Kinetic changes of oxidative stress and selenium status in plasma and tissues following burn injury in selenium-deficient and selenium-supplemented rats.
BACKGROUND
This study investigates the relationship between the burn-induced oxidative stress and the selenium status.


METHODS
The rats were fed with a selenium-adequate diet or a selenium-depleted diet for 5 weeks, before a third-degree thermal injury was applied to the animals. One group of selenium-depleted animals received injections of sodium selenite after the injury. The selenium status and the oxidative stress parameters were measured for 5 days.


RESULTS
The selenium-deficient diet leads to oxidative stress with a high stimulation of the superoxide dismutase activity. After the burn injury, the oxidative stress appears important because the initial selenium status is already impaired and, in all animals, the selenium levels and the antioxidant seleno-dependent glutathione peroxidase (GPx) activity decrease in the plasma and the tissues. A treatment with daily selenium injections is efficient in normalizing selenium levels and restores the GPx activity, but fails to counteract the initial oxidative damages induced by the selenium-deficient diet.


CONCLUSIONS
The selenium status before the burn injury is a modulating factor of the burn-induced oxidative stress. A single selenium supplement is not sufficient to counteract these oxidative damages and henceforth combined antioxidant supplementations should be investigated to improve the early treatment of the burn patients.
Sandre
Caroline
C
Laboratoire de Nutrition, Vieillissement et Maladies Cardiovasculaires, Faculté de Pharmacie, La Tronche, France.
Agay
Diane
D
Ducros
Véronique
V
Faure
Henri
H
Cruz
Catherine
C
Alonso
Antonia
A
Chancerelle
Yves
Y
Roussel
Anne-Marie
AM
D000818:Animals; D002056:Burns / Q000503:physiopathology*; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008297:Male; D009751:Nutritional Requirements; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000172:deficiency*; D018038:Sodium Selenite / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D012643:Selenium; D018038:Sodium Selenite