Characterization of a gene encoding an acetylase required for pyoverdine synthesis in Pseudomonas aeruginosa.
Strains of Pseudomonas aeruginosa secrete one of three pyoverdine siderophores (types I to III). We have characterized a gene, pvdY(II) (for the pvdY gene present in type II P. aeruginosa strains), that is only present in strains that make type II pyoverdine. A mutation in pvdY(II) prevented pyoverdine synthesis. Bioinformatic, genetic, and biochemical approaches indicate that the PvdYII enzyme catalyzes acetylation of hydroxyornithine. Expression of pvdY(II) is repressed by the presence of iron and upregulated by the presence of type II pyoverdine. Characterization of pvdY(II) provides insights into the molecular basis for production of different pyoverdines by different strains of P. aeruginosa.
Lamont
Iain L
IL
Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand. iain.lamont@stonebow.otago.ac.nz.
Martin
Lois W
LW
Sims
Talia
T
Scott
Amy
A
Wallace
Mary
M
D000115:Acetylesterase / Q000235:genetics* / Q000302:isolation & purification; D019295:Computational Biology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D005786:Gene Expression Regulation; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D005798:Genes, Bacterial; D007501:Iron / Q000494:pharmacology; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009842:Oligopeptides / Q000096:biosynthesis* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D026801:Synteny
D004269:DNA, Bacterial; D009842:Oligopeptides; C042453:pyoverdin; D007501:Iron; D000115:Acetylesterase