Highly sensitive spectrofluorimetric determination of trace amounts of lecithin using a norfloxacin-terbium probe.
BACKGROUND
Lecithin is found throughout biological systems. A new spectrofluorimetric method was developed for the determination of trace amounts of lecithin.


METHODS
Optimum conditions for the determination of lecithin were investigated.


RESULTS
Using norfloxacin (NF)-terbium ion (Tb3+) as a fluorescent probe, in a buffer solution at pH=6.80, lecithin reduced the fluorescence intensity of the NF-Tb3+ complex at lambda=545 nm; the reduced fluorescence intensity of the Tb3+ ion is proportional to the concentration of lecithin. The linear range and detection limit for the determination of lecithin were 1.08 x 10(-6)-3.02 x 10(-5) mol/l and 2.54 x 10(-7) mol/l.


CONCLUSIONS
This method is simple, practical and relatively free of interference from coexisting substances, and can be successfully applied to assess lecithin in serum samples.
Department of Chemistry, Shandong Normal University, Jinan, 250014, P R China.
Weiwei
W
Bian
Chongqiu
C
Jiang
D002412:Cations / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009643:Norfloxacin / Q000097:blood* / Q000737:chemistry; D010713:Phosphatidylcholines / Q000096:biosynthesis / Q000097:blood* / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000097:blood* / Q000737:chemistry; D013997:Time Factors
D002412:Cations; D005456:Fluorescent Dyes; D010713:Phosphatidylcholines; D013725:Terbium; D009643:Norfloxacin