Hydrogen peroxide induces vascular permeability via regulation of vascular endothelial growth factor.
Oxidative stress plays critical roles in initiation and/or worsening of respiratory disease process. Although reactive oxygen species (ROS) are shown to cause vascular leakage, the mechanisms by which ROS induce an increase in vascular permeability are not clearly understood. In this study, we have used a murine model to evaluate the effect of hydrogen peroxide (H(2)O(2)) to examine roles of ROS and the molecular mechanism in vascular permeability. The results have revealed that ROS levels, vascular endothelial growth factor (VEGF) expression, hypoxia-inducible factor-1alpha protein level, airway hyperresponsiveness, and vascular permeability are increased after inhalation of H(2)O(2). Administration of antioxidants markedly reduced plasma extravasation and VEGF levels in lungs treated with H(2)O(2). These results indicate that ROS may modulate vascular permeability via upregulation of VEGF expression.
Department of Internal Medicine, Chonbuk National University Medical School, San 2-20 Geumam-dong, Jeonju, Jeonbuk 561-180, South Korea.
Kyung Sun
KS
Lee
So Ri
SR
Kim
Seoung Ju
SJ
Park
Hee Sun
HS
Park
Kyung Hoon
KH
Min
Min Hee
MH
Lee
Sun Mi
SM
Jin
Gong Yong
GY
Jin
Wan Hee
WH
Yoo
Yong Chul
YC
Lee
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D016877:Oxidants / Q000494:pharmacology*; D013047:Specific Pathogen-Free Organisms; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000502:physiology
D016877:Oxidants; D042461:Vascular Endothelial Growth Factor A; D006861:Hydrogen Peroxide