Mannose selection system used for cucumber transformation.
The selectable marker system, which utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate, was adapted for Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.). Only transformed cells were capable of utilizing mannose as a carbon source. The highest transformation frequency of 23% was obtained with 10 g/l mannose and 10 g/l sucrose in the medium. Molecular, genetic analysis, and PMI activity assay showed that the regenerated shoots contained the pmi gene and the gene was transmitted to the progeny in a Mendelian fashion. The results indicated that the mannose selection system, which is devoid of the disadvantages of antibiotic or herbicide selection, could be used for cucumber Agrobacterium-mediated transformation.
He
Zhengquan
Z
Biotechnology Research Center, China Three Gorges University, Yichang 443002, PR China.
Duan
Zhenzhen
Z
Liang
Wei
W
Chen
Faju
F
Yao
Wei
W
Liang
Hongwei
H
Yue
Chaoyin
C
Sun
Zongxiu
Z
Chen
Fan
F
Dai
Jianwu
J
D018553:Cucumis sativus / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors; D018525:Germination; D008358:Mannose / Q000378:metabolism* / Q000494:pharmacology*; D010941:Plant Tumors; D030821:Plants, Genetically Modified; D012639:Seeds / Q000502:physiology; D014170:Transformation, Genetic
D008358:Mannose