[Characteristics of free Ca2+ distribution in cultured osteoclast-like cells].
OBJECTIVE
To study the spatial distribution of free Ca2+ in osteoclast-like cells cultured on glass.


METHODS
To detect the free Ca2+ in osteoclast-like cells, the images were analyzed with image software, using the laser scanning confocal microscope and fluorescent probe.


RESULTS
At 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM, the intensity of Ca2+ fluorescent signal in the central part was greater than that in the peripheral part and in the same section the signal was not distributed evenly.


CONCLUSIONS
The intensity of Ca2+ fluorescent signal is different among various organellae in osteoclast-like cell, which suggests the osteoclast-like cell modulate its own function through the spatial difference of free Ca2+ concentration.
Bao
Xiang-Jun
XJ
Shanghai Hospital of Oral Diseases Prevention, Shanghai 200001, China.
Liang
Xing
X
Chen
Ming
M
Wang
Hang
H
Song
Hong-Jie
HJ
Zhu
Bao-Min
BM
D000814:Aniline Compounds; D002118:Calcium; D002460:Cell Line; D002478:Cells, Cultured; D018613:Microscopy, Confocal; D010010:Osteoclasts; D014966:Xanthenes
D000814:Aniline Compounds; D014966:Xanthenes; C059715:Fluo-3; D002118:Calcium