Fast element mapping of titanium wear around implants of different surface structures.
The effect of unintended titanium release around oral implants remains a biological concern. The current study was undertaken to evaluate a new detection system of element mapping in biological probes. A new scanning electron microscopy-energy dispersive spectroscopy detection method was used to map the features of titanium contamination in peri-implant bone around implants with different surface structures. The amount of titanium wear was highest adjacent to titanium-plasma-sprayed surfaces, followed by sandblastered large grid acid-etched and smooth surfaces. A high sensitivity of titanium detection over large areas of bone tissue was observed. A high spatial resolution of titanium wear particles (20 nm) could be reached and correlated to the ultrastructural morphological features of peri-implant tissue. Cells adjacent to titanium wear revealed no signs of morphological alterations on a nanoscale level at early periods of implant/bone interaction. The new technique may serve as a fast and effective tool to evaluate titanium release effects in biological probes.
Meyer
Ulrich
U
Department of Maxillofacial and Plastic Facial Surgery, University of Düsseldorf, Düsseldorf, Germany. ulmeyer@uni-muenster.de
Bühner
Martin
M
Büchter
Andre
A
Kruse-Lösler
Birgit
B
Stamm
Thomas
T
Wiesmann
Hans Peter
HP
D000134:Acid Etching, Dental; D020906:Air Abrasion, Dental; D000539:Alveolar Process / Q000648:ultrastructure; D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D008297:Male; D008334:Mandible / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D015335:Molecular Probes; D049329:Nanostructures; D013499:Surface Properties; D013552:Swine; D013556:Swine, Miniature; D014025:Titanium / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D003764:Dental Materials; D015335:Molecular Probes; D014025:Titanium