The first immunoglobulin-like domain of porcine nectin-1 is sufficient to confer resistance to pseudorabies virus infection in transgenic mice.
Nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein D (gD). Porcine nectin-1 mediates entry of pseudorabies virus (PRV), herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), and bovine herpesvirus type 1 (BHV-1). The gD-binding domain of nectin-1 is the first or N-terminal immunoglobulin (Ig)-like domain of the entire ectodomain. Here, we generated three transgenic mouse lines expressing a fusion protein consisting of the first Ig-like domain of porcine nectin-1 and the Fc portion of porcine IgG1 to assess the antiviral potential of the first Ig-like domain of nectin-1 in vivo. All of the transgenic mouse lines showed significant resistance to PRV infection via intraperitoneal inoculation (survival rates of 67% to 100%). In the intranasal challenge, a lower but still significant protection was observed; 21% to 55% of the animals from the three transgenic mouse lines survived. The present results demonstrate that a soluble form of the first domain of porcine nectin-1 is able to exert a significant antiviral effect against pseudorabies virus infection.
Ono
E
E
The Avian Zoonoses Research Centre, Faculty of Agriculture, Tottori University, Tottori, Japan. etsuro@muses.tottori-v.ac.jp
Tomioka
Y
Y
Watanabe
Y
Y
Amagai
K
K
Taharaguchi
S
S
Glenisson
J
J
Cherel
P
P
D000818:Animals; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000502:physiology*; D004195:Disease Models, Animal; D011558:Herpesvirus 1, Suid / Q000254:growth & development*; D007113:Immunity, Innate / Q000235:genetics*; D007141:Immunoglobulin Fc Fragments / Q000235:genetics; D007074:Immunoglobulin G / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D000075983:Nectins; D017434:Protein Structure, Tertiary; D011557:Pseudorabies / Q000235:genetics / Q000276:immunology / Q000821:virology*; D011991:Receptors, Virus / Q000737:chemistry / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D016019:Survival Analysis
D015815:Cell Adhesion Molecules; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; C000619015:Nectin1 protein, mouse; D000075983:Nectins; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins