Expression of pituitary adenylate cyclase-activating polypeptide 1 and 2 receptor mRNA in gallbladder tissue of patients with gallstone or gallbladder polyps.
OBJECTIVE
To detect the expression of pituitary adenylate cyclase-activating polypeptide receptor 1 (VPCAP1-R)and VPCAP2-R mRNA in gallbladder tissues of patients with gallstone or gallbladder polyps.


METHODS
The expression of VPCAP1-R and VPCAP2-R mRNA in gallbladder tissues was detected in 25 patients with gallstone,8 patients with gallbladder polyps and 7 donors of liver transplantation by reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
The VPCAP2-R mRNA expression level in the control group (1.09+/-0.58) was lower than that in the gallbladder polyp group (1.64+/-0.56) and the gallstone group (1.55+/-0.45) (P<0.05) while the VPCAP1-R mRNA expression level in the control group (1.15+/-0.23) was not apparently different from that in the gallbladder polyp group (1.28+/-0.56) and the gallstone group (1.27+/-0.38).


CONCLUSIONS
The abnormal expression of VPCAP2-R mRNA in gallbladder tissue may play a role in the formation of gallbladder stone and gallbladder polyps.
No.2 Department of General Surgery, Second Affiliated Hospital, China Medical University, Shenyang, 110004, Liaoning Province, China.
Zhen-Hai
ZH
Zhang
Shuo-Dong
SD
Wu
Hong
H
Gao
Gang
G
Shi
Jun-Zhe
JZ
Jin
Jing
J
Kong
Zhong
Z
Tian
Yang
Y
Su
D000328:Adult; D005260:Female; D005704:Gallbladder / Q000737:chemistry*; D005705:Gallbladder Diseases / Q000235:genetics* / Q000378:metabolism; D042882:Gallstones / Q000737:chemistry / Q000209:etiology / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D011127:Polyps / Q000737:chemistry / Q000209:etiology / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I / Q000235:genetics* / Q000502:physiology; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I