[RAPD analysis for genetic diversity of Chrysanthemum morifolium].
OBJECTIVE
To investigate the genetic diversity of Chrysanthemum morifolium at the level of molecular biology.


METHODS
The total genomic DNA was extracted from medicinal chrysanthemums by 2% CTAB method. And the genetic diversity of 22 C. morifolium accessions was tested by RAPD marks. The NTSYS software was used to analyze the marks.


RESULTS
26 10-mer arbitrary primers were found to acquire polymophic results. A total of 233 bands were amplified, of which 89.7% bands were found to be polymophic. 8.04 polymophic bands were amplified by each primer on the average. The results of cluster analysis by using UPGMA method showed that all the tested accessions could be differentiated by RAPD marks.


CONCLUSIONS
There actually existed much genetic diversity at the molecular level among the germplasm resources of C. morifolium. RAPD marks could be effective tools to construct DNA fingerprintings of C. morifolium. The differences between the tested chrysanthemums are related to the environments. However, it was affected by genetic facters more significantly.
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, China.
Wen-bin
WB
Xu
Qiao-sheng
QS
Guo
Chang-lin
CL
Wang
D002681:China; D031202:Chrysanthemum / Q000145:classification / Q000235:genetics*; D016000:Cluster Analysis; D018744:DNA, Plant / Q000235:genetics*; D017753:Ecosystem; D010802:Phylogeny; D010946:Plants, Medicinal / Q000235:genetics*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D018744:DNA, Plant