Validation of liquid/liquid extraction method coupled with HPLC-UV for measurement of ribavirin plasma levels in HCV-positive patients.
Measurement of ribavirin plasma levels in HCV-positive patients have been shown to be useful in order to optimise individual ribavirin exposure. Efficacy and toxicity of this drug are shown to be concentration-dependant. A simple HPLC-UV method was developed and validated, which has an easy liquid/liquid extraction, sensitive limit of detection, without any interference peaks, reproducible and linear over the range of clinical relevant concentrations. The assay warrants further evaluation as a tool for ribavirin therapeutic drug monitoring in HCV-positive patients.
D'Avolio
Antonio
A
Department of Infectious Diseases, University of Torino, Amedeo di Savoia Hospital, Corso Svizzera, 164-10149 Turin, Italy. antodav@inwind.it
Ibañez
Allende
A
Sciandra
Mauro
M
Siccardi
Marco
M
de Requena
Daniel Gonzalez
DG
Bonora
Stefano
S
Di Perri
Giovanni
G
D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D019698:Hepatitis C, Chronic / Q000097:blood; D006801:Humans; D015203:Reproducibility of Results; D012254:Ribavirin / Q000097:blood*; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
D012254:Ribavirin