[Microarray DNA chip in analyzing the association between HLA-DRB and advanced hepatosplenic schistosomiasis].
OBJECTIVE
To explore possible associations between host polymorphism of HLA class II genotypes and advanced hepatosplenic schistosomiasis japonica.


METHODS
45 advanced schistosomiasis patients (experimental group) and 44 age- and sex-matched patients with chronic schistosomiasis (control group) from the same area were investigated for their HLA class II gene DRB genotypes by genotyping the alleles using microarray DNA chip. The correlation of allele frequencies to advanced hepatosplenic schistosomiasis was compared for the two groups.


RESULTS
HLA-DRB1*04x exhibited markedly higher frequency in advanced patients than that in control group (P < 0.01, RR = 3.928). In contrast, the frequency of HLA-DRB1*15x in advanced patients was much lower when compared with that in control group (P < 0.01, RR = 0.050). Besides, the significant allele HLA-DRB1*15x displayed concurrence with allele DRB5*010x/020x. The linked gene haplotype DRB1*15x-DRB5*010x/020x showed significantly higher incidence in control group than in experimental group (P < 0.01).


CONCLUSIONS
Allele HLA-DRB1*04x is positively, while HLA-DRB1*15x is negatively, correlated with advanced hepatosplenic schistosomiasis.
Cheng
Yu-li
YL
Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Xu
Ming-xing
MX
Song
Wen-jian
WJ
Yang
Yan
Y
Liu
Wen-qi
WQ
Li
Yong-long
YL
Qiu
Min-yan
MY
Wu
Hai
H
D000328:Adult; D000368:Aged; D000483:Alleles; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008099:Liver / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011110:Polymorphism, Genetic; D012554:Schistosomiasis japonica / Q000235:genetics*; D013154:Spleen / Q000469:parasitology
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains