Development of a rapid extraction procedure for speciation of arsenic in chicken meat.
A rapid extraction procedure has been developed for speciation of arsenic in chicken tissue. Water, methanol-water (1:1), and methanol-chloroform (1:1) were tested as extraction media. Individual use of an ultrasonic bath, a microwave oven, or an ultrasonic probe was not sufficient for quantitative recovery of As(III), dimethylarsinate, monomethylarsonate, As(V), and arsenobetaine in spiked samples of chicken tissue. A new extraction procedure using a methanol-water mixture and a microwave oven then an ultrasonic probe enabled extraction of the arsenic species in 7 min with efficiencies ranging from 80 to 100%. HPLC-UV-HG-AFS was used for the determinations. The extraction procedure was 100% efficient when applied to real samples of chicken tissue. AsB (48+/-5 microg As kg (-1)) and one containing-arsenic feed additive, Nitarsone (227+/-5 microg As kg (-1)) were detected.
Department of Chemistry and Materials Science, Faculty of Experimental Sciences, University of Huelva, 21071, Huelva, Spain. rodas@uhu.es
Daniel
D
Sánchez-Rodas
José
J
Luis Gómez-Ariza
Vanesa
V
Oliveira
D000818:Animals; D001151:Arsenic / Q000302:isolation & purification*; D001152:Arsenicals / Q000032:analysis / Q000302:isolation & purification*; D002645:Chickens; D008460:Meat / Q000592:standards*; D000432:Methanol; D008872:Microwaves; D014867:Water
D001152:Arsenicals; D014867:Water; C015389:nitarsone; D001151:Arsenic; D000432:Methanol