Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family.
Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporins that enable fast and controlled translocation of water across the membrane. In this study, we systematically identified and cloned ten PIP genes from rice. Based on the similarity of the amino acid sequences they encoded, these rice PIP genes were classified into two groups and designated as OsPIP1-1 to OsPIP1-3 and OsPIP2-1 to OsPIP2-7 following the nomenclature of PIP genes in maize. Quantitative RT-PCR analysis identified three root-specific and one leaf-specific OsPIP genes. Furthermore, the expression profile of each OsPIP gene in response to salt, drought and ABA treatment was examined in detail. Analysis on transgenic plants over-expressing of either OsPIP1 (OsPIP1-1) or OsPIP2 (OsPIP2-2) in wild-type Arabidopsis, showed enhanced tolerance to salt (100 mM of NaCl) and drought (200 mM of mannitol), but not to salt treatment of higher concentration (150 mM of NaCl). Taken together, these data suggest a distinct role of each OsPIP gene in response to different stresses, and should add a new layer to the understanding of the physiological function of rice PIP genes.
Guo
Lei
L
Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, National Laboratory for Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
Wang
Zi Yi
ZY
Lin
Hong
H
Cui
Wei Er
WE
Chen
Jun
J
Liu
Meihua
M
Chen
Zhang Liang
ZL
Qu
Li Jia
LJ
Gu
Hongya
H
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D020346:Aquaporins / Q000235:genetics*; D017360:Arabidopsis / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics*; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000494:pharmacology
D020346:Aquaporins; D010940:Plant Proteins; C110065:major intrinsic protein, plant; D008353:Mannitol; D012965:Sodium Chloride; D000040:Abscisic Acid