MAU-8 is a Phosducin-like Protein required for G protein signaling in C. elegans.
The mau-8(qm57) mutation inhibits the function of GPB-2, a heterotrimeric G protein beta subunit, and profoundly affects behavior through the Galphaq/Galphao signaling network in C. elegans. mau-8 encodes a nematode Phosducin-like Protein (PhLP), and the qm57 mutation leads to the loss of a predicted phosphorylation site in the C-terminal domain of PhLP that binds the Gbetagamma surface implicated in membrane interactions. In developing embryos, MAU-8/PhLP localizes to the cortical region, concentrates at the centrosomes of mitotic cells and remains associated with the germline blastomere. In adult animals, MAU-8/PhLP is ubiquitously expressed in somatic tissues and germline cells. MAU-8/PhLP interacts with the PAR-5/14.3.3 protein and with the Gbeta subunit GPB-1. In mau-8 mutants, the disruption of MAU-8/PhLP stabilizes the association of GPB-1 with the microtubules of centrosomes. Our results indicate that MAU-8/PhLP modulates G protein signaling, stability and subcellular location to regulate various physiological functions, and they suggest that MAU-8 might not be limited to the Galphaq/Galphao network.
INSERM UMR 641, Université de la Méditerranée, Faculté de Médecine Secteur Nord, Boulevard Pierre Dramard, 13916 Marseille Cedex 20, France.
Caroline
C
Lacoste
Véronique
V
Barthaux
Cécile
C
Iborra
Michael
M
Seagar
Madeleine
M
Erard-Garcia
D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005136:Eye Proteins / Q000502:physiology*; D020555:GTP-Binding Protein Regulators; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000378:metabolism; D044387:GTP-Binding Protein beta Subunits / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000502:physiology*; D009154:Mutation; D010750:Phosphoproteins / Q000502:physiology*; D015398:Signal Transduction
D029742:Caenorhabditis elegans Proteins; D005136:Eye Proteins; C427883:GPB-2 protein, C elegans; D020555:GTP-Binding Protein Regulators; D044387:GTP-Binding Protein beta Subunits; D047908:Intracellular Signaling Peptides and Proteins; C511605:Mau-8 protein, C elegans; D010750:Phosphoproteins; C065631:phosducin; D019204:GTP-Binding Proteins; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D043802:GTP-Binding Protein alpha Subunits, Gq-G11