Broth conditions determining specific cake resistance during microfiltration of Bacillus subtilis.
The effects of broth pH, pressure, temperature, and fermentation medium on specific cake resistance were studied for dead-end microfiltration of Bacillus subtilis. Decreases in pH and transmembrane pressure decreased the specific cake resistance for cells grown in both complex and defined media. With the complex medium, the reduction in resistance with temperature decrease did not offset the flux decrease caused by the increase in viscosity. The greatest decrease in specific cake resistance occurred with adjustment of pH to 7.5 for cells grown in defined medium. For those cells the change in pH resulted in aggregation leading to a large increase in flux.
Department of Chemical Engineering, Iowa State University, Ames, Iowa 50011, USA.
Kevin
K
Graves
Glenn
G
Rozeboom
Meng
M
Heng
Charles
C
Glatz
D001412:Bacillus subtilis / Q000201:enzymology / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D015202:Protein Engineering; D020860:Subtilisin / Q000378:metabolism; D013696:Temperature; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods; D014783:Viscosity
D003470:Culture Media; D008567:Membranes, Artificial; D020860:Subtilisin