Strain improvement of Candida tropicalis for the production of xylitol: biochemical and physiological characterization of wild-type and mutant strain CT-OMV5.
Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81 g of xylitol per gram of xylose. This was further mutated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85 g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea. The CT-OMV5 mutant strain differs by 12 tests when compared to the wild-type Candida tropicalis strain. The XR activity was higher in mutant CT-OMV5. The distinct difference between the mutant and wild-type strain is the presence of numerous chlamydospores in the mutant. In this investigation, we have demonstrated that mutagenesis was successful in generating a superior xylitol-producing strain, CT-OMV5, and uncovered distinctive biochemical and physiological characteristics of the wild-type and mutant strain, CT-OMV5.
Rao
Ravella Sreenivas
RS
Department of Microbiology, Omania University, Hyderabad-500 007, India.
Jyothi
Cherukuri Pavana
CP
Prakasham
Reddy Shetty
RS
Rao
Chaganti Subba
CS
Sarma
Ponnupalli Nageshwara
PN
Rao
Linga Venkateswar
LV
D001709:Biotechnology / Q000379:methods; D041022:Candida tropicalis / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D016296:Mutagenesis; D009154:Mutation; D014466:Ultraviolet Rays; D014993:Xylitol / Q000096:biosynthesis*; D014994:Xylose / Q000378:metabolism
D003470:Culture Media; D008769:Methylnitronitrosoguanidine; D014994:Xylose; D014993:Xylitol