[The study on the technique of sexing bovine pre-implantation embryos with two-temperature gradient multiplex PCR].
For the stable, reliable, fast method of sexing bovine pre-implantation embryos is still play very important role in husbandry production, the amplification experiment under the condition of the two-temperature gradient PCR was done with bovine samples such as genome, cloning embryos, embryos respectively. As a result, the stable, simple, fast method of two-temperature gradient PCR for sexing bovine pre-implantation embryos was obtained, which only took 57 minutes to identify the embryo sex. A total of 30 dairy embryos were sexed with the two-temperature gradient PCR method in the study. 15 identified embryos (11 female, 4 male) were transferred into 15 recipient cows, among them 7 were pregnant after 60 days. In the end, 5 female calves were aborted in late pregnancy, and 1 female and 1 male dairy calf were born. The sexes of aborted and born calves were fully in accordance with the embryo sex predetermination with PCR method.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China. dongwang@iascaas.net.cn
Dong
D
Wang
Jin-Hua
JH
Cheng
Hua-Bin
HB
Zhu
Hai-Sheng
HS
Hao
Zong-Li
ZL
Wang
Bo
B
Yang
Zhen-Ling
ZL
Wang
Wei-Hua
WH
Du
Yong-Hua
YH
Liu
Ying-Xia
YX
Zhang
D000818:Animals; D002417:Cattle; D010064:Embryo Implantation / Q000235:genetics*; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D019849:Sex Determination Processes; D013696:Temperature