Alpha-ketoglutarate modulates the circadian patterns of lipid peroxidation and antioxidant status during N-nitrosodiethylamine-induced hepatocarcinogenesis in rats.
The effect of alpha-ketoglutarate (alpha-KG) on circadian patterns of lipid peroxides and antioxidants in N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats has been studied. The circadian rhythm characteristics (acrophase, amplitude, and mesor) of thiobarbituric acid-reactive substances (TBARS), antioxidants, superoxide dismutase, catalase, glutathione peroxidase, and reduced glutathione were markedly altered in NDEA-treated rats. The delays in acrophase observed in NDEA-treated rats were brought back to near normal range by the administration of alpha-KG. An increase in mesor values of TBARS and a decrease in mesor values of antioxidants in NDEA-administered rats were reversed by alpha-KG administration. It can be concluded that alpha-KG exerts its chemopreventive effect by restoring antioxidants and their circadian rhythms.
Department of Biochemistry, Annamalai University, Annamalainagar, Tamil Nadu, India.
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Dakshayani
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Subramanian
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002374:Catalase / Q000032:analysis; D002940:Circadian Rhythm / Q000187:drug effects*; D004052:Diethylnitrosamine; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000032:analysis; D007656:Ketoglutaric Acids / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000032:analysis; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D000975:Antioxidants; D007656:Ketoglutaric Acids; D017392:Thiobarbituric Acid Reactive Substances; D004052:Diethylnitrosamine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione