Trace analysis of antibacterial tylosin A, B, C and D in honey by liquid chromatography-electrospray ionization-mass spectrometry.
A new LC-ESI-MS method was developed for the determination of residues of the antibacterial tylosins A, B, C and D in honey. The procedure employed an SPE on polymeric cartridges for the isolation of tylosins from diluted honey. Chromatographic separation of the tylosins was performed on a C18 column (150 x 4.60 mm2 ID, 5 microm) using a ternary gradient made of formic acid 1% in water (solvent A), methanol (solvent B) and ACN (solvent C) as mobile phase, at 30 degrees C and at a flow rate of 0.8 mL/min. Average analyte recoveries for the studied compounds ranged from 89 to 106% in replica sets of fortified honey samples. The detection limits for the four drugs studied were between 2 and 3 microg/kg. The developed method has been applied to the analysis of tylosin residues in honey from veterinarian treated beehives fed with the technical product, which contains the four compounds and is a new candidate antibiotic to treat American foulbrood disease of honey bee colonies.
Nozal Nalda
María Jesús
MJ
Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain. mjdnozal@qa.uva.es
Bernal Yagüe
José Luis
JL
Gómez
María Teresa Martín
MT
Jiménez Sevilla
Juan José
JJ
Bernal del Nozal
José
J
Higes Pascual
Mariano
M
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid; D006722:Honey / Q000032:analysis*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature; D015645:Tylosin / Q000032:analysis* / Q000737:chemistry
D000900:Anti-Bacterial Agents; D015645:Tylosin