An amperometric enzyme electrode for creatine determination prepared by the immobilization of creatinase and sarcosine oxidase in poly(vinylferrocenium).
A new enzyme electrode for the determination of creatine was developed by immobilizing creatinase (CI) and sarcosine oxidase (SO). The enzymes were co-immobilized in a poly(vinylferrocenium) matrix onto the surface of a platinum working electrode. Crosslinking with glutaraldehyte (GA) and bovine serum albumin (BSA) was selected as the best immobilization method for the enzymatic system. Determination of creatine was performed by the oxidation of enzymatically generated H2O2 at + 0.7 V vs. Ag/AgCl. The linear working range of the electrode was 2.0 x 10(-5) - 3.2 x 10(-4) M and the response time was about 50 s. The effects of pH, temperature, enzyme ratio and buffer concentration were investigated and optimum parameters were found to be 7.5, 37 degrees C, 2.5:1 (CI:SO) and 0.05 M, respectively. The stability and reproducibility of the enzyme electrode have been also studied.
Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey.
Pinar Esra
PE
Erden
Sule
S
Pekyardimci
Esma
E
Kiliç
Fatma
F
Arslan
D015374:Biosensing Techniques; D003217:Conductometry / Q000379:methods; D003401:Creatine / Q000032:analysis* / Q000097:blood; D003432:Cross-Linking Reagents / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010710:Phosphates / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry*; D050879:Sarcosine Oxidase / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013696:Temperature; D014512:Ureohydrolases / Q000737:chemistry
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D010710:Phosphates; D011145:Polyvinyls; C451099:polyvinylferrocenium; D012710:Serum Albumin, Bovine; D050879:Sarcosine Oxidase; D014512:Ureohydrolases; C019944:creatinase; D003401:Creatine; D005976:Glutaral