The adult mouse utricle as an in vitro preparation for studies of ototoxic-drug-induced sensory hair cell death.
Sensory hair cells of the inner ear are susceptible to death from a variety of stresses including aging, noise trauma, genetic disorders, and exposure to certain therapeutic drugs. Ototoxic drugs include the aminoglycoside antibiotics and the antineoplastic agent cisplatin. This is a short technical report describing the dissection and culture of the adult mouse utricle. This in vitro preparation allows for detailed studies of ototoxic-drug-induced hair cell death in an adult mammalian system. In addition, this preparation allows for examination of the effects of specific gene products through the use of transgenic and knockout mouse models.
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, PO Box 250908, Charleston, SC 29425, USA. cunninll@musc.edu
Lisa L
LL
Cunningham
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D016923:Cell Death / Q000187:drug effects; D002945:Cisplatin / Q000633:toxicity*; D018069:Hair Cells, Vestibular / Q000187:drug effects*; D066298:In Vitro Techniques; D051379:Mice; D012444:Saccule and Utricle / Q000166:cytology*
D000900:Anti-Bacterial Agents; D002945:Cisplatin