Study on homoeologous chromosome pairing and translocation induced by 5A/5R X 6A/6R wheat-rye substitution lines.
This article aims to study the homoeologous chromosome pairing and translocation induced by 5A/5R x 6A/6R wheat-rye substitution lines. To clarify the mechanism of homoeologous chromosome pairing and create wheat-rye translocation lines, two wheat-rye substitution lines, 5A/5R and 6A/6R were crossed. The chromosome behavior of pollen mother cells (PMCs) in meiosis was investigated in hybrid F1. Homoeologous chromosome pairing between wheat and rye occurred in 22.91% of PMC. Wheat-rye translocation lines were identified via C-banding and in situ hybridization (GISH) in hybrid F2 and later generations. In F2 generation, translocations happened in 9 of 45 plants, reached to 20%. These translocation lines were generated from homoeologous chromosome pairing, or mis-division and reconstruction of univalent chromosomes.
Biology Department of Harbin Normal University, Harbin 150080, China.
Ji-Lin
JL
Li
Xiao-Ping
XP
Wang
Li
L
Zhong
Xiang-Ling
XL
Xu
D002871:Chromosome Banding; D023902:Chromosome Pairing; D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D017404:In Situ Hybridization, Fluorescence; D012434:Secale / Q000235:genetics*; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*