SGK protein kinase facilitates the expression of long-term potentiation in hippocampal neurons.
Previous studies showed that the serum- and glucocorticoid-inducible kinase (sgk) gene plays an important role in long-term memory formation. The present study further examined the role of SGK in long-term potentiation (LTP). The dominant-negative mutant of sgk, SGKS422A, was used to inactivate SGK. Results revealed a time-dependent increase in SGK phosphorylation after tetanization with a significant effect observed 3 h and 5 h later. Transfection of SGKS422A impaired the expression, but not the induction, of LTP. Furthermore, the constitutively active sgk, SGKS422D, up-regulated postsynaptic density-95 expression in the hippocampus. These results together support the role of SGK in neuronal plasticity.
Division of Neuroscience, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.
Yun L
YL
Ma
Ming C
MC
Tsai
Wei L
WL
Hsu
Eminy H Y
EH
Lee
D000818:Animals; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D017874:Immediate-Early Proteins / Q000502:physiology*; D017774:Long-Term Potentiation / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D051381:Rats; D011922:Rats, Mutant Strains; D009435:Synaptic Transmission / Q000502:physiology; D013997:Time Factors; D014162:Transfection; D000099184:Serum-Glucocorticoid Regulated Kinases
D017874:Immediate-Early Proteins; D017346:Protein Serine-Threonine Kinases; D000099184:Serum-Glucocorticoid Regulated Kinases