[Analysis of molecular profiles among Trypanozoon species and subspecies by MGE-PCR method].
OBJECTIVE
To analyze the relationship between genetic variability and evolution among Trypanosoma brucei (including T. b. brucei, T. b. rhodesiense and T. b. gambiense), T. evansi and T. equiperdum isolates.


METHODS
Genomic DNAs of 26 trypanosome isolates were amplified by a mobile genetic elements (MGE) -PCR technique and cluster analysis was performed based on the molecular profiles with Neighbor-Joining method.


RESULTS
The genetic variability among trypanosome isolates examined was obvious with an average genetic distance of 41.2% (ranged from 0 to 100%). Similarity coefficient among T. brucei isolates was 41.15% which was lower than that between T. evansi and T. equiperdum isolates. The closest relationship was found between T. evansi and T. brucei isolates with a similarity coefficient of 62.94%. The genetic variability between T. b. rhodesiense and T. b. brucei isolates was higher than that among T. b. gambiense isolates.


CONCLUSIONS
Species and subspecies in Trypanozoon displayed a higher genetic variability; T. equiperdum isolates collected from China and from South America, and T. evansi isolates from China and from South America, should have a similar origin.
Center for Parasitic Organisms, School of Life Sciences, Zhongshan (Sun Yat-Sen) University, Guangzhou, China.
Feng-jun
FJ
Li
Jia-yu
JY
Zheng
Wan-zhong
WZ
Jia
Zhao-rong
ZR
Lun
D000818:Animals; D016000:Cluster Analysis; D019143:Evolution, Molecular; D014644:Genetic Variation; D020071:Interspersed Repetitive Sequences / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014345:Trypanosoma / Q000145:classification* / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000145:classification / Q000235:genetics; D014347:Trypanosoma brucei gambiense / Q000145:classification / Q000235:genetics; D016833:Trypanosoma brucei rhodesiense / Q000145:classification / Q000235:genetics