Human plasma adenosine deaminase 2 is secreted by activated monocytes.
Adenosine deaminase (ADA) plays an important role in the immune system, and its activity is composed of two kinetically distinct isozymes, ADA1 and ADA2. ADA2 is a major component of human plasma total ADA activity and ADA2 activity is significantly elevated in patients with various diseases such as HIV infection and chronic hepatitis. However, relatively little is known about ADA2 because of difficulties in purifying this enzyme. In this study we succeeded in purifying human plasma ADA2 and demonstrate the extracellular secretion of ADA2 from human peripheral blood monocytes stimulated with phorbol 12-myristate 13-acetate and calcium ionophore.
Hokkaido Pharmaceutical University School of Pharmacy, 7-1 Katsuraoka-cho, Otaru 047-0264, Japan. sachiko@hokuyakudai.ac.jp
Sachiko
S
Iwaki-Egawa
Takaya
T
Yamamoto
Yasuhiro
Y
Watanabe
D000243:Adenosine Deaminase / Q000097:blood / Q000302:isolation & purification*; D002118:Calcium / Q000378:metabolism; D004796:Clinical Enzyme Tests; D004591:Electrophoresis, Polyacrylamide Gel; D015658:HIV Infections / Q000175:diagnosis; D006521:Hepatitis, Chronic / Q000175:diagnosis; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D007527:Isoenzymes / Q000097:blood*; D008970:Molecular Weight; D009000:Monocytes / Q000201:enzymology / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology
D007476:Ionophores; D007527:Isoenzymes; C016658:phorbolol myristate acetate; D000243:Adenosine Deaminase; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium