Soluble triggering receptor expressed on myeloid cells (sTREM-1): a new mediator involved in the pathogenesis of peptic ulcer disease.
OBJECTIVE
Triggering receptor expressed on myeloid cells (TREM-1) is a promoter of cytokine production triggered by microbial components. To investigate the significance of its soluble counterpart, sTREM-1, for the pathogenesis of peptic ulcer disease, sTREM-1 was compared with the proinflammatory mediators and the pathology score of gastritis.


METHODS
Forty patients with dyspepsia were enrolled: 20 with peptic ulcer and 20 controls without any macroscopic abnormalities. All patients were examined by endoscopy; gastric juice was aspirated and biopsy specimens were collected from the antrum and corpus of the stomach. sTREM-1 was estimated by a hand-made enzyme immunoassay. Interleukin-8 was estimated by enzyme-linked immunosorbent assay and lipid peroxidation, indexed by malondialdehyde, by the thiobarbituric assay, after passage through a high-performance liquid chromatography system.


RESULTS
The median (+/-SE) of sTREM-1 of controls and patients with ulcer was 3.91+/-0.57 and 44.27+/-241.55 RU, respectively (P=0.006). The median (+/-SE) of interleukin-8 of controls and patients with ulcer was 1802.97+/-122.10 and 2030.66+/-64.44 pg/ml, respectively (P=0.023). sTREM-1 was positively correlated with the density of neutrophil and mononuclear infiltration scores and the total Sydney score (P=0.029, 0.043 and 0.041, respectively). sTREM-1 was positively correlated with interleukin-8 (P=0.042).


CONCLUSIONS
sTREM-1 might be an independent factor involving with the peptic ulcerative inflammatory process that is positively correlated with histopathological abnormalities of gastritis.
Department of Gastroenterology, Sismanoglion General Hospital of Athens, Greece. basilkoussoulas3@hotmail.com
Vassilios
V
Koussoulas
Spyridon
S
Vassiliou
Maria
M
Demonakou
Georgios
G
Tassias
Evangelos J
EJ
Giamarellos-Bourboulis
Maria
M
Mouktaroudi
Helen
H
Giamarellou
Charalambos
C
Barbatzas
D016207:Cytokines / Q000097:blood; D004381:Duodenal Ulcer / Q000097:blood / Q000503:physiopathology*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000097:blood; D007413:Intestinal Mucosa / Q000503:physiopathology; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008562:Membrane Glycoproteins / Q000097:blood / Q000378:metabolism / Q000502:physiology; D008875:Middle Aged; D022423:Myeloid Cells / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000097:blood / Q000378:metabolism / Q000502:physiology; D018709:Statistics, Nonparametric; D013276:Stomach Ulcer / Q000097:blood / Q000503:physiopathology*; D000073871:Triggering Receptor Expressed on Myeloid Cells-1
D016207:Cytokines; D016209:Interleukin-8; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; C408842:TREM1 protein, human; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D008315:Malondialdehyde