[GC-MS analysis of the fatty components of pollen Typhae before and after being carbonized].
OBJECTIVE
To study the changes of the fatty components of Pollen Typhae before and after being carbonized.


METHODS
Pollen Typhae and Pollen Typhae carbonisatus were extracted with petroleum ether (60-90 degrees C) respectively. The two kinds of extracts were analyzed by GC-MS after saponificated and methanolized, and their constituents were searched through NIST. The contents of the constituents were determined by method of normalization.


RESULTS
Either in Pollen Typhae or in Pollen Typhae carbonisatus, 32 components were identified, among which 20 components were the same and 6 were different respectively. Among the same components, the relative contents of 3-methyl-2-butenoic acid-2-phenylethyl ester, hexanedioic acid-dimethyl ester, dimethyl phthalate, diethyl phthalate, diphenylamine, sebacic acid dimethyl ester, 1,2-benzenedicarboxylic acid, ethyl methyl ester, methyl-2-ethylhexyl phthalate and diisooctyl phthalate etc. increased obviously, and the relative contents of nonanedioic acid-dimethyl ester, diisobutyl phthalate and stigmastan-3,5-dien etc. decreased greatly. Among the different components, 8-hydroxy-octanoic acid-methyl ester, 9-hydroxy-nonanoic acid-methyl ester, 10-octadecenoic acid-methyl ester, m-hydroxycinnamic acid-methyl ester,3-[4-( acetyloxy)-3-methoxyphenyl]2-propenoic acid-methyl ester and 11-octadecenoic acid-methyl ester were detected in Pollen Typhae, 3-hydroxyspirost-8-en-11-one, benzenepropanoic acid-methyl ester, 2,4-dimethylhexanedioic acid; 2,4-bis (1,1-dimethylethyl)-phenol, undecanedioic acid-dimethyl ester and 9,10-dihydroxy-octadecanoic acid-methyl ester were detected in Pollen Typhae carbonistatus.


CONCLUSIONS
The species and contents of the fatty components in Pollen Typhae changed before and after being carbonized, but their chemical types didn't change too much.
Beijing University of Chinese Medicine, Beijing 100102, China.
Hong-Fei
HF
Ma
Bin
B
Liu
Gui-Yan
GY
Zhang
Ren-Bing
RB
Shi
Chang-Hua
CH
Ma
Mi-Mi
MM
Yu
D002244:Carbon; D003993:Dibutyl Phthalate / Q000031:analogs & derivatives / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D010795:Phthalic Acids / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D032401:Typhaceae / Q000737:chemistry*
D005227:Fatty Acids; D010795:Phthalic Acids; C024629:dimethyl phthalate; D003993:Dibutyl Phthalate; D002244:Carbon; C025605:diisobutyl phthalate