Postoperative changes of sperm chromatin heterogeneity, using acridine orange staining, in varicocele patients.
The aim of this study was to evaluate postoperative changes in sperm chromatin heterogeneity in varicocele patients. In 15 infertile patients with varicocele, sperm parameters including concentration, motility, and morphology were evaluated before and after surgical correction of varicocele. Sperm motion analysis using computer-assisted semen analyzer (CASA) was also performed. To analyze the sperm nuclear proteins, the acridine orange staining method was used. On semen analysis, sperm concentration and motility significantly increased after surgery (p = 0.002, p = 0.003, respectively), although sperm morphology was unaltered postoperatively. CASA parameters, including velocity, linearity, amplitude of lateral head displacement and beat cross frequency were unaltered postoperatively. On the other hand, acridine orange staining significantly increased postoperatively (p = 0.002). Varicocele influences the sperm chromatin condition, as well as sperm concentration and motility.
Department of Urology, Faculty of Medicine, National University Corporation, University of Toyama, Toyama, Japan. fuse@ms.toyama-mpu.ac.jp
H
H
Fuse
T
T
Akashi
I
I
Mizuno
T
T
Nozaki
A
A
Watanabe
D000165:Acridine Orange / Q000378:metabolism*; D000328:Adult; D002843:Chromatin / Q000378:metabolism / Q000473:pathology*; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D011184:Postoperative Period; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology*; D014646:Varicocele / Q000473:pathology* / Q000601:surgery
D002843:Chromatin; D005456:Fluorescent Dyes; D000165:Acridine Orange