Brd4 is involved in multiple processes of the bovine papillomavirus type 1 life cycle.
Brd4 protein has been proposed to act as a cellular receptor for the bovine papillomavirus type 1 (BPV1) E2 protein in the E2-mediated chromosome attachment and mitotic segregation of viral genomes. Here, we provide data that show the involvement of Brd4 in multiple early functions of the BPV1 life cycle, suggest a Brd4-dependent mechanism for E2-dependent transcription activation, and indicate the role of Brd4 in papillomavirus and polyomavirus replication as well as cell-specific utilization of Brd4-linked features in BPV1 DNA replication. Our data also show the potential therapeutic value of the disruption of the E2-Brd4 interaction for the development of antiviral drugs.
Institute of Technology, University of Tartu, Tartu 50411, Estonia.
Ivar
I
Ilves
Kristina
K
Mäemets
Toomas
T
Silla
Kadri
K
Janikson
Mart
M
Ustav
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000145:classification / Q000502:physiology*; D016466:CHO Cells; D002417:Cattle; D018797:Cell Cycle Proteins; D002460:Cell Line; D006224:Cricetinae; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D018274:Electroporation; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology; D005822:Genetic Vectors; D009687:Nuclear Proteins; D015514:Oncogene Proteins, Fusion / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids; D017434:Protein Structure, Tertiary; D014157:Transcription Factors; D015533:Transcriptional Activation; D014779:Virus Replication; D000092468:Bromodomain Containing Proteins
D018797:Cell Cycle Proteins; D004279:DNA, Viral; D009687:Nuclear Proteins; D015514:Oncogene Proteins, Fusion; D014157:Transcription Factors; C441289:BRD4 protein, human; D000092468:Bromodomain Containing Proteins