Effect of formalin treatment on electrophoretic mobility of cholera toxin.
Crude cholera toxin detoxified by treatment with Formalin showed an increased mobility and a decrease in the number of detectable protein constituents when compared with the parent toxin by disc electrophoresis. The significance of these observations in relation to the enhanced antigenic activity of cholera toxoid as compared to parent toxin is discussed.
Division of Biologics Standards, National Institutes of Health, Bethesda, Maryland 20014.
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Esposito
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Feeley