[Genetic diversity and phylogeny of soybean bradyrhizobia isolated from south and north region of China].
Studies on genetic diversity and phylogeny of soybean bradyrhizobia isolated from south and north region of China were investigated through 16S rRNA gene PCR RFLP, 16S rRNA gene sequencing and 16S-23S rRNA IGS PCR RFLP assays. Results of 16S rRNA gene PCR RFLP and 16S rRNA gene sequencing analysis reveal that strains tested are ascribed into Bradyrhizobium japonicum and B. elkanii species. B. japonicum is the dominant species and accounts for 91% of strains tested, but B. elkanii just up to 9%, which shows poor genetic diversity. Results of 16S-23S rRNA IGS PCR RFLP assays reveal that strains belonged to B. japonicum can be divided into Group I and Group HIIat the 69 similarity. Group I consists of strains from northern China, and Group 1I consists of strains from southern China, which demonstrates geographical effect on genetic diversity of bradyrhzobia. Results of 16S-23S rRNA IGS PCR RFLP further reflects that strains of Group I and Group II are phylogenetically different with type or representative strains USDA6, USDA110 and USDA122 of B. japonicum.
Zhang
Wei-tao
WT
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. zhweitao888@webmail.hzau.edu.cn
Yang
Jiang-ke
JK
Yuan
Tian-ying
TY
Zhou
Jun-chu
JC
D020369:Bradyrhizobium / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D002681:China; D014644:Genetic Variation; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012988:Soil Microbiology
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S