Examination of drug resistance activity of human TAP-like (ABCB9) expressed in yeast.
A half-type ABC transporter, human TAP-like (hTAPL) tagged with histidine cluster, was expressed in budding yeast protease-deficient strain BJ5457, and the effect of expression for resistance to peptide compounds including antibiotics and proteinase inhibitor was examined. Among these compounds, the yeast expressing hTAPL exhibits high sensitivity to valinomycin, a monovalent cation ionophore. A mutation in Walker A motif, which lost ATP-binding activity of hTAPL, eliminated the enhanced sensitivity to valinomycin. These findings suggest that the transport activity of hTAPL is important for conferring high valinomycin-sensitive phenotype to yeast.
Ohashi-Kobayashi
Ayako
A
Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
Ohashi
Kazuaki
K
Du
Wei-Bin
WB
Omote
Hiroshi
H
Nakamoto
Robert
R
Al-Shawi
Marwan
M
Maeda
Masatomo
M
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000900:Anti-Bacterial Agents; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D006801:Humans; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014634:Valinomycin / Q000008:administration & dosage*
C412573:ABCB9 protein, human; D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D014634:Valinomycin