Syndecan-2 is expressed in the microvasculature of gliomas and regulates angiogenic processes in microvascular endothelial cells.
Angiogenesis is the formation of new blood vessels from the existing vasculature and is necessary for tumor growth. Syndecan-2 (S2) is highly expressed in the microvasculature of mouse gliomas. When S2 expression was down-regulated in mouse brain microvascular endothelial cells (MvEC), this inhibited cell motility and reduced the formation of capillary tube-like structures in vitro. Pro-angiogenic growth factors and enzymes up-regulated during glioma tumorigenesis stimulated shedding of the S2 ectodomain from endothelial cells in vitro. The effect of shed S2 on angiogenic processes was investigated by incorporating recombinant S2 ectodomain (S2ED) into in vitro angiogenesis assays. S2ED promoted membrane protrusion, migration, capillary tube formation, and cell-cell interactions. We therefore propose that S2 is necessary for angiogenesis of MvEC, proangiogenic factors expressed during glioma progression regulate S2 shedding, and shed S2 ectodomain may increase endothelial cell angiogenic processes.
Department of Cell Biology, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham, AL 35294-0006, USA.
Constance Y
CY
Fears
Candece L
CL
Gladson
Anne
A
Woods
D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism*; D002417:Cattle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004730:Endothelium, Vascular / Q000378:metabolism*; D005786:Gene Expression Regulation; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D008833:Microcirculation; D009389:Neovascularization, Pathologic; D011509:Proteoglycans / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000737:chemistry; D053669:Syndecan-2
D008562:Membrane Glycoproteins; D011509:Proteoglycans; D011994:Recombinant Proteins; C508245:SDC2 protein, human; C508246:Sdc2 protein, mouse; D053669:Syndecan-2