[Biocompatibility of acellular urinary bladder submucosa].
OBJECTIVE
To investigate the biocompatibility of acellular urinary bladder submucosa (AUBS).


METHODS
The acellular collagen matrix of human urinary bladder submucosa was developed using freeze-thawed enzymatic treatment and freeze-drying technique. Human oral keratinocytes were cultured and seeded on AUBS at a density of 2 x 10(6)/ml in vitro. The proliferation of the cells were observed. Pockets were created in the abdominal muscle wall of 18 SD rats. AUBS in size 1 cm x 1 cm was implanted into the pocket. The grafts were observed by light microscope 3, 6, 10, 14, 21 and 28 days after operation.


RESULTS
AUBS mainly consisted of collagen fibers with a three-dimensional network structure. After the oral keratinocytes were seeded, continuous oral epithelium layer was formed on the surface of AUBS after 10 days in vitro. Histological observation of the grafted AUBS showed progressive cell infiltration at 6 days. New capillaries formed at 14 days. The collagen fibers arranged regularly at 28 days after implantation.


CONCLUSIONS
Freeze-dried AUBS may be used as a suitable scaffold for tissue regeneration, which can induce cell proliferation both in vivo and in vitro and has good biocompatibility.
Plastic Surgery Hospital, Beijing Union Medical College, Chinese Academy of Medical Sciences, Beijing 100041, P R China.
Yongqian
Y
Wang
Liqiang
L
Liu
Yangqun
Y
Li
Jiajie
J
Xu
Ran
R
Huo
Qiang
Q
Li
Senkai
S
Li
D000818:Animals; D001672:Biocompatible Materials; D049109:Cell Proliferation; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000637:transplantation*; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology; D009092:Mucous Membrane; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods; D001743:Urinary Bladder
D001672:Biocompatible Materials