Inflammatory markers in the patients of Japanese encephalitis.
OBJECTIVE
Japanese encephalitis (JE) is one of the commonest viral encephalitis especially prevalent in Southeast Asia. Estimated mortality rate of JE is approximately 30%, with survivors undergoing severe and irreversible neurological sequelae. Although central nervous system (CNS) inflammation is imminent upon JE infection, the pathways underlying the same have not yet been clearly elucidated. However, cytokines-tumor necrosis factor-alpha (TNF-alpha) and interlukin-2 (IL-2), are small secreted proteins, which mediate and regulate immunity. Therefore, we wanted to evaluate the role, if any, of these cytokines in the pathogenesis of JE.


METHODS
We measured the levels of TNF-alpha and IL-2 in the serum and cerebrospinal fluid (CSF) of patients suffering JE, using enzyme-linked immunosorbent assay (ELISA).


RESULTS
JE infection caused a remarkable increase (p<0.0001) in the levels of TNF-alpha in the serum and CSF, while IL-2 levels were unaffected.


CONCLUSIONS
These results show that TNF-alpha pathway is involved in JE infection-triggered neuroinflammation.
Department of Neurology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Lucknow, UP 226014, India. gnageshbabu@yahoo.com
G Nagesh
GN
Babu
Jayantee
J
Kalita
U K
UK
Misra
D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D001921:Brain / Q000276:immunology / Q000503:physiopathology; D016207:Cytokines / Q000097:blood* / Q000134:cerebrospinal fluid*; D004672:Encephalitis, Japanese / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D006801:Humans; D007376:Interleukin-2 / Q000134:cerebrospinal fluid; D011237:Predictive Value of Tests; D014409:Tumor Necrosis Factor-alpha / Q000134:cerebrospinal fluid; D015854:Up-Regulation / Q000276:immunology
D015415:Biomarkers; D016207:Cytokines; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha