Identification of novel ARF binding proteins by two-hybrid screening.
The ARF tumor suppressor protects us against cancer through protein-protein interactions in partially defined p53-dependent and p53-independent pathways. We performed a two-hybrid screen using ARF as bait and present the identification of several new ARF partners that may regulate its growth inhibitory signaling. The potential physiological roles of these novel ARF binding proteins in regulating ARF signaling are discussed.
Tompkins
Van
V
Department of Pharmacology, The University of Iowa, College of Medicine, Iowa City 52242, USA.
Hagen
Jussara
J
Zediak
Valerie P
VP
Quelle
Dawn E
DE
D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D002522:Chlorocebus aethiops; D019941:Cyclin-Dependent Kinase Inhibitor p16; D006131:Growth Inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D011485:Protein Binding / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; C417743:Cdkn2a protein, mouse; D019941:Cyclin-Dependent Kinase Inhibitor p16; D006131:Growth Inhibitors; D025803:Tumor Suppressor Protein p14ARF