Crystallization and preliminary X-ray analysis of the C-terminal RNase III domain of human Dicer.
Human Dicer protein contains two RNase III domains (RNase IIIa and RNase IIIb) which are involved in the production of short interfering RNAs (siRNAs). The C-terminal RNase III domain (RNase IIIb) of human Dicer was expressed, purified and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group C222(1), with unit-cell parameters a = 88.6, b = 199.7, c = 119.6 angstroms, and diffracted X-rays to 2.0 angstroms resolution. The asymmetric unit contained three molecules of the RNase IIIb and the solvent content was 67%.
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Daijiro
D
Takeshita
Shuhei
S
Zenno
Woo Cheol
WC
Lee
Koji
K
Nagata
Kaoru
K
Saigo
Masaru
M
Tanokura
D003001:Cloning, Molecular; D003460:Crystallization; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D043244:Ribonuclease III / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014961:X-Ray Diffraction
D010446:Peptide Fragments; D011994:Recombinant Proteins; D043244:Ribonuclease III