Rapid and simultaneous determination of nonsteroidal anti-inflammatory drugs in human plasma by LC-MS with solid-phase extraction.
A rapid, selective and sensitive analytical method for the simultaneous determination of 16 nonsteroidal anti-inflammatory drugs (NSAIDs) in human plasma was carried out using Oasis HLB solid-phase extraction (SPE), followed by reversed-phase high-performance liquid chromatography and quadrupole mass spectrometry with electrospray ionization operated in the negative ion mode. The recoveries of NSAIDs from human plasma by SPE were greater than 76.7%. The use of a short column packed with small particles enabled rapid and simultaneous determination within 7 min. The detection limits for the NSAIDs were 0.01-0.9 mug/ml using the selected ion monitoring mode.
Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan. c18871@pref.gifu.lg.jp
Koichi
K
Suenami
Lee Wah
LW
Lim
Toyohide
T
Takeuchi
Yasuhide
Y
Sasajima
Kiyohito
K
Sato
Yuji
Y
Takekoshi
Susumu
S
Kanno
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000097:blood*; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000592:standards; D006801:Humans; D007477:Ions; D013058:Mass Spectrometry; D008956:Models, Chemical; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007477:Ions