[Construction of molecular clone of chimeric porcine circovirus(PCV1-2) and identification of its infectivity].
Porcine circovirus type 2(PCV2) recently emerged as an important infectious pathogen for pigs in the world. Unfortunately, there is no efficient method to deal with PCV2 infection until now. In this study, chimeric porcine circovirus molecular clone (pSK2PCV1-2) was constructed by cloning capsid gene of PCV2 into the backbone of PCV1. PK-15 cells was transfected with pSK2PCV1-2 and then cultivated in plate for five passages. mRNA of PCV1 ORF1 and PCV2 ORF2 were detected in the fifth passage, but mRNA of PCV1 ORF2 and PCV2 ORF1 were not detected in the cells. On the other hand, capsid protein of PCV2 was also detected in the nucleolus of transfected cells by IIF. This study indicated that pSK2PCV1-2 could form infectious virus in transfected cells. It will provide base for further study on biological characteristic of chimeric porcine circovirus.
Cao
Sheng-bo
SB
State Key Laboratory of Agricultural Microbiology, Hua Zhong Agricultural University, Wuhan 430070, China. sbcao@mail.hzau.edu.cn
Sun
Min-xuan
MX
Liu
Xue-qin
XQ
Liu
Xin-wen
XW
Chen
Huan-chun
HC
D000818:Animals; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000821:virology*
D012333:RNA, Messenger