Towards biochemical reaction monitoring using FT-IR synchrotron radiation.
A lab-on-a-chip device made of CaF2 windows and SU-8 polymer was used for fluid lamination to achieve rapid mixing of two streamlines with a cross section of 300 x 5 microm each. Time resolved measurements of the induced chemical reaction was achieved by applying constant feeding low flow rates and by on-chip measurement at defined distances after the mixing point. Synchrotron IR microscopic detection was employed for direct and label-free monitoring of (bio)chemical reactions. Furthermore, using synchrotron IR microscopy the measurement spot could be reduced to the diffraction limit, thus maximizing time resolution in the experimental set-up under study. Based on computational fluid dynamic simulations the principle of the set-up is discussed. Experimental results on the basic hydrolysis of methyl chloroacetate proved the working principle of the experimental set-up. First results on the interaction between the antibiotic vancomycin and a tripeptide (Ac2KAA) involved in the build up of the membrane proteins of gram-positive bacteria are presented.
Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9-164, 1060 Vienna, Austria.
N
N
Kaun
S
S
Kulka
J
J
Frank
U
U
Schade
M J
MJ
Vellekoop
M
M
Harasek
B
B
Lendl
D000818:Animals; D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D017022:Flow Injection Analysis; D007090:Image Interpretation, Computer-Assisted; D015591:Microspectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D017356:Synchrotrons