Three-layer matrix/sample preparation method for MALDI MS analysis of low nanomolar protein samples.
A robust and sensitive sample preparation method is presented for matrix-assisted laser desorption ionization (MALDI) mass spectrometric analysis of low nanomolar concentrations of proteins containing high amounts of common salts and buffers. This method involves the production of densely packed sub-micrometer matrix crystals by depositing a matrix solution on top of a matrix seed-layer prepared on a MALDI target. A sub-microliter aliquot of analyte solution is then directly added to the top of the matrix crystals to form a thin-layer. alpha-Cyano-4-hydroxycinnamic acid (4-HCCA) is used as matrix and demonstrated to give better performance than other commonly used matrices, such as 2,5-dihydroxybenzoic acid (DHB), 2-(4-hydroxy-phenylazo) benzoic acid (HABA), or sinapinic acid. This three-layer method is shown to be superior to the other MALDI sample preparation methods, particularly for handling low nanomolar protein solutions containing salts and buffers.
Keller
Bernd O
BO
Department of Chemistry, University of Alberta, E3-44 Chemistry Bldg., T6G 2G2, Edmonton, Alberta, Canada.
Li
Liang
L
Department of Chemistry, University of Alberta, E3-44 Chemistry Bldg., T6G 2G2, Edmonton, Alberta, Canada. liang.li@ualberta.ca.
D000818:Animals; D001436:Bacteriorhodopsins / Q000032:analysis*; D002417:Cattle; D003373:Coumaric Acids; D007781:Lactoferrin / Q000032:analysis*; D036103:Nanotechnology; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D003373:Coumaric Acids; C007175:alpha-cyano-4-hydroxycinnamate; D001436:Bacteriorhodopsins; D007781:Lactoferrin