The chinchilla microdialysis model for the study of antibiotic distribution to middle ear fluid.
In cases of slow or limited penetration of an antibiotic to the site of infection such as in acute otitis media (the middle ear), plasma levels of the agent may not reflect the concentrations that are relevant in determining clinical outcome. There is a need for a model that allows prediction of the time-course of unbound, pharmacologically active drug levels in middle ear fluid (MEF). This article introduces microdialysis as a sampling tool to measure unbound antibiotic concentrations in the MEF of the chinchilla, and briefly summarizes the results of studies of MEF penetration of a cephalosporin, a macrolide, and a ketolide antibiotic using this technique. The general concurrence of preliminary results of the chinchilla studies with clinical findings suggests that the chinchilla microdialysis model may be useful in predicting efficacy in patients.
Bioanalytic and Pharmacokinetic Services Laboratory, Department of Pharmaceutics, University of Minnesota, Minneapolis, MN, USA.
Belinda W Y
BW
Cheung
Wei
W
Liu
Ping
P
Ji
Linda L
LL
Cartier
Zhihong
Z
Li
Nael
N
Mostafa
Ronald J
RJ
Sawchuk
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use; D001826:Body Fluids / Q000187:drug effects / Q000378:metabolism; D002682:Chinchilla / Q000033:anatomy & histology / Q000378:metabolism*; D004432:Ear, Middle / Q000187:drug effects / Q000378:metabolism*; D017551:Microdialysis / Q000379:methods*; D023421:Models, Animal
D000900:Anti-Bacterial Agents