[Degradation of proteinaceous substrates by xylotrophic basidiomycetes].
The ability of various xylotrophs to produce extracellular proteolytic enzymes has been studied, with emphasis on medium-related factors regulating their secretion. Direct measurement of proteolytic activity in the culture liquid and postelectrophoresis determination of protease activity in polyacrylamide gel copolymerized with gelatin demonstrated that the secreted enzymes are quantitatively and qualitatively diverse. Activity levels of extracellular proteolytic enzymes strongly depend on pH and contents of protein and carbohydrate in the medium. All secreted proteases notably differed in molecular weight (of 51 kDa or higher and in excess of 95 kDa) and belonged mostly to two classes of proteolytic enzymes (serine proteases and metalloproteinases).
Ia E
IaE
Dunaevskiĭ
Dong
D
Zhang
A R
AR
Matveeva
G A
GA
Beliakova
M A
MA
Belozerskiĭ
D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media; D005656:Fungal Proteins / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D045726:Metalloproteases / Q000378:metabolism*; D008970:Molecular Weight; D012697:Serine Endopeptidases / Q000378:metabolism*; D013379:Substrate Specificity
D003470:Culture Media; D005656:Fungal Proteins; D045726:Metalloproteases; D012697:Serine Endopeptidases