In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system.
Position-specific incorporation of non-natural amino acids into proteins is a useful technique in protein engineering. In this study, we established a novel selection system to obtain tRNAs that show high decoding activity, from a tRNA library in a cell-free translation system to improve the efficiency of incorporation of non-natural amino acids into proteins. In this system, a puromycin-tRNA conjugate, in which the 3'-terminal A unit was replaced by puromycin, was used. The puromycin-tRNA conjugate was fused to a C-terminus of streptavidin through the puromycin moiety in the ribosome. The streptavidin-puromycin-tRNA fusion molecule was collected and brought to the next round after amplification of the tRNA sequence. We applied this system to select efficient frameshift suppressor tRNAs from a tRNA library with a randomly mutated anticodon loop derived from yeast tRNA CCCG Phe. After three rounds of the selection, we obtained novel frameshift suppressor tRNAs which had high decoding activity and good orthogonality against endogenous aminoacyl-tRNA synthetases. These results demonstrate that the in vitro selection system developed here is useful to obtain highly active tRNAs for the incorporation of non-natural amino acid from a tRNA library.
Taira
Hikaru
H
Department of Bioscience and Bioengineering, Okayama University, Tsushimanaka, Okayama 700-8530, Japan.
Hohsaka
Takahiro
T
Sisido
Masahiko
M
D000596:Amino Acids / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D000926:Anticodon / Q000737:chemistry; D001483:Base Sequence; D002474:Cell-Free System; D003062:Codon / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics*; D018965:Frameshifting, Ribosomal; D015723:Gene Library; D008969:Molecular Sequence Data; D009154:Mutation; D014176:Protein Biosynthesis; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry; D011691:Puromycin / Q000737:chemistry; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019809:Streptavidin / Q000737:chemistry; D015003:Yeasts / Q000235:genetics
D000596:Amino Acids; D000926:Anticodon; D003062:Codon; D011506:Proteins; D012360:RNA, Transfer, Phe; D011691:Puromycin; D019809:Streptavidin; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases