Sequence analysis and receptor specificity of the hemagglutinin of a recent influenza H2N2 virus isolated from chicken in North America.
Influenza viruses bind host cells following an interaction between the viral hemagglutinin (HA) protein and host cell sialylated glycoproteins and glycolipids. Differences in binding affinities of the HAs for different types of sialic acid linkages (alpha2-3 vs. alpha2-6) contribute to determining the host range of an influenza virus. The ability of an avian influenza virus HA to bind the human form of the receptor may be one requirement for an avian virus to propagate in the human population. In this paper, we describe the characterization of the HA from an H2N2 virus isolated from a Pennsylvania chicken farm in 2004. Sequence analysis revealed that this HA is a member of the Eurasian clade, and receptor binding studies show that it maintains its specificity for the avian influenza virus alpha2-3 linked sialic acid receptor.
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA.
Laurel
L
Glaser
Dmitriy
D
Zamarin
Helen M
HM
Acland
Erica
E
Spackman
Peter
P
Palese
Adolfo
A
García-Sastre
Deepanker
D
Tewari
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000821:virology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry* / Q000378:metabolism*; D053121:Influenza A Virus, H2N2 Subtype / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D010414:Pennsylvania; D010802:Phylogeny; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C092488:sialic acid receptor