Sensitivity of Types 1 and 2 Herpes Simplex Virus to 5-Iodo-2'-Deoxyuridine and 9-beta-d-Arabinofuranosyladenine.
The sensitivities of 21 strains of herpes simplex virus (HSV), 13 type 1 strains and 8 type 2 strains, to 5-iodo-2'-deoxyuridine (IUdR) and 9-beta-d-arabinofuranosyladenine (ara-A) were evaluated by the plaque-suppression test in chick embryo fibroblast (CEF), WI-38, and HeLa cell cultures. In CEF, type 1 strains were considerably more sensitive to the inhibitors than were the type 2 strains. In WI-38, the type 1 strains were more sensitive than the type 2 strains to IUdR; however, the two serotypes were equally sensitive to ara-A. In HeLa cells, the differences in sensitivity to IUdR between the two serotypes were less. Eight HeLa-adapted strains (four type 1 and four type 2) evaluated in HeLa cell cultures were equally sensitive to IUdR; the type 2 strains were slightly more sensitive than type 1 strains to ara-A. These results demonstrate the wide variation in sensitivity of HSV types 1 and 2 to antiviral agents which results from differences in the cell culture system and passage history of the strains.
Mayo Graduate School of Medicine (University of Minnesota), and Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55901.
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Person
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Sheridan
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Herrmann