Isolation of human antibody repertoires with preservation of the natural heavy and light chain pairing.
The humoral immune system in higher vertebrates is unique in its ability to generate highly diverse antibody responses against most pathogens as well as against certain malignancies. Several technologies have been developed to exploit this vast source of potentially therapeutic antibodies, including hybridoma technology, phage display and yeast display. Here, we present a novel, high-throughput technology (the Symplex Technology) for rapid direct cloning and identification of human antigen-specific high-affinity antibodies from single antibody-producing cells of immune individuals. The utility of the technology was demonstrated by isolation of diverse sets of unique high-affinity antibodies against tetanus toxoid and influenza virus from immunized volunteers. Hence, the Symplex Technology is a new method for the rapid isolation of high-affinity antibodies directly from humans.
Meijer
Per-Johan
PJ
Symphogen A/S, Elektrovej, Building 375, DK-2800 Lyngby, Denmark.
Andersen
Peter S
PS
Haahr Hansen
Margit
M
Steinaa
Lucilla
L
Jensen
Allan
A
Lantto
Johan
J
Oleksiewicz
Martin B
MB
Tengbjerg
Kaja
K
Poulsen
Tine R
TR
Coljee
Vincent W
VW
Bregenholt
Søren
S
Haurum
John S
JS
Nielsen
Lars S
LS
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification* / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D019151:Peptide Library; D010802:Phylogeny; D013744:Tetanus Toxin / Q000276:immunology
D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D019151:Peptide Library; D013744:Tetanus Toxin