Simple and rapid liquid chromatography method for determination of efavirenz in plasma.
A simple and rapid high performance liquid chromatographic method for determination of efavirenz in human plasma was developed. The method involved extraction of sample with ethyl acetate and analysis using a reversed-phase C(18) column (150 mm) with UV detection. The assay was linear from 0.0625 to 10.0 microg/ml. The method was specific for efavirenz estimation and the drug was stable in plasma up to one month at -20 degrees C. The average recovery of efavirenz from plasma was 101%. Due to its simplicity, the assay can be used for pharmacokinetic studies and therapeutic drug monitoring of efavirenz.
Ramachandran
Geetha
G
Tuberculosis Research Centre, Indian Council of Medical Research, Chetput, Chennai, India.
Kumar
A K Hemanth
AK
Swaminathan
Soumya
S
Venkatesan
P
P
Kumaraswami
V
V
Greenblatt
David J
DJ
D000480:Alkynes; D019380:Anti-HIV Agents / Q000097:blood; D048588:Benzoxazines; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003521:Cyclopropanes; D004355:Drug Stability; D006801:Humans; D010078:Oxazines / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000480:Alkynes; D019380:Anti-HIV Agents; D048588:Benzoxazines; D003521:Cyclopropanes; D010078:Oxazines; C098320:efavirenz