Sucralfate protects intestinal epithelial cells from radiation-induced apoptosis in rats.
Radiotherapy for malignant pelvic disease is often followed by acute radiation colitis (ARC). It has been reported that sucralfate treatment has a protective effect against ARC, though the mechanisms of action are unknown. The effects of sucralfate on X-ray radiation-induced apoptosis was studied at 4 Gy in the colonic crypt cells of rats. Sucralfate enemas given prior to radiation resulted in the following: (1) reduction in number of apoptotic colonic crypt cells; (2) reduction in number of caspase-3 positive cells; (3) decreases in p53 accumulation and p21 expression; (4) decreases of Bax/Bcl-2 ratio. The protective effects of sucralfate against ARC may be partially due to the suppression of radiation-induced apoptosis by way of p53 in the colon and the protection of the colonic epithelial stem cell region.
Matsuu-Matsuyama
Mutsumi
M
Department of Molecular Pathology, Nagasaki University Graduate School of Biomedical Sciences, Japan. f1173@cc.nagasaki-u.ac.jp
Shichijo
Kazuko
K
Okaichi
Kumio
K
Ishii
Kouji
K
Wen
Chung Yang
CY
Fukuda
Eiichiro
E
Nakayama
Toshiyuki
T
Nakashima
Masahiro
M
Okumura
Yutaka
Y
Sekine
Ichiro
I
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D011832:Radiation Injuries / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D013392:Sucralfate / Q000008:administration & dosage*; D016896:Treatment Outcome; D014916:Whole-Body Irradiation
D011837:Radiation-Protective Agents; D013392:Sucralfate