Post-transcriptional downregulation of sarcolipin mRNA by triiodothyronine in the atrial myocardium.
Thyroid hormone-mediated positive cardiotropic effects are differently regulated between the atria and ventricles. This regulation is, at least in part, dependent on sarcoplasmic reticulum (SR) proteins. Sarcolipin, a homologue of phospholamban, has been recently identified as an atrium-specific SR protein. The expression of sarcolipin mRNA was significantly decreased in the atria of mice with hyperthyroidism and in 3,5,3'-triiodo-l-thyronine-treated neonatal rat atrial myocytes. Promoter activity and mRNA stability analyses revealed that thyroid hormone post-transcriptionally down regulated the expression of sarcolipin mRNA. The atrium-specific effect of thyroid hormone may occur in part through the regulation of atrial sarcolipin gene expression.
Minamisawa
Susumu
S
Department of Physiology, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan. sminamis@med.yokohama-cu.ac.jp
Uemura
Nobuyuki
N
Sato
Yoji
Y
Yokoyama
Utako
U
Yamaguchi
Teruhide
T
Inoue
Kazuhide
K
Nakagome
Masamichi
M
Bai
Yunzhe
Y
Hori
Hideaki
H
Shimizu
Mitsuyuki
M
Mochizuki
Seibu
S
Ishikawa
Yoshihiro
Y
D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects*; D006325:Heart Atria / Q000378:metabolism; D006980:Hyperthyroidism / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000235:genetics; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011510:Proteolipids / Q000096:biosynthesis* / Q000235:genetics; D020871:RNA Stability / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000187:drug effects; D014284:Triiodothyronine / Q000008:administration & dosage*; D000099211:Phospholamban
D002135:Calcium-Binding Proteins; D009124:Muscle Proteins; D011510:Proteolipids; D012333:RNA, Messenger; D014284:Triiodothyronine; D000099211:Phospholamban; C077258:sarcolipin