Comparison of silicon-coated nylon suture to plain nylon suture in the rat middle cerebral artery occlusion model.
A variety of intraluminal sutures have been used in the middle cerebral artery occlusion model (MCAO) of focal ischemia. In the present study we tested commercially available silicon-coated nylon suture in the MCAO model and compared the results to traditional monofilament nylon suture occlusion. Twelve Sprague-Dawley male rats were randomly divided two groups, MCAO with 4-0 nylon suture (Group N, n=6) and MCAO with silicone-coated 4-0 nylon suture (Group S, n=6). Rats were sacrificed 24 h after reperfusion. Assessment included mortality rates, neurological evaluation, and infarct volume. One rat died in each group from subarachanoid hemorrhage. Neurological evaluation demonstrated that Group S tended to have worse neurological outcomes than Group N, although this difference was not statistically significant. On TTC stain Group S had significantly larger infarct volumes than Group N. We conclude that the commercially available silicone-coated occlusion suture provides better occlusion of the middle cerebral artery than the traditional uncoated nylon suture.


METHODS
Disease-related neuroscience (Section 6).
Shimamura
Norihito
N
Department of Physiology and Pharmacology, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA.
Matchett
Gerald
G
Tsubokawa
Tamiji
T
Ohkuma
Hiroki
H
Zhang
John
J
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001921:Brain / Q000473:pathology; D002545:Brain Ischemia / Q000473:pathology / Q000523:psychology; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology* / Q000523:psychology; D008026:Ligation; D008297:Male; D020768:Middle Cerebral Artery / Q000502:physiology*; D009757:Nylons; D051381:Rats; D017207:Rats, Sprague-Dawley; D012825:Silicon; D013537:Sutures
D009757:Nylons; D012825:Silicon