Search for additional influenza virus to cell interactions.
Sialyl oligosaccharides have long been considered to be the sole receptors for influenza virus. However, according to [1] some viruses are able to grow in sialic-free MDCK cells. Here we attempted to reveal a possible second, non-sialic receptor, hypothesizing the involvement of additional carbohydrate lectin recognition in influenza virus reception process, first of all in situations when a lectin of the host cell could recognize the viral carbohydrate ligand. We tested the presence of galactose- and sialic acid-binding lectins, as well as mannoside- and sulfo-N-acetyllactosamine-recognizing properties of MDCK and Vero cells using polyacrylamide neoglycoconjugates and antibodies. MDCK cells bind galactoside probes stronger than Vero cells, whereas Vero cells bind preferentially sialoside, mannoside and various sulfo-oligosaccharide probes. The probing of viruses with the neoglycoconjugates revealed specific 6'-HSO (3) LacNAc (but not other sulfated oligosaccharides) binding property of A and B human strains. Affinity of 6'-HSO (3) LacNAc probe was comparable with affinity of 6'-SiaLac probe but the binding was not inhibited by the sialooligosaccharide.
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
E M
EM
Rapoport
L V
LV
Mochalova
H-J
HJ
Gabius
J
J
Romanova
N V
NV
Bovin
D000818:Animals; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004285:Dogs; D037161:Galectins / Q000378:metabolism; D006001:Glycoconjugates / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D015335:Molecular Probes / Q000378:metabolism; D008969:Molecular Sequence Data; D009975:Orthomyxoviridae / Q000378:metabolism* / Q000472:pathogenicity; D011991:Receptors, Virus / Q000378:metabolism; D014709:Vero Cells / Q000821:virology
D037161:Galectins; D006001:Glycoconjugates; D037102:Lectins; D015335:Molecular Probes; D011991:Receptors, Virus