Three-dimensional structure of the bacterial cell wall peptidoglycan.
The 3D structure of the bacterial peptidoglycan, the major constituent of the cell wall, is one of the most important, yet still unsolved, structural problems in biochemistry. The peptidoglycan comprises alternating N-acetylglucosamine (NAG) and N-acetylmuramic disaccharide (NAM) saccharides, the latter of which has a peptide stem. Adjacent peptide stems are cross-linked by the transpeptidase enzymes of cell wall biosynthesis to provide the cell wall polymer with the structural integrity required by the bacterium. The cell wall and its biosynthetic enzymes are targets of antibiotics. The 3D structure of the cell wall has been elusive because of its complexity and the lack of pure samples. Herein we report the 3D solution structure as determined by NMR of the 2-kDa NAG-NAM(pentapeptide)-NAG-NAM(pentapeptide) synthetic fragment of the cell wall. The glycan backbone of this peptidoglycan forms a right-handed helix with a periodicity of three for the NAG-NAM repeat (per turn of the helix). The first two amino acids of the pentapeptide adopt a limited number of conformations. Based on this structure a model for the bacterial cell wall is proposed.
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556-5670, USA.
Samy O
SO
Meroueh
Krisztina Z
KZ
Bencze
Dusan
D
Hesek
Mijoon
M
Lee
Jed F
JF
Fisher
Timothy L
TL
Stemmler
Shahriar
S
Mobashery
D000595:Amino Acid Sequence; D001419:Bacteria / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure*; D008969:Molecular Sequence Data; D009112:Muramic Acids; D019906:Nuclear Magnetic Resonance, Biomolecular; D009842:Oligopeptides; D010457:Peptidoglycan / Q000737:chemistry* / Q000648:ultrastructure*; D011487:Protein Conformation
D009112:Muramic Acids; D009842:Oligopeptides; D010457:Peptidoglycan; C031651:N-acetylmuramic acid