Delineation of Borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of Borrelia spielmanii sp. nov.
To evaluate multilocus sequence analysis (MLSA) for taxonomic purposes in the delineation of species within Borrelia burgdorferi sensu lato, seven relevant loci of various strains for which extensive DNA-DNA reassociation data were available were sequenced. MLSA delineation proved to be fully concordant with conventional methods. Our analysis confirmed the delineation of a novel species, Borrelia spielmanii sp. nov., previously known as 'Borrelia spielmani' Richter et al. 2004, with strain PC-Eq17N5T (=DSM 16813T = CIP 108855T) as the type strain.
Richter
Dania
D
Charité Universitätsmedizin Berlin, 12249 Berlin, Germany.
Postic
Danièle
D
Institut Pasteur, 75724 Paris, France.
Sertour
Natacha
N
Institut Pasteur, 75724 Paris, France.
Livey
Ian
I
Baxter Vaccine AG, A-2304 Orth/Donau, Austria.
Matuschka
Franz-Rainer
FR
Charité Universitätsmedizin Berlin, 12249 Berlin, Germany.
Baranton
Guy
G
Institut Pasteur, 75724 Paris, France.
D015373:Bacterial Typing Techniques; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry*; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
D004269:DNA, Bacterial