Oral immunization of mice with plant-derived fimbrial adhesin FaeG induces systemic and mucosal K88ad enterotoxigenic Escherichia coli-specific immune responses.
The importance of adhesins in pathogenicity has resulted in them being useful targets in the defense against bacterial infections. To produce edible vaccines against piglet diarrhea caused by enterotoxigenic Escherichia coli (ETEC), plants were genetically engineered to produce recombinant fimbrial adhesin FaeG. To evaluate the efficacy of the edible vaccine FaeG in mice, the soluble protein extracts were examined by about 15 microg recombinant FaeG for each oral immunization dose per mouse. After four doses of vaccination, both IgG and IgA antibodies specific to K88ad fimbriae were elicited in serum, and specific IgA antibodies were also evoked in feces of the immunized mice. Moreover, visible K88ad ETEC agglutination by the specific serum from the immunized mice was observed, implying the antibody was highly specific and effective. Results from an in vitro villous-adhesion assay further confirmed that serum antibodies of the immunized mice could inhibit K88ad ETEC from adhering to pig intestinal receptors, further demonstrating the oral immune efficacy of the plant-derived FaeG. This study provides a promising, noninvasive method for vaccinating swine by feeding supplements of transgenic plant. Moreover, the low cost and ease of delivery of this edible ETEC vaccine will facilitate its application in economically disadvantaged regions.
Liang
Wanqi
W
School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Huang
Yahong
Y
Yang
Xinghong
X
Zhou
Zhiai
Z
Pan
Aihu
A
Qian
Bingjun
B
Huang
Cheng
C
Chen
Jianxiu
J
Zhang
Dabing
D
D018830:Adhesins, Escherichia coli / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D000284:Administration, Oral; D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001422:Bacterial Adhesion / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D007114:Immunization / Q000662:veterinary; D007421:Intestine, Small / Q000276:immunology / Q000382:microbiology; D051379:Mice; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
D018830:Adhesins, Escherichia coli; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; C494388:FaeG protein, E coli; D011994:Recombinant Proteins