Development of new simple molecular probes of DNA bulged structures.
NCSi-gb is a neocarzinostatin chromophore (NCS-chrom) metabolite which binds strongly to certain two-base DNA bulges. Compared with previously reported NCSi-gb analogues, a new analogue with a different aminoglycoside position was synthesized, and it showed strong fluorescence and improved binding and sequence selectivity to DNA bulges. The N-dimethylated form of this analogue had a similar binding pattern, and it competitively inhibited bulge-specific cleavage by NCS-chrom.
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Ziwei
Z
Xiao
Lizzy S
LS
Kappen
Irving H
IH
Goldberg
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry*; D015394:Molecular Structure
D015335:Molecular Probes; D004247:DNA