GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells.
RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lalpha, IL-lbeta, IL-6, IL-10, H-2Kb, I-Ab, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFbeta, TNFalpha and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells.
Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Room CA4124, 1120 15th Street, Augusta, GA 30912, United States.
Degui
D
Geng
Sunil K
SK
Joshi
Robert
R
Podolsky
Jin-Xiong
JX
She
D000818:Animals; D017951:Antigen Presentation; D015703:Antigens, CD / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D007155:Immunologic Factors / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000276:immunology; D034622:RNA Interference; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction / Q000276:immunology*
D015703:Antigens, CD; D016207:Cytokines; D007155:Immunologic Factors; D016188:Receptors, Granulocyte Colony-Stimulating Factor