Fish oil regulates adiponectin secretion by a peroxisome proliferator-activated receptor-gamma-dependent mechanism in mice.
Adiponectin has insulin-sensitizing, antiatherogenic, and anti-inflammatory properties, but little is known about factors that regulate its secretion. To examine the effect of fish oil on adiponectin secretion, mice were fed either a control diet or isocaloric diets containing 27% safflower oil or 27, 13.5, and 8% menhaden fish oil. Within 15 days, fish oil feeding raised plasma adiponectin concentrations two- to threefold in a dose-dependent manner, and the concentrations remained approximately twofold higher for 7 days when the fish oil diet was replaced by the safflower oil diet. Within 24 h, fish oil markedly induced transcription of the adiponectin gene in epididymal adipose tissue but not in subcutaneous fat. The increase of plasma adiponectin by fish oil was completely blocked by administration of the peroxisome proliferator-activated receptor (PPAR)gamma inhibitor bisphenol-A-diglycidyl ether. In contrast, there was no effect of fish oil feeding on adiponectin secretion in PPARalpha-null mice. These data suggest that fish oil is a naturally occurring potent regulator of adiponectin secretion in vivo and that it does so through a PPARgamma-dependent and PPARalpha-independent manner in epididymal fat.
Neschen
Susanne
S
Howard Hughes Medical Institute, Yale Medical School, P.O. Box 9812, New Haven, CT 06536-8012, USA.
Morino
Katsutaro
K
Rossbacher
Jörg C
JC
Pongratz
Rebecca L
RL
Cline
Gary W
GW
Sono
Saki
S
Gillum
Matthew
M
Shulman
Gerald I
GI
D052242:Adiponectin / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001559:Benzhydryl Compounds; D018955:CD36 Antigens / Q000235:genetics; D017931:DNA Primers; D004822:Epididymis; D004852:Epoxy Compounds / Q000494:pharmacology; D005395:Fish Oils / Q000494:pharmacology*; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D047495:PPAR gamma / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects; D000098826:Bisphenol A Compounds
D052242:Adiponectin; D001559:Benzhydryl Compounds; D018955:CD36 Antigens; D017931:DNA Primers; D004852:Epoxy Compounds; D005395:Fish Oils; D047495:PPAR gamma; C019273:bisphenol A diglycidyl ether; D000098826:Bisphenol A Compounds