Enabling glycosyltransferase evolution: a facile substrate-attachment strategy for phage-display enzyme evolution.
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
Kerry Routenberg
KR
Love
Jonathan G
JG
Swoboda
Christopher J
CJ
Noren
Suzanne
S
Walker
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D001435:Bacteriophages / Q000737:chemistry; D002236:Carbohydrate Conformation; D002384:Catalysis; D019020:Directed Molecular Evolution / Q000379:methods*; D004789:Enzyme Activation; D017351:N-Acetylglucosaminyltransferases / Q000737:chemistry*; D019151:Peptide Library; D017279:Selenocysteine / Q000737:chemistry*; D013997:Time Factors
D001425:Bacterial Outer Membrane Proteins; D019151:Peptide Library; D017279:Selenocysteine; D017351:N-Acetylglucosaminyltransferases; C064057:UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase