Influence of surface pretreatment of titanium- and cobalt-based biomaterials on covalent immobilization of fibrillar collagen.
Collagen type-I is a major component of the extracellular matrix of most tissues and it is increasingly utilized for surface engineering of biomaterials to accelerate receptor-mediated cell adhesion. In the present study, coatings with layers of fibrillar type-I collagen were prepared on titanium, titanium alloy, and cobalt alloy to improve initial osteoblast adhesion and implant-tissue integration. To suppress the quick in vivo degradation rate of collagen the deposited layers were covalently immobilized at the metal surfaces as well as chemically cross-linked. The application of different oxidation techniques to the metallic substrates resulted in surfaces with varying hydroxyl group contents, which directly influenced the amount of immobilized silane coupling agents. It was found that a high density of surface-bound coupling agents increased the stability of the covalently linked collagen layers. After coating of metallic biomaterials with a cross-linked collagen layer, an improved cellular response of human osteoblast-like cells (MG-63) in vitro could be recognized.
Institute of Physical and Theoretical Chemistry, University of Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany. rainer.meuller@chemie.uni-regenburg.de
Rainer
R
Müller
Jochen
J
Abke
Edith
E
Schnell
Dieter
D
Scharnweber
Richard
R
Kujat
Carsten
C
Englert
Darius
D
Taheri
Michael
M
Nerlich
Peter
P
Angele
D001672:Biocompatible Materials / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D024022:Fibrillar Collagens / Q000737:chemistry*; D006801:Humans; D008670:Metals / Q000737:chemistry; D010006:Osteoblasts / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D012821:Silanes / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D024022:Fibrillar Collagens; D008670:Metals; D012821:Silanes; D003035:Cobalt; D003094:Collagen; D014025:Titanium; D010100:Oxygen