Cytotoxic 5-aryl-1-(4-nitrophenyl)-3-oxo-1,4-pentadienes mounted on alicyclic scaffolds.
The 5-aryl-1-(4-nitrophenyl)-3-oxo-1,4-pentadienyl pharmacophore was incorporated into four series of compounds 1-4. Compounds 1a-g comprised a cluster of 3-arylidene-1-(4-nitrophenylmethylene)-2-oxo-3,4-dihydro-1H-naphthalenes while the analogues 2a-g consisted of a group of 6-arylidene-2-(4-nitrophenylmethylene)cyclohexanones. Three other compounds prepared in this study were 1-(4-nitrophenylmethylene)-3-(3,4,5-trimethoxyphenylmethylene)-2-oxo-2,3-dihydro-1H-indene 3a as well as two 5-arylidene-2-(4-nitrophenylmethylene)cyclopentanones 4a,b. The compounds were evaluated against human Molt 4/C8 and CEM T-lymphocytes as well as murine L1210 cells. In general, the compounds in series 1 displayed marked cytotoxicity having IC50 values in the 1-5 microM range while the related cyclohexyl analogues in series 2 were slightly less potent (IC50 figures were mainly 5-10 microM). The relative locations of two aryl rings present in all four series were considered to contribute significantly to bioactivity and may have accounted for the virtual absence of cytotoxic properties in series 3 and 4. Most of the compounds were administered intraperitoneally to mice using doses up to and including 300 mg/kg. No mortalities were noted. The inhibiting effect of most of the compounds towards Helicobacter pylori is noteworthy. The modes of action of representative compounds include the induction of apoptosis while some compounds weakly inhibited tubulin polymerisation and human N-myristoyltransferase.
College of Pharmacy and Nutrition, University of Saskatchewan, 110 Science Crescent, Saskatoon, Saskatchewan S7N 5C9, Canada.
Umashankar
U
Das
H Inci
HI
Gul
Jane
J
Alcorn
Anuraag
A
Shrivastav
Theresa
T
George
Rajendra K
RK
Sharma
Kurt H
KH
Nienaber
Erik
E
De Clercq
Jan
J
Balzarini
Masami
M
Kawase
Noriyuki
N
Kan
Toru
T
Tanaka
Satoru
S
Tani
Karl A
KA
Werbovetz
Adam J
AJ
Yakovich
Elias K
EK
Manavathu
James P
JP
Stables
Jonathan R
JR
Dimmock
D000466:Alkadienes / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003500:Cyclization; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D009596:Nitrophenols / Q000737:chemistry*; D013329:Structure-Activity Relationship
D000466:Alkadienes; D009596:Nitrophenols