The relationship between proliferating cell nuclear antigen expression and histomorphometrical alterations in cyclosporin A-induced gingival overgrowth in rats.
The aim of this study was to investigate the relationship between Proliferating Cell Nuclear Antigen (PCNA) expression and histomorphometrical alterations in cyclosporin A (CsA)-induced gingival overgrowth with or without microbial dental plaque accumulation. Forty male Wistar rats were equally divided into 4 groups; Group I (control); Group II (CsA); Group III (ligature); Group IV (ligature and CsA). After 8 weeks of experimental period, rats were subsequently decapitated and mandibular molars were dissected. Gingival overgrowth was determined by measuring depth of the gingival sulcus, then the mandible were decalcified and serial sections were obtained for histomorphometric and immunohistochemical analysis. Histomorphometric analysis included the measurement of epithelial thickness; immunohistochemical analysis included the assessment of PCNA expression in the oral and sulcular epithelium of buccal and lingual gingiva. Epithelial thickness and PCNA expression were significantly increased in buccal oral epithelium of Group II (p < 0.05) and in all regions in Group IV (p < 0.05) compared to control group. Also gingival overgrowth was more prominent in Group IV in comparison to Group II. These results indicate that CsA-induced gingival alterations are closely associated with increased epithelial proliferative activity, and dental plaque accumulation seems not to be an essential but to be an aggrevating factor for the progression of the lesion.
Faculty of Dentistry, Department of Periodontology, Ondokuzmayis University, Samsun, 55139, Turkey. ozkandt@hotmail.com
Burcu Ozkan
BO
Cetinkaya
Gokhan
G
Acikgoz
Oguz
O
Aydin
Adnan
A
Korkmaz
Gonca Cayir
GC
Keles
D000818:Animals; D049109:Cell Proliferation; D016572:Cyclosporine / Q000633:toxicity*; D004848:Epithelium / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D005881:Gingiva / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D019214:Gingival Overgrowth / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000633:toxicity*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar
D007166:Immunosuppressive Agents; D018809:Proliferating Cell Nuclear Antigen; D016572:Cyclosporine