[Studies on Ru(bipy) 2dppx(2+) as interacting mode spectroscopic probe of DNA].
The interacting mode of adriamycin with DNA has been studied by the use of molecular "light switch" complex Ru(bipy)2-dppx(2+) as a interacting mode spectroscopic probe. The results of the influence of ADM on the fluorescence and Scatchard equation of Ru(bipy)2 dppx(2+) -DNA system indicate that ADM intercalates the base pairs of DNA. Compared with normal DNA interacting mode spectroscopic probe EB, Ru(bipy)2dppx(2+) has the advantages of higher sensitivity, lower toxicity, higher stability and simplicity.
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Fan
F
Chen
Zhi-Ke
ZK
He
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis*; D015336:Molecular Probe Techniques; D009942:Organometallic Compounds / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D009942:Organometallic Compounds; D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA