Multifunctional polymeric nanoparticles from diverse bioactive agents.
We present a rational approach for assembling diverse bioactive agents, such as DNA, proteins, and drug molecules, into core-shell multifunctional polymeric nanoparticles (PNPs) that can be internalized in human breast cancer cells. Using ring-opening metathesis polymerization (ROMP), block copolymers containing small-molecule drug segments (>50% w/w) and tosylated hexaethylene glycol segments were prepared and assembled into PNPs that allowed for the surface conjugation of single-stranded DNA sequences and/or tumor-targeting antibodies. The resulting antibody-functionalized particles were readily uptaken by breast cancer cells that overexpressed the corresponding antigens.
Bertin
Paul A
PA
Department of Chemistry, Northwestern University, Evanston, Illinois 60208-3113, USA.
Gibbs
Julianne M
JM
Shen
Clifton Kwang-Fu
CK
Thaxton
C Shad
CS
Russin
William A
WA
Mirkin
Chad A
CA
Nguyen
Sonbinh T
ST
D000912:Antibodies, Neoplasm / Q000276:immunology / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D001643:Bridged Bicyclo Compounds / Q000737:chemistry* / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D004277:DNA, Single-Stranded / Q000737:chemistry*; D005026:Ethylene Glycols / Q000737:chemistry* / Q000493:pharmacokinetics; D019793:Fluorescein / Q000493:pharmacokinetics; D006536:Heptanes / Q000737:chemistry* / Q000493:pharmacokinetics; D006801:Humans; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D009841:Oligonucleotides / Q000235:genetics / Q000493:pharmacokinetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet; D014105:Tosyl Compounds / Q000737:chemistry* / Q000493:pharmacokinetics
D000912:Antibodies, Neoplasm; D001643:Bridged Bicyclo Compounds; D004277:DNA, Single-Stranded; D005026:Ethylene Glycols; D019793:Fluorescein; D006536:Heptanes; D009841:Oligonucleotides; D019253:Proto-Oncogene Proteins c-bcl-2; D018719:Erb-b2 Receptor Tyrosine Kinases; D014105:Tosyl Compounds; C504926:(2.2.1)bicyclohept-2-ene; C077067:hexaethylene glycol