Gene expressions of canine angiopoietin-1 and -2 in normal tissues and spontaneous tumours.
The angiopoietin (Ang) family of proteins are central to the regulation of angiogenesis. The purposes of this study were to determine cDNA sequences of canine Ang-1 and Ang-2 and investigate their expressions in normal tissues and spontaneous tumours. The cDNA sequences of canine Ang-1 and Ang-2 were 1,494 and 1,488 bp, and the deduced amino acid sequences were 497 and 495 residues, respectively. The cDNA sequences of canine Ang-1 and Ang-2 showed high homology with those of the other mammalian species. Canine Ang-1 and Ang-2 mRNA were detectable in all 22 normal tissues and spontaneous tumours. Higher mRNA expression level of canine Ang-2 was demonstrated in mammary simple carcinomas, haemangiosarcoma and hepatocellular carcinoma in comparison with normal tissues.
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252-8510, Japan.
Y
Y
Kato
K
K
Asano
I
I
Mizutani
T
T
Konno
Y
Y
Sasaki
K
K
Kutara
K
K
Teshima
K
K
Edamura
R
R
Kano
K
K
Suzuki
H
H
Shibuya
T
T
Sato
A
A
Hasegawa
S
S
Tanaka
D000595:Amino Acid Sequence; D042683:Angiopoietin-1 / Q000096:biosynthesis* / Q000235:genetics; D042702:Angiopoietin-2 / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D004283:Dog Diseases / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D015870:Gene Expression; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism / Q000662:veterinary; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics
D042683:Angiopoietin-1; D042702:Angiopoietin-2; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A