Optimization of medium and cultivation conditions for alkaline protease production by the marine yeast Aureobasidium pullulans.
A yeast strain, Aureobasidium pullulans, which could produce the high yield of protease was isolated from sediment of saltern in Qingdao, China. Maximum production of enzyme (623.1 U/mg protein; 7.2 U/ml) was obtained in a medium containing 2.5 g soluble starch and 2.0 g NaNO(3), 100ml seawater, initial pH 6.0, after fermentation at 24.5 degrees C for 30 h. The protease had the highest activity at pH 9.0 and 45 degrees C.
UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China. zhenming@sdu.edu.cn
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D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development; D001426:Bacterial Proteins / Q000378:metabolism*; D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D010450:Endopeptidases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
D001426:Bacterial Proteins; D003470:Culture Media; D002244:Carbon; D010450:Endopeptidases; C087822:alkaline protease; D009584:Nitrogen