Colorimetric detection of immunoglobulin G by use of functionalized gold nanoparticles on polyethylenimine film.
A method based on use of functionalized gold nanoparticles on polyethylenimine film has been developed for colorimetric detection of immunoglobulin G (IgG). The immunogold nanoparticles were immobilized on quartz slides by recognition between antibody and antigen, with the antigen chemically adsorbed on the polyethylenimine film. By measurement of the UV-visible spectra of the immobilized immunogold, detection of h-IgG was achieved. The detection limit for h-IgG by use of this method can be as low as 0.01 microg mL(-1). This method is quite promising for numerous applications in immunoassay.
Li
Haiqiu
H
Chemistry Department, Northeast Normal University, Changchun, 130024, P. R. China.
Wang
Chungang
C
Ma
Zhanfang
Z
Su
Zhongmin
Z
D015374:Biosensing Techniques; D003102:Colloids / Q000737:chemistry; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D011094:Polyethyleneimine / Q000032:analysis*; D011791:Quartz; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
D003102:Colloids; D007074:Immunoglobulin G; D011791:Quartz; D006046:Gold; D011094:Polyethyleneimine