Reversal of diabetes in non-obese diabetic mice without spleen cell-derived beta cell regeneration.
Autoimmune destruction of beta cells is the predominant cause of type 1 diabetes mellitus (T1DM) in humans and is modeled in non-obese diabetic (NOD) mice. Many therapeutic interventions prevent the development of T1DM in NOD mice, but few can induce its reversal once established. Intervention with Freund's complete adjuvant, semi-allogeneic splenocytes, and temporary islet transplantation has been reported to cure NOD mice of established T1DM. Using the same approach, we report here that this treatment cured 32% of NOD mice of established diabetes (>340 milligrams per deciliter blood glucose), although beta cells in these mice were not derived from donor splenocytes.
Chong
Anita S
AS
Section of Transplantation, Department of Surgery, University of Chicago, Chicago, IL 60637, USA. achong@uchicago.edu
Shen
Jikun
J
Tao
Jing
J
Yin
Dengping
D
Kuznetsov
Andrey
A
Hara
Manami
M
Philipson
Louis H
LH
D000818:Animals; D015551:Autoimmunity; D001786:Blood Glucose / Q000032:analysis; D002454:Cell Differentiation; D017690:Cell Transplantation; D003131:Combined Modality Therapy; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D005260:Female; D005620:Freund's Adjuvant / Q000627:therapeutic use*; D049452:Green Fluorescent Proteins / Q000032:analysis; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000502:physiology; D016381:Islets of Langerhans Transplantation; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008822:Mice, Transgenic; D012038:Regeneration; D013154:Spleen / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
D001786:Blood Glucose; D049452:Green Fluorescent Proteins; D005620:Freund's Adjuvant