Addition of glycerol for improved methylation linkage analysis of polysaccharides.
A presolubilization procedure with the use of glycerol is shown to be applicable for the structural analysis of polysaccharides. Neutral, acidic, high-molecular-weight and low-molecular-weight polysaccharides were solubilized in glycerol prior to methylation and subsequent linkage analysis by GC-MS. All four types of polysaccharides showed significant increases in derivatization following presolubilization as measured by recovery of partially methylated alditol acetates.
Kim
John S
JS
Wyeth Vaccine Research and Development, 4300 Oak Park, Sanford, NC 27330, USA. kimj9@wyeth.com
Reuhs
Bradley L
BL
Michon
Francis
F
Kaiser
Raymond E
RE
Arumugham
Rasappa G
RG
D008401:Gas Chromatography-Mass Spectrometry; D005990:Glycerol / Q000737:chemistry*; D008745:Methylation; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry*; D012995:Solubility; D013402:Sugar Alcohols / Q000737:chemistry
D011134:Polysaccharides; D013402:Sugar Alcohols; D005990:Glycerol