[Experimental study on acellular bovine pericardium guided bone regeneration material].
OBJECTIVE
To choose the best procedure on preparation of acellular bovine pericardium (ABP) guided bone regeneration (GBR) material.


METHODS
The BP was decellularized with 0. 25% Trypsin + 0.5% Triton X-100. The acellular bovine pericardiums (ABPs) were treated with phosphate-buffered saline(PBS) (group A), 95% glycerol (group B), EDAC (group C), and EDAC and 95% glycerol (group D) respectively. The treated ABPs were implanted subcutaneously in the back of SD rats respectively at random and no material was implanted as control. Seven rats were sacrificed at 2 weeks, twelve at 4 weeks, twelve at 8 weeks, seven at 16 weeks. Local reaction was studied grossly. The amount of antigen presenting cell (APC) and the percentage of ABP degeneration were reckoned by images analysis system.


RESULTS
The ABPs were replaced by fibroblasts completely in group A at 8 weeks, in group C at 16 weeks, but only less than 50% till 16 weeks in groups B and D. In all groups, the depth of surrounding fibres attenuated time-dependently. The APC amount of the groups B and D was higher than that of the control group, and the ABP of the groups B and D degraded partly at 16 weeks.


CONCLUSIONS
The ABP treated with EDAC can be replaced by the surrounding tissues and has good biocompatibility.
Department of Stomatology, Xiangya Hospital,Central South University, Changsha Hunan 410000, P R China.
Wenbin
W
Zhang
Hanjiang
H
Wu
Benzhan
B
Yao
Guangwei
G
Hu
Yiqun
Y
Zhou
D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration; D002417:Cattle; D008297:Male; D010496:Pericardium / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering
D001672:Biocompatible Materials