[Effects of GM-CSF on the proliferation and activation of dendritic cells in vivo].
OBJECTIVE
To explore the effects of granulocyte macrophage-colony-stimulating factor (GM-CSF) on the proliferation and activation of dendritic cells (DC) in vivo.


METHODS
Balb/c mice were randomly assigned into the control and GM-CSF treatment group. After the lethal dosage of irridiation, green fluorescence protein labelled hematopoietic stem cells (HSC) were injected into the tail veins of the mice. The dosage of 0.1 micorg GM-CSF was administer subcutaneously every 2 days after the HSC infusion. The numbers and activation status of splenic DC were observed by flow cytometry 1, 2, 3, and 4 weeks after the HSC transplantation.


RESULTS
The numbers of splenic DC in the GM-CSF treatment group were significantly higher than those of the control group. The expressions of surface marker CD40, CD80, and CD86 in the GM-CSF treatment group were also higher than those of the control group.


CONCLUSIONS
GM-CSF can enhance the proliferation and activation of DC in vivo.
Institute of Human Reproduction and Stem Cell Engineering, Central South University, Changsha 410078, China.
Wei
W
Liu
Chuan-chang
CC
Li
Guang-xiu
GX
Lu
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D018380:Hematopoietic Stem Cell Transplantation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D013154:Spleen / Q000166:cytology; D014916:Whole-Body Irradiation
D016178:Granulocyte-Macrophage Colony-Stimulating Factor