Effects of amino acid on morphological development and nucleus formation of arachidonic acid-producing filamentous micro-organism, Mortierella alpina.
OBJECTIVE
Effects of amino acid on morphological development and nucleus formation of arachidonic acid-producing filamentous micro-organism, Mortierella alpina were investigated using flow-through chamber.


RESULTS
Mortierella alpina CBS 754.68 was cultivated in flow through chamber using nutrient-rich, minimal and specific amino acid-containing minimal media. To investigate the effect of amino acid on morphological parameters either 0.28 g l(-1) alanine, 0.53 g l(-1) sodium glutamate one hydrate or 0.42 g l(-1) valine was added to the minimal medium. In a flow-through chamber, the growth of hyphal elements and nucleus formation of arachidonic acid-producing fungus M. alpina were studied on-line, using image analysis techniques. When the Ala- and Val-containing media were used, the hyphal growth units (HGUs) were 90.2 and 86.7 microm per tip, respectively, which were 2.4-fold higher than that in the nutrient-rich medium, indicating that Ala and Val stimulate the elongation of hyphae. The specific nucleus formation rates were Glu->Val-containing media>minimal and nutrient-rich media>Ala-containing medium. The nucleus doubling times in Glu- and Val-containing media were 1.9 and 2 h, respectively, which were not significant different.


CONCLUSIONS
Ala and Val stimulate the elongation of M. alpina hyphae, and nucleus formation rates were Glu->Val->Ala-containing media.


CONCLUSIONS
Formation of fungal morphology and nucleus were shown using the flow-through chamber coupled with image analysis, which making possible to discuss the relationship between mycelial morphology and nucleus formation of M. alpina.
Koizumi
K
K
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan.
Higashiyama
K
K
Park
E Y
EY
D000409:Alanine / Q000378:metabolism / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000096:biosynthesis*; D002467:Cell Nucleus / Q000187:drug effects*; D003470:Culture Media; D018698:Glutamic Acid / Q000378:metabolism; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D020094:Mortierella / Q000187:drug effects / Q000254:growth & development*; D012970:Sodium Glutamate / Q000378:metabolism / Q000494:pharmacology; D014633:Valine / Q000378:metabolism / Q000494:pharmacology
D000596:Amino Acids; D003470:Culture Media; D016718:Arachidonic Acid; D018698:Glutamic Acid; D014633:Valine; D000409:Alanine; D012970:Sodium Glutamate