Analysis of donor-type chimerism in lineage-specific cell populations after allogeneic myeloablative and non-myeloablative stem cell transplantation.
We analyzed donor-type chimerism in CD3+, CD14.15+ and CD56+ cells from 36 patients who had undergone conventional-intensity allogeneic stem cell transplantation (CST) and 34 patients who had undergone non-myeloablative allogeneic stem cell transplantation (NST) for hematological malignancies. On day 28 after transplantation, all fractions in NST patients and CD3+ cells in CST patients who received a non-total body irradiation (TBI) regimen showed more frequent mixed chimerism (<90% donor cells) than those in patients who had received TBI. NST patients with acute graft-versus-host disease (grade II-IV) frequently showed more than 50% donor-type chimerism in CD3+ cells on day 14 (P=0.029). NST patients with <50% donor-type chimerism on day 14 and with <90% donor-type chimerism on day 28 in CD56+ cells had significantly poor 1-year overall survival (0 vs 91%, P<0.001 and 20 vs 74%, P=0.002, respectively). Both NST and CST patients with <90% donor-type chimerism in CD14.15+ cells on day 28 had significantly poor 1-year overall survival (14 vs 70%, P=0.005 and 0 vs 66%, P=0.002, respectively). Our data show that the extent of donor-type chimerism in lineage-specific cells appears to have an impact on outcome after allogeneic stem cell transplantation.
Department of Hematology and Oncology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Y
Y
Miura
J
J
Tanaka
T
T
Toubai
Y
Y
Tsutsumi
N
N
Kato
D
D
Hirate
M
M
Kaji
J
J
Sugita
A
A
Shigematsu
N
N
Iwao
S
S
Ota
N
N
Masauzi
T
T
Fukuhara
M
M
Kasai
M
M
Asaka
M
M
Imamura
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis / Q000097:blood; D005260:Female; D019337:Hematologic Neoplasms / Q000276:immunology / Q000401:mortality / Q000628:therapy*; D006801:Humans; D007938:Leukemia / Q000145:classification / Q000276:immunology / Q000401:mortality / Q000628:therapy; D008297:Male; D008875:Middle Aged; D033581:Stem Cell Transplantation; D016019:Survival Analysis; D013997:Time Factors; D014019:Tissue Donors / Q000706:statistics & numerical data*; D018183:Transplantation Chimera; D014184:Transplantation, Homologous
D015703:Antigens, CD