Soluble sustained release gene delivery system.
Delivery of genetic substance to target cells remains an obstacle for efficient utilization of gene therapy approaches. In this study, we describe a formulation of methacrylate acid copolymer carrier of DNA, in which the release rate of the gene can be controlled by pH. Plasmid release was coupled with the polymer's dissolution, which was accelerated in alkali conditions. The released plasmid was intact and bioactive, although alteration from closed circular supercoil to relaxed conformation was observed. Confocal laser scanning microscopy detected the plasmid DNA along the central layers of the polymeric film. Gene delivery systems controlled by the dissolution of the polymeric films offer flexibility in quantity and size of the incorporated DNA, and therefore could have a potential for in vivo use.
Bachrach
Gilad
G
Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, PO Box 12272, Jerusalem 91120, Israel.
Friedman
Michael
M
Gilinski
Gladis
G
Steinberg
Doron
D
D001672:Biocompatible Materials; D004247:DNA / Q000008:administration & dosage*; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D015316:Genetic Therapy / Q000379:methods*; D010957:Plasmids; D011109:Polymethacrylic Acids
D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D011109:Polymethacrylic Acids; D004247:DNA