An allele of gyrA prevents Salmonella enterica serovar Typhimurium from using succinate as a carbon source.
A mutant gyrA allele resulting in an A271E substitution in the DNA gyrase protein generated a strain unable to grow on the C(4)-dicarboxylates succinate, malate, and fumarate. Bacteria harboring gyrA751 displayed decreased negative supercoiling in cells. Expression of the dctA gene, which encodes the C(4)-dicarboxylate transporter, was reduced in a gyrA751 mutant, providing the first evidence that dctA expression is supercoiling sensitive and uncovering a simple metabolic screen for lesions in gyrase that reduce negative supercoiling.
Department of Bacteriology, University of Wisconsin-Madison, 420 Henry Mall, Rm. 120, Madison, WI 53706, USA.
George E
GE
Schmitz
Diana M
DM
Downs
D000483:Alleles; D019943:Amino Acid Substitution; D002244:Carbon / Q000378:metabolism*; D027081:DNA Gyrase / Q000235:genetics*; D004278:DNA, Superhelical / Q000235:genetics; D005650:Fumarates / Q000378:metabolism; D015870:Gene Expression; D008293:Malates / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics; D020125:Mutation, Missense; D012486:Salmonella typhimurium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism*
D004278:DNA, Superhelical; D005650:Fumarates; D008293:Malates; D026901:Membrane Transport Proteins; D002244:Carbon; C030298:malic acid; C032005:fumaric acid; D019802:Succinic Acid; D027081:DNA Gyrase