New approach for high-throughput screening of drug activity on Plasmodium liver stages.
Plasmodium liver stages represent potential targets for antimalarial prophylactic drugs. Nevertheless, there is a lack of molecules active on these stages. We have now developed a new approach for the high-throughput screening of drug activity on Plasmodium liver stages in vitro, based on an infrared fluorescence scanning system. This method allowed us to count automatically and rapidly Plasmodium-infected hepatocytes, using different hepatic cells and different Plasmodium species, including Plasmodium falciparum. This new technique is well adapted for high-throughput drug screening and should facilitate the identification of new antimalarial compounds active on Plasmodium liver stages.
Gego
Audrey
A
Inserm U511, F-75013 Paris, France.
Silvie
Olivier
O
Franetich
Jean-François
JF
Farhati
Khemaïs
K
Hannoun
Laurent
L
Luty
Adrian J F
AJ
Sauerwein
Robert W
RW
Boucheix
Claude
C
Rubinstein
Eric
E
Mazier
Dominique
D
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002478:Cells, Cultured; D022781:Hepatocytes / Q000469:parasitology*; D006801:Humans; D010961:Plasmodium / Q000187:drug effects*
D000962:Antimalarials