[Primary culture of human omental preadipocytes].
OBJECTIVE
To establish a primary culture method of human omental preadipocytes.


METHODS
Using enzyme-digesting method, fibroblast-like cells from the human omental adipose tissues were cultured, and then differentiated by conditional medium, and identified by oil red O staining.


RESULTS
The cultured cells were highly homogeneous, and highly proliferative in 4-5th generation. During the process of induction by conditional medium, the cells became round-like and larger, and more adipose droplets were aggregated. By oil red O staining, we confirmed the differentiated cells were mature adipocytes.


CONCLUSIONS
In human omental adipose tissues, there are some preadipocytes, which can differentiate into mature adipocytes with appropriate stimulus.
Sheng
Zhi-feng
ZF
Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China. szf1976@hotmail.com
Wang
Ping-fang
PF
Wang
Wei
W
He
Jing
J
Li
Wen-jing
WJ
D017667:Adipocytes / Q000166:cytology*; D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D006801:Humans; D008297:Male; D009852:Omentum / Q000166:cytology*
D017077:Culture Media, Conditioned