[Simultaneous determination of resveratrol, emodin, chrysophanol, physcion, in root of Polygonam cuspidatum and its extract by HPLC].
OBJECTIVE
To establish a HPLC method for simultaneous determination of resveratrol, emodin, chrysophanol, physcion in root of Polygonum cuspidatum and its extract.


METHODS
The separation was performed in a Hypersil ODS C18 column with a mobile phase of Acetonitrile-1%HAc. The flow rate was 1.0 mL x min(-1) and detection wavelength was at 287 nm.


RESULTS
The mean recovery and RSD of resveratrol, emodin, chrysophanol, physcion was 101.8%, RSD 1.7%; 96.2%, RSD 1.4%; 99.7%, RSD 1.0%; 98.60%, RSD 0.97% respectively.


CONCLUSIONS
The method is accurate, simple and reliable. It can be used for quality control of P. cuspidatum and its extract.
Hunan Normal University, Changsha 410081, China.
Hong-Mei
HM
Yang
Bo
B
Chen
Jian-Guo
JG
Zeng
Jian-Rong
JR
Miao
D000880:Anthraquinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis*; D045468:Fallopia japonica / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis*
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D013267:Stilbenes; C008905:physcione; D004642:Emodin; C027113:chrysophanic acid; D000077185:Resveratrol