Diltiazem, a L-type calcium channel antagonist, suppresses ouabain-enhanced dopamine efflux by 1-methyl-4-phenylpyridinium ion (MPP+) in rat striatum.
The present study was examined whether diltiazem, a L-type Ca2+ channel antagonist, could suppresses 1 methyl-4-phenylpyridinium ion (MPP+)-induced dopamine (DA) in extracellular fluid of rat striatum. Ouabain (100 microM; 100 microM or 100 pmol/microl per min) significantly enhanced the level of DA by MPP+. However, in the presence of diltiazem (100 microM) significantly suppressed the level of DA release by ouabain and MPP+. These results suggest that diltiazem suppresses Ca2+ -dependent release of DA by ouabain-induced Ca2+ overload.
Department of Analytical Chemistry, School of Pharmaceutical Sciences, Ohu University, Koriyama, Fukushima 963-8611, Japan. t-obata@pha.ohu-u.ac.jp
Toshio
T
Obata
D015655:1-Methyl-4-phenylpyridinium / Q000037:antagonists & inhibitors*; D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004110:Diltiazem / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism*; D008297:Male; D010042:Ouabain / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar
D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D010042:Ouabain; D004110:Diltiazem; D015655:1-Methyl-4-phenylpyridinium; D002118:Calcium; D004298:Dopamine