Chromosomal and genetic imbalances in synovial sarcoma detected by conventional and microarray comparative genomic hybridization.
OBJECTIVE
To analyze the relationship between chromosomal instabilities and clinicopathological factors in synovial sarcoma (SS).


METHODS
Twenty-two fresh-frozen SS were analyzed by metaphase comparative genomic hybridization (CGH). Additional microarray CGH was performed in 13 cases.


RESULTS
Fourteen patients with SYT-SSX1 rearrangements and nine patients with biphasic tumor subtypes had better prognosis than the eight patients with SYT-SSX2 rearrangements and 13 patients with monophasic subtypes, respectively. Gains (average 3.0) were more frequent than losses (average 1.0). Frequent gains were identified on chromosomal regions 2, 6q, 7, 8q, 12, 17q, 18q, and 21q, whereas frequent losses were over-lapped on chromosomes 1p31-p35, 3p, 6q, 16, and 17p. High-level gains were observed on chromosomes 1q21-q31, 7, 8, 12, 17q, 18q, and 21q. Thirteen monophasic and nine biphasic tumors had an average of 5.1 and 2.8 aberrations, respectively. Patients with tumors harboring numerous aberrations (>or=3) had a worse clinical course. Microarray CGH more specifically detected genetic imbalances including gains in MDM2, MSH2, KCNK12, DCC, CDK2, ERBB3, SAS, and CDK4 and losses in HRAS, RASSF1, and CCND1. Gain of SAS was an important prognostic factor of SS.


CONCLUSIONS
We have identified several factors influencing the prognosis of SS patients by metaphase and microarray CGH.
Department of Orthopaedic Surgery, Science of Functional Recovery and Reconstruction, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, 700-8558 Okayama, Japan.
Yasuko
Y
Nakagawa
Kunihiko
K
Numoto
Aki
A
Yoshida
Toshiyuki
T
Kunisada
Hidenori
H
Ohata
Ken
K
Takeda
Daniel
D
Wai
Christopher
C
Poremba
Toshifumi
T
Ozaki
D000293:Adolescent; D000328:Adult; D043171:Chromosomal Instability; D005260:Female; D006801:Humans; D008297:Male; D008677:Metaphase; D046228:Microarray Analysis; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods; D011237:Predictive Value of Tests; D011379:Prognosis; D013584:Sarcoma, Synovial / Q000235:genetics* / Q000473:pathology*; D016019:Survival Analysis