Practical nerve morphometry.
Histopathological examination of peripheral nerves is often complemented by morphometric analysis in both clinical and research settings. However, existing manual or semi-automated methods are highly tedious, labour intensive and time-consuming, whereas fully automated morphometry is prone to error from the conversion of maldetected particles to spurious data. Both fully and interactive-automated morphometry have significant hardware requirements and may be difficult to implement. A new method for nerve morphometry is described aiming to combine the speed of automated morphometry with the accuracy of manual or semi-automated methods, and requiring only a digital image of the nerve section and two widely available software packages. Comparison with a standard digitizer pen method of nerve morphometry without sampling yielded statistically similar axon counts, mean area assessments and axonal area frequency distribution histograms, with assessment times of the new method between 35% and 45% of those of the standard method. This has widespread potential experimental and clinical applications and offers a means of relieving much of the tedium currently associated with nerve morphometry.
Urso-Baiarda
Fulvio
F
Restoration of Appearance and Function Trust, Mount Vernon Hospital, Middlesex HA6 2RN, UK. fulvio@doctors.org.uk
Grobbelaar
Adriaan O
AO
D000818:Animals; D001331:Automation; D001369:Axons / Q000473:pathology; D002138:Calibration; D002452:Cell Count; D005154:Facial Nerve / Q000166:cytology / Q000648:ultrastructure; D005260:Female; D007091:Image Processing, Computer-Assisted; D010525:Peripheral Nerves / Q000166:cytology* / Q000648:ultrastructure; D011817:Rabbits; D012984:Software