Assessment of analytical reproducibility of 1H NMR spectroscopy based metabonomics for large-scale epidemiological research: the INTERMAP Study.
Large-scale population phenotyping for molecular epidemiological studies is subject to all the usual criteria of analytical chemistry. As part of a major phenotyping investigation we have used high-resolution 1H NMR spectroscopy to characterize 24-h urine specimens obtained from population samples in Aito Town, Japan (n = 259), Chicago, IL (n = 315), and Guangxi, China (n = 278). We have investigated analytical reproducibility, urine specimen storage procedures, interinstrument variability, and split specimen detection. Our data show that the multivariate analytical reproducibility of the NMR screening platform was >98% and that most classification errors were due to urine specimen handling inhomogeneity. Differences in metabolite profiles were then assessed for Aito Town, Chicago, and Guangxi population samples; novel combinations of biomarkers were detected that separated the population samples. These cross-population differences in urinary metabolites could be related to genetic, dietary, and gut microbial factors.
Dumas
Marc-Emmanuel
ME
Biological Chemistry, Biomedical Sciences Division, Imperial College London, Sir Alexander Fleming Building, South Kensington, London UK. m.dumas@imperial.ac.uk
Maibaum
Elaine C
EC
Teague
Claire
C
Ueshima
Hirotsugu
H
Zhou
Beifan
B
Lindon
John C
JC
Nicholson
Jeremy K
JK
Stamler
Jeremiah
J
Elliott
Paul
P
Chan
Queenie
Q
Holmes
Elaine
E
D015415:Biomarkers / Q000652:urine; D002681:China; D002947:Cities; D003430:Cross-Sectional Studies; D005819:Genetic Markers / Q000235:genetics / Q000502:physiology; D006801:Humans; D007564:Japan; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards; D015999:Multivariate Analysis; D010641:Phenotype; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods* / Q000592:standards; D013997:Time Factors; D014481:United States; D016482:Urinalysis
D015415:Biomarkers; D005819:Genetic Markers