LDL-antioxidant pterocarpans from roots of Glycine max (L.) Merr.
The methanolic root extract of Glycine max (L.) Merr. was chromatographed, which yielded 10 flavonoids, including three isoflavones 1-3, five pterocarpans 4-8, one flavonol 9, and one anthocyanidin 10. All isolated compounds were examined for LDL-antioxidant activities using four different assay systems on the basis of Cu2+-mediated oxidation. Among them, seven compounds showed potent LDL-antioxidant activities in the thiobarbituric acid reactive substances (TBARS) assay, the lag time of conjugated diene formation, relative electrophoretic mobility (REM), and fragmentation of apoB-100 on copper-mediated LDL oxidation. Three pterocarpans 4, 6, and 7, never reported as LDL-antioxidant, showed potent activities with IC50 values of 19.8, 0.9, 45.0 microM, respectively, in comparison with probucol (IC50 = 5.6 microM) as positive control. Interestingly, coumestrol 6 (IC50 = 0.9 microM) showed 20 times more activity in the TBARS assay than genistein (IC50 = 30.1 microM) and daidzein (IC50 = 21.6 microM), representative antioxidants in soybean. Moreover, coumestrol 6 had an extended lag time of 190 min at 3.0 microM in measuring conjugated diene formation, while both genistein (120 min) and daidzein (93 min) lag times were extended to less than 120 min at the same concentration.
Division of Applied Life Science (BK21 Program), Department of Agricultural Chemistry, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Korea.
Jin Hwan
JH
Lee
Byong Won
BW
Lee
Jin Hyo
JH
Kim
Tae-Sook
TS
Jeong
Min Jung
MJ
Kim
Woo Song
WS
Lee
Ki Hun
KH
Park
D003300:Copper / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D036343:Pterocarpans / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D008077:Lipoproteins, LDL; D010936:Plant Extracts; D036343:Pterocarpans; D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper