Effect of gonadotropin-releasing hormone agonist and antagonist on proliferation and apoptosis of human luteinized granulosa cells.
The GnRH agonist leuprolide acetate (LA) inhibited DNA synthesis in epidermal growth factor-stimulated human granulosa luteinized cell cultures. This effect was blocked by the prior addition of a GnRH antagonist antide (ANT), and this compound per se was able to produce a stimulatory effect of DNA synthesis on basal conditions. Leuprolide acetate produced an increase in the percentage of apoptotic cells, and when these two factors were co-incubated, ANT blocked the apoptotic effect produced by LA.
Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científas y Técnicas, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina.
Alejandra M
AM
Vitale
Dalhia
D
Abramovich
Marina C
MC
Peluffo
Gabriela
G
Meresman
Marta
M
Tesone
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists* / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006107:Granulosa Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D016729:Leuprolide / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology
D009842:Oligopeptides; D007987:Gonadotropin-Releasing Hormone; C057022:iturelix; D016729:Leuprolide