Single-step DNA immobilization on antifouling self-assembled monolayers covalently bound to silicon (111).
Hydrosilylation of alkenes with epoxide-terminated tri(ethylene oxide) moieties on Si-H surfaces yields homogeneous monolayers for the efficient coupling of biomolecules. The wetting properties of the epoxide-functionalized surface allow for the spotting of solutions of biomolecules, making the surface amenable to microarraying. Immobilization of thiolated DNA was achieved in a single step to fabricate biorecognition interfaces showing the hybridization of complementary DNA at low concentrations and negligible binding of noncomplementary DNA.
Böcking
Till
T
School of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia.
Kilian
Kristopher A
KA
Gaus
Katharina
K
Gooding
J Justin
JJ
D000475:Alkenes / Q000737:chemistry; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010087:Oxides / Q000737:chemistry; D012666:Semiconductors; D012821:Silanes / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties
D000475:Alkenes; D004852:Epoxy Compounds; D010087:Oxides; D012821:Silanes; D013438:Sulfhydryl Compounds; D006859:Hydrogen; D004247:DNA; D012825:Silicon