Expression pattern of alphavbeta3 and alphavbeta5 integrin mRNA in mouse fetal gonads.
The alphavbeta3 and alphavbeta5 integrins are known as transmembrane receptors capable of binding to the RGD amino acid peptide sequence. In mouse early gonadogenesis, some proteins containing the RGD sequence are deposited into extracellular space and participate in morphogenesis. We analyzed the expression patterns of the alphavbeta3 and alphavbeta5 integrins in mouse developing gonads (10.5-13.5 days post coitum) using whole-mount in situ hybridization. The alphav integrin mRNA was homogenously expressed in developing gonadal regions. On the other hand, the beta3 integrin mRNA was found only in large and round cells (presumptive germ cells), whereas beta5 integrin was localized in gonadal somatic cells, with the exception of coelomic epithelial cells. The beta3 integrin-expressed cells were determined to be primordial germ cells because the number of these cells was drastically reduced in busulfan-treated gonads. In this study, we demonstrated that the alphavbeta3 and alphavbeta5 integrins are widely localized in the mouse developing gonads and discussed their presumptive functions on mouse gonadogenesis.
Ishii
Maki
M
Department of Veterinary Anatomy, The University of Tokyo, Japan. ishiima@tmin.ac.jp
Tay
Tat Wei
TW
Matsui
Toshiyasu
T
Kidokoro
Tomohide
T
Mizukami
Takuo
T
Kanai
Yoshiakira
Y
Hayashi
Yoshihiro
Y
Kurohmaru
Masamichi
M
D000818:Animals; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D006066:Gonads / Q000737:chemistry / Q000166:cytology / Q000196:embryology*; D039302:Integrin alphaVbeta3 / Q000235:genetics*; D016023:Integrins / Q000235:genetics*; D051379:Mice; D009024:Morphogenesis / Q000235:genetics; D009842:Oligopeptides; D012333:RNA, Messenger / Q000032:analysis; D019030:Receptors, Vitronectin / Q000235:genetics*; D014018:Tissue Distribution
D039302:Integrin alphaVbeta3; D016023:Integrins; D009842:Oligopeptides; D012333:RNA, Messenger; D019030:Receptors, Vitronectin; C064660:integrin alphaVbeta5; C047981:arginyl-glycyl-aspartic acid