Identification of proteins in excretory/secretory extracts of Echinostoma friedi (Trematoda) from chronic and acute infections.
In the present study, we describe the investigation of Echinostoma friedi excretory/secretory products using a proteomic approach combined with the use of heterologous antibodies. We have identified 18 protein spots corresponding to ten proteins, including cytoskeletal proteins like actin, tropomyosin, and paramyosin; glycolytic enzymes like enolase, glyceraldehyde 3P dehydrogenase, and aldolase; detoxifying enzymes like GSTs; and stress proteins like heat shock protein (Hsp) 70. Among these proteins, both actin and, to a lesser extent, Hsp70, exhibited differential expression patterns between chronic and acute infections in the Echinostoma-rodent model, suggesting that these proteins may play a role in the survival within the host.
Departament de Bioquímica i Biologia Molecular, Facultat de Ciències, Universitat de Valencia, Burjassot, Valencia, Spain.
Dolores
D
Bernal
Inés
I
Carpena
Ana Maria
AM
Espert
José Enrique
JE
De la Rubia
José Guillermo
JG
Esteban
Rafael
R
Toledo
Antonio
A
Marcilla
D000208:Acute Disease; D000595:Amino Acid Sequence; D000818:Animals; D002908:Chronic Disease; D006224:Cricetinae; D004195:Disease Models, Animal; D004449:Echinostoma / Q000737:chemistry* / Q000378:metabolism*; D004451:Echinostomiasis / Q000235:genetics / Q000378:metabolism*; D008647:Mesocricetus; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity
D011506:Proteins