Structure of the manganese superoxide dismutase from Deinococcus radiodurans in two crystal forms.
The structure of the manganese superoxide dismutase (Mn-SOD; DR1279) from Deinococcus radiodurans has been determined in two different crystal forms. Both crystal forms are monoclinic with space group P2(1). Form I has unit-cell parameters a = 44.28, b = 83.21, c = 59.52 angstroms, beta = 110.18 degrees and contains a homodimer in the asymmetric unit, with structure refinement (R = 16.8%, R(free) = 23.6%) carried out using data to d(min) = 2.2 angstroms. Form II has unit-cell parameters a = 43.57, b = 87.10, c = 116.42 angstroms, beta = 92.1 degrees and an asymmetric unit containing two Mn-SOD homodimers; structure refinement was effected to a resolution of 2.0 angstroms (R = 17.2%, R(free) = 22.3%). The resulting structures are compared with that of Mn-SOD from Escherichia coli, with which they are shown to be essentially isostructural.
Macromolecular Crystallography Group, European Synchrotron Radiation Facility, 38043 Grenoble CEDEX 9, France.
Rebecca J
RJ
Dennis
Elena
E
Micossi
Joanne
J
McCarthy
Elin
E
Moe
Elspeth J
EJ
Gordon
Sigrid
S
Kozielski-Stuhrmann
Gordon A
GA
Leonard
Sean
S
McSweeney
D018360:Crystallography, X-Ray; D034301:Deinococcus / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology; D008958:Models, Molecular; D011487:Protein Conformation; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000302:isolation & purification
D013482:Superoxide Dismutase