Interferon-beta antibodies have a higher affinity in patients with neutralizing antibodies compared to patients with non-neutralizing antibodies.
In this study, we investigated the affinity, determined by a relative affinity assay, using increasing concentrations of sodium-isothiocyanate to disrupt the antigen antibody binding of neutralizing and non-neutralizing antibodies against interferon-beta (IFNbeta)-1a and -1b in 73 serum samples of MS patients treated with IFNbeta-1a or -1b. Relative affinity values were significantly higher in NAB-positive compared to NAB-negative samples and in samples of IFNbeta-1a-treated patients compared to IFNbeta-1b. A significant positive correlation between relative affinity values and therapy duration indicates affinity maturation as another qualitative factor in IFNbeta neutralization.
Gneiss
C
C
Department of Neurology, Innsbruck Medical University, Anichstrasse 35, 6020 Innsbruck, Austria.
Tripp
P
P
Ehling
R
R
Khalil
M
M
Lutterotti
A
A
Egg
R
R
Mayringer
I
I
Künz
B
B
Berger
T
T
Reindl
M
M
Deisenhammer
F
F
D000704:Analysis of Variance; D000906:Antibodies / Q000502:physiology / Q000627:therapeutic use*; D001667:Binding, Competitive / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D016899:Interferon-beta / Q000276:immunology* / Q000378:metabolism; D009103:Multiple Sclerosis / Q000276:immunology / Q000628:therapy*; D009500:Neutralization Tests / Q000379:methods; D013997:Time Factors; D016896:Treatment Outcome
D000906:Antibodies; D016899:Interferon-beta