Maternally derived recombinant human anti-hantavirus monoclonal antibodies are transferred to mouse offspring during lactation and neutralize virus in vitro.
Transgenic mice expressing a recombinant human monoclonal antibody (rHMAb) against hantavirus were generated. These mice could be used as models to explore the possibilities of producing rHMAbs for therapeutic purposes. The highest concentration of the rHMAb in the milk of the transgenic females was 6.6 mg/ml. The rHMAb was also detected in the sera of pups fed by the transgenic females. Both the rHMAbs in the milk of transgenic mice and those in the sera of suckling pups were found to be active against hantaviruses, although the light chain of the antibody absorbed by the pups was modified by N-linked glycosylation.
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China.
Shuyang
S
Yu
Mifang
M
Liang
Baoliang
B
Fan
Hongtao
H
Xu
Chuan
C
Li
Quanfu
Q
Zhang
Dexin
D
Li
Bo
B
Tang
Shijie
S
Li
Yunping
Y
Dai
Meili
M
Wang
Min
M
Zheng
Bingxue
B
Yan
Qinghong
Q
Zhu
Ning
N
Li
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006476:Orthohantavirus / Q000276:immunology*; D007112:Immunity, Maternally-Acquired; D007774:Lactation; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D011994:Recombinant Proteins