Evaluation of human IgG subclass antibodies in the serodiagnosis of Paragonimiasis heterotremus.
Immunoglobulin G subclass antibodies (IgG1, IgG2, IgG3, and IgG4) responses to the excretory-secretory antigens of the lung fluke, Paragonimus heterotremus, were analyzed using the immunoblotting technique in an attempt to further improve the sensitivity and specificity for serodiagnosis of human paragonimiasis. Serum samples from patients with proven paragonimiasis, from patients with other parasitic infections, pulmonary tuberculosis and from healthy counterparts were analyzed. The results indicate that immunoblotting for the detection of IgG4 antibodies to an excretory-secretory product of P. heterotremus of an approximate molecular mass of 31.5 kDa, is the most reliable test. It gives accuracy, sensitivity, specificity, and positive and negative predictive values of 97.6%, 100%, 96.9%, 90% and 100%, respectively.
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand.
Chaisiri
C
Wongkham
Pewpan M
PM
Intapan
Wanchai
W
Maleewong
Masanao
M
Miwa
D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D010237:Paragonimiasis / Q000175:diagnosis*; D010238:Paragonimus / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000947:Antigens, Helminth; D007074:Immunoglobulin G