From yeast ammonium transporters to Rhesus proteins, isolation and functional characterization.
Saccharomyces cerevisiae possesses three ammonium transporters from the Mep/Amt family involved in ammonium acquisition and retention. We have shown that Rh proteins are structurally related to Mep/Amt proteins and that human RhAG and RhCG perform bi-directional ammonium transport upon heterologous expression in yeast. Using yeast as an expression tool, we have started a structure-function analysis of distinct members from the Mep/Amt/Rh super-family.
Laboratoire de physiologie moléculaire de la cellule, institut de biologie et de médecine moléculaires, université libre de Bruxelles CP300, rue des professeurs-Jeener-et-Brachet, 12, 6041 Gosselies, Belgium. ammarini@dbm.ulb.ac.be
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Marini
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Boeckstaens
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André
D000818:Animals; D001798:Blood Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D027682:Cation Transport Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000502:physiology; D006801:Humans; D007448:Invertebrates / Q000378:metabolism; D017136:Ion Transport; D008562:Membrane Glycoproteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D010940:Plant Proteins / Q000302:isolation & purification / Q000502:physiology; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000302:isolation & purification / Q000502:physiology*; D013045:Species Specificity; D013379:Substrate Specificity
C111096:AmtB protein, E coli; D001798:Blood Proteins; D027682:Cation Transport Proteins; D029968:Escherichia coli Proteins; C088951:MEP1 protein, S cerevisiae; C107270:MEP2 protein, S cerevisiae; C107271:MEP3 protein, S cerevisiae; D008562:Membrane Glycoproteins; D010940:Plant Proteins; D000644:Quaternary Ammonium Compounds; C498523:RHAG protein, human; C413213:RHCG protein, human; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C088952:ammonium transporters, plant