Phylogenetic analysis of envelope glycoprotein (E1) gene of rubella viruses prevalent in Japan in 2004.
We performed a molecular epidemiological study on the envelope glycoprotein gene (E1 gene) obtained by PCR amplification from specimens of 17 rubella patients in certain areas (Gunma, Saitama, and Kagoshima prefectures, and Tokyo metropolitan area) in Japan in 2004. In these sequences of partially amplified DNAs (283 bases) within the E1 gene, no nucleotide substitution was observed. They were classified into genotype 1D of clade 1 in the constructed phylogenetic tree. One amino acid substitution was found between the amino acid sequence predicted from these DNAs and those of Japanese strains [To-336 vaccine strain (To-336 vac) and its wild progenitor (To-336 wt)]. The results suggest that the rubella viruses (RV) prevalent in certain areas of Japan in 2004 were highly homologous and were closely related with Japanese vaccine strain.
Gunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Gunma 371-0052, Japan.
Mika
M
Saitoh
Naomi
N
Shinkawa
Shinichi
S
Shimada
Yukari
Y
Segawa
Kenji
K
Sadamasu
Michiya
M
Hasegawa
Masahiko
M
Kato
Kunihisa
K
Kozawa
Tsuyoshi
T
Kuramoto
Osamu
O
Nishio
Hirokazu
H
Kimura
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012409:Rubella / Q000453:epidemiology* / Q000821:virology; D012412:Rubella virus / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins; C061826:E1 envelope protein, Rubella virus