Synthesizing cellular networks from evolved ribosome-mRNA pairs.
We describe cellular engineering for the creation of multiple new cellular modules each composed of an orthogonal ribosome and orthogonal mRNA. These modules operate independently of the endogenous ribosome and mRNA. We discuss some of the applications of orthogonal pairs and highlight the expression of Boolean logic in gene regulation using multiple orthogonal pairs.
Rackham
O
O
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Chin
J W
JW
D005075:Biological Evolution; D005786:Gene Expression Regulation; D011485:Protein Binding; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism*
D012333:RNA, Messenger