Dominant tolerance to kidney allografts induced by anti-donor MHC class II antibodies: cooperation between T and non-T CD103+ cells.
Allograft acceptance can be induced in the rat by pretransplant infusion of donor blood or spleen cells. Although promoting long-term acceptance, this treatment is also associated with chronic rejection. In this study, we show that a single administration of anti-donor MHC class II alloimmune serum on the day of transplantation results in indefinite survival of a MHC-mismatched kidney graft. Long-term recipients accept a donor-type skin graft and display no histological evidence of chronic rejection. The kidney grafts of tolerant animals display an accumulation of TCR Cbeta, FoxP3, and IDO transcripts. Moreover, as compared with syngeneic recipients, tolerant recipients harbor a large infiltrate of MHC class II(+) cells and CD103(+) cells. In vitro, splenocytes from tolerant recipients exhibit decreased donor-specific proliferation, which is restored by depletion of non-T cells and partially restored by the blockade of IDO. Finally, splenocytes from tolerant recipients, but not purified T cell splenocytes, transfer donor-specific infectious tolerance without chronic rejection, after infusion into naive recipients, over two generations. However, splenocytes depleted of T cells or splenocytes depleted of CD103(+) cells fail to transfer tolerance. Collectively, these data show that a single administration of anti-donor MHC class II alloimmune serum induces a tolerant state characterized by an infiltration of the kidney graft by regulatory T cells and CD103(+) cells. These data also show that the transfer of tolerance requires the presence of both T cells and CD103(+) dendritic cells. The precise mechanism of cooperation of these two cell subsets remains to be defined.
Degauque
Nicolas
N
Institut National de la Santé et de la Recherche Médicale-Université de Nantes, Unité Mixte de Recherche 643, France.
Lair
David
D
Dupont
Alexandre
A
Moreau
Anne
A
Roussey
Gwénaelle
G
Moizant
Frédérique
F
Hubert
François Xavier
FX
Louvet
Cédric
C
Hill
Marcelo
M
Haspot
Fabienne
F
Josien
Régis
R
Usal
Claire
C
Vanhove
Bernard
B
Soulillou
Jean Paul
JP
Brouard
Sophie
S
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation; D006085:Graft Survival / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007108:Immune Tolerance / Q000276:immunology*; D039001:Integrin alpha Chains / Q000276:immunology / Q000378:metabolism*; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D010641:Phenotype; D051381:Rats; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D016038:Skin Transplantation / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D015996:Survival Rate; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D014019:Tissue Donors; D014184:Transplantation, Homologous / Q000276:immunology
D000906:Antibodies; D015703:Antigens, CD; D000949:Histocompatibility Antigens Class II; D039001:Integrin alpha Chains; D016693:Receptors, Antigen, T-Cell, alpha-beta; C091367:alpha E integrins