Protection against reactive oxygen species injuries in rat isolated perfused hearts: effect of LPBNAH, a new amphiphilic spin-trap derived from PBN.
Numerous strategies have been designed to protect the myocardium against reactive oxygen species (ROS). The present study was designed to test wether LPBNAH, a new amphiphilic spin-trap derived from PBN is able to protect isolated perfused rat hearts against ROS injuries. Following total glola ischemia (30 min), hearts were reperfused in the presence or not of LPBNAH (10 micromol/l), and left ventricular function was continuously monitored. The addition of LPBNAH led to a significant recovery in left ventricular developped pressure (LVDevP, control: 16.5+/- 7.5, p < 0.05). To conclude, the present results strongly suggest that the modification of previous wellknown molecules in order to facilitate their access to intracellular site of ROS production might be of interest to limit oxidative stresses.
Tanguy
S
S
Laboratoire de Physiologie des Adaptations Cardiovasculaires à l'Exercice, JE 2426, Faculté des Sciences, Université d'Avignon, France. stephane.tanguy@univ-avignon.fr
Durand
G
G
Reboul
C
C
Polidori
A
A
Pucci
B
B
Dauzat
M
M
Obert
P
P
D000818:Animals; D003497:Cyclic N-Oxides; D004187:Disaccharides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D006339:Heart Rate / Q000187:drug effects; D007097:Imines / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D009589:Nitrogen Oxides / Q000737:chemistry / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D010477:Perfusion / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013113:Spin Labels; D019097:Spin Trapping; D013997:Time Factors; D016277:Ventricular Function, Left / Q000187:drug effects / Q000502:physiology; D017725:Ventricular Pressure / Q000187:drug effects
D003497:Cyclic N-Oxides; D004187:Disaccharides; D007097:Imines; C514717:LPBNAH compound; D018696:Neuroprotective Agents; D009589:Nitrogen Oxides; D017382:Reactive Oxygen Species; D013113:Spin Labels; C029217:phenyl-N-tert-butylnitrone