Lachesis muta (Viperidae) cDNAs reveal diverging pit viper molecules and scaffolds typical of cobra (Elapidae) venoms: implications for snake toxin repertoire evolution.
Efforts to describe toxins from the two major families of venomous snakes (Viperidae and Elapidae) usually reveal proteins belonging to few structural types, particular of each family. Here we carried on an effort to determine uncommon cDNAs that represent possible new toxins from Lachesis muta (Viperidae). In addition to nine classes of typical toxins, atypical molecules never observed in the hundreds of Viperidae snakes studied so far are highly expressed: a diverging C-type lectin that is related to Viperidae toxins but appears to be independently originated; an ohanin-like toxin, which would be the third member of the most recently described class of Elapidae toxins, related to human butyrophilin and B30.2 proteins; and a 3FTx-like toxin, a new member of the widely studied three-finger family of proteins, which includes major Elapidae neurotoxins and CD59 antigen. The presence of these common and uncommon molecules suggests that the repertoire of toxins could be more conserved between families than has been considered, and their features indicate a dynamic process of venom evolution through molecular mechanisms, such as multiple recruitments of important scaffolds and domain exchange between paralogs, always keeping a minimalist nature in most toxin structures in opposition to their nontoxin counterparts.
Junqueira-de-Azevedo
Inácio L M
IL
Centro de Biotechnologia, Instituto Butantan, São Paulo, Brazil. ijuncaze@butantan.gov.br
Ching
Ana T C
AT
Carvalho
Eneas
E
Faria
Fernanda
F
Nishiyama
Milton Y
MY
Ho
Paulo L
PL
Diniz
Marcelo R V
MR
D000595:Amino Acid Sequence; D000818:Animals; D003435:Crotalid Venoms / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D004546:Elapid Venoms / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D017815:Elapidae / Q000145:classification / Q000235:genetics*; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D017819:Viperidae / Q000145:classification / Q000235:genetics*
D003435:Crotalid Venoms; D018076:DNA, Complementary; D004546:Elapid Venoms; C072644:Lachesis venom