Effects of gabexate mesilate on ischemia-reperfusion-induced testicular injury in rats.
The aim of this study was to determine the effects of a synthetic serine protease inhibitor, gabexate mesilate (GM), in rats with ischemia-reperfusion (I-R) damage due to unilateral testicular torsion. Thirty male Sprague-Dawley rats were separated into three groups, each containing ten rats. A sham operation was performed in group 1 (control). In group 2 (I-R/untreated), 1 h detorsion of the testis was performed after 6 h of unilateral testicular torsion. In group 3 (I-R/GM), after performing the same surgical procedures as in group II, gabexate mesilate was given intravenously. In all experimental rats, ipsilateral orchiectomies were performed for histological examination and measuring the tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). MDA values and the testicular injury score decreased and SOD, CAT and GSH-Px values increased in the GM-treated group compared to the I-R/untreated group. The Tc-99m pertechnetate uptake ratio and the perfusion index were significantly decreased in the group 2 compared to the group 1 and 3 rats. In group 3, these values were significantly increased compared to group 2. Most of the specimens in the GM-treated group showed grade-I testicular injury. However, the injuries in the I-R/untreated rats varied between grade-III and grade-IV. The results of this study show that GM may play a role in reducing the injury caused by I-R.
Department of Nuclear Medicine, Dicle University, Medical School, Diyarbakir, Turkey.
Ayten
A
Gezici
Hayrettin
H
Ozturk
Huseyin
H
Buyukbayram
Hulya
H
Ozturk
Hanifi
H
Okur
D000818:Animals; D016670:Gabexate / Q000627:therapeutic use*; D015227:Lipid Peroxidation; D008297:Male; D008833:Microcirculation; D011877:Radionuclide Imaging; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D015842:Serine Proteinase Inhibitors / Q000627:therapeutic use*; D013086:Spermatic Cord Torsion / Q000150:complications; D013737:Testis / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000473:pathology*
D015842:Serine Proteinase Inhibitors; D016670:Gabexate