Sucralfate protects intestinal epithelial cells from radiation-induced apoptosis in rats.
Radiotherapy for malignant pelvic disease is often followed by acute radiation colitis (ARC). It has been reported that sucralfate treatment has a protective effect against ARC, though the mechanisms of action are unknown. The effects of sucralfate on X-ray radiation-induced apoptosis was studied at 4 Gy in the colonic crypt cells of rats. Sucralfate enemas given prior to radiation resulted in the following: (1) reduction in number of apoptotic colonic crypt cells; (2) reduction in number of caspase-3 positive cells; (3) decreases in p53 accumulation and p21 expression; (4) decreases of Bax/Bcl-2 ratio. The protective effects of sucralfate against ARC may be partially due to the suppression of radiation-induced apoptosis by way of p53 in the colon and the protection of the colonic epithelial stem cell region.
Department of Molecular Pathology, Nagasaki University Graduate School of Biomedical Sciences, Japan. f1173@cc.nagasaki-u.ac.jp
Mutsumi
M
Matsuu-Matsuyama
Kazuko
K
Shichijo
Kumio
K
Okaichi
Kouji
K
Ishii
Chung Yang
CY
Wen
Eiichiro
E
Fukuda
Toshiyuki
T
Nakayama
Masahiro
M
Nakashima
Yutaka
Y
Okumura
Ichiro
I
Sekine
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D011832:Radiation Injuries / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D013392:Sucralfate / Q000008:administration & dosage*; D016896:Treatment Outcome; D014916:Whole-Body Irradiation
D011837:Radiation-Protective Agents; D013392:Sucralfate