Molecular identification and expression of differentially regulated genes of the European flounder, Platichthys flesus, submitted to pesticide exposure.
Effects of pesticide exposure on the European flounder, Platichthys flesus, were investigated using a suppression subtractive hybridization method (SSH) to identify up- and down-regulated genes after a 30-day exposure to herbicides (a cocktail of atrazine, diuron, and isoproturon, and a single herbicide, glyphosate). A total of 256 expressed gene sequences were identified as having the potential for being differentially expressed, of which 116 could be identified by homology with databased sequences. The metabolic functions with which they are associated include energy production, general metabolism, signaling, transport, immune system, and structure. Expression of 14 of these genes was analyzed in liver, muscle, and gills by reverse transcriptase-polymerase chain reaction (RT-PCR) under experimental conditions (0, 15, and 30 days of exposure) and under field conditions (sampling in two estuaries displaying different levels of pesticide contamination). This study provides a first basis for studying the response of fish to pesticide exposure and allows the characterization of new potential genetic markers of pesticide contamination in the field.
Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR-CNRS 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, 29280, Plouzané, France.
J
J
Marchand
A
A
Tanguy
G
G
Charrier
L
L
Quiniou
E
E
Plee-Gauthier
J
J
Laroche
D000818:Animals; D001280:Atrazine / Q000494:pharmacology; D004237:Diuron / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D005060:Europe; D005260:Female; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism*; D005432:Flounder / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D005880:Gills / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D008297:Male; D010671:Phenylurea Compounds / Q000494:pharmacology; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000494:pharmacology; D000097797:Glyphosate
D001280:Atrazine; D004237:Diuron; D029941:Fish Proteins; D005998:Glycine; D006540:Herbicides; D010671:Phenylurea Compounds; D014874:Water Pollutants, Chemical; D000097797:Glyphosate; C028904:isoproturon