Millisia brevis gen. nov., sp. nov., an actinomycete isolated from activated sludge foam.
The taxonomic position of two mycolic-acid-producing actinomycetes, isolates J81T and J82, which were recovered from activated sludge foam, was clarified. Comparative 16S rRNA gene sequence studies indicated that the organisms formed a distinct lineage within the Corynebacterineae 16S rRNA gene tree. The taxonomic integrity of this group was underpinned by a wealth of phenotypic data, notably characteristic rudimentary right-angled branching. In addition, isolate J81T contained the following: meso-diaminopimelic acid, arabinose and galactose; N-glycolated muramic acid residues; a dihydrogenated menaquinone with eight isoprene units as the predominant isoprenologue; a fatty acid profile rich in oleic and palmitoleic acids and with relatively small proportions of myristic, stearic and tuberculostearic acids; mycolic acids with 44-52 carbons; and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides as major polar lipids. Strain J81T was found to have a chemotaxonomic profile that serves to distinguish it from representatives of all of the other taxa classified as belonging to the suborder Corynebacterineae. In the light of these data, it is proposed that the two isolates be classified in a novel monospecific genus. The name proposed for this taxon is Millisia brevis gen. nov., sp. nov.; strain J81T (=DSM 44463T = NRRL B-24424T) is the type strain of Millisia brevis.
Biotechnology Research Centre, La Trobe University, Bendigo, Victoria 3552, Australia.
Jacques A
JA
Soddell
Division of Biology, King George VIth Building, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Fiona M
FM
Stainsby
Biotechnology Research Centre, La Trobe University, Bendigo, Victoria 3552, Australia.
Kathryn L
KL
Eales
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, 38124 Braunschweig, Germany.
Reiner M
RM
Kroppenstedt
Biotechnology Research Centre, La Trobe University, Bendigo, Victoria 3552, Australia.
Robert J
RJ
Seviour
Division of Biology, King George VIth Building, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Michael
M
Goodfellow
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid
D012336:RNA, Ribosomal, 16S; D012722:Sewage