Prevalence of plasmid-mediated AmpC beta-lactamases in Escherichia coli and Klebsiella pneumoniae in Korea.
Cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae are relatively prevalent in Korea, suggesting dissemination of plasmid-mediated AmpC beta-lactamases. In this study, 238 isolates of cefoxitin-resistant E. coli and K. pneumoniae (not including subspecies ozaenae and rhinoscleromatis) were collected in 2003 from 16 Korean hospitals. The prevalence of plasmid-mediated AmpC beta-lactamases was determined by PCR. The AmpC gene alleles detected in E. coli and K. pneumoniae were bla(DHA-1), 10 (8.6%) and 93 (76.2%); bla(CMY-1)-like, 14 (12.1%) and 2 (1.6%); and bla(CMY-2)-like, 38 (32.7%) and 1 (0.8%) isolates, respectively. The genes identified were bla(DHA-1), bla(CMY10)-like, and bla(CMY-2)-like, and a new variant, bla(CMY-18). Plasmidmediated AmpC gene allele-positive isolates were present both in large city and in small province hospitals, as well as in isolates from outpatients. The proportions of plasmid-mediated AmpC gene-positive isolates were similar in both expanded spectrum beta-lactamase (ESBL)-producing and -nonproducing isolates. In conclusion, DHA-1, CMY-2-like, and CMY-10-like plasmid-mediated AmpC beta-lactamase-producing K. pneumoniae and E. coli isolates are widely disseminated in both large city and small province hospitals. Absence of bla(CMY-1) and detection of a novel variant of bla(CMY-2), bla(CMY-18), indicate continued evolution of the prototype genes. Similar proportions of plasmid-mediated AmpC gene-positive isolates in both ESBL-producing and -nonproducing isolates suggest unhindered future spread of these resistances.
Lee
Kyungwon
K
Department of Laboratory Medicine, Yonsei University Hospital, 134 Shinchindong, Seodaemunku, Seoul 120-752, Korea.
Lee
Miae
M
Shin
Jong Hee
JH
Lee
Myung Hee
MH
Kang
Sung Ha
SH
Park
Ae Ja
AJ
Yong
Dongeun
D
Chong
Yunsop
Y
D001426:Bacterial Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D007723:Korea; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; C103022:beta-lactamase CMY-2; C048795:AmpC beta-lactamases; C491823:CMY-1 beta-lactamase; C491822:CMY-10 beta-lactamase; D001618:beta-Lactamases