Two-dimensional electrophoresis protein profile of the phytopathogenic fungus Botrytis cinerea.
Botrytis cinerea is a phytopathogenic fungi causing disease in a number of important crops. It is considered a very complex species in which different populations seem to be adapted to different hosts. In order to characterize fungal virulence factors, a proteomic research was started. A protocol for protein extraction from mycelium tissue, with protein separation by 2-DE and MS analysis, was optimised as a first approach to defining the B. cinerea proteome. Around 400 spots were detected in 2-DE CBB-stained gels, covering the 5.4-7.7 pH and 14-85 kDa ranges. The averages of analytical and biological coefficients of variance for 64 independent spots were 16.1% and 37.5%, respectively. Twenty-two protein spots were identified by MALDI-TOF or ESI IT MS/MS, with some of them corresponding to forms of malate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase. Two more spots matched a cyclophilin and a protein with an unknown function.
Laboratory of Microbiology, Marine and Environmental Sciences Faculty, University of Cádiz, Cádiz, Spain.
Francisco Javier
FJ
Fernández-Acero
Inmaculada
I
Jorge
Enrique
E
Calvo
Inmaculada
I
Vallejo
María
M
Carbú
Emilio
E
Camafeita
Juan Antonio
JA
López
Jesús Manuel
JM
Cantoral
Jesús
J
Jorrín
D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000378:metabolism*; D013058:Mass Spectrometry; D010944:Plants / Q000382:microbiology*; D020543:Proteome / Q000378:metabolism*
D005656:Fungal Proteins; D020543:Proteome