Applications of alginate in bioseparation of proteins.
Alginate is a polysaccharide that is a block polymer consisting of block units of guluronic acid and mannuronic acid. It shows inherent biological affinity for a variety of enzymes such as pectinase, lipase, phospholipase D, a and ss amylases and glucoamylase. Taking advantage of its precipitation with Ca2+ and the above-mentioned property, alginate has been used for purification of these enzymes by affinity precipitation, aqueous two phase separation, macroaffinity ligand facilitated three phase partitioning, immobilized metal affinity chromatography and expanded bed affinity chromatography. Thus, this versatile marine resource has tremendous potential in bioseparation of proteins.
Jain
Sulakshana
S
Chemistry Department, Indian Institute of Technology, New Delhi, India.
Mondal
Kalyani
K
Gupta
Munishwar N
MN
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002845:Chromatography; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008024:Ligands
D000464:Alginates; D004798:Enzymes; D006603:Hexuronic Acids; D008024:Ligands; D020723:Glucuronic Acid