Altered allergic cytokine and antibody response in mice treated with Bisphenol A.
The objective of this study was to elucidate if Bisphenol A (BPA) administration modulates T helper (Th) cell component of immune responses in a mouse challenged with ovalbumin (OVA), a major food antigen. BALB/c mice, (6 weeks old, female) were orally given either OVA (OVA-fed) or water (Water-fed), immunized intraperitoneally with OVA and injected with either BPA in corn oil or the vehicle alone. After subsequent 2nd immunization, serum titers of total IgE, OVA-specific IgE, IgG, IgG1 IgG2a and ability of their splenocytes for production of interferon (IFN) -gamma, interleukin (IL) -4 and IL-12 were examined by ELISA. Lymphocyte proliferation assay against concanavalin A (Con A) or OVA was also performed for 3H-Thymidine incorporation. In Water-fed groups, treatment with BPA resulted in lower titers of total IgE (P<0.01) and higher levels IgG2a (P<0.05) followed by a higher IFN-gamma (P<0.05) and IL-12 (P<0.05) with an intact IL-4. When OVA-fed groups were examined, the compound did not change production of total and OVA-specific IgE and -IgG2a but resulted in lower production of IFN-gamma (P<0.05). Also, BPA resulted in impaired lymphocyte proliferation to Con A in Water-fed groups (P<0.05) but not in tolerated animals. The findings indicate that BPA results in augmentation of Th1 immune responses but no significant effect on an established tolerance to OVA.
Alizadeh
Mohammad
M
Department of Preventive Environment and Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan.
Ota
Fusao
F
Hosoi
Kazuo
K
Kato
Makoto
M
Sakai
Tohru
T
Satter
Mohammed A
MA
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001559:Benzhydryl Compounds; D016207:Cytokines / Q000096:biosynthesis*; D052244:Endocrine Disruptors / Q000633:toxicity*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010636:Phenols / Q000633:toxicity*; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D016207:Cytokines; D052244:Endocrine Disruptors; D010047:Ovalbumin; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds