Soluble triggering receptor expressed on myeloid cells (sTREM-1): a new mediator involved in the pathogenesis of peptic ulcer disease.
OBJECTIVE
Triggering receptor expressed on myeloid cells (TREM-1) is a promoter of cytokine production triggered by microbial components. To investigate the significance of its soluble counterpart, sTREM-1, for the pathogenesis of peptic ulcer disease, sTREM-1 was compared with the proinflammatory mediators and the pathology score of gastritis.


METHODS
Forty patients with dyspepsia were enrolled: 20 with peptic ulcer and 20 controls without any macroscopic abnormalities. All patients were examined by endoscopy; gastric juice was aspirated and biopsy specimens were collected from the antrum and corpus of the stomach. sTREM-1 was estimated by a hand-made enzyme immunoassay. Interleukin-8 was estimated by enzyme-linked immunosorbent assay and lipid peroxidation, indexed by malondialdehyde, by the thiobarbituric assay, after passage through a high-performance liquid chromatography system.


RESULTS
The median (+/-SE) of sTREM-1 of controls and patients with ulcer was 3.91+/-0.57 and 44.27+/-241.55 RU, respectively (P=0.006). The median (+/-SE) of interleukin-8 of controls and patients with ulcer was 1802.97+/-122.10 and 2030.66+/-64.44 pg/ml, respectively (P=0.023). sTREM-1 was positively correlated with the density of neutrophil and mononuclear infiltration scores and the total Sydney score (P=0.029, 0.043 and 0.041, respectively). sTREM-1 was positively correlated with interleukin-8 (P=0.042).


CONCLUSIONS
sTREM-1 might be an independent factor involving with the peptic ulcerative inflammatory process that is positively correlated with histopathological abnormalities of gastritis.
Koussoulas
Vassilios
V
Department of Gastroenterology, Sismanoglion General Hospital of Athens, Greece. basilkoussoulas3@hotmail.com
Vassiliou
Spyridon
S
Demonakou
Maria
M
Tassias
Georgios
G
Giamarellos-Bourboulis
Evangelos J
EJ
Mouktaroudi
Maria
M
Giamarellou
Helen
H
Barbatzas
Charalambos
C
D016207:Cytokines / Q000097:blood; D004381:Duodenal Ulcer / Q000097:blood / Q000503:physiopathology*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000097:blood; D007413:Intestinal Mucosa / Q000503:physiopathology; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008562:Membrane Glycoproteins / Q000097:blood / Q000378:metabolism / Q000502:physiology; D008875:Middle Aged; D022423:Myeloid Cells / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000097:blood / Q000378:metabolism / Q000502:physiology; D018709:Statistics, Nonparametric; D013276:Stomach Ulcer / Q000097:blood / Q000503:physiopathology*; D000073871:Triggering Receptor Expressed on Myeloid Cells-1
D016207:Cytokines; D016209:Interleukin-8; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D008315:Malondialdehyde