Relative activity of a tobacco hybrid expressing high levels of a tobacco anionic peroxidase and maize ribosome-inactivating protein against Helicoverpa zea and Lasioderma serricorne.
Tobacco (Nicotiana tabacum) plants grown from seed obtained by crossing a tobacco line that expressed an activated maize ribosome-inactivating protein (RIP) with a line that overexpressed tobacco anionic peroxidase were tested for their effects on corn earworm Helicoverpa zea and cigarette beetle Lasioderma serricorne larvae as compared to the wild-type plant cross. Significant feeding reductions were noted for transgenic plants expressing both resistance proteins as compared to wild-type plants for both H. zea and L. serricorne. Significant increases in mortality were also noted for those insects fed on the transgenic cross as compared to wild-type plants in some cases. Levels of both peroxidase and maize RIP were significantly higher in transgenic as compared to wild-type plants (which did not produce maize RIP). The degree of feeding was significantly negatively correlated with the level of RIP or peroxidase individually.
Dowd
Patrick F
PF
Crop BioProtection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, USA. dowdpf@ncaur.usda.gov
Holmes
Robert A
RA
Pinkerton
T Scott
TS
Johnson
Eric T
ET
Lagrimini
L Mark
LM
Boston
Rebecca S
RS
D000818:Animals; D001517:Coleoptera; D007915:Lepidoptera; D010544:Peroxidases / Q000235:genetics*; D010572:Pest Control, Biological; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D012270:Ribosomes; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*; D003313:Zea mays
D010940:Plant Proteins; D010544:Peroxidases; C085547:tobacco anionic peroxidase