Lipids and DNA oxidation in Staphylococcus aureus as a consequence of oxidative stress generated by ciprofloxacin.
Ciprofloxacin induced an increment of reactive oxygen species in sensitive strains of Staphylococcus aureus leading to oxidative stress detected by chemiluminescence while resistant strains did not suffer such stress. Oxidation of lipids was performed by employing thiobarbituric acid reaction to detect the formation of the amplified intermediate between reactive species oxygen and cytoplasmic macromolecules, namely malondialdehyde (MDA). The sensitive strain presented higher peroxidation of lipids than the resistant strain. The oxidative consequence for DNA was investigated by means of bacteria incubation with ciprofloxacin and posterior extraction of DNA, which was studied by high performance liquid chromatography (HPLC). Sensitive S. aureus ATCC 29213 showed an increase of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) respect controls without antibiotic; there was evident increase of the ratio between 8-oxodG and deoxyguanosine (dG) as a consequence of oxidation of dG to 8-oxodG considered the major DNA marker of oxidative stress. The resistant strain showed low oxidation of DNA and the analysis of 8-oxodG/dG ratio indicated lesser formation of 8-oxodG than S. aureus ATCC 29213.
Departamento Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, Ciudad Universitaria, 5000, Córdoba, Argentina.
María Cecilia
MC
Becerra
Paulina Laura
PL
Páez
Laura E
LE
Laróvere
Inés
I
Albesa
D000890:Anti-Infective Agents / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D004269:DNA, Bacterial / Q000187:drug effects* / Q000378:metabolism; D004351:Drug Resistance; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008163:Luminescent Measurements; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism
D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D002939:Ciprofloxacin