Micellization and reversible pH-sensitive phase transfer of the hyperbranched multiarm PEI-PBLG Copolymer.
A novel, hyperbranched, amphiphilic multiarm biodegradable polyethylenimine-poly(gamma-benzyl-L-glutamate) (PEI-PBLG) copolymer was prepared by the ring-opening polymerization of gamma-benzyl-L-glutamate-N-carboxyanhydride (BLG-NCA) with hyperbranched PEI as a macroinitiator. The copolymer could self-assemble into core-shell micelles in aqueous solution with highly hydrophobic micelle cores. As the PBLG content was increased, the size of the micelles increased and the critical micelle concentration (CMC) decreased. The surface of the micelles had a positive zeta potential. The cationic micelles were capable of complexing with plasmid DNA (pDNA), which could be released subsequently by treatment with polyanions. The PEI-PBLG copolymer formed unimolecular micelles in chloroform solution. The pH-sensitive phase-transfer behavior exhibited two critical pH points for triggering the encapsulation and release of guest molecules. Both the encapsulation and release processes were rapid and reversible. Under strong acidic or alkaline conditions, the release process became partially or completely irreversible. Thus, this copolymer system should be an attractive candidate for a gene- or drug-delivery system in aqueous media and could provide the phase-transfer carriers between water and organic media.
Tian
Huayu
H
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, China.
Chen
Xuesi
X
Lin
Hao
H
Deng
Chao
C
Zhang
Peibiao
P
Wei
Yen
Y
Jing
Xiabin
X
D001673:Biodegradation, Environmental; D002725:Chloroform / Q000737:chemistry; D016503:Drug Delivery Systems; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D018625:Microscopy, Atomic Force; D044367:Phase Transition; D011094:Polyethyleneimine / Q000031:analogs & derivatives* / Q000737:chemistry; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry
D008823:Micelles; C521445:polyethylenimine-poly(gamma-benzylglutamate); D011099:Polyglutamic Acid; D002725:Chloroform; D011094:Polyethyleneimine