The structure of SENP1-SUMO-2 complex suggests a structural basis for discrimination between SUMO paralogues during processing.
The SUMO (small ubiquitin-like modifier)-specific protease SENP1 (sentrin-specific protease 1) can process the three forms of SUMO to their mature forms and deconjugate SUMO from modified substrates. It has been demonstrated previously that SENP1 processed SUMO-1 more efficiently than SUMO-2, but displayed little difference in its ability to deconjugate the different SUMO paralogues from modified substrates. To determine the basis for this substrate specificity, we have determined the crystal structure of SENP1 in isolation and in a transition-state complex with SUMO-2. The interface between SUMO-2 and SENP1 has a relatively poor complementarity, and most of the recognition is determined by interaction between the conserved C-terminus of SUMO-2 and the cleft in the protease. Although SENP1 is rather similar in structure to the related protease SENP2, these proteases have different SUMO-processing activities. Electrostatic analysis of SENP1 in the region where the C-terminal peptide, removed during maturation, would project indicates that it is the electrostatic complementarity between this region of SENP1 and the C-terminal peptides of the various SUMO paralogues that mediates selectivity.
Centre for Biomolecular Sciences, University of St. Andrews, St. Andrews, Scotland KY16 9ST, UK.
Lin Nan
LN
Shen
Changjiang
C
Dong
Huanting
H
Liu
James H
JH
Naismith
Ronald T
RT
Hay
D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D025841:Small Ubiquitin-Related Modifier Proteins / Q000737:chemistry / Q000502:physiology*; D055672:Static Electricity; D013379:Substrate Specificity
C117909:SUMO2 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D010450:Endopeptidases; C404512:SENP1 protein, human; D003546:Cysteine Endopeptidases