The immuno cross-reactivity of gutta percha points.
OBJECTIVE
The purpose of this study is to test the postulated immuno cross-reactivity between proteins derived from raw gutta percha (RGP), gutta percha point (GPP) and natural rubber latex (NRL).


METHODS
Antigenicity and cross-reactivity of proteins were determined by the FITkit (FITBiotech, Finland) and ELISA inhibition assays.


RESULTS
Antigenicity of proteins derived from RGP or GPP was not demonstrated. Except for NRL glove extracts, neither extracts from RGP or GPP were reactive in ELISA inhibition assay.


CONCLUSIONS
There is no immunologic cross-reactivity in vitro between proteins derived from RGP or GPP, and from NRL gloves. Thus, therapeutic use of GPP is unlikely to initiate adverse immuno-reactivity in individuals previously sensitized to NRL proteins.
Research and Laboratories, Division of Science, American Dental Association, Chicago, IL 60611, USA. kangp@ada.org
Peter B
PB
Kang
Kristy
K
Vogt
Stephen E
SE
Gruninger
Milton
M
Marshall
Chakwan
C
Siew
Daniel M
DM
Meyer
D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006171:Gutta-Percha; D007159:Immunologic Tests; D007840:Latex; D020315:Latex Hypersensitivity / Q000276:immunology*; D032130:Mimusops; D010940:Plant Proteins / Q000276:immunology*; D012387:Root Canal Filling Materials; D012408:Rubber
D007840:Latex; D010940:Plant Proteins; D012387:Root Canal Filling Materials; C439273:gutta-balata; D006171:Gutta-Percha; D012408:Rubber