Transient incorporation of native GluR2-lacking AMPA receptors during hippocampal long-term potentiation.
Postnatal glutamatergic principal neuron synapses are typically presumed to express only calcium-impermeable (CI), GluR2-containing AMPARs under physiological conditions. Here, however, we demonstrate that long-term potentiation (LTP) in CA1 hippocampal pyramidal neurons causes rapid incorporation of GluR2-lacking calcium-permeable (CP)-AMPARs: CP-AMPARs are present transiently, being replaced by GluR2-containing AMPARs approximately 25 min after LTP induction. Thus, CP-AMPARs are physiologically expressed at CA1 pyramidal cell synapses during LTP, and may be required for LTP consolidation.
MRC Centre for Synaptic Plasticity, Department of Anatomy, University of Bristol, School of Medical Sciences, Bristol BS8 1TD, UK.
Karen
K
Plant
Kenneth A
KA
Pelkey
Zuner A
ZA
Bortolotto
Daiju
D
Morita
Akira
A
Terashima
Chris J
CJ
McBain
Graham L
GL
Collingridge
John T R
JT
Isaac
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D006624:Hippocampus / Q000166:cytology*; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D051379:Mice; D018733:Nicotinic Antagonists / Q000494:pharmacology; D018408:Patch-Clamp Techniques / Q000379:methods; D011073:Polyamines / Q000494:pharmacology; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D051381:Rats; D018091:Receptors, AMPA / Q000737:chemistry / Q000172:deficiency / Q000502:physiology*; D013997:Time Factors
D018733:Nicotinic Antagonists; D011073:Polyamines; D018091:Receptors, AMPA; C034092:delta-philanthotoxin; C104722:glutamate receptor ionotropic, AMPA 2; D002118:Calcium