Systematic comparison of two methods to measure parasite density from malaria blood smears.
This study was designed to directly compare the accuracy, reproducibility, and efficiency of three methods commonly used to measure blood-stage malaria parasite density from Giemsa-stained blood films. Parasites and white blood cells (WBCs) were counted in 154 thick films by two independent microscopists. Forty-six slides were read by counting parasitized red blood cells (RBCs) in the thin film. Using these same slides, parasites were again counted by two independent microscopists using an ocular grid. Overall, parasite densities were significantly lower and discrepancy between readers was higher when using the grid method compared to the WBC method, but there was no difference when compared to the RBC method. When one reader who had difficulty with the grid method was excluded, the discrepancy between readers was equivalent for the three methods. Densities and discrepancy between readers were indistinguishable when parasites were counted until 200 or 500 WBCs. Counting beyond 200 WBCs may not significantly improve parasite density measurements. Using an ocular grid directly measures parasites per volume rather than using a WBC per microliter conversion factor and eliminates the need to switch from the thick film to the thin film for high parasitemias. However, significant differences in densities measured by the grid method and the WBC method need to be evaluated.
Fogarty International Center, NIH, Bethesda, MD, USA. prudhomw@mail.nih.gov
Wendy
W
Prudhomme O'Meara
Shon
S
Remich
Bernhards
B
Ogutu
Martin
M
Lucas
Ramadan
R
Mtalib
Peter
P
Obare
Frederick
F
Oloo
Caroline
C
Onoka
Joseph
J
Osoga
Colin
C
Ohrt
F Ellis
FE
McKenzie
D000818:Animals; D001399:Azure Stains; D004906:Erythrocyte Count; D007958:Leukocyte Count; D016778:Malaria, Falciparum / Q000097:blood / Q000175:diagnosis / Q000469:parasitology*; D008853:Microscopy / Q000379:methods*; D010963:Plasmodium falciparum / Q000302:isolation & purification*; D015203:Reproducibility of Results
D001399:Azure Stains