Effects of swimming training and free mobilization on bone mineral densities of rats with the immobilization-induced osteopenia.
OBJECTIVE
To investigate the possible effects of regular swimming exercise on bone mineral density (BMD) compared with free activity after cast immobilization of rats.


METHODS
We carried out the study from April 2005 to June 2005 at the Department of Sports Medicine, Medical School of Suleyman Demirel University, Isparta, Turkey. The study included a total of 24 female Wistar rats. The rats were randomized to control (n = 6), swimming training (ST) n = 9, and free mobilization (FM) n = 9 groups. We measured Bone mineral densities of femur and vertebra of all rats with a total body scanner using software specifically designed for small animals, before study started and at weeks 3 and 7. Timepoints corresponded to basal, after cast removal (ACIM), and after 3 weeks of free mobilization (AFM) or swimming training (AST). We immobilized the right hindlimb of each ST and FM animal with a cast while the left hindlimbs were kept free. After 3 weeks, the casts were removed. Then we allowed the rats to move freely in their cage for one week, after which the animals in ST group started to swim for 5 days a week for 3 weeks for 30 minutes per day. The group FM rats moved freely in the cage.


RESULTS
Bone mineral density of the femur and vertebra after cast immobilization was significantly decreased compared with both their basal and age-matched control group. After mobilization, significant increases occurred in both groups according to ACIM. Similar but milder changes were observed in free limbs femur BMD of rats. Interestingly, vertebra BMD of swimming group was also higher than its age-matched control group (p<0.05).


CONCLUSIONS
Our study showed that swimming exercise had a significant rehabilitative effect on BMD loss associated with immobilization. Further studies are needed to investigate the effects of swimming on other bone properties.
Department of Sports Medicine, Suleyman Demirel University Faculty of Medicine, Isparta, Turkey. hilmi@med.sdu.edu.tr
Hilmi
H
Karatosun
Ali
A
Erdogan
Mustafa
M
Yildiz
Celal
C
Akgun
Cem
C
Cetin
D000818:Animals; D015519:Bone Density; D001851:Bone Diseases, Metabolic / Q000209:etiology / Q000517:prevention & control*; D005260:Female; D007103:Immobilization / Q000009:adverse effects; D010805:Physical Conditioning, Animal; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013550:Swimming