[Identification strategy of RHCE gene variants at the National Blood Group Reference Laboratory: impact on transfusion safety].
The RH blood group is the most polymorphic and immunogenic blood group system. The RH locus is composed of 2 highly homologous genes: the RHD gene encoding the D polypeptide; and the RHCE gene, encoding C or c together with either E or e polypeptides. Numerous variants exist for both RHD and RHCE genes. Among them we were interested in the serological and molecular definition of numerous pre-published RHCE variants encountered in different populations. The identification of these variants is crucial to ensure the transfusion safety for patients expressing these variants. Here we propose a procedure to identify some of them and discuss the adequate transfusion strategy. This procedure has enable us to identify new variants to be presented at the symposium.
Ansart-Pirenne
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Centre national de référence pour les groupes sanguins (CNRGS), institut national de la transfusion sanguine, 20, rue Bouvier, BP 79, 75522 Paris cedex 11, France. hansart@ints.fr
D001787:Blood Group Incompatibility / Q000517:prevention & control; D001788:Blood Grouping and Crossmatching / Q000379:methods* / Q000592:standards; D005006:Ethnicity / Q000235:genetics; D005602:France; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007518:Isoantibodies / Q000032:analysis / Q000097:blood; D008958:Models, Molecular; D012204:Rh-Hr Blood-Group System / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000276:immunology; D018029:Rho(D) Immune Globulin; D065227:Transfusion Reaction
D007518:Isoantibodies; C066468:RHCE protein, human; C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin; C026410:Rho(D) antigen