The selective m1 muscarinic antagonist MT-7 blocks pilocarpine-induced striatal Fos expression.
Systemic administration of the nonselective muscarinic agonist pilocarpine induces pronounced striatal Fos expression. Intrastriatal injections of the mamba snake toxin MT-7, a highly selective and irreversible m1 muscarinic antagonist, drastically attenuated this response when given 2, but not 8, days before pilocarpine. In contrast, MT-7 did not alter the response to amphetamine (5 mg/kg). These results suggest that pilocarpine induces Fos expression in the striatum as a result of stimulating m1 muscarinic receptors located within this structure and demonstrate the utility of the MT-7 for in vivo studies of cholinergic function.
Wirtshafter
David
D
Laboratory of Integrative Neuroscience, Department of Psychology, M/C 285, University of Illinois at Chicago, 1007 W. Harrison Street, Chicago, IL 60607-7137, USA. davew@uic.edu
D000818:Animals; D002452:Cell Count / Q000379:methods; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004546:Elapid Venoms / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D018721:Muscarinic Agonists / Q000494:pharmacology*; D018727:Muscarinic Antagonists / Q000494:pharmacology; D010862:Pilocarpine / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004546:Elapid Venoms; D018721:Muscarinic Agonists; D018727:Muscarinic Antagonists; D016760:Proto-Oncogene Proteins c-fos; C419876:muscarinic toxin 7; D010862:Pilocarpine