Immunological reversal of autoimmune diabetes without hematopoietic replacement of beta cells.
Type 1 diabetes mellitus results from the autoimmune destruction of the beta cells of the pancreatic islets of Langerhans and is recapitulated in the nonobese diabetic strain of mice. In an attempt to rescue islet loss, diabetic mice were made normoglycemic by islet transplantation and immunization with Freund's complete adjuvant along with multiple injections of allogeneic male splenocytes. This treatment allowed for survival of transplanted islets and recovery of endogenous beta cell function in a proportion of mice, but with no evidence for allogeneic splenocyte-derived differentiation of new islet beta cells. Control of the autoimmune disease at a crucial time in diabetogenesis can result in recovery of beta cell function.
Suri
Anish
A
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA. anish@pathology.wustl.edu
Calderon
Boris
B
Esparza
Thomas J
TJ
Frederick
Katherine
K
Bittner
Patrice
P
Unanue
Emil R
ER
D000818:Animals; D015551:Autoimmunity; D001786:Blood Glucose / Q000032:analysis; D002452:Cell Count; D017690:Cell Transplantation; D003131:Combined Modality Therapy; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D005260:Female; D005620:Freund's Adjuvant / Q000627:therapeutic use*; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000502:physiology*; D007515:Islets of Langerhans / Q000473:pathology; D016381:Islets of Langerhans Transplantation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D013154:Spleen / Q000166:cytology* / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D001786:Blood Glucose; D005620:Freund's Adjuvant