Proteomics-based analysis of a pair of glioma cell lines with different tumor forming characteristics.
Glioma is the most common malignant disease in the brain, and recurrence is the main cause of death from this disease. Tumor recurrence involves multiple steps, and requires the accumulation of the altered expression of many different proteins. Identification of the recurrence associated protein profile in glioma cell lines will be helpful in clarifying the molecular mechanisms underlying glioma recurrence. In this report, two glioma cell lines with distinct tumor forming ability in vitro and in vivo were chosen and the different protein expression patterns were analyzed by proteomics method. To confirm the utility of this method, we validated the differential expression of one protein, cathepsin D, by immunohistochemistry analysis. Forty-six proteins appeared differently between two cell lines and 18 of them were identified. These 18 are involved in cell proliferation, DNA replication, protein synthesis, invasion, angiogenesis and neurotrophic factor. All of these molecules are important in tumor growth, and a subset of them may be related to glioma recurrence. These findings may contribute to the discovery of new diagnostic markers and therapeutic targets of glioma.
Zhou
Le
L
Institute for Cancer Research, The Key Laboratory of Biomedical Information Engineering of Chinese Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710061, China.
Wang
Yan
Y
Zhang
Ying-Tao
YT
Geng
Yi-Ping
YP
Si
Lu-Sheng
LS
Wang
Yi-Li
YL
D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D002402:Cathepsin D / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004261:DNA Replication / Q000502:physiology; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D007150:Immunohistochemistry; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000503:physiopathology; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D040901:Proteomics / Q000379:methods* / Q000639:trends
D014408:Biomarkers, Tumor; D002402:Cathepsin D