Chemosensitization effects of XIAP downregulation in K562 leukemia cells.
The effect of downregulation of the expression of the antiapoptotic protein XIAP with antisense oligonucleotides was evaluated in the K562 chronic myeloid leukemia (CML) cell line. This was carried out by studying the effects of downregulation of XIAP expression on cellular viability, cellular apoptosis and on the response to two chemotherapeutical drugs, etoposide and doxorubicin. We document that downregulation of XIAP expression decreased cellular viability, increased cellular apoptosis and enhanced the effects of doxorubicin.
Lima
R T
RT
IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Portugal.
Martins
L M
LM
Guimarães
J E
JE
Sambade
C
C
Vasconcelos
M H
MH
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation; D004317:Doxorubicin / Q000494:pharmacology; D005047:Etoposide / Q000494:pharmacology; D006801:Humans; D020014:K562 Cells / Q000187:drug effects / Q000473:pathology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D016376:Oligonucleotides, Antisense; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D005047:Etoposide; D004317:Doxorubicin