[Determination of epicatechin in Rhizoma Fagopyri dibotoryis by HPLC-ECD].
OBJECTIVE
To establish a quantitative method of determination of epicatechin in Rhizoma fagopyri dibotoryis by HPLC-electrochemical detection.


METHODS
The sample was separated on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol-0.1 mol x L(-1) phosphate buffer (1:3, pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the applied potential was + 600 mV.


RESULTS
The linear range of epicatechin was 0.004 875-1.56 microg, r = 0.999 9. The average recovery was 100.7% with RSD 1.9% (n = 5). The content of epicatechin was different in nine samples purchased.


CONCLUSIONS
The method is good reproducible and can be used to determine epicatechin in Rhizoma fagopyri dibotoryis.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Min-Tong
MT
Xin
Bin
B
Yang
Hua
H
Li
Lu-Qi
LQ
Huang
D002392:Catechin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D019612:Fagopyrum / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry
D002392:Catechin