[Changes in P-selectin expression after brain injury in rats].
OBJECTIVE
To explore the relationship between expression thange of P-selectin after brain injury and secondary brain damage.


METHODS
Sixty SD rats were randomized into 3 equal groups, namely the control group, mild injury group and severe injury group and animal models of brain injury were established in SD rats according to the method of Feeney. P-selectin expression in the brain tissues were determined at 6 h and l, 3, and 7 days following brain injury (n=5 for each time point). Imaging analysis was performed using computerized imaging technique.


RESULTS
P-selectin expression and neutrophil infiltration in the brain tissues increased significantly 6 h after brain injury (P<0.05), reaching the peak level at postoperative 24 h and then gradually decreased.


CONCLUSIONS
P-selectin expression and neutrophil infiltration increase significantly following brain injury, and the time course and distribution of P-selectin expression are consistent with the secondary damage of the brain, strongly suggesting the involvement of P-selectin upregulation in the secondary insult after brain injury.
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China. zhangrj1972@sina.com
Rong-jun
RJ
Zhang
Chao
C
You
Bo-wen
BW
Cai
Min
M
He
Yong-bo
YB
Yang
Zhan
Z
Liu
Qing-fang
QF
Jiao
D000818:Animals; D001923:Brain Chemistry; D001930:Brain Injuries / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D019007:P-Selectin / Q000096:biosynthesis*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D019007:P-Selectin