Comparison of carbon dioxide, argon, and nitrogen for inducing unconsciousness or euthanasia of rats.
We compared CO(2), Ar, and N(2) for inducing unconsciousness and euthanasia of Sprague-Dawley rats. We determined time to unconsciousness and monitored heart rate (HR) and mean arterial blood pressure (MAP) by radiotelemetry to assess stress, recovery after exposure, and time of death. Unconsciousness (mean +/- standard error) occurred 24 +/- 3, 87 +/- 8, and 93 +/- 8 s after short-term exposure to CO(2), Ar, and N(2), respectively. During exposure, CO(2) depressed HR, whereas Ar and N(2) increased HR. Upon removal from the chamber, rats' HR rapidly normalized after CO(2) or N(2) but remained elevated for 60 min after Ar. During exposure, all agents depressed MAP, which returned to resting levels 10 to 50 min after rats' removal from the chamber. For euthanasia, CO(2) at approximately 100% induced unconsciousness in 37 +/- 3 s, increased and then depressed MAP and HR, and caused death at 188 +/- 15 s. CO(2) at approximately 30% induced unconsciousness in 150 +/- 15 s, decreased HR and MAP, and induced death at 440 +/- 9 s. Ar at approximately 100% increased MAP but decreased HR, induced unconsciousness with hyperreflexia at 54 +/- 4 s, and caused death at 197 +/- 20 s. N(2) at approximately 100% decreased MAP but not HR and produced unconsciousness with hyperreflexia at 164 +/- 17 s and death at 426 +/- 28 s. We conclude that CO(2) effectively produced unconsciousness and euthanasia, but we were unable to ascertain distress. Ar also appears effective but produced hyperreflexia and tachycardia. N(2) was ineffective.
Sharp
Jody
J
Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA.
Azar
Toni
T
Lawson
David
D
D000818:Animals; D000830:Animals, Laboratory; D001128:Argon / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D002245:Carbon Dioxide / Q000494:pharmacology*; D037901:Euthanasia, Animal / Q000379:methods*; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D008297:Male; D009584:Nitrogen / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012021:Reflex, Abnormal / Q000187:drug effects; D013997:Time Factors; D014474:Unconsciousness / Q000139:chemically induced* / Q000503:physiopathology
D002245:Carbon Dioxide; D001128:Argon; D009584:Nitrogen