Stabilization of free and immobilized enzymes using hyperthermophilic chaperonin.
Chaperonins suppress the denaturation of proteins and promote protein folding in vivo. Because hyperthermophilic chaperonins are expected to be used as a stabilizer for proteins, the effects of a group II chaperonin from a hyperthermophilic archaeum, Thermococcus strain KS-1 (T. KS-1 cpn), on the stabilization of mesophilic and thermophilic free enzymes and an enzyme co-immobilized with T. KS-1 cpn were studied. T. KS-1 cpn prevented the thermal inactivation of yeast alcohol dehydrogenase (ADH), jack bean urease, and Thermus flavus malate dehydrogenase (MDH) at high temperatures. T. KS-1 cpn also improved the long-term stability of ADH at lower temperatures. Moreover, the residual ADH activity of ADH co-entrapped with T. KS-1 cpn was improved and maintained at a higher level than that of the entrapped ADH without chaperonin. T. KS-1 cpn is useful for the stabilization of free and immobilized enzymes and applicable to various fields of biotechnology.
Kohda
Jiro
J
Department of Information Machines and Interfaces, Faculty of Information Science, Hiroshima City University, 3-4-1 Ozuka-higashi, Hiroshima 731-3194, Japan. kohda@bio.im.hiroshima-cu.ac.jp
Kawanishi
Hirofumi
H
Suehara
Ken-Ichiro
K
Nakano
Yasuhisa
Y
Yano
Takuo
T
D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D018833:Chaperonins / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005782:Gels; D006358:Hot Temperature; D008291:Malate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D019712:Thermococcus / Q000235:genetics; D014510:Urease / Q000737:chemistry / Q000378:metabolism
D004800:Enzymes, Immobilized; D005782:Gels; D011994:Recombinant Proteins; D000426:Alcohol Dehydrogenase; D008291:Malate Dehydrogenase; D014510:Urease; D018833:Chaperonins