An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro.
The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5'-GCGTCGGTTTCGGTGCTCAC-3') was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-gamma (IFN-gamma) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.
Food Research and Development Laboratory, Morinaga Milk Industry Co., Ltd, Zama, Kanagawa, Japan. no_takah@morinagamilk.co.jp
Noritoshi
N
Takahashi
Haruki
H
Kitazawa
Takeshi
T
Shimosato
Noriyuki
N
Iwabuchi
Jin-Zhong
JZ
Xiao
Keiji
K
Iwatsuki
Sadayuki
S
Kokubo
Tadao
T
Saito
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D001483:Base Sequence; D001644:Bifidobacterium / Q000235:genetics* / Q000276:immunology*; D049109:Cell Proliferation; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D019936:Probiotics / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D013047:Specific Pathogen-Free Organisms
D004269:DNA, Bacterial; D007132:Immunoglobulin Isotypes; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D018664:Interleukin-12; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma