Calcium signaling and sugar-induced activation of plasma membrane H(+)-ATPase in Saccharomyces cerevisiae cells.
In this work, we show that glucose-induced activation of plasma membrane H(+)-ATPase from Saccharomyces cerevisiae is strongly dependent on calcium metabolism and that the glucose sensor Snf3p works in a parallel way with the G protein Gpa2p in the control of the pathway. The role of Snf3p is played by the Snf3p C-terminal tail, since in a strain with the deletion of the SNF3 gene, but also expressing a chimera protein formed by Hxt1p (a glucose transporter) and the Snf3p C-terminal tail, a normal glucose-activation process can be observed. We present evidences indicating that Snf3p would be the sensor for the internal signal (phosphorylated sugars) of this pathway that would connect calcium signaling and activation of the plasma membrane ATPase. We also show that Snf3p could be involved in the control of Pmc1p activity that would regulate the calcium availability in the cytosol.
Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Departamento de Farmácia, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro, 35.400-000 Ouro Preto, MG, Brazil.
Maria José M
MJ
Trópia
Anamaria S
AS
Cardoso
Renata
R
Tisi
Luciano G
LG
Fietto
Juliana L R
JL
Fietto
Enzo
E
Martegani
Ieso M
IM
Castro
Rogelio L
RL
Brandão
D020013:Calcium Signaling / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology*; D004789:Enzyme Activation; D044385:GTP-Binding Protein alpha Subunits / Q000378:metabolism; D005947:Glucose / Q000502:physiology; D051246:Glucose Transport Proteins, Facilitative; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D010766:Phosphorylation; D053499:Plasma Membrane Calcium-Transporting ATPases; D006180:Proton-Translocating ATPases / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
D044385:GTP-Binding Protein alpha Subunits; D051246:Glucose Transport Proteins, Facilitative; C090594:HXT1 protein, S cerevisiae; D009004:Monosaccharide Transport Proteins; C085484:PMC1 protein, S cerevisiae; C104463:SNF3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D006180:Proton-Translocating ATPases; D053499:Plasma Membrane Calcium-Transporting ATPases; C083907:Gpa2 protein, S cerevisiae; D000252:Calcium-Transporting ATPases; D005947:Glucose