[Studies on chemical constituents of the brown alga Dictyopteris divaricata].
OBJECTIVE
To investigate the chemical constituents of the brown alga D. divaricata, and to test cytotoxicities of the purified compounds.


METHODS
Compounds were isolated by normal phase silica gel, Sephadex LH-20 chromatography and reverse phase HPLC techniques. Their structures were elucidated by spectroscopic methods including IR, MS and NMR. Cytotoxicities were tested by MTT method.


RESULTS
Eight compounds were isolated from ethanolic extract of the brown alga D. divaricata and their structures were identified as (-)-torreyol (I), 4beta, 5alpha-dihydroxycubenol (II), 3-farnesyl-p-hydroxybenzioc acid (III), chromazonarol (IV), fucosterol (V), phenyl acetylamine (VI), 4-hydroxybenzoic acid (VII) and n-hexadecanoic acid (VIII).


CONCLUSIONS
Compound II and IV were obtained from this alga for the first time. The others were isolated from the Dictyotaceae algae for the first time. All compounds were inactive (IC50 > 10 microg x mL(-1)) against human tumor cell lines KB, Bel-7402, PC-3M, Ketr 3 and MCF-7.
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Fu-Hang
FH
Song
Xiao
X
Fan
Xiu-Li
XL
Xu
Su-Juan
SJ
Wang
Shuai
S
Li
Yong-Chun
YC
Yang
Jian-Gong
JG
Shi
D045744:Cell Line, Tumor / Q000187:drug effects; D006801:Humans; D010226:Parabens / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000459:Phaeophyceae / Q000737:chemistry*; D013265:Stigmasterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014966:Xanthenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
C511974:4beta,5alpha-dihydroxycubenol; D010226:Parabens; D013729:Terpenes; D014966:Xanthenes; C015896:fucosterol; C121655:chromazonarol; D013265:Stigmasterol; C038193:4-hydroxybenzoic acid