ShyA, a membrane protein for proper septation of hyphae in Streptomyces.
The life cycle of Streptomyces involves the formation of filamentous substrate and aerial hyphae. Following cessation of growth of an aerial hypha, multiple septation occurs at the tip to produce a chain of unigenomic spores. A gene, shyA, which influences several aspects of this growth, was isolated and partially characterized in Streptomyces coelicolor. The gene product is a representative of a well-conserved family of small actinomycete proteins. The shyA mutant sporulates normally but displays hyper septum formation and altered spore-chain morphology. Biochemical separation experiments and immunofluorescence staining demonstrated that the shyA gene product locates at cell membranes. Moreover, yeast two-hybrid screen and GST-pull-down assay showed that ShyA can interact with itself. Altogether, ShyA belongs to a new family of membrane-associated proteins which plays a role in morphological differentiation in actinomycetes.
Tseng
Shun-Fu
SF
Department of Microbiology, College of Medicine, National Taiwan University, Taipei 10063, Taiwan.
Huang
Tzu-Wen
TW
Chen
Carton W
CW
Chern
Ming-Kai
MK
Tam
Ming F
MF
Teng
Shu-Chun
SC
D000595:Amino Acid Sequence; D049109:Cell Proliferation; D048429:Cell Size; D025301:Hyphae / Q000378:metabolism* / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000378:metabolism* / Q000648:ultrastructure
D008565:Membrane Proteins