Synthesis and characterization of poly(L-lactic acid-co-ethylene oxide-co-aspartic acid) and its interaction with cells.
Multiblock terpolymer of poly(L-lactic acid)/poly(ethylene oxide)/poly(L-aspartic acid), (PLLA/PEO/PAsp) was synthesized by ring opening polymerization of beta -benzyl L-aspartate N-carboxyanhydride, Asp(OBzl)-NCA with alpha-omega -hydroxy terminated triblock PLLA/PEO/PLLA copolymer. The resulting multiblock terpolymer was characterized by several techniques including Fourier transform infrared spectroscopy and differential scanning calorimetry.(1)H nuclear magnetic resonance spectra indicated the molar ratio of PLLA/PEO/PAsp (OBzl) to be 86/10/4. Thermal gravimetric analysis and environmental scanning electron microscopy data showed that PLLA/PEO/PAsp had crystalline and brittle structure. In order to improve its mechanical and physical properties, the terpolymer was blended with high molecular weight poly(L-lactic-co-glycolic acid) copolymer, PLGA(85/15) (M(w): 95000 gmol(-1)) in 25/75 and 50/50 mole ratios. The hydrolytical degradation properties of these polymers were studied. Degradation experiments were performed during a 48-day period in pH:7.4 phosphate-buffered saline (PBS) at 37 degrees C. The observed molecular weight losses were 91% and 67% for the 25/75 and 50/50 mixtures, respectively. In vitro attachment and growth of L929 mouse fibroblasts on these biopolymers were also investigated. Cell growth experiments indicated that the copolymer blend allowed the attachment and growth of cells.
Karal-Yilmaz
Okşan
O
TUBITAK, Research Institute for Genetic Engineering and Biotechnology, Gebze-Kocaeli, Turkey.
Kayaman-Apohan
Nilhan
N
Misirli
Zülal
Z
Baysal
Kemal
K
Baysal
Bahattin M
BM
D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry*; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D001673:Biodegradation, Environmental / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008958:Models, Molecular; D011100:Polyglycolic Acid / Q000138:chemical synthesis* / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000138:chemical synthesis* / Q000494:pharmacology; D013499:Surface Properties; D013696:Temperature; D023822:Tissue Engineering
D001672:Biocompatible Materials; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D001224:Aspartic Acid; D019344:Lactic Acid