Whey feeding suppresses the measurement of oxidative stress in experimental burn injury.
OBJECTIVE
Burns cause thermal injury to local tissue and trigger systemic acute inflammatory processes, which may lead to multiple distant organ dysfunction. We investigated the protective effect of dietary whey supplementation on distant organs in a rat model.


METHODS
Forty-eight rats were divided into six groups of eight: groups 1 and 2 were the controls, fed a standard diet and a whey-supplemented diet, respectively; groups 3 and 4 were fed a standard diet and subjected to burn injury; and groups 5 and 6 were fed a whey-supplemented diet and subjected to burn injury. We measured the oxidative stress variables, as well as glutathione in the liver and kidney, and histologically examined skin samples obtained 4 h (groups 3 and 5) and 72 h (groups 4 and 6) after burn injury.


RESULTS
Glutathione (GSH) levels remained the same in the liver but were slightly elevated in the kidneys after burn injury in the rats fed a standard diet. Whey supplementation caused a significant increase in hepatic GSH levels 4 h after burn injury. Moreover, there was a significant rebound effect in the liver and kidney GSH levels after 72 h and whey supplementation potentiated this effect. Hepatic and renal lipid peroxide levels were also increased 4 h after burn injury in the rats fed a standard diet. Whey supplementation significantly suppressed the burn-induced increase in hepatic and renal lipid peroxide levels. Histological examination revealed that although whey supplementation resulted in decreased subepidermal inflammation, the indicators of wound healing and collagen deposition were not improved.


CONCLUSIONS
Whey pretreatment suppressed hepatic and renal oxidative stress measurements after experimental burn injury.
Oner
Osman Z
OZ
Department of General Surgery, Marmara University, Tophanelioğlu cad., Altunizede, Istanbul, Turkey.
Oğünç
Ayliz Velioğlu
AV
Cingi
Asim
A
Uyar
Süheyla Bozkurt
SB
Yalçin
A Süha
AS
Aktan
A Ozdemir
AO
D000818:Animals; D002056:Burns / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D003611:Dairy Products; D019587:Dietary Supplements; D005978:Glutathione; D007668:Kidney; D008099:Liver; D008894:Milk Proteins / Q000494:pharmacology*; D023421:Models, Animal; D009752:Nutritional Status; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D000067816:Whey Proteins
D008894:Milk Proteins; D000067816:Whey Proteins; D005978:Glutathione