A complex of DNA gyrase and RNA polymerase fosters transcription in Mycobacterium smegmatis.
We report here the existence of a complex between RNA polymerase (RNAP) and DNA gyrase in Mycobacterium smegmatis. The interaction between the two enzymes was detected during our attempts to purify DNA gyrase from M. smegmatis. RNAP subunits co-eluted along with DNA gyrase in two different affinity chromatography column procedures employed to purify the latter enzyme. A complex containing both the enzymes was isolated through gel filtration chromatography and sucrose density gradient centrifugation of the cell free extracts. The complex exhibited both DNA supercoiling and transcription activities. Reduction in the transcription activity of the complex in the presence of DNA gyrase inhibitor indicates a role for DNA gyrase in stimulating transcription.
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India.
Richa
R
Gupta
Arnab
A
China
Ujjini H
UH
Manjunatha
N M
NM
Ponnanna
Valakunja
V
Nagaraja
D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D027081:DNA Gyrase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry; D004278:DNA, Superhelical / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D020102:Mycobacterium smegmatis / Q000201:enzymology* / Q000235:genetics; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D004278:DNA, Superhelical; D021122:Protein Subunits; D012321:DNA-Directed RNA Polymerases; D027081:DNA Gyrase