[Isolation of BADH gene promoter from Suaeda liaotungensis and its sequence analysis].
In this study, the 5' -flanking proximal region of stress-induced gene encoding betaine aldehyde dehydrogenase was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis. 1993 bp sequence was obtained by sequencing. The transcription start site, which localized at 62 bases upstream of the start ATG, was predicted using TSSP-TCM program. The functional elements were analysed by PLACE programm. The SlBADH gene promoter contains the basic elements: TATA-box, CAAT-box, and stress-induced elements: salt responsed element, cold, dehydration, ABA and frozen responsed elements, WUN responsed elements and HSE. Obtaining the promoter of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis provides a foundation for analyzing the stress-induced promoter elements, studying the relationship between structure and founction of the promoter, and investigating the molecular mechanism of BADH gene regulation.
College of Life Science, Liaoning Normal University, Dalian 116029, China. liqiuli@dl.cn
Qiu-Li
QL
Li
Yi
Y
Zhang
Hui
H
Yin
Dan
D
Li
D001483:Base Sequence; D050728:Betaine-Aldehyde Dehydrogenase / Q000235:genetics*; D019663:Chenopodiaceae / Q000201:enzymology* / Q000235:genetics; D003080:Cold Temperature; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D012492:Salts; D017422:Sequence Analysis, DNA; D024363:Transcription Initiation Site
D010940:Plant Proteins; D012492:Salts; D050728:Betaine-Aldehyde Dehydrogenase