[Effect of S-bioallethrin on human lymphocyte].
OBJECTIVE
To study the effect of the S-bioallethrin on human lymphocytes by microarray technique.


METHODS
The changes of normal human lymphocytes treated with S-bioallethrin were examined with light microscope, flow cytometry, electron microscope, DNA ladder and microarray techniques.


RESULTS
Morphological study showed that the lymphocytes underwent apoptosis after S-bioallethrin exposure, which as further confirmed by the expression changes of 346 genes.


CONCLUSIONS
S-bioallethrin can induce apoptosis of normal human lymphocytes and changes in their gene expression profiles.
Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China. sunshine@fimmu.com
Yue
Y
Liu
Li-Yan
LY
Liang
Wen-Li
WL
Ma
Wen-Ling
WL
Zheng
D000487:Allethrins / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D007306:Insecticides / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000487:Allethrins; D007306:Insecticides; C018465:bioallethrin