[Protective effects of hirudin on acute experimental intracerebral hemorrhage].
OBJECTIVE
To explore the protective effects of hirudin on acute experimental intracerebral hemorrhage (ICH) by observing the changes of histologic pathology and brain water content as well as GFAP-positive cells in the perihematomal brain regions.


METHODS
The models of rat ICH were made with infusion of autologous blood into the right neucleus caudatus. The rats were divided randomly into control group, intracerebral hemorrhage group and treating group with hirudin. Brain water content was measured, and pathological and GFAP changes were observed.


RESULTS
The pathological impairation after ICH were gradually deteriorated and peaked at the third day. Brain water content after ICH was gradually increased and obviously after one day(P < 0.05) and peaked at the third day. GFAP-positive cells were gradually increased and peaked at the seventh day after ICH. In the treating groups, the pathological impairation and brain water content as well as the GFAP-positive cells were decreased as compared to those in the intracerebral hemorrhage group and the control group. And the positive correlation between GFAP-positive cell numbers and brain water content were shown by linear regression.


CONCLUSIONS
The local administration of hirudin, a special inhibitor of thrombin, has protective effects within the first week after ICH.
The First Affilated Hospital of Jilin University, Changchun, China.
Ying
Y
Zhang
Jia-chun
JC
Feng
Jiang
J
Wu
Ya-ping
YP
Ge
Wei-hua
WH
Zhang
Li-hua
LH
Hu
Wei
W
Han
D000208:Acute Disease; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D002543:Cerebral Hemorrhage / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006629:Hirudins / Q000494:pharmacology*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D005904:Glial Fibrillary Acidic Protein; D006629:Hirudins; D018696:Neuroprotective Agents