Biosynthesis of cytokinins in cytokinin-autotrophic tobacco callus.
A cytokinin-autotrophic strain of tobacco callus contained cytokinin-active compounds with chromatographic mobilities on Sephadex LH-20 corresponding to ribosylzeatin, zeatin, and 6-Delta(2)-isopentenylaminopurine. Zeatin, the apparent major cytokinin, was estimated to be present at a concentration of 10(-4) mumol/kg of tissue. Cytokinin-autotrophic callus supplied with [(14)C]adenine produced radioactive components with the same chromatographic properties as zeatin and 6-Delta(2)-isopentenylaminopurine. These components were not obtained from cytokinin-dependent tissue supplied with [(14)C]adenine in the same manner.
Institute of Plant Development, Birge Hall, University of Wisconsin, Madison, Wis. 53706.
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JW
Einset
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Skoog