Psc-AFP, an antifungal protein with trypsin inhibitor activity from Psoralea corylifolia seeds.
An antifungal protein designated as Psc-AFP, with an apparent molecular mass of 18kDa, was isolated from a traditional Chinese herb, malaytea scurfpea (Psoralea corylifolia L.). The isolation procedure entailed extraction, cation exchange chromatography on CM FF, gel filtration chromatography on Superdex 75 and reversed-phase high performance liquid chromatography on SOURCE 5RPC column. Automated Edman degradation determined the partial N-terminal sequence of Psc-AFP to be NH2-EWEPVQNGGSSYYMVPRIWA, which displayed homology with plant trypsin inhibitors. The protease inhibitor activity of Psc-AFP was then confirmed by the inhibition on trypsin. Psc-AFP at 10 microM inhibited the mycelial growth of Alternari brassicae, Aspergillus niger, Fusarium oxysporum and Rhizoctonia cerealis, suggesting that Psc-AFP has a role in the defense against pathogens.
Yang
Xingyong
X
Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture, Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, PR China. yangxy@swau.edu.cn
Li
Jun
J
Wang
Xiaowen
X
Fang
Weiguo
W
Bidochka
Michael J
MJ
She
Rong
R
Xiao
Yuehua
Y
Pei
Yan
Y
D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology*; D002412:Cations; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000494:pharmacology; D029907:Psoralea / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D014357:Trypsin / Q000737:chemistry; D014361:Trypsin Inhibitors / Q000494:pharmacology*
D000935:Antifungal Agents; D002412:Cations; D010940:Plant Proteins; D014361:Trypsin Inhibitors; D014357:Trypsin