Rat brain proteome in morphine dependence.
The aim of this study was to reveal potential markers associated with drug dependence, using the proteomic approach. Gels containing samples derived from morphine-treated and control animals were compared and analyzed. Inspection of protein profiles, following TCA/acetone precipitation and the use of nano-scale liquid chromatography coupled to tandem mass spectrometry, allowed for identification of eleven potential dependence markers, mainly cytoplasmic and mitochondrial enzymes, e.g. proteins that belong to GTPase and GST superfamilies, ATPase, asparaginase or proteasome subunit p27 families.
Bierczynska-Krzysik
Anna
A
Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Krakow, Poland.
Bonar
Emilia
E
Drabik
Anna
A
Noga
Marek
M
Suder
Piotr
P
Dylag
Tomasz
T
Dubin
Adam
A
Kotlinska
Jolanta
J
Silberring
Jerzy
J
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D013058:Mass Spectrometry; D009021:Morphine Dependence / Q000378:metabolism*; D036103:Nanotechnology; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D020543:Proteome