Teaching molecular genetics: Chapter 3--Proteomics in nephrology.
The novel discipline of proteomics has experienced a rapid growth in the recent past and has great potentials for the future. The study of proteins on a genomic scale enables a large number of proteins to be analysed simultaneously. Moreover, proteomic analysis reveals the presence of protein isoforms and post-translational modifications, both of which have the potential to regulate protein complex formation, activity and function. As such, the assessment of the proteome, unlike genomic analysis, provides a view of biological processes at their level of occurrence. The knowledge thus gained is important not only for a better understanding of renal physiology and pathophysiology, but also for the identification of disease markers and the development of new therapies. This review applies the science of proteomics to nephrology: our aim is to give an overview of the discipline, providing background information and outlining the scope, advantages and limitations of proteomics.
Groenen
Patricia J T A
PJ
Department of Pathology, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands. P.Groenen@pathol.umcn.nl
van den Heuvel
Lambert P W J
LP
D015415:Biomarkers / Q000032:analysis* / Q000652:urine; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007674:Kidney Diseases / Q000503:physiopathology*; D013058:Mass Spectrometry / Q000379:methods*; D008967:Molecular Biology; D009398:Nephrology; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014556:Urine / Q000737:chemistry
D015415:Biomarkers; D020543:Proteome