Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20.
Chronic exercise training elicits adaptations in the heart that improve pump function and confer cardioprotection. To identify molecular mechanisms by which exercise training stimulates this favorable phenotype, a proteomic approach was employed to detect rat cardiac proteins that were differentially expressed or modified after exercise training. Exercise-trained rats underwent six weeks of progressive treadmill training five days/week, 0% grade, using an interval training protocol. Sedentary control rats were age- and weight-matched to the exercise-trained rats. Hearts were harvested at various times (0-72 h) after the last bout of exercise and were used to generate 2-D electrophoretic proteome maps and immunoblots. Compared with hearts of sedentary rats, 26 protein spot intensities were significantly altered in hypertrophied hearts of exercise-trained rats (p <0.05), and 12 spots appeared exclusively on gels from hearts of exercise-trained rats. Immunoblotting confirmed that chronic exercise training, but not a single bout of exercise, elicited a 2.5-fold increase in the abundance of one of the candidate proteins in the heart, a 20 kDa heat shock protein (hsp20) that persisted for at least 72 h of detraining. Thus, exercise training alters the cardiac proteome of the rat heart; the changes include a marked increase in the expression of hsp20.
Boluyt
Marvin O
MO
Center for Exercise Research, Division of Kinesiology, The University of Michigan, Laboratory of Molecular Kinesiology, 401 Washtenaw Avenue, Ann Arbor, MI 48109, USA. boluytm@umich.edu
Brevick
Julie L
JL
Rogers
David S
DS
Randall
Michael J
MJ
Scalia
Antony F
AF
Li
Zhao Bo
ZB
D000818:Animals; D001835:Body Weight; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D050886:HSP20 Heat-Shock Proteins / Q000096:biosynthesis*; D006321:Heart / Q000033:anatomy & histology; D015151:Immunoblotting; D013058:Mass Spectrometry; D009124:Muscle Proteins / Q000096:biosynthesis*; D009206:Myocardium / Q000378:metabolism*; D009929:Organ Size; D010766:Phosphorylation; D010805:Physical Conditioning, Animal; D020543:Proteome / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
D050886:HSP20 Heat-Shock Proteins; D009124:Muscle Proteins; D020543:Proteome; C087458:Hspb6 protein, rat