New methods for calibration of the RF field strength for indirectly observed nuclei.
Two novel pulse sequences, CALIS-1 and CALIS-2, for accurate calibration of the RF field strength for an indirectly observed spin are introduced. CALIS-2 is intended for calibration of e.g., (13)C or (15)N pulses on natural abundance samples whilst CALIS-1 is recommended primarily for enriched samples. Both experiments can be performed without prior knowledge or guess of the RF field strength and no delays in the pulse sequences are critically dependent on coupling constants.
Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark.
Andrew J
AJ
Benie
Ole W
OW
Sørensen
D002138:Calibration; D002247:Carbon Isotopes; D008358:Mannose / Q000737:chemistry; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000295:instrumentation*; D010455:Peptides / Q000737:chemistry*; D010940:Plant Proteins; D011846:Radio Waves
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D010455:Peptides; D010940:Plant Proteins; C093336:chymotrypsin inhibitor 2; D008358:Mannose