beta1,4-N-Acetylglucosaminyltransferase III potentiates beta1 integrin-mediated neuritogenesis induced by serum deprivation in Neuro2a cells.
Aspects of the biological significance of the bisecting N-acetylglucosamine (GlcNAc) structure on N-glycans introduced by beta1,4-N-acetylglucosaminyltransferase III (GnT-III) in Neuro2a cell differentiation are demonstrated. The overexpression of GnT-III in the cells led to the induction of axon-like processes with numerous neurites and swellings, in which beta1 integrin was localized, under conditions of serum starvation. This enhancement in neuritogenesis was suppressed by either the addition of a bisecting GlcNAc-containing N-glycan or erythroagglutinating phytohemagglutinin (E(4)-PHA), which preferentially recognizes the bisecting GlcNAc. GnT-III-promoted neuritogenesis was also significantly perturbed by treatment with a functional blocking anti-beta1 integrin antibody. In fact, beta1 integrin was found to be one of the target proteins of GnT-III, as confirmed by a pull-down assay with E(4)-PHA. These data suggest that N-glycans with a bisecting GlcNAc on target molecules, such as beta1 integrin, play important roles in the regulation of neuritogenesis.
Shigeta
Masaki
M
Department of Biochemistry, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
Shibukawa
Yukinao
Y
Ihara
Hideyuki
H
Miyoshi
Eiji
E
Taniguchi
Naoyuki
N
Gu
Jianguo
J
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D016895:Culture Media, Serum-Free; D006031:Glycosylation; D019012:Integrin beta1 / Q000502:physiology*; D051379:Mice; D017351:N-Acetylglucosaminyltransferases / Q000502:physiology*; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000166:cytology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding
D016895:Culture Media, Serum-Free; D019012:Integrin beta1; D010835:Phytohemagglutinins; D011134:Polysaccharides; C067494:erythroagglutinating phytohemagglutinin; D017351:N-Acetylglucosaminyltransferases; C054599:beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase