[Multiple isoforms of cyclophilin A associated with human immunodeficiency virus type 1].
It is well-known that a peptidyl-prolyl cis-trans isomerase cyclophilin A (CyPA) is incorporated into Human immunodeficiency virus type 1 (HIV-1) particle. The proteome analysis of the purified HIV-1 strain LAV-1 (HIV-1(LAV-1)) reveals that three isoforms of CyPA with an isoelectric point (pI) of 6.00, 6.40, and 6.53 are inside the viral membrane and another isoform with a pI of 6.88 is outside the viral membrane; and that the CyPA isoform with a pI of 6.53 is N-acetylated. The mechanisms that permit the redistribution of CyPA with a pI of 6.88 on the viral surface have not yet been clarified, but it penetrates the viral membrane after budding.
Misumi
Shogo
S
Department of Pharmaceutical Biochemistry, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Japan. misumi@gpo.kumamoto-u.ac.jp
D000818:Animals; D021984:Cyclophilin A / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007525:Isoelectric Focusing; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D020543:Proteome; D014763:Viral Matrix Proteins; D019065:Virus Assembly / Q000235:genetics
D020033:Protein Isoforms; D020543:Proteome; D014763:Viral Matrix Proteins; D021984:Cyclophilin A