[Determination of salidroside and p-tyrosol in Hongjingtian for injection(freezing-dry) by SPE-HPLC].
OBJECTIVE
To assay salidroside and p-tyrosol in Hongjingtian for injection (freezing-dry).


METHODS
Samples were purified by Sep-Pak C18 column and salidroside and p-tyrosol were determined by HPLC with Irregular-H C18 (4.6 mm x 250 mm, 5 microm), and eluted with a mobile phase of methanol-acenitonitrile -0.06% phosphate (10: 10:80). The flow rate was 1.0 mL x min(-1), the detection wavelength was set at 275 nm and the column temperature was maintained at 30 degrees C.


RESULTS
The calibration curves were linear in the range of 2.24-22.4 microg for salidroside (0.999 7) and 0.856-8.56 microg for p-tyrosol (0.999 6), the average recovery was 101.3%, 99.8% respectively.


CONCLUSIONS
The method is convenient, rapid, accurate and reliable.
Meng
Jian
J
Pharmaceutical College of Shenyang Pharmaceutical University, Shenyang 110016, China.
Wang
Shu-Fen
SF
Han
Fei
F
Li
San-Ming
SM
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D007267:Injections; D010636:Phenols / Q000032:analysis*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D029771:Rhodiola / Q000737:chemistry*; D013696:Temperature
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010636:Phenols; C011867:4-hydroxyphenylethanol; C009172:rhodioloside; D010626:Phenylethyl Alcohol