[Analysis on allele frequencies of 7 short tandem repeat loci of Kashing-Beck disease patients on].
OBJECTIVE
To analyze the allele frequencies of 7 short tandem repeat (STR) loci (D12S1718, D12S1675, D12S358, D12S367, D12S1638, D12S1646 and D12S1682) on chromosome 12 among Kashing-Beck disease (KBD) patients and the control population living in the KBD areas and non-KBD area.


METHODS
EDTA-blood specimens were collected from 102 unrelated individuals of Chinese Han population in Shaanxi province including 29 KBD patients,30 controls living in the KBD area and 43 living in the non-KBD area. DNA samples were extracted using the Wizard Genomic DNA purification kit (http://www. Promega. com) and were amplified by polymerase chain reaction (PCR) technique. The PCR products were analyzed by ABI 3100 Genetic Analyzer.


RESULTS
(1) In KBD patients group, the allele number for 7 STR loci were 4,7,7,8,5,5 and 7, the genotype number were 5,12,13,11,10,9 and 13; (2) In the control population living in KBD area, the allele number for 7 STR loci were 4,9,7,6,6,6 and 8,t he genotype number were 5,10,12,14,12,9 and 13;(3) In the control population living in the non-KBD area, the allele number for 7 STR loci were 7,9,7,7,5,8 and 11, the genotype number were 9,16, 17,16,12,15 and 20;(4) Compared with the allele frequencies among three groups, there were significant differences between KBD patients and the controls living in the KBD area (D12S367: P = 0.034; D12S1638: P = 0.041) and the controls living in the non-KBD area (D12S367: P = 0. 029; D12S1638: P= 0 .028) in the D12S367 and D12S1638 loci; (5) There were significant differences among KBD patients (P = 0.036), controls living in the KBD area (P = 0.039) and controls living in the non-KBD area in the D12S1646.


CONCLUSIONS
There was significant difference between KBD patients and the controls in the D12S367 and D12S1638 loci.
The State Key Laboratory of Environment and Disease Gene, Institute of Endemic Disease, Xi 'an Jiaotong University, Xi 'an 710061, China.
Long-li
LL
Kang
Xiong
X
Guo
Hong
H
Zuo
Zhi-guang
ZG
Ping
Bao-di
BD
Zhang
Jianghua
J
Lai
Dong
D
Geng
D000328:Adult; D016022:Case-Control Studies; D002648:Child; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056426:Genetic Loci / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007592:Joint Diseases / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*