[Nude mice intraperitoneally transfused with ex vivo expanded bone marrow CD34+ CD59+ cells from patients with paroxysmal nocturnal hemoglobinuria].
Ex vivo expanded human bone marrow CD34(+)CD59(+) cells from patients with paroxysmal nocturnal hemoglobinuria (PNH) were transplanted into BALB/c mice in order to investigate their proliferation ability and reconstruction of hemopoiesis, and to lay the groundwork for clinical ABMT/APBSCT in PNH patients. CD34(+)CD59(+) cells were selected from the bone marrow mononuclear cells in PNH patients by using immunomagnetic positive double sorting. Sublethally irradiated BALB/c mice were transplanted with CD34(+)CD59(+) cells enriched from bone narrow of PNH patients. The results showed that human CD45(+) cells were detected in the bone marrow, spleen and peripheral blood of the nude mice by flow cytometry and DNA analysis at 6 weeks post-transplant. Blood routine indicators of nude mice were found to recover to some extent, but did not fully recover. It is concluded that ex vivo expanded bone marrow CD34(+)CD59(+) cells from patients with paroxysmal nocturnal hemoglobinuria could keep their biological characteristics and ability to reconstruct hemopoiesis in irradiated BALB/c mice.
Department of Hematology, Beijing Chaoyang Hospital Affiliated of Capital University of Medical Sciences, Bejing 100043, China.
Yu-Ping
YP
Zhong
Yong-Ji
YJ
Wu
Ti
T
Shen
Xuan
X
Wang
Jie-Ping
JP
Zhang
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis*; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology; D018957:CD59 Antigens / Q000032:analysis*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006457:Hemoglobinuria, Paroxysmal / Q000473:pathology / Q000628:therapy*; D006801:Humans; D018189:Immunomagnetic Separation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014183:Transplantation, Heterologous
D018952:Antigens, CD34; D018957:CD59 Antigens