In vitro binding interactions of oral bacteria with immobilized fructosyltransferase.
OBJECTIVE
The objective of the present study was to explore the role of immobilized fructosyltransferase (FTF) in adhesion process.


RESULTS
We investigated real-time biospecific interactions between several types of oral bacteria and recombinant FTF immobilized on a biosensor chip, using surface plasmon resonance technology. Streptococcus mutans, Streptococcus sobrinus and Actinomyces viscosus demonstrated significant binding to FTF. Actinomyces viscosus had a greater binding to FTF, with 373 Resonance Units (RU), than the other tested bacteria. The binding level to FTF of Strep. sobrinus was 320 RU, whereas Strep. mutans and Streptococcus salivarious show binding of 296 and 245 RU, respectively. The binding sensograms displayed different profiles for the tested bacteria at various cell density, suggesting a different affinity to immobilized FTF.


CONCLUSIONS
The results from this study suggest that FTF may influence bacterial adherence and colonization of the dental biofilm.


CONCLUSIONS
The biomolecular interaction analysis enables real-time monitoring of the interaction between adhesions of intact bacteria and their ligands, which might be crucial in the initial phase of biofilm development in vivo.
Shemesh
M
M
Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, Jerusalem, Israel.
Steinberg
D
D
D016924:Actinomyces viscosus / Q000502:physiology*; D001422:Bacterial Adhesion / Q000502:physiology; D015374:Biosensing Techniques; D015169:Colony Count, Microbial / Q000379:methods; D006602:Hexosyltransferases / Q000378:metabolism / Q000502:physiology*; D009055:Mouth / Q000382:microbiology*; D013291:Streptococcus / Q000502:physiology*; D013295:Streptococcus mutans / Q000502:physiology; D016986:Streptococcus sobrinus / Q000502:physiology; D020349:Surface Plasmon Resonance / Q000379:methods; D001426:Bacterial Proteins
D006602:Hexosyltransferases; C021451:inulosucrase; D001426:Bacterial Proteins