[Isolation of BADH gene promoter from Suaeda liaotungensis and its sequence analysis].
In this study, the 5' -flanking proximal region of stress-induced gene encoding betaine aldehyde dehydrogenase was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis. 1993 bp sequence was obtained by sequencing. The transcription start site, which localized at 62 bases upstream of the start ATG, was predicted using TSSP-TCM program. The functional elements were analysed by PLACE programm. The SlBADH gene promoter contains the basic elements: TATA-box, CAAT-box, and stress-induced elements: salt responsed element, cold, dehydration, ABA and frozen responsed elements, WUN responsed elements and HSE. Obtaining the promoter of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis provides a foundation for analyzing the stress-induced promoter elements, studying the relationship between structure and founction of the promoter, and investigating the molecular mechanism of BADH gene regulation.
Li
Qiu-Li
QL
College of Life Science, Liaoning Normal University, Dalian 116029, China. liqiuli@dl.cn
Zhang
Yi
Y
Yin
Hui
H
Li
Dan
D
D001483:Base Sequence; D050728:Betaine-Aldehyde Dehydrogenase / Q000235:genetics*; D019663:Chenopodiaceae / Q000201:enzymology* / Q000235:genetics; D003080:Cold Temperature; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D012492:Salts; D017422:Sequence Analysis, DNA; D024363:Transcription Initiation Site
D010940:Plant Proteins; D012492:Salts; D050728:Betaine-Aldehyde Dehydrogenase