Further validation of the HPCD-technique for the evaluation of PAH microbial availability in soil.
There is currently considerable scientific interest in finding a chemical technique capable of predicting bioavailability; non-exhaustive extraction techniques (NEETs) offer such potential. Hydroxypropyl-beta-cyclodextrin (HPCD), a NEET, is further validated through the investigation of concentration ranges, differing soil types, and the presence of co-contaminants. This is the first study to demonstrate the utility of the HPCD-extraction technique to predict the microbial availability to phenanthrene across a wide concentration range and independent of soil-contaminant contact time (123 d). The efficacy of the HPCD-extraction technique for the estimation of PAH microbial availability in soil is demonstrated in the presence of co-contaminants that have been aged for the duration of the experiment together in the soil. Desorption dynamics are compared in co-contaminant and single-PAH contaminated spiked soils to demonstrate the occurrence of competitive displacement. Overall, a single HPCD-extraction technique proved accurate and reproducible for the estimation of PAH bioavailability from soil.
Doick
Kieron J
KJ
Department of Environmental Science, Institute of Environmental and Natural Science, Lancaster University, Bailrigg, Lancaster, LA1 4YQ, UK.
Clasper
Paula J
PJ
Urmann
Karina
K
Semple
Kirk T
KT
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000327:Adsorption; D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D004784:Environmental Monitoring / Q000379:methods*; D010616:Phenanthrenes; D006109:Poaceae; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000378:metabolism; D011721:Pyrenes; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*; D013995:Time; D014197:Trees; D047392:beta-Cyclodextrins
D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D012989:Soil Pollutants; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; C031181:phenanthrene; C030984:pyrene