Pharmacokinetic study of orphenadrine using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).
We developed and validated a simple, rapid, and accurate HPLC-MS/MS method with simple protein precipitation for the determination of orphenadrine. Injection-to-injection running time was 3 min with a retention time of orphenadrine of 1.1 min. The linear assay range was 1-200 ng/mL (r2 > 0.99). The intra- and inter-assay imprecisions were CV 0.6-4.2% and CV 1.6-6.1%, respectively. The accuracy, extraction recovery, specificity and stability were satisfactory. Using the measured plasma concentrations of orphenadrine in 24 healthy subjects, pharmacokinetic profiles of orphenadrine were evaluated (AUC(0-72,) 1565+/-731 ng h/mL, Cmax 82.8+/-26.2 ng/mL, Tmax 3.0+/-0.9 h, elimination half-life 25.8+/-10.3 h).
Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea. suddenbz@smc.samsung.co.kr
Soo-Youn
SY
Lee
Hyeon Ju
HJ
Oh
Jong Won
JW
Kim
Yoon Gyoon
YG
Kim
Chul Jin
CJ
Moon
Eun Hee
EH
Lee
D000284:Administration, Oral; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006207:Half-Life; D006801:Humans; D013058:Mass Spectrometry; D009966:Orphenadrine / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D009966:Orphenadrine