Severe acute respiratory syndrome diagnostics using a coronavirus protein microarray.
To monitor severe acute respiratory syndrome (SARS) infection, a coronavirus protein microarray that harbors proteins from SARS coronavirus (SARS-CoV) and five additional coronaviruses was constructed. These microarrays were used to screen approximately 400 Canadian sera from the SARS outbreak, including samples from confirmed SARS-CoV cases, respiratory illness patients, and healthcare professionals. A computer algorithm that uses multiple classifiers to predict samples from SARS patients was developed and used to predict 206 sera from Chinese fever patients. The test assigned patients into two distinct groups: those with antibodies to SARS-CoV and those without. The microarray also identified patients with sera reactive against other coronavirus proteins. Our results correlated well with an indirect immunofluorescence test and demonstrated that viral infection can be monitored for many months after infection. We show that protein microarrays can serve as a rapid, sensitive, and simple tool for large-scale identification of viral-specific antibodies in sera.
Molecular, Cellular, and Developmental Biology and Comparative Medicine, and Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT 06520, USA.
Heng
H
Zhu
Shaohui
S
Hu
Ghil
G
Jona
Xiaowei
X
Zhu
Nate
N
Kreiswirth
Barbara M
BM
Willey
Tony
T
Mazzulli
Guozhen
G
Liu
Qifeng
Q
Song
Peng
P
Chen
Mark
M
Cameron
Andrea
A
Tyler
Jian
J
Wang
Jie
J
Wen
Weijun
W
Chen
Susan
S
Compton
Michael
M
Snyder
D000465:Algorithms; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood; D002170:Canada / Q000453:epidemiology; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008969:Molecular Sequence Data; D040081:Protein Array Analysis / Q000379:methods* / Q000706:statistics & numerical data; D020543:Proteome; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification
D000914:Antibodies, Viral; D020543:Proteome; D014764:Viral Proteins