Layer-by-layer assembly of mucin and chitosan--Influence of surface properties, concentration and type of mucin.
Bovine submaxillary mucin (BSM) and chitosan were used to build layer-by-layer structures on solid substrates. The build-up was monitored using in situ ellipsometry to obtain time resolved values of the thickness and adsorbed amount. Additionally surface morphology during build-up was studied by atomic force microscopy (AFM). It was found that the adsorbed amount of the film increases approximately linearly with each deposition cycle on hydrophobized silica whereas construction on silica was found not to be possible at the experimental conditions used. We conclude that sufficient amount of the first mucin layer is crucial for the subsequent multilayer formation. The complex build-up kinetics on hydrophobized silica is characterized by adsorption and redissolution processes and the overall growth is the sum of both processes. AFM imaging on hydrophobized silica also confirmed the presence of redissolution processes and chitosan addition led to a reduction both in the number of surface aggregates and in the roughness of the surface. The present work also shows that by adjusting the relative concentrations of the polyelectrolytes it is possible to change the growth rate considerably. The final structures after deposition of 8 bilayers were found to have a high content of water and film stability test revealed that a substantial amount dissolves when increasing electrolyte concentration or pH of the ambient solution. Human mucin from saliva (MUC5B) was also used to create multilayers with chitosan on hydrophobized silica and it was revealed that no redissolution appears to be present in this system.
Faculty of Health and Society, Malmö University, SE-20506 Malmö, Sweden. olof.svensson@hs.mah.se
Olof
O
Svensson
Liselott
L
Lindh
Marité
M
Cárdenas
Thomas
T
Arnebrant
D000327:Adsorption; D000818:Animals; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry; D008968:Molecular Conformation; D009077:Mucins / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D012822:Silicon Dioxide; D012996:Solutions; D013499:Surface Properties
D006023:Glycoproteins; D009077:Mucins; D011134:Polysaccharides; D012996:Solutions; D012822:Silicon Dioxide; D048271:Chitosan