Tissue engineering of small intestinal tissue using collagen sponge scaffolds seeded with smooth muscle cells.
In a previously reported attempt to regenerate small intestine with autologous tissues, collagen scaffolds were used without cell seeding or with autologous mesenchymal stem cell seeding. However the regenerated intestine lacked a smooth muscle layer. To accomplish regeneration of a smooth muscle layer, this present study used collagen scaffolds seeded with the smooth muscle cells (SMC) in a canine model. Autologous SMC were isolated from stomach wall and cultured. Two types of scaffolds were fabricated: in SMC (+), cultured SMCs were mixed with collagen solution and poured into a collagen sponge; and in SMC (-), SMCs were omitted. Both scaffolds were implanted into defects of isolated ileum as a patch graft. Animals were euthanized at 4, 8, and 12 weeks; for the last time point, the ileal loop had been reanastomosed at 8 weeks. At 12 weeks, the SMC (-) group showed a luminal surface covered by a regenerated epithelial cell layer with very short villi; however only a thin smooth muscle layer was observed, representing the muscularis mucosae. In the SMC (+) group, the luminal surface was covered completely by a relatively well-developed epithelial layer with numerous villi. Implanted SMCs were seen in the lamina propria and formed a smooth muscle layer. Thus, we concluded that collagen sponge scaffolds seeded with autologous SMCs have a potential for small intestine regeneration.
Department of Surgery and Regenerative Medicine, Division of Surgery and Physiology of Digestive System, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan. yuen-n@koto.kpu-m.ac.jp
Yuen
Y
Nakase
Akeo
A
Hagiwara
Tatsuo
T
Nakamura
Syuichi
S
Kin
Susumu
S
Nakashima
Tetsuji
T
Yoshikawa
Ken-Ichirou
K
Fukuda
Yoshiaki
Y
Kuriu
Kouji
K
Miyagawa
Chohei
C
Sakakura
Eigo
E
Otsuji
Yasuhiko
Y
Shimizu
Yoshihito
Y
Ikada
Hisakazu
H
Yamagishi
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry*; D004285:Dogs; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D020007:Implants, Experimental; D007421:Intestine, Small / Q000166:cytology*; D045164:Mesenchymal Stem Cell Transplantation; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000166:cytology* / Q000648:ultrastructure; D013270:Stomach; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate