[Screening of glucosidase inhibitors from various fractions of Mulberry leaves].
OBJECTIVE
To search for glucosidase inhibitors of various fractions extracted from mulberry leaves.


METHODS
The constituents of mulberry leaves water fraction were prepared by the process of boiling, condensing, precipitating, exchanging with resins and rinsing. In vitro glucosidase inhibitory activities were examined by photometric bioassay derived from rats. To investigate in vivo effect of lowering blood glucose, the mouse blood glucose level was assayed by glucose tolerance experiments.


RESULTS
The glucosidase inhibitory activities were found in all the constituents of alkaloids, flavones and amyloses, the alkaloid constituent being the strongest.


CONCLUSIONS
The effect of reducing blood glucose of mulberry leaves is related to the inhibitory activities against glucosidase of different constituents.
Department of Nephrology, Tongji Hospital of Tongji University, Shanghai 200065, China. aihongyuanxn00@yahoo.com.cn
Ai-Hong
AH
Yuan
Jun
J
Ma
Xiao-Feng
XF
Jiang
Su
S
Li
D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology; D000688:Amylose / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology; D005951:Glucose Tolerance Test; D065089:Glycoside Hydrolase Inhibitors; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D031625:Morus / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D000520:alpha-Glucosidases / Q000378:metabolism
D000470:Alkaloids; D047309:Flavones; D065089:Glycoside Hydrolase Inhibitors; D000688:Amylose; D000520:alpha-Glucosidases