[Comparative studies on detection of Chlamydophila psittaci and Chlamydophila abortus in meat turkey flocks using cell culture, ELISA, and PCR].
The prevalence of chlamydia in 10 meat turkey flocks was investigated. As samples served of each moment of collection and sex of the animals 10 cloacal swabs which were taken at the age of 1, 4, 8 and 12 (females) or 16 weeks (males) and at the time of slaughter at the age of 16 or 20 weeks. Spleen samples were taken at the time of slaughter, additionally. These were pooled making 1 pool out of 5 individual samples. The cloacal and spleen pools were examined by nested PCR (nPCR), Capture-ELISA and Capture Blocking-ELISA directly as well as after isolation attempts in cell cultures. The most sensitive method to detect chlamydia, with 6 isolates proved to be the isolation by cell culture followed by detection using nPCR. Not corresponding to the results of the nPCR were 4 positive reactions found by the Capture-ELISA which could in no case be affirmed by Capture-Blocking-ELISA. The direct examination of cloacal swab pools by nPCR proved positive in only 2 cases. In contrast to this the examination of these samples by Capture-ELISA showed a high percentage of 71.9% positive results, of which only 2 cases were confirmed by nPCR and none by Capture-Blocking-ELISA. Of the 8 Chlamydia positive results in the nPCR 7 could be classified by DNA sequencing to Cp. abortus and only one to Cp. psittaci.
Chemisches und Veterinäruntersuchungsamt Stuttgart, Fellbach.
R
R
Sting
E
E
Lerke
H
H
Hotzel
S
S
Jodas
C
C
Popp
H M
HM
Hafez
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D023501:Chlamydophila / Q000276:immunology / Q000302:isolation & purification; D023521:Chlamydophila Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D002691:Chlamydophila psittaci / Q000276:immunology / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis*; D009956:Psittacosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014422:Turkeys
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial