Horseradish peroxidase-driven fluorescent labeling of nanotubes with quantum dots.
We describe the first enzyme-driven technique for fluorescent labeling of single-walled carbon nanotubes (SWNTs). The labeling was performed via enzymatic biotinylation of nanotubes in the tyramide-horseradish peroxidase (HRP) reaction. Both direct and indirect fuorescent labeling of SWNTs was achieved using either biotinyl tyramide or fluorescently tagged tyramides. Biotinylated SWNTs later reacted with streptavidin-conjugated fluorophores. Linking semiconductor nanocrystals, quantum dots (Q-dots), to the surface of nanotubes resulted in their fluorescent visualization, whereas conventional fluorophores bound to SWNTs directly or through biotin-streptavidin linkage, were completely quenched. Enzymatic biotinylation permits fluorescent visualization of carbon nanotubes, which could be useful for a number of biomedical applications. In addition, other organic molecules such as proteins, antibodies, or DNA can be conjugated to biotinylated SWNTs using this approach.
Didenko
Vladimir V
VV
Baylor College of Medicine, Houston, TX, USA. vdidenko@bcm.tmc.edu
Baskin
David S
DS
D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure*; D045663:Quantum Dots; D013194:Staining and Labeling / Q000379:methods
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase