Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens.
The Ti plasmid sequences (T-DNA) from the octopine-producing crown gall tumor A6S/2 were isolated by molecular cloning, using the bacteriophage lambda vector Charon 4A. Analysis of the cloned DNA segments indicates that the Ti plasmid sequences are covalently joined to plant nuclear DNA. These data demonstrate that genetic recombination between a eukaryote and a prokaryote can occur as a natural phenomenon.
Department of Microbiology and Immunology, University of Washington, Seattle, Washington 98195.
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Thomashow
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Nutter
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Postle
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Chilton
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Blattner
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Powell
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Gordon
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Nester