Solid-phase extraction of 4(5)-methylimidazole (4MeI) and 2-acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)-imidazole (THI) from foods and beverages with subsequent liquid chromatographic-electrospray mass spectrometric quantification.
A method for the simultaneous determination of 4(5)-methylimidazole (4MeI) and 2-acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)-imidazole (THI) was developed using SPE and HPLC/MS. Solid-phase extraction using SCX Disc cartridges was used for isolation of the analytes from liquid samples. The lower LOQwas 0.1 ng/mL for 4MeI and 0.2 ng/ mL for THI. The linearity of the calibration curves was satisfactory as indicated by correlation coefficients >0.999. The CV for the intra- and inter-day precision was <5% (n = 6); the accuracy was in the range 98-103%. The recovery was > or = 97 and > or = 98% for THI and 4MeI, respectively. The method was used to determine THI and 4MeI in beverages, coffee, caramel colours and other samples.
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry Brno, Czech Republic.
Borivoj
B
Klejdus
Jitka
J
Moravcová
Lea
L
Lojková
Jan
J
Vacek
Vlastimil
V
Kubán
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis; D007093:Imidazoles / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization
D007093:Imidazoles; C049929:2-acetyl-4(5)-tetrahydroxybutylimidazole; C023439:4-methylimidazole