Post-translational control of the Streptomyces lividans ClgR regulon by ClpP.
It has been shown previously that expression of the Streptomyces lividans clpP1P2 operon, encoding proteolytic subunits of the Clp complex, the clpC1 gene, encoding the ATPase subunit, and the lon gene, encoding another ATP-dependent protease, are all activated by ClgR. The ClgR regulon also includes the clgR gene itself. It is shown here that the degradation of ClgR and Lon is ClpP1/P2-dependent and that the two C-terminal alanines of these new substrates are involved in their stability. The ClpC1 protein, which does not end with two alanines, is also accumulated in a clpP1P2 mutant. The results presented here support the idea that ClpP1/P2 ensure post-translational control of ClgR regulon members, including ClgR itself.
Unité Postulante de Génétique Bactérienne et Différenciation, CNRS URA 2172, Institut Pasteur, 25 rue Dr Roux, 75724 Paris Cedex 15, France.
Audrey
A
Bellier
Unité Postulante de Génétique Bactérienne et Différenciation, CNRS URA 2172, Institut Pasteur, 25 rue Dr Roux, 75724 Paris Cedex 15, France.
Myriam
M
Gominet
Unité Postulante de Génétique Bactérienne et Différenciation, CNRS URA 2172, Institut Pasteur, 25 rue Dr Roux, 75724 Paris Cedex 15, France.
Philippe
P
Mazodier
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D049071:Endopeptidase Clp / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D049070:Protease La / Q000378:metabolism*; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D018085:Regulon; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048170:Streptomyces lividans / Q000201:enzymology / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D015534:Trans-Activators; D049070:Protease La; D049071:Endopeptidase Clp