New insights into BS69 functions.
The BS69 protein has been commonly described as a co-repressor associated with various transcription factors. However, this hypothesis relied predominantly on overexpression of tagged proteins due to the lack of a reliable BS69 antibody. We present for the first time a complete sequence of BS69 and valuable tools to characterize the endogenous protein. We show that the full-length BS69 protein, as well as minor alternatively spliced isoforms, is ubiquitously expressed, nuclear, and associates with chromatin and mitotic chromosomes. Accordingly, BS69 interacts with a set of chromatin remodeling factors, including ATP-dependent helicases, histone deacetylases, and histone methyltransferases, as well as the E2F6 transcription factor. These data strengthen a role for BS69 in gene repression and link BS69 to chromatin remodeling.
INSERM U59O and U412, Centre Léon Berard, Université Claude Bernard Lyon I, 28 rue Laennec, F-69008 Lyon, France.
Guillaume
G
Velasco
Steve
S
Grkovic
Stéphane
S
Ansieau
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D018797:Cell Cycle Proteins; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D011496:Protein Methyltransferases; D025801:Ubiquitin / Q000737:chemistry
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002843:Chromatin; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D020033:Protein Isoforms; D025801:Ubiquitin; C094763:ZMYND11 protein, human; C490622:Zmynd11 protein, mouse; D000076983:Histone Methyltransferases; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase