Proteins pattern alteration in AZT-treated K562 cells detected by two-dimensional gel electrophoresis and peptide mass fingerprinting.
In this study we report the effect of AZT on the whole protein expression profile both in the control and the AZT-treated K562 cells, evidenced by two-dimensional gel electrophoresis and peptide mass fingerprinting analysis. Two-dimensional gels computer digital image analysis showed two spots that appeared up-regulated in AZT-treated cells and one spot present only in the drug exposed samples. Upon extraction and analysis by peptide mass fingerprinting, the first two spots were identified as PDI-A3 and stathmin, while the third one was proved to be NDPK-A. Conversely, two protein spots were present only in the untreated K562 cells, and were identified as SOD1 and HSP-60, respectively.
Department of Biochemical Sciences and Technologies, University of L'Aquila, Via Vetoio, 67100 L'Aquila, Italy. gadan@cc.univaq.it
Gabriele
G
D'Andrea
Anna R
AR
Lizzi
Sara
S
Venditti
Laura
L
Di Francesco
Alessandra
A
Giorgi
Giuseppina
G
Mignogna
Arduino
A
Oratore
Argante
A
Bozzi