Large-scale gene discovery in the pea aphid Acyrthosiphon pisum (Hemiptera).
Aphids are the leading pests in agricultural crops. A large-scale sequencing of 40,904 ESTs from the pea aphid Acyrthosiphon pisum was carried out to define a catalog of 12,082 unique transcripts. A strong AT bias was found, indicating a compositional shift between Drosophila melanogaster and A. pisum. An in silico profiling analysis characterized 135 transcripts specific to pea-aphid tissues (relating to bacteriocytes and parthenogenetic embryos). This project is the first to address the genetics of the Hemiptera and of a hemimetabolous insect.
Sabater-Muñoz
Beatriz
B
INRA Rennes, UMR INRA-Agrocampus BiO3P, BP 35327, F-35653 Le Rheu Cedex, France.
Legeai
Fabrice
F
Rispe
Claude
C
Bonhomme
Joël
J
Dearden
Peter
P
Dossat
Carole
C
Duclert
Aymeric
A
Gauthier
Jean-Pierre
JP
Ducray
Danièle Giblot
DG
Hunter
Wayne
W
Dang
Phat
P
Kambhampati
Srini
S
Martinez-Torres
David
D
Cortes
Teresa
T
Moya
Andrès
A
Nakabachi
Atsushi
A
Philippe
Cathy
C
Prunier-Leterme
Nathalie
N
Rahbé
Yvan
Y
Simon
Jean-Christophe
JC
Stern
David L
DL
Wincker
Patrick
P
Tagu
Denis
D
D000818:Animals; D001042:Aphids / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D018895:Microsatellite Repeats; D018532:Pisum sativum / Q000469:parasitology; D010802:Phylogeny; D010935:Plant Diseases / Q000469:parasitology; D011156:Population Density; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004247:DNA