[Tracking of the development of fecal bacterial community of diarrhea piglets by 16S rDNA techniques].
PCR and denaturing gradient gel electrophoresis (DGGE) were used to track the development of fecal bacterial community of 5 diarrhea piglets. Clone libraries were created from complete 16S rDNA of 42-day fecal sample of piglet 1, 2 and 5. From the library, clones had their V6-V8 regions matched predominant bands on the DGGE gel were then sequenced and their 16S rDNAs subjected to an online similarity search. Results revealed that DGGE profiles from fecal samples of 5 piglets changed form simple to complex, and then returned simple to complex, finally tended to be stable and diverse. DGGE profiles from day 2and 16 fecal samples of 5 piglets were simplest, with high similarity (84%). Their most predominant bands were E. coil. Their Shannon index were 1.38 +/- 0.55 and 1.97 +/- 0.26 respectively. DGGE profiles from day 10 of 5 piglets became quite complex, and had quite low similarity (10%) with DGGE profiles from day 2and 16 fecal samples of 5 piglets. The Shannon index was 2.12 +/- 0.24. E. coil still existed, but it was not the most predominant band. There were significant differences of predominant bands between DGGE profiles from day 27 fecal samples and those from day 35 and 42 fecal samples, with 26.28% and 39.2% of similarity respectively. The predominant bands from day 35 and 42 fecal samples of 5 piglets were tended to be stable, with 55% of similarity. The Shannon index of day 27, day 35 and 42 fecal bacterial communities were 2.19 +/- 0.29, 2.20 +/- 0.17 and 2.14 +/- 0.20 respectively. The fecal bacterial communities of day 42 health piglets were mainly consisted of species of Enterococcus, Streptococcus, Clostridium, Peptostreptococcus and Lactobacillus.
Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China. yaowen67jp@yahoo.com
Wen
W
Yao
Wei-yun
WY
Zhu
Sheng-Yong
SY
Mao
D000818:Animals; D001419:Bacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S