[Reconstruction of urothelium tissue using issue-engineering technique].
OBJECTIVE
To evaluate the feasibility of reconstruction of urothelium tissue in vivo using tissue-engineering technique.


METHODS
The urothelium cells were obtained from young rabbit, bladder by mechanical and enzyme digested methods. After expanded in vitro, the 4th to 5th generation urothelium cells were seeded onto the surface of 8 Polylatical/glycolic acid copolymer polymer, the polymer matrix without seeding cells served as control group. A total of 8 cell-polymer scaffolds and 4 simply scaffolds were separately implanted into subcutaneous pockets of athymic mice. The experiment groups included cell-polymer scaffolds 4 weeks and cell-polymer scaffolds 8 weeks. The control group included simply scaffold 4 weeks and simply scaffold 8 weeks. After 4 and 8 weeks, the specimens were obtained and examined by gross inspection, histologically and immunohistochemically.


RESULTS
The results of HE and Masson staining showed that the polymer were covered by urothelium cells layers and cells layers increased markley in experimental group. Immunocytochemical studies revealed that the cells were stained positively for anti-cytokeratins (AE1/AE3) in experimental group. Fiber tissue deposition were found on the surface of polymers in control group by HE and Masson staining. Immunocytochemical staining of implants showed the negative result for cytokeratins in control group.


CONCLUSIONS
It is feasibility that reconstruction of urothelium tissue using tissue-engineering technique,which provides basic understandings for further development of the bladder and ureteral tissue engineered research.
Kidney Center of PLA, Departement of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P R China.
Weiping
W
Hou
Fahuan
F
Yuan
Changyong
C
Wang
Ximin
X
Guo
Benlan
B
Ye
Qiang
Q
Zhao
D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D005260:Female; D051379:Mice; D008819:Mice, Nude; D011100:Polyglycolic Acid; D011817:Rabbits; D019651:Plastic Surgery Procedures; D023822:Tissue Engineering / Q000379:methods*; D001743:Urinary Bladder / Q000166:cytology*; D019459:Urothelium
D001672:Biocompatible Materials; D011100:Polyglycolic Acid