Prediction of mono- and polycyclic aromatic hydrocarbon degradation in spiked soils using cyclodextrin extraction.
In this study, an aqueous-based hydroxypropyl-beta-cyclodextrin (HPCD) extraction technique was assessed for its capacity to determine the microbially degradable fraction of mono- and polycyclic aromatic hydrocarbons in four dissimilar soils. A linear relationship (slope=0.90; R2=0.89), approaching 1:1 between predicted and observed phenanthrene mineralization, was demonstrated for the cyclodextrin extraction; however, the water only extraction underestimated the microbially available fraction by a factor of three (slope=3.35; R2=0.64). With respect to determining the mineralizable fraction of p-cresol in soils, the cyclodextrin extraction (slope=0.94; R2=0.84) was more appropriate than the water extraction (slope=1.50; R2=0.36). Collectively, these results suggested that the cyclodextrin extraction technique was suitable for the prediction of the mineralizable fraction of representative PAHs and phenols present in dissimilar soils following increasing soil-contaminant contact times. The assessment of the microbial availability of contaminants in soils is important for a more representative evaluation of soil contamination.
School of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
Ian J
IJ
Allan
Kirk T
KT
Semple
Rina
R
Hare
Brian J
BJ
Reid
D002250:Carbon Radioisotopes; D003408:Cresols / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D010616:Phenanthrenes / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry*; D013997:Time Factors
D002250:Carbon Radioisotopes; D003408:Cresols; D003505:Cyclodextrins; D010616:Phenanthrenes; D012989:Soil Pollutants; C031181:phenanthrene; C077977:cresol