Tissue specificity of methylation of cytosines in regulatory regions of four genes located in the locus FXYD5-COX7A1 of human chromosome 19: correlation with their expression level.
In this study, we compared degree of methylation of selected CpG sites in CCGG sequences located in promoter regions of four human genes with expression level of these genes in several human cell lines and tissues. These genes were subdivided into two groups according to the dependence of their expression on CpG methylation in the 5 -regions. The first group, characterized by clear correlation of methylation with the transcription level, includes housekeeping gene COX6B (the absence of methylation unambiguously correlates with expression) and urothelium-specific uroplakin gene (the methylation coincides with absence of expression). The second group includes genes that are expressed in many, but not all tissues and cells. For these genes (LEAP-1 and ATP4A), there was no correlation between methylation and expression. It is possible that methylation provides some basal level of gene repression, which is overcome by binding of tissue-specific transcription factors, whereas lack of methylation gives the opportunity for gene expression in various cells and tissues.
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
T V
TV
Chalaya
S B
SB
Akopov
L G
LG
Nikolaev
E D
ED
Sverdlov
D000251:Adenosine Triphosphatases / Q000235:genetics; D023181:Antimicrobial Cationic Peptides / Q000235:genetics; D002460:Cell Line; D002888:Chromosomes, Human, Pair 19; D018899:CpG Islands; D003596:Cytosine / Q000737:chemistry*; D003576:Electron Transport Complex IV / Q000235:genetics; D015870:Gene Expression; D064451:Hepcidins; D006801:Humans; D007473:Ion Channels; D008562:Membrane Glycoproteins / Q000235:genetics; D008745:Methylation; D008840:Microfilament Proteins; D009363:Neoplasm Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D021122:Protein Subunits / Q000235:genetics; D012313:RNA / Q000378:metabolism; D014018:Tissue Distribution; D060167:Uroplakin Ia
D023181:Antimicrobial Cationic Peptides; C451410:FXYD5 protein, human; C578702:HAMP protein, human; D064451:Hepcidins; D007473:Ion Channels; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D021122:Protein Subunits; C086451:UPK1A protein, human; D060167:Uroplakin Ia; D012313:RNA; D003596:Cytosine; C513775:COX6B1 protein, human; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases