Effects of long-term storage on potency of TM Biocontrol-1, the registered viral insecticide of Orgyia pseudotsugata (Lepidoptera: Lymantriidae).
The article presents the results of bioassaying 39 samples of TM Biocontrol-1, a viral insecticide, from 10 different lots and various sizes of vacuum-sealed packages that were stored at -10 degrees C for 5-15 yr. This is the first study to present potency data for a registered virus product stored for this length of time. Laboratory bioassays in insects from the same colony from which the TM Biocontrol-1 was produced showed that the stored viral insecticide is still effective, although it lost approximately 30% of its effectiveness during storage. A direct correlation of this loss with the length of time in storage is not apparent. Bioassays also showed that there are significant differences in the susceptibility of Douglas-fir tussock moth, Orgyia pseudotsugata (McDunnough), larvae from different geographic regions to OpMNPV (family Baculoviridae, genus Nucleopolyhedrovirus) infection. Package size did not affect the potency of stored TM Biocontrol-1. There were no clear, significant differences in the effectiveness among lots of TM Biocontrol-1 processed by different organizations.
Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, Victoria, British Columbia V8Z 1M5, Canada.
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Otvos
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Kukan
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Reardon
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Ragenovich
D000818:Animals; D001681:Biological Assay; D001955:British Columbia; D007814:Larva / Q000821:virology; D007928:Lethal Dose 50; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity*; D010572:Pest Control, Biological / Q000379:methods / Q000592:standards*; D011309:Preservation, Biological / Q000379:methods / Q000592:standards*; D013997:Time Factors