Detecting the cytogenetic effects in workers occupationally exposed to vincristine with four genetic tests.
To study the human genetic damage induced by vincristine (VCR), the cytogenetic effects in workers occupationally exposed to vincristine were studied with micronucleus (MN) test, comet assay, hypoxantinepho-guanine phosphoribosyl-transferase (hprt) gene mutation assay and T-cells receptor (TCR) gene mutation assay. Fresh peripheral blood samples were collected from the workers and controls. Fifteen workers from a plant producing antineoplastic drug (vincristine) and 15 controls were matched according to age, gender and smoking. The results of MN test showed that the mean micronuclei rate (MNR) and mean micronucleated cells rate (MCR) in 15 workers were 17.80+/-1.88 per thousand and 13.67+/-1.56 per thousand, respectively, which were significantly higher than those (3.73+/-0.80 per thousand and 3.13+/-0.59 per thousand) in controls (P<0.01). It was found in the comet assay that the mean tail length (MTL) of 15 workers and 15 controls were 1.72+/-0.15 microm and 0.71+/-0.01 microm, respectively, there was significant difference between workers and controls for MTL (P<0.05), but the difference between the mean tail moment (MTM, 0.29+/-0.03) of 15 workers and MTM (0.17+/-0.05) of 15 controls was not significant (P>0.05). The results of hprt gene mutation assay showed that the average mutation frequency of hprt (Mf-hprt) in workers was 1.03+/-0.02 per thousand, which was significantly higher than that (0.87+/-0.01 per thousand) in controls (P<0.05). Meanwhile, the results of TCR gene mutation assay indicated that Mfs-TCR of workers and controls were 2.52+/-0.34 x 10(-4) and 1.51+/-0.11 x 10(-4), respectively, there was a significant difference between workers and controls (P<0.01). It is found in the results of our study that the genetic damage is detectable in 15 workers occupationally exposed to vincristine.
Zhejiang University, Medical College, Institute of Occupational and Environmental Health, Hangzhou 310006, Zhejiang, PR China.
Deng
D
Hongping
Lou
L
Jianlin
Zhang
Z
Meibian
Wu
W
Wei
Jin
J
Lifen
Chen
C
Shijie
Zheng
Z
Wei
Wang
W
Baohong
He
H
Jiliang
D000328:Adult; D000972:Antineoplastic Agents, Phytogenic / Q000009:adverse effects*; D016022:Case-Control Studies; D020552:Comet Assay; D003582:Cytogenetics; D005260:Female; D019672:Genes, T-Cell Receptor; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009153:Mutagens / Q000009:adverse effects*; D009154:Mutation; D016273:Occupational Exposure; D014750:Vincristine / Q000009:adverse effects*
D000972:Antineoplastic Agents, Phytogenic; D009153:Mutagens; D014750:Vincristine; D007041:Hypoxanthine Phosphoribosyltransferase