Estrogen-like activity of seafood related to environmental chemical contaminants.
BACKGROUND
A wide variety of environmental pollutants occur in surface waters, including estuarine and marine waters. Many of these contaminants are recognised as endocrine disrupting chemicals (EDCs) which can adversely affect the male and female reproductive system by binding the estrogen receptor and exhibiting hormone-like activities. In this study the estrogenic activity of extracts of edible marine organisms for human consumption from the Mediterranean Sea was assayed.


METHODS
Marine organisms were collected in two different areas of the Mediterranean Sea. The estrogenic activity of tissues was assessed using an in vitro yeast reporter gene assay (S. cerevisiae RMY 326 ER-ERE). Concentrations of polychlorinated biphenyls (PCBs) (congeners 28, 52, 101, 118, 138, 153, 180) in fish tissue was also evaluated.


RESULTS
Thirty-eight percent of extracts showed a hormone-like activity higher than 10% of the activity elicited by 10 nM 17b-estradiol (E2) used as control. Total PCB concentrations ranged from 0.002 up to 1.785 ng/g wet weight. Chemical analyses detected different levels of contamination among the species collected in the two areas, with the ones collected in the Adriatic Sea showing concentrations significantly higher than those collected in the Tyrrhenian Sea (p < 0.01).


CONCLUSIONS
The more frequent combination of chemicals in the samples that showed higher estrogenic activity was PCB 28, PCB 101, PCB 153, PCB 180. The content of PCBs and estrogenic activity did not reveal any significant correlation.
IARC, 150 Cours Albert-Thomas, 69372 Lyon Cedex 08, France. garritanos@iarc.fr
Sonia
S
Garritano
Barbara
B
Pinto
Marco
M
Calderisi
Teresa
T
Cirillo
Renata
R
Amodio-Cocchieri
Daniela
D
Reali
D000818:Animals; D003445:Crustacea; D004967:Estrogens; D005260:Female; D005399:Fishes; D008297:Male; D008522:Mediterranean Sea; D011078:Polychlorinated Biphenyls / Q000009:adverse effects / Q000032:analysis*; D017747:Seafood; D014874:Water Pollutants, Chemical / Q000009:adverse effects / Q000032:analysis*; D001616:beta-Galactosidase / Q000378:metabolism
D004967:Estrogens; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D001616:beta-Galactosidase