Polyhemoglobin with different percentage of tetrameric hemoglobin and effects on vasoactivity and electrocardiogram.
There has been considerable discussions on why some types of haemoglobin-based blood substitutes increase vasoactivity whereas a very few others do not. In this study, we prepare four different types of PolyHb each containing different percentage of tetrameric hemoglobin using glutaraldehyde crosslinking and characterized to ensure that they all have the same oxygen affinity. Thus the preparations are prepared from the same chemical method and have the same oxygen affinity. We infused these in the form of 1/6 volume toploading into anesthetized rats to simulate the use of blood substitutes in surgery. Mean arterial pressure (MAP) increased immediately after injection of PolyHb containing 38% or 78% of tetrameric hemoglobin. However, there was no significant increase in blood pressure with the injection of PolyHb containing 16% or 0.4% tetrameric hemoglobin. In electrocardiogram (ECG) study, we observe that high percentage (78%) of tetrameric hemoglobin causes marked changes in ECG immediately after infusion. Injection of PolyHb containing 16% or 38% of tetrameric hemoglobin resulted in minimal elevation of the ST segment. Infusion of PolyHb containing 0.4% of tetrameric hemoglobin did not result in any changes.
Artificial Cell and Organ Research Center, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
Binglan
B
Yu
Zunchang
Z
Liu
Thomas Ming Swi
TM
Chang
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D001802:Blood Substitutes / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D004562:Electrocardiography; D006454:Hemoglobins / Q000008:administration & dosage* / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D007262:Infusions, Intravenous; D008297:Male; D008970:Molecular Weight; D010100:Oxygen / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D001802:Blood Substitutes; D006454:Hemoglobins; C037158:polyhemoglobin; D010100:Oxygen