High-resolution physical mapping and construction of a porcine contig spanning the intramuscular fat content QTL.
We previously mapped a locus for porcine intramuscular fat content (IMF) by linkage analysis to a 17.1-cM chromosome interval on Sus scrofa chromosome 7 (SSC7) flanked by microsatellite markers SW1083 and SW581. In this study, we identified 34 microsatellite markers and 14 STSs from the 17.1-cM IMF quantitative trait loci (QTL) region corresponding to HSA14q and aligned those loci using the INRA-University of Minnesota porcine radiation hybrid (IMpRH) panel. We then constructed a 5.2-Mb porcine bacterial artificial chromosome (BAC) contig of this region that was aligned using the RH panel. Finally, the IMF QTL was fine-mapped to 12.6 cM between SJ169 and MM70 at the 0.1% chromosome-wise significance level by genotyping the previously studied F2 resource family with 17 additional microsatellites. We also demonstrated that the SJ169-MM70 interval spans approximately 3.0 Mb and contains at least 12 genes: GALC, GPR65, KCNK10, SPATA7, PTPN21, FLJ11806, EML5, TTC8, CHES1, CAP2P1, CHORDC2P and C14orf143.
National Livestock Breeding Center, Nishigo, Fukushima 961-8511, Japan.
S
S
Sato
H
H
Hasebe
S
S
Sato
Y
Y
Asahi
T
T
Hayashi
E
E
Kobayashi
Y
Y
Sugimoto
D000273:Adipose Tissue / Q000033:anatomy & histology; D050154:Adiposity / Q000235:genetics*; D000818:Animals; D001947:Breeding; D022202:Chromosomes, Artificial, Bacterial; D020451:Contig Mapping; D008297:Male; D008460:Meat / Q000592:standards; D018895:Microsatellite Repeats; D040641:Quantitative Trait Loci; D021101:Radiation Hybrid Mapping; D013552:Swine / Q000033:anatomy & histology / Q000235:genetics*