Engineering of protein thermodynamic, kinetic, and colloidal stability: Chemical Glycosylation with monofunctionally activated glycans.
In this work we establish the relationship between chemical glycosylation and protein thermodynamic, kinetic, and colloidal stability. While there have been reports in the literature that chemical glycosylation modulates protein stability, mechanistic details still remain uncertain. To address this issue, we designed and coupled monofunctional activated glycans (lactose and dextran) to the model protein alpha-chymotrypsin (alpha-CT). This resulted in a series of glycoconjugates with variations in the glycan size and degree of glycosylation. Thermodynamic unfolding, thermal inactivation, and temperature-induced aggregation experiments revealed that chemical glycosylation increased protein thermodynamic (Delta G(25 degrees C)), kinetic (t(1/2)(45 degrees C)), and colloidal stability. These results highlight the potential of chemical glycosylation with monofunctional activated glycans as a technology for increasing the long-term stability of liquid protein formulations for industrial and biotherapeutic applications.
Department of Chemistry, University of Puerto Rico, Río Piedras Campus, San Juan 23346, Puerto Rico 00931-3346.
Ricardo J
RJ
Solá
Wasfi
W
Al-Azzam
Kai
K
Griebenow
D002616:Chemical Engineering / Q000379:methods*; D002918:Chymotrypsin / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D003911:Dextrans / Q000737:chemistry*; D004355:Drug Stability; D006031:Glycosylation; D007700:Kinetics; D007785:Lactose / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics
D003102:Colloids; D003911:Dextrans; D011134:Polysaccharides; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D007785:Lactose