Investigation of optical coherence tomography as an imaging modality in tissue engineering.
Monitoring cell profiles in 3D porous scaffolds presents a major challenge in tissue engineering. In this study, we investigate optical coherence tomography (OCT) as an imaging modality to monitor non-invasively both structures and cells in engineered tissue constructs. We employ time-domain OCT to visualize macro-structural morphology, and whole-field optical coherence microscopy to delineate the morphology of cells and constructs in a developing in vitro engineered bone tissue. The results show great potential for the use of OCT in non-invasive monitoring of cellular activities in 3D developing engineered tissues.
Institute of Science and Technology in Medicine, Keele University, Staffs ST4 7QB, UK.
Ying
Y
Yang
Arnaud
A
Dubois
Xiang-pei
XP
Qin
Jian
J
Li
Alicia
A
El Haj
Ruikang K
RK
Wang
D000293:Adolescent; D018929:Cell Culture Techniques; D002460:Cell Line; D006801:Humans; D019344:Lactic Acid; D008297:Male; D010011:Osteocytes / Q000166:cytology*; D011091:Polyesters; D011108:Polymers; D023822:Tissue Engineering; D041623:Tomography, Optical Coherence / Q000379:methods*
D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)