Detection of 100% of mutations in 124 individuals using a standard UV/Vis microplate reader: a novel concept for mutation scanning.
We report the development of a simple and inexpensive assay for the detection of DNA polymorphisms and mutations that is based on the modification of mismatched bases by potassium permanganate. Unlike the chemical cleavage of mismatch assay, which also exploits the reactivity of potassium permanganate to detect genomic variants, the assay we describe here does not require a cleavage manipulation and therefore does not require expensive or toxic chemicals or a separation step, as mismatches are detected using direct optical methods in a microplate format. Studies with individual deoxynucleotides demonstrated that the reactivity with potassium permanganate resulted in a specific colour change. Furthermore, studies with synthetic oligonucleotide heteroduplexes demonstrated that this colour change phenomenon could be applied to detect mismatched bases spectrophotometrically. A collection of plasmids carrying single point mutations in the mouse beta-globin promoter region was used as a model system to develop a functional mutation detection assay. Finally, the assay was validated as 100% effective in detecting mismatches in a blinded manner using DNA from patients previously screened for mutations using established techniques, such as sequencing, SSCP and denaturing high-performance liquid chromatography (DHPLC) analysis in DNA fragments up to 300 bp in length.
Genomic Disorders Research Centre Fitzroy, Victoria 3065, Australia. tania.tabone@adelaide.edu.au
Tania
T
Tabone
Georgina
G
Sallmann
Elizabeth
E
Webb
Richard G H
RG
Cotton
D000818:Animals; D020137:Base Pair Mismatch; D001483:Base Sequence; D001609:Beta-Globulins / Q000235:genetics; D004252:DNA Mutational Analysis / Q000295:instrumentation / Q000379:methods*; D003854:Deoxyribonucleotides / Q000737:chemistry; D020180:Heteroduplex Analysis / Q000379:methods; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D011196:Potassium Permanganate / Q000737:chemistry; D013053:Spectrophotometry / Q000295:instrumentation; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation
D001609:Beta-Globulins; D003854:Deoxyribonucleotides; D009841:Oligonucleotides; D011196:Potassium Permanganate