Cloning of TBX5, a key gene during heart formation and its expression in rat embryonic heart.
To study the expression of TBX5, a key gene during heart formation, full-length TBX5 cDNA was cloned from Wistar rat embryonic heart (GenBank Accession No. AY859491). The cDNA and predicted amino acid sequences were different from those previously reported in GenBank. The expression profile of TBX5 in rat tissues was studied by RT-PCR and Northern blot. TBX5 was expressed in many rat tissues as a single transcript, and the highest level of expression was found in the heart. For subcellular localization, TBX5 was expressed as an EGFP fusion protein in rat hepatocarcinoma cells. Results showed that TBX5 was nuclear. In addition, TBX5-GST fusion protein was obtained by prokaryotic expression. These findings provide a good basis for further identification of TBX5-related transcription factors and protein-protein interaction studies among each other.
Gong
Li-Guo
LG
Department of Medical Genetics, China Medical University, Shenyang 110001, China. gonglig@sina.com
Qiu
Guang-Rong
GR
Xin
Na
N
Xu
Xiao-Yan
XY
Sun
Kai-Lai
KL
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006321:Heart / Q000196:embryology; D007438:Introns; D008969:Molecular Sequence Data; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D020825:T-Box Domain Proteins; C103172:T-box transcription factor 5; D005982:Glutathione Transferase