Enantiocomplementary reduction of 3-phenylthiopropan-2-one by Bacillus sp.: effect of medium components.
The enantioselectivity of the enzymes responsible for reduction of prochiral compound 3-phenylthiopropan-2-one was dependent on the concentration of yeast extract and glucose in the growth medium. Low concentrations of yeast extract (0.1-0.9% w/v) favored the formation of S-enantiomer (62% ee at 0.1% w/v yeast extract) of 3-phenylthiopropan-2-ol. However, R-enantiomer of the reduced product was formed when MSM was supplemented with yeast extract at a concentration of 1% (w/v) or more with a maximum ee of 85% at 2.0% (w/v) yeast extract supplement in the growth medium.
Rampal
Poonam
P
Department of Microbiology, Guru Nanak Dev University, Amritsar 143 005, India.
Saini
Harvinder Singh
HS
Raj
Mayil
M
Chimni
Swapandeep Singh
SS
Chadha
Bhupinder Singh
BS
D001407:Bacillus / Q000378:metabolism*; D001711:Biotransformation; D007659:Ketones / Q000737:chemistry / Q000378:metabolism*; D008968:Molecular Conformation; D015394:Molecular Structure; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism*
C517697:3-phenylthiopropan-2-one; D007659:Ketones; D013438:Sulfhydryl Compounds