Effects of Pouteria cambodiana extracts on in vitro immunomodulatory activity of mouse immune system.
Immunomodulatory activity of water and acetone extracts of stem bark of Pouteria cambodiana was examined on murine macrophage phagocytosis [nitroblue tetrazolium (NBT) dye reduction and lysosomal enzyme activity] and proliferation of splenocytes and bone marrow cells by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay with and without lipopolysaccharide (LPS) or pokeweed mitogen (PWM). Both aqueous and acetone extracts presented immunomodulatory activity without clear dose response relationship.
Faculty of Pharmacy, Chiang Mai University, Thailand. pmpti005@chiangmai.ac.th
Aranya
A
Manosroi
Aurasorn
A
Saraphanchotiwitthaya
Jiradej
J
Manosroi
D000096:Acetone / Q000737:chemistry; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D032132:Pouteria / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014867:Water / Q000737:chemistry
D007155:Immunologic Factors; D008934:Mitogens; D010936:Plant Extracts; D014867:Water; D000096:Acetone