[Preparation of soybean isoflavone aglycones by reversed-phase high performance liquid chromatography from Semen Sojae Praeparatum].
OBJECTIVE
An efficient method for the isolation of isoflavone aglycones from Semen Sojae Praeparatum was developed.


METHODS
The crude isoflavones were extracted by 75% aqueous ethanol and primarily purified by 1300 macroporous resin,and then the fractions containing daidzein, glycitein and genistein were obtained and subjected to preparative HPLC for the isolation of target components.


RESULTS
Daidzein, glycitein and genistein were obtained with purities of over 99%, etermined by analytical HPLC.


CONCLUSIONS
This technique is simple and suitable for the isolation of isoflavone aglycones from Semen Sojae Praeparatum.
Department of Pharmaceutical Analysis, College of Pharmacy, Second Military Medical University, Shanghai.
Liping
L
Qu
Heming
H
Mi
Guorong
G
Fan
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019833:Genistein / Q000032:analysis / Q000302:isolation & purification; D007529:Isoflavones / Q000032:analysis* / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*
D007529:Isoflavones; D019833:Genistein