Decreased CD90 expression in human mesenchymal stem cells by applying mechanical stimulation.
BACKGROUND
Mesenchymal stem cells (MSC) are multipotent cells which can differentiate along osteogenic, chondrogenic, and adipogenic lineages. The present study was designed to investigate the influence of mechanical force as a specific physiological stress on the differentiation of (MSC) to osteoblast-like cells.


METHODS
Human MSC were cultured in osteoinductive medium with or without cyclic uniaxial mechanical stimulation (2000 mustrain, 200 cycles per day, 1 Hz). Cultured cells were analysed for expression of collagen type I, osteocalcin, osteonectin, and CD90. To evaluate the biomineral formation the content of bound calcium in the cultures was determined.


RESULTS
After 14 days in culture immunfluorescence staining revealed enhancement of collagen type I and osteonectin expression in response to mechanical stimulation. In contrast, mechanically stimulated cultures stained negative for CD90. In stimulated and unstimulated cultures an increase in the calcium content over time was observed. After 21 days in culture the calcium content in mechanical stimulated cultures was significantly higher compared to unstimulated control cultures.


CONCLUSIONS
These results demonstrate the influence of mechanical force on the differentiation of human MSC into osteoblast-like cells in vitro. While significant enhancement of the biomineral formation by mechanical stimulation is not detected before 21 days, effects on the extracellular matrix became already obvious after 14 days. The decrease of CD90 expression in mechanically stimulated cultures compared to unstimulated control cultures suggests that CD90 is only transiently expressed expression during the differentiation of MSC to osteoblast-like cells in culture.
Wiesmann
Anne
A
Medizinische Klinik und Poliklinik, Abteilung für Hämatologie, Onkologie, Immunologie und Rheumatologie, Universitätsklinikum Tübingen, Tübingen, Germany. anne.wiesmann@med.uni-tuebingen.de
Bühring
Hans-Jörg
HJ
Mentrup
Christoph
C
Wiesmann
Hans-Peter
HP