[Liangge san effects the expression of CD14 and scaverger receptor in the Kupffer cells of liver of endotoxemia mice].
OBJECTIVE
To study the effects of Liangge San to the expression of CD14 and scaverger receptor(SR) in the kupffer cells of liver and the pathological changes of liver tissue of endotoxemia-mice.


METHODS
The model was established with intravenous injection of lipopolysaccharide (LPS) and at the same time different dose Liangge San were given. The expression of CD14 and scaverger receptor were detected with immunohigtochemistry at the 2nd, 4th, 8th hour ofter injury and analyzed with computer image system, and the pathological changes of liver tissue were also observed.


RESULTS
At the three different hours, the expression of CD14 and scaverger receptor in macrophages of liver of LPS-injury group showed significant increase and significant decrease respectively, compared with that of the blank-control group (P < 0.01). The expression in dexamethasone group and Liangge San different dose groups were intermediate between those in injury group and those in control group. Compared with expression of LPS-injury group, those of dexamethasone group and Liangge San different dose groups showed significant differences (P < 0.01), especially that of Liangge San high dose group. Liver cells showed vacuole change. Changes of CD14 and SR expression were paralleled with the severity of liver damages of the mice.


CONCLUSIONS
Liangge San can inhibite the up-regulation of CD14 expression and down-regulation of scaverger receptor expression in a dosage-dependent manner and also alleviate the damages of liver induced by LPS.
Nanfang Medical University, Guangzhou 510515, China. yulzh@fim-mu.com
Lin-Zhong
LZ
Yu
Ai-Da
AD
Jiang
Yu-Yao
YY
Cheng
Hui
H
Lin
Qing-He
QH
Qin
Xiao-Dong
XD
Ma
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D019446:Endotoxemia / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D005260:Female; D007728:Kupffer Cells / Q000378:metabolism / Q000473:pathology; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008070:Lipopolysaccharides; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D051116:Receptors, Scavenger / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D051116:Receptors, Scavenger; D003907:Dexamethasone