[Expression of ETV5 and MMP-7 in early stage cervical squamous cell carcinoma and its role in lymphatic metastasis].
OBJECTIVE
Transcription factor Ets variant gene 5 (ETV5) participates in tumor metastasis, and matrix metalloproteinase-7 (MMP-7) also plays an important role in tumor invasion and metastasis. This study was to investigate the expression of ETV5 and MMP-7 in cervical squamous cell carcinoma, and explore the roles of ETV5 and MMP-7 in invasion and metastasis of early stage cervical squamous cell carcinoma.


METHODS
Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of ETV5 and MMP-7 mRNA in 86 samples of cervical squamous cell carcinoma and 21 samples of normal cervix, and immunohistochemistry was used to detect the expression of MMP-7 protein in these samples.


RESULTS
The mRNA levels of ETV5 and MMP-7 were significantly higher in cervical cancer tissues than in normal cervix tissues (0.507+/-0.073 vs. 0.201+/-0.057, P<0.01; 0.488+/-0.078 vs. 0.305+/-0.064, P<0.01), and were significantly higher in nodal metastasis group than in no nodal metastasis group (0.538+/-0.063 vs. 0.496+/-0.073, P<0.05; 0.542+/-0.066 vs. 0.468+/-0.072, P<0.01). The protein level of MMP-7 was significantly higher in cervical cancer tissues than in normal cervix tissues (3.826+/-1.427 vs. 1.714+/-0.383, P<0.01), and was significantly higher in nodal metastasis group than in no nodal metastasis group (4.935+/-1.614 vs. 2.623+/-1.086, P<0.01). The positive rate of MMP-7 protein was significantly higher in lymph node metastasis group than in no lymph node metastasis group (65.21% vs. 36.51%, P<0.01). ETV5 expression was positively correlated to MMP-7 expression (r=0.221, P<0.05).


CONCLUSIONS
ETV5 and MMP-7 are highly expressed in metastatic cervical squamous cell carcinoma, and may be served as markers in estimating the invasive and metastatic potential of cervical squamous cell carcinoma.
Department of Gynecology, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, P. R. China. shwu1215@tom.com
Su-Hui
SH
Wu
Jing
J
Zhang
Ying
Y
Li
Jian-Min
JM
Li
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D020783:Matrix Metalloproteinase 7 / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology
D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C086680:ETV5 protein, human; D012333:RNA, Messenger; D014157:Transcription Factors; D020783:Matrix Metalloproteinase 7