Effect of gonadotrophin stimulation on mouse oocyte quality and subsequent embryonic development in vitro.
In-vivo-matured oocytes were collected from naturally ovulated and superovulated [pregnant mare's serum gonadotrophin (PMSG) + human chorionic gonadotrophin (HCG)] mice. Immature oocytes were retrieved from naturally cycling mice and from mice primed with PMSG. The percentages of cleavage and blastocyst formation were significantly different (P < 0.05) between in-vivo- and in-vitro-matured oocytes. Blastocyst formation rate was significantly higher (P < 0.05) in immature oocytes derived from PMSG-primed mice, and the percentages of oocytes with comet tails, and their length, were significantly higher and longer respectively in in-vitro-matured oocytes. Total cell numbers of blastocysts were also significantly different (P < 0.05) between in-vivo- and in-vitro-matured oocytes, but there were also no differences in ratio of trophectoderm (TE)/inner cell mass (ICM). In conclusion, in-vivo-matured mouse oocytes were more competent than those matured in-vitro, perhaps due to a lesser degree of DNA damage. Embryonic development capacity of in-vivo-matured oocytes is not promoted by ovarian stimulation. Gonadotrophin priming prior to immature mouse oocyte retrieval is beneficial to subsequent embryonic development.
Division of Reproductive Biology and Experimental Medicine, Department of Obstetrics and Gynecology, McGill University, Women's Pavilion F3, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Canada H3A 1A1.
Yue
Y
Wang
Sun-A
SA
Ock
Ri-Cheng
RC
Chian
D000818:Animals; D001562:Benzimidazoles; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D020552:Comet Assay; D053938:DNA Fragmentation / Q000187:drug effects / Q000502:physiology; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000592:standards; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011419:Propidium; D013480:Superovulation / Q000187:drug effects / Q000502:physiology
D001562:Benzimidazoles; D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D011419:Propidium; C017807:bisbenzimide ethoxide trihydrochloride