Pentosidine in osteoarthritis: HPLC determination in body fluids and in tissues.
The aim of this study is to determine whether and to which extent pentosidine (PEN) is influenced in the case of advanced osteoarthritis (OA) in urine and in some tissues. Reverse phase HPLC method for PEN determination with fluorescence monitoring was utilized. Urinary pentosidine (U-PEN) and pyridinoline (U-PD) were determined in the group (N=67, age 67.1+/-11.7 years) in patients with OA before and after surgery (knee or hip replacement), and in healthy age-matched controls (N=18, age 67.0+/-6.0 years). Cartilage, synovial membrane and granulation tissue were also treated. In OA group significantly higher (P<0.001) U-PEN concentrations before (7.5+/-5.1 nmol/mmol creat.) and after (4.9+/-2.6 nmol/mmol creat.) surgery were found. U-PD was not significantly different between the groups (62.5+/-33.0 and 55.0+/-29.51 nmol/mmol creat., respectively), but differed significantly (P<0.0002 and P<0.01) from healthy subjects (40.2+/-16.5 nmol/mmol creat.). In the newly formed granulation tissue, PEN concentrations are significantly lower than in cartilage and in synovial tissues.
Institute of Rheumatology, Na Slupi 4, 128 50 Prague, Czech Republic. spacek@revma.cz
Pavel
P
Spacek
Milan
M
Adam
D000368:Aged; D000596:Amino Acids / Q000032:analysis / Q000652:urine; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D001826:Body Fluids / Q000737:chemistry*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003432:Cross-Linking Reagents / Q000032:analysis*; D005260:Female; D006097:Granulation Tissue / Q000737:chemistry*; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D013582:Synovial Fluid / Q000737:chemistry*
D000596:Amino Acids; D015415:Biomarkers; D003432:Cross-Linking Reagents; C015484:pyridinoline; D001120:Arginine; C062187:pentosidine; D008239:Lysine