A novel method for glucose determination based on electrochemical impedance spectroscopy using glucose oxidase self-assembled biosensor.
A method is developed for quantitative determination of glucose using electrochemical impedance spectroscopy (EIS). The method is based on immobilized glucose oxidase (GOx) on the topside of gold mercaptopropionic acid self-assembled monolayers (Au-MPA-GOx SAMs) electrode and mediation of electron transfer by parabenzoquinone (PBQ). The PBQ is reduced to hydroquinone (H(2)Q), which in turn is oxidized at Au electrode in diffusion layer. An increase in the glucose concentration results in an increase in the diffusion current density of the H(2)Q oxidation, which corresponds to a decrease in the faradaic charge transfer resistance (R(ct)) obtained from the EIS measurements. Glucose is quantified from linear variation of the sensor response (1/R(ct)) as a function of glucose concentration in solution. The method is straightforward and nondestructive. The dynamic range for determination of glucose is extended to more than two orders of magnitude. A detection limit of 15.6 microM with a sensitivity of 9.66 x 10(-7) Omega(-1)mM(-1) is obtained.
Chemistry Department, University of Isfahan, Isfahan 81746-73441, IR Iran. rkarimi@sci.ui.ac.ir
Reza Karimi
RK
Shervedani
Abdolhamid Hatefi
AH
Mehrjardi
Najmehsadat
N
Zamiri
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods
D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D005947:Glucose