Inheritance, organization, and mapping of rbcS and cab multigene families in pea.
DNA restriction endonuclease fragment patterns corresponding to both the rbcS and cab multigene families of pea are each shown to segregate as single Mendelian units in the F(2) progeny of two separate crosses. All of the observed variation in each of the multigene families is thus organized on the chromosome in a tightly linked complex. Linkage relationships between both multigene families and an array of morphological and isozyme markers establish the location of the rbcS and cab gene clusters on pea chromosomes 5 and 2, respectively. Our results, which indicate a high level of DNA restriction fragment length polymorphism in pea, suggest sufficient variation to permit the construction of a highly detailed linkage map.
Department of Plant Biology, Carnegie Institution of Washington, 290 Panama Street, Stanford, CA 94305.
N O
NO
Polans
N F
NF
Weeden
W F
WF
Thompson