Stable RNA interference in Spodoptera frugiperda cells by a DNA vector-based method.
Double-stranded RNA (dsRNA)-mediated interference (RNAi) is a powerful tool for silencing of gene expression in many organisms. To establish a DNA vector-based method for stable RNAi in Spodoptera frugiperda cells (Sf9), we created a stably transfected Sf9 cell line to express large dsRNA fragment targeting to silence the firefly luciferase gene (luc). The luc dsRNA specifically and stably suppressed the baculovirus-mediated luciferase expression. Thus, gene silencing in Sf9 cells was achieved using DNA vectors similar to the facile design described in this study.
Department of Life Science and Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC.
Chih-Chien
CC
Lin
John T-A
JT
Hsu
Kai-Ling
KL
Huang
Hung-Kuan
HK
Tang
Yiu-Kay
YK
Lai
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D004247:DNA / Q000008:administration & dosage / Q000235:genetics*; D020868:Gene Silencing / Q000502:physiology; D005822:Genetic Vectors / Q000235:genetics; D007313:Insecta; D049409:Luciferases, Firefly / Q000502:physiology*; D034622:RNA Interference / Q000502:physiology*; D018411:Spodoptera / Q000502:physiology*; D014162:Transfection / Q000379:methods*
D004247:DNA; D049409:Luciferases, Firefly