PlyPH, a bacteriolytic enzyme with a broad pH range of activity and lytic action against Bacillus anthracis.
We have cloned a lytic enzyme, PlyPH, with a specific lytic effect on Bacillus anthracis strains. PlyPH remains active between pH 4 and 10.5, and a single dose rescued a significant percentage of mice infected intraperitoneally with an attenuated B. anthracis strain. We propose PlyPH as a novel therapeutic agent.
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Pauline
P
Yoong
Raymond
R
Schuch
Daniel
D
Nelson
Vincent A
VA
Fischetti
D000595:Amino Acid Sequence; D000818:Animals; D000881:Anthrax; D001408:Bacillus anthracis / Q000145:classification / Q000378:metabolism* / Q000502:physiology; D001433:Bacteriolysis; D001435:Bacteriophages / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D013379:Substrate Specificity; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
D014764:Viral Proteins