Oxidative stress in peripheral blood mononuclear cells from patients with Parkinson's disease: negative correlation with levodopa dosage.
Oxidative stress, resulting from the imbalance between reactive oxygen species (ROS) formation and antioxidant defenses, plays a major role in the pathogenesis of Parkinson's disease (PD). However, the contribution of levodopa (LD) therapy to oxidative damage is still debated. We investigated oxidative stress in peripheral blood mononuclear cells (PBMCs) from LD-treated PD patients and healthy subjects. Increased ROS production associated with unaltered glutathione reductase activity was detected in PBMC from PD patients. LD daily dosage appeared to be inversely correlated with ROS levels and positively associated with GR activity, suggesting a protective role for LD on PBMCs redox status. Our data support the view of systemic oxidative stress involvement in PD and give further rationale for using PBMCs as an easily accessible ex-vivo dopaminergic model for exploring the biological effects of LD therapy.
Laboratory of Neurobiology, Department of Neuroscience and Biomedical Technologies, University of Milan-Bicocca, Italy.
Alessandro
A
Prigione
Barbara
B
Begni
Alessio
A
Galbussera
Simone
S
Beretta
Laura
L
Brighina
Rosanna
R
Garofalo
Simona
S
Andreoni
Roberto
R
Piolti
Carlo
C
Ferrarese
D000368:Aged; D000978:Antiparkinson Agents / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005980:Glutathione Reductase / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D007980:Levodopa / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D010300:Parkinson Disease / Q000188:drug therapy*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
D000978:Antiparkinson Agents; D017382:Reactive Oxygen Species; D007980:Levodopa; D005980:Glutathione Reductase