Immunization with heat shock protein 105-pulsed dendritic cells leads to tumor rejection in mice.
Recently, we reported that heat shock protein 105 (HSP105) DNA vaccination induced anti-tumor immunity. In this study, we set up a preclinical study to investigate the usefulness of dendritic cells (DCs) pulsed with mouse HSP105 as a whole protein for cancer immunotherapy in vivo. The recombinant HSP105 did not induce DC maturation, and the mice vaccinated with HSP105-pulsed BM-DCs were markedly prevented from the growth of subcutaneous tumors, accompanied with a massive infiltration of both CD4+ T cells and CD8+ T cells into the tumors. In depletion experiments, we proved that both CD4+ T cells and CD8+ T cells play a crucial role in anti-tumor immunity. Both CD4+ T cells and CD8+ T cells specific to HSP105 were induced by stimulation with HSP105-pulsed DCs. As a result, vaccination of mice with BM-DCs pulsed with HSP105 itself could elicit a stronger tumor rejection in comparison to DNA vaccination.
Department of Immunogenetics, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
Kazunori
K
Yokomine
Tetsuya
T
Nakatsura
Motozumi
M
Minohara
Jun-ichi
J
Kira
Tatsuko
T
Kubo
Yutaka
Y
Sasaki
Yasuharu
Y
Nishimura
D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology*; D050957:HSP110 Heat-Shock Proteins / Q000276:immunology* / Q000494:pharmacology*; D007114:Immunization; D008212:Lymphocyte Depletion; D051379:Mice; D008815:Mice, Inbred Strains; D009369:Neoplasms / Q000276:immunology / Q000628:therapy*; D014611:Vaccination
D019496:Cancer Vaccines; D050957:HSP110 Heat-Shock Proteins; C490783:Hsp105 protein, mouse