Probability of lethal damages of cryopreserved biological objects during storage.
This study demonstrated that with a cyclic change of the temperature from -196 degree C up to -130 degree C or -100 degree C part of cryopreserved microorganisms, tumor and human embryo cells died. This should be taken into account when moving and transporting the cryopreserved objects. Storage of samples under the above-mentioned stable temperatures did not result in additional death of cells.
Institute for problems of cryobiology and cryomedicine of the National Academy of Sciences of the Ukraine, 23 Pereyaslavskaya str., 61015, Kharkov, Ukraine. cryo@online.kharkov.ua
I P
IP
Vysekantsev
T M
TM
Gurina
V F
VF
Martsenyuk
T F
TF
Petrenko
E V
EV
Kudokotseva
S V
SV
Koshchiy
M I
MI
Groshevoy
D000818:Animals; D016923:Cell Death; D002470:Cell Survival; D015925:Cryopreservation; D006801:Humans; D008099:Liver / Q000166:cytology / Q000196:embryology; D008114:Liver Neoplasms, Experimental; D009420:Nervous System / Q000166:cytology / Q000196:embryology; D012441:Saccharomyces cerevisiae; D013211:Staphylococcus aureus; D014407:Tumor Cells, Cultured