SPR imaging of photo-cross-linked small-molecule arrays on gold.
Identification of small-molecule ligands for a protein of interest can facilitate the analysis of the protein's functions in biological systems. Small-molecule microarrays have allowed for rapid detection of such ligand-protein interactions in a high-throughput manner, although a label on a protein is needed to observe these interactions. By combining SPR imaging technology with our recently developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ observation of interactions between photo-cross-linked small molecules on gold surfaces and nonlabeled proteins in solution. Interactions of estrogenic and androgenic substances with estrogen receptor alpha were observed using this platform.
Antibiotics Laboratory and Beam Application Team, Discovery Research Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. kanoh@riken.jp
Naoki
N
Kanoh
Motoki
M
Kyo
Kazuki
K
Inamori
Ami
A
Ando
Aya
A
Asami
Aiko
A
Nakao
Hiroyuki
H
Osada
D000728:Androgens / Q000378:metabolism; D001665:Binding Sites; D003432:Cross-Linking Reagents / Q000737:chemistry*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D008024:Ligands; D036103:Nanotechnology; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors
D000728:Androgens; D003432:Cross-Linking Reagents; D047628:Estrogen Receptor alpha; D004967:Estrogens; D008024:Ligands; D011506:Proteins; D006046:Gold