A bovine glucuronidase for assembly of beta-D-glucuronyl-(1-3)-6-O-sulfo-beta-D-gluco- and galacto-pyranosyl linkages.
Glucuronidase-catalyzed transglycosylation was examined by using 4-nitrophenyl beta-D-glucuronide (D-GlcA-O-pNP) as the glycosyl donor; when pNP 6-O-sulfo-beta-D-gluco- and D-galacto-pyranosides were used as the acceptors, a bovine enzyme was found to construct beta-D-GlcA-(1-3)-linkages with the 6-O-sulfo-sugars in both a site- and beta-selective way.
Uzawa
Hirotaka
H
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba 305-8565, Japan. h.uzawa@aist.go.jp(HU)
Nagatsuka
Takehiro
T
Hiramatsu
Hideo
H
Nishida
Yoshihiro
Y
D000818:Animals; D002384:Catalysis; D002417:Cattle; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D005966:Glucuronidase / Q000378:metabolism*; D006031:Glycosylation; D015394:Molecular Structure
D004187:Disaccharides; C518141:beta-D-glucuronyl-(1-3)-6-O-sulfo-beta-D-galactopyranosyl; C518140:beta-D-glucuronyl-(1-3)-6-O-sulfo-beta-D-glucopyranosyl; D005966:Glucuronidase