Morphological and binding properties of interleukin-6 on thin ZnO films grown on (100) silicon substrates for biosensor applications.
To develop effective protein immobilization technology with minimal amounts of protein for high sensitivity surface acoustic wave biosensors, we determined the binding properties, and morphological characteristics of human interleukin-6 (IL-6), a pro-inflammatory cytokine, on the surface of ZnO, and SiO(2) films grown onto (100) Si substrates, for the first time. Interleukin-6 was immobilized in the range of 0.276-10 pg/ml on the surface of ZnO and SiO(2), and visualized at each stage, while protein-protein interactions were measured with the antigen/antibody immunoassay of solid-phase ELISA, which we modified for these types of substrates. A relative mass value was determined in each case. ELISA detected upward of 1 and 6 ng/ml of protein applied on ZnO and SiO(2), respectively. It is concluded that the more reactive ZnO surface is a new and more effective template for protein immobilization.
Krishnamoorthy
Soumya
S
Department of Electrical and Computer Engineering, University of Maryland, College Park, MD 20742, USA.
Bei
Thaleia
T
Zoumakis
Emmanouil
E
Chrousos
George P
GP
Iliadis
Agis A
AA
D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003460:Crystallization / Q000379:methods; D007118:Immunoassay / Q000379:methods*; D015850:Interleukin-6 / Q000737:chemistry*; D008422:Materials Testing; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000648:ultrastructure; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties; D015034:Zinc Oxide / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D015850:Interleukin-6; D012710:Serum Albumin, Bovine; D015034:Zinc Oxide; D012825:Silicon