Control of the oxygen dependence of an implantable polymer/enzyme composite biosensor for glutamate.
Biosensors for glutamate (Glu) were fabricated from Teflon-coated Pt wire (cylinders and disks), modified with the enzyme glutamate oxidase (GluOx) and electrosynthesized polymer PPD, poly(o-phenylenediamine). The polymer/enzyme layer was deposited in two configurations: enzyme before polymer (GluOx/PPD) and enzyme after polymer (PPD/GluOx). These four biosensor designs were characterized in terms of response time, limit of detection, Michaelis-Menten parameters for Glu (J max and K(M)(Glu)), sensitivity to Glu in the linear response region, and dependence on oxygen concentration, K(M)(O2). Analysis showed that the two polymer/enzyme configurations behaved similarly on both cylinders and disks. Although the two geometries showed different behaviors, these differences could be explained in terms of higher enzyme loading density on the disks; in many analyses, the four designs behaved like a single population with a range of GluOx loading. Enzyme loading was the key to controlling the K(M)(O2) values of these first generation biosensors. The counterintuitive, and beneficial, behavior that biosensors with higher GluOx loading displayed a lower oxygen dependence was explained in terms of the effects of enzyme loading on the affinity of GluOx for its anionic substrate. Some differences between the properties of surface immobilized GluOx and glucose oxidase are highlighted.
UCD School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
Colm P
CP
McMahon
Gaia
G
Rocchitta
Pier A
PA
Serra
Sarah M
SM
Kirwan
John P
JP
Lowry
Robert D
RD
O'Neill
D000594:Amino Acid Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004800:Enzymes, Immobilized; D005971:Glutamates / Q000032:analysis*; D007700:Kinetics; D010101:Oxygen Consumption / Q000502:physiology*; D010984:Platinum / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry*; D013499:Surface Properties
D004800:Enzymes, Immobilized; D005971:Glutamates; D010984:Platinum; D011094:Polyethyleneimine; D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase