The use of radiotelemetry to evaluate electrographic seizures in rats with kainate-induced epilepsy.
Temporal lobe epilepsy in humans is a chronic condition with a highly variable temporal evolution. Animal models of this disorder have been developed to recapitulate many of the characteristics seen in humans with temporal lobe epilepsy. These animal models generate chronic spontaneous electrographic and motor seizures with a progressive increase in frequency over many months. In order to understand the underlying cellular and molecular mechanisms driving epileptogenesis, a practical means for accurately assessing seizure progression over this extended time period must be devised. In this report, we describe the use of a three-channel radiotelemetry system to record spontaneous electrographic interictal "spikes" and seizure activity from the cortical surface and the two hippocampi. This approach has allowed continuous recording before, during, and several months after kainate-induced status epilepticus. The important advantages of this approach are the potential for long-term continuous electrographic recording with comparatively unrestricted behavior; the disadvantages include increased cost, surgical difficulty and lower frequency-response in the recordings.
Department of Biomedical Sciences, Anatomy and Neurobiology Section, Colorado State University, Fort Collins, CO 80523, USA.
Philip
P
Williams
Andrew
A
White
Damien
D
Ferraro
Suzanne
S
Clark
Kevin
K
Staley
F Edward
FE
Dudek
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001921:Brain / Q000187:drug effects / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000503:physiopathology; D003292:Convulsants / Q000494:pharmacology; D003362:Cost-Benefit Analysis; D004195:Disease Models, Animal; D004569:Electroencephalography / Q000295:instrumentation / Q000379:methods*; D004582:Electronics, Medical / Q000295:instrumentation / Q000379:methods; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods*; D004827:Epilepsy / Q000139:chemically induced / Q000175:diagnosis / Q000503:physiopathology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000503:physiopathology; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D008839:Microelectrodes / Q000592:standards; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009482:Neurophysiology / Q000295:instrumentation / Q000379:methods*; D011237:Predictive Value of Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D013226:Status Epilepticus / Q000139:chemically induced / Q000175:diagnosis / Q000503:physiopathology; D013686:Telemetry / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
D003292:Convulsants; D018690:Excitatory Amino Acid Agonists; D007608:Kainic Acid