The hydrolipidic ratio in age-related maturation of adipose tissues.
The hydrolipidic ratio (HLR) expresses the amount of water and fat in a tissue. HLR can be studied non-invasively in the living organism and can be mapped in different areas of the body with high spatial and temporal resolution. In the present work we have evaluated the HLR in different adipose tissue depots in young or adult rats using tissue arrays of fat fragments by 1H-spectroscopy. In young animals, the highest percentage of water (33%) was found in the interscapular brown adipose tissue (iBAT). Mesenteric fat (mWAT) also appeared highly hydrated (24%). The deposits composed of epididymal, retroperitoneal and pelvic white adipose tissue (eWAT, rWAT and pWAT, respectively) contained an amount of water ranging from 14% to 17%. In adult animals, a reduction of the water content was found in all the depots. In e/r/pWAT, the age-related maturation was characterized by large changes in adipocyte diameter accompanied by a small change in HLR. In the iBAT, the maturation was accompanied by small change in adipocyte diameter and a greater diminution of HLR. mWAT showed an intermediate pattern between e/r/pWAT and iBAT. In all the studied depots, an age-related increase in leptin expression was found. This increase was relatively low in iBAT (40%) and high in the e/r/pWAT (204-273%). The work expand the knowledge about the physiological significance of the HLR by 1H-spectroscopy.
Section of Anatomy and Histology, Department of Morphological and Biomedical Sciences, Medical Faculty, University of Verona, Strada Le Grazie 8, 37134 Verona, Italy.
Isabella
I
Cavallini
Maria A
MA
Marino
Cristina
C
Tonello
Pasquina
P
Marzola
Elena
E
Nicolato
Paolo Francesco
PF
Fabene
Laura
L
Calderan
Paolo
P
Bernardi
Roberto M
RM
Asperio
Enzo
E
Nisoli
Andrea
A
Sbarbati
D017667:Adipocytes / Q000737:chemistry / Q000166:cytology; D000273:Adipose Tissue / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D001823:Body Composition; D001834:Body Water; D048429:Cell Size; D020738:Leptin / Q000378:metabolism; D008055:Lipids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D011522:Protons; D051381:Rats; D017208:Rats, Wistar; D046888:Tissue Array Analysis
D020738:Leptin; D008055:Lipids; D011522:Protons