Gene expression profiles of the aurora family kinases.
The evolutionarily conserved Aurora family kinases, a family of mitotic serine/threonine kinases, has three members in humans (Aurora-A, -B and -C). Overexpression of Aurora family members, particularly Aurora-A, has been reported in many human cancers and cell lines. In this study, we present evidence based on comparative gene expression analysis via quantitative RT-PCR to delineate the relative contributions of these kinases in 60 cell lines and statistical analysis in five different human cancer microarray datasets. The analysis demonstrated the selective upregulation of these Aurora members in various cancers. In general, Aurora-A exhibited the highest expression levels, with substantially decreased quantities of the Aurora-C transcript detected relative to Aurora-A and -B. Moreover, to characterize the roles of each Aurora member, which share many similarities, we investigated the expression profiles of the family in normal tissues and a panel of different phases of the HeLa cell cycle. Finally, both Aurora-A and -B were overexpressed in a majority of esophageal tumor tissues in comparison to the normal variants. Taken together, the results show that each Aurora member exhibits distinct expression patterns, implying that they are engaged in different biological processes to accomplish more elaborate cell physiological functions in higher organisms.
Lin
Yong-Shiang
YS
Division of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli County 350, Taiwan, ROC.
Su
Li-Jen
LJ
Yu
Chang-Tze Ricky
CT
Wong
Fen-Hwa
FH
Yeh
Hsu-Hua
HH
Chen
Su-Liang
SL
Wu
Jiunn-Chyi
JC
Lin
Wey-Jin
WJ
Shiue
Yow-Ling
YL
Liu
Hsiao-Sheng
HS
Hsu
Shih-Lan
SL
Lai
Jin-Mei
JM
Huang
Chi-Ying F
CY
D064108:Aurora Kinase C; D064127:Aurora Kinases; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured / Q000378:metabolism
D012333:RNA, Messenger; D012334:RNA, Neoplasm; C576927:AURKC protein, human; D064108:Aurora Kinase C; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases