Antibacterial activity of dental composites containing quaternary ammonium polyethylenimine nanoparticles against Streptococcus mutans.
The antibacterial activity of quaternary ammonium polyethylenimine (PEI) nanoparticles embedded at 1%w/w with clinically used bonding, flowable and hybrid dental composite resins and cured by light polymerization was studied. The antibacterial activity was tested with Streptoccocus mutans by: (i) the agar diffusion test (ADT); (ii) the direct contact test; (iii) bacterial growth in the materials elute; (iv) and scanning electron microscope (SEM). Using the direct contact test, antibacterial activity (p<0.001) was found in all three types of composite resins incorporated with the synthesized nanoparticles. The effect lasted for at least 1 month. SEM demonstrated bacterial debris and no streptococcal chains at 24h of bacterial contact. The addition of 1%w/w of nanoparticles did not affect the flexural modulus and the flexural strength of the dental composite materials. The results indicate that quaternary ammonium PEI nanoparticles immobilized in resin-based materials have a strong antibacterial activity upon contact without leach-out of the nanoparticles and without compromise in mechanical properties.
Department of Prosthodontics, Faculty of Dentistry, The Hebrew University of Jerusalem, Jerusalem, Israel.
Nurit
N
Beyth
Ira
I
Yudovin-Farber
Ran
R
Bahir
Abraham J
AJ
Domb
Ervin I
EI
Weiss
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry*; D003764:Dental Materials / Q000737:chemistry; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry*; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011094:Polyethyleneimine / Q000008:administration & dosage* / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage* / Q000737:chemistry; D013295:Streptococcus mutans / Q000166:cytology / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D003188:Composite Resins; D003764:Dental Materials; D004337:Drug Carriers; D000644:Quaternary Ammonium Compounds; D011094:Polyethyleneimine