Cowpea mosaic virus for material fabrication: addressable carboxylate groups on a programmable nanoscaffold.
For the first time, decoration of surface-exposed carboxylate groups on Cowpea mosaic virus particles is reported, thus increasing the number and types of addressable surface groups on this nanoscaffold. First, the addressabilty of carboxylates was demonstrated using a carboxylate-selective fluorescent dye, N-cyclohexyl-N'-(4-(dimethylamino)naphthyl)carbodiimide. Second, it was shown that the virions can be decorated with approximately 180 redox active, methyl(aminopropyl)viologen moieties by coupling to the surface carboxylates. The display of multiple redox centers on the virus particle surface may lead to the development of novel electron-transfer mediators in redox catalysis, to biosensors, and to nanoelectronic devices such as molecular batteries.
Department of Biological Chemistry, John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom.
Nicole F
NF
Steinmetz
George P
GP
Lomonossoff
David J
DJ
Evans
D001698:Biomedical Engineering; D015374:Biosensing Techniques; D002264:Carboxylic Acids / Q000737:chemistry*; D002384:Catalysis; D017798:Comovirus / Q000378:metabolism*; D004581:Electronics; D004583:Electrons; D004996:Ethidium / Q000737:chemistry; D005456:Fluorescent Dyes / Q000494:pharmacology; D008956:Models, Chemical; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D010084:Oxidation-Reduction; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties
D002264:Carboxylic Acids; D005456:Fluorescent Dyes; D012997:Solvents; D004996:Ethidium