Eicosapentaenoic acid and 3,10 dithia stearic acid inhibit the desaturation of trans-vaccenic acid into cis-9, trans-11-conjugated linoleic acid through different pathways in Caco-2 and T84 cells.
Stearoyl-CoA desaturase (SCD) is a key enzyme that determines the composition and metabolic fate of ingested fatty acids, in particular the conversion of trans-vaccenic acid (TVA) to conjugated linoleic acid (CLA). The present study addressed the hypothesis that intestinal TVA absorption and biotransformation into CLA can be modulated by EPA and 3,10-dithia stearic acid (DSA) via altered SCD mRNA levels and desaturation indices (cis-9, trans-11-CLA:TVA and oleic acid:stearic acid ratios) in Caco-2 and T84 cells, two well-established in vitro models of the human intestinal epithelium. The study determined the effect of acute (3 h with 0.3 mm-EPA or 0.3 mm-DSA) and acute-on-chronic (1 week with 0.03 mm-EPA or -DSA, followed by respectively, 0.3 mm-EPA or -DSA for 3 h) treatments. In both cell lines, acute EPA treatment did not alter SCD desaturation indices, whereas the acute-on-chronic treatment affected these surrogate markers of SCD activity. This was associated with reduced sterol regulatory-element binding protein-1c and SCD mRNA levels. In contrast, acute and acute-on-chronic DSA treatments significantly reduced SCD desaturation indices without affecting SCD mRNA levels in Caco-2 cells. The present study on intestinal cells shows that the conversion rate of TVA to c9, t11-CLA is affected by other fatty acids present in the diet such as EPA, confirming previous observations in hepatic and mammary cell models.
Fisiologia Veterinaria e Nutrizione, Dept Science degli Alimenti, Università degli Studi di Udine, Italy.
Bénédicte
B
Renaville
Anne
A
Mullen
Fiona
F
Moloney
Yvan
Y
Larondelle
Yves-Jacques
YJ
Schneider
Helen M
HM
Roche
D018938:Caco-2 Cells; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004334:Drug Administration Schedule; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000378:metabolism*; D009829:Oleic Acids / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D013229:Stearic Acids / Q000494:pharmacology*; D013230:Stearoyl-CoA Desaturase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics
D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; D012333:RNA, Messenger; D013229:Stearic Acids; C503589:cis-9, trans-11-conjugated linoleic acid; C031183:stearic acid; D015118:Eicosapentaenoic Acid; D013230:Stearoyl-CoA Desaturase; C050413:11-octadecenoic acid