[Isolation and purification of an inhibitor on platelet aggregation from Ixodes sinensis].
OBJECTIVE
To study the bioactive components in ticks which inhibit platelet aggregation, and to understand the molecular mechanism of tick-host interaction.


METHODS
Sephadex G-50 gel filtration and high performance liquid chromatography (HPLC) were used to purify the platelet aggregation inhibitor from lxodes sinensis. Its molecular weight and purity were checked by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Platelet-rich plasma (PRP) of rabbit was used to examine the function of platelet aggregation inhibitor.


RESULTS
A purified platelet aggregation inhibitor was identified from L. sinensis with a molecular weight of 8 065. It inhibited platelet aggregation induced by ADP with strong potency. The inhibition of platelet aggregation reached over 90% under a concentration of 10 microg/ml.


CONCLUSIONS
An inhibitor of platelet aggregation from L. sinensis was identified, which may play an important role for ticks to successfully get blood meal from their hosts.
Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
Yong-hou
YH
Liu
Chun-hua
CH
Xu
Zhi-gang
ZG
Liu
Jian-guo
JG
Liang
Ren
R
Lai
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D026863:Ixodidae / Q000737:chemistry*; D008970:Molecular Weight; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010975:Platelet Aggregation Inhibitors