Dynamics of amino acid metabolism of primary human liver cells in 3D bioreactors.
The kinetics of 18 amino acids, ammonia (NH3) and urea (UREA) in 18 liver cell bioreactor runs were analyzed and simulated by a two-compartment model consisting of a system of 42 differential equations. The model parameters, most of them representing enzymatic activities, were identified and their values discussed with respect to the different liver cell bioreactor performance levels. The nitrogen balance based model was used as a tool to quantify the variability of runs and to describe different kinetic patterns of the amino acid metabolism, in particular with respect to glutamate (GLU) and aspartate (ASP).
Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute, Beutenbergstr. 11a, 07745, Jena, Germany. Reinhard.Guthke@hki-jena.de
R
R
Guthke
K
K
Zeilinger
S
S
Sickinger
W
W
Schmidt-Heck
H
H
Buentemeyer
K
K
Iding
J
J
Lehmann
M
M
Pfaff
G
G
Pless
J C
JC
Gerlach
D000596:Amino Acids / Q000378:metabolism*; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003198:Computer Simulation; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D019164:Liver, Artificial; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D023822:Tissue Engineering / Q000379:methods*
D000596:Amino Acids