Synthesis and characterization of poly(L-lactic acid-co-ethylene oxide-co-aspartic acid) and its interaction with cells.
Multiblock terpolymer of poly(L-lactic acid)/poly(ethylene oxide)/poly(L-aspartic acid), (PLLA/PEO/PAsp) was synthesized by ring opening polymerization of beta -benzyl L-aspartate N-carboxyanhydride, Asp(OBzl)-NCA with alpha-omega -hydroxy terminated triblock PLLA/PEO/PLLA copolymer. The resulting multiblock terpolymer was characterized by several techniques including Fourier transform infrared spectroscopy and differential scanning calorimetry.(1)H nuclear magnetic resonance spectra indicated the molar ratio of PLLA/PEO/PAsp (OBzl) to be 86/10/4. Thermal gravimetric analysis and environmental scanning electron microscopy data showed that PLLA/PEO/PAsp had crystalline and brittle structure. In order to improve its mechanical and physical properties, the terpolymer was blended with high molecular weight poly(L-lactic-co-glycolic acid) copolymer, PLGA(85/15) (M(w): 95000 gmol(-1)) in 25/75 and 50/50 mole ratios. The hydrolytical degradation properties of these polymers were studied. Degradation experiments were performed during a 48-day period in pH:7.4 phosphate-buffered saline (PBS) at 37 degrees C. The observed molecular weight losses were 91% and 67% for the 25/75 and 50/50 mixtures, respectively. In vitro attachment and growth of L929 mouse fibroblasts on these biopolymers were also investigated. Cell growth experiments indicated that the copolymer blend allowed the attachment and growth of cells.
TUBITAK, Research Institute for Genetic Engineering and Biotechnology, Gebze-Kocaeli, Turkey.
Okşan
O
Karal-Yilmaz
Nilhan
N
Kayaman-Apohan
Zülal
Z
Misirli
Kemal
K
Baysal
Bahattin M
BM
Baysal
D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry*; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D001673:Biodegradation, Environmental / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008958:Models, Molecular; D011100:Polyglycolic Acid / Q000138:chemical synthesis* / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000138:chemical synthesis* / Q000494:pharmacology; D013499:Surface Properties; D013696:Temperature; D023822:Tissue Engineering
D001672:Biocompatible Materials; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D001224:Aspartic Acid; D019344:Lactic Acid