Construction of a multivalent vaccine strain of Shigella flexneri and evaluation of serotype-specific immunity.
Shigella flexneri causes more fatalities by shigellosis than any other Shigella species. There are 13 different serotypes of S. flexneri and their distribution varies between endemic geographical regions. The immune response against S. flexneri is serotype-specific, so current immunization strategies have required the administration of multiple vaccine strains to provide protection against multiple serotypes. In this study, we report the construction of a multivalent S. flexneri vaccine strain, SFL1425, expressing the O-antigen structure specific for serotypes 2a and 5a. This combination of type antigens has not previously been reported for S. flexneri. The multivalent vaccine strain, SFL1425 was able to induce a specific immune response against both serotypes 2a and 5a in a mouse pulmonary model.
School of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra, Australia.
Amy V
AV
Jennison
Fleur
F
Roberts
Naresh K
NK
Verma
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004405:Dysentery, Bacillary / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D019081:O Antigens / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction; D022281:Shigella Vaccines / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D012763:Shigella flexneri / Q000235:genetics / Q000276:immunology*; D013045:Species Specificity; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D019081:O Antigens; D022281:Shigella Vaccines; D014613:Vaccines, Attenuated