An antifungal protein from the pea Pisum sativum var. arvense Poir.
An antifungal protein with a molecular mass of 11 kDa and a lysine-rich N-terminal sequence was isolated from the seeds of the pea Pisum sativum var. arvense Poir. The antifungal protein was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel and CM-cellulose. It exerted antifungal activity against Physalospora piricola with an IC50 of 0.62 microM, and also antifungal activity against Fusarium oxysporum and Mycosphaerella arachidicola. It inhibited human immunodeficiency virus type 1 reverse transcriptase with an IC50 of 4.7 microM.
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China.
H X
HX
Wang
T B
TB
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D000596:Amino Acids / Q000737:chemistry; D000935:Antifungal Agents / Q000494:pharmacology*; D002474:Cell-Free System; D002482:Cellulose / Q000737:chemistry; D004983:Ethanolamines / Q000737:chemistry; D005670:Fusarium / Q000378:metabolism; D054303:HIV Reverse Transcriptase / Q000378:metabolism; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D018532:Pisum sativum / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*; D010936:Plant Extracts / Q000378:metabolism* / Q000494:pharmacology; D014176:Protein Biosynthesis; D014357:Trypsin / Q000494:pharmacology
D000596:Amino Acids; D000935:Antifungal Agents; D004983:Ethanolamines; D010455:Peptides; D010936:Plant Extracts; D002482:Cellulose; D054303:HIV Reverse Transcriptase; D014357:Trypsin; C007369:2-diethylaminoethanol