Nucleic acid-functionalized Pt nanoparticles: Catalytic labels for the amplified electrochemical detection of biomolecules.
Nucleic acid-functionalized Pt nanoparticles (Pt-NPs) act as catalytic labels for the amplified electrochemical detection of DNA hybridization and aptamer/protein recognition. Hybridization of the nucleic acid-modified Pt-NPs with a sensing nucleic acid/analyte DNA complex associated with an electrode enables the amperometric, amplified, detection of the DNA by the Pt NP electrocatalyzed reduction of H2O2 (sensitivity limit, 1 x 10(-11) M). Similarly, the association of aptamer-functionalized Pt- NPs to a thrombin aptamer/thrombin complex associated with an electrode allowed the amplified, electrocatalytic detection of thrombin with a sensitivity limit corresponding to 1 x 10(-9) M.
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Ronen
R
Polsky
Ron
R
Gill
Lubov
L
Kaganovsky
Itamar
I
Willner
D052157:Aptamers, Nucleotide / Q000032:analysis; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D021881:DNA, Catalytic / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D052156:SELEX Aptamer Technique / Q000379:methods; D012680:Sensitivity and Specificity; D013917:Thrombin / Q000032:analysis
D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic; D011506:Proteins; C081676:thrombin aptamer; D010984:Platinum; D006046:Gold; D004247:DNA; D006861:Hydrogen Peroxide; D013917:Thrombin