A surfactant copolymer facilitates functional recovery of heat-denatured lysozyme.
The triblock copolymer poloxamer 188 is a non-cytotoxic, nonionic surfactant with both hydrophobic and hydrophilic domains. We show that P188 is able to facilitate the recovery of catalytic activity of heat-denatured lysozyme in dilute solution at low molar ratios of P188:enzyme. Heat-denatured enzyme retained 55% of native activity. After treatment with P188, the enzyme's activity was 85% of native. Because of the low molar ratios used and the non-cytotoxic nature of the compound, P188 may be of potential use in burn therapy.
Walsh
Alexandra M
AM
Department of Surgery, University of Chicago Hospitals, MC 6035, 5841 S. Maryland Ave., Chicago, IL 60637, USA.
Mustafi
Devkumar
D
Makinen
Marvin W
MW
Lee
Raphael C
RC
D000818:Animals; D002645:Chickens; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism*; D020442:Poloxamer / Q000737:chemistry / Q000494:pharmacology*; D011489:Protein Denaturation; D020673:Protein Renaturation
D020442:Poloxamer; C421864:hen egg lysozyme; D009113:Muramidase