Bionanofabrication of metallic and semiconductor nanoparticle arrays using S-layer protein lattices with different lateral spacings and geometries.
Two-dimensional (2-D) surface layer (S-layer) protein lattices isolated from the gram-positive bacterium Deinococcus radiodurans and the acidothermophilic archaeon Sulfolobus acidocaldarius were investigated and compared for their ability to biotemplate the formation of self-assembled, ordered arrays of inorganic nanoparticles (NPs). The NPs employed for these studies included citrate-capped gold NPs and various species of CdSe/ZnS core/shell quantum dots (QDs). The QD nanocrystals were functionalized with different types of thiol ligands (negative- or positive-charged/short- or long-chain length) in order to render them hydrophilic and thus water-soluble. Transmission electron microscopy, Fourier transform analyses, and pair correlation function calculations revealed that ordered nanostructured arrays with a range of spacings (approximately 7-22 nm) and different geometrical arrangements could be fabricated through the use of the two types of S-layers. These results demonstrate that it is possible to exploit the physicochemical/structural diversity of prokaryotic S-layer scaffolds to vary the morphological patterning of nanoscale metallic and semiconductor NP arrays.
Department of Microbiology, Cornell University, Ithaca, New York 14853, USA. ssm12@cornell.edu
Sonny S
SS
Mark
Magnus
M
Bergkvist
Xin
X
Yang
Leonardo M
LM
Teixeira
Parijat
P
Bhatnagar
Esther R
ER
Angert
Carl A
CA
Batt
D019187:Cadmium Compounds / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods; D019343:Citric Acid / Q000737:chemistry; D034301:Deinococcus / Q000378:metabolism; D005583:Fourier Analysis; D008670:Metals / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D018036:Selenium Compounds / Q000737:chemistry; D012666:Semiconductors; D013440:Sulfides / Q000737:chemistry; D016945:Sulfolobus acidocaldarius / Q000378:metabolism; D013499:Surface Properties; D014867:Water / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
D019187:Cadmium Compounds; D008670:Metals; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D014867:Water; D019343:Citric Acid; C058667:cadmium selenide; C031238:zinc sulfide