[Screening and detecting of proteins interacting with mPem].
mPem, a homeobox gene, is expressed in a time and stage specific manner during murine ontogeny. Pem transcripts are abundant in 7- and 8-day mouse embryos, but decrease precipitously thereafter. On Day 9 they become abundant in placenta and yolk sac, persisting there until parturition. Although Pem transcripts are not detectable in most of adult tissues, they are present in reproductive system such as testis, epididymis and ovary. This indicates a important role for Pem during embryogenesis and reproductive development. To study the function of mPem protein, we used a GAL4 based yeast two-hybrid assay to screen a 7-day mouse embryo library with full-length of mPem. 3 proteins were found interacting with mPem protein. One of theses is Mdfic. We confirmed the interaction between mPem and Mdfic in yeast and in vitro. Mdfic, MyoD family inhibitor domain containing, encodes the myoD family inhibitor domain (I-mfa domain). The interaction between mPem and Mdfic suggested they maybe form the transcriptional regulator complex to regulate embryo differentiation.
Luo
Zhi-Wen
ZW
College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Guo
Fen
F
Li
Yue-Qin
YQ
Li
Shi-Qian
SQ
Zhang
Xin
X
Li
Hong-Jian
HJ
Zhou
Tian-Hong
TH
D000818:Animals; D004622:Embryo, Mammalian; D047108:Embryonic Development; D005260:Female; D005801:Genes, Homeobox / Q000235:genetics*; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D011247:Pregnancy; D011485:Protein Binding; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D018398:Homeodomain Proteins; D014157:Transcription Factors; C498048:Rhox5 protein, mouse