Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice.
Seven cord blood (CB) units were tested for their capacity to repopulate irradiated NOD/SCID mice after one or two successive cryopreservation procedures. In primary transplants with frozen or refrozen CB cells we observed equivalent human colonies and percentages of human CD45+ cells, with multilineage engraftment. In secondary transplants flow cytometry and polymerase chain reaction for the a satellite region of chromosome 17 showed equivalent levels of human engraftment. Since CB units have, to date, mainly been stored in individual bags, our results suggest new options for optimizing the timing of infusions of expanded and non-expanded progenitors in transplants.
Paediatric Haematology-Oncology Department, University of Torino, Italy. fabio.timeus@unito.it
Fabio
F
Timeus
Nicoletta
N
Crescenzio
Fiorella
F
Sanavio
Luigina
L
Fazio
Alessandra
A
Doria
Luiselda
L
Foglia
Ymera
Y
Pignochino
Massimo
M
Berger
Wanda
W
Piacibello
Enrico
E
Madon
Luca
L
Cordero di Montezemolo
Franca
F
Fagioli
D000818:Animals; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D005312:Fetal Blood / Q000637:transplantation*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D013045:Species Specificity