[Isolation and structural elucidation of a bioactive saponin from tubers of Bolbostemma paniculatum].
OBJECTIVE
To study the bioactive constituents from tubers of Bolbostemma paniculatum.


METHODS
Compounds were isolated by extraction and partition as well as several-chromatographic techniques guided with Pyricularia oryzae bioassay method. Their structures were determined on the basis of spectral analysis and chemical evidence.


RESULTS
Bisdesmoside (I) was isolated as active compound causing morphological abnormality of Pyncularia oryzae mycelia and elucidated as 3-0-alpha-L-arabinopyranosyl (1-->2)-beta-D-glucopyranosyl-bayogenin-28-O-beta-D-xylopyranosyl (1-->3)-alpha-L-rhamnopyranosyl (1-->2)-alpha-L-arabinopyranoside.


CONCLUSIONS
I is a new natural product and exhibited significant cytotoxicity against cancer cell lines K-562 and BEL-7402, but no hemolytic activity to rabbit erythrocytes.
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. tanghaifeng71@126.com
Hai-Feng
HF
Tang
Shu-Yu
SY
Zhang
Yang-Hua
YH
Yi
Ai-Dong
AD
Wen
Yue-Ping
YP
Zhao
Zhong-Zhuang
ZZ
Wang
D000818:Animals; D001681:Biological Assay; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019662:Cucurbitaceae / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D008113:Liver Neoplasms / Q000473:pathology*; D008968:Molecular Conformation; D015394:Molecular Structure; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011817:Rabbits; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
D012503:Saponins; C511987:bisdesmoside