Culture conditions improving the production of jadomycin B.
The jadomycins are a unique family of benzoxazolophenanthridine antibiotics produced by Streptomyces venezuelae ISP5230 following heat or ethanol shock or phage infection. We have modified the culture conditions by altering the carbon source, buffer, inoculum size, and timing of ethanol shock, thereby reducing growing times and improving jadomycin B production. Our optimized conditions use glucose as the carbon source, MOPS as buffer, low concentrations of phosphate, a defined inoculum concentration and an immediate ethanol shock to induce jadomycin B production; results that contrast previous studies. The altered media will facilitate the isolation of related jadomycin B congeners.
College of Pharmacy, Dalhousie University, Halifax, NS, Canada. david.jakeman@dal.ca
David L
DL
Jakeman
Cathy L
CL
Graham
Wendy
W
Young
Leo C
LC
Vining
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism; D001431:Bacteriological Techniques / Q000379:methods*; D001709:Biotechnology / Q000379:methods*; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D007546:Isoquinolines / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D009025:Morpholines / Q000737:chemistry / Q000378:metabolism; D009285:Naphthoquinones / Q000737:chemistry / Q000378:metabolism; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D011508:Chondroitin Sulfate Proteoglycans; D003470:Culture Media; D007546:Isoquinolines; D009025:Morpholines; D009285:Naphthoquinones; C506597:jadomycin A; C081750:jadomycin B; C008550:morpholinopropane sulfonic acid; D000431:Ethanol; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D005947:Glucose