Proteins unique to intraphagosomally grown Mycobacterium tuberculosis.
Pathogenic mycobacteria persist and replicate within phagosomes of host phagocytes by inhibiting phagosome maturation at an early endosome stage. The molecular basis for this behavior is not understood. To identify proteins of Mycobacterium tuberculosis unique to the intraphagosomal phase, mycobacteria were purified from phagosomes of infected murine bone marrow-derived macrophages and analyzed by high-resolution 2-DE and MS. Protein patterns of intraphagosomally grown M. tuberculosis were compared with those of broth-cultured mycobacteria. The analysis revealed 11 mycobacterial proteins exclusively detected in intraphagosomal mycobacteria. Some of these proteins are involved in metabolism and cell envelope synthesis, such as the lipid carrier protein Rv1627c, and the conserved hypothetical protein Rv1130 that shows homology to a virulence-associated protein of Legionella pneumophila. The relevance of these proteins as factors enabling intracellular survival of M. tuberculosis is being discussed.
Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany. mattow@mpiib-berlin.mpg.de
Jens
J
Mattow
Frank
F
Siejak
Kristine
K
Hagens
Dörte
D
Becher
Dirk
D
Albrecht
Alexander
A
Krah
Frank
F
Schmidt
Peter R
PR
Jungblut
Stefan H E
SH
Kaufmann
Ulrich E
UE
Schaible
D001426:Bacterial Proteins / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D008264:Macrophages / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D010588:Phagosomes / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D016622:Silver Staining / Q000379:methods; D037521:Virulence Factors / Q000378:metabolism
D001426:Bacterial Proteins; D037521:Virulence Factors