Analysis of the molecular interaction of glycosylated proteins with rabbit liver asialoglycoprotein receptors using surface plasmon resonance spectroscopy.
A sensitive, accurate, and efficient biosensor analysis using surface plasmon resonance (SPR) spectroscopy was used for delineating the molecular interaction between rabbit liver asialoglycoprotein receptors (ASGPR) and glycosylated proteins. Isolated rabbit liver ASGPR obtained by affinity column chromatography was dissolved in buffer solution containing TritonX-100 and immobilized on the SPR sensor chip by amine coupling. The SPR study demonstrated that the association rate constants (ka) of galactosylated proteins with ASGPR are dependent on the number of galactose residues, while the dissociation rate constants (kd) are influenced not only by the surface density of the galactose moieties but also by their steric configuration. In addition, it was demonstrated that D-fucosylated BSA had a higher binding affinity to ASGPR than Gal-BSA, when the degree of sugar modification was equivalent.
Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
Takeshi
T
Terada
Makiya
M
Nishikawa
Fumiyoshi
F
Yamashita
Mitsuru
M
Hashida
D000818:Animals; D037263:Asialoglycoprotein Receptor / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D008099:Liver / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D020349:Surface Plasmon Resonance / Q000379:methods*
D037263:Asialoglycoprotein Receptor; D011506:Proteins