Effects of amino acid on morphological development and nucleus formation of arachidonic acid-producing filamentous micro-organism, Mortierella alpina.
OBJECTIVE
Effects of amino acid on morphological development and nucleus formation of arachidonic acid-producing filamentous micro-organism, Mortierella alpina were investigated using flow-through chamber.


RESULTS
Mortierella alpina CBS 754.68 was cultivated in flow through chamber using nutrient-rich, minimal and specific amino acid-containing minimal media. To investigate the effect of amino acid on morphological parameters either 0.28 g l(-1) alanine, 0.53 g l(-1) sodium glutamate one hydrate or 0.42 g l(-1) valine was added to the minimal medium. In a flow-through chamber, the growth of hyphal elements and nucleus formation of arachidonic acid-producing fungus M. alpina were studied on-line, using image analysis techniques. When the Ala- and Val-containing media were used, the hyphal growth units (HGUs) were 90.2 and 86.7 microm per tip, respectively, which were 2.4-fold higher than that in the nutrient-rich medium, indicating that Ala and Val stimulate the elongation of hyphae. The specific nucleus formation rates were Glu->Val-containing media>minimal and nutrient-rich media>Ala-containing medium. The nucleus doubling times in Glu- and Val-containing media were 1.9 and 2 h, respectively, which were not significant different.


CONCLUSIONS
Ala and Val stimulate the elongation of M. alpina hyphae, and nucleus formation rates were Glu->Val->Ala-containing media.


CONCLUSIONS
Formation of fungal morphology and nucleus were shown using the flow-through chamber coupled with image analysis, which making possible to discuss the relationship between mycelial morphology and nucleus formation of M. alpina.
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan.
K
K
Koizumi
K
K
Higashiyama
E Y
EY
Park
D000409:Alanine / Q000378:metabolism / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000096:biosynthesis*; D002467:Cell Nucleus / Q000187:drug effects*; D003470:Culture Media; D018698:Glutamic Acid / Q000378:metabolism; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D020094:Mortierella / Q000187:drug effects / Q000254:growth & development*; D012970:Sodium Glutamate / Q000378:metabolism / Q000494:pharmacology; D014633:Valine / Q000378:metabolism / Q000494:pharmacology
D000596:Amino Acids; D003470:Culture Media; D016718:Arachidonic Acid; D018698:Glutamic Acid; D014633:Valine; D000409:Alanine; D012970:Sodium Glutamate