Group-type analysis of oxygenated compounds with a silica gel porous layer open tubular column and comprehensive two-dimensional supercritical fluid and gas chromatography.
A porous layer open tubular (PLOT) silica gel column was used together with subcritical CO2 as the mobile phase to effect the group separation of polar oxygenated compounds. Aliphatic and aromatic compounds were shown to elute together. This group was followed by ethers and aldehydes, which were separated from compounds containing an alcohol functional group. Compounds with a carboxylic acid moiety could also be eluted from the silica gel. The group separation obtained when a silica gel PLOT column is used together with subcritical CO2 was also demonstrated to be valuable as the first dimension of a comprehensive two-dimensional SFCxGC analysis where the GC analysis in the second dimension is performed with a fast and independently heated temperature programmed gas chromatograph. With this combination of SFC and GC, many of the oxygenated compounds, routinely found in petroleum samples, could successfully be separated and identified.
Chemistry Department, University of Pretoria, Pretoria, South Africa.
Andre
A
Venter
Peter R
PR
Makgwane
Egmont R
ER
Rohwer
D000447:Aldehydes / Q000032:analysis*; D002264:Carboxylic Acids / Q000032:analysis*; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation / Q000379:methods*; D005782:Gels / Q000737:chemistry; D006838:Hydrocarbons / Q000032:analysis; D007659:Ketones / Q000032:analysis*; D016062:Porosity; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*
D000447:Aldehydes; D002264:Carboxylic Acids; D005782:Gels; D006838:Hydrocarbons; D007659:Ketones; D012822:Silicon Dioxide