Amplification of 1-deoxy-D-xyluose 5-phosphate (DXP) synthase level increases coenzyme Q10 production in recombinant Escherichia coli.
For the enhancement of coenzyme Q(10) (CoQ(10)) production, 1-deoxy-D-xylulose 5-phosphate (DXP) synthase of Pseudomonas aeruginosa was constitutively coexpressed in a recombinant Escherichia coli strain, which harbors the ddsA gene from Gluconobacter suboxydans encoding decaprenyl diphosphate synthase. It was found that the expression of the ddsA gene caused depletion of the isopentenyl diphosphate (IPP) pool in E. coli. Amplification of DXP synthase level by installing P. aeruginosa DXP synthase restored the diminished IPP pool and concomitantly resulted in approximately a twofold increase in relative content and productivity of CoQ(10). Maximum CoQ(10) concentration of 46.1 mg l(-1) was achieved from glucose-limited fed-batch cultivation of the recombinant E. coli strain simultaneously harboring the ddsA and dxs genes.
Department of Agricultural Biotechnology and Research Center for Agricultural Biomaterials, Seoul National University, Seoul, 151-921, South Korea.
Soo-Jung
SJ
Kim
Myoung-Dong
MD
Kim
Jin-Ho
JH
Choi
Sang-Yong
SY
Kim
Yeon-Woo
YW
Ryu
Jin-Ho
JH
Seo
D003067:Coenzymes; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D020600:Gluconobacter oxydans / Q000201:enzymology / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics; D014166:Transferases / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism
D003067:Coenzymes; D014451:Ubiquinone; D014166:Transferases; C109461:deoxyxylulose-5-phosphate synthase; C024989:coenzyme Q10