[Analysis of LRP16 gene promoter activity].
The study was aimed to analyze the characteristics of LRP16 gene promoter and its activity in order to explore the possible regulation mechanism of LRP16 gene expression. A 2.6 kb genomic DNA sequence of LRP16 5'-end was obtained from NCBI by BLAST software. The 7 target sequences between 0.2 - 2.6 kb from a healthy blood donor DNA sample were amplified by PCR, then identified by DNA sequencing and semi-nest PCR. The verified sequences were analyzed on-line. The results showed that the 7 target sequences were about 400 bp different from each other. All 7 sequences were the same to these GenBank described. At last, all 7 promoter sequences were ligated with luciferase vector, and then the luciferase activity was analyzed in HeLa cells. A known gene promoter sequence can be freely obtained from NCBI database. It is concluded that LRP16 promoter is a standard type II promoter and its activity is strongest in the region from -200 to -600 bp.
Department of Gerontological Hematology, General Hospital of PLA, Beijing 100853, China.
Xue-Chun
XC
Lu
Fang-Ding
FD
Lou
Wei-Dong
WD
Han
Xu-Dong
XD
Zhu
Yim-Ming
YM
Mu
Zhou-Min
ZM
Xu
Li
L
Yu
D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases; D002880:Chromosomes, Human, Pair 11; D015870:Gene Expression; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
D009363:Neoplasm Proteins; D008156:Luciferases; D002265:Carboxylic Ester Hydrolases; C476832:MACROD1 protein, human