Leaf epicuticular wax chemicals of the Japanese knotweed Fallopia japonica as oviposition stimulants for Ostrinia latipennis.
Extraction, fractionation, and gas chromatography-mass spectrometry analyses guided by bioassays have shown that n-alkanes and free fatty acids in leaf epicuticular wax of the Japanese knotweed Fallopia (Reynoutria) japonica stimulate oviposition in the Far-Eastern knotweed borer, Ostrinia latipennis (Lepidoptera: Crambidae). n-Alkanes made up 48.1% of the total amount of epicuticular wax, and their carbon chain length was in the C(16)-C(33) range, with n-nonacosane (n-C(29)) most abundant, followed by n-C(27), n-C(25), and n-C(31). Free fatty acids with C(9)-C(22) accounted for 22.3%, and hexadecanoic acid was predominant. A mixture of authentic n-alkanes and fatty acids of the composition found in the epicuticular wax, a mixture of n-alkanes, and a mixture of fatty acids significantly enhanced oviposition. Thus, it was demonstrated that both n-alkanes and free fatty acids in leaf epicuticular wax of F. japonica are naturally occurring oviposition stimulants for O. latipennis.
Li
Guoqing
G
Laboratory of Applied Entomology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. liguoqing001234@yahoo.com.cn
Ishikawa
Yukio
Y
D000473:Alkanes / Q000737:chemistry; D000818:Animals; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D045468:Fallopia japonica / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D007915:Lepidoptera / Q000502:physiology*; D013058:Mass Spectrometry; D010058:Oviposition / Q000502:physiology*; D010063:Ovum / Q000502:physiology; D019441:Plant Epidermis / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D014885:Waxes / Q000032:analysis / Q000737:chemistry*
D000473:Alkanes; D005227:Fatty Acids; D014885:Waxes