Anti-inflammatory mechanisms of the Tibetan herbal preparation Padma 28 in the vessel wall.
BACKGROUND
The Tibetan herbal preparation Padma 28 has been shown to act as an anti-atherosclerotic agent in advanced peripheral arterial occlusive disease. We tested the effect of aqueous Padma 28 extracts on both the Creactive protein (CRP) induced expression of the pro-inflammatory cell adhesion molecule E-selectin and the anti-atherosclerotic protective enzyme heme oxygenase- 1 (HO-1) in human aortic endothelial cells.


RESULTS
According to FACS analysis, quantitative RT-PCR and Western blot, CRP-induced E-selectin expression was completely prevented by aqueous Padma 28 extracts. Additionally, Padma 28 mediated an up to 60-fold upregulation of HO-1 mRNA as measured by quantitative RT-PCR. This upregulation could also be demonstrated on the protein level.


CONCLUSIONS
Aqueous extracts of the Tibetan herbal preparation Padma 28 inhibit CRP-induced expression of the inflammatory cell adhesion molecule E-selectin and lead to upregulation of the vascular protective enzyme HO-1 in human aortic endothelial cells. These properties may be responsible for its anti-atherosclerotic effects in peripheral arterial occlusive disease.
Exner
Markus
M
Klinisches Institut für Medizinische und Chemische Labordiagnostik, Institut für Medizinische Chemie, Medizinische Universität Wien, Austria. markus.exner@meduniwien.ac.at
Raith
Marianne
M
Holzer
Gregor
G
Gmeiner
Bernhard
B
Wagner
Oswald
O
Kapiotis
Stylianos
S
D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001161:Arteriosclerosis / Q000188:drug therapy*; D001808:Blood Vessels / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D002097:C-Reactive Protein / Q000032:analysis / Q000037:antagonists & inhibitors; D002478:Cells, Cultured; D019040:E-Selectin / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006801:Humans; D038861:Medicine, Tibetan Traditional / Q000379:methods*; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D016896:Treatment Outcome
D000893:Anti-Inflammatory Agents; D019040:E-Selectin; D010936:Plant Extracts; C066069:Padma 28; D002097:C-Reactive Protein; D051547:Heme Oxygenase-1