Differential gene expression profiles in the hippocampus of senescence-accelerated mouse.
The senescence-accelerated mouse (SAM) is an animal model for studying senescence and age-associated disorders due to its inherited aging phenotype. The SAM/prone8 (SAMP8) is a useful animal model to investigate the fundamental mechanisms involved in age-related learning and memory deficits that may have relevance to age-associated AD, while SAM/resistant1 (SAMR1) shows normal. To identify genes rendering the cognitive deterioration with aging, the subtractive cDNA libraries containing 1924 clones with the positive ratio of 96.18% were generated and the microarray containing 3136 cDNA was prepared. The results of screening libraries by the microarray showed that of all 91 differentially expressed genes, 50 were over-expressed and 41 were low-expressed in SAMP8. Some of the identified genes were confirmed by the real time quantitative RT-PCR. These results indicated the profiles of gene expression in the hippocampus of SAMP8 and SAMR1 were significantly different, which may play important roles in the age-related cognitive deficit in SAMP8, suggesting those genes related to the cognitive deficient or pathology change in the brain of SAMP8 may be potential gene targets for Alzheimer's disease therapy.
Cheng
Xiao-Rui
XR
Beijing Institute of Pharmacology and Toxicology, Beijing 100850, PR China.
Zhou
Wen-Xia
WX
Zhang
Yong-Xiang
YX
Zhou
Dong-Sheng
DS
Yang
Rui-Fu
RF
Chen
Ling-Feng
LF
D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D015723:Gene Library; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D012333:RNA, Messenger