The effects of IVF aspiration on the temperature, dissolved oxygen levels, and pH of follicular fluid.
OBJECTIVE
To investigate the effects of IVF aspiration on the temperature, pH, and dissolved oxygen of bovine follicular fluid.


METHODS
The temperature was monitored at various positions in an aspiration kit. Dissolved oxygen and pH were measured before and after aspiration.


RESULTS
The temperature of follicular fluid dropped by 7.7 +/- 1.3 degrees C upon aspiration. Dissolved oxygen levels rose by 5 +/- 2 vol.%. The pH increased by 0.04 +/- 0.01.


CONCLUSIONS
The temperature change was attributed mainly to evaporation of fluid in the collection tube. Changes in dissolved oxygen levels and pH were due to contact with air. Standard vacuum-based aspiration may induce changes in follicular fluid, which could be detrimental to oocyte health and affect attempts to correlate chemical characteristics with oocyte quality.
Food and Health, AgResearch Grasslands, 11008, Tennent Drive, Palmerston North 5301, New Zealand. gabe.redding@agresearch.co.nz
Gabe P
GP
Redding
John E
JE
Bronlund
Alan L
AL
Hart
D000818:Animals; D002417:Cattle; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000032:analysis; D013396:Suction; D013696:Temperature
D010100:Oxygen