A new quasi-interpenetrating network formed by poly(N-acryloyl-tris-(hydroxymethyl)aminomethane and polyvinylpyrrolidone: separation matrix for double-stranded DNA and single-stranded DNA fragments by capillary electrophoresis with UV detection.
The preparation of a new separation matrix, quasi-interpenetrating networks (quasi-IPNs) formed by poly(N-acryloyl-Tris) (poly(tris-A)) and PVP, and its application for dsDNA and ssDNA fragments separation by CE with UV detection, are presented. This new quasi-IPN exhibited high sieving performance, good dynamic coating ability, and low viscosity. Single-base resolutions of dsDNA fragments (Rs = 0.92 for 123/124 bp) and ssDNA fragments (Rs = 0.65 for 123/124 base, Rs = 0.48 for 309/310 base) were achieved by using the quasi-IPN of poly(tris-A)/PVP (2% + 2%) solution in a 31 cm effective length linear polyacrylamide (LPA)-coated column. Single-base separation of dsDNA fragments (Rs = 0.92 for 123/124 bp) was also obtained within 28 min in a 46.7 cm effective length bare column at higher 160 V/cm electric field strength by using the same quasi-IPN solution. The RSD of the migration time measured for each DNA fragments was less than 1.5% in the bare column for nine continuous runs. The effects of temperature and electric field strength on the DNA separation were also investigated.
Laboratory of Analytical Chemistry, State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China.
Qian
Q
Wang
Xu
X
Xu
Lixin
L
Dai
D000179:Acrylates / Q000737:chemistry*; D000180:Acrylic Resins / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000302:isolation & purification; D004560:Electricity; D019075:Electrophoresis, Capillary / Q000379:methods*; D011205:Povidone / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D000099166:Polyacrylamides
D000179:Acrylates; D000180:Acrylic Resins; D004247:DNA; D004277:DNA, Single-Stranded; D011205:Povidone; D000099166:Polyacrylamides; C512194:poly(N-acryloyltris(hydroxymethyl)aminomethane)