Active immunization against Pneumocystis carinii with a recombinant P. carinii antigen.
Mice immunized with recombinant mouse Pneumocystis carinii antigen A12-thioredoxin fusion protein developed an antibody response that recognized P. carinii antigens, as determined by Western blotting and immunofluorescence analysis. Compared to mice immunized with thioredoxin alone, mice immunized with A12-thioredoxin had significantly reduced lung P. carinii burdens after CD4+ T-cell depletion and challenge with P. carinii.
Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Box 690, 601 Elmwood Ave., Rochester, NY 14642, USA.
Jesse
J
Wells
Constantine G
CG
Haidaris
Terry W
TW
Wright
Francis
F
Gigliotti
D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis / Q000097:blood; D000946:Antigens, Fungal / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D045363:Pneumocystis carinii / Q000276:immunology*; D011020:Pneumonia, Pneumocystis / Q000276:immunology / Q000517:prevention & control*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000737:chemistry; D013879:Thioredoxins / Q000008:administration & dosage / Q000276:immunology
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D011993:Recombinant Fusion Proteins; D013879:Thioredoxins; C470926:Kex1 protein, Pneumocystis jirovecii; D012697:Serine Endopeptidases