Biotechnological traps for the reduction of inflammation due to cardiopulmonary bypass operations.
Cardiopulmonary bypass induces a systemic inflammatory response (SIR), characterized by the activation of cellular and humoral elements, with concomitant release of neutrophil elastase and matrix-metallo proteinases. In the present study, the protease release during extracorporeal circulation in 28 patients undergoing cardiac surgical operations was monitored using casein zymography. A peak in protease activity was found in all patients at the end of cardiopulmonary bypass. Plasma samples of patients were allowed to interact with different traps obtained by immobilizing different protease inhibitors on specific carriers. alpha1-Antitrypsin, Bovine Pancreatic Trypsin Inhibitor, Elastatinal or Leupeptin were used as inhibitors and were covalently immobilized by diazotization or by condensation. A reduction in the proteolytic activity of the plasma samples was observed after interaction with the different traps. The most efficient traps, i.e. the ones displaying greatest power to inhibit protease activity, were those obtained by immobilizing Bovine Pancreatic Trypsin Inhibitor and Leupeptin. The biocompatibility of traps was also tested. Results show that protease activity in blood can be decreased by our protease traps.
Grano
Valentina
V
Department of Experimental Medicine, Biotechnology and Molecular Biology Section, Second University of Naples, Via S. M. di Costantinopoli 16, Naples 80138, Italy.
Salamino
Franca
F
Melloni
Edon
E
Minafra
Roberto
R
Regola
Eliana
E
Diano
Nadia
N
Nicolucci
Carla
C
Attanasio
Angelina
A
Nappi
Gianantonio
G
Cotrufo
Maurizio
M
Maresca
Lucio
L
De Santo
Natale Gaspare
NG
Mita
Damiano Gustavo
DG
D000818:Animals; D001709:Biotechnology; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000209:etiology / Q000628:therapy; D008567:Membranes, Artificial; D008875:Middle Aged; D015394:Molecular Structure; D010447:Peptide Hydrolases / Q000097:blood*
D004791:Enzyme Inhibitors; D008567:Membranes, Artificial; D010447:Peptide Hydrolases