New real-time PCR assay using locked nucleic acid probes to assess prevalence of ParC mutations in fluoroquinolone-susceptible Streptococcus pneumoniae isolates from France.
A real-time PCR assay with locked nucleic acid probes was developed to screen mutations at codons 79 and 83 of the Streptococcus pneumoniae parC gene. Only silent mutations were detected among 236 French invasive fluoroquinolone-susceptible strains. This test could be useful for some high-risk patients or in national surveys.
Laboratoire de Biologie, Centre Hospitalier de Dourdan, 2 Rue du Potelet, 91415 Dourdan, France. jean-winoc.decousser@wanadoo.fr
Jean-Winoc
JW
Decousser
Imen
I
Methlouthi
Patrick
P
Pina
Anne
A
Collignon
Pierre
P
Allouch
D000890:Anti-Infective Agents / Q000494:pharmacology*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D024841:Fluoroquinolones / Q000494:pharmacology*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000235:genetics
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D027101:DNA Topoisomerase IV