Isolation and genetic mapping of a Coffea canephora phenylalanine ammonia-lyase gene (CcPAL1) and its involvement in the accumulation of caffeoyl quinic acids.
Biosynthesis of caffeoylquinic acids occurs via the phenylpropanoid pathway in which the phenylalanine ammonia-lyase (PAL) acts as a key-control enzyme. A full-length cDNA (pF6), corresponding to a PAL gene (CcPAL1), was isolated by screening a Coffea canephora fruit cDNA library and its corresponding genomic sequence was characterized. Amplification of total DNA from seven Coffea species revealed differences in intronic length. This interspecific polymorphism was used to locate the gene on a genetic map established for a backcross progeny between Coffea pseudozanguebariae and C. dewevrei. The CcPAL1 gene was found on the same linkage group, but genetically independent, as a caffeoyl-coenzyme A-O-methyltransferase gene, another gene intervening in the phenylpropanoid pathway. In the same backcross, a lower caffeoylquinic acid content was observed in seeds harvested from plants harbouring the C. pseudozanguebariae CcPAL1 allele. Involvement of the CcPAL1 allelic form in the differential accumulation of caffeoylquinic acids in coffee green beans is then discussed.
Mahesh
Venkataramaiah
V
IRD, Génomique et qualité du Café, UMR DGPC, BP 64501, 34304, Montpellier Cedex 5, France.
Rakotomalala
Jean Jacques
JJ
Le Gal
Lénaïg
L
Vigne
Hélène
H
de Kochko
Alexandre
A
Hamon
Serge
S
Noirot
Michel
M
Campa
Claudine
C
D000595:Amino Acid Sequence; D002109:Caffeic Acids / Q000378:metabolism*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D040503:Coffea / Q000201:enzymology* / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics* / Q000378:metabolism*; D011801:Quinic Acid / Q000378:metabolism*
D002109:Caffeic Acids; D018076:DNA, Complementary; D018744:DNA, Plant; D011801:Quinic Acid; D010650:Phenylalanine Ammonia-Lyase