[Isolation, culture, identification and biological characteristics of chicken skeletal muscle satellite cells].
Chicken skeletal muscle satellite cells were obtained by a two-step method of collagenase-I and trypsin digestion, followed by in vitro culture. They were further identified by specific gene expression assays. In addition, cell biological characteristics and myogenic genes expression were analyzed. Results showed that satellite cells from chicken skeletal muscle tissue expressed pax7, desmin, and possessed ability to proliferate and differentiate. The technical platform was established to study the mechanism of myogenic proliferation, differentiation and muscle regeneration.
Molecular and Cellular Developmental Biology Laboratory, Harbin Institute of Technology, Harbin 150001, China.
Yan
Y
Chen
Kun
K
Wang
Da-Hai
DH
Zhu
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D002645:Chickens; D017364:Collagenases / Q000378:metabolism; D003893:Desmin / Q000378:metabolism; D008297:Male; D051763:PAX7 Transcription Factor / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
D003893:Desmin; D051763:PAX7 Transcription Factor; D014357:Trypsin; D017364:Collagenases; C417249:collagenase 1