Expression of the glycoprotein of viral haemorrhagic septicaemia virus (VHSV) on the surface of the fish cell line RTG-P1 induces type 1 interferon expression in neighbouring cells.
In the present study using a luciferase/Mx promoter reporter system, it was shown that the rainbow trout gonad cell line (RTG-P1), a fibroblastic cell line, produces IFN when transfected with a plasmid encoding the glycoprotein of VHSV but not with plasmid vector alone. Only a small percentage of the cells expressed the G protein on the surface membrane as indicated by immunostaining of transfected cells. When transfection was performed in the presence of monoclonal antibodies (Mab) to the glycoprotein, the production of interferon mRNA transcripts was reduced by over 50%. This indicates that the surface expression of G protein was the major mechanism of interferon induction and that most of the interferon was being expressed by cells neighbouring the transfected cells.
Department of Animal Pathology, School of Veterinary Medicine, University of Las Palmas de Gran Canaria, Arucas, Las Palmas, Gran Canaria, Spain.
F
F
Acosta
B
B
Collet
N
N
Lorenzen
A E
AE
Ellis
D000818:Animals; D030801:Animals, Genetically Modified; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology*; D007370:Interferon Type I / Q000096:biosynthesis / Q000502:physiology*; D008156:Luciferases / Q000032:analysis; D029141:Novirhabdovirus / Q000502:physiology*; D017686:Oncorhynchus mykiss; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D019444:Vaccines, DNA; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D014765:Viral Vaccines
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D006023:Glycoproteins; D007370:Interferon Type I; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines; D008156:Luciferases