Decreased ANT content in Zucker fatty rats: relevance for altered hepatic mitochondrial bioenergetics in steatosis.
Mitochondria from Zucker fatty (ZF) rats (a model for fatty liver disease) showed a delay in the repolarization after a phosphorylative cycle and a decrease on state 3 respiration, suggesting alterations at the phosphorylative system level. The ATPase activity showed no differences between control and ZF rats, implying alterations in other components of the phosphorylative system. A pronounced depletion in the content of the adenine nucleotide translocator (ANT) was observed by Western blotting, while no alterations were found in the mitochondrial voltage-dependent anion channel content. These data suggest that hepatic accumulation of fat impairs mitochondrial function, reflecting the loss of oxidative phosphorylation capacity caused by a decrease in the ANT content.
Teodoro
João
J
Center for Neurosciences and Cell Biology, Department of Zoology, University of Coimbra, 3004-517 Coimbra, Portugal.
Rolo
Anabela P
AP
Oliveira
Paulo J
PJ
Palmeira
Carlos M
CM
D033741:Adenine Nucleotide Translocator 1 / Q000378:metabolism*; D000818:Animals; D004734:Energy Metabolism; D005234:Fatty Liver / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000166:cytology / Q000473:pathology; D008297:Male; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D011924:Rats, Zucker; D050994:Voltage-Dependent Anion Channels / Q000378:metabolism
D033741:Adenine Nucleotide Translocator 1; D050994:Voltage-Dependent Anion Channels