A general approach for antibody purification utilizing [Protein A-catechol:Fe3+] macro-complexes.
A general platform for antibody purification utilizing free nonimmobilized Protein A modified with the strong metal chelator catechol (ProA-CAT) and Fe3+ ions is presented. The mechanism of purification requires formation and precipitation of macro-complexes composed of [ProA-CAT:IgG:Fe3+]. Target IgGs are eluted directly from the precipitates (i.e. pellets) at pH 3 in high yields (71-80%) and high purity (>95%), without dissociating the [ProA-CAT:Fe3+] insoluble macro-complex.
Affisink Biotechnology Ltd, 11 Hamaccabee St. Kiryat-Ono 55572, Israel. guy@affisink.com
Guy
G
Patchornik
Amnon
A
Albeck
D000818:Animals; D002396:Catechols / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007501:Iron / Q000737:chemistry*; D015394:Molecular Structure; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000737:chemistry*
D002396:Catechols; D002614:Chelating Agents; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D007501:Iron; C034221:catechol