The origin of spontaneous synchronized burst in cultured neuronal networks based on multi-electrode arrays.
Many neural networks in mammalian central nervous system (CNS) fire single spike and complex spike burst. In fact, the conditions for triggering burst are not well understood. In the paper multi-electrode arrays (MEA) are used to record the spontaneous electrophysiological activities of cultured rat hippocampal neuronal network for a long time. After about 3 weeks culture, a transition from single spike to burst is observed in several networks. All of these spikes fire quickly before burst begins. The firing rate during the burst is lower than that just before the burst, but differences of inter-spike intervals (ISIs) between two firing patterns are not clear. Moreover, the electrical activities on neighboring electrodes show strong synchrony during the burst activities. In a word, the generation of the burst requires that network should have a sufficient level of excitation as well as a balance of synaptic inhibition.
Chen
Chuanping
C
Key Laboratory of Biomedical Photonics, Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China. chchuanp2002@etang.com
Chen
Lin
L
Lin
Yunsheng
Y
Zeng
Shaoqun
S
Luo
Qingming
Q
D000818:Animals; D004566:Electrodes; D004594:Electrophysiology; D009415:Nerve Net / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013379:Substrate Specificity; D046509:Tissue Culture Techniques