Comparative study between the Light Cycler and the PCR-restriction fragment length polymorphism in detecting factor V Leiden and factor II 20210G>A mutations.
OBJECTIVE
To test reproducibility, speed and cost of testing for factor V Leiden and FII 20210G>A in our practice.


METHODS
We compared conformity, reproducibility, speed and cost using the Light Cycler (LC) and PCR-RFLP.


RESULTS
There was 100% conformity and reproducibility. LC was faster but 23% more expensive per sample. When equipment depreciation and patient expenses are added, LC testing becomes cheaper.


CONCLUSIONS
In our practice, LC provides fast, reproducible and cost-effective results.
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, PO Box 3354, Riyadh 11211, Saudi Arabia. mammo@kfshrc.edu.sa
Layla
L
Mammo
Atia
A
Sheereen
Tanya
T
Saour
Jalal
J
Saour
D004252:DNA Mutational Analysis / Q000191:economics / Q000379:methods*; D005165:Factor V / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011516:Prothrombin / Q000235:genetics*; D015203:Reproducibility of Results
C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin