[Screening and identification of therapeutic effect evaluation antigens of angiostrongyliasis].
OBJECTIVE
To identify antigens which may help evaluate the therapeutic effect of angiostrongyliasis from adult worm antigen of Angiostrongylus cantonensis.


METHODS
The adult worm antigens of A. cantonensis were analyzed by Western blotting with the sera of rats infected with A. cantonensis before and after treatment. The sera of rats were tested by enzyme-linked immunosorbent assay (ELISA).


RESULTS
The antigens with relative molecular mass between 38,000 and 78,000 reacted not only with the sera of rats before treatment, but also with that after treatment. The antigens with M(r) between 190,000 and 17,000 reacted with the sera of rats before treatment but not with that after treatment; those with M(r) between 32,000 and 24,000 antigens strongly reacted with the former, but the reaction became much weakened with the latter. The AC32-IgG antibody appeared earlier than the AC-IgG, and disappeared rapidly after treatment. Six of the 10 treated rats became negative for AC-IgG as found by ELISA.


CONCLUSIONS
The antigens of adult worm antigen of A. cantonensis with M(r) of 190,000, 32,000, 24,000, 17,000 and 16,000 may serve as candidate antigens for therapeutic effect evaluation of angiostrongyliasis.
Department of Pathogenic Biology, Southern Medical University, Guangzhou 510515, China.
Xing-cun
XC
Zhao
Jin-bao
JB
Gu
Hua
H
Li
Min
M
Liu
Hao-xian
HX
Shen
Xiao-guang
XG
Chen
D017185:Angiostrongylus cantonensis / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D017206:Strongylida Infections / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth