Glycoprotein (116 kD) isolated from Ulmus davidiana Nakai protects from injury of 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated BNL CL.2 cells.
Ulmus davidiana Nakai (UDN) has been used for a long time to cure inflammation in oriental medicine. To evaluate the cytoprotective effects of the UDN glycoprotein, we measured cytotoxicity, the level of intracellular reactive oxygen species (ROS), activity of nuclear factor-kappaB (NF-kappaB), nitric oxide (NO) production, and thiobarbituric acid-reactive substances (TBARS) formation in 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated BNL CL.2 cells. In TPA-treated BNL CL.2 cells, the results showed that UDN glycoprotein has dose-dependent blocking activities against TPA-induced cytotoxicity and NF-kappaB activation. In cytotoxic-related events, UDN glycoprotein (200 microg/ml) has an inhibitory effect on intracellular ROS production, NO production, and TBARS formation, without any toxic effects in the BNL CL.2 cells. These results suggest that UDN glycoprotein has cytoprotective abilities against TPA-induced oxidative cell injury.
Oh
Phil-Sun
PS
Molecular Biochemistry Laboratory, Institute of Biotechnology, Chonnam National University, 300 Yongbong-Dong, Kwangju City, 500-757, South Korea.
Lee
Sei-Jung
SJ
Lim
Kye-Taek
KT
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D007249:Inflammation / Q000188:drug therapy; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D034403:Ulmus / Q000737:chemistry*
D006023:Glycoproteins; D016328:NF-kappa B; D010940:Plant Proteins; D020011:Protective Agents; D017382:Reactive Oxygen Species; D013755:Tetradecanoylphorbol Acetate