[Clustered shoot induction and rapid propagation on Pinellia ternate].
OBJECTIVE
To establish the rapid propagation system of Pinellia ternate.


METHODS
The Clustered shoots were induced by the explant lamina and petiole from the terminal bud of Pinellia ternate tuber in test tube.


RESULTS
The plantlets could regenerate from the lamina and petiole directly without via the callus. The MS Medium with 2.0 mg/L 6-BA and 0.1 mg/L NAA was optimal for the induction of culstered shoots from explants of lamina; the MS with 2.0 mg/L 6-BA and 0.25 mg/L NAA was best for the explants of petiole; and the 1/2 MS with 1.0 mg/L KT and 2.0 mg/L NAA was the better medium for induction of roots.


CONCLUSIONS
The regenerated rate of Pinellia ternata can improve by inducing the clustered shoots from the explants of lamina and petiole directly.
School of Life Sciences, Southwest University, Chongqing.
Xiumei
X
Xu
Daoxi
D
Lu
Min
M
Sun
Ming
M
Zhang
Min
M
Chen
D003470:Culture Media; D030021:Pinellia / Q000187:drug effects / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010937:Plant Growth Regulators