[Correlation of expression of nuclear factor kappaB, inhibitor protein kappaB, and Bcl-2 in cervical cancer to human papillomavirus infection].
OBJECTIVE
It was proved that nuclear factor kappaB (NF-kappaB) and its inhibitor protein kappaB (I-kappaB) played critical roles in regulating the expression of proapoptotic genes, and NF-kappaB is overexpressed in some tumors and related with tumorigenesis. However, the expression of NF-kappaB and I-kappaB in cervical cancer and its correlation to human papillomavirus (HPV) infection have not been reported yet. This study was to explore the correlation of the expression of NF-kappaB, I-kappaB, and Bcl-2 in cervical cancer to human papillomavirus (HPV) infection.


METHODS
The expression of NF-kappaB, I-kappaB, and Bcl-2 were detected by immunohistochemistry in 46 specimens of cervical cancer and 26 specimens of normal cervical tissue. HPV DNA was detected by polymerase chain reaction (PCR).


RESULTS
The positive rates of NF-kappaB and Bcl-2 were significantly higher in cervical cancer than in normal cervical tissue (60.9% vs. 23.1%, 52.2% vs. 0.0%, P < 0.01). The positive rate of I-kappaB was significantly lower in cervical cancer than in normal cervical tissue (30.4% vs. 57.7%, P < 0.05). The expression of NF-kappaB was closely related to Bcl-2 (P < 0.05). The positive rate of NF-kappaB was significantly higher in HPV DNA-positive cervical cancer than in HPV DNA-negative cervical cancer (81.3% vs. 35.7%, P < 0.05), but the positive rates of I-kappaB and Bcl-2 between these 2 groups had no significant difference (P >0.05).


CONCLUSIONS
The high expression of NF-kappaB and Bcl-2 and the low expression of I-kappaB may be related to cervical carcinogenesis, and NF-kappaB expression may be related to HPV infection.
Department of Microbiology and Immunology, Wenzhou Medical College, Wenzhou, Zhejiang, 325027, PR China. wyxkd@wzmc.net
Ke-Dong
KD
Xia
Xiang-Min
XM
Chen
Qiao-Ai
QA
Lin
Hai-Yan
HY
Dong
Shao
S
Chen
Li-Fang
LF
Zhang
D000328:Adult; D000368:Aged; D002278:Carcinoma in Situ / Q000378:metabolism / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism*; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism*; D030361:Papillomavirus Infections; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000821:virology
D004279:DNA, Viral; D020672:I-kappa B Proteins; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2