Monkey embryonic stem cells differentiate into adipocytes in vitro.
Production of functional adipocytes is important in adipocyte research and regenerative medicine. In this paper, we describe the differentiation of monkey embryonic stem (ES) cells into insulin-responsive adipocytes. Treatment of embryoid body (EB) outgrowth with adipogenic hormones induced the expression of adipocyte-specific genes, such as PPARgamma, C/EBPalpha, aP2, insulin receptor, and GLUT4. Expression of adipocytokines, leptin and adiponectin, was also detected. Furthermore, translocation of GLUT4 was observed by insulin stimulation in differentiated adipocytes. These results suggested that monkey ES cells can be a useful tool for studying adipogenesis in primate.
Yamashita
Akihiro
A
Research Center for Animal Life Science, Shiga University of Medical Science, Seta, Ohtsu, Japan.
Takada
Tatsuyuki
T
Omatsu-Kanbe
Mariko
M
Nemoto
Ken-Ichi
K
Matsuura
Hiroshi
H
Yamamoto
Gaku
G
Torii
Ryuzo
R
D017667:Adipocytes / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D050156:Adipogenesis; D052242:Adiponectin / Q000032:analysis / Q000235:genetics; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000032:analysis / Q000235:genetics; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D051275:Glucose Transporter Type 4 / Q000032:analysis / Q000235:genetics; D007150:Immunohistochemistry; D007328:Insulin / Q000494:pharmacology; D020738:Leptin / Q000032:analysis / Q000235:genetics; D008252:Macaca fascicularis; D047495:PPAR gamma / Q000032:analysis / Q000235:genetics; D011972:Receptor, Insulin / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D014178:Translocation, Genetic / Q000187:drug effects
D052242:Adiponectin; D022763:CCAAT-Enhancer-Binding Protein-alpha; D016207:Cytokines; D051275:Glucose Transporter Type 4; D007328:Insulin; D020738:Leptin; D047495:PPAR gamma; D011972:Receptor, Insulin