Targeted expression of Ip3 sponge and Ip3 dsRNA impaires sugar taste sensation in Drosophila.
We evaluated the role of IP(3) in sugar taste reception in Drosophila melanogaster by inactivating the IP(3) signaling using genetic tools. We used the "IP(3) sponge," composed of the modified ligand-binding domain from the mouse IP(3) receptor, which was designed to absorb IP(3) in competition with native IP(3) receptors. Another tool was a transgene that generates double-stranded RNA against IP(3) receptor mRNA. Both inhibitors diminished the sensitivity of flies to trehalose and sucrose, as estimated by behavioral assays and electrophysiological recordings from the sugar receptor cells. The result indicates that IP(3) signaling is indispensable for sugar reception in Drosophila.
Waseda University, School of Science and Engineering, Nishi-Tokyo, Japan.
Kazue
K
Usui-Aoki
Ken
K
Matsumoto
Masayuki
M
Koganezawa
Sou
S
Kohatsu
Kunio
K
Isono
Hiroshi
H
Matsubayashi
Masa-Toshi
MT
Yamamoto
Ryu
R
Ueda
Kuniaki
K
Takahashi
Kaoru
K
Saigo
Katsuhiko
K
Mikoshiba
Daisuke
D
Yamamoto
D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal / Q000502:physiology; D004187:Disaccharides / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D004594:Electrophysiology; D051717:Fushi Tarazu Transcription Factors / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D015544:Inositol 1,4,5-Trisphosphate / Q000235:genetics*; D008024:Ligands; D051379:Mice; D012330:RNA, Double-Stranded / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D013395:Sucrose / Q000378:metabolism; D013649:Taste / Q000235:genetics*; D014199:Trehalose / Q000378:metabolism
D004187:Disaccharides; D029721:Drosophila Proteins; D051717:Fushi Tarazu Transcription Factors; C080545:GAL4-ftzQ protein, Drosophila; D008024:Ligands; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C405956:gustatory receptor, Drosophila; D013395:Sucrose; D015544:Inositol 1,4,5-Trisphosphate; D014199:Trehalose