SPR imaging of photo-cross-linked small-molecule arrays on gold.
Identification of small-molecule ligands for a protein of interest can facilitate the analysis of the protein's functions in biological systems. Small-molecule microarrays have allowed for rapid detection of such ligand-protein interactions in a high-throughput manner, although a label on a protein is needed to observe these interactions. By combining SPR imaging technology with our recently developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ observation of interactions between photo-cross-linked small molecules on gold surfaces and nonlabeled proteins in solution. Interactions of estrogenic and androgenic substances with estrogen receptor alpha were observed using this platform.
Kanoh
Naoki
N
Antibiotics Laboratory and Beam Application Team, Discovery Research Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. kanoh@riken.jp
Kyo
Motoki
M
Inamori
Kazuki
K
Ando
Ami
A
Asami
Aya
A
Nakao
Aiko
A
Osada
Hiroyuki
H
D000728:Androgens / Q000378:metabolism; D001665:Binding Sites; D003432:Cross-Linking Reagents / Q000737:chemistry*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D008024:Ligands; D036103:Nanotechnology; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors
D000728:Androgens; D003432:Cross-Linking Reagents; D047628:Estrogen Receptor alpha; D004967:Estrogens; D008024:Ligands; D011506:Proteins; D006046:Gold