[A micro-culture method for continuous observation of free-living amoebae].
OBJECTIVE
To establish a method for co-culture of amoebae and endosymbionts, also for continuously observing the microphenotype of amoebae.


METHODS
24 wells culture plate with cover glass on the wells was used as containers. Amoebae and Candida albicans were co-cultured in microdrop of medium in the wells at 37 degrees C, and observed under x1000.


RESULTS
Continuous observation revealed trophozoites in various shapes like letters T, K, or Y, their movement and ingestion phenomenon were observed.


CONCLUSIONS
The micro-culture method is useful in observing the amoebal morphology and its phagocytic process to Candida albicans.
Chen
Hong-you
HY
Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China.
Jiang
Qing-wu
QW
Li
Qin-xue
QX
Li
Zi-hua
ZH
D000656:Amoeba / Q000254:growth & development* / Q000502:physiology; D000818:Animals; D002176:Candida albicans; D018920:Coculture Techniques; D003470:Culture Media; D010587:Phagocytosis; D013696:Temperature
D003470:Culture Media