Development of recombinant chicken IgY from single chain fragment of variable region for diagnosis of BSE.
We generated two recombinant chicken IgYs, designated Ab3-15 and Ab4-19, against mammalian prion protein (PrP) from the single chain fragment of variable region (scFv) antibodies. These two antibodies recognized PrP(Sc) from bovine spongiform encephalopathy (BSE) in cattle and were more sensitive than the corresponding scFv antibodies. These antibodies also recognized PrP(Sc) from other scrapie-infected mammals. These results indicate that Ab3-15 and Ab4-19 are useful for diagnosis of BSE as well as other prion diseases.
Miyamoto
Kazuyoshi
K
Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan.
Shimamoto
Toshi
T
Aosasa
Masayoshi
M
Kimura
Sota
S
Nakamura
Naoto
N
Okubo
Yuka
Y
Yokoyama
Takashi
T
Horiuchi
Hiroyuki
H
Furusawa
Shuichi
S
Matsuda
Haruo
H
D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D002645:Chickens / Q000276:immunology*; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D007136:Immunoglobulins / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification*
C016512:IgY; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; D011994:Recombinant Proteins