[Expression of perlecan in laryngeal carcinoma cell and its significance].
OBJECTIVE
To study the expression of perlecan in human laryngeal carcinoma cells and its significance.


METHODS
The expression of perlecan mRNA in human laryngeal carcinoma cells, Hep-2 cells were investigated by using the techniques of semi-quantify RT-PCR. The expression of perlecan in the Hep-2 cells was investigated by using the techniques of immunohistochemistry.


RESULTS
It showed that the expression level of perlecan and perlecan mRNA significantly increased in Hep-2 cells as compared with the normal cells, Hacat cells (P < 0.01).


CONCLUSIONS
These data raise the possibility that perlecan many play key roles in the growth,invasion and metastasis of human laryngeal carcinoma cells through either a paracrine or an autocrine mechanism.
Department of Otolaryngology, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China.
Guangli
G
Chen
Juan
J
Ding
Linghui
L
Luo
Yingpeng
Y
Liu
Kaisheng
K
Yan
Pei
P
Chen
Peng
P
Song
Yong
Y
Fu
Jianting
J
Wang
Shusheng
S
Gong
D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics
D019812:Heparan Sulfate Proteoglycans; D012333:RNA, Messenger; C071927:perlecan