Bowline, a novel protein localized to the presomitic mesoderm, interacts with Groucho/TLE in Xenopus.
Cells in the prospective somite of Xenopus laevis embryos rotate in an orchestrated manner to form a segregated somite. The prospective somite boundaries are prepatterned by gene expressions in the unsegmented presomitic mesoderm (PSM). However, the roles of polarized gene expression in this boundary formation are not well elucidated. Here we identified a novel gene, bowline, which localizes to the anterior halves of S-II, III in the PSM of X. laevis. Bowline associated with corepressor XGrg-4, a Xenopus homolog of Groucho/TLE protein. A WRPW tetrapeptide motif in Bowline was prerequisite for coprecipitation with XGrg-4 and for downregulation of X-Delta-2 by bowline RNA injection. This study indicates that Bowline is a novel protein interacting with Groucho/TLE and may play a role in somitogenesis in X. laevis.
Kondow
Akiko
A
Department of Life Science Biology, Graduate School of Arts and Science, The University of Tokyo, Japan.
Hitachi
Keisuke
K
Ikegame
Tempei
T
Asashima
Makoto
M
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019521:Body Patterning; D056970:Co-Repressor Proteins; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D019170:Somites / Q000166:cytology / Q000378:metabolism; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*
D056970:Co-Repressor Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D051880:Receptors, Notch; D012097:Repressor Proteins; C523700:TLE4 protein, Xenopus; D029867:Xenopus Proteins; C512374:ripply2.2 protein, Xenopus