Evaluation of Pluchea carolinensis extracts as antioxidants by the epinephrine oxidation method.
Many methods have been used to evaluate synthetic and natural products as hydroxyl radical scavengers. In this study a spectrophotometric method to evaluate the scavenger activity of the hydroxyl radical produced in Fenton reaction is proposed. The oxidation of l-epinephrine to adrenochrome permitted the detection of the hydroxyl radical by spectrophotometry and its possible scavenging by hydroquinone, a well known antioxidant. The method was used to evaluate the antioxidant activity of Pluchea carolinensis leaves.
Fernández
F
F
Department of Applied Science, Matanzas University, Zaragoza 8506 entre Río y Medio, Matanzas, Cuba. fernando.fernandez@infomed.sld.cu
Torres
M
M
D000975:Antioxidants / Q000737:chemistry*; D019659:Asteraceae / Q000737:chemistry*; D004837:Epinephrine / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry; D006873:Hydroquinones / Q000737:chemistry; D017665:Hydroxyl Radical / Q000378:metabolism*; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods
D000975:Antioxidants; D016166:Free Radical Scavengers; D006873:Hydroquinones; D010636:Phenols; D010936:Plant Extracts; D017665:Hydroxyl Radical; C031927:hydroquinone; D004837:Epinephrine