Two putative c-type multiheme cytochromes required for the expression of OmcB, an outer membrane protein essential for optimal Fe(III) reduction in Geobacter sulfurreducens.
Deletion of two homologous Geobacter sulfurreducens c-type cytochrome genes, omcG and omcH, decreased the rate of Fe(III) reduction and decreased the level of an outer membrane cytochrome critical for Fe(III) reduction, OmcB, without affecting its transcription. Expression of either gene restored Fe(III) reduction and OmcB expression, suggesting functional similarity.
Kim
Byoung-Chan
BC
Department of Microbiology, 203 Morrill Science Center IVN, University of Massachusetts at Amherst, 639 North Pleasant Street, Amherst, MA 01003, USA. bckim@microbio.umass.edu
Qian
Xinlei
X
Leang
Ching
C
Coppi
Maddalena V
MV
Lovley
Derek R
DR
D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000096:biosynthesis; D015152:Blotting, Northern; D015153:Blotting, Western; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism*; D017353:Gene Deletion; D015870:Gene Expression; D005798:Genes, Bacterial; D045302:Geobacter / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D005290:Ferric Compounds; C436968:OmcB protein, bacteria; D012329:RNA, Bacterial; D012333:RNA, Messenger; D045304:Cytochromes c