Destruction of Escherichia coli O157:H7 by vanillic acid in unpasteurized juice from six apple cultivars.
The behavior of Escherichia coli O157:H7 in Granny Smith, Gala, Empire, McIntosh, Red Delicious, and Golden Delicious apple juice with or without supplementation with 5 or 10 mM vanillic acid was examined over a storage period of 7 days at 4 and 15 degrees C. The consequences of supplementation on sensory difference and preference were also determined by triangle testing. Juices made from the six apple cultivars had pH values ranging between pH 3.13 and 3.92. Vanillic acid exerted a concentration, pH, and time-dependent lethal effect toward E. coli O157:H7 in unpasteurized apple juice. Supplementation with 10 mM vanillic acid led to a 5-logarithm reduction in populations after 7 days at both temperatures, but sensory analysis revealed significant differences from and preference for unsupplemented juices. Supplementation with 5 mM vanillic acid accelerated death of E. coli O157:H7, but population reductions ranged from 5 log CFU/ml in low pH juices to none in high pH juices, particularly at 4 degrees C. No sensory difference or preference was detected in two of the six juices at this level of supplementation.
Moon
Kwan Deog
KD
Department of Food Science & Technology, Kyungpook National University, 1370 Sangyeok-dong, Buk-gu, Daegu 702-701, Korea.
Delaquis
Pascal
P
Toivonen
Peter
P
Bach
Susan
S
Stanich
Kareen
K
Harris
Leanne
L
D001628:Beverages / Q000382:microbiology; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000254:growth & development; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D027845:Malus / Q000382:microbiology*; D013649:Taste; D013696:Temperature; D013997:Time Factors; D014641:Vanillic Acid / Q000494:pharmacology*
D014641:Vanillic Acid