Characterization of two oxidatively modified phospholipids in mixed monolayers with DPPC.
The properties of two oxidatively modified phospholipids viz. 1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine (PoxnoPC) and 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PazePC), were investigated using a Langmuir balance, recording force-area (pi-A) isotherms and surface potential psi. In mixed monolayers with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) a progressive disappearance of the liquid expanded-liquid condensed transition and film expansion was observed with increasing content of the oxidized phospholipids. The above is in agreement with fluorescence microscopy of the monolayers, which revealed an increase in the liquid expanded region of DPPC monolayers. At a critical pressure pi(s) approximately 42 mN/m both Poxo- and PazePC induced a deflection in the pi-A isotherms, which could be rationalized in terms of reorientation of the oxidatively modified acyl chains into aqueous phase (adaptation of the so-called extended conformation), followed upon further film compression by solubilization of the oxidized phospholipids into the aqueous phase. Surface potential displayed a discontinuity at the same value of area/molecule, corresponding to the loss of the oxidized phospholipids from the monolayers. Our data support the view that lipid oxidation modifies both the small-scale structural dynamics of biological membranes as well as their more macroscopic lateral organization. Accordingly, oxidatively modified lipids can be expected to influence the organization and functions of membrane associated proteins.
Sabatini
Karen
K
Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Centro di Studio sui Mitocondri e Metabolismo Energetico--CNR, Bari, Italy.
Mattila
Juha-Pekka
JP
Megli
Francesco M
FM
Kinnunen
Paavo K J
PK
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D045424:Complex Mixtures / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D015227:Lipid Peroxidation; D008081:Liposomes / Q000737:chemistry*; D008560:Membrane Fluidity; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D012995:Solubility; D013329:Structure-Activity Relationship
D045424:Complex Mixtures; D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; D015060:1,2-Dipalmitoylphosphatidylcholine; D010100:Oxygen