Posttranslational regulation of Mycobacterium tuberculosis extracytoplasmic-function sigma factor sigma L and roles in virulence and in global regulation of gene expression.
In this report, we demonstrate that SigL is posttranslationally regulated by a specific anti-sigma factor, RslA, and contributes to the expression of at least 28 genes. Several of these genes could mediate important cell envelope-related processes. Importantly, a sigL-rslA mutant strain was significantly attenuated in a mouse model of infection.
Dainese
Elisa
E
Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Via Gabelli 63, 35100 Padova, Italy.
Rodrigue
Sébastien
S
Delogu
Giovanni
G
Provvedi
Roberta
R
Laflamme
Liette
L
Brzezinski
Ryszard
R
Fadda
Giovanni
G
Smith
Issar
I
Gaudreau
Luc
L
Palù
Giorgio
G
Manganelli
Riccardo
R
D000818:Animals; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000472:pathogenicity; D011499:Protein Processing, Post-Translational / Q000276:immunology*; D012808:Sigma Factor / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D014397:Tuberculosis, Pulmonary / Q000382:microbiology / Q000401:mortality; D037521:Virulence Factors / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology
D012808:Sigma Factor; D037521:Virulence Factors