Mapping and comprehensive analysis of the extracellular and cell surface proteome of the human pathogen Corynebacterium diphtheriae.
Secreted proteins of the human pathogen Corynebacterium diphtheriae might be involved in important pathogen-host cell interactions. Here, we present the first systematic reference map of the extracellular and cell surface proteome fractions of the type strain C. diphtheriae C7s(-)tox-. The analysis window of 2-DE covered the pI range from 3 to 10 along with a MW range from 8 to 150 kDa. Computational analysis of the 2-D gels detected almost 150 protein spots in the extracellular proteome fraction and about 80 protein spots of the cell surface proteome. MALDI-TOF-MS and PMF with trypsin unambiguously identified 107 extracellular protein spots and 53 protein spots of the cell surface, representing in total 85 different proteins of C. diphtheriae C7s(-)tox-. Several of the identified proteins are encoded by pathogenicity islands and might represent virulence factors of C. diphtheriae. Additionally, four solute-binding proteins (HmuT, Irp6A, CiuA, and FrgD) of different iron ABC transporters were identified, with the hitherto uncharacterized FrgD protein being the most abundant one of the cell surface proteome of C. diphtheriae C7s(-)tox-.
Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Universitätsstrasse, Bielefeld, Germany.
Nicole
N
Hansmeier
Tzu-Chiao
TC
Chao
Jörn
J
Kalinowski
Alfred
A
Pühler
Andreas
A
Tauch
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods; D003353:Corynebacterium diphtheriae / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D008074:Lipoproteins / Q000737:chemistry; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D040901:Proteomics / Q000379:methods*; D012984:Software
D001426:Bacterial Proteins; C408133:HmuT protein, Corynebacterium diphtheriae; D008074:Lipoproteins; D008565:Membrane Proteins; D021382:Protein Sorting Signals