Time course and differential responses of the major heat shock protein families in human skeletal muscle following acute nondamaging treadmill exercise.
The exercise-induced expression of heat shock proteins (HSPs) in rodent models is relatively well defined. In contrast, comparable data from human studies are limited and the exercise-induced stress response of human skeletal muscle is far from understood. This study has characterized the time course and magnitude of the HSP response in the skeletal muscles of a healthy active, but untrained, young male population following a running exercise protocol. Eight subjects performed 45 min of treadmill running at a speed corresponding to their lactate threshold (11.7 +/- 0.5 km/h; 69.8 +/- 4.8% maximum O2 uptake). Muscle biopsies were obtained from the vastus lateralis muscle immediately before and at 24 h, 48 h, 72 h, and 7 days postexercise. Exercise induced a significant (P < 0.05) but variable increase in HSP70, heat shock cognate (HSC) 70, and HSP60 expression with peak increases (typically occurring at 48 h postexercise) to 210, 170, and 139% of preexercise levels, respectively. In contrast, exercise did not induce a significant increase in either HSP27, alphaB-crystallin, SOD 2 (MnSOD) protein content, or the activity of SOD and catalase. When examining baseline protein levels, HSC70, HSP27, and alphaB-crystallin appeared consistently expressed between subjects, whereas HSP70 and MnSOD displayed marked individual variation of up to 3- and 1.5-fold, respectively. These data are the first to define the time course and extent of HSP production in human skeletal muscle following a moderately demanding and nondamaging running exercise protocol. Data demonstrate a differential effect of aerobic exercise on specific HSPs.
Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, 15-21 Webster St., Liverpool L3 2ET, UK. SPSJMORT@livjm.ac.uk
James P
JP
Morton
Don P M
DP
MacLaren
Nigel T
NT
Cable
Thomas
T
Bongers
Richard D
RD
Griffiths
Iain T
IT
Campbell
Louise
L
Evans
Anna
A
Kayani
Anne
A
McArdle
Barry
B
Drust
D000328:Adult; D001706:Biopsy; D002374:Catalase / Q000378:metabolism; D018834:Chaperonin 60 / Q000378:metabolism; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D005786:Gene Expression Regulation; D050883:HSC70 Heat-Shock Proteins / Q000378:metabolism; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D018832:Molecular Chaperones; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D013997:Time Factors; D038203:alpha-Crystallin B Chain / Q000378:metabolism
D018834:Chaperonin 60; D050883:HSC70 Heat-Shock Proteins; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; C000606290:SOD1 protein, human; D038203:alpha-Crystallin B Chain; D002374:Catalase; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1