Dual polarization interferometry characterization of carbohydrate-protein interactions.
Dual polarization interferometry (DPI) is an analytical technique that allows the simultaneous determination of thickness, density, and mass of a biological layer on a sensing waveguide surface in real time. The technique was applied to the analysis of carbohydrate-protein interactions. The selected system involved a 12-kDa recombinant fragment of collagen V (HepV) and heparin, a complex polysaccharide. Here we report on the analysis of thickness, density, and mass of surface structures obtained during the binding of HepV to heparin, which is a useful model compound for the sulfated, protein-binding regions of heparan sulfate. This system, which was initially studied for its biological relevance, displayed anomalous behavior in kinetic studies using surface plasmon resonance (SPR) assays that has been attributed to putative conformational changes. It was this putative conformational change that prompted us to investigate the binding using an alternative analytical approach. While using DPI to monitor binding events, a streptavidin layer (surface coverage 2.105 ng mm(-2)) was bound to the sensor surface (92% coverage), which captured 0.105 ng mm(-2) of biotinylated heparin (a stoichiometric ratio of 1:6 heparin-streptavidin). The heparin inserted into the streptavidin layer but was still found to be capable of binding 0.154 ng mm(-2) of HepV, which was also observed to insert into the streptavidin layer. This allowed the reliable calculation of the stoichiometric ratio for the HepV-heparin complex ( approximately 1.7:1.0), which has proved to be difficult to evaluate by SPR assays. Furthermore, real-time analysis of the heparin-HepV interaction by DPI suggested that there was some surface loss (probably of streptavidin) while the binding was occurring rather than the putative conformational change that has been suggested on the basis of kinetic data alone. This gives further insight into the binding mechanism of HepV to heparin.
Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS-UCBL, 7 passage du Vercors 69367, Lyon cedex 07, France.
Sylvie
S
Ricard-Blum
Louise L
LL
Peel
Florence
F
Ruggiero
Neville J
NJ
Freeman
D001665:Binding Sites; D019921:Biotinylation; D024062:Collagen Type V / Q000032:analysis / Q000737:chemistry*; D006493:Heparin / Q000032:analysis / Q000737:chemistry*; D007368:Interferometry / Q000379:methods; D007700:Kinetics; D011485:Protein Binding; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods; D013499:Surface Properties; D013997:Time Factors
D024062:Collagen Type V; D011994:Recombinant Proteins; D006493:Heparin; D019809:Streptavidin