Subgenotype analysis of Cryptosporidium parvum isolates from humans and animals in Japan using the 60-kDa glycoprotein gene sequences.
Cryptosporidium parvum is a well-known intestinal parasite which is associated with severe acute diarrhea in humans and animals. This parasite is composed of morphologically identical but genetically different multiple genotypes. In humans, cryptosporidiosis is mainly caused by two C. parvum genotypes, human genotype (previously known as genotype 1 and recently proposed as new species C. hominis) and cattle genotype (previously known as genotype 2). However, recent molecular studies indicate the genetic heterogeneity among the isolates of C. parvum human or cattle genotype. Therefore, identification of the isolates at the subgenotype level is more useful for control of the Cryptosporidium infection or for understanding of the population structure of C. parvum genotypes. In the present study, we identified the subgenotypes of the C. parvum human or cattle genotype isolates from humans and animals in Japan using DNA sequencing analysis of the C. parvum 60-kDa glycoprotein gene (GP60) and showed the new subgenotype in a raccoon dog isolate. This study suggested that C. parvum cattle genotype might be composed of zoonotic and host-specific multiple subgenotypes.
Abe
Niichiro
N
Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Tennoji-ku, Osaka, 543-0026, Japan. n.abe@iphes.city.osaka.jp
Matsubayashi
Makoto
M
Kimata
Isao
I
Iseki
Motohiro
M
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D003457:Cryptosporidiosis / Q000469:parasitology* / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D017125:Genes, Protozoan; D005838:Genotype; D006801:Humans; D007564:Japan; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics*; D046031:Raccoon Dogs; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D016054:DNA, Protozoan; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; C448519:S60 glycoprotein, Cryptosporidum parvum