Estrogen alters c-Fos response to immobilization stress in the brain of ovariectomized rats.
Estrogen receptors are widely expressed in the brain, where estrogen modulates central nervous function. In this study, we investigated the effect of estrogen on the emotional stress response in the brain by comparing the CNS patterns of c-Fos expression in response to immobilization stress (IMO) in ovariectomized rats with placebo treatment (OVX + Pla) vs. ovariectomized rats supplemented with 17beta-estradiol (OVX + E2). Increased c-Fos immunoreactive neurons in response to IMO were observed in cerebral cortex, septum, thalamus, hypothalamus, midbrain, pons and medulla oblongata in accordance with previous findings. When OVX + E2/Stress were compared with OVX + Pla/Stress, the numbers of c-Fos immunoreactive cells were significantly lower in the lateral septum, paraventricular hypothalamic nucleus, dorsomedial hypothalamic nucleus, medial amygdaloid nucleus, lateral periaqueductal gray, laterodorsal tegmental nucleus and locus coeruleus, while they were significantly higher in paraventricular thalamic nucleus and nucleus of the solitary tract. These data suggest that neuronal activities in these areas are influenced bidirectionally by systemic estrogen level.
Ueyama
Takashi
T
Department of Anatomy and Cell Biology, Wakayama Medical University, Kimiidera 811-1, Wakayama 641-8509, Japan. tueyama@wakayama-med.ac.jp
Tanioku
Tadashi
T
Nuta
Junya
J
Kujira
Kazuto
K
Ito
Takao
T
Nakai
Saori
S
Tsuruo
Yoshihiro
Y
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004958:Estradiol / Q000008:administration & dosage* / Q000097:blood; D005260:Female; D007839:Functional Laterality; D005786:Gene Expression Regulation / Q000187:drug effects*; D007103:Immobilization / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D010052:Ovariectomy / Q000379:methods; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000188:drug therapy*
D016760:Proto-Oncogene Proteins c-fos; D004958:Estradiol