[Study on poly A site gene mutation of transforming growth factor-beta1 receptor type II between multiple and single site keloid].
OBJECTIVE
To investigate the pathogenesy deference between multiple and single site keloid by detecting gene mutation of Poly A site of transforming growth factor-beta1 receptor type II (TbetaR II).


METHODS
Collecting 20 keloid samples (6 multiple sites keloid samples and 14 single site keloid samples) and extracting DNA from them; designing and synthesizing the primers of Poly A site, then amplifying T1beta II DNA by PCR, analyzing the single strand conformation polymorphism about the products of PCR. After purifying the product of PCR, the site and type of the mutation rate of Poly A site was sequenced directly on the automatic sequencing equipment.


RESULTS
It had been found that the Poly A site of TbetaR II in keloid has deletion mutation, its mutation rate in multiple sites keloid was 50% (3/6), in single site keloid 7.1% (1/14). The mutation rate of Poly A site in multiple sites keloid was significant higher than that in single site keloid (P < 0.05)


CONCLUSIONS
It has been supposed that there are some deference in pathogenesy between the multiple and the single site keloid.
Department of Plastic, the Second Hospital of Shandong University, Jinan 250033, China.
Jing-long
JL
Cai
Gang
G
An
Bin
B
Xu
Gui-ying
GY
Zhu
Shu-qiang
SQ
Fan
Hong-shan
HS
Yan
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D007627:Keloid / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011061:Poly A / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*
D018125:Receptors, Transforming Growth Factor beta; D011061:Poly A; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II