Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium.
pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient. Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.
Itaya
Mitsuhiro
M
Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan. mita2001@sfc.keio.ac.jp
Sakaya
Nagayoshi
N
Matsunaga
Satoko
S
Fujita
Kyoko
K
Kaneko
Shinya
S
D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D003227:Conjugation, Genetic / Q000187:drug effects* / Q000502:physiology*; D003470:Culture Media / Q000494:pharmacology*; D007700:Kinetics; D010957:Plasmids / Q000187:drug effects / Q000235:genetics*
D003470:Culture Media