Stabilization of free and immobilized enzymes using hyperthermophilic chaperonin.
Chaperonins suppress the denaturation of proteins and promote protein folding in vivo. Because hyperthermophilic chaperonins are expected to be used as a stabilizer for proteins, the effects of a group II chaperonin from a hyperthermophilic archaeum, Thermococcus strain KS-1 (T. KS-1 cpn), on the stabilization of mesophilic and thermophilic free enzymes and an enzyme co-immobilized with T. KS-1 cpn were studied. T. KS-1 cpn prevented the thermal inactivation of yeast alcohol dehydrogenase (ADH), jack bean urease, and Thermus flavus malate dehydrogenase (MDH) at high temperatures. T. KS-1 cpn also improved the long-term stability of ADH at lower temperatures. Moreover, the residual ADH activity of ADH co-entrapped with T. KS-1 cpn was improved and maintained at a higher level than that of the entrapped ADH without chaperonin. T. KS-1 cpn is useful for the stabilization of free and immobilized enzymes and applicable to various fields of biotechnology.
Department of Information Machines and Interfaces, Faculty of Information Science, Hiroshima City University, 3-4-1 Ozuka-higashi, Hiroshima 731-3194, Japan. kohda@bio.im.hiroshima-cu.ac.jp
Jiro
J
Kohda
Hirofumi
H
Kawanishi
Ken-Ichiro
K
Suehara
Yasuhisa
Y
Nakano
Takuo
T
Yano
D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D018833:Chaperonins / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005782:Gels; D006358:Hot Temperature; D008291:Malate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D019712:Thermococcus / Q000235:genetics; D014510:Urease / Q000737:chemistry / Q000378:metabolism
D004800:Enzymes, Immobilized; D005782:Gels; D011994:Recombinant Proteins; D000426:Alcohol Dehydrogenase; D008291:Malate Dehydrogenase; D014510:Urease; D018833:Chaperonins