Comparison of methods for the exhaustive extraction of hypericins, flavonoids, and hyperforin from Hypericum perforatum L.
Renewed interest in plant-derived drugs has led to an increased need for efficient extraction methods. Hypericum perforatum L. contains several groups of bioactive compounds with noteworthy pharmacological activities. Direct sonication of H. perforatum was investigated and compared with conventional maceration, indirect sonication, Soxhlet extraction, and accelerated solvent extraction (ASE). Highly selective liquid chromatography/tandem mass spectrometry analysis showed that the content of six investigated active compounds (hypericin, pseudohypericin, hyperoside, rutin, quercitrin, and hyperforin) in extracts obtained by direct sonication was significantly higher than in extracts obtained by the other methods. The active compound contents increased on increasing the ultrasonic power from 40 to 60 W when using direct sonication. Conventional maceration gave the lowest amount of analyzed active compounds. Soxhlet extraction gave better results than ASE or indirect sonication.
Institute of Environmental Research (INFU), University of Dortmund, Otto-Hahn-Strasse 6, D-44221 Dortmund, Germany.
Andrija
A
Smelcerovic
Michael
M
Spiteller
Sebastian
S
Zuehlke
D000873:Anthracenes; D001643:Bridged Bicyclo Compounds / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000302:isolation & purification*; D020902:Hypericum / Q000737:chemistry*; D013058:Mass Spectrometry; D010569:Perylene / Q000031:analogs & derivatives* / Q000302:isolation & purification; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000302:isolation & purification; D013010:Sonication; D013729:Terpenes / Q000302:isolation & purification*
D000873:Anthracenes; D001643:Bridged Bicyclo Compounds; D005419:Flavonoids; D013729:Terpenes; D010569:Perylene; C004965:hypericin; D010696:Phloroglucinol; C001654:hyperforin