Separation of 1,4-benzodiazepines by micellar elektrokinetic capillary chromatography.
In this work the applicability of micellar elektrokinetic capillary chromatography (MECC) for the determination of benzodiazepines (BZD) has been studied. The applied method was used for the simultaneous separation of 8 BZDs (alprazolam, bromazepam, chlordiazepoxide, diazepam, flunitrazepam, medazepam, oxazepam, nitrazepam), and also for the study of stability in acidic medium. A fast and reliable method has been developed; using a separation buffer composed of sodium tetraborate 25 mM (pH 9.5), SDS (50 mM) and methanol (at least 12%) as an organic modifier.
Hancu
Gabriel
G
University of Medicine and Pharmacy, Târgu Mureş, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 540139 Târgu Mureş, Romania. g_hancu@rdslink.ro
Gáspár
Attila
A
Gyéresi
Arpád
A
D001569:Benzodiazepines / Q000737:chemistry / Q000302:isolation & purification*; D001881:Borates / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D001881:Borates; D001569:Benzodiazepines; D012967:Sodium Dodecyl Sulfate; C021755:tetraborate; D000432:Methanol