GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells.
RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lalpha, IL-lbeta, IL-6, IL-10, H-2Kb, I-Ab, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFbeta, TNFalpha and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells.
Geng
Degui
D
Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Room CA4124, 1120 15th Street, Augusta, GA 30912, United States.
Joshi
Sunil K
SK
Podolsky
Robert
R
She
Jin-Xiong
JX
D000818:Animals; D017951:Antigen Presentation; D015703:Antigens, CD / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D007155:Immunologic Factors / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000276:immunology; D034622:RNA Interference; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction / Q000276:immunology*
D015703:Antigens, CD; D016207:Cytokines; D007155:Immunologic Factors; D016188:Receptors, Granulocyte Colony-Stimulating Factor