Castellaniella gen. nov., to accommodate the phylogenetic lineage of Alcaligenes defragrans, and proposal of Castellaniella defragrans gen. nov., comb. nov. and Castellaniella denitrificans sp. nov.
Comparative 16S rRNA gene sequence analysis indicates that two distinct sublineages exist within the genus Alcaligenes: the Alcaligenes faecalis lineage, comprising Alcaligenes aquatilis and A. faecalis (with the three subspecies A. faecalis subsp. faecalis, A. faecalis subsp. parafaecalis and A. faecalis subsp. phenolicus), and the Alcaligenes defragrans lineage, comprising A. defragrans. This phylogenetic discrimination is supported by phenotypic and chemotaxonomic differences. It is proposed that the A. defragrans lineage constitutes a distinct genus, for which the name Castellaniella gen. nov. is proposed. The type strain for Castellaniella defragrans gen. nov., comb. nov. is 54PinT (=CCUG 39790T = CIP 105602T = DSM 12141T). Finally, on the basis of data from the literature and new DNA-DNA hybridization and phenotypic data, the novel species Castellaniella denitrificans sp. nov. (type strain NKNTAUT = DSM 11046T = CCUG 39541T) is proposed for two strains previously identified as strains of A. defragrans.
Kämpfer
P
P
Institut für Angewandte Mikrobiologie, Justus Liebig Universität, IFZ - Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
Denger
K
K
Department of Biology, The University of Konstanz, D-78457 Konstanz, Germany.
Cook
A M
AM
Department of Biology, The University of Konstanz, D-78457 Konstanz, Germany.
Lee
S-T
ST
Department of Biological Sciences, Korean Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejon, 305-701, Republic of Korea.
Jäckel
U
U
Institut für Angewandte Mikrobiologie, Justus Liebig Universität, IFZ - Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
Denner
E B M
EBM
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Veterinärplatz 1, A-1210 Wien, Austria.
Busse
H-J
HJ
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Veterinärplatz 1, A-1210 Wien, Austria.
D042401:Alcaligenaceae / Q000145:classification* / Q000302:isolation & purification; D000421:Alcaligenes / Q000145:classification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics
D012336:RNA, Ribosomal, 16S