Determination of the binding affinities of plasmid DNA using fluorescent intercalators possessing an acridine skeleton.
Novel acridine derivatives, wherein the steric factor has been varied systematically through substitution at the 9 position of the acridine ring, were evaluated as convenient fluorescent probes for nucleic acid detection. The binding affinities of N-(9-acridinylthiocarbamoyl)amino acids (ATA) with plasmid DNA (pUC 19) were investigated using UV-vis spectrophotometry, fluorometric titration and quantum chemical calculations (AM1). From spectrofluorometric analysis, the binding constants for the DNA-ATA complexes were determined. To elucidate its DNA intercalation, the most preferable tautomeric structure of ATA was established by means of AM1 calculations.
Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, P.J. Safárik University, 041 54 Kosice, Slovakia. sabdan@kosice.upjs.sk
D
D
Sabolová
M
M
Kozurková
P
P
Kristian
I
I
Danihel
D
D
Podhradský
J
J
Imrich
D000166:Acridines / Q000737:chemistry / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D010957:Plasmids; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000166:Acridines; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D007364:Intercalating Agents