[Determination of triterpene delta-amyrone in Sedum sarmentosum].
OBJECTIVE
To establish the quantitative analysis method of triterpene delta-amyrone in Sedum sarmentosum by HPLC-ELSD.


METHODS
The Zorbax Sil Column was used and the mobile phase was dichloromethane-methanol (99:1). The flow rate was set at 0.5 mL x min(-1), the temperature of drift tube was set at 100 degrees C and the nebulizer nitrogen flow rate was 3.0 L x min(-1).


RESULTS
The linear range of sigma-amyrone was 2.12-10.6 microg. The average recovery was 100.5%.


CONCLUSIONS
The results showed that the method was reliable, simple and precise. It would be beneficial for the study of the quality control of S. sarmentosum.
Cao
Su-ping
SP
Department of Chinese Pharmceutical Analysis, China Pharmaceutical University, Nanjing.
Liu
Li-fang
LF
Wang
Qiang
Q
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D031963:Sedum / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis*
D014315:Triterpenes; C511312:amyrone