Microdevice DNA forensics by the simple tandem repeat method.
We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Full 16-locus multiplexes are verified on the multilane system. Multi-donor mixed samples are studied in the context of the limits of the laser-induced fluorescence detector and data-reduction software. The microdevice appears to be posed to outperform current capillary arrays in terms of stability and, through specialized sample loading, in the interpretation of complex mixtures.
Goedecke
Nils
N
Whitehead Institute for Biomedical Research, Nine Cambridge Center, MA 02142-1479, USA.
McKenna
Brian
B
El-Difrawy
Sameh
S
Gismondi
Elizabeth
E
Swenson
Abigail
A
Carey
Loucinda
L
Matsudaira
Paul
P
Ehrlich
Daniel J
DJ
D004247:DNA / Q000235:genetics*; D005554:Forensic Medicine; D006801:Humans; D020080:Tandem Repeat Sequences
D004247:DNA