Physiological role of the putative ammonium transporter RhCG in the mouse.
Ammonium excretion into urine is a major process essential to the regulation of acid-base homeostasis. We have shown that Rh-type proteins, including renal RhCG, belong to the Mep/Amt family of ammonium transporters and promote bi-directional ammonium transport upon heterologous expression in yeast. To study the physiological role of RhCG and to test a potential function in ammonium excretion, we have generated mice bearing an invalidation of the corresponding gene.
Biver
S
S
Laboratoire de biologie du développement, institut de biologie et de médecine moléculaires, université libre de Bruxelles CP300, rue des professeurs Jeener-et-Brachet, 12, 6041 Gosselies, Belgium.
Scohy
S
S
Szpirer
J
J
Szpirer
C
C
André
B
B
Marini
A-M
AM
D000138:Acidosis / Q000378:metabolism; D000818:Animals; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism; D027682:Cation Transport Proteins / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D017136:Ion Transport; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D005810:Multigene Family; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D001798:Blood Proteins; D027682:Cation Transport Proteins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D000644:Quaternary Ammonium Compounds; C498523:RHAG protein, human; C413213:RHCG protein, human; D011993:Recombinant Fusion Proteins; C508143:Rh type C glycoprotein, rat; C486033:RhBG protein, mouse; C485880:Rhcg protein, mouse