The effect of propofol on astro- and microglial reactivity in the course of experimental intracerebral haemorrhage in rats.
The glial cells play an important role in pathophysiology of the intracerebral haemorrhage (ICH). Thus the attempt at evaluating the possible influence of the propofol on the reactivity of astro- and microglial cells in the course of ICH was performed. 50 rats were divided into two groups depending on the applied anaesthesia. All animals were generally anaesthetized with fentanyl, dehydrobenzperidol and midazolam. No additional agents were given to the animals of the control group (group I). In the experimental group (group II), the animals received additionally intraperitoneally propofol in a dose of 50 mg/kg every thirty minutes. ICH was produced through infusion of the blood into the striatum. The astrocytic and microglial cells population was assessed on the 1, 3, 7, 14 and 21 days after producing a haematoma using antibodies anti-GFAP and OX42. The stereological analysis was applied to estimate the numerical density of immunoreactive cells and the distribution of their types. On the 14th and 21st days of observation the density of GFAP-immunoreactivity (ir) cells was significantly higher in group II than that in group I. There were no differences in percentage distribution of GFAP-ir astrocytes between group I and group II. On the 3rd, 14th and 21st days of observation the density of OX42-ir cells was higher in group II in comparison with group I. For the 7th, and 21st days of survival the percentage of the ameboid form of OX42-ir cells was significantly lower in group I than that in group II. The administration of propofol during anaesthesia in the animals with ICH has evoked an increase of the activation of the astro- and microglial cells.
Karwacki
Zbigniew
Z
Department of Neuroanesthesiology, Medical University of Gdańsk, 7 Debinki St, 80-211 Gdańsk, Poland. zkarw@amg.gda.pl
Kowiański
Przemysław
P
Dziewiatowski
Jerzy
J
Domaradzka-Pytel
Beata
B
Ludkiewicz
Beata
B
Wójcik
Sławomir
S
Narkiewicz
Olgierd
O
Moryś
Janusz
J
D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000601:surgery*; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D015742:Propofol / Q000494:pharmacology*; D051381:Rats
D018686:Anesthetics, Intravenous; D015742:Propofol