An integrated morphological and molecular approach to a new species description in the Trypanosomatidae: the case of Leptomonas podlipaevi n. sp., a parasite of Boisea rubrolineata (Hemiptera: Rhopalidae).
Leptomonas podlipaevi n. sp., a new trypanosomatid species, is described herein based on light microscopic, ultrastructural, and molecular phylogenetic data. The organism is pleomorphic both in host and culture, with two predominant forms-a typical promastigote with a long flagellum and a shorter promastigote with a small or barely extending flagellum. Several spliced leader RNA repeat sequences obtained from the original cultures and the clonal lines representing two types of cells were all nearly identical. These sequences formed a tight cluster in the neighbor-joining tree well separated from other trypanosomatid species. Glyceraldehyde phosphate dehydrogenase gene sequences were determined for L. podlipaevi and 10 previously described trypanosomatid species. Molecular phylogenetic analysis has demonstrated that the new species is most closely related to Leptomonas seymouri and Leptomonas pyrrhocoris. The analysis has also highlighted the polyphyly of the genus Leptomonas.
Yurchenko
Vyacheslav
V
Albert Einstein College of Medicine of Yeshiva University, Bronx, New York, New York 1046 USA.
Lukes
Julius
J
Xu
Xing
X
Maslov
Dmitri A
DA
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006430:Hemiptera / Q000469:parasitology*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020038:RNA, Spliced Leader / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014351:Trypanosomatina / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure
D016054:DNA, Protozoan; D020038:RNA, Spliced Leader; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases