A Method for Purification of Clostridium perfringens Phospholipase C from Recombinant Bacillus subtilis Cells.
We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.
Y
Y
Hirata
J
J
Minami
M
M
Koyama
O
O
Matsushita
S
S
Katayama
F
F
Jin
H
H
Maeta
A
A
Okabe