Ultrafast enzyme immobilization over large-pore nanoscale mesoporous silica particles.
By finely tuning the TEOS/P123 molar ratio of the octane/water/P123/TEOS quadruple emulsion system and by controlling the synthesis conditions, an ultrafine emulsion system was isolated, under the confinement of which, nanoscale silica particles with ordered large mesopores (approximately 13 nm) have been successfully constructed; the obtained mesoporous silica particles have an unusual ultrafast enzyme adsorption speed and the amount of enzyme that can be immobilized is larger than that of conventional mesoporous silica, which has potential applications in the fast separation of biomolecules.
State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.
Junming
J
Sun
He
H
Zhang
Ruijun
R
Tian
Ding
D
Ma
Xinhe
X
Bao
Dang Sheng
DS
Su
Hanfa
H
Zou
D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004655:Emulsions; D004800:Enzymes, Immobilized / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors; D014961:X-Ray Diffraction
D004655:Emulsions; D004800:Enzymes, Immobilized; D012822:Silicon Dioxide