[Determination of aristolochic acid A in Guanxinsuhe preparations by RP-HPLC].
OBJECTIVE
To establish a determination method of aristolochic acid A in Guanxisuhe preparations by RP-HPLC.


METHODS
The instrument used was Hewlett-Packard 1100 HPLC with a Alltech C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase was methanol-water-acetic acid (68: 32:1) and the flow rate was 1.0 mL x min(-1). The UV detection wavelength was 390 nm and the column temperature was at 35 degrees C. The extracted solvent for the preparations was methanol solution contained 10% formic acid.


RESULTS
The calibration curve was linear (r = 0.999 9) within the range of 0.119-1.89 microg for aristolochic acid A. The average recovery 99.0%, RSD 0.63%.


CONCLUSIONS
The method with good linear relationship was convenient, quick, accurate, and suitable for the quality control of the aristolochic acid A in Guanxinsuhe and other traditional Chinese medicines containing aristolochic acid A.
Institute of Chinese Materia Medica, China Academy of Chinese Medicao Sciences, Beijing 100700, China.
Lin
L
Li
Hui-Min
HM
Gao
Zhi-Min
ZM
Wang
Wei-Hao
WH
Wang
D030022:Aristolochia / Q000737:chemistry*; D034341:Aristolochic Acids / Q000032:analysis*; D002214:Capsules; D002316:Cardiotonic Agents / Q000008:administration & dosage / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D031317:Liquidambar / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control
D034341:Aristolochic Acids; D002214:Capsules; D002316:Cardiotonic Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C000228:aristolochic acid I