Human, insect and nematode caspases kill Saccharomyces cerevisiae independently of YCA1 and Aif1p.
This study characterised the impact of active metazoan apoptotic proteases (caspases) on Saccharomyces cerevisiae viability. Expression of active caspase-3 or caspase-8 in yeast ruptured plasma and nuclear membranes and dramatically impaired clonogenic survival, but did not damage DNA. Deletion of the proposed yeast apoptosis regulators YCA1 or Aif1p did not affect the ability of human, insect or nematode caspases to kill yeast. These data indicate that expression of active metazoan caspases causes irreversible damage to yeast membranes and organelles, in a manner independent of YCA1 and Aif1p.
Children's Cancer Centre, Royal Children's Hospital, Parkville, 3052, Australia.
M A
MA
Puryer
C J
CJ
Hawkins
D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D017173:Caenorhabditis elegans / Q000201:enzymology; D053148:Caspase 3; D053181:Caspase 8; D020169:Caspases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002470:Cell Survival; D004330:Drosophila / Q000201:enzymology; D017353:Gene Deletion; D006801:Humans; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D019076:Transgenes
D000199:Actins; D029701:Saccharomyces cerevisiae Proteins; C443623:AIF1 protein, S cerevisiae; D009247:NADH, NADPH Oxidoreductases; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8; D020169:Caspases; C456272:MCA1 protein, S cerevisiae