Changes induced in colonocytes by extensive intestinal resection in rats.
After massive intestinal resection, physiological compensatory events occur in the remnant small bowel and in the colon. The aim of our work was to study the propensity of the colon to evolve after a massive small bowel resection in rats. The resected group, where 80% of the small bowel length was removed, was compared with sham-operated rats (transected). During the 7 postoperative days, rats were fed orally or they received an elemental nutrition through a gastric catheter. PepT1 and NHE3 mRNAs encoding apical membrane transporters were not modified in the present experiment. However, two unexpected genes (I-FABP and UroR) were up-regulated in the colon following intestinal resection. These modifications occurred without an imbalance of cell cycle protein content and in a context of low short-chain fatty acid production.
Laboratoire de Nutrition et de sécurité alimentaire, INRA, Doaine de Vilvert, 78350, Jouy en Josas, France.
Hubert
H
Lardy
Muriel
M
Thomas
Marie-Louise
ML
Noordine
Aurélia
A
Bruneau
Claire
C
Cherbuy
Pierre
P
Vaugelade
Catherine
C
Philippe
Virginie
V
Colomb
Pierre-Henri
PH
Duee
D000818:Animals; D002432:Cecum / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D003106:Colon / Q000378:metabolism* / Q000473:pathology; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000378:metabolism; D007421:Intestine, Small / Q000601:surgery*; D008297:Male; D000074011:Peptide Transporter 1; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D055293:Receptors, Urokinase Plasminogen Activator; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*; D027981:Symporters / Q000235:genetics / Q000378:metabolism*
D018797:Cell Cycle Proteins; D050556:Fatty Acid-Binding Proteins; D005232:Fatty Acids, Volatile; D000074011:Peptide Transporter 1; C524900:Plaur protein, rat; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; C489940:Slc15a1 protein, rat; C000613387:Slc9a3 protein, rat; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers; D027981:Symporters