The uptake and fate of exogenous cellular DNA in mammalian cells.
Mammalian cells take up exogenous DNA very inefficiently. However, in the absence of viral vectors, DNA can be transfected into cells by co-precipitation with calcium phosphate and usually also with carrier DNA or by lipofection or electroporation. Such DNA can be expressed efficiently by cells. Alternatively, direct injection can also result in uptake and expression of transgenes. Without carefully designed means to target DNA specifically to integrate into the host genome, the vast majority of internalised DNA remains extra-chromosomal and is degraded. The likely fate of DNA which in low levels may contaminate vaccines derived from mammalian cell lines, will also be destruction. There is a theoretical risk of DNA integration events with random sequences of donor-derived DNA but the probability of that leading to serious adverse effects to the host is extremely small.
Liver and Hepatobiliary Unit Queen Elizabeth Hospital, School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK. a.j.strain@bham.ac.uk
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Strain
D000284:Administration, Oral; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D004705:Endocytosis; D011992:Endosomes / Q000502:physiology; D006801:Humans; D008322:Mammals; D019444:Vaccines, DNA / Q000008:administration & dosage
D019444:Vaccines, DNA; D004247:DNA; D003851:Deoxyribonucleases