Genotypic characterization and phylogenetic relations of Pseudomonas sp. (Formerly P. stutzeri) OX1.
Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment length polymorphism (MERFLP) analysis suggested that Pseudomonas sp. OX1 could not be classified as P. stutzeri. Phylogenetic analyses based on 16S rRNA and gyrB genes further confirmed that this strain belongs to the Pseudomonas (sensu stricto) genus, but not to the stutzeri species. The data obtained demonstrated that Pseudomonas sp. OX1 belongs to intrageneric cluster II and is related to the P. fluorescens-P. syringae complex.
Dipartimento di Biologia Strutturale e Funzionale, Università dell'Insubria, via Dunant 3, Varese, 21100, Italy.
Francesca
F
Radice
Viviana
V
Orlandi
Valentina
V
Massa
Lucia
L
Cavalca
Antonella
A
Demarta
Thomas K
TK
Wood
Paola
P
Barbieri
D015373:Bacterial Typing Techniques; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics*; D044223:Pseudomonas stutzeri / Q000145:classification / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase