Constructing a tissue-engineered ureter using a decellularized matrix with cultured uroepithelial cells and bone marrow-derived mononuclear cells.
This study investigated the efficacy of the ureteral decellularized matrix (UDM) as a scaffold material for a tissue-engineered ureter, and the effect of bone marrow-derived mononuclear cells (BM-MNC) on the neovascularization of the scaffold. Canine ureters were treated with deoxycholic acid to remove all cells. Uroepithelial cells (UEC) were obtained from canine bladders, cultured, and then seeded onto the inner surface of the UDM before transplantation into the subcutaneous space of nude mice or the omentum of nude rats. The cultured UECs began showing vacuolar degeneration 3 days after transplantation and gradually disappeared thereafter. To facilitate neovascularization in the implant, BM-MNCs were seeded around the UDM before transplantation. This facilitated the survival of the UECs, which formed three to five cellular layers after 14 days. The mean microvessel density was significantly increased in tissues seeded with BM-MNCs. However, cell-tracking experiments revealed that the increased number of capillaries in the experimental group was not due to the direct differentiation of transplanted endothelial progenitor cells. Our results demonstrate that the UDM is a useful scaffold for a tissue-engineered ureter, especially when seeded with BM-MNCs to enhance angiogenesis.
Matsunuma
Hiroshi
H
Department of Urology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Kagami
Hideaki
H
Narita
Yuji
Y
Hata
Ken-Ichiro
K
Ono
Yoshonari
Y
Ohshima
Shinichi
S
Ueda
Minoru
M
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology / Q000637:transplantation; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000637:transplantation; D051379:Mice; D008819:Mice, Nude; D018919:Neovascularization, Physiologic; D051381:Rats; D011923:Rats, Nude; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous; D014513:Ureter / Q000098:blood supply / Q000166:cytology; D019459:Urothelium / Q000166:cytology