[Experimental study on cryopreservation of seeding cells of tissue engineered tendons].
This study sought to find out a good way for the cryopreservation of tendon seeding cells so as to facilitate the preparation of tissue engineering tendons as products. The related questions are how different factors affect cell survival rate at the procedure of preservation and whether cryopreservation affects seeding cells' biological characters as well as collagen secretive function. The results of experiment indicate that DMSO is a more effective cryoprotectant in cryopreservation of tissue engineered tendon seeding cells. Blood serum nourishment is very important in cell culture, preservation and treatment. The same sustenance after cryopreservation increases cell survival rate. In the process of cryopreservation, the concentration of cells is important to cell survival rate; cell survival rate will decrease when it is less than 1.0 x 10(6)/ml. In the process of cryopreservation, the cooling speed is also important to cell survival rate, slow cooling method achieves higher cell survival rate than does the rapid cooling method. Cryopreservation by use of 10%DMSO+15%FCS+75%DMEM does not affect seeding cells' collagen secretive function greatly and does not affect seeding cells' growth curve, cell cycle and chromosome mode obviously. The prescription of 10%DMSO +15%FCS+75%DMEM is suited for the cryopreservation of tendon seeding cells.
Division of Stem Cell and Tissue Engineering, Key Laboratory of Biotherapy of Human Diseases, Educational Ministry P.R. China.
Xiaoqi
X
Zhu
Zhiming
Z
Yang
Huiqi
H
Xie
Tingwu
T
Qin
D002452:Cell Count; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D004121:Dimethyl Sulfoxide; D006801:Humans; D018482:Muscle, Skeletal / Q000166:cytology; D013710:Tendons / Q000166:cytology*; D023822:Tissue Engineering; D014021:Tissue Preservation / Q000379:methods
D004121:Dimethyl Sulfoxide