Comparison of estimates for volumes of brain ablations derived from structural MRI and classical histology.
Estimates of auditory cortex ablation sizes in a rodent model as derived from classical histology (volume reconstructions from Nissl-stained brain sections) and structural magnetic resonance imaging (MRI) (T1-weighted whole-brain scans from a 4.7 T animal scanner) were compared in the same specimens (Mongolian gerbils). Estimates of lesion volumes obtained with the two methods were very similar, robust, highly correlated and not significantly different from each other. Hence, the general usefulness of structural MRI for the determination of brain lesion size in small animal models is demonstrated. MRI therefore seems to be well suited to determine proper size and location of an experimental brain ablation prior to (potentially extensive) behavioral testing.
Deutscher
Anke
A
Leibniz Institute for Neurobiology, Department Auditory Learning & Speech, 39118 Magdeburg, Germany.
Niessen
Heiko G
HG
Angenstein
Frank
F
Goldschmidt
Jürgen
J
Scheich
Henning
H
Schulze
Holger
H
D000818:Animals; D001303:Auditory Cortex / Q000293:injuries / Q000473:pathology; D001930:Brain Injuries / Q000473:pathology*; D004396:Coloring Agents; D003627:Data Interpretation, Statistical; D005849:Gerbillinae; D006653:Histology; D008279:Magnetic Resonance Imaging
D004396:Coloring Agents