Solid-phase extraction of 4(5)-methylimidazole (4MeI) and 2-acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)-imidazole (THI) from foods and beverages with subsequent liquid chromatographic-electrospray mass spectrometric quantification.
A method for the simultaneous determination of 4(5)-methylimidazole (4MeI) and 2-acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)-imidazole (THI) was developed using SPE and HPLC/MS. Solid-phase extraction using SCX Disc cartridges was used for isolation of the analytes from liquid samples. The lower LOQwas 0.1 ng/mL for 4MeI and 0.2 ng/ mL for THI. The linearity of the calibration curves was satisfactory as indicated by correlation coefficients >0.999. The CV for the intra- and inter-day precision was <5% (n = 6); the accuracy was in the range 98-103%. The recovery was > or = 97 and > or = 98% for THI and 4MeI, respectively. The method was used to determine THI and 4MeI in beverages, coffee, caramel colours and other samples.
Klejdus
Borivoj
B
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry Brno, Czech Republic.
Moravcová
Jitka
J
Lojková
Lea
L
Vacek
Jan
J
Kubán
Vlastimil
V
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis; D007093:Imidazoles / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization
D007093:Imidazoles; C049929:2-acetyl-4(5)-tetrahydroxybutylimidazole; C023439:4-methylimidazole