Environmental proteomics and metallomics.
Monitoring environmental pollution using biomarkers requires detailed knowledge about the markers, and many only allow a partial assessment of pollution. New proteomic methods (environmental proteomics) can identify proteins that, after validation, might be useful as alternative biomarkers, although this approach also has its limitations, derived mainly from their application to non-model organisms. Initial studies using environmental proteomics were carried out in animals exposed to model pollutants, and led to the concept of protein expression signatures. Experiments have been carried out in model organisms (yeast, Arabidopsis, rat cells, or mice) exposed to model contaminants. Over the last few years, proteomics has been applied to organisms from ecosystems with different pollution levels, forming the basis of an environmental branch in proteomics. Another focus is connected with the presence of metals bound to biomolecules, which adds an additional dimension to metal-biomolecule and metalloprotein characterization - the field of metallomics. The metallomic approach considers the metallome: a whole individual metal or metalloid species within a cell or tissue. A metallomic analytical approach (MAA) is proposed as a new tool to study and identify metalloproteins.
López-Barea
Juan
J
Department of Biochemistry and Molecular Biology, University of Córdoba, Córdoba, Spain. bb1lobaj@uco.es
Gómez-Ariza
José Luis
JL
D000818:Animals; D004777:Environment; D004785:Environmental Pollutants / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008667:Metalloproteins / Q000737:chemistry / Q000378:metabolism; D008670:Metals / Q000378:metabolism*; D020543:Proteome / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics
D004785:Environmental Pollutants; D008667:Metalloproteins; D008670:Metals; D020543:Proteome