[Interrelation between thrombin structure and its stability].
Temperature inactivation of human thrombin has been studied when finding out the mechanism of this enzyme stabilization by amino acids. Effect of a number of amino acids on thrombin in the conditions (pH) of the highest activity of proteinase has been investigated. It is established that most amino acids are characterized to more or less extent by the protective action, when hampering the temperature inactivation of the enzyme. The correspondence was mainly found between the stabilizing effect of amino acids and thrombin specificity. Thrombin is stabilized by L-arginine and DL-lysine more intensively than by other amino acids. A stabilizing effect of L-glutamic acid was shown in contrast to the action of the latter on trypsin that was obviously connected with the original structure of the active centre of thrombin, that is the availability of anionic binding centre which includes Lys68, Arg72, Arg77. High thrombin stabilization by such amino acids as phenylalanine, DL-serine, DL-methonine was an exception. It was established that amino acids stabilize thrombin with formation of a compound with the reactive centre of its molecule, like the compounds enzyme-substrate. The macrostructure stability probably depends, to a considerable extent, on the state of the enzyme reactive centre: thrombin molecules, which contain a free reactive centre, are more labile than those which reactive centre is bound to the reagent of more or less specific character. The inhibition of the autolysis process may be another manifestation of thrombin stabilization by amino acids.
M V
MV
Kolodzeĭskaia
G L
GL
Volkov
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002384:Catalysis; D002417:Cattle; D003029:Coagulants / Q000737:chemistry*; D004355:Drug Stability; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D013379:Substrate Specificity; D013696:Temperature; D013917:Thrombin / Q000737:chemistry*; D013997:Time Factors
D000596:Amino Acids; D003029:Coagulants; D005340:Fibrinogen; D013917:Thrombin