BacA-mediated bleomycin sensitivity in Sinorhizobium meliloti is independent of the unusual lipid A modification.
Sinorhizobium meliloti bacA mutants are symbiotically defective, deoxycholate sensitive, and bleomycin resistant. We show that the bleomycin resistance phenotype is independent of the lipid A alteration and that the changes giving rise to both phenotypes are likely to be involved in the inability of bacA mutants to persist within their hosts.
Ferguson
Gail P
GP
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. G.Ferguson@ed.ac.uk
Jansen
An
A
Marlow
Victoria L
VL
Walker
Graham C
GC
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D001761:Bleomycin / Q000494:pharmacology*; D003840:Deoxycholic Acid / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D017353:Gene Deletion; D005798:Genes, Bacterial; D008050:Lipid A / Q000378:metabolism*; D016962:Sinorhizobium meliloti / Q000187:drug effects* / Q000254:growth & development; D013559:Symbiosis / Q000235:genetics / Q000502:physiology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008050:Lipid A; D003840:Deoxycholic Acid; D001761:Bleomycin