Streptococcal toxic shock syndrome caused by Streptococcus suis serotype 2.
BACKGROUND
Streptococcus suis serotype 2 (S. suis 2, SS2) is a major zoonotic pathogen that causes only sporadic cases of meningitis and sepsis in humans. Most if not all cases of Streptococcal toxic shock syndrome (STSS) that have been well-documented to date were associated with the non-SS2 group A streptococcus (GAS). However, a recent large-scale outbreak of SS2 in Sichuan Province, China, appeared to be caused by more invasive deep-tissue infection with STSS, characterized by acute high fever, vascular collapse, hypotension, shock, and multiple organ failure.


RESULTS
We investigated this outbreak of SS2 infections in both human and pigs, which took place from July to August, 2005, through clinical observation and laboratory experiments. Clinical and pathological characterization of the human patients revealed the hallmarks of typical STSS, which to date had only been associated with GAS infection. Retrospectively, we found that this outbreak was very similar to an earlier outbreak in Jiangsu Province, China, in 1998. We isolated and analyzed 37 bacterial strains from human specimens and eight from pig specimens of the recent outbreak, as well as three human isolates and two pig isolates from the 1998 outbreak we had kept in our laboratory. The bacterial isolates were examined using light microscopy observation, pig infection experiments, multiplex-PCR assay, as well as restriction fragment length polymorphisms (RFLP) and multiple sequence alignment analyses. Multiple lines of evidence confirmed that highly virulent strains of SS2 were the causative agents of both outbreaks.


CONCLUSIONS
We report, to our knowledge for the first time, two outbreaks of STSS caused by SS2, a non-GAS streptococcus. The 2005 outbreak was associated with 38 deaths out of 204 documented human cases; the 1998 outbreak with 14 deaths out of 25 reported human cases. Most of the fatal cases were characterized by STSS; some of them by meningitis or severe septicemia. The molecular mechanisms underlying these human STSS outbreaks in human beings remain unclear and an objective for further study.
Tang
Jiaqi
J
Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, China. tjq85@hotmail.com
Wang
Changjun
C
Feng
Youjun
Y
Yang
Weizhong
W
Song
Huaidong
H
Chen
Zhihai
Z
Yu
Hongjie
H
Pan
Xiuzhen
X
Zhou
Xiaojun
X
Wang
Huaru
H
Wu
Bo
B
Wang
Haili
H
Zhao
Huamei
H
Lin
Ying
Y
Yue
Jianhua
J
Wu
Zhenqiang
Z
He
Xiaowei
X
Gao
Feng
F
Khan
Abdul Hamid
AH
Wang
Jian
J
Zhao
Guo-Ping
GP
Wang
Yu
Y
Wang
Xiaoning
X
Chen
Zhu
Z
Gao
George F
GF
D000328:Adult; D000368:Aged; D000818:Animals; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D016920:Meningitis, Bacterial; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018805:Sepsis / Q000382:microbiology; D012772:Shock, Septic / Q000401:mortality* / Q000473:pathology*; D013290:Streptococcal Infections / Q000401:mortality* / Q000473:pathology*; D017011:Streptococcus suis / Q000235:genetics / Q000472:pathogenicity*; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000382:microbiology / Q000635:transmission; D014774:Virulence; D015047:Zoonoses