New caerin antibiotic peptides from the skin secretion of the Dainty Green Tree Frog Litoria gracilenta. Identification using positive and negative ion electrospray mass spectrometry.
The skin secretion of the Dainty Green Tree Frog Litoria gracilenta contains 16 peptides, which protect the animal from predators, both large and small. A combination of negative and positive ion electrospray mass spectrometry together with Lys-C enzymic digest and Edman sequencing identifies three new wide-spectrum caerin 1 antibiotics, namely Caerin 1.17 [GLFSVLGSVAKHLLPHVAPIIAEKL-NH2], Caerin 1.18 [GLFSVLGSVAKHLLPHVVPVIAEKL-NH2], and Caerin 1.19 [GLFKVLGSVAKHLLPHVAPIIAEKL-NH2], and a narrow spectrum antibiotic Caerin 3.5 [GLWEKVKEKANELVSGIVEGVK-NH2].
Maclean
Micheal J
MJ
Department of Chemistry, The University of Adelaide, Adelaide, SA 5005, Australia.
Brinkworth
Craig S
CS
Bilusich
Daniel
D
Bowie
John H
JH
Doyle
Jason R
JR
Llewellyn
Lyndon E
LE
Tyler
Michael J
MJ
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry*; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D001001:Anura; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D012867:Skin / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides