Gadolinium-loaded polychelating polymer-containing cancer cell-specific immunoliposomes.
Liposome loading with Gd via the membrane-incorporated polychelating amphiphilic polymers (PAPs) significantly increases the Gd content and relaxivity (T1 parameter) of PEGylated liposomes, which can be used as contrast agents for magnetic resonance imaging (MRI). Here, we demonstrate that such Gd-containing liposomes can be additionally modified with the monoclonal anticancer antibody 2C5 (mAb 2C5) possessing the nucleosome(NS)-restricted specificity via the PEG spacer. Liposome-bound antibody preserves its specific activity (ELISA) and such Gd-loaded PEGylated 2C5-immunoliposomes specifically recognize various cancer cells in vitro and target an increased amount of Gd to their surface compared to antibody-free Gd-liposomes or Gd-liposomes modified with tumor nonspecific antibody. Gd-loaded cancer cell-targeted immunoliposomes may represent promising agents for enhanced tumor MRI.
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Suna
S
Erdogan
Aruna
A
Roby
Rishikesh
R
Sawant
Jim
J
Hurley
Vladimir P
VP
Torchilin
D000911:Antibodies, Monoclonal / Q000276:immunology; D002614:Chelating Agents / Q000737:chemistry*; D003287:Contrast Media; D004797:Enzyme-Linked Immunosorbent Assay; D005682:Gadolinium / Q000737:chemistry*; D008081:Liposomes; D008279:Magnetic Resonance Imaging; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D011108:Polymers / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D003287:Contrast Media; D008081:Liposomes; D011108:Polymers; D005682:Gadolinium