Human amniotic cell sheet harvest using a novel temperature-responsive culture surface coated with protein-based polymer.
Human amniotic epithelial (hAE) and mesenchymal (hAM) cells are believed to have the potential to differentiate into various functional cells, such as neurons, hepatocytes, cardiomyocytes, and pancreatic beta cells. However, cell transplantation has been performed by injection of cell suspensions, and thus it is difficult to control shape, size, location, and functions of differentiated cells. To overcome these problems, we developed a novel temperature-responsive culture surface coated with elastic protein-based polymer. By reducing the temperature using a polyvinylidene difluoride (PVDF) membrane, the primary hAE and hAM cell sheet can detach from the coated surface. The recovered cell sheet can be transferred and can re-adhere and re-proliferate on another surface. This represents the first report of harvesting of primary hAE and hAM cell sheets using the novel temperature- responsive polymer. These findings suggest that this new technique of cell sheet detachment from noncytotoxic, highly biocompatible protein-based polymer-coated surfaces may be useful in tissue engineering, as well as in the investigation of hAE and hAM cell sheets for transplantation.
Department of Organ Regeneration, Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
Helin
H
Zhang
Masamichi
M
Iwama
Toshihiro
T
Akaike
Dan W
DW
Urry
Asima
A
Pattanaik
Timothy M
TM
Parker
Ikuo
I
Konishi
Toshio
T
Nikaido
D000650:Amnion / Q000166:cytology*; D017209:Apoptosis; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods; D005465:Fluorocarbon Polymers
D008567:Membranes, Artificial; D011108:Polymers; D011145:Polyvinyls; D011506:Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers