Expression profiles and single-nucleotide polymorphism analysis of human HANP1/H1T2 encoding a histone H1-like protein.
Recently we cloned the Hanp1 cDNA that encodes a histone H1-like haploid germ cell-specific nuclear protein in the mouse. Homozygous Hanp1 mutant male mice were infertile, while females were fertile. Although a substantial number of sperm were recovered from the epididymis, their shape and function were abnormal. Hanp1 protein is essential for nuclear formation in functional spermatozoa, and is specifically involved in the replacement of histones with protamines during spermiogenesis. To investigate the roles of human HANP1 (h-HANP1) and its relation to male infertility, we isolated h-HANP1 cDNA from a human cDNA plasmid library using mouse Hanp1 cDNA as a probe. h-HANP1 is expressed in the testes and its genomic construct also intronless as mouse Hanp1. We found that the h-HANP1 coding region have 5 single-nucleotide polymorphisms in Japanese men.
Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan. tanaka@biken.osaka-u.ac.jp
Hiromitsu
H
Tanaka
Yasuhiro
Y
Matsuoka
Masayoshi
M
Onishi
Kouichi
K
Kitamura
Yasushi
Y
Miyagawa
Hiromi
H
Nishimura
Akira
A
Tsujimura
Akihiko
A
Okuyama
Yoshitake
Y
Nishimune
D000595:Amino Acid Sequence; D001483:Base Sequence; D016022:Case-Control Studies; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D015870:Gene Expression; D006657:Histones / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000378:metabolism; D017385:Sequence Homology; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014162:Transfection
C512208:H1-7 protein, human; D006657:Histones; D012333:RNA, Messenger