[Acute toxicity and immunoprotection of recombinant apxI toxin of Actinobacillus pleuropneumoniae in mice].
Acute toxicity and immunoprotection of Actinobacillus pleuropneumoniae (APP) ApxI toxin recombinant proteins (include crude inclusion bodies and refolded recombinant protein) were evaluated in mice, and compared with the natural ApxI extracted from culture supernatant of APP serotype 10. In the acute toxicity experiment, the three proteins were intraperitoneally injected into Kunming mice in a dose of 200microg per mouse. The body and organ weight, heamatological and biochemical indexes were examined at 24h, 7 days and 14 days post administration. There was no death after the intraperitoneal administration of the three proteins, and no significant change was found in the body weight, organ indexes, heamatological and biochemical indexes. To study their immunoprotection, the three proteins were emulsified with Freund's adjuvant respectively and vaccinated the mice twice with a 2-week of interval. Two weeks after the second vaccination, the mice were challenged intraperitoneally with a lethal dose of APP serotype 10 (1.09 x 10(8) cfu), and serums were examined by an ApxI-specific ELISA. The results revealed that the recombinant protein had a good immunogenicity and could induce protection immune reaction.
Hubei Key Laboratory of Preventive Veterinary Medicine, Wuhan 430070, China.
Ke-Xia
KX
Yan
Jian-Jie
JJ
Liu
Rui
R
Zhou
Bin
B
Wu
Wei-Hong
WH
Liu
Huan-Chun
HC
Chen
D000189:Actinobacillus Infections / Q000517:prevention & control; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D008297:Male; D051379:Mice; D011897:Random Allocation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D023382:Toxicity Tests, Acute
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C087744:ApxI toxin, Bacteria