Mechanism of hilA repression by 1,2-propanediol consists of two distinct pathways, one dependent on and the other independent of catabolic production of propionate, in Salmonella enterica serovar Typhimurium.
A glycerol dehydrogenase gene was selected as a multicopy suppressor rescuing the reduced hilA expression in the Salmonella enterica serovar Typhimurium cpxA mutant. A substrate of the enzyme, 1,2-propanediol, repressed hilA expression. The 1,2-propanediol-mediated repression at 150 mM, but not that at 300 mM, was abrogated by blocking the catabolism producing propionate from 1,2-propanediol.
Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. shuichin@nih.go.jp
Shu-ichi
S
Nakayama
Haruo
H
Watanabe
D001426:Bacterial Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D011422:Propionates / Q000378:metabolism*; D019946:Propylene Glycol / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism*; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*
D001426:Bacterial Proteins; C102236:HilA protein, Salmonella; D011422:Propionates; D015534:Trans-Activators; D019946:Propylene Glycol; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; C029658:propionic acid