DNA-programmed control of photosensitized singlet oxygen production.
DNA sequence-controlled on-and-off switching of a singlet oxygen sensitizer has been developed and demonstrated. The singlet oxygen photosensitizer pyropheophorbide-a (P) was attached to a 15-mer nucleotide sequence. A molecule that could quench the sensitizer, the so-called "black hole quencher 3" (Q), was attached to a complementary nucleotide strand. Upon hybridization of the two conjugates, singlet oxygen production from P was completely shut down. Upon the addition of a third DNA sequence that can displace and release the P-DNA conjugate from the P-Q pair, up to 85% of the singlet oxygen production was recovered. This system is a model for a benign drug that becomes active only in the presence of a specific targeted nucleotide sequence.
Department of Chemistry, University of Aarhus, Denmark.
Emiliano
E
Cló
John W
JW
Snyder
Niels V
NV
Voigt
Peter R
PR
Ogilby
Kurt V
KV
Gothelf
D002734:Chlorophyll / Q000031:analogs & derivatives / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D017319:Photosensitizing Agents / Q000737:chemistry*; D026082:Singlet Oxygen / Q000737:chemistry*
D009841:Oligonucleotides; D017319:Photosensitizing Agents; D002734:Chlorophyll; D026082:Singlet Oxygen; C040298:pyropheophorbide a; D004247:DNA