Immunohistochemical identification method of tumour cells in the S phase of mitotic cycle and its usefulness in diagnostics of mammary gland adenocarcinomas in bitches.
The studies aimed at identification of neoplastic cells at the S phase of mitotic cycle in mammary gland adenocarcinomas of bitches. The material was sampled from bitches of various races, aging 6 to 12 years, in which the mammary gland tumours developed spontaneously. The tumours were verified histopathologically and, then, immunohistochemical reactions were performed in order to detect cells which had incorporated BrdU (bromodeoxyuridine), contained Ki-67 or PCNA antigen. The histological preparations were photographed and obtained pictures were subjected to computer-assisted image analysis using Axiophot microscope (Carl Zeiss) coupled to a computer and the Multi-ScaneBase V 8.08 software, working under Windows. Fifty percent of sections from mammary gland adenocarcinomas demonstrated BrdU labelling index of 4-5%, 40% of 1-3%, while in the remaining 10% of examined tumours no BrdU incorporation could be demonstrated. No evident relationship could be detected between the presence of BrdU incorporation and Ki-67 or PCNA antigen presence but a significant correlation was demonstrated between the expression of Ki-67 and PCNA.
Department of Pathological Anatomy, Pathophysiology, Microbiology and Forensic Veterinary Medicine, Faculty of Veterinary Medicine, Agricultural Academy, ul. Norwida 31, 50-375 Wrocław, Poland. martin75@ozi.ar.wroc.pl
M
M
Nowak
J A
JA
Madej
P
P
Dziegiel
H
H
Kanzawa
D000230:Adenocarcinoma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division; D004283:Dog Diseases / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D015674:Mammary Neoplasms, Animal / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D008938:Mitosis / Q000502:physiology; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*; D016196:S Phase / Q000502:physiology
D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D001973:Bromodeoxyuridine