[Studies on liquid fermentation of anamorph of Shiraia bambusicola].
A asexuae strain producing effective component hypocrellin was isolated from the hypha of Shiraia bambusicola sporo-carp. By liquid-state fermentation and orthogonal teat with shake flask culture, the optimum medium component and culture conditions were: saccharose 20 g/L, yeast extract 8 g/L, maize extract 2 g/L, pH 6.0, culture broth quantity 50/250 mL(V/V) flask, inoculum 10% (V/V), culture temperature 28 degrees C, shake rotation 130 r/min, fermentation period 96 h. The culture can produce 8 g Hypocrellin (E 1 cm (1%) 465 = 52) per liter fermentation broth. This study will provide basis for the future massively industrialized hypcorllin production.
Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, Jiangsu.
Jiajia
J
Chen
Zhaolan
Z
Li
Qingcai
Q
Jiao
D000704:Analysis of Variance; D001203:Ascomycota / Q000737:chemistry*; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D025301:Hyphae / Q000254:growth & development; D009584:Nitrogen / Q000378:metabolism; D010569:Perylene / Q000031:analogs & derivatives* / Q000302:isolation & purification; D019800:Phenol; D011809:Quinones / Q000302:isolation & purification*; D013696:Temperature
D003470:Culture Media; D011809:Quinones; D019800:Phenol; D010569:Perylene; D009584:Nitrogen; C048388:hypocrellin A