The influence of various genotypes on the metabolic activity of NAT2 in a Chinese population.
OBJECTIVE
To determine the phenotype and genotype of NAT2 in a Chinese population and study the influence of various NAT2 genotypes on the NAT2 activity.


METHODS
A reverse dot blot method was used to detect the genotype of NAT2 in 120 healthy Chinese subjects. All subjects were given a single dose of 500 mg sulphadimidine (SM(2)). The plasma concentration of SM(2) and acetyl-SM(2) (AcSM(2)) 6 h after administration was determined. Molar metabolic ratio (MR) was calculated by the ratio of AcSM(2) to AcSM(2)+SM(2).


RESULTS
Totals of 53 (44.2%), 47 (39.2%) and 20 (16.7%) subjects were homozygotes for wild type (wt/wt), heterozygotes for mutant (m/wt) and homozygotes for mutant (m/m), respectively. The MR of 120 subjects was 0.714+/-0.237. Twenty subjects (16.7%) were classified as poor metabolizers. All subjects in the m/m group were poor metabolizers. The MRs of the wt/wt, m/wt and m/m groups were 0.886+/-0.060, 0.719+/-0.089 and 0.246+/-0.105 (P<0.001), respectively. There was a significant difference between different NAT2 m/wt genotypes (P<0.001) and m/m genotypes (P<0.001). MR correlated well with NAT2 genotypes (r=0.947).


CONCLUSIONS
Various NAT2 genotypes have a significant impact on the metabolic activity of NAT2 in Chinese people.
Chen
Bing
B
Department of Pharmacy, Ruijin Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200025, People's Republic of China.
Zhang
Wei-Xia
WX
Cai
Wei-Min
WM
D000328:Adult; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D044466:Asian People; D002681:China; D005260:Female; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D010641:Phenotype; D013418:Sulfamethazine / Q000493:pharmacokinetics
D013418:Sulfamethazine; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human