Evaluation of a double-setting alpha-tricalcium phosphate cement in eviscerated rabbit eyes.
OBJECTIVE
To evaluate the macroscopy, microstructure, and tissue reaction of a double-setting alpha-tricalcium phosphate bone cement used as an intraocular implant in rabbits.


METHODS
The internal and external surface of the double-setting alpha-tricalcium phosphate implant was analyzed macroscopically and by scanning electron microscopy. Twelve New Zealand rabbits received 12-mm implants made of double-setting alpha-tricalcium phosphate cement after unilateral evisceration. Clinical evaluation was performed daily for the first 15 days after surgery and at 15-day intervals until the end of the study period. For histopathologic analysis, 3 animals per experimental period were submitted to enucleation at 15, 45, 90, and 180 days.


RESULTS
On gross inspection, the external surface of the implant was solid, smooth, and compact. The microarchitecture was characterized by the formation of columns of hexagonal crystals with interconnecting channels forming micropores. No wound dehiscence, signs of infection, or implant extrusion were observed in any animal throughout the study period. Histologic examination revealed the formation of fibrovascular tissue surrounding the implants, and there were signs of minimal integration of the surface limiting the fibrocellular cap with the space previously occupied by the implant.


CONCLUSIONS
The double-setting alpha-tricalcium phosphate implant behaved as an inert and non-integratable material. The lack of incorporation of this material by fibrovascular tissue is related to its characteristics of compactness and high resistance.
Oriá
Arianne P
AP
Veterinary College, São Paulo State University, UNESP, Jaboticabal, São Paulo, Brazil.
Neto
Francisco A Dórea
FA
Laus
José L
JL
Dos Santos
Luis A
LA
Piza
Evandro T
ET
Brunelli
Adriana T
AT
Nishimori
Celina T
CT
de Souza
Ana Letícia G
AL
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001843:Bone Cements / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000627:therapeutic use*; D004195:Disease Models, Animal; D017234:Eye Evisceration; D005500:Follow-Up Studies; D008855:Microscopy, Electron, Scanning; D009915:Orbit / Q000473:pathology / Q000601:surgery; D020273:Orbital Implants; D011474:Prosthesis Design; D019919:Prosthesis Implantation / Q000379:methods*; D011817:Rabbits; D013499:Surface Properties
D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate