Effect of gonadotrophin stimulation on mouse oocyte quality and subsequent embryonic development in vitro.
In-vivo-matured oocytes were collected from naturally ovulated and superovulated [pregnant mare's serum gonadotrophin (PMSG) + human chorionic gonadotrophin (HCG)] mice. Immature oocytes were retrieved from naturally cycling mice and from mice primed with PMSG. The percentages of cleavage and blastocyst formation were significantly different (P < 0.05) between in-vivo- and in-vitro-matured oocytes. Blastocyst formation rate was significantly higher (P < 0.05) in immature oocytes derived from PMSG-primed mice, and the percentages of oocytes with comet tails, and their length, were significantly higher and longer respectively in in-vitro-matured oocytes. Total cell numbers of blastocysts were also significantly different (P < 0.05) between in-vivo- and in-vitro-matured oocytes, but there were also no differences in ratio of trophectoderm (TE)/inner cell mass (ICM). In conclusion, in-vivo-matured mouse oocytes were more competent than those matured in-vitro, perhaps due to a lesser degree of DNA damage. Embryonic development capacity of in-vivo-matured oocytes is not promoted by ovarian stimulation. Gonadotrophin priming prior to immature mouse oocyte retrieval is beneficial to subsequent embryonic development.
Wang
Yue
Y
Division of Reproductive Biology and Experimental Medicine, Department of Obstetrics and Gynecology, McGill University, Women's Pavilion F3, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Canada H3A 1A1.
Ock
Sun-A
SA
Chian
Ri-Cheng
RC
D000818:Animals; D001562:Benzimidazoles; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D020552:Comet Assay; D053938:DNA Fragmentation / Q000187:drug effects / Q000502:physiology; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000592:standards; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011419:Propidium; D013480:Superovulation / Q000187:drug effects / Q000502:physiology
D001562:Benzimidazoles; D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D011419:Propidium; C017807:bisbenzimide ethoxide trihydrochloride