Subdivision of the accessory olfactory bulb in the Japanese common toad, Bufo japonicus, revealed by lectin histochemical analysis.
Lectin binding patterns in the olfactory bulb of the Japanese common toad, Bufo japonicus, were examined using 21 types of lectin. Ten out of 21 lectins, WGA, s-WGA, LEL, STL, DBA, VVA, SJA, RCA-I, PNA, and PHA-L, stained the olfactory nerve, the glomeruli in the main olfactory bulb (MOB), the vomeronasal nerve, and the glomeruli in the accessory olfactory bulb (AOB). The binding patterns of LEL, STL, DBA, and PHA-L subdivided AOB glomeruli into rostral and caudal regions, where LEL, STL, and DBA stained the rostral region more intensely than the caudal region, and PHA-L had the opposite effect. Another lectin, BSL-I, stained both AOB glomeruli and the vomeronasal nerve, but not MOB glomeruli or the olfactory nerve. This is the first report of histological subdivision in the AOB of an amphibian, which suggests that the AOB development in Bufo may be unique.
Saito
Shouichiro
S
Laboratory of Veterinary Anatomy, Faculty of Applied Biological Sciences, Gifu University, 1-1Yanagido, Gifu, 501-1193, Japan. shouichi@cc.gifu-u.ac.jp
Kobayashi
Naoto
N
Atoji
Yasuro
Y
D000222:Adaptation, Physiological; D000818:Animals; D002025:Bufonidae / Q000033:anatomy & histology*; D004777:Environment; D005260:Female; D006001:Glycoconjugates; D006651:Histocytochemistry / Q000379:methods*; D007564:Japan; D037102:Lectins; D008297:Male; D009830:Olfactory Bulb / Q000033:anatomy & histology* / Q000502:physiology; D010802:Phylogeny
D006001:Glycoconjugates; D037102:Lectins