Expression of the cell cycle regulators p14(ARF) and p16(INK4a) in chronic myeloid leukemia.
Expression of p14(ARF) and p16(INK4a) tumor suppressor genes was investigated in 109 patients with chronic myeloid leukemia (CML). The p14(ARF) and p16(INK4a) mRNA levels were significantly low in patients in chronic phase (CP) at presentation and high in patients treated with interferon-alpha (IFN-alpha), especially in non-responders. A moderate overexpression of p14(ARF) with a normal expression of p16(INK4a) was observed in imatinib-resistant patients. Although protein expression did not consistently match mRNA levels, a role for the two cell cycle regulators in the IFN-alpha signaling pathway is suggested as well as a relation with the resistance to IFN-alpha or imatinib therapy.
Laboratoire d'Hématologie, CHU de Poitiers, France. m.cividin@chu-poitiers.fr
Marie
M
Cividin
Olivier
O
Ayrault
Nathalie
N
Sorel
Paule
P
Séité
Françoise
F
Brizard
Odile
O
Blanchet
François-Xavier
FX
Mahon
François
F
Guilhot
Christian
C
Larsen
Jean-Claude
JC
Chomel
André
A
Brizard
D002453:Cell Cycle / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012016:Reference Values; D012189:Retrospective Studies; D015398:Signal Transduction; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics*
D019941:Cyclin-Dependent Kinase Inhibitor p16; D012333:RNA, Messenger; D025803:Tumor Suppressor Protein p14ARF