[Isolation and purification of an inhibitor on platelet aggregation from Ixodes sinensis].
OBJECTIVE
To study the bioactive components in ticks which inhibit platelet aggregation, and to understand the molecular mechanism of tick-host interaction.


METHODS
Sephadex G-50 gel filtration and high performance liquid chromatography (HPLC) were used to purify the platelet aggregation inhibitor from lxodes sinensis. Its molecular weight and purity were checked by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Platelet-rich plasma (PRP) of rabbit was used to examine the function of platelet aggregation inhibitor.


RESULTS
A purified platelet aggregation inhibitor was identified from L. sinensis with a molecular weight of 8 065. It inhibited platelet aggregation induced by ADP with strong potency. The inhibition of platelet aggregation reached over 90% under a concentration of 10 microg/ml.


CONCLUSIONS
An inhibitor of platelet aggregation from L. sinensis was identified, which may play an important role for ticks to successfully get blood meal from their hosts.
Liu
Yong-hou
YH
Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
Xu
Chun-hua
CH
Liu
Zhi-gang
ZG
Liang
Jian-guo
JG
Lai
Ren
R
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D026863:Ixodidae / Q000737:chemistry*; D008970:Molecular Weight; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010975:Platelet Aggregation Inhibitors