Profiling urinary metabolites of naproxen by liquid chromatography-electrospray mass spectrometry.
Glucuronidation, an important metabolic process for the biotransformation of drugs into easily eliminable water-soluble detoxification products, can also lead to biologically active or toxic glucuronide conjugates. The present work describes a liquid chromatography-electrospray mass spectrometry (LC-ESI-MS) approach for the characterization of naproxen and O-6-desmethylnaproxen glucuronides. The method is fast and efficient and permitted to individuate alpha and beta isomers of both naproxen and O-6-desmethylnaproxen glucuronides. The procedure could be potentially extended to the characterization of other drug metabolites.
Centro Interdipartimentale di Ricerca "Spettrometria di Massa Analitica per Ricerche Tecnologiche (SMART)" Università degli Studi di Bari, Dipartimento di Chimica, Via Orabona 4, 70126-BARI, Italy.
Antonella
A
Aresta
Teresa
T
Carbonara
Francesco
F
Palmisano
Carlo G
CG
Zambonin
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism* / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods*; D020719:Glucuronides / Q000652:urine; D006801:Humans; D009288:Naproxen / Q000031:analogs & derivatives / Q000378:metabolism* / Q000652:urine; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D020719:Glucuronides; C430408:naproxen glucuronide; D009288:Naproxen