Development and validation of a rapid 96-well format based liquid-liquid extraction and liquid chromatography-tandem mass spectrometry analysis method for ondansetron in human plasma.
A rapid and sensitive LC-MS/MS method for the quantification of ondansetron was developed and validated. The plasma samples were treated by a semi-automated liquid-liquid extraction (LLE) in 1.2 mL 96-well format micro-tubes. Ondansetron and the internal standard (IS) granisetron were analyzed by combined reversed phase LC-MS/MS, with positive ion electrospray ionization, using multiple reactions monitoring (MRM). The statistical evaluation for this method reveals excellent linearity, accuracy and precision values for the range of concentrations 0.25-40.0 ng/mL. The proposed method enabled the reliable determination of ondansetron in bioequivalence studies after per os administration of a 4 or 8 mg tablet.
Dotsikas
Yannis
Y
Laboratory of Pharmaceutical Analysis and Bioequivalence Services, Department of Pharmaceutical Chemistry, School of Pharmacy, University of Athens, Greece.
Kousoulos
Constantinos
C
Tsatsou
Georgia
G
Loukas
Yannis L
YL
D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D017294:Ondansetron / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012702:Serotonin Antagonists / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D012702:Serotonin Antagonists; D017294:Ondansetron