Characterization of diffusion of macromolecules in konjac glucomannan solutions and gels by fluorescence recovery after photobleaching technique.
Konjac glucomannan (KGM) is a neutral polysaccharide with interesting properties as gelling agent and thickener. Its peculiar biodegradability, being not degradable in the small intestine but degradable by the anaerobic human intestinal bacteria, turn it into a promising candidate for colonic drug delivery systems. In this study aqueous systems (0.5%, w/v,) of KGM from three different origins and their mixtures with xanthan gum (XG) (1:1) were evaluated as regards their rheological properties and the diffusion coefficients and mobile fraction of macromolecules (dextrans of different molecular weight). Rheological data illustrate the synergism between KGM and XG at a stoichiometric relationship 1:1. Moreover, fluorescence recovery after photobleaching (FRAP) data indicate that diffusion of probes through the polysaccharide systems cannot be completely explained by the macroscopic properties of the medium but it is related to their molecular size and as a consequence to a sieving mechanism. The strong differences between KGM from different suppliers suggest the convenience of establishing specifications for this material in order to use it as pharmaceutical excipient.
Alvarez-Manceñido
Felipe
F
Departamento Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, Santiago de Compostela, Spain.
Braeckmans
Kevin
K
De Smedt
Stefaan C
SC
Demeester
Josepth
J
Landin
Mariana
M
Martínez-Pacheco
Ramón
R
D003911:Dextrans / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D036681:Fluorescence Recovery After Photobleaching; D005782:Gels; D008351:Mannans / Q000737:chemistry*; D018613:Microscopy, Confocal; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D012212:Rheology; D012995:Solubility; D012996:Solutions
D003911:Dextrans; D004337:Drug Carriers; D005782:Gels; D008351:Mannans; D011135:Polysaccharides, Bacterial; D012996:Solutions; C015219:fluorescein isothiocyanate dextran; C022901:(1-6)-alpha-glucomannan; D016650:Fluorescein-5-isothiocyanate; C002563:xanthan gum