The role of interleukin-17 during acute rejection after lung transplantation.
Acute rejection (AR) is an important complication that can occur after lung transplantation and constitutes a risk factor for bronchiolitis obliterans syndrome, which is characterised by a neutrophilic airway inflammation. The specific aim of this study was to investigate the role of interleukin (IL)-17, which promotes chemotaxis of neutrophils by inducing IL-8 production, in AR. Cell differentials, mRNA and protein levels were quantified in bronchoalveolar lavages (BALs) taken from patients at 28 and 90 days after lung transplantation. The patient's rejection status was assessed by transbronchial biopsy. An AR was found in nine out of the 26 patients examined, 28 days after transplantation. The number of BAL neutrophils and lymphocytes were increased in these patients. IL-17 mRNA and protein levels in the BAL were increased in patients with AR. Analysis of BAL obtained at day 90 after transplantation, demonstrated that the increase in IL-17 had disappeared, whereas the increase in neutrophils and lymphocytes persisted. These data showed that interleukin-17 is temporarily upregulated in bronchoalveolar lavage during acute rejection. The number of lymphocytes and neutrophils are increased in bronchoalveolar lavage during acute rejection and may persist up to 2 months after acute rejection. These findings suggest that interleukin-17 is important in the pathophysiology of acute lung rejection.
Vanaudenaerde
B M
BM
Laboratory of Pneumology, Katholieke Universiteit, and Lung Transplantation Unit, University Hospital Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium.
Dupont
L J
LJ
Wuyts
W A
WA
Verbeken
E K
EK
Meyts
I
I
Bullens
D M
DM
Dilissen
E
E
Luyts
L
L
Van Raemdonck
D E
DE
Verleden
G M
GM
D000208:Acute Disease; D000328:Adult; D001706:Biopsy; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D001999:Bronchoscopy; D002634:Chemotaxis, Leukocyte; D005260:Female; D015870:Gene Expression; D006084:Graft Rejection / Q000276:immunology* / Q000473:pathology; D006801:Humans; D020381:Interleukin-17 / Q000097:blood* / Q000235:genetics; D016209:Interleukin-8 / Q000097:blood / Q000235:genetics; D007958:Leukocyte Count; D008168:Lung / Q000473:pathology; D016040:Lung Transplantation / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics
D020381:Interleukin-17; D016209:Interleukin-8; D012333:RNA, Messenger