Xenopus Xotx2 and Drosophila otd share similar activities in anterior patterning of the frog embryo.
Despite the obvious anatomical differences between the fly and the vertebrate body plans, several genes involved in their development are largely conserved. In this work we provide evidence that overexpression of the Drosophila orthodenticle (otd) gene in Xenopus laevis has a similar effect to that of its homolog Xotx2. Injections of otd mRNA in whole embryos lead to posterior truncations and to induction of ectopic cement glands, similar to Xotx2 injections. In animal cap assays, otd, like Xotx2, is able to activate the cement gland marker XAG and to suppress the expression of the epidermal marker XK81. Finally, as assayed by Einsteck transplantation assays, otd, like Xotx2, is able to respecify a tail/trunk organizer to a head organizer. In this work we also show that Xotx2 and otd share molecular functions that regulate early regional specification of the Xenopus anterior neural plate. Gain-of-function experiment targeting low doses of either otd or Xotx2 mRNAs in the neural plate promote reduction of Xrx1 and Xbf1 expression domain; no changes are observed for the anterior mesodermal marker Xgsc, the dorsal diencephalic marker Xbh1, and the midbrain/hindbrain marker Xen2. otd/Xotx2 inhibition activity of Xrx1 and Xbf1 expression is consistent with the strong inhibition of Xfgf8 expression in the anterior neural ridge observed upon otd/Xotx2 mRNA injection.
Dipartimento di Biologia, Laboratorio di Biologia Cellulare e dello Sviluppo, Università di Pisa, via G Carducci 13, 56100, Pisa, Italy.
Andrea
A
Lunardi
Robert
R
Vignali
D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005123:Eye / Q000196:embryology; D005136:Eye Proteins; D051524:Fibroblast Growth Factor 8 / Q000378:metabolism; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D009420:Nervous System / Q000196:embryology; D051857:Otx Transcription Factors / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics; D029867:Xenopus Proteins / Q000235:genetics / Q000502:physiology*; D014982:Xenopus laevis / Q000033:anatomy & histology / Q000196:embryology* / Q000235:genetics
D029721:Drosophila Proteins; D005136:Eye Proteins; C117087:FOXG1 protein, Xenopus; D018398:Homeodomain Proteins; D051857:Otx Transcription Factors; C497661:Otx2 protein, Xenopus; D012333:RNA, Messenger; C524416:Rax protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins; C093053:oc protein, Drosophila; D051524:Fibroblast Growth Factor 8