[Influence of high molecular weight polyethylene on viability of osteoblasts and new bone formation].
To investigate the influence of high molecular weight polyethylene (HMWP) on the viability of osteoblasts and new bone formation in the process of fracture healing, the osteoblasts derived from adult human bone marrow were cultured in HMWP maceration extract and normal culture medium. The viability of the osteoblasts was measured by MTT assay, and the function of the osteoblasts was detected by use of alkaline phosphatase test kit. The locked double-plating (steel plate and HMWP plate) was implanted and fixed at the artificial fracture of distal femur of dogs. Specimens were gained at 3, 6, 9 and 12 weeks postoperatively, examined with macroscopy, microscope and scanning electron microscope (SEM). The results showed that HMWP did no harm to osteoblasts. There is no significant difference in activities of proliferation and alkaline phosphatase between HMWP maceration extract and normal culture medium at each observation time of at 2,4,8, and 14 dyas (P>0. 05). Bone tissue under the implanted HMWP plate manifested no absorption; the new bones formed under the HMWP plate and gradually matured as time went on. It is demonstrated in this study that HMWP has no adverse influence on the viability of osteoblasts and new bone formation and it can be used as internal fixation implant in treating fractures.
(Department of Orthopaedic Traumatology, Nanfang Hospital, Nanfang Medical University, Guangzhou, China. rgaoh@163.com
Gaohong
G
Ren
Angru
A
Lin
Guoxian
G
Pei
Basheng
B
Hu
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D005264:Femoral Fractures / Q000601:surgery; D005593:Fracture Fixation, Internal; D017102:Fracture Healing / Q000502:physiology; D006801:Humans; D020007:Implants, Experimental; D016268:Internal Fixators; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D020959:Polyethylene / Q000737:chemistry / Q000494:pharmacology*
D001672:Biocompatible Materials; D020959:Polyethylene