Coconut water as a medium additive for the production of docosahexaenoic acid (C22:6 n3) by Schizochytrium mangrovei Sk-02.
The effect of coconut water (CW) on biomass and docosahexaenoic acid (DHA, C22:6 n3) formation by Schizochytrium mangrovei Sk-02 was studied in a yeast extract-diluted sea water medium. Optimal CW-level was ca. 33% (v/v), resulting in a biomass level of 28 g/l with a DHA-content of 20% (w/w) or 6 g DHA/l, almost 50% higher than in non-supplemented cultures at the same initial sugar level. Study on the growth-promoting effects of coconut water suggested that it could be (partially) mimicked by addition of trace elements; the fatty acids present in CW did not appear to be incorporated or effect fatty acid formation by the organism. CW-addition was also effective in media with other nitrogen sources such as casitone, peptone and tryptone. Its inclusion (at 50% v/v) increased biomass levels two-to-three-fold with concomitant increases in the DHA-level.
Unagul
Panida
P
BIOTEC, 113 Thailand Science Park, Phaholyothin Rd, Klong 1, Klong Luang, Pathum Thani 12120, Thailand.
Assantachai
Caetharin
C
Phadungruengluij
Saranya
S
Suphantharika
Manop
M
Tanticharoen
Morakot
M
Verduyn
Cornelis
C
D000818:Animals; D019149:Bioreactors; D003059:Cocos / Q000737:chemistry*; D003470:Culture Media; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D056890:Eukaryota / Q000378:metabolism*; D014867:Water / Q000737:chemistry*
D003470:Culture Media; D014867:Water; D004281:Docosahexaenoic Acids