Expression of human DEC-205 (CD205) multilectin receptor on leukocytes.
DEC-205 (CD205) belongs to the macrophage mannose receptor family of C-type lectin endocytic receptors and behaves as an antigen uptake/processing receptor for dendritic cells (DC). To investigate DEC-205 tissue distribution in human leukocytes, we generated a series of anti-human DEC-205 monoclonal antibodies (MMRI-5, 6 and 7), which recognized epitopes within the C-type lectin-like domains 1 and 2, and the MMRI-7 immunoprecipitated a single approximately 200 kDa band, identified as DEC-205 by mass spectrometry. MMRI-7 and another DEC-205 mAb (MG38), which recognized the epitope within the DEC-205 cysteine-rich and fibronectin type II domain, were used to examine DEC-205 expression by human leukocytes. Unlike mouse DEC-205, which is reported to have predominant expression on DC, human DEC-205 was detected by flow cytometry at relatively high levels on myeloid blood DC and monocytes, at moderate levels on B lymphocytes and at low levels on NK cells, plasmacytoid blood DC and T lymphocytes. MMRI-7 F(ab')2 also labeled monocytes, B lymphocytes and NK cells similarly excluding reactivity due to non-specific binding of the mAb to FcgammaR. Tonsil mononuclear cells showed a similar distribution of DEC-205 staining on the leukocytes. DEC-205-specific semiquantitative immunoprecipitation/western blot and quantitative reverse transcriptase-PCR analysis established that these leukocyte populations expressed DEC-205 protein and the cognate mRNA. Thus, human DEC-205 is expressed on more leukocyte populations than that were previously assumed based on mouse DEC-205 tissue localization studies. The broader DEC-205 tissue expression in man is relevant to clinical DC targeting strategies and DEC-205 functional studies.
Mater Medical Research Institute, Dendritic Cell Laboratory, Aubigny Place, Raymond Terrace, Queensland 4101, Australia. mkato@mmri.mater.org.au
Masato
M
Kato
Kylie J
KJ
McDonald
Seema
S
Khan
Ian L
IL
Ross
Slavica
S
Vuckovic
Ke
K
Chen
David
D
Munster
Kelli P A
KP
MacDonald
Derek N J
DN
Hart
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D017951:Antigen Presentation / Q000276:immunology; D015703:Antigens, CD / Q000096:biosynthesis* / Q000276:immunology; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D004705:Endocytosis / Q000276:immunology; D005786:Gene Expression Regulation; D006801:Humans; D037181:Lectins, C-Type / Q000096:biosynthesis* / Q000276:immunology; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D015778:Minor Histocompatibility Antigens; D009928:Organ Specificity; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface