Is photobleaching necessary for Raman imaging of bone tissue using a green laser?
Raman microspectroscopy is widely used for musculoskeletal tissues studies. But the fluorescence background obscures prominent Raman bands of mineral and matrix components of bone tissue. A 532-nm laser irradiation has been used efficiently to remove the fluorescence background from Raman spectra of cortical bone. Photochemical bleaching reduces over 80% of the fluorescence background after 2 h and is found to be nondestructive within 40 min. The use of electron multiplying couple charge detector (EMCCD) enables to acquire Raman spectra of bone tissues within 1-5 s range and to obtain Raman images less than in 10 min.
Golcuk
Kurtulus
K
Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
Mandair
Gurjit S
GS
Callender
Andrew F
AF
Sahar
Nadder
N
Kohn
David H
DH
Morris
Michael D
MD
D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D003952:Diagnostic Imaging / Q000379:methods*; D005453:Fluorescence; D036681:Fluorescence Recovery After Photobleaching; D007834:Lasers; D008903:Minerals / Q000032:analysis; D038761:Photobleaching; D013059:Spectrum Analysis, Raman / Q000379:methods*
D008903:Minerals