The effect of interleukin-17 on the proliferation and invasion of JEG-3 human choriocarcinoma cells.
OBJECTIVE
As there has been a study in mice showing the expression of IL-17 by decidual cells and the status of IL-17 receptor expression in human pregnancy is not known, we hypothesized that IL-17 may regulate human trophoblast proliferation and invasion.


METHODS
JEG-3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL-17 receptor protein and mRNA, respectively. The effects of IL-17 on JEG-3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively.


RESULTS
IL-17 increased the invasive capacity of JEG-3 cells but had no effect on the proliferation and multinucleated formation of JEG-3 cells.


CONCLUSIONS
In this JEG-3 cell model of human trophoblast, the IL-17R and IL-17 may have a regulatory role in trophoblast invasion.
Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand. sutatipp@nu.ac.th
Sutatip
S
Pongcharoen
Pannika
P
Niumsup
Donruedee
D
Sanguansermsri
Kwansuda
K
Supalap
Puntharee
P
Butkhamchot
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002822:Choriocarcinoma / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D020381:Interleukin-17 / Q000502:physiology*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000096:biosynthesis; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D053722:Receptors, Interleukin-17; D014327:Trophoblasts / Q000276:immunology / Q000378:metabolism / Q000473:pathology*
C109985:IL17RA protein, human; D020381:Interleukin-17; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053722:Receptors, Interleukin-17