In vitro antifungal efficacy of four irrigants as a final rinse.
The purpose of this study was to compare the antifungal efficacy of 6% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate (CHX), 17% ethylene-diaminetetraacetic acid (EDTA), and BioPure MTAD as a final rinse on Candida albicans in vitro. Forty-eight, single rooted teeth were randomly divided into four groups each with a positive and a negative control. After root canal preparation, experimental teeth were inoculated with Candida albicans (ATCC 60193) and incubated for 72 hours. The groups were rinsed as follows: 1 ml of 6% NaOCl, 0.2 ml of 2% CHX, 1 ml of 17% EDTA, and 5 ml of BioPure MTAD. Aliquots from the experimental teeth were plated on Sabouraud 4% dextrose agar plates and colony-forming units were counted as a measure of antifungal activity. The results showed that 6% NaOCl and 2% CHX were equally effective and statistically significantly superior to BioPure MTAD and 17% EDTA (p < 0.05) in antifungal activity.
Naval Postgraduate Dental School, Bethesda, Maryland, USA.
Melissa L
ML
Ruff
Scott B
SB
McClanahan
Britta S
BS
Babel
D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D015169:Colony Count, Microbial; D004318:Doxycycline / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D011136:Polysorbates / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology
D000935:Antifungal Agents; C475043:MTAD (intracanal irrigant); D011136:Polysorbates; D012388:Root Canal Irrigants; D019343:Citric Acid; D004492:Edetic Acid; D012973:Sodium Hypochlorite; C010882:chlorhexidine gluconate; D004318:Doxycycline; D002710:Chlorhexidine