Multilineage engraftment of refrozen cord blood hematopoietic progenitors in NOD/SCID mice.
Seven cord blood (CB) units were tested for their capacity to repopulate irradiated NOD/SCID mice after one or two successive cryopreservation procedures. In primary transplants with frozen or refrozen CB cells we observed equivalent human colonies and percentages of human CD45+ cells, with multilineage engraftment. In secondary transplants flow cytometry and polymerase chain reaction for the a satellite region of chromosome 17 showed equivalent levels of human engraftment. Since CB units have, to date, mainly been stored in individual bags, our results suggest new options for optimizing the timing of infusions of expanded and non-expanded progenitors in transplants.
Timeus
Fabio
F
Paediatric Haematology-Oncology Department, University of Torino, Italy. fabio.timeus@unito.it
Crescenzio
Nicoletta
N
Sanavio
Fiorella
F
Fazio
Luigina
L
Doria
Alessandra
A
Foglia
Luiselda
L
Pignochino
Ymera
Y
Berger
Massimo
M
Piacibello
Wanda
W
Madon
Enrico
E
Cordero di Montezemolo
Luca
L
Fagioli
Franca
F
D000818:Animals; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D005312:Fetal Blood / Q000637:transplantation*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D013045:Species Specificity