AROS-29 is involved in adaptive response to oxidative stress.
Transient adaptation to mild oxidative stress was induced in human osteosarcoma cells chronically grown in sub-toxic concentrations of diethylmaleate (DEM), a glutathione (GSH) depleting agent. The adapted cells, compared to untreated cells, contain increased concentrations of GSH (4-6 fold) which, upon DEM withdrawal from the culture medium, return to normal values and are more resistant to subsequent oxidizing stress induced either by toxic concentrations of the same agent or by (H(2)O(2)) treatment. To investigate the molecular mechanisms involved in the adaptive response to oxidative stress, we analyzed the gene expression profiles of DEM-adapted cells by differential display. The expression of adaptive response to oxidative stress (AROS)-29 gene, coding for a transmembrane protein of unknown function, as well as of some known genes involved in energy metabolism, protein folding and membrane traffic is up-regulated in adapted cells. The increased resistance to both DNA damage and apoptosis, in cells stably overexpressing AROS-29, demonstrated its functional role in the protection against oxidative stress.
Università di Napoli Federico II, Dipartimento di Biochimica e Biotecnologie Mediche, Naples, Italy.
Nicola
N
Montesano Gesualdi
Giuseppa
G
Chirico
Maria Teresa
MT
Catanese
Giuseppe
G
Pirozzi
Franca
F
Esposito
D000222:Adaptation, Physiological / Q000187:drug effects / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D020552:Comet Assay; D005434:Flow Cytometry; D015870:Gene Expression; D020869:Gene Expression Profiling; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008298:Maleates / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D008298:Maleates; D008565:Membrane Proteins; D012333:RNA, Messenger; C014476:diethyl maleate; D005978:Glutathione