Marx line: fluorescein staining line on the inner lid as indicator of meibomian gland function.
OBJECTIVE
To determine whether the location of a fluorescein-stained line, the Marx line (ML), which runs along the inner eyelid, is correlated with meibomian gland function.


METHODS
Prospective observational case series.


METHODS
After applying fluorescein dye solution to the eye, the ML score was calculated for the outer, middle, and inner thirds of the lower eyelid margin. ML scoring was as follows: 0, entirely on the conjunctival side of the meibomian orifices (MOs); 1, part of the ML touches the MOs; 2, ML runs through all of the MOs; and 3, ML runs on the eyelid-margin side of the MOs. Correlations were calculated between the total ML score and age for 251 randomly recruited subjects without acute ocular surface diseases, and between age and the ML score for the three regions of the lower eyelid. Correlations between the regional ML score and the meibographic score, and the meibomian gland secretion score were also determined. The total ML scores of 15 subjects without meibomian gland dysfunction (MGD) were compared with 15 age-matched patients with MGD.


RESULTS
The three regions of the lower eyelid had significantly different ML scores. Strong correlations were found between the ML score and age, the meibographic score, and the meibomian gland secretion score. The total ML score of MGD group was significantly higher than that of the non-MGD group.


CONCLUSIONS
The strong correlation between the ML score and the meibomian gland scores indicates that the ML score can be used as a simple and rapid screening score for meibomian gland function.
Yamaguchi
Masahiko
M
Department of Ophthalmology, University of Ehime School of Medicine, Ehime, Japan. masahiko@m.ehime-u.ac.jp
Kutsuna
Miki
M
Uno
Toshihiko
T
Zheng
Xiaodong
X
Kodama
Toshio
T
Ohashi
Yuichi
Y
D000328:Adult; D015352:Dry Eye Syndromes / Q000175:diagnosis; D005143:Eyelids / Q000502:physiology*; D005260:Female; D019793:Fluorescein; D005456:Fluorescent Dyes; D006801:Humans; D008086:Lissamine Green Dyes; D008297:Male; D008537:Meibomian Glands / Q000502:physiology*; D008875:Middle Aged; D011446:Prospective Studies; D012395:Rose Bengal; D013194:Staining and Labeling / Q000379:methods*; D013666:Tears / Q000378:metabolism
D005456:Fluorescent Dyes; D008086:Lissamine Green Dyes; D012395:Rose Bengal; C024537:green 5; D019793:Fluorescein