A surfactant copolymer facilitates functional recovery of heat-denatured lysozyme.
The triblock copolymer poloxamer 188 is a non-cytotoxic, nonionic surfactant with both hydrophobic and hydrophilic domains. We show that P188 is able to facilitate the recovery of catalytic activity of heat-denatured lysozyme in dilute solution at low molar ratios of P188:enzyme. Heat-denatured enzyme retained 55% of native activity. After treatment with P188, the enzyme's activity was 85% of native. Because of the low molar ratios used and the non-cytotoxic nature of the compound, P188 may be of potential use in burn therapy.
Department of Surgery, University of Chicago Hospitals, MC 6035, 5841 S. Maryland Ave., Chicago, IL 60637, USA.
Alexandra M
AM
Walsh
Devkumar
D
Mustafi
Marvin W
MW
Makinen
Raphael C
RC
Lee
D000818:Animals; D002645:Chickens; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism*; D020442:Poloxamer / Q000737:chemistry / Q000494:pharmacology*; D011489:Protein Denaturation; D020673:Protein Renaturation
D020442:Poloxamer; C421864:hen egg lysozyme; D009113:Muramidase