[Transcriptional regulation of dentin sialophosphoprotein by c-Jun/c-Fos].
OBJECTIVE
To investigate the role of c-Jun and c-Fos as transcriptional factors in regulation of dentin sialophosphoprotein (DSPP) gene by a promoter-luciferase reporter gene construct in odontoblast cell line MDPC-23.


METHODS
Endogenous c-Jun or c-Fos protein was determined by immunocytochemistry. The role of c-Jun or c-Fos in transcription of DSPP was investigated in co-transfection experiments using promoter-luciferase reporter gene construct containing the sequence between -791 bp and +54 bp of mouse DSPP gene.


RESULTS
c-Jun and c-Fos was expressed by MDPC-23 cells, and located in the nucleus of MDPC-23 cells. Overexpression of c-Jun or c-Fos significantly inhibited luciferase activity of DSPP promoter.


CONCLUSIONS
These findings suggest c-Jun and c-Fos down-regulated the transcription of DSPP gene as a transcriptional factor in odontoblast.
Dept. of Conservative Dentistry, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Wen-Xi
WX
He
Zhong-Ying
ZY
Niu
Shou-Liang
SL
Zhao
Ping
P
Li
Jie
J
Gao
D000818:Animals; D002460:Cell Line; D016326:Extracellular Matrix Proteins; D005786:Gene Expression Regulation; D051379:Mice; D009804:Odontoblasts; D010750:Phosphoproteins; D011401:Promoter Regions, Genetic; D012795:Sialoglycoproteins; D014162:Transfection; D000099311:Dentin Sialophosphoprotein
D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein