Adiponectin protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity.
1-Methyl-4-phenylpyridinium ion (MPP+), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin because it elicits a severe Parkinson's disease-like syndrome characterized by elevation of intracellular reactive oxygen species level and apoptotic death. Adiponectin, secreted from adipose tissue, mediates systemic insulin sensitivity with liver and muscle as target organs. Adiponectin can also suppress superoxide generation in endothelial cells. In the present study, we investigated the protective effects of adiponectin on MPP+-induced cytotoxicity in human neuroblastoma SH-SY5Y cells, as well as the underlying mechanism. Our results suggest that the protective effects of adiponectin on MPP+-induced apoptosis may be ascribed to its anti-oxidative properties, anti-apoptotic activity via inducing expression of SOD and catalase, and regulation of Bcl-2 and Bax expression. These data indicated that adiponectin might provide a useful therapeutic strategy for the treatment of progressive neurodegenerative diseases such as Parkinson's disease.
The Brain Korea 21 Project for Medical Science, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea.
Tae Woo
TW
Jung
Ji Young
JY
Lee
Wan Sub
WS
Shim
Eun Seok
ES
Kang
Jong Sun
JS
Kim
Chul Woo
CW
Ahn
Hyun Chul
HC
Lee
Bong Soo
BS
Cha
D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology*; D052242:Adiponectin / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004338:Drug Combinations; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology*; D018696:Neuroprotective Agents / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*
D052242:Adiponectin; D004338:Drug Combinations; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D015655:1-Methyl-4-phenylpyridinium