Effect of pressure cooking on aflatoxin B1 in rice.
The effect of pressure cooking on aflatoxin residues in polished rice was conducted to determine reduction of aflatoxin and mutagenic potentials. Three rice lots consisting of naturally contaminated, A. parasiticus-infested, and aflatoxin-spiked rice were steamed by ordinary and pressure cookers after they were washed with water. They were chemically analyzed for aflatoxins using a silica solid phase extraction tube and high-performance liquid chromatography (HPLC)-fluorescence detection (FD), and the presence of aflatoxin residues was confirmed using HPLC-electrospray ionization (ESI)-mass spectrometry (MS). An in vitro mutagenicity test with Salmonella typhimurium TA100 was employed to verify the results based on chemical analyses. The aflatoxin loss (78-88%) was notable after pressure cooking, and the reduction of aflatoxin-induced mutagenic potential (68-78%) was in good agreement with the HPLC results. It can be concluded that Koreans are safe from the aflatoxin-related risk if a pressure cooker is employed for cooking rice. The average Korean daily intake of aflatoxin through the consumption of staple rice would fall to 0.15 ng/kg bw/day, which would not exceed the established tolerable daily intake (0.40 ng/kg bw/day).
Park
Je Won
JW
The Graduate School of Biotechnology, Korea University, Seoul 136-701, Republic of Korea. jewonpark@hotmail.com
Kim
Young-Bae
YB
D016604:Aflatoxin B1 / Q000032:analysis* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D006358:Hot Temperature; D007723:Korea; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology; D012275:Oryza / Q000737:chemistry*; D011312:Pressure; D021241:Spectrometry, Mass, Electrospray Ionization
D009153:Mutagens; D016604:Aflatoxin B1