In-vitro 1H-MRS of excised rabbit lung: evaluation of preservation method of the lung using 10% DMSO solution.
OBJECTIVE
This study was performed to investigate the lactate, creatine, and choline changes in excised rabbit lung metabolites using proton magnetic resonance spectroscopy (1H-MRS).


METHODS
Rabbit lung was preserved with -20 degrees C 10% dimethyl sulfoxide (DMSO) solution until used, and the lactate, creatine, and choline levels of the rabbit lung were evaluated by 1H-MRS (6.34T superconducting unit). The control of lung tissue was excised under low-temperature conditions of liquid nitrogen, and preserved in 10% DMSO solution at -20 degrees C until evaluated. The lung tissues were excised at room temperature, and 30 min later, the tissues were saved in 10% DMSO solution at -20 degrees C until evaluated, and the lactate, creatine, and choline were measured by 1H-MRS after 20, 40, 60 and 80 days.


RESULTS
The lactate, creatine, and choline in the control tissue measured 0.412 micromol/g, 0.024 micromol/g and 0.696 micromol/g, respectively. The lactate, creatine, and choline in the experimental tissue measured 0.414 micromol/g, 0.029 micromol/g and 0.388 micromol/g after 60 days, respectively.


CONCLUSIONS
In this study, the level of lactate within tissues preserved in 10% DMSO solution at -20 degrees C achieved a state of equilibrium within 60 days.
Department of Radiology, Kanazawa Medical University, Ishikawa 920-0293, Japan.
Hajime
H
Yokota
Jianfei
J
Guo
Munetaka
M
Matoba
Kotaro
K
Higashi
Hisao
H
Tonami
Yosinobu
Y
Nagao
D000818:Animals; D002794:Choline / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D019344:Lactic Acid / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009926:Organ Preservation / Q000379:methods*; D011817:Rabbits
D019344:Lactic Acid; D003401:Creatine; D002794:Choline; D004121:Dimethyl Sulfoxide