High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats.
Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.
Regina
Ahmed
A
Commonwealth Scientific and Industrial Research Organization, Food Futures National Research Flagship, P.O. Box 93, North Ryde 1670, NSW, Australia.
Bird
Anthony
A
Topping
David
D
Bowden
Sarah
S
Freeman
Judy
J
Barsby
Tina
T
Kosar-Hashemi
Behjat
B
Li
Zhongyi
Z
Rahman
Sadequr
S
Morell
Matthew
M
D015061:1,4-alpha-Glucan Branching Enzyme / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000688:Amylose / Q000378:metabolism*; D000818:Animals; D004032:Diet; D030841:Food, Genetically Modified; D005818:Genetic Engineering; D005936:Glucans / Q000737:chemistry; D006304:Health Status; D006801:Humans; D007420:Intestine, Large / Q000378:metabolism; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008297:Male; D030821:Plants, Genetically Modified; D034622:RNA Interference; D051381:Rats; D017207:Rats, Sprague-Dawley; D013213:Starch / Q000737:chemistry / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000378:metabolism*
D005936:Glucans; D007527:Isoenzymes; D013213:Starch; D000688:Amylose; D015061:1,4-alpha-Glucan Branching Enzyme