Plaque reduction technique for demonstrating neutralizing antibodies for Chlamydia.
Some members of the genus Chlamydia (Bedsonia or psittacosis-lymphogranuloma venereum-trachoma-inclusion conjunctivitis group of microorganisms) produce plaques in monolayers of mouse fibroblast (L-929) cells. Hyperimmune chicken antisera may be capable of specifically reducing plaque counts. When the test was applied to chlamydiae isolated from avian species, the results indicated that different isolates with a common source had similar antigenic reactivity. The plaque reduction test is a potentially useful method for serotyping chlamydiae. The difficult aspect of the method appears to be a readily reproducible means of producing neutralizing antiserum.
The G. W. Hooper Foundation, University of California, San Francisco, San Francisco, California 94122.
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Banks
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Eddie
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Sung
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Sugg
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Schachter
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Meyer