Expression of cytoskeletal proteins, cross-reacting with anti-CYP1A, in Mytilus sp. exposed to organic contaminants.
The possible use of cytoskeletal components as biomarkers of organic pollution in mussels has been investigated. Responses of non-muscular actin and tropomyosin (TM), two bivalve proteins that were recently demonstrated to cross-react with anti-fish-CYP1A, were analysed in digestive tissue of blue mussels (Mytilus sp.) exposed to a wide range of organic contaminants. The results were evaluated with ELISA and Western blot assays, utilising commercial monoclonal antibodies, and compared with expression of Hsp70, a marker of chemical stress. Furthermore, mussels were sampled from the Baltic Sea at sites with different degrees of pollution to assess the expression of these proteins, and to monitor seasonal changes in relation to energy reserves and water temperature. The results demonstrated that expression of microsomal actin was significantly higher (p<0.02) in mussels exposed to a brominated flame retardant (BDE-47), and lower, however not significantly, in specimens exposed to crude oil, alone and spiked with alkylphenols and PAHs. Hsp70 was strongly induced in all exposure groups, which also included bisphenol A and diallylphthalate. Furthermore, microsomal actin exhibited seasonal variations, and expression was negatively correlated with water temperature. No correlation was seen between actin and the microfilament-binding protein TM, indicating that regulation of these two cytoskeletal components are not coupled. Furthermore, parallel and significant (p<0.05) up-regulations of TM and Hsp70 were seen in individuals sampled from a strongly polluted field site, whereas the seasonal analysis showed that TM expression was positively correlated with energy reserves (total glycogen content) in mussels, suggesting the use of TM as a marker of growth. In conclusion, this study has demonstrated the cytoskeleton to be a target of contaminants in mussels, calling for further attention. Exposure-induced increase of microsomal actin can be interpreted either as stimulated actin synthesis, or re-arrangements of the dynamic microfilaments.
Jonsson
Henrik
H
Baltic Sea Research Institute, Rostock, Germany. jonsson@unipmn.it
Schiedek
Doris
D
Goksøyr
Anders
A
Grøsvik
Bjørn Einar
BE
D000199:Actins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D003429:Cross Reactions; D019363:Cytochrome P-450 CYP1A1 / Q000276:immunology*; D003598:Cytoskeletal Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D004781:Environmental Exposure; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015870:Gene Expression / Q000187:drug effects; D006003:Glycogen / Q000032:analysis; D018840:HSP70 Heat-Shock Proteins / Q000187:drug effects / Q000276:immunology; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D049878:Mytilus / Q000187:drug effects* / Q000378:metabolism; D009792:Oceans and Seas; D012044:Regression Analysis; D014335:Tropomyosin / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D000199:Actins; D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; D018840:HSP70 Heat-Shock Proteins; D014335:Tropomyosin; D014874:Water Pollutants, Chemical; D006003:Glycogen; D019363:Cytochrome P-450 CYP1A1