Influence of global fluorination on chloramphenicol acetyltransferase activity and stability.
Varied levels of fluorinated amino acid have been introduced biosynthetically to test the functional limits of global substitution on enzymatic activity and stability. Replacement of all the leucine (LEU) residues in the enzyme chloramphenicol acetyltransferase (CAT) with the analog, 5',5',5'-trifluoroleucine (TFL), results in the maintenance of enzymatic activity under ambient temperatures as well as an enhancement in secondary structure but loss in stability against heat and denaturants or organic co-solvents. Although catalytic activity of the fully substituted CAT is preserved under standard reaction conditions compared to the wild-type enzyme both in vitro and in vivo, as the incorporation levels increase, a concomitant reduction in thermostability and chemostability is observed. Circular dichroism (CD) studies reveal that although fluorination greatly improves the secondary structure of CAT, a large structural destabilization upon increased levels of TFL incorporation occurs at elevated temperatures. These data suggest that enhanced secondary structure afforded by TFL incorporation does not necessarily lead to an improvement in stability.
Department of Chemical and Biological Sciences, Polytechnic University, 6 Metrotech Center, Brooklyn, NY 11201, USA.
Tatyana
T
Panchenko
Wan Wen
WW
Zhu
Jin Kim
JK
Montclare
D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis* / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology*; D005461:Fluorine / Q000737:chemistry*; D007930:Leucine / Q000031:analogs & derivatives* / Q000737:chemistry; D013696:Temperature
D005461:Fluorine; C035177:5',5',5'-trifluoroleucine; D015500:Chloramphenicol O-Acetyltransferase; D007930:Leucine