Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT.
Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and results indicated that tetratricopeptide repeat 2 (aa 125-158) was essential for interaction. We also showed that 7a interacted with SARS-CoV structural proteins M (membrane) and E (envelope), which have been shown to be essential for virus-like particle formation. Taken together, our results coupled with data from studies of the interaction between SGT and HIV-1 vpu indicated that SGT could be involved in the life-cycle, possibly assembly of SARS-CoV.
Fielding
Burtram C
BC
Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos Building, Singapore 138673, Singapore. bfielding@uwc.ac.za
Gunalan
Vithiagaran
V
Tan
Timothy H P
TH
Chou
Chih-Fong
CF
Shen
Shuo
S
Khan
Sehaam
S
Lim
Seng Gee
SG
Hong
Wanjin
W
Tan
Yee-Joo
YJ
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D045473:Severe acute respiratory syndrome-related coronavirus; D020798:Two-Hybrid System Techniques; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000378:metabolism*
D002352:Carrier Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; C114575:SGTA protein, human; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C488151:sars7a protein, SARS virus