Isolation of human antibody repertoires with preservation of the natural heavy and light chain pairing.
The humoral immune system in higher vertebrates is unique in its ability to generate highly diverse antibody responses against most pathogens as well as against certain malignancies. Several technologies have been developed to exploit this vast source of potentially therapeutic antibodies, including hybridoma technology, phage display and yeast display. Here, we present a novel, high-throughput technology (the Symplex Technology) for rapid direct cloning and identification of human antigen-specific high-affinity antibodies from single antibody-producing cells of immune individuals. The utility of the technology was demonstrated by isolation of diverse sets of unique high-affinity antibodies against tetanus toxoid and influenza virus from immunized volunteers. Hence, the Symplex Technology is a new method for the rapid isolation of high-affinity antibodies directly from humans.
Symphogen A/S, Elektrovej, Building 375, DK-2800 Lyngby, Denmark.
Per-Johan
PJ
Meijer
Peter S
PS
Andersen
Margit
M
Haahr Hansen
Lucilla
L
Steinaa
Allan
A
Jensen
Johan
J
Lantto
Martin B
MB
Oleksiewicz
Kaja
K
Tengbjerg
Tine R
TR
Poulsen
Vincent W
VW
Coljee
Søren
S
Bregenholt
John S
JS
Haurum
Lars S
LS
Nielsen
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification* / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D019151:Peptide Library; D010802:Phylogeny; D013744:Tetanus Toxin / Q000276:immunology
D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D019151:Peptide Library; D013744:Tetanus Toxin