Poly(dimethyl siloxane)-based protein chip for simultaneous detection of multiple samples: use of glycidyl methacrylate photopolymer for site-specific protein immobilization.
This paper describes fabrication of a poly(dimethyl siloxane) (PDMS)-based chip to analyze multiple protein interactions utilizing glycidyl methacrylate (GMA) photopolymer for a site-specific immobilization of capture proteins in a closed system. First, using one direction channels of a PDMS mold having cross-channels, GMA micropads were prepared by photopolymerizing GMA solution by 365 nm light irradiation at predetermined positions. After the first mold was replaced with a second mold having higher height or directly without mold changing, capture proteins were allowed to be covalently immobilized onto the surface of the epoxide-activated GMA pads. Following immobilization, poly(ethylene glycol) diacrylate (PEG-DA) precursor was photopolymerized at specific regions to generate plugs for prevention of mixing between different sample injection channels, diminishing the need of a mold changing for sample injections. Final chip was assembled by connecting separated sample injection channels using a connector mold. The viability of this strategy was successfully demonstrated by simultaneous detection of two different antigen-antibody interactions.
Park
Kyoung Hwan
KH
Department of Chemical and Biomolecular Engineering, Korea Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon, Republic of Korea.
Park
Hyun Gyu
HG
Kim
Joon-Ho
JH
Seong
Ki Hun
KH
D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D045424:Complex Mixtures / Q000032:analysis; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry* / Q000528:radiation effects; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008027:Light; D008689:Methacrylates / Q000737:chemistry* / Q000528:radiation effects; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D010777:Photochemistry / Q000379:methods; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011485:Protein Binding; D012828:Silicones / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D013499:Surface Properties
D001672:Biocompatible Materials; D045424:Complex Mixtures; D004129:Dimethylpolysiloxanes; D004852:Epoxy Compounds; D008689:Methacrylates; D012828:Silicones; C013830:baysilon; C007870:glycidyl methacrylate