Encircled: large-scale purification of replication origins from Mammalian chromosomes.
A novel cloning strategy for sequences comprising mammalian replication origins, described by Mesner et al. (2006) in a recent issue of Molecular Cell, utilizes an origin trapping assay in which replication bubbles are selectively retained in agarose due to their circular nature.
Bielinsky
Anja-Katrin
AK
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, 55455, USA.
Raveendranathan
Miruthubashini
M
D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D005057:Eukaryotic Cells; D015723:Gene Library; D018741:Replication Origin / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D013762:Tetrahydrofolate Dehydrogenase