Insight into the complex genetic network of tetraploid Atlantic salmon (Salmo salar L.): description of multiple novel Pax-7 splice variants.
Paired box transcription factor 7 (Pax-7) cDNA was isolated from the skeletal muscle and brain of alevin and adult stages of Atlantic salmon, identifying 10 variants categorised as novel or established insertions (ins) or deletions (del). Two putative Pax-7 paralogs were identified (denoted Pax-7alpha and Pax-7beta) on the basis of the length and sequences of intron 3 (218 and 248 bp) and versions of ins1 and ins2. Pax-7beta contained a threonine variant of ins1 (GQY[T]GPEYVYCGT), and a shortened variant of ins2 (GEAS). Pattern identification revealed the threonine variant of ins1 includes a potential phosphorylation site (casein kinase II). Thus, the tetraploid Atlantic salmon genome appears to contain at least two putative copies and multiple splice variants of Pax-7. In situ hybridisation localised Pax-7 to mononuclear cells in the fast muscle of adult Atlantic salmon, while quantitative real-time PCR showed Pax-7alpha to be more highly expressed in brain than in skeletal muscle.
Gatty Marine Laboratory, School of Biology, University of St Andrews, St Andrews, Fife KY16 8LB, UK.
Susan M
SM
Gotensparre
Eva
E
Andersson
Anna
A
Wargelius
Tom
T
Hansen
Ian A
IA
Johnston
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism / Q000502:physiology; D020033:Protein Isoforms / Q000737:chemistry*; D020317:Salmo salar / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D020033:Protein Isoforms; D014157:Transcription Factors