Identification of steviol glucuronide in human urine.
Stevioside (250 mg capsules) was given three times daily to 10 healthy subjects. Steviol glucuronide (steviol 19-O-beta-D-glucopyranosiduronic acid; MM, 494.58; melting point, 198-199 degrees C) was characterized in the 24 h urine as the only excretion product of oral stevioside by MS, NMR, IR, and UV spectroscopy. This is the first report on the unambiguous identification of steviol glucuronide in human urine.
Laboratory of Functional Biology, KULeuven, Kasteelpark Arenberg 31, B-3001 Leuven, Belgium. Jan.Geuns@bio.kuleuven.be
Jan M C
JM
Geuns
Johan
J
Buyse
Annelies
A
Vankeirsbilck
Elisabeth H M
EH
Temme
Frans
F
Compernolle
Suzanne
S
Toppet
D045786:Diterpenes, Kaurane / Q000008:administration & dosage / Q000652:urine*; D005960:Glucosides / Q000008:administration & dosage; D020719:Glucuronides / Q000652:urine*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D045786:Diterpenes, Kaurane; D005960:Glucosides; D020719:Glucuronides; C012043:stevioside; C012042:steviol