A simplified LC-DAD method with an RP-C12 column for routine monitoring of three sulfonamides in edible calf and pig tissue.
Use of an RP-C(12) analytical column with a mobile phase at pH 4.5 enabled excellent chromatographic separation and quantification of sulfadiazine (SDZ), sulfamerazine (SMR), and sulfamethazine (SMZ) in edible calf and pig tissue (muscle and liver). Tissue samples were extracted with acetonitrile, centrifuged without further clean-up, and analyzed by LC-DAD. The proposed conditions are useful for a rapid and reliable screening of the SDZ, SMR, and SMZ content of calf and pig tissue at concentrations lower than the maximum residue level (MRL) (LOQ between 27 and 55 ppb). Separation of some possible SMZ metabolites should be further investigated.
Gratacós-Cubarsí
M
M
Institute for Food and Agricultural Research and Technology, Meat Technology Centre (IRTA-CTC), Granja Camps i Armet s/n, Monells, 17121, Girona, Spain.
Castellari
M
M
Valero
A
A
García-Regueiro
J A
JA
D000818:Animals; D002417:Cattle; D002845:Chromatography / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D008460:Meat / Q000032:analysis*; D013411:Sulfadiazine / Q000032:analysis; D013416:Sulfamerazine / Q000032:analysis; D013418:Sulfamethazine / Q000032:analysis; D013424:Sulfanilamides / Q000032:analysis*; D013552:Swine
D013424:Sulfanilamides; D013411:Sulfadiazine; D013418:Sulfamethazine; D013416:Sulfamerazine