Pancreatobiliary afferent recordings in the anaesthetised Australian possum.
The sensory innervation to the pancreatobiliary system is poorly characterized. Afferent signals from the gastrointestinal tract and biliary tree are transmitted to the central nervous system via the vagus and spinal nerves. We aimed to record afferent discharge in order to characterize the vagal and splanchnic afferent signals from the possum upper gastrointestinal tract, biliary tree and pancreas. In 21 anaesthetised possums nerve fibres were teased from the vagus or splanchnic nerve for multi-unit recording. Mechanical stimuli consisted of balloon distension of the gallbladder and duodenum (2-7 ml) and fluid distension (0-20 mm Hg) of the bile or pancreatic ducts. Approximately 60% of fibres from all nerves displayed spontaneous discharge. Spinal afferent responses to mechanical stimuli were infrequent (n=13). Increased discharge occurred in response to duodenal (12/99 fibres) or gallbladder (7/96 fibres) distension, but not to bile duct (0/73 fibres) or pancreatic duct (0/51 fibres) distension. Vagal afferent responses to distension of the duodenum or stomach (5-30 ml) were more common (n=8). Increased discharge was recorded in response to duodenal (49/134 fibres), or gastric (22/70 fibres) distension. Responses to gallbladder distension were less frequent (6/99 fibres) and as with the spinal afferent no response to bile duct (0/66) or pancreatic duct (0/70) distension were recorded. We conclude that mechanosensitive afferents in the pancreatobiliary system are relatively rare, particularly within the ducts, and/or that they are adapted to monitor stimuli other than luminal distension.
Department of General and Digestive Surgery, Centre for Neuroscience, Flinders University, Flinders Medical Centre, Bedford Park, and Department Medicine, Royal Adelaide Hospital, S.A., 5042, Australia.
A C
AC
Schloithe
C M
CM
Woods
J S
JS
Davison
L A
LA
Blackshaw
J
J
Toouli
G T P
GT
Saccone
D000200:Action Potentials / Q000502:physiology; D000758:Anesthesia; D000818:Animals; D003135:Common Bile Duct / Q000294:innervation / Q000502:physiology*; D005260:Female; D005769:Gastrointestinal Motility / Q000502:physiology; D041981:Gastrointestinal Tract / Q000294:innervation / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology*; D010183:Pancreatic Ducts / Q000294:innervation / Q000502:physiology*; D010812:Physical Stimulation / Q000379:methods; D013153:Splanchnic Nerves / Q000502:physiology*; D048330:Trichosurus / Q000502:physiology; D014630:Vagus Nerve / Q000502:physiology*