[Analysis and application of excitation fluorescence intensity of blood].
The analysis technique of fluorescence is adopted to study the intensity of excitation fluorescence of blood in the present paper. The theoretical analysis and differences of normal and abnormal blood (hyperglycemia, hyperlipemia) are presented. The theoretical analysis was proved by experiment results. It was discovered that blood sugar consistency has an effect on blood fluorescence. In other words, with the same excitation wavelength and with blood sugar consistency increasing, the fluorescence intensity increases gradually. It is obvious that blood sugar is also a kind of fluorescein, and its consistency has an effect on fluorescence intensity, which is identical with the theoretical analysis, indicating that the experiment is successful, and it is possible to distinguish blood sugar consistency by comparing the fluorescence intensity in blood. It was also discovered that the higher the cholesterin content, the more intense the fluorescence. When excitation wavelength is especially 435 nm, the phenomenon is very obvious. The study paves a new way for the blood quick check and diagnosis of diseases.
College of Science, Nanjing Uiversity of Aeronautics and Astronautics, Nanjing 210016, China.
Zhi-Min
ZM
Zhao
Lin-Feng
LF
Guo
Xiao-Lei
XL
Yu
Jun-Yan
JY
Ma
Le-Xin
LX
Wang
Yu-Jun
YJ
Xin
D001774:Blood Chemical Analysis / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D006801:Humans; D013050:Spectrometry, Fluorescence / Q000379:methods*
D001786:Blood Glucose