The identification of matrix metalloproteinases and their tissue inhibitors in broiler chickens by immunohistochemistry.
The aim of this study was to investigate the distribution of matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TIMP-2 using immunohistochemistry in the ascites syndrome of broiler chickens in a salt-induced experimental model. The presence of the enzymes in the lung, heart, liver, kidney and brain was evaluated semi-quantitatively with the streptavidin-biotin-peroxidase (Strep-ABC) method using commercially available primary monoclonal antibodies. Immunostaining of MMP-2 and MMP-9 was more intense and extensive in ascitic broilers than in the controls, although a decrease was seen with increasing age both in normal and ascitic chickens. The presence of MMP-9 enzyme was negatively correlated with the presence of TIMP-1 enzyme. It is suggested that MMP-2 and MMP-9 enzymes might play a role in the permeability increase of vessel walls by the destruction of the basement membranes in the salt-induced experimental ascites syndrome in broiler chickens.
Ozyigit
M O
MO
Department of Pathology, Faculty of Veterinary Medicine, University of Uludag, 16059, Gorukle, Bursa, Turkey.
Kahraman
M M
MM
Sonmez
G
G
D000818:Animals; D001201:Ascites / Q000139:chemically induced / Q000378:metabolism / Q000662:veterinary; D001921:Brain / Q000201:enzymology; D002645:Chickens / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000201:enzymology; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000302:isolation & purification* / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000302:isolation & purification* / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology; D011201:Poultry Diseases / Q000139:chemically induced / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D019714:Tissue Inhibitor of Metalloproteinases / Q000302:isolation & purification* / Q000378:metabolism*
D019714:Tissue Inhibitor of Metalloproteinases; D012965:Sodium Chloride; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9