[Determination of triterpene delta-amyrone in Sedum sarmentosum].
OBJECTIVE
To establish the quantitative analysis method of triterpene delta-amyrone in Sedum sarmentosum by HPLC-ELSD.


METHODS
The Zorbax Sil Column was used and the mobile phase was dichloromethane-methanol (99:1). The flow rate was set at 0.5 mL x min(-1), the temperature of drift tube was set at 100 degrees C and the nebulizer nitrogen flow rate was 3.0 L x min(-1).


RESULTS
The linear range of sigma-amyrone was 2.12-10.6 microg. The average recovery was 100.5%.


CONCLUSIONS
The results showed that the method was reliable, simple and precise. It would be beneficial for the study of the quality control of S. sarmentosum.
Department of Chinese Pharmceutical Analysis, China Pharmaceutical University, Nanjing.
Su-ping
SP
Cao
Li-fang
LF
Liu
Qiang
Q
Wang
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D031963:Sedum / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis*
D014315:Triterpenes; C511312:amyrone