Optimization of the MhcOvar-DRB1 gene typing.
The variability of the sheep major histocompatibility complex (MHC) class II DRB1 locus has been analyzed in this work. Exon 2 of Ovar-DRB1 was amplified by polymerase chain reaction (PCR) with the primers designed by Amills et al. for goats. In a total of 187 sheep of Latxa breed, we identified by PCR-single-strand conformation polymorphism (SSCP) 19 alleles, eight of them previously unpublished. Moreover, the observed heterozygosity reached 91-95%. A new allelic type named DRB*14 was defined, which brings to light once more the discussion as to the ancestor of the domestic sheep. Although all the defined alleles can be discriminated by PCR-SSCP, sequence-based typing is proposed as the technique of choice for sheep DRB1 gene typing.
Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, Bilbao, Spain.
I
I
Arrieta-Aguirre
K
K
García-Etxebarria
B M
BM
Jugo
D000483:Alleles; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D005091:Exons; D005787:Gene Frequency; D014644:Genetic Variation; D006579:Heterozygote; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000145:classification / Q000235:genetics* / Q000276:immunology; D009626:Terminology as Topic
D000949:Histocompatibility Antigens Class II