No evidence of population structure across three isolated subpopulations of Russula brevipes in an oak/pine woodland.
Russula brevipes is common ectomycorrhizal (EM) fungus that is associated with several hosts across temperate forest ecosystems. A previous study has demonstrated that substructuring across large geographic distances (1500 km) occurs in the western USA. To examine genetic structure over a more localized scale, basidiocarps of Russula brevipes from three subpopulations, separated by distances of 230-1090 m, were collected over two consecutive years in an oak/pine woodland. Microsatellite loci were used to test for population differentiation both among subpopulations and by year. No significant population differentiation was detected between subpopulations (theta(ST) = 0.01) or between years (theta(ST) = 0.01). Most loci were consistent with a Hardy-Weinburg equilibrium and 82% of the genets between seasons from similar sampling localities constituted new genotypes. These results indicate that R. brevipes constitutes a randomly mating population with no genetic differentiation between locations or across successive fruiting seasons.
University of California, Berkeley, Department of Environmental Science, Policy and Management, Division of Ecosystem Science, 137 Mulford Hall #3114, Berkeley, CA 94720, USA. sbergemann@nature.berkeley.edu
Sarah E
SE
Bergemann
Greg W
GW
Douhan
Matteo
M
Garbelotto
Steven L
SL
Miller
D000363:Agaricales / Q000145:classification / Q000235:genetics*; D000483:Alleles; D000704:Analysis of Variance; D051456:Gene Flow; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D005843:Geography; D018895:Microsatellite Repeats / Q000235:genetics; D028223:Pinus / Q000382:microbiology*; D029963:Quercus / Q000382:microbiology*; D012098:Reproduction; D014197:Trees