Protective effect of triphlorethol-A from Ecklonia cava against ionizing radiation in vitro.
We studied the cytoprotective effect of triphlorethol-A against gamma-ray radiation-induced oxidative stress. In this study, hydrogen peroxide, which is a reactive oxygen species (ROS), was detected using 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA) assay. Triphlorethol-A reduced intracellular hydrogen peroxide generated by gamma-ray radiation. This compound provided protection against radiation-induced membrane lipid peroxidation and cellular DNA damage which are the main targets of radiation-induced damage. Triphlorethol-A protected the cell viability damaged by the radiation through inhibition of apoptosis. Triphlorethol-A reduced the expression of bax and activated caspase 3 induced by radiation, but recovered the expression of bcl-2 decreased by radiation. Taken together, the results suggest that triphlorethol-A protects cells against oxidative damage induced by radiation through reducing ROS.
Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju-si, Korea.
Kyoung Ah
KA
Kang
Rui
R
Zhang
Kyoung Hwa
KH
Lee
Sungwook
S
Chae
Bum Joon
BJ
Kim
Young Sook
YS
Kwak
Jae Woo
JW
Park
Nam Ho
NH
Lee
Jin Won
JW
Hyun
D000818:Animals; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects*; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D005720:Gamma Rays; D015227:Lipid Peroxidation / Q000187:drug effects; D000459:Phaeophyceae / Q000378:metabolism*; D010696:Phloroglucinol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D017382:Reactive Oxygen Species / Q000378:metabolism*
D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; C504400:triphlorethol-A; D010696:Phloroglucinol