[The significance and effect of substance P on the expression of RANTES mRNA in allergic rhinitis].
OBJECTIVE
To study the significance and effect of SP on the expression of RANTES mRNA in allergic rhinitis (AR), and deeply understand the pathogenesis of AR.


METHODS
The model of AR was established in randomly selected healthy guinea pigs by using ovalbumin. Then the nasal dripping of SP was used to challenge the AR groups, as compared with the normal controls, in observation of the symptoms and histopathologic changes in the nasal mucosa. The number of eosinophil in the nasal lavage fluid was also counted. By means of RT-PCR, the expressions of RANTES mRNA in the nasal mucosa of the different groups were quantitatively compared.


RESULTS
SP challenge induced similar AR symptoms in the normal group and aggravated AR symptoms and inflammation in the nasal mucosa in the model of AR groups. The expression level of RANTES mRNA (P < 0.05) and the number of eosinophil (P < 0.01) in AR groups increased significantly after challenged with SP.


CONCLUSIONS
In the pathogenesis of AR, SP induces the expression of chemokine RANTES in nasal mucosa, promotes eosinophil chemotaxis and migration, induces and aggravates the allergic inflammatory reaction in AR.
Zhang
Ruxin
R
Department of Otolaryngology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. rxzhang@x263.net
Liu
Guojun
G
Wen
Wu
W
Yan
Zhiqiang
Z
Wu
Geping
G
D000818:Animals; D018946:Chemokine CCL5 / Q000187:drug effects / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D007249:Inflammation; D008297:Male; D009297:Nasal Mucosa / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology* / Q000473:pathology; D013373:Substance P / Q000494:pharmacology*
D018946:Chemokine CCL5; D012333:RNA, Messenger; D013373:Substance P