Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4+ and CD8+ T cells to nasal killed-bacillus calmette-guerin in mice.
On single nasal immunization of mice with killed-bacillus calmette-guerin (BCG) plus a mutant Escherichia coli enterotoxin, delayed-type hypersensitivity was induced and BCG-infection decreased. Spleen cells, particularly CD4+ T cells among them produced IL-2, IFNgamma and TNFalpha in response to the killed-BCG or purified protein derivatives. CD8+ T cells including cytotoxic T lymphocytes produced IFNgamma and TNFalpha. However, both types of T cells reacted a little to Ag85B. The mutant induces cellular immunity to nasal killed-BCG vaccine and decreases BCG-infection. CD4+ and CD8+ T cells produce cytokines effective for tuberculosis. Although killed-BCG loses some antigens like Ag85B, nasal killed-BCG plus the mutant is useful for tuberculosis.
Department of Microbiology, Fujita Health University, School of Medicine, Toyoake, Aichi 470-1192, Japan.
Hiroki
H
Takahashi
Keiko
K
Sasaki
Miyuki
M
Takahashi
Naoko
N
Shigemori
Shyunichi
S
Honda
Hideyuki
H
Arimitsu
Sadayuki
S
Ochi
Naoya
N
Ohara
Takao
T
Tsuji
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000281:Administration, Intranasal; D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D001427:Bacterial Toxins / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D004768:Enterotoxins / Q000494:pharmacology*; D029968:Escherichia coli Proteins / Q000494:pharmacology*; D053222:Fas Ligand Protein; D005260:Female; D006968:Hypersensitivity, Delayed / Q000209:etiology; D018928:Immunity, Mucosal; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008809:Mice, Inbred C3H; D008813:Mice, Inbred ICR; D009154:Mutation; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D018417:Th1 Cells / Q000276:immunology*; D048069:Tumor Necrosis Factors / Q000096:biosynthesis; D015164:Vaccines, Inactivated / Q000276:immunology
D000276:Adjuvants, Immunologic; D001500:BCG Vaccine; D001427:Bacterial Toxins; D016207:Cytokines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D048069:Tumor Necrosis Factors; D015164:Vaccines, Inactivated; D054353:Perforin; C027538:heat-labile enterotoxin, E coli