A novel heterobifunctional linker for facile access to bioconjugates.
A convenient synthesis of a novel heterobifunctional linker molecule is described. The linker contains a thiol-reactive nitropyridyl disulfide group (Npys) and an aldehyde-reactive aminooxy group with a propensity to form disulfide and oxime linkages. The utility of the linker molecule to cross-link different biomolecules has been demonstrated by employing it in the efficient preparation of a peptide-oligonucleotide conjugate. The linker reported herein could be a useful tool for cross-coupling of different but appropriately functionalised biomolecules.
Singh
Yashveer
Y
LEDSS, UMR CNRS 5616, ICMG FR 2607, Université Joseph Fourier, 301 rue de la Chimie, BP 53, F 38041, Grenoble Cedex 9, France.
Spinelli
Nicolas
N
Defrancq
Eric
E
Dumy
Pascal
P
D000447:Aldehydes; D001685:Biological Factors / Q000138:chemical synthesis* / Q000737:chemistry*; D002942:Circular Dichroism; D003432:Cross-Linking Reagents / Q000138:chemical synthesis* / Q000737:chemistry*; D003545:Cysteine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D004220:Disulfides; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D009842:Oligopeptides; D013053:Spectrophotometry; D013816:Thermodynamics
D000447:Aldehydes; D001685:Biological Factors; D003432:Cross-Linking Reagents; D004220:Disulfides; D007202:Indicators and Reagents; D009841:Oligonucleotides; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D003545:Cysteine