Suppression of T lymphocyte activity by lupeol isolated from Crataeva religiosa.
Lupeol has been shown to possess antiarthritic activity through possible suppression of the immune system. As seen in the following studies, it was found to suppress various immune factors such as the phagocytic (cell-killing) activity of macrophages, T-lymphocyte activity that included CD4+T cell mediated cytokine generation. Assessment of T cells and their intracellular content of cytokines was carried out by flow cytometric analysis in Balb/c mice. Oral administration of lupeol at doses of 12.5-200 mg/kg p.o. inhibited CD4+ T and CD8+ T cell counts and cytokines IL-2, IFN-gamma (Th1) and IL-4 (Th2). Cytometric bead array (CBA) technology was applied to carry out simultaneous measurement of multiple serum cytokines. The oral LD(0) in mice was more than 2 g/kg body weight.
Department of Pharmacology, Division of Pharmacology, Regional Research Laboratory, Jammu Tawi, 180001, India. sarangbhani@rediffmail.com
Sarang
S
Bani
Anpurna
A
Kaul
Beenish
B
Khan
Sheikh Fayaz
SF
Ahmad
K A
KA
Suri
B D
BD
Gupta
N K
NK
Satti
G N
GN
Qazi
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects*; D031483:Capparaceae / Q000737:chemistry; D016207:Cytokines / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D006968:Hypersensitivity, Delayed; D007118:Immunoassay; D007371:Interferon-gamma / Q000037:antagonists & inhibitors; D007376:Interleukin-2 / Q000037:antagonists & inhibitors; D015847:Interleukin-4 / Q000037:antagonists & inhibitors; D051379:Mice; D008807:Mice, Inbred BALB C; D053978:Pentacyclic Triterpenes; D010587:Phagocytosis / Q000187:drug effects; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000094042:Lupanes
D016207:Cytokines; D007371:Interferon-gamma; D007376:Interleukin-2; D015847:Interleukin-4; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; C010480:lupeol; D000094042:Lupanes