First Spanish case of thalassemia major due to a compound heterozygosity for the IVS-II-848 (C --> A) and codon 39 (C --> T) mutations of the beta-globin gene.
This report describes the first case in Spain of a severe form of beta-thalassemia (thal) due to a compound heterozygosity for the IVS-II-848 (C --> A) and the nonsense codon 39 (C --> T) mutations. Five members of a family from Cadiz (southern Spain) were studied. The proband was an 8-year-old girl diagnosed as anemic at the age of 13 months. Her father had the codon 39 (C --> T) mutation and her mother the C --> A change at nucleotide (nt) 848 of IVS-II. Haplotype analysis showed that the proband was a compound heterozygote for haplotypes I [+ --> + +] and VII [+ --> +]. This is the first description in Spain of the IVS-II-848 (C --> A) mutation. It appears, from restriction fragment length polymorphism (RFLP) analysis, that this mutation has a different origin in the various populations, where it was found. This observation shows that in this case the association of a beta(0)- and a beta(+)-thal mutation does not lead to a thalassemia intermedia but to a severe thalassemia with very low hemoglobin (Hb) levels. From a therapeutic point of view, early introduction of a transfusion regimen may improve the clinical picture of these children, allowing for better development and growth.
Servicio de Hematología y Hemoterapia, Hospital Clínico San Carlos de Madrid, Madrid, España.
Paloma
P
Ropero
Ana
A
Villegas
Juan
J
Muñoz
Olga
O
Briceño
Asunción
A
Mora
María
M
Salvador
Marta
M
Polo
Fernando A
FA
González
D000293:Adolescent; D000328:Adult; D000483:Alleles; D002648:Child; D018389:Codon, Nonsense; D005192:Family Health; D005260:Female; D005914:Globins / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D008297:Male; D020125:Mutation, Missense; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D035781:Siblings; D013030:Spain / Q000453:epidemiology; D017086:beta-Thalassemia / Q000175:diagnosis / Q000235:genetics*
D018389:Codon, Nonsense; D005914:Globins