Rapid molecular dissection of viral and bacterial immunomes.
The development of preventive or therapeutic recombinant vaccines and the generation of serodiagnostic assays for infectious diseases depend essentially on the availability of molecularly defined antigens. A major bottleneck for the identification of suitable target antigens for many pathogens is the isolation of sufficient amounts of material for subsequent genomic or proteomic screening. Applying a highly efficient expression cloning strategy to the human pathogens vaccinia virus (VV) and Chlamydia pneumoniae (CP), we demonstrate that sub-nanogram amounts of isolated nucleic acids can be utilized to determine comprehensive sets of immunodominant antigens. Remarkably, the approach not only confirmed the immunogenicity of previously reported antigens but also disclosed novel vaccine candidates conserved in orthopoxviruses, including antigenic envelope proteins and immunodominant CTL epitopes. Moreover, as illustrated for CP infection, we show that a panel of novel antigens can be readily selected from the initially discovered pool to build up pathogen-specific seroassays. The established approach is rapid, making it an attractive procedure for the comprehensive dissection of immunomes of known human pathogens and newly emerging infectious agents.
III. Department of Internal Medicine, Johannes Gutenberg University Mainz, Mainz, Germany. Sahin@MAIL.UNI-MAINZ.DE
Ugur
U
Sahin
Ozlem
O
Türeci
Claudine
C
Graf
Ralf G
RG
Meyer
Volker
V
Lennerz
Cedrik M
CM
Britten
Claudia
C
Dumrese
Elke
E
Scandella
Thomas
T
Wölfel
Burkhard
B
Ludewig
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000942:Antigens, Bacterial; D000956:Antigens, Viral; D004269:DNA, Bacterial; D004279:DNA, Viral; D018984:Epitopes, T-Lymphocyte; D016056:Immunodominant Epitopes