Loss of Ku70/Ku80 expression occurs more frequently in hereditary than in sporadic colorectal tumors. Tissue microarray study.
Ku70 and Ku80 proteins take part in the repairing of DNA double-strand breaks by their function as a regulatory subunit of the DNA-dependent protein kinase. In this study, expression of both genes was analyzed in colorectal carcinoma tissue arrays applying immunohistochemistry. Expression of both genes decreased along with pT stage. Significant differences in Ku70 and Ku80 expression were found between pT3 and pT4 as well as between pT2 and pT3 tumors, respectively. Loss of Ku70/Ku80 expression was more frequently observed in hereditary than in sporadic tumors. We conclude that expression of Ku70/Ku80 genes is down-regulated in colorectal carcinoma and that defects of these genes are more frequently observed in hereditary than in sporadic tumors.
Department of Pathology, Reinhard Nieter Hospital-Wilhelmshaven, Academic Hospital of the University Göttingen, Germany. korabmon@yahoo.de
Monika
M
Korabiowska
Carlos
C
Cordon-Cardo
Michael
M
Schinagl
Michael
M
Karaus
Jerzy
J
Stachura
Harald
H
Schulz
Gösta
G
Fischer
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D034961:Antigens, Nuclear / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D015331:Cohort Studies; D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000378:metabolism* / Q000473:pathology; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D000072200:Ku Autoantigen; D008297:Male; D008875:Middle Aged; D046888:Tissue Array Analysis / Q000379:methods*
D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen