Expanded bed adsorption/desorption of proteins with Streamline Direct CST I adsorbent.
Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out for two-component proteins (BSA and myoglobin) competitive adsorption and desorption in an expanded bed packed with Streamline Direct CST I. Based on the measurements of the single- and two-component bovine serum albumin (BSA)/myoglobin adsorption isotherm on Streamline Direct CST I, the binding and elution conditions for the whole EBA process are selected; and then frontal analysis for a longer timescale and column displacement experiments in a fixed bed (XK16/20 column) are carried out to evaluate the two-component proteins (BSA and myoglobin) competitive adsorption and displacement on Streamline Direct CST I. Finally, the feasibility of capturing both BSA and myoglobin by an expanded bed packed with Streamline Direct CST I is addressed in a Streamline 50 column packed with 300 mL Streamline Direct CST I.
Li
Ping
P
Department of Chemical Engineering, Laboratory of Separation and Reaction Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n 4200-465 Porto, Portugal.
Xiu
Guohua
G
Mata
Vera G
VG
Grande
Carlos A
CA
Rodrigues
Alirio E
AE
D000327:Adsorption; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D009211:Myoglobin / Q000302:isolation & purification*; D010865:Pilot Projects; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000295:instrumentation* / Q000379:methods
D009211:Myoglobin; D012710:Serum Albumin, Bovine