Characterization of quantum dot bioconjugates by capillary electrophoresis with laser-induced fluorescent detection.
In this paper, we present a universal, highly efficient and sensitive method for the characterization of quantum dot (QD) bioconjugates based on capillary electrophoresis with laser-induced fluorescent (LIF) detection. We first prepared CdTe QDs in aqueous phase by a chemical route with mercaptopropionic acid as a ligand, and then were coupled to certain proteins using bifunctional linkage reagent or electrostatic attraction. The QD bioconjugates were characterized by capillary electrophoresis with LIF detection. We found that QD bioconjugates were efficiently separated with free QDs by the optimization of buffer pH. Furthermore, we found that ultrafiltration was an effective and simple approach to purify QD conjugates with bovine serum albumin (BSA). Due to their broad absorption spectra and size dependent emission wavelength tunability, QDs can be excited to emit different colour fluorescence using a single wavelength laser source, and therefore, we believe that CE with LIF detection will become a universal and efficient tool for the characterization of QD bioconjugates.
Huang
Xiangyi
X
College of Chemistry & Chemical Engineering, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China.
Weng
Jifang
J
Sang
Fuming
F
Song
Xingtao
X
Cao
Chengxi
C
Ren
Jicun
J
D019075:Electrophoresis, Capillary / Q000379:methods*; D007834:Lasers; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence / Q000379:methods*