Value of mink vomit model in study of anti-emetic drugs.
OBJECTIVE
To establish a new, reliable vomit model of minks.


METHODS
Adult male minks were randomly divided into 8 groups (n=6): cisplatin (7.5 mg/kg) intraperitoneal injection (i.p.) group, copper sulfate (40 mg/kg) intragastric injection (i.g.) group, apomorphine (1.6 mg/kg) subcutaneous injection (s.c.) group, and 18 Gy whole-body X-irradiation group, ondansetron injection group (2 mg/kg i.p.) 30 min later followed by cisplatin (7.5 mg/kg) i.p., normal saline (NS) i.p. injection control group, metoclopramide injection group (4 mg/kg i.p.) 30 min later followed by apomorphine (1.6 mg/kg) s.c., NS i.g. control group. The frequency of retching and vomiting was calculated. After behavioral experiment, distribution of 5-HT in the ileum was detected by immunohistologic method.


RESULTS
Cisplatin, apomorphine, copper sulfate and X-irradiation administered to minks evoked a profound emetic response in the animals. However, retching and vomiting were significantly inhibited by pretreatment with ondansetron and metoclopramide in cisplatin and copper sulfate groups (P=0.018). Immunohistologic result showed that 5-HT released from enterochromaffin cells (EC cells) was involved in vomiting mechanism.


CONCLUSIONS
Mink vomit model has a great value in studying the vomiting mechanism and screening new antiemetic drugs.
Department of Pharmacology, Medical College of Qingdao University, 38 Dengzhou Road, Qingdao 266021, Shandong Province, China. fangzhangqd@yahoo.com
Fang
F
Zhang
Lei
L
Wang
Zhi-Hong
ZH
Yang
Zhan-Tao
ZT
Liu
Wang
W
Yue
D000818:Animals; D000932:Antiemetics / Q000627:therapeutic use*; D001058:Apomorphine; D002945:Cisplatin / Q000008:administration & dosage; D019327:Copper Sulfate / Q000008:administration & dosage; D004195:Disease Models, Animal; D004759:Enterochromaffin Cells / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D008787:Metoclopramide / Q000008:administration & dosage / Q000627:therapeutic use; D008907:Mink; D017294:Ondansetron / Q000008:administration & dosage / Q000627:therapeutic use; D011897:Random Allocation; D015203:Reproducibility of Results; D012701:Serotonin / Q000378:metabolism; D014839:Vomiting / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control*
D000932:Antiemetics; D012701:Serotonin; D017294:Ondansetron; D008787:Metoclopramide; D019327:Copper Sulfate; D001058:Apomorphine; D002945:Cisplatin