Stable RNA interference in Spodoptera frugiperda cells by a DNA vector-based method.
Double-stranded RNA (dsRNA)-mediated interference (RNAi) is a powerful tool for silencing of gene expression in many organisms. To establish a DNA vector-based method for stable RNAi in Spodoptera frugiperda cells (Sf9), we created a stably transfected Sf9 cell line to express large dsRNA fragment targeting to silence the firefly luciferase gene (luc). The luc dsRNA specifically and stably suppressed the baculovirus-mediated luciferase expression. Thus, gene silencing in Sf9 cells was achieved using DNA vectors similar to the facile design described in this study.
Lin
Chih-Chien
CC
Department of Life Science and Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC.
Hsu
John T-A
JT
Huang
Kai-Ling
KL
Tang
Hung-Kuan
HK
Lai
Yiu-Kay
YK
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D004247:DNA / Q000008:administration & dosage / Q000235:genetics*; D020868:Gene Silencing / Q000502:physiology; D005822:Genetic Vectors / Q000235:genetics; D007313:Insecta; D049409:Luciferases, Firefly / Q000502:physiology*; D034622:RNA Interference / Q000502:physiology*; D018411:Spodoptera / Q000502:physiology*; D014162:Transfection / Q000379:methods*
D004247:DNA; D049409:Luciferases, Firefly