Tyrosinase inhibitory activity of citrus essential oils.
Thirteen kinds of citrus essential oils and their volatile flavor constituents were investigated for tyrosinase inhibitory activity. Eureka lemon, Lisbon lemon, Keraji, and Kiyookadaidai significantly inhibited the oxidation of L-dihydroxy phenylalanine (L-DOPA) by mushroom tyrosinase. Citral and myrcene among volatile flavor constituents of citrus essential oils exhibited tyrosinase inhibitory activities with Ki values of 0.318 and 2.38 mM, respectively. The inhibition kinetics analyzed by a Lineweaver-Burk plot indicated that citral is a noncompetitive inhibitor and myrcene is a competitive inhibitor. These results indicated that citral and myrcene are responsible for the tyrosinase inhibitory activity of citrus essential oils.
Department of Bioresources Science, Faculty of Agriculture, Kochi University, Mononbe B-200, Nankoku 783-8502, Japan.
Ritaro
R
Matsuura
Hiroyuki
H
Ukeda
Masayoshi
M
Sawamura
D000080462:Acyclic Monoterpenes; D000475:Alkenes / Q000494:pharmacology; D002957:Citrus / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D007700:Kinetics; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D039821:Monoterpenes / Q000494:pharmacology; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D013649:Taste; D014835:Volatilization
D000080462:Acyclic Monoterpenes; D000475:Alkenes; D004791:Enzyme Inhibitors; D039821:Monoterpenes; D009822:Oils, Volatile; C509595:myrcene; D014442:Monophenol Monooxygenase; C007076:citral