[In vivo functional microspectroscopy of cells and tissues].
The interaction between living matter and optical spectra (Raman, infrared, fluorescence spectroscopy) can be used to characterize simple or complex biomolecular systems. At the cell and tissue level, these microspectroscopic techniques can be used to construct descriptors of molecular or supramolecular (nucleic acids, proteins, lipids, etc.) function in a given biological situation (healthy or pathological tissue, normal or pathological cell, induction of cytotoxicity, apoptosis or differentiation, presence or not of a resistance phenotype, etc.) At the present time vibrational spectroscopy appears to be a particularly powerful technique for characterization of the early phases of pathological processes occurring within given cells or tissues. It could be used to develop tools for early diagnosis and prediction of therapeutic response.
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Unité MEDIAN, CNRS UMR 6142, UFR Pharmacie, IFR 53, 51, rue Cognacq Jay, F5109 6 Reims Cedex. michel.manfait@univ-reims.fr
D002477:Cells / Q000737:chemistry*; D003584:Cytological Techniques; D006801:Humans; D010599:Pharmacokinetics; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry / Q000379:methods*; D013055:Spectrophotometry, Infrared; D013059:Spectrum Analysis, Raman