Evaluation and comparison of two immunoturbidimetric assays for the heterologous determination of porcine serum C-reactive protein.
The objective was first to evaluate the performance (intra- and inter-assay imprecision, inaccuracy, detection limit and overlap performance) of two different commercially available human C-reactive protein (CRP) immunoturbidimetric assays (TIA) applied to porcine serum CRP; and secondly to investigate inter-laboratory discrepancies and the influence of calibration materials when comparing results. The intra-laboratory performance seemed acceptable for clinical purposes for both assays with excellent overlap performance, acceptable detection limits, no significant inaccuracies and low imprecision. Although a significant discrepancy in the measured CRP levels was observed when comparing results, the methods agreed significantly on diagnostic classification, suggesting a low clinical significance. Both assays performed reliably for the heterologous determination of porcine serum CRP for clinical purposes. The results emphasise the need to establish species-specific standards to decrease inter-laboratory discrepancies.
Central Laboratory, Department of Small Animal Clinical Sciences, The Royal Veterinary and Agricultural University, 3 Gronnegaardsvej, DK-1870 Frederiksberg, Denmark. mjkh@kvl/dk
Mads
M
Kjelgaard-Hansen
Silvia
S
Martínez-Subiela
Henrik H
HH
Petersen
Asger L
AL
Jensen
Jose J
JJ
Ceron
D000818:Animals; D002097:C-Reactive Protein / Q000032:analysis* / Q000276:immunology; D019411:Clinical Laboratory Techniques / Q000592:standards / Q000662:veterinary*; D009391:Nephelometry and Turbidimetry / Q000379:methods / Q000592:standards / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013552:Swine / Q000097:blood*; D013553:Swine Diseases / Q000097:blood*
D002097:C-Reactive Protein