The Tre2 oncoprotein, implicated in Ewing's sarcoma, interacts with two components of the cytoskeleton.
The product of the Tre2 oncogene, structurally related to the Ypt/RabGTPase-activating proteins (Ypt/RabGAP), is involved in various human cancers, including Ewing's sarcoma. In order to identify proteins interacting with the GAP part of this protein, we performed yeast two-hybrid screening of two libraries. Two components of the cytoskeleton were thus identified, whose interaction with the GAP region was confirmed by GST-pulldown, co-immunoprecipitation, and colocalisation experiments. The proteins found to interact with the GAP region are the light regulatory chain of myosin II (Myl2) and LOC91256, a protein containing ankyrin repeats.
Animal and Microbial Biology Unit, Gembloux Agricultural University, Avenue Maréchal Juin 6, Gembloux, B-5030, Belgium.
Christophe
C
Dechamps
Stephane
S
Bach
Daniel
D
Portetelle
Micheline
M
Vandenbol
D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D024462:Myosin Type II / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011518:Proto-Oncogene Proteins; D012512:Sarcoma, Ewing / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D043222:Ubiquitin Thiolesterase
D003598:Cytoskeletal Proteins; D009363:Neoplasm Proteins; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D010450:Endopeptidases; C473330:USP6 protein, human; D043222:Ubiquitin Thiolesterase; D024462:Myosin Type II