On the role of stereo-electronic effects in tuning the selectivity and rate of DNA alkylation by duocarmycins.
The role of local geometric and stereo-electronic effects in tuning the alkylation of DNA by duocarmycins has been analyzed by an integrated computational tool rooted in the density functional theory and the polarizable continuum model. Our study points out that together with steric accessibility, different electronic delocalisations also contribute to determine the higher reactivity of adenine with respect to guanine. Also the effect of the methyl ester group on the alkylating agent has an electronic origin. Furthermore, deviations from the planarity in the drug structure (conformational catalysis) could be less important than currently accepted since, according to our computations, compounds with strongly different reactivity have nearly constant and very similar out of plane distortions before and after the reaction. Model computations suggest, instead, that specific non covalent interactions could discriminate between different drugs selectively reducing some activation energies with respect to the corresponding processes in solution.
Dipartimento di Chimica, Università Federico II, Complesso Universitario Monte S. Angelo, via Cintia, I-80126, Napoli, Italy.
Paola
P
Cimino
Giuseppe
G
Bifulco
Raffaele
R
Riccio
Luigi
L
Gomez-Paloma
Vincenzo
V
Barone
D000225:Adenine / Q000737:chemistry; D000478:Alkylation; D004247:DNA / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D011760:Pyrrolidinones / Q000737:chemistry*
D007211:Indoles; D011760:Pyrrolidinones; D004247:DNA; D000225:Adenine