Antinociceptive activity of 3-O-beta-d-glucopyranosyl-23,24-dihydrocucurbitacin F from Hintonia standleyana (Rubiaceae).
Oral administration of a MeOH-CH(2)Cl(2) (1:1) extract of the stem bark of Hintonia standleyana (HSE) produced a dose-dependent antinociceptive effect when tested in mice using the writhing (150-750 mg/kg) and the hot-plate (150-600 mg/kg) models. From the active extract 3-O-beta-d-glucopyranosyl-23,24-dihydrocucurbitacin F (GDHCF), 5-O-[beta-d-apiofuranosyl-(1-->6)-beta-d-glucopyranosyl]-7-methoxy-3',4'-dihydroxy-4-phenylcoumarin (AG4-PC) and desoxycordifolinic acid (DCA) were isolated. GDHCF (10-100 mg/kg, p.o.) significantly reduced acetic acid-induced abdominal contortions and increased the hot-plate latency in comparison to vehicle-treated mice. Metamizol (50-100 mg/kg) and morphine (2.5-5 mg/kg) were used as positive controls, respectively. GDHCF-induced antinociception was partially blocked by naloxone (1 mg/kg, i.p.), l-NAME (150 mg/kg, i.p.) and glibenclamide (10mg/kg, i.p.) suggesting that its pharmacological effect could be due to the activation of the nitric oxide pathway, followed by the opening of the ATP-sensitive K(+) channels, as well as an activation of the opioid receptors.
Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca Morelos 1210, Mexico.
Myrna
M
Déciga-Campos
José A
JA
Guerrero-Analco
Leovigildo
L
Quijano
Rachel
R
Mata
D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D004177:Dipyrone / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D009020:Morphine / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019666:Rubiaceae / Q000737:chemistry*; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013057:Spectrum Analysis / Q000379:methods
C512338:3-O-glucopyranosyl-23,24-dihydrocucurbitacin F; D000700:Analgesics; D012503:Saponins; D004177:Dipyrone; D009020:Morphine; D005905:Glyburide; D019331:NG-Nitroarginine Methyl Ester