Rap-GEF/Rap signaling restricts the formation of supernumerary spermathecae in Drosophila melanogaster.
Sperm storage in the female is a key factor for reproductive success in a variety of organisms, including Drosophila melanogaster. The spermathecae (SP) are the Drosophila organs for long-term storage. While wild-type female flies have two SP, occasionally, three or four SP have been observed in mutant flies. However, the molecular mechanism of SP formation is unknown. Here we show that loss of function of a Drosophila Rap-GEF (GEF26) result in an occurrence of the supernumerary SP; females have three SP (varies from 11 to 62% in different allele combinations) instead of the normal two SP. In addition, the Gef26 mutant flies also have ectopic wing veins and extra mechanosensory organs. The supernumerary SP phenotype of the Gef26 mutation can be enhanced by the Drosophila Rap mutations and rescued by overexpressing the cell adhesion molecule DE-cadherin. These data suggest that the Rap-GEF/Rap signaling controls the formation of supernumerary spermathecae through modulating cell adhesion in Drosophila.
Singh
Shree R
SR
Mouse Cancer Genetics Program, National Institutes of Health, National Cancer Institute at Frederick, Frederick, MD 21702, USA. sshreeram@ncifcrf.gov
Oh
Su-Wan
SW
Liu
Wei
W
Chen
Xiu
X
Zheng
Zhiyu
Z
Hou
Steven X
SX
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D004331:Drosophila melanogaster / Q000254:growth & development*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005836:Genitalia, Female / Q000254:growth & development*; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D012098:Reproduction / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D020668:rap GTP-Binding Proteins / Q000502:physiology*
D020662:Guanine Nucleotide Exchange Factors; D012333:RNA, Messenger; D020668:rap GTP-Binding Proteins