[Human cytomegalovirus induces apoptosis of ECV304 endothelial-like cells].
OBJECTIVE
To investigate the mechanisms for the cytopathic effect (CPE) of human cytomegalovirus (HCMV) in ECV304 endothelial-like cells.


METHODS
PCR and indirect immunofluorescence were used to detect HCMV infection by examining immediate-early (IE) gene and protein expression of the virus in ECV304 cells. Phase-contrast and electron microscopies were performed to observe the morphological changes of the infected and uninfected cells, and DNA ladder analysis and flow cytometry were carried out to study HCMV-induced cell apoptosis.


RESULTS
In HCMV-infected ECV304 cells, cytopathic effects were first observed at approximately 72 h post-infection. The cells with CPE changes exhibited detachment from the monolayer, cell rounding and shrinkage. The expression of the IE gene was detected. Chromatin condensation and nuclear fragmentation along with dramatic changes of the mitochondria were observed by electron microscopy at 96 h post-infection. Cellular DNA fragmentation was observed in the infected cells, which had cells apoptotic rates of 4.1% and 45.7% at 96 h and 144 h post-infection, respectively.


CONCLUSIONS
HCMV can induce apoptosis of ECV304 endothelial-like cells.
Institute of Genetical Engineering, Southern Medical University, Guangzhou 510515, China. zhangyl20011002@yahoo.com.cn
Ya-li
YL
Zhang
Wen-li
WL
Ma
Xiao-Yang
XY
Mo
Hai-quan
HQ
Zhao
Chang-wen
CW
Ke
Huan-ying
HY
Zheng
Wen-ling
WL
Zheng
D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis; D042783:Endothelial Cells / Q000166:cytology* / Q000648:ultrastructure / Q000821:virology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D016133:Polymerase Chain Reaction; D014471:Umbilical Veins / Q000166:cytology / Q000821:virology
D000956:Antigens, Viral; D004279:DNA, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus