Subcellular Localization of Salmonella enteritidis Endotoxin in Liver and Spleen of Mice and Rats.
Salmonella enteritidis(14)C-endotoxin was recovered predominantly from the nuclear and mitochondrial subcellular fractions of livers and spleens of mice and rats, 3.5 hr and 3 days after intravenous administration. Of the recovered radioactivity, 10 to 20% was present in the liver mitochondrial fraction as high-molecular-weight, biologically active material, suggesting the presence of intact endotoxin. Autoradiographic studies demonstrated nuclear and cytoplasmic labeling in the liver and at least nuclear label in spleen cells. The resistance of rats, as compared to mice, to the induction of amyloidosis does not appear to be based on a difference in subcellular localization of endotoxin within the reticuloendothelial system.
Arthritis and Rheumatism Branch and the Laboratory of Biochemical Pharmacology of the National Institute of Arthritis and Metabolic Diseases, the Laboratory of Viral Diseases and the Laboratory of Clinical Investigation of the National Institute of Allergy and Infectious Diseases, and the Ophthalmology Branch of the National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20014.
J T
JT
Willerson
R L
RL
Trelstad
T
T
Pincus
S B
SB
Levy
S M
SM
Wolff