Aquaporin-1 in the peritoneal membrane: Implications for water transport across capillaries and peritoneal dialysis.
Peritoneal dialysis (PD) is an established mode of renal replacement therapy, based on the exchange of fluid and solutes between blood in peritoneal capillaries and a dialysate that has been introduced in the peritoneal cavity. The dialysis involves diffusive and convective transports and osmosis through the highly vascularized peritoneal membrane. Computer simulations predicted that the membrane contains ultrasmall pores (radius < 3 A) responsible for the transport of solute-free water across the capillary endothelium during crystalloid osmosis. The distribution of the water channel aquaporin-1 (AQP1), as well as its molecular structure ensuring an exquisite selectivity for water perfectly fit with the characteristics of the ultrasmall pore. Treatment with corticosteroids induces the expression of AQP1 in peritoneal capillaries and increases water permeability and ultrafiltration in rats, without affecting the osmotic gradient and the permeability for small solutes. Studies in knockout mice provided further evidence that osmotically-driven water transport across the peritoneal membrane is mediated by AQP1. AQP1 and endothelial NO synthase (eNOS) show a distinct regulation within the endothelium lining peritoneal capillaries. In acute peritonitis, the upregulation of eNOS and increased release of NO dissipate the osmotic gradient and result in ultrafiltration failure, despite the unchanged expression of AQP1. These data illustrate the potential of the peritoneal membrane to investigate the role and regulation of AQP1 in the endothelium. They also emphasize the critical role of AQP1 during peritoneal dialysis and suggest that manipulating AQP1 expression may be used to increase water permeability across the peritoneal membrane.
Devuyst
Olivier
O
Division of Nephrology, Université catholique de Louvain Medical School, 10 Avenue Hippocrate, B-1200 Brussels, Belgium. devuyst@nefr.ucl.ac.be
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Jie
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D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism / Q000502:physiology*; D001693:Biological Transport, Active; D002196:Capillaries / Q000378:metabolism / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000378:metabolism; D006801:Humans; D007676:Kidney Failure, Chronic / Q000503:physiopathology / Q000628:therapy; D051379:Mice; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D009995:Osmosis; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000098:blood supply*; D014867:Water / Q000378:metabolism*
D014867:Water; D051398:Aquaporin 1; D052250:Nitric Oxide Synthase Type III