The value of autofluorescence as a diagnostic feature of acute promyelocytic leukemia.
Autofluorescence is an immunophenotypic characteristic of leukemic blasts in acute promyelocytic leukemia (APL). We examined the fluorescent intensity of isotype controls in 25 cases of APML and 25 controls with acute myeloid leukemia. The fluorescence of the FITC- and PE-conjugated controls was consistently higher in APML. Autofluorescence may therefore represent a helpful diagnostic marker in APML.
Hayden
Patrick J
PJ
O'Connell
Niamh M
NM
O'Brien
David A
DA
O'Rourke
Paul
P
Lawlor
Emer
E
Browne
Paul V
PV
D001752:Blast Crisis / Q000175:diagnosis / Q000473:pathology; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D005434:Flow Cytometry / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D016130:Immunophenotyping; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis* / Q000473:pathology