Quick and efficient purification of Giardia intestinalis cysts from fecal samples.
Giardia intestinalis is an intestinal parasite that has sparked considerable interest because of the public health problem it creates and because it is regarded as one of the earliest divergent eukaryotes. The present report describes a new method for quick, clean, and effective isolation of G. intestinalis cysts from fecal samples. The isolated cysts have the quality required for biochemical studies of the excystation process.
Laboratorio de Investigaciones Básicas en Bioquímica LIBBIQ, Departamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, Colombia. mwassermannl@unal.edu.co
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Alvarado
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Wasserman
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000469:parasitology*; D016829:Giardia lamblia / Q000737:chemistry / Q000166:cytology / Q000302:isolation & purification*; D008853:Microscopy; D010274:Parasitology / Q000379:methods*; D015800:Protozoan Proteins / Q000032:analysis
D015800:Protozoan Proteins