Differential gene expression in Perillus bioculatus nymphs fed a suboptimal artificial diet.
Fragments of two artificial diet up-regulated and two prey up-regulated transcripts were isolated from the predatory Pentatomid Perillus bioculatus using suppression subtractive hybridization. A BlastX search found similarities for two diet-upregulated clones, i.e., the tyrosine-3-monooxygenase gene and the gene for the chitin binding protein, Gasp. The probe generated from the tyrosine-3-monooxygenase clone hybridized to two transcripts 2.3 and 1.2kb in size. The two transcripts were differentially regulated: the 2.3kb transcript was upregulated in the first and late third instar diet-fed nymphs, whereas the 1.2kb transcript was upregulated in the second and early third instar diet-fed nymphs. The Gasp gene was upregulated in late third instar nymphs. A positive correlation was found between levels of expression of the isolated genes and the number of generations the insects had been reared on the artificial diet.
Yocum
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GD
Biosciences Research Laboratory, USDA-ARS Red River Valley Agricultural Research Center, 1605 Albrecht Boulevard, Fargo, ND 58105, USA. yocumg@fargo.ars.usda.gov
Coudron
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TA
Brandt
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SL
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D015415:Biomarkers; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017344:Genes, Insect; D020062:Heteroptera / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D009758:Nymph / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D015415:Biomarkers