A new quasi-interpenetrating network formed by poly(N-acryloyl-tris-(hydroxymethyl)aminomethane and polyvinylpyrrolidone: separation matrix for double-stranded DNA and single-stranded DNA fragments by capillary electrophoresis with UV detection.
The preparation of a new separation matrix, quasi-interpenetrating networks (quasi-IPNs) formed by poly(N-acryloyl-Tris) (poly(tris-A)) and PVP, and its application for dsDNA and ssDNA fragments separation by CE with UV detection, are presented. This new quasi-IPN exhibited high sieving performance, good dynamic coating ability, and low viscosity. Single-base resolutions of dsDNA fragments (Rs = 0.92 for 123/124 bp) and ssDNA fragments (Rs = 0.65 for 123/124 base, Rs = 0.48 for 309/310 base) were achieved by using the quasi-IPN of poly(tris-A)/PVP (2% + 2%) solution in a 31 cm effective length linear polyacrylamide (LPA)-coated column. Single-base separation of dsDNA fragments (Rs = 0.92 for 123/124 bp) was also obtained within 28 min in a 46.7 cm effective length bare column at higher 160 V/cm electric field strength by using the same quasi-IPN solution. The RSD of the migration time measured for each DNA fragments was less than 1.5% in the bare column for nine continuous runs. The effects of temperature and electric field strength on the DNA separation were also investigated.
Wang
Qian
Q
Laboratory of Analytical Chemistry, State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China.
Xu
Xu
X
Dai
Lixin
L
D000179:Acrylates / Q000737:chemistry*; D000180:Acrylic Resins / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000302:isolation & purification; D004560:Electricity; D019075:Electrophoresis, Capillary / Q000379:methods*; D011205:Povidone / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D000099166:Polyacrylamides
D000179:Acrylates; D000180:Acrylic Resins; D004247:DNA; D004277:DNA, Single-Stranded; D011205:Povidone; D000099166:Polyacrylamides; C512194:poly(N-acryloyltris(hydroxymethyl)aminomethane)