[Primary culture of human omental preadipocytes].
OBJECTIVE
To establish a primary culture method of human omental preadipocytes.


METHODS
Using enzyme-digesting method, fibroblast-like cells from the human omental adipose tissues were cultured, and then differentiated by conditional medium, and identified by oil red O staining.


RESULTS
The cultured cells were highly homogeneous, and highly proliferative in 4-5th generation. During the process of induction by conditional medium, the cells became round-like and larger, and more adipose droplets were aggregated. By oil red O staining, we confirmed the differentiated cells were mature adipocytes.


CONCLUSIONS
In human omental adipose tissues, there are some preadipocytes, which can differentiate into mature adipocytes with appropriate stimulus.
Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China. szf1976@hotmail.com
Zhi-feng
ZF
Sheng
Ping-fang
PF
Wang
Wei
W
Wang
Jing
J
He
Wen-jing
WJ
Li
D017667:Adipocytes / Q000166:cytology*; D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D006801:Humans; D008297:Male; D009852:Omentum / Q000166:cytology*
D017077:Culture Media, Conditioned