Microdevice DNA forensics by the simple tandem repeat method.
We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Full 16-locus multiplexes are verified on the multilane system. Multi-donor mixed samples are studied in the context of the limits of the laser-induced fluorescence detector and data-reduction software. The microdevice appears to be posed to outperform current capillary arrays in terms of stability and, through specialized sample loading, in the interpretation of complex mixtures.
Whitehead Institute for Biomedical Research, Nine Cambridge Center, MA 02142-1479, USA.
Nils
N
Goedecke
Brian
B
McKenna
Sameh
S
El-Difrawy
Elizabeth
E
Gismondi
Abigail
A
Swenson
Loucinda
L
Carey
Paul
P
Matsudaira
Daniel J
DJ
Ehrlich
D004247:DNA / Q000235:genetics*; D005554:Forensic Medicine; D006801:Humans; D020080:Tandem Repeat Sequences
D004247:DNA