Molecular cloning and characterization of a novel MAP kinase gene in Chorispora bungeana.
Chorispora bungeana Fisch. and C.A. Mey (Chorispora bungeana) is a rare alpine subnival plant species that is highly capable of resisting freezing environment. Since it is a stress-tolerant plant, we investigated the participation of mitogen-activated protein kinases (MAPKs) as possible mediators of abiotic stresses. We have isolated from Chorispora bungeana a new MAPK cDNA CbMAPK3 which encodes a 369 amino-acid protein with moderate to high nucleotide sequence similarity to previously reported plant MAPK genes. CbMAPK3 contains all 11 of the MAPK conserved subdomains and the phosphorylation motif TEY. The transcripts of CbMAPK3 were detected and no tissue-specific expression were observed in both roots and leaves, The transcripts of CbMAPK3 accumulated highly and rapidly when Chorispora bungeana treated with cold (4 and -4 degrees C), ABA and salinity stress. These results indicate that the CbMAPK3 may play an important role in response to environmental stresses.
Zhang
Tengguo
T
Key Laboratory of Arid and Grassland Agroecology, Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Liu
Yubing
Y
Xue
Lingui
L
Xu
Shijian
S
Chen
Tuo
T
Yang
Tongwen
T
Zhang
Lijing
L
An
Lizhe
L
D000040:Abscisic Acid / Q000737:chemistry; D000595:Amino Acid Sequence; D001483:Base Sequence; D019607:Brassicaceae / Q000201:enzymology*; D003001:Cloning, Molecular; D003080:Cold Temperature; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010802:Phylogeny; D018515:Plant Leaves / Q000201:enzymology; D018517:Plant Roots / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism; D012492:Salts / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid
D012333:RNA, Messenger; D012492:Salts; D000040:Abscisic Acid; D020928:Mitogen-Activated Protein Kinases