Inactivation of vibrio parahaemolyticus and vibrio vulnificus in phosphate-buffered saline and in inoculated whole oysters by high-pressure processing.
Inactivation studies for Vibrio parahaemolyticus TX-2103 (serotype O3:K6) and Vibrio vulnificus MO-624 (clinical isolate) were conducted in phosphate-buffered saline (PBS) and in inoculated oysters under high-pressure processing conditions. V. parahaemolyticus was more resistant than V. vulnificus in PBS at all pressures and times. A 6-log reduction of V. parahaemolyticus and V. vulnificus in PBS at 241 MPa required 11 and 5 min, respectively, which included a 3-min pressure come-up time. A 4.5-log reduction of V. parahaemolyticus in oysters at 345 MPa required 7.7 min, which included a 6.7-min pressure come-up time. More than a 5.4-log reduction of V. vulnificus in oysters at 345 MPa occurred during the 6-min pressure come-up time. Both V. parahaemolyticus and V. vulnificus in PBS and in oysters were reduced to nondetectable numbers at 586 MPa during the 8- and 7-min pressure come-up times, respectively.
Virginia Seafood Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University, Virginia Sea Grant Program, 102 South King Street, Hampton, Virginia 23693, USA.
Jaheon
J
Koo
Michael L
ML
Jahncke
Paul W
PW
Reno
Xiaopei
X
Hu
Parameswarakumar
P
Mallikarjunan
D000818:Animals; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D006801:Humans; D010125:Ostreidae / Q000382:microbiology*; D011312:Pressure; D012758:Shellfish / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000187:drug effects / Q000254:growth & development*; D041261:Vibrio vulnificus / Q000187:drug effects / Q000254:growth & development*