Clostridium ganghwense sp. nov., isolated from tidal flat sediment.
A Gram-negative, strictly anaerobic, halophilic, motile, sporulating and rod-shaped bacterium, designated strain HY-42-06T, was isolated from tidal flat sediment from Ganghwa Island in South Korea. The isolate produced glycerol, ethanol and CO2 as fermentation end-products from glucose. Strain HY-42-06T grew optimally at 35 degrees C, pH 7.5 and 3 % (w/v) artificial sea salts. No growth was observed in the absence of sea salts. In phylogenetic analyses based on 16S rRNA gene sequence, strain HY-42-06T showed a distinct phyletic line within the members of cluster I of the order Clostridiales. The closest phylogenetic neighbour to strain HY-42-06T was Clostridium novyi ATCC 17861T (94.91 % 16S rRNA gene sequence similarity). Several phenotypic characters readily differentiate the tidal flat isolate from phylogenetically related clostridia. On the basis of polyphasic evidence, strain HY-42-06T should be classified as a representative of a novel species, for which the name Clostridium ganghwense sp. nov. is proposed. The type strain is HY-42-06T (=IMSNU 40127T = KCTC 5146T = JCM 13193T).
Kim
Seil
S
School of Biological Sciences and Institute of Microbiology, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Jeong
Hyunyoung
H
School of Biological Sciences and Institute of Microbiology, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Kim
Sanggoo
S
School of Biological Sciences and Institute of Microbiology, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Chun
Jongsik
J
School of Biological Sciences and Institute of Microbiology, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
D003013:Clostridium / Q000145:classification / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D013171:Spores, Bacterial / Q000166:cytology; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S