Protective effect of triphlorethol-A from Ecklonia cava against ionizing radiation in vitro.
We studied the cytoprotective effect of triphlorethol-A against gamma-ray radiation-induced oxidative stress. In this study, hydrogen peroxide, which is a reactive oxygen species (ROS), was detected using 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA) assay. Triphlorethol-A reduced intracellular hydrogen peroxide generated by gamma-ray radiation. This compound provided protection against radiation-induced membrane lipid peroxidation and cellular DNA damage which are the main targets of radiation-induced damage. Triphlorethol-A protected the cell viability damaged by the radiation through inhibition of apoptosis. Triphlorethol-A reduced the expression of bax and activated caspase 3 induced by radiation, but recovered the expression of bcl-2 decreased by radiation. Taken together, the results suggest that triphlorethol-A protects cells against oxidative damage induced by radiation through reducing ROS.
Kang
Kyoung Ah
KA
Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju-si, Korea.
Zhang
Rui
R
Lee
Kyoung Hwa
KH
Chae
Sungwook
S
Kim
Bum Joon
BJ
Kwak
Young Sook
YS
Park
Jae Woo
JW
Lee
Nam Ho
NH
Hyun
Jin Won
JW
D000818:Animals; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects*; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D005720:Gamma Rays; D015227:Lipid Peroxidation / Q000187:drug effects; D000459:Phaeophyceae / Q000378:metabolism*; D010696:Phloroglucinol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D017382:Reactive Oxygen Species / Q000378:metabolism*
D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; C504400:triphlorethol-A; D010696:Phloroglucinol