Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase.
This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1(a) gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.
Song
Jae Seok
JS
Department of Biological Sciences, Myongji University, San 38-2 Namdong, Yongin, Gyeonggido 449-728, Republic of Korea.
Lee
Jung Hun
JH
Lee
Jung-Hyun
JH
Jeong
Byeong Chul
BC
Lee
Won-Keun
WK
Lee
Sang Hee
SH
D003666:Decontamination / Q000379:methods*; D003851:Deoxyribonucleases / Q000378:metabolism; D004866:Equipment Contamination; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D019914:Taq Polymerase / Q000235:genetics* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics*
D011933:Reagent Kits, Diagnostic; D019914:Taq Polymerase; D003851:Deoxyribonucleases; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1