Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment.
The immunophenotypic analysis of ex vivo-expanded mesenchymal stromal cells (MSC) has so far been confined to single or dual staining analysis in normal subjects. In this study, using a four-color cytofluorimetric protocol, we demonstrated that cultured MSC derived from the bone marrow of patients with hematologic malignancies showed alterations in the expression of CD105, CD90, CD184, and HLA-DR molecules. The decrease in the percentage of CD105+ and CD90+ MSC correlated with an increased bone marrow angiogenesis. This paper provides evidence that multiparametric flow cytometry is essential for the establishment of a standardized protocol to identify various MSCs subsets and aberrant phenotypes.
Campioni
Diana
D
Section of Hematology, University Hospital, Ferrara, Italy.
Moretti
Sabrina
S
Ferrari
Luisa
L
Punturieri
Marina
M
Castoldi
Gian Luigi
GL
Lanza
Francesco
F
D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000276:immunology*; D002478:Cells, Cultured; D004777:Environment; D006402:Hematologic Diseases / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D008875:Middle Aged; D017154:Stromal Cells / Q000276:immunology