Transient incorporation of native GluR2-lacking AMPA receptors during hippocampal long-term potentiation.
Postnatal glutamatergic principal neuron synapses are typically presumed to express only calcium-impermeable (CI), GluR2-containing AMPARs under physiological conditions. Here, however, we demonstrate that long-term potentiation (LTP) in CA1 hippocampal pyramidal neurons causes rapid incorporation of GluR2-lacking calcium-permeable (CP)-AMPARs: CP-AMPARs are present transiently, being replaced by GluR2-containing AMPARs approximately 25 min after LTP induction. Thus, CP-AMPARs are physiologically expressed at CA1 pyramidal cell synapses during LTP, and may be required for LTP consolidation.
Plant
Karen
K
MRC Centre for Synaptic Plasticity, Department of Anatomy, University of Bristol, School of Medical Sciences, Bristol BS8 1TD, UK.
Pelkey
Kenneth A
KA
Bortolotto
Zuner A
ZA
Morita
Daiju
D
Terashima
Akira
A
McBain
Chris J
CJ
Collingridge
Graham L
GL
Isaac
John T R
JT
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D006624:Hippocampus / Q000166:cytology*; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D051379:Mice; D018733:Nicotinic Antagonists / Q000494:pharmacology; D018408:Patch-Clamp Techniques / Q000379:methods; D011073:Polyamines / Q000494:pharmacology; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D051381:Rats; D018091:Receptors, AMPA / Q000737:chemistry / Q000172:deficiency / Q000502:physiology*; D013997:Time Factors
D018733:Nicotinic Antagonists; D011073:Polyamines; D018091:Receptors, AMPA; C034092:delta-philanthotoxin; C104722:glutamate receptor ionotropic, AMPA 2; D002118:Calcium