The role of preservation solution on acid-base regulation during machine perfusion of kidneys.
To meet the current clinical organ demand, efficient preservation methods and solutions are needed to increase the number of viable kidneys for transplantation. In the present study, the influence of perfusion solution buffering strength on renal pH dynamics and regulation mechanisms during kidney ex vivo preservation was determined. Porcine kidneys were hypothermically machine perfused for 72 h with either Unisol-UHK or Belzer-Machine Perfusion solution, Belzer-MP solution. Renal perfusate samples were periodically collected and biochemically analyzed. The UHK solution, a Hepes-based solution (35 mM), provided a more efficient control of renal pH that, in turn, resulted in minor changes in the perfusate pH relative to baseline, in response to tissue CO2 and HCO3- production. In the perfusate of Belzer-MP kidney group a wider range of pH values were recorded and a pronounced pH reduction was seen in response to significant rises in pCO2 and HCO3- concentrations. The Belzer-MP solution, containing phosphate (25 mM) as its main buffer, and only 10 mM Hepes, had a greater buffering requirement to attenuate larger pH changes.
Baicu
Simona C
SC
Organ Recovery Systems, Inc., Charleston, SC 29403, USA. sbaicu@organ-recovery.com
Taylor
Michael J
MJ
Brockbank
Kelvin G M
KG
D000136:Acid-Base Equilibrium / Q000187:drug effects*; D000241:Adenosine / Q000737:chemistry / Q000494:pharmacology; D000493:Allopurinol / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002021:Buffers; D005978:Glutathione / Q000737:chemistry / Q000494:pharmacology; D007328:Insulin / Q000737:chemistry / Q000494:pharmacology; D016030:Kidney Transplantation; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions / Q000737:chemistry / Q000494:pharmacology*; D010477:Perfusion; D011887:Raffinose / Q000737:chemistry / Q000494:pharmacology; D013552:Swine; D013696:Temperature
D002021:Buffers; D007328:Insulin; D019188:Organ Preservation Solutions; C513328:UHK solution; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose