A short 5' flanking region containing conserved sequences is required for silkworm alanine tRNA gene activity.
Using partially deleted genes, we have identified an upstream control signal required for transcription of a Bombyx mori (silkworm) tRNA(2) (Ala) gene. The 5' boundary of this essential region lies between 34 and 11 nucleotides preceding the transcription initiation site. Sequences in the 5' half of the tRNA(2) (Ala) coding region are also important. Both regions contain oligonucleotides that are conserved among several Bombyx genes transcribed by RNA polymerase III, suggesting that these sequences may have a general control function.
Institute of Molecular Biology and Department of Biology, University of Oregon, Eugene, Oregon 97403.
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Larson
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Bradford-Wilcox
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Young
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Sprague