Photosensitizing action of isomeric zinc N-methylpyridylporphyrins in human carcinoma cells.
The success of photodynamic therapy (PDT), as a minimally invasive approach, in treating both neoplastic and non-neoplastic diseases has stimulated the search for new compounds with potential application in PDT. We have previously reported that Zn(II) N-alkylpyridylporphyrins (ZnTM-2(3,4)-PyP(4+) and ZnTE-2-PyP(4+)) can act as photosensitizers and kill antibiotic-resistant bacteria. This study investigated the photosensitizing effects of the isomers of ZnTMPyP(4+) (ZnTM-2(3,4)-PyP(4+)) and respective ligands on a human colon adenocarcinoma cell line. At 10 microM and 30 min of illumination the isomeric porphyrins completely inhibited cell growth, and at 20 microM killed approximately 50% of the cancer cells. All these effects were entirely light-dependent. The isomers of the ZnTMPyP(4+) and the respective ligands show high photosensitizing efficiency and no toxicity in the dark. Their efficacy as photosensitizers is comparable to that of hematoporphyrin derivative (HpD).
Al-Mutairi
Dalal A
DA
Faculty of Medicine, Kuwait University, Department of Biochemistry, Safat.
Craik
James D
JD
Batinic-Haberle
Ines
I
Benov
Ludmil T
LT
D000230:Adenocarcinoma / Q000188:drug therapy / Q000532:radiotherapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000494:pharmacology*; D020033:Protein Isoforms / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D017319:Photosensitizing Agents; D011166:Porphyrins; D020033:Protein Isoforms; D015032:Zinc