Glucose oxidase/colloidal gold nanoparticles immobilized in Nafion film on glassy carbon electrode: Direct electron transfer and electrocatalysis.
The direct electron transfer of glucose oxidase (GOD) was achieved based on the immobilization of GOD/colloidal gold nanoparticles on a glassy carbon electrode by a Nafion film. The immobilized GOD displayed a pair of well-defined and nearly reversible redox peaks with a formal potential (Eo ') of -0.434 V in 0.1 M pH 7.0 phosphate buffer solution and the response showed a surface-controlled electrode process. The dependence of Eo ' on solution pH indicated that the direct electron transfer reaction of GOD was a two-electron-transfer coupled with a two-proton-transfer reaction process. The experimental results also demonstrated that the immobilized GOD retained its electrocatalytic activity for the oxidation of glucose. So the resulting modified electrode can be used as a biosensor for detecting glucose.
Zhao
Shuang
S
College of Chemistry, Jilin University, Changchun, 130012, PR China.
Zhang
Kai
K
Bai
Yu
Y
Yang
Weiwei
W
Sun
Changqing
C
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon; D002384:Catalysis; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry; D005465:Fluorocarbon Polymers / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D017979:Gold Colloid / Q000737:chemistry*; D008839:Microelectrodes; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size
D004800:Enzymes, Immobilized; D005465:Fluorocarbon Polymers; D017979:Gold Colloid; C040402:perfluorosulfonic acid; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose