[Effect of Herba Epimedii Flavone on expression of OPG and RANKL in rat osteoblasts].
OBJECTIVE
To explore the effect of Herba Epimedii Flavone (HEF) on expression of OPG and RANKL in rat osteoblasts.


METHODS
Osteoblasts were obtained from new born rat calvaria by digestive enzymes. The different HEF concentrations (0.1, 1, 10, 100 ng/ml)were added into the culture medium. Total mRNA was prepared from osteoblasts after 48 h and 96 h, and mRNA expression of OPG and RANKL were detected by RT-PCR.


RESULTS
The mRNA expression of OPG in osteoblasts significantly increased compared with that of the control group, and the V(OPF)/V(RANKL) ratio was deceased markedly in the treatment of 10 ng/ml group after 48 h. After 96 h, the mRNA expression of OPG significantly increased and the V(OPG)/V(RANKL) ratio was also decreased in the treatment group compared with that of the control group.


CONCLUSIONS
HEF suppress the differentiation and maturate of osteoblast. Osteoporosis can be prevented and cured by HEF.
Department of Orthopedics, Second Hospital of Xi' an Jiaotong University, Xi' an.
Yiheng
Y
Liu
Hongmin
H
Zang
Haiying
H
Zhang
Junchang
J
Chen
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D034181:Epimedium / Q000737:chemistry*; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010010:Osteoclasts / Q000187:drug effects; D053244:Osteoprotegerin; D010946:Plants, Medicinal / Q000737:chemistry*; D053245:RANK Ligand; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis* / Q000235:genetics; D013997:Time Factors
D002352:Carrier Proteins; D047309:Flavones; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506032:Tnfrsf11b protein, rat