Down-regulation of hepatocyte growth factor mRNA in rat cardiac myocytes under hypoxia mimicked by cobalt chloride.
Hypoxia is generally considered to represent a fundamental stimulus for angiogenesis. Most angiogenic factors were up-regulated by hypoxia, but as a strong angiogenic and antiapoptotic factor, hepatocyte growth factor (HGF) was down-regulated by hypoxia. In order to investigate whether hypoxia inducible factor 1a (HIF-1alpha) participate in the transcriptional regulation of HGF under hypoxia, rat cardiac myocytes were treated with cobalt chloride (CoCl2), the specific inducer of HIF-1alpha, for different times, and total RNA and protein were isolated to perform RT-PCR and Western blot. Results show the expression of HGF mRNA in cardiac myocytes decreased distinctively after treating with CoCl2 for 12 hours. However, at the same time, the expression of HIF-1alpha protein was up-regulated. HIF-1alpha may be participate in the transcriptional regulation of HGF indirectly.
Institute of Microcirculation, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.
Yan
Y
Su
Hongwei
H
Li
Jing
J
Zhang
Ruijuan
R
Xiu
D000818:Animals; D002478:Cells, Cultured; D003035:Cobalt; D004195:Disease Models, Animal; D015536:Down-Regulation; D017228:Hepatocyte Growth Factor / Q000235:genetics*; D000860:Hypoxia / Q000139:chemically induced / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D003035:Cobalt; D017228:Hepatocyte Growth Factor; C018021:cobaltous chloride