Regulation of transduction efficiency by pegylation of baculovirus vector in vitro and in vivo.
In this study, poly(ethylene glycol) (PEG) was coupled to baculovirus to regulate transduction efficiency of baculovirus in vitro and in vivo. The degree of pegylation in virions was measured by the loss of free amines via a fluorescamine-based assay. The efficiency of green fluorescent protein (GFP) expression was used to monitor transduction efficiency. As the results, the transduction efficiency in pegylated baculovirus was decreased with an increase of pegylation in baculovirus in vitro and in vivo. Interestingly, the transduction efficiency of the pegylated baculovirus for the lung and brain was increased compared with baculovirus itself possibly owing to increased stability of baculovirus by pegylation.
School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea.
You-Kyoung
YK
Kim
In-Kyu
IK
Park
Hu-Lin
HL
Jiang
Jae-Young
JY
Choi
Yeon-Ho
YH
Je
Hua
H
Jin
Hyun-Woo
HW
Kim
Myung-Haing
MH
Cho
Chong-Su
CS
Cho
D000818:Animals; D016367:Baculoviridae / Q000737:chemistry / Q000235:genetics*; D002460:Cell Line; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics* / Q000493:pharmacokinetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism / Q000493:pharmacokinetics; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000493:pharmacokinetics; D014018:Tissue Distribution / Q000187:drug effects; D014162:Transfection / Q000379:methods*
D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols