[Studies on chemical constituents of hairy root of Cassia obtusifolia].
OBJECTIVE
To study the chemical constituents in hairy roots of Cassia obtusifolia.


METHODS
The hairy roots of C. obtusifolia were induced with Agrobacterium rhizogenes LBA9402 from cotyledons and cultured in MSO liquid medium. The compounds were isolated by silica gel, polyamide and Sephadex LH-20 column chromatography, and the structures were elucidated by employing chemical and spectral methods


RESULTS
Eight compounds were isolated from the ethyl acetate fraction of 95% EtOH extract of the transformed roots of C. obtusifolia. They are betulinic acid, chrysophanol, physcion, stigmasterol, 1-hydroxy-7-methoxy-3-methyl-anthraquinone, 8-O-methylchrysophanol, 1-O-methylchrysophanol and aloe-emodin, and aloe-emodin was isolated from the hairy roots of C. obtusifolia for the first time.


CONCLUSIONS
The hairy roots of C. obtusifolia have the ability to synthesize the similar chemical constituents as the original plants.
College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China.
Shi-Hai
SH
Yang
Hong-Zhu
HZ
Guo
De-An
DA
Guo
Jun-Hua
JH
Zheng
D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification; D002366:Cassia / Q000737:chemistry* / Q000254:growth & development / Q000382:microbiology; D018548:Cotyledon / Q000254:growth & development / Q000382:microbiology; D003470:Culture Media; D004642:Emodin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*; D053978:Pentacyclic Triterpenes; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development / Q000382:microbiology; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development / Q000382:microbiology; D012231:Rhizobium; D046509:Tissue Culture Techniques / Q000379:methods; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification; D000094062:Betulinic Acid
D000880:Anthraquinones; D003470:Culture Media; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; C518327:aloe emodin; C008905:physcione; D004642:Emodin; C027113:chrysophanic acid; D000094062:Betulinic Acid