Silver staining method for DNA in polyacrylamide gels using eriochrome black T as a silver-ion sensitizer.
A sensitive silver staining method using eriochrome black T as a silver-ion sensitizer for DNA detection in polyacrylamide gels was developed. The sensitivity of this staining method was significantly improved by the new silver-ion sensitizer containing a diazo group, which has reducing power. The staining method lasted a total of approximately 15 min following a fixing step for 2 x 20 min. The detection limit of this staining method was 1-4 pg for PhiX174 DNA/HaeIII in both nondenaturing and denaturing polyacrylamide gels. This staining method was especially effective in low-base pair DNA, with a sensitivity that was approximately ten-fold higher than previously published silver staining methods.
College of Pharmacy, Chonnam National University, Buk-ku, Kwangju, South Korea.
Sun-young
SY
Hwang
Li-tai
LT
Jin
Gyurng-soo
GS
Yoo
Jung-Kap
JK
Choi
D000180:Acrylic Resins / Q000737:chemistry*; D001391:Azo Compounds / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D012834:Silver / Q000737:chemistry; D016622:Silver Staining / Q000379:methods*
D000180:Acrylic Resins; D001391:Azo Compounds; C016680:polyacrylamide gels; D012834:Silver; D004247:DNA; C014527:Eriochrome Black T