[Effects of ultrasound induction on genomic DNA of housefly larvae].
The genomic DNA of housefly larvae was extracted after ultrasound induction, and the structure was analyzed by UV, fluorescence, IR and 1H NMR. The 3'-end of attacin gene was sequenced and compared by means of PCR. All the results indicated that ultrasound induction can destroy the second structure and the base stacking of genomic DNA of housefly larvae, which will result in mismatch repair during DNA duplication and finally change the sequence of DNA, but it has no significant effect on chemical groups and chemical band of genomic DNA.
College of Life Science, Nankai University, Tianjin 300071, China.
Chun-Ju
CJ
An
Feng
F
Li
Li-Jin
LJ
Zhang
De-Sen
DS
Li
Rong-Qian
RQ
Du
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D049750:Genome, Insect; D006793:Houseflies / Q000737:chemistry* / Q000235:genetics; D007814:Larva / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D013057:Spectrum Analysis; D014465:Ultrasonics
D004247:DNA