Cellular factors required for Lassa virus budding.
It is known that Lassa virus Z protein is sufficient for the release of virus-like particles (VLPs) and that it has two L domains, PTAP and PPPY, in its C terminus. However, little is known about the cellular factor for Lassa virus budding. We examined which cellular factors are used in Lassa virus Z budding. We demonstrated that Lassa Z protein efficiently produces VLPs and uses cellular factors, Vps4A, Vps4B, and Tsg101, in budding, suggesting that Lassa virus budding uses the multivesicular body pathway functionally. Our data may provide a clue to develop an effective antiviral strategy for Lassa virus.
First Department of Forensic Science, National Research Institute of Police Science, Kashiwa 277-0882, Japan.
Shuzo
S
Urata
Takeshi
T
Noda
Yoshihiro
Y
Kawaoka
Hideyoshi
H
Yokosawa
Jiro
J
Yasuda
D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000502:physiology; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000502:physiology; D056827:Endosomal Sorting Complexes Required for Transport; D007836:Lassa virus / Q000502:physiology*; D000075702:Nedd4 Ubiquitin Protein Ligases; D034741:RNA, Small Interfering / Q000494:pharmacology; D012097:Repressor Proteins / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology; D014162:Transfection; D044767:Ubiquitin-Protein Ligases / Q000502:physiology; D025262:Vacuolar Proton-Translocating ATPases; D033921:Vesicular Transport Proteins; D014771:Virion / Q000502:physiology; D000099177:Tsg101 Protein
D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases; D004268:DNA-Binding Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D000075702:Nedd4 Ubiquitin Protein Ligases; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D014157:Transcription Factors; D044767:Ubiquitin-Protein Ligases; D025262:Vacuolar Proton-Translocating ATPases; D033921:Vesicular Transport Proteins; D000099177:Tsg101 Protein; C490305:VPS4A protein, human; C490304:VPS4B protein, human