[Protective effects of saponines of stem and leaf of Panax notoginseng on acute myocardial ischemia in anaesthetic dogs].
OBJECTIVE
To study the protective effects of saponines of stem and leaf of Panax notoginseng (PNSSL) on acute myocardial ischemia in anaesthetic dogs.


METHODS
The acute ischemia models were made by ligation of left anterior descending (LAD) artery. The myocardial blood flow (MBF) was determined by ultrasonic doppler. The experiments adopted epicardiogram mapping to measure the scope and degree of myocardial ischemia, quantitative histologic assay (nitroblue tetrazolium, N-BT stain) to determine the size of myocardial infarction. And the endothelin (ET) and thromboxane B2 (TXB2) were measured by radioimmunological assay.


RESULTS
PNSSL was showed to obviously alleviate the degree of myocardial ischemia (sigma-ST) and narrow the ischemic area indicated by N-BT staining. In addition, PNSSL could increase the MBF of ischemia section. And the treatment could inhibit the ET and TXB2 release induced by ischemia and infarction.


CONCLUSIONS
PNSSL demonstrated to attenuate the damage subjected to myocardial ischemia and infarction, which may be due to its function of inhibiting the ET and TXA2 release, increasing the MBF, and then improving the damaged cardiac function.
Fu
Jian-hua
JH
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing.
Li
Xin-zhi
XZ
Shang
Xiao-hong
XH
Liu
Jian-xun
JX
D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects; D004285:Dogs; D016232:Endothelins / Q000097:blood; D005260:Female; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000503:physiopathology; D005894:Panax / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D011897:Random Allocation; D013929:Thromboxane B2 / Q000097:blood
D016232:Endothelins; D036145:Ginsenosides; D020011:Protective Agents; D013929:Thromboxane B2