[Study on the treatment of nasal polyposis with bleomycin A5 local injection].
OBJECTIVE
To study the treatment mechanism of nasal polyposis by locally injecting Bleomycin A5.


METHODS
The specimens from nineteen cases with nasal polyposis obtained before and after treatment with Bleomycin A5 were studied with immunohistochemical technique and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL).


RESULTS
The apoptosis index (AI) of eosinophil was (20.53 +/- 7.66) % before treatment and (44.47 +/- 8.97) % after treatment. There was significantly difference between them (P < 0.01). The rate of TGF-beta1 positive expression in eosinophil after treatment (74.74 +/- 5.22) % was significantly lower than that before treatment (85.18 +/- 8.07) % (P < 0.05). The expression of TGF-beta1 in matrix was also decreased (P < 0.05).


CONCLUSIONS
Bleomycin A5 could cure nasal polyposis by promoting eosinophil apoptosis and reducing the expression of TGF-beta1.
Department of Otorhinolaryngology, Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China. myu@fmmu.edu.cn
Xianghong
X
Zhang
Jidong
J
Zou
Bin
B
Sun
Jianmin
J
Liang
Lin
L
Zhao
Baoshu
B
Li
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001761:Bleomycin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D053773:Transforming Growth Factor beta1; D001761:Bleomycin; C025703:bleomycetin