Characteristics of rabbit muscle adenylate kinase inhibition by ascorbate.
Earlier studies [1-3] showed that of the glycolytic enzymes, the muscle isozymes PFK-1, LDH, and AK were inhibited by ascorbic acid. These studies on the characteristics of the inhibition of RMAK by ascorbate are part of a hypothesis [3] that ascorbate facilitates the storage of skeletal muscle glycogen by inhibiting glycolysis when the muscle is at rest. These studies examine conditions for RMAK inhibition, prevention of inhibition, and reversal of ascorbate inhibition. We found that the concentration of RMAK was an important condition for inhibition. Above 200 nM RMAK, inhibition by ascorbate could not be demonstrated and below that concentration RMAK became increasingly sensitive to ascorbate inhibition. Associated with increased sensitivity to inhibition by ascorbate is a deviation from a linear to a concave relationship between low RMAK concentrations and enzyme activity. At low RMAK concentrations, the concave relationship becomes convex in the presence of muscle aldolase. In addition, aldolase reverses inhibitions by ascorbate. A comparison of inhibition of RMAK byascorbate and inhibition of LDH-m4 [3] is discussed. Other proteins prevent RMAK inhibition but do not reverse inhibition by ascorbate. The role of RMAK as a factor in the control of the rate of glycolysis is presented as is the role of compartmentalization with respect to the proposed role for ascorbate inhibition.
Department of Biology, University of California 0690, San Diego, La Jolla, CA 92093-0690, USA. prussell@ucsd.edu
Percy J
PJ
Russell
Anita
A
Williams
Ami
A
Abbott
Bibiane
B
DeRosales
Reynaldo
R
Vargas
D000263:Adenylate Kinase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D005634:Fructose-Bisphosphate Aldolase / Q000037:antagonists & inhibitors; D018482:Muscle, Skeletal / Q000201:enzymology*; D011817:Rabbits; D013379:Substrate Specificity
D000263:Adenylate Kinase; D005634:Fructose-Bisphosphate Aldolase; D001205:Ascorbic Acid