Human arylamine N-acetyltransferase 2 polymorphism and susceptibility to allergic contact dermatitis.
BACKGROUND
N-acetyltransferase 2 (NAT2) polymorphism may be involved in the pathogenesis of allergic contact dermatitis.


OBJECTIVE
The present study was designed to evaluate whether acetylation polymorphism plays a role in the susceptibility to p-Phenylenediamine (PPD) sensitization.


METHODS
The frequencies of seven NAT2 point mutations, namely G191A, C282T, T341C, C481T, G590A, A803G, and G857A, and genotypes were determined by PCR/RFLP in a total of 70 patients with allergic contact dermatitis to PPD and 100 control subjects with no history of allergy, atopy, lung disease, diabetes mellitus and cancer.


RESULTS
Genotypes coding rapid acetylation were detected in 52.9% and 37.0% of patients with contact dermatitis and control subjects, respectively (P = 0.04). The frequency of the NAT2*4 allele and NAT2*4/*4 genotype, coding for rapid acetylation, were also significantly higher in the contact dermatitis patients than in the control subjects (P = 0.003).


CONCLUSIONS
Our results suggest an association between rapid acetylation polymorphism and susceptibility to PPD sensitization.
Department of Pharmacology, Faculty of Medicine, Gaziantep University, Turkey.
Muradiye
M
Nacak
Zulal
Z
Erbagci
A Sukru
AS
Aynacioglu
D000107:Acetylation; D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D004396:Coloring Agents / Q000009:adverse effects* / Q000378:metabolism; D017449:Dermatitis, Allergic Contact / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010655:Phenylenediamines / Q000009:adverse effects* / Q000378:metabolism; D017354:Point Mutation; D011110:Polymorphism, Genetic
D004396:Coloring Agents; D010655:Phenylenediamines; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human; C029728:4-phenylenediamine