Analysis of aspartate aminotransferase and alkaline phosphatase in crevicular fluid from implants with and without peri-implantitis.
OBJECTIVE
The aim of this investigation was to determine the presence of aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in crevicular fluid collected from implants with and without clinical and radiographic signs of peri-implantitis.


METHODS
There were 17 implants with symptoms of peri-implantitis in 12 subjects, including 4 females and 8 males, compared to 17 implants in 13 subjects, including 5 females and 8 males, with healthy peri-implant tissues. Filter paper strips were used to collect peri-implant crevicular fluid for 30 seconds in the base of the crevice/pocket. SPSS statistical software (SPSS, Inc., Chicago, IL) was used to determine AST and ALP activity.


RESULTS
The results showed that there was a significant difference in the activity of AST and ALP between the 2 study groups (P < 0.0001). AST activity was significantly associated with the amount of bleeding on probing (P = 0.02), but no statistical correlation was found between ALP activity and increased amount of bleeding on probing (P = 0.05).


CONCLUSIONS
Within the limits of this study, our results may suggest that peri-implant crevicular fluid analysis could be further investigated in longitudinal studies as a suitable diagnostic strategy in the evaluation of dental implants.
Paknejad
Mojgan
M
Department of Periodontics, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Emtiaz
Shahram
S
Khoobyari
Mohammad Mohsen
MM
Gharb
Mehrnoush Teimouri
MT
Yazdi
Mojtaba Tabatabaei
MT
D000469:Alkaline Phosphatase / Q000032:analysis*; D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000201:enzymology; D001219:Aspartate Aminotransferases / Q000032:analysis*; D015921:Dental Implants; D003773:Dental Plaque / Q000201:enzymology; D005260:Female; D005881:Gingiva / Q000201:enzymology; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D005884:Gingival Hemorrhage / Q000201:enzymology; D006801:Humans; D008297:Male; D016348:Osseointegration / Q000502:physiology; D010514:Periodontal Pocket / Q000201:enzymology; D010518:Periodontitis / Q000000981:diagnostic imaging / Q000201:enzymology*; D011859:Radiography
D015921:Dental Implants; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase