Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium.
pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient. Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.
Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan. mita2001@sfc.keio.ac.jp
Mitsuhiro
M
Itaya
Nagayoshi
N
Sakaya
Satoko
S
Matsunaga
Kyoko
K
Fujita
Shinya
S
Kaneko
D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D003227:Conjugation, Genetic / Q000187:drug effects* / Q000502:physiology*; D003470:Culture Media / Q000494:pharmacology*; D007700:Kinetics; D010957:Plasmids / Q000187:drug effects / Q000235:genetics*
D003470:Culture Media