A Method for Purification of Clostridium perfringens Phospholipase C from Recombinant Bacillus subtilis Cells.
We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.
Hirata
Y
Y
Minami
J
J
Koyama
M
M
Matsushita
O
O
Katayama
S
S
Jin
F
F
Maeta
H
H
Okabe
A
A