Selective transmission of hepatitis C virus quasi species through a needlestick accident in acute resolving hepatitis.
BACKGROUND
Little is known about the transmission of variant hepatitis C virus (HCV) genome through needlestick injuries.


METHODS
To demonstrate how HCV quasi species are transmitted and adapt to the new host in acute resolving infection, we analyzed the nucleotide and deduced amino acid sequences of the hypervariable region 1 (HVR-1) in the E2 domain of HCV in both the source of the virus ("donor") and the person who received the virus through a needlestick accident ("recipient"). In addition, we also performed phylogenetic analysis of HCV quasi species in these patients to document the viral transmission.


RESULTS
We obtained a total of 33 clones at different time points by using polymerase chain reaction amplification and cloning and sequencing of HVR-1. A predominant HVR-1 variant (in 4 of 10 isolates) in the donor was not present in the recipient 6 and 14 weeks after the accident. In contrast, a minor variant (in 1 of 10 isolates) in the donor became the predominant strain in the recipient 6 weeks (in 10 of 12 isolates) and 14 weeks (in 6 of 11 isolates) after the accident. Additional phylogenetic analysis showed high homology of nucleotide sequences between isolates obtained from the donor and isolates obtained from the recipient. In addition, the variants in the recipient's virus showed substantial genetic preservation in the course of acute resolving hepatitis.


CONCLUSIONS
These data suggested that a minor HCV variant from a donor was transmitted to the recipient through a needlestick injury and that it prevailed as the dominant species. The preserved genetic homogeneity of the transmitted viral variants in patients with acute HCV infection may account for their clinical outcomes of resolving hepatitis.
Liu
Chen-Hua
CH
Department of Internal Medicine, National Taiwan University Hospital, Yun-Lin Branch, Taiwan.
Chen
Bing-Fang
BF
Chen
Shu-Ching
SC
Lai
Ming-Yang
MY
Kao
Jia-Horng
JH
Chen
Ding-Shinn
DS
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D005260:Female; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000635:transmission*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016602:Needlestick Injuries / Q000821:virology*; D010802:Phylogeny; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
C400562:HVR1 protein, Hepatitis C virus; D014764:Viral Proteins