Silencing or knocking out eukaryotic gene expression by oligodeoxynucleotide decoys.
The elucidation of molecular and signaling pathways in eukaryotic cells is often achieved by targeting regulatory element(s) found in the promoter or the enhancer region of eukaryotic gene(s) using a double-stranded (ds) oligodeoxynucleotide (ODN) containing a specific cis-element. Our laboratory is focusing on dsODN decoys containing the TGF-beta element as a novel nonsteroidal antifibrotic for achieving normal wound healing. In the model systems discussed, there is either a specific gene possessing a specific cis-element or a cluster of genes with one gene containing the consensus cis-element. The rest of the genes in the cluster contain the cis-elements homologous to this consensus element, which allows for dsODN decoy regulation of a gene cluster at one time.
Cutroneo
Kenneth R
KR
Department of Biochemistry, University of Vermont, College of Medicine, 89 Beaumont Avenue, Burlington, 05405, USA. Kenneth.cutroneo@uvm.edu
Ehrlich
H
H
D003094:Collagen / Q000378:metabolism; D005355:Fibrosis / Q000235:genetics / Q000628:therapy; D015870:Gene Expression / Q000187:drug effects*; D020868:Gene Silencing; D015316:Genetic Therapy; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage* / Q000235:genetics; D011493:Protein Kinase C / Q000378:metabolism; D020218:Response Elements / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors*; D014945:Wound Healing / Q000187:drug effects* / Q000235:genetics
D009838:Oligodeoxyribonucleotides; D016212:Transforming Growth Factor beta; D003094:Collagen; D011493:Protein Kinase C