Evaluation of p53 codon 72 polymorphism in adenocarcinomas of the colon and rectum in La Plata, Argentina.
OBJECTIVE
To evaluate the potential association between p53 codon 72 polymorphism and sporadic colorectal adenocarcinoma development,and human papillomavirus (HPV) infection.


METHODS
One-hundred and nine controls and 53 patients with colon cancer from the city of La Plata, Argentina were analyzed. p53 codon 72 genotypes and HPV infection were identified using allele-specific polymerase chain reaction and nested polymerase chain reaction, respectively.


RESULTS
The differences in the distribution of p53 codon 72 polymorphism between the cases and controls were statistically significant. The arginine allele had a prevalence of 0.65 in controls and 0.77 in cases. The corresponding odds ratio for the homozygous arginine genotype was 2.08 (95% CI, 1.06-4.05; P<0.05). Lack of association was found between p53 polymorphism and HPV infection in the set of adenocarcinomas.


CONCLUSIONS
The findings of the present study indicate that p53 codon 72 arginine homozygous genotype may represent a genetic predisposing factor for colon cancer development. However,further studies are needed in order to elucidate the role of p53 codon 72 polymorphism in colorectal cancer.
Centro de Investigaciones en Genética Básica y Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Calle 60 y 118 S/N, B 1900 AVW, La Plata, Argentina. prezluis@yahoo.com.ar
Luis Orlando
LO
Pérez
Martin Carlos
MC
Abba
Fernando Noel
FN
Dulout
Carlos Daniel
CD
Golijow
D000230:Adenocarcinoma / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001118:Argentina; D016022:Case-Control Studies; D015984:Causality; D003062:Codon / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics*; D016158:Genes, p53; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D003062:Codon