[Screening, identification of the strain Fds-1 for microbial desulfurization specially and its use in diesel oil desulfurization].
A strain Fds-1, which can specially break the C-S bond of dibenzothiophene (DBT) and convert DBT into 2-hydrobenzophene (2-HBP), was screened out from soil soaked with crude oil and identified as Bacillus subtilis. Both Gibb's reaction and GC-MS analysis proved that this bacterium could remove sulfur of DBT by "4S" pathway. The optimal temperature of desulfurization is 30 degrees C. At this temperature, Fds-1 could remove 0.5mmol/L organ sulfur of DBT in 72h, so it is suitable to decrease the sulfur content of diesel oil. The final product named 2-HBP is a water-soluble compound. This research focused on the desulfurization methods of diesel oil using Fds-1 resting cells. The results showed Fds-1 exhibited strong degradation capacity of DBT and alkyl-DBT in diesel oil. Experiments of two diesel oil samples for desulfurization proved that Fds-1 did not break the carbon-carbon backbone and the calorific value of diesel oil was reserved. Therefore strain Fds-1 is significant to deep biodesulfurisation comparing the hydro-desulfurization of diesel oil.
College of Life Sciences, NanKai University, Tianjin 300071, China. tingma@nankai.edu.cn
Ting
T
Ma
Ming-you
MY
Tong
Quan
Q
Zhang
Feng-lai
FL
Liang
Ru-lin
RL
Liu
D001412:Bacillus subtilis / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D010578:Petroleum / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S; D012988:Soil Microbiology; D013876:Thiophenes / Q000378:metabolism*
D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D013876:Thiophenes; C016366:dibenzothiophene