Mechano-active scaffold design based on microporous poly(L-lactide-co-epsilon-caprolactone) for articular cartilage tissue engineering: dependence of porosity on compression force-applied mechanical behaviors.
An essential component of functional articular cartilage tissue engineering is a mechano-active scaffold, which responds to applied compression stress and causes little permanent deformation. As the first paper of a series on mechano-active scaffold-based cartilage tissue engineering, this study focused on mechanical responses to various modes of loading of compression forces and subsequent selection of mechano-active scaffolds from the biomechanical viewpoint. Scaffolds made of elastomeric microporous poly(L-lactide-co-epsilon-caprolactone) (PLCL) with open-cell structured pores (300 approximately 500 microm) and with different porosities ranging from 71 to 86% were used. The PLCL sponges and rabbit articular cartilage tissue were subjected to compression/unloading tests (0.1 and 0.005 Hz) at 5 kPa, and stress relaxation tests at 10, 30, and 50% strain. The measurements of the maximum strain under loading and residual strain under unloading for compression tests and the maximum stress and equilibrium stress in the stress relaxation test showed that the lower the porosity, the closer the mechanical properties are to those of native cartilage tissue. Among the PLCL sponges, the sponge with 71% porosity appears to be a suitable cartilage scaffold.
Division of Biomedical Engineering, Graduate School of Medicine, Kyushu University, Higashi-ku, Fukuoka, Japan.
Jun
J
Xie
Maki
M
Ihara
Youngmee
Y
Jung
Il Keun
IK
Kwon
Soo Hyun
SH
Kim
Young Ha
YH
Kim
Takehisa
T
Matsuda
D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D002358:Cartilage, Articular / Q000502:physiology* / Q000601:surgery*; D019245:Compressive Strength; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D011817:Rabbits; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D011091:Polyesters; C062968:lactide-caprolactone copolymer