Matrix-assisted laser desorption/ionization mass spectrometric analysis of uncomplexed highly sulfated oligosaccharides using ionic liquid matrices.
Direct UV matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis of uncomplexed, underivatized, highly sulfated oligosaccharides has been carried out using ionic liquids as matrices. Under conventionally used MALDI time-of-flight experimental conditions, uncomplexed polysulfated oligosaccharides do not produce any signal. We report that 1-methylimidazolium alpha-cyano-4-hydroxycinnamate and butylammonium 2,5-dihydroxybenzoate ionic liquid matrices allow the detection of picomole amounts of the sodium salts of a disaccharide, sucrose octasulfate, and an octasulfated pentasaccharide, Arixtra. The experimental results indicate that both analytes undergo some degree of thermal fragmentation with a mass loss corresponding to cleavage of O-SO3Na bonds in the matrix upon laser irradiation, reflecting lability of sulfo groups.
Laremore
Tatiana N
TN
Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 110 8th Street, Troy, New York 12180, USA.
Murugesan
Saravanababu
S
Park
Tae-Joon
TJ
Avci
Fikri Y
FY
Zagorevski
Dmitri V
DV
Linhardt
Robert J
RJ
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002934:Cinnamates / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013431:Sulfates / Q000737:chemistry*
C518937:1-methylimidazolium alpha-cyano-4-hydroxycinnamate; D002934:Cinnamates; D007093:Imidazoles; D052578:Ionic Liquids; D009844:Oligosaccharides; D013431:Sulfates