Polysaccharide microarrays for polysaccharide-platelet-derived-growth-factor interaction studies.
Polysaccharide microarrays have great potential for the high-throughput analysis of polysaccharide-protein interactions. Here we demonstrate that a polysaccharide microarray prepared by printing a library of dextran polymers derivatized by methylcarboxylate, benzylamide, and sulfate groups (DMCBSu compounds) on to glass slides permitted the rapid identification of a set of compounds able to interact with the platelet-derived growth factor BB (PDGF-BB) isoform, a growth factor involved in wound healing. Microarray interaction results were compared to the capacity of DMCBSu compounds to potentiate the in vitro PDGF-BB-induced proliferation of human dermal fibroblasts.
Biological Institute of Lille, UMR 8161 CNRS, Université de Lille 1 et 2/Institut Pasteur de Lille, 1 rue du Pr Calmette 59021 Lille Cedex, France.
Olivier
O
Carion
Julien
J
Lefebvre
Guy
G
Dubreucq
Latifa
L
Dahri-Correia
José
J
Correia
Oleg
O
Melnyk
D000077214:Becaplermin; D049109:Cell Proliferation / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D008970:Molecular Weight; D019151:Peptide Library; D010982:Platelet-Derived Growth Factor / Q000737:chemistry* / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry*; D020574:Proto-Oncogene Proteins c-sis; D013499:Surface Properties
D003911:Dextrans; D019151:Peptide Library; D010982:Platelet-Derived Growth Factor; D011134:Polysaccharides; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin