inhibitor, which was newly detected after exposure to As(V).
Department of Biochemistry and Molecular Biology, ETSIAM, University of Córdoba, Córdoba, Spain.
Raquel
R
Requejo
Manuel
M
Tena
D001151:Arsenic / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D010940:Plant Proteins / Q000187:drug effects*; D018520:Plant Shoots / Q000187:drug effects*; D020543:Proteome / Q000187:drug effects*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D003313:Zea mays / Q000187:drug effects*
D010940:Plant Proteins; D020543:Proteome; D001151:Arsenic