Down-regulation of the expression of beta1,4-galactosyltransferase V promotes integrin beta1 maturation.
In previous study, we have shown that beta1,4-galactosyltransferase V (GalT V) functions as a positive growth regulator in glioma. Here, we reported that down-regulation of the expression of GalT V in SHG44 cells by transfection with antisense cDNA specifically up-regulated the expression of cell surface integrin beta1 without the change of its mRNA, and with integrin beta1 125 kDa mature form increased and 105 kDa precursor form decreased. It is well known that the N-glycans of integrins modulate the location and functions of integrins. The SHG44 cells transfected with antisense cDNA of GalT V demonstrated decreased Golgi localization of integrin beta1, strengthened the interaction between integrin alpha5 and beta1 subunit, and enhanced the adhesion ability to fibronectin and the level of focal adhesion kinase phosphorylation. Our results suggested that the down-regulation of the expression of GalT V could promote the expression of cell surface integrin beta1 and subsequently inhibit glioma malignant phenotype.
Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Gene Research Center, Shanghai Medical College and Institutes of Biomedical Sciences of Fudan University, Shanghai 200032, PR China.
Xiaoning
X
Chen
Jianhai
J
Jiang
Junwu
J
Yang
Chun
C
Chen
Maoyun
M
Sun
Yuanyan
Y
Wei
Xiaoying
X
Guang
Jianxin
J
Gu
D001254:Astrocytoma / Q000201:enzymology* / Q000378:metabolism; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology; D015536:Down-Regulation; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D039482:Integrin alpha5 / Q000235:genetics / Q000378:metabolism; D019012:Integrin beta1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D039482:Integrin alpha5; D019012:Integrin beta1; D012333:RNA, Messenger; D005700:Galactosyltransferases; C419408:beta-1,4-galactosyltransferase V; D051416:Focal Adhesion Protein-Tyrosine Kinases