Lipid dependence of the channel properties of a colicin E1-lipid toroidal pore.
Colicin E1 belongs to a group of bacteriocins whose cytotoxicity toward Escherichia coli is exerted through formation of ion channels that depolarize the cytoplasmic membrane. The lipid dependence of colicin single-channel conductance demonstrated intimate involvement of lipid in the structure of this channel. The colicin formed "small" conductance 60-picosiemens (pS) channels, with properties similar to those previously characterized, in 1,2-dieicosenoyl-sn-glycero-3-phosphocholine (C20) or thinner membranes, whereas it formed a novel "large" conductance 600-pS state in thicker 1,2-dierucoyl-sn-glycero-3-phosphocholine (C22) bilayers. Both channel states were anion-selective and voltage-gated and displayed a requirement for acidic pH. Lipids having negative spontaneous curvature inhibited the formation of both channels but increased the ratio of open 600 pS to 60 pS conductance states. Different diameters of small and large channels, 12 and 16 A, were determined from the dependence of single-channel conductance on the size of nonelectrolyte solute probes. Colicin-induced lipid "flip-flop" and the decrease in anion selectivity of the channel in the presence of negatively charged lipids implied a significant contribution of lipid to the structure of the channel, most readily described as toroidal organization of lipid and protein to form the channel pore.
A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119992 Moscow, Russia.
Alexander A
AA
Sobko
Elena A
EA
Kotova
Yuri N
YN
Antonenko
Stanislav D
SD
Zakharov
William A
WA
Cramer
D003087:Colicins / Q000737:chemistry*; D004553:Electric Conductivity; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins; D007473:Ion Channels; D008051:Lipid Bilayers / Q000737:chemistry; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008564:Membrane Potentials; D008954:Models, Biological; D011092:Polyethylene Glycols / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
D003087:Colicins; D029968:Escherichia coli Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; D008055:Lipids; D008081:Liposomes; D012997:Solvents; D011092:Polyethylene Glycols