Detection of Bacillus thuringiensis kurstaki HD1 on cabbage for human consumption.
The objectives of the study were to develop a specific procedure for quantification and identification of Bacillus thuringiensis kurstaki HD1, which is used as a biopesticide, and to quantify its presence in different kinds of cabbage for human consumption. We found that B. thuringiensis kurstaki HD1 can be distinguished from other B. thuringiensis strains by its unique random amplification of polymophic DNA-PCR pattern with the OPA9 primer and the presence of the flagellin genes, as detected by the primers FLAB1 and FLAB2. We detected from one to 100 Bacillus cereus-like bacteria in 10 batches of five different cabbage products for consumption. As many as 73 out of 134 isolates (53.7%) were identical with B. thuringiensis kurstaki HD1. The results show that B. thuringiensis kurstaki HD1 from biopesticides can be found in vegetables for human consumption.
Department of Environmental Chemistry and Microbiology, National Environmental Research Institute, Frederiksborgvej, Roskilde, Denmark. nbh@dmu.dk
Niels Bohse
NB
Hendriksen
Bjarne Munk
BM
Hansen
D001409:Bacillus cereus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001413:Bacillus thuringiensis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001937:Brassica / Q000382:microbiology*; D005408:Flagellin / Q000235:genetics*; D005516:Food Microbiology; D006801:Humans; D010572:Pest Control, Biological; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique
D005408:Flagellin