Application of L-Amino acid in determination of Huperzine A by high performance liquid chromatography.
A selective HPLC method for determination of Huperzine A in Huperzia serrata Extract has been developed and validated. Huperzine A was dissolved in 0.01 mol/L HCl, chromatographed on an Agilent Zorbax SB-C18 (150 mm x 4.6 mm i.d., 5 microm) column, with a mobile phase consisting of methanol-1mM L-Lysine water solution (50:50, v/v), and detected at 310 nm. The UV peak areas were used for quantitation of Huperzine A content. The correlation coefficient (R(2)) of the calibration was 0.9999 over the range of 1-25 microg/ml and intra- and interday precision over this range were not more than 2%. The method was successfully applied to characterize and determine the Huperzine A in Huperzia serrata Extract.
Key Laboratory of Bio-process, Hefei University of Technology, Ministry of Education, Hefei, Anhui 230009, PR China.
Jian
J
Pan
Risheng
R
Jin
Xueqiao
X
Hu
D000470:Alkaloids; D000596:Amino Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000470:Alkaloids; D000596:Amino Acids; D012717:Sesquiterpenes; C050426:huperzine A