No evidence of population structure across three isolated subpopulations of Russula brevipes in an oak/pine woodland.
Russula brevipes is common ectomycorrhizal (EM) fungus that is associated with several hosts across temperate forest ecosystems. A previous study has demonstrated that substructuring across large geographic distances (1500 km) occurs in the western USA. To examine genetic structure over a more localized scale, basidiocarps of Russula brevipes from three subpopulations, separated by distances of 230-1090 m, were collected over two consecutive years in an oak/pine woodland. Microsatellite loci were used to test for population differentiation both among subpopulations and by year. No significant population differentiation was detected between subpopulations (theta(ST) = 0.01) or between years (theta(ST) = 0.01). Most loci were consistent with a Hardy-Weinburg equilibrium and 82% of the genets between seasons from similar sampling localities constituted new genotypes. These results indicate that R. brevipes constitutes a randomly mating population with no genetic differentiation between locations or across successive fruiting seasons.
Bergemann
Sarah E
SE
University of California, Berkeley, Department of Environmental Science, Policy and Management, Division of Ecosystem Science, 137 Mulford Hall #3114, Berkeley, CA 94720, USA. sbergemann@nature.berkeley.edu
Douhan
Greg W
GW
Garbelotto
Matteo
M
Miller
Steven L
SL
D000363:Agaricales / Q000145:classification / Q000235:genetics*; D000483:Alleles; D000704:Analysis of Variance; D051456:Gene Flow; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D005843:Geography; D018895:Microsatellite Repeats / Q000235:genetics; D028223:Pinus / Q000382:microbiology*; D029963:Quercus / Q000382:microbiology*; D012098:Reproduction; D014197:Trees