A tick B-cell inhibitory protein from salivary glands of the hard tick, Hyalomma asiaticum asiaticum.
Some studies done to date suggest that B-cell inhibitory factor occurred in tick saliva. In this study, a novel protein having B-cell inhibitory activity was purified and characterized from the salivary glands of the hard tick, Hyalomma asiaticum asiaticum. This protein was named B-cell inhibitory factor (BIF). The cDNA encoding BIF was cloned by cDNA library screening. The predicted protein from the cDNA sequence is composed of 138 amino acids including the mature BIF. No similarity was found by Blast search. The lipopolysaccharide-induced B-cell proliferation was inhibited by BIF. This is the first report of the identification and characterization of B-cell inhibitory protein from tick. The current study facilitates the study of identifying the interaction among tick, Borrelia burgdorferi, the causative agent of Lyme disease, and host.
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Da
D
Yu
Jiangguo
J
Liang
Haining
H
Yu
Haifeng
H
Wu
Chunhua
C
Xu
Jingze
J
Liu
Ren
R
Lai
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D026863:Ixodidae / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D012469:Salivary Glands / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000737:chemistry* / Q000494:pharmacology*
C510193:B-cell inhibitory factor, Hyalomma asiaticum; D012471:Salivary Proteins and Peptides