[Growth and differentiation of adult canine autologous skeletal myoblasts after transplanted into acute myocardial infarction region].
OBJECTIVE
To study the growth and differentiation of adult canine autologous skeletal myoblasts after being transplanted into acute myocardial infarction (AMI) region by intramyocardium injection (IMI) and intracoronary infusion (ICI).


METHODS
Autologous skeletal myoblasts were procured by a modified method. AMI model of adult canine was obtained through left anterior descending branch ligation and was divided into 4 groups (n = 5 for each group). Autologous skeletal myoblasts (1.0 - 1.4 x 10(8) cells) were injected locally into AMI region or infused into infarction-related coronary artery. Specimens were harvested 4 weeks after cellular transplantation for histological study including HE, PTH, immunochemical stain and transmission electronmicroscope.


RESULTS
In both two transplantation groups, newborn muscle-derived cells, striated muscle tissue and mature skeletal myofibril were demonstrated existing in MI region by electronmicroscope, PTH stain or anti-skeletal myosin heavy chain (slow) immunochemical stain, respectively. Newborn striated muscle tissues arranged in order of consistency with host myocardial fibers in two treatment groups. Newborn striated muscle tissue was more inclined to gather in MI region in the local injection group but distracted from each other in the intracoronary infusion group.


CONCLUSIONS
Autologous skeletal myoblasts appears to live and differentiate into mature striated muscle tissue after transplanting into AMI region by IMI or ICI routes.
Department of Coronary Heart Disease, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.
Ke-fei
KF
Dou
Yue-jin
YJ
Yang
Wei-xian
WX
Yang
Hong
H
Qiu
Yi
Y
Tian
Ying-mao
YM
Ruan
Ji-lin
JL
Chen
Run-lin
RL
Gao
Zai-jia
ZJ
Chen
D000818:Animals; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D008297:Male; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000637:transplantation*; D009203:Myocardial Infarction / Q000601:surgery*; D014182:Transplantation, Autologous