[Effect of Gleditsia sinensis stings on growth inhibition and expression of PCNA and p53 in mice bearing uterine cervical carcinoma (U14)].
OBJECTIVE
To study the antitumor effect of the stings of Gleditsia sinensis on mice bearing uterine cervical carcinoma (U14) and the expression of PCNA (proliferating cell nuclear antigen) and p53.


METHODS
The effect of the ethanolic extract of G. sinensis stings on the inhibition rate of solid tumor and the life span of ascites tumor were calculated by the animal tumor model experiment in vivo. The positive cell numbers of PCNA and mutant p53 protein were measured by immunohistochemical SP method.


RESULTS
As compared with the control group, the ethanolic extract of G. sinensis stings (250, 500 and 1 000 mg x kg(-1) body weight, p.o.) and CTX (25 mg kg(-1) body weight, i.p.) administration significantly reduced the tumor weight of solid tumor and increased the life span of ascites tumor harboring mice (P < 0.01). The inhibition rate of solid tumor and the rate in life span were up to 47.44%, 59.49%, 63.92%, 73.42% and 52.21%, 67.26%, 78.76%, 95.58% respectively. Meanwhile,the expression of PCNA and mutant p53 protein also suppressed by ethanolic extract (P < 0.05, P < 0.01).


CONCLUSIONS
The stings of G. sinensis showed antitumor activity and its possible mechanism might be related with the expression inhibition of PCNA and mutant p53 protein.
College of Animal Science, Northwest Agriculture and Forestry University, Xi'an 712100, China.
Ling
L
Long
Guo-Xia
GX
Geng
Qing-Wang
QW
Li
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D031501:Gleditsia / Q000737:chemistry; D051379:Mice; D009154:Mutation; D009368:Neoplasm Transplantation; D010946:Plants, Medicinal / Q000737:chemistry; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D011897:Random Allocation; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology*
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53