Horseradish peroxidase-driven fluorescent labeling of nanotubes with quantum dots.
We describe the first enzyme-driven technique for fluorescent labeling of single-walled carbon nanotubes (SWNTs). The labeling was performed via enzymatic biotinylation of nanotubes in the tyramide-horseradish peroxidase (HRP) reaction. Both direct and indirect fuorescent labeling of SWNTs was achieved using either biotinyl tyramide or fluorescently tagged tyramides. Biotinylated SWNTs later reacted with streptavidin-conjugated fluorophores. Linking semiconductor nanocrystals, quantum dots (Q-dots), to the surface of nanotubes resulted in their fluorescent visualization, whereas conventional fluorophores bound to SWNTs directly or through biotin-streptavidin linkage, were completely quenched. Enzymatic biotinylation permits fluorescent visualization of carbon nanotubes, which could be useful for a number of biomedical applications. In addition, other organic molecules such as proteins, antibodies, or DNA can be conjugated to biotinylated SWNTs using this approach.
Baylor College of Medicine, Houston, TX, USA. vdidenko@bcm.tmc.edu
Vladimir V
VV
Didenko
David S
DS
Baskin
D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D045663:Quantum Dots; D013194:Staining and Labeling / Q000379:methods
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase