Gene trapping techniques and current progress.
Gene trapping technique is a powerful method for gene cloning and analysis. A lot of genes with unknown functions have been discovered and characterized with the help of randomly inserted mutation libraries developed by gene trapping techniques. In this article, the origin and background of gene trapping technology are briefly introduced, and then the principles of typical gene trap vectors, current progress and new efficient gene trapping techniques are discussed in detail. Disadvantage and prospects of various gene trapping technologies are also discussed.
Li
Yuan-Yuan
YY
Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China. liyy04@mails.gucas.ac.cn
Zhang
Jing-Pu
JP
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004251:DNA Transposable Elements / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016254:Mutagenesis, Insertional / Q000379:methods*; D016297:Mutagenesis, Site-Directed / Q000379:methods
D004251:DNA Transposable Elements