Antioxidant enzymes activities and protein damage in rat brain of both sexes.
The theory of free radicals and accumulation of damages suggests that the reactive species of oxygen play a key role in the context of aging. Thus, for the best understanding of the aging process, the study of antioxidant defenses has to be considered as part of gerontology. The present work evaluated the enzymatic activity of the enzymes catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), and measured the amount of oxidative damage in proteins (carbonyl groups) in brains of rats of both sexes in the ages of 3-, 6-, 12- and 20-months. The results suggest that the patterns of activity and accumulation of damages can be sex-specific and related to the cycle of reproductive life of the organism.
Laboratório de Estresse Oxidativo, Departamento de Biofísica-IB-UFRGS, Porto Alegre, Brazil.
Guilherme
G
Ehrenbrink
Fernanda Schäfer
FS
Hakenhaar
Tiago Boeira
TB
Salomon
Antonella Pilla
AP
Petrucci
Marcia Rodrigues
MR
Sandri
Mara Silveira
MS
Benfato
D000375:Aging / Q000502:physiology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001921:Brain / Q000201:enzymology* / Q000378:metabolism; D002374:Catalase / Q000032:analysis; D005260:Female; D005979:Glutathione Peroxidase / Q000032:analysis; D008297:Male; D018384:Oxidative Stress; D050050:Protein Carbonylation / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000502:physiology*; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000032:analysis
D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase