Delineation of Borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of Borrelia spielmanii sp. nov.
To evaluate multilocus sequence analysis (MLSA) for taxonomic purposes in the delineation of species within Borrelia burgdorferi sensu lato, seven relevant loci of various strains for which extensive DNA-DNA reassociation data were available were sequenced. MLSA delineation proved to be fully concordant with conventional methods. Our analysis confirmed the delineation of a novel species, Borrelia spielmanii sp. nov., previously known as 'Borrelia spielmani' Richter et al. 2004, with strain PC-Eq17N5T (=DSM 16813T = CIP 108855T) as the type strain.
Charité Universitätsmedizin Berlin, 12249 Berlin, Germany.
Dania
D
Richter
Institut Pasteur, 75724 Paris, France.
Danièle
D
Postic
Institut Pasteur, 75724 Paris, France.
Natacha
N
Sertour
Baxter Vaccine AG, A-2304 Orth/Donau, Austria.
Ian
I
Livey
Charité Universitätsmedizin Berlin, 12249 Berlin, Germany.
Franz-Rainer
FR
Matuschka
Institut Pasteur, 75724 Paris, France.
Guy
G
Baranton
D015373:Bacterial Typing Techniques; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry*; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
D004269:DNA, Bacterial