Thermophilic protease production by Streptomyces sp. 594 in submerged and solid-state fermentations using feather meal.
OBJECTIVE
Protease production by Streptomyces sp. 594 in submerged (SF) and solid-state fermentation (SSF) using feather meal, an industrial poultry residue, and partial characterization of the crude enzyme.


RESULTS
Streptomyces sp. 594 produced proteases in SF (7.2 +/- 0.2 U ml(-1)) and SSF (15.5 +/- 0.41 U g(-1)), with pH increase in both media. Considering protease activity, values obtained in the liquid extract after SSF (6.3 +/- 0.17 U ml(-1)) were lower than those from SF. The proteases, which belong to serine and metalloproteinase classes, were active over a wide range of pH (5.0-10.0) and high temperatures (55-80 degrees C). Strain 594 was also able to degrade feather in agar and liquid media. Keratinase activity (80 U l(-1)) also confirmed the keratin degrading capacity of this streptomycete.


CONCLUSIONS
Proteases produced using residues from poultry industry have shown interesting properties for industrial purposes.


CONCLUSIONS
As far as we are concerned, this is the first contribution towards the production of thermophilic protease by a streptomycete in SSF using a keratinous waste.
De Azeredo
L A I
LA
Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Federal do Rio de Janeiro, Brasil.
De Lima
M B
MB
Coelho
R R R
RR
Freire
D M G
DM
D000818:Animals; D003470:Culture Media; D005241:Feathers; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D010618:Phenanthrolines / Q000494:pharmacology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology*; D013696:Temperature
D003470:Culture Media; D010618:Phenanthrolines; D011480:Protease Inhibitors; D010664:Phenylmethylsulfonyl Fluoride; D010447:Peptide Hydrolases; C035855:keratinase