Simple and rapid high-performance liquid chromatographic method for determination of celecoxib in plasma using UV detection: application in pharmacokinetic studies.
A rapid, sensitive and reproducible HPLC method was developed and validated for the analysis of celecoxib in human plasma. The analysis was carried out on a monolithic silica column using UV detection at 254 nm. The assay enables the measurement of celecoxib for therapeutic drug monitoring with a minimum quantification limit of 10 ng ml(-1). The method involves simple, one-step extraction procedure, and analytical recovery was 100.5 +/- 1.3%. The calibration curve was linear over the concentration range of 10-800 ng ml(-1). The coefficients of variation for inter-day and intra-day assay were found to be less than 8%. We also demonstrate the applicability of this method for pharmacokinetic studies in humans.
Department of Pharmaceutical Chemistry, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, No. 10, Shams Alley, Across from Tavanir St., Vali-E-Asr Ave., Tehran, Iran. azarghi@yahoo.com
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Zarghi
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Shafaati
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Foroutan
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Khoddam
D000068579:Celecoxib; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D011720:Pyrazoles / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013449:Sulfonamides / Q000097:blood* / Q000493:pharmacokinetics; D014466:Ultraviolet Rays
D011720:Pyrazoles; D013449:Sulfonamides; D000068579:Celecoxib