[Detection of genetically modified organisms in food and animal feed by polymerase chain reaction].
OBJECTIVE
To investigate the presence of genetically modified organisms (GMO) in the foods and animal feed samples in Guangzhou market.


METHODS
The presence of GMO were investigated by PCR detection of camv 35S promoter and nos terminator, and the presence of RoundUp Ready Soybean (RRS), Bt176 Maximaizer or Mon810 YieldGard in GMO-positive samples were further determined by PCR detecting their specific DNA fragments respectively.


RESULTS
One corn soup sample, two soybean samples, one potato fries sample as well as two animal feed samples were revealed to be GMO-positive in twenty-two food samples and three animal feed samples, and the presence of RRS in the GMO-positive soybean samples and the two positive animal feed samples were verified by PCR detection of a 129 bp RRS-specific DNA fragment, however, no Bt176 Maximaizer or Mon810 YieldGard specific PCR products were obtained with the GMO-positive corn soup and animal feed DNA samples used as PCR templates.


CONCLUSIONS
Genetically modified organism presented in foods and animal feeds even though they were not been labelled.
Zhou
Jian-chang
JC
Toxicological Laboratory, Guangdong Center for Disease Control and Prevention, Guangzhou 510300, China.
Yang
Ming-jie
MJ
Yang
Xing-fen
XF
Huang
Jun-ming
JM
D000821:Animal Feed / Q000032:analysis*; D018744:DNA, Plant / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D030841:Food, Genetically Modified; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013025:Glycine max / Q000235:genetics; D003313:Zea mays / Q000235:genetics
D018744:DNA, Plant