Genomic compositions and phylogenetic analysis of Shigella boydii subgroup.
Comparative Genomic Hybridization (CGH) microarray analysis was used to compare the genomic compositions of all eighteen Shigella boydii serotype representative strains. The results indicated the genomic "backbone" of this subgroup contained 2552 ORFs homologous to nonpathogenic E. coli K12. Compared with the genome of K12199 ORFs were found to be absent in all S. boydii serotype representatives, including mainly outer membrane protein genes and O-antigen biosynthesis genes. Yet the specific ORFs of S. boydii subgroup contained basically bacteriophage genes and the function unknown (FUN) genes. Some iron metabolism, transport and type II secretion system related genes were found in most representative strains. According to the CGH phylogenetic analysis, the eighteen S. boydii serotype representatives were divided into four groups, in which serotype C13 strain was remarkably distinguished from the other serotype strains. This grouping result corresponded to the distribution of some metabolism related genes. Furthermore, the analysis of genome backbone genes, specific genes, and the phylogenetic trees allowed us to discover the evolution laws of S. boydii and to find out important clues to pathogenesis research, vaccination and the therapeutic medicine development.
State Key Laboratory for Molecular Virology and Genetic Engineering, Beijing 100176, China.
Jing
J
Wang
Xiaobing
X
Zhang
Junping
J
Peng
E
E
Yang
Wen
W
Bin
Jian
J
Yang
Jie
J
Dong
Lilian
L
Sun
Xingye
X
Xu
Qi
Q
Jin
D017353:Gene Deletion; D016680:Genome, Bacterial; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D010802:Phylogeny; D012761:Shigella boydii / Q000235:genetics*