[Determination of four components in root of Saposhnikovia divaricata by HPLC gradient elution].
OBJECTIVE
To develop an HPLC method for determination of components in root of Saposhnikovia divaricata.


METHODS
The separation of four components was achieved on a reversed-phase Alltima C18 column with MeOH-H2O gradient elution at a flow rate of 1.0 mL x min(-1).


RESULTS
Six samples were determined and there is no distinct difference between the contents determined by the new and old methods.


CONCLUSIONS
The new method is more simple and convenient than the old method.
Li
Li
L
Institute of Chinese Materia Media, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Liu
Yuan-Yan
YY
Geng
Li-Dong
LD
Xiao
Yong-Qing
YQ
D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D009005:Monosaccharides / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D014966:Xanthenes / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D009005:Monosaccharides; D014966:Xanthenes; C498945:prim-O-glucosylcimifugin