Analysis of neolignans compounds of Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck by HPLC.
A high performance liquid chromatographic (HPLC) method was developed and validated for quantitative determination of neolignans in extracts of Piper regnellii var. pallescens. The analysis were carried out on a Metasil ODS column (150 mm x 4.6 mm, 5 microm) at 30 degrees C, using as mobile phase acetonitrile-water (60:40, v/v) containing 2% acetic acid. The flow rate was 1.0 ml/min and the detection was at 280 nm. The validation using conocarpan as standard demonstrated that the method presents linearity (linear correlation coefficient=0.9991), precision (relative standard deviation <5%) and accuracy (mean recovery=104.55%) in the concentration range 31.25-500 microg/ml. The limit of detection (LOD) was 1.68 microg/ml and the limit of quantitation was 5.60 microg/ml. This method allowed the identification and quantification of conocarpan, eupomatenoid-5 and eupomatenoid-6 in the hydroethanolic extracts obtained from the leaves, stems and roots by maceration process. All the extracts showed the same chromatographic profile, being that the extract of the roots presented the highest concentration of neolignans.
Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Farmácia e Farmacologia, Universidade Estadual deMaringá, Avenida Colombo 5790, Maringá PR 87020-900, Brazil.
Daniele Fernanda
DF
Felipe
Benedito Prado Dias
BP
Filho
Celso Vataru
CV
Nakamura
Selma Lucy
SL
Franco
Diógenes Aparício Garcia
DA
Cortez
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017705:Lignans / Q000032:analysis* / Q000302:isolation & purification; D031701:Piper; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results
D017705:Lignans; D010936:Plant Extracts