Taenia asiatica and Taenia saginata: genetic divergence estimated from their mitochondrial genomes.
We conducted a differential identification of Taenia asiatica and Taenia saginata, through the mapping of mitochondrial genomes and the sequencing of the cox1 and cob genes. The entire mitochondrial genomes of T. asiatica and T. saginata were amplified by long-extension PCR and cloned; each was approximately 14 kb in size. Restriction maps of T. asiatica and T. saginata mitochondrial genomes were then constructed using 13 restriction enzymes. The resulting restriction patterns enable us to estimate their genetic divergence at 4.8%. The actual sequence divergence was computed 4.5% from the cox1 gene, and 4.1% from the cob gene. These results support the designation of T. asiatica as a separate species from T. saginata.
Department of Parasitology and Medical Research Institute, Chungbuk National University College of Medicine, Chongju, Chungbuk 361-763, Republic of Korea.
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Jeon
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D000818:Animals; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D016678:Genome; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013621:Taenia / Q000235:genetics*; D041202:Taenia saginata / Q000235:genetics*
D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D045303:Cytochromes b; D003576:Electron Transport Complex IV