Bovine T cell receptor gamma variable and constant genes: combinatorial usage by circulating gammadelta T cells.
Studies here describe expression and sequence of several new bovine T cell receptor gamma (TRG) genes to yield a total of 11 TRG variable (TRGV) genes (in eight subgroups) and six TRG constant (TRGC) genes. Publicly available genomic sequences were annotated to show their placement. Homologous TRG genes in cattle and sheep were assigned, using four accepted criteria. New genes described here include the bovine TRGC6, TRGV2, and TRGV4, homologues of ovine TRGC4, TRGV2, and TRGV4, respectively. The bovine Vgamma7 and BTGV1 clones (previously TRGV4 and TRGV2, respectively) were reassigned to new subgroups TRGV7 and TRGV8, respectively, with approval by the IMGT Nomenclature Committee. Three TRGV subgroups (TRGV5, TRGV6, and TRGV8) were further designated as TRGV5-1 and TRGV5-2, TRGV6-1 and TRGV6-2, and TRGV8-1 and TRGV8-2 because each subgroup is comprised of two mapped genes. The complete sequence of bovine TRGC5 is also reported, for which a limited number of nucleotides was previously available, and shown to be most closely related to ovine TRGC5. Analysis of circulating gammadelta T cells revealed that rearrangement of TRGV genes with TRGC genes is largely dictated by their proximity within one of the six genomic V-J-C cassettes, with all TRG genes expressed by bovine peripheral blood gammadelta T cells. Cattle are useful models for gammadelta T cell biology because they have gammadelta T cells that respond to isopentenylpyrophosphate (IPP) antigens, while mice do not, and some bovine TRGV genes cluster closely with human genes.
Herzig
Carolyn
C
Paige Laboratory, Department of Veterinary and Animal Sciences and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, USA. cbaldwin@vasci.umass.edu
Blumerman
Seth
S
Lefranc
Marie-Paule
MP
Baldwin
Cynthia
C
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000276:immunology*; D002874:Chromosome Mapping; D015870:Gene Expression; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D019675:Genes, T-Cell Receptor gamma / Q000235:genetics*; D016678:Genome; D045782:Hemiterpenes / Q000494:pharmacology; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010802:Phylogeny; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000145:classification / Q000235:genetics*; D012756:Sheep / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D045782:Hemiterpenes; D007127:Immunoglobulin Constant Regions; D007135:Immunoglobulin Variable Region; D009943:Organophosphorus Compounds; D016692:Receptors, Antigen, T-Cell, gamma-delta; C004809:isopentenyl pyrophosphate