The degree of lymphocytic mitochondrial transmembrane potential and blood magnesium concentrations during coronary artery bypass grafting.
Magnesium (Mg) plays an important role in lymphocyte function. Low blood concentration of Mg may result in intralymphocyte imbalance and in turn may be associated with intensified apoptosis of peripheral blood lymphocytes. Due to its multistage character; extracorporeal circulation (ECC) may augment Mg disorders adding to the above mentioned pathology. The aim of this study was to assess the correlation between lymphocyte apoptosis and Mg concentration in the blood during the course of coronary artery bypass grafting (CABG) and in the early postoperative period.


METHODS
Twenty male patients undergoing CABG with ECC under general anaesthesia were included in the study. For detection of apoptotic lymphocytes in the circulation, inner mitochondrial transmembrane potential (deltapsim) was measured with the use of chloromethyl-X-rosamine (CMXRos) and flow cytometry. Spectrophotometry was used for Mg blood concentration measurements. Peripheral blood samples were obtained in seven stages: 1) just before anaesthesia, 2) 2 hours after the beginning of surgery, 3) immediately after surgery, 4) 12 hours after the beginning of surgery, 5) 24 hours after the beginning of surgery, 6) 36 hours after the beginning of surgery, 7) 54 hours after the beginning of surgery.


RESULTS
The statistically significant increases of lymphocyte apoptosis were noted in stages from 2 to 7. Blood Mg concentrations decreased in stages 2 and 3. There was negative correlation between Mg blood concentration in stages 2 and 3 and the intensity of lymphocyte apoptosis in the stage 5.


CONCLUSIONS
1) CABG with extracorporeal circulation was associated with a decrease of magnesium concentration in the blood and an increase of lymphocyte apoptosis intensity. 2) The decrease of magnesium blood concentration may increase the degree of lymphocyte apoptosis. 3) Lymphocyte apoptosis after extracorporeal circulation has a two-phase course.
Korycińska
A
A
Department of Clinical Immunology Medical, Feliks Skubiszewski Medical University of Lublin, Poland.
Dabrowski
W
W
Rzecki
Z
Z
Dragan
M
M
Pozarowski
P
P
Wrońska
J
J
Stazka
J
J
Pasternak
K
K
Roliński
J
J
D000368:Aged; D017209:Apoptosis / Q000502:physiology; D001026:Coronary Artery Bypass; D005112:Extracorporeal Circulation; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology; D008274:Magnesium / Q000097:blood*; D008297:Male; D008564:Membrane Potentials / Q000502:physiology*; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D011184:Postoperative Period; D013223:Statistics as Topic
D008274:Magnesium