A simple and economical method for the manual construction of well-aligned tissue arrays.
Tissue array technology has allowed a substantial progression of studies correlating molecular and immunohistochemical findings with clinico-pathological information. Array construction presents technical difficulties and tissue arrayers are expensive, particularly for small and medium sized laboratories. We describe a simple manual method for producing well-aligned tissue arrays using a hand-made paper mold which can successfully perform immunohistochemical staining. All 200 tissue samples were collected and constructed into blocks by the paper mold. The tissue arrays were smoothly sectioned using a standard microtome and performed for a panel of immunohistochemical study with satisfactory results. This alterative method for building custom arrays could be applied in any laboratory and is both simple and economical.
Wang
Sheng-Lan
SL
Department of Pathology, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan.
Yang
Ching-Hsiu
CH
Chen
Hsin-Hung
HH
Chai
Chee-Yin
CY
D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D010340:Pathology, Surgical / Q000295:instrumentation / Q000379:methods*; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*; D046888:Tissue Array Analysis / Q000295:instrumentation / Q000379:methods*