Cryopreservation of Quercus robur L. embryogenic calli.
Embryogenic calli of pedunculate oak (Quercus robur L.) were cryopreserved using direct immersion in liquid nitrogen. The pretreatment consisted of culture on a solid medium with increasing sucrose concentrations (0.25 M for 1 day, 0.5 M for 1 day, 0.75 M for 2 days, and 1.0 M for 3 days), followed by air desiccation of embryogenic calli to 17.3 percent (fresh weight basis). This method of cryoprotection was compared to a liquid cryoprotection treatment using high concentrations of sucrose solutions, followed by glycerol solutions. Regrowth of frozen tissue pretreated on a solid medium was significantly higher than those pretreated in the liquid solutions.
Polish Academy of Sciences, Institute of Dendrology, 62-035 Kornik, Parkowa 5, Poland. pach@rose.man.poznan.pl.
Pawel
P
Chmielarz
Ghislaine
G
Grenier-de March
Marie-Thérèse
MT
Boucaud
D002460:Cell Line; D002478:Cells, Cultured; D015925:Cryopreservation; D003451:Cryoprotective Agents; D003470:Culture Media; D003890:Desiccation; D029963:Quercus; D012639:Seeds / Q000166:cytology* / Q000254:growth & development; D013395:Sucrose
D003451:Cryoprotective Agents; D003470:Culture Media; D013395:Sucrose