Preferential transcription of cloned maize chloroplast DNA sequences by maize chloroplast RNA polymerase.
Zea mays chloroplast DNA-dependent RNA polymerase in vitro preferentially transcribes maize chloroplast DNA sequences incorporated in cloned chimeric bacterial plasmids. Preferential transcription is dependent on the presence of a 27.5-kilodalton polypeptide, the S factor, which has been purified from maize chloroplasts, and also on the template's being in the supercoiled form.
The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
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