Radioprotective properties of apple polyphenols: an in vitro study.
Present study was undertaken to evaluate the radioprotective ability of total polyphenols extracted from edible portion (epicarp and mesocarp) of apple. Prior administration of apple polyphenols to murine thymocytes significantly countered radiation induced DNA damage (evaluated by alkaline halo assay) and cell death (trypan blue exclusion method) in a dose dependent manner maximally at a concentration of 2 and 0.2 mg/ml respectively. Apple polyphenols in a dose dependent fashion inhibited both radiation or Fenton reaction mediated 2-deoxyribose (2-DR) degradation indicating its ability to scavenge hydroxyl radicals and this activity was found to be unaltered in presence of simulated gastric juice. Similarly apple polyphenols in a dose dependent fashion scavenged DPPH radicals (maximum 69% at 1 mg/ml), superoxide anions (maximum 88% at 2 mg/ml), reduced Fe(3 +) to Fe(2 +) (maximum at 1 mg/ml) and inhibited Fenton reaction mediated lipid peroxidation (maximum 66% at 1.5 mg/ml) further establishing its antioxidative properties. Studies carried out with plasmid DNA revealed the ability of apple polyphenols to inhibit radiation induced single as well as double strand breaks. The results clearly indicate that apple polyphenols have significant potential to protect cellular system from radiation induced damage and ability to scavenge free radicals might be playing an important role in its radioprotective manifestation.
Department of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Brig SK Mazumdar Marg, Delhi, 110054, India.
Pankaj
P
Chaudhary
Sandeep Kumar
SK
Shukla
I Prem
IP
Kumar
I
I
Namita
Farhat
F
Afrin
Rakesh Kumar
RK
Sharma
D000818:Animals; D001713:Biphenyl Compounds / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D020552:Comet Assay; D003855:Deoxyribose / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D006834:Hydrazines / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D027845:Malus / Q000737:chemistry*; D051379:Mice; D010636:Phenols / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D010851:Picrates; D059808:Polyphenols; D011837:Radiation-Protective Agents / Q000378:metabolism / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism
D001713:Biphenyl Compounds; D005419:Flavonoids; D006834:Hydrazines; D010636:Phenols; D010851:Picrates; D059808:Polyphenols; D011837:Radiation-Protective Agents; D013481:Superoxides; D017665:Hydroxyl Radical; D003855:Deoxyribose; C004931:1,1-diphenyl-2-picrylhydrazyl