HLA-B27 testing in Thai patients using the PCR-SSP technique.
We develop the HLA-B27 test kit using the PCR-SSP technique. Five hundred forty blood samples were tested for HLA-B27 by microlymphocytotoxicity test (LCT) and PCR-SSP. It was found that 127 (23.5%) and 134 (24.8%) of these samples were positive for HLA-B27 by LCT and PCR-SSP, respectively. The sensitivity and specificity of the PCR-SSP were 94.8 and 100%, respectively, when using LCT as the standard method. The PCR-SSP positive predictive value was 100%, negative predictive value was 98.3%, and a concordance rate of 98.7%. This study shows that the PCR-SSP is simple, convenient, and a more cost-effective in-house test kit.
Nathalang
O
O
Department of Pathology, Phramongkutklao College of Medicine, Bangkok, Thailand. oytipnti@hotmail.com
Tantimavanich
S
S
Nillakupt
K
K
Arnutti
P
P
Jaruchaimontree
C
C
D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D015796:HLA-B27 Antigen / Q000032:analysis*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic / Q000191:economics; D012680:Sensitivity and Specificity; D013785:Thailand / Q000453:epidemiology
D015796:HLA-B27 Antigen; D011933:Reagent Kits, Diagnostic