Cell-cell interaction-dependent regulation of N-acetylglucosaminyltransferase III and the bisected N-glycans in GE11 epithelial cells. Involvement of E-cadherin-mediated cell adhesion.
Changes in oligosaccharide structures are associated with numerous physiological and pathological events. In this study, the effects of cell-cell interactions on N-linked oligosaccharides (N-glycans) were investigated in GE11 epithelial cells. N-glycans were purified from whole cell lysates by hydrazinolysis and then detected by high performance liquid chromatography and mass spectrometry. Interestingly, the population of the bisecting GlcNAc-containing N-glycans, the formation of which is catalyzed by N-acetylglucosaminyltransferase III (GnT-III), was substantially increased in cells cultured under dense conditions compared with those cultured under sparse conditions. The expression levels and activities of GnT-III but not other glycosyltransferases, such as GnT-V and alpha1,6-fucosyltransferase, were also consistently increased in these cells. However, this was not observed in mouse embryonic fibroblasts or MDA-MB231 cells, in which E-cadherin is deficient. In contrast, perturbation of E-cadherin-mediated adhesion by treatment with EDTA or a neutralizing anti-E-cadherin antibody abolished the up-regulation of expression of GnT-III. Furthermore, we observed the significant increase in GnT-III activity under dense growth conditions after restoration of the expression of E-cadherin in MDA-MB231 cells. Our data together indicate that a E-cadherin-dependent pathway plays a critical role in regulation of GnT-III expression. Given the importance of GnT-III and the dynamic regulation of cell-cell interaction during tissue development and homeostasis, the changes in GnT-III expression presumably contribute to intracellular signaling transduction during such processes.
Iijima
Junko
J
Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
Zhao
Yanyang
Y
Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
Isaji
Tomoya
T
Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan; Division of Regulatory Glycobiology, Tohoku Pharmaceutical University, Sendai, Miyagi 981-8558, Japan, and the.
Kameyama
Akihiko
A
Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8568, Japan.
Nakaya
Shuuichi
S
Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8568, Japan.
Wang
Xiangchun
X
Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
Ihara
Hideyuki
H
Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
Cheng
Xinyao
X
Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
Nakagawa
Takatoshi
T
Department of Glycotherapeutics, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
Miyoshi
Eiji
E
Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
Kondo
Akihiro
A
Department of Glycotherapeutics, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
Narimatsu
Hisashi
H
Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8568, Japan.
Taniguchi
Naoyuki
N
Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan. Electronic address: proftani@biochem.med.osaka-u.ac.jp.
Gu
Jianguo
J
Department of Biochemistry, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan; Division of Regulatory Glycobiology, Tohoku Pharmaceutical University, Sendai, Miyagi 981-8558, Japan, and the. Electronic address: jgu@tohoku-pharm.ac.jp.
D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism*; D005347:Fibroblasts; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D015398:Signal Transduction; D015854:Up-Regulation
D015820:Cadherins; D011134:Polysaccharides; D017351:N-Acetylglucosaminyltransferases; C054599:beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase