CF mediated G1 arrest is associated with induction of p27(Kip1) and inhibition of cyclin D1 expression in human hepatoma HepG2 cells.
CF is a kind of diterpenoid which was first isolated and purified from Chinese tropical plants by our laboratory. Our previous works have demonstrated it could inhibit the proliferation of several malignant tumor cell lines and stimulate them to differentiate to normal cells. In this article we investigated the effect of CF on human hepatocellulor carcinoma HepG2 cell viability, differentiation, cell cycle distribution and G1 cell cycle related genes expression. We also detected the effect of retinoic acid (RA) which was used as positive control and the effect of combination CF+RA. Our data suggested that CF could be useful to induce growth arrest and differentiation in HepG2 cell lines, and could reverse the transformed phenotype. This anti-tumor effect was due to G1 arrest in cell cycle which was associated with an increase of p27(Kip1) and a decrease of cyclin D1 expression, so CF might be a useful targeted therapy strategy for HCC. Results also showed RA has a different mechanism from CF on G1 arrest, and CF has not synergistic anti-tumor effect with RA on HepG2 treatment.
National Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China; Graduate University of The Chinese Academy of Sciences, Beijing 100049, PR China.
Ye
Y
Jian
Wang
W
Jingze