Evaluation of steroid receptors, coregulators, and molecules associated with uterine receptivity in secretory endometria from untreated women with polycystic ovary syndrome.
OBJECTIVE
To evaluate gene and protein expression of steroid receptors, nuclear receptor coregulators, and uterine receptivity markers in midsecretory phase endometria from untreated women with polycystic ovary syndrome (PCOS).


METHODS
Case-control study.


METHODS
Hospital research unit.


METHODS
Eight patients with PCOS and eight fertile women of similar age to those with PCOS.


METHODS
Endometrial samples were obtained from women with PCOS (PCOSE) and normal (NE) women during the midsecretory phase of the menstrual cycle.


METHODS
Expression studies (immunohistochemistry, reverse transcription-polymerase chain reaction [RT-PCR] and Western blot).


RESULTS
Endometria from PCOS exhibit higher levels of messenger RNA (mRNA) and protein for estrogen receptor alpha and coactivators than NE. Epithelial cells had a greater expression of progesterone receptor in PCOSE, whereas, no differences were observed in gene and protein expression of the nuclear corepressor (NcoR) and the antiadhesion molecule mucin type-1 (MUC-1) between PCOSE and NE. Immunodetection for the coactivator ARA70 was higher in PCOSE than in NE; in contrast, expression of beta3-integrin in epithelia was lower in PCOSE than in control endometria.


CONCLUSIONS
The higher response to steroid hormones of endometria from untreated PCOS-women induces diminished expression of beta3 integrin, which partially explain implantation failure in PCOS patients.
Quezada
Susana
S
Institute of Maternal and Child Research, School of Medicine, San Borja-Arriarán Clinical Hospital, University of Chile, Santiago, Chile.
Avellaira
Carla
C
Johnson
Maria Cecilia
MC
Gabler
Fernando
F
Fuentes
Ariel
A
Vega
Margarita
M
D000328:Adult; D015153:Blotting, Western; D016022:Case-Control Studies; D010064:Embryo Implantation / Q000502:physiology*; D004717:Endometrium / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006801:Humans; D039661:Integrin beta3 / Q000096:biosynthesis / Q000235:genetics; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000503:physiopathology; D012333:RNA, Messenger / Q000096:biosynthesis; D011987:Receptors, Steroid / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D014599:Uterus / Q000737:chemistry / Q000378:metabolism
D039661:Integrin beta3; D012333:RNA, Messenger; D011987:Receptors, Steroid; D014157:Transcription Factors