Oceanisphaera donghaensis sp. nov., a halophilic bacterium from the East Sea, Korea.
A taxonomic study was carried out on two isolates, strains BL1(T) and BL11, from marine sediment collected from the East Sea, Korea. Comparative 16S rRNA gene sequence studies showed that these isolates clearly affiliated with the Gammaproteobacteria. BL1T and BL11 were most closely related to Oceanisphaera litoralis KMM 3654T (97.6 and 97.7 % 16S rRNA gene sequence similarity, respectively). The level of 16S rRNA gene sequence similarity between strains BL1T and BL11 was 99.7 %. The two isolates were Gram-negative, aerobic, moderately halophilic, and grew in 0.5-8.0 % NaCl and at 4-42 degrees C. Strains BL1T and BL11 shared some physiological and biochemical properties with O. litoralis KMM 3654T, although they differed in that BL1T and BL11 were able to utilize ethanol, proline and alanine. The G+C contents of the genomic DNA of strains BL1T and BL11 were 56.6 and 57.1 mol%, respectively. Both strains possessed C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH, C(16 : 0) and C(18 : 1)omega7c as the major fatty acids. DNA-DNA relatedness data indicated that strains BL1T and BL11 represent a genomic species that is separate from O. litoralis KMM 3654T. On the basis of polyphasic evidence, it is proposed that strain BL1T (=KCTC 12522T = DSM 17589T) represents the type strain of a novel species, Oceanisphaera donghaensis sp. nov.
Park
Soo-Je
SJ
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, 12 Gaeshin-dong Cheongju 361-763, Republic of Korea.
Kang
Cheol-Hee
CH
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, 12 Gaeshin-dong Cheongju 361-763, Republic of Korea.
Nam
Young-Do
YD
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eundong 52, Yusong, Daejon 305-600, Republic of Korea.
Bae
Jin-Woo
JW
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eundong 52, Yusong, Daejon 305-600, Republic of Korea.
Park
Yong-Ha
YH
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eundong 52, Yusong, Daejon 305-600, Republic of Korea.
Quan
Zhe-Xue
ZX
Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China.
Moon
Deok-Soo
DS
Deep Ocean Water Application Research Center, Korea Ocean Research and Development Institute (KORDI), 245-7 Oho-ri, Jugwang-myeon, Goseong-gun, Gangwon-do, 219-822, Republic of Korea.
Kim
Hyeon-Ju
HJ
Deep Ocean Water Application Research Center, Korea Ocean Research and Development Institute (KORDI), 245-7 Oho-ri, Jugwang-myeon, Goseong-gun, Gangwon-do, 219-822, Republic of Korea.
Roh
Dong-Hyun
DH
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, 12 Gaeshin-dong Cheongju 361-763, Republic of Korea.
Rhee
Sung-Keun
SK
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, 12 Gaeshin-dong Cheongju 361-763, Republic of Korea.
D044044:Aeromonadaceae / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D005227:Fatty Acids / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride