[Effect of realgar on the gene expression profile of multiple myeloma cell line RPMI 8226].
OBJECTIVE
To explore the effect of realgar on the gene expression profiles of multiple myeloma cell line RPMI 8226 by apply cDNA microarray.


METHODS
The gene expression of RPMI 8226 cells before and after 48 hours of realgar treatment was determined with a cDNA microarray representing 4096 human genes.


RESULTS
At the mRNA level, 164 genes were differentially altered; 53 genes were up-regulated; and 111 genes were down-regulated.


CONCLUSIONS
The realgar treatment to RPMI 8226 cell line may induce a number of gene changes. Many genes may be involved in the pathogenesis of multiple myeloma. BTG1, ALK1, and TXNIP genes may play an important role in the apoptosis and differentiation of RPMI 8226 cells.
Department of Hematology, First Hospital of Xi' an Jiaotong University, Xi' an, Shaanxi Province 710061, China. swallow3956@sina.com
Meng-chang
MC
Wang
Shan-xi
SX
Liu
Peng-bo
PB
Liu
D001152:Arsenicals / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006801:Humans; D009101:Multiple Myeloma / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D013440:Sulfides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001152:Arsenicals; D013440:Sulfides; C058317:arsenic disulfide