Prevention of lethal hepatic injury in Long-Evans Cinnamon (LEC) rats by D-galactosamine hydrochloride.
Repeated injections of D-galactosamine hydrochloride (GalN) increase the survival rate of Long-Evans Cinnamon (LEC) rats, an animal model of Wilson's disease. The aim of the present study was to investigate the mechanism of GalN for prevention of spontaneous lethal hepatic injury in LEC rats. Male LEC rats were given a single subcutaneous injection of 300 mg/kg of GalN or vehicle (0.9% NaCl) at 14 weeks, and killed at 28 weeks of age. Next, 6-week-old male LEC rats were given weekly subcutaneous injections of 300 mg/kg of GalN or vehicle for 3 or 12 weeks, and their hepatic 8-hydroxydeoxy-2'-guanosine (8-OHdG), glutathione peroxidase (GPX), and catalase activities were measured. None of GalN-treated rats died of hepatic injury (0/12), whereas the mortality rate of control rats given 0.9% NaCl was 17% (2/12). GalN administration for 12 weeks decreased the hepatic 8-OHdG, and GalN administration for either 3 or 12 weeks increased the glutathione peroxidase activity. GalN administration increased the serum level of alanine aminotransferase, and accelerated megalocytic degeneration of the hepatocytes. GalN treatment is effective in preventing lethal hepatitis in LEC rats and decrease of oxidative DNA damage by GalN plays an important role in increase of the survival rate.
Otsuka
Toshihiro
T
Department of Digestive and Pediatric Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan.
Izumi
Keisuke
K
Tokunaga
Itsuo
I
Gotohda
Takako
T
Ipposhi
Kaneshige
K
Takiguchi
Yoshiharu
Y
Kaneda
Shinya
S
Satake
Nobuo
N
Ohnishi
Takamasa
T
Tashiro
Seiki
S
Shimada
Mitsuo
M
D000818:Animals; D004195:Disease Models, Animal; D005609:Free Radicals / Q000378:metabolism; D005688:Galactosamine / Q000494:pharmacology*; D006527:Hepatolenticular Degeneration / Q000188:drug therapy / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051381:Rats; D020332:Rats, Inbred LEC
D005609:Free Radicals; D005688:Galactosamine