Antigen challenge inhibits thymic emigration.
T cell development in the thymus involves a series of TCR-mediated control points including TCR-beta selection and positive and negative selection. Approximately half of the thymic sojourn is spent in the medulla, where thymocytes undergo final maturation before emigrating to the periphery. Although it is acknowledged that thymic emigration is an active process, relatively little is known about how this is regulated, why it takes so long, and whether TCR-mediated signaling can influence this step. Using wild-type and TCR transgenic mice, we found that Ag injected i.v. or intrathymically led to a striking reduction in the number of recent thymic emigrants (RTE) in the periphery. This was caused by inhibition of T cell export rather than peripheral deletion, because a cohort of RTE that was already released before in vivo Ag challenge was not depleted, and similar results were observed in Bim-deficient mice, which have impaired T cell deletion. Within the thymus, the loss of RTE was associated with retention of medullary thymocytes rather than increased negative selection. In addition to Ag-specific inhibition of export, some TCR-independent suppression of emigration was also observed that appeared to be partly the result of the inflammatory cytokine TNF. Thus, in addition to its accepted role in intrathymic selection events, TCR signaling can also play an important role in the regulation of thymic emigration.
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia. adam.uldrich@petermac.org
Adam P
AP
Uldrich
Stuart P
SP
Berzins
Mark A
MA
Malin
Philippe
P
Bouillet
Andreas
A
Strasser
Mark J
MJ
Smyth
Richard L
RL
Boyd
Dale I
DI
Godfrey
D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D002465:Cell Movement / Q000276:immunology; D004768:Enterotoxins / Q000008:administration & dosage / Q000276:immunology; D007272:Injections, Intralymphatic; D007275:Injections, Intravenous; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D018089:Superantigens / Q000008:administration & dosage; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*
D000941:Antigens; D004768:Enterotoxins; D011948:Receptors, Antigen, T-Cell; D018089:Superantigens; C034959:enterotoxin C, staphylococcal