A functional proteomics approach for the detection of nuclear proteins based on derepressed importin alpha.
The identification of functional proteomes is a major challenge in proteomic research. Here we describe a method for the detection and isolation of nuclear (localization sequence containing) proteins using a derepressed import receptor (DIRE) as a synthetic antibody. We demonstrate that the DIRE method specifically detects nuclear localization sequence containing proteins. Application to activation of primary T-lymphocytes exemplifies the potential use of DIRE for comparative proteomics and for diagnostics.
Blazek
Erik
E
Department of Gene Expression, National Research Center for Environment and Health-GSF, Institute of Molecular Immunology, Munich, Germany.
Meisterernst
Michael
M
D000595:Amino Acid Sequence; D026481:Blotting, Far-Western; D002467:Cell Nucleus / Q000737:chemistry*; D002478:Cells, Cultured; D006801:Humans; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D017384:Sequence Deletion; D028901:alpha Karyopherins / Q000032:analysis* / Q000235:genetics* / Q000502:physiology
D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D028901:alpha Karyopherins