[A novel model for isolation of Walker's tumoral cells using the Ficoll-Hypaque gradient].
OBJECTIVE
The purpose was to evaluate a novel technique for isolation of Walker's tumoral cells using a Ficoll-Hypaque gradient and its further influence on tumor development.


METHODS
Twenty male Wistar rats have been divided in 2 groups: G1= without ficoll, G2= with ficoll. Tumor was excised, homogenized and suspended in lactate ringer. A sample of the cell suspension was adjusted at a concentration of 1x10(6) cells/ml (G1). A second sample was centrifuged on a Ficoll-Hypaque gradient and the cell concentration was then adjusted (G2). Tumor was implanted by subcutaneous injection of 1.0 ml in the right armpit of rats. Tumor volume (TV) and tumor weight (TW) were compared in two groups.


RESULTS
There were no differences between the two groups in TV (G1=17.9+/-3.8 cm3 vs. G2=17.2+/-4.4 cm3; p=0.190) and TW (G1=7.0+/-1.8 g vs. G2=7.3+/-2.8 g; p=0.569). The histological analysis showed similar patterns of infiltration by small-undifferentiated cells and necrosis in both groups. However, a mild to moderate granulocytic exudate was more frequent in the animals whose tumors derived from Ficoll-isolated cells. Hemorrhage from slight to moderate was only observed in this group.


CONCLUSIONS
A Ficoll-Hypaque gradient can provide more adequate isolation of Walker's tumor and the cell suspension obtained by this technique has lower contamination by other cell types.
Silva
Sônia Leite
SL
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza-CE, Brazil. sonials@unifor.br
Silva
Silvia Fernandes Ribeiro
SF
Farias
Italo Nascimento
IN
Mota
Rosa Salani
RS
Moraes
Maria Elisabete
ME
Campos
Henry de Holanda
Hde H
Ferreira
Francisco Valdeci
FV
Moraes Filho
Manoel Odorico
MO
D000818:Animals; D002279:Carcinoma 256, Walker / Q000737:chemistry / Q000473:pathology*; D002469:Cell Separation / Q000379:methods* / Q000592:standards; D002499:Centrifugation, Density Gradient / Q000592:standards*; D004195:Disease Models, Animal; D005362:Ficoll / Q000737:chemistry*; D008297:Male; D015999:Multivariate Analysis; D051381:Rats; D017208:Rats, Wistar
D005362:Ficoll