The effect of surface hydrophilicity on the behavior of embryonic cortical neurons.
The aim of this study was to investigate the interaction of mouse embryonic cortical neurons on P(L)LA and PLGA substrates, which were partially hydrolysed using potassium hydroxide (KOH). The chemical and topographical properties of the surfaces were characterized, and it was discovered that there was a decrease in the hydrophilicity for the P(L)LA with increasing concentration of KOH. This was due to chemical modifications to the surfaces of the substrates. Alternatively for the PLGA substrate, only the 0.1 M KOH treated sample had a significantly different hydrophilicity highlighting that surface erosion resulted at higher concentrations. The morphology of the neurons grown on the two substrates were compared to poly(D)lysine (positive control). The neurons formed colonies on all of the substrates, but were dramatically reduced in size in the case of the 0.1 M KOH treated substrates. This finding was attributed to the increases in cell spreading and the size of the cells, as they were larger, more elongated and bipolar like those on the positive control. However, there was a significant decrease in the total number of live cells per unit area. Therefore, on these materials when there was increased cellular spreading there was significantly higher cell death. Furthermore, unlike the 0, 0.2, and 0.4 M KOH treated substrates, there was an absence of large bundles of axons that extended between colonies on the 0.1 M sample, instead exhibiting short axons that grew in free space.
CRC for Polymers, Department of Materials Engineering, Monash University, P.O. Box 69M, Victoria 3800, Australia.
D R
DR
Nisbet
S
S
Pattanawong
J
J
Nunan
W
W
Shen
M K
MK
Horne
D I
DI
Finkelstein
J S
JS
Forsythe
D000818:Animals; D001672:Biocompatible Materials; D018929:Cell Culture Techniques; D002455:Cell Division; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000196:embryology*; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides; D019344:Lactic Acid; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011091:Polyesters; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D017680:Potassium Compounds; D013499:Surface Properties
D001672:Biocompatible Materials; D006878:Hydroxides; D011091:Polyesters; D011108:Polymers; D017680:Potassium Compounds; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); C029943:potassium hydroxide