Quantitation of seven polyoxypregnane glycosides in Marsdenia tenacissima using reversed-phase high-performance liquid chromatography-evaporative light-scattering detection.
A high-performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD) has been developed for the simultaneous determination of seven polyoxypregnane glycosides, tenacissosides A, B, G, H, I and marsdenosides C, G, in the stem of Marsdenia tenacissima, a Chinese herbal medicine. With a C18 analytical column, the analytes were separated efficiently using methanol-water as the mobile phase in a gradient program. The method limits of detection ranged from ca. 0.3 microg for marsdenoside C to ca. 0.5 microg for marsdenoside G and the method limits of quantitation from 1.0 microg for marsdenoside C to 1.7 microg for marsdenoside G, respectively. The intra- and inter-day precisions of the method were evaluated and all were less than 4%. All the recoveries for the spiked analytes exceeded 90%. This method was successfully used to analyze 19 samples of the stem of M. tenacissima.
Deng
Jun
J
Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 200032, China.
Shen
Fan
F
Chen
Daofeng
D
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D008027:Light; D031101:Marsdenia / Q000737:chemistry*; D011278:Pregnanes / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity
D006027:Glycosides; D011278:Pregnanes