Determination of cholesterol in human hair using gas chromatography-mass spectrometry.
This work describes a sensitive method for determining cholesterol in human hair using GC-MS. In this study, we used a very small amount of hair, only 1 mg, to quantify cholesterol. We also can achieve more effective purification and a good recovery over 92% with solid-phase extraction using an Oasis HLB cartridge. The intra-day and inter-day precision and accuracy values were less than 7.08%. Cholesterol was determined to be in the range of 355-1693 microg/g in healthy human hair. We tested the concentration correlation between the serum and hair to examine the feasibility of using the hair cholesterol level as an index of the serum cholesterol level. The correlation between the serum cholesterol was 0.86 (r-value) in patients with hypercholesterolemia. This finding indicates that, in the clinical field, hair could replace serum in cholesterol level measurement.
Bioanalysis and Biotransformation Research Center, KIST, Chengryang, Seoul, 130-605, Korea.
Hye Kyung
HK
Ryu
Byung Hwa
BH
Jung
Kyung Mee
KM
Kim
Eun Ah
EA
Yoo
Jeong-Taek
JT
Woo
Bong Chul
BC
Chung
D000328:Adult; D002784:Cholesterol / Q000032:analysis* / Q000097:blood; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results
D002784:Cholesterol