[Study on the relationship between DNA damage and polymorphisms of metabolizing enzymes of vinyl chloride monomer-exposed workers].
OBJECTIVE
To explore the relationship between DNA damage induced by vinyl chloride monomer (VCM) and polymorphisms of metabolizing enzymes of VCM.


METHODS
Comet assay was employed to detect DNA damage, the workers were divided into two groups based on the status of DNA damage. Case-control design was used to investigate the relationship between the genetic polymorphisms of metabolizing enzymes and DNA damage. Genotypes of CYP2E1 c1/c2 and mEH4 His139Arg were identified by the PCR-RFLP, null genotypes of GST T1 and GST M1 were detected by PCR.


RESULTS
The genotypes of CYP2E1 c1c2 and c2c2 were significantly associated with DNA damages (P < 0.01). A prominent risk increasing of DNA damage was observed for those individuals having a high or low VCM exposure and possessing the CYP2E1 c1c2 and c2c2 genotypes (OR 4.92, 95% CI 1.35-13.85 and OR 2.57, 95% CI 1.01-6.59).


CONCLUSIONS
Cumulative exposure dose and polymorphism of metabolizing enzymes may modulate the DNA damage of VCM-exposed workers. possessing the CYP2E1 c1c2 and c2c2 genotypes (OR 4.92, 95% CI 1.35-13.85 and OR 2.57, 95% CI 1.01-6.59).


CONCLUSIONS
Cumulative exposure dose and polymorphism of metabolizing enzymes may modulate the DNA damage of VCM-exposed workers.
School of Public Health, Fudan University, Shanghai 200032, China.
Shou-min
SM
Zhu
Wei
W
Cheng
Feng-ren
FR
Du
Ai-hong
AH
Wang
D000293:Adolescent; D000328:Adult; D002617:Chemical Industry; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D004249:DNA Damage; D004798:Enzymes / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D011110:Polymorphism, Genetic / Q000187:drug effects*; D014752:Vinyl Chloride / Q000009:adverse effects*; D055815:Young Adult
D004798:Enzymes; D019392:Cytochrome P-450 CYP2E1; D014752:Vinyl Chloride