Toward a physical map of the genome of the nematode Caenorhabditis elegans.
A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described. The technique is being applied to fragments from the nematode Caenorhabditis elegans (i) to facilitate cross-indexing of clones emanating from different laboratories and (ii) to construct a physical map of the genome. Eight hundred sixty clusters of clones, from 35 to 350 kilobases long and totaling about 60% of the genome, have been characterized.
Coulson
A
A
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England.
Sulston
J
J
Brenner
S
S
Karn
J
J