[Effects of different manipulation methods of acupuncture at Zusanli (ST 36) on signal transduction pathway of STAT5 in human PBMC].
OBJECTIVE
To study on effects of different manipulation methods of acupuncture on the binding ability of signal transducers and activators of transcription (STAT5) in human peripheral blood mononuclear cells (PBMC) with DNA.


METHODS
Thirty healthy volunteers were randomly divided into 3 groups; reinforcing method group, reducing method group, normal control group, 10 cases in each group. The expression of STAT5 mRNA and the activation of STAT5 in the human PBMC were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and electrophoretic mobility shift assay (EMSA).


RESULTS
In the reinforcing method group, the basic transcription level of STATS mRNA in human PBMC and the binding ability of STAT5 with DNA significantly increased (P<0.01), but in the reducing method group, they did not significantly change as compared with those in the normal control group (P>0.05).


CONCLUSIONS
Cytokines and JAK/STAT signal transduction pathway are in volved in immunoregulative actions of acupuncture of the reinforcing method, but the reasons of influencing the transcription level of STAT5 mRNA and the binding ability of STAT5 with DNA are unclear.
Liu
Zhi-bin
ZB
Affiliated Hospital of Shaanxi College of TCM, Xianyang 712020, China. lzb210396@163.com
Yang
Xiao-hang
XH
D015670:Acupuncture Therapy; D006801:Humans; D007963:Leukocytes, Mononuclear; D012333:RNA, Messenger; D050799:STAT5 Transcription Factor; D015398:Signal Transduction
D012333:RNA, Messenger; D050799:STAT5 Transcription Factor