Genomic profiling identifies discrete deletions associated with translocations in glioblastoma multiforme.
Glioblastoma multiforme is the most common tumor arising in the central nervous system. Patients with these tumors have limited treatment options and their disease is invariably fatal. Molecularly targeted agents offer the potential to improve patient treatment, however the use of these will require a fuller understanding of the genetic changes in these complex tumors. In this study, we identify copy number changes in a series of glioblastoma multiforme tumors and cell lines by applying high-resolution microarray comparative genomic hybridization. Molecular cytogenetic characterization of the cell lines revealed that copy number changes define translocation breakpoints. We focused on chromosome 6 and further characterized three regions of copy number change associated with translocations including a discrete deletion involving IGF2R, PARK2, PACRG and QKI and an unbalanced translocation involving POLH, GTPBP2 and PTPRZ1.
Human Cytogenetics Laboratory, Cancer Research, UK.
Paul J
PJ
Mulholland
Heike
H
Fiegler
Chiara
C
Mazzanti
Patricia
P
Gorman
Peter
P
Sasieni
Joanna
J
Adams
Tania A
TA
Jones
Jane W
JW
Babbage
Radost
R
Vatcheva
Koichi
K
Ichimura
Philip
P
East
Chrysanthos
C
Poullikas
V Peter
VP
Collins
Nigel P
NP
Carter
Ian P M
IP
Tomlinson
Denise
D
Sheer
D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D002872:Chromosome Deletion; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D003582:Cytogenetics; D005260:Female; D018628:Gene Dosage / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D015894:Genome, Human / Q000235:genetics*; D023281:Genomics; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D014178:Translocation, Genetic / Q000235:genetics*