Metal-enhanced fluorescence-based RNA sensing.
In this communication, we present the proof-of-principle of a new RNA sensing technique, based on metal-enhanced fluorescence, which may improve the potential of solid substrate-based RNA capture assays. In this regard, the detection of RNA is accomplished by annealing a target RNA tagged with a fluorophore to a DNA anchor probe in a single step on a solid surface, where the fluorescence signal is enhanced by silver nanoparticles. Furthermore, the proposed method is highly amenable to high-throughput screening applications and is capable of detecting less than 25 fmol of RNA at a signal-to-noise ratio greater than 20, in several hours compared to that of reverse transcription-PCR at >24 h.
Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, University of Maryland School of Medicine, Baltimore, 21201, USA.
Kadir
K
Aslan
Jun
J
Huang
Gerald M
GM
Wilson
Chris D
CD
Geddes
D015374:Biosensing Techniques / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D012313:RNA / Q000032:analysis*; D012235:Rhodamines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
C114557:5-carboxytetramethylrhodamine succinimidyl ester; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D012235:Rhodamines; D012834:Silver; D012313:RNA