Molecular characterization of pestiviruses isolated from bovines in Chile.
Thirty-three pestiviruses isolated from bovines on different farms in Chile were characterized at the molecular level. The 5'-untranslated region (5'UTR) of the isolates was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and subsequently digested with restriction enzymes (RE) Bgl I, Xho I and Pst I. Furthermore, the isolates were amplified by differential RT-PCR, which selectively amplified bovine viral diarrhea virus type 2 (BVDV-2) but not bovine viral diarrhea virus type 1 (BVDV-1). Of the 33 isolates, 16 were classified as BVDV-1 and 17 as BVDV-2. Phylogenetic analysis of the PCR-amplified fragments from 14 isolates showed the presence of viruses belonging to the BVDV-1a, BVDV-1b, BVDV-1c, and BVDV-2 types. Antigenicity of some viruses belonging to both genotypes was studied by cross-seroneutralization, revealing great antigenic differences among them. It is concluded that BVDV viruses circulating in Chile are genetically and antigenically heterogeneous, comprising isolates of genogroups 1 and 2.
Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, Chile. jpizarro@uchile.cl
José
J
Pizarro-Lucero
María-Orfelia
MO
Celedón
Marcela
M
Aguilera
Alejandra
A
de Calisto
D000818:Animals; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology / Q000382:microbiology*; D002417:Cattle; D002677:Chile / Q000453:epidemiology; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005784:Gene Amplification; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary