Identification of steviol glucuronide in human urine.
Stevioside (250 mg capsules) was given three times daily to 10 healthy subjects. Steviol glucuronide (steviol 19-O-beta-D-glucopyranosiduronic acid; MM, 494.58; melting point, 198-199 degrees C) was characterized in the 24 h urine as the only excretion product of oral stevioside by MS, NMR, IR, and UV spectroscopy. This is the first report on the unambiguous identification of steviol glucuronide in human urine.
Geuns
Jan M C
JM
Laboratory of Functional Biology, KULeuven, Kasteelpark Arenberg 31, B-3001 Leuven, Belgium. Jan.Geuns@bio.kuleuven.be
Buyse
Johan
J
Vankeirsbilck
Annelies
A
Temme
Elisabeth H M
EH
Compernolle
Frans
F
Toppet
Suzanne
S
D045786:Diterpenes, Kaurane / Q000008:administration & dosage / Q000652:urine*; D005960:Glucosides / Q000008:administration & dosage; D020719:Glucuronides / Q000652:urine*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D045786:Diterpenes, Kaurane; D005960:Glucosides; D020719:Glucuronides; C012043:stevioside; C012042:steviol