Addition of glycerol for improved methylation linkage analysis of polysaccharides.
A presolubilization procedure with the use of glycerol is shown to be applicable for the structural analysis of polysaccharides. Neutral, acidic, high-molecular-weight and low-molecular-weight polysaccharides were solubilized in glycerol prior to methylation and subsequent linkage analysis by GC-MS. All four types of polysaccharides showed significant increases in derivatization following presolubilization as measured by recovery of partially methylated alditol acetates.
Wyeth Vaccine Research and Development, 4300 Oak Park, Sanford, NC 27330, USA. kimj9@wyeth.com
John S
JS
Kim
Bradley L
BL
Reuhs
Francis
F
Michon
Raymond E
RE
Kaiser
Rasappa G
RG
Arumugham
D008401:Gas Chromatography-Mass Spectrometry; D005990:Glycerol / Q000737:chemistry*; D008745:Methylation; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry*; D012995:Solubility; D013402:Sugar Alcohols / Q000737:chemistry
D011134:Polysaccharides; D013402:Sugar Alcohols; D005990:Glycerol