Capillary electrophoretic competitive immunoassay with laser-induced fluorescence detection for methionine-enkephalin.
Immunoassays are commonly used in bioresearch for the detection and quantification of small proteins and macromolecules in biological fluids and other complex matrices. In this report, a competitive immunoassay using capillary electrophoresis (CE) with laser-induced fluorescence was developed for methionine-enkephalin (ME). The method is based on the competitive reaction between the ME and fluorescein conjugated ME (ME-F) with anti-ME antibody, capillary electrophoresis separation of the ME-antibody bound and free ME-F, followed by the laser-induced fluorescence detection of the fluorescent species. With the optimized separation conditions, it was possible to separate the antibody bound and free fluorescien conjugated ME by a capillary electrophoresis-laser-induced fluorescence (CE-LIF) analysis using an uncoated fused-silica capillaries. The results concluded that the assay specificity, selectivity and accuracy were excellent.
Babu
C V Suresh
CV
Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Cheongryang, Seoul.
Chung
Bong Chul
BC
Lho
Dong Seok
DS
Yoo
Young Sook
YS
D019075:Electrophoresis, Capillary / Q000379:methods*; D004744:Enkephalin, Methionine / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D007834:Lasers; D009369:Neoplasms / Q000097:blood; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004744:Enkephalin, Methionine