Detection of virulence determinants in clinical strains of Salmonella enterica serovar Enteritidis and mapping on macrorestriction profiles.
A total of 80 strains of Salmonella enterica serovar Enteritidis, causing gastroenteritis (G) or bacteraemia (B), and three control strains (C), were subjected to: (i) detection of 14 chromosomally and 1 plasmid-located virulence genes by PCR, (ii) detection of DNA polymorphisms by XbaI and BlnI PFGE, and cluster analysis, (iii) mapping of the 15 screened sequences on macrorestriction profiles and (iv) comparison of the screening and mapping results with data available for other Salmonella strains. Identical virulence genotypes and very similar macrorestriction profiles were shown by most S. Enteritidis strains. However, a number of B strains belonged to genomic types with polymorphisms affecting fragments carrying (SPI2-slyA), (SPI2-slyA-phoP/Q-agfA), (SPI4 and/or stn) and spvC. The information obtained provides the basis for further studies on the genetic background of virulence and the molecular epidemiology of S. Enteritidis.
Servei de Microbiología, Centre de Diagnòstic Biologic, Hospital Clinic-IDIBAPS, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain.|Departamento de Biología Funcional, Área de Microbiología, Universidad de Oviedo, C/Julián Clavería 6, 33006 Oviedo, Spain.
Sara M
SM
Soto
Departamento de Biología Funcional, Área de Microbiología, Universidad de Oviedo, C/Julián Clavería 6, 33006 Oviedo, Spain.
Irene
I
Rodríguez
Departamento de Biología Funcional, Área de Microbiología, Universidad de Oviedo, C/Julián Clavería 6, 33006 Oviedo, Spain.
M Rosario
MR
Rodicio
Servei de Microbiología, Centre de Diagnòstic Biologic, Hospital Clinic-IDIBAPS, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain.
Jordi
J
Vila
Departamento de Biología Funcional, Área de Microbiología, Universidad de Oviedo, C/Julián Clavería 6, 33006 Oviedo, Spain.
M Carmen
MC
Mendoza
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics