Characterisation and foaming properties of hydrolysates derived from rapeseed isolate.
Two hydrolysis methods used to obtain rapeseed isolate derivates were compared: chemical hydrolysis performed under alkaline conditions and pepsic proteolysis performed under acidic conditions. The mean molecular weights obtained for the hydrolysates varied from 26 to 2.5 kDa, depending on the level of hydrolysis. Further characterisation showed that, at the same level of hydrolysis, the chemical hydrolysates differed by their charges and hydrophobicity from those derived from enzymatic digestion. Analysis of the foaming properties showed, for both cases, that a limited degree of hydrolysis, around 3%, was sufficient to optimise the foaming properties of the isolate despite the different physicochemical properties of the peptides generated. The study of foaming properties at basic, neutral and acidic pHs showed that the hydrolysate solutions yielded dense foams which drained slowly and which maintained a very stable volume under the three pH conditions tested.
INRA, Unité de Recherche sur les Protéines Végétales et leurs Interactions, BP 71627, 44316 Nantes Cedex 3, France. larre@nantes.inra.fr
C
C
Larré
W
W
Mulder
R
R
Sánchez-Vioque
J
J
Lazko
S
S
Bérot
J
J
Guéguen
Y
Y
Popineau
D029741:Brassica rapa / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry; D011492:Protein Hydrolysates / Q000737:chemistry*; D012995:Solubility; D012996:Solutions / Q000737:chemistry; D013997:Time Factors
D010455:Peptides; D011492:Protein Hydrolysates; D012996:Solutions