A novel approach to DNA damage assessments: measurement of the thymine glycol lesion.
A different approach to the measurement of DNA damage has been developed based on the fact that many lesions can be excised from DNA in the form of modified dinucleoside monophosphates. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used in conjunction with isotopically labeled internal standards to quantify the lesion. The method has several advantages, including high sensitivity for the detection of dinucleoside monophosphates. The method was applied to the measurement of the 5,6-dihydroxy-5,6-dihydrothymine (thymine glycol) lesion in the DNA of mouse fibroblast cells exposed in culture to various treatments including ionizing radiation, UVC light and buthionine sulfoximine (BSO), an inhibitor of glutathione synthesis. The application of the method to the measurement of other DNA lesions is discussed.
Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
Douglas T
DT
Bailey
Han-Chun C
HC
DeFedericis
Kellee F
KF
Greene
Herbert
H
Iijima
Edwin E
EE
Budzinski
Helen B
HB
Patrzyc
Jean B
JB
Dawidzik
Harold C
HC
Box
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects*; D004249:DNA Damage; D015226:Dinucleoside Phosphates / Q000737:chemistry / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008809:Mice, Inbred C3H; D009152:Mutagenicity Tests / Q000379:methods*; D011829:Radiation Dosage; D013941:Thymine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000528:radiation effects
D015226:Dinucleoside Phosphates; C029389:thymine glycol; D004247:DNA; D013941:Thymine