[The study of osteogenous differentiation of MSCs transfected with different gene].
This experiment is sought to study the contribution of different gene in osteogenous differentiation of bone marrow stromal cells (MSCs) and optimize the seed cells of bone tissue engineering. Firstly, we obtained the full length gene of BMP-2, VEGF165 and bFGF by RT-PCR, and cloned into the expression vector pcDNA3. 0. After to be transfected, the MSCs cell lines which could express each of the target protein were selected out by G418. RT-PCR and immunohistochemistry were used to confirm the exogenous gene expression in MSCs. MTT showed that almost all of the MSCs which have been transfected with exogenous gene had more strong proliferative potential than the untransfected group did. There was a notable increase of ALP activity in transfected cell compared with the control group. The concentration of OCN in cell culture medium had a increase in some degree except of VEGF group. The outstanding osteogenous differentiation could be observed in BMP-2 and bFGF transfected MSCs. The results showed that the MSCs modified by BMP-2 and bFGF would be more effective in bone tissue engineering field.
Li
Yi
Y
Institute of Biomedical Engineering, West China Medical Center of Sichuan University, Chengdu 610041, China.
Chen
Huaiqing
H
Cheng
Min
M
Tang
Yanjuan
Y
Wu
Jiang
J
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D002454:Cell Differentiation; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D019485:Bone Morphogenetic Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2