ABCA2 is a marker of neural progenitors and neuronal subsets in the adult rodent brain.
The notion that the ATP-binding cassette transporter-A2 (ABCA2) may be involved in brain sterol homeostasis and is associated with early onset Alzheimer's disease led us to explore its neural expression. Our data support and extend the previous reports on ABCA2 expression by oligodendrocytes. They evidence that ABCA2 (i) is located in intracellular vesicles, identified in transfected cells as lysosome-related organelles only partially overlapping with classical endolysosomes; (ii) is a marker of neural progenitors as it is expressed in the subventricular zone of the lateral ventricle and the dentate gyrus of the hippocampal formation, sites of continual neurogenesis in the adult brain, and in nestin(+) cells differentiated in vitro from embryonic stem cells; (iii) persists, in the adult rodent brain, in a subset of GABAergic and glutamatergic neurons. Considering that the latter are targets of Alzheimer's lesions, these data provide a new rationale to explore the neuropathological consequences of ABCA2 functional dysregulations.
Centre d'Immunologie de Marseille Luminy INSERM CNRS, Université de la Méditerranée Marseille, France. chimini@ciml.univ-mrs.fr
Cyril
C
Broccardo
Vincent
V
Nieoullon
Rada
R
Amin
Frédérique
F
Masmejean
Sonia
S
Carta
Sara
S
Tassi
Matthieu
M
Pophillat
Anna
A
Rubartelli
Michel
M
Pierres
Geneviève
G
Rougon
André
A
Nieoullon
Genèvieve
G
Chazal
Giovanna
G
Chimini
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000166:cytology*; D051927:CD24 Antigen / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005455:Fluorescent Antibody Technique / Q000379:methods; D015870:Gene Expression / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism; D052119:Lysosomal-Associated Membrane Protein 2 / Q000378:metabolism; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000145:classification / Q000378:metabolism*; D051381:Rats; D013234:Stem Cells / Q000502:physiology*; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors; D014162:Transfection / Q000379:methods; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
C418186:ABCA2 protein, human; D018528:ATP-Binding Cassette Transporters; D015415:Biomarkers; D051927:CD24 Antigen; D005904:Glial Fibrillary Acidic Protein; D008164:Luminescent Proteins; D052118:Lysosomal-Associated Membrane Protein 1; D052119:Lysosomal-Associated Membrane Protein 2; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid