[A simplified method of determination of cAMP in plants using modified enzyme immunoassay].
A modification of enzyme immunoassay for quantification of cAMP and its derivatives in plants is suggested, which is based on the use of cAMP-specific primary rabbit antibodies and secondary goat antibodies conjugated with peroxidase. The lower threshold of this method is 10 pM. This technique is highly specific, simple, and inexpensive.
L A
LA
Lomovatskaia
A S
AS
Romanenko
N V
NV
Krivolapova
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000242:Cyclic AMP / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques / Q000379:methods*; D010944:Plants / Q000737:chemistry*; D011817:Rabbits
D000906:Antibodies; D000242:Cyclic AMP