Composite magnetic particles as carriers for laccase from Trametes versicolor.
In this paper we report a study of laccase immobilisation on different kinds of carrier particles. The immobilisation of enzyme on the particle surface with respect to the immobilisation efficiency and the properties of the immobilised enzymes is discussed. The immobilisation of laccase on polystyrene particles bearing reactive beta-diketone groups is characterised by high efficiency, but grafting of the enzyme increases the stability of the colloidal system, which makes the separation/purification procedure difficult. Additionally, the extreme colloidal stability of the immobilisates hinders the application of such particles with immobilised enzymes in some applications where the recycling of the enzyme should be performed. It has been found that hybrid PS-AAEM particles equipped with maghemite show similar immobilisation efficiency to that of their analogues without maghemite and can additionally be manipulated in magnetic fields. The activity of the immobilised laccase is much higher in the pH region 5-7 and the temperature range 50-70 degrees C as compared with that of the free enzyme. Immobilised enzymes also exhibit much better storage stability.
Institut für Makromolekulare Chemie und Textilchemie, Technische Universität Dresden, 01062 Dresden, Germany. Andrij.Pich@chemie.tu-dresden.de
Andrij
A
Pich
Sanchita
S
Bhattacharya
Hans-Juergen P
HJ
Adler
Tobias
T
Wage
Anna
A
Taubenberger
Zheng
Z
Li
Karl-Heinz
KH
van Pee
Ulrike
U
Böhmer
Thomas
T
Bley
D001487:Basidiomycota / Q000201:enzymology*; D004795:Enzyme Stability; D042845:Laccase / Q000737:chemistry*; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D013056:Spectrophotometry, Ultraviolet
D042845:Laccase