The relative merits of the tetO2 and tetO7 promoter systems for the functional analysis of heterologous genes in yeast and a compilation of essential yeast genes with tetO2 promoter substitutions.
We have generated a collection of yeast strains, each of which has an essential yeast gene under the control of the tetracycline-responsive, tetO, promoter. Screens using first-generation promoter-swap strains uncovered the non-specific responsiveness of the tetO7 promoter to a known human transcription factor (hIRF-1). Non-specific regulation was not observed with the tetO2 promoter. Reporter assays have been used to demonstrate this phenomenon. Subsequent efforts to generate a collection of tetracycline-regulatable strains have focused on the tetO2 promoter. These strains are available to the yeast community and can be used for functional genomics studies.
Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK.
Jill A
JA
Wishart
Michael
M
Osborn
Manda E
ME
Gent
Kuangyu
K
Yen
Zorana
Z
Vujovic
Paul
P
Gitsham
Nianshu
N
Zhang
J
J
Ross Miller
Stephen G
SG
Oliver
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics* / Q000502:physiology; D006801:Humans; D050856:Interferon Regulatory Factor-1 / Q000235:genetics; D007763:Lac Operon; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004271:DNA, Fungal; D050856:Interferon Regulatory Factor-1; C083139:Tet O resistance protein, Bacteria