Isolation and characterization of a cDNA encoding a membrane bound acyl-CoA binding protein from Agave americana L. epidermis.
A cDNA encoding an acyl-CoA binding protein (ACBP) homologue has been cloned from a cDNA library made from mRNA isolated from epidermis of young leaves of Agave americana L. The derived amino acid sequence reveals a protein corresponding to the membrane-associated form of ACBPs only previously described in Arabidopsis and rice. Northern blot analysis showed that the A. americana ACBP gene is mainly expressed in the epidermis of mature zone of the leaves. The epidermis of A. americana leaves have a well developed cuticle with the highest amounts of the cuticular components waxes, cutin and cutan suggesting a potential role of the protein in cuticle formation.
Departamento de Biología Molecular y Bioquímica, Grupo de Caracterización y Síntesis de Biopolímeros Vegetales, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain.
Consuelo
C
Guerrero
M
M
Martín-Rufián
José J
JJ
Reina
Antonio
A
Heredia
D027882:Agave / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D026261:Diazepam Binding Inhibitor / Q000378:metabolism*; D008969:Molecular Sequence Data; D019441:Plant Epidermis / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D026261:Diazepam Binding Inhibitor