Improving the initial biocompatibility of a titanium surface using an Er,Cr:YSGG laser-powered hydrokinetic system.
OBJECTIVE
This study was designed to improve the initial biocompatibility of titanium (Ti) using an Er,Cr:YSGG-powered hydrokinetic system.


METHODS
The Er,Cr:YSGG-powered hydrokinetic system with different laser energy densities, 125 and 190 J/cm(2), were applied to the Ti substrate. Human osteosarcoma U2-OS cells were used. The difference in the attached cell number between 3- and 1-days cell culture was calculated and defined as the initial cell proliferation index (CPI). The initial CPI was statistically analyzed using one-way analysis of variance with the factor of applied laser energy density. The surface spreading morphology of the attached cells after 1 day incubation was observed using a field-emission scanning electron microscope.


RESULTS
The Er,Cr:YSGG laser-treated Ti had a higher (1.2-1.3 times) initial CPI (P<0.001) and better cell spreading morphology than the untreated Ti. Treating the Ti with higher Er,Cr:YSGG laser energy did not significantly improve the CPI and cell spreading morphology.


CONCLUSIONS
The initial biocompatibility of the Ti surface could be improved using an Er,Cr:YSGG laser-powered hydrokinetic system.
Huang
Her-Hsiung
HH
Faculty of Dentistry, School of Dentistry, National Yang-Ming University, Taipei 112, Taiwan.
Chuang
Yu-Chiao
YC
Chen
Zhi-Hwa
ZH
Lee
Tien-Ling
TL
Chen
Chun-Cheng
CC
D000704:Analysis of Variance; D001672:Biocompatible Materials; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002857:Chromium; D045662:Dental Etching / Q000295:instrumentation*; D004871:Erbium; D006801:Humans; D007834:Lasers; D010006:Osteoblasts / Q000502:physiology; D013499:Surface Properties; D014025:Titanium; D014867:Water
D001672:Biocompatible Materials; D014867:Water; D002857:Chromium; D004871:Erbium; D014025:Titanium