Molecular cloning and characterisation of two homologues of Mannose-Binding Lectin in rainbow trout.
Mannose-binding lectin (MBL) is a C-type lectin which participates in the innate immune system as an activator of the complement system and as opsonin after binding to certain carbohydrate structures on microorganisms and pathogens. C-type lectins are all Ca(2+)-dependent molecules and they share a tightly folded carbohydrate recognition domain (CRD). In this report the isolation and characterisation of cDNA transcripts encoding two mannose-binding lectin isoforms MBL-1 and MBL-2 from rainbow trout (Oncorhynchus mykiss) is presented. The deduced amino acid sequences of trout MBL-1 and MBL-2 (185 and 186 aa, respectively) present 83% identity to each other, exhibiting the highest identity score 46, 46 and 42% with the Atlantic salmon, shishamo smelt and zebrafish counterparts, respectively. The identity to birds and mammalian MBLs ranges from 25 to 33%. The trout MBL-1 and MBL-2 contain the EPN motif of mannose-binding C-type lectins, important for mannose specificity and they are expressed exclusively in liver and spleen, respectively.
Nikolakopoulou
Konstantina
K
Department of Biology, School of Medicine, University of Patras, Rion Panepistimioupolis, 26500 Patras, Greece.
Zarkadis
Ioannis K
IK
D000199:Actins / Q000032:analysis / Q000096:biosynthesis; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling / Q000662:veterinary; D008099:Liver / Q000502:physiology; D037241:Mannose-Binding Lectins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D017434:Protein Structure, Tertiary / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000502:physiology; D014018:Tissue Distribution
D000199:Actins; D017931:DNA Primers; D018076:DNA, Complementary; D037241:Mannose-Binding Lectins; D012333:RNA, Messenger