[Expression of HOXB4 in cord blood progenitor cells expanded in vitro].
HOXB4, a member of homeobox gene family, is closely related to the self-renewing and proliferative ability of primitive hematopoietic stem/progenitor cells (PHSC/PHPC). This study was aimed to investigate the self-renewing level of cord blood progenitor cells (CBPC) expanded in vitro. The HOXB4 expression at mRNA level was assayed by using real time RT-PCR. The results indicated that as culture prolonged, the total cells, CD34(+) cells greatly increased, however the HOXB4 expression gradually declined, even down to undetectable level similar to that of mature lymphocytes. Meanwhile, it was shown that CD34(+) cells co-cultured with bone marrow mesenchymal stem cells (BM-MSC) could abate the decline of HOXB4 expression. It is concluded that the self-renewing potential of CD34(+) cells gradually decreased during expansion in vitro, co-culture with BM-MSC was helpful to CD34(+) cell expansion and slowed the loss trend of its self-renewal.
Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Yu-Hong
YH
Tang
Xiao-Ming
XM
Fei
Wen-Yi
WY
Shen
Kou-Rong
KR
Miao
Yu-Gui
YG
Cui
Cheng-Ya
CY
Wang
D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology*; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
D018952:Antigens, CD34; C090460:HOXB4 protein, human; D018398:Homeodomain Proteins; D014157:Transcription Factors