Differentially displayed expressed sequence tags in Melipona scutellaris (Hymenoptera, Apidae, Meliponini) development.
We have compared gene expression, using the Differential Display Reverse Transcriptase-Polymerase Chain Reaction (DDRT-PCR) technique, by means of mRNA profile in Melipona scutellaris during ontogenetic postembryonic development, in adult worker, and in both Natural and Juvenile Hormone III-induced adult queen. Six, out of the nine ESTs described here, presented differentially expressed in the phases L1 or L2, or even in both of them, suggesting that key mechanisms to the development of Melipona scutellaris are regulated in these stages. The combination HT11G-AP05 revealed in L1 and L2 a product which matches to thioredoxin reductase protein domain in the Clostridium sporogenes, an important protein during cellular oxidoreduction processes. This study represents the first molecular evidence of differential gene expression profiles toward a description of the genetic developmental traits in the genus Melipona.
Santana
Flávia A
FA
Laboratório de Genética, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, 38400-902 Uberlândia, MG, Brazil.
Nunes
Francis M F
FM
Vieira
Carlos U
CU
Machado
Maria Alice M S
MA
Kerr
Warwick E
WE
Silva
Wilson A
WA
Bonetti
Ana Maria
AM
D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000235:genetics* / Q000254:growth & development; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D007605:Juvenile Hormones / Q000235:genetics*; D007814:Larva / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D007605:Juvenile Hormones; D012333:RNA, Messenger