Development of recombinant chicken IgY from single chain fragment of variable region for diagnosis of BSE.
We generated two recombinant chicken IgYs, designated Ab3-15 and Ab4-19, against mammalian prion protein (PrP) from the single chain fragment of variable region (scFv) antibodies. These two antibodies recognized PrP(Sc) from bovine spongiform encephalopathy (BSE) in cattle and were more sensitive than the corresponding scFv antibodies. These antibodies also recognized PrP(Sc) from other scrapie-infected mammals. These results indicate that Ab3-15 and Ab4-19 are useful for diagnosis of BSE as well as other prion diseases.
Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan.
Kazuyoshi
K
Miyamoto
Toshi
T
Shimamoto
Masayoshi
M
Aosasa
Sota
S
Kimura
Naoto
N
Nakamura
Yuka
Y
Okubo
Takashi
T
Yokoyama
Hiroyuki
H
Horiuchi
Shuichi
S
Furusawa
Haruo
H
Matsuda
D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D002645:Chickens / Q000276:immunology*; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D007136:Immunoglobulins / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification*
C016512:IgY; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; D011994:Recombinant Proteins