The effect of buthionine sulfoximine on the glutathione level in goldfish tissues.
This study describes the effect of DL-buthionine-[S,R]-sulfoximine (BSO) on the glutathione equivalents (GSH-eq = GSH + 2 GSSG) of goldfish. BSO causes depletion of cellular GSH by inhibiting gamma-glutamylcysteine synthetase, a key enzyme of the GSH biosynthesis pathway. BSO at 1,000 and 1,500 mg/kg was effective in promoting 50 and 80% depletion of GSH-eq from brain and liver, respectively, within 3 days. Lower doses of BSO failed to effectively promote hepatic GSH-eq depletion. Moreover, no evident toxic side-effects were observed (including hepatic lipid peroxidation and free radical-mediated oxidation of proteins) in goldfish in response to BSO intraperitoneal injections. We conclude that BSO can be used to deplete GSH-eq in goldfish liver and brain, but attention should be paid to species-specific variations in BSO effects.
Lushchak
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Vassyl Stefanyk Precarpathian University, Ivano-Frankivsk, Ukraine. lushchak@pu.if.ua
Hermes-Lima
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D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D019328:Buthionine Sulfoximine / Q000494:pharmacology*; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism*; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male
D019328:Buthionine Sulfoximine; D005978:Glutathione