Primary organ function of warm ischaemically damaged porcine kidneys after retrograde oxygen persufflation.
BACKGROUND
As warm ischaemic damage is a major reason for the loss of donor organs, an experimental study was performed in order to evaluate retrograde oxygen persufflation (ROP) as a method to extend the warm ischaemic tolerance of kidneys.


METHODS
Kidneys of 32 pigs were exposed to warm ischaemia for 60, 90 or 120 min. Then, 16 kidneys were subjected to ROP for 4 h at 4 degrees C and 16 controls were stored in cold UW-solution, followed by autotransplantation.


RESULTS
Only in the group with 60 min warm ischaemic time and ROP did all animals survive the observation period of 7 days. In all other groups some animals died due to anuria. Short-term survivors in these groups had significantly higher creatinine levels.


CONCLUSIONS
In this setting, ROP was superior to cold storage when applied after 60 min of warm ischaemia. Clinical evaluation of ROP in the setting of marginal donors and non-heartbeating donation is recommended.
Treckmann
Jürgen W
JW
Department of General, Visceral and Transplantation Surgery, University Hospital of Essen, Hufelandstr.55,45122 Essen, Germany. j.treckmann@gmx.net
Paul
Andreas
A
Saad
Stefano
S
Hoffmann
Julia
J
Waldmann
Karl-Heinz
KH
Broelsch
Christoph E
CE
Nagelschmidt
Manfred
M
D000818:Animals; D003404:Creatinine / Q000378:metabolism; D005260:Female; D007511:Ischemia; D007668:Kidney / Q000378:metabolism*; D016030:Kidney Transplantation; D009926:Organ Preservation / Q000379:methods*; D010100:Oxygen / Q000378:metabolism*; D013552:Swine; D013696:Temperature; D013997:Time Factors; D020858:Tissue and Organ Harvesting / Q000379:methods*
D003404:Creatinine; D010100:Oxygen