[Serum levels of IL-1beta, IL-6 and TNF-alpha in infertile patients with ovarian dysfunction].
BACKGROUND
Some cytokines that take part in the ovarian cycle regulation, such as IL-1beta, IL-6 and TNF-alpha play an important role during growth and development of ovarian follicle. Quantification of some cytokines is promissory in the work-up of ovarian reproductive pathology.


OBJECTIVE
To determine serum levels of IL-1beta, IL-6 and TNF-alpha in infertile patients with ovarian dysfunction and to compare them with those found in ovulatory patients.


METHODS
We conducted a cross-sectional study in infertile patients with premature ovarian failure, diminished ovarian reserve, chronic anovulation and ovulatory patients that were well-characterized by clinical and hormonal parameters. We determined serum concentrations of IL-1beta, IL-6 and TNF-alpha by an ELISA method. Statistics was conducted by using one way ANOVA, Kruskall-Wallis and Pearson correlation analyses.


RESULTS
We studied a total of forty patients. The mean age was 30 years; we did not find significant differences among groups. Overall mean of cytokines concentration was: 13.2 pg/mL for IL-1beta; 8.4 pg/mL for IL-6; and 1.5 pg/mL for TNF-alpha. We observed a significant difference in the concentration of TNF-alpha in the premature ovarian failure group when it was compared to the control group. Pearson correlation coefficients were no significant.


CONCLUSIONS
A wide range of dispersion of serum cytokines concentration was observed. Serum concentrations of TNF-alpha in woman with premature ovarian failure were significantly lower than those in the control group. Our findings represent a precedent in the powerful utility of the quantification of these cytokines in reproductive medicine.
Vital Reyes
Victor Saúl
VS
Departamento de Biología de la Reproducción y Ginecoendocrinología, Hospital de Ginecología y Obstetricia, Centro Médico Nacional La Raza, Instituto Mexicano del Seguro Social. vitalito23@hotmail.com
Téllez Velasco
Sergio
S
Hinojosa Cruz
Juan Carlos
JC
Ortiz Romero
María de Jesús
Mde J
Chavarría Olarte
María Eugenia
ME
Reyes Fuentes
Alejandro
A
D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000097:blood* / Q000209:etiology; D007375:Interleukin-1 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D010049:Ovarian Diseases / Q000097:blood* / Q000150:complications; D010053:Ovary / Q000503:physiopathology*; D016649:Primary Ovarian Insufficiency / Q000097:blood / Q000150:complications; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha