Probing the microenvironments in the grooves of Z-DNA using dan-modified oligonucleotides.
The environment-sensitive fluorophore dan (6-dimethylamino-2-acyl-naphthalene)- modified dC or dG bases were introduced into the Z-DNA forming sequence. It was demonstrated that both grooves of Z-DNA are more hydrated than those of B-DNA. Dan will be useful for probing the microenvironments in the grooves among the DNA polymorphs.
Institute of Scientific and Industrial Research (SANKEN), Osaka University Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
Takumi
T
Kimura
Kiyohiko
K
Kawai
Tetsuro
T
Majima
D015081:2-Naphthylamine / Q000031:analogs & derivatives; D004247:DNA / Q000737:chemistry; D043542:DNA, Z-Form / Q000737:chemistry*; D005456:Fluorescent Dyes; D015336:Molecular Probe Techniques; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D043542:DNA, Z-Form; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D014867:Water; D004247:DNA; D015081:2-Naphthylamine