Determination of the binding affinities of plasmid DNA using fluorescent intercalators possessing an acridine skeleton.
Novel acridine derivatives, wherein the steric factor has been varied systematically through substitution at the 9 position of the acridine ring, were evaluated as convenient fluorescent probes for nucleic acid detection. The binding affinities of N-(9-acridinylthiocarbamoyl)amino acids (ATA) with plasmid DNA (pUC 19) were investigated using UV-vis spectrophotometry, fluorometric titration and quantum chemical calculations (AM1). From spectrofluorometric analysis, the binding constants for the DNA-ATA complexes were determined. To elucidate its DNA intercalation, the most preferable tautomeric structure of ATA was established by means of AM1 calculations.
Sabolová
D
D
Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, P.J. Safárik University, 041 54 Kosice, Slovakia. sabdan@kosice.upjs.sk
Kozurková
M
M
Kristian
P
P
Danihel
I
I
Podhradský
D
D
Imrich
J
J
D000166:Acridines / Q000737:chemistry / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D010957:Plasmids; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000166:Acridines; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D007364:Intercalating Agents