Ethanoligenens harbinense gen. nov., sp. nov., isolated from molasses wastewater.
Two strictly anaerobic bacterial strains (YUAN-3T and X-29) were isolated from anaerobic activated sludge of molasses wastewater in a continuous stirred-tank reactor. The strains were Gram-positive, non-spore-forming, mesophilic and motile. Cells were regular rods (0.4-0.8 x 1.5-8.0 microm) and occurred singly, in pairs and sometimes in chains of up to eight. Autoaggregative and autofluorescent growth of strain YUAN-3T and non-aggregative growth of strain X-29 were observed at 20-44 degrees C and pH 3.5-9.0. Both strains hydrolysed gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. Fermentation end products formed from glucose were acetate, ethanol, hydrogen and carbon dioxide. The predominant cellular fatty acids were the branched-chain fatty acids iso-C(16 : 0) (44.18 %) and iso-C(12 : 0) (26.67 %). The DNA G+C contents of strains YUAN-3T and X-29 were 47.8 and 49.0 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represent a novel phyletic sublineage within the Clostridium cellulosi rRNA cluster, with <92 % 16S rRNA gene sequence similarity to currently known species. On the basis of polyphasic evidence from this study, it is proposed that the unknown bacterium should be classified in a new genus as a novel species, Ethanoligenens harbinense gen. nov., sp. nov. The type strain of Ethanoligenens harbinense is YUAN-3T (=JCM 12961T = CGMCC 1.5033T).
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Defeng
D
Xing
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Nanqi
N
Ren
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Qiubo
Q
Li
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Ming
M
Lin
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Aijie
A
Wang
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Lihua
L
Zhao
D000693:Anaerobiosis; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D008965:Molasses / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D014871:Water Microbiology
D012336:RNA, Ribosomal, 16S