Recombinant hepatitis B large surface antigen, successfully produced in Escherichia coli, stimulates T-cell response in mice.
A fusion protein consisting of the PA binding domain of anthrax lethal factor (LFn) and a codon optimized Hepatitis B virus large surface antigen (LHBsAg) expresses well in Escherichia coli. The LFn-LHBsAg fusion protein effectively elicits a cell-mediated immune (CMI) response to the hepatitis B viral antigens in mice.
Shu
Li
L
Nankai University Vaccine Laboratory, Nankai University, Tianjin, China.
Touzjian
Neal
N
Nan
Deng
D
Kushner
Nicholas
N
Strong
Amie J
AJ
Zeping
Wei
W
Zhaohui
Geng
G
Lu
Yichen
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003062:Codon; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology*
D003062:Codon; D006514:Hepatitis B Surface Antigens; D011994:Recombinant Proteins