[Preparation of hematoporphyrin-herceptin photoimmunoconjugate for photoimmunotherapy].
OBJECTIVE
To prepare photoimmunoconjugate of hematoporphyrin (HP) and herceptin, and study its killing and apoptosis-inducing effect on tumor cells BT-474.


METHODS
HP-herceptin photoimmunoconjugate was synthesized with EDCI as the condensator. After exposure of the cells to 630 nm laser, the killing effect of the conjugate and cell apoptosis were evaluated by MTT assay and flow cytometry.


RESULTS
Compared with free HP at equivalent dose, the immune reactivity, killing effect and the apoptosis-inducing effect of HP-herceptin immunoconjugate on BT-474 cells was enhanced (P<0.05).


CONCLUSIONS
The killing effect of HP-herceptin immunoconjugate is stronger than free HP on BT-474 cells.
Center of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Lu
L
Chen
Rong-cheng
RC
Luo
Li-bo
LB
Li
Xiao
X
Yan
Xue-mei
XM
Ding
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000494:pharmacology; D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004339:Drug Compounding / Q000379:methods; D005434:Flow Cytometry; D006414:Hematoporphyrin Photoradiation / Q000379:methods; D006415:Hematoporphyrins / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry* / Q000494:pharmacology; D007167:Immunotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology; D000068878:Trastuzumab
D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents; D006415:Hematoporphyrins; D018796:Immunoconjugates; D017319:Photosensitizing Agents; D000068878:Trastuzumab