Association between apolipoprotein E polymorphism and coronary artery disease in the Kermanshah population in Iran.
OBJECTIVE
Coronary artery disease (CAD) is the major cause of death in developing countries, such as Iran. The apolipoprotein E gene (APOE) is considered an important genetic determinant of CAD. In this study, the relationship between APOE polymorphism with lipid variation in CAD patients in Kermanshah, Iran was investigated.


METHODS
This case-control study consisted of 115 CAD patients who angiographically had at least 30% stenosis and 135 unrelated controls. APOE polymorphism was detected by PCR-RFLP and serum lipid level was measured enzymatically.


RESULTS
The APOE-epsilon4 and -epsilon2 allele frequencies were significantly higher in the CAD patients than in the control group (P < 0.001). The CAD patients with epsilon3/epsilon4 genotype had also higher TC (P < 0.001) and LDL-C (P < 0.01) and lower HDL-C (P < 0.03) levels than that of the control group.


CONCLUSIONS
APOE-epsilon4 allele is a risk factor for CAD, so that carriers of this allele with high levels of LDL-C may be susceptible to CAD and myocardial infarction.
Kharrazi
H
H
Department of Medical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Vaisi Raygani
A
A
Sabokroh
A R
AR
Pourmotabbed
T
T
D001057:Apolipoproteins E / Q000235:genetics*; D001483:Base Sequence; D003324:Coronary Artery Disease / Q000453:epidemiology / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D005828:Genetics, Population; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D001057:Apolipoproteins E; D017931:DNA Primers