Simple extraction method and gas chromatography-mass spectrometry in the selective ion monitoring mode for the determination of phenols in wine.
The concentrations of 22 components of wine, including most of those that have been shown to possess significant biological properties, have been determined by a fast and simple analytical method based on gas chromatography with mass spectrometric detection in the selective ion monitoring mode (GC/MS-SIM). The procedure involves an easy liquid-liquid extraction and derivatization methods of flavanols, phenols and carboxylic acid, using a very small wine volume. The average recovery (Rcv) ranged from 73 to 107% and linear regression coefficients (r(2)) were in a range of 0.981<or=r(2)>or=0.999. The GC/MS-SIM technique gives good specificity and sensitivity, and can therefore be suitable for routine monitoring of the concentration of individual phenolic antioxidants during winemaking and the aging process.
Minuti
Lucio
L
Dipartimento di Chimica, Università degli Studi di Perugia, Via Elce di Sotto 8, 06123 Perugia, Italy. lucio@unipg.it
Pellegrino
Roberto Maria
RM
Tesei
Ilaria
I
D005591:Chemical Fractionation / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D012680:Sensitivity and Specificity; D014920:Wine / Q000032:analysis*
D010636:Phenols