[Berberine and amitozine binding with calf thymus DNA].
Binding of amitozine and berberine to DNA has been investigated by VIS- and UV-spectroscopy. It has been found that amitozine forms one type of complex and berberine forms two types of complexes with DNA. Observed concentration dependences of absorption spectra were analyzed using the DALSMOD optimization program and association constants were calculated (K(BCl)= 3 x 10(3) M(-1), K(Am) = 1.6 x 10(3)-10(4) M(-1)). Competitive binding of berberine to DNA in presence of ethidium bromide has been investigated as well. It has been shown that it competes with berberine for DNA binding sites.
E B
EB
Kruglova
E L
EL
Ermak
T P
TP
Soloshchuk
A I
AI
Potopal'skiĭ
Iu A
IuA
Potopal'skaia
D000478:Alkylation; D000818:Animals; D001599:Berberine / Q000737:chemistry; D001600:Berberine Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D001667:Binding, Competitive; D028281:Chelidonium / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D008024:Ligands; D015394:Molecular Structure; D028321:Plant Preparations / Q000737:chemistry / Q000302:isolation & purification; D013852:Thiotepa / Q000737:chemistry
D001600:Berberine Alkaloids; D008024:Ligands; D028321:Plant Preparations; D001599:Berberine; D004247:DNA; D013852:Thiotepa; C427203:calf thymus DNA