[Multiple isoforms of cyclophilin A associated with human immunodeficiency virus type 1].
It is well-known that a peptidyl-prolyl cis-trans isomerase cyclophilin A (CyPA) is incorporated into Human immunodeficiency virus type 1 (HIV-1) particle. The proteome analysis of the purified HIV-1 strain LAV-1 (HIV-1(LAV-1)) reveals that three isoforms of CyPA with an isoelectric point (pI) of 6.00, 6.40, and 6.53 are inside the viral membrane and another isoform with a pI of 6.88 is outside the viral membrane; and that the CyPA isoform with a pI of 6.53 is N-acetylated. The mechanisms that permit the redistribution of CyPA with a pI of 6.88 on the viral surface have not yet been clarified, but it penetrates the viral membrane after budding.
Department of Pharmaceutical Biochemistry, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Japan. misumi@gpo.kumamoto-u.ac.jp
Shogo
S
Misumi
D000818:Animals; D021984:Cyclophilin A / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007525:Isoelectric Focusing; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D020543:Proteome; D014763:Viral Matrix Proteins; D019065:Virus Assembly / Q000235:genetics
D020033:Protein Isoforms; D020543:Proteome; D014763:Viral Matrix Proteins; D021984:Cyclophilin A