Phylogenetic analysis of Glomeromycota by partial LSU rDNA sequences.
We analyzed the large subunit ribosomal RNA (rRNA) gene [LSU ribosomal DNA (rDNA)] as a phylogenetic marker for arbuscular mycorrhizal (AM) fungal taxonomy. Partial LSU rDNA sequences were obtained from ten AM fungal isolates, comprising seven species, with two new primers designed for Glomeromycota LSU rDNA. The sequences, together with 58 sequences available from the databases, represented 31 AM fungal species. Neighbor joining and parsimony analyses were performed with the aim of evaluating the potential of the LSU rDNA for phylogenetic resolution. The resulting trees indicated that Archaeosporaceae are a basal group in Glomeromycota, Acaulosporaceae and Gigasporaceae belong to the same clade, while Glomeraceae are polyphyletic. The results support data obtained with the small subunit (SSU) rRNA gene, demonstrating that the LSU rRNA gene is a useful molecular marker for clarifying taxonomic and phylogenetic relationships in Glomeromycota.
Departamento de Micologia, CCB, Universidade Federal de Pernambuco, Av. Prof. Nelson Chaves, s/n., 50670-420, Recife, PE, Brazil.
Gladstone Alves
GA
da Silva
Istituto per la Protezione delle Piante (Sezione di Torino) del CNR and Dipartimento di Biologia Vegetale dell' Università-Viale P.A., Mattioli 25, 10125, Torino, Italy.
Erica
E
Lumini
Departamento de Micologia, CCB, Universidade Federal de Pernambuco, Av. Prof. Nelson Chaves, s/n., 50670-420, Recife, PE, Brazil.
Leonor Costa
LC
Maia
Istituto per la Protezione delle Piante (Sezione di Torino) del CNR and Dipartimento di Biologia Vegetale dell' Università-Viale P.A., Mattioli 25, 10125, Torino, Italy.
Paola
P
Bonfante
Istituto per la Protezione delle Piante (Sezione di Torino) del CNR and Dipartimento di Biologia Vegetale dell' Università-Viale P.A., Mattioli 25, 10125, Torino, Italy.
Valeria
V
Bianciotto
D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D038821:Mycorrhizae / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D013045:Species Specificity
D017931:DNA Primers; D004271:DNA, Fungal; D004275:DNA, Ribosomal