Biomimicking extracellular matrix: cell adhesive RGD peptide modified electrospun poly(D,L-lactic-co-glycolic acid) nanofiber mesh.
A cell adhesive peptide, Arg-Gly-Asp (RGD), was immobilized onto the surface of electrospun poly(D,L-lactic-co-glycolic acid) PLGA nanofiber mesh in an attempt to mimic an extracellular matrix structure. A blend mixture of PLGA and PLGA-b-PEG-NH(2) di-block copolymer dissolved in a 1:1 volume mixture of dimethylformamide and tetrahydrofuran was electrospun to produce a nanofiber mesh with functional primary amino groups on the surface. Various electrospinning parameters, such as polymer concentration and the blend ratio, were optimized to produce a nanofiber mesh with desirable morphology, surface characteristics, and fiber diameter. A cell adhesive peptide, GRGDY, was covalently grafted onto the aminated surface of the electrospun mesh under a hydrating condition. The amounts of surface primary amino groups and grafted RGD peptides were quantitatively determined. Cell attachment, spreading, and proliferation were greatly enhanced in the RGD modified electrospun PLGA nanofiber mesh compared with that of the unmodified one.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
Taek Gyoung
TG
Kim
Tae Gwan
TG
Park
D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D004126:Dimethylformamide / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry*; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D005663:Furans / Q000737:chemistry; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D008970:Molecular Weight; D041681:NIH 3T3 Cells; D036103:Nanotechnology; D009842:Oligopeptides / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties; D014783:Viscosity
D005663:Furans; D009842:Oligopeptides; D011091:Polyesters; D011108:Polymers; D012997:Solvents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C018674:tetrahydrofuran; C047981:arginyl-glycyl-aspartic acid; D004126:Dimethylformamide