A specific heat shock protein enhances the expression of mammalian olfactory receptor proteins.
Multiple trials failed to express significant amounts of olfactory receptors in heterologous cells as they are typically retained in the endoplasmic reticulum (ER). Evidence is accumulating that cell-type-specific accessory proteins regulate the folding of olfactory receptors, their exit from the ER, and the trafficking to the plasma membrane of the olfactory cilia where the receptors gain access to odorants. We found Hsc70t, a testis-enriched variant of the Hsp70 family of heat shock proteins which is specifically expressed in post-meiotic germ cells, in the olfactory epithelium of mouse and human. Cotransfected HEK293 cells with Hsc70t and different green fluorescent protein-tagged odorant receptors (ORs) from mouse and man showed a significantly enhanced OR expression. Hsc70t expression also changed the amount of cells functionally expressing olfactory receptors at the cell surface as the number of cells responding to odorants in Ca2+-imaging experiments significantly increased. Our results show that Hsc70t helps expression of ORs in heterologous cell systems and helped the characterization of an "orphan" human olfactory receptor.
Neuhaus
Eva M
EM
Department of Cell Physiology, Ruhr-Universitaet Bochum, Universitaetsstrasse 150, 44780 Bochum, Germany. eva.neuhaus@rub.de
Mashukova
Anastasia
A
Zhang
Weiyi
W
Barbour
Jon
J
Hatt
Hanns
H
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology; D050883:HSC70 Heat-Shock Proteins / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009831:Olfactory Mucosa / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D009928:Organ Specificity; D018035:Receptors, Odorant / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D013737:Testis / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D013997:Time Factors
D050883:HSC70 Heat-Shock Proteins; D018035:Receptors, Odorant