From punch to profile.
In this essay an aspect of my research is highlighted that has its roots in the mid seventies with the implementation of the 'Palkovits punch' procedure. This methodology was introduced when radiochemical and radio-immunoassay methods became sufficiently sensitive to measure key molecules for chemical neurotransmission and metabolism in minute amounts of brain tissue. For application of today's laser guided microdissection this knowledge of functional neuro-anatomy is indispensable.
de Kloet
E Ronald
ER
Division of Medical Pharmacology, LACDR/LUMC, 9502, 2300 Leiden, RA, The Netherlands. e.kloet@lacdr.leidenuniv.nl
D001921:Brain / Q000378:metabolism; D020869:Gene Expression Profiling; D011863:Radioimmunoassay; D009435:Synaptic Transmission