In vitro effects of epristeride on sperm in rats, dogs and man.
The study was conducted to evaluate in vitro effects of epristeride on sperm in rats, beagle dogs and man. Semen samples were divided into 4 groups and treated with vehicle and epristeride. Motility and motile rate of sperm were videotaped and analyzed with CASA system after 1 h and 2 h incubation periods. Percentage of motile sperm (MOT) of rat sperm decreased after the treatment with epristeride (final concentrations were 0.6, 6 and 60 micromol/L) for 1 h, and MOT of rat sperm treated with middle dose and high dose levels of epristeride also decreased after 2 h, while MOT of dog sperm that treated with three dose levels of epristeride decreased after 2 h. Amplitude of lateral head displacement (ALH) and MOT of human sperm decreased after 2 h with 4.8 micromol/L epristeride treatment. Curvilinear velocity (VCL) and straight-line velocity (VSL) of rat sperm and human sperm changed after 2 h, but there were no significant differences. Therefore, epristeride had a toxic effect on sperm, and the effect varied in different species.
Wu
J H
JH
National Evaluation Centre for the Toxicology of Fertility Regulating Drugs, Shanghai Institute of Planned Parenthood Research, Shanghai, China. wwjh_731@yahoo.com.cn
Sun
Z Y
ZY
Cao
L
L
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000633:toxicity*; D000730:Androstadienes / Q000633:toxicity*; D000818:Animals; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
D000730:Androstadienes; D004791:Enzyme Inhibitors; C072547:epristeride; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase