[Growth of compound layer tissue engineered oral mucosa and its clinical application in hetero-transplantation].
OBJECTIVE
To investigate the growth of the tissue engineered mucosa after its hetero-transplantation.


METHODS
The epithelial cells and fibroblasts were isolated from a postoperative tissue of the 3-month patient with labial cleft. The epithelial cells and fibroblasts were separately seeded on the polylactic/glycolic acid copolymer membrane, and then they were exposed to the air-liquid interface. Seven volunteer patients, whose traumatic beds were repaired with the tissue engineered oral mucosa. The biopsy tissue from one of the seven patients was observed under light microscope 18 and 30 days after transplantation, respectively.


RESULTS
The tissue engineered oral mucosa having 5-6 layers anti-cytokeratin staining positively cells in the epithelial layer and 3-7 layers anti-Cytokeratin staining negatively cells in the subepithelial layer grew well after the hetero-transplantation. No difference could be found between the transplanting and normal areas. At 18 days, the epithelial layer and lamina propria grew well and the fibroblasts were found; at 30 days, collagen was obviously observed. The structures in both the transplanting and the normal areas were similar.


CONCLUSIONS
The tissue engineered oral mucosa can grow well after the hetero-transplantation.
Li
Hongwei
H
Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology of Nanjing Medical University Jiangsu, Nanjing 210029, P R China. lhwqhxa@sina.com
Zhang
Donghua
D
Wen
Yuming
Y
Wang
Changmei
C
D000293:Adolescent; D000328:Adult; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002648:Child; D004847:Epithelial Cells / Q000166:cytology / Q000637:transplantation; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000637:transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology* / Q000637:transplantation*; D023822:Tissue Engineering; D014184:Transplantation, Homologous