[Variation in evolutionary unstable regions of the chloroplast genome in plants obtained in anther culture of dihaploid wheat lines].
In dihaploid wheats, two evolutionarily unstable regions of the chloroplast genome were examined. These regions include the following genes, changes in which could be associated with albinism in anther culture: rbcL, encoding the large Rubisco subunit; psaA, encoding p700 apoprotein Ia; petA, encoding cytochrome f; atpB and atpE, encoding respectively beta and epsilon subunits of the CF1 ATPase complex; trnE, encoding glutamine tRNA; and cemA, encoding a cell membrane protein. Using PCR, we have shown that atpB was the gene most often not detected in the lines examined. These results suggest that regeneration of albino plants is accompanied by a deletion of a chloroplast DNA region harboring this gene.
G V
GV
Mozgova
P A
PA
Orlov
N V
NV
Shalygo
D018742:DNA, Chloroplast / Q000235:genetics*; D019143:Evolution, Molecular; D014644:Genetic Variation; D018745:Genome, Plant / Q000235:genetics*; D010858:Pigmentation / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011003:Ploidies; D014908:Triticum / Q000235:genetics*
D018742:DNA, Chloroplast; D010940:Plant Proteins