GEM System: automatic prototyping of cell-wide metabolic pathway models from genomes.
BACKGROUND
Successful realization of a "systems biology" approach to analyzing cells is a grand challenge for our understanding of life. However, current modeling approaches to cell simulation are labor-intensive, manual affairs, and therefore constitute a major bottleneck in the evolution of computational cell biology.


RESULTS
We developed the Genome-based Modeling (GEM) System for the purpose of automatically prototyping simulation models of cell-wide metabolic pathways from genome sequences and other public biological information. Models generated by the GEM System include an entire Escherichia coli metabolism model comprising 968 reactions of 1195 metabolites, achieving 100% coverage when compared with the KEGG database, 92.38% with the EcoCyc database, and 95.06% with iJR904 genome-scale model.


CONCLUSIONS
The GEM System prototypes qualitative models to reduce the labor-intensive tasks required for systems biology research. Models of over 90 bacterial genomes are available at our web site.
Arakawa
Kazuharu
K
Institute for Advanced Biosciences, Keio University, Fujisawa 252-8520, Japan. gaou@sfc.keio.ac.jp
Yamada
Yohei
Y
Shinoda
Kosaku
K
Nakayama
Yoichi
Y
Tomita
Masaru
M
D002874:Chromosome Mapping / Q000379:methods*; D003198:Computer Simulation; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D016680:Genome, Bacterial / Q000235:genetics; D008954:Models, Biological; D010865:Pilot Projects; D025941:Protein Interaction Mapping / Q000379:methods; D015398:Signal Transduction / Q000502:physiology*; D012984:Software
D029968:Escherichia coli Proteins