Physiological response to superantigen-adsorbing hemoperfusion in toxin-concentration-controlled septic swine.
OBJECTIVE
Superantigens are suspected of being potent initiators of gram-positive sepsis, and new therapies for superantigen elimination are required. The effects of hemoadsorption with a superantigen-adsorbing device (SAAD) were evaluated in septic swine.


METHODS
Toxic shock syndrome toxin-1 (TSST-1) was infused, and blood concentration was maintained at the clinical level for 6 h. Endotoxin was then infused to induce lethal shock. All animals were hemoperfused with SAAD or a control column for 8 h and changes in pathological parameters and mortality were examined.


RESULTS
Animals perfused with SAAD had a highly significant (p < 0.01) survival advantage compared with control groups at 24 h after initiation of the TSST-1 infusion. SAAD also suppressed the increase in the arteriovenous shunt ratio and decrease of partial arterial oxygen pressure at 6 h after TSST-1 infusion initiation.


CONCLUSIONS
We suggest that there is a potential application of SAAD in treating superantigen-induced respiratory dysfunction and sepsis.
Miwa
Keishi
K
New Frontiers Research Laboratories, Toray Industries, Inc., Kanagawa, Japan. Keishi_Miwa@nts.toray.co.jp
Fukuyama
Mayumi
M
Matsuno
Naoto
N
Shimada
Kaoru
K
Ikeda
Kazumi
K
Ikeda
Toshiaki
T
D000327:Adsorption; D000818:Animals; D001427:Bacterial Toxins / Q000097:blood* / Q000633:toxicity; D002318:Cardiovascular Diseases / Q000503:physiopathology / Q000628:therapy; D016207:Cytokines / Q000097:blood / Q000502:physiology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000097:blood; D004768:Enterotoxins / Q000097:blood* / Q000633:toxicity; D006464:Hemoperfusion; D008297:Male; D012120:Respiration Disorders / Q000503:physiopathology / Q000628:therapy; D018805:Sepsis / Q000139:chemically induced / Q000503:physiopathology* / Q000628:therapy*; D018089:Superantigens / Q000097:blood* / Q000633:toxicity; D015996:Survival Rate; D013552:Swine; D013997:Time Factors
D001427:Bacterial Toxins; D016207:Cytokines; D004731:Endotoxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal