The total antioxidant status in the brain after ethanol or 4-methylpyrazole administration to rats intoxicated with methanol.
In the study, the potential interaction of methanol/ethanol and methanol/4-methylpyrazole within the scope of the total antioxidant status (TAS) in rat brain was investigated. The experiment was carried out on rats exposed to N2O/O2 mixture (1:1 v/v) which enable to obtain similar response of rat organism to methanol poisoning as that observed in humans. The rats were exposed to N2O/O2 mixture 4h before the administration of methanol or saline. The exposure to N2O/O2 was continued throughout the experiment. Methanol was administered orally at a dose of 3.0 g/kg, then, after next 4 h ethanol (0.5 or 1g/kg) or 4-methylpyrazole (4-MP) (15.0 or 50.0 mg/kg.) was administered by i.p. injection. TAS in rat brain homogenates was assayed spectrophotometrically at lambda=600. The mean concentration of TAS in rats exposed to N2O/O2 mixture did not differ from that determined for control rats. A slight decrease in TAS concentration was found in all groups of rats intoxicated with methanol and ethanol or 4-MP and methanol alone. There was no interaction between methanol and 4-MP. However, a significant decrease in TAS concentration was observed in rats following the ingestion of methanol and than after the administration of 1.0 g/kg of ethanol in comparison to rats receiving methanol alone (p<0.05). This phenomenon suggests an adverse interaction of methanol/ethanol within scope of the brain antioxidant status equilibrium.
Department of Clinical Pathomorphology, Medical University of Lublin, 8 Jaczewskiego Str., PL-20-093 Lublin, Poland. ave123@wp.pl
Jaroslaw
J
Dudka
D000280:Administration, Inhalation; D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D000431:Ethanol / Q000633:toxicity*; D000077604:Fomepizole; D007274:Injections, Intraperitoneal; D000432:Methanol / Q000633:toxicity*; D009609:Nitrous Oxide / Q000008:administration & dosage; D010100:Oxygen / Q000008:administration & dosage; D011720:Pyrazoles / Q000633:toxicity*; D051381:Rats; D012997:Solvents / Q000633:toxicity*
D000975:Antioxidants; D011720:Pyrazoles; D012997:Solvents; D000431:Ethanol; D000077604:Fomepizole; D009609:Nitrous Oxide; D010100:Oxygen; D000432:Methanol