[An experimental study on the culture of human oral mucosa epithelium using autologous serum].
OBJECTIVE
To investigate the biological properties of cultured human oral mucosa epithelium using autologous serum in order to provide a new material for tissue engineering urethra.


METHODS
The cultured oral mucosa epithelium was respectively transplanted beneath the skin, above the deep fascia and in the wound of the athymic mice. The specimens were taken at 2, 3, 4, and 6 weeks posttransplantation, and processed for (1) immunofluorescence anti-HLA staining to determine graft acceptance, and (2) anti-human IV collagen and antihuman laminin immunohistochemical staining procedures to indicate the basement membrane formation.


RESULTS
All the grafts survived and grew very well. The grafts were positive to anti-HLA. Collagen type IV and laminin were detected at the dermo-epidermal junction in all groups from day 14, and increasing in density up to day 21.


CONCLUSIONS
he cultured human oral mucosa epithelium by autologous serum could develop an excellent functional epithelium tissue, which would be used to reconstruct urethra and repair wound.
Plastic Surgery Hospital of CAMS, Beijing 100041, China.
Li-qiang
LQ
Liu
Sen-kai
SK
Li
Yong-qian
YQ
Wang
Jin-cai
JC
Fan
Yang-qun
YQ
Li
Yuan-bo
YB
Liu
Jia-jie
JJ
Xu
Chuan-de
CD
Zhou
D000818:Animals; D002478:Cells, Cultured; D004848:Epithelium / Q000637:transplantation*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009061:Mouth Mucosa / Q000166:cytology*; D044967:Serum; D016038:Skin Transplantation; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D016378:Tissue Transplantation / Q000379:methods; D014183:Transplantation, Heterologous