[Apoptosis of mesenchymal cell line MBA-1 induced by core binding factor alpha 1].
OBJECTIVE
To investigate the effect of two core binding factors alpha 1 (Cbfa1) isfroms (Cbfa1/P56 and Cbfa1/P57) on the apoptosis of mesenchymal cell line MBA-1.


METHODS
The two Cbfal isfroms were transiently transfected into MBA-1 cells, then the changes of apoptosis rate were observed by flow cytometer. The protein expressions of Cbfa1, Bax, Bcl-2, caspase-3, and caspase-9, cytochrome-C and TNF-alpha were determined by Western immunoblot.


RESULTS
After the transient transfection with the two isforms of Cbfa1, MBA-1, the cells apoptotic rates increased, and the ratio of Bax/Bcl-2, the expressions of cytochrome-C, caspase-3, caspase-9, and TNF-alpha were significantly increased.


CONCLUSIONS
Cbfa1 can promote the apoptosis in mesenchymal cell line MBA-1. Bax/Bcl-2, cytochrome-C, caspase-9, caspase-3, and TNF-alpha are also involved in the apoptosis pathway.
Institute of Endocrinology and Metabolism, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Min
M
Liu
Hou-de
HD
Zhou
Yu-lin
YL
He
Hui
H
Xie
Er-yuan
EY
Liao
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology*; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D050657:Core Binding Factor alpha Subunits / Q000494:pharmacology*; D045304:Cytochromes c / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D020033:Protein Isoforms; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
C494826:BAX protein, human; D050657:Core Binding Factor alpha Subunits; D020033:Protein Isoforms; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C406688:core binding factor alpha; D045304:Cytochromes c; C506433:CASP9 protein, human; C506434:Casp9 protein, mouse; D053453:Caspase 9; D020169:Caspases