Leucocyte depletion improves renal function during reperfusion using an experimental isolated haemoperfused organ preservation system.
BACKGROUND
Leucocytes have been implicated as mediators of renal ischaemia-reperfusion injury. This study aimed to demonstrate the effect of white cells in early renal reperfusion injury using an isolated haemoperfused porcine kidney model.


METHODS
After 2 h cold storage, porcine kidneys were perfused with normothermic autologous blood using an isolated organ preservation system. This was designed using cardiopulmonary bypass technology, and perfusion commenced with a circulating serum creatinine level of 1000 micromol/l. In group 1 (n = 6) a leucocyte filter was included in the circuit and in group 2 (n = 6) non-filtered blood was used.


RESULTS
The mean(s.d.) area under the curve for serum creatinine was lower in the leucocyte-depleted experiments (1286(214) versus 2627(418); P = 0.002). Leucocyte depletion also led to improved urine output (191(75) versus 70(32) ml/h; P = 0.002) and higher creatinine clearance (10.6(2.8) versus 1.9(1.0) ml/min; P = 0.002). Renal blood flow, oxygen consumption and acid-base homeostasis were all improved by perfusion with leucocyte-depleted blood, and histological tubular damage was ameliorated.


CONCLUSIONS
These data show that the depletion of leucocytes from blood used to perfuse porcine kidneys improved postschaemic renal function, indicating that white cells play an important role in renal ischaemia-reperfusion injury.
Department of Cardiovascular Sciences, Transplant Group, Leicester General Hospital, Leicester LE5 4PW, UK.
S
S
Harper
S
S
Hosgood
M
M
Kay
M
M
Nicholson
D000136:Acid-Base Equilibrium; D000818:Animals; D003404:Creatinine / Q000097:blood; D006439:Hemodynamics / Q000502:physiology; D006464:Hemoperfusion / Q000379:methods*; D007668:Kidney / Q000098:blood supply* / Q000502:physiology; D047589:Leukocyte Reduction Procedures; D009926:Organ Preservation; D010101:Oxygen Consumption; D015427:Reperfusion Injury / Q000097:blood / Q000503:physiopathology / Q000517:prevention & control*; D013552:Swine
D003404:Creatinine