Inhibition of the Synthesis of Wax D Peptidoglycolipid of Mycobacterium tuberculosis by d-Cycloserine.
The antibiotic d-cycloserine inhibits the synthesis of the wax D peptidoglycolipid in cells of the H37Ra strain of Mycobacterium tuberculosis. This inhibition occurs at a lower concentration of the drug than that required for the inhibition of growth. The inhibition is completely reversed by d-alanine. An arabino-galactan-galactosamine-(diaminopimelic acid)-mycolate accumulates in H37Ra cells exposed to the antibiotic. The chemical and functional group analyses of this lipid suggests it to be a possible precursor to the synthesis of wax D.
Tuberculosis Research Laboratory, Veterans Administration Hospital, and the Institute for Enzyme Research, University of Wisconsin, Madison, Wisconsin 53705.
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David
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Goldman
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Takayama