Molecular cloning, characterization and expression of a novel trehalose-6-phosphate synthase homologue from Ginkgo biloba.
In many organisms, trehalose acts as protective metabolite against harsh environmental stresses, such as freezing, drought, nutrient starvation, heat and salt. Herein a cDNA (designated as GbTPS, GenBank Accession Number AY884150) encoding a trehalose-6-phosphate synthase homologue was isolated and characterized from the living fossil plant, Ginkgo biloba, which is highly tolerant to drought and cold. GbTPS encoded an 868-amino-acid polypeptide with a predicted isoelectric point of 5.83 and molecular mass of 97.9 kD. Amino acid sequence alignment revealed that GbTPS shared high identity with class II trehalose-6-phosphate synthase homologues (67% identical to AtTPS7), but had only 17% and 23% of identity with OstA from Escherichia coli and ScTPS1 from S. cerevisiae, respectively. DNA gel blot analysis indicated that GbTPS belonged to a small multi-gene family. The expression analysis by RT-PCR showed that GbTPS expressed in a tissue-specific manner in G. biloba and might involve in leaf development. GbTPS was also found to be induced by a variety of stresses including cold, salt, drought and mannitol.
Wu
Weisheng
W
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, China.
Pang
Yongzhen
Y
Shen
Guo-an
GA
Lu
Jie
J
Lin
Juan
J
Wang
Jin
J
Sun
Xiaofen
X
Tang
Kexuan
K
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000737:chemistry; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D020441:Ginkgo biloba / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D005964:Glucosyltransferases / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics*; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology
D018076:DNA, Complementary; D008353:Mannitol; D012965:Sodium Chloride; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase