Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1.
The elimination of viruses and tumors by natural killer cells is mediated by specific natural killer cell receptors. To study the in vivo function of a principal activating natural killer cell receptor, NCR1 (NKp46 in humans), we replaced the gene encoding this receptor (Ncr1) with a green fluorescent protein reporter cassette. There was enhanced spread of certain tumors in 129/Sv but not C57BL/6 Ncr1(gfp/gfp) mice, and influenza virus infection was lethal in both 129/Sv and C57BL/6 Ncr1(gfp/gfp) mice. We noted accumulation of natural killer cells at the site of influenza infection by tracking the green fluorescent protein. Our results demonstrate a critical function for Ncr1 in the in vivo eradication of influenza virus.
The Lautenberg Center for General and Tumor Immunology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel.
Roi
R
Gazit
Raizy
R
Gruda
Moran
M
Elboim
Tal I
TI
Arnon
Gil
G
Katz
Hagit
H
Achdout
Jacob
J
Hanna
Udi
U
Qimron
Guy
G
Landau
Evgenia
E
Greenbaum
Zichria
Z
Zakay-Rones
Angel
A
Porgador
Ofer
O
Mandelboim
D000818:Animals; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D055627:Natural Cytotoxicity Triggering Receptor 1; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D011971:Receptors, Immunologic / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D015996:Survival Rate
C112260:NCR1 protein, human; D055627:Natural Cytotoxicity Triggering Receptor 1; D011971:Receptors, Immunologic; D049452:Green Fluorescent Proteins