Improved pharmacological properties for superoxide dismutase modified with mannan.
Mannan from Sacharomyces cerevisiae was activated by oxidation with NaIO(4) (sodium m-periodate) and further linked to SOD (superoxide dismutase) via reductive alkylation with NaBH(4) (sodium borohydride). The glycosidated enzyme contained an average of 1.2 mol of polysaccharide per mol of protein and retained 52% of its initial activity. The modified enzyme was 560-fold more resistant to inactivation with H(2)O(2) and acquired a lectin-recognition capacity in respect of concanavalin A. The anti-inflammatory activity of SOD was increased 2-fold and its plasma half-life time was prolonged from 4.8 min to 1.7 h after glycosylation with the polymer.
Valdivia
Aymara
A
Center for Enzyme Technology, University of Matanzas, Autopista a Varadero Km 3 1/2, Matanzas, C.P. 44740, Cuba.
Pérez
Yunel
Y
Dominguez
Amalia
A
Caballero
Julio
J
Hernández
Yunior
Y
Villalonga
Reynaldo
R
D000478:Alkylation; D000818:Animals; D001894:Borohydrides / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002351:Carrageenan; D004789:Enzyme Activation / Q000187:drug effects; D006031:Glycosylation; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D008351:Mannans / Q000737:chemistry*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D013329:Structure-Activity Relationship; D013482:Superoxide Dismutase / Q000097:blood / Q000737:chemistry* / Q000627:therapeutic use*; D013997:Time Factors
D001894:Borohydrides; D008351:Mannans; D010504:Periodic Acid; C025364:sodium borohydride; D002351:Carrageenan; C009288:metaperiodate; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase