LRRK2 expression linked to dopamine-innervated areas.
OBJECTIVE
Leucine-rich repeat kinase 2 (LRRK2) has been linked to Parkinson's disease. Our study explores the expression of LRRK2 in human and rodent brain tissue.


METHODS
We analyzed LRRK2 gene activity at the cellular level using in situ hybridization.


RESULTS
We found a high and strikingly specific expression of LRRK2 mRNA in rodent striatum and parts of cortex and no signals in dopamine neurons. In human tissue, LRRK2 mRNA was found in the corresponding brain areas caudatus and putamen at lower levels and dopamine neurons were also devoid of LRRK2 mRNA. Expression levels in striatal tissue did not differ between Parkinson's disease patients and control subjects.


CONCLUSIONS
Unlike all other genes so far linked to Parkinson's disease, our results demonstrate that LRRK2 expression is particularly high in brain dopaminoceptive areas.
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Dagmar
D
Galter
Marie
M
Westerlund
Andrea
A
Carmine
Eva
E
Lindqvist
Olof
O
Sydow
Lars
L
Olson
D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D016022:Case-Control Studies; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D010300:Parkinson Disease / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; C495280:LRRK2 protein, human; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D017346:Protein Serine-Threonine Kinases; D004298:Dopamine