Kocuria aegyptia sp. nov., a novel actinobacterium isolated from a saline, alkaline desert soil in Egypt.
A coccoid, non-motile actinobacterium, designated strain YIM 70003T, was isolated from a saline, alkaline, desert-soil sample from Egypt. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism formed a distinct phyletic line within the genus Kocuria and was most closely related to Kocuria polaris DSM 14382T (98.6 % sequence similarity) and Kocuria rosea DSM 20447T (98.2 %). Chemotaxonomic data, including the Lys-Ala3 peptidoglycan type, the presence of phosphatidylglycerol and diphosphatidylglycerol as the predominant phospholipids, the presence of MK-8(H2) and MK-9(H2) as the major menaquinones, the predominance of fatty acids ai-C(15 : 0) and i-C(15 : 0) and the DNA G+C content, also supported the affiliation of the isolate to the genus Kocuria. The low DNA-DNA relatedness with K. polaris DSM 14382T (56.6 %) and K. rosea DSM 20447T (15.5 %) in combination with phenotypic data show that strain YIM 70003T should be classified as a novel species of the genus Kocuria. The name Kocuria aegyptia sp. nov. is proposed, with strain YIM 70003T (=CCTCC AA203006T = CIP 107966T = KCTC 19010T = DSM 17006T) as the type strain.
Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People's Republic of China.
Wen-Jun
WJ
Li
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, People's Republic of China.|Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People's Republic of China.
Yu-Qin
YQ
Zhang
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Peter
P
Schumann
Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People's Republic of China.
Hua-Hong
HH
Chen
Botany Department, Faculty of Science, Cairo University, Beni-Suef, Egypt.
Wael N
WN
Hozzein
Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People's Republic of China.
Xin-Peng
XP
Tian
Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People's Republic of China.
Li-Hua
LH
Xu
Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People's Republic of China.
Cheng-Lin
CL
Jiang
D039903:Actinobacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D003889:Desert Climate; D004534:Egypt; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012965:Sodium Chloride; D012988:Soil Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride