Cerebral metabolites in HIV-infected children followed for 10 months with 1H-MRS.
BACKGROUND
Previous studies have shown that HIV-infected children have abnormal cerebral metabolites, measured by proton MR spectroscopy (1H MRS), but the stability of these measurements over time has not been described in HIV-infected children. The authors recently reported a study of cerebral metabolites in 20 HIV-infected children (6 to 16 years of age); the current study followed 12 of these children (10.0 years +/- 3.7 years) and repeated the MR spectroscopy at 24.1 +/- 3.7 weeks and 42.2 +/- 3.5 weeks following the entry time with repeated neuropsychological testing.


METHODS
1H MR spectra were acquired at 1.5 T (GE Signa, PRESS localization, repetition time = 3,000 msec, echo time = 30 msec). Five brain regions were studied: right frontal white matter, left frontal white matter, right basal ganglia, right hippocampus, and midfrontal gray matter. The concentrations of N-acetylaspartate (NAA), choline (CHO), creatine (CR), and myo-inositol (mI) and the ratio of each metabolite to CR were determined.


RESULTS
There were no changes in the metabolite concentrations or metabolite/CR ratios at the three time periods. Similarly, during this follow-up period, HIV-positive children showed no changes in clinical signs, HIV viral loads, CD4%, or CD4 counts, except for improved spatial memory with repeat testing.


CONCLUSIONS
In a clinically and neurologically stable group of HIV-infected children, cerebral metabolites were stable over a 10-month time period, suggesting that it is possible to assess changes in cerebral metabolites as a measure of cerebral health, but longer follow-up in a larger sample is needed.
Los Angeles Biomedical Research Institute, Harbor-UCLA Medical Center, Torrance, CA 90502, USA. keller@labiomed.org
M A
MA
Keller
T N
TN
Venkatraman
M A
MA
Thomas
A
A
Deveikis
C
C
Lopresti
J
J
Hayes
N
N
Berman
I
I
Walot
T
T
Ernst
L
L
Chang
D000293:Adolescent; D001921:Brain / Q000378:metabolism*; D002648:Child; D005500:Follow-Up Studies; D015658:HIV Infections / Q000378:metabolism*; D015497:HIV-1; D006801:Humans; D008137:Longitudinal Studies; D009682:Magnetic Resonance Spectroscopy / Q000706:statistics & numerical data; D011522:Protons
D011522:Protons