Efficient screening of the cystinuria-related C663T Slc3a1 nonsense mutation in Newfoundland dogs by denaturing high-performance liquid chromatography.
Cystinuria in Newfoundland dogs is a metabolic disease associated with a nonsense mutation in the exon 2 of the Slc3a1 gene. Similar to type I human cystinuria, heterozygote carriers are not affected by the disease and do not reveal differences in urinary concentration of dibasic amino acids when compared with normal dogs. However, through a recessive mode of inheritance, these dogs are able to transmit the disease to their offspring. Early detection of mutation carriers through cost-effective reliable methods is therefore essential for the implementation of breeding methods aimed at the eradication of the disease. Denaturing high-performance liquid chromatography (DHPLC) is a recently developed technique for rapid and efficient screening of nucleotide polymorphisms in polymerase chain reaction-amplified products. This technique was used for the identification of the C663T Slc3a1 mutation in Portuguese Newfoundland dogs. Polymerase chain reaction products amplified from a region containing the C663T locus were subjected to DHPLC analysis, and results were double checked by DNA sequencing. Results showed the presence of the mutation in 6 of the 22 dogs tested. Urine biochemical parameters correlated well with the number of mutated Slc3a1 copies, and homozygotes for the C663T mutation were the only dogs diagnosed with cystinuria. Sequence analysis confirmed the DHPLC results, demonstrating that the technique could be a reliable alternative to sequencing for the rapid and cost-effective identification of mutations in canine breeds.
Matos
Augusto José Ferreira
AJ
Institute for the Biomedical Sciences of Abel Salazar, University of Porto, Portugal.
Mascarenhas
Cláudia
C
Magalhães
Paula
P
Pinto
Jorge Pereira
JP
D026905:Amino Acid Transport Systems / Q000235:genetics*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000662:veterinary*; D018389:Codon, Nonsense; D003555:Cystinuria / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D017931:DNA Primers / Q000235:genetics; D004283:Dog Diseases / Q000175:diagnosis* / Q000235:genetics; D004285:Dogs; D005260:Female; D008297:Male; D008403:Mass Screening; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011174:Portugal
D026905:Amino Acid Transport Systems; D018389:Codon, Nonsense; D017931:DNA Primers