Molecular analysis of the magnocellular neuroendocrine phenotype: from the micropunch to laser microdissection.
Microdissection of selected regions of central nervous system (CNS) has provided the basis of modern chemoarchitectonics. Laser microdissection is a modern variant of the "Palkovits punch" technique and used together with gene array analysis has revolutionalized CNS molecular analysis. Here we describe the use of such an approach to elucidate molecules selectively expressed in magnocellular neuroendocrine cells (MCNs) in the supraoptic nucleus (SON). We found 123 genes that are preferentially expressed in the SON, and of these, 89 were substantially osmoregulated in their expression. One of these, C1q domain containing 1, is a novel gene that is osmoregulated much more than even vasopressin itself.
Molecular Neuroscience Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
Noriko
N
Mutsuga
Harold
H
Gainer
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004210:Dissection / Q000379:methods*; D020869:Gene Expression Profiling; D007834:Lasers; D008297:Male; D009490:Neurosecretory Systems / Q000166:cytology* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley
D017931:DNA Primers