[Study on HPLC determination method and pharmacokinetics of tirapazaming in rabbits].
We have developed an HPLC method for the determination of tirapazaming (3-Amino-1,2,4-Benzotriazine -1,4-dioxide, TPZ) in rabbits and have studied the related pharmacokinetics in rabbits. After protein was precipitated, TPZ in plasma was determinated on HPLC with UV detection at 266 nm, and the mobile phase consisted of methyl alcohol and phosphoric buffer (10: 90, adjusted to pH6.5 +/- 0.1 with NaOH). TPZ and endogenous substances could be separated. Its calibration curve was linear (r=0.9995) within the range of 0.150-59. 98 mg/L, the detection limits was 0.5 ng (S/N>3), the recovery was more than 95% (n=3), the intra-day and inter-day precision was less than 6.0% (n = 5). The concentration-time curve of TPZ in rabbit plasma could be fitted to two-compartment model. These data indicate the method is sensitive, simple and reliabe. It is suitable for the pharmacokinetic study of TPZ in rabbits.
West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Hong
H
Chen
Dan
D
Zhang
Qingyuan
Q
Deng
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D011817:Rabbits; D011838:Radiation-Sensitizing Agents / Q000493:pharmacokinetics*; D012680:Sensitivity and Specificity; D000077704:Tirapazamine; D014227:Triazines / Q000097:blood* / Q000493:pharmacokinetics*
D011838:Radiation-Sensitizing Agents; D014227:Triazines; D000077704:Tirapazamine