[Studies on the effect of the adhesion protein isolated from bovine eye on cell proliferation in the newt cornea].
The effect of the adhesion protein isolated from the bovine cornea was studied on the model of mechanical injury (cross cutting of the cornea). In the concentration of 10(-12) mg/ml, the protein influenced the proliferation of corneal epithelial cells in newt Pleurodeles waltl in vivo. Experiments were conducted using autoradiography, and the nuclear labeling index (NLI) was determined at different times after surgery and in different corneal regions. This adhesion protein significantly induced proliferation of corneal epithelial cells relative to control groups with the injured eyes treated with the serum adhesion protein at the same concentration or water. The differences between the experimental and control animals were most pronounced 7 days after surgery. By day 14, they were less pronounced but still significant. On day 28, no significant differences in NLI were observed between the three groups, although these values remained higher than in intact animals. An increased pool of proliferating cells in the corneal epithelium was observed both in the affected and intact areas. The data obtained indicate that the biological activity of this protein is not species specific and that it can be a proliferation factor for corneal epithelial cells.
D V
DV
Margasiuk
E N
EN
Grigorian
V P
VP
Iamskova
D000818:Animals; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D003315:Cornea / Q000166:cytology / Q000187:drug effects*; D065306:Corneal Injuries; D019573:Epithelium, Corneal / Q000187:drug effects / Q000293:injuries; D010999:Pleurodeles; D014945:Wound Healing / Q000187:drug effects*
D015815:Cell Adhesion Molecules