Mapping of the habenulo-interpeduncular pathway in living mice using manganese-enhanced 3D MRI.
This magnetic resonance imaging (MRI) study describes mapping of the habenulo-interpeduncular pathway in living mice based on manganese-induced contrast. Six hours after intracerebroventricular microinjection of MnCl2, T1-weighted 3D MRI (2.35 T) at 117 mum isotropic resolution revealed a continuous pattern of anterograde labeling from the habenula via the fasciculus retroflexus to the interpeduncular nucleus. Alternatively, the less invasive systemic administration of MnCl2 allowed for monitoring of the dynamic uptake pattern of respective neural components with even higher reproducibility across animals. Time courses covered the range from 42 min to 24 h after injection. In conclusion, manganese-enhanced MRI may open new ways for functional assessments of the habenulo-interpeduncular system in animal models with cognitive impairment.
Biomedizinische NMR Forschungs GmbH am Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany. twatana@gwdg.de
Takashi
T
Watanabe
Jelena
J
Radulovic
Susann
S
Boretius
Jens
J
Frahm
Thomas
T
Michaelis
D000818:Animals; D001931:Brain Mapping; D002712:Chlorides / Q000008:administration & dosage / Q000494:pharmacology*; D003287:Contrast Media; D019262:Habenula / Q000033:anatomy & histology*; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D017895:Manganese Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections; D009434:Neural Pathways; D015203:Reproducibility of Results; D013997:Time Factors
D002712:Chlorides; D003287:Contrast Media; D017895:Manganese Compounds