Close relationship between SARS-coronavirus and group 2 coronavirus.
The sudden appearance and potential lethality of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) in humans has resulted in a focusing of new attention on the determination of both its origins and evolution. The relationship existing between SARS-CoV and other groups of coronaviruses was determined via analyses of phylogenetic trees and comparative genomic analyses of the coronavirus genes: polymerase (Orf1ab), spike (S), envelope (E), membrane (M) and nucleocapsid (N). Although the coronaviruses are traditionally classed into 3 groups, with SARS-CoV forming a 4th group, the phylogenetic position and origins of SARS-CoV remain a matter of some controversy. Thus, we conducted extensive phylogenetic analyses of the genes common to all coronavirus groups, using the Neighbor-joining, Maximum-likelihood, and Bayesian methods. Our data evidenced largely identical topology for all of the obtained phylogenetic trees, thus supporting the hypothesis that the relationship existing between SARS-CoV and group 2 coronavirus is a monophyletic one. Additional comparative genomic studies, including sequence similarity and protein secondary structure analyses, suggested that SARS-CoV may bear a closer relationship with group 2 than with the other coronavirus groups. Although our data strongly suggest that group 2 coronaviruses are most closely related with SARS-CoV, further and more detailed analyses may provide us with an increased amount of information regarding the origins and evolution of the coronaviruses, most notably SARS-CoV.
Division of Life Sciences and Research Institute for Biotechnology, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Ok Ju
OJ
Kim
Dong Hun
DH
Lee
Chan Hee
CH
Lee
D017934:Coronavirus / Q000145:classification* / Q000235:genetics*; D019143:Evolution, Molecular; D016679:Genome, Viral; D023281:Genomics; D006801:Humans; D010802:Phylogeny; D017433:Protein Structure, Secondary; D045473:Severe acute respiratory syndrome-related coronavirus / Q000145:classification* / Q000235:genetics*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D014764:Viral Proteins