Large-scale gene discovery in the pea aphid Acyrthosiphon pisum (Hemiptera).
Aphids are the leading pests in agricultural crops. A large-scale sequencing of 40,904 ESTs from the pea aphid Acyrthosiphon pisum was carried out to define a catalog of 12,082 unique transcripts. A strong AT bias was found, indicating a compositional shift between Drosophila melanogaster and A. pisum. An in silico profiling analysis characterized 135 transcripts specific to pea-aphid tissues (relating to bacteriocytes and parthenogenetic embryos). This project is the first to address the genetics of the Hemiptera and of a hemimetabolous insect.
INRA Rennes, UMR INRA-Agrocampus BiO3P, BP 35327, F-35653 Le Rheu Cedex, France.
Beatriz
B
Sabater-Muñoz
Fabrice
F
Legeai
Claude
C
Rispe
Joël
J
Bonhomme
Peter
P
Dearden
Carole
C
Dossat
Aymeric
A
Duclert
Jean-Pierre
JP
Gauthier
Danièle Giblot
DG
Ducray
Wayne
W
Hunter
Phat
P
Dang
Srini
S
Kambhampati
David
D
Martinez-Torres
Teresa
T
Cortes
Andrès
A
Moya
Atsushi
A
Nakabachi
Cathy
C
Philippe
Nathalie
N
Prunier-Leterme
Yvan
Y
Rahbé
Jean-Christophe
JC
Simon
David L
DL
Stern
Patrick
P
Wincker
Denis
D
Tagu
D000818:Animals; D001042:Aphids / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D018895:Microsatellite Repeats; D018532:Pisum sativum / Q000469:parasitology; D010802:Phylogeny; D010935:Plant Diseases / Q000469:parasitology; D011156:Population Density; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004247:DNA