Characterization and cloning of T24, a Taenia solium antigen diagnostic for cysticercosis.
The third and final diagnostic antigen of the lentil lectin purified glycoproteins (LLGP) extracted from the larval stage of Taenia solium has been characterized, cloned, and expressed. T24 is an integral membrane protein that belongs to the tetraspanin superfamily. It migrates at a position corresponding to 24-kDa and as a homodimer at 42-kDa. Antibodies from cysticercosis patients recognize secondary structure epitopes that are dependent upon correctly formed disulfide bonds. A portion of T24, the large, extracellular loop domain, was expressed in an immunologically reactive form in insect cells. When tested in a Western blot assay with a large battery of serum samples, this protein, T24H, has a sensitivity of 94% (101/107), for detecting cases of cysticercosis with two or more viable cysts, and a specificity of 98% (284/290). The identification and expression of T24H sets the stage for the development of an ELISA suitable for testing single samples and for large-scale serosurveys that is not dependent upon the isolation and purification of antigens from parasite materials.
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. khancock@cdc.gov
Kathy
K
Hancock
Sowmya
S
Pattabhi
Fatima W
FW
Whitfield
Melinda L
ML
Yushak
William S
WS
Lane
Hector H
HH
Garcia
Armando E
AE
Gonzalez
Robert H
RH
Gilman
Victor C W
VC
Tsang
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D003001:Cloning, Molecular; D003551:Cysticercosis / Q000175:diagnosis* / Q000469:parasitology; D018509:DNA, Helminth / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D017422:Sequence Analysis, DNA; D041201:Taenia solium / Q000235:genetics / Q000276:immunology*
D000947:Antigens, Helminth; D018509:DNA, Helminth; D011994:Recombinant Proteins