Effects of glucocorticoids on polyamine metabolism in liver and spleen of guinea pig during sensitization.
Glucocorticoids are potent anti-inflammatory and immunosuppressive agents. As endogenous inhibitors of cytokine synthesis, glucocorticoids suppress immune activation and uncontrolled overproduction of cytokines, preventing tissue injury. Also, polyamine spermine is endogenous inhibitor of cytokine production (inhibiting IL-1, IL-6 and TNF synthesis). The idea of our work was to examine dexamethasone effects on the metabolism of polyamines, spermine, spermidine and putrescine and polyamine oxidase activity in liver and spleen during sensitization of guinea pigs. Sensitization was done by application of bovine serum albumin with addition of complete Freund's adjuvant. Our results indicate that polyamine amounts and polyamine oxidase activity increase during immunogenesis in liver and spleen. Dexamethasone application to sensitized and unsensitized guinea pigs causes depletion of polyamines in liver and spleen. Dexamethasone decreases polyamine oxidase activity in liver and spleen of sensitized guinea pigs, increasing at the same time PAO activity in tissues of unsensitized animals.
Institute of Biochemistry, Faculty of Medicine, University of Nis, Nis, Serbia and Montenegro. bjelakovic@junis.ni.ac.yu
G
G
Bjelakovic
D
D
Pavlovic
I
I
Stojanovic
T
T
Jevtovic
J
J
Nikolic
G
G
Kocic
D000818:Animals; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology*; D005620:Freund's Adjuvant; D005938:Glucocorticoids / Q000378:metabolism / Q000494:pharmacology*; D006168:Guinea Pigs; D007114:Immunization; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D008297:Male; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000276:immunology / Q000378:metabolism; D011073:Polyamines / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000276:immunology; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D000097667:Polyamine Oxidase
D016207:Cytokines; D003907:Dexamethasone; D005620:Freund's Adjuvant; D005938:Glucocorticoids; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D011700:Putrescine; D012710:Serum Albumin, Bovine; D013095:Spermidine; D013096:Spermine; D000097667:Polyamine Oxidase