Senseless and Daughterless confer neuronal identity to epithelial cells in the Drosophila wing margin.
The basic helix-loop-helix (bHLH) proneural proteins Achaete and Scute cooperate with the class I bHLH protein Daughterless to specify the precursors of most sensory bristles in Drosophila. However, the mechanosensory bristles at the Drosophila wing margin have been reported to be unaffected by mutations that remove Achaete and Scute function. Indeed, the proneural gene(s) for these organs is not known. Here, we show that the zinc-finger transcription factor Senseless, together with Daughterless, plays the proneural role for the wing margin mechanosensory precursors, whereas Achaete and Scute are required for the survival of the mechanosensory neuron and support cells in these lineages. We provide evidence that Senseless and Daughterless physically interact and synergize in vivo and in transcription assays. Gain-of-function studies indicate that Senseless and Daughterless are sufficient to generate thoracic sensory organs (SOs) in the absence of achaete-scute gene complex function. However, analysis of senseless loss-of-function clones in the thorax implicates Senseless not in the primary SO precursor (pI) selection, but in the specification of pI progeny. Therefore, although Senseless and bHLH proneural proteins are employed during the development of all Drosophila bristles, they play fundamentally different roles in different subtypes of these organs. Our data indicate that transcription factors other than bHLH proteins can also perform the proneural function in the Drosophila peripheral nervous system.
Jafar-Nejad
Hamed
H
Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Tien
An-Chi
AC
Acar
Melih
M
Bellen
Hugo J
HJ
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D019070:Cell Lineage; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004625:Embryo, Nonmammalian; D004847:Epithelial Cells / Q000502:physiology*; D005786:Gene Expression Regulation; D017344:Genes, Insect; D007150:Immunohistochemistry; D009420:Nervous System / Q000196:embryology; D009474:Neurons / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014921:Wings, Animal / Q000166:cytology / Q000196:embryology*
D051792:Basic Helix-Loop-Helix Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C090080:Da protein, Drosophila; C413162:sens protein, Drosophila