Neuronal activity determines the protein synthesis dependence of long-term potentiation.
Long-term potentiation (LTP) is generally divided into two phases, early (E-) and late (L-) LTP, of which only L-LTP is thought to depend on protein synthesis. Here we report that E-LTP can also be dependent on protein synthesis at higher levels of synaptic activation. Moreover, we show that the requirement for protein synthesis during L-LTP extends beyond the early induction phase and that it depends on synaptic stimulation. This suggests that the level of neuronal activity is a crucial determinant for the role of protein synthesis in E- and L-LTP.
Department of Cellular and Systems Neurobiology, Max Planck Institute of Neurobiology, Am Klopferspitz 18, D-82152 München-Martinsried, Germany.
Rosalina
R
Fonseca
U Valentin
UV
Nägerl
Tobias
T
Bonhoeffer
D000818:Animals; D000841:Anisomycin / Q000378:metabolism; D004594:Electrophysiology; D017774:Long-Term Potentiation / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D013569:Synapses / Q000378:metabolism*; D009435:Synaptic Transmission
D011500:Protein Synthesis Inhibitors; D016194:Receptors, N-Methyl-D-Aspartate; D000841:Anisomycin