One-step concentration and partial purification of Aspergillus kawachii non-acidic polygalacturonases by adsorption to glass fiber microfilters.
The non-acidic polygalacturonases produced by Aspergillus kawachii in a glucose/tryptone medium were adsorbed to a glass fiber microfilter that was used to clarify the fermentation broth. Maximum adsorption occurred at pH 3 under low ionic strength conditions. The adsorbed activity could be readily released with a buffer solution at pH 5. Based upon these observations, a separation process was developed which enabled the broth to be clarified and, at the same time, the non-acidic polygalacturonases to be concentrated 20-fold and purified 100-fold in a unique filtration step. The practical advantage of recovering polygalacturonases by a filtration process lies in the simplicity and efficiency of the operation involved.
CINDEFI, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Conicet 47 y 115 (1900), La Plata, Argentina. voget@biotec.org.ar
Claudio E
CE
Voget
Carolina E
CE
Vita
Juan C
JC
Contreras Esquivel
D000327:Adsorption; D001230:Aspergillus / Q000201:enzymology*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media / Q000302:isolation & purification; D005898:Glass / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011096:Polygalacturonase / Q000737:chemistry* / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000295:instrumentation* / Q000379:methods
D003470:Culture Media; C009753:fiberglass; D011096:Polygalacturonase