A second-generation integrated map of the silkworm reveals synteny and conserved gene order between lepidopteran insects.
A second-generation linkage map was constructed for the silkworm, Bombyx mori, focusing on mapping Bombyx sequences appearing in public nucleotide databases and bacterial artificial chromosome (BAC) contigs. A total of 874 BAC contigs containing 5067 clones (22% of the library) were constructed by PCR-based screening with sequence-tagged sites (STSs) derived from whole-genome shotgun (WGS) sequences. A total of 523 BAC contigs, including 342 independent genes registered in public databases and 85 expressed sequence tags (ESTs), were placed onto the linkage map. We found significant synteny and conserved gene order between B. mori and a nymphalid butterfly, Heliconius melpomene, in four linkage groups (LGs), strongly suggesting that using B. mori as a reference for comparative genomics in Lepidotera is highly feasible.
Insect Genome Laboratory, National Institute of Agrobiological Sciences, Japan. yaskoch@affrc.go.jp
Yuji
Y
Yasukochi
Laksmikutty A
LA
Ashakumary
Kotaro
K
Baba
Atsuo
A
Yoshido
Ken
K
Sahara
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D017124:Conserved Sequence; D020451:Contig Mapping; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D007915:Lepidoptera / Q000166:cytology / Q000235:genetics*; D026801:Synteny
D005819:Genetic Markers