LTA recognition by bovine gammadelta T cells involves CD36.
CD36 has recently been shown to facilitate monocyte Toll-like receptor 2 (TLR2) recognition of lipoteichoic acid (LTA), much like CD14 in TLR4 recognition of lipopolysaccharide. We previously found that bovine gammadelta T cells express CD36 transcripts. Here, we tested whether bovine gammadelta T cells express CD36 protein and if so, whether it functions in a manner similar to the monocyte molecule. CD36 transcripts and internal and cell surface protein could be detected in resting, sorted gammadelta T cells. Phorbol 12-myristate 13-acetate (PMA)/ionomycin treatment increased CD36 transcript levels (detectable at 4 h) and protein expression (internal and cell surface). Increased surface antigen expression was detectable by 24 h and was maximal at 72 h following PMA/ionomycin stimulation. Anti-CD36 monoclonal antibody inhibited increased macrophage-inflammatory protein-1alpha gene expression in gammadelta T cells activated by LTA. In conclusion, gammadelta T cells express CD36, previously thought to be a myeloid and endothelial cell-restricted surface antigen, and it contributes to responses by these cells to microbial LTA.
Lubick
Kirk
K
Montana State University, Bozeman, MT 59718, USA.
Jutila
Mark A
MA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D018955:CD36 Antigens / Q000276:immunology / Q000502:physiology*; D002417:Cattle / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology / Q000494:pharmacology; D015759:Ionomycin / Q000494:pharmacology; D055666:Lipopeptides; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*; D013682:Teichoic Acids / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D051195:Toll-Like Receptor 2 / Q000037:antagonists & inhibitors / Q000502:physiology
D000911:Antibodies, Monoclonal; D018955:CD36 Antigens; D007075:Immunoglobulin M; D055666:Lipopeptides; D008070:Lipopolysaccharides; C508075:Pam(3)CSK(4) peptide; D010455:Peptides; D016692:Receptors, Antigen, T-Cell, gamma-delta; D013682:Teichoic Acids; D051195:Toll-Like Receptor 2; D015759:Ionomycin; C009900:lipoteichoic acid; D013755:Tetradecanoylphorbol Acetate