Profiling urinary metabolites of naproxen by liquid chromatography-electrospray mass spectrometry.
Glucuronidation, an important metabolic process for the biotransformation of drugs into easily eliminable water-soluble detoxification products, can also lead to biologically active or toxic glucuronide conjugates. The present work describes a liquid chromatography-electrospray mass spectrometry (LC-ESI-MS) approach for the characterization of naproxen and O-6-desmethylnaproxen glucuronides. The method is fast and efficient and permitted to individuate alpha and beta isomers of both naproxen and O-6-desmethylnaproxen glucuronides. The procedure could be potentially extended to the characterization of other drug metabolites.
Aresta
Antonella
A
Centro Interdipartimentale di Ricerca "Spettrometria di Massa Analitica per Ricerche Tecnologiche (SMART)" Università degli Studi di Bari, Dipartimento di Chimica, Via Orabona 4, 70126-BARI, Italy.
Carbonara
Teresa
T
Palmisano
Francesco
F
Zambonin
Carlo G
CG
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism* / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods*; D020719:Glucuronides / Q000652:urine; D006801:Humans; D009288:Naproxen / Q000031:analogs & derivatives / Q000378:metabolism* / Q000652:urine; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D020719:Glucuronides; C430408:naproxen glucuronide; D009288:Naproxen