Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity.
OBJECTIVE
To determine whether hypothermia of 8 degrees C can protect cultured human retinal pigment epithelial (ARPE-19) cells and rat retinal ganglion cells (RGC-5) against trypan blue (TB) toxicity.


METHODS
Laboratory investigation.


METHODS
ARPE-19 cells and RGC-5 were exposed to balanced salt solution as controls, and 0.05% and 0.5% TB at 37 degrees C, and at 8 degrees C for one minute. The percentage of surviving cells was determined by the resazurin test.


RESULTS
TB induced a statistically significant decrease in the percentage of ARPE-19 cells surviving at 0.5% TB at 37 degrees C (P < .01). Conversely, TB induced a statistically significant decrease in the percentage of RGC-5 surviving at all conditions except for 0.05% TB at 8 degrees C (0.05% 37 degrees C; P < .05, 0.5% 37 degrees C and 8 degrees C; P < .01).


CONCLUSIONS
These results indicate that reducing the temperature to 8 degrees C has a protective effect against the TB toxicity for ARPE-19 cells and RGC-5 in culture.
Kunikata
Hiroshi
H
Department of Ophthalmology and Visual Science, Tohoku University Graduate School of Medicine, Japan. kunikata@oph.med.tohoku.ac.jp
Abe
Toshiaki
T
Murata
Hiromi
H
Sagara
Yoshiko
Y
Wakusawa
Ryosuke
R
Sato
Hajime
H
Yoshida
Madoka
M
Fuse
Nobuo
N
Tamai
Makoto
M
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D004396:Coloring Agents / Q000633:toxicity*; D019610:Cytoprotection; D064420:Drug-Related Side Effects and Adverse Reactions / Q000517:prevention & control*; D006801:Humans; D006979:Hyperthermia, Induced; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000187:drug effects; D014343:Trypan Blue / Q000633:toxicity*
D004396:Coloring Agents; D014343:Trypan Blue