[Carboanhydrase activity in halophilic anaerobes from soda lakes].
The activity and cellular localization of carboanhydrase (CA) in two alkaliphilic anaerobes growing in soda lakes at pH 9-10 was studied. CA activity in the cell extracts of the acetogenic bacterium Natroniella acetigena was comparable to that of the neutrophilic acetogens. Hydrogenotrophically grown cells of Desulfonatronum lacustre exhibited higher CA activity compared to the cells grown on media with formate. High CA activity in the cytoplasmic fraction and the absence of high activity in the extracellular fraction were demonstrated. We propose that the cytoplasmic CA in alkaliphilic sulfate-reducers participates in conversion of bicarbonate to CO2, which is reduced in the cell to acetate via the acetyl-CoA pathway.
A V
AV
Pitriuk
M A
MA
Pusheva
E V
EV
Kupriianova
D000105:Acetyl Coenzyme A / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001639:Bicarbonates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000378:metabolism*; D003013:Clostridium / Q000201:enzymology* / Q000254:growth & development; D003593:Cytoplasm / Q000201:enzymology*; D003901:Desulfovibrio / Q000201:enzymology* / Q000254:growth & development; D014871:Water Microbiology
D001426:Bacterial Proteins; D001639:Bicarbonates; D002245:Carbon Dioxide; D000105:Acetyl Coenzyme A; D002256:Carbonic Anhydrases