Application of lectin microarray to crude samples: differential glycan profiling of lec mutants.
We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lec mutants. Each of the cell surface extracts gave a significantly different profile from that of the parental CHO cells in a manner reflecting denoted biosynthetic features. Hence, the developed lectin microarray system is considered to be fully applicable for differential glycan profiling of crude samples.
Ebe
Youji
Y
Nano/Bio Science Research Lab., Yokohama Technical Center, Moritex Corporation, 1-3-3 Azamino-Minami, Aoba-ku, Yokohama, Kanagawa 225-0012.
Kuno
Atsushi
A
Uchiyama
Noboru
N
Koseki-Kuno
Shiori
S
Yamada
Masao
M
Sato
Takashi
T
Narimatsu
Hisashi
H
Hirabayashi
Jun
J
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006031:Glycosylation; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D040081:Protein Array Analysis; D011485:Protein Binding
D037102:Lectins; D011134:Polysaccharides