Thiol-specific and nonspecific interactions between DNA and gold nanoparticles.
The contribution of nonspecific interactions to the overall interactions of thiol-ssDNA and dsDNA macromolecules with gold nanoparticles was investigated. A systematic investigation utilizing dynamic light scattering and cryogenic transmission electron microscopy has been performed to directly measure and visualize the changes in particle size and appearance during functionalization of gold nanoparticles with thiol-ssDNA and nonthiolated dsDNA. The results show that both thiol-ssDNA and dsDNA do stabilize gold nanoparticle dispersions, but possible nonspecific interactions between the hydrophobic DNA bases and the gold surface promote interparticle interactions and cause aggregation within rather a short period of time. We also discuss the adsorption mechanisms of dsDNA and thiol-ssDNA to gold particles.
Physical Chemistry 1, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden.
Marité
M
Cárdenas
Justas
J
Barauskas
Karin
K
Schillén
Jennifer L
JL
Brennan
Mathias
M
Brust
Tommy
T
Nylander
D000327:Adsorption; D004277:DNA, Single-Stranded / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D053768:Metal Nanoparticles / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D004277:DNA, Single-Stranded; D013438:Sulfhydryl Compounds