[Effect of adeno-BMP7 transfection on osteogenesis of BMSCs].
OBJECTIVE
To study the effect of adeno-BMP7 transfection on the biology of bone marrow stromal cells (BMSCs).


METHODS
Bone marrow was obtained from the goat. The BMSCs were isolated and cultured at the second passage. Once the cells attached and formed a monolayer with 70%-80% confluency, adeno-BMP7 (M.O.I. = 100) was added to the cells. After three days, calcium node was examined with staining; cell-coral compound was replanted subcutaneously.


RESULTS
With adeno-BMP7 transfection, BMP7 expression was detected with Western-blot; big calcium nodes were observed with staining. New bone formation was enhanced, which was evaluated by X-ray and histological examinations.


CONCLUSIONS
BMSCs transfected with adeno-BMP7 show much stronger osteogenic ability.
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiaotong Medical University and Shanghai Tissue Engineering Center, Shanghai 200011, China.
Lian
L
Zhu
Fu-guo
FG
Chen
Wei
W
Liu
Lei
L
Cui
Guang-dong
GD
Zhou
De-li
DL
Liu
Yi-lin
YL
Cao
D000256:Adenoviridae; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics*; D001861:Bone Regeneration; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D006041:Goats; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010012:Osteogenesis; D023822:Tissue Engineering / Q000379:methods; D014162:Transfection
D055419:Bone Morphogenetic Protein 7