In vitro fermentation of mixed linkage glucooligosaccharides produced by Gluconobacter oxydans NCIMB 4943 by the human colonic microflora.
The aim of this study was to develop selectively fermented (prebiotic) carbohydrate molecules which would also result in the generation of butyric acid. Gluco-oligosaccharides produced by Gluconobacter oxydans NCIMB 4943 from various types of maltodextrins were evaluated for their fermentation by mixed cultures of human colonic microflora. The selectivity of growth of desirable bacteria (bifidobacteria, lactobacilli) was studied in stirred pH-controlled (6.8) batch cultures. Bacterial populations were enumerated using fluorescent in situ hybridization (FISH). Gluco-oligosaccharides resulted in significantly (P<0.05) increased numbers of bifidobacteria and lactobacilli within 24 hours. Bacteroides, clostridial and eubacterial populations were slightly decreased at 48 h. There was very little difference in selectivity between the maltodextrin substrates and the products, although maltodextrin displayed a slightly less selective fermentation than the gluco-oligosaccharide products, also stimulating the growth of bacteroides, clostridia and eubacteria. Gluco-oligosaccharides, produced from G19 maltodextrin, resulted in the best prebiotic effect with the highest prebiotic index (PI) of 5.90 at 48 hours. Acetate, propionate and butyrate were all produced from gluco-oligosaccharides, derived from G19 maltodextrin, at 48 hours but no lactate or formate were detected.
Wichienchot
S
S
Department of Industrial Biotechnology, Prince of Songkla University, Hatyai, Thailand.
Prasertsan
P
P
Hongpattarakere
T
T
Gibson
G R
GR
Rastall
R A
RA
D019342:Acetic Acid / Q000378:metabolism; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D001439:Bacteroides / Q000254:growth & development; D001644:Bifidobacterium / Q000254:growth & development / Q000378:metabolism; D002087:Butyrates / Q000378:metabolism; D003013:Clostridium / Q000254:growth & development; D003106:Colon / Q000382:microbiology*; D015169:Colony Count, Microbial / Q000379:methods; D005285:Fermentation; D005561:Formates / Q000378:metabolism; D020600:Gluconobacter oxydans / Q000378:metabolism*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000254:growth & development / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism; D013997:Time Factors
D002087:Butyrates; D005561:Formates; D009844:Oligosaccharides; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D011422:Propionates; C030544:formic acid; D019344:Lactic Acid; C008315:maltodextrin; C029658:propionic acid; D019342:Acetic Acid