[Mechanisms of contractile reaction changes and efficiency of oxygen utilization by portal vein smooth muscles in experimental diabetes mellitus].
On the rat portal vein isolated strips the essential reduction of contractive response, increase of stiffness of vascular smooth muscles at their stretching and increase of oxygen value of smooth muscles' work are revealed in conditions of experimental diabetes mellitus. The possible mechanism of such infringements is chronic hyperglycemia, which results in development of oxidative stress and reduction of NO synthesis by constitutive NOS. Indeed, the significant decrease of activity of constitutive NO-synthase and increase of the oxidative stress markers contents are shown at experimental diabetes mellitus. The similar infringements are marked at in vitro model of hyperglycemia, blockade of NO-synthase and in conditions of oxidative stress. Additional confirmation of a role of NO synthesis blockade and oxidative stress in development of such infringements at diabetes is fact that application of antioxidant melatonin and L-arginine, a precursor of NO to diabetic animals results in partial restoration of contractive reactions, reduction of stiffness and oxygen value of smooth muscles' work.
Prysiazhna
O D
OD
Kotsiuruba
A V
AV
Tkachenko
M M
MM
Sahach
V F
VF
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000503:physiopathology*; D005466:Fluorocarbons / Q000378:metabolism*; D006842:Hydrocarbons, Brominated; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D011169:Portal Vein / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D011921:Rats, Inbred WKY
D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D007527:Isoenzymes; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III; C003072:perflubron