In vitro effects of some drugs on human red blood cell glucose-6-phosphate dehydrogenase enzyme activity.
In vitro effects of omeprazole, morphine sulphate, remifentanyl, ketamine and vankomycin were investigated on human red blood cell glucose-6 phosphate dehydrogenase (G-6PD) (E.C. 1.1.1.49) enzyme activity purified from human red blood cell by 2', 5'ADP-Sepharose 4B affinity gel. The obtained I50 values of omeprazole, morphine, remifentanil, ketamine and vankomycin were 3.24, 43.58, 97.6, 64.16 and 0.903 mM, respectively and the Ki constants for omeprazole, morphine and vankomycin were 8.22 +/- 2.055, 25.93 +/- 6.482, and 2.71 +/- 0.677 mM, respectively and they were non competitive inhibitors.
Department of Chemistry, Arts and Science Faculty, Ataturk University, Erzurum, Turkey. hozdemir@atauni.edu.tr
Hasan
H
Ozdemir
Mehmet
M
Ciftçi
D000701:Analgesics, Opioid / Q000494:pharmacology; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007649:Ketamine / Q000494:pharmacology; D009020:Morphine / Q000494:pharmacology; D009853:Omeprazole / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D000077208:Remifentanil
D000701:Analgesics, Opioid; D004791:Enzyme Inhibitors; D010880:Piperidines; D007649:Ketamine; D009020:Morphine; D005954:Glucosephosphate Dehydrogenase; D009853:Omeprazole; D000077208:Remifentanil