Enzyme stabilizer DTT catalyzes nitrilase analogue hydrolysis of nitriles.
Amides are the dominating products in some nitrilase catalyzed conversions of alpha-activated nitriles, but unexpectedly this hydrolytic reaction is also catalyzed by 1,4-dithio-dl-threitol (DTT), a standard antioxidizing enzyme stabilizer.
Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 16/II, A-8010 Graz, Austria.
Margit
M
Winkler
Anton
A
Glieder
Norbert
N
Klempier
D000577:Amides / Q000737:chemistry*; D000619:Aminohydrolases / Q000737:chemistry*; D002384:Catalysis; D004229:Dithiothreitol / Q000737:chemistry*; D004795:Enzyme Stability; D006868:Hydrolysis; D015394:Molecular Structure; D009570:Nitriles / Q000737:chemistry*
D000577:Amides; D009570:Nitriles; D000619:Aminohydrolases; C021867:nitrilase; D004229:Dithiothreitol