Rapid and highly sensitive liquid chromatography/electrospray ionization tandem mass spectrometry method for the quantitation of buspirone in human plasma: application to a pharmacokinetic study.
A rapid and sensitive method for the quantitation of buspirone in human plasma by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was developed. Plasma samples were treated by liquid-liquid extraction with methyl tert-butyl ether (MTBE). The chromatographic separation was performed isocratically on a reversed-phase Shiseido C18 column (50 mm x 2.0 mm, 3 microm) with a mobile phase of acetonitrile/0.1% acetic acid (1:1, v/v). The acquisition was performed in multiple reaction monitoring (MRM) mode, monitoring the transitions m/z 386 --> 122 for buspirone and m/z 409 --> 238 for amlodipine (the internal standard). The method was validated to determine its specificity, recovery, limit of quantitation, accuracy and precision. The lower limit of quantitation was 0.02 ng/mL with a relative standard deviation of less than 10%. The present method provides an accurate, precise and sensitive tool for buspirone and was successfully applied to a pharmacokinetic study in eight subjects.
College of Pharmacy, Kyung-Hee University, Hoegi-dong, Dongdaemun-ku, Seoul 130-701, Korea.
Sung-Hee
SH
Cho
Heon-Woo
HW
Lee
Ho-Taek
HT
Im
Wan-Su
WS
Park
Young-Wook
YW
Choi
Jae-Hwan
JH
Rew
Kyung-Tae
KT
Lee
D000328:Adult; D017311:Amlodipine / Q000097:blood; D014151:Anti-Anxiety Agents / Q000097:blood* / Q000493:pharmacokinetics*; D019540:Area Under Curve; D002065:Buspirone / Q000097:blood* / Q000493:pharmacokinetics*; D002121:Calcium Channel Blockers / Q000097:blood; D002138:Calibration; D002853:Chromatography, Liquid; D006207:Half-Life; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D014151:Anti-Anxiety Agents; D002121:Calcium Channel Blockers; D007202:Indicators and Reagents; D012997:Solvents; D017311:Amlodipine; D002065:Buspirone