Congo red interaction with alpha-proteins.
The ability of Congo red to form complexes with alpha-proteins, human growth hormone and human interferon-alpha2b, was found by absorption difference spectroscopy. A human growth hormone-Congo red complex was isolated by gel-permeation chromatography, and its visible absorption spectrum was registered in comparison to free dye. The ability of Congo red to induce dimerization of human growth hormone was demonstrated using chemical cross-linking agents 1,3,5-triacryloyl-hexahydro-s-triazine and ethylene glycol bis(succinimidylsuccinate).
Department of Chemistry and Bioengineering, Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Vilnius, Lithuania. sjolanta@fm.vtu.lt
Jolanta
J
Sereikaite
Vladas-Algirdas
VA
Bumelis
D001703:Biophysics / Q000379:methods; D002850:Chromatography, Gel; D003224:Congo Red / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D019281:Dimerization; D005456:Fluorescent Dyes / Q000494:pharmacology*; D019382:Human Growth Hormone / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000737:chemistry*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D011994:Recombinant Proteins; D013053:Spectrophotometry; D013388:Succinimides / Q000737:chemistry; D014227:Triazines / Q000494:pharmacology
D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D013388:Succinimides; D014227:Triazines; D019382:Human Growth Hormone; D003224:Congo Red; C028722:ethylene glycolylbis(succinimidyl succinate); C049485:1,3,5-triacryloylhexahydrotriazine