Human beta-defensin 2 induces a vigorous cytokine response in peripheral blood mononuclear cells.
beta-Defensins are a family of small cationic peptides involved in the innate response to microbial infection. Although their role in microbial killing is well established, the mechanisms through which this occurs remain largely undefined. Here, using protein array technology, we describe a role for human beta-defensins in the induction of an inflammatory cytokine response by human peripheral blood mononuclear cells (PBMCs). Human beta-defensins 1, 2, and 3 were examined for induction of an array of cytokines and chemokines. Some cytokines, such as interleukin 8 (IL-8) and monocyte chemoattractant protein 1, were up-regulated by all three defensins, while others, such as IL-6 and IL-10, were induced more selectively. It was notable that each defensin induced a unique pattern of cytokines. This report documents, for the first time, an analysis of the composite cytokine response of human PBMCs to beta-defensins. The induction or up-regulation of a number of cytokines involved in the adaptive immune response suggests a possible role for these defensins in linking innate and acquired immunity.
Department of Oral Biology, University of Medicine and Dentistry of New Jersey, MSB, Rm. C-636, 185 South Orange Avenue, Newark, NJ 07103-2714, USA. boniotto@bbcm.units.it
Michele
M
Boniotto
William J
WJ
Jordan
Joyce
J
Eskdale
Alessandro
A
Tossi
Nikolinka
N
Antcheva
Sergio
S
Crovella
Nancy D
ND
Connell
Grant
G
Gallagher
D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D023083:beta-Defensins / Q000494:pharmacology*
D016207:Cytokines; C406407:DEFB1 protein, human; C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D012333:RNA, Messenger; D023083:beta-Defensins; D016753:Interleukin-10