Semi-quantitative immunohistochemical analysis of male rat-specific alpha2u-globulin accumulation for chemical toxicity evaluation.
We purified male rat urinary alpha(2u)-globulin, prepared the antibody in rabbits, and improved an immunohistochemical detection method using this antibody for male rat-specific alpha(2u)-globulin accumulation appearing as hyaline droplets in the kidneys. Our prepared antibody reacted specifically with alpha(2u)-globulin in both immunohistochemical and Western blotting analyses, furthermore, and the graded immuno-reactivities on the slide were well associated with computational image analyzing results. Using this method, we retrospectively analyzed the renal sections from the toxicity studies of 12 nephrotoxic chemicals, which had already been conducted under the Japanese Existing Chemicals Survey Program. We demonstrated that the hyaline droplets induced by treatment with 10 chemicals (1,4-dibromobenzene, dicyclopentadiene, 3,4-dimethylaniline, 1,4-dicyanobenzene, tetrahydrothiophene-1,1-dioxide, 1,3-dicyanobenzene, acenaphthene, 3,4-dichloro-1-butene, 3a,4,7,7a-tetrahydro-1H-indene and 3,5,5-trimethylhexan-1-ol) were directly associated with alpha(2u)-globulin accumulation. This immunohistochemical method is convenient for applying, even retrospectively, paraffin sections from general toxicity studies and could be useful for qualifying male rat-specific hyaline droplets consisting of alpha(2u)-globulin and renal risk in humans.
Hamamura
Masao
M
Panapharm Laboratories Co., Ltd.,1285 Kurisaki-machi, Uto-shi, Kumamoto 869-0425, Japan. hamamura@panapharm.co.jp
Hirose
Akihiko
A
Kamata
Eiichi
E
Katoku
Koshiro
K
Kuwasaki
Emiko
E
Oshikata
Takafumi
T
Nakahara
Yutaka
Y
Ema
Makoto
M
Hasegawa
Ryuichi
R
D000510:Alpha-Globulins / Q000276:immunology* / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D053138:Cyclohexenes; D005260:Female; D006818:Hyalin / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D000077222:Limonene; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009930:Organic Chemicals / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013729:Terpenes / Q000494:pharmacology; D018675:Toxicity Tests / Q000379:methods*
D000510:Alpha-Globulins; D000906:Antibodies; D053138:Cyclohexenes; D009930:Organic Chemicals; D011084:Polycyclic Aromatic Hydrocarbons; D013729:Terpenes; C013803:alpha 2u globulin; D000077222:Limonene