Functional reconstitution of photosynthetic reaction centers in planar lipid bilayers.
Planar lipid bilayers containing reaction centers from Rhodopseudomonas sphaeroides R-26 were formed by apposing two reaction center-lipid monolayers formed from a reaction center-lipid complex in hexane. Secondary donors (cytochrome c) and acceptors (ubiquinone-0) were added on opposite sides of the membrane. Upon illumination, this system generated transient and steady-state voltages and currents. The wavelength dependence of the photoresponse matched the absorption spectrum of reaction centers. A simple model based on the transfer of charges across the membrane that explains the salient features of the photoresponse is presented.
Schönfeld
M
M
Department of Biology, University of California at San Diego, La Jolla, California 92093.
Montal
M
M
Feher
G
G