Large-scale neural ensemble recording in the brains of freely behaving mice.
With the availability of sophisticated genetic techniques, the mouse is a valuable mammalian model to study the molecular and cellular basis of cognitive behaviors. However, the small size of mice makes it difficult for a systematic investigation of activity patterns of neural networks in vivo. Here we report the development and construction of a high-density ensemble recording array with up to 128-recording channels that can be formatted as single electrodes, stereotrodes, or tetrodes. This high-density recording array is capable of recording from hundreds of individual neurons simultaneously in the hippocampus of the freely behaving mice. This large-scale in vivo ensemble recording techniques, once coupled with mouse genetics, should be valuable to the study of complex relationship between the genes, neural network, and cognitive behaviors.
Lin
Longnian
L
Shanghai Institute of Brain Functional Genomics, The Key laboratories of MOE and SSTC, East China Normal University, Shanghai 200062, China.
Chen
Guifen
G
Xie
Kun
K
Zaia
Kimberly A
KA
Zhang
Shuqing
S
Tsien
Joe Z
JZ
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D004567:Electrodes, Implanted / Q000592:standards; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D006624:Hippocampus / Q000502:physiology*; D051379:Mice; D008839:Microelectrodes / Q000592:standards; D009068:Movement / Q000502:physiology; D009415:Nerve Net / Q000502:physiology; D009434:Neural Pathways / Q000502:physiology; D009482:Neurophysiology / Q000295:instrumentation* / Q000379:methods; D017966:Pyramidal Cells / Q000502:physiology*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation