Poly(ether imide) membranes modified with poly(ethylene imine) as potential carriers for epidermal substitutes.
Poly(ether imide) (PEI) membranes were modified with a linear low-molecular weight (PETIM_0.6) and a branched high-molecular weight poly(ethylene imine) (PETIM_60). The membrane surfaces became more hydrophilic and the zeta potentials were shifted from negative to positive zeta values after immobilisation of both PETIM. These measurements also indicated the presence of a swollen surface layer in the case of PETIM_60, while a regular structuring of the surface was observed with scanning force microscopy for PETIM_0.6. A human keratinocyte cell line HaCaT was cultured on the different membranes. It was found that HaCaT cell growth was stimulated by PETIM_0.6. Cells reached earlier confluence on this substratum, while their growth was inhibited on a PEI membrane modified with PETIM_60, which makes PEI membranes modified with PETIM_0.6 a promising material for in vitro culture of epidermal transplants.
GKSS Research Centre, Institute of Polymer Research, Kantstrasse 55, D-14513 Teltow, Germany.
Christiane
C
Trimpert
Gregor
G
Boese
Wolfgang
W
Albrecht
Klaus
K
Richau
Thomas
T
Weigel
Andreas
A
Lendlein
Thomas
T
Groth
D000328:Adult; D002455:Cell Division; D002460:Cell Line; D002463:Cell Membrane Permeability; D049109:Cell Proliferation; D000078404:Epidermal Cells; D006801:Humans; D007097:Imines / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D011095:Polyethylenes / Q000737:chemistry*; D017768:Skin, Artificial
D007097:Imines; D008567:Membranes, Artificial; D011095:Polyethylenes; C505405:poly(ethylene imine)