Pathophysiological basis for monitoring of whole heart conductance by 2-lead system.
BACKGROUND
The defibrillation threshold (DFT) is elevated during myocardial ischemia, but the underlying mechanism remains to be elucidated. The hypothesis tested by the present study was that whole heart conductance (WHC) is a determinant of DFT.


RESULTS
WHC was monitored across the longest diameter of the isolated perfused rat heart, using a 2-electrode instrument under various conditions including ischemia-reperfusion (IR). In the control study, WHC was influenced by the conductivity and flow rate of the solution. In IR, WHC decreased immediately after the onset of perfusion arrest in a single exponential manner, then declined again gradually. The second decrease was augmented and accelerated by pretreatment with 1.0 mmol/L heptanol (p<0.005) or high-[Ca2+]e (p<0.001), and was attenuated and delayed by pretreatment with 1.0 micromol/L verapamil (p<0.01). WHC after reperfusion was greater than the pre ischemic level. The postischemic increase in WHC was proportional to the ischemic interval and tissue water content as assessed by desiccation method.


CONCLUSIONS
Although time-dependent alterations in DFT in ischemic hearts may be attributable at least in part to dynamic changes in WHC, WHC should be interpreted carefully because it reflects many physiological factors such as coronary perfusion, electrical coupling of cardiac myocytes and tissue edema.
Yasuda
Yuichiro
Y
Department of Biosystemic Science and Medicine, Kyushu University, Graduate School of Medical Sciences, Kasuga, Fukuoka, Japan.
Maruyama
Toru
T
Nakamura
Hirofumi
H
Arita
Takeshi
T
Ueda
Norihiro
N
Harada
Mine
M
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004554:Electric Countershock / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D006325:Heart Atria / Q000503:physiopathology; D006329:Heart Conduction System / Q000187:drug effects / Q000503:physiopathology*; D019850:Heptanol / Q000494:pharmacology; D008297:Male; D017202:Myocardial Ischemia / Q000503:physiopathology*; D032383:Myocytes, Cardiac / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow; D015427:Reperfusion Injury / Q000503:physiopathology*; D013997:Time Factors; D014700:Verapamil / Q000494:pharmacology
D019850:Heptanol; D014700:Verapamil; D002118:Calcium