Purification and characterization of prodigiosin produced by integrated bioreactor from Serratia sp. KH-95.
To date, prodigiosin and its analogues which have been shown to have anticancer, cytotoxic and immunosuppressive activities have been isolated from Serratia, Pseudomonas and Streptomyces species, and chemically synthesized. In a previous study, the red pigment content in Serratia sp. KH-95 was enhanced using a casein-enriched medium. Recently, an integrated bioreactor with an internal adsorbent has been developed to increase the production yield and allow easy recovery of the pigment. Thus, this study focused on both purifying and identifying a single red pigment from several pigments attached to the adsorbent in an integrated bioreactor. The red pigment was extracted directly from the internal adsorbent using acidified methanol and phase separation. Subsequently, it was purified by silica gel chromatography and high performance liquid chromatograph (HPLC). As a result, pure prodigiosin was identified by structural studies as a pigment. Also, this downstream procedure that uses the integrated bioreactor can be applied to the direct production and purification of other prodigiosin analogues and hydrophobic alkaloid compounds from several microorganisms.
Song
Min-Jung
MJ
Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea.
Bae
Jungdon
J
Lee
Dae-Sil
DS
Kim
Chang-Ho
CH
Kim
Jun-Seok
JS
Kim
Seung-Wook
SW
Hong
Suk-In
SI
D019149:Bioreactors; D002364:Caseins / Q000737:chemistry / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D011353:Prodigiosin / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D012705:Serratia / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
D002364:Caseins; D011353:Prodigiosin; D000432:Methanol