Forensic mass screening using mtDNA.
At the forensic autopsy of a sexual murder victim, some trace hairs, possibly belonging to the perpetrator, were saved. Initially, the analysis of a pubic hair shaft only revealed the presence of the mitochondrial (mt) DNA haplotype profile consisting of the (CA)(6) allele and the complete hypervariable region 1 (HV1) and 2 (HV2) sequence. Later, typing of some further telogene trace hairs, which had been stored for several years, yielded a nuclear short tandem repeat (STR) profile. We used both the mtDNA haplotype and the STR profile to start a DNA mass screening project involving 2,335 male citizens of the relevant communities. MtDNA screening was carried out by using the CA repeat amplification in combination with an SNP typing procedure based on the restriction site analysis of amplified d-loop sequences. The aim of our paper is to put mass screening with mtDNA up for discussion.
Szibor
Reinhard
R
Institut für Rechtsmedizin, Otto-von-Guericke-Universität Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, Germany. reinhard.szibor@medizin.uni-magdeburg.de
Plate
Ines
I
Schmitter
Herrmann
H
Wittig
Holger
H
Krause
Dieter
D
D000483:Alleles; D002648:Child; D022801:Complementarity Determining Regions / Q000235:genetics; D016172:DNA Fingerprinting; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D005820:Genetic Testing; D006197:Hair; D006239:Haplotypes; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011902:Rape; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences
D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial