Selection of streptavidin binders from a DNA-encoded chemical library.
DNA-encoded libraries of small organic molecules facilitate the construction of large, encoded self-assembling chemical libraries for the identification of high-affinity binders to protein targets. We have constructed a library of 477 chemical compounds, coupled to 48mer-oligonucleotides, each containing a unique six-base sequence serving as "bar-code" for the identification of the chemical moiety. The functionality of the library was confirmed by selection and amplification of both high- and low-affinity binding molecules specific to streptavidin.
Dumelin
Christoph E
CE
Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland.
Scheuermann
Jörg
J
Melkko
Samu
S
Neri
Dario
D
D000818:Animals; D004247:DNA / Q000737:chemistry*; D015723:Gene Library; D006801:Humans; D015394:Molecular Structure; D011485:Protein Binding; D019809:Streptavidin / Q000737:chemistry* / Q000378:metabolism
D004247:DNA; D019809:Streptavidin