[Research on correlations of quality and growing environment of Smilax China].
OBJECTIVE
To establish a method to examine the contents of diosgenin of Smilax china L. in different fields ,and to evaluate the influence of the growing environment on its quality.


METHODS
Kromasie C18 column was used. The mobile phase was CH3OH-H2O (94:6), flow rate: 1.0 ml/min,the detective wavelength was 210 nm.


RESULTS
Under the chromatographic condition the diosgenin can be completely separated,in the range of 5 microg-15 microg diosgenin had fine linear responses The mean recovery was 98.47%, and the RSD was 1.01%.


CONCLUSIONS
The method was sensitive, simple and accurate. There were some correlations on the quality of Smilax china L. and growing environment.
Hubei College of Traditional Chinese Medicine, Wuhan.
Shuzhen
S
Xu
Guangzhong
G
Wang
Guoping
G
Gan
Qiuyi
Q
Li
Yanwen
Y
Liu
D000531:Altitude; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004144:Diosgenin / Q000032:analysis*; D017753:Ecosystem; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D027343:Rhizome / Q000737:chemistry; D032281:Smilax / Q000737:chemistry*; D012987:Soil
D012987:Soil; D004144:Diosgenin