Amino Acid Sequence Around the Catalytic Site in Glyceraldehyde-3-Phosphate Dehydrogenase from Bacillus stearothermophilus.
The tryptic peptide containing the active-site cysteine in glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus 1503 was isolated after inhibition of the enzyme with (14)C-iodoacetate. The amino acid sequence of the 20-residue peptide was determined by 19 successive cycles of dansyl-Edman degradation. The sequence shows considerable homology with its counterparts from mesophilic sources but differs by the addition of Ala-His-His at the N-terminus and by the substitution of phenylalanine for leucine in the prototype sequence.
Bridgen
J
J
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, England, Departments of Microbiology and Biochemistry, and the McIlvain Laboratories, University of Kansas Medical Center, Kansas City, Kansas 66103.
Harris
J I
JI
McDonald
P W
PW
Amelunxen
R E
RE
Kimmel
J R
JR