New caerin antibiotic peptides from the skin secretion of the Dainty Green Tree Frog Litoria gracilenta. Identification using positive and negative ion electrospray mass spectrometry.
The skin secretion of the Dainty Green Tree Frog Litoria gracilenta contains 16 peptides, which protect the animal from predators, both large and small. A combination of negative and positive ion electrospray mass spectrometry together with Lys-C enzymic digest and Edman sequencing identifies three new wide-spectrum caerin 1 antibiotics, namely Caerin 1.17 [GLFSVLGSVAKHLLPHVAPIIAEKL-NH2], Caerin 1.18 [GLFSVLGSVAKHLLPHVVPVIAEKL-NH2], and Caerin 1.19 [GLFKVLGSVAKHLLPHVAPIIAEKL-NH2], and a narrow spectrum antibiotic Caerin 3.5 [GLWEKVKEKANELVSGIVEGVK-NH2].
Department of Chemistry, The University of Adelaide, Adelaide, SA 5005, Australia.
Micheal J
MJ
Maclean
Craig S
CS
Brinkworth
Daniel
D
Bilusich
John H
JH
Bowie
Jason R
JR
Doyle
Lyndon E
LE
Llewellyn
Michael J
MJ
Tyler
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry*; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D001001:Anura; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D012867:Skin / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides