Seungma-galgeun-tang attenuates proinflammatory activities through the inhibition of NF-kappaB signal pathway in the BV-2 microglial cells.
Seungma-galgeun-tang (SGT) has been used for treatment of chronic diseases in the folk medicine recipe. Since nitric oxide (NO) is one of the major inflammatory parameters, we first studied the effects of SGT on NO production in lipopolysaccharide (LPS)-stimulated BV-2 microglia. SGT inhibited the secretion of NO in BV-2 microglia, without affecting cell viability. The protein level of inducible nitric oxide synthase (iNOS) was decreased by SGT and SGT also inhibited production of PGE(2) and expression of Cox-2. Proinflammatory cytokines, such as TNF-alpha, IL-1beta and IL-12, were inhibited by SGT in a dose-dependent manner and SGT blocked the activation of NF-kappaB, which was considered to be a potential transcription factor for the expression of iNOS, COX-2 and proinflammatory cytokines. SGT also blocked the degradation of IkappaB and activation (decrease of cytosolic p65) of NF-kappaB, p65. These results suggest that SGT could exert its anti-inflammatory actions by suppressing the synthesis of NO through inhibition of NF-B activity.
Lyu
Sun Ae
SA
Department of Oriental Medicine, College of Oriental Medicine, Dongeui University, Busan, Republic of Korea.
Lee
Seung Yeon
SY
Lee
Song Jin
SJ
Son
Sung Woo
SW
Kim
Mun Ok
MO
Kim
Gi-Young
GY
Kim
Young Hee
YH
Yoon
Hwa Jung
HJ
Kim
Hoon
H
Park
Dong Il
DI
Ko
Woo Shin
WS
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000187:drug effects; D017931:DNA Primers; D015232:Dinoprostone / Q000037:antagonists & inhibitors; D015536:Down-Regulation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006868:Hydrolysis; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors; D051379:Mice; D017628:Microglia / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics
D017931:DNA Primers; D004365:Drugs, Chinese Herbal; D018836:Inflammation Mediators; D016328:NF-kappa B; D012333:RNA, Messenger; C000611873:seungma-galgeun-tang; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D015232:Dinoprostone