Single-step DNA immobilization on antifouling self-assembled monolayers covalently bound to silicon (111).
Hydrosilylation of alkenes with epoxide-terminated tri(ethylene oxide) moieties on Si-H surfaces yields homogeneous monolayers for the efficient coupling of biomolecules. The wetting properties of the epoxide-functionalized surface allow for the spotting of solutions of biomolecules, making the surface amenable to microarraying. Immobilization of thiolated DNA was achieved in a single step to fabricate biorecognition interfaces showing the hybridization of complementary DNA at low concentrations and negligible binding of noncomplementary DNA.
School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia.
Till
T
Böcking
Kristopher A
KA
Kilian
Katharina
K
Gaus
J Justin
JJ
Gooding
D000475:Alkenes / Q000737:chemistry; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010087:Oxides / Q000737:chemistry; D012666:Semiconductors; D012821:Silanes / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties
D000475:Alkenes; D004852:Epoxy Compounds; D010087:Oxides; D012821:Silanes; D013438:Sulfhydryl Compounds; D006859:Hydrogen; D004247:DNA; D012825:Silicon