Culture conditions improving the production of jadomycin B.
The jadomycins are a unique family of benzoxazolophenanthridine antibiotics produced by Streptomyces venezuelae ISP5230 following heat or ethanol shock or phage infection. We have modified the culture conditions by altering the carbon source, buffer, inoculum size, and timing of ethanol shock, thereby reducing growing times and improving jadomycin B production. Our optimized conditions use glucose as the carbon source, MOPS as buffer, low concentrations of phosphate, a defined inoculum concentration and an immediate ethanol shock to induce jadomycin B production; results that contrast previous studies. The altered media will facilitate the isolation of related jadomycin B congeners.
Jakeman
David L
DL
College of Pharmacy, Dalhousie University, Halifax, NS, Canada. david.jakeman@dal.ca
Graham
Cathy L
CL
Young
Wendy
W
Vining
Leo C
LC
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism; D001431:Bacteriological Techniques / Q000379:methods*; D001709:Biotechnology / Q000379:methods*; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D007546:Isoquinolines / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D009025:Morpholines / Q000737:chemistry / Q000378:metabolism; D009285:Naphthoquinones / Q000737:chemistry / Q000378:metabolism; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D011508:Chondroitin Sulfate Proteoglycans; D003470:Culture Media; D007546:Isoquinolines; D009025:Morpholines; D009285:Naphthoquinones; C506597:jadomycin A; C081750:jadomycin B; C008550:morpholinopropane sulfonic acid; D000431:Ethanol; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D005947:Glucose