Modulation of polyene antibiotics self-association by ions from the Hofmeister series.
The toxicity of the antifungal polyene antibiotic amphotericin B (AMB) has been related to its low solubility, more specifically to a self-associated form termed toxic aggregate. In addition, AMB in aqueous medium gives rise to concentration, ionic strength, and time-dependent polydisperse systems. For this reason different approaches, including the use of several lipid aggregates, have been used in attempts to improve the drug's solubility and increase its therapeutic index. In this context, understanding AMB's self-association properties should help in the preparation of less toxic formulations. Ions from the Hofmeister series alter water properties: while kosmotropes (water structure makers-sulfate, citrate, phosphate) decrease solute solubility, chaotropes (water structure breakers-perchlorate, thiocyanate, trichloroacetate, and the neutral molecule urea) have opposite effects. This work reports a study of the effect of Hofmeister ions and urea on the self-aggregation of AMB and some of its derivatives. Optical absorption and circular dichroism spectra were used to monitor monomeric and aggregated antibiotic. While kosmotropes increased aggregation in a concentration-dependent manner, the opposite was observed for chaotropes. It is shown, for the first time, that thiocyanate and trichloroacetate can induce complete AMB monomerization. The understanding of these processes at the physicochemical and molecular levels and the possibility of modulating the aggregation state of AMB and its derivatives should contribute to elucidate the mechanisms of action and toxicity of this widely used antibiotic and to develop more efficient and less toxic preparations.
Department of Biochemistry, Institute of Chemistry, Universidade de São Paulo, CP 26077, CEP 05513-970 São Paulo, Brazil.
Mercedes
M
Toledo Grijalba
Monique
M
Chéron
Edward
E
Borowski
Jacques
J
Bolard
Shirley
S
Schreier
D000666:Amphotericin B / Q000737:chemistry; D000935:Antifungal Agents / Q000737:chemistry* / Q000633:toxicity; D002942:Circular Dichroism; D019343:Citric Acid; D007477:Ions / Q000737:chemistry; D009994:Osmolar Concentration; D010472:Perchlorates; D010710:Phosphates; D011090:Polyenes / Q000737:chemistry* / Q000633:toxicity; D012995:Solubility; D013057:Spectrum Analysis; D013431:Sulfates; D013861:Thiocyanates; D014238:Trichloroacetic Acid; D014508:Urea
D000935:Antifungal Agents; D007477:Ions; D010472:Perchlorates; D010710:Phosphates; D011090:Polyenes; D013431:Sulfates; D013861:Thiocyanates; D019343:Citric Acid; D014238:Trichloroacetic Acid; D000666:Amphotericin B; D014508:Urea; C031760:thiocyanate; C494474:perchlorate