Rapid determination of fumonisin B1 in food samples by enzyme-linked immunosorbent assay and colloidal gold immunoassay.
A rapid enzyme-linked immunosorbent assay (ELISA) test (microwell plate) and a membrane-based colloidal gold immunoassay in flow-through and lateral-flow formats for the rapid detection of fumonisin B1 (FB1) were developed. The rapid microwell assay can be completed within 20 min with the detection limit of 0.5 +/- 0.2 microg/L. Membrane-based colloidal gold immunoassays had a visual detection limit of 1.0 microg/L for FB1 with the detection time of <10 min. Matrix interference was eliminated by 15-fold dilutions of methanol extracts with buffer. These immunoassays can be used as quantitative or qualitative tools for the rapid detection of FB1 residues in 10-20 min on-site.
Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnolgy, Tianjin University of Science and Technology, Tianjin 300222, People's Republic of China. elisasw2002@yahoo.com.cn
Shuo
S
Wang
Ying
Y
Quan
Nanju
N
Lee
Ivan R
IR
Kennedy
D010367:Arachis / Q000737:chemistry; D002523:Edible Grain / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D037341:Fumonisins / Q000032:analysis*; D017979:Gold Colloid; D007118:Immunoassay; D045868:Sorghum / Q000737:chemistry
D037341:Fumonisins; D017979:Gold Colloid; C056933:fumonisin B1