Reevaluation of the resting time period when preparing whole blood-derived platelet concentrates with the platelet-rich plasma method.
BACKGROUND
The resting of platelet (PLT) pellets during the preparation of whole blood-derived PLT concentrates (PCs) is considered an essential step. A reevaluation of the rest period was conducted because preparation and storage conditions have been modified during the past 20 years.


METHODS
A two-site in vitro study (Study 1) was conducted with rest times of 0 to 5 minutes, 1 hour, and 4 hours (n = 31-33 per rest period). Leukoreduced PCs were stored for 5 days. A second study (Study 2) measuring in vivo viability was conducted at a third site (14 paired studies). PCs were prepared from 2 units of whole blood on the same day to simultaneously compare viability following storage and radioisotopic labeling.


RESULTS
In Study 1, comparable in vitro parameters and swirling were observed with the three rest periods. The mean (+/-1 SD) values after storage for the extent of shape change and hypotonic stress parameters were 0 to 5 minutes, 16.7 +/- 7.2 and 66.0 +/- 15.7%; 1 hour, 19.1 +/- 6.9 and 69.3 +/- 17.9%; and 4 hours, 17.6 +/- 5.5 and 64.1 +/- 11.3%. In Study 2, the in vivo recovery was 49.9 +/- 15.3 and 50.9 +/- 20.2% with 0- to 5-minute and 1-hour rest periods, respectively. The corresponding survival time was 111.2 +/- 50.7 and 114.9 +/- 43.8 hours.


CONCLUSIONS
These studies indicate comparable in vitro and in vivo viability properties with 0- to 5-minute and 1-hour rest periods and at 4 hours (in vitro only).
Moroff
Gary
G
American Red Cross, Jerome Holland Laboratory for the Biomedical Sciences, Rockville, MD 20855, USA. Moroff@usa.redcross.org
Kline
Linda
L
Dabay
Michelle
M
Hunter
Suzanne
S
Johnson
Adrienne
A
McNeil
Deanna
D
Nixon
Jan
J
Sawyer
Sherrie
S
Taylor
Harry
H
Whitley
Pam
P
Wahab
Fawzi
F
Murphy
Scott
S
D000925:Anticoagulants; D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation / Q000379:methods; D002245:Carbon Dioxide / Q000097:blood; D002470:Cell Survival; D006801:Humans; D066298:In Vitro Techniques; D010100:Oxygen / Q000097:blood; D010976:Platelet Count; D017713:Platelet Transfusion / Q000379:methods*; D013997:Time Factors
D000925:Anticoagulants; D002245:Carbon Dioxide; D010100:Oxygen