Differential expression of vitreous proteins in proliferative diabetic retinopathy.
OBJECTIVE
To identify vitreous proteins that were differentially expressed in patients suffering from proliferative diabetic retinopathy with active neovascularization.


METHODS
The vitreous samples of 15 active proliferative diabetic retinopathy patients were analyzed by two-dimensional gel electrophoresis and mass spectrometry, and the results were compared with those from age-matched patients with macular hole.


RESULTS
Twenty-five protein spots were identified in the two-dimensional gel electrophoresis gels. Eight proteins (pigmented epithelium derived factor, serine protease inhibitor, apolipoprotein A-IV precursor, prostaglandin-H2 D-isomerase, a(1)-antitrypsin precursor, ankyrin repeat domain 15 protein, alpha2-HS-glycoprotein, and beta V spectrin) in the 25 spots were expressed significantly differently between the macular hole and proliferative diabetic retinopathy patients (p value < 0.05). Five proteins were upregulated in the proliferative diabetic retinopathy patients, and three were downregulated (p value < 0.05).


CONCLUSIONS
We constructed vitreous protein profiles for proliferative diabetic retinopathy patients and identified eight candidate proteins believed to be involved in the pathogenesis of proliferative diabetic retinopathy.
Department of Ophthalmology, Seoul National University College of Medicine, Yongon-Dong, Korea.
Sang Jin
SJ
Kim
Sangmi
S
Kim
Jungeun
J
Park
Hong Kyu
HK
Lee
Kyong Soo
KS
Park
Hyeong Gon
HG
Yu
Youngsoo
Y
Kim
D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D003930:Diabetic Retinopathy / Q000150:complications / Q000378:metabolism* / Q000601:surgery; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D015861:Retinal Neovascularization / Q000150:complications / Q000378:metabolism / Q000601:surgery; D014821:Vitrectomy; D014822:Vitreous Body / Q000378:metabolism*
D015415:Biomarkers; D005136:Eye Proteins