Regulation of transduction efficiency by pegylation of baculovirus vector in vitro and in vivo.
In this study, poly(ethylene glycol) (PEG) was coupled to baculovirus to regulate transduction efficiency of baculovirus in vitro and in vivo. The degree of pegylation in virions was measured by the loss of free amines via a fluorescamine-based assay. The efficiency of green fluorescent protein (GFP) expression was used to monitor transduction efficiency. As the results, the transduction efficiency in pegylated baculovirus was decreased with an increase of pegylation in baculovirus in vitro and in vivo. Interestingly, the transduction efficiency of the pegylated baculovirus for the lung and brain was increased compared with baculovirus itself possibly owing to increased stability of baculovirus by pegylation.
Kim
You-Kyoung
YK
School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea.
Park
In-Kyu
IK
Jiang
Hu-Lin
HL
Choi
Jae-Young
JY
Je
Yeon-Ho
YH
Jin
Hua
H
Kim
Hyun-Woo
HW
Cho
Myung-Haing
MH
Cho
Chong-Su
CS
D000818:Animals; D016367:Baculoviridae / Q000737:chemistry / Q000235:genetics*; D002460:Cell Line; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics* / Q000493:pharmacokinetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism / Q000493:pharmacokinetics; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000493:pharmacokinetics; D014018:Tissue Distribution / Q000187:drug effects; D014162:Transfection / Q000379:methods*
D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols