Bioconversion of 7-Aminocephalosporanic Acid by Intact Rhodotorula glutinis Cells.
When 7-aminocephalosporanic acid (7-ACA) was used as a single carbon source in the enrichment culture medium for screening 7-ACA-degrading microorganisms, pink yeast colonies appeared frequently, and these were identified as Rhodotorula glutinis. These intact R. glutinis cells converted (i) 7-ACA to deacetyl-7-ACA (7-ADACA) and (ii) monochloroacetyl-7-ACA to monochloroacetyl-7-ADACA at sufficiently high levels to be of commercial interest. Acetylation of 7-ADACA to 7-ACA, the reverse reaction of hydrolysis in an organic medium with methyl acetate as an acetyl donor, was also demonstrated.
Sakai
Y
Y
Abe
T
T
Ohbayashi
Y
Y
Isaka
K
K
Yamamoto
K
K
Tani
Y
Y
Kato
N
N