Remodeling of myocardial sleeve and gap junctions in canine superior vena cava after rapid pacing.
OBJECTIVE
We studied the response of the superior vena cava (SVC) myocardial sleeve to atrial fibrillation (AF).


RESULTS
We examined adult male dogs without pacing (N=6) and after rapid atrial pacing (600 bpm) for 2 weeks (P2w; N=5) and 6-8 weeks (P6-8w; N=5). After pacing, the sleeve was increased in thickness (non-paced vs. either paced group, both P<0.05). This was associated with an increase in proliferative activity, which was higher in the P2w than the P6-8w animals (P<0.05). In addition, collagen content increased, and the component cardiomyocytes become more unevenly oriented and shorter and narrower in shape (non-paced vs. either paced group, both P<0.05). Pacing had different effects on connexin40 (Cx40) and Cx43 gap junctions. There was a 98% increase in Cx43 signal in P2w, and a 74% increase in P6-8w animals (non-paced vs. each paced group, both P<0.05). In contrast, Cx40 signal decreased 47% in P2w but increased 44% in P6-8w animals (non-paced vs. each paced group, both P<0.05).


CONCLUSIONS
Rapid atrial pacing results in a specific pattern of remodeling of the canine SVC sleeve, including changes in size and shape, spatial orientation, and gap junction expression profile of the component cardiomyocytes. These changes may co-operatively affect the electrical properties and contribute to the formation and maintenance of the arrhythmogenic substrate of AF.
Yeh
Hung-I
HI
Department of Internal Medicine and Medical Research Mackay Memorial Hospital, Mackay Medicine Nursing and Management College, Taipei Medical University, 92, Sec 2, Chung San North Road, 10449, Taipei, Taiwan.
Lai
Yu-Jun
YJ
Lee
Shih-Huang
SH
Chen
Sin-Tai
ST
Ko
Yu-Shien
YS
Chen
Shih-Ann
SA
Severs
Nicholas J
NJ
Tsai
Cheng-Ho
CH
D000818:Animals; D001281:Atrial Fibrillation / Q000473:pathology*; D002304:Cardiac Pacing, Artificial; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism; D017630:Connexins / Q000378:metabolism; D004285:Dogs; D017629:Gap Junctions / Q000473:pathology*; D009206:Myocardium / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000473:pathology*; D014683:Vena Cava, Superior / Q000473:pathology*
D017630:Connexins; D003094:Collagen