[Molecular cloning of tomato LeHsp110/ClpB gene and its effect on the thermotolerance in plant].
The heat shock protein ClpB is a member of the Clp family and functions as molecular chaperones. ClpB is related to the acquired thermotolerance in organisms. A cDNA of 3144 bp was screened out of a tomato cDNA library. The polypeptide deduced from the longest ORF contains 980 amino acid residues, and was classified into HSP100/ClpB family based on the result of molecular phylogenesis analysis. Thus it was named as LeHSP110/ClpB according to its calculated molecular weight. LeHSP110/ClpB was characteristic of heat-inducibility but no constitutive expression, and was demonstrated to locate in chloroplastic stroma. An antisense cDNA fragment of LeHsp110/ClpB under the control of CaMV 35S promoter was introduced into tomato by Agrobacterium tumefactions-mediated method. At high temperature, the mRNA levels of LeHsp110/ClpB in antisense transgenic plants were lower than those in control plants. The PS II of transgenic plants is more sensitive to high temperature than that of control plants according to data of Fv/Fm. These results clearly showed that HSP110/ClpB plays an important role in thermotolerance of high plants.
Yang
Jin-Ying
JY
College of Life Science of Shandong Normal University, Jinan 250014, China.
Sun
Ying
Y
Sun
Ai-Qing
AQ
Yi
Shu-Ying
SY
Liu
Jian
J
D000222:Adaptation, Physiological / Q000235:genetics*; D002736:Chloroplasts / Q000378:metabolism; D003001:Cloning, Molecular; D017343:Genes, Plant / Q000235:genetics; D050957:HSP110 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D018551:Solanum lycopersicum / Q000235:genetics* / Q000502:physiology; D045332:Photosystem II Protein Complex / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology
D050957:HSP110 Heat-Shock Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins