Alanine check points in HNN and HN(C)N spectra.
Rapid resonance assignment is a key requirement in structural genomics research by NMR. In this context we present here two new pulse sequences, namely, HNN-A and HN(C)N-A that have been developed by simple modification of the previously described pulse sequences, HNN and HN(C)N [S.C. Panchal, N.S. Bhavesh, R.V. Hosur, Improved 3D triple resonance experiments, HNN and HN(C)N, for H(N) and 15N sequential correlations in (13C, 15N) labeled proteins: application to unfolded proteins, J. Biomol. NMR, 20 (2001) 135-147]. These increase the number of start/check points in HNN and/or HN(C)N spectra and hence help in pacing up resonance assignment in proteins.
Chatterjee
Amarnath
A
Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India.
Kumar
Ashutosh
A
Hosur
Ramakrishna V
RV
D000409:Alanine / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry; D002247:Carbon Isotopes; D005998:Glycine / Q000737:chemistry; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011392:Proline / Q000737:chemistry; D017510:Protein Folding
D000596:Amino Acids; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011392:Proline; D000409:Alanine; D005998:Glycine