Toward a physical map of the genome of the nematode Caenorhabditis elegans.
A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described. The technique is being applied to fragments from the nematode Caenorhabditis elegans (i) to facilitate cross-indexing of clones emanating from different laboratories and (ii) to construct a physical map of the genome. Eight hundred sixty clusters of clones, from 35 to 350 kilobases long and totaling about 60% of the genome, have been characterized.
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England.
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Coulson
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Sulston
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Brenner
J
J
Karn