[Construction of hPARP1-deficient cell strain by RNA interference].
OBJECTIVE
To construct hPARP1-deficient cell strain which can be used in studying the functions and action mechanisms of hPARP1 gene.


METHODS
16-HBEs were transfected with two kinds of eukaryotic expression plasmids of hPARP1 gene short hairpin RNA to construct hPARP1-deficient cells (named as "16-HBEP1 and 16-HBEP2") and a negative control plasmids (named as 16-HBEN). The protein expression levels of hPARP1 gene in 16-HBE, 16-HBEP1 16-HBEP2 and 16-HBEN were detected by the western blotting to estimate the effects of RNA interference.


RESULTS
The construction of hPARP1-deficient cell strain was successful and the protein expression level of hPARP1 gene in the 16-HBEP1 and 16-HBEP2 were decreased 84.3% and 63.7% respectively as compared with that in 16-HBE.


CONCLUSIONS
The successes in constructing hPARP1-deficient strain offered an effective cell strain for studying the function of hPARP1 gene.
Sha
Yan
Y
Faculty of Preventive Medicine, School of Public Health, Sun Yet-san University, Guangzhou 510089, China.
Zeng
Yu-yun
YY
Zhuang
Zhi-xiong
ZX
He
Yun
Y
D001980:Bronchi / Q000166:cytology*; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000096:biosynthesis / Q000172:deficiency* / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
D034741:RNA, Small Interfering; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases