The antiepileptic drug carbamazepine affects sodium transport in toad epithelium.
The present work investigates the effects of the antiepileptic drug carbamazepine (CBZ) on sodium transport in the isolated skin of the toad Pleurodema thaul. A submaximal concentration of the drug (0.2 mM) applied to the outer surface of the epithelium increased the electrical parameters short-circuit current (Isc) and potential difference (PD) by over 28%, whereas only a higher concentration (1 mM) induced over a 45% decrease in these parameters when applied to the inner surface. The amiloride test showed that the outer surface stimulatory effect was accompanied by an increase and the inner surface inhibitory effect by a decrease in the sodium electromotive force (ENa). Exploration of these effects of CBZ on the outer surface showed that 0.2 mM increased net Na+ (22Na) influx by 20% and 0.6 mM CBZ decreased Na+ mucosa-serosa flux by 19%, a result in agreement with the finding that higher concentrations of CBZ applied to the inner surface not only decreased ENa but also sodium conductance (GNa).
Suwalsky
Mario
M
Department of Polymers, Faculty of Chemical Sciences, University of Concepción, E. Larenas 129, Casilla 160-C, Concepción, Chile. msuwalsk@udec.cl
Mennickent
Sigrid
S
Norris
Beryl
B
Cardenas
Hernán
H
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D000927:Anticonvulsants / Q000633:toxicity*; D002025:Bufonidae; D002220:Carbamazepine / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D017136:Ion Transport / Q000187:drug effects; D008297:Male; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*
D000927:Anticonvulsants; D002220:Carbamazepine; D000584:Amiloride; D012964:Sodium