Picoliter wells from selective growth of HEK293 cells on chemically modified PDMS surfaces.
In this study, a method for the rapid generation of a variety of bifunctional surfaces that can serve to quickly determine the selective adhesion of HEK293 cells towards different chemical functionalities has been established. Using the information about selective adhesion of HEK293 cells to bifunctional surfaces, we demonstrate the ability to construct stable, high density, and multi-welled surfaces where the mammalian cells form the walls of picoliter volume wells.
Industrial Materials Institute, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC, Canada.
Gerardo A
GA
Diaz-Quijada
Shirin
S
Farrahi
Jennifer
J
Clarke
Angela M
AM
Tonary
John Paul
JP
Pezacki
D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000502:physiology*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009757:Nylons / Q000737:chemistry*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer