Nitric oxide and polyamine pathway-dependent modulation of neutrophil free amino- and alpha-keto acid profiles or host defense capability.
We have examined the effects of N(omega)-nitro-L-arginine-methylester-hydrochloride [L-NAME; inhibitor of nitric oxide synthase], S-nitroso-N-acetyl-penicillamine [SNAP; nitric oxide donor], alpha-difluoro-methyl-ornithine [DFMO; inhibitor of ornithine decarboxylase] arginine or ornithine as well as the combination of arginine or ornithine with L-NAME, SNAP or DFMO on intracellular free amino- and alpha-keto acid profiles and the immune function markers superoxide anion and hydrogen peroxide generation as well as released myeloperoxidase activity in neutrophils (PMN). Although the underlying mechanisms still remain unclear, we believe from our results that nitric oxide as well as polyamine-dependent pathways are involved in the signal transmission of free radical molecule, beneficial nutritional therapy or maleficient pharmacological stress-induced alterations in PMN nutrient composition. Relevant changes in intragranulocyte free amino- and alpha-keto acid homeostasis and metabolism, especially, may be one of the determinants in PMN nutrition that positively or negatively influences and modulate neutrophil host defence capability and immunocompetence.
Mühling
J
J
Department of Anaesthesiology, Intensive Care Medicine, Pain Therapy, University Hospital Giessen and Marburg, Giessen, Federal Republic of Germany. joerg.muehling@chiru.med.uni-giessen.de
Engel
J
J
Halabi
M
M
Müller
M
M
Fuchs
M
M
Krüll
M
M
Harbach
H
H
Langefeld
T W
TW
Wolff
M
M
Matejec
R
R
Welters
I D
ID
Menges
T
T
Hempelmann
G
G
D000328:Adult; D000596:Amino Acids / Q000378:metabolism* / Q000494:pharmacology; D000518:Eflornithine / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007651:Keto Acids / Q000378:metabolism*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D010396:Penicillamine / Q000031:analogs & derivatives / Q000494:pharmacology; D009195:Peroxidase / Q000187:drug effects; D011073:Polyamines / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
D000596:Amino Acids; D007651:Keto Acids; D011073:Polyamines; C110881:S-nitro-N-acetylpenicillamine; D013481:Superoxides; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D009195:Peroxidase; D010396:Penicillamine; D019331:NG-Nitroarginine Methyl Ester; D000518:Eflornithine