[Effect of disc displacement on mRNA expression of urokinase plasminogen activator and its inhibitor-1 in synovial tissues].
OBJECTIVE
To investigate the effect of anterior disc displacement on the expression of urokinase plasminogen activator and its inhibitor-1 (uPA/PAI-1) in synovial tissues.


METHODS
Forty Japanese white rabbits were used in this study. The animals were killed at 4 days, 1, 2, 4, 8 and 12 weeks postoperatively, respectively. In situ hybridization technology was applied to detect the expression of uPA/PAI-1 mRNA in synovial membrane.


RESULTS
In normal synovial tissues, synovial lining cells and a few fibrosblasts with mild positive staining were occasionally seen. More synovial lining cells and fibrosblasts with moderate postive signals were found 1 week after operation. Since then, the degree of staining for uPA/PAI-1 increased gradually. By the end of 12 weeks postoperatively, strong signals of uPA/PAI-1 mRNA were detected.


CONCLUSIONS
There is a harmonized uPA/PAI-1 system existing in synovial tissues. The high expression of uPA and PAI-1 mRNA in synovial tissues indicates that the uPA/PAI-1 system may play an important role in the process of synovitis resulted from anterior disc displacement.
Dept. of Oral and Maxillofacial Surgery, Hospital of Stomatology, Zhejiang University, Hangzhou 310006, China.
Jing
J
Zhan
Li-Qun
LQ
Wu
Zhi-Yuan
ZY
Gu
Yin-Kai
YK
Zhang
Ji-An
JA
Hu
D000818:Animals; D017403:In Situ Hybridization; D010958:Plasminogen; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D011817:Rabbits; D013583:Synovial Membrane; D014568:Urokinase-Type Plasminogen Activator
D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D010958:Plasminogen; D014568:Urokinase-Type Plasminogen Activator