Chromatographic analysis of dopamine metabolism in a Parkinsonian model.
The present study examined the metabolism of released dopamine from rat striatum upon chronic rotenone exposure. The sample separation was carried out by two-dimensional, reversed-phase and ion pair reversed-phase chromatography using on-line solid phase extraction enrichment. Reduced dopamine content and decreased extracellular level of [(3)H] and endogenous dopamine evoked by electrical stimulation indicated the injury of dopaminergic pathway. Sensitivity of dopaminergic neurons were increased to oxidative stress with enhanced release of dopamine and formation of oxidized metabolite dopamine quinone (DAQ). Utilizing multidimensional detection, EC at -100 mV reduction potential, the method has been applied for identification of DAQ and aminochrome (DAC).
Baranyi
Mária
M
Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1083, Szigony Street, 43, Budapest, Hungary. baranyi@koki.hu
Milusheva
Elisaveta
E
Vizi
E Sylvester
ES
Sperlágh
Beáta
B
D000227:Adenine Nucleotides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002395:Catecholamines / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D004298:Dopamine / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity; D008297:Male; D008956:Models, Chemical; D015394:Molecular Structure; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012402:Rotenone / Q000633:toxicity
D000227:Adenine Nucleotides; D002395:Catecholamines; D007306:Insecticides; D012402:Rotenone; C104705:dopamine quinone; D004298:Dopamine