G-protein complex mutants are hypersensitive to abscisic acid regulation of germination and postgermination development.
Abscisic acid (ABA) plays regulatory roles in a host of physiological processes throughout plant growth and development. Seed germination, early seedling development, stomatal guard cell functions, and acclimation to adverse environmental conditions are key processes regulated by ABA. Recent evidence suggests that signaling processes in both seeds and guard cells involve heterotrimeric G proteins. To assess new roles for the Arabidopsis (Arabidopsis thaliana) Galpha subunit (GPA1), the Gbeta subunit (AGB1), and the candidate G-protein-coupled receptor (GCR1) in ABA signaling during germination and early seedling development, we utilized knockout mutants lacking one or more of these components. Our data show that GPA1, AGB1, and GCR1 each negatively regulates ABA signaling in seed germination and early seedling development. Plants lacking AGB1 have greater ABA hypersensitivity than plants lacking GPA1, suggesting that AGB1 is the predominant regulator of ABA signaling and that GPA1 affects the efficacy of AGB1 execution. GCR1 acts upstream of GPA1 and AGB1 for ABA signaling pathways during germination and early seedling development: gcr1 gpa1 double mutants exhibit a gpa1 phenotype and agb1 gcr1 and agb1 gcr1 gpa1 mutants exhibit an agb1 phenotype. Contrary to the scenario in guard cells, where GCR1 and GPA1 have opposite effects on ABA signaling during stomatal opening, GCR1 acts in concert with GPA1 and AGB1 in ABA signaling during germination and early seedling development. Thus, cell- and tissue-specific functional interaction in response to a given signal such as ABA may determine the distinct pathways regulated by the individual members of the G-protein complex.
Pandey
Sona
S
Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802-5301, USA.
Chen
Jin-Gui
JG
Jones
Alan M
AM
Assmann
Sarah M
SM
D000040:Abscisic Acid / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics / Q000502:physiology*; D044387:GTP-Binding Protein beta Subunits / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000187:drug effects / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000502:physiology*; D012639:Seeds / Q000254:growth & development / Q000378:metabolism; D015398:Signal Transduction
C089590:AGB1 protein, Arabidopsis; D029681:Arabidopsis Proteins; C112029:GCR1 protein, Arabidopsis; C429765:GPA1 protein, Arabidopsis; D044385:GTP-Binding Protein alpha Subunits; D044387:GTP-Binding Protein beta Subunits; D043562:Receptors, G-Protein-Coupled; D000040:Abscisic Acid; D005947:Glucose