Proteomic analysis of a fraction enriched in cell envelope proteins of Acinetobacter baumannii.
Acinetobacter baumannii is a multiresistant opportunistic nosocomial pathogen. A protein fraction was purified and analyzed by 2-DE. Twenty-nine major protein spots were selected for protein identification using trypsin digestion and MS analysis. As the A. baumannii genome has not yet been described, protein identification was performed by homology with other Acinetobacter species in the NCBi database. We identified ribosomal proteins, chaperones, elongation factors and outer membrane proteins (Omp), such as OmpA and the 33-36-kDa OMP. Proteomic analysis of A. baumannii provides a platform for further studies in antimicrobial resistance.
Servei de Microbiologia, Centre de Diagnòstic Biomèdic, Hospital Clínic, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain.
Sara
S
Martí
Javier
J
Sánchez-Céspedes
Eliandre
E
Oliveira
David
D
Bellido
Ernest
E
Giralt
Jordi
J
Vila
D040981:Acinetobacter baumannii / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D013347:Subcellular Fractions / Q000378:metabolism
D001426:Bacterial Proteins; D008565:Membrane Proteins; D020543:Proteome