Tetraploid and hexaploid wheats express identical isoforms of nsLTP1.
Nonspecific lipid-transfer proteins (nsLTPs) have been recognized as allergens in several plant species among which are cereals important in human nutrition. In this report, we purified a 9600 +/- 1 Da protein from both soft wheat and farro bran. Mass spectrometric analyses revealed that these proteins are identical, belong to the nsLTP1 class, and have high sequence homology with nsLTP1 isolated from other cereal species. Their identification was further supported by the ability of the soft wheat nsLTP1 to transfer pyrene-labeled lipids between donor and acceptor membranes. The results are discussed in view of the increasing diffusion on the markets of bran-rich products.
Capocchi
Antonella
A
Department of Botanical Sciences, University of Pisa, via L. Ghini 5, 56126 Pisa, Italy. capocchi@dsb.unipi.it
Fontanini
Debora
D
Muccilli
Vera
V
Cunsolo
Vincenzo
V
Saviozzi
Franco
F
Saletti
Rosaria
R
Foti
Salvatore
S
Galleschi
Luciano
L
D002352:Carrier Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D050356:Lipid Metabolism; D010743:Phospholipids / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis*; D020033:Protein Isoforms / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D014908:Triticum / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism
D002352:Carrier Proteins; D010743:Phospholipids; D010940:Plant Proteins; D020033:Protein Isoforms; D014357:Trypsin