Lentiviral vectors.
We review the use of lentiviral vectors in current human gene therapy applications that involve genetic modification of nondividing tissues with integrated transgenes. Safety issues, including insertional mutagenesis and replication-competent retroviruses, are discussed. Innate cellular defenses against retroviruses and their implications for human gene therapy with different lentiviral vectors are also addressed.
Loewen
Nils
N
Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA. N-loewen@northwestern.edu
Poeschla
Eric M
EM
D015316:Genetic Therapy; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D016086:Lentivirus / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication