Two new chiral EDTA-based metal chelates for weak alignment of proteins in solution.
[structure: see text] A short synthesis of EDTA-based metal chelates that can be attached to the cysteine residue of a protein via a disulfide bond is described. The complexes were used after coordination of lanthanides to align trigger factor and apo-calmodulin in solution to yield residual dipolar couplings and pseudocontact shifts. Alignment tensors for the new tags are linearly independent compared to those of previously published tags.
Haberz
Peter
P
Max-Planck Institute for Biophysical Chemistry, Department of NMR-Based Structural Biology, Am Fassberg 11, 37077 Göttingen, Germany.
Rodriguez-Castañeda
Fernando
F
Junker
Jochen
J
Becker
Stefan
S
Leonov
Andrei
A
Griesinger
Christian
C
D002147:Calmodulin / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D004492:Edetic Acid; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D012996:Solutions
D002147:Calmodulin; D002614:Chelating Agents; D028581:Lanthanoid Series Elements; D011506:Proteins; D012996:Solutions; D004492:Edetic Acid; D003545:Cysteine