Application of headspace solid phase microextraction to qualitative and quantitative analysis of tobacco additives in cigarettes.
Cigarettes may contain up to 10% by weight additives which are intended to make them more attractive. A fast and rugged method for a cigarette-screening for additives with medium volatility was developed using automatic headspace solid phase microextraction (HS-SPME) with a 65 microm carbowax-divinylbenzene fiber and gas chromatography-mass spectrometry (GC-MS) with standard electron impact ionisation. In three runs, each cigarette sample was extracted in closed headspace vials using basic, acidic and neutral medium containing 0.5 g NaCl or Na2SO4. Furthermore, the method was optimized for quantitative determination of 17 frequently occurring additives. The practical applicability of the method was demonstrated for cigarettes from 32 brands.
Institute of Legal Medicine, Humboldt-University, Hittorfstr. 18, D-14195 Berlin, Germany.
Careen
C
Merckel
Fritz
F
Pragst
Astrid
A
Ratzinger
Beat
B
Aebi
Werner
W
Bernhard
Frank
F
Sporkert
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D012015:Reference Standards; D014026:Nicotiana / Q000737:chemistry*