Effect of cryohypobiosis on conservation of human blood leukocytes.
Morphological and functional indicators were investigated of donor leukocyte concentrates mixed at a ratio of 1 to 1 with three versions of cold-protective and kept at an undercooling temperature (-10 degree C) for 24 hours, and with a cryoprotector of optimal composition for 12 days,. An optimal version of a non-freezing cold-protective solution was found. It was determined that 76+/-4 % of the neutrophils retain there ability of phagocytosis among the viable cells (81+/-5 %) after a 12 day hypobiosis. Thus the results testify to a high morphofunctional safety of human blood leukocytes which experienced cold-hypobiosis (-10 degree C) in an original non-freezing solution not requiring washing after re-warming.
Blood Cryophysiology Laboratory of the Physiology Institute at the Scientific Center affiliated to the Ural branch of the Russian Academy of Sciences, Komi Republic, Syktyvkar, Russia.
E P
EP
Svedentsov
E S
ES
Stepanova
T V
TV
Tumanova
O O
OO
Shcheglova
O N
ON
Devetyarova
S V
SV
Utyomov
D015925:Cryopreservation; D003451:Cryoprotective Agents; D004801:Eosine Yellowish-(YS); D005456:Fluorescent Dyes; D006801:Humans; D007962:Leukocytes / Q000166:cytology* / Q000502:physiology; D009504:Neutrophils / Q000502:physiology; D010587:Phagocytosis
D003451:Cryoprotective Agents; D005456:Fluorescent Dyes; D004801:Eosine Yellowish-(YS)