Relative activity of a tobacco hybrid expressing high levels of a tobacco anionic peroxidase and maize ribosome-inactivating protein against Helicoverpa zea and Lasioderma serricorne.
Tobacco (Nicotiana tabacum) plants grown from seed obtained by crossing a tobacco line that expressed an activated maize ribosome-inactivating protein (RIP) with a line that overexpressed tobacco anionic peroxidase were tested for their effects on corn earworm Helicoverpa zea and cigarette beetle Lasioderma serricorne larvae as compared to the wild-type plant cross. Significant feeding reductions were noted for transgenic plants expressing both resistance proteins as compared to wild-type plants for both H. zea and L. serricorne. Significant increases in mortality were also noted for those insects fed on the transgenic cross as compared to wild-type plants in some cases. Levels of both peroxidase and maize RIP were significantly higher in transgenic as compared to wild-type plants (which did not produce maize RIP). The degree of feeding was significantly negatively correlated with the level of RIP or peroxidase individually.
Crop BioProtection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, USA. dowdpf@ncaur.usda.gov
Patrick F
PF
Dowd
Robert A
RA
Holmes
T Scott
TS
Pinkerton
Eric T
ET
Johnson
L Mark
LM
Lagrimini
Rebecca S
RS
Boston
D000818:Animals; D001517:Coleoptera; D007915:Lepidoptera; D010544:Peroxidases / Q000235:genetics*; D010572:Pest Control, Biological; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D012270:Ribosomes; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*; D003313:Zea mays
D010940:Plant Proteins; D010544:Peroxidases; C085547:tobacco anionic peroxidase