Determination of enzymatic activity by capillary electrophoresis.
Enzymes are biological catalysts that play an important role in biochemical reactions necessary for normal growth, maturation and reproduction through whole live world. Their accurate quantitation in biological samples is important in many fields of biochemistry, not only in routine biochemistry and in fundamental research, but also in clinical and pharmacological research and diagnosis. Since the direct measurement of enzymes by masses is impossible, they must be quantified by their catalytic activities. Many different methods have been applied for this purpose so far. Although photometric methods are undoubtedly the most frequently used, separation methods will further gain their position in this field. The article reviews different possibilities for the assay of enzymatic activity by means of capillary electrophoresis (CE). Both the off-line and on-line enzyme assays based on CE are discussed.
Glatz
Zdenek
Z
Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic. glatz@chemi.muni.cz
D002268:Carboxypeptidases / Q000032:analysis / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004798:Enzymes / Q000032:analysis* / Q000378:metabolism; D001616:beta-Galactosidase / Q000032:analysis / Q000378:metabolism
D004798:Enzymes; D001616:beta-Galactosidase; D002268:Carboxypeptidases