Characterization of external electric field-driven ATP synthesis in chloroplasts.
External electric field-induced ADP phosphorylation (EFP) in lettuce chloroplasts was monitored with a coupled luciferin-luciferase enzymatic assay. This assay made it possible to follow ATP synthesis in a kinetically competent manner. The EFP reaction was found to be a much slower process than the light-driven reaction in the same system. The amount of ATP synthesized after a single electric field pulse corresponds to many turnovers of the ATP synthase.
Department of Biochemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.
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Vinkler
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Korenstein