Evaluation of 5 new media containing extracts of seeds applied to Candida dubliniensis screening.
Candida dubliniensis is a recently described pathogenic species that shares many phenotypic features with Candida albicans and so may be misidentified in microbiologic laboratories. The aim of this study is to find a useful and cost-effective method suitable for screening C. dubliniensis before proceeding to further identification. We examined the colony morphology and chlamydospore production of 26 C. dubliniensis isolates and 100 C. albicans isolates on the following 5 proposed media: sesame seed agar (SSA), rapeseed agar, canary grass seed agar, millet seed agar, and linseed agar (LA). The best results were obtained with SSA and LA because all 26 C. dubliniensis isolates showed rough colonies with peripheral hyphal fringes and abundant chlamydospores after 24 to 48 h of incubation at 25 degrees C. All C. albicans isolates (100%) showed smooth colonies without hyphal fringes or chlamydospores. These 2 media consist of new and simple tools for presumptive differentiation of C. dubliniensis from C. albicans.
Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, Santa Maria (RS), 97010-033, Brazil.
Erico Silva
ES
Loreto
Andreza Ribeiro
AR
Bolzan
Carlos Eduardo Blanco
CE
Linares
Everton
E
Boff
Janio Moraes
JM
Santurio
Sydney Hartz
SH
Alves
D002175:Candida / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D002176:Candida albicans / Q000145:classification / Q000502:physiology; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods; D013172:Spores, Fungal / Q000145:classification
D003470:Culture Media