[Study on the preparation of resveratrol chitosan nanoparticles with free amino groups on the surface].
OBJECTIVE
To prepare resveratrol chitosan nanoparticles with free amine groups on the surface so as to conjugate ligands, which will actively target to special tissues or organs.


METHODS
The chitosan nanoparticles with free amine on the surface was prepared by sodium chloride precipitation. Nanoparticles with different solidification degrees were studied on turbidity, in vitro release, encapsulation efficiency, drug loading and diameter.


RESULTS
The turbidity of nanoparticles with various solidification degrees decreased at different rates after ultrasonic or water bath heating treatment. All nanoparticles mentioned above obviously shew sustained release. The rate of release was slowed down with the increase of solidification agents. Solidification had no obvious effects on the encapsulation efficiency and drug loading. The diameter of chitosan nanoparticles with 200 microL solidification agents was 487 nm. The polydispersion was 0.144.


CONCLUSIONS
The diameter of the prepared nanoparticles was relatively small. The amine on the surface was free, which offered the possibility of designing the acive target drug delivery system.
Yao
Qian
Q
West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Hou
Shi-Xiang
SX
He
Wei-Ling
WL
Feng
Ji-Liang
JL
Wang
Xin-Chuni
XC
Fei
Hong-Xin
HX
Chen
Zheng-Hua
ZH
D048271:Chitosan / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods*; D016503:Drug Delivery Systems; D049329:Nanostructures; D010316:Particle Size; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage* / Q000737:chemistry
D013267:Stilbenes; D048271:Chitosan; D000077185:Resveratrol