Optical waveguide biosensors constructed with subwavelength gratings.
The reflection resonance spectrum of a subwavelength diffraction-grating-coupled waveguide is used to analyze biomolecular interactions in real time. By detecting this resonance wavelength shift, the optical waveguide biosensor provides the ability to identify the kinetics of the biomolecular interaction on an on-line basis without the need for extrinsic labeling of the biomolecules. A theoretical analysis of the subwavelength optical waveguide biosensor is performed. A biosensor with a narrow reflection resonance spectrum, and hence an enhanced detection resolution, is then designed and fabricated. Currently, the detection limit of the optical waveguide sensor is approximately 10(-5) refractive-index units. The biosensor is successfully applied to study of the dynamic response of an antibody interaction with protein G adsorbed on the sensing surface.
Yih
Jenq-Nan
JN
Institute of Optical Sciences, National Central University, Chung-Li 320, Taiwan.
Chu
Yi-Ming
YM
Mao
Yen-Chieh
YC
Wang
Wei-Han
WH
Chien
Fan-Ching
FC
Lin
Chun-Yu
CY
Lee
Kuang-Li
KL
Wei
Pei-Kuen
PK
Chen
Shean-Jen
SJ
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D055095:Optics and Photonics / Q000295:instrumentation*; D025941:Protein Interaction Mapping / Q000295:instrumentation* / Q000379:methods; D012031:Refractometry / Q000295:instrumentation* / Q000379:methods
C029152:G-substrate; D009419:Nerve Tissue Proteins